Consulta: subjectFacets:"Validation"
Registros recuperados: 57
Data/hora: 11/06/2024 03:45:07

Riesgo por inundación asociada a eventos de precipitación extraordinaria en la subcuenca del río La Antigua, Veracruz.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Larios Tlali, Hugo.
El objetivo del presente estudio fue aplicar el modelo de simulación hidráulica HEC-RAS para delimitar las zonas con riesgo a inundación en la subcuenca del río La Antigua, Ver., utilizando la metodología del Centro Nacional de Prevención de Desastres (CENAPRED) que considera la vulnerabilidad y el peligro de un sistema ante la ocurrencia de un fenómeno extremo perturbador. El modelo HEC-RAS usa ecuaciones diferenciales determinísticas que simulan el comportamiento y la dinámica de los niveles de agua en secciones transversales del cauce; su aplicación bajo condiciones de flujo no permanente y régimen mixto, permitió definir las áreas de inundación alcanzadas durante una avenida extraordinaria. Para la simulación hidráulica se construyeron hidrogramas...
Palavras-chave: Simulación hidráulica; Inundación; Hidrograma; Fenómenos hidrometeorológicos extremos; Calibración; Validación; Riesgo por inundación; Hydraulic simulation; Flood hydrograph; Extreme weather phenomena; Calibration; Validation; Flood risk; Hidrociencias; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2375

Compuestos volátiles que median la interacción entre Anthonomus eugenii Cano y Capsicum annuum L.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Velázquez González, Julio César.
Dado que el picudo del chile es el principal problema fitosanitario en el cultivo de Capsicum spp., y las medidas de control no han logrado mitigar de forma eficiente las poblaciones de este insecto, se estudiaron los siguientes objetivos con el fin de conocer las interacciones planta insecto. Primero se valido un método de recolecta de volátiles, el cual demostró ser eficiente bajo las diferentes variables analíticas estudiadas, con % de recobro de 90-100 y DER menores a 10%. En segundo lugar se evaluó el potencial atrayente de estructuras de la planta como flores, yemas, frutos en desarrollo y frutos comerciales, encontrando que machos prefieren en primer lugar flores y después yemas, y las hembras en su primera opción eligieron yemas  y después flores....
Palavras-chave: Validación; Feromona; Capsicum; Sensilla; Cromatografía; Validation; Pheromone; Chromatography; Entomología y Acarología; Doctorado.
Ano: 2011
URL: http://hdl.handle.net/10521/557

Compuestos volátiles que median la interacción entre Anthonomus eugenii Cano y Capsicum annuum L.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Velázquez González, Julio César.
Dado que el picudo del chile es el principal problema fitosanitario en el cultivo de Capsicum spp., y las medidas de control no han logrado mitigar de forma eficiente las poblaciones de este insecto, se estudiaron los siguientes objetivos con el fin de conocer las interacciones planta insecto. Primero se valido un método de recolecta de volátiles, el cual demostró ser eficiente bajo las diferentes variables analíticas estudiadas, con % de recobro de 90-100 y DER menores a 10%. En segundo lugar se evaluó el potencial atrayente de estructuras de la planta como flores, yemas, frutos en desarrollo y frutos comerciales, encontrando que machos prefieren en primer lugar flores y después yemas, y las hembras en su primera opción eligieron yemas  y después flores....
Palavras-chave: Validación; Feromona; Capsicum; Sensilla; Cromatografía; Validation; Pheromone; Chromatography; Entomología y Acarología; Doctorado.
Ano: 2011
URL: http://hdl.handle.net/10521/557

Estandarización y validación de la técnica de PCR para el diagnóstico de Salmonella typhimurium en carne de cerdo, res y pollo.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Rosas Espejel, Monzerrat.
El estudio tuvo como objetivo estandarizar y validar la técnica de Reacción en Cadena de la Polimerasa (PCR) para la detección de Salmonella typhimurium (St) en carne de cerdo, res y pollo, optimizando el tiempo y la sensibilidad con relación al método microbiológico; además se realizó el diagnóstico en estos tipos de carnes para evaluar su calidad microbiológica en la Ciudad de Texcoco. Un total de 60 muestras de carne (20 de cerdo, 20 de res y 20 de pollo) de 250 g fueron recolectadas en puntos de venta de la Ciudad de Texcoco. Las muestras recolectadas procedían de carnicerías, tianguis y supermercados. Se evaluó la sensibilidad y especificidad de la PCR para detectar St; asimismo, se validó esta técnica en relación al método microbiológico con base al...
Palavras-chave: Salmonella typhimurium; PCR; Carne; Validación; Meat; Validation; Ganadería; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2388

Is Validation of Indigenous Ecological Knowledge a Disrespectful Process? A Case Study of Traditional Fishing Poisons and Invasive Fish Management from the Wet Tropics, Australia
Provedor de dados: Ecology and Society
Autores: Gratani, Monica; School of Earth and Environmental Sciences, James Cook University; monica.gratani@jcu.edu.au; Butler, James R. A. ; CSIRO Ecosystem Sciences, EcoSciences Precinct ; james.butler@csiro.au; Royee, Frank; Malanbarra Yidinji Elder;; Valentine, Peter; School of Earth and Environmental Sciences, James Cook University;; Burrows, Damien; Australian Centre for Tropical Freshwater Research, James Cook University;; Canendo, Warren I.; CSIRO Sustainable Ecosystems and Sustainable Agriculture Flagship, ATFI;; Anderson, Alex S; Centre for Tropical Biodiversity and Climate Change, School of Marine and Tropical Biology, James Cook University; alexander.anderson@my.jcu.edu.au.
Tipo: Peer-Reviewed Reports
Palavras-chave: Comanagement; Fishing poisons; Indigenous ecological knowledge; Invasive fish; Knowledge socialization; Livelihoods; Poisonous plants; Social-ecological systems: tilapia; Traditional ecological knowledge; Validation.
Ano: 2011

Assessment of molecular recognition element for the quantification of human epidermal growth factor using surface plasmon resonance
Provedor de dados: Electronic Journal of Biotechnology
Autores: Rosti,Ira Amira; Ramanan,Ramakrishnan Nagasundara; Ling,Tau Chuan; Ariff,Arbakariya B.
Background: A method for the selection of suitable molecular recognition element (MRE) for the quantification of human epidermal growth factor (hEGF) using surface plasmon resonance (SPR) is presented. Two types of hEGF antibody, monoclonal and polyclonal, were immobilized on the surface of chip and validated for its characteristics and performance in the quantification of hEGF. Validation of this analytical procedure was to demonstrate the stability and suitability of antibody for the quantification of target protein. Results: Specificity, accuracy and precision for all samples were within acceptable limit for both antibodies. The affinity and kinetic constant of antibodies-hEGF binding were evaluated using a 1:1 Langmuir interaction model. The model...
Tipo: Journal article
Palavras-chave: Antibody; BIAcore; Biosensors; Epidermal growth factor; Validation.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600015

