Consulta: subjectFacets:"Vitellogenin"
Registros recuperados: 3
Data/hora: 10/06/2024 03:44:53

Protein content and electrophoretic profile of fat body and ovary extracts from workers of Melipona quadrifasciata anthidioides (Hymenoptera, Meliponini)
Provedor de dados: 76
Autores: Oliveira,Vagner T. Paes de; Cruz-Landim,Carminda da.
Workers of Melipona quadrifasciata anthidioides (Lepeletier, 1836) develop their ovaries and lay eggs, therefore the production of vitellogenin is expected. In electrophoretic profiles only fat body extracts from nurse workers and ovary extracts from newly-emerged workers show protein with molecular mass similar to vitellogenin. However, an increase in the protein content was detected in forager fat body. This increase was attributed to storage of vitellogenin or other proteins in the previous phase and not discharged into the hemolymph or to an effect of the increased titre of juvenile hormone in this phase of worker life over the fat body functioning.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stingless bees; Fat body; Ovary; Vitellogenin; Oogenesis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212004000400010

Homologous ELISA procedure for the determination of penaeid shrimp vitellogenin
Provedor de dados: 5
Autores: Mendoza, Roberto; Guillaume, Jean-charles; Fauvel, Christian.
Vitellogenin (VTG) was isolated from the haemolymph of Penaeus vannamei by a three step procedure including ultracentrifugation, gel filtration and ion exchange chromatography. VTG was used to raise polyclonal antibodies that were purified by ion exchange chromatography. A two step competitive assay was developed in which VTG could be quantitated by its capacity to inhibit the binding of antibody to the VTG previously adsorbed onto a solid phase. Sensitivity from equilibrium and from non-equilibrium assays was 41 and 2.3 ng/ml respectively. Estimates of within-assay and between-assay variabilities of standard curves were 4.2 and 9.6% respectively. The inhibition curves for dilutions of haemolymph from vitellogenic females, egg yolk extracts and purified...
Tipo: Text
Palavras-chave: Pénéidé; Vitellogénine; Vitelline; Immuno-essai (ELISA); Anticorps; Penaeid; Vitellogenin; Vitellin; Immunoassay (ELISA); Antibody.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00188/29894/28333.pdf

Effect of genistein-enriched diets on the endocrine process of gametogenesis and on reproduction efficiency of the rainbow trout Oncorhynchus mykiss
Provedor de dados: 5
Autores: Bennetau Pelissero, Catherine; Breton, Bernard; Bennetau, Bernard; Corraze, Genevieve; Le Menn, Françoise; Davail Cuisset, Blandine; Helou, Chantal; Kaushik, Sadasivam.
Three practical diets were formulated to contain 0, 500, or 1000 ppm genistein. The three diets were distributed for 1 year to groups of rainbow trout undergoing their first gametogenesis and until spawning. Growth performance of rainbow trout was not affected by dietary treatments. Plasma cholesterol levels were equivalent between groups. In males, a slight but constant induction of vitellogenin (VTG) synthesis and a decrease in testosterone levels were observed. A slight decrease in plasma levels of beta FSH and beta LH was noticed at the end of spermatogenesis in the male fish fed a diet with 500 ppm (genistein) (from 2.16 +/- 0.39 to 1.47 +/- 0.23 for beta FSH and from 0.44 +/- 0.09 to 0.31 +/- 0.09 for beta LH). There was a significantly reduced 17...
Tipo: Text
Palavras-chave: Diet; Reproduction; GTH; Vitellogenin; Steroid; Trout; Genistein.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-738.pdf