Consulta: subjectFacets:"Somatic embryogenesis"
Registros recuperados: 42
Data/hora: 09/06/2024 08:36:09

Producción de embriones somáticos de Bursera linanoe (La Llave) Rzed., Calderón &amp; Medina, en biorreactores de sistema de inmersión temporal.
Provedor de dados: 32
Autores: Vázquez Cisneros, Isaac.
El linaloe es un árbol conocido por su aroma agradable; la madera se utiliza para fabricar artesanías, y los frutos para extracción de aceite esencial, sin embargo, su aprovechamiento ha sido descontrolado y no existe manejo forestal para esta especie, lo cual reduce la población natural. Se plantea mejorar la metodología de embriogénesis somática tradicional mediante un sistema de inmersión temporal, así mismo, evidenciar el proceso embriogénico indirecto y secundario, finalmente comprobar la producción de metabolitos secundarios de interés. Se utilizó la línea embriogénica LEL 61-4, y un medio de cultivo base MS complementado con 30 g L-1 sacarosa, 0.022 μM bencil adenina y 0.027 μM ácido naftalenacético, con temperatura de 25 ± 2 ºC y fotoperiodo de 16...
Palavras-chave: Linaloe; Burseraceae; Sistema de inmersión temporal; Calidad de luz; Embriogénesis somática; Temporary immersion system; Light type; Somatic embryogenesis; Forestal; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2236

Expression of RNA polymerase IV and V in Oryza sativa
Provedor de dados: 69
Autores: Moo,Leydi de Rocio Canche; González,Angela Kú; Rodríguez-Zapata,Luis Carlos; Suarez,Victor; Castaño,Enrique.
RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral control. However, RNA pol IV and V are yet to be study in model monocot systems like Oryza sativa that can provide further information on genetic silence mechanism in plats. We show the expression pattern of these polymerases in tissues extracts of Oryza sativa. Detectable amounts of these polymerases are found in specific adult plant tissues and particularly expressed during somatic embryogenesis but not during early stages of normal embryo development. The use...
Tipo: Journal article
Palavras-chave: Oryza sativa; RNA pol IV; RNA pol V; Somatic embryogenesis; Zygotic embryogenesis.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200009

Screening for propagation suitability in vitro of different Cyclamen species
Provedor de dados: 69
Autores: Seyring,Martina; Ewald,Aloma; Mueller,Anke; Haensch,Klaus-Thomas.
In the present study we examined the possibility of propagating different Cyclamen species (C. africanum Boiss. and Reut., C. cilicium Boiss. and Heldr., C. coum Mill., C. hederifolium Ait., C. persicum Mill., C. purpurascens Mill.) including some of their subspecies and cultivars in vitro using explants of adult plants. For this purpose two protocols have been applied to eleven genotypes combined with mostly four explant types (placentas with ovules, leaves, petioles and peduncles). The use of these protocols has given rise to either somatic embryo-like structures and/or adventitious shoots in all genotypes. This way it was possible to propagate each of the examined genotypes in vitro using explants of adult plants in a time less than one year. These...
Tipo: Journal article
Palavras-chave: Propagation; Shoot regeneration; Somatic embryogenesis; Species.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400010

Influence of 2,4-D and BAP on callus growth and the subsequent regeneration of somatic embryos in long-term cultures of Pelargonium x domesticum cv. Madame Layal
Provedor de dados: 69
Autores: Haensch,Klaus-Thomas.
This long-term study demonstrates for the first time that it is possible to propagate embryogenic cultures in pelargoniums and to subsequently initiate the differentiation of embryos using the cultivar Madame Layal (Pelargonium x domesticum). Propagation of callus was only possible with combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP), which gave rise to embryos from the primary culture stage on. However, the propagation of cells, as well as the differentiation of embryos, was inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation...
Tipo: Journal article
Palavras-chave: Auxin; Cytokinin; Propagation; Somatic embryogenesis.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100007

Plant regeneration via indirect somatic embryogenesis and optimisation of genetic transformation in Coffea arabica L. cvs. Caturra and Catuaí
Provedor de dados: 69
Autores: Gatica-Arias,Andrés M; Arrieta-Espinoza,Griselda; Espinoza Esquivel,Ana M.
A protocol for Coffea arabica L. cvs. Caturra and Catuaí plant regeneration via indirect somatic embryogenesis (ISE) was established. Furthermore, a biolistic mediated genetic transformation protocol was optimized for Catuaí callus aggregates. Maximum callus induction was obtained when Caturra (87%) and Catuaí (67%) leaves were cultured on Murashige and Skoog medium with 18.56 µM kinetin and 4.52 µM2,4-dichlorophenoxyacetic acid (2,4-D). Catuaí suspension cultures were established from embryogenic callus using liquid proliferation CP and Sli media and diffused light and darkness. The higher suspension cultures fresh weight was obtained using Erlenmeyer (1425.4 ± 354.9 mg) than Recipient for Automated Temporary Immersion System (RITA®) (518.6 ± 55.1 mg),...
Tipo: Journal article
Palavras-chave: Bioreactor; Coffee; Genetic transformation; Somatic embryogenesis; Suspension cultures.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100010

