Consulta: subjectFacets:"WSSV"
Registros recuperados: 9
Data/hora: 01/06/2024 01:10:46

Comparative transcriptome and potential antiviral signaling pathways analysis of the gills in the red swamp crayfish, Procambarus clarkii infected with White Spot Syndrome Virus (WSSV)
Provedor de dados: Genetics and Molecular Biology
Autores: Du,Zhi-Qiang; Jin,Yan-Hui.
Abstract Red swamp crayfish is an important model organism for research of the invertebrate innate immunity mechanism. Its excellent disease resistance against bacteria, fungi, and viruses is well-known. However, the antiviral mechanisms of crayfish remain unclear. In this study, we obtained high-quality sequence reads from normal and white spot syndrome virus (WSSV)-challenged crayfish gills. For group normal (GN), 39,390,280 high-quality clean reads were randomly assembled to produce 172,591 contigs; whereas, 34,011,488 high-quality clean reads were randomly assembled to produce 182,176 contigs for group WSSV-challenged (GW). After GO annotations analysis, a total of 35,539 (90.01%), 14,931 (37.82%), 28,221 (71.48%), 25,290 (64.05%), 15,595 (39.50%), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Procambarus clarkii; WSSV; Gills; Illumina sequencing; De novo assembly; Comparative transcriptomics.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100168

Transmission per os du white spot syndrome virus : facteur limitant l'extension de la maladie chez les crustacés marins
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Hernandez Herrera, Rosa Idalia.
Penaeid family shrimp constitute the first aquaculture product in the world in terms of commercial value. They are produced in third world countries of the sub-equatorial belt. Among the causes limiting their production, one is the presence of the WSSV (White Spot Syndrome Virus), a pathogenic agent that produces massive mortality. Our aim was to investigate the first stages of the viral infection, in order to be used as target of prophylactic actions. A fish cell line (SSN-1) was used as model to tentatively develop in vitro studies. Only defective particles were produced confirming the high specificity to crustacea of the infection with crustacean virus. Electron microscopy showed structural similarities between the WSSV and B, B2 and Baculo-B viruses of...
Tipo: Text
Palavras-chave: Electron Microscope; Immunodetection; Dot blot; ELISA  sandwich; Cell line SSN1; MrNv; B2 virus; WSSV; Microscopie électronique à transmission; Immunodetection; Dot blot; Sandwich ELISA; Lignée cellulaire SSN1; MrNv; Virus B2; WSSV.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/these-6650.pdf

NMR structure of mussel mytilin, and antiviral-antibacterial activities of derived synthetic peptides
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Roch, Philippe; Yang, Y; Toubiana, Mylene; Aumelas, A.
Mytilin is a 34-residue antibacterial peptide from the mussel Mytilus galloprovincialis, which in addition possesses in vitro antiviral activity. The three-dimensional solution structure of the synthetic mytilin was established by using 1H NMR and consists of the common cysteine-stabilized alpha beta motif close to the one observed in the mussel defensin MGD-1. Mytilin is characterized by 8 cysteines engaged in four disulfide bonds (2-27, 6-29, 10-31, and 15-34) only involving the beta-strand II. Hydrophilic and hydrophobic areas of mytilin account for 63% and 37%, respectively, a ratio very close to that of MGD-1 (64% and 36%). One linear and three cyclic fragments were designed from the interstrand loop sequence known to retain the biological activities...
Tipo: Text
Palavras-chave: Shrimp; Molluscs; Mussel; Antibacterial activity; WSSV; Viral protection; Beta hairpin structure; NMR; Mytilin; Antimicrobial peptide.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4485.pdf

Parâmetros de cultivo e a enfermidade da mancha-branca em fazendas de camarões de Santa Catarina
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Costa,Sérgio Winckler da; Vicente,Luiz Rodrigo Mota; Souza,Talita Medeiros de; Andreatta,Edemar Roberto; Marques,Maria Risoleta Freire.
O objetivo deste trabalho foi avaliar a relação entre parâmetros físico-químicos e biológicos do cultivo de camarão com a enfermidade causada pelo vírus da síndrome da mancha-branca (WSSV). Foram avaliadas a mortalidade, as características físicas e químicas da água e do solo, e foram realizados os exames e as análises microscópicas a fresco, presença de víbrios na hemolinfa, além do diagnóstico histopatológico e molecular (PCR) do WSSV, em viveiros de oito fazendas de Santa Catarina, entre 2008 e 2009. O vírus foi detectado em cinco fazendas com registros da enfermidade em ciclos anteriores. A temperatura da água foi semelhante entre as fazendas com e sem WSSV, e a mortalidade ocorreu a intervalos ascendentes entre 24,6 e 29,3ºC. Não foram encontradas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Litopenaeus vannamei; Alcalinidade; Fenol; Vírus da mancha-branca; WSSV.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001200025

