Consulta: subjectFacets:"Vibrio"
Registros recuperados: 50
Data/hora: 17/06/2024 10:04:26

Expression, tissue localization and synergy of antimicrobial peptides and proteins in the immune response of the oyster Crassostrea gigas
Provedor de dados: 5
Autores: Schmitt, Paulina; De Lorgeril, Julien; Gueguen, Yannick; Destoumieux-garzon, Delphine; Bachere, Evelyne.
Diverse families of antimicrobial peptides and proteins have been described in oysters. We investigated here how antimicrobials are involved in the immune response against a pathogenic strain of Vibrio splendidus. Oyster antimicrobials were shown to display a wide variety of expression profiles in hemocyte populations and tissues. Oyster defensins are constitutively expressed in specific tissues such as mantle (Cg-Defm) or hemocytes (Cg-Defhs), while Cg-BPI is inducible and Cg-Prp appears down-regulated in hemocytes upon infection. The migratory behavior of hemocytes that express the different antimicrobials was found to be involved in the oyster response to a pathogenic Vibrio infection. Indeed, it contributes to colocalize several antimicrobials that...
Tipo: Text
Palavras-chave: Pacific oyster; Bivalve mollusk; Innate immunity; Bactericidal/permeability-increasing protein; Proline-rich peptide; Defensin; Vibrio.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00093/20382/18267.pdf

Characterization of biofilm extracts from two marine bacteria
Provedor de dados: 5
Autores: Passerini, Delphine; Fecamp, Florian; Marchand, Laetitia; Kolypczuk, Laetitia; Bonnetot, Sandrine; Sinquin, Corinne; Verrez-bagnis, Veronique; Hervio Heath, Dominique; Colliec Jouault, Sylvia; Delbarre Ladrat, Christine.
In the marine environment, biofilm formation is an important lifestyle for microorganisms. A biofilm is comprised of cells embedded in an extracellular matrix that holds them close together and keeps the biofilm attached to the colonized surface. This predominant lifestyle and its main regulation pathway, namely quorum-sensing (QS), have been shown to induce specific bioactive metabolites. In this study, we investigated the biofilm formation by two marine bacteria belonging to the Vibrio species to discover potentially innovative bioactive compounds. We proposed a protocol to isolate biofilm extracts, to analyze their biochemical composition, and to compare them to planktonic cell extracts. Cells were grown attached to a plastic surface; extracts were...
Tipo: Text
Palavras-chave: Vibrio; Biofilm; Polysaccharide; Quorum-sensing; Activity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00592/70381/68461.pdf

Real-time PCR assay for rapid detection and quantification of Vibrio aestuarianus in oyster and seawater: A useful tool for epidemiologic studies
Provedor de dados: 5
Autores: Saulnier, Denis; De Decker, Sophie; Haffner, Philippe.
Because Vibrio aestuarianus is known to cause serious infections in Pacific oyster Crassostrea gigas, a real-time PCR assay was developed targeting the dnaJ gene of this bacterium. Only V. aestuarianus strains isolated from C. gigas mortality events in different geographic areas and the reference strain tested positive, whereas no amplification products was obtained with type strains belonging to 23 other species of Vibrio. Sensitivity and reproducibility of the method were assessed using either seawater or oyster homogenate samples spiked with one V aestuarianus strain. All these samples were stored at -20 degrees C in order to mimic retrospective or grouped natural sample analysis without quantification bias due to prolonged freezing. Analysis of...
Tipo: Text
Palavras-chave: V. aestuarianus; Vibrio; Taqman; Real time PCR; Pathogen; Oyster; Crassostrea gigas.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6447.pdf

"Summer Syndrome" in Litopenaeus stylirostris in New Caledonia: Pathology and epidemiology of the etiological agent, Vibrio nigripulchritudo
Provedor de dados: 5
Autores: Goarant, Cyrille; Ansquer, Dominique; Herlin, Jose; Domalain, David; Imbert, Frederic; De Decker, Sophie.
The Summer Syndrome is a new shrimp disease that has been affecting a shrimp growout farm in New Caledonia since end of 1997. It was recognized to be caused by a systemic vibriosis due to Vibrio nigripulchritudo. This new disease turned out almost immediately enzootic in the shrimp farm involved and has affected all its crops ever since. Since the year 2000, V. nigripulchritudo strains have been found in several shrimp farms, although Summer Syndrome is still limited to one particular area, affecting, since 2003, two adjoining farms. As part of a multidisciplinary research program, a high-frequency survey was carried out during the summer 2002-2003 in two shrimp farms: one affected farm and another one in which pathogenic strains could be isolated but...
Tipo: Text
Palavras-chave: Epidemiology; Vibrio; Diseases and their control; Shrimp.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1110.pdf

A MALDI-TOF MS database for fast identification of Vibrio spp. potentially pathogenic to marine mollusks
Provedor de dados: 5
Autores: Moussa, M.; Cauvin, E.; Le Piouffle, A.; Lucas, O.; Bidault, Adeline; Paillard, Christine; Benoit, F.; Thuillier, B.; Treilles, M.; Travers, Marie-agnes; Garcia, Celine.
In mollusk aquaculture, a large number of Vibrio species are considered major pathogens. Conventional methods based on DNA amplification and sequencing used to accurately identify Vibrio species are unsuitable for monitoring programs because they are time-consuming and expensive. The aim of this study was, therefore, to develop the MALDI-TOF MS method in order to establish a rapid identification technique for a large panel of Vibrio species. We created the EnviBase containing 120 main spectra projections (MSP) of the Vibrio species that are potentially responsible for mollusk diseases, comprising 25 species: V. aestuarianus, V. cortegadensis, V. tapetis and species belonging to the Coralliilyticus, Harveyi, Mediterranei, and Orientalis clades. Each MSP was...
Tipo: Text
Palavras-chave: Vibrio; MALDI-TOF MS; Database; Marine mollusk pathogens.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00680/79224/81732.pdf

Quantification of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae in French Mediterranean coastal lagoons
Provedor de dados: 5
Autores: Cantet, Franck; Hervio-heath, Dominique; Caro, A; Le Mennec, Cecile; Monteil, Caroline; Quemere, Catherine; Jolivet-gougeon, Anne; Colwell, Rita R.; Monfort, Patrick.
Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae are human pathogens. Little is known about these Vibrio spp. in the coastal lagoons of France. The purpose of this study was to investigate their incidence in water, shellfish and sediment of three French Mediterranean coastal lagoons using the most probable number-polymerase chain reaction (MPN-PCR). In summer, the total number of V. parahaemolyticus in water, sediment, mussels and clams collected from the three lagoons varied from 1 to &gt;1.1 x 10.3 MPN/l, 0.09 to 1.1 x 10.3 MPN/ml, 9 to 210 MPN/g and 1.5 to 2.1 MPN/g, respectively. In winter, all samples except mussels contained V. parahaemolyticus, but at very low concentrations. Pathogenic (tdh- or trh2-positive) V. parahaemolyticus were...
Tipo: Text
Palavras-chave: Vibrio; Lagoons; Shellfish; Water; Sediment; Human pathogen.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00148/25890/23889.pdf