Induction of somatic embryogenesis as an example of stress-related plant reactions
Provedor de dados: 69
Autores: Zavattieri,Maria Amely; Frederico,António Miguel; Lima,Mónica; Sabino,Rui; Arnholdt-Schmitt,Birgit.
In this review, we address the role of stress as one of the principal causes for a cell or tissue to change its pre-existing somatic program, reprogramming itself to express the embryogenic pathway. The focus of this paper is the effect of different stress conditions on the induction phase of plant somatic embryogenesis, as well as the development of embryogenic competence as a result of the applied stresses. We also present a variety of data that link plant somatic embryogenesis, DNA methylation and oxidative stress response.
Tipo: Journal article
Palavras-chave: Cell reprogramming; Reactive oxygen species; Somatic embryogenesis; Stress.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100012

Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines
Provedor de dados: 69
Autores: González,Germán Andrés; Pacheco,María Gabriela; Oneto,Cecilia Décima; Etchart,Valeria J; Kandus,Mariana V; Salerno,Juan Carlos; Eyherabide,Guillermo; Presello,Daniel; Lewi,Dalia Marcela.
Somatic embryogenesis, which is still the method of choice for tissue culture, regeneration and transformation of maize, is largely considered highly genotype-dependent. The Hi II, a highly embryogenic genotype, has been extensively used in transformation protocols. However, this is not an inbred line; instead, it has a proportion of the undesirable A-188 background, and the progeny segregates for phenotypic characteristics and shows poor agronomic performance. In an effort to identify genotypes that combine a high somatic embryogenic response and good agronomic performance, we evaluated 48 advanced inbred lines developed at INTA. Callus development and somatic embryogenesis capacity were measured in 200 immature embryos per line. Embryogenic capacity [EC...
Tipo: Journal article
Palavras-chave: Callus; Inbred lines; Maize; Molecular markers; Regeneration; Somatic embryogenesis.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100009

Selection of reference genes for normalization of quantitative real-time PCR in cell cultures of Cyclamen persicum
Provedor de dados: 69
Autores: Hoenemann,Claudia; Hohe,Annette.
As a prerequisite for gene expression analyses in cell cultures of the ornamental crop Cyclamen persicum basic parameters for quantitative real-time polymerase chain reaction (qRT-PCR) have been established including the selection of reference genes using the software tools &amp;#8216;geNorm&amp;#8217; and &amp;#8216;NormFinder&amp;#8217;. Five potential reference genes have been tested (elongation factor tu (Ef-Tu), putative ABC transporter ATPase, putative conserved oligomeric Golgi (COG) complex component, V-ATPase G subunit 1 and Histone H3-K9 methyltransferase 4 (H3-K9-HMTase 4)). &amp;#8216;NormFinder&amp;#8217; as well as &amp;#8216;geNorm&amp;#8217; identified Ef-Tu to be the least stable reference gene while the ranking of the most stable genes...
Tipo: Journal article
Palavras-chave: Gene expression analysis; In vitro propagation; Primer design; Somatic embryogenesis.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100012

AgNO3: a potential regulator of ethylene activity and plant growth modulator
Provedor de dados: 69
Autores: Kumar,Vinod; Parvatam,Giridhar; Ravishankar,Gokare Aswathanarayana.
The aim of this review is to critically analyze the role of silver nitrate (AgNO3) in modulating plant growth and development. In recent years, basic studies on ethylene regulation opened new vistas for applied research in the area of micro-propagation, somatic embryogenesis, in vitro flowering, growth promotion, fruit ripening, and sex expression. Silver nitrate has proved to be a very potent inhibitor of ethylene action and is widely used in plant tissue culture. Few properties of silver nitrate such as easy availability, solubility in water, specificity and stability make it very useful for various applications in exploiting plant growth regulation and morphogenesis in vivo and in vitro. Silver ion mediated responses seem to be involved in polyamines,...
Tipo: Journal article
Palavras-chave: Calcium; Ethylene; Morphogenesis; Polyamines; Silver nitrate; Somatic embryogenesis.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200008

Characterization of the third SERK gene in pineapple (Ananas comosus) and analysis of its expression and autophosphorylation activity in vitro
Provedor de dados: 74
Autores: Ma,Jun; He,Yehua; Hu,Zhongyi; Xu,Wentian; Xia,Jingxian; Guo,Cuihong; Lin,Shunquan; Chen,Chengjie; Wu,Chenghou; Zhang,Junli.
Two somatic embryogenesis receptor-like kinase genes (identified as AcSERK1 and AcSERK2) have previously been characterized from pineapple (Ananas comosus). In this work, we describe the characterization of a third gene (AcSERK3) in this family. AcSERK3 had all the characteristic domains and shared extensive sequence homology with other plant SERKs. AcSERK3 expression was studied by in situ hybridization and quantitative real-time PCR to analyze its function. Intense in situ hybridization signals were observed only in single competent cells and competent cell clusters; no hybridization signal was detected in the subsequent stages of somatic embryogenesis. AcSERK3 was highly expressed in embryogenic callus compared to other organs, e.g., 20-80 fold more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AcSERK3; Autophosphorylation activity; Expression analysis; Prokaryotic expression; Somatic embryogenesis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400009