Hydrological simulation using SWAT model in headwater basin in Southeast Brazil
Provedor de dados: Engenharia Agrícola
Autores: Pereira,Donizete dos R.; Martinez,Mauro A.; Almeida,André Q. de; Pruski,Fernando F.; Silva,Demetrius D. da; Zonta,João H.
Hydrological models are important tools that have been used in water resource planning and management. Thus, the aim of this work was to calibrate and validate in a daily time scale, the SWAT model (Soil and Water Assessment Tool) to the watershed of the Galo creek , located in Espírito Santo State. To conduct the study we used georeferenced maps of relief, soil type and use, in addition to historical daily time series of basin climate and flow. In modeling were used time series corresponding to the periods Jan 1, 1995 to Dec 31, 2000 and Jan 1, 2001 to Dec 20, 2003 for calibration and validation, respectively. Model performance evaluation was done using the Nash-Sutcliffe coefficient (E NS) and the percentage of bias (P BIAS). SWAT evaluation was also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrological modeling; SWAT; Calibration; Validation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162014000400018

VALIDATION OF SATELLITE DERIVED LHF USING COARE_3.0 SCHEME AND TIME SERIES DATA OVER NORTH-EAST INDIAN OCEAN
Provedor de dados: Gayana
Autores: Muraleedharan,P.M.; Pankajakshan,T.; Sathe,P.V.
Time series measurement of surface meteorological parameters using an Autonomous Weather Station and Moored Buoy positioned at 10.5 N and 72 E from July to November 2000 are used to compute latent heat flux (LHF) using COARE_3.0 algorithm for validating Special Sensor Microwave Imager (SSMI) derived LHF data. Mean positive and negative biases are observed between satellite and sea truth derived LHF estimates for summer (July to Sept) and inter-monsoon (Oct-Nov.) seasons respectively. Comparison during summer season exhibits very high correlation ( 75%) and is increasing progressively with longer climatologies. Satellite underestimates higher LHF values during summer while it overestimates lower LHF values in inter-monsoon season. Root Mean Square Deviation...
Tipo: Journal article
Palavras-chave: Validation; Autonomous Weather Station; COARE_3.0 Scheme; Latent Heat Flux; Summer Monsoon.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382004000300019

AN INTERACTIVE SOFTWARE PACKAGE FOR VALIDATING SATELLITE DATA
Provedor de dados: Gayana
Autores: Muraleedharan,P.M.; Pankajakshan,T.
Earth observation satellite launched in the last three decades have made vital contributions to the understanding of the planet. The earth system consisting of land, oceans and atmosphere is linked together by a number of complicated processes. Potential of microwave sensors in studying the boundary layer dynamics of the ocean-atmosphere system is well documented. Technology has revolutionized the application of such sensors and several missions are planned to take off in near future for meeting specific objectives. Launching of such sensors need to be followed by an extensive validation campaign for providing accurate and reliable information to the users. A PC-based interactive system has been developed and presented here for validating satellite mounted...
Tipo: Journal article
Palavras-chave: Validation; Microwave sensor; Autocorrelation; Sea surface temperature; Wind speed; Columnar water vapor.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382004000300018

Sensitivity, advantages, limitations, and clinical utility of targeted next-generation sequencing panels for the diagnosis of selected lysosomal storage disorders
Provedor de dados: Genetics and Molecular Biology
Autores: Málaga,Diana Rojas; Brusius-Facchin,Ana Carolina; Siebert,Marina; Pasqualim,Gabriela; Saraiva-Pereira,Maria Luiza; Souza,Carolina F.M de; Schwartz,Ida V.D.; Matte,Ursula; Giugliani,Roberto.
Abstract Lysosomal storage disorders (LSDs) constitute a heterogeneous group of approximately 50 genetic disorders. LSDs diagnosis is challenging due to variability in phenotype penetrance, similar clinical manifestations, and a high allelic heterogeneity. A powerful tool for the diagnosis of the disease could reduce the “diagnostic odyssey” for affected families, leading to an appropriate genetic counseling and a better outcome for current therapies, since enzyme replacement therapies have been approved in Brazil for Gaucher, Fabry, and Pompe diseases, and are under development for Niemann-Pick Type B. However, application of next-generation sequencing (NGS) technology in the clinical diagnostic setting requires a previous validation phase. Here, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ion Torrent; Molecular diagnostics; Next-generation sequencing; Lysosomal storage disorders; Validation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200197

Application du modèle MARS-3D à la rade de Toulon. Comparaison à des données historiques
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Tessier, Caroline.
Développé par le laboratoire DEL/AO à Brest et appliqué à la rade de Toulon, le modèle MARS-3D a été installé au laboratoire de Toulon. L'objectif est de mettre en route son application à Toulon et de contribuer à l'analyse en cours des résultats du modèle, en les· comparant aux mesures historiques. Dans ce rapport sont présentés les travaux faits pour réaliser ce double objectif: l'apprentissage du modèle dans sa conception et son utilisation, l'analyse des résultats et la comparaison avec des mesures ainsi qu'une simulation de traceurs. La comparaison du modèle avec les données a été réalisée sur la base des enregistrements faits en 1986-1987 en un point de mesure au centre de la grande rade. Or, l'emboîtement des modèles crée une contrainte sur le...
Tipo: Text
Palavras-chave: Modèle; Tridimensionnel; Méditerranée; Hydrodynamique; Validation; Toulon.
Ano: 2001
URL: https://archimer.ifremer.fr/doc/00431/54248/59141.pdf

GF-3 SAR Ocean Wind Retrieval: The First View and Preliminary Assessment
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Wang, He; Yang, Jingsong; Mouche, Alexis; Shao, Weizeng; Zhu, Jianhua; Ren, Lin; Xie, Chunhua.
Gaofen-3 (GF-3) is the first Chinese civil C-band synthetic aperture radar (SAR) launched on 10 August 2016 by the China Academy of Space Technology (CAST), which operates in 12 imaging modes with a fine spatial resolution up to 1 m. As one of the primary users, the State Oceanic Administration (SOA) operationally processes GF-3 SAR Level-1 products into ocean surface wind vector and plans to officially release the near real-time SAR wind products in the near future. In this paper, the methodology of wind retrieval at C-band SAR is introduced and the first results of GF-3 SAR-derived winds are presented. In particular, the case of the coastal katabatic wind off the west coast of the U.S. captured by GF-3 is discussed. The preliminary accuracy assessment of...
Tipo: Text
Palavras-chave: GF-3; Synthetic aperture radar (SAR); Ocean surface wind; Validation.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00396/50731/51435.pdf

Validation of the TOPEX rain algorithm: Comparison with ground-based radar - art. no. 4038
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Mcmillan, A; Quartly, G; Srokosz, M; Tournadre, Jean.
[1] Recently developed algorithms have shown the potential recovery of rainfall information from spaceborne dual-frequency altimeters. Given the long mission achieved with TOPEX and the prospect of several other dual-frequency altimeters, we need to validate the altimetrically derived values so as to foster their integration with rain information from different sensors. Comparison with some alternative climatologies shows the bimonthly means for TOPEX to be low. Rather than apply a bulk correction we investigate individual rain events to understand the cause of TOPEX's underestimation. In this paper we compare TOPEX with near-simultaneous ground-based rain radars based at a number of locations, examining both the detection of rain and the quantitative...
Tipo: Text
Palavras-chave: Attenuation; Validation; Rain; Satellite remote sensing; TOPEX.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-695.pdf

