Consulta: subjectFacets:"Thin-layer chromatography assay"
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Two fast screening methods (GC-MS and TLC-ChEI assay) for rapid evaluation of potential anticholinesterasic indole alkaloids in complex mixtures
Provedor de dados: 42
Autores: Vieira,Ivo J.C.; Medeiros,Walter L.B.; Monnerat,Cecilia S.; Souza,Jucimar J.; Mathias,Leda; Braz-Filho,Raimundo; Pinto,Angelo C.; Sousa,Priscila M.; Rezende,Claudia M.; Epifanio,Rosângela De A.
The pharmacotherapyfor Alzheimer's disease (AD) includes the use of acetylcholinesterase inhibitors (AChEI). Recent investigations for novel AD therapeutic agents from plants suggested that Tabernaemontana genus is a promising source of novel anticholinesterasic indole alkaloids. In this work two fast screening techniques were combined in order to easily identify novel cholinesterase inhibitors (ChEI). Gas chromatography-mass spectrometry (GC-MS) of the less polar alkaloidic fractions obtained from the acid-base extraction of the stalk of T. laeta revealed thirteen monoindole alkaloids, four of them confirmed by co-injection with previously isolated alkaloids. The others were tentatively identified by mass fragmentation analysis. By gas chromatography with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tabernaemontana; Indole alkaloids; Cholinesterase inhibitor; Thin-layer chromatography assay; Ellman´s method; GC-MS.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000300003