Identification of histone methylation modifiers and their expression patterns during somatic embryogenesis in Hevea brasiliensis
Provedor de dados: 74
Autores: Li,Hui-Liang; Guo,Dong; Zhu,Jia-Hong; Wang,Ying; Peng,Shi-Qing.
Abstract Histone methylation plays a crucial role in various biological processes, from heterochromatin formation to transcriptional regulation. Currently, no information is available regarding histone methylation modifiers in the important rubber-producing plant Hevea brasiliensis. Here, we identified 47 histone methyltransferase (HMT) genes and 25 histone demethylase (HDM) genes as possible members of the histone methylation modifiers in the rubber tree genome. According to the structural features of HMT and HDM, the HbHMTs were classified into two groups (HbPRMs and HbSDGs), the HbHDMs have two groups (HbLSDs and HbJMJs). Expression patterns were analyzed in five different tissues and at different phases of somatic embryogenesis. HbSDG10, 21, 25, 33,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hevea brasiliensis; Histone methylation; Histone methyltransferase; Histone demethylase; Somatic embryogenesis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100301

Direct somatic embryogenesis and organogenesis pathway of plant regeneration can seldom occur simultaneously within the same explant of sorghum
Provedor de dados: 30
Autores: Girijashankar, V.
Palavras-chave: Somatic embryos; Transgenics; Somatic embryogenesis; Auxins; Regeneration; Protocols; Sorghum; Cytokinins; Embryonic development; Transgenic plants.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3300

Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra
Provedor de dados: 86
Autores: Morais-Lino,Lucymeire Souza; Santos-Serejo,Janay Almeida dos; Silva,Sebastião de Oliveira e; Santana,José Raniere Ferreira de; Kobayashi,Adilson Kenji.
The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Musa; Culture media; Propagation; Somatic embryogenesis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000010

Assessment of somaclonal variation in indirect morphogenesis-derived plants of Arracacia xanthorrhiza
Provedor de dados: 86
Autores: Vitamvas,Jan; Viehmannova,Iva; Cepkova,Petra Hlasna; Mrhalova,Hana; Eliasova,Katerina.
Abstract: The objective of this work was to induce and detect somaclonal variation in arracacha (Arracacia xanthorrhiza) plants regenerated via indirect morphogenesis, in order to evaluate the potential of this technique to produce new genotypes for breeding purposes of this crop. Calli were induced from petiole segments on Murashige &amp; Skoog (MS) medium supplied with 0.1 mg L-1 2,4-dichlorophenoxyacetic acid. The regeneration of plants via indirect morphogenesis was carried out on half-strength MS medium without plant growth regulators. Fifteen randomly chosen plants were subjected to flow cytometry and “inter-simple sequence repeat” (ISSR) analysis. Ploidy level remained stable in all tested regenerants (2n=4x=44), with no changes in the genome....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arracacha; ISSR; Plant breeding; Ploidy level; Somatic embryogenesis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102200

Physiological and biochemical changes during organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant
Provedor de dados: 85
Autores: Guan,Z-J; Lu,S-B; Huo,Y-L; Guo,B; Guan,Z-P; Wei,Y-H.
Leaf explants of the HBsAg-transgenic cherry tomato (Solanum lycopersicum) mutant were cultured on Murashige and Skoog (MS) basal medium, supplemented with 1.0 mg/L 6-BA and 0.05 mg/L IAA for callus induction, to clarify the physiological and biochemical characteristics of morphogenesis development. Therefore, the physiological and biochemical changes during the development of organogenic shoots and somatic embryos in the mutant were studied. Superoxide dismutase (SOD) activities of the mutant had only one peak value on the 21st day. Peroxidase (POD) activities of the mutant declined less sharply since the explants were cultured. IAA oxidase activity of the mutant increased steadily until 42 days from culturing and then decreased sharply. Malondialdehyde...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physiological; Biochemical; Organogenesis; Somatic embryogenesis; HBsAg-transgenic; Cherry tomato; Mutant.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000200013

Histocytological examination on organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant
Provedor de dados: 85
Autores: Guan,Z-J; Guo,B; Huo,Y-L; Dai,J-K; Wei,Y-H.
The initiation and development of organogenic buds and somatic embryos in HBsAg-transgenic cherry tomato (Lycopersicum esculentum var. cerasiforme) mutant were studied histologically. The leaf explants of the mutant were cultured on Murashige &amp;amp; Skoog (MS) basal medium supplemented with 6-BA 1.0 mg/L and IAA 0.05 mg/L for callus induction. Histological studies on the leaf explants of the mutant at various developmental stages revealed that organogenic buds first appeared in the axillary position of explants on the 14th cultured day, and then somatic embryos formed in the same mutant explants after 35 days of culture. Transmission electron microscopy and scanning electron microscopy indicated that there were significant changes in morphology and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histocytological; Organogenesis; Somatic embryogenesis; HBsAg; Transgenic; Cherry tomato; Mutant.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100008

