Consulta: subjectFacets:"Streptococcus pneumoniae"
Registros recuperados: 26
Data/hora: 10/06/2024 13:23:13

Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14
Provedor de dados: 69
Autores: Moura Leal,Maria de Lourdes; da Silva Gomes Pereira,Denise; Jessouroun,Ellen; Peixoto Gimenes Couto,Maria Antonieta; Pereira Jr,Nei.
Streptococcus pneumoniae (pneumococcus) is among the most significant causes of bacterial disease in humans. Capsular polysaccharide (CPS) production is essential for pneumococcal virulence. Pneumococcal CPS has been widely used as vaccine antigen. This study is focused on the influence of culture conditions of Streptococcus pneumoniae serotype 14 as for developing an industrial method for polysaccharide production. The pH proved to be a highly important variable in batchwise culture. Using the pH control all glucose added was consumed resulting in a four-fold increase in polysaccharide productivity relative to cultivation without pH control. S. pneumoniae is a lactic acid bacterium, so named for its primary metabolic byproduct (lactate), which has an...
Tipo: Journal article
Palavras-chave: Polysaccharide capsular; Vaccine; Streptococcus pneumoniae.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500006

Viviendo treinta años con el enemigo: neumococos resistentes a los antibióticos en la Argentina
Provedor de dados: 38
Autores: Lopardo,Horacio Ángel; Fossati,Sofía.
En la Argentina, los primeros neumococos no sensibles a penicilina (PNSP) se detectaron en 1981 en un hospital pediátrico de la ciudad de La Plata. Interesantemente, no se diseminaron a otros centros sino hasta 7 años después. Luego aumentaron desde un 17% en 1994 hasta un 43,2% en 1996, y permanecieron en esas cifras hasta la actualidad. Se observaron también cambios en la prevalencia de neumococos resistentes a penicilina (PRSP) (CIM&amp;#8805;2 &amp;#956;g/mL) con picos a fines de la década del 90 y una reducción importante en los últimos años. Se supone que esta frecuencia decreciente de los PRSP pudo obedecer a cambios en la prevalencia de determinados clones cuyas alteraciones en las PBP no hubieran sido tan importantes como para comprometer su...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Resistencia; Antibióticos.
Ano: 2016
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572016000400019

Autolisis de Streptococcus pneumoniae en hemocultivos
Provedor de dados: 38
Autores: Villar,Hugo Edgardo; Longo,Liliana María; Laurino,Gustavo Jorge; Vicente,Adriana; Gutiérrez,Marisa Estela; Hoffman,Marta.
El proceso de autolisis de Streptococcus pneumoniae en medios líquidos puede ocasionar problemas en muestras de hemocultivos impidiendo su recuperación y, por lo tanto, el diagnóstico microbiológico, así como el estudio de sensibilidad a los antimicrobianos. El objetivo de este trabajo fue revisar la frecuencia de este fenómeno en muestras clínicas y determinar las mejores condiciones de detección para este germen en los sistemas automatizados. Fueron incluidos todos los pacientes con diagnóstico de neumonía y hemocultivos positivos por S. pneumoniae y pacientes con diagnóstico de neumonía, botellas positivas y subcultivos negativos. Se evaluó el tipo de botella empleada, tiempo de detección, tiempo de demora en subcultivar las botellas y recuperación del...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Autolisis; Hemocultivos; BacT/Alert.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572005000200002

Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical samples
Provedor de dados: 55
Autores: de Filippis,Ivano; Andrade,Claudia Ferreira de; Caldeira,Nathalia; Azevedo,Aline Carvalho de; Almeida,Antonio Eugenio de.
Abstract  Background Several in-house PCR-based assays have been described for the detection of bacterial meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae from clinical samples. PCR-based methods targeting different bacterial genes are frequently used by different laboratories worldwide, but no standard method has ever been established. The aim of our study was to compare different in-house and a commercial PCR-based tests for the detection of bacterial pathogens causing meningitis and invasive disease in humans.  Methods A total of 110 isolates and 134 clinical samples (99 cerebrospinal fluid and 35 blood samples) collected from suspected cases of invasive disease were analyzed. Specific sets of primers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnostic; Neisseria meningitidis; Streptococcus pneumoniae; Haemophilus influenzae.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400335

