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TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Dalla Torre,C.A.; Maciel,R.M.B.; Pinheiro,N.A.; Andrade,J.A.D.; de Toledo,S.R.C.; Villa,L.L.; Cerutti,J.M.
Measurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is safe and requires minimal equipment. In addition, we determined the sensitivity of the silver-staining method by using extracts of telomerase-positive thyroid carcinoma cell lines which were serially diluted from 5,000 to 10 cells. Telomerase activity was also assayed in 19 thyroid tumors, 2 normal controls and 27 bone marrow aspirates. The results indicate that the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Telomeric repeat amplification protocol; TRAP assay; Human thyroid cancer; Leukemia; Telomerase; Silver staining.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100009