In vitro somatic embryogenesis and adventitious root initiation have a common origin in eggplant (Solanum melongena L.)
Provedor de dados: 88
Autores: Tarré,Erika; Magioli,Claudia; Margis-Pinheiro,Márcia; Sachetto-Martins,Gilberto; Mansur,Elisabeth; Santiago-Fernandes,Lygia D. R.
Somatic embryogenesis was induced from cotyledon explants of eggplant cultured on MS medium supplemented with 54 µM NAA. Anatomical analysis of somatic embryo initiation and development was performed during the first four weeks. Proembryo formation was observed after the second day of culture, directly from perivascular cells or via pro-embryogenic masses derived from indeterminate meristematic masses (IMMs) originated in the vascular tissue. Those IMMs also gave rise to root primordia after 10 days of culture. The origin of embryos is discussed as well as the similarities between somatic embryogenesis and adventitious root formation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Development; Solanum melongena; Somatic embryogenesis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042004000100010

Germinação in vitro e embriogênese somática a partir de embriões imaturos de canela sassafrás (Ocotea odorifera Mez)
Provedor de dados: 88
Autores: SANTA-CATARINA,CLAUDETE; MACIEL,SCHEILA DA CONCEIÇÃO; PEDROTTI,ENIO LUIZ.
Embriões zigóticos em diferentes estádios de desenvolvimento foram utilizados para avaliar o potencial de germinação e competência embriogênica in vitro. Frutos coletados entre 15 e 360 dias após a antese foram avaliados em relação ao diâmetro (mm), comprimento (mm) e massa fresca (g). Para avaliar a percentagem de germinação in vitro, embriões zigóticos em diferentes estádios de desenvolvimento foram inoculados em meio de cultura composto de sais minerais de Murashigue &amp;amp; Skoog (MS), ou em meio MS suplementado com 4,4 miM de BAP, sacarose (3%), ágar (0,6%), carvão ativado (0,15%). Para a indução de embriogênese somática, eixos embrionários em diferentes estádios de desenvolvimento foram inoculados em meio de cultura MS, suplementado com 2,4-D (0,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zygotic embryos; Somatic embryogenesis; In vitro germination; Woody plant; Lauraceae.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000500004

Advances observed in papaya tree propagation
Provedor de dados: 94
Autores: Costa,Adelaide de Fátima Santana da; Abreu,Emanuel Felipe Medeiros; Schmildt,Edilson Romais; Costa,Aureliano Nogueira da; Schmildt,Omar.
Abstract  Papaya (Carica papaya L.) is a fruit tree of great economic and food interest worldwide. Its propagation can occur both sexually and asexually. Traditionally, it is propagated by seeds; however, vegetative propagation has been often used with the use of in vitro and ex vitro culture techniques. In vegetative propagation, the cutting method has been extensively explored. Research has also been developed using grafting. In recent years, papaya cultivation has also become a model for other fruit plants in the use of biotechnological techniques aiming applications ranging from germplasm conservation to genetic transformation of plants. Since the beginning of the 1970s, the culture of papaya tree tissues has been intensely studied, allowing greater...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carica papaya L.; Propagation of papaya; Vegetative propagation; Somatic embryogenesis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000501001

In vitro regeneration and morphogenesis of somatic embryos of cassava
Provedor de dados: 124
Autores: Vidal,Ádila Melo; Costa,Maria Angélica Pereira de Carvalho; Souza,Antônio da Silva; Almeida,Weliton Antonio Bastos de; Souza,Fernanda Vidigal Duarte.
In this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 µM copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 µM BAP. The highest frequency of calluses and the greatest number of embryos per explant were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cassava; Somatic embryogenesis; Regulators-plants.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000300017

Induction of somatic embryogenesis and plant regeneration of sugarcane cv. RB925345 from somatic embryos
Provedor de dados: 22
Autores: Almeida, Rodrigo de Oliveira; Silva, Sérgio Delmar dos Anjos e; Dutra, Leonardo Ferreira.
The aim of this study was to establish a protocol for induction of somatic embryogenesis and regeneration of somatic embryos of sugarcane (cultivar RB925345). Best results was obtained using the concentration of 13.5 µM L-1 2,4-dichlorophenoxyacetic acid, 45 days of incubation in induction medium, one subculture and 15 days of incubation in regeneration medium, providing 47.5% of embryogenic calli and 38.9% of plants regenerated. Adjust are necessary in this protocol to increase the rate of embryogenic calli production and regeneration of somatic embryos.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharum spp.; 2 4-dichlorophenoxyacetic acid; Plant tissue culture; Somatic embryogenesis.
Ano: 2018
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/14165

Clonal Arabica coffee resistant to Meloidogyne paranaensis and damage threshold on plants development
Provedor de dados: 63
Autores: Fatobene,Bárbhara Joana dos Reis; Gonçalves,Wallace; Oliveira,Claudio Marcelo Gonçalves; Guerreiro Filho,Oliveiro.
ABSTRACT: Plants of Coffea arabica Icatu IAC 925 germplasm show resistance to Meloidogyne paranaensis under field conditions, but with variable rates of segregation. One mother plant, IAC 3, of Icatu IAC 925 was evaluated under controlled conditions through the reaction of its clones, obtained by somatic embryogenesis. Reproduction of nematodes in clones were compared with an open-pollinated progeny from the same mother plant, C. arabica cultivar Mundo Novo IAC 515-20, susceptible to nematodes, and Clone IAC 1, also susceptible and obtained by somatic embryogenesis. Open-pollinated progeny showed segregation to resistance and was classified as susceptible according to the reproduction factor. The average number of nematodes (eggs and J2) per gram of roots...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Root-knot nematodes; Resistant variety; Somatic embryogenesis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001300227