Clinical and bacteriological characteristics of invasive pneumococcal disease after pneumococcal 10-valent conjugate vaccine implementation in Salvador, Brazil
Provedor de dados: 55
Autores: Leite,Carolina Regis; Azevedo,Jailton; Galvão,Vivian Santos; Moreno-Carvalho,Otávio; Reis,Joice Neves; Nascimento-Carvalho,Cristiana.
Abstract Invasive pneumococcal disease is a relevant public health problem in Brazil, especially among children and the elderly. In July/2010 a 10-valent pneumococcal conjugate vaccine was introduced to the immunization schedule of Brazilian children under two years of age. Between July/2010 and December/2013 we conducted a case-series study on invasive pneumococcal disease in Salvador, Brazil to describe the clinical and bacteriological profile of invasive pneumococcal disease cases during the post-implementation period. Eighty-two cases were eligible. Mean age was 31 years (interquartile range, 3–42); 17.1% and 30.5% were under 2 years and 5 years, respectively. Pneumococcal meningitis (n = 64, 78.1%), bacteraemic pneumococcal pneumonia (n = 12, 14.6%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Vaccine; Epidemiology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000100056

Susceptibility of Streptococcus pneumoniae to penicillin in the state of Minas Gerais, Brazil from 1997-2004
Provedor de dados: 55
Autores: Bedran,Maria B. M.; Camargos,Paulo A. M.; Leocádio Filho,Geraldo; Bedran,Renata M.; Najar,Henrique C.
We monitored the susceptibility to penicillin of invasive strains of Streptococcus pneumoniae in two reference laboratories; 502 positive cultures from patients with an active invasive infectious process were analyzed. Streptococcus pneumoniae was identified through conventional procedures, and the oxacillin disc diffusion method was used to check for penicillin susceptibility. Statistical analysis included calculations of the frequency distribution, with 95% confidence intervals (CI), as well as chi-square tests and chi-square for linear trend for temporal analysis of susceptibility. The bacterium was isolated from patients less than a year old (40.7% of the isolates), from infants (55.9%), and from individuals less than 15 years old (64.4%). The majority...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Antimicrobial resistance; Invasive disease.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000500006

Antibacterial resistance of community-acquired respiratory tract pathogens recovered from patients in Latin America: results from the PROTEKT surveillance study (1999-2000)
Provedor de dados: 55
Autores: Mendes,C.; Marin,M. E.; Quiñones,F.; Sifuentes-Osornio,J.; Cuilty Siller,C.; Castanheira,M.; Zoccoli,C. M.; López,H.; Súcari,A.; Rossi,F.; Barriga Angulo,G.; Segura,A.J.A.; Starling,C.; Mimica,I.; Felmingham,D.
PROTEKT (Prospective Resistant Organism Tracking and Epidemiology for the Ketolide Telithromycin) is a global surveillance study established in 1999 to monitor antibacterial resistance of respiratory tract organisms. Thirteen centers from Argentina, Brazil and Mexico participat ed during 1999-2000; they collected 1,806 isolates (Streptococcus pneumoniae 518, Haemophilus influenzae 520, Moraxella catarrhalis 140, Staphylococcus aureus 351, S. pyogenes 277). Overall, 218 (42.1%) of the S. pneumoniae isolates had reduced susceptibility to penicillin, 79 (15.3%) were penicillin-resistant and 79 (15.3%) were erythromycin-resistant. Mexico had the highest prevalence of penicillin (76.5%) and erythromycin (31.2%) resistance. Of 77 erythromycin-resistant S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PROTEKT; Latin America; Resistance; Respiratory tract infections; Streptococcus pneumoniae; Haemophilus influenzae; Moraxella catarrhalis; Streptococcus pyogenes; Staphylococcus aureus.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000100006