Presença do vírus da síndrome da mancha branca em crustáceos decápodes silvestres em lagoas costeiras no Sul do Brasil
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Costa,S.W.; Fraga,A.P.M.; Zamparetti,A.S.; Marques,M.R.F.; Andreatta,E.R.
A presença do vírus da síndrome da mancha branca (em inglês WSSV) nas principais espécies de camarões, siris e caranguejos de cinco lagoas que recebem o efluente de fazendas afetadas pela enfermidade foi detectada por nested PCR, e inclusões virais nos camarões por histologia. Pela nested PCR encontrou-se a presença de WSSV em 13 de 16 (81,2%) amostras de camarões da espécie Farfantepenaeus paulensis, em 13 de 14 (92,8%) de Litopenaeus schmitti, em uma de duas de Farfantepenaeus brasiliensis (50%), em 13 de 15 (86,6%) de siri da espécie Callinectes danae e em 11 de 12 (91,6%) de Callinectes sapidus, e não foi detectada no caranguejo Chasmagnathus granulata em 10 amostras. Inclusões características de WSSV foram observadas em três amostras histológicas de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: WSSV; Vírus; Camarão; Siri; Caranguejo.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000100030

Hypoxia increases susceptibility of Pacific white shrimp to whitespot syndrome virus (WSSV)
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Lehmann,M.; Schleder,D.D.; Guertler,C.; Perazzolo,L.M.; Vinatea,L.
The present study aimed to evaluate the mortality, reactive oxygen species production (ROS) and total hemocyte counts (THC) of the marine shrimp Litopenaeus vannamei infected with the white spot syndrome virus (WSSV) at three levels of oxygen saturation. For this, 360 shrimp (20±2g) were distributed in 24 tanks (60L), divided in two groups (infected and non-infected), which were subjected to 30, 60 and 100% of dissolved oxygen saturation (in quadruplicate). During 96 hours after infection, daily hemolymph samples were collected for hemato-immunological parameter evaluation (THC and ROS) and dead animals were removed and computed to assess cumulative mortality rates. In the infected group, animals subjected to 100% saturation showed higher ROS production...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Litopenaeus vannamei; WSSV; Dissolved oxygen saturation; Immune response; Mortality.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200397

Molecular cloning and recombinant expression of the VP28 carboxyl-terminal hydrophilic region from a brazilian white spot syndrome virus isolate
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Braunig,Patricia; Rosa,Rafael Diego da; Seibert,Caroline Heidrich; Borsa,Mariana; Stoco,Patricia Hermes; Grisard,Edmundo Carlos; Pinto,Aguinaldo Roberto.
In the present study, a fragment of the VP28 coding sequence from a Brazilian WSSV isolate (BrVP28) was cloned, sequenced and expressed in E. coli BL21(DE3) pLysS strain in order to produce the VP28 carboxyl-terminal hydrophilic region. The expression resulted in a protein of about 21 kDa, which was purified under denaturing conditions, resulting in a final highly purified BrVP28 preparation. The recombinant protein obtained can be used in several biotechnology applications, such as the production of monoclonal antibodies which could be used in the development of diagnostic tools as well as in the studies on the characterization of white spot syndrome virus (WSSV) isolated in Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: WSSV; VP28; Recombinant protein; Penaeid shrimp.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200023

Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
Provedor de dados: Brazilian Journal of Microbiology
Autores: Leal,C.A.G.; Carvalho-Castro,G.A.; Cottorello,A.C.; Leite,R.C.; Figueiredo,H.C.P.
The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clinical samples; Sensitivity; Real-time PCR; WSSV; WSD.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300038

First report of White spot syndrome virus in farmed and wild penaeid shrimp from Lagoa dos Patos estuary, southern Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Cavalli,Lissandra Souto; Romano,Luis Alberto; Marins,Luis Fernando; Abreu,Paulo César.
In this study, we detected White spot syndrome virus (WSSV) in wild Farfantepenaeus paulensis collected in the Lagoa dos Patos estuary and cultivated Litopenaeus vannamei. This is the first report of WSSV in F. paulensis from Lagoa dos Patos and farmed L. vannamei shrimps in Rio Grande do Sul.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: WSSV; Viral diseases; Farfantepenaeus paulensis; Litopenaeus vannamei; Epizootiology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300042