Short Technical Report for OsHV-1 detection and quantification by Real Time Polynerase Chain Reaction using OsHV-1 DNA polymerase sequence
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pepin, Jean-francois.
The present report completes a previously described procedure routinely used for the detection and quantification of OsHV-1 by Sybr green Real Time Polymerase Chain Reaction targeting the OsHV-1 DNA polymerase sequence. More particularly, this document brings information about analytical sensitivity, quantitation limit, assay precision, reproducibility of the technique.
Tipo: Text
Palavras-chave: PCR quantitative HDP-F/R; Virus OsHV-1; Diagnostic; DNA polymerase; Validation; Real-Time PCR HDP-F/R; OsHV-1; Diagnostic; DNA polymerase; Validation.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00137/24814/22900.pdf

Modèle CROCO. Coastal and Regional Ocean Community model. Mise en oeuvre et performances pour les configurations côtières
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Heyraud, Catherine.
Les performances du modèle communautaire CROCO sont évaluées grâce à un jeu de données in-situ complet (courant, hauteur d’eau, température, salinité) et comparées à celles du modèle MARS-3D. Il apparaît que les modèles ont un comportement hydro-dynamique proche avec des divergences liées au coeur numérique de CROCO (schéma temporel, schéma d’advection). A ce stade, ces divergences n’apparaissent pas discriminantes pour CROCO.
Tipo: Text
Palavras-chave: CROCO; MARS; Hydrodynamique; Validation.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00470/58158/60657.pdf

Age estimation in common sole Solea solea larvae: validation of daily increments and evaluation of a pattern recognition technique
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lagardere, Françoise; Troadec, Herve.
Close similarities have been found between the otoliths of sea-caught and laboratory-reared larvae of the common sole Solea solea (L.), given appropriate temperatures and nourishment of the latter. But from hatching to mouth formation. and during metamorphosis, sole otoliths have proven difficult to read because the increments may be less regular and low contrast. In this study, the growth increments in otoliths of larvae reared at 12 degrees C were counted by light microscopy to test the hypothesis of daily deposition, with some results verified using scanning electron microscopy (SEM), and by image analysis in order to compare the reliability of the 2 methods in age estimation. Age was first estimated (in days posthatch) from light micrographs of whole...
Tipo: Text
Palavras-chave: Larval sole; Solea solea; Otolith; Daily increment; Validation; Image analysis.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00337/44785/44488.pdf

A Multivariate Regression Approach to Adjust AATSR Sea Surface Temperature to In Situ Measurements
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Tandeo, Pierre; Autret, Emmanuelle; Piolle, Jean-francois; Tournadre, Jean; Ailliot, Pierre.
The Advanced Along-Track Scanning Radiometer (AATSR) onboard Envisat is designed to provide very accurate measurements of sea surface temperature (SST). Using colocated in situ drifting buoys, a dynamical matchup database (MDB) is used to assess the AATSR-derived SST products more precisely. SST biases are then computed. Currently, Medspiration AATSR SST biases are discrete values and can introduce artificial discontinuities in AATSR level-2 SST fields. The new AATSR SST biases presented in this letter are continuous. They are computed, for nighttime and best proximity confidence data, by linear regression with different MDB covariables (wind speed, latitude, aerosol optical depth, etc.). As found, the difference between dual-view and nadir-only SST...
Tipo: Text
Palavras-chave: Validation; Sea surface temperature (SST); Remote sensing; Advanced Along Track Scanning Radiometer (AATSR).
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6135.pdf

Performance of viruses and bacteriophages for fecal source determination in a multi-laboratory, comparative study
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Harwood, Valerie J.; Boehm, Alexandria B.; Sassoubre, Lauren M.; Vijayavel, Kannappan; Stewart, Jill R.; Fong, Theng-theng; Caprais, Marie-paule; Converse, Reagan R.; Diston, David; Ebdon, James; Fuhrman, Jed A.; Gourmelon, Michele; Gentry-shields, Jennifer; Griffith, John F.; Kashian, Donna R.; Noble, Rachel T.; Taylor, Huw; Wicki, Melanie.
An inter-laboratory study of the accuracy of microbial source tracking (MST) methods was conducted using challenge fecal and sewage samples that were spiked into artificial freshwater and provided as unknowns (blind test samples) to the laboratories. The results of the Source Identification Protocol Project (SIPP) are presented in a series of papers that cover 41 MST methods. This contribution details the results of the virus and bacteriophage methods targeting human fecal or sewage contamination. Human viruses used as source identifiers included adenoviruses (HAdV), enteroviruses (EV), norovirus Groups I and II (NoVI and NoVII), and polyomaviruses (HPyVs). Bacteriophages were also employed, including somatic coliphages and F-specific RNA bacteriophages...
Tipo: Text
Palavras-chave: Virus; Bacteriophage; Water quality; Fecal pollution; Validation.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00171/28197/27786.pdf

Selection and validation of a complex fishery model using an uncertainty hierarchy
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lehuta, Sigrid; Petitgas, Pierre; Mahevas, Stephanie; Huret, Martin; Vermard, Youen; Uriarte, Andres; Record, Nicholas R.
Assessing the validity of a model is essential for its credibility especially when the model is used as decision making tool. Complex dynamic fishery models are recommended to investigate the functioning of fisheries and to assess the impact of management strategies, particularly spatial fishing regulations. However, their use is limited due to the difficulty and computational cost of parameterizing and gaining confidence, particularly for parameter rich models. These difficulties are compounded by uncertainty regarding parameter values, many of which are often taken from literature or estimated indirectly. Here we propose a methodology to improve confidence and understanding in the model, easily transferable to any complex model. The approach combines...
Tipo: Text
Palavras-chave: Bay of Biscay anchovy; Dynamic fishery model; Model selection; Uncertainty; Validation.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00148/25965/24150.pdf

Modélisation hydrodynamique de la dispersion à court terme dans une mer macrotidale: validation par des mesures à haute résolution de radiotraceurs solubles
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bailly Du Bois, Pascal; Dumas, Franck.
DISPRO project aims to validate hydrodynamic models dispersion parameters by field data for the short-term (hours to week) and short distances (100 meters to 30 km). This project is based on a 2D instantaneous currents model with a mesh size of 110 m, coupled with high frequency field data collected in the release plume. At this step of the works, measurements confirm the reliability of the model during six hours following a release. This validation made possible developpement of models with known incertitude to simulate dispersion of dissolved substances in realistic conditions for normal or accidental situations in macrotidal seas.
Tipo: Text
Palavras-chave: Validation; Tritium; Dispersion; North sea; Hydrodynamic model; Validation; Tritium; Dispersion; Manche; Modèle.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-564.pdf

Error Analysis on ESA's Envisat ASAR Wave Mode Significant Wave Height Retrievals Using Triple Collocation Model
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Wang, He; Zhu, Jianhua; Yang, Jingsong.
Nowadays, spaceborne Synthetic Aperture Radar (SAR) has become a powerful tool for providing significant wave height (SWH). Traditionally, validation of SAR derived SWH has been carried out against buoy measurements or model outputs, which only yield an inter-comparison, but not an "absolute" validation. In this study, the triple collocation error model has been introduced in the validation of Envisat ASAR derived SWH products. SWH retrievals from ASAR wave mode using ESA's algorithm are validated against in situ buoy data, and wave model hindcast results from WaveWatch III wave model, covering a period of six years. From the triple collocation validation analysis, the impacts of the collocation distance and water depth on the error of ASAR SWH are...
Tipo: Text
Palavras-chave: Envisat ASAR; Significant wave height; Validation; Triple collocation.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00249/36019/34545.pdf