Prevalence of pneumococcal serotypes and resistance to antimicrobial agents in patients with meningitis: ten-year analysis
Provedor de dados: 55
Autores: Alvares,Jackelline Rodrigues; Mantese,Orlando Cesar; Paula,Alan de; Wolkers,Paula Carolina Bejo; Almeida,Viviene Vieira Prado; Almeida,Samanta Cristine Grassi; Guerra,Maria Luiza Leopoldo Silva; Brandileone,Maria Cristina de Cunto.
OBJECTIVES: To determine the prevalence of pneumococcal serotypes and antimicrobial susceptibility in patients with meningitis, and to evaluate the implications for vaccine coverage. METHODS: Pneumococcal strains obtained from normally sterile fluids from patients admitted with meningitis were isolated at the Hospital de Clínicas of the Universidade Federal de Uberlândia, Minas Gerais State, and sent to the Instituto Adolfo Lutz, city of São Paulo, São Paulo State, for further identification, serotyping, and antimicrobial susceptibility determination. RESULTS: From April 1999 to April 2009, 338 pneumococcal strains were isolated, and 72 obtained from patients with meningitis, were analyzed. Patients' ages varied from one month to 82.2 years (mean of 18.4 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Meningitis; Serotyping; Pneumococcal vaccines; Drug resistance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100005

Antimicrobial resistance in respiratory pathogens isolated in Brazil during 1999-2000
Provedor de dados: 55
Autores: Critchley,Ian A.; Blosser,Renée S.; Karlowsky,James A.; Yamakita,Juri; Barth,Alfonso; Sader,Helio S.; Mendes,Caio; Teixeira,Lucia; Rossi,Flavia; Dias,Cicero A. C.; Jones,Mark E.; Thornsberry,Clyde; Sahm,Daniel F.
The in vitro antimicrobial susceptibility of the respiratory pathogens Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis to commonly tested and prescribed agents was investigated during 1999-2000 and compared with results obtained during a previous 1997-1998 study. Of 448 isolates of S. pneumoniae collected and tested in 1999-2000, 77.2% were susceptible, 19.9% were intermediate, and 2.9% were resistant to penicillin, demonstrating that there were no major changes in susceptibility to penicillin from 1997-1998 (77.1% susceptible, 18.7% intermediate, 4.2% resistant). All S. pneumoniae isolates from 1999-2000 were susceptible to levofloxacin and vancomycin, and &gt;90% were susceptible to the beta-lactams (amoxicillin-clavulanate,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Antimicrobial resistance; Surveillance study; Respiratory pathogen.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000600002

Nasopharyngeal colonization and penicillin resistance among pneumococcal strains: a Worldwide 2004 update
Provedor de dados: 55
Autores: Cardozo,Déa M.; Nascimento-Carvalho,Cristiana M. C.; Souza,Fabiane R.; Silva,Nívea M. S.
Surveillance of nasopharyngeal pneumococcus has proven to be a valuable tool for the monitoring of antibiotic resistance. We reviewed the latest information on colonization rate and penicillin resistance by making a MEDLINE search, using the terms "nasopharyngeal carriage" and "Streptococcus pneumoniae". Out of 225 articles found, data from 109 recent publications (89% from 1996-2003) were analyzed. Data were reported from 41 countries of six continents. Individuals under the age of five (64.3%) or 10 years (85.7%) were enrolled, including children attending day-care centers (32.1%) or orphanages (3.6%), and healthy individuals (78.6%) or sick patients (43.6%); biological samples were collected mainly by nasopharyngeal swabs (89.3%). The highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Nasopharyngeal carriage; Penicillin resistance.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000400015

Clinical and microbiological implications of invasive pneumococcal disease in hospitalized patients (1998–2013)
Provedor de dados: 55
Autores: Medeiros,Marta Inês Cazentini; Negrini,Bento Vidal de Moura; Silva,Jorgete Maria e; Almeida,Samanta Cristine Grassi; Guerra,Maria Luiza Leopoldo Silva; Andrade,Denise de.
Abstract  Introduction Infections caused by Streptococcus pneumoniae (pneumococcus) still represent a challenge for health systems around the world.  Objective The objective of this study was to assess microbiological and clinical aspects in hospitalized patients with invasive pneumococcus disease between 1998 and 2013.  Materials and methods This was a retrospective study that analyzed the results of pneumococcus identification, serotyping, and susceptibility testing found in the Adolfo Lutz Institute databank. Personal variables, medical history and clinical outcome of patients admitted with invasive pneumococcal disease were analyzed. These were obtained from records of a public teaching hospital – Hospital das Clínicas Faculdade de Medicina Ribeirão...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Serotypes; Death; Comorbidity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000300004

