Consulta: subjectFacets:"TUNEL"
Registros recuperados: 6
Data/hora: 09/06/2024 12:46:08

Prenatal Water Deprivation Induces Apoptosis in Sexual Dimorphic Nucleus of the Brain of Male New Born Sprague-Dawley Rats
Provedor de dados: 77
Autores: Chehreie,Shima; Sadri,Soheil; Khazaei,Mozafar; Ghanbari,Ali; Ayubian,Mahmood; Amiri,Sara.
Considering the size of some nuclei and area, sex hormones control the sexual development of the brain. The sexual development of the brain can also be influenced by environmental stress. This study aimed to clear the effect of prenatal water deprivation on the development of sexual dimorphic nucleus (SDN) of the brain. In this research, pregnant rats were divided into two groups (control and treated). For the treated animals, water was removed from the ewes for 48 h at the end of third trimester of gestation (19-21 days). TUNEL staining was used for detection of apoptosis in paraffin embedded diencephalon selected sections. The ratio of apoptotic cells to non- apoptotic ones was calculated as apoptotic index. Differences of apoptotic index and serum...
Tipo: Journal article
Palavras-chave: Apoptosis; Rat; SDN nucleus; Stress; TUNEL.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200032

Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against Cisplatin­Induced Apoptosis on Testis in Mice
Provedor de dados: 77
Autores: Keshtmand,Zahra; Ghanbari,Ali; Khazaei,Mozafar; Rabzia,Arezou.
Cisplatin is an anti-cancer drug used in chemotherapy. One of the limiting side effects of cisplatin is decreasing genital gland function, azoospermia and oligospermia. Tribulus terrestris (TT) has been used as an aphrodisiac. The present study amid to investigate protective effect of TT hydroalcoholic extract against cisplatin-induced apoptosis on testis in mice. Male adult mice (n=30) were divided into control and 4 experimental groups (n=6). Control group received saline, first experimental group received cisplatin (5.5 mg/kg) and other three experimental group received cisplatin (5.5 mg/kg) and different doses of hydroalcoholic extact of TT (100, 300 and 500 mg/kg/i.p) respectively. Day after the last injection, histopathology and histomorphic analysis...
Tipo: Journal article
Palavras-chave: Cisplatin; Tribulus terrestris; Apoptosis; TUNEL.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100044

Environmental quality of an estuary in Amazon delta using immunohistochemical and morphological analyses of gill as biomarkers
Provedor de dados: 3
Autores: Montes, Caroline da Silva; Ferreira, Maria Auxiliadora Pantoja; Santos, Simone do Socorro Damasceno dos; Rocha, Rossineide Martins.
In this study, histological, ultrastructural, and immunohistochemical observations of the gill tissue of two fish species (Plagioscion squamosissimus and Lithodoras dorsalis) were used as biomarkers to assess the ecological conditions of an estuary in the Amazon delta, by relating the health status of the fishes to environmental quality. The gill tissue was analyzed both qualitatively and semi-quantitatively. Marked differences in the type and severity of lesions were verified between species and among study sites. A variety of severe damages were confirmed histologically in specimens from the two impacted areas (B and C), including aneurysms, epithelial lifting, and intense hyperplasia. Electron microscopy and immunohistochemical analyses also revealed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.06.03.00-2; 2.10.07.00-4; 2.11.01.00-0 histology; TUNEL; Teleost; Barcarena; Industrial effluents ecotoxicologia.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/24541

Apoptosis of sensory neurons and satellite cells after sciatic nerve transection in C57BL/6J mice
Provedor de dados: 56
Autores: Oliveira,A.L.R.
The rate of axonal regeneration, after sciatic nerve lesion in adult C57BL/6J mice, is reduced when compared to other isogenic strains. It was observed that such low regeneration was not due just to a delay, since neuronal death was observed. Two general mechanisms of cell death, apoptosis and necrosis, may be involved. By using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) technique, we demonstrated that a large number of sensory neurons, as well as satellite cells found in the dorsal root ganglia, were intensely labeled, thus indicating that apoptotic mechanisms were involved in the death process. Although almost no labeled neurons or satellite cells were observed one week after transection, a more than ten-fold increase in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: TUNEL; Apoptosis; Axotomy; Neuronal death; Satellite cells; Isogenic mice.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300012

Rhein induces apoptosis of human gastric cancer SGC-7901 cells via an intrinsic mitochondrial pathway
Provedor de dados: 56
Autores: Li,Yiwen; Xu,Yuqing; Lei,Bo; Wang,Wenxiu; Ge,Xin; Li,Jingrui.
Rhein is a primary anthraquinone found in the roots of a traditional Chinese herb, rhubarb, and has been shown to have some anticancer effects. The aim of the present study was to investigate the effect of rhein on the apoptosis of the human gastric cancer line SGC-7901 and to identify the mechanism involved. SGC-7901 cells were cultured and treated with rhein (0, 50, 100, 150, and 200 µM) for 24, 48, or 72 h. Relative cell viability assessed by the MTT assay after treatment was 100, 99, 85, 79, 63% for 24 h; 100, 98, 80, 51, 37% for 48 h, and 100, 97, 60, 36, 15% for 72 h, respectively. Cell apoptosis was detected with TUNEL staining and quantified with flow cytometry using annexin FITC-PI staining at 48 h after 100, 200 and 300 µm rhein. The percentage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhein; SGC-7901 cells; MTT; TUNEL; Flow cytometry; Apoptosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100009

Apoptose na infecção experimental de cães domésticos com Ehrlichia canis
Provedor de dados: 65
Autores: Sanchez,Irma Ximena Barbosa; Socarras,Teresa de Jesus Oviedo; Silva,Miguel Ladino; Pereira,Nubia Braga; Machado,Rosangela Zacarias; Vasconcelos,Anilton Cesar.
A Erliquiose canina é uma zoonose causada pela Ehrlichia canis, bactéria Gram negativa de distribuição mundial. Alguns cães com erliquiose se tornam portadores assintomáticos enquanto outros desenvolvem uma doença aguda com morte rápida. A apoptose pode ser importante na eliminação de patógenos intracelulares, podendo, nas infecções por Ehrlichia sp. e Anaplasma sp., ocorrer modulação da apoptose celular para prolongar a sobrevivência desses organismos. Para avaliação do papel da apoptose na erliquiose canina, sete cães foram distribuídos em dois grupos. No Grupo inoculado, realizou-se a infecção por via intravenosa com sangue infectado com E. canis (isolado Jaboticabal), sendo realizada a inoculação com PBS estéril nos animais pertencentes ao Grupo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptose; Ehrlichia canis; N-PCR; RIFI; TUNEL; Bcl-2; Bax.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000800021