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A model for floral color inheritance in Leucaena (Leguminosae)
Provedor de dados: Genetics and Molecular Biology
Autores: Simioni,Carine; Schifino-Wittmann,Maria Teresa; Paim,Nilton Rodrigues.
A population of 513 hybrids of Leucaena leucocephala (4x) and L. diversifolia ssp. diversifolia (4x), that is being analyzed for several morphological and agronomical characteristics, aiming at selecting cold tolerant plants, was also analyzed for floral color. A model of additive dominant inheritance (pink color dominant over white color, or presence versus absence of pigment, and the number of dominant alleles present determining the intensity of the pink color) is proposed. The action of two independent locus sets is also suggested, one for the male (with two main structural genes) and another for the female floral organs (one main structural gene). It is suggested that an undetermined number of regulatory genes affect color expression.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300013

Association between the g.296596G &gt; A genetic variant of RELN gene and susceptibility to autism in a Chinese Han population
Provedor de dados: Genetics and Molecular Biology
Autores: Fu,Xiaoyan; Mei,Zhu; Sun,Lixin.
Autism is a childhood neuro-developmental disorder, and Reelin (RELN) is an important candidate gene for influencing autism. This study aimed at investigating the influence of genetic variants of the RELN gene on autism susceptibility. In this study, 205 autism patients and 210 healthy controls were recruited and the genetic variants of the RELN gene were genotyped by the created restriction site-polymerase chain reaction (CRS-PCR) method. The influence of genetic variants on autism susceptibility was analyzed by association analysis, and the g.296596G &gt; A genetic variant in exon10 of the RELN gene was detected. The frequencies of allele/genotype in autistic patients were significantly different from those in healthy controls, and a statistically...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autism; Susceptibility; Association analysis; RELN gene; Genetic variants.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400003

Genetic diversity in natural populations of Jacaranda decurrens Cham. determined using RAPD and AFLP markers
Provedor de dados: Genetics and Molecular Biology
Autores: Bertoni,Bianca W.; Telles,Mariana P. de C.; Malosso,Milena G.; Torres,Simone C.Z.; Pereira,José O.; Lourenço,Mirian V.; França,Suzelei de C.; Pereira,Ana M.S.
Jacaranda decurrens (Bignoniaceae) is an endemic species of the Cerrado with validated antitumoral activity. The genetic diversity of six populations of J. decurrens located in the State of São Paulo was determined in this study by using molecular markers for randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). Following optimization of the amplification reaction, 10 selected primers generated 78 reproducible RAPD fragments that were mostly (69.2%) polymorphic. Two hundred and five reproducible AFLP fragments were generated by using four selected primer combinations; 46.3% of these fragments were polymorphic, indicating a considerable level of genetic diversity. Analysis of molecular variance (AMOVA) using these two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bignoniaceae; Carobinha; Cerrado; Germplasm bank; Preservation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300025

Distribution of a Ty3/gypsy-like retroelement on the A and B-chromosomes of Cestrum strigilatum Ruiz &amp;amp; Pav. and Cestrum intermedium Sendtn. (Solanaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Fregonezi,Jéferson Nunes; Vilas-Boas,Laurival A.; Fungaro,Maria Helena Pelegrinelli; Gaeta,Marcos Letaif; Vanzela,André Luís Laforga.
Retroelements are a diversified fraction of eukaryotic genomes, with the Ty1/copia and Ty3/gypsy groups being very common in a large number of plant genomes. We isolated an internal segment of the Ty3/gypsy retroelement of Cestrum strigilatum (Solanaceae) using PCR amplification with degenerate primers for a conserved region of reverse transcriptase. The isolated segment (pCs12) was sequenced and showed similarity with Ty3/gypsy retroelements of monocotyledons and dicotyledons. This segment was used as probe in chromosomes of C. strigilatum and Cestrum intermedium. Diffuse hybridization signals were observed along the chromosomes and more accentuated terminal signals in some chromosome pairs, always associated with nucleolus organizer regions (NORs). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cestrum strigilatum; Cestrum intermedium; Karyotype organization; Retroelements; Solanaceae; Ty3-gypsy.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400017

Natural triploidy in Leporinus cf. elongatus bearing sex chromosomes
Provedor de dados: Genetics and Molecular Biology
Autores: Molina,Wagner Franco; Margarido,Vladimir Pavan; Galetti Jr,Pedro Manoel.
Although several cases of natural triploidy in fish have already been described, spontaneous polyploidy in species with differentiated sex chromosomes are rare. We report the occurrence of a triploid fish (3n = 81) Leporinus cf. elongatus, a species characterized by a highly differentiated ZZ/ZW sex chromosome system, from the São Francisco river. The occurrence of a ZZZ triploid adult indicates the viability of this chromosome constitution in this fish.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Polyploidy; Triploid fish; ZW chromosomes; Sex chromosomes; Anostomidae.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400010

Relative frequency and estimated minimal frequency of Lysosomal Storage Diseases in Brazil: Report from a Reference Laboratory
Provedor de dados: Genetics and Molecular Biology
Autores: Giugliani,Roberto; Federhen,Andressa; Michelin-Tirelli,Kristiane; Riegel,Mariluce; Burin,Maira.
Abstract Lysosomal storage diseases (LSDs) comprise a heterogeneous group of more than 50 genetic conditions of inborn errors of metabolism (IEM) caused by a defect in lysosomal function. Although there are screening tests for some of these conditions, diagnosis usually depends on specific enzyme assays, which are only available in a few laboratories around the world. A pioneer facility for the diagnosis of IEM and LSDs was established in the South of Brazil in 1982 and has served as a reference service since then. Over the past 34 years, samples from 72,797 patients were referred for investigation of IEM, and 3,211 were confirmed as having an LSD (4.41%, or 1 in 22), with 3,099 of these patients originating from Brazil. The rate of diagnosis has increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lysosomal storage diseases; Epidemiology; Reference center; Biochemical genetics; Brazil.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100031

Genomic imbalances in esophageal squamous cell carcinoma identified by molecular cytogenetic techniques
Provedor de dados: Genetics and Molecular Biology
Autores: Bellini,Marilanda Ferreira; Silva,Ana Elizabete; Varella-Garcia,Marileila.
This review summarizes the chromosomal changes detected by molecular cytogenetic approaches in esophageal squamous cell carcinoma (ESCC), the ninth most common malignancy in the world. Whole genome analyses of ESCC cell lines and tumors indicated that the most frequent genomic gains occurred at 1, 2q, 3q, 5p, 6p, 7, 8q, 9q, 11q, 12p, 14q, 15q, 16, 17, 18p, 19q, 20q, 22q and X, with focal amplifications at 1q32, 2p16-22, 3q25-28, 5p13-15.3, 7p12-22, 7q21-22, 8q23-24.2, 9q34, 10q21, 11p11.2, 11q13, 13q32, 14q13-14, 14q21, 14q31-32, 15q22-26, 17p11.2, 18p11.2-11.3 and 20p11.2. Recurrent losses involved 3p, 4, 5q, 6q, 7q, 8p, 9, 10p, 12p, 13, 14p, 15p, 18, 19p, 20, 22, Xp and Y. Gains at 5p and 7q, and deletions at 4p, 9p, and 11q were significant prognostic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CGH; Esophageal carcinoma; FISH; Genomic imbalances; Molecular cytogenetics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200001

Improving the production of transgenic fish germlines: in vivo evaluation of mosaicism in zebrafish (Danio rerio) using a green fluorescent protein (GFP) and growth hormone cDNA transgene co-injection strategy
Provedor de dados: Genetics and Molecular Biology
Autores: Figueiredo,Márcio de Azevedo; Lanes,Carlos Frederico Ceccon; Almeida,Daniela Volcan; Marins,Luis Fernando.
In fish, microinjection is the method most frequently used for gene transfer. However, due to delayed transgene integration this technique almost invariably produces mosaic individuals and if the gene is not integrated into germ cells its transmission to descendants is difficult or impossible. We evaluated the degree of in vivo mosaicism using a strategy where a reporter transgene is co-injected with a transgene of interest so that potential germline founders can be easily identified. Transgenic zebrafish (Danio rerio) were produced using two transgenes, both comprised of the carp beta-actin promoter driving the expression of either the green fluorescent protein (GFP) reporter gene or the growth hormone cDNA from the marine silverside fish Odonthestes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenesis; Genetically modified fish; Microinjection; Growth hormone cDNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100008

Some historical aspects of plant cytogenetics in Argentina and Uruguay
Provedor de dados: Genetics and Molecular Biology
Autores: Hunziker,Juan H.
A brief account is given of the origin and development of plant cytogenetics in Argentina and Uruguay, along with some of the factors that hampered the development of this area.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400035

Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers
Provedor de dados: Genetics and Molecular Biology
Autores: Silveira,Sheila Recepute; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Sera,Tumoru; Carvalho,Valdemar de Paula; Coelho,Alexandre Siqueira Guedes.
The RAPD technique associated with restriction digestion of genomic DNA was used to assess the genetic variability within and among nine populations of Coffea arabica, including six progenies belonging to the Sarchimor germplasm, the progeny PR 77054-40-10 (Catuaí Vermelho IAC 81 x Icatu), and two commercial cultivars (IAPAR 59 and Catuaí Vermelho IAC-81). These populations were evaluated using analysis of molecular variance (AMOVA), genetic similarity among progenies, and percentage of polymorphic loci. A total of 99 RAPD markers were evaluated of which 67 (67.67%) were polymorphic. AMOVA showed that 38.5% and 61.5% of the genetic variation was distributed among and within populations, respectively. The fixation index (F ST) of the genotypes was 0.385....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee breeding; Genetic variability; Molecular variance; RAPD markers.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300018

QTL affecting body weight in a candidate region of cattle chromosome 5
Provedor de dados: Genetics and Molecular Biology
Autores: Machado,Mariana B.B.; Alencar,Maurício M.; Pereira,Andréa P.; Oliveira,Henrique N.; Casas,Eduardo; Coutinho,Luis L.; Regitano,Luciana C.A.
The objective of this work was to identify QTLs for liveweight in a candidate region of bovine chromosome 5. Half-sib families from two lines, one traditional and the other new, of Canchim beef cattle (5/8 Charolais + 3/8 Zebu) were genotyped for four microsatellite markers, including the microsatellite in the IGF-1 (insulin-like growth factor-1) promoter region. Significant differences in allele distribution between the two lines were found for three markers. Interval mapping analyses in this region indicated the presence of a QTL controlling birth weight (p &lt; 0.05) and of a QTL influencing breeding value for yearling weight (p &lt; 0.01) in the newer line of the breed. The previously identified interaction between the IGF-1 genotype and genetic group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: QTL; Growth traits; Microsatellite; Bovine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300008

Bioinformatics of the sugarcane EST project
Provedor de dados: Genetics and Molecular Biology
Autores: Telles,Guilherme P.; Braga,Marília D.V.; Dias,Zanoni; Tzy-Li,Lin; Quitzau,José A.A.; Silva,Felipe R. da; Meidanis,João.
The Sugarcane EST project (SUCEST) produced 291,904 expressed sequence tags (ESTs) in a consortium that involved 74 sequencing and data mining laboratories. We created a web site for this project that served as a ‘meeting point’ for receiving, processing, analyzing, and providing services to help explore the sequence data. In this paper we describe the information pathway that we implemented to support this project and a brief explanation of the clustering procedure, which resulted in 43,141 clusters.
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100003

Karyotype relationships among Anastrepha bistrigata, A. striata and A. serpentina (Diptera, Tephritidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Selivon,Denise; Sipula,Fabiana Maria; Rocha,Lincoln S.; Perondini,André Luiz P.
The species of Anastrepha are arranged into 17 intrageneric groups. Recently, it was proposed that two species of the striata group, Anastrepha striata and A. bistrigata, might be realocated to serpentina group. Anastrepha bistrigata and A. serpentina have an X1X2Y/X1X1 X2X2 sex chromosome system while A. striata has a XY/XX system. It was previously proposed that the karyotype of A. bistrigata could be derived from that of A. striata by an Y:A fusion, and that the karyotype of A. serpentina would be derived from another, hypothetical karyotype. In the present report sequential staining with DAPI and chromomycin A3 (CMA3), followed by C-banding, revealed that the C-banded heterochromatic blocks of the sex chromosomes of A. bistrigata have different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flies; Mitotic chromosomes; Fluorochromes; Heterochromatin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600009

From yeast to humans: Understanding the biology of DNA Damage Response (DDR) kinases
Provedor de dados: Genetics and Molecular Biology
Autores: Cussiol,José Renato Rosa; Soares,Bárbara Luísa; Oliveira,Francisco Meirelles Bastos de.
Abstract The DNA Damage Response (DDR) is a complex network of biological processes that protect cells from accumulating aberrant DNA structures, thereby maintaining genomic stability and, as a consequence, preventing the development of cancer and other diseases. The DDR pathway is coordinated by a signaling cascade mediated by the PI3K-like kinases (PIKK) ATM and ATR and by their downstream kinases CHK2 and CHK1, respectively. Together, these kinases regulate several aspects of the cellular program in response to genomic stress. Much of our understanding of these kinases came from studies performed in the 1990s using yeast as a model organism. The purpose of this review is to present a historical perspective on the discovery of the DDR kinases in yeast...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Genome instability; DNA damage response; Cell cycle checkpoint; Kinase.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200302

Factors influencing electroporation-mediated gene transfer to Stylosanthes guianensis (Aubl.) Sw. protoplasts
Provedor de dados: Genetics and Molecular Biology
Autores: Quecini,V.M.; Oliveira,C.A. de; Alves,A.C.; Vieira,M.L.C.
In order to develop a high-efficiency and reproducible transformation protocol for Stylosanthes guianensis we assessed the biological and physical parameters affecting plant electroporation protoplasts. Energy input, as combinations of electric field strengths discharged by different capacitors, electroporation buffer and DNA form were evaluated. Transformation efficiency was assayed in vivo as transient reporter gene expression, using the GFP-coding gene mgfp5 driven by a CaMV 35S constitutive promoter. Energy input and electric field strength had a critical influence on transgene expression with higher transformation levels being achieved with 250 V.cm-1 discharged by 900 and 1000 muF capacitors. Linear plasmid DNA, the absence of chloride and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Direct gene transfer; Plant transformation; Forage legume; Stylosanthes guianensis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100014

1p13.2 deletion displays clinical features overlapping Noonan syndrome, likely related to NRAS gene haploinsufficiency
Provedor de dados: Genetics and Molecular Biology
Autores: Linhares,Natália Duarte; Freire,Maíra Cristina Menezes; Cardenas,Raony Guimarães Corrêa do Carmo Lisboa; Pena,Heloisa Barbosa; Lachlan,Katherine; Dallapiccola,Bruno; Bacino,Carlos; Delobel,Bruno; James,Paul; Thuresson,Ann-Charlotte; Annerén,Göran; Pena,Sérgio D. J.
Abstract Deletion-induced hemizygosity may unmask deleterious autosomal recessive variants and be a cause of the phenotypic variability observed in microdeletion syndromes. We performed complete exome sequencing (WES) analysis to examine this possibility in a patient with 1p13.2 microdeletion. Since the patient displayed clinical features suggestive of Noonan Syndrome (NS), we also used WES to rule out the presence of pathogenic variants in any of the genes associated with the different types of NS. We concluded that the clinical findings could be attributed solely to the 1p13.2 haploinsufficiency. Retrospective analysis of other nine reported patients with 1p13.2 microdeletions showed that six of them also presented some characteristics of NS. In all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 1p13.2 deletion; Noonan syndrome type 6; NRAS gene; RASopathy; Unmasking heterozygosity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300349

Is the human species still evolving?: to where?
Provedor de dados: Genetics and Molecular Biology
Autores: Salzano,Francisco M.
The questions of the title have been considered in several ways. First, indications of the traits which make us humans were considered. Then the behavior and culture concepts were examined, and the biology and culture interactions discussed, with an emphasis on the similarities and differences between the genetic and cultural transmissions. Next diverse types of selective pressures were reviewed, and finally pessimistic and optimistic views of our future contrasted. Vigorous action against acts which lead to exclusion and discriminatory policies against human subjects is needed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biology and culture; Genetic selection; Cultural selection; Fluctuating selection; Exploratory behavior.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600002

Oxytocin and arginine vasopressin receptor evolution: implications for adaptive novelties in placental mammals
Provedor de dados: Genetics and Molecular Biology
Autores: Paré,Pamela; Paixão-Côrtes,Vanessa R.; Tovo-Rodrigues,Luciana; Vargas-Pinilla,Pedro; Viscardi,Lucas Henriques; Salzano,Francisco Mauro; Henkes,Luiz E.; Bortolini,Maria Catira.
Abstract Oxytocin receptor (OXTR) and arginine vasopressin receptors (AVPR1a, AVPR1b, and AVPR2) are paralogous genes that emerged through duplication events; along the evolutionary timeline, owing to speciation, numerous orthologues emerged as well. In order to elucidate the evolutionary forces that shaped these four genes in placental mammals and to reveal specific aspects of their protein structures, 35 species were selected. Specifically, we investigated their molecular evolutionary history and intrinsic protein disorder content, and identified the presence of short linear interaction motifs. OXTR seems to be under evolutionary constraint in placental mammals, whereas AVPR1a, AVPR1b, and AVPR2 exhibit higher evolutionary rates, suggesting that they...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxytocin receptor; Arginine vasopressin receptors; Molecular evolution; Protein disorder; Interaction motifs.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400646

Relationship between epistasis and aggressiveness in resistance of pepper (Capsicum annuum L.) to Phytophthora nicotianae
Provedor de dados: Genetics and Molecular Biology
Autores: Bnejdi,Fethi; Saadoun,Morad; Allagui,Mohamed Bechir; Hanbury,Colin; El Gazzah,Mohamed.
This study evaluated the types of gene action governing the inheritance of resistance to Phytophthora nicotianae necrosis in populations derived from two crosses involving two susceptible (Beldi and Nabeul II) and one resistant (CM334) cultivars of pepper (Capsicum annuum L.). Populations, composed of Pr, Ps, F1,F2,BC1Pr, and BC1Ps generations, were inoculated with six P. nicotianae isolates. Generation means analysis indicated that an additive-dominance model was appropriate for P. nicotianae isolates PnKo1,PnKo2 and PnKr1, which showed low aggressiveness in the two crosses. For the more aggressive isolates PnBz1,PnBz2 and PnKr2, epistasis was an integral component of resistance in the two crosses. The presence of epistasis in the resistance of pepper to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Additive model; Best fit model; Gene effect; Heredity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200014

Quantitative trait loci mapping of pubescence density and flowering time of insect-resistant soybean (Glycine max L. Merr.)
Provedor de dados: Genetics and Molecular Biology
Autores: Komatsu,Kunihiko; Okuda,Shiori; Takahashi,Masakazu; Matsunaga,Ryoichi; Nakazawa,Yoshinori.
Analysis of antibiosis resistance to common cutworm (Spodoptera litura Fabricius) in soybean (Glycine max (L.) Merr.) has progressed significantly, but the immediate cause remains unknown. We performed quantitative trait loci (QTL) analysis of pubescence density and plant development stage because these factors are assumed to be the immediate cause of resistance to cutworm. The QTLs for pubescence appeared to be identical to the previously detected the Pd1 and Ps loci controlling pubescence density. We found no candidate loci for flowering time QTLs, although one could be identical to the gene governing the long-juvenile trait or to the E6 loci controlling maturity. None of the QTLs overlapped with the QTLs for antibiosis resistance.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glycine max; Hair; Maturity; Pest resistance; QTL; SSR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400022

Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants
Provedor de dados: Genetics and Molecular Biology
Autores: Homrich,Milena Schenkel; Wiebke-Strohm,Beatriz; Weber,Ricardo Luís Mayer; Bodanese-Zanettini,Maria Helena.
Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes. However, the stable transformation of soybean cannot yet be considered to be routine because it depends on the ability to combine efficient transformation and regeneration techniques. Two methods have been used with relative success to produce completely and stably transformed plants: particle bombardment and the Agrobacterium tumefaciens system. In addition, transformation by Agrobacterium rhizogenes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenic plants; Particle bombardment; Agrobacterium systems; Functional analysis; Genetic improvement.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015

Dietary carotenoid-rich oil supplementation improves exercise-induced anisocytosis in runners: influences of haptoglobin, MnSOD (Val9Ala), CAT (21A/T) and GPX1 (Pro198Leu) gene polymorphisms in dilutional pseudoanemia ("sports anemia")
Provedor de dados: Genetics and Molecular Biology
Autores: Miranda-Vilela,Ana L.; Akimoto,Arthur K.; Alves,Penha C.Z.; Pereira,Luiz C.S.; Klautau-Guimarães,Maria N.; Grisolia,Cesar K.
Physical training induces beneficial adaptation, whereas exhaustive exercises increase reactive oxygen-species generation, thereby causing oxidative damage in plasma and erythrocytes, fractions susceptible to lipid peroxidation. Pequi (Caryocar brasiliense Camb.) is a Brazilian Cerrado fruit containing a carotenoid-rich oil. The aim was to investigate the effects of pequi-oil on exercise-induced oxidative damage in plasma and erythrocytes, after running in the same environment and undergoing weekly training under the same conditions as to type, intensity and length. Evaluations were accomplished after outdoor running on flat land before and after ingestion of 400 mg pequi-oil capsules for 14 days. Blood samples were taken after running and submitted to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caryocar brasiliense Camb hemogram hematimetric indexes (MCV; MCH; MCHC and RDW) haptoglobin antioxidant enzyme polymorphisms.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200026

Chromosome studies of some Vernonia species (Asteraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Dematteis,Massimiliano.
Chromosomes of 12 Vernonia species (Asteraceae) from South America were studied. First counts were reported for: V. alpestris Gardn. (n = 17), V. chalybaea Mart. ex DC. (2n = 32), V. cotoneaster Less. (2n = 30), V. fruticulosa Mart. ex DC. (n = 16) and V. lithospermifolia Hieron. (n = 10, 2n = 20). Furthermore, in V. aurea Mart. ex DC. (2n = 32), V. mollissima D. Don (n = 32) and V. saltensis Hieron. (2n = 64) numbers different from those reported in the literature were found. Karyotypes were described for some species. V. aurea contains 24m + 8sm chromosomes, V. chalybaea contains 22m + 10sm chromosomes, V. cincta Griseb. 36m + 16sm + 8st and V. cotoneaster 18m + 12sm. The basic chromosome numbers x = 10, 14, 15, 16 and 17 were confirmed for South...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300016

Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Veasey,Elizabeth Ann; Borges,Aline; Rosa,Mariana Silva; Queiroz-Silva,Jurema R.; Bressan,Eduardo de Andrade; Peroni,Nivaldo.
We used simple sequence repeat (SSR) markers to investigate the genetic diversity of 78 sweet potato (Ipomoea batatas) accessions (58 landraces and 20 putative clones) from traditional agricultural households from 19 local communities in the Vale do Ribeira, São Paulo, Brazil. Eight SSR loci were assessed using 6% (w/v) polyacrylamide gels stained with silver nitrate and the accessions genotyped considering the presence or absence of bands. The results were subjected to analysis of molecular variance (AMOVA), and cluster and principal coordinate analyses. Spatial structure was assessed using Mantel's test to compare genetic and geographic distances. Each primer pair generated between three and ten clearly scorable polymorphic fragments. Cluster analyses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Landraces; SSR; Traditional agriculture; Vale do Ribeira.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400020

Linkage disequilibrium levels and allele frequency distribution in Blanco Orejinegro and Romosinuano Creole cattle using medium density SNP chip data
Provedor de dados: Genetics and Molecular Biology
Autores: Bejarano,Diego; Martínez,Rodrigo; Manrique,Carlos; Parra,Luis Miguel; Rocha,Juan Felipe; Gómez,Yolanda; Abuabara,Yesid; Gallego,Jaime.
Abstract The linkage disequilibrium (LD) between molecular markers affects the accuracy of genome-wide association studies and genomic selection application. High-density genotyping platforms allow identifying the genotype of thousands of single nucleotide polymorphisms (SNPs) distributed throughout the animal genomes, which increases the resolution of LD evaluations. This study evaluated the distribution of minor allele frequencies (MAF) and the level of LD in the Colombian Creole cattle breeds Blanco Orejinegro (BON) and Romosinuano (ROMO) using a medium density SNP panel (BovineSNP50K_v2). The LD decay in these breeds was lower than those reported for other taurine breeds, achieving optimal LD values (r2 ≥ 0.3) up to a distance of 70 kb in BON and 100...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Creole breeds; BovineSNP50; Linkage disequilibrium; Minor allele frequency.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300426

Microbial diversity of soils on the banks of the Solimões and Negro rivers, state of Amazonas, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Ellen Karla Nobre dos; Honda,Rubens Tomio; Nozawa,Sérgio Ricardo; Ferreira-Nozawa,Monica Stropa.
Analysis of bacterial diversity in soils along the banks of the Solimões and Negro rivers, state of Amazonas, Brazil, was by partial sequencing of the genes codifying the rDNA16S region. Diversity of operational taxonomic units (OTU) and of the divergent sequences obtained were applied in comparative analysis of microbiological diversity in the two environments, based on richness estimators and OTU diversity indices. The higher OTU diversity in the Solimões was based on the higher number of parameters that evoke this. The interaction between the nucleotide sequences of bacteria inhabiting the two riverine environments indicated that the two microrganism communities are similar in composition.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbiological diversity; Tropical soil biodiversity; 16S rDNA gene; Amazon; Molecular biology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100019

Haldane's rule and other aspects of reproductive isolation observed in the Anastrepha fraterculus complex (Diptera: Tephritidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Selivon,Denise; Perondini,André L.P.; Morgante,João S.
Some aspects of reproductive isolation between allopatric populations of two closely related species of the Anastrepha fraterculus complex (A. fraterculus sp. 1 and sp. 2) were evaluated in laboratory conditions. Most of the crosses were fertile in each species as well as between sp. 2 females and sp. 1 males. In the reciprocal cross only 41.7% of the matings yielded viable progeny. Egg hatching occurred at similar rates within the two species, but was significantly lower in the crosses between the species. Adult emergence did not differ significantly among crosses. The sex ratio of adult progeny within each species, as well as in the hybrid progeny derived from sp. 1 females crossed to sp. 2 males, did not differ from the expected 1:1 ratio. However, in...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400007

Diagnosis of patients with Prader-Willi and Angelman Syndromes: the importance of an overall investigation
Provedor de dados: Genetics and Molecular Biology
Autores: Varela,Monica Castro; Fridman,Cintia; Koiffmann,Célia Priszkulnik.
Seventy-two patients with clinical diagnoses of Prader-Willi (PWS; n = 28 patients) or Angelman syndromes (AS; n = 44 patients) were submitted to chromosome analysis, SNRPN-SNURF exon 1 methylation assay, and microsatellite genotyping. Analysis of the methylation pattern confirmed the PWS diagnosis in 18 out of 28 patients and the AS diagnosis in 20 out of 44 patients. FISH and microsatellite analysis detected a deletion in 30 patients (14 PWS and 16 AS). Eight patients had normal FISH results (4 PWS and 4 AS); microsatellite markers showed that these patients had a uniparental disomy (UPD). Based on this study, we propose a strategy for the routine diagnosis of these syndromes that consists of the following steps: 1) methylation analysis, which does not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angelman syndrome; Prader-Willi syndrome; Diagnosis; 15q deletion; Uniparental disomy; Genomic imprinting.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100003

Downregulation of caffeoyl-CoA O-methyltransferase (CCoAOMT) by RNA interference leads to reduced lignin production in maize straw
Provedor de dados: Genetics and Molecular Biology
Autores: Li,Xiaoyu; Chen,Wenjuan; Zhao,Yang; Xiang,Yan; Jiang,Haiyang; Zhu,Suwen; Cheng,Beijiu.
Lignin is a major cell wall component of vascular plants that provides mechanical strength and hydrophobicity to vascular vessels. However, the presence of lignin limits the effective use of crop straw in many agroindustrial processes. Here, we generated transgenic maize plants in which the expression of a lignin biosynthetic gene encoding CCoAOMT , a key enzyme involved in the lignin biosynthesis pathway was downregulated by RNA interference (RNAi). RNAi of CCoAOMT led to significantly downregulated expression of this gene in transgenic maize compared with WT plants. These transgenic plants exhibited a 22.4% decrease in Klason lignin content and a 23.3% increase in cellulose content compared with WT plants, which may reflect compensatory regulation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CCoAOMT; Lignin biosynthesis; Lignin and cellulose content; Maize.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400013

The best of both worlds: Phylogenetic eigenvector regression and mapping
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz Filho,José Alexandre Felizola; Villalobos,Fabricio; Bini,Luis Mauricio.
Eigenfunction analyses have been widely used to model patterns of autocorrelation in time, space and phylogeny. In a phylogenetic context, Diniz-Filho et al. (1998) proposed what they called Phylogenetic Eigenvector Regression (PVR), in which pairwise phylogenetic distances among species are submitted to a Principal Coordinate Analysis, and eigenvectors are then used as explanatory variables in regression, correlation or ANOVAs. More recently, a new approach called Phylogenetic Eigenvector Mapping (PEM) was proposed, with the main advantage of explicitly incorporating a model-based warping in phylogenetic distance in which an Ornstein-Uhlenbeck (O-U) process is fitted to data before eigenvector extraction. Here we compared PVR and PEM in respect to...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Evolutionary models; Phylogenetic comparative methods; Phylogenetic imputation; Phylogenetic signal.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300396

Growth Hormone insensitivity (Laron syndrome): Report of a new family and review of Brazilian patients
Provedor de dados: Genetics and Molecular Biology
Autores: Villela,Thais R.; Freire,Bruna L.; Braga,Nathalia T. P.; Arantes,Rodrigo R.; Funari,Mariana F. A.; Alexander,Jorge A L; Silva,Ivani N.
Abstract Laron’s syndrome (LS) is a rare genetic disorder characterized by insensitivity to growth hormone (GH). Up to the present time, over 70 mutations of GH receptor (GHR) gene have been identified leading to GH/insulin-like growth factor type 1 (IGF1) signaling pathway defect. The number of LS patients worldwide is unknown, as many are probably undiagnosed. We report two sibs from a consanguineous family from Minas Gerais, southeastern Brazil. The parents have three children. The older, a 4-years-old girl was 80.2 cm tall (-5.7 SDS height/age), and the youngest sister, aged 3 years, was 73.2 cm tall (-5.82 SDS height/age). Their clinical and biochemical features are typical of LS patients, such as high serum level of GH and low IGF1 concentrations. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laron Syndrome; Growth hormone; Growth hormone receptor; Genetics.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500102

Growth and carcass traits associated with GH1/Alu I and POU1F1/Hinf I gene polymorphisms in Zebu and crossbred beef cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Curi,Rogério A.; Palmieri,Darío A.; Suguisawa,Liliane; Oliveira,Henrique N. de; Silveira,Antonio C.; Lopes,Catalina R.
The objectives of the present study were to estimate the allele and genotype frequencies of the GH1/Alu I and POU1F1/Hinf I polymorphisms in beef cattle belonging to different genetic groups and to determine the effects of these polymorphisms on growth and carcass traits in cattle submitted to feedlot management, an intensive production model. Genotyping was performed on 384 animals, including 79 Nellore, 30 Canchim (5/8 Charolais + 3/8 Zebu), 30 Simmental x Nellore crossbred and 245 Angus x Nellore crossbred cattle. Body weight, weight gain, dressing percentage, Longissimus dorsi area and backfat thickness were fitted using the General Linear Model (GLM) procedure of the SAS program and the least square means of the genotypes were compared using the F...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Polymorphisms; Candidate gene; Growth; Carcass.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100012

RNA-binding proteins and their role in the regulation of gene expression in Trypanosoma cruzi and Saccharomyces cerevisiae
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Camila; Faoro,Helisson; Alves,Lysangela Ronalte; Goldenberg,Samuel.
Abstract RNA-binding proteins (RBPs) have important functions in the regulation of gene expression. RBPs play key roles in post-transcriptional processes in all eukaryotes, such as splicing regulation, mRNA transport and modulation of mRNA translation and decay. RBPs assemble into different mRNA-protein complexes, which form messenger ribonucleoprotein complexes (mRNPs). Gene expression regulation in trypanosomatids occurs mainly at the post-transcriptional level and RBPs play a key role in all processes. However, the functional characterization of RBPs in Trypanosoma cruzi has been impaired due to the lack of reliable reverse genetic manipulation tools. The comparison of RBPs from Saccharomyces cerevisiae and T. cruzi might allow inferring on the function...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA-binding proteins; Trypanosoma cruzi; Saccharomyces cerevisiae; Gene expression regulation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100022

Cytogenetic markers as diagnoses in the identification of the hybrid between Piauçu (Leporinus macrocephalus) and Piapara (Leporinus elongatus)
Provedor de dados: Genetics and Molecular Biology
Autores: Porto-Foresti,Fábio; Hashimoto,Diogo Teruo; Alves,Anderson Luis; Almeida,Rodrigo Braz Castilho; Senhorini,José Augusto; Bortolozzi,Jehud; Foresti,Fausto.
The genetic monitoring of interspecific hybrids involves the application of methodologies able to provide an easy and indubitable genetic characterization of both parental and hybrid individuals. In the present work, cytogenetic techniques were used to identify a hybrid lineage of "Piaupara" in order to caracterize them in relation to the parental species, Leporinus macrocephalus (piauçu) and L. elongatus (piapara). The cytogenetic analysis revealed that L. macrocephalus presented 2n = 54 chromosomes and a nucleolar organizer regions (NOR) at the telomere of the long arm of the submetacentric chromosome pair 2. Analysis of constitutive heterochromatin (C-banding) revealed a conspicuous block at the pericentromeric region on the long arm of a submetacentric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interspecific hybrid; Fish culture; Fish cytogenetics.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200005

The third of a series of articles for the 60th anniversary of the Brazilian Society of Genetics
Provedor de dados: Genetics and Molecular Biology
Autores: Salzano,Francisco Mauro; Santos,Fabrício Rodrigues dos; Hartfelder,Klaus; Menck,Carlos F.M.
Tipo: Info:eu-repo/semantics/other
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100001

Mitochondrial DNA common deletion is not associated with thyroid, breast and colorectal tumors in Turkish patients
Provedor de dados: Genetics and Molecular Biology
Autores: Aral,Cenk; Akkiprik,Mustafa; Kaya,Handan; Ataizi-Çelikel,Çigdem; Çaglayan,Sinan; Özisik,Gökhan; Baloglu,Hüseyin; Özer,Ayse.
Recently, efforts have been focused on mitochondrial DNA changes and their relation to human cancers. Among them, a 4977 bp deletion of mitochondrial DNA, named "common deletion", has been investigated in several types of tumors, with inconsistent results. In this study, we investigated the presence of the common deletion in tissues from 25 breast, 25 colorectal and 50 thyroid tumors and in the adjacent healthy tissues from Turkish patients. Samples from healthy volunteers were also evaluated for comparison. Two PCR-based methods were used for the detection of the common deletion. First, two pairs of primers were used to amplify wild-type and deleted mtDNA. Then, a highly sensitive nested-PCR was performed, to determine low amounts of deleted genomes. By...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial DNA; Common deletion; Cancer.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100001

Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Palmieri,Darío A.; Bechara,Marcelo D.; Curi,Rogério A.; Monteiro,Jomar P.; Valente,Sérgio E.S.; Gimenes,Marcos A.; Lopes,Catalina R.
Diversity in 26 microsatellite loci from section Caulorrhizae germplasm was evaluated by using 33 accessions of A. pintoi Krapov. &amp;amp; W.C. Gregory and ten accessions of Arachis repens Handro. Twenty loci proved to be polymorphic and a total of 196 alleles were detected with an average of 9.8 alleles per locus. The variability found in those loci was greater than the variability found using morphological characters, seed storage proteins and RAPD markers previously used in this germplasm. The high potential of these markers to detect species-specific alleles and discriminate among accessions was demonstrated. The set of microsatellite primer pairs developed by our group for A. pintoi are useful molecular tools for evaluating Section Caulorrhizae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachis; Genetic diversity; Germplasm; Microsatellites; Molecular markers.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100019

CYP3A5 genotyping for assessing the efficacy of treatment with simvastatin and atorvastatin
Provedor de dados: Genetics and Molecular Biology
Autores: Kolovou,Genovefa; Kolovou,Vana; Ragia,Georgia; Mihas,Constantinos; Diakoumakou,Olga; Vasiliadis,Ioannis; Mavrogeni,Sophie; Vartela,Vassiliki; Manolopoulos,Vangelis G.
In this work, we examined the impact of polymorphism in the cytochrome P450 (CYP) 3A5 gene, CYP3A5*1 (6986A &gt; G, rs 776746), on the reduction in the lipid levels caused by simvastatin and atorvastatin. We studied 350 hyperlipidemic patients who received 10-40 mg of atorvastatin (n = 175) or simvastatin (n = 175) daily. Genotyping for CYP3A5 was done by PCR-RFLP analysis. Differences in the lipid profile before and after treatment were expressed as the % difference. The frequency of CYP3A5 polymorphism was 13.4% for heterozygotes and 86.6% for homozygotes. Comparison of the responses to same dose of each drug showed that the highest % difference was associated with total cholesterol (TC) in subjects receiving atorvastatin 40 mg compared with simvastatin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atorvastatin; Cholesterol; CYP3A5 gene polymorphism; Simvastatin.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200129

Cloning, expression and characterization of alcohol dehydrogenases in the silkworm Bombyx mori
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,Nan; Shi,Haifeng; Yao,Qin; Zhou,Yang; Kang,Lequn; Chen,Huiqin; Chen,Keping.
Alcohol dehydrogenases (ADH) are a class of enzymes that catalyze the reversible oxidation of alcohols to corresponding aldehydes or ketones, by using either nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP), as coenzymes. In this study, a short-chain ADH gene was identified in Bombyx mori by 5'-RACE PCR. This is the first time the coding region of BmADH has been cloned, expressed, purified and then characterized. The cDNA fragment encoding the BmADH protein was amplified from a pool of silkworm cDNAs by PCR, and then cloned into E. coli expression vector pET-30a(+). The recombinant His-tagged BmADH protein was expressed in E. coli BL21 (DE3), and then purified by metal chelating affinity chromatography. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5'-RACE PCRADH; Enzymatic activity; Recombinant protein.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200013

Plant polytene chromosomes
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalheira,Gianna Maria Griz.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400050

A transcriptome analysis of mitten crab testes (Eriocheir sinensis)
Provedor de dados: Genetics and Molecular Biology
Autores: Zhang,Wei; Wan,Haolei; Jiang,Hui; Zhao,Yunlong; Zhang,Xiaowei; Hu,Songnian; Wang,Qun.
The identification of expressed genes involved in sexual precocity of the mitten crab (Eriocheir sinensis) is critical for a better understanding of its reproductive development. To this end, we constructed a cDNA library from the rapid developmental stage of testis of E. sinensis and sequenced 3,388 randomly picked clones. After processing, 2,990 high-quality expressed sequence tags (ESTs) were clustered into 2,415 unigenes including 307 contigs and 2,108 singlets, which were then compared to the NCBI non-redundant (nr) protein and nucleotide (nt) database for annotation with Blastx and Blastn, respectively. After further analysis, 922 unigenes were obtained with concrete annotations and 30 unigenes were found to have functions possibly related to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reproduction; Testis; EST; Eriocheir sinensis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100024