Community acquired pneumonia among pediatric outpatients in Salvador, Northeast Brazil, with emphasis on the role of pneumococcus
Provedor de dados: 55
Autores: Nascimento-Carvalho,Cristiana M.C.; Lopes,Antônio A.; Gomes,Maria Daniela B.S.; Magalhães,Morgana P.; Oliveira,Juliana R.; Vilas-Boas,Ana Luisa; Ferracuti,Roberta; Brandileone,Maria Cristina C.; Guerra,Maria Luiza L.S.; Alves,Noraney N.; Athayde,Ledilce A.; Caldas,Renilza M.; Barberino,Maria Goreth M. A.; Duarte,Jussara; Brandão,Maria Angélica S.; Rocha,Heonir; Benguigui,Yehuda; Di Fabio,José Luis.
Pneumonia is one of the leading causes of hospitalization and death among children in developing countries, and mortality due to pneumonia has been associated with S. pneumoniae infection. This investigation was designed to describe the antimicrobial susceptibility and serotype patterns of pneumococcal strains recovered from the blood of children with community-acquired pneumonia (CAP) and to assess the clinical findings of pneumococcal bacteremic patients with pneumonia. In a 26 month prospective study, blood cultures were obtained as often as possible from children (&lt;16 years of age) diagnosed with CAP in two emergency rooms. Antimicrobial drug susceptibility tests and serotyping were performed when pneumococcus was identified. We studied 3,431 cases...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Antimicrobial resistance; Serotypes; Blood culture; Community-acquired pneumonia; Children.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000100003

Bacteremic pneumococcal pneumonia: serotype distribution, antimicrobial susceptibility, severity scores, risk factors, and mortality in a single center in Chile
Provedor de dados: 55
Autores: Fica,Alberto; Bunster,Nicolás; Aliaga,Felipe; Olivares,Felipe; Porte,Lorena; Braun,Stephanie; Dabanch,Jeannette; Hormázabal,Juan Carlos; Hernández,Antonio; Benavides,María Guacolda.
AIMS:Bacteremic pneumococcal pneumonia (BPP) is a severe condition. To evaluate seasonal distribution, mortality, serotype frequencies, antimicrobial susceptibility, and different severity scores among patients with BPP.PATIENTS AND METHODS:Patients were identified by laboratory data and restricted to adulthood. Standard methods were used for serotyping and antimicrobial susceptibility. Risk factors were analyzed by univariate and multivariate methods. Severity scores (APACHE II, CURB-65 and CAP PIRO) were compared using ROC curves.RESULTS:Sixty events of community-acquired BPP occurred between 2005 and 2010. A seasonal pattern was detected. Mean age was 72.1 years old (81.4% &gt;60 years). All had a predisposing factor. Previous influenza (3.3%) or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Bacteremia; Serotyping; Microbial drug resistance; Risk factors; Mortality; Elderly; CURB-65; APACHE II; PIRO.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000200115

Evaluation of the incidence of occult bacteremia among children with fever of unknown origin
Provedor de dados: 55
Autores: Berezin,Eitan Naaman; Iazzetti,Marco Antonio.
We reviewed the incidence of occult bacteremia, to identify the most frequent etiological agents of bacteremias in otherwise healthy children from one month to 10 years old, who had fever of unknown origin attended at the emergency ward of an urban, university-affiliated pediatric referral center. This was a retrospective medical record review, evaluating children with fever. Data were collected from the initial visit, when blood cultures, hematological properties and hemosedimentation rates were examined. Fever was considered as the highest temperature assessed in the hospital or reported by the responsible adult. Occult bacteremia was discovered in 1.4% of the 1,051 children evaluated, and the most common etiologic agent was Streptococcus pneumoniae....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fever; Streptococcus pneumoniae; Occult bacteremia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600007

