Consulta: subjectFacets:"TLR-4"
Registros recuperados: 3
Data/hora: 11/06/2024 08:21:36

Regulation of Innate Immune Function in Bovine Oviduct Epithelial Cells in Culture: The Homeostatic Role of Epithelial Cells in Balancing Th1/Th2 Response
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: KOWSAR, Rasoul; HAMBRUCH, Nina; LIU, Jinghui; SHIMIZU, Takashi; PFARRER, Christiane; MIYAMOTO, Akio; 清水, 隆; 宮本, 明夫.
This study aimed to investigate the role of epithelial cells in regulating innate immunity in bovine oviduct epithelial cell (BOEC) culture. We studied the effect of Escherichia coli lipopolysaccharide (LPS) and its interaction with ovarian steroids, estradiol (E2) and progesterone (P4), and luteinizing hormone (LH) at concentrations observed during the preovulatory period on immune responses in BOEC culture. Immunohistochemistry of oviduct tissue showed intensive expression of Toll-like receptor-4 (TLR-4) and TLR-2 in epithelial cells. A dose of 10 ng/ml LPS stimulated TLR-4, cyclooxygenase-2 (COX-2), nuclear factor kappa B inhibitor A (NFKBIA), interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) expression, indicating an early pro-inflammatory...
Palavras-chave: Bovine oviduct epithelial cell; Ovarian steroids; TLR-4; Th1/Th2 response.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3937

Protective effect and mechanism of Lactobacillus on cerebral ischemia reperfusion injury in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Wanchao,Shi; Chen,Ma; Zhiguo,Su; Futang,Xie; Mengmeng,Shi.
The present study was designed to investigate the protective effects and mechanism of inactivated lactobacillus (ILA) on cerebral ischemia reperfusion injury (CIRI) in rats. In this experiment, 30 male Sprague Dawley rats were randomly divided into control group, IRI groups, and ILA group. A middle cerebral artery occlusion and reperfusion model was prepared. The rats were killed after 24 hours of recovery of blood flow of cerebral ischemia resulting from 60-min occlusion. The cerebral infarction volume and neurological scores were assayed by staining and behavioral observation. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were assayed by biochemical kits. Cell apoptosis was assayed by Tunnel and the Toll-like receptor (TLR)-4, IkB, and A20...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebral ischemia reperfusion injury; Inactivated Lactobacillus; Apoptosis; NF-kappa B; TLR-4.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700607

Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins
Provedor de dados: Brazilian Journal of Microbiology
Autores: Mesquita,Adriano Queiroz de; Rezende,Cintia Silva Minafra e; Mesquita,Albenones José de; Jardim,Eurione Antonio Garcia da Veiga; Kipnis,Ana Paula Junqueira.
The identification of dairy cows with greater or lower potential to develop mastits has been pursued for many years among different segments of the milk industry, including governmental organizations. Genomic studies have suggested that Single Nucleotide Polymorphisms (SNPs) within the pattern recognition receptors (PRR) could lead to different responses to pathogens, and consequently result in mastitis resistance or susceptibility. To investigate whether toll like receptor 4 (TLR4) gene is associated with subclinical mastitis in Holstein cows from a property in the state of Goiás, Brazil, TaqMan allelic discrimination and somatic cell count were performed. One hundred and fifty milk samples were analyzed for SCC and centesimal composition. Twenty percent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TLR-4; Single nucleotide polymorphism; Mastitis; Milk quality.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200034