Validation of EN ISO method 15216-Part 1-Quantification of hepatitis A virus and norovirus in food matrices
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lowther, J. A.; Bosch, A.; Butot, S.; Ollivier, Joanna; Maede, D.; Rutjes, S. A.; Hardouin, G.; Lombard, B.; In'T Veld, P.; Leclercq, A.
Hepatitis A virus (HAV) and norovirus are important agents of food-borne human viral illness, with common vehicles including bivalve molluscan shellfish, soft fruit and various vegetables. Outbreaks of viral illness due to contamination of the surfaces of foods, or food preparation surfaces by for example infected food handlers are also common. Virus analysis of food matrices can contribute towards risk management for these hazards and a two-part technical specification for determination of Hepatitis A virus and norovirus in food matrices (ISO/TS 15216:2013) was published jointly by the European Committee for Standardisation and the International Organization for Standardization in 2013. As part of the European Mandate No. M381 to validate 15 standards in...
Tipo: Text
Palavras-chave: Norovirus; Hepatitis A virus; Real-time RT-PCR; Validation; Standardization; Bivalve molluscan shellfish; Soft fruit; Vegetables; Bottled water; Surfaces.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00476/58776/61442.pdf

Toward Long-Term Aquatic Science Products from Heritage Landsat Missions
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pahlevan, Nima; Balasubramanian, Sundarabalan V.; Sarkar, Sudipta; Franz, Bryan A.
This paper aims at generating a long-term consistent record of Landsat-derived remote sensing reflectance (R-rs) products, which are central for producing downstream aquatic science products (e.g., concentrations of total suspended solids). The products are derived from Landsat-5 and Landsat-7 observations leading to Landsat-8 era to enable retrospective analyses of inland and nearshore coastal waters. In doing so, the data processing was built into the SeaWiFS Data Analysis System (SeaDAS) followed by vicariously calibrating Landsat-7 and -5 data using reference in situ measurements and near-concurrent ocean color products, respectively. The derived R rs products are then validated using (a) matchups using the Aerosol Robotic Network (AERONET) data...
Tipo: Text
Palavras-chave: Landsat; Coastal/inland waters; Atmospheric correction; Vicarious calibration; Validation; Water quality; Time-series applications.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00585/69696/67581.pdf

Assessment of SARAL/AltiKa Wave Height Measurements Relative to Buoy, Jason-2, and Cryosat-2 Data
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Hito Sepulveda, Hector; Queffeulou, Pierre; Ardhuin, Fabrice.
SARAL/AltiKa GDR-T are analyzed to assess the quality of the significant wave height (SWH) measurements. SARAL along-track SWH plots reveal cases of erroneous data, more or less isolated, not detected by the quality flags. The anomalies are often correlated with strong attenuation of the Ka-band backscatter coefficient, sensitive to clouds and rain. A quality test based on the 1Hz standard deviation is proposed to detect such anomalies. From buoy comparison, it is shown that SARAL SWH is more accurate than Jason-2, particularly at low SWH, and globally does not require any correction. Results are better with open ocean than with coastal buoys. The scatter and the number of outliers are much larger for coastal buoys. SARAL is then compared with Jason-2 and...
Tipo: Text
Palavras-chave: Radar altimeter; Significant wave height; Satellite; SARAL; AltiKa; Validation.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00286/39675/38199.pdf

Contribution à la validation de la technique de PCR pour la détection du parasite Bonamia ostreae chez l'huître plate, Ostrea edulis
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Dubois, Rodolphe.
Bonamia ostreae, l'agent de la bonamiose, est un parasite intracellulaire ayant pour cible les hémocytes (cellules du système de défense) de l'huître plate ostrea edulis. Il induit chez son hôte des lésions branchiales, telles que des perforations ou des indentations pouvant aller jusqu'à la mort du mollusque. Le travail réalisé au cours de ce stage s'inscrivait dans une démarche de validation de méthode de PCR (Polymerase Chain Reaction) pour la détection de ce parasite. Les analyses ont porté sur un lot de trois cent onze huîtres plates ostrea edulis adultes (2-3 ans), issus de captage naturel et provenant de Quiberon (Bretagne). Pour chaque individu, une extraction d'ADN a été réalisée sur un échantillon de coeur et de branchies. Afin de déterminer si...
Tipo: Text
Palavras-chave: Bonamia Ostreae; Agent pathogène; Bonamiose; Hémocytes; Huître plate; Ostrea edulis; PCR; Validation.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00369/47989/48017.pdf

Validation of in situ hybridisation and histology assays for the detection of the oyster parasite Marteilia refringens
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Thebault, Anne; Bergman, S; Pouillot, R; Le Roux, Frédérique; Berthe, Franck.
An in situ hybridisation technique has been developed for the detection of infection in oysters with Marteilia refringens with particular emphasis on light infections or confirmation of suspected cases by means of histology. Although validation of new diagnostic methods is usually achieved by comparison with standard techniques, in our case the sensitivity and specificity of the standard (histology) had not previously been established. Another point to consider is that surveillance and monitoring frequently target populations displaying different levels of prevalence under different field conditions. The objective of our study was to evaluate the sensitivity and specificity values of in situ hybridisation and histology for the detection of M refringens,...
Tipo: Text
Palavras-chave: Ostrea edulis; Marteilia refringens; Latent models; Specificity; Sensitivity; Validation; Diagnostic.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-3624.pdf

ProVal: A New Autonomous Profiling Float for High Quality Radiometric Measurements
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Leymarie, Edouard; Penkerc'H, Christophe; Vellucci, Vincenzo; Lerebourg, Christophe; Antoine, David; Boss, Emmanuel; Lewis, Marion R.; D'Ortenzio, Fabrizio; Claustre, Herve.
An efficient system to produce in situ high quality radiometric measurements is compulsory to rigorously perform the vicarious calibration of satellite sensors dedicated to Ocean Color Radiometry (OCR) and to validate their derived products. This requirement is especially needed during the early stages of an OCR satellite activity or for remote areas poorly covered by oceanographic cruises with possible bio-optical anomalies. Taking advantage of Argo's profiling float technology, we present a new autonomous profiling float dedicated to in situ radiometric measurements. The float is based on the Provor CTS5 (manufacturer NKE) with an added novel two protruding arm design allowing for sensor redundancies, shading mitigation and near-surface data. Equipped...
Tipo: Text
Palavras-chave: Autonomous profiling floats; Remote sensing reflectance; Ocean color; Validation; System vicarious calibration; Austral ocean; Mediterranean Sea; Radiometric measurement.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00585/69694/67579.pdf

An Unstructured Numerical Model to Study Wind-Driven Circulation Patterns in a Managed Coastal Mediterranean Wetland: The Vaccares Lagoon System
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boutron, Olivier; Bertrand, Olivier; Fiandrino, Annie; Hoehener, Patrick; Sandoz, Alain; Cherain, Yves; Coulet, Eric; Chauvelon, Philippe.
The spatiotemporal structure of wind-driven circulation patterns and associated water exchanges can drive important bio-hydrodynamic interactions in shallow lagoons. The Vaccares lagoon system is a complex shallow hydrosystem located in the central part of the Rhone Delta (France). It is internationally recognized as part of a biosphere reserve within the framework of UNESCO's Man and Biosphere Programme, and as a RAMSAR site. Due to its frequent occurrence in this area, and considering the shallowness of the Vaccares lagoon system, wind is assumed to play a major role in the hydrodynamic and biological processes. In this study, a hydrodynamic model was developed to investigate the structure of wind-driven circulations in the Vaccares lagoon system, to...
Tipo: Text
Palavras-chave: Coastal lagoon; Mathematical model; Hydrodynamics; Calibration; Validation; Wind-driven circulation.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00303/41382/40558.pdf