Serotype distribution and antimicrobial resistance of Streptococcus pneumoniae causing noninvasive diseases in a Children's Hospital, Shanghai
Provedor de dados: 55
Autores: Pan,Fen; Han,Lizhong; Kong,Jing; Wang,Chun; Qin,Huihong; Xiao,Shuzhen; Zhu,Junying; Zhang,Hong.
Background: Streptococcus pneumoni ae, which cause noninvasive pneumococcal diseases, severely impair children's health. This study analyzed serotype distribution and antimicrobial resistance of S. pneumoni ae from January 2012 to December 2012 in a Children's Hospital, Shanghai.  Methods: A total of 328 pneumococcal isolates were serotyped by multiplex sequential PCR and/or capsule-quellung reaction. The minimum inhibitory concentrations for 11 antimi- crobial agents were determined by broth microdilution method.  Results: Among 328 strains, 19F (36.3%), 19A (13.4%), 6A (11.9%), 23F (11.0%), 14 (5.8%), 6B (5.2%), and 15B/C (4.3%) were the most common serotypes. The coverage rates of 7-, 10-, and 13-valent conjugate vaccines (PCV7, PCV10, and PCV13) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Serotype; Antimicrobial resistance.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200141

Immunogenicity of a 23-valent pneumococcal polysaccharide vaccine in elderly residents of a long-term care facility
Provedor de dados: 55
Autores: Valenzuela B.,M. Teresa; Altuzarra H.,Rogelio; Trucco A.,Olivia; Villegas R.,Rodrigo; Inostroza S.,Jaime; Granata S.,Paulo; Fleiderman V.,José; Maggi C.,Leonardo.
S. pneumoniae is a significant cause of community-acquired pneumonia in the elderly, and accounts for the majority of the pneumonia deaths among the elderly. We conducted this randomized double-blind study to evaluate the immune response to a 23-valent pneumococcal polysaccharide vaccine and the persistence of antibodies two years after the vaccination in an elderly population in Santiago, Chile. A total of 118 elderly nursing home residents received either the pneumococcal or a tetanus control vaccine. Serum samples were taken at enrolment, at two months, and at two years post-vaccination. Pre-vaccination anti-pneumococcal antibody geometric mean concentrations (GMC) were similar in both study groups, with increased levels of antibodies found only against...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Vaccine; Elderly; Immunogenicity; Antibodies.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300005

Serotype changes and antimicrobial nonsusceptibility rates of invasive and non-invasive Streptococcus pneumoniae isolates after implementation of 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) in Bulgaria
Provedor de dados: 55
Autores: Setchanova,Lena; Murdjeva,Marianna; Stancheva,Iglika; Alexandrova,Alexandra; Sredkova,Maria; Stoeva,Temenuga; Yoneva,Magda; Kurchatova,Anna; Mitov,Ivan.
Abstract The 10-valent pneumococcal conjugate vaccine (PCV10) has been included in Bulgarian Childhood Immunization Program since 2010. This study aimed to assess serotype distribution and antimicrobial resistance of 198 invasive and non-invasive Streptococcus pneumoniae strains that had been isolated in Bulgaria during 2011-2016 from patients with invasive (IPD) and non-invasive (NIPD) pneumococcal diseases. The most common invasive serotypes were 3 (10.1%), 19F (4.0%), and 7F (3.0%). A significant decrease in the proportion of invasive vaccine types (VTs) from 64.2% to 35.2% was found in comparison with pre-vaccine era. The most common serotypes among middle ear fluids were 3, 19A and 19F (5.6% each), and VTs fell down from 66.4% to 40.0% in post-PCV10...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Invasive and non-invasive isolates; Serotypes; Antimicrobial nonsusceptibility.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400433

Rapid diagnosis of community-acquired pneumonia using the Bac T/ alert 3D system
Provedor de dados: 55
Autores: Capoor,Malini R.; Nair,Deepthi; Aggarwal,Pushpa; Gupta,B.
We compared BacT/Alert 3D with conventional culture for the diagnosis of community-acquired pneumonia (CAP). Antimicrobial susceptibility testing of the isolates was performed with the disk diffusion method, and the minimum inhibitory concentration (MIC) was calculated. Automation was superior in terms of recovery and time to detect pathogens. The bacterial spectrum in CAP was Streptococcus pneumoniae (35.3%) Staphylococcus aureus (23.5%), Klebsiella pneumoniae (20.5%) and Haemophilus influenzae (8.8%). Three of the 12 S. pneumoniae isolates showed penicillin resistance on MIC and two showed erythromycin resistance. There were two H. influenzae strains resistant to penicillin; these were beta lactamase producers. One-fourth of the S. aureus were oxacillin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Community-acquired pneumonia; Streptococcus pneumoniae; Cefepime.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500010