Regeneration of soybean via embryogenic suspension culture
Provedor de dados: 63
Autores: Droste,Annette; Leite,Paulo César Pimentel; Pasquali,Giancarlo; Mundstock,Elsa Cristina de; Bodanese-Zanettini,Maria Helena.
In an attempt to establish an alternative plant regeneration system for soybean [Glycine max (L.) Merrill] cultivars used in Brazilian breeding programs, ten genotypes were tested for their embryogenic potential. Cotyledons were removed as explants from immature seeds harvested from field-grown plants. After 45 days on induction medium, the number of responding cotyledons and the number of somatic embryos per immature cotyledon were evaluated. The percentage of explants that produced somatic embryos varied from 1 to 70% among cultivars. The average number of somatic embryos produced per cotyledon pair ranged from 0.01 to 10.3 with a mean of 3.4. Suspension cultures were initiated with three Agrobacterium tumefaciens susceptible cultivars. Suspensions were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Somatic embryogenesis; Liquid medium; Plant tissue culture.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000400017

POLYPHENOLS DISTRIBUTION AND RESERVE SUBSTANCES ANALYSIS IN CACAO SOMATIC EMBRYOGENESIS
Provedor de dados: 37
Autores: GALLEGO RÚA,Adriana María; HENAO RAMÍREZ,Ana María; URREA TRUJILLO,Aura Inés; ATEHORTÚA GARCÉS,Lucía.
In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Histology; Reserve accumulation; Recalcitrance; Somatic embryogenesis.
Ano: 2016
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2016000200002

Evaluation of soybean cultivars on the embryogenic and organogenic potential - doi: 10.4025/actasciagron.v33i1.12344
Provedor de dados: 1
Autores: Texeira, Leandra Regina; UEM; Braccini, Alessandro De Lucca e; UEM; Churata, Bruno Grimaldo Martinho; UFPR; Vieira, Elisa Serra Negra; Cooperativa Central de Pesquisa Agrícola; Martins, Polyana Kelly; Cooperativa Central de Pesquisa Agrícola; Schuster, Ivan; Cooperativa Central de Pesquisa Agrícola.
The first step in plant transformation using somatic embryogenesis or organogenesis for plant regeneration in vitro has been the identification of genotypes with the best totipotency.  Thus, the objective of this experiment was to evaluate the totipotency of ten cultivars and one soybean line from the Coodetec Gene Bank. The somatic embryos developed from immature cotyledons measuring from 4 to 6 mm in length, and the organogenesis was evaluated from mature soybean seeds. The cultivars CD 201, CD 216 and CD 220 were the most promising genotypes to produce somatic embryos, whereas the cultivar CD 217 had the highest quantity of differentiated explants, which represents the best regeneration efficiency for participating in organogenic systems. In the...
Tipo: Laboratory analysis
Palavras-chave: Glycine max; Somatic embryogenesis; Organogenesis Glycine max; Somatic embryogenesis; Organogenesis Melhoramento Vegetal; Cultura de Tecidos.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12344

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg
Provedor de dados: 3
Autores: Rossato, Marieli; Schumacher, Pedro Vitor; Costa Netto, Antônio Paulino da; Stein, Vanessa Cristina; Reis, Edésio Fialho dos; Vilela, Marlúcia Souza Pádua; Paiva, Luciano Vilela.
Campomanesia adamantium is a native plant species of Brazilian Cerrado with diverse economic potential and great medicinal importance. Its sexual propagation is impaired by the recalcitrance of its seeds, which prevents effective and profitable propagation. With the purpose of establishing commercial crops and minimizing the extractive use of vegetal resources, the aim of the present study was to induce embryogenic calli in nodal segments of gabirobeira, and to determine and characterize their embryogenic phase through the establishment of a growth curve based on cellular characteristics. Calli were induced using nodal segments inoculated in WPM culture medium without the addition of hormones (control) and with different concentrations of 2,4-D, IAA, IBA,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Somatic embryogenesis; Growth regulator; Callus.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46358

Addition of benzyladenine to coconut explant cultured In vitro improves the formation of somatic embryos and their germination
Provedor de dados: 115
Autores: Montero-Cortés,Mayra I.; Chan-Rodríguez,José L.; Cordova-Lara,Ivan; Oropeza-Salin,Carlos; Sáenz-Carbonell,Luis.
Coconut (Cocos nucífera L.) production has been declining in México and other countries due to deadly diseases; therefore, massive production of palms resistant to diseases is needed for replanting. Production of somatic embryos and micropropagation could be a very useful technique to produce free disease coconut population. Explants (embryogenic-structures) of Pacific Tall 2 ecotype were cultured in an induction medium defined as I (semisolid Y3 medium containing 0.65 mM 2,4-dichlorophenoxyacetic acid) and treated with the cytokinin benzyladenine (BA) at 0, 25, 100 or 200 ,µM to evaluate its effect on embryogenic callus formation. The BA treatments did not affect the percentage of embryogenic callus formation. In order to evaluate the effect of the BA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocos nucífera; Clonal propagation; Somatic embryogenesis; Cytokinin.
Ano: 2011
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952011000600002