Characterization of the core microbiota of the drainage and surrounding soil of a Brazilian copper mine
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Letícia Bianca; Vicentini,Renato; Ottoboni,Laura M.M.
Abstract The core microbiota of a neutral mine drainage and the surrounding high heavy metal content soil at a Brazilian copper mine were characterized by 16S rDNA pyrosequencing. The core microbiota of the drainage was dominated by the generalist genus Meiothermus. The soil samples contained a more heterogeneous bacterial community, with the presence of both generalist and specialist bacteria. Both environments supported mainly heterotrophic bacteria, including organisms resistant to heavy metals, although many of the bacterial groups identified remain poorly characterized. The results contribute to the understanding of bacterial communities in soils impacted by neutral mine drainage, for which information is scarce, and demonstrate that heavy metals can...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mine drainage; Soil; 16S rDNA pyrosequencing; Core microbiota; Generalist and specialist OTUs.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400484

Leaf-associated bacterial microbiota of coffee and its correlation with manganese and calcium levels on leaves
Provedor de dados: Genetics and Molecular Biology
Autores: Sousa,Leandro Pio de; Silva,Marcio José da; Costa Mondego,Jorge Maurício.
Abstract Coffee is one of the most valuable agricultural commodities and the plants’ leaves are the primary site of infection for most coffee diseases, such as the devastating coffee leaf rust. Therefore, the use of bacterial microbiota that inhabits coffee leaves to fight infections could be an alternative agricultural method to protect against coffee diseases. Here, we report the leaf-associated bacteria in three coffee genotypes over the course of a year, with the aim to determine the diversity of bacterial microbiota. The results indicate a prevalence of Enterobacteriales in Coffea canephora, Pseudomonadales in C. arabica ‘Obatã’, and an intriguing lack of bacterial dominance in C. arabica ‘Catuaí’. Using PERMANOVA analyses, we assessed the association...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Bacteria; 16S; Leaf; Manganese; Calcium.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300455

Karyological study of Amphisbaena ridleyi (Squamata, Amphisbaenidae), an endemic species of the Archipelago of Fernando de Noronha, Pernambuco, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Laguna,Marcia Maria; Amaro,Renata Cecília; Mott,Tamí; Yonenaga-Yassuda,Yatiyo; Rodrigues,Miguel Trefaut.
The karyotype of Amphisbaena ridleyi, an endemic species of the archipelago of Fernando de Noronha, in State of Pernambuco, Brazil, is described after conventional staining, Ag-NOR impregnation and fluorescence in situ hybridization (FISH) with a telomeric probe. The diploid number is 46, with nine pairs of macrochromosomes (three metacentrics, four subtelocentrics and two acrocentrics) and 14 pairs of microchromosomes. The Ag-NOR is located in the telomeric region of the long arm of metacentric chromosome 2 and FISH revealed signals only in the telomeric region of all chromosomes. Further cytogenetic data on other amphisbaenians as well as a robust phylogenetic hypothesis of this clade is needed in order to understand the evolutionary changes on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphisbaena ridleyi; Karyotype; Fernando de Noronha; Ag-NOR; FISH with telomeric probes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100011

Combined genotypes of the MBL2 gene related to low mannose-binding lectin levels are associated with vaso-occlusive events in children with sickle cell anemia
Provedor de dados: Genetics and Molecular Biology
Autores: Medeiros,Fernanda Silva; Mendonça,Taciana Furtado de; Lopes,Katiuscia Araújo de Miranda; França,Laís Medeiros da Câmara; Silva,Andreia Soares da; Vasconcelos,Luydson Richardson Silva; Oliveira,Maria do Carmo Valgueiro Costa de; Anjos,Ana Cláudia Mendonça dos; Hatzlhofer,Betânia Lucena Domingues; Bezerra,Marcos André Cavalcanti; Araújo,Aderson da Silva; Moura,Patrícia; Cavalcanti,Maria do Socorro de Mendonça.
Abstract Sickle cell anemia (SCA) presents heterogenous clinical manifestations that cannot be explained solely by alterations to hemoglobin (Hb); other components such as endothelial adhesion, thrombosis and inflammation may be involved. The mannose-binding lectin (MBL) has an important role in innate immunity and inflammatory diseases. In this report, we describe an association between MBL2 polymorphism related to low production of serum MBL and the frequency of vasoocclusive events (FVOE) in children ≤ 5 years old with SCA (p = 0.0229; OR 5.55; CI 1.11-27.66). Further studies are needed to explore the role of low MBL2 in the pathophysiology of vasoocclusive events in SCA.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MBL2; Polymorphism; Sickle cell anemia; Vaso-occlusive events.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400600

Non-random distribution of high density chromatin detected at opposite ends of T-banded human metaphase chromosomes
Provedor de dados: Genetics and Molecular Biology
Autores: Santiñaque,Federico F.; Drets,Máximo E.
Previous research using microdensitometric scanning and computer graphic image analysis showed that T-banded segments of human metaphase chromosomes usually exhibit an asymmetrical distribution of high density (HD) chromatin between sister chromatids. Here, we employed the same methods to analyze HD chromatin distribution at opposite ends of T-banded human lymphocyte chromosomes. This study revealed that in most chromosomes with an asymmetrical distribution of HD chromatin at both ends, the highest densities of each arm were located in opposite chromatids. The frequency of this configuration was 0.792 per chromosome, indicating that the highest chromatin densities of the terminal segments of T-banded human chromosomes were non-randomly distributed at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human chromosomes; T-banding; Chromatin structure; Telomere; Microdensitometry.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600004

Expression analysis in response to drought stress in soybean: shedding light on the regulation of metabolic pathway genes
Provedor de dados: Genetics and Molecular Biology
Autores: Guimarães-Dias,Fábia; Neves-Borges,Anna Cristina; Viana,Antonio Americo Barbosa; Mesquita,Rosilene Oliveira; Romano,Eduardo; Grossi-de-Sá,Maria de Fátima; Nepomuceno,Alexandre Lima; Loureiro,Marcelo Ehlers; Alves-Ferreira,Márcio.
Metabolomics analysis of wild type Arabidopsis thaliana plants, under control and drought stress conditions revealed several metabolic pathways that are induced under water deficit. The metabolic response to drought stress is also associated with ABA dependent and independent pathways, allowing a better understanding of the molecular mechanisms in this model plant. Through combining an in silico approach and gene expression analysis by quantitative real-time PCR, the present work aims at identifying genes of soybean metabolic pathways potentially associated with water deficit. Digital expression patterns of Arabidopsis genes, which were selected based on the basis of literature reports, were evaluated under drought stress condition by Genevestigator. Genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Drought resistance; QPCR; Metabolic pathway; Bioinformatics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200004

Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
Provedor de dados: Genetics and Molecular Biology
Autores: Paiva,Marcelo Henrique Santos; Guedes,Duschinka Ribeiro Duarte; Leal,Walter Soares; Ayres,Constância Flávia Junqueira.
Abstract Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (&lt; 106 RNA copies/ml).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ZIKV; RT-PCR; Aedes aegypti; Vector competence.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400597

Spatiotemporal expression of MYD88 gene in pigs from birth to adulthood
Provedor de dados: Genetics and Molecular Biology
Autores: Gan,LiNa; Qin,WeiYun; Wu,Sen; Wu,ShengLong; Bao,WenBin.
Abstract MYD88 plays an important role in the immune response against infections. To analyze MYD88 gene expression during different stages of pig development, we used real-time PCR. MYD88 was seen expressed in all tissues examined. MYD88 expression in spleen, lungs, and thymus reached its highest value from 7 to 14 days of age and decreased thereafter. Expression in lymph nodes was high until 28 days of age and then it declined after weaning, with stable low levels in adult pigs. MYD88 expression was high before 35 days of age in the small intestine (duodenum, jejunum, and ileum), where it reached its highest value from 7 to 14 days of age. MYD88 expression in the small intestine declined post-weaning and remained relatively low during adulthood. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MYD88; Pig; Developmental expression; Immune response.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100119

Cytogenetic studies in Phaseolus L. (Fabaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Mercado-Ruaro,Pedro; Delgado-Salinas,Alfonso.
A review of the cytogenetic studies carried out on Phaseolus as well as the different proposals that have been suggested to explain the chromosomal changes in the group are presented. The importance of including wild species in cytogenetic studies and the collaboration between taxonomists and cytogeneticists in order to draw better conclusions are emphasized.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400043

Identification of potential target genes of USP22 via ChIP-seq and RNA-seq analysis in HeLa cells
Provedor de dados: Genetics and Molecular Biology
Autores: Gong,Zhen; Liu,Jianyun; Xie,Xin; Xu,Xiaoyuan; Wu,Ping; Li,Huimin; Wang,Yaqin; Li,Weidong; Xiong,Jianjun.
Abstract The ubiquitin-specific protease 22 (USP22) is an oncogene and its expression is upregulated in many types of cancer. In the nucleus, USP22 functions as one subunit of the SAGA to regulate gene transcription. However, the genome-wide USP22 binding sites and its direct target genes are yet clear. In this study, we characterized the potential genomic binding sites of UPS22 and GCN5 by ChIP-seq using specific antibodies in HeLa cells. There were 408 overlapping putative target genes bound by both USP22 and GCN5. Motif analysis showed that the sequences bound by USP22 and GCN5 shared two common motifs. Gene ontology (GO) and pathway analysis indicated that the genes targeted by USP22 and GCN5 were involved in different physiological processes and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: USP22; Target genes; ChIP-seq; Knockdown; RNA-seq.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300488

Polymorphism of locus DRB3.2 in populations   of Creole Cattle from Northern Mexico
Provedor de dados: Genetics and Molecular Biology
Autores: Fernández,Ilda G.; Ramírez,José Gonzalo Ríos; Vázquez,Amanda Gayosso; Arvizu,Raúl Ulloa; Morales,Rogelio A. Alonso.
The polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in two northern Mexican Creole cattle populations, Chihuahua (n = 47) and Tamaulipas (n = 51). The BoLA-DRB3.2 locus was typed by amplification and digestion with restriction endonuclease enzymes (PCR-RFLP). Fifty-two alleles were detected (28 previously reported and 24 new ones). In the Chihuahua population, 18 alleles and 5.5 effective alleles were found, while in the Tamaulipas population there were 34 and 10.8, respectively. The allele frequencies ranged from 0.011 to 0.383 in Chihuahua and from 0.010 to 0.206 in Tamaulipas. The frequencies of the new alleles in both cattle populations were low (0.010 to 0.053). The expected heterozygosity was 0.827 and 0.916,...
Palavras-chave: BoLA-DRB3.2; Polymorphism; Creole cattle; PCR-RFLP.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500013

High levels of genetic variability and differentiation in hilsa shad, Tenualosa ilisha (Clupeidae, Clupeiformes) populations revealed by PCR-RFLP analysis of the mitochondrial DNA D-loop region
Provedor de dados: Genetics and Molecular Biology
Autores: Mazumder,Sabuj Kanti; Alam,Md. Samsul.
The hilsa shad, Tenualosa ilisha (Clupeidae, Clupeiformes) is an important anadromous clupeid species from the Western division of the Indo-Pacific region. It constitutes the largest single fishable species in Bangladesh. Information on genetic variability and population structure is very important for both management and conservation purposes. Past reports on the population structure of T. ilisha involving morphometric, allozyme and RAPD analyses are contradictory. We examined genetic variability and divergence in two riverine (the Jamuna and the Meghna), two estuarine (Kuakata and Sundarbans) and one marine (Cox's Bazar) populations of T. ilisha by applying PCR-RFLP analysis of the mtDNA D-loop region. The amplified PCR products were restricted with four...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; PCR-RFLP; MtDNA; Tenualosa ilisha.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100029

Prediction of binding hot spot residues by using structural and evolutionary parameters
Provedor de dados: Genetics and Molecular Biology
Autores: Higa,Roberto Hiroshi; Tozzi,Clésio Luis.
In this work, we present a method for predicting hot spot residues by using a set of structural and evolutionary parameters. Unlike previous studies, we use a set of parameters which do not depend on the structure of the protein in complex, so that the predictor can also be used when the interface region is unknown. Despite the fact that no information concerning proteins in complex is used for prediction, the application of the method to a compiled dataset described in the literature achieved a performance of 60.4%, as measured by F-Measure, corresponding to a recall of 78.1% and a precision of 49.5%. This result is higher than those reported by previous studies using the same data set.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hot spots prediction; Protein structure; Hot spots.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300029

The cuttable C-related genotype and allele for the E-cadherin 3’-UTR Pml I polymorphism are associated with higher susceptibility to endometriosis
Provedor de dados: Genetics and Molecular Biology
Autores: Hsieh,Yao-Yuan; Chang,Chi-Chen; Tsai,Fuu-Jen; Hsu,Chin-Moo; Lin,Cheng-Chieh; Tsai,Chang-Hai.
Epithelial cadherin (E-cadherin; CDH1) may influence pericellular proteolysis and intracellular signal transduction, which plays an essential part of tumor invasion. In our study we investigated the correlation between CDH1 gene polymorphism and endometriosis in two groups of pre-menopausal Taiwanese women, group 1 (n = 150) consisting of women with severe stage IV endometriosis and group 2 (n = 159) of women with no endometriosis. The polymerase chain reaction (PCR) was used to identify the cuttable (C) and uncuttable (T) polymorphism of the CDH1-Pml I gene (rs1801026) located on the 3’-untranslated region (3’-UTR) of chromosome 16 and compare the genotypes and allelic frequencies of this gene in both groups. We found that the genotype and allele...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Endometriosis; E-cadherin; Polymorphism; SNP.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500003

Heterochromatin patterns and karyotype relationships within and between the genera Brycon and Salminus (Pisces, Characidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Margarido,Vladimir Pavan; Galetti Junior,Pedro Manoel.
Chromosomes of two Brycon species (B. lundii and B. microlepis) and Salminus hilarii were analyzed. Based on constitutive heterochromatin distribution patterns, karyotypic relationships within and between Bryconinae and Salmininae were examined. A monophyletic origin for the genus Brycon, comprising at least two chromosome synapomorphies (presence of two large submetacentric bearing paracentromeric and telomeric heterochromatin), is suggested. Moreover, Bryconinae and Salmininae may represent a monophyletic unit among Characidae, as they share several chromosome features.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300012

Aromatase gene and its effects on growth, reproductive and maternal ability traits in a multibreed sheep population from Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Lôbo,Ana Maria Bezerra Oliveira; Lôbo,Raimundo Nonato Braga; Paiva,Samuel Rezende.
We determined the polymorphism C242T of the aromatase gene (Cyp19) and its allelic frequency, as well as the effect of the variants on productive and reproductive traits in 71 purebred Santa Inês sheep, 13 purebred Brazilian Somali sheep, nine purebred Poll Dorset sheep, and 18 crossbred 1/2 Dorper sheep. The animals were genotyped using the PCR-RFLP technique. The influence of the animal's genotype on its performance or on the performance of its lambs was analyzed by the least square method. Another factor assessed was the importance of the animal's genotype in analysis models for quantitative breeding value estimates, and whether there were differences among the averages of breeding values of animals with different genotypes for this gene. In the sample...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Body weight; Lambing interval; PCR-RFLP; Polymorphism; SNP; Litter weight.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300010

The parametric restrictions of the Gardner and Eberhart diallel analysis model: heterosis analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Viana,José Marcelo Soriano.
It was studied the parametric restrictions of the diallel analysis model of Griffing, method 2 (parents and F1 generations) and model 1 (fixed), in order to address the questions: i) does the statistical model need to be restricted? ii) do the restrictions satisfy the genetic parameter values? and iii) do they make the analysis and interpretation easier? Objectively, these questions can be answered as: i) yes, ii) not all of them, and iii) the analysis is easier, but the interpretation is the same as in the model with restrictions that satisfy the parameter values. The main conclusions were that: the statistical models for combining ability analysis are necessarily restricted; in the Griffing model (method 2, model 1), the restrictions relative to the...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400028

Screening cotton genotypes for seedling  drought tolerance
Provedor de dados: Genetics and Molecular Biology
Autores: Penna,Julio C. Viglioni; Verhalen,Laval M.; Kirkham,M.B.; McNew,Ronald W.
The objectives of this study were to adapt a screening method previously used to assess seedling drought tolerance in cereals for use in cotton (Gossypium hirsutum L.) and to identify tolerant accessions among a wide range of genotypes. Ninety genotypes were screened in seven growth chamber experiments. Fifteen-day-old seedlings were subjected to four 4-day drought cycles, and plant survival was evaluated after each cycle. Three cycles are probably the minimum required in cotton work. Significant differences (at the 0.05 level or lower) among entries were obtained in four of the seven experiments. A "confirmation test" with entries previously evaluated as "tolerant" (high survival) and "susceptible" (low survival) was run. A number of entries duplicated...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400023

Glutathione S-transferase M1 (GSTM1) polymorphism in two Brazilian populations
Provedor de dados: Genetics and Molecular Biology
Autores: Hatagima,Ana; Klautau-Guimarães,Maria Nazaré; Silva,Felizardo Penalva da; Cabello,Pedro Hernan.
The distribution of GSTM1 phenotype frequencies was studied in two Brazilian samples composed of healthy and unrelated blood donors of both sexes ranging in age from 18 to 61 years. The first sample consisted of 658 individuals from Rio de Janeiro, and the second included 179 individuals from Brasília. The GSTM1 phenotypes were detected using PCR reactions and subsequent digestion by the restriction enzyme HaeII. The GSTM1 null phenotype frequency was 46% and 49% for Rio de Janeiro and Brasília samples, respectively. The GSTM1 phenotype distributions were not in agreement with Hardy-Weinberg equilibrium in either sample, chi²1 = 11.49 (P &lt; 0.001) for Rio de Janeiro and chi²1 = 6.77 (P &lt; 0.01) for Brasília. This deviation from Hardy-Weinberg...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400003

The Hardy-Weinberg principle
Provedor de dados: Genetics and Molecular Biology
Autores: Stark,Alan E.
Hardy-Weinberg genotypic proportions can be maintained in a population under non-random mating. A compact formula gives the proportions of mating pair types. These are illustrated by some simple examples.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hardy-Weinberg equilibrium; Non-random mating.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300027

Characterization of the third SERK gene in pineapple (Ananas comosus) and analysis of its expression and autophosphorylation activity in vitro
Provedor de dados: Genetics and Molecular Biology
Autores: Ma,Jun; He,Yehua; Hu,Zhongyi; Xu,Wentian; Xia,Jingxian; Guo,Cuihong; Lin,Shunquan; Chen,Chengjie; Wu,Chenghou; Zhang,Junli.
Two somatic embryogenesis receptor-like kinase genes (identified as AcSERK1 and AcSERK2) have previously been characterized from pineapple (Ananas comosus). In this work, we describe the characterization of a third gene (AcSERK3) in this family. AcSERK3 had all the characteristic domains and shared extensive sequence homology with other plant SERKs. AcSERK3 expression was studied by in situ hybridization and quantitative real-time PCR to analyze its function. Intense in situ hybridization signals were observed only in single competent cells and competent cell clusters; no hybridization signal was detected in the subsequent stages of somatic embryogenesis. AcSERK3 was highly expressed in embryogenic callus compared to other organs, e.g., 20-80 fold more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AcSERK3; Autophosphorylation activity; Expression analysis; Prokaryotic expression; Somatic embryogenesis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400009

Infrequent V617F mutation of the JAK2 gene in myeloid leukemia and its absence in lymphoid malignancies in Japan
Provedor de dados: Genetics and Molecular Biology
Autores: Mori,Naoki; Yoshinaga,Kentaro; Tada,Makiko; Wang,Yanhua; Shiseki,Masayuki; Motoji,Toshiko.
A unique mutation of the JAK2 gene, V617F, has recently been identified in polycythemia vera, essential thrombocythemia and myeloid metaplasia with myelofibrosis. To determine the relevance of this mutation in other types of hematological neoplasms in Japan, we performed allele-specific polymerase chain reaction analysis on the JAK2 gene. The V617F mutation was detected in one out of 130 myeloid neoplasms, but in none of 114 lymphoid malignancies and four biphenotypic acute leukemias. Although a favorable chromosomal alteration t(8;21)(q22;q22) was observed in one acute myeloid leukemia (AML) patient with the mutation, two courses of chemotherapy resulted in induction failure and short survival. Sequencing of JAK2 cDNA revealed expression of the mutant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: JAK2 gene; V617F mutation; Signal transduction; Acute myeloid leukemia; Lymphoid malignancies.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300006

Genetic variability in the endophytic fungus Guignardia citricarpa isolated from citrus plants
Provedor de dados: Genetics and Molecular Biology
Autores: Glienke-Blanco,Chirlei; Aguilar-Vildoso,Carlos Ivan; Vieira,Maria Lúcia Carneiro; Barroso,Paulo Augusto Vianna; Azevedo,João Lúcio.
During some phases of of their life-cycle endophytic fungi colonize plants asymptomatically being found most frequently inside the aerial part of plant tissues. After surface disinfection of apparently healthy leaves from three varieties of mandarin orange and one tangor, and after incubation on appropriate culture medium, 407 fungal isolates were obtained, giving a total infection frequency of 81%. No fungal growth was observed from disinfected seeds, indicating that fungi are probably not transmitted via seeds. Of the fungal isolates, 27% belonged to the genus Guignardia, with 12 isolates being identified as Guignardia citricarpa Kiely, which is described as a citrus pathogen. The isolates were variable in respect to the presence of sexual structures and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Endophytes; Genetic variability; Guignardia citricarpa; Mandarin; RAPD.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200021

Molecular evolution and transcriptional profile of GH3 and GH20 β-N-acetylglucosaminidases in the entomopathogenic fungus Metarhizium anisopliae
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Eder Silva de; Junges,Ângela; Sbaraini,Nicolau; Andreis,Fábio Carrer; Thompson,Claudia Elizabeth; Staats,Charley Christian; Schrank,Augusto.
Abstract Cell walls are involved in manifold aspects of fungi maintenance. For several fungi, chitin synthesis, degradation and recycling are essential processes required for cell wall biogenesis; notably, the activity of β-N-acetylglucosaminidases (NAGases) must be present for chitin utilization. For entomopathogenic fungi, such as Metarhizium anisopliae, chitin degradation is also used to breach the host cuticle during infection. In view of the putative role of NAGases as virulence factors, this study explored the transcriptional profile and evolution of putative GH20 NAGases (MaNAG1 and MaNAG2) and GH3 NAGases (MaNAG3 and MaNAG4) identified in M. anisopliae. While MaNAG2 orthologs are conserved in several ascomycetes, MaNAG1 clusters only with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NAGases; GH20 and GH3; Metarhizium; Chitinolytic system; Entomopathogenesis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500843

Introduction of the rd29A: AtDREB2A CA gene into soybean (Glycine max L. Merril) and its molecular characterization in leaves and roots during dehydration
Provedor de dados: Genetics and Molecular Biology
Autores: Engels,Cibelle; Fuganti-Pagliarini,Renata; Marin,Silvana Regina Rockenbach; Marcelino-Guimarães,Francismar Corrêa; Oliveira,Maria Cristina Neves; Kanamori,Norihito; Mizoi,Junya; Nakashima,Kazuo; Yamaguchi-Shinozaki,Kazuko; Nepomuceno,Alexandre Lima.
The loss of soybean yield to Brazilian producers because of a water deficit in the 2011-2012 season was 12.9%. To reduce such losses, molecular biology techniques, including plant transformation, can be used to insert genes of interest into conventional soybean cultivars to produce lines that are more tolerant to drought. The abscisic acid (ABA)-independent Dehydration Responsive Element Binding (DREB) gene family has been used to obtain plants with increased tolerance to abiotic stresses. In the present study, the rd29A:AtDREB2A CA gene from Arabidopsis thaliana was inserted into soybean using biolistics. Seventy-eight genetically modified (GM) soybean lines containing 2-17 copies of the AtDREB2A CA gene were produced. Two GM soybean lines (P1397 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arabidopsis; Differential expression; Genetically modified organism; Water deficit.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400015

The combined risk effect among BIN1, CLU, and APOE genes in Alzheimer’s disease
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Lígia Ramos dos; Almeida,Jucimara Ferreira Figueiredo; Pimassoni,Lúcia Helena Sagrillo; Morelato,Renato Lírio; Paula,Flavia de.
Abstract Genome-wide associations studies (GWAS) are detecting new variants associated with late-onset of Alzheimer’s disease (LOAD), a multifactorial neurodegenerative disorder. The variants rs744373 BIN1, rs11136000 CLU and rs3764650 ABCA7 uncovered by GWAS led to different AD pathways, such as metabolism, trafficking and endocytosis of lipids and inflammation. However, most of the association studies did not replicate these variants with significance. This could be due to a small power effect evident when these variants are tested independently with LOAD. Therefore, we aimed to investigate whether the combination of different variants would additively modify the risk of association with LOAD that is observed in GWAS. We performed an association study...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GWAS variants; APOE; CLU; BIN1; ABCA7.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100113

Minimum number and best combinations of harvests to evaluate accessions of tomato plants from germplasm banks
Provedor de dados: Genetics and Molecular Biology
Autores: Abreu,Flávia Barbosa; Silva,Derly José Henriques da; Marim,Bruno Garcia; Carneiro,Pedro Crescêncio Souza; Juhasz,Ana Cristina Pinto; De Luca,Carina A.C.; Valente,Reginaldo Fialho; Guimarães,Marcelo Almeida.
This study presents the minimum number and the best combination of tomato harvests needed to compare tomato accessions from germplasm banks. Number and weight of fruit in tomato plants are important as auxiliary traits in the evaluation of germplasm banks and should be studied simultaneously with other desirable characteristics such as pest and disease resistance, improved flavor and early production. Brazilian tomato breeding programs should consider not only the number of fruit but also fruit size because Brazilian consumers value fruit that are homogeneous, large and heavy. Our experiment was a randomized block design with three replicates of 32 tomato accessions from the Vegetable Germplasm Bank (Banco de Germoplasma de Hortaliças) at the Federal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lycopersicon esculentum; Genetic resources; Principal components; Repeatability coefficient; Determination coefficient.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100021

High frequency of the HRAS oncogene codon 12 mutation in Macedonian patients with urinary bladder cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Panov,Sasho; Roganovic-Zafirova,Danica; Stavric,George; Yashar,Genghis; Popov,Zivko.
Point mutations at codon 12 of the HRAS (v-Ha-ras Harvey rat sarcoma viral oncogene homolog) oncogene are one of the best defined and widely studied molecular genetic events in transitional cell carcinoma (TCC) of the urinary bladder. The aim of this study was to use the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of paraffin-embedded tissue-derived DNA to determine the frequency of the HRAS oncogene G -&gt;T codon 12 mutation in TCC patients being treated at the University Urology Clinic in Skopje, Republic of Macedonia. DNA isolated from paraffin-embedded tissue (PET) surgically removed TCC specimens of 62 (81.58%) out of 76 patients were successfully amplified, the remaining 14 (18.42%) showing compromised DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HRAS; Oncogene; Bladder cancer; PCR-RFLP.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100002

Identification of SNPs in RNA-seq data of two cultivars of Glycine max (soybean) differing in drought resistance
Provedor de dados: Genetics and Molecular Biology
Autores: Vidal,Ramon Oliveira; Nascimento,Leandro Costa do; Maurício Costa Mondego,Jorge; Amarante Guimarães Pereira,Gonçalo; Falsarella Carazzolle,Marcelo.
The legume Glycine max (soybean) plays an important economic role in the international commodities market, with a world production of almost 260 million tons for the 2009/2010 harvest. The increase in drought events in the last decade has caused production losses in recent harvests. This fact compels us to understand the drought tolerance mechanisms in soybean, taking into account its variability among commercial and developing cultivars. In order to identify single nucleotide polymorphisms (SNPs) in genes up-regulated during drought stress, we evaluated suppression subtractive libraries (SSH) from two contrasting cultivars upon water deprivation: sensitive (BR 16) and tolerant (Embrapa 48). A total of 2,222 soybean genes were up-regulated in both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single nucleotide polymorphisms; Deep sequencing; Drought resistance.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200014

The anticancer agent PB-100, selectively active on malignant cells, inhibits multiplication of sixteen malignant cell lines, even multidrug resistant
Provedor de dados: Genetics and Molecular Biology
Autores: Beljanski,Mirko.
The plant-derived anticancer agent PB-100 selectively destroys cancer cells, even when multidrug resistant; yet, it does not inhibit normal (non-malignant) cell multiplication. Testing of PB-100 on sixteen malignant cell lines, several multidrug resistant, as well as on five normal cell lines, confirmed our previous results. Flavopereirine and dihydroflavopereirine, the active principles of PB-100, were chemically synthesized and displayed the same selectivity for tumor cells as the purified plant extract, being active at even lower concentrations.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100005

Chromosome studies on Brazilian cerrado plants
Provedor de dados: Genetics and Molecular Biology
Autores: Forni-Martins,Eliana Regina; Martins,Fernando Roberto.
Cerrado is the Brazilian name for the neotropical savanna, which occurs mainly in Brazilian Central Plateau, composed of herbaceous-subshrubby and shrubby-arboreal floras, both of which are heliophilous, highly diverse and regionally differentiated. Considering species distribution and chromosome numbers, some authors have proposed that the herbaceous-subshrubby flora of the neotropical savanna is quite old, while the shrubby-arboreal flora is derived from forests, a hypothesis that implies higher chromosome numbers in the savanna than in the forest. If, however, chromosome numbers are similar in the cerrado and in forests, both could be similarly old, indicating that bi-directional flow of flora occurred in the past. This paper presents data on chromosome...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400040

Developmental and hyperthermia-induced expression of the heat shock proteins HSP60 and HSP70 in tissues of the housefly Musca domestica: an in vitro study
Provedor de dados: Genetics and Molecular Biology
Autores: Sharma,Sunita; Rohilla,Manoj Singh; Tiwari,Pramod Kumar.
The expression pattern of two major chaperones, the heat shock proteins (HSPs) HSP60 and HSP70 was studied in vitro in tissues of the housefly Musca domestica during larval and adult stages of development to identify their immunological relatives and understand their functional significance in normal cellular activities and during thermal stress. Fluorographs of labeled polypeptides and western blots demonstrated that both HSPs are expressed constitutively and heat-induced in all the larval and adult cell types examined. The pattern of whole tissue immunocytochemical staining using anti-HSP60 and anti-HSP70 antibodies corresponded well with the observations from western blots or fluorographs. In developing oocytes, both constitutive and heat inducible...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heat stress response; HSP60; HSP70; Musca domestica; Thermoprotection; Housefly.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100027

In memory of James F. Crow (1916-2012), a life dedicated to population genetics; with an updated list of his publications
Provedor de dados: Genetics and Molecular Biology
Autores: Otto,Paulo A.
Tipo: Info:eu-repo/semantics/other
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100028

Music genetics research: Association with musicality of a polymorphism in the AVPR1A gene
Provedor de dados: Genetics and Molecular Biology
Autores: Mariath,Luiza Monteavaro; Silva,Alexandre Mauat da; Kowalski,Thayne Woycinck; Gattino,Gustavo Schulz; Araujo,Gustavo Andrade de; Figueiredo,Felipe Grahl; Tagliani-Ribeiro,Alice; Roman,Tatiana; Vianna,Fernanda Sales Luiz; Schuler-Faccini,Lavínia; Schuch,Jaqueline Bohrer.
Abstract Musicality is defined as a natural tendency, sensibility, knowledge, or talent to create, perceive, and play music. Musical abilities involve a great range of social and cognitive behaviors, which are influenced by both environmental and genetic factors. Although a number of studies have yielded insights into music genetics research, genes and biological pathways related to these traits are not fully understood. Our hypothesis in the current study is that genes associated with different behaviors could also influence the musical phenotype. Our aim was to investigate whether polymorphisms in six genes (AVPR1A, SLC6A4, ITGB3, COMT, DRD2 and DRD4) related to social and cognitive traits are associated with musicality in a sample of children....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Musicality; Music; Vasopressin; AVPR1A.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300421

Investigator Argus X-12 study on the population of northern Croatia
Provedor de dados: Genetics and Molecular Biology
Autores: Crnjac,Josip; Ozretić,Petar; Merkaš,Siniša; Ratko,Martina; Lozančić,Mateja; Korolija,Marina; Popović,Maja; Mršić,Gordan.
Abstract X chromosome STR typing has emerged recently as a powerful tool, complementary to autosomal STR typing, in solving complex forensic and missing person cases. Investigator® Argus X-12 is a commercial product that allows co-amplification of 12 X chromosomal markers belonging to four linkage groups (LGs). In this study, we analyzed by capillary electrophoresis blood samples from 100 females and 102 males from a population of northern Croatia. Statistical analysis included calculation of allele and haplotype frequencies, as well as forensic parameters. The most informative marker for the northern Croatia population was DXS10135 with PIC=0.9211 and a total of 27 alleles. The least polymorphic marker was DXS8378 with 6 alleles. The proportion of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: X-STR; Forensic genetics; Investigator® Argus X-12; Population genetics; Northern Croatia.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100080

Cytogenetic characterization of F1, F2 and backcross hybrids of the Neotropical catfish species Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum (Pimelodidae, Siluriformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Prado,Fernanda Dotti do; Nunes,Tatiana Leite; Senhorini,José Augusto; Bortolozzi,Jehud; Foresti,Fausto; Porto-Foresti,Fábio.
The cytogenetic characteristics of Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum and their F1, F2 and backcross hybrids were assessed by using chromosome banding techniques. The diploid number of 56 chromosomes was constant in all species and lineages, with a karyotypic formula containing 20 metacentric, 12 submetacentric, 12 subtelocentric and 12 acrocentric chromosomes. Nucleolar organizer regions (NORs) were identified in two subtelocentric chromosomes in the parents and hybrids, with partial nucleolar dominance in F1 and F2 specimens. Heterochromatic blocks were detected in the terminal and centromeric regions of some chromosomes in all individuals. For parental and hybrid lineages, 18S ribosomal clusters corresponding to NORs and 5S...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Backcrossing; Cytogenetics; Interspecific hybrids; Post-F1 hybrids; Pseudoplatystoma.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100008

Polymorphism of the rDNA and tDNA loci in clinical isolates of Pseudomonas aeruginosa: a perspective for molecular epidemiology surveillance
Provedor de dados: Genetics and Molecular Biology
Autores: Spacov,Isabel Cristina Guerra; Silva,Suzileyde Alberto Marques da; Morais Júnior,Marcos Antônio de; Morais,Márcia Maria Camargo de.
The Gram-negative bacterium Pseudomonas aeruginosa has a wide environmental and ecological distribution. It is an opportunistic pathogen that acquires resistance to multiple antimicrobial agents and can infect plants, animals and humans. We used rDNA and tDNA PCR markers to characterize the bacterial diversity of P. aeruginosa strains isolated at a Brazilian teaching hospital (Oswaldo Cruz University Hospital, Recife, Brazil) between March 2003 and February 2004. Clonal groups of P. aeruginosa clinical isolates were identified from different patients in different hospital units using either rDNA or tDNA markers, or a combination of both in a duplex PCR. These PCR-typing methods together with drug-resistance profiles were used to trace the distribution of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic resistance; Molecular typing; Nosocomial infection; P. aeruginosa; RDNA-PCR; TDNA-PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400023

Dispersion of transposable elements and multigene families: Microstructural variation in Characidium (Characiformes: Crenuchidae) genomes
Provedor de dados: Genetics and Molecular Biology
Autores: Pucci,Marcela Baer; Nogaroto,Viviane; Moreira-Filho,Orlando; Vicari,Marcelo Ricardo.
Abstract Eukaryotic genomes consist of several repetitive DNAs, including dispersed DNA sequences that move between chromosome sites, tandem repeats of DNA sequences, and multigene families. In this study, repeated sequences isolated from the genome of Characidium gomesi were analyzed and mapped to chromosomes in Characidium zebra and specimens from two populations of C. gomesi. The sequences were transposable elements (TEs) named retroelement of Xiphophorus (Rex); multigene families of U2 small nuclear RNA (U2 snRNA); and histones H1, H3, and H4. Sequence analyses revealed that U2 snRNA contains a major portion corresponding to the Tx1-type non-LTR retrotransposon Keno, the preferential insertion sites of which are U2 snRNA sequences. All histone...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mobile DNA; Histones; Karyotype evolution; SnRNA; WZ/ZZ.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400585

Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream
Provedor de dados: Genetics and Molecular Biology
Autores: Sofia,Silvia H.; Galindo,Bruno A.; Paula,Francine M.; Sodré,Leda M.K.; Martinez,Cláudia B.R.
In this study, random amplified polymorphic DNA (RAPD) markers were applied to analyze the genetic diversity of samples of the Neotropical catfish Hypostomus ancistroides, collected from four sites (S1, S2, S3 and S4) along an urban stream in Southern Brazil. The 11 primers used in RAPD analysis amplified 147 loci, 76 (51.7%) of which were polymorphic. The proportions of polymorphic loci observed in the four samples were: 29.93% (S1), 31.97% (S2), 23.81% (S3) and 38.77% (S4). The average heterozygosity within sampling localities ranged from 0.1230 to 0.1526 and unbiased genetic distances ranged from 0.0253 to 0.0445. AMOVA partitioned 90.85% of the total variation within samples and 9.15% among samples. Excepting for the sample pair S1-S2 (phiST = 0.02784;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loricariid fish; Genetic diversity; RAPD; Siluriformes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200027

A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing
Provedor de dados: Genetics and Molecular Biology
Autores: Xiao,Junhua; Xin,Xiujuan; Luan,Xiaohui; Dongzhi,Wei; Shengli,Yang.
We describe a modified single nucleotide polymorphism (SNP) typing method based on the restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR). This is a simple, economical method without the need for special equipment. For most SNP loci, a common restriction endonuclease (Hind III, EcoR I or BamH I) recognizing site (RER) can be introduced into one allelic form, but not the other by two rounds of mismatched PCR. The flanking regions can be changed by as many as five bases after PCR amplification with specially designed mismatching primers so the genotypes can be distinguished after digestion of the PCR products with corresponding endonucleases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SNP genotyping; PCR-RFLP; RER site.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300028

Cytogenetic studies in fishes of the genera Hassar, Platydoras and Opsodoras (Doradidae, Siluriformes) from Jarí and Xingú Rivers, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Milhomem,Susana Suely Rodrigues; Souza,Augusto Cesar Paes de; Nascimento,Aline Lira do; Carvalho Jr.,Jaime Ribeiro; Feldberg,Eliana; Pieczarka,Julio Cesar; Nagamachi,Cleusa Yoshiko.
We studied the karyotypes of Hassar cf. orestis and an undescribed Hassar species from the Jarí River and Opsodoras ternetzi, H. orestis and Platydoras cf. costatus from the Xingú River, all with 2n = 58. Constitutive heterochromatin is located in the centromere in most metacentric pairs; in some chromosomes this banding is not present, or it is located on the whole chromosome arm or in the distal regions. The NOR is located on a single biarmed pair at a distal region of the short arm in H. cf. orestis, H. orestis and P. cf. costatus at a distal region of the long arm in O. ternetzi and at a proximal region of the long arm in the Hassar species. In all species (except for Hassar sp.) the CMA3 analysis revealed a rich G-C region coincident with the NOR....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomes; Amazon; Biodiversity; Siluriformes; Banding; Fluorochromes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200017