Community-acquired pneumonia in the childhood: analysis of the diagnostic methods
Provedor de dados: 55
Autores: Requejo,Henry I. Z.
Immunological assays such as CIE, LA, and Dot-ELISA were compared in order to diagnose community-acquired pneumonia. Serum, pleural fluid and urine samples were comparatively employed for bacterial antigen detection. Dot-ELISA proved to be an original and practical alternative procedure for detecting bacterial polysaccharide antigens from pleural fluid and/or concentrated urine samples, providing a rapid diagnosis for pediatric patients with community-acquired pneumonia.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Community-acquired pneumonia; Streptococcus pneumoniae; Haemophilus influenzae; Bacterial pneumonia; Immunological methods.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200016

Factors associated with penicillin-nonsusceptible pneumococcal infections in Brazil
Provedor de dados: 56
Autores: Levin,A.S.; Sessegolo,J.F.; Teixeira,L.M.; Barone,A.A.
Resistance of Streptococcus pneumoniae is a worldwide, growing problem. Studies of factors associated with resistance to penicillin have not been conducted in Brazil. The objective of the present study was to evaluate factors associated with infection by S. pneumoniae not susceptible to penicillin. A prevalence study was conducted including all patients with a positive culture for S. pneumoniae in a hospital from July 1991 to December 1992 and the year 1994. Of 165 patients identified, 139 were considered to have clinically relevant infections and 88% of them had invasive infections. All infections were community acquired and consisted of pneumonia (44%) and of central nervous system (19%), pelvic or abdominal (12%), upper airway or ocular (12%), primary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Penicillin susceptibility; Risk factors; Prevalence study.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600017

Immunogenicity of a 23-valent pneumococcal polysaccharide vaccine in Brazilian elderly
Provedor de dados: 56
Autores: Simonsen,V.; Brandão,A.P.; Brandileone,M.C.C.; Yara,T.I.; Di Fabio,J.L.; Lopes,M.H.; Jacob Filho,W.
Serum antibodies specific for the capsular polysaccharides of Streptococcus pneumoniae provide protection against invasive pneumococcal infection. In Brazil, this vaccine has been used for people over 65 years with clinical risk to develop pneumococcal infection since 1999. We evaluated the immune response of 102 elderly subjects (75.5% females and 24.5% males) with a mean age of 71 years, and 19 young healthy adults (63.2% females and 36.8% males) with a mean age of 27 years. The elderly study group consisted of outpatients who received follow-up care in the Geriatric Department of General Hospital, Faculty of Medicine, University of São Paulo. None had acute illness at the time of vaccination. Both groups were immunized with one intra-deltoid injection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Vaccine; Pneumococcal polysaccharide vaccine; Immune response; Elderly subjects.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200014

Antibody response to pneumococcal capsular polysaccharide vaccine in Down syndrome patients
Provedor de dados: 56
Autores: Costa-Carvalho,B.T.; Martinez,R.M.A.; Dias,A.T.N.; Kubo,C.A.; Barros-Nunes,P.; Leiva,L.; Solé,D.; Carneiro-Sampaio,M.M.S.; Naspitz,C.K.; Sorensen,R.U.
The majority of children with Down syndrome (DS) tend to have frequent bacterial infections including recurrent respiratory infections. Our objective was to evaluate the production of antibodies to pneumococcal polysaccharide antigens after active immunization in DS subjects. IgG antibodies to pneumococcal serotypes (1, 3, 6B, 9V, and 14) were measured before and 6 weeks after immunization with a 23-valent pneumococcal vaccine (Pneumo23®, Pasteur-Merrieux) in 6- to 13-year-old DS children (N = 17) and in aged-matched normal controls (N = 30). An adequate response was defined as a 4-fold increase over baseline or a post-immunization level of specific pneumococcal serotype antibody  &gt; or = 1.3 µg/mL. After immunization, all DS children had an increase in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Down syndrome; Immunoglobulin; IgG subclass; Polysaccharide vaccine; Streptococcus pneumoniae; Pneumococcal antibodies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200010