Modulations of the interactions between pacific cupped oyster Crassostrea gigas and Vibrio according to bacterial virulence and to genetic and physiological status of the host : Methodology and first results
Provedor de dados: 5
Autores: De Decker, Sophie; Normand, Julien; Duperthuy, Marylise; Boudry, Pierre; Saulnier, Denis.
Rearing of Crassostrea gigas is the most economically important aquaculture activity in France. Bacteria belonging to Vibrio genus constitute one of the most abundant bacterial group in marine ecosystems. Two Vibrio species, V. splendidus and V. aestuarianus, were associated with many cases of mortality events in reared C. gigas spat and juvenile oysters, commonly during summer. This summer mortality syndrome has been extensively documented as the result of complex interactions between pathogens, host and environmental conditions. This work aims to study the Vibrio-host interactions and their modulations according to the virulence of pathogens and the genetic and/or physiological parameters of the host. These factors will be characterized by in vitro and...
Tipo: Text
Palavras-chave: Genetic; Pathogens; Interaction; Vibrio; Crassostrea gigas; Oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3430.pdf

Bacterial diseases in marine bivalves
Provedor de dados: 5
Autores: Travers, Marie-agnes; Miller, Katherine Boettcher; Roque, Ana; Friedman, Carolyn S.
Bivalve aquaculture is seriously affected by many bacterial pathogens that cause high losses in hatcheries as well as in natural beds. A number of Vibrio species, but also members of the genera Nocardia and Roseovarius, are considered important pathogens in aquaculture. The present work provides an updated overview of main diseases and implicated bacterial species affecting bivalves. This review focuses on aetiological agents, their diversity and virulence factors, the diagnostic methods available as well as information on the dynamics of the host-parasite relationship.
Tipo: Text
Palavras-chave: Vibrio; Nocardia; Roseovarius; Rickettsia; Pathogenesis; Diagnostic.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00274/38532/37049.pdf

Complex Relationships between the Blue Pigment Marennine and Marine Bacteria of the Genus Vibrio
Provedor de dados: 5
Autores: Falaise, Charlotte; James, Adele; Travers, Marie-agnes; Zanella, Marie; Badawi, Myriam; Mouget, Jean-luc.
Marennine, the water-soluble blue pigment produced by the marine diatom Haslea ostrearia, is known to display antibacterial activities. Previous studies have demonstrated a prophylactic effect of marennine on bivalve larvae challenged with a pathogenic Vibrio splendidus, suggesting that the blue Haslea is a good candidate for applications in aquaculture as a source of a natural antimicrobial agent. Indeed, the genus Vibrio is ubiquitous in aquaculture ecosystems, and regular events of pathogenic invasion cause some of the biggest losses worldwide. To better characterize the effects of marennine on Vibrios, a panel of 30 Vibrio strains belonging to 10 different species was tested, including bivalve pathogenic species (e.g., Vibrio crassostreae and Vibrio...
Tipo: Text
Palavras-chave: Antibacterial activity; Diauxie; Haslea; Hormesis; Marennine; Vibrio.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00484/59598/62602.pdf

Development of an antiseptic procedure to improve retention rates during the grafting process of black-lip oyster, Pinctada margaritifera
Provedor de dados: 5
Autores: Cochennec, Nathalie; Haffner, Philippe; Levy, Peva; Saulnier, Denis; Fougerouse, A.; Langy, Sandra.
In French Polynesia, black pearl cultivation now takes place on about 30 of the 118 atolls or islands. This activity has largely replaced copra, coconut oil and fishing as a source of income. Around 1,000 pearl farms, generally small ones of less than 5 ha, are registered. The pearl farms are mainly owned by families. In 1998, about 7,000 persons were employed in theentire pearl industry, from production to retailing. In value, this activity represents the first exportation of French Polynesian products. From 1988 to date, exports increased exponentially before leveling. Starting with less than two kilograms in 1972, exports have now stabilized at Il tons of "black" pearls.
Tipo: Text
Palavras-chave: Vibrio; Microbiology; Grafting process; Pinctada margaritifera.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-4928.PDF

Vibrio crassostreae sp nov., isolated from the haemolymph of oysters (Crassostrea gigas)
Provedor de dados: 5
Autores: Faury, Nicole; Saulnier, Denis; Thompson, F; Gay, Melanie; Swings, J; Leroux, Frederique.
Polyphasic analysis of five new Vibrio isolates originating from the haernolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. gyrB phylogenetic analysis, fluorescent amplified-fragment length polymorphism (FAFLP) fingerprinting and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed to accommodate these isolates in a novel species, Vibrio crassostreae sp. nov. (type strain LGP 7(T) =LMG 22240(T)=Clp 108327(T)). Phenotypic and chemotaxonomic features that differentiate V. crassostreae from other known Vibrio species include arginine...
Tipo: Text
Palavras-chave: Identification; Vibrio; Crasssotrea gigas; Ostreiculture.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-591.pdf

Selection of lactic acid bacteria as candidate probiotics and in vivo test on Artemia nauplii
Provedor de dados: 5
Autores: Lamari, Faouzi; Sadok, Khouadja; Bakhrouf, Amina; Gatesoupe, Joel.
Lactic acid bacteria (LAB) were isolated from rotifer cultures in a marine hatchery to search for potential probiotics for marine animals. Fifteen strains were first selected among a total of 55, according to antibacterial activity against Vibrio sp. Among eight strains identified as Lactobacillus casei, four were highly adhesive, suggesting some ability for surface colonization. The other strains were identified as Lactobacillus plantarum, Lactobacillus dextrinicus, and Leuconostoc sp. To validate probiotic potential, Artemia were challenged against pathogenic Vibrio alginolyticus, with or without one of six selected LAB strains. The six strains protected Artemia against the pathogen, to some extent on condition that nutrient enrichment was provided. La....
Tipo: Text
Palavras-chave: 16S rDNA; Antagonism; Artemia; Lactic acid bacteria; Vibrio.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00154/26511/24767.pdf

Oyster Farming, Temperature, and Plankton Influence the Dynamics of Pathogenic Vibrios in the Thau Lagoon
Provedor de dados: 5
Autores: Lopez-joven, Maria-carmen; Rolland, Jean-luc; Haffner, Philippe; Caro, Audrey; Roques, Cecile; Carre, Claire; Travers, Marie-agnes; Abadie, Eric; Laabir, Mohamed; Bonnet, Delphine; Destoumieux-garzon, Delphine.
Vibrio species have been associated with recurrent mass mortalities of juvenile oysters Crassostrea gigas threatening oyster farming worldwide. However, knowledge of the ecology of pathogens in affected oyster farming areas remains scarce. Specifically, there are no data regarding (i) the environmental reservoirs of Vibrio populations pathogenic to oysters, (ii) the environmental factors favoring their transmission, and (iii) the influence of oyster farming on the persistence of those pathogens. This knowledge gap limits our capacity to predict and mitigate disease occurrence. To address these issues, we monitored Vibrio species potentially pathogenic to C. gigas in 2013 and 2014 in the Thau Lagoon, a major oyster farming region in the coastal French...
Tipo: Text
Palavras-chave: Vibrio; Shellfish farming; Bivalve mollusks; Mortality outbreak; Phytoplankton; Zooplankton.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00463/57479/59598.pdf