Restriction fragment analysis of the ribosomal DNA of Paratelmatobius and Scythrophrys species (Anura, Leptodactylidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Lourenço,Luciana B.; Garcia,Paulo C.A.; Recco-Pimentel,Shirlei M.
Physical maps of the ribosomal RNA gene 28S of species belonging to the genera Paratelmatobius and Scythrophrys were constructed, using five restriction endonucleases. The restriction sites for Bam HI, Bgl II, Bst EII, and Eco RI had similar positions in all species, although there were interspecific differences in the size of the restriction fragments obtained. An additional Pvu II site was found in Scythrophrys specimens from Piraquara (State of Paraná, Brazil) and from São Bento do Sul (State of Santa Catarina, Brazil), but not in the Scythrophrys specimens from Rancho Queimado (State of Santa Catarina, Brazil). This finding is in agreement with the hypothesis regarding the existence of two species in the genus Scythrophrys. On the other hand, the extra...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ribosomal DNA; 28S rDNA gene; Restriction sites; Physical map; Paratelmatobius; Scythrophrys; Leptodactylidae; Anura.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200006

Molecular cloning and functional analysis of the fatty acid-binding protein (Sp-FABP) gene in the mud crab (Scylla paramamosain)
Provedor de dados: Genetics and Molecular Biology
Autores: Zeng,Xianglan; Ye,Haihui; Yang,Ya'nan; Wang,Guizhong; Huang,Huiyang.
Intracellular fatty acid-binding proteins (FABPs) are multifunctional cytosolic lipid-binding proteins found in vertebrates and invertebrates. In this work, we used RACE to obtain a full-length cDNA of Sp-FABP from the mud crab Scylla paramamosain. The open reading frame of the full length cDNA (886 bp) encoded a 136 amino acid polypeptide that showed high homology with related genes from other species. Real-time quantitative PCR identified variable levels of Sp-FABP transcripts in epidermis, eyestalk, gill, heart, hemocytes, hepatopancreas, muscle, ovary, stomach and thoracic ganglia. In ovaries, Sp-FABP expression increased gradually from stage I to stage IV of development and decreased in stage V. Sp-FABP transcripts in the hepatopancreas and hemocytes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatty acid-binding protein; Immunity; Ovary development; Real-time quantitative PCR; Scylla paramamosain.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100021

Research of the origin of a particular Tunisian group using a physical marker and Alu insertion polymorphisms
Provedor de dados: Genetics and Molecular Biology
Autores: Moncer,Wifak El; Bahri,Raoudha; Esteban,Esther; Abdenni-Guenounou,Bouthaina; Moral,Pedro; Chibani,Jemni Ben; Chaabani,Hassen.
The aim of this study was to show how, in some particular circumstances, a physical marker can be used along with molecular markers in the research of an ancient people movement. A set of five Alu insertions was analysed in 42 subjects from a particular Tunisian group (El Hamma) that has, unlike most of the Tunisian population, a very dark skin, similar to that of sub-Saharans, and in 114 Tunisian subjects (Gabes sample) from the same governorate, but outside the group. Our results showed that the El Hamma group is genetically midway between sub-Saharan populations and North Africans, whereas the Gabes sample is clustered among North Africans. In addition, The A25 Alu insertion, considered characteristic to sub-Saharan Africans, was present in the El Hamma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tunisian population; El Hamma group; Skin color; Alu insertion polymorphisms; People movement history.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300002

Identification of novel and conserved microRNAs in Coffea canephora and Coffea arabica
Provedor de dados: Genetics and Molecular Biology
Autores: Loss-Morais,Guilherme; Ferreira,Daniela C.R.; Margis,Rogério; Alves-Ferreira,Márcio; Corrêa,Régis L.
As microRNAs (miRNAs) are important regulators of many biological processes, a series of small RNAomes from plants have been produced in the last decade. However, miRNA data from several groups of plants are still lacking, including some economically important crops. Here microRNAs from Coffea canephora leaves were profiled and 58 unique sequences belonging to 33 families were found, including two novel microRNAs that have never been described before in plants. Some of the microRNA sequences were also identified in Coffea arabica that, together with C. canephora, correspond to the two major sources of coffee production in the world. The targets of almost all miRNAs were also predicted on coffee expressed sequences. This is the first report of novel miRNAs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; MicroRNA profiling; Illumina sequencing.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500010

Differential gene expression and mitotic cell analysis of the drought tolerant soybean (Glycine max L. Merrill Fabales, Fabaceae) cultivar MG/BR46 (Conquista) under two water deficit induction systems
Provedor de dados: Genetics and Molecular Biology
Autores: Martins,Polyana K.; Jordão,Berenice Q.; Yamanaka,Naoki; Farias,José R.B.; Beneventi,Magda A.; Binneck,Eliseu; Fuganti,Renata; Stolf,Renata; Nepomuceno,Alexandre L.
Drought cause serious yield losses in soybean (Glycine max), roots being the first plant organ to detect the water-stress signals triggering defense mechanisms. We used two drought induction systems to identify genes differentially expressed in the roots of the drought-tolerant soybean cultivar MG/BR46 (Conquista) and characterize their expression levels during water deficit. Soybean plants grown in nutrient solution hydroponically and in sand-pots were submitted to water stress and gene expression analysis was conducted using the differential display (DD) and real time polymerase chain reaction (PCR) techniques. Three differentially expressed mRNA transcripts showed homology to the Antirrhinum majus basic helix-loop-helix transcription factor bHLH, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell division; Differential display; Gene expression; Real time PCR; Soybean; Water stress.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300019

The association of CAPN1 316 marker genotypes with growth and meat quality traits of steers finished on pasture
Provedor de dados: Genetics and Molecular Biology
Autores: Miquel,María C.; Villarreal,Edgardo; Mezzadra,Carlos; Melucci,Lilia; Soria,Liliana; Corva,Pablo; Schor,Alejandro.
The objective of this paper was to determine the association of a SNP in the µ-calpain gene at position 316 with growth and quality of meat traits of steers grown on pasture. Fifty-nine Brangus and 20 Angus steers were genotyped for CAPN1 316. Warner Bratzler shear force was measured in l. lumborum samples after a 7-day aging period. A multivariate analysis of variance was performed, including shear force (WBSF), final weight (FW), average daily gain (ADG), backfat thickness (BFT), average monthly fat thickness gain (AMFTG), rib-eye area (REA), and beef rib-eye depth (RED) as dependent variables. The CAPN1 316 genotype was statistically significant. Univariate analyses were done with these variables. The marker genotype was statistically significant (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Tenderness; Growth; Molecular marker; Multivariate analysis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300011

Genetic characterization of 18 novel microsatellite loci in northern pike (Esox lucius L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,Jun; Wang,Chenghui; Qian,Long; Ma,Yuqing; Yang,Xinxin; Jeney,Zsigmond; Li,Sifa.
The northern pike (Esox lucius L.), an important predatory freshwater species, is undergoing significant population decline. In this study, 18 novel polymorphic microsatellite loci were isolated and used for assessing genetic variation in the Chinese Ulungur and Hungarian Balaton populations of the species. The number of alleles ranged from 2 to 13, observed heterozygosity from 0.154 to 0.920 and expected heterozygosity from 0.145 to 0.921, thereby indicating the specific usefulness of these suites of markers for investigating genetic variability.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Northern pike; Microsatellite loci; Genetic variability.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100029

The parametric restrictions of the Griffing diallel analysis model: combining ability analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Viana,José Marcelo Soriano.
It was studied the parametric restrictions of the diallel analysis model of Griffing, method 2 (parents and F1 generations) and model 1 (fixed), in order to address the questions: i) does the statistical model need to be restricted? ii) do the restrictions satisfy the genetic parameter values? and iii) do they make the analysis and interpretation easier? Objectively, these questions can be answered as: i) yes, ii) not all of them, and iii) the analysis is easier, but the interpretation is the same as in the model with restrictions that satisfy the parameter values. The main conclusions were that: the statistical models for combining ability analysis are necessarily restricted; in the Griffing model (method 2, model 1), the restrictions relative to the...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400029

Evaluation of noise reduction techniques in the splice junction recognition problem
Provedor de dados: Genetics and Molecular Biology
Autores: Lorena,Ana C.; Carvalho,André C. P. L. F. de.
The Human Genome Project has generated a large amount of sequence data. A number of works are currently concerned with analyzing these data. One of the analyses carried out is the identification of genes' structures on the sequences obtained. As such, one can search for particular signals associated with gene expression. Splice junctions represent a type of signal present on eukaryote genes. Many studies have applied Machine Learning techniques in the recognition of such regions. However, most of the genetic databases are characterized by the presence of noisy data, which can affect the performance of the learning techniques. This paper evaluates the effectiveness of five data pre-processing algorithms in the elimination of noisy instances from two splice...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pre-processing; Machine learning; Splice junction recognition.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400031

Karyotypic description of four species of Trigona (Jurine, 1807) (Hymenoptera, Apidae, Meliponini) from the State of Mato Grosso, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Kleber França; Brito,Rute Magalhães; Miyazawa,Carlos Suetoshi.
The genus Trigona contains at least 31 species, but there have been few cytogenetic studies of this group. In this work, four species of Trigona (T. branneri, T. chanchamayoensis, T. hyalinata, and T. recursa) from the municipality of Cuiabá, in the State of Mato Grosso, Brazil, were studied. In all of the species, the females had 2n = 34 chromosomes and the males had n = 17. The C-banding patterns showed that the karyotypes of these species consisted mainly of acrocentric and pseudoacrocentric chromosomes. These cytogenetic findings should useful in future phylogenetic studies of this group.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Karyotype; Meliponini; Stingless bees; Trigona.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200010

Karyotypic characterization of Hydromedusa tectifera (Testudines, Pleurodira) from the upper Iguaçu River in the Brazilian state of Paraná
Provedor de dados: Genetics and Molecular Biology
Autores: Noleto,Rafael Bueno; Kantek,Daniel Luis Zanella; Swarça,Ana Cláudia; Dias,Ana Lúcia; Fenocchio,Alberto Sergio; Cestari,Marta Margarete.
We present the karyotypic characterization of 26 specimens of the side-necked turtle Hydromedusa tectifera collected in the upper Iguaçu River, Paraná state, Brazil. The turtles were cytogenetically analyzed using Giemsa staining and other banding techniques (C, G, Ag-NOR and CMA3) as well as fluorescence in situ hybridization (FISH) with a rDNA 18S probe. All the specimens showed a diploid number of 58 composed of 22 macro and 36 microchromosomes. The Ag-NOR, CMA3 and FISH techniques permitted the identification and characterization of the chromosome pairs bearing nucleolus organizer regions (NORs), while G-banding facilitated a better recognition and pairing of macrochromosomes. These data agree with some information available in the literature and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomes; FISH; G-banding; Pleurodira; Turtle.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200011

Association between G316A growth hormone polymorphism and economic traits in pigs
Provedor de dados: Genetics and Molecular Biology
Autores: Faria,Danielle Assis de; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Pires,Aldrin Vieira; Paiva,Samuel Rezende; Sollero,Bruna Pena; Wenceslau,Amauri Arias.
The association between G316A growth hormone polymorphism and quantitative traits was investigated in an F2 population of pigs. Association analyses were performed using a statistical model that included genotype, sex, batch and sex by genotype interaction as fixed effects and sire as random effect. The polymorphism was associated with the number of right teats (p = 0.03), heart weight (p = 0.04), lung weight (p = 0.05), carcass length determined by the Brazilian carcass classification method (p = 0.04), picnic shoulder weight (p = 0.07), jowl weight (p = 0.01), pH 24 h after slaughtering (p = 0.03) and drip loss (p = 0.01). Interaction between genotype and sex was observed for six performance traits. The additive effect was significant (p &lt; 0.10) for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candidate genes; Divergent cross; Molecular marker; Sex x genotype interaction; Swine.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400010

A note on the population genetic consequences of delayed larval development in insects
Provedor de dados: Genetics and Molecular Biology
Autores: Salles,Marcos Mattoso de; Otto,Paulo A.
Observations by Dobzhansky's group in the 1940s suggesting that the presence of recessive genotypes could account for lower larval developmental rates in Drosophila melanogaster were not confirmed at the time and all subsequent investigations on this subject focused on the analysis of ecological models based on competition among pre-adult individuals. However, a paper published in this journal in 1991 eventually confirmed the finding made by Dobzhansky and his co-workers. In this report, we provide a theoretical analysis of the population genetic effects of a delay in the rate of larval development produced by such a genetic mechanism.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Difference and differential equations; Drosophila; Dynamic systems; Larval development; Population genetics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300019

Chromosomal locations of the maize (Zea mays L.) HtP and rt genes that confer resistance to Exserohilum turcicum
Provedor de dados: Genetics and Molecular Biology
Autores: Ogliari,Juliana Bernardi; Guimarães,Marco Antônio; Camargo,Luis Eduardo Aranha.
We used 125 microsatellite markers to genotype the maize (Zea mays L.) near isogenic lines (NIL) L30HtPHtPRtRt and L30htphtpRtRt and the L40htphtprtrt line which contrast regarding the presence of the recently described dominant HtP and the recessive rt genes that confer resistance to Exserohilum turcicum. Five microsatellite markers revealed polymorphisms between the NIL and were considered candidate linked markers for the HtP resistance gene. Linkage was confirmed by bulked segregant sample (BSS) analysis of 32 susceptible and 34 resistant plants from a BC1F1 population derived from the cross (L30HtPHtPRtRt x L40htphtprtrt) x L40htphtprtrt. The bnlg198 and dupssr25 markers, both located on maize chromosome 2L (bin 2.08), were polymorphic between bulks....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bulked segregant sample analysis; Microsatellite markers; Maize; Near-isogenic lines; Northern leaf blight.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400021

Genetic analysis of adult-plant resistance to leaf rust in a double haploid wheat (Triticum aestivum L. em Thell) population
Provedor de dados: Genetics and Molecular Biology
Autores: Brammer,Sandra Patussi; Fernandes,Maria Irene Baggio de Moraes; Barcellos,Amarilis Labes; Milach,Sandra Cristina Kothe.
A genetic analysis of adult plant resistance to leaf rust (Puccinia triticina) was performed in in vitro obtained double haploid progenies from a cross between the Brazilian wheat cultivar Trigo BR 35, which, under the high inoculum pressure of the southern region, has been resistant to leaf rust for more than 12 years, and the susceptible cultivar IAC 13-Lorena. Haplodiploidization via in vitro gimnogenesis was done by somatic elimination of the pollen donor genome after maize pollination of the F1 plants. The advantages and usefulness of double haploids (DH) for genetic analysis of complex inherited traits like durable adult-plant resistance to wheat leaf rust were evident: it was possible to analyze inheritance patterns in this cross by using only the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Puccinia triticina; Resistant genes; Haplodiploidization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300020

-866G/A and Ins/Del polymorphisms in the UCP2 gene and diabetic kidney disease: case-control study and meta-analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Dieter,Cristine; Assmann,Taís Silveira; Lemos,Natália Emerim; Massignam,Eloísa Toscan; Souza,Bianca Marmontel de; Bauer,Andrea Carla; Crispim,Daisy.
Abstract Uncoupling protein 2 (UCP2) decreases reactive oxygen species (ROS). ROS overproduction is a key contributor to the pathogenesis of diabetic kidney disease (DKD). Thus, UCP2 polymorphisms are candidate risk factors for DKD; however, their associations with this complication are still inconclusive. Here, we describe a case-control study and a meta-analysis conducted to investigate the association between UCP2 -866G/A and Ins/Del polymorphisms and DKD. The case-control study comprised 385 patients with type 1 diabetes mellitus (T1DM): 223 patients without DKD and 162 with DKD. UCP2 -866G/A (rs659366) and Ins/Del polymorphisms were genotyped by real-time PCR and conventional PCR, respectively. For the meta-analysis, a literature search was conducted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: UCP2; Polymorphisms; Diabetic kidney disease.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400101

Method optimization for proteomic analysis of soybean leaf: improvements in identification of new and low-abundance proteins
Provedor de dados: Genetics and Molecular Biology
Autores: Mesquita,Rosilene Oliveira; Soares,Eduardo de Almeida; Barros,Everaldo Gonçalves de; Loureiro,Marcelo Ehlers.
The most critical step in any proteomic study is protein extraction and sample preparation. Better solubilization increases the separation and resolution of gels, allowing identification of a higher number of proteins and more accurate quantitation of differences in gene expression. Despite the existence of published results for the optimization of proteomic analyses of soybean seeds, no comparable data are available for proteomic studies of soybean leaf tissue. In this work we have tested the effects of modification of a TCA-acetone method on the resolution of 2-DE gels of leaves and roots of soybean. Better focusing was obtained when both mercaptoethanol and dithiothreitol were used in the extraction buffer simultaneously. Increasing the number of washes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proteomics; Glycine max; 2-DE; Protein extraction; Leaf proteome.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200017

An effective homologous cloning method for isolating novel miR172s from Phalaenopsis hybrida
Provedor de dados: Genetics and Molecular Biology
Autores: Han,Ying Ying; Yan,Qin Hua; Ming,Feng.
MiR172 is an important microRNA that regulates floral development in various plants and downregulates AP2 family members to relieve the stress on floral determinacy, leading to phase transition from vegetative to reproductive growth. In this work, PCR with primers designed based on the rice miR172 sequence was used to isolate two miR172-like transcripts from Phalaenopsis hybrida (PhmiR172-1 and PhmiR172-2) that were very similar to Oryza miR172d and Arabidopsis miR172b. RT-PCR indicated that the levels of these two transcripts were negatively correlated with the level of the Phalaenopsis AP2 (PhAP2) gene in stem, root, pedicel and sepal, and that both were co-expressed with PhAP2 in young buds. Overproduction of PhmiR172-2 in Arabidopsis led to early...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homologous cloning; MiR172; MiRNA; Phalaenopsis; Sequence conservation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300014

Whole-genome duplication and hemoglobin differentiation traits between allopatric populations of Brazilian Odontophrynus americanus species complex (Amphibia, Anura)
Provedor de dados: Genetics and Molecular Biology
Autores: Cianciarullo,Aurora M.; Bonini-Domingos,Claudia R.; Vizotto,Luiz D.; Kobashi,Leonardo S.; Beçak,Maria-Luiza; Beçak,Willy.
Abstract Two allopatric populations of Brazilian diploid and tetraploid Odontophrynus americanus species complex, both from São Paulo state, had their blood hemoglobin biochemically analyzed. In addition, these specimens were cytogenetically characterized. Biochemical characterization of hemoglobin expression showed a distinct banding pattern between the allopatric specimens. Besides this, two distinct phenotypes, not linked to ploidy, sex, or age, were observed in adult animals of both populations. Phenotype A exhibits dark-colored body with small papillae, ogival-shaped jaw with reduced interpupillary distance and shorter hind limbs. Phenotype B shows yellowish-colored body with larger papillae, arch-shaped jaw with broader interpupillary distance and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anura; Cryptic species; Hemoglobin differentiation; Polyploidy; Whole-genome duplication.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300436

Evidence of apomixis in cassava  (Manihot esculenta Crantz)
Provedor de dados: Genetics and Molecular Biology
Autores: Nassar,Nagib M.A.; Vieira,Marco André R.; Vieira,Clibas; Gratapaglia,Dario.
Apomixis maintains heterosis and avoids transmission of systemic pathogens which accompany vegetative propagation of cassava. An embryonic study of cleared ovules of two cassava clones in toto showed them to be of aposporic nature. Cytogenetic analysis of the two clones revealed an aneuploid structure (2n + 1) in apomictic individuals, whereas it was 2n in the sexually reproduced plants.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400020

RAPD markers indicate the occurrence of structured populations in a migratory freshwater fish species
Provedor de dados: Genetics and Molecular Biology
Autores: Hatanaka,Terumi; Galetti Jr.,Pedro Manoel.
Many factors have contributed to the destruction of fish habitats. Hydroelectric dams, water pollution and other environmental changes have resulted in the eradication of natural stocks. The aim of this study was to detect the genetic variation in Prochilodus marggravii from three collection sites in the area of influence of the Três Marias dam (MG) on the São Francisco river (Brazil), using the RAPD technique. The results obtained revealed that the fish in the downstream region nearest the dam have a higher similarity coefficient than those from the other sampling sites that may be related to differences in environmental characteristics in these regions. Additionaly, significant differences in the band frequencies were observed from one collection site to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prochilodus; RAPD; Genetic structure; Conservation genetic; Fish.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100004

GJB2 c.235delC variant associated with autosomal recessive nonsyndromic hearing loss and auditory neuropathy spectrum disorder
Provedor de dados: Genetics and Molecular Biology
Autores: Xia,Hong; Huang,Xiangjun; Xu,Hongbo; Zhou,Yong-an; Gong,Lina; Yang,Zhijian; Lv,Jingyan; Deng,Hao.
Abstract Autosomal recessive nonsyndromic hearing loss (ARNSHL) is a genetically heterogeneous neurosensory disorder, usually characterized by congenital or prelingual hearing loss. We report a Han Chinese male, born to consanguineous parents, presenting with nonsyndromic sensorineural hearing loss, whose clinical phenotype was also consistent with auditory neuropathy spectrum disorder (ANSD). After exome sequencing, a gap junction protein beta 2 gene (GJB2) c.235delC variant in the homozygous state was detected in the patient. Both parents were heterozygous for this variant, as documented by Sanger sequencing. The known pathogenic GJB2 c.235delC variant was not detected in 200 healthy controls. It is predicted to be a disease-causing alteration by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Auditory neuropathy spectrum disorder; Exome sequencing; Hearing loss; GJB2 gene; GJB2 c.235delC variant.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100048

Evidence of ectopic recombination and a repeat-induced point (RIP) mutation in the genome of Sclerotinia sclerotiorum, the agent responsible for white mold
Provedor de dados: Genetics and Molecular Biology
Autores: Goldfarb,Míriam; Santana,Mateus Ferreira; Salomão,Tânia Maria Fernandes; Queiroz,Marisa Vieira de; Barros,Everaldo Gonçalves de.
Abstract Two retrotransposons from the superfamilies Copia and Gypsy named as Copia-LTR_SS and Gypsy-LTR_SS, respectively, were identified in the genomic bank of Sclerotinia sclerotiorum. These transposable elements (TEs) contained direct and preserved long terminal repeats (LTR). Domains related to codified regions for gag protein, integrase, reverse transcriptase and RNAse H were identified in Copia-LTR_SS, whereas in Gypsy-LTR_SS only domains for gag, reverse transcriptase and RNAse H were found. The abundance of identified LTR-Solo suggested possible genetic recombination events in the S. sclerotiorum genome. Furthermore, alignment of the sequences for LTR elements from each superfamily suggested the presence of a RIP (repeat-induced point mutation)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Phytopathogens; Retrotransposons; Transposable elements.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300426

Genetic structure of drone congregation areas of Africanized honeybees in southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Collet,Thais; Cristino,Alexandre Santos; Quiroga,Carlos Fernando Prada; Soares,Ademilson Espencer Egea; Del Lama,Marco Antônio.
As yet, certain aspects of the Africanization process are not well understood, for example, the reproductive behavior of African and European honeybees and how the first Africanized swarms were formed and spread. Drone congregation areas (DCAs) are the ideal place to study honeybee reproduction under natural conditions since hundreds of drones from various colonies gather together in the same geographical area for mating. In the present study, we assessed the genetic structure of seven drone congregations and four commercial European-derived and Africanized apiaries in southern Brazil, employing seven microsatellite loci for this purpose. We also estimated the number of mother-colonies that drones of a specific DCA originated from. Pairwise comparison...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drone congregation area; Africanization; Honeybee reproduction; Population genetics.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400030

Evaluation of genotoxicity through micronuclei test in workers of car and battery repair garages
Provedor de dados: Genetics and Molecular Biology
Autores: Martino-Roth,M.G.; Viégas,J.; Amaral,M.; Oliveira,L.; Ferreira,F.L.S.; Erdtmann,B.
In this study, the micronuclei test (MNT) was applied in exfoliated cells of buccal mucosa, in order to evaluate the genotoxic risk associated with occupational exposure of mechanics, storage battery renovation workers, and car painters. For each individual, 3000 exfoliated buccal cells were analyzed. There was a significantly higher frequency of micronucleated cells (MNC) in the exposed workers than in controls. Smoking and drinking habits, age, and working time did not represent significant factors in terms of increasing the production of micronuclei (MN), when the control and the exposed groups were compared. These results allowed to conclude that the studied individuals belong to a risk group and should periodically undergo biological monitoring and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronucleus test; Occupational exposure.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400021

Glutathione S-transferase mu 1 (GSTM1) and theta 1 (GSTT1) genetic polymorphisms and atopic asthma in children from Southeastern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Carmen Silvia Passos; Néri,Iramaia Angélica; Lourenço,Gustavo Jacob; Faria,Isabel Cristina Jacinto; Ribeiro,José Dirceu; Bertuzzo,Carmen Silvia.
Xenobiotics can trigger degranulation of eosinophils and mast cells. In this process, the cells release several substances leading to bronchial hyperactivity, the main feature of atopic asthma (AA). GSTM1 and GSTT1 genes encode enzymes involved in the inactivation of these compounds. Both genes are polymorphic in humans and have a null variant genotype in which both the gene and corresponding enzyme are absent. An increased risk for disease in individuals with the null GST genotypes is therefore, but this issue is controversial. The aim of this study was to investigate the influence of the GSTM1 and GSTT1 genotypes on the occurrence of AA, as well as on its clinical manifestations. Genomic DNA from 86 patients and 258 controls was analyzed by polymerase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atopic asthma; Pathogenesis; GSTM1 gene; GSTT1 gene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300007

Osteogenesis imperfecta in Brazilian patients
Provedor de dados: Genetics and Molecular Biology
Autores: Trancozo,Maira; Moraes,Marcos V.D.; Silva,Dalila A.; Soares,Jéssica A.M.; Barbirato,Clara; Almeida,Márcio G.; Santos,Lígia R.; Rebouças,Maria R. G. O.; Akel Jr,Akel N.; Sipolatti,Valentim; Nunes,Vanda R. R.; Errera,Flavia I. V.; Aguena,Meire; Passos-Bueno,Maria R.; Paula,Flavia de.
Abstract Osteogenesis Imperfecta (OI) is a heterogeneous genetic disorder characterized by bone fragility and fracture. Mutations in 20 distinct genes can cause OI, and therefore, the genetic diagnosis of OI is frequently difficult to obtain because of the great number of genes that can be related with this disease. Studies that report the most frequently mutated genes in OI patients can help to improve molecular strategies for diagnosis of the disease. In order to characterize the mutation profile of OI in Brazilian patients, we analyzed 30 unrelated patients through SSCP screening, NGS gene panel, and/or Sanger sequencing for the 11 most frequently mutated genes in the database of mutations, including COL1A1, COL1A2, P3H1, CRTAP, PPIB, SERPINH1,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NGS gene panel; COL1A1/COL1A2 genes; FKBP10 gene; P3H1 gene; IFITM5 gene.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300344

Quantitative genetics theory for non-inbred populations in linkage disequilibrium
Provedor de dados: Genetics and Molecular Biology
Autores: Viana,José Marcelo Soriano.
Although linkage disequilibrium, epistasis and inbreeding are common phenomena in genetic systems that control quantitative traits, theory development and analysis are very complex, especially when they are considered together. The objective of this study is to offer additional quantitative genetics theory to define and analyze, in relation to non-inbred cross pollinating populations, components of genotypic variance, heritabilities and predicted gains, assuming linkage disequilibrium and absence of epistasis. The genotypic variance and its components, additive and due to dominance genetic variances, are invariant over the generations only in regard to completely linked genes and to those in equilibrium. When the population is structured in half-sib...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Components of genotypic variance; Heritabilities; Half-sib families; Full-sib families.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400021

Erratum: 29(2) p 408-412
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/article
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400031

Chromosome studies in Orchidaceae from Argentina
Provedor de dados: Genetics and Molecular Biology
Autores: Daviña,Julio Rubén; Grabiele,Mauro; Cerutti,Juan Carlos; Hojsgaard,Diego Hernán; Almada,Rubén Dario; Insaurralde,Irma Stella; Honfi,Ana Isabel.
The center of diversity of Argentinean orchids is in the northeast region of the country. Chromosome numbers and karyotype features of 43 species belonging to 28 genera are presented here. Five chromosome records are the first ones at the genus level; these taxa are Aspidogyne kuckzinskii (2n = 42), Eurystyles actinosophila (2n = 56), Skeptrostachys paraguayensis (2n = 46), Stigmatosema polyaden (2n = 40) and Zygostates alleniana (2n = 54). In addition, a chromosome number is presented for the first time for 15 species: Corymborkis flava (2n = 56), Cyclopogon callophyllus (2n = 28), C. oliganthus (2n = 64), Cyrtopodium hatschbachii (2n = 46), C. palmifrons (2n = 46), Galeandra beyrichii (2n = 54), Habenaria bractescens (2n = 44), Oncidium edwallii (2n =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome number; Karyotype features; Polyploidy; Karyology; Orchids; South America.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400024

Molecular cytogenetic characterization of parental genomes in the partial amphidiploid Triticum aestivum x Thinopyrum ponticum
Provedor de dados: Genetics and Molecular Biology
Autores: Brasileiro-Vidal,Ana Christina; Cuadrado,Angeles; Brammer,Sandra P.; Benko-Iseppon,Ana Maria; Guerra,Marcelo.
The wheat line PF 839197 and six hybrid derivatives from a cross between PF 839197 and Thinopyrum ponticum were cytologically characterized by fluorescent in situ hybridization (FISH). Probes for the 5S and 45S rDNA genes (pTa794 and pTa71, respectively), a highly repetitive rye sequence (pSc119.2), the synthetic oligonucleotide (AAG)5, and total genomic DNA from Th. ponticum and rye were used. In the wheat line, a 1RS.1BL translocation was revealed by the labeling patterns produced with pSc119.2 and (AAG)5, and confirmed by genomic in situ hybridization (GISH) using rye genomic DNA as a probe. Analyses of partial amphiploids confirmed previous results indicating mitotic instability, with a tendency to stabilize at 2n = 42 or 56. GISH with Th. ponticum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; Thinopyrum ponticum; Rye; Hybrid derivatives; GISH.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200022

Sex determination in annual fishes: searching for the master sex-determining gene in Austrolebias charrua (Cyprinodontiformes, Rivulidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Arezo,María José; Papa,Nicolás; Guttierrez,Verónica; García,Graciela; Berois,Nibia.
Evolution of sex determination and differentiation in fishes involves a broad range of sex strategies (hermaphroditism, gonochorism, unisexuality, environmental and genetic sex determination). Annual fishes inhabit temporary ponds that dry out during the dry season when adults die. The embryos exhibit an atypical developmental pattern and remain buried in the bottom mud until the next rainy season. To elucidate genomic factors involved in the sex determination in annual fish, we explored the presence of a candidate sex-specific gene related to the cascade network in Austrolebias charrua. All phylogenetic analyses showed a high posterior probability of occurrence for a clade integrated by nuclear sequences (aprox. 900 bp) from both adults (male and female),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Annual fish; Development; Sex determination; Doublesex gene related sequence.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300008

Clinical and biochemical findings in 7 patients with X-linked adrenoleukodystrophy treated with Lorenzo's Oil
Provedor de dados: Genetics and Molecular Biology
Autores: Vargas,Carmen R.; Barschak,Alethéa G.; Coelho,Daniella M.; Furlanetto,Vivian; Souza,Carolina F.M. de; Karam,Simone M.; Jardim,Laura; Wajner,Moacir; Giugliani,Roberto.
X-Linked adrenoleukodystrophy (X-ALD) is a hereditary disorder of the peroxisomal metabolism biochemically characterized by the accumulation of very long chain fatty acids (VLCFA) in tissues and biological fluids. The major accumulated acids are hexacosanoic acid (C26:0) and tetracosanoic acid (C24:0). The disorder is characterized clinically by central and peripheral demyelination and adrenal insufficiency closely related to the accumulation of fatty acids. The incidence of X-ALD is estimated to be 1:25,000 males. At least six phenotypes can be distinguished. The most common phenotypes are childhood cerebral ALD and adrenomyeloneuropathy (AMN). The recommended therapy consists of the use of the glyceroltrioleate/glyceroltrierucate (GTO/GTE) mixture, known...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400001

Calibration curves by 60Co with low dose rate are different in terms of dose estimation – a comparative study
Provedor de dados: Genetics and Molecular Biology
Autores: Mendes,Mariana Esposito; Mendonça,Julyanne Conceição Goes de; Hwang,Suy; Giorgio,Marina Di; Lima,Fabiana Farias de; Santos,Neide.
Abstract Biological dosimetry aims to estimate individual absorbed doses due ionizing radiation exposure. The dicentric chromosomes are considered the most specific biomarker for dose estimation. This study aimed to compare calibration curves for linear low energy transfer (LET) radiation built from low dose rates and whether they vary in terms of dose estimation. For that we did a search in the literature of all calibration curves produced with low dose rates and we simulated the dose estimation from pre-established dicentric’s frequencies. The information on methodologies and cytogenetic results of each study were analyzed. As expected dose rate influence β coefficients, especially at higher doses. However, we have seen that some doses were not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dicentrics; Gamma radiation; Biological dosimetry; Intercomparisons.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100108

DNA damage protective effect of honey-sweetened cashew apple nectar in Drosophila melanogaster
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Robson Alves da; Dihl,Rafael Rodrigues; Dias,Lucas Pinheiro; Costa,Maiane Papke; Abreu,Bianca Regina Ribas de; Cunha,Kênya Silva; Lehmann,Mauricio.
Abstract Fruits and derivatives, such as juices, are complex mixtures of chemicals, some of which may have mutagenic and/or carcinogenic potential, while others may have antimutagenic and/or anticancer activities. The modulating effects of honey-sweetened cashew apple nectar (HSCAN), on somatic mutation and recombination induced by ethyl methanesulfonate (EMS) and mitomycin C (MMC) were evaluated with the wing spot test in Drosophila melanogaster using co- and post-treatment protocols. Additionally, the antimutagenic activity of two HSCAN components, cashew apple pulp and honey, in MMC-induced DNA damage was also investigated. HSCAN reduced the mutagenic activity of both EMS and MMC in the co-treatment protocol, but had a co-mutagenic effect when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimutagenicity; Cashew apple; Drosophila melanogaster; Honey; Somatic cells.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300431

Erratum
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400021

Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Aragão,F.J.L.; Barros,L.M.G.; Sousa,M.V. de; Grossi de Sá,M.F.; Almeida,E.R.P.; Gander,E.S.; Rech,E.L.
Bean (Phaseolus vulgaris), an important component in the diet of people in developing countries, has low levels of the essential amino acid, methionine. We have attempted to correct this deficiency by introducing a transgene coding for a methionine-rich storage albumin from the Brazil nut via biolistic methods. The transgene's coding sequence was driven by a doubled 35S CaMV promoter and AMV enhancer sequences. The transgene was stable and correctly expressed in homozygous R2 to R5 seeds. In two of the five transgenic lines the methionine content was significantly increased (14 and 23%) over the values found in untransformed plants.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300026

Assessment of the genetic risks of a metallic alloy used in medical implants
Provedor de dados: Genetics and Molecular Biology
Autores: Gomes,Cristiano C.; Moreira,Leonardo M.; Santos,Vanessa J.S.V.; Ramos,Alfeu S.; Lyon,Juliana P.; Soares,Cristina P.; Santos,Fabio V.
The use of artificial implants provides a palliative or permanent solution for individuals who have lost some bodily function through disease, an accident or natural wear. This functional loss can be compensated for by the use of medical devices produced from special biomaterials. Titanium alloy (Ti-6Al-4V) is a well-established primary metallic biomaterial for orthopedic implants, but the toxicity of the chemical components of this alloy has become an issue of concern. In this work, we used the MTT assay and micronucleus assay to examine the cytotoxicity and genotoxicity, respectively, of an extract obtained from this alloy. The MTT assay indicated that the mitochondrial activity and cell viability of CHO-K1 cells were unaffected by exposure to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomaterial; Cytotoxicity; Micronucleus; Mutagenicity; Ti-6Al-4V.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100020

A preliminary survey of M. hyopneumoniae virulence factors based on comparative genomic analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Ferreira,Henrique Bunselmeyer; Castro,Luiza Amaral de.
Mycoplasma hyopneumoniae is the etiological agent of porcine enzootic pneumonia (PEP), a major problem for the pig industry. The mechanisms of M. hyopneumoniae pathogenicity allow to predict the existence of several classes of virulence factors, whose study has been essentially restricted to the characterization of adhesion-related and major antigenic proteins. The now available complete sequences of the genomes of two pathogenic and one non-pathogenic strain of M. hyopneumoniae allowed to use a comparative genomics approach to putatively identify virulence genes. In this preliminary survey, we were able to identify 118 CDSs encoding putative virulence factors, based on specific criteria ranging from predicted cell surface location or variation between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Porcine enzootic pneumoniae; Virulence; Host-pathogen interaction; Antigen; Adhesin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200012

Genetic parameters for five traits in Africanized honeybees using Bayesian inference
Provedor de dados: Genetics and Molecular Biology
Autores: Padilha,Alessandro Haiduck; Sattler,Aroni; Cobuci,Jaime Araújo; McManus,Concepta Margaret.
Heritability and genetic correlations for honey (HP) and propolis production (PP), hygienic behavior (HB), syrupcollection rate (SCR) and percentage of mites on adult bees (PMAB) of a population of Africanized honeybees were estimated. Data from 110 queen bees over three generations were evaluated. Single and multi-trait models were analyzed by Bayesian Inference using MTGSAM. The localization of the hive was significant for SCR and HB and highly significant for PP. Season-year was highly significant only for SCR. The number of frames with bees was significant for HP and PP, including SCR. The heritability estimates were 0.16 for HP, 0.23 for SCR, 0.52 for HB, 0.66 for PP, and 0.13 for PMAB. The genetic correlations were positive among productive traits...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heritability; Genetic correlations; Environmental effects; Apis mellifera.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200011

ERRATUM
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/report
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300028

Molecular characterization of  Chaetomium  species using URP-PCR
Provedor de dados: Genetics and Molecular Biology
Autores: Aggarwal,Rashmi; Sharma,Vandana; Kharbikar,Lalit L.; Renu,.
Chaetomium  spp. are common colonizers of soil and cellulose-containing substrates. Seventeen isolates of  Chaetomium  spp., which included 15 isolates of  C. globosum  and one each of  C. reflexum  and  C. perlucidum , were genetically characterized with universal rice primers (URP - primers derived from DNA repeat sequences in the rice genome) using polymerase chain reaction (URP-PCR). Out of the 12 URP's used in the study, nine primers were effective in producing polymorphic fingerprint patterns from DNA of  Chaetomium spp . Analysis of the entire fingerprint profile using the unweighted pair-group method with arithmetic averages (UPGMA) clearly differentiated  C. globosum  isolates from  C. perlucidum  and  C. reflexum . One of the primers, URP-2R,...
Palavras-chave: Chaetomium globosum; Chaetomium reflexum; Chaetomium perlucidum; Genetic variability; URP-PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500023

