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Molecular investigation of tRNA genes integrity and its relation to pathogenicity islands in Shiga toxin-producing Escherichia coli (STEC) strains
Provedor de dados: Genetics and Molecular Biology
Autores: Novais,Rogério Carlos; Chaves,Marcela Cassin; Gonzalez,Alice Gonçalves Martins; Andrade,João Ramos Costa.
tRNA genes are known target sites for the integration of pathogenicity islands (PAI) and other genetic elements, such as bacteriophages, into bacterial genome. In most STEC (Shiga toxin-producing Escherichia coli), the PAI called LEE (locus of enterocyte effacement) is related to bacterial virulence and is mostly associated to the tRNA genes selC and pheU. In this work, we first investigated the relationship of LEE with tRNA genes selC and pheU in 43 STEC strains. We found that 28 strains (65%) had a disrupted selC and/or pheU. Three of these strains (637/1, 650/5 and 654/3) were chosen to be submitted to a RAPD-PCR technique modified by the introduction of specific primers (corresponding to the 5'end of genes selC and pheU) into the reaction, which we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogenicity islands; TRNA; STEC; RAPD-PCR; E.coli.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400020

Partial characterization of ribosomal operons of Lactobacillus delbrueckii UFV H2b20
Provedor de dados: Brazilian Journal of Microbiology
Autores: Magalhães,Juliana Teixeira de; Floresta,Fernanda; Moraes,Célia Alencar de.
Ribosomal operons are great tools for microbe community characterization and for microorganisms relationship study, particularly in the case of the acid lactic bacteria. The ribosomal operon of the probiotic strain Lactobacillus delbrueckii UFV H2b20 was partially characterized. A genomic library of this strain was constructed and the clones with partial ribosomal operon were sub-cloned using the shot-gun method for subsequent sequencing with the forward primer. The sequence analysis revealed that the 3' end of the rDNA 16S was following by the short spacer region 1 (16S-23S) and that the 3' end of the rDNA 23S was following by the short spacer region 2 (23S-5S), which preceded the rDNA 5S. In the flanking region of the rDNA 5S gene of this operon rrn, a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ribosomal operons; Lactobacillus; TRNA; Acid lactic bacteria.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200014