Consulta: subjectFacets:"Thermotogales"
Registros recuperados: 8
Data/hora: 17/06/2024 20:56:03

Marinitoga hydrogenitolerans sp nov., a novel member of the order Thermotogales isolated from a black smoker chimney on the Mid-Atlantic Ridge
Provedor de dados: 5
Autores: Postec, Anne; Le Breton, Claire; Fardeau, Marie-laure; Lesongeur, Francoise; Pignet, Patricia; Querellou, Joel; Ollivier, Bernard; Godfroy, Anne.
A novel, thermophilic, anaerobic bacterium that is able to tolerate hydrogen was isolated from a deep-sea hydrothermal chimney collected at the Rainbow field on the Mid-Atlantic Ridge. Cells were rod-shaped and surrounded by a sheath-like outer structure (toga); they were weakly motile by means of a polar flagellum. They appeared singly, in pairs or in short chains. They grew at 35-65 degrees C (optimum 60 degrees C), pH 4.5-8.5 (optimum pH 6.0) and 10-65 g sea salts l(-1) (optimum 30-40 g l(-1)). The isolate was organotrophic, and able to grow on various carbohydrates or complex proteinaceous substrates. Growth was not inhibited under 100% hydrogen or in the presence of 2% oxygen in the gas phase. The isolate reduces sulfur, although sulfur reduction is...
Tipo: Text
Palavras-chave: Anaerobic bacterium; Marinitoga hydrogenitolerans; Thermotogales; Thermophile; Deep sea vent.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-363.pdf

Complete genome sequence and whole-genome phylogeny of Kosmotoga pacifica type strain SLHLJ1T from an East Pacific hydrothermal sediment
Provedor de dados: 5
Autores: Jiang, Lijing; L'Haridon, Stephane; Jebbar, Mohamed; Xu, Hongxiu; Alain, Karine; Shao, Zongze.
Kosmotoga pacifica strain SLHLJ1T is a thermophilic chemoorganoheterotrophic bacterium isolated from a deep-sea hydrothermal sediment. It belongs to the physiologically homogeneous Thermotogaceae family. Here, we describe the phenotypic features of K. pacifica together with its genome sequence and annotation. The chromosome has 2,169,170 bp, organized in one contig. A total of 1897 candidate protein-encoding genes and 177 RNA genes were identified. The 16S rRNA gene sequence of this strain is distantly related to sequences of some relatives classified in the same genus (K. olearia 7.02% and K. shengliensis 7.83%), with dissimilarity percentages close to the threshold generally described for genus delineation. Nevertheless, the percentage of conserved...
Tipo: Text
Palavras-chave: Marine; Hydrothermal vent; Thermotogales; Chemoorganoheterotroph; Thermophile.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00371/48191/48324.pdf

Découverte et caractérisation des premiers virus de Thermotogales (bactéries thermophiles et anaérobies) issus de sources hydrothermales océaniques profondes
Provedor de dados: 5
Autores: Loussouarn, Julien.
Our knowledge of the viral diversity associated to procaryotic microorganisms inhabiting the deep sea hydrothermal vents is still limited. Only few studies have focused on viral abundance and impact on microbial mortality within these ecosystems. A limited number of viruses from these environments were isolated and characterized. Two viruses, PAV1 and TPV1, associated to hyperthermophilic anaerobic Archaea, Thermococcales order, have ever been described in our laboratory. The topic of this phD thesis was to extend our investigation to other deep sea vent microorganisms in order to deepen our knowledge on the marine hydrothermal virosphere. We decided to focus more precisely on the bacterial order of Thermotogales. This order is composed of anaerobic...
Tipo: Text
Palavras-chave: Sources hydrothermales océaniques profondes; Siphovirus; Plasmides; Piratage moléculaire; Plasmids; Molecular piracy; Deep sea hydrothermal vents; Thermotogales; Siphoviruses.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00609/72137/70889.pdf

Marinitoga camini gen, nov., sp nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent
Provedor de dados: 5
Autores: Wery, Nathalie; Lesongeur, Francoise; Pignet, Patricia; Derennes, Veronique; Cambon-bonavita, Marie-anne; Godfroy, Anne; Barbier, Georges.
A thermophilic, anaerobic, chemo-organotrophic sulfur-reducing bacterium, designated MV1075(T), was isolated from a deep-sea hydrothermal chimney sample collected on the Mid-Atlantic Ridge. Cells were rod-shaped with a sheath-like outer structure, motile with polar flagella and stained Gramnegative. They appeared singly, in pairs or in short chains. The temperature range for growth was 25-65 degreesC, with an optimum at 55 degreesC. Growth was observed from ph 5 to ph 9, and the optimum ph was around 7. The salinity range for growth was 15-70 g sea salt I-1 (corresponding to 10-45 g NaCl I-1), with an optimum at 30 g I-1 (20 g NaCl I-1). The isolate was able to grow on a broad spectrum of carbohydrates or complex proteinaceous substrates. Sulfur was not...
Tipo: Text
Palavras-chave: Marinitoga camini; Thermotogales; Thermophile; Deep sea hydrothermal vent.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-587.pdf