Expression, purification and DNA-binding activities of two putative ModE proteins of Herbaspirillum seropedicae (Burkholderiales, Oxalobacteraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,André L.F.; Chubatsu,Leda S.; Souza,Emanuel M.; Pedrosa,Fábio O.; Monteiro,Rose A.; Rego,Fabiane G.M.; Rigo,Liu U.
In prokaryotes molybdenum is taken up by a high-affinity ABC-type transporter system encoded by the modABC genes. The endophyte β-Proteobacterium Herbaspirillum seropedicae has two modABC gene clusters and two genes encoding putative Mo-dependent regulator proteins (ModE1 and ModE2). Analysis of the amino acid sequence of the ModE1 protein of H. seropedicae revealed the presence of an N-terminal domain containing a DNA-binding helix-turn-helix motif (HTH) and a C-terminal domain with a molybdate-binding motif. The second putative regulator protein, ModE2, contains only the helix-turn-helix motif, similar to that observed in some sequenced genomes. We cloned the modE1 (810 bp) and modE2 (372 bp) genes and expressed them in Escherichia coli as His-tagged...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbaspirillum seropedicae; ModE1 protein; ModE2 protein.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400022

Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae)
Provedor de dados: Genetics and Molecular Biology
Autores: Freitas,Patrícia Scotini; Andrade,Sérgio Faloni de; Maistro,Edson Luis.
The northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) is very rich in anthocyanins, natural pigments which have strong antioxidant properties and potential health benefits, resulting in the worldwide use the blueberry as a medicinal plant. We investigated the mutagenic potential of simple hydroalcoholic extracts of V. corymbosum acutely administrated by gavage to Swiss mice at doses of 1 g kg-1, 1.5 g kg-1 and 2 g kg-1. Peripheral blood cells were collected 4 h and 24 h post-gavage and assessed by the alkaline comet assay, with further blood samples being collected at 48 h and 72 h for assessment using the micronucleus (MN) assay. Our results show that the V. corymbosum extracts did not induce any statistically significant increase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Micronucleus; Mutagenicity; Vaccinium corymbosum.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300025

Conservation genetics of an endangered Catalonian cattle breed ("Alberes")
Provedor de dados: Genetics and Molecular Biology
Autores: Jordana,J.; Piedrafita,J.; Carre,X.; Martell,A.
We biochemically analyzed and characterized the genetic structure of a population in danger of extinction ,"Alberes", a local cattle breed of the Catalonian Pyrenees (Spain and France). Ninety-two individuals were analyzed for five polymorphic genetic loci (Hb, Alb, Tf, Gc and Ptf2). The animals were grouped according to coat color: Fagina Alberes variety (N = 39) and Black Alberes variety (N = 53). The genetic structures and relationships between these subpopulations and one "outgroup" breed ("Bruna dels Pirineus") were analyzed and compared by using F-statistics. We determined that inbreeding in the Alberes breed is not significant, since negative and nonsignificant FIT and FIS values were obtained. The average genetic differentiation between...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300017

Assessment of two medicinal plants, Psidium guajava L. and Achillea millefolium L., in in vitro and in vivo assays
Provedor de dados: Genetics and Molecular Biology
Autores: Teixeira,Rosangela de Oliveira; Camparoto,Marjori Leiva; Mantovani,Mário Sérgio; Vicentini,Veronica Elisa Pimenta.
The use of medicinal plants by the general population is an old and still widespread practice, which makes studies of their genotoxicity essential. Psidium guajava L. and Achillea millefolium L. are examples of plants commonly used in popular medicine. P. guajava L. is indicated for diarrhea and also as an antiseptic, while A. millefolium L. is indicated as an analgesic, antispasmodic, digestive, diuretic, antiseptic, astringent, emollient, wound healer and hemorrhoid medication. The aim of this study was to determine the effects of the infusions of these two plant species on chromosomes and the cell cycle. Leaves from the plants were used to prepare infusions, in the same manner as teas, but at two different concentrations. Allium cepa L. root-tip cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal herbs; Chromosome damage; Mutagenicity test.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400021

Molecular identification, phylogeny and geographic distribution of Brazilian mangrove oysters (Crassostrea)
Provedor de dados: Genetics and Molecular Biology
Autores: Melo,Aline Grasielle Costa de; Varela,Eduardo Sousa; Beasley,Colin Robert; Schneider,Horacio; Sampaio,Iracilda; Gaffney,Patrick Michael; Reece,Kimberly S.; Tagliaro,Claudia Helena.
Oysters (Ostreidae) manifest a high degree of phenotypic plasticity, whereby morphology is of limited value for species identification and taxonomy. By using molecular data, the aim was to genetically characterize the species of Crassostrea occurring along the Brazilian coast, and phylogenetically relate these to other Crassostrea from different parts of the world. Sequencing of the partial cytochrome oxidase c subunit I gene (COI), revealed a total of three species of Crassostrea at 16 locations along the Brazilian coast. C. gasar was found from Curuçá (Pará state) to Santos (São Paulo state), and C. rhizophorae from Fortim (Ceará state) to Florianópolis (Santa Catarina state), although small individuals of the latter species were also found at Ajuruteua...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic identification; Oysters; Biogeography; COI gene; Ostreidae.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300030

Allozyme variation and sociogenetic structure of Polistes satan Bequaert 1940 colonies (Hymenoptera, Vespidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Gaspar,Jane Cristina Wehren; López-Uribe,Margarita María; Del Lama,Marco Antônio.
We collected 40 colonies of the wasp Polistes satan in November 1993 (Population A = 23 colonies) and October 1995 (Population B = 17 colonies) from the town of Delfinopólis in the Brazilian state of Minas Gerais. Pupae from these nests were used to assess allozyme variation and genetic relatedness among nestmates. Of the 24 gene loci sampled six showed allozyme polymorphism (Est-2, Pgm-1, 6Pgd, Hk-1, Idh and Pep-A) and all loci except Idh were at Hardy-Weinberg equilibrium. Populations A and B showed distinct intra-loci heterozygosities, but similar average heterozygosity over all loci. Significant inter-population differentiation (Fst = 0.051; p &lt; 0.01) was detected, probably due to genetic drift driven by bottlenecking that occurred due to the first...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allozymes; Gene variation; Polistes satan; Relatedness; Wasps.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300028

AFLP marker linked to water-stress-tolerant bulks in barley (Hordeum vulgare L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Altinkut,A.; Kazan,K.; Gozukirmizi,N.
The amplified fragment length polymorphism (AFLP) assay is an efficient method for the identification of molecular markers, useful in the improvement of numerous crop species. Bulked Segregant Analysis (BSA) was used to identify AFLP markers associated with water-stress tolerance in barley, as this would permit rapid selection of water-stress tolerant genotypes in breeding programs. AFLP markers linked to water-stress tolerance was identified in two DNA pools (tolerant and sensitive), which were established using selected F2 individuals resulting from a cross between water-stress-tolerant and sensitive barley parental genotypes, based on their paraquat (PQ) tolerance, leaf size, and relative water content (RWC). All these three traits were previously shown...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bulked segregant analysis; Drought; Hordeum vulgare; AFLP; Paraquat.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100013

Integrated analysis of the critical region 5p15.3–p15.2 associated with cri-du-chat syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Corrêa,Thiago; Feltes,Bruno César; Riegel,Mariluce.
Abstract Cri-du-chat syndrome (CdCs) is one of the most common contiguous gene syndromes, with an incidence of 1:15,000 to 1:50,000 live births. To better understand the etiology of CdCs at the molecular level, we investigated theprotein–protein interaction (PPI) network within the critical chromosomal region 5p15.3–p15.2 associated with CdCs using systemsbiology. Data were extracted from cytogenomic findings from patients with CdCs. Based on clinical findings, molecular characterization of chromosomal rearrangements, and systems biology data, we explored possible genotype–phenotype correlations involving biological processes connected with CdCs candidate genes. We identified biological processes involving genes previously found to be associated with CdCs,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cri-du-Chat Syndrome; 5p– cytogenomics; Integrative Analysis; PPI; Systems biology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200186

Genetic variability in Amerindian populations of Northern Argentina
Provedor de dados: Genetics and Molecular Biology
Autores: Dejean,Cristina B.; Crouau-Roy,Brigitte; Goicoechea,Alicia S.; Avena,Sergio A.; Carnese,Francisco R.
The allelic variability of four dinucleotide microsatellites located in the HLA region (MOGc, D6S265, MIB, and TNFa) was analyzed in 67 individuals representing three Amerindian populations of the Argentine Gran Chaco: Toba, Wichi and Chorote. Genomic DNA was prepared from peripheral blood and DNA was extracted using the standard phenol-chloroform procedure. Alleles were identified by PCR, using an end-labelled reverse oligonucleotide primer (fluorescent 6 - Fam labeling). Despite the low number of samples studied, a high level of gene diversity was observed in each population and for each locus. Moreover, the mean number of alleles was 7.7, 5.3, 10.0, and 7.0 at loci MOGc, D6S265, MIB and TNFa, respectively. Differentiation tests between pairs of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aboriginal Argentinians; Microsatellites; Intra- and inter-populational variation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400004

Restriction-modification systems in Mycoplasma spp
Provedor de dados: Genetics and Molecular Biology
Autores: Brocchi,Marcelo; Vasconcelos,Ana Tereza Ribeiro de; Zaha,Arnaldo.
Restriction and Modification (R-M) systems are present in all Mycoplasma species sequenced so far. The presence of these genes poses barriers to gene transfer and could protect the cell against phage infections. The number and types of R-M genes between different Mycoplasma species are variable, which is characteristic of a polymorphism. The majority of the CDSs code for Type III R-M systems and particularly for methyltransferase enzymes, which suggests that functions other than the protection against the invasion of heterologous DNA may exist. A possible function of these enzymes could be the protection against the invasion of other but similar R-M systems. In Mycoplasma hyopneumoniae strain J, three of the putative methyltransferase genes were clustered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Restriction-modification systems; Mycoplasma spp; Genomes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200011

Tandem repeat distribution of gene transcripts in three plant families
Provedor de dados: Genetics and Molecular Biology
Autores: Maia,Luciano Carlos da; Souza,Velci Queiróz de; Kopp,Mauricio Marini; Carvalho,Fernando Irajá Félix de; Oliveira,Antonio Costa de.
Tandem repeats (microsatellites or SSRs) are molecular markers with great potential for plant genetic studies. Modern strategies include the transfer of these markers among widely studied and orphan species. In silico analyses allow for studying distribution patterns of microsatellites and predicting which motifs would be more amenable to interspecies transfer. Transcribed sequences (Unigene) from ten species of three plant families were surveyed for the occurrence of micro and minisatellites. Transcripts from different species displayed different rates of tandem repeat occurrence, ranging from 1.47% to 11.28%. Both similar and different patterns were found within and among plant families. The results also indicate a lack of association between genome size...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; EST; Comparative genomics; Molecular markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400025

Antonio Carlos Garros Stort (1939-2005)
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Lionel Segui.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300001

Evidence of high inbreeding in a population of the endangered giant anteater, Myrmecophaga tridactyla (Myrmecophagidae), from Emas National Park, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Collevatti,Rosane G.; Leite,Kelly C.E.; Miranda,Guilherme H.B. de; Rodrigues,Flavio H.G.
We report the genetic structure, relatedness and mating structure of a population of the endangered giant anteater Myrmecophaga tridactyla Linnaeus, 1758 in the Emas National Park, Brazil, based on variability at five microsatellite loci. Additionally, we addressed the hypothesis that the M. tridactyla population studied has low levels of polymorphism and high levels of inbreeding and relatedness and that animals with overlapping home range are highly related. All five microsatellite loci displayed low levels of polymorphism and of expected and observed heterozygosity. The low level of polymorphism and high inbreeding showed by the population studied may be the outcome of high mortality and reduction in population size due to recurrent fire events in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado; Conservation; Microsatellites; Phylopatry; Relatedness.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100020

Association of homocysteine and methylene tetrahydrofolate reductase (MTHFR C677T) gene polymorphism with coronary artery disease (CAD) in the population of North India
Provedor de dados: Genetics and Molecular Biology
Autores: Tripathi,Rajneesh; Tewari,Satyendra; Singh,Prabhat Kumar; Agarwal,Sarita.
The implications of the methylene tetrahydrofolate reductase (MTHFR) gene and the level of homocysteine in the pathogenesis of coronary artery disease (CAD) have been extensively studied in various ethnic groups. Our aim was to discover the association of MTHFR (C677T) polymorphism and homocysteine level with CAD in north Indian subjects. The study group consisted of 329 angiographically proven CAD patients, and 331 age and sex matched healthy individuals as controls. MTHFR (C677T) gene polymorphism was detected based on the polymerase chain reaction and restriction digestion with HinfI. Total homocysteine plasma concentration was measured using immunoassay. T allele frequency was found to be significantly higher in patients than in the control group. We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiography; CAD; Homocysteine; MTHFR polymorphism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200004

Analysis of the interaction between human kidney anion exchanger 1 and kanadaptin using yeast two-hybrid systems
Provedor de dados: Genetics and Molecular Biology
Autores: Wongthida,Phonphimon; Akkarapatumwong,Varaporn; Limjindaporn,Thawornchai; Kittanakom,Saranya; Keskanokwong,Thitima; Eurwilaichitr,Lily; Yenchitsomanus,Pa-thai.
Kidney anion exchanger adaptor protein (Kanadaptin) is a protein which interacts with the cytoplasmic N-terminal domain of kidney anion exchanger 1 (kAE1) and was first detected in mice using the yeast two-hybrid system and was also found to co-localize with kAE1 in rabbit a-intercalated cells. Impaired trafficking of human kAE1 can result in the kidney disease-distal renal tubular acidosis (dRTA), and defective interaction between human kAE1 and kanadaptin may cause this trafficking impairment and be the basis for dRTA pathogenesis. However, it is unknown whether kAE1 can really interact with kanadaptin in humans. We have thus investigated the interaction between human kAE1 and human kanadaptin by using both Gal4 and LexA yeast two-hybrid systems. It was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney anion exchanger 1; Kanadaptin; Yeast two-hybrid system.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100003

Survey of transposable elements in sugarcane expressed sequence tags (ESTs)
Provedor de dados: Genetics and Molecular Biology
Autores: Rossi,Magdalena; Araujo,Paula Gonçalves; Van Sluys,Marie-Anne.
The sugarcane expressed sequence tag (SUCEST) project has produced a large number of cDNA sequences from several plant tissues submitted or not to different conditions of stress. In this paper we report the result of a search for transposable elements (TEs) revealing a surprising amount of expressed TEs homologues. Of the 260,781 sequences grouped in 81,223 fragment assembly program (Phrap) clusters, a total of 276 clones showed homology to previously reported TEs using a stringent cut-off value of e-50 or better. Homologous clones to Copia/Ty1 and Gypsy/Ty3 groups of long terminal repeat (LTR) retrotransposons were found but no non-LTR retroelements were identified. All major transposon families were represented in sugarcane including Activator (Ac),...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100020

The use of PCR-RFLP as an identification tool for three closely related species of rodents of the genus Akodon (Sigmodontinae, Akodontini)
Provedor de dados: Genetics and Molecular Biology
Autores: Fagundes,Valéria; Nogueira,Cristina Dornelas de Andrade.
Three cryptic species of the rodent Akodon (A. cursor, A. montensis and Akodon sp.) were analyzed. The two former species are sympatric in the Brazilian states of São Paulo, Rio de Janeiro and Minas Gerais, where hybrids have already been found. The third species, Akodon sp., occurs in an isolated area in Central Brazil. The identification of these species is difficult by the need of living animals. At present, karyotyping is the only method used in the identification of specimens. We used PCR-RFLP of the mitochondrial cytochrome gene to test the distinctiveness of the three species, which was confirmed by the absence of shared species-specific haplotypes. We also detected a geographical pattern of haplotypes distribution with highly polymorphic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Akodon cursor; Akodon montensis; PCR-RFLP; Sigmodontinae.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400031

Effects of Pterocaulon polystachyum DC. (Asteraceae) on onion (Allium cepa) root-tip cells
Provedor de dados: Genetics and Molecular Biology
Autores: Knoll,Michelle Frainer; Silva,Antonio Carlos Ferreira da; Canto-Dorow,Thais Scotti do; Tedesco,Solange Bosio.
In Brazil leaf infusions of the plant Pterocaulon polystachyum DC (Asteraceae) are used to treat digestive problems. We used plants from six P. polystachyum populations to prepare fresh aqueous leaf infusions at the concentration normally used in Brazil (2.5 g L-1) and at twice (5 g L-1) and four times (10 g L-1) this concentration. We rooted onion bulbs in a water control or one of the infusions for 24 h and used the in vivo onion (Allium cepa) root-tip cell test to investigate the potential mutagenicity of the infusions by calculating the mitotic index for the control and the experimental treatments and comparing them using the Chi-squared test (chi2, p = 0.05). There was a highly significant decrease in the mitotic index of root-tip cells treated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Mutagenicity; Onion (Allium cepa) test; Pterocaulon polystachyum.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300024

Differential gene expression profiles in peripheral blood in Northeast Chinese Han people with acute myocardial infarction
Provedor de dados: Genetics and Molecular Biology
Autores: Fan,Lin; Meng,Heyu; Guo,Xudong; Li,Xiangdong; Meng,Fanbo.
Abstract This study aimed to use gene chips to investigate differential gene expression profiles in the occurrence and development of acute myocardial infarction (AMI). The study included 12 AMI patients and 12 healthy individuals. Total mRNA of peripheral bloodwas extracted and reversed-transcribed to cDNA for microarray analysis. After establishing two pools with three subjects each (3 AMI patients and 3 healthy individuals), the remaining samples were used for RT-qPCR to confirm the microarray data. From the microarray results, seven genes were randomly selected for RT-qPCR. RT-qPCR results were analyzed by the 2-ΔΔCt method. Microarray analysis showed that 228 genes were up- regulated and 271 were down-regulated (p ≤ 0.05, |logFC| &gt; 1). Gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myocardial infarction; RNA; Differential expression.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100059

Genetic structure and conservation of Mountain Lions in the South-Brazilian Atlantic Rain Forest
Provedor de dados: Genetics and Molecular Biology
Autores: Castilho,Camila S.; Marins-Sá,Luiz G.; Benedet,Rodrigo C.; Freitas,Thales R.O.
The Brazilian Atlantic Rain Forest, one of the most endangered ecosystems worldwide, is also among the most important hotspots as regards biodiversity. Through intensive logging, the initial area has been reduced to around 12% of its original size. In this study we investigated the genetic variability and structure of the mountain lion, Puma concolor. Using 18 microsatellite loci we analyzed evidence of allele dropout, null alleles and stuttering, calculated the number of allele/locus, PIC, observed and expected heterozygosity, linkage disequilibrium, Hardy-Weinberg equilibrium, F IS, effective population size and genetic structure (MICROCHECKER, CERVUS, GENEPOP, FSTAT, ARLEQUIN, ONESAMP, LDNe, PCAGEN, GENECLASS software),we also determine whether there...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucaria Forest; Atlantic Rain Forest; Conservation; Genetic diversity; Microsatellite.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100009

Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products
Provedor de dados: Genetics and Molecular Biology
Autores: Schneider,Marielle Cristina; Carraro,Bianca Pierina; Cella,Doralice Maria; Matiello,Rodrigo Rodrigues; Artoni,Roberto Ferreira; Almeida,Mara Cristina.
In this work we describe the cytogenetic analyses performed in specimens of Astylus variegatus (Germar, 1824) collected in two localities: one area of natural vegetation and one of agricultural crops, where agrochemical products were used. Astylus variegatus had karyotypes with 2n(male) = 16+Xy p and 2n (female) = 16+XXp, with exclusively metacentric chromosomes. Pachytene spermatocytes showed synapsed autosomal bivalents and non-associated sex chromosomes. In diplotene, the autosomal bivalents exhibited one or two terminal chiasmata and the Xy p had a typical parachute configuration. In meiotic cells of some specimens, an extra chromosome, interpreted as a B chromosome, was observed. C-banding showed constitutive heterochromatin in the pericentromeric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B chromosome; Constitutive heterochromatin; Karyotype; Meiosis; Ag-NORs.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400023

The chitinase C gene PsChiC from Pseudomonas sp. and its synergistic effects on larvicidal activity
Provedor de dados: Genetics and Molecular Biology
Autores: Zhong,Wanfang; Ding,Shaojun; Guo,Huifang.
Pseudomonas sp. strain TXG6-1, a chitinolytic gram-negative bacterium, was isolated from a vegetable field in Taixing city, Jiangsu Province, China. In this study, a Pseudomonas chitinase C gene (PsChiC) was isolated from the chromosomal DNA of this bacterium using a pair of specific primers. The PsChiC gene consisted of an open reading frame of 1443 nucleotides and encoded 480 amino acid residues with a calculated molecular mass of 51.66 kDa. The deduced PsChiC amino acid sequence lacked a signal sequence and consisted of a glycoside hydrolase family 18 catalytic domain responsible for chitinase activity, a fibronectin type III-like domain (FLD) and a C-terminal chitin-binding domain (ChBD). The amino acid sequence of PsChiCshowed high sequence homology...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalytic domain; Chitin-binding domain; Fibronectin type-like domain; Gene cloning; Insecticidal activity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300366

Mechanisms involved in the induction of aneuploidy: the significance of chromosome loss
Provedor de dados: Genetics and Molecular Biology
Autores: Seoane,A.I.; Güerci,A.M.; Dulout,F.N.
The induction of aneuploidy by physical and chemical agents using different test systems was evaluated. The effect of X-rays, caffeine, acetaldehyde, ethanol, diethylstilbestrol, propionaldehyde, and chloral hydrate was studied by chromosome counting in Chinese hamster embryonic diploid cells. Aneugenic ability of cadmium chloride, cadmium sulfate, potassium dichromate, chromium chloride, nickel chloride, and nickel sulfate was assessed by means of anaphase-telophase analysis in Chinese hamster ovary cells. Chromosome counting in human fibroblasts (MRC-5 cell line) was employed to evaluate the effect of cacodilic acid, cadmium chloride, cadmium sulfate, and potassium dichromate. Finally, the induction of kinetochore-positive and kinetochore negative...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400054

Mapping of quantitative trait loci and confirmation of the FAT1 region on chromosome 4 in an F2 population of pigs
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Kleibe de Moraes; Paixão,Débora Martins; Silva,Priscila Vendramini; Solero,Bruna Pena; Pereira,Mario Sérgio; Lopes,Paulo Sávio; Guimarães,Simone Eliza Facioni.
The objective was to map QTL on porcine chromosome 4 and to associate them with carcass and internal organ traits in an F2 population. The F1 population was produced by outbreed crossing, using two native Brazilian breed Piau boars and 18 commercial sows. A total of 617 F2 animals issued from 11 F1 boars and 54 F1 sows were typed for a total of five microsatellite markers. The data were analyzed by multiple regressions developed for the analysis of crosses between outbred lines, using the QTL Express software. Significant evidence for QTL was found for pig chromosome 4 regarding carcass and internal organ traits. All QTL were detected in the same region of the chromosome, designated FAT1.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatness; Pig production; Divergent crosses; Microsatellite markers; QTL mapping.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300013

Editorial - Special section on Bioinformatics
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Luciano da Fontoura; Cesar Jr,Roberto Marcondes; Barrera,Junior.
Tipo: Info:eu-repo/semantics/other
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400001

Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Bombonato,Juliana Rodrigues; Bonatelli,Isabel Aparecida Silva; Silva,Gislaine Angélica Rodrigues; Moraes,Evandro Marsola; Zappi,Daniela Cristina; Taylor,Nigel P.; Franco,Fernando Faria.
Abstract The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cactaceae; Cereus; Cross-genera; SSR markers; Transferability.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100087

The complex translocation (9;14;14) involving IGH and CEBPE genes suggests a new subgroup in B-lineage acute lymphoblastic leukemia
Provedor de dados: Genetics and Molecular Biology
Autores: Zerrouki,Rachid; Benhassine,Traki; Bensaada,Mustapha; Lauzon,Patricia; Trabzi,Anissa.
Abstract Many subtypes of acute lymphoblastic leukemia (ALL) are associated with specific chromosomal rearrangements. The complex translocation t(9;14;14), a variant of the translocation (14;14)(q11;q32), is a rare but recurrent chromosomal abnormality involving the immunoglobulin heavy-chain (IGH) and CCAAT enhancer-binding protein (CEBPE) genes in B-lineage ALL (B-ALL) and may represent a new B-ALL subgroup. We report here the case of a 5-year-old girl with B-ALL, positive for CD19, CD38 and HLA-DR. A direct technique and G-banding were used for chromosomal analysis and fluorescentin situ hybridization (FISH) with BAC probes was used to investigate a possible rearrangement of the IGH andCEBPE genes. The karyotype exhibit the chromosomal aberration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute lymphoblastic leukemia; CEBPE; FISH; IGH; Translocation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100007

A homolog of the RPS2 disease resistance gene is constitutively expressed in Brassica oleracea
Provedor de dados: Genetics and Molecular Biology
Autores: Malvas,Celia C.; Melotto,Maeli; Truffi,Daniela; Camargo,Luis E.A.
In this study, we identified disease resistance gene homologs in Brassica oleracea and assessed their expression in lines resistant and susceptible to Xanthomonas campestris pv. campestris (Xcc). Two DNA fragments of approximately 2.5 kb (BI-16/RPS2 and Lc201/RPS2) were amplified by PCR from two Brassica lines using primers based on an RPS2 homologous sequence previously described in the Brassica oleracea ecotype B117. The sequences of these fragments shared high similarity (95-98%) with RPS2 homologs from various Brassica species. The digestion of these fragments with restriction enzymes revealed polymorphisms at the Xba I restriction sites. The length polymorphisms were used as a co-dominant marker in an F2 population developed to segregate for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xanthomonas campestris pv. campestris; Disease resistance gene homolog; Black rot.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400015

Discrimination of Shark species by simple PCR of 5S rDNA repeats
Provedor de dados: Genetics and Molecular Biology
Autores: Pinhal,Danillo; Gadig,Otto BF; Wasko,Adriane P; Oliveira,Claudio; Ron,Ernesto; Foresti,Fausto; Martins,Cesar.
Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chondrichthyes; PCR; Species identification; 5S rDNA; Sharks.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200033

Profile of micronucleus frequencies and DNA damage in different species   of fish in a eutrophic tropical lake
Provedor de dados: Genetics and Molecular Biology
Autores: Grisolia,Cesar K.; Rivero,Carla L. G.; Starling,Fernando L. R. M.; Silva,Izabel C. R. da; Barbosa,Antonio C.; Dorea,Jose G.
Lake Paranoá is a tropical reservoir for the City of Brasilia, which became eutrophic due to inadequate sewage treatment associated with intensive population growth. At present, two wastewater treatment plants are capable of processing up to 95% of the domestic sewage, thereby successfully reducing eutrophization. We evaluated both genotoxic and cytotoxic parameters in several fish species ( Geophagus brasiliensis ,  Cichla temensis ,  Hoplias malabaricus ,  Astyanax bimaculatus lacustres ,  Oreochromis niloticus ,  Cyprinus carpio  and  Steindachnerina insculpita ) by using the micronucleus (MN) test, the comet assay and nuclear abnormality assessment in peripheral erythrocytes. The highest frequencies of MN were found in  Cichla temensis  and  Hoplias...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Fish biomonitoring; Eutrophic lake; Micronucleus; Comet assay; Cytotoxicity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100021

Genetic parameters and selection strategies for soybean genotypes resistant to the stink bug-complex
Provedor de dados: Genetics and Molecular Biology
Autores: Godoi,Cláudio Roberto Cardoso de; Pinheiro,José Baldin.
Soybean genotypes resistant to stink bugs are derived from complex breeding processes obtained through indirect selection. The aim of the present work was to estimate genetic parameters for guiding selection strategies towards resistant genotypes, based on those traits associated with responses to pod-attacking stink bugs, such as the grain filling period (GFP), leaf retention (LR), percentage index of pod damage (PIPD) and percentage of spotted seeds (PSS). We assessed the parental lines IAC-100 (resistant) and FT-Estrela (susceptible), the progenies F2 and F4, 30 progenies F2:3, 30 progenies BC1F2:3 and 30 progenies BC2F2:3, besides the cultivars BRS Celeste and MGBR-46 (Conquista). Three field experiments, using randomized complete block design with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Insect resistance; Stink bugs; Heritability.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200020

Astyanax hastatus Myers, 1928 (Teleostei, Characidae): a new species complex within the genus Astyanax?
Provedor de dados: Genetics and Molecular Biology
Autores: Kavalco,Karine Frehner; Brandão,Karina de Oliveira; Pazza,Rubens; Almeida-Toledo,Lurdes Foresti de.
Four populations of Astyanax hastatus Myers 1928 from the Guapimirim River basin (Rio de Janeiro State) were analyzed and three distinct cytotypes identified. These cytotypes presented 2n = 50 chromosomes, with 4M+8SM+10ST+28A (Cytotype A), 8M+10SM+14ST+18A (Cytotype B), 6M+8SM+4ST+32A (Cytotype C) and scanty heterochromatin, mainly located throughout pericentromeric regions of several chromosomal pairs. No homologies with the As-51 satellite DNA were observed in the three cytotypes, although all of them presented multiple 18S rDNA sites, as detected by both silver nitrate staining and FISH (fluorescent in situ hybridization). The application of the term "species complex" in Astyanax is discussed from a cytotaxonomic viewpoint.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astyanax hastatus; Molecular cytogenetics; Karyotypic evolution; Species complex.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300009

Assessment of genetic diversity in Brazilian barley using SSR markers
Provedor de dados: Genetics and Molecular Biology
Autores: Ferreira,Jéssica Rosset; Pereira,Jorge Fernando; Turchetto,Caroline; Minella,Euclydes; Consoli,Luciano; Delatorre,Carla Andréa.
Abstract Barley is a major cereal grown widely and used in several food products, beverage production and animal fodder. Genetic diversity is a key component in breeding programs. We have analyzed the genetic diversity of barley accessions using microsatellite markers. The accessions were composed of wild and domesticated barley representing genotypes from six countries and three breeding programs in Brazil. A total of 280 alleles were detected, 36 unique to Brazilian barley. The marker Bmag120 showed the greatest polymorphism information content (PIC), with the highest mean value found on chromosome three, and the lowest on chromosomes four and six. The wild accessions presented the highest diversity followed by the foreign genotypes. Genetic analysis was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Domesticated barley; Genetic diversity; Hordeum vulgare ssp. vulgare; Hordeum vulgare ssp. spontaneum; Microsatellite markers.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100086

Effects of Maytenus ilicifolia Mart. and Bauhinia candicans Benth infusions on onion root-tip and rat bone-marrow cells
Provedor de dados: Genetics and Molecular Biology
Autores: Camparoto,Marjori Leiva; Teixeira,Rosangela de Oliveira; Mantovani,Mário Sérgio; Vicentini,Veronica Elisa Pimenta.
Medicinal plants are widely used to treat various diseases, and in Brazil the plants Maytenus ilicifolia Mart. and Bauhinia candicans Benth are commonly used in popular medicine. However, there are a large number of compounds in plants which can produce alterations in genetic material, and this study was conducted to investigate any possible mutagenic and cytotoxic effects that M. ilicifolia and B. candicans infusions may have on the cell cycle and chromosomes. Infusions were prepared with in natura leaves to give two concentrations of infusions, one at the concentration normally used by the population in general and the other at 10 times this value (i.e. 3.5 and 35 mg/mL for M. ilicifolia and 0.465 and 4.65 mg/mL for B. candicans). Onion (Allium cepa L.)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa test; Bauhinia candicans; Chromosome damage; Maytenus ilicifolia; Medicinal herbs; Mutagenicity test; Wistar rat bone marrow cells.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100016

Oxidative stress assessment by glutathione peroxidase activity and glutathione levels in response to selenium supplementation in patients with Mucopolysaccharidosis I, II and VI
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira-Silva,José Araújo de; Yamamoto,Joyce Umbelino Pinto; Oliveira,Renata Bernardes de; Monteiro,Vaneisse Cristina Lima; Frangipani,Beatriz Jurkiewcz; Kyosen,Sandra Obikawa; Martins,Ana Maria; D’Almeida,Vânia.
Abstract We assessed levels of plasma selenium (Se), selenoproteins and their change after Se supplementation in patients with mucopolysaccharidosis (MPS) types I, II and VI. This was done in a retrospective study of the medical records of 30 patients with MPS I (n=13), MPS II (n=9) and MPS VI (n=8) who were being treated with enzyme replacement therapy. As part of routine nutritional monitoring, Se levels were measured, revealing that 28 patients (93.3%) had values below the normal range. Therefore, they received supplementation for 12 months, and Se was measured after 6 and 12 months. Glutathione peroxidase (GPx) activity, total glutathione (GSHt), oxidized glutathione (GSSG) and reduced glutathione (GSH) were measured at baseline and 6 months after Se...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucopolysaccharidosis; Oxidative stress; Selenium; Glutathione peroxidase.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100001

A new member of the chalcone synthase (CHS) family in sugarcane
Provedor de dados: Genetics and Molecular Biology
Autores: Contessotto,Miriam G.G.; Monteiro-Vitorello,Claudia B.; Mariani,Pilar D.S.C.; Coutinho,Luiz L.
Sequences from the sugarcane expressed sequence tag (SUCEST) database were analyzed based on their identities to genes encoding chalcone-synthase-like enzymes. The sorghum (Sorghum bicolor) chalcone-synthase (CHS, EC 2.3.1.74) protein sequence (gi|12229613) was used to search the SUCEST database for clusters of sequencing reads that were most similar to chalcone synthase. We found 121 reads with homology to sorghum chalcone synthase, which we were then able to sort into 14 clusters which themselves were divided into two groups (group 1 and group 2) based on the similarity of their deduced amino acid sequences. Clusters in group 1 were more similar to the sorghum enzyme than those in group 2, having the consensus sequence of the active site of chalcone and...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100034

Chromosome number in Brazilian germplasm accessions of Paspalum hydrophilum, P. modestum and P. palustre (Gramineae; Paniceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Pozzobon,Marisa T.; Valls,José F.M.
This paper compiles results of chromosome counts of Paspalum hydrophilum, P. modestum and P. palustre. Four Brazilian accessions of P. modestum have shown 2n = 2x = 20 chromosomes, a number already found in one accession from Argentina and in two from Brazil. Three other Brazilian accessions showed tetraploid level (2n = 4x = 40), which was previously unknown in this species. In P. hydrophilum, only one of the accessions analyzed presented tetraploid level, initially established for the species from plants collected in Argentina. Five additional accessions from Brazil showed the diploid number, previously detected in a single Brazilian population. A tetraploid cytotype was found in P. palustre, previously known as a diploid species. In addition to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grass; Ploidy level; Paspalum hydrophilum; P. modestum; P. palustre.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300023

Multiple introductions and gene flow in subtropical South American populations of the fireweed, Senecio madagascariensis(Asteraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Mäder,Geraldo; Castro,Luana; Bonatto,Sandro Luis; Freitas,Loreta Brandão de.
Abstract Non-indigenous plants exhibit different attributes that make them aggressive competitors with indigenous plants and serious threats to biodiversity.Senecio madagascariensis (fireweed, Asteraceae), a native from southern Africa, is a strong competitor in agricultural activities and has toxic alkaloids that may result in high cattle mortality. In Brazil, this weed was collected for the first time in 1995 and has since spread quickly throughout the Pampas region. To better understand the invasion of the fireweed in South America, we used a genetic characterization with internal transcribed spacer (ITS) and microsatellite markers. Based on the ITS data, the southern Brazil populations of S. madagascariensis shared genetic homology with samples taken...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; ITS; Pampas; Weed; Spread.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100135

Plant senescence and proteolysis: two processes with one destiny
Provedor de dados: Genetics and Molecular Biology
Autores: Diaz-Mendoza,Mercedes; Velasco-Arroyo,Blanca; Santamaria,M. Estrella; González-Melendi,Pablo; Martinez,Manuel; Diaz,Isabel.
Abstract Senescence-associated proteolysis in plants is a complex and controlled process, essential for mobilization of nutrients from old or stressed tissues, mainly leaves, to growing or sink organs. Protein breakdown in senescing leaves involves many plastidial and nuclear proteases, regulators, different subcellular locations and dynamic protein traffic to ensure the complete transformation of proteins of high molecular weight into transportable and useful hydrolysed products. Protease activities are strictly regulated by specific inhibitors and through the activation of zymogens to develop their proteolytic activity at the right place and at the proper time. All these events associated with senescence have deep effects on the relocation of nutrients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Barley; Cysteine-proteases; Leaf senescence; Protein traffic; Protein recycling; Proteolysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300329

Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,A.S.G.; Guidelli,A.M.; Abreu,I.L.; Lemos,M.V.F.
PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: REP; ERIC; Crystal protein; Parasporal bodies.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200017

Dyslexia risk variant rs600753 is linked with dyslexia-specific differential allelic expression of DYX1C1
Provedor de dados: Genetics and Molecular Biology
Autores: Müller,Bent; Boltze,Johannes; Czepezauer,Ivonne; Hesse,Volker; Wilcke,Arndt; Kirsten,Holger.
Abstract An increasing number of genetic variants involved in dyslexia development were discovered during the last years, yet little is known about the molecular functional mechanisms of these SNPs. In this study we investigated whether dyslexia candidate SNPs have a direct, disease-specific effect on local expression levels of the assumed target gene by using a differential allelic expression assay. In total, 12 SNPs previously associated with dyslexia and related phenotypes were suitable for analysis. Transcripts corresponding to four SNPs were sufficiently expressed in 28 cell lines originating from controls and a family affected by dyslexia. We observed a significant effect of rs600753 on expression levels of DYX1C1 in forward and reverse sequencing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dyslexia; SNP; EQTL; Differential allelic expression.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100041

Prediction of hybrid means from a partial circulant diallel table using the ordinary least square and the mixed model methods
Provedor de dados: Genetics and Molecular Biology
Autores: Reis,Américo José dos Santos; Chaves,Lázaro José; Duarte,João Batista; Brasil,Edward Madureira.
By definition, the genetic effects obtained from a circulant diallel table are random. However, because of the methods of analysis, those effects have been considered as fixed. Two different statistical approaches were applied. One assumed the model to be fixed and obtained solutions through the ordinary least square (OLS) method. The other assumed a mixed model and estimated the fixed effects (BLUE) by generalized least squares (GLS) and the best linear unbiased predictor (BLUP) of the random effects. The goal of this study was to evaluate the consequences when considering these effects as fixed or random, using the coefficient of correlation between the responses of observed and non-observed hybrids. Crossings were made between S1 inbred lines from two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diallel analysis; BLUP; Prediction; Cross-validation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200023