Antimicrobial susceptibility of Streptococcus pneumoniae and genotypic characterization of erythromycin-resistant strains in Porto Alegre, Brazil
Provedor de dados: 58
Autores: Weber,F. T.; Dias,C.; Costa,M. da.
The antimicrobial susceptibility of 64 strains of S. pneumoniae obtained from three hospitals in Porto Alegre, Brazil, isolated between 2004 and 2005, was determined, using the agar-dilution method. The prevalence of resistant (intermediate and full resistance) strains to trimethoprim/sulphamethoxazole, penicillin, tetracycline, erythromycin, chloramphenicol, and ceftriaxone were 68%, 28%, 18%, 15%, 3%, and 1%, respectively. All strains were susceptible to vancomycin. Among 18 penicillin-resistant strains, 7 were resistant to at least two other antimicrobial drugs. All erythromycin-resistant strains, except one, contained the erm(B) and/or mef(A/E) genes, with a predominance of the former. The resistance rate to penicillin and erythromycin in Porto Alegre...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Drug resistance; Erm(B); Mef(A/E).
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100001

The virulence of Streptococcus pneumoniae partially depends on dprA
Provedor de dados: 58
Autores: Yu,Yi; Chang,De; Xu,Huiwen; Zhang,Xuelin; Pan,Lei; Xu,Chou; Huang,Bing; Zhou,Hong; Li,Jia; Guo,Jun; Liu,Changting.
Abstract Streptococcus pneumoniae is one of the most frequent opportunistic pathogens worldwide. DNA processing protein A (DprA) is an important factor involved in bacterial uptake and DNA integration into bacterial genome, but its role in S. pneumoniae virulence remains unclear. The aim of this study was to characterize the effects of the pneumococcal dprA gene on the pathogenesis of S. pneumoniae. To construct a dprA-deficient pneumococcal strain, the dprA gene of the S. pneumoniae strain D39 was inactivated. The virulence of this dprA-deficient strain, designated ΔD39, was compared with that of the wild-type strain by evaluating their respective capabilities to adhere to human pulmonary epithelial cells (PEC-A549) and by analyzing their choline-binding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; DprA; Transformation; Virulence.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200225

Antimicrobial susceptibility and fluctuations in clonal complexes of serogroup 6 Streptococcus pneumoniae isolates collected from children in Beijing, China, between 1997 and 2016
Provedor de dados: 58
Autores: Shi,Wei; Liu,Ying; Meng,Qinghong; Yuan,Lin; Gao,Wei; Yao,Kaihu.
ABSTRACT This study examined the antimicrobial susceptibility patterns and clonal complex (CC) characteristics of serogroup 6 Streptococcus pneumoniae isolates collected from children in Beijing, China, between 1997 and 2016. Serotypes were determined using the Quellung reaction, and the antimicrobial susceptibility profiles of the isolates were determined using the disc-diffusion method or by E-test. Sequence types (STs) were assigned based on multilocus sequence typing. A total of 250 isolates were examined, with 55.2%, 30.0%, 12.8%, and 2.0% of isolates identified as serotypes 6A, 6B, 6C, and 6D, respectively. All of the isolates were susceptible to levofloxacin and vancomycin, and the non-suceptibitility rate to penicillin was 41.6%. Eighty-two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Children; Antimicrobial susceptibility; Clonal complex (CC); Sequence type (ST).
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400891

Antimicrobial resistance and serotyping of Streptococcus pneumoniae isolated from pediatric patients in Belo Horizonte, MG, Brazil
Provedor de dados: 58
Autores: Magalhães,Ana Paula Gomes de Oliveira; Pinto,Artur da Silveira.
Thirty one Streptococcus pneumoniae invasive strains were isolated from a pediatric population in Belo Horizonte from June, 1999 to May, 2001. Penicillin, trimethoprim-sulfamethoxazole, tetracycline and chloramphenicol resistance rates for the isolates were 41.9, 58.1, 25.8 and 3.2%, respectively. Intermediate penicillin resistant (MICs between 0.1 and 1.0 µg/ml) and resistant (MICs &gt; 2.0 µg/ml) isolates occured at rates of 38.7 and 3.2%, respectively. Resistance to erythromycin, ofloxacin, rifampin or vancomicyn was not detected. Ten S. pneumoniae serotypes (14, 5, 10 A, 6B, 15B, 18C, 6 A, 18 A, 19 A and 19 F) were identified. Serotype 14 (12 out of 31) was predominant among the isolates. Penicillin and trimethoprim-sulfamethoxazole resistance was more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Antimicrobial resistance; Serotyping.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300005