Kosmotoga pacifica sp. nov., a thermophilic chemoorganoheterotrophic bacterium isolated from an East Pacific hydrothermal sediment
Provedor de dados: 5
Autores: L'Haridon, Stephane; Jiang, Lijing; Alain, Karine; Chalopin, Morgane; Rouxel, Ouafae; Beauverger, Mikael; Xu, Hongxiu; Shao, Zongze; Jebbar, Mohamed.
A novel strictly anaerobic thermophilic heterotrophic bacterium, strain SLHLJ1T, was isolated from a Pacific hydrothermal sediment. Cells were Gram-negative coccobacilli (approximately 1.0 × 0.6 μm) with a toga. It grew at temperatures between 33 and 78 °C (optimum 70 °C). Elemental sulphur and l-cystine stimulated its growth. It contained C16:0, C16:1 ω11c, C18:0 and C18:1 ω9c as major fatty acids (&gt;5 %), 3 phospholipids and 2 glycolipids as polar lipids. Its DNA G+C content was 43.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain SLHLJ1T within the family Thermotogaceae. The novel isolate was most closely related to Kosmotoga arenicorallina (97.93 % 16S rRNA gene sequence similarity), K. olearia (92.43 %) and K. shengliensis...
Tipo: Text
Palavras-chave: Thermotogales; Kosmotoga; Kosmotoga pacifica; Hydrothermal sediment.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00171/28204/26509.pdf

Thermosipho atlanticus sp nov., a novel member of the Thermotogales isolated from a Mid-Atlantic Ridge hydrothermal vent
Provedor de dados: 5
Autores: Urios, Laurent; Cueff-gauchard, Valerie; Pignet, Patricia; Postec, Anne; Fardeau, Marie-laure; Ollivier, Bernard; Barbier, Georges.
A novel anaerobic, thermophilic and heterotrophic bacterium, designated strain DV114O(T), was isolated from a deep-sea hydrothermal vent sample from the Mid-Atlantic Ridge. The cells were non-motile straight rods, 1-8 mum long and 0-4 mum wide, surrounded by an outer sheath-like structure (toga). They grew at 45-80 degreesC (optimum 65 degreesC), pH 5-0-9-0 (optimum pH 6-0) and at sea salt concentrations of 20-60 gl(-1) (optimum 30 gl(-1)). Strain DV1140(T) was able to ferment yeast extract, peptone, brain heart infusion, gelatin, starch, galactose, arabinose, glucose, trehalose and cellobiose. The fermentation products identified on glucose in the presence of yeast extract and peptone were acetate, isovalerate and hydrogen. The G + C content of the...
Tipo: Text
Palavras-chave: Marine microorganisms; Thermosipho atlanticus; Thermotogales; Thermophile; Deep sea vent.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-362.pdf

De nouveaux systèmes hôtes-virus associés aux sources hydrothermales océaniques profondes
Provedor de dados: 5
Autores: Boyer, Coraline.
Our knowledge of the viral diversity associated to microorganisms inhabiting the deep-sea hydrothermal vents is still limited. Only a few studies have focused on viral abundance and impact on microbial mortality within these ecosystems. A limited number of viruses (6 bacterioviruses and 2 archaeoviruses) were isolated from these environments and characterized. Two viruses associated to hyperthermophilic anaerobic Archaea, from the Thermococcales order, have been described in our laboratory. In order to deepen our knowledge on the viral diversity of these extreme environments, we have extended our investigation to the bacterial order of Thermotogales. This order is composed of anaerobic chemoorganotrophic bacteria that are, for the most part,...
Tipo: Text
Palavras-chave: Bactériovirus; Sources hydrothermales profondes; Éléments génétiques mobiles; Océans profonds; Bacterioviruses; Thermotogales; Deep sea; Hydrothermal vents; Mobile genetic elements.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00609/72129/70881.pdf

Continuous enrichment culture and molecular monitoring to investigate the microbial diversity of thermophiles inhabiting deep-sea hydrothermal ecosystems
Provedor de dados: 5
Autores: Postec, Anne; Urios, Laurent; Lesongeur, Francoise; Ollivier, Bernard; Querellou, Joel; Godfroy, Anne.
The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90 degrees C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA. Additional sequences phylogenetically related to Thermococus and epsilon-Proteobacteria were detected by cloning and sequencing of 16S rDNA from two samples of the enrichment culture. In comparison, the sequences retrieved from cloning analysis from an enrichment culture...
Tipo: Text
Palavras-chave: Sequence alignment; Anaerobic digester; Thermotogales; Thermophile; Deep sea vent.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-364.pdf