Maintenance of discrete bacterial populations in adjacent marine habitats.
Provedor de dados: 5
Autores: Robb, F; Muir, D; Davis, C.
Some mechanisms of selection which operate on marine Vibrio and Pseudomonas strains during survival in dilute environments have been examined. Mannitol uptake and utilisation systems are more efficient in the Vibrio strain compared with the Pseudomonas strain during starvation survival. A Vibrio mutant strain lacking mannitol uptake showed no greater loss of viability during starvation survival than mannitol positive strains. The Vibrio strain appears to be constitutive with respect to mannitol uptake systems, whereas the Pseudomonas strain examined is inducible. Active uptake systems for glucose, glutamate, alanine and aspartic acid were efficiently maintained during starvation survival in the Vibrio strain. Results suggest that membrane uptake systems...
Tipo: Text
Palavras-chave: Pseudomonas; Vibrio; Bacteria; Physiology; Alcohols; Food conversion; Survival; Starvation; Metabolism.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-960.pdf

Isolement et identification de vibrions halophiles d'une station experimentale de mariculture
Provedor de dados: 5
Autores: Bruni, V; Crisafi, E; Maugeri, T; Zaccone, R.
110 Vibrio strains were isolated, at various times of the year, from samples of sea-water supply, breeding-tank water and discarded fish used as food at the breeding stations. From the results obtained by the biochemical and cultural tests, it was possible to identify most of the isolated strains as Vibrio anguillarum . Strains with intermediate characteristics, above-all positive saccharose, proved to be numerous. The authors discuss the occurrence, in particular environments such as those being studied, of atypical or intermediate vibrio strains which are difficult to classify.
Tipo: Text
Palavras-chave: Vibrio; Microbiological culture; Bacteria; Food; Minced products; Fish ponds; Sea water; Identification; Marine aquaculture; Bacteria collecting devices; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-997.pdf

Sequence polymorphism-based identification and quantification of Vibrio nigripulchritudo at the species and subspecies level targeting an emerging pathogen for cultured shrimp in New Caledonia
Provedor de dados: 5
Autores: Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Merien, Fabrice.
In a previous study, we demonstrated the existence of an emerging cluster of Vibrio nigripulchritudo that proved to be associated with shrimp mortality events in New Caledonia. Using sequence polymorphisms evidenced in this previous MultiLocus Sequence Typing study, we developed two new quantitative PCR assays permitting the detection and quantification of V. nigripulchritudo at the genospecies level using SYBR Green I chemistry and at the emerging cluster level using Fluorescence Resonance Energy Transfer technology with hybridization probes.   The use of this molecular diagnostic tool evidenced the colonization of the shrimp pond ecosystem by the pathogenic cluster at least at the onset of the disease. This new tool will allow better investigation of the...
Tipo: Text
Palavras-chave: Shrimp; Cluster specific; Vibrio; Pathogen; Mariculture; Real time PCR.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2729.pdf

Genome sequence of Vibrio diabolicus and identification of the exopolysaccharide HE800 biosynthesis locus
Provedor de dados: 5
Autores: Goudenege, David; Boursicot, Vincent; Versigny, Typhaine; Bonnetot, Sandrine; Ratiskol, Jacqueline; Sinquin, Corinne; Lapointe, Gisele; Le Roux, Frederique; Delbarre-ladrat, Christine.
Vibrio diabolicus, a marine bacterium originating from deep-sea hydrothermal vents, produces the HE800 exopolysaccharide with high value for biotechnological purposes, especially for human health. Its genome was sequenced and analyzed; phylogenetic analysis using the core genome revealed V. diabolicus is close to another deep-sea Vibrio sp. (Ex25) within the Harveyi clade and Alginolyticus group. A genetic locus homologous to the syp cluster from Vibrio fischeri was demonstrated to be involved in the HE800 production. However, few genetic particularities suggest that the regulation of syp expression may be different in V. diabolicus. The presence of several types of glycosyltransferases within the locus indicates a capacity to generate diversity in the...
Tipo: Text
Palavras-chave: Genome; Vibrio; Exopolysaccharide; Biosynthesis.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00241/35244/33892.pdf

Symbiodinium     clades A and D differentially predispose     Acropora cytherea     to disease and     Vibrio     spp. colonization
Provedor de dados: 5
Autores: Rouze, Heloise; Lecellier, Gael; Saulnier, Denis; Berteaux-lecellier, Veronique.
Coral disease outbreaks have increased over the last three decades, but their causal agents remain mostly unclear (e.g., bacteria, viruses, fungi, protists). This study details a 14-month-long survey of coral colonies in which observations of the development of disease was observed in nearly half of the sampled colonies. A bimonthly qPCR method was used to quantitatively and qualitatively evaluate Symbiodinium assemblages of tagged colonies, and to detect the presence of Vibrio spp. Firstly, our data showed that predisposition to disease development in general, and, more specifically, infection by Vibrio spp. in Acropora cytherea depended on which clades of Symbiodinium were harbored. In both cases, harboring clade D rather than A was beneficial to the...
Tipo: Text
Palavras-chave: Coral disease; Dynamic; Resistance; Symbiodinium assemblages; Vibrio.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00307/41848/41080.pdf

Utilisation de bactéries lactiques probiotiques pour prémunir les poissons d'élevage contre des vibrions pathogènes
Provedor de dados: 5
Autores: Lamari, Faouzi.
Diseases cause major production losses in fish farms. Bacteria of the genus Vibrio are known as opportunistic pathogens, and have been associated with mass-mortality episodes. To cope with these disease outbreaks, fish farmers often resort to the use of antibiotics. However, preventive overuse has led to the emergence of resistant strains and frequent treatment failures. Probiotic lactic acid bacteria are increasingly used to improve the quality of seed production in aquaculture, while limiting antibiotic treatments. Microbial strains must fulfil several criteria to be evaluated as potential probiotics.In a first step, we selected and characterized 55 strains of lactic acid bacteria isolated from fish hatchery. The first selection criterion focused on the...
Tipo: Text
Palavras-chave: Probiotiques; Microflore intestinale; Réponse immunitaire; Stress oxydatif; Malformations vertébrales; Ossification; Oxidative stress; Vertebral malformations; Dicentrarchus labrax; Artemia; Vibrio; Probiotic; Intestinal microflora; Immune response.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00498/60943/64336.pdf