Standardization and validation of Dot-ELISA assay for Paracoccidioides brasiliensis antibody detection
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Kamikawa,Camila Mika; Mendes,Rinaldo Poncio; Vicentini,Adriana Pardini.
Abstract  Background  Paracoccidioidomycosis (PCM) is a neglected systemic mycosis caused by a dimorphic fungus of the Paracoccidioides genus. The standard diagnosis is based on isolation of the fungi in culture, and by microscopic visualization of characteristic multiple budding yeast cells in biological samples. However, in some situations, access to the site of injury prevents the collection of biological material. A variety of immuno-serological techniques has proven useful for allowing inferring diagnosis with a certain degree of certainty, thus optimizing time. The aim of this study was to standardize and validate the Dot-ELISA (DE) assay, comparing it with the serological standard, double immunodiffusion (DI).  Methods  In order to standardize the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paracoccidioidomycosis; Paracoccidioides brasiliensis; Immunodiagnostic tool; Dot-ELISA; Validation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100305

Systematic notes on Asian birds. 16. The type of Pitta baudii Müller &amp; Schlegel, 1839 (Pittidae)
Provedor de dados: Naturalis Digital Academic Repository
Autores: Dekker, R.W.R.J.; Dickinson, E.C.
Further research into the type of Pitta baudii Müller &amp; Schlegel, 1839, showed that Dickinson et al. (2000) erred in referring to the National Museum of Natural History in Leiden as the collection where a type specimen of this taxon is kept. The correction of the date of description to 1839 has revealed that the four selected specimens cannot be types. The museum received them too late. The confusion has been traced to one of the four being labelled “type”. This we find refers to the illustration by J. Wolf published in June 1845. The 1839 plate was suppressed and the original type relative to this taxon as illustrated therein has not been traced; a depiction of this is therefore of considerable importance. The suppressed plate has been located and is...
Tipo: Article / Letter to the editor
Palavras-chave: Pitta baudii; Type-specimen; Validation; Temminck; 42.83.
Ano: 2001
URL: http://www.repository.naturalis.nl/record/219915

Calibration and Validation of SWAT for the Upper Maquoketa River Watershed
Provedor de dados: Research in Agricultural & Applied Economics (AgEcon Searh)
Autores: Kanwar,   Ramesh S.; Reungsang,   Pipat; Jha,   Manoj K.; Gassman,   Philip W.; Ahmad,   Khalil; Saleh,   Ali.
A validation study has been performed using the Soil and Water Assessment Tool (SWAT) model with data collected for the Upper Maquoketa River Watershed (UMRW), which drains over 16,000 ha in northeast Iowa. This validation assessment builds on a previous study with nested modeling for the UMRW that required both the Agricultural Policy EXtender (APEX) model and SWAT. In the nested modeling approach, edge-of-field flows and pollutant load estimates were generated for manure application fields with APEX and were then subsequently routed to the watershed outlet in SWAT, along with flows and pollutant loadings estimated for the rest of the watershed routed to the watershed outlet. In the current study, the entire UMRW cropland area was simulated in SWAT, which...
Tipo: Working or Discussion Paper
Palavras-chave: Calibration; Modeling; Nitrate; SWAT; Validation; Water quality; Resource /Energy Economics and Policy.
Ano: 2005
URL: http://purl.umn.edu/18440

Using near infrared spectroscopy to predict metabolizable energy of corn for pigs
Provedor de dados: Scientia Agricola
Autores: Ferreira,Silvia Letícia; Vasconcellos,Ricardo Souza; Rossi,Robson Marcelo; Paula,Vinicius Ricardo Cambito de; Fachinello,Marcelise Regina; Huepa,Laura Marcela Díaz; Pozza,Paulo Cesar.
ABSTRACT: The chemical composition of corn is variable and the knowledge of its chemical and energetic composition is required for an accurate formulation of the diet. This study aimed to determine the chemical composition, that is, dry matter (DM), mineral matter (MM), neutral detergent fiber (NDF), acid detergent fiber (ADF), ether extract (EE), crude protein (CP), gross energy (GE) and energetic values of different varieties (batches) of corn and validate mathematical models to predict the metabolizable energy values (ME) of corn for pigs using near infrared spectroscopy (NIRS). Corn samples were scanned in the spectrum range between 1,100 and 2,500 nm, the model parameters were estimated by the modified partial least squares (MPLS) method. Ten...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemical composition; Prediction equations; Validation; Swine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000600486

Cadmium voltametric quantification in table chocolate produced in Chiquinquira-Boyaca, Colombia
Provedor de dados: Acta Agronómica
Autores: Chaparro-Acuña,Sandra Patricia; Vargas-Moreno,Paola Andrea; Silva-Gómez,Leonardo Andrés; Cárdenas,Oswaldo Eliécer.
Abstract: Bioaccumulation of heavy metals such as cadmium has been a major concern in scientific communities and international food organizations, given the great toxicological risk to the consumer, and in many places there is no detailed record of its actual content. In this way, the need arises to carry out a study and registration of the concentration of this metal in products such as table chocolate, of great consumption at regional and national level. Likewise, we seek to have effective quantification tools and a reliable and affordable method to achieve the aim of this research. In this research, Cadmium content in powdered and granulated table chocolate was determined, elaborated and commercialized in the municipality of Chiquinquira,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food; Cocoa; Electrochemistry; Heavy metals; Validation.
Ano: 2017
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000200172

Calibration and validation of the aquacrop model for the culture lettuce (lactuca sativa l.) under fertilization levels in pluvial condition
Provedor de dados: Agricultural Engineering International : The CIGR e-journal
Autores: Amirouche, mawhoub.
The search for decision-making tools is essential in order to control agricultural practices and plan for sustainable agriculture that respects the environment. To this end, FAO has developed an evaluation model called AquaCrop, which covers most technical routes, including fertilization. In this perspective, this study focuses on the AquaCrop model (v6.1) to calibrate it for the simulation of lettuce crop yields (Lactuca sativa L), under different fertilization levels for the trial periods (2015/2016) and (2016/2017). Four levels of fertilization were examined under rain conditions, namely T1 (0 kg N/ha), T2 (60 kg N/ha), T3 (120 kg N/ha) and T4 (180 kg N/ha). The model performance was evaluated using the coefficient of determination (R2), the square root...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AquaCrop; Lettuce; Calibration; Validation; Fertility stress; Water productivity.
Ano: 2021
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/6195

Development and validation of empirical models for the prediction of selected antioxidants in stored oranges
Provedor de dados: Agricultural Engineering International : The CIGR e-journal
Autores: OLUSHOLA, SUNMONU MUSLIU; ADESOJI, OLANIYAN MATHEW; JUNIOR, ADUBA JOSEPH.
Abstract: Empirical models were developed to predict some selected antioxidants present in stored oranges under a modified atmosphere.  Three sets of four different types of passive evaporative cooling structures made of two different materials; clay and aluminium were designed and constructed as part of the study.  One set consisted of four separate cooling chambers.  Two cooling chambers were made with aluminium container (cylindrical and rectangular shapes) and the other two were made of clay container (cylindrical and rectangular).  These four containers were separately inserted inside a bigger clay pot inter-spaced with clay soil of 5 cm (to form tin-in-pot, pot-in-pot, tin-in-wall and wall-in wall) with the outside structure wrapped with jute sack....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agricultural Engineering/ Food Engineering model; Validation; Predicted; Structures; Oranges.
Ano: 2014
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2757