Relationships between chromatin remodeling and DNA damage repair induced by 8-methoxypsoralen and UVA in yeast Saccharomyces cerevisiae
Provedor de dados: Genetics and Molecular Biology
Autores: Cruz,Lavínia Almeida; Guecheva,Temenouga Nikolova; Bonato,Diego; Henriques,João Antônio Pêgas.
Eukaryotic cells have developed mechanisms to prevent genomic instability, such as DNA damage detection and repair, control of cell cycle progression and cell death induction. The bifunctional compound furocumarin 8-methoxy-psoralen (8-MOP) is widely used in the treatment of various inflammatory skin diseases. In this review, we summarize recent data about the role of chromatin remodeling in the repair of DNA damage induced by treatment with 8-methoxypsoralen plus UVA (8-MOP+UVA), focusing on repair proteins in budding yeast Saccharomyces cerevisiae, an established model system for studying DNA repair pathways. The interstrand crosslinks (ICL) formed by the 8-MOP+UVA treatment are detrimental lesions that can block transcription and replication, leading to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA repair; Psoralen; Chromatin remodeling; Histones; DNA interstrand cross-links.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600021

The FMR1 premutation as a cause of premature ovarian failure in Brazilian women
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Silvia S.; Fonseca,Angela M. da; Bagnoli,Vicente R.; Vianna-Morgante,Angela M.
The loss-of-function mutation of the FMR1 gene due to expansion of the 5' UTR CGG repeat causes the fragile X syndrome, the most frequent form of inherited mental retardation. On the other hand, the FMR1 premutation, which is transcriptionally active and produces the protein, confers an increased risk for premature ovarian failure (POF) to carrier females. Among 41 unrelated Brazilian women with idiopathic POF, we found three carriers of premutations (CGG expansionse &gt; 59 repeats) and two carriers of high-intermediate alleles (50-55 repeats). Two premutations and two intermediate alleles were detected among the 16 familial POF cases, and one premutated woman, among the 25 sporadic cases. The premutation frequency among the familial cases (12.5%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Premature ovarian failure; Menopause; FMR1 premutation; Fragile X syndrome.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300002

Molecular marker heterozygosities and genetic distances as correlates of production traits in F1 bovine crosses
Provedor de dados: Genetics and Molecular Biology
Autores: Tambasco-Talhari,Daniella; Alencar,Maurício Mello de; Paz,Cláudia Cristina Paro de; Cruz,Geraldo Maria da; Rodrigues,Armando de Andrade; Packer,Irineu Umberto; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida.
Several studies have investigated the relationship between heterozygosity, genetic distance and production traits. The objective of the present study was to evaluate the influence of the degree of heterozygosity and genetic distance on growth, carcass and reproductive related features in F1 bovine crosses. We tested 10 polymorphic markers in 330 purebred cattle (Nelore, Canchim, Aberdeen Angus and Simental) and 256 crossbred cattle belonging to four crossbred groups. Individual heterozygosities (Hi) and multilocus genetic similarity (Dm) were estimated and used in correlation analysis against individual phenotypic measurements. Significant (p &lt; 0.05) Hi effects occurred for birth weight, 15 to 18 month weight, hot carcass weight and longissimus rib eye...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine crosses; Molecular markers; Heterozygosity; Genetic distance; Production traits.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200007

Costs of genetic testing: Supporting Brazilian Public Policies for the incorporating of molecular diagnostic technologies
Provedor de dados: Genetics and Molecular Biology
Autores: Schlatter,Rosane Paixão; Matte,Ursula; Polanczyk,Carisi Anne; Koehler-Santos,Patrícia; Ashton-Prolla,Patricia.
This study identifies and describes the operating costs associated with the molecular diagnosis of diseases, such as hereditary cancer. To approximate the costs associated with these tests, data informed by Standard Operating Procedures for various techniques was collected from hospital software and a survey of market prices. Costs were established for four scenarios of capacity utilization to represent the possibility of suboptimal use in research laboratories. Cost description was based on a single site. The results show that only one technique was not impacted by rising costs due to underutilized capacity. Several common techniques were considerably more expensive at 30% capacity, including polymerase chain reaction (180%), microsatellite instability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular diagnosis; Hereditary cancer; Cost analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300332

Theodosius Dobzhansky and the development of Genetics in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Pavan,Crodowaldo; Cunha,Antonio Brito da.
Tipo: Info:eu-repo/semantics/article
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300027

In silico analysis of polymorphic microsatellites in penaeid shrimp and construction of a free-access database
Provedor de dados: Genetics and Molecular Biology
Autores: Freitas,Patrícia Domingues de; Pinheiro,Allysson Pontes; Silva,Thiago Buosi; Galetti Junior,Pedro Manoel.
We performed an in silico analysis of all microsatellites so far described for penaeid shrimp and for which the polymorphic behavior has previously been analyzed. The objective of the study was to evaluate the structural characteristics of these microsatellites and identifying patterns which allow the characterization of the nature of these sequences in the penaeid genome. All data were compiled in a free-access database specially constructed for this study. Three hundred non-mononucleotide polymorphic microsatellite loci described for 12 shrimp species belonging to the family Penaeidae were analyzed and simple and compound microsatellites with di-, tri-, tetra-, penta- and hexanucleotide motifs were found. Dinucleotides and trinucleotides were the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crustacea; Microsatellite structure; Penaeidae; SSR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600027

Chromosomes of Bromus auleticus Trin. ex Nees (Poaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Martinello,Gilmar Efrem; Schifino-Wittmann,Maria Teresa.
The chromosome number of 14 accessions of Bromus auleticus Trin. ex Nees, native of Rio Grande do Sul, was 2n = 6x = 42, same ploidy level found in other South-American Bromus species. Its chromosomes were metacentric or submetacentric, ranging from ca. 4 mm to ca. 8 mm in length. Up to two satellite-bearing chromosome pairs were sometimes observed. However, as already reported for other species, the high symmetry and homogeneity of the karyotypes made it difficult to detect possible intraspecific differences.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromus auleticus; Chromosome numbers; Native forages.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300024

An excellent Brazilian book on human genetics
Provedor de dados: Genetics and Molecular Biology
Autores: Freire-Maia,Newton.
Tipo: Info:eu-repo/semantics/other
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300023

Hereditary cancer risk assessment: insights and perspectives for the Next-Generation Sequencing era
Provedor de dados: Genetics and Molecular Biology
Autores: Gomy,Israel; Diz,Maria Del Pilar Estevez.
Abstract Hereditary cancer risk assessment is a multidisciplinary and dynamic process, with the purpose of estimating probabilities of germline mutations in cancer susceptibility genes and assessing empiric risks of cancer based on personal and family histories, in order to offer clinical and molecular diagnoses and clinical management based on these risks. Genetic tests are available and most of them are reimbursed by insurance companies, although they are generally not covered by the public health systems of developing countries. More recently, molecular diagnosis of hereditary cancer is feasible through next-generation sequencing (NGS) panels. Here we review the benefits and limitations of NGS technologies in the clinical practice.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Next-generation sequencing; Hereditary cancer; Genetic counseling; Risk assessment.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200184

Responses to reciprocal recurrent selection and changes in genetic variability in IG-1 and IG-2 maize populations
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Mateus Figueiredo; Moro,Gustavo Vitti; Aguiar,Aurélio Mendes; Souza Jr.,Cláudio Lopes de.
This paper reports the effects of three cycles of reciprocal recurrent selection (RRS) on the means, genetic variances, and on the genetic correlations for several traits in the IG-1 and IG-2 maize (Zea mays L.) populations. Interpopulation full-sib progenies from cycle zero (C0) and from cycle 3 (C3) of RRS were evaluated in two locations. RRS was highly effective to improve the traits according the objectives of the program: grain yield and prolificacy increased significantly, while plant height, ear height, and ear placement decreased significantly. Genetic variances for all traits decreased significantly from C0 to C3, but the genetic correlations did not change consistently across the cycles of selection. The expected responses to the fourth cycle of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maize; Responses to selection; Genetic variances; Genetic correlations.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500021

Genetics of resistance to soybean cyst nematode, Heterodera glycines ichione (Race 3), in a brazilian soybean population
Provedor de dados: Genetics and Molecular Biology
Autores: Mauro,Antonio Orlando; Oliveira,Antonio Luiz de; Mauro,Sonia Marli Z.
A study was made of the genetics of resistance to the soybean cyst nematode, race 3, in a population derived from crosses between the Brazilian soybean genotypes BR 90-4722 and FT-Cristalina. Crosses between the two parents were made, the F1 and F2 generations were obtained and the population was analyzed for the number of cysts found in each plant of each generation as well as the type of reaction to the nematode. The results showed that resistance to the cyst nematode in this soybean population is of a qualitative nature and conditioned by three genes, one dominant and two recessives. The heritability of the character was very high (0.96), with minimum environmental effect, which means that this population is suitable for the development of soybean...
Tipo: Info:eu-repo/semantics/other
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200021

Genetic characterization of the Neotropical catfish Pimelodus maculatus (Pimelodidae, Siluriformes) in the Upper Uruguay River
Provedor de dados: Genetics and Molecular Biology
Autores: Ribolli,Josiane; Melo,Cláudio Manoel Rodrigues de; Zaniboni-Filho,Evoy.
Freshwater fish present unique challenges when one attempts to understand the factors that determine the structure of their populations. Habitat fragmentation is a leading cause of population decline that threatens ecosystems worldwide. In this study, we investigated the conservation status of genetic variability in the Neotropical catfish (Pimelodus maculatus). Specifically, we examined the structure and genetic diversity of this species in a region of the Upper Uruguay River fragmented by natural barriers and dams. There was no genetic structure among the four sites analyzed, indicating the existence of only one population group. A combination of environmental management and genetic monitoring should be used to minimize the impact of impoundment on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Dams; Habitat fragmentation; Microsatellite.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500009

DNA barcoding in Atlantic Forest plants: what is the best marker for Sapotaceae species identification?
Provedor de dados: Genetics and Molecular Biology
Autores: Vivas,Caio Vinicius; Moraes,Ramiris César Souza; Alves-Araújo,Anderson; Alves,Marccus; Mariano-Neto,Eduardo; van den Berg,Cássio; Gaiott,Fernanda Amato.
The Atlantic Forest is a phytogeographic domain with a high rate of endemism and large species diversity. The Sapotaceae is a botanical family for which species identification in the Atlantic Forest is difficult. An approach that facilitates species identification in the Sapotaceae is urgently needed because this family includes threatened species and valuable timber species. In this context, DNA barcoding could provide an important tool for identifying species in the Atlantic Forest. In this work, we evaluated four plant barcode markers (matK, rbcL, trnH-psbA and the nuclear ribosomal internal transcribed spacer region -ITS) in 80 samples from 26 species of Sapotaceae that occur in the Atlantic Forest. ITS yielded the highest average interspecific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Internal transcribed spacer; Taxonomy; Tree species; Tropical forest.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500009

Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry
Provedor de dados: Genetics and Molecular Biology
Autores: Mendes-da-Glória,Fernanda Januzzi; Mourão Filho,Francisco de Assis Alves; Camargo,Luis Eduardo Aranha; Mendes,Beatriz Madalena Januzzi.
Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck) and Rangpur lime (C. limonia L. Osbeck). The plants were confirmed as somatic hybrids by leaf morphology, chromosome number and RAPD profile. All regenerated plants were tetraploid (2n = 4x = 36), with intermediate leaf morphology and complementary RAPD banding profile of both parents. This combination may be useful as a rootstock for the citrus industry in Southeastern Brazil since this somatic hybrid could combine the drought tolerance and vigor of Rangpur lime with the blight tolerance of Caipira sweet orange.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300026

Comparison of breeding value prediction for two traits in a Nellore-Angus crossbred population using different Bayesian modeling methodologies
Provedor de dados: Genetics and Molecular Biology
Autores: Hanna,Lauren L. Hulsman; Garrick,Dorian J.; Gill,Clare A.; Herring,Andy D.; Sanders,James O.; Riley,David G.
The objectives of this study were to 1) compare four models for breeding value prediction using genomic or pedigree information and 2) evaluate the impact of fixed effects that account for family structure. Comparisons were made in a Nellore-Angus population comprising F2, F3 and half-siblings to embryo transfer F2 calves with records for overall temperament at weaning (TEMP; n = 769) and Warner-Bratzler shear force (WBSF; n = 387). After quality control, there were 34,913 whole genome SNP markers remaining. Bayesian methods employed were BayesB ( π = 0.995 or 0.997 for WBSF or TEMP, respectively) and BayesC (π = 0 and π), where π is the ideal proportion of markers not included. Direct genomic values (DGV) from single trait Bayesian analyses were compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bayesian inference; Crossbred cattle; Genomic prediction; Model comparison.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500005

First report of a B chromosome in a natural population of Astyanax altiparanae (Characiformes, Characidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Hashimoto,Diogo Teruo; Gonçalves,Vanessa Regina; Bortolozzi,Jehud; Foresti,Fausto; Porto-Foresti,Fábio.
Several species of the genus Astyanax have already been genetically studied, and B-chromosomes have been considered to be an interesting feature in some species of this group. In the present paper we report, for the first time, the occurrence of a B microchromosome in a natural population of A. altiparanae. This additional genomic element was identified as an acrocentric chromosome, similar in size to the smallest chromosomal pairs of the standard karyotype. Analysis of the constitutive heterochromatin pattern by C-banding evidenced heterochromatic blocks located on centromeric, pericentromeric, and interstitial regions of some chromosomes, and also positive marks in a subtelocentric chromosomal pair that presented the short arms entirely heterochromatic....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B chromosomes; Fish cytogenetics; Astyanax altiparanae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200021

Lack of association between parenchymal neurocysticercosis and HLA Class I and Class II antigens
Provedor de dados: Genetics and Molecular Biology
Autores: Bompeixe,Eni Picchioni; Costa,Sonia Maria Correia Machado da; Arruda,Walter Oleschko; Petzl-Erler,Maria Luiza.
Neurocysticercosis, caused by encysted larvae of the tapeworm Taenia solium, is the most common infection of the central nervous system and a major public health problem in many countries. Prevalence in the region of Curitiba, located in the southern Brazilian State of Paraná, is one of the highest in the world. The genetics of host susceptibility to neurocysticercosis (NCC) is still obscure. To investigate if major histocompatibility complex (MHC) genes influence individual susceptibility to NCC, we performed a case-control association analysis. Fifty-two Caucasoid patients and 149 matched controls were typed for antigens of the HLA-A, B, C, DR and DQ loci. All patients had computerized tomography and clinical features compatible with parenchymal NCC....
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100002

Fixed bin frequency distribution for the VNTR Loci D2S44, D4S139, D5S110, and D8S358 in a population sample from Minas Gerais, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Parreira,Kleber Simônio; Vieira,Gismar Silva; Inácio,Juarez; Moura-Neto,Rodrigo Soares; Goulart,Luiz Ricardo.
Fixed bin frequencies for the VNTR loci D2S44, D4S139, D5S110, and D8S358 were determined in a Minas Gerais population sample. The data were generated by RFLP analysis of HaeIII-digested genomic DNA and chemiluminescent detection. The four VNTR loci have met Hardy-Weinberg equilibrium, and there was no association of alleles among VNTR loci. The frequency data can be used in forensic analyses and paternity tests to estimate the frequency of a DNA profile in the general Brazilian population.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fixed-bin frequency; VNTR loci; Brazilian population.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300004

Demographic history of wood stork (Mycteria americana) Brazilian Pantanal colonies revealed by mitochondrial DNA analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Lopes,Iara Freitas; Brito,Reinaldo Alves de; Henrique-Silva,Flávio; Del Lama,Silvia Nassif.
We used mitochondrial DNA (mtDNA) sequences to investigate the demographic history of the wood stork (Mycteria americana) populations in the Brazilian Pantanal. Sequences of 390/460 bp fragment of the mtDNA control region were analyzed in 62 wood stork specimens from 8 colonies using neutrality tests, phylogeographic, and coalescent analyses. Population expansion was supported by the significantly negative values of Tajima’s (D = -2.071) and Fu’s (Fs = -14.544) statistics and the unimodal pattern of mismatch distribution. Nested clade analyses indicated a historic range expansion event and recurrent gene flow that was restricted by isolation by distance as explanations for the haplotype distribution among the sampled colonies. High genetic diversity and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wood stork; Mycteria americana; Pantanal; Mitochondrial DNA; Phylogeography.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200008

DNA repair-related genes in sugarcane expressed sequence tags (ESTs)
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,R.M.A.; Lima,W.C.; Vogel,C.I.G.; Berra,C.M.; Luche,D.D.; Medina-Silva,R.; Galhardo,R.S.; Menck,C.F.M.; Oliveira,V.R.
There is much interest in the identification and characterization of genes involved in DNA repair because of their importance in the maintenance of the genome integrity. The high level of conservation of DNA repair genes means that these genetic elements may be used in phylogenetic studies as a source of information on the genetic origin and evolution of species. The mechanisms by which damaged DNA is repaired are well understood in bacteria, yeast and mammals, but much remains to be learned as regards plants. We identified genes involved in DNA repair mechanisms in sugarcane using a similarity search of the Brazilian Sugarcane Expressed Sequence Tag (SUCEST) database against known sequences deposited in other public databases (National Center of...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100018

MTHFR , prothrombin and Factor V gene variants in Turkish patients   with coronary artery stenosis
Provedor de dados: Genetics and Molecular Biology
Autores: Caner,Müge; Bircan,Rifat; Sevinç,Deniz; Benli,Fehime; Güney,A. Ilter; Kurtoglu,Nuri.
Many epidemiological studies have reported an association between hemostatic factors and risk of both coronary and peripheral artery diseases. Using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis, we investigated the association between coronary artery disease and polymorphisms in the methylenetetrahydrofolate reductase ( MTHFR  C677T and A1298C), prothrombin (G20210A), and factor V (A4070G) genes. We screened these gene variants in 174 subjects who had undergone coronary angiography - 115 patients with patent coronary artery disease (grade 3 vessel disease,  i.e. , significant coronary stenosis), and 59 healthy controls with grade 0 vessel disease. The analysis of our data did not show any statistically...
Palavras-chave: Genetic polymorphism; Coronary disease; MTHFR gene; Prothrombin gene; Factor V gene.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500006

A novel point mutation in a class IV glucose-6-phosphate dehydrogenase variant (G6PD São Paulo) and polymorphic G6PD variants in São Paulo State, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Raimundo Antonio G.; Oshiro,Marilena; Hirata,Mario H.; Hirata,Rosario D.C.; Ribeiro,Georgina S.; Medeiros,Tereza M.D.; Barretto,Orlando C. de O.
In this study, we used red cell glucose-6-phosphate dehydrogenase (G6PD) activity to screen for G6PD-deficient individuals in 373 unrelated asymptomatic adult men who were working with insecticides (organophosphorus and carbamate) in dengue prevention programs in 27 cities in São Paulo State, Brazil. Twenty-one unrelated male children suspected of having erythroenzymopathy who were attended at hospitals in São Paulo city were also studied. Fifteen of the 373 adults and 12 of the 21 children were G6PD deficient. G6PD gene mutations were investigated in these G6PD-deficient individuals by using PCR-RFLP, PCR-SSCP analysis and DNA sequencing. Twelve G6PD A-202A/376G and two G6PD Seattle844C, as well as a new variant identified as G6PD São Paulo, were detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucose-6-phosphate dehydrogenase; Mutations; Polymorphism; Variants.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200007

Interactions between plant hormones and heavy metals responses
Provedor de dados: Genetics and Molecular Biology
Autores: Bücker-Neto,Lauro; Paiva,Ana Luiza Sobral; Machado,Ronei Dorneles; Arenhart,Rafael Augusto; Margis-Pinheiro,Marcia.
Abstract Heavy metals are natural non-biodegradable constituents of the Earth's crust that accumulate and persist indefinitely in the ecosystem as a result of human activities. Since the industrial revolution, the concentration of cadmium, arsenic, lead, mercury and zinc, amongst others, have increasingly contaminated soil and water resources, leading to significant yield losses in plants. These issues have become an important concern of scientific interest. Understanding the molecular and physiological responses of plants to heavy metal stress is critical in order to maximize their productivity. Recent research has extended our view of how plant hormones can regulate and integrate growth responses to various environmental cues in order to sustain life. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ABA; Auxin; Brassinosteroid; Ethylene; Abiotic stress.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200373

Genetic structure analysis of Eufriesea violacea (Hymenoptera, Apidae) populations from southern Brazilian Atlantic rainforest remnants
Provedor de dados: Genetics and Molecular Biology
Autores: Sofia,Silvia H.; Paula,Francine M. de; Santos,Aline M. dos; Almeida,Fernanda S.; Sodré,Leda M.K.
Random amplified polymorphic DNA (RAPD) markers were used to analyze the genetic structure of Eufriesea violacea populations in three fragments (85.47, 832.58 and 2800 ha) of Atlantic rainforest located in the north of the Brazilian state of Paraná. A total of twelve primers produced 206 loci, of which 129 were polymorphic (95% criterion). The proportions of polymorphic loci in each population ranged from 57.28% to 59.2%, revealing very similar levels of genetic variability in the groups of bees from each fragment. Unbiased genetic distances between groups ranged from 0.0171 to 0.0284, the smallest genetic distance occurring between bees from the two larger fragments. These results suggest that the E. violacea populations from the three fragments have...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Euglossine bees; Euglossina; Rainforest fragments; Genetic variability; RAPD.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300026

Unravelling MADS-box gene family in Eucalyptus spp.: a starting point to an understanding of their developmental role in trees
Provedor de dados: Genetics and Molecular Biology
Autores: Dias,Beatriz Fonseca de Oliveira; Simões-Araújo,Jean Luiz; Russo,Claudia A.M.; Margis,Rogério; Alves-Ferreira,Márcio.
MADS-box genes encode a family of transcription factors which control diverse developmental processes in flowering plants ranging from root to flower and fruit development. Members of the MADS-box gene family share a highly conserved sequence of approximately 180 nucleotides that encodes a DNA-binding domain. We used bioinformatics tools to investigate the information generated by the Eucalyptus Expressed Sequence Tag (FORESTs) genome project in order to identify and annotate MADS-box genes. The comparative phylogenetic analysis of the Eucalyptus MADS-box genes with Arabidopsis homologues allowed us to group them into one of the well-known subfamilies. Trends in gene expression of these putative Eucalyptus MADS-box genes were investigated by hierarchical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MADS-box; Vegetative development; Reproductive development; Phylogenetic analysis; ESTs.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400004

Clonal diversity and conservation genetics of the medicinal plant Carapichea ipecacuanha (Rubiaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Luiz Orlando de; Venturini,Bruna Afonso; Rossi,Ana Aparecida Bandini; Hastenreiter,Saulo Santos.
The roots of the understorey shrub Carapichea ipecacuanha (ipecac) have medicinal properties, and the uprooting of wild plants has supplied most of the world demand for this species. Although under severe population decline, C. ipecacuanha lacks legal protection. In the wild, the aerial stems of ipecac clump together to form clusters with well-defined borders. Cluster size may range from several to hundreds of aerial stems. To investigate the extent of clonality among aerial stems in ipecac clusters, we sampled 50 wild clusters (a total of 291 aerial stems) and screened them with 89 inter-simple sequence repeat (ISSR) markers. The 291 aerial stems were grouped into 42 putative clones. The clonal groups generally consisted of aerial stems from the same...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clonal propagation; Genetic diversity; ISSR; Poaia; Psychotria ipecacuanha.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100016

Distribution of DNA repair-related ESTs in sugarcane
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,W.C.; Medina-Silva,R.; Galhardo,R.S.; Menck,C.F.M.
DNA repair pathways are necessary to maintain the proper genomic stability and ensure the survival of the organism, protecting it against the damaging effects of endogenous and exogenous agents. In this work, we made an analysis of the expression patterns of DNA repair-related genes in sugarcane, by determining the EST (expressed sequence tags) distribution in the different cDNA libraries of the SUCEST transcriptome project. Three different pathways - photoreactivation, base excision repair and nucleotide excision repair - were investigated by employing known DNA repair proteins as probes to identify homologous ESTs in sugarcane, by means of computer similarity search. The results showed that DNA repair genes may have differential expressions in tissues,...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100019

A re-annotation of the Anopheles darlingi mobilome
Provedor de dados: Genetics and Molecular Biology
Autores: Diesel,Jose Francisco; Ortiz,Mauro F.; Marinotti,Osvaldo; Vasconcelos,Ana Tereza R.; Loreto,Elgion L. S.
Abstract The mobilome, portion of the genome composed of transposable elements (TEs), of Anopheles darlingi was described together with the genome of this species. Here, this mobilome was revised using similarity and de novo search approaches. A total of 5.6% of the A. darlingi genome is derived of TEs. Class I gypsy and copia were the most abundant superfamilies, corresponding to 22.36% of the mobilome. Non-LTR elements of the R1 and Jockey superfamilies account for 11% of the TEs. Among Class II TEs, the mariner superfamily is the most abundant (16.01%). Approximately 87% of the A. darlingi mobilome consist of short, truncated and/or degenerated copies of TEs. Only three retrotransposons, two belonging to gypsy and one to copia superfamilies, are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transposable elements; Transposons; Mosquitoes; Evolvability.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100125

Karyotypes of Brazilian non-volant small mammals (Didelphidae and Rodentia): An online tool for accessing the chromosomal diversity
Provedor de dados: Genetics and Molecular Biology
Autores: Paresque,Roberta; Rodrigues,Jocilene da Silva; Righetti,Kelli Beltrame.
Abstract We have created a database system named CIPEMAB (CItogenética dos PEquenos MAmíferos Brasileiros) to assemble images of the chromosomes of Brazilian small mammals (Rodents and Marsupials). It includes karyotype information, such as diploid number, karyotype features, idiograms, and sexual chromosomes characteristics. CIPEMAB facilitates quick sharing of information on chromosome research among cytogeneticists as well as researchers in other fields. The database contains more than 300 microscopic images, including karyotypic images obtained from 182 species of small mammals from the literature. Researchers can browse the contents of the database online (http://www.citogenetica.ufes.br). The system enables users to locate images of interest by taxa,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype diversity; Cytogenetic; Cytogenetic database.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400605

Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Mayra Ramos de Jesus; Pinhatti,Valéria Rodrigues; Silveira,Maiele Dornelles da; Matzenbacher,Cristina Araujo; Freitas,Thales Renato Ochotorena de; Silva,Juliana da; Camassola,Melissa; Nardi,Nance Beyer.
Abstract Mesenchymal stem/stromal cells (MSCs) are multipotent cells distributed in all tissues and characterized by adherence, morphology, immunophenotype and trilineage differentiation potential. The present study aimed to isolate and characterize adherent MSC-like populations from different tissues of Ctenomys minutus, a threatened wildlife rodent popularly known as tuco-tuco. Adherent cells were isolated from bone marrow, brain, liver, pancreas and adipose tissue of three adult animals collect in southern Brazil. Cultures showed typical morphology and proliferation potential. Adipose-derived MSCs showed trilineage potential. Cultures derived from adipose tissue, bone marrow and brain were immunophenotyped with negative results for CD31, CD44, CD45,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem/stromal cells; Tuco-tuco; Ctenomys minutus.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500870

Distribution and evolution of secondary metabolites in Eriocaulaceae, Lythraceae and Velloziaceae from "campos rupestres"
Provedor de dados: Genetics and Molecular Biology
Autores: Salatino,Antonio; Salatino,Maria Luiza Faria; Santos,Déborah Yara A.C. dos; Patrício,Márcia Cristina B.
Hypotheses are presented on the evolution of structural patterns of secondary metabolites (flavonoids and foliar wax alkanes) and fatty acids of families of "campos rupestres". The distribution of fatty acids is given for genera of Lythraceae, with emphasis on Cuphea (supposedly more advanced) and Diplusodon. Compounds with saturated short chains represent a derived condition in Lythraceae although they are probably restricted to Cuphea. It is suggested that evolution selected for more complex flavonoid patterns in Cuphea, with the inclusion of C-glycoflavones and methoxylated flavonols (rhamnetin and isorhamnetin), which are not found in members of Diplusodon and Lafoensia. The supposedly primitive groups of Eriocaulaceae (e.g., Paepalanthus) presented...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400038

A parallel algorithm for finding small sets of genes that are enough to distinguish two biological states
Provedor de dados: Genetics and Molecular Biology
Autores: Torres,Martha; Barrera,Junior.
GCLASS is an algorithm which explores small samples of two distinct biological states for finding small sets of genes, which form a feature vector that is enough to separate these two states. A typical sample is a set of 60 microarrays, 30 for each biological state, with several thousand genes. The technique consists of the following: a spreading model defined in the space of small sets of genes studied and centered in each feature vector considered; the designing of optimal linear classifiers under this spreading model; and ranking the designed classifiers, based on their error and robustness relative to the spreading. The feature vectors used in the best classifiers are considered the best feature vectors. Due to the great number of potential feature...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; Classification; Parallel processing.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400034

In silico analysis of Eucalyptus thioredoxins
Provedor de dados: Genetics and Molecular Biology
Autores: Barbosa,Aulus Estevão; Marinho,Paulo.
The Eucalyptus Genome Sequencing Project (FORESTs), an initiative from the Brazilian ONSA consortium (Organization for Nucleotide Sequencing and Analysis), has achieved the sequencing of 123.889 EST clones from 18 different cDNA libraries. We have investigated the FORESTs data set to identify EST clusters potentially encoding thioredoxins (TRX). Two types of thioredoxin families described in plants, chloroplastic (TRXm/f/x/y) and cytosolic (TRXh), have been found in the transcriptome. Putative typical TRXs have been identified in fifteen clusters, four m-type, seven h-type, two f-type, one cluster for each x/y-types and one putative homologue of the TDX gene from Arabidopsis thaliana. One cluster presents an atypical active site WCMPS, different from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus; Transcriptome; Thioredoxin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400008

Enzymatic variability in Aedes aegypti (Diptera: Culicidae) populations from Manaus-AM, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Fraga,Elmary da Costa; Santos,Joselita Maria Mendes dos; Maia,Juracy de Freitas.
Eighteen enzymatic loci were analysed in Aedes aegypti populations from four neighbourhoods in the city of Manaus. The analyses showed that the Downtown population was the most polymorphic (p = 55.6%) with higher observed and expected mean heterozygosities (Ho = 0.152 ± 0.052; He = 0.174 ± 0.052, respectively). The least variability was detected in the Coroado and Cidade Nova populations, both with polymorphism of 44.4%. The latter population presented the least observed heterozygosity (Ho = 0.109 ± 0.037). Wright's F statistics showed that the mean value of Fis was higher than that of Fst (Fis = 0.164 &gt; Fst = 0.048), and from analysis of molecular variance (AMOVA) it was found that 95.12% of the variability is found within populations indicating a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aedes aegypti; Electrophoreses; Isozymes; Populations genetics.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200011

Chromosomal diversity in three species of electric fish (Apteronotidae, Gymnotiformes) from the Amazon Basin
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Fernando Henrique Ramos; Pieczarka,Julio Cesar; Cardoso,Adauto Lima; Silva,Patrícia Corrêa da; Oliveira,Jonas Alves de; Nagamachi,Cleusa Yoshiko.
Cytogenetic studies were carried out on samples of Parapteronotus hasemani, Sternarchogiton preto and Sternarchorhamphus muelleri (Apteronotidae, Gymnotiformes) from the Amazon basin. The first two species exhibited both a 2n = 52 karyotype, but differed in their karyotypic formulae, distribution of constitutive heterochromatin, and chromosomal location of the NOR. The third species, Sternarchorhamphus muelleri, was found to have a 2n = 32 karyotype. In all three species the DAPI and chromomycin A3 staining results were consistent with the C-banding results and nucleolar organizer region (NOR) localization. The 18S rDNA probe confirmed that there was only one pair of ribosomal DNA cistron bearers per species. The telomeric probe did not reveal interstitial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluorochromes; FISH; Chromosomal rearrangements; Biodiversity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500006

Allozyme variation among three populations of the armored catfish Hypostomus regani (Ihering, 1905) (Siluriformes, Loricariidae) from the Paraná and Paraguay river basins, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Zawadzki,Cláudio H.; Renesto,Erasmo; Peres,Maria Dolores; Paiva,Suzana.
Three Brazilian populations of the armored catfish Hypostomus regani (Ihering, 1905) were sampled, one from the Corumbá Reservoir in Goiás state, another from the Itaipu Reservoir in Paraná state and a third from the Manso Reservoir in Mato Grosso state. Allozyme electrophoresis was used to establish the genetic structure of the species, with the analysis of liver, heart and muscles tissues allowing the scoring of 25 loci from 14 enzymatic systems. Although no diagnostic loci were found, some exclusive rare alleles were recorded for the three populations. The genetically most similar populations were those from Corumbá and Itaipu, and the most distant were the populations from Manso and Corumbá. The allozyme data showed three structured populations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allozymes; Genetic variability; Hypostomus regani; Loricariidae; Paraguay and Paraná Rivers.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400025

PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze
Provedor de dados: Genetics and Molecular Biology
Autores: Schlögl,Paulo Sérgio; Souza,Anete Pereira de; Nodari,Rubens Onofre.
The Araucaria angustifolia (Bert.) O. Kuntze, also named the "paraná pine" (‘pinheiro-do-Paraná’ in Portuguese), is a native conifer species naturally occurring in the Brazilian Tropical Atlantic Forest which in Brazil is mostly limited to the southern Brazilian states of Paraná, Santa Catarina and Rio Grande do Sul. Chloroplast DNA markers (cpDNA) are useful in populational genetic studies because of their low substitution rate and the uniparental transmission. The conservation of cpDNA genes between species has allowed the design of consensus chloroplast primers that have had a great impact on population genetics and phylogenetic studies. In this study we used the polymerase chain reaction technique combined with restriction enzyme fragment length...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haplotypes; Conifer species; Brazilian pine; Genetic diversity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300020

Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci
Provedor de dados: Genetics and Molecular Biology
Autores: Mantellatto,Aline Meira Bonfim; Caparroz,Renato; Christofoletti,Maurício Durante; Piovezan,Ubiratan; Duarte,José Maurício Barbanti.
Abstract The pampas deer (Ozotoceros bezoarticus) is close to being classified as ‘globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Fecal DNA; Microsatellites; Non-invasive methods; Pampas deer.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500774

The pequi pulp oil (Caryocar brasiliense Camb.) provides protection against aging-related anemia, inflammation and oxidative stress in Swiss mice, especially in females
Provedor de dados: Genetics and Molecular Biology
Autores: Roll,Mariana Matos; Miranda-Vilela,Ana Luisa; Longo,João Paulo Figueiró; Agostini-Costa,Tania da Silveira; Grisolia,Cesar Koppe.
Abstract Continued exposure to reactive oxygen species and inflammation are the rationale behind aging theories and associated diseases. Scientific evidence corroborates the ethnomedicinal use of the oil of pequi (Caryocar brasiliense Camb.), a typical Brazilian Cerrado fruit, against oxidative damage to biomolecules and inflammation. We aimed to investigate in vivo the antioxidant and anti-inflammatory effects of pequi oil on hemogram and DNA damage in healthy young adult and older middle-aged Swiss mice of both genders. Animals, aged 6-7 and 11-12 months, were orally treated for 15 days with pequi oil at 30 mg/day. Blood samples were used for hemogram and comet assay, and bone marrow for micronucleus test. Female controls of 11-12 months had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging-related inflammation and oxidative stress; Comet assay; Micronucleus; Antioxidant; Folk medicine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500858

Three Mexican Families with β thalassemia intermedia with different molecular basis
Provedor de dados: Genetics and Molecular Biology
Autores: Torre,Lourdes del Carmen Rizo de la; Díaz,Francisco Javier Perea; Cortés,Bertha Ibarra; López,Víctor Manuel Rentería; López,Josefina Yoaly Sánchez; Anzaldo,Francisco Javier Sánchez; Torres,María Teresa Magaña; Gonnet,Katia; Badens,Catherine; Bonello-Palot,Nathalie.
Abstract Beta thalassemia (β-thal) is a frequent monogenic disease, is clinically and molecularly heterogeneous. This study described molecular and laboratory findings for three Mexican patients with β-thal intermedia phenotype and their relatives. Three Mexican families were studied for presenting β-thal intermedia, ARMS-PCR and Gap-PCR were performed to screen for common mutations, Sanger sequencing for rare or new alleles, and MLPA for identifying deletions and or duplications. In all three families we observed, in heterozygote condition, the mutation c.118C &gt; T (p.Gln39*) also known as codon 39(C &gt; T) in the β globin gene (HBB) associated with a novel molecular defect: a new duplication of the alpha globin gene cluster, a new deletion that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thalassemia intermedia; Mexican population; Β globin gene; New mutations; Alpha-globin gene duplication.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500104

Interchromosomal distribution of gamma ray-induced chromatid aberrations in Chinese hamster ovary cells
Provedor de dados: Genetics and Molecular Biology
Autores: Martínez-López,Wilner; Porro,Valentina; Folle,Gustavo A.; Mendez-Acuña,Leticia; Savage,John R.K.; Obe,Günter.
Interchromosomal distributions of breakpoints from chromatid-type aberrations induced by gamma rays in Chinese hamster ovary (CHO) cells were analyzed. In most chromosomes the distribution was as expected from chromosome lengths for simple breaks or the respective relative corrected length in case of exchanges. There were deviations from expectation in a few chromosomes for chromatid breaks, interchanges, intra-arm intrachanges and inter-arm intrachanges. Especially interesting are the results concerning chromosomes 2 and 8, which were more often involved in exchanges than expected. An "exchange phenotype" for these chromosomes is proposed and possible explanations for the nonrandom distribution of chromosome breakpoints are presented.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400053

Antigenotoxicity and antimutagenicity of ethanolic extracts of Brazilian green propolis and its main botanical source determined by the Allium cepa test system
Provedor de dados: Genetics and Molecular Biology
Autores: Roberto,Matheus Mantuanelli; Jamal,Cláudia Masrouah; Malaspina,Osmar; Marin-Morales,Maria Aparecida.
Abstract Brazilian green propolis is a resinous substance prepared by bees from parts of the plant Baccharis dracunculifolia. As it possess several biological properties, this work assessed the cytotoxic/anticytotoxic, genotoxic/antigenotoxic and mutagenic/antimutagenic potential of ethanolic extracts of Brazilian green propolis (EEGP) and of B. dracunculifolia (EEBD), by means of the Allium cepa test system. The effects were evaluated by assessing the chromosomal aberrations (CA) and micronuclei (MN) frequencies on meristematic and F1 generation cells from onion roots. Chemical analyses performed with the extracts showed differences in flavonoid quality and quantity. No genotoxic or mutagenic potential was detected, and both extracts were capable of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal aberration; Micronucleus; Anticlastogenicity; Protective effects; Flavonoids.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200257

Influence of obesity gene in quantitative traits of swine
Provedor de dados: Genetics and Molecular Biology
Autores: Borges,Graciele Segantini do Nascimento; Goulart,Luiz Ricardo.
Genotype data of 477 animals of several swine races (Landrace - LD, Large White - LW, Pietrain - PI, LWXLDXPI, Piau, Monteiro, and unknown race) were obtained to determine the allele frequency of the obesity gene. Genotype data of 174 crossbred swine (LWXLDXPI) were also obtained, in order to assess its correlation with carcass evaluation data (lean meat percentage, backfat thickness at P2, loin eye area, adjacent fat area, total fat and meat). Finally, genotype data of 96 pure swine (Landrace, Large White and Pietrain) were collected, to establish its relation with meat quality (drip loss, meat color, texture analysis and intramuscular fat) and carcass evaluation data (lean meat percentage; ham, loin, shoulder and belly weights; and backfat thickness at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obese gene; Leptin gene; Quantitative traits.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100007