Etude de la virulence des Vibrio pathogènes en relation avec la variabilité de la réponse chez l'uître creuse
Provedor de dados: 5
Autores: De Decker, Sophie.
The bacteria of the Vibrio genus are one of the most abundant groups in the aquatic ecosystems. Two species of vibrios, Vibrio splendidus and V. Aestuarianus, have been associated, in some cases, to mortality in flat oyster Crassostrea gigas farms, most of the time during the summer period. This doctoral thesis studies the Vibrio-host interaction and their modulations according to the host's pathogenic virulence and its genetic, physiologic and immunitary parameters. These factors will be characterised by in vitro and in vivo approaches (experimental infection). The characterization of contrasted genetic families according to their sensitivity to these vibrios will enable the implementation of new improvement strategies in oyster farms (prophylactic...
Tipo: Text
Palavras-chave: Immunologie; Physiologie; Paramètres génétiques; Virulence; Oyster; Crassostrea gigas; Pathogène; Vibrio.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3431.pdf

Comparative genomics of pathogenic lineages of Vibrio nigripulchritudo identifies virulence-associated traits
Provedor de dados: 5
Autores: Goudenege, David; Labreuche, Yannick; Krin, Evelyne; Ansquer, Dominique; Mangenot, Sophie; Calteau, Alexandra; Medigue, Claudine; Mazel, Didier; Polz, Martin F.; Le Roux, Frederique.
Vibrio nigripulchritudo is an emerging pathogen of farmed shrimp in New Caledonia and other regions in the Indo-Pacific. The molecular determinants of V. nigripulchritudo pathogenicity are unknown; however, molecular epidemiological studies have suggested that pathogenicity is linked to particular lineages. Here, we performed high-throughput sequencing-based comparative genome analysis of 16 V. nigripulchritudo strains to explore the genomic diversity and evolutionary history of pathogen-containing lineages and to identify pathogen-specific genetic elements. Our phylogenetic analysis revealed three pathogen-containing V. nigripulchritudo clades, including two clades previously identified from New Caledonia and one novel clade comprising putatively...
Tipo: Text
Palavras-chave: Horizontal gene transfer; Nigritoxin; Phylogeny; Shrimp; Virulence; Vibrio.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00160/27117/28369.pdf

Probiotic effect of FLOC on Vibrios in the pacific white shrimp Litopenaeus Vannamei
Provedor de dados: 5
Autores: Aguilera-rivera, Diana; Prieto-davo, Alejandra; Escalante, Karla; Chavez, Cristina; Cuzon, Gerard; Gaxiola, Gabriela.
The advantages of FLOC over clear water (CW) in rearing juvenile L. vannamei and its effects on Vibrio communities were evaluated. Survival rate in FLOC and clear water were recorded and a probiotic was tested under both conditions. Daily growth rate (DGR) was higher in FLOC (p &lt; 0.05) than in CW. Survival in each system increased significantly when a probiotic was included in the diet (p &lt; 0.05). The Vibrionaceae community from the hepatopancreas (HP) and the culture medium did not differ between the two culture media. Nevertheless, a novel group of Vibrio strains was found to be unique to FLOC. No high level of lesions was observed in shrimp tissues from the FLOC + probiotic treatment; it suggests that the probiotic contributed to homeostasis and...
Tipo: Text
Palavras-chave: FLOC; Probiotic; L. vannamei; Disease; Vibrio.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00173/28379/26694.pdf

Bacteries associees aux production de Brachionus plicatilis
Provedor de dados: 5
Autores: Nicolas, Jean-louis; Joubert, M.
Bacteria associated with semi-continuous culture of Brachionus plicatilis , which is used as the first food of marine fish larvae, were counted on Zobell 2216 E medium and identified. The numbers of bacteria were very high in both Brachionus (about 10 super(11) bact. per ml of digestive content) and in water (from 1.6.10 super(6) to 4.7.10 super(7) bact. per ml). The cultures were studied at driving a time when mortalities occurred frequently. The introduction of a Pseudomonas) phenon at a proportion of about 50% to the dominant microflora persisted in time and was little influenced by diet. It has been the cause of the repression of the Vibrio, Aeromonas group which was found in an unusually small proportion. It induced a lack of microflora diversity and...
Tipo: Text
Palavras-chave: Brachionus plicatilis; Rotifera; Pseudomonas; Aeromonas; Vibrio; Nutrition; Pathology; Fish culture; Fish larvae; Food organisms; Bacteria; Continuous culture; Ecological associations; Zooplankton culture.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-994.pdf

Stylicins, a new family of antimicrobial peptides from the Pacific blue shrimp Litopenaeus stylirostris
Provedor de dados: 5
Autores: Rolland, Jean-luc; Abdelouahab, Mahdia; Dupont, J.; Lefevre, F.; Bachere, Evelyne; Romestand, Bernard.
The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenoeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pl=5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containing 13 cysteine residues. The recombinant Ls-Stylicin1 has been isolated in both monomeric and dimeric forms. Both display strong antifungal activity against Fusarium oxysporum (1.25 mu M &lt; MIC &lt;2.5 mu M), a pathogenic fungus of shrimp, but lower antimicrobial activity against Gram () bacteria, Vibrio sp. (40 mu M &lt; MIC &lt;80 mu M). However, rLs-Stylicin1 is able to agglutinate Vibrio pennaeicidae in vitro in agreement with its potent...
Tipo: Text
Palavras-chave: Ls-Stylicin 1; Peptide; Shrimp; Antifungal; Agglutination; Vibrio.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00003/11387/8094.pdf

Effect of probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress of Litopenaeus stylirostris under Vibrio nigripulchritudo challenge
Provedor de dados: 5
Autores: Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet.
Antioxidant defences and induced oxidative stress tissue damage of the blue shrimp Litopenaeus stylirostris, under challenge with Vibrio nigriputchritudo, were investigated for a 72-h period. For this purpose, L stylirostris were first infected by immersion with pathogenic V. nigripulchritudo strain SFn1 and then antioxidant defences: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx), Total antioxidant status (TAS), glutathiones and induced tissue damage (MDA and carbonyl proteins) were determined in the digestive gland at 0, 12, 24, 48 and 72 h post-infection (h.p.i.). In the meantime, TAS was also measured in the blood. Infection level of the shrimps during the challenge was followed by determining V. nigripulchritudo prevalence...
Tipo: Text
Palavras-chave: Probiotic; Pediococcus acidilactici; Shrimp; Oxidative stress; Antioxidant status; Vibrio; Immersion challenge.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00003/11419/7984.pdf

