Consulta: subjectFacets:"Thrombin-like enzyme"
Registros recuperados: 4
Data/hora: 09/06/2024 02:19:14

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review
Provedor de dados: 80
Autores: Buchaim,Daniela Vieira; Cassaro,Claudia Vilalva; Shindo,João Vitor Tadashi Cosin; Coletta,Bruna Botteon Della; Pomini,Karina Torres; Rosso,Marcelie Priscila de Oliveira; Campos,Leila Maria Guissoni; Ferreira Jr,Rui Seabra; Barraviera,Benedito; Buchaim,Rogério Leone.
Abstract Fibrin biopolymers, previously referred as “fibrin glue” or “fibrin sealants”, are natural biomaterials with diverse applications on health. They have hemostatic, adhesive, sealant, scaffold and drug delivery properties and have become widely used in medical and dental procedures. Historically, these biomaterials are produced from human fibrinogen and human or animal thrombin, and the possibility of transmission of infectious diseases by human blood is not ruled out. In the 1990s, to overcome this problem, a new heterologous biomaterial composed of a thrombin-like enzyme purified from Crotalus durissus terrificus venom and a cryoprecipitate rich in fibrinogen extracted from buffaloes Bubalus bubalis blood has been proposed. Therefore, a systematic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrin sealant; Snake venom; Thrombin-like enzyme; Cryoprecipitate coagulum.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100207

Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview
Provedor de dados: 80
Autores: Ferreira Jr,Rui Seabra; Barros,Luciana Curtolo de; Abbade,Luciana Patrícia Fernandes; Barraviera,Silvia Regina Catharino Sartori; Silvares,Maria Regina Cavariani; Pontes,Leticia Gomes de; Santos,Lucilene Delazari dos; Barraviera,Benedito.
Abstract Hemostatic and adhesive agents date back to World War II, when homologous fibrin sealant came onto scene. Considering that infectious diseases can be transmitted via human blood, a new heterologous fibrin sealant was standardized in the 1990s. Its components were a serine protease (a thrombin-like enzyme) extracted from the venom of Crotalus durissus terrificus snakes and a fibrinogen-rich cryoprecipitate extracted from the blood of Bubalus bubalis buffaloes. This new bioproduct has been used as a coagulant, sealant, adhesive and recently as a candidate scaffold for mesenchymal stem cells and bone and cartilage repair. This review discusses the composition of a new heterologous fibrin sealant, and cites published articles related to its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrin sealant; Snake venom; Cryoprecipitate coagulum; Thrombin-like enzyme; Buffaloes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100204

Cross-neutralization of the coagulant activity of Crotalus durissus terrificus venom from the northeast of Argentina by bivalent bothropic antivenom
Provedor de dados: 80
Autores: Rodríguez,JP; Gay,CC; Fusco,LS; Gauna,MC; Acosta,OC; Leiva,LC.
Cross-neutralization of Crotalus durissus terrificus venom coagulant activity was tested using bivalent horse antivenom against Bothrops alternatus and Bothrops diporus venoms. Our in vitro and in vivo experiments showed that bothropic antivenom neutralizes the thrombin-like activity of crotalic snake venom and this cross-reaction was demonstrated by immunoassays either with whole venom or a purified thrombin-like enzyme. These results suggest common antigenic properties and, consequently, similar molecular structure among venom thrombin-like enzymes. Besides, they provide information that could be further used in the development of new antivenom formulations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thrombin-like enzyme; Snake venom; Antivenoms; Immunological cross-reaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100015

Serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom components in a rabbit
Provedor de dados: 80
Autores: Tan,NH; Ponnudurai,G; Fung,SY.
The serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom - specifically two of its components, the major hemorrhagin (rhodostoxin) and a thrombin-like enzyme - was examined in a rabbit by double-sandwich enzyme-linked immunosorbent assay (ELISA). The animal received intramuscularly a 1.0-mg/kg dose of C. rhodostoma venom. The venom level in serum peaked 12 hours after the injection, followed by a gradual decline and finally reached low rates 72 hours after administration. The serum kinetic profile of venom components, however, did not correspond to the profile of the whole C. rhodostoma venom. The serum levels of the C. rhodostoma thrombin-like enzyme increased slowly and peaked only 48 hours post-injection. Then both thrombin-like enzyme...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calloselasma rhodostoma venom; Thrombin-like enzyme; Hemorrhagin; Serum kinetics; Rabbit; ELISA.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200014