Computational Modeling of Trombe Wall Solar Chick Brooding House for Optimal Poultry Production
Provedor de dados: Agricultural Engineering International : The CIGR e-journal
Autores: Ohagwu, Chukwuemeka Jude; Okonkwo, Ifeanyi Wilfred; Mbah, Godwin Christopher Ezike; Njoku, Howard Okezie.
Computational modeling, simulation and validation were carried out on Trombe wall solar chick brooder used for optimal poultry production. Predictive linear differential equations based on physical, mathematical principles and relationships governing thermo-physical properties of the components of the brooder were formulated, discretized and expressed in their finite different forms using SCILAB 5.5.2. T-test statistics was carried out on predicted and measured mean data: temperature of Trombe wall glaze (TG1), temperature of pebble glaze (TG2), temperature of the pebble bed bin (Tp), temperature of the brooding room (TBr), and temperature of the Trombe wall outer surface (Tw (outer surface))then separated using Levene test for equality. The model...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Computational modeling; Passive Trombe wall solar chick brooder; Simulation; Validation; Poultry production.
Ano: 2021
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/6833

Development of deficit irrigation for maize crop under drip irrigation in samaru-nigeria
Provedor de dados: Agricultural Engineering International : The CIGR e-journal
Autores: Ezekiel, Oiganji.
In the past, outcome of any water management strategy could only be known after field experiment, in recent times, means of evaluating the implications of irrigation schedules without field experiment is fast gaining grounds with the use of models.  This research work present a scenarios studies for different developed irrigation scheduling options for a drip irrigated maize crop at the Institute for Agricultural Research (IAR) irrigation farm Samaru-Nigeria during 2013 and 2014 cropping season using a computer-based model.  Aqua Crop was calibrated and validated with data obtained from the field, it was further used to generate scenario of different irrigation scheduling outcome. Grain and biomass yields, harvest index, seasonal evapotranspiration and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agricultural Engineering/ soil and water conservation engineering Aqua Crop model; Calibration; Validation; Irrigation schedulling; Water productivity.
Ano: 2017
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/3752

Validation of EGY-DRIP program Application in Determining the Optimum Lateral Length
Provedor de dados: Agricultural Engineering International : The CIGR e-journal
Autores: Pibars, Sabreen KH; Imam, Hisham M.
In drip irrigation systems the lateral lines are the pipes on which the emitters are installed. The accurate design of drip irrigation laterals needs to determine the total energy losses that include the pipe friction losses along the lateral line and minor losses due to emitter connections. A friendly user interface computer application named EGY-DRIP had developed for drip irrigation system design. The program had written in Microsoft visual basic 2013 to carry out hydraulic calculations of drip irrigation sub-unit design. The hydraulic calculation based on inputs of water properties, quality, soil characteristics, reference evapotranspiration, crop factors, and irrigation system components (drippers, pipes, and fittings). The program supported with an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Validation; Drip irrigation system; EGY-DRIP Program; Friction losses.
Ano: 2019
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/5625

Performance assessment of the database downscaled ocean waves (DOW) on Santa Catarina coast, South Brazil
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Silva,Paula G.; Klein,Antonio H.F.; González,Mauricio; Gutierrez,Omar; Espejo,Antonio.
This work presents a validation of wave parameters from the new sixty years Downscaled Ocean Waves (DOW) reanalysis database. This study compares quantiles of the Gumbel distribution of Hs (significant wave height) and Tp (peak period) from simulated data with an 11 months' time series obtained from a buoy moored seaward on the Santa Catarina coast. Analysis by means of Gumbel distribution quantiles allows more weight to be given to the highest values of the time series, which are especially important in design projects. The statistical parameters used to verify the fit between the measured and the modeled data included: RMSE, BIAS, Scatter Index and Pearson Correlation Coefficient. Mean direction (θm) validation was conducted qualitatively. The database...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DOW; Validation; Wave reanalysis; WAVEWATCHIII.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200623

Pear quality characteristics by Vis / NIR spectroscopy
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Machado,Nicácia P.; Fachinello,José C.; Galarça,Simone P.; Betemps,Débora L.; Pasa,Mateus S.; Schmitz,Juliano D.
Recently, non-destructive techniques such as the Vis / NIR spectroscopy have been used to evaluate the characteristics of maturation and quality of pears. The study aims to validate the readings by the Vis / NIR spectroscopy as a non-destructive way to assess the qualitative characteristics of pear cultivars 'Williams', 'Packams' and 'Carrick', produced according to Brazilian conditions. The experiment was conducted at the Pelotas Federal University, UFPel, in Pelotas / RS, and the instrument used to measure the fruit quality in a non-destructive way was the NIR- Case spectrophotometer (SACMI, Imola, Italy). To determine pears' soluble solids (SS) and pulp firmness (PF), it was established calibration equations for each variety studied, done from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calibration; Firmness; Pear; Soluble solids; Validation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300027

Validation of an HPLC-UV method for the identification and quantification of bioactive amines in chicken meat
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Assis,D.C.S.; Menezes,L.D.M.; Lima,A.L.; Klein,R.W.T.; Heneine,L.G.D.; Lara,L.J.C.; Teixeira,L.V.; Ornellas,C.B.D.; Cançado,S.V.; Figueiredo,T.C.
ABSTRACT A high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method was validated for the study of bioactive amines in chicken meat. A gradient elution system with an ultraviolet detector was used after extraction with trichloroacetic acid and pre-column derivatization with dansyl chloride. Putrescine, cadaverine, histamine, tyramine, spermidine, and spermine standards were used for the evaluation of the following performance parameters: selectivity, linearity, precision, recovery, limits of detection, limits of quantification and ruggedness. The results indicated excellent selectivity, separation of all amines, a coefficient of determination greater than 0.99 and recovery from 92.25 to 102.25% at the concentration of 47.2mg.kg-1,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Validation; Chicken meat; Bioactive amines; HPLC-UV.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000300805

Fourier Transform Infrared Spectroscopy (FTIR) for MUN analysis in normal and adulterated Milk
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Oliveira,M.C.P.P.; Silva,N.M.A.; Bastos,L.P.F.; Fonseca,L.M.; Cerqueira,M.M.O.P.; Leite,M.O.; Conrrado,R.S.
The objective of this study was to evaluate the CombiScope FTIR equipment based on Fourier Transform Infrared methodology (FTIR), to assess the content of milk urea nitrogen (MUN) in Brazil. Repeatability and reproducibility of CombiScopeTM FTIR (Delta Instruments), and comparison with an enzymatic automated method (Chemspec® 150; Bentley Instruments) were tested to measure raw milk urea nitrogen (MUN). Additionally, MUN levels stability after storage of raw milk samples at 4ºC, and 20ºC for up to 15 days, and capability and precision to detect extraneous urea added as an adulterant to the milk were evaluated by FTIR equipment. There was a high correlation coefficient for the analysis of MUN by FTIR equipment, when compared with the automated enzymatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Milk; Validation; Milk urea nitrogen; Infrared spectroscopy; FTIR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000500037

Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration
Provedor de dados: Biological Research
Autores: MAINARDES,RUBIANA M; PALMIRA D. GREMIÃO,MARIA.
The development and validation of a simple and accurate method based on HPLC with ultraviolet detection for the quantification of zidovudine in rat plasma and its application to a pharmacokinetic study following a single intranasal dose zidovudine is described. Zidovudine was extracted from the plasma using a single-step deproteinization. Chromatographic separation of zidovudine from interfering components was achieved with a C-18 reverse phase column, a mobile phase consisting of a mixture of sodium acetate buffer (55mM) with pH adjusted to 7.0 and acetonitrile (91:9 v/v) and UV detection set at 265 nm. The method was linear from 100 to 10000 ng.mL"¹ (r² &gt; 0.9995), and zidovudine had a mean recovery from plasma of 92.8%. The coefficient of variation of...
Tipo: Journal article
Palavras-chave: RP-HPLC; Validation; AZT; Pharmacokinetics; Intranasal delivery.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300010

Utilization of the cropgro-soybean model to estimate yield loss caused by Asian rust in cultivars with different cycle
Provedor de dados: Bragantia
Autores: Rodrigues,Rafael de Ávila; Pedrini,João Eduardo; Fraisse,Clyde William; Fernandes,José Maurício Cunha; Justino,Flávio Barbosa; Heinemann,Alexandre Bryan; Costa,Luiz Cláudio; Vale,Francisco Xavier Ribeiro do.
In recent years, crop models have increasingly been used to simulate agricultural features. The DSSAT (Decision Support System for Agrotechnology Transfer) is an important tool in modeling growth; however, one of its limitations is related to the unaccounted-for effect of diseases. Therefore, the goals of this study were to calibrate and validate the CSM CROPGRO-Soybean for the soybean cultivars M-SOY 6101 and MG/BR 46 (Conquista), analyze the performance and the effect of Asian soybean rust on these cultivars under the environmental conditions of Viçosa, Minas Gerais, Brazil. The experimental data for the evaluation, testing, and adjustment of the genetic coefficients for the cultivars, M-SOY 6101 and MG/BR 46 (Conquista), were obtained during the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DSSAT; Calibration; Validation; Modeling; Rust epidemics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052012000200021

Analytical validation of LAL kinetic assay for detection and quantification of endotoxins in measles's vaccine diluents
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Guimarães,Rosane Cuber; Leal,Alaide Aline Xavier.
Method validation is the process used to confirm that the analytical procedures employed for a specific test is able to produce reliable and replicable results. An analytical method should be conducted in order to demonstrate that it is suitable for its intended use [1]. The aim of this present work is to demonstrate and evaluate the suitability of method validation. The analytical validation discussed herein was conducted by prescribed protocol, using 3 different batches of measles's vaccine diluents. All of the stipulated validation parameters: linearity, repeatability, reproducibility (intermediate precision) and accuracy were met. Additionally, this study demonstrated the method's capability for the determining endotoxin levels in measles's vaccine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioanalytical method; Endotoxin; Validation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000200010

Translation into Brazilian Portuguese, cultural adaptation and evaluation of the reliability of the Disabilities of the Arm, Shoulder and Hand Questionnaire
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Orfale,A.G.; Araújo,P.M.P.; Ferraz,M.B.; Natour,J.
The objective of the present study was to translate, adapt and validate a Brazilian Portuguese version of the Disabilities of the Arm, Shoulder and Hand (DASH) Questionnaire. The study was carried out in two steps. The first was to translate the DASH into Portuguese and to perform cultural adaptation and the second involved the determination of the reliability and validity of the DASH for the Brazilian population. For this purpose, 65 rheumatoid arthritis patients of either sex (according to the classification criteria of the American College of Rheumatology), ranging in age from 18 to 60 years and presenting no other diseases involving the upper limbs, were interviewed. The patients were selected consecutively at the rheumatology outpatient clinic of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disabilities of the Arm; Shoulder and Hand (DASH); Translation into Brazilian Portuguese; Disability; Questionnaire; Arthritis; Upper limb; Validation; Arm.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200018

Development and validation of an analytical method using High Performance Liquid Chromatography (HPLC) to determine ethyl butylacetylaminopropionate in topical repellent formulations
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Pinto,Isadora Cabral; Cerqueira-Coutinho,Cristal; Freitas,Zaida Maria Faria de; Santos,Elisabete Pereira dos; Carmo,Flávia Almada do; Ricci Junior,Eduardo.
ABSTRACT Diseases caused by insects are frequent in poor countries, leading to epidemic scenarios in urban areas; e.g., Dengue, Zika and Chikungunya. For this reason, the development of a safe and efficient topical formulation is essential. Ethyl butylacetylaminopropionate (EB) is a mosquito repellent developed by Merck, which is used in products for adults, children and especially babies, due to its low allergenic potential. The aim of this work was to validate an analytical methodology to quantify EB in a new poloxamer-based formulation by high-performance liquid chromatography (HPLC). The quantification methodology was performed at 40 ºC using a Kromasil reverse-phase column (C18), with the dimensions of 250 x 4.6 mm. The mobile phase was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethyl butylacetylaminopropionate (EB)/quantification; Ethyl butylacetylaminopropionate (EB)/topical formulations; Analytical method; Validation; Repellents; Prophylaxis; High Performance Liquid Chromatography.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200613

RP-HPLC method development and validation for estimation of rivaroxaban in pharmaceutical dosage forms
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Çelebier,Mustafa; Reçber,Tuba; Koçak,Engin; Altinöz,Sacide.
Rivaroxaban, an anti-clotting medication, acts at a crucial point in the blood-clotting process and stops the formation of blood clots. In this study, RP-HPLC method was developed for the determination of rivaroxaban in tablets (Xarelto® (10 mg)). Phenomenex Luna 5 µm C18 100 Å LC Column (250 x 4.6 mm) was used at 40 ºC. Isocratic elution was performed with ACN:Water (55:45 v/v) mixture. The flow rate was 1.2 mL min-1 and UV detection was at 249 nm. Internal standard (Caffeine) and rivaroxaban were eluted within 2.21 and 3.37 minutes, respectively. The developed method was validated according to the ICH guidelines and found to be linear within the range 0.005 - 40.0 µg mL-1. The method was accurate, precise, robust and rapid. Thus, it was applied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPLC; Rivaroxaban; Validation; System suitability; Stability-indicating; Pharmaceutical dosage form.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200018

Analytical lifecycle management oriented development and validation of a new stability-indicating ultra-fast liquid chromatographic method: case study of Glycopyrrolate
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Panda,Sagar Suman; Kumar,B. V. V. Ravi; Swain,Suchismita.
A new stability-indicating liquid chromatographic method was developed and validated for the estimation of glycopyrrolate in pharmaceutical formulations. A contemporary approach to analytical life-cycle management was followed to develop a robust and reliable chromatographic method. Scouted method variables such as % methanol, the strength of tetra butyl ammonium hydrogen sulfate and mobile phase flow rate were optimized using the design of experiment approach and their effect on critical quality attributes was studied. The critical quality attributes viz. retention time, theoretical plate count and symmetry factor were highly influenced by the three critical method variables. Optimum chromatography was attained on a C-18 column with a mobile phase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycopyrrolate; Stability-indicating; Liquid chromatography; Life cycle management; Validation.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100605