Eutrophication in a tropical pond: Understanding the bacterioplankton and phytoplankton dynamics during a vibriosis outbreak using flow cytometric analyses
Provedor de dados: 5
Autores: Lucas, Ronan; Courties, C.; Herbland, Alain; Goulletquer, Philippe; Marteau, Anne-laure; Lemonnier, Hugues.
In tropical shrimp ponds, the increasing of feed input, concomitantly with the stocking shrimp biomass, induces an eutrophication of the ecosystem. Although difficult to maintain, its stability is required to guarantee the success of the culture. A 110-day period of phytoplankton and bacterioplankton stock and dynamics in an earthen pond (1.2 ha area, 1 m depth) was monitored using flow cytometry to provide baseline information on community characteristics and ecosystem instability. Seven autotrophic cell types were identified over the whole sampling period. Prokaryotic cells included Synechococcus sp., a group named UNK which presented an atypical new flow cytometric signature and picoeukaryotes (PEUK). Nanophytoplankton cells were represented by 4...
Tipo: Text
Palavras-chave: Tropical shrimp aquaculture; Ecosystem shift; Phytoplankton; Bacterioplankton; Vibrio; Flow cytometry.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00029/14068/11312.pdf

Recherche et identification de bactéries pathogènes au sein d'une écloserie nurserie d'huître creuse, Crassostrea gigas, en relation avec la mortalité estivale du naissain
Provedor de dados: 5
Autores: Waechter, Magali.
Summer mortality has been observed on oyster spat Crassostrea gigas since 1991 on french coasts. In our study, we deseribed symptoms observed during the mortality, especially adductor muscle weakness and we isolated strains on Zobell medium and TCBS from moribund spat. Strains isolated during a high mortality (60 %) were selected by experimental pathology on spat. Inoculation by bath led us to select a pathogenic strain (TNEMF6) for oyster spat. Phenotypic identification, phylogenetic study on 16S rDNA and quantitative DNA/DNA hybridisation showed that this strain belongs to Vibrio splendidus biovar II. Phenotyping and ribotyping with strains isolated during the study showed that only TNNIII7 and TNMB5 are similar to the pathogenic strain TNEMF6....
Tipo: Text
Palavras-chave: Mortalité estivale; Naissain; Crassostrea gigas; Ecloserie; Nursery; France; Bactériologie; Bactérie; Vibrio; Vibrio splendidus biovar II; Summer mortality; Oyster spat; Crassostrea gigas; Hatchery; Nursery; France; Bacteriology; Bacteria; Vibrio; Vibrio splendidus biovar II.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00211/32226/30682.pdf

Responses of diploid and triploid Pacific oysters Crassostrea gigas to Vibrio infection in relation to their reproductive status
Provedor de dados: 5
Autores: De Decker, Sophie; Normand, Julien; Saulnier, Denis; Pernet, Fabrice; Castagnet, Sophie; Boudry, Pierre.
Several Vibrio species are known to be pathogenic to the Pacific oyster Crassostrea gigas. Survival varies according to pathogen exposure and high mortality events usually occur in summer during gametogenesis. In order to study the effects of gametogenetic status and ploidy (a factor known to affect reproduction allocation in oysters) on vibriosis survival, we conducted two successive experiments. Our results demonstrate that a common bath challenge with pathogenic Vibrio splendidus and Vibrio aestuarianus on a mixture of mature, spawning and non-mature oysters can lead to significant mortality. Previous bath challenges, which were done using only non-mature oysters, had not produced mortality. Immunohisto-chemical analyses showed the affinity of Vibrio...
Tipo: Text
Palavras-chave: Experimental vibriosis; Crassostrea gigas; Vibrio; Survival distribution function; Triploidy; Reproductive effort.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00030/14108/11368.pdf

Comparative study of bactrial infections responsible for mass mortality in penaeid shrimp hatcheries of the Pacific zone
Provedor de dados: 5
Autores: Le Groumellec, M.; Haffner, Philippe; Martin, B.; Martin, C.
Mass mortality associated with luminescence has been dbserved in sorne Thai and Ecuadorian penaeid shrimp hatcheries in recent years. Samples collected from hatcheries in these two different areas revealed luminescent sucrose-negative Vibrio strains in larVal rearing tanks where mortality occurred. Pathogenicity trials were carried out to identify the causative agents of mortality and to study the course of infection . . Results showed that strains isolated from Thai hatclleries were more virulent than Ecuadorian strains when tested on different larval stages of Penaeus monodon. The virulence of these strains was also estimated. Trials on other species ofpenaeid shrimp demonstrated the specifie action of Thai strains. Drug sensitivity patterns revealed a...
Tipo: Text
Palavras-chave: Mass mortality; Bacterial; Vibrio; Hatcheries; Penaeus monodon; Shrimp.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/1993/acte-6100.PDF

Infection dynamics of a V. splendidus strain pathogenic to Mytilus edulis : In vivo and in vitro interactions with hemocytes
Provedor de dados: 5
Autores: Ben Cheikh, Yosra; Travers, Marie-agnes; Le Foll, Frank.
The pathogenic strain V. splendidus 10/068 1T1 has previously been reported for its virulence to the blue mussel and for its capacity to alter immune responses. In this study, we expanded the knowledge on hemocyte-pathogen interactions by using in vitro and in vivo assays. V. splendidus 10/068 1T1 severely inhibited cell adhesion and acidic vacuole formation unlike the innocuous phylogenetically related V. splendidus 12/056 M24T1 which had no effect on these cell functions. Furthermore, the virulent bacteria decreased hemocyte viability (59% of viability after 24 h). Infection dynamics were explored by using a model based on water tank cohabitation with septic mussels infected by GFP-tagged V. splendidus 10/068 1T1. Experimental infections were...
Tipo: Text
Palavras-chave: Innate immunity; Bivalves; Vibrio; Infection; Experimental model; Hemocyte.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00400/51136/53772.pdf

Isolation and characterization of a pathogenic bacterium specific to Manila clam Tapes philippinarum larvae
Provedor de dados: 5
Autores: Nicolas, Jean-louis; Ansquer, Dominique; Cochard, Jean-claude.
A bacterial strain belonging to the genus Vibrio was involved in a recurrent outbreak of disease in Manila clam Tapes philippinarum larvae at a commercial hatchery, This vibrio, named VTP for convenience, was not related to the known pathogenic vibrios and possessed unusual characteristics, Only Manila clam larvae and postlarvae were affected by the disease, Oyster and scallop larvae were not affected by vrp, Survival of vrp was found to be of short duration in seawater but even a very small number of cells (1 cell per 5 ml) could initiate the disease, VTP did not grow on thiosulfate-citratebile-sucrose agar and was resistant to chloramphenicol. An epidemiological study utilizing bioassays revealed the presence of VTP in adult bivalves and in the...
Tipo: Text
Palavras-chave: Bacterial diseases; Clam culture; Larvae; Pathogenic bacteria; Tapes philippinarum; Vibrio.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00037/14872/12198.pdf