Induction of cassava somatic embryogenesis in liquid medium associated to floating membrane rafts
Provedor de dados: 52
Autores: Takahashi,Elizabete Keiko; Kobayashi,Adilson Kenji; Vieira,Luiz Gonzaga Esteves.
The objective of this study was to examine the effect of two culture systems, liquid medium associated to floating membranes and solid medium, both supplemented with different concentrations of 2,4-D, in the induction of somatic embryogenesis of cassava (Manihot esculenta Crantz). Only 28% of the young leaf lobes (with 9 µM 2,4-D) were induced to form organized embryogenic structures (OES) with membrane rafts, compared to 50% of the explants presenting this type of tissue in solid medium with 36 µM of 2,4-D. Despite the lower response observed in liquid medium with membrane, the amount of OES/explant in all 2,4-D concentrations was higher than solid medium. Based on the results and considering the high cost of the membrane rafts, this system was not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Somatic embryogenesis; Membrane rafts; Organized embryogenic structures (OES).
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100005

Biochemical and morphological changes during the growth kinetics of Araucaria angustifolia suspension cultures
Provedor de dados: 52
Autores: Santos,André Luis Wendt dos; Silveira,Vanildo; Steiner,Neusa; Maraschin,Marcelo; Guerra,Miguel Pedro.
Embryogenic cultures of Araucaria angustifolia were established in a BM liquid medium supplemented with 2 µM 2,4-dichlorophenoxyacetic acid, 1 µM 6-benzylaminopurine and 1 µM kinetin (BM2) and in a BM medium free of growth regulators (BM0). During 42 days in culture, the cell growth pattern of both cultures was similar. The pH of the culture medium of both BM0 and BM2 underwent progressive reduction during culture time. For both the embryogenic cultures a preferential uptake of glucose in the late stages of cell growth kinetics was observed. The extracellular protein content was similar for both the embryogenic cultures. Acetocarmine and Evan's blue double stain showed major differences for early somatic embryo organisation, in which only the embryogenic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucariaceae; Glucose; PH changes; Protein; Somatic embryogenesis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300001

Recurrent Somatic Embryogenesis and Plantlet Regeneration in Psidium guajava L.
Provedor de dados: 52
Autores: Bajpai,Anju; Kalim,Shahina; Chandra,Ramesh; Kamle,Madhu.
A simple and efficient protocol for recurrent somatic embryogenesis and plant regeneration is one of the prerequisites for genetic improvement of guava. An efficient reproducible regeneration somatic embryogenesis protocol was developed in four genotypes of Psidium guajava L. using immature zygotic embryo as starter explant. Somatic embryogenesis induction was obtained on MS basal medium supplemented with 2.0 mgL-1 2, 4-D, 400 mgL-1 L-glutamine, 6% sucrose and 500 mgL-1 Malt extract. Following SE induction different developmental stages of somatic embryos (Globular, heart-shaped, torpedo, cotyledonary) was directly obtained and further recurrent embryogenesis also obtained upon prolonged incubation in induction media. Addition of polyethylene glycol (50...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Somatic embryogenesis; Zygotic embryo; Recurrent; Maturation; Acclimatization; Induction; Regeneration.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100412

Anatomy of somatic embryogenesis in Carica papaya L.
Provedor de dados: 52
Autores: Fernando,Juliana A.; Melo,Murilo; Soares,Marli K. M.; Appezzato-da-Glória,Beatriz.
Mature zygotic embryos of Carica papaya L. ‘Sunrise Solo’ were used as explants for embryogenesis induction. The explants were inoculated on Murashige and Skoog culture medium supplemented with 2 mg.L-1 2,4-dichlorophenoxyacetic acid and incubated in darkness at 25+2°C. Histological analysis of callogenesis and somatic embryogenesis indicated occurrence of direct and indirect somatic embryogenesis development. Direct somatic embryo formation was observed from hypocotyledonary epidermic cells only from explant 18 days after inoculation. Somatic embryos formed indirectly were originated from embryogenic superficial cells of pre-embryonic complexes located on peripherical and on internal cell layers of callus 49 days after inoculation. Diverse morphological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Somatic embryogenesis; Papaya.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300005

Somatic embryogenesis in cassava genotypes from the northeast of Brazil
Provedor de dados: 52
Autores: Feitosa,Terezinha; Bastos,João Luíz Pinheiro; Ponte,Luíz Ferreira Aguiar; Jucá,Thiago Lustosa; Campos,Francisco de Assis de Paiva.
A method for the induction of somatic embryogenesis in eight cassava genotypes from northeastern Brazil is described. The explants used were shoot apexes isolated both from in vitro grown plants and from shoots that sprouted from stem cuttings. Somatic embryogenesis was achieved in high frequencies by the addition in the induction medium of the auxin picloram over a wide range of concentrations. Green cotyledons of primary somatic embryos were used as explants to induce somatic (cyclic) secondary embryogenesis in an inducing medium supplemented with picloram at 12 mg/L. The method could be used not only for the mass production of plants of the cassava genotypes, but also to generate explants (green cotyledons of somatic embryos) as themselves excellent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Cassava; Somatic embryogenesis; Plant tissue culture; Picloram.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200004

Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze)
Provedor de dados: 52
Autores: Santos,André Luis Wendt dos; Silveira,Vanildo; Steiner,Neusa; Vidor,Mário; Guerra,Miguel Pedro.
Embryogenic cultures of Araucaria angustifolia were induced from dominant and non-dominant zygotic embryos excised from immature seeds proceeding from three different genotypes and five harvest dates. Zygotic embryos were inoculated in inductive culture medium LP and BM supplemented with or without plant growth regulators 2,4-D (5 µM), BA (2 µM) and Kin (2 µM). The genotype of the mother tree and the developmental explant stage affected the induction frequency. In the maintenance phase, embryogenic cultures were maintained at continuous repetitive cell cycles every 20 days in semi-solid or liquid medium. In the maturation phase the culture medium was supplemented with different types and levels of growth regulators, osmotic agents, carbohydrates and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucaria angustifolia; Conifer; Somatic embryogenesis; Picea abies; Embryo maturation; Pinus sp.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100015

Efficient In Vitro Somatic Embryogenesis and Plant Regeneration from Mature and Immature Embryos of Wheat ( Triticum aestivum L.)
Provedor de dados: 52
Autores: Ahmadpour,Roghayeh; Zare,Nasser; Asghari-Zakarta,Rasool; Sheikhzadeh,Parisa.
ABSTRACT An efficient regeneration system is a pre-requisite for the application of genetic transformation and functional genomics study of important plants. In this study, the effect of different factors (plant growth regulators, casein hydrolysate, aspartic acid and ascorbic acid) on in vitro embryogenesis and regeneration of Arta, Bahar and Zagros cultivars from mature and immature explants were investigated. Immature and mature embryos were dissected from disinfected seeds 20-25 days after pollination and imbedded mature seeds, respectively, and cultured on MS (Murashige and Skoog) medium supplemented with different compounds. The results showed that immature embryos expose high capacity of embryogenesis and regeneration in comparison with mature...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Plant growth regulators; Somatic embryogenesis; Wheat.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100206

Effect of carbon source on morphology and histodifferentiation of Araucaria angustifolia embryogenic cultures
Provedor de dados: 52
Autores: Steiner,Neusa; Vieira,Felipe do Nascimento; Maldonado,Sara; Guerra,Miguel Pedro.
The aim of the present work was to establish in vitro conditions for the induction, stabilization and proliferation of embryogenic cultures of A. angustifolia. Pre-cotyledonary staged zygotic embryos inoculated BM medium supplemented with 5 µM 2,4- D, 2 µM BAP and Kin, and 3% maltose or sucrose resulted in 66.7% induction rate. The rate of induction of embryogenic cultures was affected by the carbon source, as well the multiplication and morphology of the embryogenic cultures. Embryogenic cultures maintained in BM medium with maltose presented bipolar morphology. Globular somatic embryos were obtained BM medium with 9% (PEG) and (9%) maltose. These results could establish an in vitro regenerative protocol towards the conservation and improvement of this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell competence; Somatic embryogenesis; Carbohydrate; Conifers.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800005

Coffee biotechnology
Provedor de dados: 61
Autores: De Los Santos-Briones,César; Hernández-Sotomayor,S. M. Teresa.
In the last three decades, interest has turned to in vitro cell culture in different areas of coffee research. In vitro techniques have been applied not only for coffee improvement through genetic transformation but also to study various aspects in coffee cells such as chemical (caffeine synthesis and the production of coffee aroma), physiological and more recently, biochemical aspects. The most important advances obtained to date on in vitro coffee techniques in fields like biochemistry, physiology, regeneration systems and genetic engineering, are presented and discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea; Coffee; Genetic transformation; In vitro tissue culture; Regeneration; Somatic embryogenesis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100015

Bioreactors in coffee micropropagation
Provedor de dados: 61
Autores: Etienne,Hervé; Dechamp,E; Barry-Etienne,D; Bertrand,Bernóit.
In coffee, bioreactors are the most promising way for scaling-up micropropagation processes, particularly somatic embryogenesis. The availability of an efficient somatic embryogenesis process would allow the rapid mass production of heterozygous materials such as selected Coffea canephora clones and F1 Arabica hybrid varieties. For the last fifteen years, bioreactors (mechanically or pneumatically agitated bioreactors, temporary immersion bioreactors) have mostly been used on coffee to optimize the mass regeneration of somatic embryos from embryogenic tissues. This review presents the main results, obtained with several bioreactor models, concerning the different steps of the micropropagation process : i) the multiplication of embryogenic tissues, ii) the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea; Breeding; Liquid medium; Mass propagation; Somatic embryogenesis; Temporary immersion.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100005