Development and validation of a microbiological assay for determination of chlorhexidine digluconate in aqueous solution
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Fiorentino,Flávia Angélica Másquio; Corrêa,Marcos Antonio; Salgado,Hérida Regina Nunes.
Chlorhexidine (CHX) is a broad-spectrum antiseptic that is used in many topical pharmaceutical formulations. Because there is no official microbiological assay reported in the literature that is used to quantify CHX, this paper reports the development and validation of a simple, sensitive, accurate and reproducible agar diffusion method for the dosage of chlorhexidine digluconate (CHX-D) in an aqueous solution. The assay is based on the inhibitory effect of CHX-D upon the strain of Staphylococcus aureus ATCC 25923, which is used as the test microorganism. The design 3x3 parallel-line model was used. The results were treated statistically by analysis of variance (ANOVA), and they were excellent in terms of linearity (r = 0.9999), presenting a significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agar diffusion; Antiseptic; Chlorhexidine; Microbiological assay; Quality control; Validation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200017

Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Razzaq,Syed Naeem; Ashfaq,Muhammad; Khan,Islam Ullah; Mariam,Irfana; Razzaq,Syed Saleem; Mustafa,Ghulam; Zubair,Muhammad.
Abstract In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gatifloxacin; Dexamethasone; High performance liquid chromatography; Validation; Plasma; Degradation products.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100607

Development of a new HPLC method for in vitro and in vivo studies of haloperidol in solid lipid nanoparticles
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Yasir,Mohd; Sara,Udai Vir Singh; Som,Iti.
ABSTRACT A simple and sensitive HPLC method was developed and validated for the quantification of haloperidol in solid lipid nanoparticles (SLNs). The developed method was used for detection of shelf life of haloperidol in SLNs. Calibration curve of haloperidol was also constructed in rat plasma using loratidine as internal standard. In vivo studies were performed on rats and concentration of haloperidol in brain and blood was measured for the determination of various pharmacokinetic and hence brain targeting parameters. Chromatogram separation was achieved using C18 column as stationary phase. The mobile phase consisted of 100 mM/L potassium dihydrogen phosphate-acetonitrile-TEA (10:90:0.1, v/v/v) and the pH was adjusted with o-phosphoric acid to 3.5....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haloperidol/quantification; Haloperidol/pharmacokinetic; Haloperidol/in vitro study; Haloperidol/in vivo study; High performance liquid chromatography/solid lipid nanoparticles; Plasma; Validation; Brain targeting.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200623

HPLC method development for the simultaneous analysis of amlodipine and valsartan in combined dosage forms and in vitro dissolution studies
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Çelebier,Mustafa; Kaynak,Mustafa Sinan; Altınöz,Sacide; Sahin,Selma.
A simple, rapid and reproducible HPLC method was developed for the simultaneous determination of amlodipine and valsartan in their combined dosage forms, and for drug dissolution studies. A C18 column (ODS 2, 10 μm, 200 x 4.6 mm) and a mobile phase of phosphate buffer (pH 3.6 , 0.01 mol L-1):acetonitrile: methanol (46:44:10 v/v/v) mixture were used for separation and quantification. Analyses were run at a flow-rate of 1 mL min-1 and at ambient temperature. The injection volume was 20 μL and the ultraviolet detector was set at 240 nm. Under these conditions, amlodipine and valsartan were eluted at 7.1 min and 3.4 min, respectively. Total run time was shorter than 9 min. The developed method was validated according to the literature and found to be linear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amlodipine besylate; Valsartan; High Performance Liquid Chromatography; Method Development; Validation; Dissolution Study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400018

Validation of spectrophotometric method to detect and quantify nitrite in ham pate
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Reis,Patrícia Barros; Ramos,Rodrigo Mauro; Souza,Leonardo Francisco de; Cançado,Silvana de Vasconcelos.
The objective of this work was to validate the spectrophotometric method to detect and quantify nitrite using ham pate as a source. The validated analytical conditions were 540 nm wavelength and the samples reading between 40 to 70 minutes after addition of coloring agents. The tested performance criteria were: linearity, matrix effect, selectivity, detection and quantification limit, accuracy, precision and robustness. As the results have obtained linearity in the studied zone (0.125 to 3 g/mL) and matrix effect was not observed, there was not any interference from ascorbic acid, but interference occurred from sodium eritorbate, in concentrations above 2.5 mg/100g. The detection limit was defined as 12.5 mg of nitrite/kg for pate and the quantification...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Validation; Nitrite; Spectrophotometric method; Ham pate.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100006

Determining the site index of Teak (Tectona grandis L.) plantations in Tabasco, Mexico
Provedor de dados: Ciencia e Investigación Agraria, Revista Latinoamericana en Ciencias de la Agricultura
Autores: Minoche,Djhon; Herrero,Celia; Dominguez-Dominguez,Marivel; Martinez-Zurimendi,Pablo.
Abstract Forest stand productivity is defined as the quantitative estimation of a specific area's potential to produce biomass over a determined period of time. The site index has been the predominant method used to evaluate forest stand productivity. Teak is one of the most accepted species within the international timber market due to the physical and aesthetic qualities of this wood. The aim of this study was to determine the site index of teak plantations. The study was conducted in teak plantations of Tabasco. Data were obtained from a network of 10 plantations consisting of 35 plots measured over four successive inventories (2003 to 2006). The data were fitted to five models, of which four were based on proposed finite difference equations and a...
Tipo: Journal article
Palavras-chave: Finite difference equations; Forest productivity; Inventory; Non-integrated function; Validation.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000200154

Prediction of soil organic matter and clay contents by near-infrared spectroscopy - NIRS
Provedor de dados: Ciência Rural
Autores: Lazzaretti,Bruno Pedro; Silva,Leandro Souza da; Drescher,Gerson Laerson; Dotto,André Carnieletto; Britzke,Darines; Nörnberg,José Laerte.
ABSTRACT: Among the soil constituents, special attention is given to soil organic matter (SOM) and clay contents, since, among other aspects, they are key factors to nutrient retention and soil aggregates formation, which directly affect the crop production potential. The methods commonly used for the quantification of these constituents have some disadvantages, such as the use of chemical reactants and waste generation. An alternative to these methods is the near-infrared spectroscopy (NIRS) technique. The aim of this research is to evaluate models for SOM and clay quantification in soil samples using spectral data by NIRS. A set (n = 400) of soil samples previously analyzed by traditional methods were used to generate a NIRS calibration curve. The clay...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calibration; Validation; Mathematical models; Spectral pretreatment.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000100251

Development of a scale to evaluate mobility in dogs
Provedor de dados: Ciência Rural
Autores: Gonçalves,Luísa Carneiro Vasconcelos Basto; Simões,Ana Daniela Gomes Araújo; Millis,Darryl Lynn; Matos,Augusto José Ferreira de.
ABSTRACT: The purpose of this study was to develop and to assess the psychometric characteristics of a mobility scale for dogs. The original ten questions were reduced using validation process. One hundred and twenty three dog owners were invited to answer the questionnaire. Internal consistency, factor analysis, floor and ceiling effect and construct validity were studied. Good internal consistency (Cronbach's Alpha=0.854) was determined with two items eliminated. The instrument comprises 8 final questions, each of which has five possible answers (never, rarely, sometimes, often and always) scored between 0 and 4 or between 4 and 0 (for the items with inverse score). Three hypotheses proposed for the construct validity were verified: 1) gender does not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine; Mobility; Psychometric methodology; Questionnaire; Validation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202210