Resistance to Antimicrobial Peptides in Vibrios
Provedor de dados: 5
Autores: Destoumieux-garzón, Delphine; Duperthuy, Marylise; Vanhove, Audrey; Schmitt, Paulina; Wai, Sun.
Vibrios are associated with a broad diversity of hosts that produce antimicrobial peptides (AMPs) as part of their defense against microbial infections. In particular, vibrios colonize epithelia, which function as protective barriers and express AMPs as a first line of chemical defense against pathogens. Recent studies have shown they can also colonize phagocytes, key components of the animal immune system. Phagocytes infiltrate infected tissues and use AMPs to kill the phagocytosed microorganisms intracellularly, or deliver their antimicrobial content extracellularly to circumvent tissue infection. We review here the mechanisms by which vibrios have evolved the capacity to evade or resist the potent antimicrobial defenses of the immune cells or tissues...
Tipo: Text
Palavras-chave: Vibrio; Lipopolysaccharide; Outer membrane vesicle; Membrane transporter; Innate immunity; Defensin; Cathelicidin; Bactericidal/permeability-increasing protein.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00276/38767/37308.pdf

Occurrence of Bacterial Pathogens and Human Noroviruses in Shellfish-Harvesting Areas and Their Catchments in France
Provedor de dados: 5
Autores: Rince, Alain; Baliere, Charlotte; Hervio-heath, Dominique; Cozien, Joelle; Lozach, Solen; Parnaudeau, Sylvain; Le Guyader, Francoise S.; Le Hello, Simon; Giard, Jean-christophe; Sauvageot, Nicolas; Benachour, Abdellah; Strubbia, Sofia; Gourmelon, Michele.
During a 2-year study, the presence of human pathogenic bacteria and noroviruses was investigated in shellfish, seawater and/or surface sediments collected from three French coastal shellfish-harvesting areas as well as in freshwaters from the corresponding upstream catchments. Bacteria isolated from these samples were further analyzed. Escherichia coli isolates classified into the phylogenetic groups B2, or D and enterococci from Enterococcus faecalis and E. faecium species were tested for the presence of virulence genes and for antimicrobial susceptibility. Salmonella members were serotyped and the most abundant serovars (Typhimurium and its monophasic variants and Mbandaka) were genetically characterized by high discriminative subtyping methods....
Tipo: Text
Palavras-chave: Campylobacter; Salmonella; Vibrio; HuNoVs; Fecal bacterial indicators; Shellfish; Water.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00461/57243/59243.pdf

Bacterial problems associated with scallop Pecten maximus larval culture
Provedor de dados: 5
Autores: Nicolas, Jean-louis; Corre, S; Gauthier, Gilles; Robert, Rene; Ansquer, Dominique.
Scallop Pecten maximus larvae have been cultured at the Argenton and Tinduff (Brittany, France) hatcheries with antibiotic treatment (chloramphenicol at 8 ppm) for 15 yr. Without treatment, outbreak of disease has normally occurred between Day 12 and Day 19 or sometimes earlier. A bacteriological study of larvae reared with and without antibiotic was performed over a 4 yr period. Among the collected strains, 2 clusters (C and F) of vibrios were present at high densities only in larvae cultured without treatment. One cluster (C) was routinely isolated over the 4 yr of study, while the other (F) was collected only in the third year. Their virulence with respect to scallop larvae and their lack of infectivity with respect to oyster larvae were demonstrated in...
Tipo: Text
Palavras-chave: Larvae; Bivalve; Vibrio; Scallop.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00090/20077/17716.pdf

Quantification of Vibrio penaeicida, the etiological agent of Syndrome 93 in New Caledonian shrimp, by real-time PCR using SYBR Green I chemistry
Provedor de dados: 5
Autores: Goarant, Cyrille; Merien, F.
Shrimp farming is a small but growing industry in New Caledonia. Since 1993, "Syndrome 93" has been affecting New Caledonian shrimp farming industry every cold season, causing severe epizootic mortalities in grow-out ponds and significant losses. Highly pathogenic strains of Vibrio penaeicida are considered the etiological agent of the disease in Litopenaeus stylirostris. On one hand, studies demonstrated that healthy shrimp may carry V penaeicida for weeks with a high overall prevalence, regardless of any seasonal pattern or temperature conditions. On the other hand, larvae are free of V penaeicida and are also resistant to experimental infection. V penaeicida is frequently detected in incoming water pumped from the bays, which was shown, by a molecular...
Tipo: Text
Palavras-chave: Vibriosis; Vibrio; Real time PCR; Quantification; Mariculture; Extraction techniques.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1903.pdf

Évolution de la virulence de V. crassostreae en lien avec l’huître en tant qu’hôte et les phages en tant que prédateurs
Provedor de dados: 5
Autores: Piel, Damien.
Understanding the ecological and evolutionary dynamics of infectious agents is important for diagnosing, predicting and preventing diseases in farmed and wild species. This project was aimed at studying the evolution of V. crassostreae virulence in relation to the oyster as a host and to the phages as predators. We identified the molecular mechanisms leading to the adaptation of vibrios to oysters. We demonstrated the emergence of virulent strains of V. crassostreae in the area impacted by the juvenile oyster mortality syndrome. The emergence of more virulent V. crassostreae strains is linked to the acquisition of a plasmid encoding a type 6 secretion system responsible for lethal activity towards oyster hemocytes. This project also provides knowledge on...
Tipo: Text
Palavras-chave: Vibrio; Interactions hôte/parasite; Emergence de pathogènes; Bactériophages; V. crassostreae; Mécanismes moléculaires; Vibrio; Host/parasite interactions; Pathogen emergence; Bacteriophages.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00690/80203/83289.pdf

Disturbance induced decoupling between host genetics and composition of the associated microbiome
Provedor de dados: 5
Autores: Wegner, Karl Mathias; Volkenborn, Nils; Peter, Hannes; Eiler, Alexander.
Background  Studies of oyster microbiomes have revealed that a limited number of microbes, including pathogens, can dominate microbial communities in host tissues such as gills and gut. Much of the bacterial diversity however remains underexplored and unexplained, although environmental conditions and host genetics have been implicated. We used 454 next generation 16S rRNA amplicon sequencing of individually tagged PCR reactions to explore the diversity of bacterial communities in gill tissue of the invasive Pacific oyster Crassostrea gigas stemming from genetically differentiated beds under ambient outdoor conditions and after a multifaceted disturbance treatment imposing stress on the host. Results  While the gill associated microbial communities in...
Tipo: Text
Palavras-chave: Microbiota; Population structure; Stress; Pathogen; Biological invasion; Pacific oyster; Crassostrea gigas; Vibrio; Mycoplasma.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00171/28202/26430.pdf

