Consulta: subjectFacets:"T-RFLP"
Registros recuperados: 4
Data/hora: 13/06/2024 02:34:06

Diversidade genética da comunidade de microrganismos da rizosfera de genótipos de milho e sorgo cultivados em condições de campo sob diferentes fontes e níveis de fósforo.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: CAMPOLINO, M. L.; LANA, U. G. de P.; GOMES, E. A.; COELHO, A. M.; SOUSA, S. M. de.
Os fertilizantes químicos têm sido fundamentais para a intensificação da agricultura. Entretanto, se aplicados em excesso, podem contaminar o meio ambiente, além de aumentarem significativamente os custos de produção. Somente os cereais recebem quase metade das aplicações de fertilizantes fosfatados no mundo. Sendo assim, a eficiência de uso do fósforo (P) pelas plantas deve ser aumentada, visando uma agricultura mais sustentável. Uma alternativa consiste na utilização de fontes menos solúveis de P associadas a genótipos mais eficientes. Além disso, a simbiose entre fungos micorrízicos arbusculares (FMA) e/ou bactérias solubilizadoras de P e plantas pode contribuir para aumentar a aquisição desse nutriente e promover o crescimento das plantas cultivadas....
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Comunidade bacteriana; T-RFLP; Fungo; Bactéria; Genótipo; Biologia do Solo.
Ano: 2018
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1099995

Bacterial community structure along the Adour estuary (French Atlantic coast): influence of salinity gradient versus metal contamination
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Goni-urriza, M. S.; Point, D.; Amouroux, D.; Guyoneaud, R.; Donard, O. F. X.; Caumette, P.; Duran, R.
Salinity, other physico-chemical parameters and anthropogenic pollution are the main factors affecting bacterial communities in estuaries. We estimated the impact of these parameters on the distribution of bacterial communities in the Adour estuary (France), a moderately Polluted water body characterized by short residence times of particles and water and absence of a maximum turbidity zone. Eight stations were established along the salinity gradient from freshwater to marine conditions. For the 3 typical estuarine stations (water mixing zone), samples were collected at both low and high tide and at different depths according to the position of the halocline. This sampling strategy generated 35 samples with different degrees of mixing between fresh water...
Tipo: Text
Palavras-chave: Bacterial communities; Salinity gradient; Heavy metals; Estuary; Halocline; T-RFLP.
Ano: 2007
URL: https://archimer.ifremer.fr/doc/00249/35983/34517.pdf

Diversity of the marine picocyanobacteria Prochlorococcus and Synechococcus assessed by terminal restriction fragment length polymorphisms of 16S-23S rRNA internal transcribed spacer sequences
Provedor de dados: Revista Chilena de Historia Natural
Autores: LAVIN,PARIS; GÓMEZ,PATRICIA; GONZÁLE,BERNARDO; ULLOA,OSVALDO.
In order to assess the appropriateness of the use of internal transcribed spacer (ITS) sequences for the study of population genetics of marine cyanobacteria, we amplified and cloned the 16S rRNA gene plus the 16S-23S ITS regions of six strains of Prochlorococcus and Synechococcus. We analyzed them by denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphisms (T-RFLP). When using the standard application of these techniques, we obtained more than one band or terminal restriction fragment (T-RF) per strain or cloned sequence. Reports in literature have suggested that these anomalies can result from the formation of secondary structures. Secondary structures of the ITS sequences of Prochlorococcus and Synechococcus...
Tipo: Journal article
Palavras-chave: Cyanobacteria; DGGE; T-RFLP; ITS; Secondary structure.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2008000400006

Bacterial community analysis of the water surface layer from a rice-planted and an unplanted flooded field
Provedor de dados: Brazilian Journal of Microbiology
Autores: Scavino,Ana Fernández; Menes,Javier; Ferrando,Lucía; Tarlera,Silvana.
The bacterial communities in floodwater, from a rice-planted and an unplanted field were characterized at the beginning (flooding stage) and at the end (harvest stage) of the rice cropping cycle. Most probable number estimations and plate counts of aerobic and anaerobic heterotrophic bacteria and of several metabolic bacterial groups (methanogens, sulfate-reducers, anaerobic sulfur and nonsulfur phototrophs, denitrifiers and ammonifiers) were similar in rice and unplanted floodwater at both sampling times. The analysis of denitrifiers and methanogens by fluorescent in situ hybridization revealed a shift in the phylogenetic affiliation only of the former group in the rice-planted floodwater. Terminal restriction fragment length polymorphism of 16S rRNA gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Floodwater; Rice; Microbial community; T-RFLP.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200023