Premature ovarian failure (POF) in Brazilian fragile X carriers
Provedor de dados: Genetics and Molecular Biology
Autores: Vianna-Morgante,Angela M.; Costa,Silvia S.; Pavanello,Rita de C.M.; Otto,P.A.; Mingroni-Netto,Regina C.
The gynecological and reproductive histories of 193 women from fragile X families were surveyed. Among the 101 carriers of the premutation, 14 experienced premature menopause, contrarily to their 37 fully mutated and 55 noncarrier female relatives. Although premature menopause showed a tendency to cluster in certain fragile X families, as a group, the premutated women experienced menopause earlier than noncarriers. This suggests that premature menopause may be the extreme effect of a spectrum of ovarian anomalies associated with the fragile X premutation.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400002

Chromosomal diversification of diploid number, heterochromatin and rDNAs in two species of Phanaeus beetles (Scarabaeidae, Scarabaeinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Arcanjo,Amanda; Cabral-de-Mello,Diogo Cavalcanti; Martins,Cesar; Moura,Rita de Cássia de; Souza,Maria José de.
The genus Phanaeus is included in the tribe Phanaeini, one of the most diverse tribes within the subfamily Scarabaeinae in terms of chromosomal characteristics. However, so far the species of this genus were not studied with differential cytogenetic techniques, limiting any inference of the probable mechanisms responsible for this diversity. In this work, several techniques were applied with the aim of cytogenetically characterizing two Phanaeus species. The karyotype found for Phanaeus (Notiophanaeus) chalcomelas was 2n = 12, neo-XY, and that of P. (N.) splendidulus was 2n = 20, Xy p, considered primitive for the family Scarabaeidae. The chromosomes of both species showed a high amount of constitutive heterochromatin (CH), with blocks rich in base pairs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Chromosome evolution; Repetitive DNA; FISH.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300007

Association of the bovine major histocompatibility complex (BoLA) BoLA-DRB3 gene with fat and protein production and somatic cell score in Brazilian Gyr dairy cattle (Bos indicus)
Provedor de dados: Genetics and Molecular Biology
Autores: Nascimento,Carlos Souza do; Machado,Marco Antonio; Martinez,Mario Luiz; Silva,Marcos Vinícius G. Barbosa da; Guimarães,Marta Fonseca Martins; Campos,Ana Lúcia; Azevedo,Ana Luisa Sousa; Teodoro,Roberto Luiz; Verneque,Rui da Silva; Guimarães,Simone Eliza Facioni; Oliveira,Denise Aparecida Andrade.
The effect of the bovine major histocompatibility complex (BoLA) locus on animal health may be due to a direct action of its alleles on immune functions, whereas its indirect effect on production traits might be explained by the better general health conditions of more productive animals. In the present study, the BoLA-DRB3 gene was investigated in 1058 cows belonging to seven Brazilian Gyr Dairy herds (Bos indicus, Zebu cattle). A total of 37 alleles were identified, 15 of them described for the first time in a Zebu breed. A highly significant association (p &lt; 0.02) was observed between allele *54 and a decrease (-26.1 kg) in milk protein yield and there was a significant association (p &lt; 0.05) between this allele and lower (-26.07 kg) milk fat...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Marker-assisted selection; Molecular markers; PCR-RFLP.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400011

Comparative transcriptome analysis of the hippocampus from sleep-deprived and Alzheimer’s disease mice
Provedor de dados: Genetics and Molecular Biology
Autores: Wei,Yi.
Abstract We did a comparative analysis of the gene expression profiles of the hippocampus from sleep deprivation and Alzheimer’s disease (AD) mice. Differentially expressed genes (DEGs) were identified by comparing the transcriptome profiles of the hippocampus of sleep deprivation or AD mouse models to matched controls. The common DEGs between sleep deprivation and AD were identified by the overlapping analysis, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The results showed that a total of 16 common DEGs showed similar change patterns in both sleep deprivation mice and AD mice. Sgk1, Ly6a, Atp6v0e, Hspb8, Htra1, Pdk4, Pfkfb3, Golm1, and Plin3 were up-regulated in the two disorders, whereas, Marcksl1, Fgd1,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep deprivation; Alzheimer’s disease; Hippocampus; Acetyl-CoA; Lipid.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400703

Obesity-related gene ADRB2, ADRB3 and GHRL polymorphisms and the response to a weight loss diet intervention in adult women
Provedor de dados: Genetics and Molecular Biology
Autores: Saliba,Louise F.; Reis,Rodrigo S.; Brownson,Ross C.; Hino,Adriano A.; Tureck,Luciane V.; Valko,Cheryl; Souza,Ricardo L.R. de; Furtado-Alle,Lupe.
The individual response to diet may be influenced by gene polymorphisms. This study hypothesized that ADRB2 (Gln27Glu, rs1042714 and Arg16Gly, rs1042713), ADRB3 (Trp64Arg, rs4994) and GHRL (Leu72Met, rs696217) polymorphisms moderate weight loss. The study was a seven weeks dietary weight loss intervention with Brazilian adult obese women (n = 109). The body mass index (BMI) was calculated and polymorphisms in these genes were assessed by real-time PCR assays. Two-way repeated-measures ANOVA (2 x 2) were used to analyze the intervention effect between polymorphisms and BMI over the period and after stratification for age and socioeconomic status (SES). The weight loss intervention resulted in decreased BMI over the seven-week period (p &lt; 0.001), for high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Weight loss; Adrenergic receptor polymorphism; Ghrelin polymorphism; Nutrigenetics.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100005

Genetic causes involved in Leishmania Chagasi infection innortheastern: Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Feitosa,Mary Furlan; Azevêdo,Eliane; Lima,Ângela Maria; Krieger,Henrique.
A sample of 502 individuals from 94 families from Jacobina, State of Bahia, Brazil, was investigated to determine the causal mechanisms involved in Leishmania chagasi (the causal agent of visceral leishmaniasis in the American hemisphere) infection, as measured by the intradermic reaction to antigens derived from this parasite, using complex segregation analyses. The results showed evidence of a major genetic mechanism acting on infection, with a frequency of a recessive (or additive) susceptibility gene (q) of approximately 0.45. A small multifactorial component (H = 0.29) acting in conjunction with a major recessive gene (q = 0.37) is not ruled out as a concomitant causative factor.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100001

Three novel polymorphic microsatellite markers for the glaucoma locus GLC1B by datamining tetranucleotide repeats on chromosome 2p12-q12
Provedor de dados: Genetics and Molecular Biology
Autores: Murga-Zamalloa,Carlos; Guevara-Fujita,Maria Luisa; Estrada-Cuzcano,Alejandro; Fujita,Ricardo.
In order to identify new markers around the glaucoma locus GLC1B as a tool to refine its critical region at 2p11.2-2q11.2, we searched the critical region sequence obtained from the UCSC database for tetranucleotide (GATA)n and (GTCT)n repeats of at least 10 units in length. Three out of four potential microsatellite loci were found to be polymorphic, heterozygosity ranging from 64.56% to 79.59%. The identified markers are useful not only for GLC1B locus but also for the study of other disease loci at 2p11.2-2q11.2, a region with scarcity of microsatellite markers.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GLC1B; Microsatellite polymorphic markers; Tetranucleotide tandem repeat; Gene mapping; Glaucoma.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400009

Phylogeny of the Serrasalmidae (Characiformes) based on mitochondrial DNA sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Ortí,Guillermo; Sivasundar,Arjun; Dietz,Kelly; Jégu,Michel.
Previous studies based on DNA sequences of mitochondrial (mt) rRNA genes showed three main groups within the subfamily Serrasalminae: (1) a "pacu" clade of herbivores (Colossoma, Mylossoma, Piaractus); (2) the "Myleus" clade (Myleus, Mylesinus, Tometes, Ossubtus); and (3) the "piranha" clade (Serrasalmus, Pygocentrus, Pygopristis, Pristobrycon, Catoprion, Metynnis). The genus Acnodon was placed as the sister taxon of clade (2+3). However, poor resolution within each clade was obtained due to low levels of variation among rRNA gene sequences. Complete sequences of the hypervariable mtDNA control region for a total of 45 taxa, and additional sequences of 12S and 16S rRNA from a total of 74 taxa representing all genera in the family are now presented to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piranhas; Pacus; D-loop; Phylogeny; Bayesian inference.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200030

RELAGH - the challenge of having a scientific network in Latin America: an account from the presidents
Provedor de dados: Genetics and Molecular Biology
Autores: Rojas-Martínez,Augusto; Giraldo-Ríos,Alejandro; Jiménez-Arce,Gerardo; Falcón de Vargas,Aída; Giugliani,Roberto.
Latin America and the Caribbean region make up one of the largest areas of the world, and this region is characterized by a complex mixture of ethnic groups sharing Iberian languages. The area is comprised of nations and regions with different levels of social development. This region has experienced historical advances in the last decades to increase the minimal standards of quality of life; however, several factors, such as concentrated populations in large urban centers and isolated and poor communities, still have an important impact on medical services, particularly genetics services. Latin American researchers have greatly contributed to the development of human genetics and historic inter-ethnic diversity, and the multiplicity of geographic areas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Latin America and the Caribbean; Human and medical genetics; International organization; Research cooperation; Education.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200017

Characterization of ribosomal DNA (rDNA) in Drosophila arizonae
Provedor de dados: Genetics and Molecular Biology
Autores: Javier Tovar,Francisco; Silva,Luiz Antônio Ferreira da; Rodarte,Renato Santos; Leoncini,Orilio.
Ribosomal DNA (rDNA) is a multigenic family composed of one or more clusters of repeating units (RU). Each unit consists of highly conserved sequences codifying 18S, 5.8S and 28S rRNA genes intercalated with poorly conserved regulatory sequences between species. In this work, we analyzed the rDNA of Drosophila arizonae, a member of the mulleri complex (Repleta group). Using genomic restriction patterns, cloning and mapping of some representative rDNA fragments, we were able to construct a representative restriction map. RU in this species are 13.5-14 kb long, restriction sites are completely conserved compared with other drosophilids and the rDNA has an R1 retrotransposable element in some RU. We were unable to detect R2 elements in this species.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200015

Heterochromatin of maize chromosomes: structure and genetic effects
Provedor de dados: Genetics and Molecular Biology
Autores: Aguiar-Perecin,Margarida L.R. de; Fluminhan,Antonio; Santos-Serejo,Janay A. dos; Gardingo,José R.; Bertão,Mônica R.; Decico,Maria Juliana U.; Mondin,Mateus.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400047

Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain
Provedor de dados: Genetics and Molecular Biology
Autores: Gong,Jie; Xie,Yinjie; Yu,Kun; Yang,Ya'nan; Huang,Huiyang; Ye,Haihui.
Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequence and encoded a peptide of 270 amino acids with a signal peptide of 17 amino acids. The SpCHY gene contains seven exons, six introns, a TATA box and several transcription factor binding sites that were found in 5'-promoter region which is 1221 bp in length-time quantitative PCR analysis indicated that the expression level of SpCHY mRNA in hepatopancreas was significantly higher than that in other tissues. Immunocytochemistry and in situ hybridization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chymotrypsin; Scylla paramamosain; Immune response; Immunocytochemistry; In situ hybridization.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300010

Transcript levels of ten caste-related genes in adult diploid males of Melipona quadrifasciata (Hymenoptera, Apidae): a comparison with haploid males, queens and workers
Provedor de dados: Genetics and Molecular Biology
Autores: Borges,Andreia A.; Humann,Fernanda C.; Campos,Lucio A. Oliveira; Tavares,Mara G.; Hartfelder,Klaus.
In Hymenoptera, homozygosity at the sex locus results in the production of diploid males. In social species, these pose a double burden by having low fitness and drawing resources normally spent for increasing the work force of a colony. Yet, diploid males are of academic interest as they can elucidate effects of ploidy (normal males are haploid, whereas the female castes, the queens and workers, are diploid) on morphology and life history. Herein we investigated expression levels of ten caste-related genes in the stingless bee Melipona quadrifasciata, comparing newly emerged and 5-day-old diploid males with haploid males, queens and workers. In diploid males, transcript levels for dunce and paramyosin were increased during the first five days of adult...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stingless bee; Real time PCR; Caste; Diploid male; Differential gene expression.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400025

Molecular identification of Mazama species (Cervidae: Artiodactyla) from natural history collections
Provedor de dados: Genetics and Molecular Biology
Autores: Mantellatto,Aline Meira Bonfim; González,Susana; Duarte,José Maurício Barbanti.
Abstract Natural history museum collections constitute an invaluable patrimony of biological diversity for analysing the taxa distribution and evolution. However, it is very common to discover taxonomic misidentification in museum collections based on incorrect data. The aim of this research was to identify brocket deer species (Mazama genus) using molecular markers. We collected 199 samples, performed DNA extraction and species identification using a specific mitochondrial marker based on a fragment of cytochrome b (Cytb) for Neotropical deer. We achieved the amplification and sequencing of 77 specimens and verified that 26% of the skulls were wrongly identified. Moreover, in the museum collections 57% of the specimens were only identified as Mazama sp,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytochrome b; Deer; DNA; Mammal; Morphological taxonomy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400201

Investigation of the three-dimensional lattice HP protein folding model using a genetic algorithm
Provedor de dados: Genetics and Molecular Biology
Autores: Custódio,Fábio L.; Barbosa,Hélio J. C.; Dardenne,Laurent E.
An approach to the hydrophobic-polar (HP) protein folding model was developed using a genetic algorithm (GA) to find the optimal structures on a 3D cubic lattice. A modification was introduced to the scoring system of the original model to improve the model's capacity to generate more natural-like structures. The modification was based on the assumption that it may be preferable for a hydrophobic monomer to have a polar neighbor than to be in direct contact with the polar solvent. The compactness and the segregation criteria were used to compare structures created by the original HP model and by the modified one. An islands' algorithm, a new selection scheme and multiple-points crossover were used to improve the performance of the algorithm. Ten sequences,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HP model; Genetic algorithms; Protein folding.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400023

Genetic structure of sigmodontine rodents (Cricetidae) along an altitudinal gradient of the Atlantic Rain Forest in southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Gislene L.; Marinho,Jorge R.; Freitas,Thales R.O.
The population genetic structure of two sympatric species of sigmodontine rodents (Oligoryzomys nigripes and Euryoryzomys russatus) was examined for mitochondrial DNA (mtDNA) sequence haplotypes of the control region. Samples were taken from three localities in the Atlantic Rain Forest in southern Brazil, along an altitudinal gradient with different types of habitat. In both species there was no genetic structure throughout their distribution, although levels of genetic variability and gene flow were high.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euryoryzomys russatus; Gene flow; Mismatch distribution; Oligoryzomys nigripes; Population expansion.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400034

Detection of microsatellite instability but not truncating APC mutations in gastric adenocarcinomas in Brazilian patients
Provedor de dados: Genetics and Molecular Biology
Autores: Bevilacqua,Roberta A.U.; Corvello,Cassandra M.; Duarte,Ana Paula; Simpson,Andrew J.G.
A crucial role for the adenomatous polyposis colonic (APC) gene in colorectal carcinogenesis has been conclusively established, but, the role of APC in gastric tumors remains controversial. APC mutations have been detected at a relatively high frequency in gastric tumors of Japanese patients, yet such mutations have been reported to be extremely rare in British patients and patients from north-central-Italy. We here report the analysis of 40 primary sporadic gastric adenocarcinomas and 35 primary sporadic colon adenocarcinomas (from patients resident in São Paulo, Brazil), for mutations in the APC gene between codons 686 and 1693 using the protein truncation test. Although 19 truncating mutations were detected in 35 colon adenocarcinomas (54.2%) none were...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200001

Preface
Provedor de dados: Genetics and Molecular Biology
Autores: Guerra,Marcelo.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400034

Distribution and biological role of the oligopeptide-binding protein (OppA) in Xanthomonas species
Provedor de dados: Genetics and Molecular Biology
Autores: Oshiro,Elisa E.; Tavares,Milene B.; Suzuki,Celso F.; Pimenta,Daniel C.; Angeli,Claudia B.; Oliveira,Julio C.F. de; Ferro,Maria I.T.; Ferreira,Luis C.S.; Ferreira,Rita C.C.
In this study we investigated the prevalence of the oppA gene, encoding the oligopeptide binding protein (OppA) of the major bacterial oligopeptide uptake system (Opp), in different species of the genus Xanthomonas. The oppA gene was detected in two Xanthomonas axonopodis strains among eight tested Xanthomonas species. The generation of an isogenic oppA-knockout derivative of the Xac 306 strain, showed that the OppA protein neither plays a relevant role in oligopeptide uptake nor contributes to the infectivity and multiplication of the bacterial strain in leaves of sweet orange (Citrus sinensis) and Rangpur lime (Citrus limonia). Taken together these results suggest that the oppA gene has a recent evolutionary history in the genus and does not contribute...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xanthomonas; Xac; OppA; Oligopeptide uptake system; ABC transporter.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200023

Combining ability of summer-squash lines with different degrees of parthenocarpy and PRSV-W resistance
Provedor de dados: Genetics and Molecular Biology
Autores: Nogueira,Douglas Willian; Maluf,Wilson Roberto; Figueira,Antonia dos Reis; Maciel,Gabriel Mascarenhas; Gomes,Luiz Antonio Augusto; Benavente,Cesar Augusto Ticona.
The aim was to assess heterosis in a set of 16 summer-squash hybrids, and evaluate the combining capacity of the respective parental lines, which differed as to the degree of parthenocarpy and resistance to PRSV-W (Papaya Ringspot Virus-Watermelon strain). The hybrids were obtained using a partial diallel cross design (4 x 4). The lines of parental group I were 1 = ABX-037G-77-03-05-01-01-bulk, 2 = ABX-037G-77-03-05-03-10-bulk, 3 = ABX-037G77-03-05-01-04-bulk and 4 = ABX-037G-77-03-05-05-01-bulk, and of group II, 1' = ABX-037G-77-03-05-04-08-bulk, 2' = ABX-037G-77-03-05-02-11-bulk, 3' = Clarice and 4' = Caserta. The 16 hybrids and eight parental lines were evaluated for PRSV-W resistance, parthenocarpic expression and yield in randomized complete-block...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cucurbita pepo; Heterosis; Hybrids; Potyvirus; Papaya ringspot virus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400014

Microsatellite-assisted backcross selection in maize
Provedor de dados: Genetics and Molecular Biology
Autores: Benchimol,Luciana Lasry; Souza Jr.,Cláudio Lopes de; Souza,Anete Pereira de.
A microsatellite marker (SSR) was chosen to simulate a target allele and three criteria (02, 04 and 06 markers per chromosome) were tested to evaluate the most efficient parameters for performing marker-assisted backcross (MAB) selection. We used 53 polymorphic SSRs to genotype 186 BC1 maize (Zea mays L.) plants produced by crossing the inbred maize lines L-08-05 (donor parent) and L-14-4B (recurrent parent). The second backcross (BC2) generation was produced with 180 plants and screened with markers which were not recovered from the first backcross (BC1) generation. A total of 480 plants were evaluated in the third backcross (BC3) generation from which 48 plants were selected for parental genotype recovery. Recurrent genotype recovery averages in three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maize; Marker-assisted backcrossing (MAB); Marker-assisted selection; Microsatellites.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500022

Cytogenetic evidence for genome elimination during microsporogenesis in interspecific hybrid between Brachiaria ruziziensis and B. brizantha (Poaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Mendes-Bonato,Andréa Beatriz; Risso-Pascotto,Claudicéia; Pagliarini,Maria Suely; Valle,Cacilda Borges do.
Microsporogenesis was analyzed in an interspecific hybrid between an artificially tetraploidized sexual accession of Brachiaria ruziziensis (R genome) and a natural apomictic tetraploid accession of B. brizantha (B genome). Chromosomes associated predominantly as bivalents. From this phase to the end of meiosis, chromosomes presented irregular segregation and abnormal arrangement in the metaphase plate. During metaphase I, in 27.8% of meiocytes, bivalents were distributed in two metaphase plates. In anaphase I, two distinct and typical bipolar spindles were formed. In 29.7% of pollen mother cells, one genome did not divide synchronically, with chromosomes lagging behind or not segregating at all. The second division was very irregular, resulting in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachiaria; Forage grass; Interspecific hybridization; Meiotic rhythm; Microsporogenesis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400021

Oviduct-specific expression of tissue plasminogen activator in laying hens
Provedor de dados: Genetics and Molecular Biology
Autores: Kaleri,Hubdar Ali; Xiang,Liu; Aniwashi,Jueken; Xu,Shiyong.
Egg-laying hens are important candidate bioreactors for pharmaceutical protein production because of the amenability of their eggs for protein expression. In this study, we constructed an oviduct-specific vector containing tissue plasminogen activator (tPA) protein and green fluorescent protein (pL-2.8OVtPAGFP) and assessed its expression in vitro and in vivo. Oviduct epithelial and 3T3 cells were cultured and transfected with pL-2.8OVtPAGFP and pEGP-N1 (control vector), respectively. The pL-2.8OVtPAGFP vector was administered to laying hens via a wing vein and their eggs and tissues were examined for tPA expression. The oviduct-specific vector pL-2.8OVtPAGFP was expressed only in oviduct epithelial cells whereas pEGP-N1 was detected in oviduct epithelial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Green fluorescent protein; Human tissue plasminogen activator; Laying hens; Oviduct-specific expression.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200011

Molecular evidence for a recent demographic expansion in the puma (Puma concolor) (Mammalia, Felidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Matte,Eunice M.; Castilho,Camila S.; Miotto,Renata A.; Sana,Denis A.; Johnson,Warren E.; O'Brien,Stephen J.; Freitas,Thales R. O. de; Eizirik,Eduardo.
The puma is an iconic predator that ranges throughout the Americas, occupying diverse habitats. Previous phylogeographic analyses have revealed that it exhibits moderate levels of genetic structure across its range, with few of the classically recognized subspecies being supported as distinct demographic units. Moreover, most of the species' molecular diversity was found to be in South America. To further investigate the phylogeographic structure and demographic history of pumas we analyzed mtDNA sequences from 186 individuals sampled throughout their range, with emphasis on South America. Our objectives were to refine the phylogeographic assessment within South America and to investigate the demographic history of pumas using a coalescent approach. Our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Mammals; Mitochondrial DNA; Molecular time estimate; Phylogeography.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400018

Extinction of canid populations by inbreeding depression under stochastic environments in Southwestern Goiás State: a simulation study
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Flávia Melo; Diniz-Filho,José Alexandre Felizola.
A frequently addressed question in conservation biology is what is the chance of survival for a population for a given number of years under certain conditions of habitat loss and human activities. This can be estimated through an integrated analysis of genetic, demographic and landscape processes, which allows the prediction of more realistic and precise models of population persistence. In this study, we modeled extinction in stochastic environments under inbreeding depression for two canid species, the maned wolf (Chrysocyon brachiurus) and the crab-eating fox (Cerdocyon thous), in southwest Goiás State. Genetic parameters were obtained from six microsattelite loci (Short Tandem Repeats - STR), which allowed estimates of inbreeding levels and of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population persistence; Population viability analysis; Canids; STR markers; Parque Nacional das Emas.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100021

Comparison between simulated annealing algorithms and rapid chain delineation in the construction of genetic maps
Provedor de dados: Genetics and Molecular Biology
Autores: Nascimento,Moysés; Cruz,Cosme Damião; Peternelli,Luiz Alexandre; Campana,Ana Carolina Mota.
The efficiency of simulated annealing algorithms and rapid chain delineation in establishing the best linkage order, when constructing genetic maps, was evaluated. Linkage refers to the phenomenon by which two or more genes, or even more molecular markers, can be present in the same chromosome or linkage group. In order to evaluate the capacity of algorithms, four F2 co-dominant populations, 50, 100, 200 and 1000 in size, were simulated. For each population, a genome with four linkage groups (100 cM) was generated. The linkage groups possessed 51, 21, 11 and 6 marks, respectively, and a corresponding distance of 2, 5, 10 and 20 cM between adjacent marks, thereby causing various degrees of saturation. For very saturated groups, with an adjacent distance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Better order; Genetic mapping; Genomic analyses; Stochastic optimization.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200032

Superoxide dismutase, catalase, glutathione peroxidase and gluthatione S-transferases M1 and T1 gene polymorphisms in three Brazilian population groups
Provedor de dados: Genetics and Molecular Biology
Autores: Hiragi,Cássia de Oliveira; Miranda-Vilela,Ana Luisa; Rocha,Dulce Maria Sucena; ,Silviene Fabiana de; Hatagima,Ana; Klautau-Guimarães,Maria de Nazaré.
Antioxidants such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX1) reduce the oxidation rates in the organism. Gluthatione S-transferases (GSTs) play a vital role in phase 2 of biotransformation of many substances. Variation in the expression of these enzymes suggests individual differences for the degree of antioxidant protection and geographical differences in the distribution of these variants. We described the distribution frequency of CAT (21A/T), SOD2 (Ala9Val), GPX1 (Pro198Leu), GSTM1 and GSTT1 polymorphisms in three Brazilian population groups: Kayabi Amerindians (n = 60), Kalunga Afro-descendants (n = 72), and an urban mixed population from Federal District (n = 162). Frequencies of the variants observed in Kalunga...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; PCR-RFLP; Gene polymorphisms; Brazilian ethnicities; Population genetics; Pharmacogenetics.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100003

Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Carolina Gonçalves; Hartfelder,Klaus.
Phenotypic plasticity is a hallmark of the caste systems of social insects, expressed in their life history and morphological traits. These are best studied in bees. In their co-evolution with angiosperm plants, the females of corbiculate bees have acquired a specialized structure on their hind legs for collecting pollen. In the highly eusocial bees (Apini and Meliponini), this structure is however only present in workers and absent in queens. By means of histological sections and cell proliferation analysis we followed the developmental dynamics of the hind legs of queens and workers in the fourth and fifth larval instars. In parallel, we generated subtractive cDNA libraries for hind leg discs of queen and worker larvae by means of a Representational...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Social insect; Caste development; Imaginal disc; Insect metamorphosis; Transcript analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300263

Construction and expression of aspartic protease from Onchocerca volvulus* as ompA fusion protein in a mutant strain of Salmonella typhimurium
Provedor de dados: Genetics and Molecular Biology
Autores: Jolodar,Abbas; Miller,David J.
Two constructions in pHS164 vector were designed to permit expression of OV7A and OV4A inserts encoding the N-terminal and C-terminal portion of an aspartic protease from Onchocerca volvulus, respectively. A novel 39 kD protein ompA-OV7A fusion protein was stably expressed as ompA fusion in a modified strain of Salmonella typhimurium strain SL5000 and E.coli strain JM109. Expression of the fusion protein in bacterial strains harboring the constructs were evaluated by western blotting. E.coli and Salmonella lysates were fractionated by 10% SDS-PAGE gel and then immobilized to nitrocellulose membrane by electroblotting. Primary polyclonal antibody generated in rats against the GST-OV7A fusion protein was used in the Western blots. It remains to be seen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Filarial parasite; Nematode; Salmonella typhimurium.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100018

High levels of chromosomal differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Moura,Rita de Cássia de; Melo,Natoniel Franklin de; Souza,Maria José de.
Euchroma gigantea was karyotypically studied using conventional staining, C-banding, silver nitrate staining and ribosomal fluorescent in situ hybridization (rDNA FISH). Broad wide autosomal polymorphism and a complex sex determination system were found in this beetle. Karyotype complements ranging from 2n = 32, X1X2X3Y1Y2Y3 to 2n = 36,X1X2X3Y1Y2Y3 were detected in the sample analyzed. Punctiform supernumerary chromosomes were present in the different karyotypes. The karyotypic evolution of Brazilian E. gigantea may have taken two directions, reduction in the diploid number of 2n = 36 to 24 through centric fusions or 2n = 24 to 36 due to chromosomal fissions. In addition, pericentric inversions were also involved. The complex multiple sex mechanism of this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome polymorphism; C-banding; Multiple sex system; NOR; RDNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300007

Identification and in silico characterization of soybean trihelix-GT and bHLH transcription factors involved in stress responses
Provedor de dados: Genetics and Molecular Biology
Autores: Osorio,Marina Borges; Bücker-Neto,Lauro; Castilhos,Graciela; Turchetto-Zolet,Andreia Carina; Wiebke-Strohm,Beatriz; Bodanese-Zanettini,Maria Helena; Margis-Pinheiro,Márcia.
Environmental stresses caused by either abiotic or biotic factors greatly affect agriculture. As for soybean [Glycine max (L.) Merril], one of the most important crop species in the world, the situation is not different. In order to deal with these stresses, plants have evolved a variety of sophisticated molecular mechanisms, to which the transcriptional regulation of target-genes by transcription factors is crucial. Even though the involvement of several transcription factor families has been widely reported in stress response, there still is a lot to be uncovered, especially in soybean. Therefore, the objective of this study was to investigate the role of bHLH and trihelix-GT transcription factors in soybean responses to environmental stresses. Gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drought; Gene expression; Glycine max; Phylogeny; Plant-microbe interactions.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200005

Detection and characterization of Wolbachia infection in silkworm
Provedor de dados: Genetics and Molecular Biology
Autores: Zha,Xingfu; Zhang,Wenji; Zhou,Chunyan; Zhang,Liying; Xiang,Zhonghuai; Xia,Qingyou.
Wolbachia naturally infects a wide variety of arthropods, where it plays important roles in host reproduction. It was previously reported that Wolbachia did not infect silkworm. By means of PCR and sequencing we found in this study that Wolbachia is indeed present in silkworm. Phylogenetic analysis indicates that Wolbachia infection in silkworm may have occurred via transfer from parasitic wasps. Furthermore, Southern blotting results suggest a lateral transfer of the wsp gene into the genomes of some wild silkworms. By antibiotic treatments, we found that tetracycline and ciprofloxacin can eliminate Wolbachia in the silkworm and Wolbachia is important to ovary development of silkworm. These results provide clues towards a more comprehensive understanding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wolbachia; Silkworm; Wsp; Antibiotics.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400014

Resistance mutations of NS3 and NS5b in treatment-naïve patients infected with hepatitis C virus in Santa Catarina and Rio Grande do Sul states, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Andrade,Elisabete; Rocha,Daniele; Fontana-Maurell,Marcela; Costa,Elaine; Ribeiro,Marisa; Godoy,Daniela Tupy de; Ferreira,Antonio G.P.; Tanuri,Amilcar; Brindeiro,Rodrigo; Alvarez,Patrícia.
Abstract Hepatitis C virus (HCV) infection is a worldwide health problem. Nowadays, direct-acting antiviral agents (DAAs) are the main treatment for HCV; however, the high level of virus variability leads to the development of resistance-associated variants (RAVs). Thus, assessing RAVs in infected patients is important for monitoring treatment efficacy. The aim of our study was to investigate the presence of naturally occurring resistance mutations in HCV NS3 and NS5 regions in treatment-naïve patients. Ninety-six anti-HCV positive serum samples from blood donors at the Center of Hematology and Hemotherapy of Santa Catarina State (HEMOSC) were collected retrospectively in 2013 and evaluated in this study. HCV 1a (37.9%), 1b (25.3%), and 3a (36.8%) subtypes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Direct-acting antivirals; Resistance-associated substitutions; Blood donors; NS3; NS5b.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100107

Cytotoxic and genotoxic effects of Br-containing oxaphosphole on Allium cepa L. root tip cells and mouse bone marrow cells
Provedor de dados: Genetics and Molecular Biology
Autores: Kalcheva,Vanya P.; Dragoeva,Asya P.; Kalchev,Karamfil N.; Enchev,Dobromir D.
The continuous production and release of chemicals into the environment has led to the need to assess their genotoxicity. Numerous organophosphorus compounds with different structures have been synthesized in recent years, and several oxaphosphole derivatives are known to possess biological activity. Such chemical compounds may influence proliferating cells and cause disturbances of the genetic material. In this study, we examined the cytotoxicity and genotoxicity of 4-bromo-N,N-diethyl-5,5-dimethyl-2,5-dihydro-1,2-oxaphosphol-2-amine 2-oxide (Br-oxph). In A. cepa cells, Br-oxph (10-9 M, 10-6 M and 10-3 M) reduced the mitotic index 48 h after treatment with the two highest concentrations, with no significant effect at earlier intervals. Mitotic cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa L. root tip cells; Br-containing oxaphosphole derivative; Chromosome aberrations; ICR mouse bone marrow.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200028

Chromosomes of Theridiidae spiders (Entelegynae): interspecific karyotype diversity in Argyrodes and diploid number intraspecific variability in Nesticodes rufipes
Provedor de dados: Genetics and Molecular Biology
Autores: Stavale,Leila Miguel; Schneider,Marielle Cristina; Araujo,Douglas; Brescovit,Antonio Domingos; Cella,Doralice Maria.
Theridiidae is a derived family within the Araneoidea clade. In contrast to closely related groups, the 2n(male) = 20+X1X2 with acro/telocentric chromosomes is the most widespread karyotype among the theridiid spiders. In this work, the cytogenetic analysis of Argyrodes elevatus revealed original chromosome features different from those previously registered for Theridiidae, including the presence of 2n(male) = 20+X with meta/submetacentric chromosomes. Most individuals of Nesticodes rufipes showed family conserved karyotype characteristics. However, one individual had a 2n(male) = 24 due to the presence of an extra chromosome pair, which exhibited regular behavior and reductional segregation during meiosis. After silver staining, mitotic cells exhibited...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome morphology; Cytogenetic; Meiosis; Nucleolar organizer region; Sex chromosome system.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400012

Prevalence of common α-thalassemia determinants in south Brazil: importance for the diagnosis of microcytic anemia
Provedor de dados: Genetics and Molecular Biology
Autores: Wagner,Sandrine C.; Castro,Simone M. de; Gonzalez,Tatiana P.; Santin,Ana P.; Filippon,Leticia; Zaleski,Carina F.; Azevedo,Laura A.; Amorin,Bruna; Callegari-Jacques,Sidia M.; Hutz,Mara H.
Alpha thalassemia has not been systematically investigated in Brazil. In this study, 493 unrelated individuals from the southernmost Brazilian state of Rio Grande do Sul were screened for deletional forms of α-thalassemia. One hundred and one individuals had microcytic anemia (MCV &lt; 80 fL) and a normal hemoglobin pattern (Hb A2 &lt; 3.5% and Hb F &lt; 1%). The subjects were screened for -α3.7,-α4.2,-α20.5, -SEA and -MED deletions but only the -α3.7 allele was detected. The -α3.7 allele frequency in Brazilians of European and African ancestry was 0.02 and 0.12, respectively, whereas in individuals with microcytosis the frequency was 0.20. The prevalence of α-thalassemia was significantly higher in individuals with microcytosis than in healthy individuals...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-thalassemia; Brazilian population; Genotype; Hemoglobin; Microcytosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400008

Sewage sludge does not induce genotoxicity and carcinogenesis
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Paula Regina Pereira; Barbisan,Luis Fernando; Dagli,Maria Lúcia Zaidan; Saldiva,Paulo Hilário Nascimento.
Through a series of experiments, the genotoxic/mutagenic and carcinogenic potential of sewage sludge was assessed. Male Wistar rats were randomly assigned to four groups: Group 1 - negative control; Group 2 - liver carcinogenesis initiated by diethylnitrosamine (DEN; 200 mg/kg i.p.); Group 3 and G4- liver carcinogenesis initiated by DEN and fed 10,000 ppm or 50,000 ppm of sewage sludge. The animals were submitted to a 70% partial hepatectomy at the 3rd week. Livers were processed for routine histological analysis and immunohistochemistry, in order to detect glutathione S-transferase positive altered hepatocyte foci (GST-P+ AHF). Peripheral blood samples for the comet assay were obtained from the periorbital plexus immediately prior to sacrificing....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Micronucleus; Sewage genotoxicity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400017

Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips
Provedor de dados: Genetics and Molecular Biology
Autores: Matsumoto,Silvia Tamie; Mantovani,Mário Sérgio; Malaguttii,Mirtis Irene Ariza; Dias,Ana Lúcia; Fonseca,Inês Cristina; Marin-Morales,Maria Aparecida.
Cytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs) which generate chromosomal aberrations, micronucleus formation, sister chromatid exchange, formation of DNA adducts and alterations in DNA replication and transcription. In our study, water samples from three sites in the Córrego dos Bagres stream in the Franca municipality of the Brazilian state of São Paulo were subjected to the comet assay and micronucleus test using erythrocytes from the fish Oreochromis niloticus. Nuclear abnormalities of the erythrocytes included blebbed, notched and lobed nuclei, probably due to genotoxic chromium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Chromium; Chromosomes aberrations; Comet assay; Micronucleus; Oreochromis niloticus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100028

Maternal transmission of mitochondrial diseases
Provedor de dados: Genetics and Molecular Biology
Autores: Chiaratti,Marcos R.; Macabelli,Carolina H.; Augusto Neto,José Djaci; Grejo,Mateus Priolo; Pandey,Anand Kumar; Perecin,Felipe; Collado,Maite del.
Abstract Given the major role of the mitochondrion in cellular homeostasis, dysfunctions of this organelle may lead to several common diseases in humans. Among these, maternal diseases linked to mitochondrial DNA (mtDNA) mutations are of special interest due to the unclear pattern of mitochondrial inheritance. Multiple copies of mtDNA are present in a cell, each encoding for 37 genes essential for mitochondrial function. In cases of mtDNA mutations, mitochondrial malfunctioning relies on mutation load, as mutant and wild-type molecules may co-exist within the cell. Since the mutation load associated with disease manifestation varies for different mutations and tissues, it is hard to predict the progeny phenotype based on mutation load in the progenitor. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oocyte; Germline; Mitochondrial dynamics; MtDNA; Metabolism.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200308

Structure and genetic variability in Nellore (Bos indicus) cattle by pedigree analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Vozzi,Pedro Alejandro; Marcondes,Cíntia Righetti; Magnabosco,Cláudio de Ulhôa; Bezerra,Luiz Antonio Framartino; Lôbo,Raysildo Barbosa.
Parameters based on the probability of gene origin were used to describe the genetic variability in strains of Nellore and polled Nellore (Bos indicus) cattle that participated in the Program for Genetic Improvement of the Nellore Breed (Programa de Melhoramento Genético da Raça Nelore). The effective number of founders was 87.2 for Nellore and 107.9 for polled Nellore, while the number of ancestors was 59.8 for Nellore and 61.5 for polled Nellore and the remaining genomes were 39.4 for Nellore and 34.5 for polled Nellore cattle. The results indicate an intense use (by artificial insemination) of some sires and the absence of subdivisions in the population. The family structure in the two breeds was mainly caused by the genetic contribution of the same...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic drift; Genetic variability matings; Nellore; Pedigree analysis; Probability of gene origin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300014