First molecular evidence of cross-species induction of metalloprotease gene expression in Vibrio strains pathogenic for Pacific oyster Crassostrea gigas involving a quorum sensing system
Provedor de dados: 5
Autores: De Decker, Sophie; Reynaud, Yann; Saulnier, Denis.
The aim of that studywas to explore the hypothesis that a quorumsensingmechanismmodulatesmetalloprotease gene expression of two Vibrio species pathogenic for oyster, at intraspecific (Vibrio splendidus) and interspecific (V. splendidus affecting V. aestuarianus) levels. Metalloprotease activities (Vsm for V. splendidus LGP32 and Vam for V. aestuarianus 02/041) and growth curves obtained by real-time PCR assay revealed cell density-dependent metalloprotease inductions triggered between 12 h and 16 h of culture. A quorum sensing assay was then developed using a conditioned medium prepared from supernatant of a mutant strain of V. splendidus LGP32 unable to produce metalloprotease. Specific real-time qPCR assays targeting metalloprotease genes vsm and vam...
Tipo: Text
Palavras-chave: Quorum sensing; Crassostrea gigas; Vibrio; Metalloprotease; Gene expression.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00124/23539/21381.pdf

Effect of the probiotic strain Phaeobacter gallaeciensis after bacterial challenge on the complete larval development of Pecten maximus
Provedor de dados: 5
Autores: Genard, Bertrand; Larouche, Olivier; Nicolas, Jean-louis; Miner, Philippe; Beaudin, Marie-lou; Tremblay, Rejean.
The aim of this project was to evaluate the impact of probiotic (Phaeobacter gallaeciensis, X34 strain) treatment on the complete development (from veliger to metamorphosis) of Pecten maximus larvae in the context of a bacterial challenge and in conditions more representative of hatchery practices. To that effect, the present study was divided into two main steps. In the first, we used in vitro analyses (antibiograms and microplate assays) to validate the inhibition abilities of X34 on the growth of four Vibrio pathogen species. During the second step, we added pathogens (Vibrio pectenicida) into rearing tanks after two weeks of pre-treatment with the probiotic and then followed the larval development of Pecten maximus through the monitoring of survival...
Tipo: Text
Palavras-chave: Probiotics; Hatchery; Bacterial challenge; Larval development; Pecten maximus; Phaeobacter gallaeciensis; Vibrio.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00213/32429/30895.pdf

Effect of in vivo pesticide exposure and injection of bacteria on immune gene expression in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Renault, Tristan; Thomas Guyon, Hélène; Burgeot, Thierry.
Bay of Marennes-Oleron is an important French area of reproduction for sea bass, Dicentrarchus labrax. This area is often subjected to many pollutions due to anthropic activities, including pesticides and polycyclic aromatic hydrocarbons (PAH) which cause adverse effects in aquatic organisms. The harmful effects of such   contaminants on fish immune system leading to a decreased resistance against pathogens begin to be described, but remain poorly  known. In this context, influence of micropolluants, like PAH, on the defense mechanisms developed by sea bass was tested. Fish were exposed five days to a soluble fraction of PAH with environmental concentration (760 ng/L). At the end of the contamination, fish were transferred to non contaminated sea water and...
Tipo: Text
Palavras-chave: Phagocytosis; Vibrio; Pesticides; Pollutant; Crasssotrea gigas; Oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3317.pdf

Toxic factors of Vibrio strains pathogenic to shrimp
Provedor de dados: 5
Autores: Goarant, Cyrille; Herlin, Jose; Brizard, Raphael; Marteau, Anne-laure; Martin, C; Martin, B.
Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida...
Tipo: Text
Palavras-chave: Vibrio; Vibrio penaeicida; Shrimp; Extracellular products; Temperature.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00000/10543/7600.pdf

Etude ecologique des vibrions du bassin d'Arcachon
Provedor de dados: 5
Autores: Marchand, Michel.
Water and sediment samples monthly collected from Arcachon Bay (Gironde, France) were examined during three years for the presence of marine vibrios. V. cholerae serotype 01 was never detected. On the other hand, the Eyre River and mouth contain non-agglutinating V. cholerae (127 strains) principally in water. The low temperatures slow their development down without stopping it completely. V. parahaemolyticus (257 strains) present in the same areas is particularly isolated from water when the aquatic medium temperature rises to 15 degree C. V. alginolyticus (638 strains) settles the whole Bay, except the river, in any season. The low temperatures promote growth of V. metschnikovii (203 strains) very tolerant towards salinity. V. anguillarum (46 strains)...
Tipo: Text
Palavras-chave: Vibrio metschnikovii; Vibrio anguillarum; Vibrio alginolyticus; Vibrio cholerae; Vibrio; Marine environment; Microorganisms; Sediments; Salinity effects; Temperature effects; Ecology; Water.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-998.pdf

Highly diverse recombining populations of Vibrio cholerae and Vibrio parahaemolyticus in French Mediterranean coastal lagoons
Provedor de dados: 5
Autores: Esteves, Kevin; Mosser, Thomas; Aujoulat, Fabien; Hervio-heath, Dominique; Monfort, Patrick; Jumas-bilak, Estelle.
Vibrio parahaemolyticus and Vibrio cholerae are ubiquitous to estuarine and marine environments. Thes two species found in Mediterranean coastal systems can induce infection in humans. Environmental isolates of V. cholerae (n = 109) and V. parahaemotycius (n = 89) sampled at different dates, stations and water salinities were investigated for virulence genes and by a mutilocus sequence-based analysis (MLSA). V. cholerae isolate were all ctxA negative and only one isolate of V. parahaemolyticus displayed trh2 gene. Most Sequence Types (ST) corresponded to uique ST isolated at one date or one station. Frequent recombination events were detected among different pathogenic species, V. parahaemotyticus, V. cholerae, Vibrio mimicus, and Vibrio metoecus....
Tipo: Text
Palavras-chave: Vibrio; Human pathogens; French coastal lagoons; Phylogeny; Recombination; Multi-locus sequence analysis; Virulence factor.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00274/38528/37039.pdf

First evidence of a potential antibacterial activity involving a laccase-type enzyme of the phenoloxidase system in Pacific oyster Crassostrea gigas haemocytes
Provedor de dados: 5
Autores: Luna-acosta, Andrea; Saulnier, Denis; Pommier, Mylene; Haffner, Philippe; De Decker, Sophie; Renault, Tristan; Thomas-guyon, Helene.
Phenoloxidases (POs) are a group of copper proteins including tyrosinase, catecholase and laccase. In several insects and crustaceans, antibacterial substances are produced through the PO cascade, participating in the direct killing of invading microorganisms. However, although POs are widely recognised as an integral part of the invertebrate immune defence system, experimental evidence is lacking that these properties are conserved in molluscs, and more particularly in the Pacific oyster Crassostrea gigas. In the present study, Vibrio splendidus LGP32 and Vibrio aestuarianus 02/041 growths were affected, after being treated with C. gigas haemocyte lysate supernatant (HLS), and either a common substrate of POs, l-3,4-dihydroxyphenylalanine (l-DOPA), to...
Tipo: Text
Palavras-chave: Phenoloxidases; Antibacterial activity; Vibrio; Bivalve; Immunity.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00045/15598/13001.pdf

