Consulta: subjectFacets:"White spot syndrome virus"
Registros recuperados: 2
Data/hora: 09/06/2024 09:43:21

Exploring the Impact of the Biofloc Rearing System and an Oral WSSV Challenge on the Intestinal Bacteriome of Litopenaeus vannamei
Provedor de dados: 5
Autores: Pilotto, Mariana R.; Goncalves, Andre N. A.; Vieira, Felipe N.; Seifert, Walter Q.; Bachere, Evelyne; Rosa, Rafael D.; Perazzolo, Luciane M.
We provide a global overview of the intestinal bacteriome of Litopenaeus vannamei in two rearing systems and after an oral challenge by the White spot syndrome virus (WSSV). By using a high-throughput 16S rRNA gene sequencing technology, we identified and compared the composition and abundance of bacterial communities from the midgut of shrimp reared in the super-intensive biofloc technology (BFT) and clear seawater system (CWS). The predominant bacterial group belonged to the phylum Proteobacteria, followed by the phyla Bacteroidetes, Actinobacteria, and Firmicutes. Within Proteobacteria, the family Vibrionaceae, which includes opportunistic shrimp pathogens, was more abundant in CWS than in BFT-reared shrimp. Whereas the families Rhodobacteraceae and...
Tipo: Text
Palavras-chave: Biofloc technology (BFT); Penaeid shrimp; Gut microbiota; White spot syndrome virus; 16S rRNA gene sequencing.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00454/56546/58256.pdf

Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry Techniques
Provedor de dados: 77
Autores: Hipolito,M; Catroxo,M. H. B; Martins,A. M. C. R. P. F; Melo,N.A; Pituco,E. M; Galleti,N.T.C; Ranzani-Paiva,M. J. T; Mouriño,J.L.P; Ferreira,C.M.
In this study thirty shrimp samples from commercial marine shrimp (L. vannamei) farms of southern region of Brazil were obtained. Hepatopancreas and shell scrapings fragments collected in these animals were processed by transmission electron microscopy using negative staining (rapid preparation), immunoelectron microscopy and immunocytochemistry (immunolabelling with colloidal gold particles) techniques. On the transmission electron microscopy a great number of white spot virus particles, ovoid or bacilliform-to-ellipsoid, measured 230-290 nm in length and 80-160 nm in diameter with intra-nuclear projections were visualized by the negative staining technique in 27 (90%) out of 30 samples examined. Using immunoelectron microscopy technique, the anti-VP 664...
Tipo: Journal article
Palavras-chave: White spot syndrome virus; Negative staining; Immunoelectron microscopy; Immunocytochemistry; Marine shrimp; Litopenaeus vannamei.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200067