Identification of protein kinase SNF1 in CitEST
Provedor de dados: Genetics and Molecular Biology
Autores: Guidetti-Gonzalez,Simone; Ragassi,Carlos Francisco; Carrer,Helaine.
SnRKs (Sucrose non-fermenting-1 related kinases) is a family of protein kinases found in many crops, such as Arabidopsis, rice, sugarcane, tomato and several other plant species. This family of proteins is also present in other organisms like Saccharomyces cerevisiae (sucrose non-fermenting-1 - Snf1) and in mammals (AMP-activated protein kinases - AMPKs). There is evidence that SnRKs play an important role in plant responses to nutritional and environmental stresses and that SnRKs also play a major role in controlling key enzymes in the biosynthetic pathways of plants. In this work, we identified 18 contigs and two singletons encoding putative SnRKs in the CitEST database. All of them present highly conserved N-terminal catalytic domain, which is found in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SnRK; Sucrose non-fermenting-1 (SNF1); Transcription regulator.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500015

Agronomic, cytogenetic, and isoenzymatic characterizations of oat somaclones
Provedor de dados: Genetics and Molecular Biology
Autores: Augustin,Lizete; Federizzi,Luiz Carlos; Sereno,Maria Jane Cruz de Melo.
Immature embryo-derived somaclones regenerated from genotypes UPF 12, UPF 89S080 and UFRGS 7 were analyzed for eight agronomic traits and two enzymatic systems in order to evaluate the potential of tissue cultures to induce genetic variability in oats (Avena sativa L.). Some somaclones were also analyzed cytogenetically. Agronomic traits were evaluated in the field for all somaclones in 1993 and 1994. Bi-directional variation (P &lt; 0.05) was detected for all characteristics, and the average frequency of variation observed in 1993 in somaclones from genotypes UPF 12 and UPF 89S080 was 35.2%. Variation observed for agronomic characteristics was heritable through two generations. Isoenzymatic analysis showed variation for both enzymatic systems in four...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300025

A prevalent alpha-proteobacterium Paracoccus sp. in a population of the Cayenne ticks (Amblyomma cajennense) from Rio de Janeiro, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Machado-Ferreira,Erik; Piesman,Joseph; Zeidner,Nordin S; Soares,Carlos A.G.
As Rocky Mountain Spotted Fever is the most common tick-borne disease in South America, the presence of Rickettsia sp. in Amblyomma ticks is a possible indication of its endemicity in certain geographic regions. In the present work, bacterial DNA sequences related to Rickettsia amblyommii genes in A. dubitatum ticks, collected in the Brazilian state of Mato Grosso, were discovered. Simultaneously, Paracoccus sp. was detected in aproximately 77% of A. cajennense specimens collected in Rio de Janeiro, Brazil. This is the first report of Paracoccus sp. infection in a specific tick population, and raises the possibility of these bacteria being maintained and/or transmitted by ticks. Whether Paracoccus sp. represents another group of pathogenic Rhodobacteraceae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paracoccus; Rickettsia amblyommii; Amblyomma; Ixodidae ticks.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500021

Genetic structure of honeybee populations from southern Brazil and Uruguay
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz,Nilza Maria; Soares,Ademilson Espencer Egea; Sheppard,Walter Steve; Del Lama,Marco Antonio.
Apis mellifera scutellata was introduced to Brazil in 1956 and Africanized honeybee populations have now spread from Argentina to the southwestern United States. Temperate climatic restrictions seem to be a natural limit to Africanized honeybee expansion around parallels 35° to 40° SL. We used allozyme loci (Mdh-1 and Hk-1) and mtDNA haplotypes to characterize honeybee populations in southern Brazil and Uruguay and define a possible transition area between Africanized and European bees. Samples of 194 bee colonies were collected from ten localities between 30°-35° SL and 52°-59° WL. The mtDNA restriction patterns of these colonies were obtained through digestion of the mitochondrial genome by Eco RI, or by digestion by Bgl II and Xba I of the cytochrome B...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Africanized honeybees; Transition area; South America; MtDNA; Allozymes; Population genetics.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100008

Large-scale analysis of NBS domain-encoding resistance gene analogs in Triticeae
Provedor de dados: Genetics and Molecular Biology
Autores: Bouktila,Dhia; Khalfallah,Yosra; Habachi-Houimli,Yosra; Mezghani-Khemakhem,Maha; Makni,Mohamed; Makni,Hanem.
Proteins containing nucleotide binding sites (NBS) encoded by plant resistance genes play an important role in the response of plants to a wide array of pathogens. In this paper, an in silico search was conducted in order to identify and characterize members of NBS-encoding gene family in the tribe of Triticeae. A final dataset of 199 sequences was obtained by four search methods. Motif analysis confirmed the general structural organization of the NBS domain in cereals, characterized by the presence of the six commonly conserved motifs: P-loop, RNBS-A, Kinase-2, Kinase-3a, RNBS-C and GLPL. We revealed the existence of 11 distinct distribution patterns of these motifs along the NBS domain. Four additional conserved motifs were shown to be significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NBS domain; Data mining; Phylogeny; Plant resistance genes; Triticeae.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400017

Mutagenic effects of tributyltin and inorganic lead (Pb II) on the fish H. malabaricus as evaluated using the comet assay and the piscine micronucleus and chromosome aberration tests
Provedor de dados: Genetics and Molecular Biology
Autores: Ferraro,Marcos Vinícius M.; Fenocchio,Alberto Sérgio; Mantovani,Mario Sérgio; Ribeiro,Ciro de Oliveira; Cestari,Marta Margarete.
Genotoxicity studies on toxic metals and their organic compounds are very important, especially so in the investigation of the effects of these compounds on the aquatic environments where they tend to accumulate. The use of endemic aquatic organisms as biological sentinels has proved useful to environmental monitoring. We assessed the mutagenic potential of tributyltin (TBT) and inorganic lead (PbII) using samples of the fish Hoplias malabaricus (commonly called traíra) using the comet assay and the piscine micronucleus and chromosome aberration tests. Eighteen H. malabaricus were acclimatized in three individual aquariums, each containing six fish, six fish being exposed to 0.3 mg/g of body weight (bw) of TBT, six to 21 mg/g bw of PbII and six being used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Micronucleus; Chromosome aberrations; Tributyltin; Lead; Fish.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100017

Cytogenetic study of the giant otter Pteronura brasiliensis Zimmermann 1780 (Carnivora, Mustelidae, Lutrinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Franco-de-Sá,Jorge Felipe Oliveira; Rosas,Fernando César Weber; Feldberg,Eliana.
The giant otter, Pteronura brasiliensis Zimmermann 1780 (Carnivora, Mustelidae, Lutrinae), was widely distributed in South America but stable populations are now only found in the Pantanal and Amazon regions and the species is classified as endangered. There is only one recognized species of giant otter, although two subspecies of doubtful value have also been cited in the literature. We present the first karyotype of four captive P. brasiliensis specimens, all of which posses 2n = 38 chromosomes as 14M+8SM+6ST+8A and one pair of sexual chromosomes. An heteromorphic secondary constriction, associated with the nucleolar organizer region (NOR), was seen on the long arms of chromosome pair 17. The C-banding technique revealed heterochromatin in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon; Aquatic mammals; Ariranha; Karyotype.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600011

Immunofluorescence in cytogenetic analysis: method and applications
Provedor de dados: Genetics and Molecular Biology
Autores: Jeppesen,Peter.
Control of the genetic information encoded by DNA in mammalian chromosomes is mediated by proteins, some of which are only transiently attached, although others are intrinsically associated with nucleic acid in the complex mixture known as chromatin. Chromatin-associated proteins range from the ubiquitous and abundant histones down to the most specific and rare of transcription factors. Although many chromatin proteins are probably excluded from highly condensed mitotic chromosomes, a number are retained throughout the cell cycle and can be detected on chromosomes in metaphase spreads. Comparing the distribution of a chromosomal protein with known cytogenetic markers on metaphase chromosomes can provide an important and potentially highly informative first...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400059

Alternative parameterizations of relatedness in whole genome association analysis of pre-weaning traits of Nelore-Angus calves
Provedor de dados: Genetics and Molecular Biology
Autores: Riley,David G.; Gill,Clare A.; Herring,Andy D.; Riggs,Penny K.; Sawyer,Jason E.; Sanders,James O.
Gestation length, birth weight, and weaning weight of F2 Nelore-Angus calves (n = 737) with designed extensive full-sibling and half-sibling relatedness were evaluated for association with 34,957 SNP markers. In analyses of birth weight, random relatedness was modeled three ways: 1) none, 2) random animal, pedigree-based relationship matrix, or 3) random animal, genomic relationship matrix. Detected birth weight-SNP associations were 1,200, 735, and 31 for those parameterizations respectively; each additional model refinement removed associations that apparently were a result of the built-in stratification by relatedness. Subsequent analyses of gestation length and weaning weight modeled genomic relatedness; there were 40 and 26 trait-marker associations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birth weight; Gestation length; Weaning weight; Nelore; Whole genome association.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400007

Genomic rearrangements in BRCA1 and BRCA2: a literature review
Provedor de dados: Genetics and Molecular Biology
Autores: Ewald,Ingrid Petroni; Ribeiro,Patricia Lisboa Izetti; Palmero,Edenir Inêz; Cossio,Silvia Liliana; Giugliani,Roberto; Ashton-Prolla,Patricia.
Women with mutations in the breast cancer genes BRCA1 or BRCA2 have an increased lifetime risk of developing breast, ovarian and other BRCA-associated cancers. However, the number of detected germline mutations in families with hereditary breast and ovarian cancer (HBOC) syndrome is lower than expected based upon genetic linkage data. Undetected deleterious mutations in the BRCA genes in some high-risk families are due to the presence of intragenic rearrangements such as deletions, duplications or insertions that span whole exons. This article reviews the molecular aspects of BRCA1 and BRCA2 rearrangements and their frequency among different populations. An overview of the techniques used to screen for large rearrangements in BRCA1 and BRCA2 is also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BRCA1; BRCA2; Breast cancer; Genomic rearrangements; MLPA.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300003

Frequency and spectrum of hemoglobinopathy mutations in a Uruguayan pediatric population
Provedor de dados: Genetics and Molecular Biology
Autores: Luz,Julio Da; Ávila,Amalia; Icasuriaga,Sandra; Gongóra,María; Castillo,Luis; Serrón,Alejandra; Kimura,Elza Miyuki; Costa,Fernando Ferreira; Sans,Mónica; Sonati,Maria de Fátima.
Hemoglobinopathies are the most common recessive diseases worldwide but their prevalence in Uruguay has not been investigated. In this study, 397 unrelated outpatient children from the Pereira Rosell Hospital Center (CHPR), as well as 31 selected patients with microcytic anemia and 28 β-thalassemia carriers were analyzed for hemoglobinopathies by using biochemical and molecular biology methods. Parametric and non-parametric methods were used to compare the hematological indices between groups of genotypes. Of the 397 patients in the first group, approximately 1% (0.76% HbS and 0.25% β-thalassemia) had a mutation in the HBB gene and 3.3% had α-thalassemia. These mutations had a heterogeneous distribution that varied according to individual ancestry. HbS was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-globin; Beta-globin; Hemoglobinopathies; Uruguayan population.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300003

Genetic analysis of reciprocal differences in the inheritance of in vitro characters in pearl millet
Provedor de dados: Genetics and Molecular Biology
Autores: Satyavathi,Valluri V; Manga,V.; Rao,Muktinutalapati V. Subba; Chittibabu,Malladi.
Abstract Reciprocal differences persist in nature because of the unequal contribution of cytoplasmic determinants from male and female gametes to the zygote. The inheritance of genetic differences is an important factor that influences various traits, including somatic embryogenesis and regeneration in vitro. In this report, we estimate the cytoplasmic and maternal effects in pearl millet and their adequacy in describing the observed reciprocal differences based on an in depth study of the parents, F2s and reciprocal backcross progenies needed for fitting genetical models. Our study revealed that of the two characters examined, embryogenic callus quantity and regeneration frequency, the former showed a greater proportion of cytoplasmic nuclear interaction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytoplasmic inheritance; D2 dwarf; In vitro regeneration; Pearl millet; Reciprocal differences.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100054

Ecophysiology, genotoxicity, histopathology, and gene responses of naphthalene injected Colossoma macropomum (Cuvier, 1818) exposed to hypoxia
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Samara Silva de; Silva,Grazyelle Sebrenski da; Almeida-Val,Vera Maria Fonseca de.
Abstract The present study aimed to evaluate the biological responses of Colossoma macropomum to naphthalene injection and subsequent hypoxia exposure, emphasizing the expression of the tumor suppressor gene tp53. Tambaquis were intraperitoneally injected with naphthalene (50 mg/kg) and, after 96 hours, the fish were transferred to respirometry chambers and, submitted to progressive hypoxia for the determination of critical PO2. In a subsequent experiment, the fish received an intraperitoneal injection of naphthalene and were kept for 96 hours under normoxia. Successively, fish were challenged with acute hypoxia (PO2&lt;PO2 crit) during 6 hours. We observed that the PO2 crit was not affected by naphthalene injection. Moreover, hematological parameters were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Naphthalene; Hypoxia; Tumor suppressor gene tp53; DNA damage; Tambaqui.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300411

In silico identification of known osmotic stress responsive genes from Arabidopsis in soybean and Medicago
Provedor de dados: Genetics and Molecular Biology
Autores: Soares-Cavalcanti,Nina M.; Belarmino,Luis C.; Kido,Ederson A.; Wanderley-Nogueira,Ana C.; Bezerra-Neto,João P.; Cavalcanti-Lira,Rafaela; Pandolfi,Valesca; Nepomuceno,Alexandre L.; Abdelnoor,Ricardo V.; Nascimento,Leandro C.; Benko-Iseppon,Ana M.
Plants experience various environmental stresses, but tolerance to these adverse conditions is a very complex phenomenon. The present research aimed to evaluate a set of genes involved in osmotic response, comparing soybean and medicago with the well-described Arabidopsis thaliana model plant. Based on 103 Arabidopsis proteins from 27 categories of osmotic stress response, comparative analyses against Genosoja and Medicago truncatula databases allowed the identification of 1,088 soybean and 1,210 Medicago sequences. The analysis showed a high number of sequences and high diversity, comprising genes from all categories in both organisms. Genes with unknown function were among the most representative, followed by transcription factors, ion transport...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osmotic stress; Stress-responsive genes; Glycine max; Medicago truncatula.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200012

Chromosome evolution in tiger beetles: Karyotypes and localization of 18S rDNA loci in Neotropical Megacephalini (Coleoptera, Cicindelidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Proença,Sónia J.R.; Collares-Pereira,Maria João; Serrano,Artur R.M.
Four Neotropical tiger beetle species, three from the genus Megacephala and one from the genus Oxycheila, currently assigned to the tribe Megacephalini were examined cytogenetically. All three Megacephala species showed simple sex chromosome systems of the X0/XX type but different numbers of autosomal pairs (15 in M. cruciata, 14 in M. sobrina and 12 in M. rutilans), while Oxycheila tristis was inferred to have a multiple sex chromosome system with four X chromosomes (2n = 24 + X1X2X3X4Y/X1X1X2X2X3X3X4X4). Fluorescence in situ hybridization (FISH) using a PCR-amplified 18S rDNA fragment as a probe revealed the presence of rDNA clusters located exclusively on the autosomes in all the Megacephala species (five clusters in M. cruciata, eight in M. sobrina and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome evolution; Cicindelidae; Megacephalini; Oxycheila; RDNA localization; Sex chromosome system.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500012

Development of a Brazilian maize core collection
Provedor de dados: Genetics and Molecular Biology
Autores: Coimbra,Ronaldo R.; Miranda,Glauco V.; Cruz,Cosme D.; Silva,Derly J.H.; Vilela,Ramiro A.
The aim of this study was to evaluate methods for developing a Brazilian maize core collection. For an initial survey of the active collection, passport information, as well as characterization and evaluation of accessions, were taken into consideration, these then being divided according to geographic region and kernel-type. Multiple sampling methods were evaluated. The strategy of constant sampling generated extensive alterations in extract accession frequency. The multivariate strategy with dispersion graphs and principal components associated with the Tocher method was considered efficient for identifying the most divergent genotypes. The multivariate strategy generated greater alterations in the variance of traits. The average number of traits...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germplasm collection; Zea mays; Breeding; Landraces; Selection.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300017

Molecular characterization and T and B cell epitopes prediction of Mycoplasma synoviae 53 strain VlhA hemagglutinin
Provedor de dados: Genetics and Molecular Biology
Autores: Camargo,Ilana Lopes; Fonseca,Cristina Toscano; Teixeira,Santuza Ribeiro; Azevedo,Vasco; Myioshi,Anderson; Oliveira,Sergio Costa.
Mycoplasma sinoviae is a major pathogen of poultry causing synovitis and respiratory infection. M. synoviae hemagglutinin (VlhA) is a lipoprotein encoded by related multigene families that appear to have arisen by horizontal gene transfer. It is an abundant immunodominant surface protein involved in host-parasite interaction mediating binding to host erythrocytes. Herein, we have performed in silico analysis of the vlhA gene product from the Mycoplasma synoviae 53 strain and compared it to the VlhA protein of M. synoviae WUV1853 strain. The VlhA of the M. synoviae 53 strain possesses 569 amino acids and showed 85% identity with the VlhA protein of the M. synoviae WUV1853 strain. Further, a signal peptide was identified from amino acid M1 to D28 and a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma synoviae; Hemagglutinin; Epitopes; Host-parasite interaction; Vaccine.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200013

Performance of probe polymerization-conjunction-agarose gel electrophoresis in the rapid detection of KRAS gene mutation
Provedor de dados: Genetics and Molecular Biology
Autores: Xiao,Na; Tang,Yi-Tong; Li,Zhi-Shan; Cao,Rui; Wang,Rong; Zou,Jiu-Ming; Pei,Jiao.
Abstract This study aimed to develop a simple and rapid method to detect KRAS gene mutations for conventional clinical applications under laboratory conditions. The genotype of mutation sites was determined based on the occurrence of target bands in the corresponding lanes of the reaction tubes through polymerization-conjunction of the probes, probe purification and amplification, and agarose gel electrophoresis. Circulating DNA samples were obtained from the plasma of 72 patients with lung cancer, which were identified based on six mutation sites (G12S, G12R, G12C, G12D, G12A, and G12V) of codon 12 of the KRAS gene. The detection results were compared with direct sequencing data. The proposed detection method is characterized by simple operation, high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutation; Circulating DNA; Polymerization-conjunction reaction; Agarose gel electrophoresis; K-ras.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400555

Phylogenetic analysis of the order Pleuronectiformes (Teleostei) based on sequences of 12S and 16S mitochondrial genes
Provedor de dados: Genetics and Molecular Biology
Autores: Azevedo,Marisa F.C.; Oliveira,Claudio; Pardo,Belén G.; Martínez,Paulino; Foresti,Fausto.
The fish order Pleuronectiformes, composed of 14 families, has two suborders: Psettodoidei (with one family) and Pleuronectoidei (with thirteen families). The relationships among families of Pleuronectoidei and among the genera of their families have extensively been debated and a consensus has not yet been reached. In the present study, partial sequences of the 12S and 16S mitochondrial rRNA genes were obtained from 19 species belonging to the families Achiridae, Bothidae, Cynoglossidae, Paralichthyidae, Pleuronectidae, Scophthalmidae, and Soleidae. Additional sequences of 42 pleuronectiform species were obtained from GenBank. Phylogenetic analyses were conducted by the methods of maximum-parsimony, maximum-likelihood and Bayesian inference. Our results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogeny; Molecular systematics; Mitochondrial DNA; Fish evolution; Flatfishes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200023

Looking for exceptions on knowledge rules induced from HIV cleavage data set
Provedor de dados: Genetics and Molecular Biology
Autores: Prati,Ronaldo Cristiano; Monard,Maria Carolina; Carvalho,André C. P. L. F. de.
The aim of data mining is to find useful knowledge inout of databases. In order to extract such knowledge, several methods can be used, among them machine learning (ML) algorithms. In this work we focus on ML algorithms that express the extracted knowledge in a symbolic form, such as rules. This representation may allow us to ''explain'' the data. Rule learning algorithms are mainly designed to induce classification rules that can predict new cases with high accuracy. However, these sorts of rules generally express common sense knowledge, resulting in many interesting and useful rules not being discovered. Furthermore, the domain independent biases, especially those related to the language used to express the induced knowledge, could induce rules that are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Data mining; Exceptions; HIV; Protease.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400027

Association of killer cell immunoglobulin-like receptor polymorphisms with chronic hepatitis C and responses to therapy in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Vasconcelos,Janaina Mota de; Móia,Lizomar de Jesus Maués Pereira; Amaral,Ivanete do Socorro Abraçado; Miranda,Esther Castello Branco Mello; CicaliseTakeshita,Louise Yukari; Oliveira,Layanna Freitas de; Mendes,Lilian de Araújo Melo; Sastre,Danuta; Tamegão-Lopes,Bruna Pedroso; Pedroza,Larysse Santa Rosa de Aquino; Santos,Sidney Emanuel Batista dos; Soares,Manoel do Carmo Pereira; Araújo,Marialva Tereza Ferreira de; Bandeira,Camila Lucas; Silva,Adriana Maria Paixão de Sousa da; Medeiros,Zilene Lameira de; Sena,Leonardo; Demachki,Samia; Santos,Eduardo José Melo dos.
Soroprevalence for Hepatitis C virus is reported as 2.12% in Northern Brazil, with about 50% of the patients exhibiting a sustained virological response (SVR). Aiming to associate polymorphisms in Killer Cell Immunoglobulin-like Receptors (KIR) with chronic hepatitis C and therapy responses we investigated 125 chronic patients and 345 controls. Additionally, 48 ancestry markers were genotyped to control for population stratification. The frequency of the KIR2DL2 and KIR2DL2+HLA-C Asp80 gene and ligand was higher in chronic infected patients than in controls (p &lt; 0.0009, OR = 3.4; p = 0.001, OR = 3.45). In fact, KIR2DL3 is a weaker inhibitor of NK activity than KIR2DL2, which could explain the association of KIR2DL2 with chronic infection. Moreover,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; KIR; HLA-C; Hepatitis C; KIR2DL2.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100004

Novel 12S mtDNA findings in sloths (Pilosa, Folivora) and anteaters (Pilosa, Vermilingua) suggest a true case of long branch attraction
Provedor de dados: Genetics and Molecular Biology
Autores: Barros,Maria Claudene; Sampaio,Iracilda; Schneider,Horacio.
We sequenced 12S RNA mtDNA for the majority of the extant species of sloths and anteaters and compared our results with previous data obtained by our group using 16S RNA mtDNA in the same specimens and to GenBank sequences of the extinct giant sloth Mylodon. Our results suggest that pigmy-anteaters may be a case of the long-branch attraction phenomenon and also show the large genetic difference between the Amazonian and Atlantic forest three-toed sloths, contrasting with the small differences observed between the two non-Atlantic forest forms of sloths. These results have important implications for the taxonomy of sloths and anteaters and strongly suggest the placement of pigmy anteaters in their own family (Cyclopidae) and raising the taxonomic status of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anteaters; Molecular evolution; Mylodont; Sloths; Xenarthra.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400029

Identification and characterization of MAGO and Y14 genes in Hevea brasiliensis
Provedor de dados: Genetics and Molecular Biology
Autores: Yang,Zi-Ping; Li,Hui-Liang; Guo,Dong; Peng,Shi-Qing.
Abstract Mago nashi (MAGO) and Y14 proteins are highly conserved among eukaryotes. In this study, we identified two MAGO (designated as HbMAGO1 andHbMAGO2) and two Y14 (designated as HbY14aand HbY14b) genes in the rubber tree (Hevea brasiliensis) genome annotation. Multiple amino acid sequence alignments predicted that HbMAGO and HbY14 proteins are structurally similar to homologous proteins from other species. Tissue-specific expression profiles showed that HbMAGO and HbY14 genes were expressed in at least one of the tissues (bark, flower, latex, leaf and root) examined. HbMAGOs and HbY14s were predominately located in the nucleus and were found to interact in yeast two-hybrid analysis (YTH) and bimolecular fluorescence complementation (BiFC) assays....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interaction; Hevea brasiliensis; Mago nashi; Y14 proteins.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100073

Genome sequence of the H2-producing Clostridium beijerinckii strain Br21 isolated from a sugarcane vinasse treatment plant
Provedor de dados: Genetics and Molecular Biology
Autores: Fonseca,Bruna Constante; Riaño-Pachón,Diego Mauricio; Guazzaroni,María-Eugenia; Reginatto,Valeria.
Abstract We report on the nearly complete genome sequence of Clostridium beijerinckii strain Br21, formerly isolated from a sugarcarne vinasse wastewater treatment plant. The resulting genome is ca. 5.9 Mbp in length and resembles the size of previously published C. beijerinckii genomes. We annotated the genome sequence and predicted a total of 5323 genes. Strain Br21 has a genetic toolkit that allows it to exploit diverse sugars that are often found after lignocellulosic biomass pretreatment to yield products of commercial interest. Besides the whole set of genes encoding for enzymes underlying hydrogen production, the genome of the new strain includes genes that enable carbon sources conversion into butanol, ethanol, acetic acid, butyric acid, and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium; Biofuels; Biohydrogen; Beta-glucosidase.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100139

Multivariate analysis of combining ability for soybean resistance to Cercospora sojina Hara
Provedor de dados: Genetics and Molecular Biology
Autores: Gravina,Geraldo de Amaral; Sediyama,Carlos Sigueyuki; Martins Filho,Sebastião; Moreira,Maurílio Alves; Barros,Everaldo Gonçalves de; Cruz,Cosme Damião.
Seven soybean cultivars (Bossier, Cristalina, Davis, Kent, Lincoln, Paraná and Uberaba), with different levels of resistance to Cercospora sojina, race 04, were crossed according to a diallel design, with no reciprocals, to determine the general and the specific combining abilities for the resistance. The evaluations of the reaction to the disease were performed 20 days after the inoculation of the fungus on the most infected leaflet of the plant, in the parents and in the F1 hybrids. To quantify the resistance, the following characteristics were evaluated: infection degree (ID); number of lesions per leaflet (NLL); lesion mean diameter (LMD); lesioned leaf area (LLA); percentage of lesioned leaf area (PLLA); number of lesions per square centimeter (NLC)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Diallel; Canonical variables; Frogeye leaf spot.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300015

Functional abilities of cultivable plant growth promoting bacteria associated with wheat (Triticum aestivum L.) crops
Provedor de dados: Genetics and Molecular Biology
Autores: Moreira,Fernanda da S.; Costa,Pedro B. da; Souza,Rocheli de; Beneduzi,Anelise; Lisboa,Bruno B.; Vargas,Luciano K.; Passaglia,Luciane M. P.
Abstract In the pursuit of sustainable agriculture, bioinoculants usage as providers of a crop's needs is a method to limit environmental damage. In this study, a collection of cultivable putative plant growth promoting (PGP) bacteria associated with wheat crops was obtained and this bacterial sample was characterized in relation to the functional diversity of certain PGP features. The isolates were obtained through classical cultivation methods, identified by partial 16S rRNA gene sequencing and characterized for PGP traits of interest. Functional diversity characterization was performed using Categorical Principal Component Analysis (CatPCA) and Multiple Correspondence Analysis (MCA). The most abundant genera found among the 346 isolates were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant growth promoting bacteria; Categorical Principal Component Analysis; Functional analysis; Burkholderia genus.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100111

Molecular analysis of phylogeographic subspecies in three Ponto-Caspian sturgeon species
Provedor de dados: Genetics and Molecular Biology
Autores: Dudu,Andreea; Georgescu,Sergiu Emil; Costache,Marieta.
Sturgeons (Order Acipenseriformes) represent an extremely valuable natural resource that is now facing depletion. In the current study we evaluate if the traditional classification in subspecies of Acipenser gueldenstaedtii, Acipenser stellatus and Huso huso, endemic to Ponto-Caspian region is sustained by molecular analysis and if these represent Evolutionary Significant Units (ESUs) that should be managed separately in conservation programs. To examine the classification of taxonomic entities we sequenced a fragment of the mitochondrial control region in case of three sturgeon species that inhabit the North-western of Black Sea and migrate for reproduction in the Lower Danube. Beside these sequences, we used previously published sequences from sturgeon...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ponto-Caspian sturgeon; Subspecies; ESUs; Mitochondrial markers.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400016

Assessment of the toxicogenic effects and cell death potential of the ester (Z)-methyl 4-((1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-yl)amino)-4-oxobut-2-anoate in combination with cisplatin, cyclophosphamide and doxorubicin
Provedor de dados: Genetics and Molecular Biology
Autores: Juliano Oliveira,Rodrigo; Pereira,Fabrícia Paniago Ajala Nery; Silveira,Ingridhy Ostaciana Maia Freitas da; Lima,Ricardo Vieira de; Berno,Claudia Rodrigues; Pesarini,João Renato; Antoniolli-Silva,Andréia Conceição Milan Brochado; Monreal,Antônio Carlos Duenhas; Adilson,Beatriz; Lima,Dênis Pires de; Gomes,Roberto da Silva.
Abstract Despite rapid advances in both the early detection and treatment of cancer, the mortality from this disease remains high, which justifies the development of new products that are more selective and effective and have fewer side effects. Accordingly, a novel ester was synthesized that contains two pharmacophores with important biological activities: (I) 4-aminoantipyrine, which has anti-inflammatory and antioxidant effects, and (II) the pharmacophore 1,4-dioxo-butenyl, which has cytotoxic activity. When administered alone, this compound is non-genotoxic, and it does not cause an increasing in splenic phagocytosis. Nevertheless, it can induce cell death. When administered in combination with commercial chemotherapeutic agents, such as doxorubicin,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer 4-aminoantipyrine 1; 4-dioxo-butenyl chemoprevention chemotherapy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300399

Analysis of castor bean ribosome-inactivating proteins and their gene expression during seed development
Provedor de dados: Genetics and Molecular Biology
Autores: Loss-Morais,Guilherme; Turchetto-Zolet,Andreia Carina; Etges,Matheus; Cagliari,Alexandro; Körbes,Ana Paula; Maraschin,Felipe dos Santos; Margis-Pinheiro,Márcia; Margis,Rogério.
Ribosome-inactivating proteins (RIPs) are enzymes that inhibit protein synthesis after depurination of a specific adenine in rRNA. The RIP family members are classified as type I RIPs that contain an RNA-N-glycosidase domain and type II RIPs that contain a lectin domain (B chain) in addition to the glycosidase domain (A chain). In this work, we identified 30 new plant RIPs and characterized 18 Ricinus communis RIPs. Phylogenetic and functional divergence analyses indicated that the emergence of type I and II RIPs probably occurred before the monocot/eudicot split. We also report the expression profiles of 18 castor bean genes, including those for ricin and agglutinin, in five seed stages as assessed by quantitative PCR. Ricin and agglutinin were the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agglutinin; Evolution; Lipase; Ricinus communis; Ricin; RIPs.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100011

Sequence characterization of coding regions of the myostatin gene (GDF8) from Brazilian Murrah buffaloes (Bubalus bubalis) and comparison with the Bos taurus sequence
Provedor de dados: Genetics and Molecular Biology
Autores: Mota,Lígia S.L.S.; Curi,Rogério A.; Palmieri,Darío A.; Borges,Alexandre S.; Lopes,Catalina R.; Barbosa,José D.; Gimenes,Marcos A.
Within about 30 years the Brazilian buffalo (Bubalus bubalis) herd will reach approximately 50 million head as a result of the great adaptive capacity of these animals to tropical climates, together with the good productive and reproductive potential which make these animals an important animal protein source for poor and developing countries. The myostatin gene (GDF8) is important in the physiology of stock animals because its product produces a direct effect on muscle development and consequently also on meat production. The myostatin sequence is known in several mammalian species and shows a high degree of amino acid sequence conservation, although the presence of non-silent and silent changes in the coding sequences and several alterations in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buffalo; GDF8; Myostatin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100016

ERRATA
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/article
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500025

Effects of APOE, APOB and LDLR variants on serum lipids and lack of association with xanthelasma in individuals from Southeastern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Nakazone,Marcelo A.; De Marchi,Miguel A.; Pinhel,Marcela A.S.; Barros,Carolina F.D.C.; Júlio,Maysa A.F.; Pinheiro,Anielli; Arazi,Simone S.; Hotta,Júlia K.; Hirata,Mário H.; Hirata,Rosario D.C.; Santos,José E. dos; Souza,Dorotéia R.S.
Xanthelasma might be a clinical manifestation of dyslipidemia, a recognized risk factor for coronary artery disease. We investigated the association of apolipoprotein E (APOE HhaI), apolipoprotein B (APOB XbaI and Ins/Del) and LDL receptor (LDLR AvaII and HincII) gene polymorphisms with lipid profiles in 100 Brazilians with xanthelasma and 100 controls. Allele frequencies were similar in both groups. APOE, APOB and LDLR genotypes were not correlated with differences in the serum lipid profile. In individuals with xanthelasma, the APOB D allele was associated with less chance of having increased LDL-cholesterol (O.R. = 0.16, CI95% = 0.03-0.94, p = 0.042). In the control group, the APOB X+ allele was associated with less chance of having both increased total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein B; Apolipoprotein E; LDL receptor; Gene polymorphisms; Xanthelasma.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200004

DNA repair diseases: what do they tell us about cancer and aging?
Provedor de dados: Genetics and Molecular Biology
Autores: Menck,Carlos FM; Munford,Veridiana.
The discovery of DNA repair defects in human syndromes, initially in xeroderma pigmentosum (XP) but later in many others, led to striking observations on the association of molecular defects and patients' clinical phenotypes. For example, patients with syndromes resulting from defective nucleotide excision repair (NER) or translesion synthesis (TLS) present high levels of skin cancer in areas exposed to sunlight. However, some defects in NER also lead to more severe symptoms, such as developmental and neurological impairment and signs of premature aging. Skin cancer in XP patients is clearly associated with increased mutagenesis and genomic instability, reflecting the defective repair of DNA lesions. By analogy, more severe symptoms observed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xeroderma pigmentosum; Ultraviolet; DNA damage; Oxidative stress; DNA repair.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200008

Genetic machinery for RNA silencing and defense against viruses in Citrus
Provedor de dados: Genetics and Molecular Biology
Autores: Benedito,Vagner Augusto; Faria,Laura; Freitas-Astúa,Juliana; Figueira,Antonio.
RNA silencing mechanisms are conserved throughout eukaryotic evolution, possibly due to their importance in viral resistance and other aspects of cell biology. Here, we explored the Citrus EST (CitEST) database in search of sequences related to the most important known genes involved in RNA silencing. Transcripts strongly matching Argonaute (AGO), Dicer-like (DCL), Hua enhancer (HEN), and RNA-dependent RNA Polymerase (RdRP) were found in many of the citrus libraries. The reads were clustered and quantified. This shows that post-transcriptional gene silencing apparatus is active in citrus. It seems plausible that a better understanding of the players of RNA silencing in Citrus spp. and related genera may help create new tools to defeat the viral diseases...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CitEST; Gene silencing; PTGS.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500027

Combined association of Presenilin-1 and Apolipoprotein E polymorphisms with maternal meiosis II error in Down syndrome births
Provedor de dados: Genetics and Molecular Biology
Autores: Bhaumik,Pranami; Ghosh,Priyanka; Ghosh,Sujay; Feingold,Eleanor; Ozbek,Umut; Sarkar,Biswanath; Dey,Subrata Kumar.
Abstract Alzheimer's disease and Down syndrome often exhibit close association and predictively share common genetic risk-factors. Presenilin-1 (PSEN-1) and Apolipoprotein E (APOE) genes are associated with early and late onset of Alzheimer's disease, respectively. Presenilin −1 is involved in faithful chromosomal segregation. A higher frequency of the APOE ε4 allele has been reported among young mothers giving birth to Down syndrome children. In this study, 170 Down syndrome patients, grouped according to maternal meiotic stage of nondisjunction and maternal age at conception, and their parents were genotyped for PSEN-1 intron-8 and APOE polymorphisms. The control group consisted of 186 mothers of karyotypically normal children. The frequencies of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome; Genetic polymorphism; Karyotype; Meiosis; Microsatellite markers.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400577

Histone deacetylase inhibitors as potential treatment for spinal muscular atrophy
Provedor de dados: Genetics and Molecular Biology
Autores: Mohseni,Jafar; Zabidi-Hussin,Z.A.M.H.; Sasongko,Teguh Haryo.
Histone acetylation plays an important role in regulation of transcription in eukaryotic cells by promoting a more relaxed chromatin structure necessary for transcriptional activation. Histone deacetylases (HDACs) remove acetyl groups and suppress gene expression. HDAC inhibitors (HDACIs) are a group of small molecules that promote gene transcription by chromatin remodeling and have been extensively studied as potential drugs for treating of spinal muscular atrophy. Various drugs in this class have been studied with regard to their efficacy in increasing the expression of survival of motor neuron (SMN) protein. In this review, we discuss the current literature on this topic and summarize the findings of the main studies in this field.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HDACi; Molecular therapy; Spinal muscular atrophy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300001

Absence of the -116A variant of the butyrylcholinesterase BCHE gene in Guarani Amerindians from Mato Grosso do Sul
Provedor de dados: Genetics and Molecular Biology
Autores: Nunes,Kelly; Souza,Ricardo L.R.; Furtado-Alle,Lupe; Tsuneto,Luiza T.; Petzl-Erler,Maria Luiza; Chautard-Freire-Maia,Eleidi A.
Butyrylcholinesterase (BChE; EC 3.1.1.8; Online Mendelian Inheritance in Man (OMIM) number 177400) is an enzyme found in many human tissues and encoded by the BCHE gene, of which 65 variants have been identified. In a recent study we found that the -116A variant of exon 1 of the BCHE gene was associated with lower mean BChE activity. The present study analyzed the -116 single nucleotide polymorphism (SNP) in 253 Guarani Amerindian Brazilians from the state of Mato Grosso do Sul (148 Guarani-Kaiowá, 83 Guarani-Ñandeva and 22 Kaiowá-Ñandeva descendants) and verified that they were all homozygotic for the -116G variant. A comparative analysis of the -116 site in nine vertebrate species indicated the -116A variant as the ancestral type. This is the first study...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Amerindians; BCHE gene; Guarani-Kaiowá; Guarani-Ñandeva.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100004

Analysis of genetic variability in three species of Pimelodidae (Ostariophysi - Siluriformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Almeida,Fernanda S. de; Sodré,Leda M.K.
Genetic variability of three Pimelodidae species, Pimelodus maculatus, Iheringichthys labrosus, and Pinirampus pirinampu, collected at one site in the Tibagi River, was comparatively analyzed using protein data for six systems which code 15 loci in liver, muscle, and heart. The proportion of polymorphic loci (&lt;img src="http:/img/fbpe/gmb/v21n4/21n4a14.jpg" alt="21n4a14.jpg (768 bytes)" align="middle"&gt;) for P. maculatus, I. labrosus, and P. pirinampu was 13.33, 20, and 6.67%, respectively, and mean heterozigosity was 6, 8.3, and 4.3%. The genetic identity value (I) was 0.32 between P. maculatus and I. labrosus, 0.37 between P. maculatus and P. pirinampu, and 0.58 between I. labrosus and P. pirinampu. This value suggests that these two latter species...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400014