First description of French V. tubiashii strains pathogenic to mollusk: II. Characterization of properties of the proteolytic fraction of extracellular products
Provedor de dados: 5
Autores: Mersni Achour, Rachida; Imbert-auvray, Nathalie; Huet, Valerie; Ben Cheick, Yosra; Faury, Nicole; Doghri, Ibtissem; Rouatbi, Sonia; Bordenave, Stéphanie; Travers, Marie-agnes; Saulnier, Denis; Fruitier-arnaudin, Ingrid.
Extracellular products (ECPs) of the French V. tubiashii strain 07/118 T2 were previously reported to be toxic for the Pacific oyster Crassostrea gigas. In this study we now assessed host cellular immune responses and bacterial potential effectors by which these ECPs can be associated with host damages. The adhesion capacity (28% inhibition) and phagocytosis ability (56% inhibition) of oyster hemocytes were the main functions affected following in vitro contact between hemocytes and V. tubiashii ECPs. This may be linked to the demonstration of the capability of ECPs to cleave various cellular substrates as oyster collagen. Moreover, a strong metalloproteolytic activity was recorded with general (azocasein) and specific (ADAM) substrates and characterized...
Tipo: Text
Palavras-chave: ECPs; Oyster immune responses; Thermolysin-metalloprotease; Mass spectroscopy; ADAM substrate; Vibrio.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00211/32203/30642.pdf

Effect of ionizing radiation onVibrio bacteria in Crassostrea virginica (American oyster)
Provedor de dados: 5
Autores: Eberiel, D; Dooley, K; Langton, L; Malleth, J.
The presence of Vibrio bacteria in raw shellfish poses a significant health hazard to the consuming public. These human pathogens are natural, seasonal components of the microbial flora of shellfish which can result in primary septicemic or diarrheal fatal infections. Existing shellfish depuration neither targets nor eliminates the Vibrio bacteria. For this reason, additional purification processes are necessary. In this study, the effect of low dose gamma radiation on the inactivation ofVibrio cholerae (01 and non 01) and vulnificus is investigated. Log decrement (D10) values ranging only to a high of 0.09 KGy in the Vibrio strains support the hypothesis that food irradiation technology can provide an additional effective and efficient shellfish...
Tipo: Text
Palavras-chave: USA Coasts; USA; Vibrio vulnificus; Vibrio cholerae; Crassostrea virginica; Bivalvia; Vibrio; Bacteria; Self purification; Marine molluscs; Irradiation; Gamma radiation; Ionizing radiation; Oyster culture.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1619.pdf

Uso de probióticos na produção de pós-larvas de camarão-rosa
Provedor de dados: 86
Autores: Silva,Emanuell Felipe Beserra da; Fróes,Charles Nunes; Souza,Diego Moreira de; Soares,Roberta; Peixoto,Silvio; Wasielesky,Wilson; Ballester,Eduardo Luis Cupertino.
O objetivo deste trabalho foi avaliar o uso de probióticos na produção de pós-larvas do camarão-rosa (Farfantepenaeus brasiliensis). Utilizou-se o delineamento experimental inteiramente casualizado, com dois tratamentos - uso de probióticos (Bacillus spp.) e controle com uso de antibiótico (eritromicina) - e quatro repetições. Pós-larvas no estágio PL1 foram estocadas em cada parcela experimental (3,0 L) e cultivadas até o estágio PL10. As variáveis de qualidade de água e o desenvolvimento de bactérias do gênero Vibrio, na água de cultivo, foram monitorados ao longo do experimento. Ao final do período experimental, as pós-larvas foram avaliadas qualitativamente e quanto à sobrevivência, ao peso e ao comprimento. Nas variáveis de qualidade de água, apenas o...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus; Farfantepenaeus brasiliensis; Vibrio; Antibiótico; Crescimento; Sobrevivência.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000600019

Lactic acid bacteria used as preservative in fresh feed for marine shrimp maturation
Provedor de dados: 86
Autores: Vieira,Genyess Ribeiro Arthur da Silva; Soares,Mariana; Ramírez,Norha Constanza Bolívar; Schleder,Delano Dias; Silva,Bruno Corrêa da; Mouriño,José Luiz Pedreira; Andreatta,Edemar Roberto; Vieira,Felipe do Nascimento.
Abstract The objective of this work was to evaluate the effect of Lactobacillus plantarum on the preservation of fresh mussels and its effect on the attractiveness, consumption, and midgut bacterial microbiota of Pacific white shrimp broodstock. The experiment evaluated mussels stored with L. plantarum at 4ºC. The controls were: mussels stored at -18ºC without L. plantarum; mussels stored at -18ºC with L. plantarum; and mussels stored at 4ºC without L. plantarum. Microbiological analyses on mussels were performed on days 1, 7, 15, 30, 45, and 60 after processing. Additionally, mussels preserved with L. plantarum and stored at 4ºC, and mussels stored at -18ºC without L. plantarum were evaluated after 15 days for attractiveness, consumption, and midgut...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus plantarum; Litopenaeus vannamei; Vibrio; Probiotic.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016001101799

Experimental infection with different bacterial strains in larvae and juvenile Litopenaeus vannamei reared in Santa Catarina State, Brazil - doi: 10.4025/actascibiolsci.v32i3.5471
Provedor de dados: 3
Autores: Buglione, Celso Carlos; UFSC; Vieira, Felipe do Nascimento; UFSC; Mouriño, José Luiz Pedreira; UFSC; Pedrotti, Fabiola Santiago; UFSC; Jatoba, Adolfo; UFSC; Martins, Maurício Laterça; UFSC.
This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their  virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7%...
Tipo: Resistencia a infecção
Palavras-chave: 2.12.01.02-1 Shrimp; Experimental infection; Vibrio; Virulence; Immunology bacteriologia Shrimp; Experimental infection; Vibrio; Virulence; Immunology.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5471

Adherence assays and Slime production of Vibrio alginolyticus and Vibrio parahaemolyticus
Provedor de dados: 58
Autores: Abdallah,Fethi Ben; Chaieb,Kamel; Zmantar,Tarek; Kallel,Hela; Bakhrouf,Amina.
In this study we investigated the phenotypic slime production of Vibrio alginolyticus and Vibrio parahaemolyticus strains, food-borne pathogens, using a Congo red agar plate assay. Furthermore, we studied their ability to adhere to abiotic surfaces and Vero cells line. Our results showed that only V. alginolyticus ATCC 17749 was a slime-producer developing almost black colonies on Congo red agar plate. Adherence to glace tube showed that all V. alginolyticus strains were more adherent than V. parahaemolyticus. Only V. alginolyticus ATCC 17749 was found to be able to form biofilm on polystyrene microplate wells (OD570 = 0.532). Adherence to Vero cells showed that all tested strains were non adherent after 30 min, however after 60 min all the studied strains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vibrio; Slime production; Adherence; Abiotic surface; Vero cells.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200033