Consulta: subjectFacets:"Viral DNA"
Registros recuperados: 2
Data/hora: 13/06/2024 21:38:45

Detection of ostreid herpesvirus 1 (OsHV-1) DNA in seawater by PCR: influence of water parameters in bioassays
Provedor de dados: 5
Autores: Vigneron, Vassilia; Solliec, Gaelle; Montanie, Hélène; Renault, Tristan.
Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted...
Tipo: Text
Palavras-chave: Temperature; PCR; Detection; Seawater; Viral DNA; Oyster herpesvirus 1.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2909.pdf

Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat
Provedor de dados: 5
Autores: Segarra, Amelie; Baillon, Laury; Faury, Nicole; Tourbiez, Delphine; Renault, Tristan.
High mortality rates are reported in spat and larvae of Pacific oyster Crassostrea gigas and associated with ostreid herpesvirus 1 (OsHV-1) detection in France. Although the viral infection has been experimentally reproduced in oyster larvae and spat, little knowledge is currently available concerning the viral entry and its distribution in organs and tissues. This study compares OsHV-1 DNA and RNA detection and localization in experimentally infected oysters using two virus doses: a low dose that did not induce any mortality and a high dose inducing high mortality. Real time PCR demonstrated significant differences in terms of viral DNA amounts between the two virus doses. RNA transcripts were detected in oysters receiving the highest dose of viral...
Tipo: Text
Palavras-chave: Crassostrea gigas; In situ hybridization; Ostreid herpesvirus 1; Viral DNA; Viral RNA.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00300/41099/40272.pdf