Encapsulated Somatic Embryos and Zygotic Embryos for Obtaining Artificial Seeds of Rauli-Beech (Nothofagus alpina (Poepp. &amp;amp; Endl.) Oerst.)
Provedor de dados: 117
Autores: Cartes R,Priscila; Castellanos B,Hermes; Ríos L,Darcy; Sáez C,Katia; Spierccolli H,Scarlette; Sánchez O,Manuel.
Somatic and zygotic embryos from mature seeds of rauli-beech, Nothofagus alpina (Poepp. &amp;amp; Endl.) Oerst., were encapsulated in different artificial endosperms in order to generate a cover that fulfills the function of nourishment and protection of the embryos, facilitating their later germination. The content of sodium alginate varied by 4%, 3%, and 2%, as did the immersion time in calcium chloride (CaCl2), which acts as complexing agent. The artificial endosperm components of the Murashige and Skoog medium (MS) were added, supplemented with 0.5 mg L-1 indolacetic acid (IAA), 0.5 mg L-1 naphthaleneacetic acid (NAA), 2 mg L-1 6-benzylaminopurine (BAP) and 30 g L-1 sucrose. The germinative behaviors of encapsulated somatic and zygotic embryos were...
Tipo: Journal article
Palavras-chave: Rauli-beech; Somatic embryogenesis; Synthetic seed; Sodium alginate.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000100014

Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. &amp;amp; Endl.) Oerst
Provedor de dados: 117
Autores: Cartes Riquelme,Pricila; Ríos Leal,Darcy; Sáez Carrillo,Kátia; Uribe Moraga,Matilde; Valenzuela Aguilar,Sofía; Joseph Bolus,Stephen; Sánchez Olate,Manuel.
Abscisic acid (ABA) and indole-3-acetic acid (IAA) participate in the propagation of plants by somatic embryogenesis, causing polar structural differentiation of the embryo. The goal of the assay was to compare endogenous levels of ABA and IAA between somatic embryos (SE) and zygotic embryos (ZE) of Nothofagus alpina (Poepp. &amp;amp; Endl.) Oerst. In this study, a somatic embryo maturation assay involving the addition of varying concentrations of exogenous ABA was performed on cotyledonary-stage of N. alpina. Furthermore, the endogenous levels of ABA and IAA were quantified in the immature ZE, the mature ZE, and the embryonic axis of a mature embryo of N. alpina. The current study utilized high performance liquid chromatography (HPLC) for quantification....
Tipo: Journal article
Palavras-chave: Somatic embryogenesis; Maturation; Germination; HPLC.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000400007

Plant tissue culture: Current status, opportunities and challenges
Provedor de dados: 118
Autores: García-Gonzáles,Rolando; Quiroz,Karla; Carrasco,Basilio; Caligari,Peter.
In the last two decades plant biotechnology applications have been widely developed and incorporated into the agricultural systems of many countries worldwide. Tissue culture tools have been a key factor to support such outcomes. Current results have allowed plant biotechnology and its products -including transgenic plants with several traits- to be the most assimilated technology for farmers and companies, representing several benefits such as: 125 millions ha of transgenic crops in 2008, the reduction of pesticides application by up to 9% in the last ten years, transgenic plants with a better nutritional quality, mass propagation of selected and healthy plants, and the production of proteins for industrial or therapeutic use. The rapid and extensive...
Tipo: Journal article
Palavras-chave: In vitro; Micropropagation; Organogenesis; Plant bioreactors; Somatic embryogenesis; Transgenic plants.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000300001

Somatic embryos from Phalaenopsis classic Spotted Pink (Orchidaceae) protocorms
Provedor de dados: 65
Autores: Pereira,João Alves Ferreira; Ferreira,Laís Tomaz; Morais,Marciana Bizerra de; Ulisses,Cláudia.
ABSTRACT:  The aim of the present study was to induce the formation of somatic embryos in protocorms from Phalaenopsis Classic Spotted Pink hybrids at two physiological maturation stages, namely: 80 and 120 days after seed inoculation (DASI). Protocorms were inoculated in ½ MS medium supplemented with 0.1 mg L-1 ANA and 3 mg L-1 TDZ. Protocorms were inoculated 120 days after sowing were more developed at the 15th cultivation day due to the formation of pro-embryogenic structures. It was possible seeing the formation of globular- and torpedo-stage somatic embryos at the 30th day of cultivation in somatic embryogenesis (SE) induction medium. The protocorms inoculated at the 80th DASI did not formed somatic embryos; they oxidized 20 days after cultivation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orchid; Protocormoids; Somatic embryogenesis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700401

Date palm micropropagation: Advances and applications
Provedor de dados: 64
Autores: Al-Khayri,Jameel Mohammed; Naik,Poornananda Madhava.
ABSTRACT Date palm (Phoenix dactylifera L.) is a fruit tree resilient to adverse climatic conditions predominating in hot arid regions of the Middle East and North Africa. The date fruit contains numerous chemical components that possess high nutritional and medicinal values. Traditional propagation by offshoots is inefficient to satisfy current demands for date palm trees. Alternatively, micropropagation provides an efficient means for large-scale propagation of date palm cultivars. Both somatic embryogenesis and organogenesis, either directly or indirectly though the callus phase, have been demonstrated in date palm in vitro regeneration. Culture initiation commonly utilizes shoot-tip explants isolated from young offshoots. Recently, the immature...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Somatic embryogenesis; Somaclonal variation; Organogenesis; Molecular marker.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542017000400347