Correlated responses to selection for large body size in oMt1a-oGH transgenic mice: organ traits
Provedor de dados: Genetics and Molecular Biology
Autores: Parks,K.R.; Eisen,E.J.; Murray,J.D.
The objective of the present study was to compare correlated responses in liver, spleen, kidney, heart and testis absolute weights and as a percentage of 8-week body weight following selection for large 8-week body weight in twice-replicated nontransgenic and transgene-carrier lines of mice from two genetic backgrounds. The transgene was an ovine metallothionein 1a-ovine growth hormone (oMt1a-oGH) construct, which was activated by adding 25 mM ZnSO4 to the drinking water. Lines NM and NC were nontransgenic lines derived from a high-growth and randomly selected background, respectively. Lines TM and TC were transgene-carrier lines formed from the respective genetic backgrounds. Line CC was a nontransgenic control from the randomly selected background. At...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400011

The influence of animals from embryo transfer on the genetic evaluation of growth in Simmental beef cattle by using multi-trait models
Provedor de dados: Genetics and Molecular Biology
Autores: Mota,Rodrigo Reis; Lopes,Paulo Sávio; Marques,Luiz Fernando Aarão; Silva,Luciano Pinheiro da; Resende,Marcos Deon Vilela de; Torres,Robledo de Almeida.
The weight records from Simmental beef cattle were used in a genetic evaluation of growth with or without the inclusion of animals obtained by embryo transfer. A multi-trait model in which embryo transfer individuals were excluded (MTM1) contained 29,510 records from 10,659 animals, while another model without exclusion of these animals (MTM2) contained 62,895 weight records from 23,160 animals. The weight records were adjusted for ages of 100, 205, 365, 450, 550 and 730 days. The (co)variance components and genetic parameters were estimated by the restricted maximum likelihood method. The (co)variance components were similar in both models, except for maternal permanent environment variance. Direct heritabilities (h²d) in MTM1 were 0.04, 0.11, 0.20, 0.27,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Body weight; (co)variance components; Heritability.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100007

Clinical and molecular analysis of the enamelin gene ENAM in Colombian families with autosomal dominant amelogenesis imperfecta
Provedor de dados: Genetics and Molecular Biology
Autores: Gutiérrez,Sandra; Torres,Diana; Briceño,Ignacio; Gómez,Ana Maria; Baquero,Eliana.
In this study, we analyzed the phenotype, clinical characteristics and presence of mutations in the enamelin gene ENAM in five Colombian families with autosomal dominant amelogenesis imperfecta (ADAI). 22 individuals (15 affected and seven unaffected) belonging to five Colombian families with ADAI and eight individuals (three affected and five unaffected) belonging to three Colombian families with autosomal recessive amelogenesis imperfecta (ARAI) that served as controls for molecular alterations and inheritance patterns were studied. Clinical, radiographic and genetic evaluations were done in all individuals. Eight exons and three intron-exon boundaries were sequenced for mutation analysis. Two of the five families with ADAI had the hypoplasic phenotype,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amelogenesis imperfecta; ENAM gene; Hypocalcified; Hypoplasic; Phenotype.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400003

Study of the CCR5-m303 mutation in three different ethnic groups from Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Grimaldi,Rogério; Acosta,Angelina Xavier; Cabral-Oliveira,Fernando Cézar; Brites,Carlos; Galvão-Castro,Bernardo.
The main coreceptor gene involved in HIV-1 infection is CCR5 beta chemokine receptor gene for which several mutations have been described, some of which have correlated with HIV-1 infection, acquired immune deficiency syndrome (AIDS), or both. Deletion of 32bp in the CCR5 gene (delta32) has been shown to confer resistance to infection by HIV-1 R5 strains. Another mutation, characterized by a thymine to adenine (T to A) nucleotide substitution at position 303 (m303), has shown the same effects as the delta32 mutation, with previous studies having shown that the allele frequency of the CCR5-m303 mutation is 0.014 in African-American and 0.007 in French populations. The Brazilian population is known to be genetically diverse, because of which we investigated...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CCR5 gene; M303 frequency; Brazilian populations; HIV-1.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200006

Protective effect of Hemidesmus indicus R.Br. root extract against cisplatin-induced cytogenetic damage in mouse bone marrow cells
Provedor de dados: Genetics and Molecular Biology
Autores: Ananthi,Rajagopal; Chandra,Nallathambi; Santhiya,Sathiyavedu T.
The aqueous extract of Hemidesmus indicus roots was investigated for its in vivo antigenotoxic effect against cisplatin-induced cytogenetic damage. Swiss albino mice were administered with various doses of the extract either singly (50, 100 and 200 mg/kg body weight) or as split doses (10, 20 and 40 mg/kg bw/day) for five consecutive days by oral gavage. As endpoints, chromosome aberrations, micronuclei in polychromatic erythrocytes, mitotic index and PCE/NCE ratio were estimated. The extract protected the bone marrow cells from cisplatin-induced genotoxicity in an inverse dose-dependent manner. However, the extract was cytotoxic at all doses. But, under split dose regime it conferred a higher level of genoprotection and was not cytotoxic at the lower two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genoprotective; Cytotoxic; Chromosomal aberrations; Micronucleus; Mitotic index.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100030

NCAPG is differentially expressed during longissimus muscle development and is associated with growth traits in Chinese Qinchuan beef cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Liu,Yu; Duan,Xiaoyan; Chen,Si; He,Hua; Liu,Xiaolin.
Abstract Based on RNA-seq analysis, we recently found that the bovine NCAPG (non-SMC condensin I complex, subunit G) gene is differentially expressed during development of the longissimus muscle. In the present study, we validated this result and, using quantitative real-time PCR analysis, identified two adjacent genes, LCORL and DCAF16, that are more abundant in fetal muscle tissue; further analysis of tissue-specific expression patterns indicated high abundance of NCAPG in muscle. Since no polymorphisms were detected in a previous study of Qinchuan cattle, we extended our investigation to examine the occurrence of single-nucleotide polymorphisms (SNPs) in the NCAPG gene. Three SNPs, i.e., one located in the intron region (g47747: T &gt; G), a synonymous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Association analysis; Growth traits; Longissimus muscle; NCAPG expression; Single nucleotide polymorphism.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400450

Microsatellites retain phylogenetic signals across genera in eucalypts (Myrtaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Ochieng,Joel W.; Steane,Dorothy A.; Ladiges,Pauline Y.; Baverstock,Peter R.; Henry,Robert J.; Shepherd,Mervyn.
The utility of microsatellites (SSRs) in reconstructing phylogenies is largely confined to studies below the genus level, due to the potential of homoplasy resulting from allele size range constraints and poor SSR transferability among divergent taxa. The eucalypt genus Corymbia has been shown to be monophyletic using morphological characters, however, analyses of intergenic spacer sequences have resulted in contradictory hypotheses- showing the genus as either equivocal or paraphyletic. To assess SSR utility in higher order phylogeny in the family Myrtaceae, phylogenetic relationships of the bloodwood eucalypts Corymbia and related genera were investigated using eight polymorphic SSRs. Repeat size variation using the average square and Nei's distance were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite phylogeny; Paraphyletic; Homoplasy; Incongruence; Eucalypts.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600016

Isozyme variation in four species of the Simulium perflavum species group (Diptera: Simuliidae) from the Brazilian Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Scarpassa,Vera Margarete; Hamada,Neusa.
Electrophoretic studies of isozymes were done with four closely related species of the Simulium perflavum species group (Diptera: Simuliidade) in the Brazilian Amazon, using last-instar larvae collected in the field. Ten enzymes were studied, which yielded 11 loci. Diagnostic loci were not found between Simulium maroniense cytotype D and Simulium rorotaense. Simulium maroniense and S. rorotaense differed from Simulium trombetense by two diagnostic loci (Me and Xdh), and Simulium perflavum differed from the other three species by four diagnostic loci (Me, Xdh, Mdh, and Got). The mean number of alleles per locus ranged from 1.30 to 2.30, the percentage of polymorphic loci ranged from 18.2 to 63.6% and the mean heterozygosity values observed ranged from 0.062...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Simulium perflavum species group; Isozymes; Genetic divergence; Biochemical systematic; Brazilian Amazon.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100007

Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Jéssica Barata da; Suárez,Pablo; Nagamachi,Cleusa Yoshiko; Carter,Timothy Frederick; Pieczarka,Julio Cesar.
Plethodontid salamanders of genus Bolitoglossa constitute the largest and most diverse group of salamanders, including around 20% of living caudate species. Recent studies have indicated the occurrence of five recognized species in the Brazilian Amazon Rainforest. We present here the first cytogenetic data of a Brazilian salamander, which may prove to be a useful by contribution to the cytotaxonomy of the genus. Specimens were collected near the "type" locality (Utinga, Belém, PA, Brazil). Chromosomal preparations from duodenal epithelial cells and testes were subjected to Giemsa staining, C-banding and DAPI/CMA3 fluorochrome staining. All specimens showed a karyotype with 13 bi-armed chromosome pairs (2n = 26). Nucleolar Organizer Regions, evidenced by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salamanders; Bolitoglossa; Karyotypes; Brazilian Amazon; Cytogenetics.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400008

An alkaline single-cell gel electrophoresis (comet) assay for environmental biomonitoring with native rodents
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Juliana da; Freitas,Thales R.O. de; Marinho,Jorge R.; Speit,Günter; Erdtmann,Bernardo.
The main advantages of single-cell gel electrophoresis (SCG) are its applicability to any eukaryotic organism and cell type, its low cost and the short time required to obtain results. These properties make the SCG assay particularly useful in screening for environmental genotoxicity. The present study describes a modified version of this technique for use in field work with native rodents and examines some factors which influence the outcome of the assay. Wild rodents (Ctenomys torquatus, "tuco-tuco") from a region close to a strip coal mine and from a region with no coal mines were used. Animals from the coal mining region had significantly more DNA damage than those from the control area. The use of this SCG technique for direct sampling in the field...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100042

TaqI B1/B2 and -629A/C cholesteryl ester transfer protein (CETP) gene polymorphisms and their association with CETP activity and high-density lipoprotein cholesterol levels in a Tehranian population. Part of the Tehran Lipid and Glucose Study (TLGS)
Provedor de dados: Genetics and Molecular Biology
Autores: Daneshpour,Maryam S; Hedayati,Mehdi; Azizi,Fereidoun.
We examined the cholesteryl ester transfer protein (CETP) gene TaqI intron 1 B1/B2 polymorphism and the -629A/C CETP promoter polymorphism in respect to high-density lipoprotein cholesterol (HDL-C) in a healthy Iranian population taken from the Tehran Lipid and Glucose Study (TLGS). The relationship between CETP activity and HDL-C level was also determined along with body mass index, blood pressure and tobacco smoking status. PCR-RFLP used to amplify a segment of the CETP intron 1 TaqI (B2/B1) polymorphism from 1021 individuals and we selected 345 individuals from the lowest, middle and highest HDL-C deciles and investigated the -629A/C polymorphism. We also evaluated the CETP activity of 103 of these individuals, each with at least one homozygous allele....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholesteryl ester transfer protein (CETP); Hardy-Weinberg equilibrium; Linkage disequilibrium; Polymorphisms.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600001

PCR screening and allele frequency estimation of bovine leukocyte adhesion deficiency in Holstein and Gir cattle in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Ribeiro,Luciana A.; Baron,Erica E.; Martinez,Mário L.; Coutinho,Luiz L.
Bovine leukocyte adhesion deficiency (BLAD) is a disease known to affect the Holstein cattle breed throughout the world. Eighty-eight Holstein dairy cows and 88 Gir dairy bulls were genotyped by PCR for the CD18 BLAD alelle. The frequency of the BLAD mutant allele and the BLAD-carrier prevalence in Brazilian Holstein cows were 2.8 and 5.7%, respectively. No mutant allele was found in any of the 88 Gir animals. We conclude that the CD18 gene mutation is prevalent in Brazilian Holstein cattle and absent or present at a very low frequency in Gir cattle.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400021

Cytogenetic analysis of five species of the subfamily Corydoradinae (Teleostei: Siluriformes: Callichthyidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Shimabukuro-Dias,Cristiane Kioko; Oliveira,Claudio; Foresti,Fausto.
In the present study, five callichthyid species belonging to the subfamily Corydoradinae were karyotyped: three species of Aspidoras and two of Corydoras. The three species of Aspidoras had the same diploid number, 2n = 46 chromosomes, similar karyotypic formulae, with most chromosomes metacentric or submetacentric, single interstitial Ag-NORs and C-band positive segments mainly found in the centromeric position. The comparative analysis of cytogenetic data available for the genus Aspidoras and other species of Corydoradinae suggest that several events of centric fusion occurred in the origin of the species of Aspidoras. The two analyzed species of Corydoras showed high diploid numbers, 2n = 74 in C. sodalis and 2n = 90 in C. britskii. While C. sodalis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish cytogenetic; Karyotype; NOR; C-banding; Karyotypic evolution.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400014

Preimplantation genetic diagnosis of Von Hippel-Lindau disease cancer syndrome by combined mutation and segregation analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Sumita,Denilce R.; Rocha,José Cláudio C.; Iaconelli Jr.,Assumpto; Borges Jr.,Edson; Pereira,Lygia V.
Von Hippel-Lindau (VHL) disease is an autosomal dominant cancer syndrome, associated with the development of tumors and cysts in multiple organ systems, whose expression and age of onset are highly variable. The VHL disease tumor suppressor gene (VHL) maps to 3p25-p26 and mutations ranging from a single base change to large deletions have been detected in patients with VHL disease. We developed a single cell PCR protocol for preimplantation genetic diagnosis (PGD) of VHL disease to select unaffected embryos on the basis of the detection of the specific mutation and segregation analysis of polymorphic linked markers. Multiplex-nested PCR using single buccal cells of an affected individual were performed in order to test the accuracy and reliability of this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiplex PCR; Preimplantation genetic diagnosis; Von Hippel-Lindau disease; VHL gene.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300005

The complete mitochondrial genome of the pirarucu (Arapaima gigas, Arapaimidae, Osteoglossiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Hrbek,Tomas; Farias,Izeni Pires.
We sequenced the complete mitochondrial genome of the pirarucu, Arapaima gigas, the largest fish of the Amazon basin, and economically one of the most important species of the region. The total length of the Arapaima gigas mitochondrial genome is 16,433 bp. The mitochondrial genome contains 13 protein-coding genes, two rRNA genes and 22 tRNA genes. Twelve of the thirteen protein-coding genes are coded on the heavy strand, while nad6 is coded on the light strand. The Arapaima gene order and content is identical to the common vertebrate form, as is codon usage and base composition. Its control region is atypical in being short at 767 bp. The control region also contains a conserved ATGTA motif recently identified in the Asian arowana, three conserved...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pirarucu; Mitogenomics; Amazon basin.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200024

Mitochondrial DNA variation in wild populations of Leporinus elongatus from the Paraná River basin
Provedor de dados: Genetics and Molecular Biology
Autores: Martins,Cesar; Wasko,Adriane P.; Oliveira,Claudio; Foresti,Fausto.
Leporinus elongatus, a fish species widely distributed throughout the Paraná River basin in South America, is an important fishery resource and a valuable species in aquaculture programs. Despite its great economic importance, several wild populations have been suffering a drastic reduction. The comprehension of its population structure represents an important step for the conservation of these organisms in natural environments, and also for the selection of wild stocks to be used in hatchery programs. In order to understand the genetic-population structure of L. elongatus, D-loop mitochondrial DNA analyses were applied in six wild populations of the species. The results were used to estimate the levels of within and among population genetic variability....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; D-loop; Genetic variability; Leporinus elongatus; MtDNA.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100006

Molecular identification of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila
Provedor de dados: Genetics and Molecular Biology
Autores: Mota,Adolfo Jose; Back-Brito,Graziella Nuernberg; Nobrega,Francisco G.
Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was validated by DNA sequencing of the polymorphic region used in a real-time PCR assay driven by species-specific probes targeted to the fungal ITS 1 region. This assay provides a new tool for pathogen identification and for epidemiological, drug resistance and virulence studies of these organisms.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida palmioleophila; Debaryomyces hansenii; Differential identification; Pichia guilliermondii; Real-time PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100017

Skewed RAPD markers in linkage maps of Citrus
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Roberto Pedroso de; Aguilar-Vildoso,Carlos Ivan; Cristofani,Mariângela; Machado,Marcos Antônio.
The objective of this work was to analyze the effects of RAPD markers with skewed segregation on genetic linkage maps. Segregation data for 123 Citrus sinensis (L.) Osbeck cv. Pêra markers and 53 C. reticulata Blanco cv. Cravo markers in F1 progeny composed of 94 hybrids were used. Genetic linkage maps of the two varieties were constructed with non-skewed markers (p &lt; 0.05 and p &lt; 0.01) using the program MAPMAKER 3.0 and a pseudo-testcross strategy. The maps were compared to those constructed with all markers. Alterations in the genetic distances were observed based on the location of the skewed markers within the linkage groups. Generally, the skewed markers were located at the end of the linkage groups, sometimes forming entire linkage groups,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic distance; Mendelian segregation; Molecular markers; Segregation distortion.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300021

Comparative cytogenetics of nine populations of the Rhinella genus (Anura, Bufonidae) with a highlight on their conservative karyotype
Provedor de dados: Genetics and Molecular Biology
Autores: Bruschi,Daniel Pacheco; Sousa,Deborah Yasmim; Soares,Amanda; Carvalho,Klélia Aparecida de; Busin,Carmen Sílvia; Ficanha,Natália Cristine; Lima,Albertina Pimentel; Andrade,Gilda Vasconcellos; Recco-Pimentel,Shirlei Maria.
Abstract The genus Rhinella is one of the most diverse groups of bufonid toads, currently composed by 93 valid species and naturally distributed throughout different Neotropical ecoregions. Here, we analyze nine Brazilian populations of toads representing species of the Rhinella margaritifera and Rhinella marina groups. These new data include the first description of the R. hoogmoedi and R. proboscidae karyotypes, as well as other taxonomically unresolved forms. Chromosomal analysis of the populations revealed pronounced chromosomal uniformity (2n=22), including the diploid number and chromosomal morphology. Three different NOR-bearing chromosomes were identified: in the subterminal region of pair 10q in R. hoogmoedi, Rhinella sp. 1 and Rhinella sp. 2, in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bufonid toads; Nucleolar organizing region; Chromosomal evolution.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300445

Purine and pyrimidine composition in 5S rRNA and its mutational significance
Provedor de dados: Genetics and Molecular Biology
Autores: Subacius,Sandra Maria Rodrigues; Bussab,Wilton de Oliveira.
Variations observed in 5S rRNA base compositions are almost entirely due to fixation of point mutations. As a consequence, 5S rRNA size has remained relatively constant during evolution and, therefore, dependencies among the four bases can be predicted. In order to characterize the nature and to determine the degree of such dependencies, correlation analysis followed by principal component factorial analysis was conducted on a large sample of 5S rRNA sequences. The results show that the purine and pyrimidine contents tend to remain constant, so that A + G = Kpur and C + U = Kpyr. The composition of the four bases expressed now by Kpur/Kpyr relationships is also constant (Ks). These relationships imply that the behavior of the mutations in the variable...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200014

A deterministic simulation study of embryo marker-assisted selection for age at first calving in Nellore (Bos indicus) beef cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Rosa,Artur J.M.; Bijma,Piter; Oliveira,Henrique N.; Lôbo,Raysildo B.; van Arendonk,Johan A.M.
We used deterministic simulation of four alternative multiple ovulation and embryo manipulation (MOET) closed nucleus schemes to investigate the benefits of using marker-assisted selection (MAS) of Nellore (Bos indicus) beef cattle embryos prior to transplantation to reduce the age at first calving (AFC). We found that MAS resulted in increased genetic gain as compared to selection without AFC quantitative trait loci (AFC-QTL) information. With single-stage selection the genetic response (GR) increased as follows: GR = 0.68% when the AFC-QTL explained 0.02 of the AFC additive genetic variance (sigma2A); GR = 1.76% for AFC-QTL explaining 0.05 sigma2A; GR = 3.7% for AFC-QTL explaining 0.1 sigma2A; and GR = 55.76% for AFC-QTL explaining 0.95 sigma2A. At the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Age at first calving; Beef cattle; Marker-assisted selection; Pre-selection; Quantitative trait loci.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400008

Type 2 diabetes associated variants of KCNQ1 strongly confer the risk of cardiovascular disease among the Saudi Arabian population
Provedor de dados: Genetics and Molecular Biology
Autores: Al-Shammari,Maha S.; Al-Ali,Rhaya; Al-Balawi,Nader; Al-Enazi,Mansour S.; Al-Muraikhi,Ali A.; Busaleh,Fadi N.; Al-Sahwan,Ali S.; Al-Elq,Abdulmohsen; Al-Nafaie,Awatif N.; Borgio,Jesu Francis; AbdulAzeez,Sayed; Al-Ali,Amein; Acharya,Sadananda.
Abstract Genome-wide association studies have identified several loci associated with an increased risk for cardiovascular disease (CVD) and type 2 diabetes (T2D). Polymorphisms within the KCNQ1 (potassium voltage-gated channel, KQT-like subfamily, member 1) gene are consistently associated with T2D in a number of populations. The current study was undertaken to evaluate the association of 3 polymorphisms of KCNQ1 (rs2237892, rs151290 and rs2237895) with T2D and/or CVD. Patients diagnosed with either T2D (320 patients), CVD (250 patients) or both (60 patients) and 516 healthy controls were genotyped by TaqMan assay run on a real time PCR thermocycler. A statistically significant association was found for SNPs rs151290 (OR = 1.76; 95%CI = 1.02-3.05; p =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: T2D; CVD; KCNQ1; Genetic association; Saudi population.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400586

Frequencies of X-ray induced chromosome aberrations in lymphocytes of xeroderma pigmentosum and Fanconi anemia patients estimated by Giemsa and fluorescence in situ hybridization staining techniques
Provedor de dados: Genetics and Molecular Biology
Autores: Saraswathy,Radha; Natarajan,A.T.
Blood lymphocytes from xeroderma pigmentosum (XP) and Fanconi anemia (FA) patients were assessed for their sensitivity to ionizing radiation by estimating the frequency of X-ray (1 and 2 Gy)-induced chromosome aberrations (CA). The frequencies of aberrations in the whole genome were estimated in Giemsa-stained preparations of lymphocytes irradiated at G0 or G2 stages. The frequencies of translocations and dicentrics involving chromosomes 1 and 3 as well as the X-chromosome were determined in slides stained by fluorescence in situ hybridization (FISH) technique. An increase in all types of CA was observed in XP and FA lymphocytes irradiated at G0 when compared to controls. The frequency of dicentrics and rings was 6 to 27% higher (at 1 and 2 Gy) in XP...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400031

Neuromuscular disorders: genes, genetic counseling and therapeutic trials
Provedor de dados: Genetics and Molecular Biology
Autores: Zatz,Mayana; Passos-Bueno,Maria Rita; Vainzof,Mariz.
Abstract Neuromuscular disorders (NMD) are a heterogeneous group of genetic conditions, with autosomal dominant, recessive, or X-linked inheritance. They are characterized by progressive muscle degeneration and weakness. Here, we are presenting our major contributions to the field during the past 30 years. We have mapped and identified several novel genes responsible for NMD. Genotype-phenotype correlations studies enhanced our comprehension on the effect of gene mutations on related proteins and their impact on clinical findings. The search for modifier factors allowed the identification of a novel "protective"; variant which may have important implication on therapeutic developments. Molecular diagnosis was introduced in the 1980s and new technologies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diseases; Genetic counseling; Neuromuscular disorders; Stem cells; Therapies.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300339

Muscle biopsy technique for electrophoresis analysis of fish from the genus Brycon
Provedor de dados: Genetics and Molecular Biology
Autores: Hilsdorf,Alexandre; Caneppele,Danilo; Krieger,José Eduardo.
Protein and mitochondrial DNA have been used as molecular markers to assess variability in stock identification studies of fishes. Protein and mtDNA used for electrophoretic analysis are extracted from tissues, which often leads to death of the individuals. In this study, we present a skeletal muscle biopsy procedure to extract mitochondrial DNA that does not require specimen sacrifice. Eighty pirapitinga-do-sul (Brycon opalinus) were biopsied by the present technique, with no mortalities recorded. Total DNA was extracted from muscle and digested by restriction enzymes ApaI and HpaI. The mtDNA fragment patterns were hybridized with 32P-labeled pirapitinga-do-sul mtDNA probes. The described technique is simple and may be useful in protocols requiring tissue...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400014

Genetic diversity in a world germplasm collection of tall fescue
Provedor de dados: Genetics and Molecular Biology
Autores: Cuyeu,Romina; Rosso,Beatriz; Pagano,Elba; Soto,Gabriela; Fox,Romina; Ayub,Nicolás Daniel.
Festuca arundinacea Schreb., commonly known as tall fescue, is a major forage crop in temperate regions. Recently, a molecular analysis of different accessions of a world germplasm collection of tall fescue has demonstrated that it contains different species from the genus Festuca and allowed their rapid classification into the three major morphotypes (Continental, Mediterranean and Rhizomatous). In this study, we explored the genetic diversity of 161 accessions of Festuca species from 29 countries, including 28 accessions of INTA (Argentina), by analyzing 15 polymorphic SSR markers by capillary electrophoresis. These molecular markers allowed us to detect a total of 214 alleles. The number of alleles per locus varied between 5 and 24, and the values of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR markers; Genetic diversity; Flow cytometry; Tall fescue; Genetic resources.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200014

Genotype by environment interaction for 450-day weight of Nelore cattle analyzed by reaction norm models
Provedor de dados: Genetics and Molecular Biology
Autores: Pégolo,Newton T.; Oliveira,Henrique N.; Albuquerque,Lúcia G.; Bezerra,Luiz Antonio F.; Lôbo,Raysildo B.
Genotype by environment interactions (GEI) have attracted increasing attention in tropical breeding programs because of the variety of production systems involved. In this work, we assessed GEI in 450-day adjusted weight (W450) Nelore cattle from 366 Brazilian herds by comparing traditional univariate single-environment model analysis (UM) and random regression first order reaction norm models for six environmental variables: standard deviations of herd-year (RRMw) and herd-year-season-management (RRMw-m) groups for mean W450, standard deviations of herd-year (RRMg) and herd-year-season-management (RRMg-m) groups adjusted for 365-450 days weight gain (G450) averages, and two iterative algorithms using herd-year-season-management group solution estimates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth; Genotype by environment interaction; Plasticity; Random regression; Robustness.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200013

Characterization of swine stress gene by DNA testing using plucked hair as a source of DNA
Provedor de dados: Genetics and Molecular Biology
Autores: Bastos,Reginaldo Gaspar; Federizzi,Joreci; Deschamps,João Carlos; Cardellino,Ricardo; Dellagostin,Odir Antônio.
The swine stress gene (hal) in recessive homozygotes (nn) leads to porcine stress syndrome (PSS), and is associated with pale, soft, exudative pork (PSE). In heterozygosis (Nn) it is linked to poor carcass quality. A total of 179 pigs (86 Large White, 69 Landrace, 12 Duroc and 12 Pietrain) were characterized as normal homozygotes (NN), heterozygotes or recessive homozygotes following amplification of a target region of the hal gene using the polymerase chain reaction (PCR), followed by a restriction endonuclease assay. Plucked hair was used as a source of genomic DNA. The resulting PCR was digested with the restriction enzyme CfoI, followed by agarose gel electrophoresis. Of 179 animals tested, 70% were NN, 28% were Nn, and 2% were nn. The frequency of...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400018

Centromeric, pericentromeric and heterochromatin abnormalities in chromosomal rearrangements of human leukemia
Provedor de dados: Genetics and Molecular Biology
Autores: Berger,Roland.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400060

Cytotaxonomy of Sapindaceae with special reference to the tribe Paullinieae
Provedor de dados: Genetics and Molecular Biology
Autores: Ferrucci,María S.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400039

A shortcut in phage screening technique
Provedor de dados: Genetics and Molecular Biology
Autores: Andrade,Alexandre de; Teixeira,Paula Rezende; Siviero,Fábio; Santelli,Roberto Vicente.
A simple modification of the traditional Benton &amp;amp; Davis technique for phage screening is presented that avoids the tedious sample dilutions of putative spots/phages towards the second screening. With the use of a sole agar plate and nylon filter, the modification distinguishes a true positive recombinant from a false positive, with high probability of success.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phage screening shortcut for Benton &amp;amp; Davis technique single agar-plate/nylon filter.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100025

Variability in micronucleus induction with different mutagens applied to several species of fish
Provedor de dados: Genetics and Molecular Biology
Autores: Grisolia,Cesar Koppe; Cordeiro,Célia Maria Torres.
Fish are often used for screening genotoxicity of water. For such programs, a knowledge of the sensitivity to clastogens, spontaneous micronucleus frequency and cell cycle kinetics of the target tissue is necessary. To investigate the pattern of inter-specific sensitivity to micronucleus induction three species of fish, Tilapia rendalli, Oreochromis niloticus and Cyprinus carpio, were exposed to the clastogens bleomycin (BLM), cyclophosphamide (CP), 5-fluorouracil (5-FU), and mitomycin C (MMC). The binucleate/mononucleate ratio in peripheral erythrocytes exposed to cytochalasin B was also used to evaluate the time-dependent response of micronucleus formation during hematopoesis in the kidney and the micronucleus peak in peripheral erythrocytes....
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100041

Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder
Provedor de dados: Genetics and Molecular Biology
Autores: Pljesa-Ercegovac,Marija; Savic-Radojevic,Ana; Kravic-Stevovic,Tamara; Bumbasirevic,Vladimir; Mimic-Oka,Jasmina; Simic,Tatiana.
Transitional cell carcinoma (TCC) of urinary bladder belongs to glutathione S-transferase P1 (GSTP1) overexpressing tumors. Upregulated GSTP1 in TCC is related to apoptosis inhibition. This antiapoptotic effects of GSTP1 might be mediated through protein:protein interaction with c-Jun NH2-terminal kinase (JNK). Herein, we analyzed whether a direct link between GSTP1 and JNK exists in TCC. The presence of GSTP1/JNK complexes was analyzed by immunoprecipitation and Western blotting in 20 TCC specimens, obtained after surgery. Co-localization of GSTP1 and JNK was also investigated in the 5637 TCC cell line by immunofluorescence confocal microscopy. By means of immunoprecipitation we show for the first time the presence of GSTP1/JNK complexes in all TCC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione S-transferase; JNK; TCC; Co-localization.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300013

A straightforward multiallelic significance test for the Hardy-Weinberg equilibrium law
Provedor de dados: Genetics and Molecular Biology
Autores: Lauretto,Marcelo S.; Nakano,Fabio; Faria Jr,Silvio R.; Pereira,Carlos A.B.; Stern,Julio M.
Much forensic inference based upon DNA evidence is made assuming Hardy-Weinberg Equilibrium (HWE) for the genetic loci being used. Several statistical tests to detect and measure deviation from HWE have been devised, and their limitations become more obvious when testing for deviation within multiallelic DNA loci. The most popular methods-Chi-square and Likelihood-ratio tests-are based on asymptotic results and cannot guarantee a good performance in the presence of low frequency genotypes. Since the parameter space dimension increases at a quadratic rate on the number of alleles, some authors suggest applying sequential methods, where the multiallelic case is reformulated as a sequence of "biallelic" tests. However, in this approach it is not obvious how...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hardy-Weinberg equilibrium; Significance tests; FBST.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300028

An overall evaluation of the resistance (R) and pathogenesis-related (PR) superfamilies in soybean, as compared with Medicago and Arabidopsis
Provedor de dados: Genetics and Molecular Biology
Autores: Wanderley-Nogueira,Ana C.; Belarmino,Luis C.; Soares-Cavalcanti,Nina da M.; Bezerra-Neto,João P.; Kido,Ederson A.; Pandolfi,Valesca; Abdelnoor,Ricardo V.; Binneck,Eliseu; Carazzole,Marcelo F.; Benko-Iseppon,Ana M.
Plants have the ability to recognize and respond to a multitude of pathogens, resulting in a massive reprogramming of the plant to activate defense responses including Resistance (R) and Pathogenesis-Related (PR) genes. Abiotic stresses can also activate PR genes and enhance pathogen resistance, representing valuable genes for breeding purposes. The present work offers an overview of soybean R and PR genes present in the GENOSOJA (Brazilian Soybean Genome Consortium) platform, regarding their structure, abundance, evolution and role in the plantpathogen metabolic pathway, as compared with Medicago and Arabidopsis. Searches revealed 3,065 R candidates (756 in Soybean, 1,142 in Medicago and 1,167 in Arabidopsis), and PR candidates matching to 1,261 sequences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogen response; Biotic stress; Bioinformatics; Glycine max; Medicago truncatula.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200007

Phylogenetic position of an uncharacterized Brazilian strain of bovine papillomavirus in the genus Xipapillomavirus based on sequencing of the L1 open reading frame
Provedor de dados: Genetics and Molecular Biology
Autores: Lunardi,Michele; Claus,Marlise P.; Alfieri,Amauri A.; Fungaro,Maria Helena P.; Alfieri,Alice F.
The use of PCR assays with degenerate primers has suggested the existence of numerous as yet uncharacterized bovine papillomaviruses (BPV). Despite the endemic nature of BPV infections, the identification of BPV types in Brazilian cattle is still only sporadic. However, in a recent analysis of a partial segment of the L1 gene, we observed notable diversity among the BPV types detected. The aim of this study was to determine the phylogenetic position of the previously identified wild strain BPV/BR-UEL2 detected in the state of Paraná in Brazil. Since previous analysis of the partial L1 sequence had shown that this strain was most closely related to BPV type 4, genus-specific primers were designed. Phylogenetic analysis using complete L1 ORF sequences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BPV; Cattle; Cutaneous papillomatosis; Putative new BPV type; L1 gene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400022

Bacteria associated with Amblyomma cajennense tick eggs
Provedor de dados: Genetics and Molecular Biology
Autores: Machado-Ferreira,Erik; Vizzoni,Vinicius Figueiredo; Piesman,Joseph; Gazeta,Gilberto Salles; Soares,Carlos Augusto Gomes.
Abstract Ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. In the Americas, the Ixodidae ticks Amblyomma cajennense are responsible for severe impact on livestock and public health. In the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with A. cajennense eggs from females sampled in distinct geographical sites in southeastern Brazil. Additional comparative analysis of the culturable bacteria from Anocentor nitens, Rhipicephalus sanguineus and Ixodes scapularis tick eggs were also performed. 16S rRNA gene sequence analyses identified 17 different bacterial types identified as Serratia marcescens, Stenotrophomonas maltophilia, Pseudomonas fluorescens,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amblyomma cajennense; Tick eggs bacteria; Staphylococcus; Ixodidae.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400477

GSTT1 and GSTM1 null variants in Mestizo and Amerindian populations from northwestern Mexico and a literature review
Provedor de dados: Genetics and Molecular Biology
Autores: Palma-Cano,Luz Elena; Córdova,Emilio J.; Orozco,Lorena; Martínez-Hernández,Angélica; Cid,Miguel; Leal-Berumen,Irene; Licón-Trillo,Angel; Lechuga-Valles,Ruth; González-Ponce,Mauricio; González-Rodríguez,Everardo; Moreno-Brito,Verónica.
Abstract The GSTT1 and GSTM1 genes are key molecules in cellular detoxification. Null variants in these genes are associated with increase susceptibility to developing different types of cancers. The aim of this study was to determine the prevalence of GSTT1 and GSTM1 null genotypes in Mestizo and Amerindian individuals from the Northwestern region of Mexico, and to compare them with those reported worldwide. GSTT1 and GSTM1 null variants were genotyped by multiplex PCR in 211 Mestizos and 211 Amerindian individuals. Studies reporting on frequency of GSTT1 and GSTM1 null variants worldwide were identified by a PubMed search and their geographic distribution were analyzed. We found no significant differences in the frequency of the null genotype for GSTT1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; GSTT1; GSTM1; Null variants.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500727

Age-age correlation for early selection of rubber tree genotypes in São Paulo State, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Paulo de Souza; Bortoletto,Nelson; Cardinal,Átila Bento Beleti; Gouvêa,Lígia Regina Lima; Costa,Reginaldo Brito da; Moraes,Mário Luiz Teixeira de.
In a field trial involving 68 rubber tree (Hevea spp.) clones calculation of genotypic correlation coefficients revealed significant age-age correlation from age 1 to 6 (immature period) for girth A and for age 7 to 12 (mature period) for girth B and for age 7 to 12 (production of latex) for yield. Rank correlation coefficients between all immature ages of girth (girth A), all ages of mature girth (girth B) and all annual rubber production (yield) were significant for the three traits, with the coefficients decreasing with increasing age. Selection of the sets of best 30, 15, 10 and 5 clones from the available 68 clones at a given age was generally accompanied by a descending order of percentage success. It was suggested: (a) to have the best 30 clones of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hevea brasiliensis; Early clonal multiplication; Genetic correlation; Rank correlation; Evaluation of clones.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500018

Article Molecular analysis of three FUT3 gene single nucleotide polymorphisms and their relationship with the lewis erythrocytary phenotype in a human population of japanese-ancestry living in Tomé Açu, a town in the Brazilian Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Francez,Pablo Abdon da Costa; Corvelo,Tereza Cristina de Oliveira; Silva,Flávio Ricardo Leal da; Santos,Sidney Emanuel Batista dos.
The Lewis blood group system involves two major antigens, Leª and Le b. Their antigenic determinants are not primary gene products but are synthesized by the transfer of sugar subunits to a precursory chain by a specific enzyme which is the product of the FUT3 gene (Lewis gene). The presence of three FUT3 gene single nucleotide polymorphisms (SNPs) (59T &gt; G; 508G &gt; A and 1067T &gt; A) was related to the Lewis phenotype of erythrocytes from 185 individuals of Japanese ancestry living in the town of Tomé-Açu in the Brazilian Amazon region. This relationship was detected using a serological hemagglutination test and the Dot-ELISA assay along with the molecular technique PCR-RFLP. We found that the three SNPs investigated in this study only accounted for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lewis blood groups; FUT3; Japanese; PCR-RFLPs; SNPs.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300002

The UCP2 -866G/A, Ala55Val and UCP3 -55C/T polymorphisms are associated with premature coronary artery disease and cardiovascular risk factors in Mexican population
Provedor de dados: Genetics and Molecular Biology
Autores: Gamboa,Ricardo; Huesca-Gómez,Claudia; López-Pérez,Vanessa; Posadas-Sánchez,Rosalinda; Cardoso-Saldaña,Guillermo; Medina-Urrutia,Aida; Juárez-Rojas,Juan Gabriel; Soto,María Elena; Posadas-Romero,Carlos; Vargas-Alarcón,Gilberto.
Abstract We examined the role of UCP gene polymorphisms as susceptibility markers for premature coronary artery disease (pCAD). The UCP2 Ala55Val (C/T rs660339), UCP2 -866G/A (rs659366), and UCP3 -55C/T (rs1800849) polymorphisms were genotyped in 948 patients with pCAD, and 763 controls. The distribution of the UCP2 A55V (C/T rs660339) and UCP3 -55 (rs1800849) was similar in patients and controls. However, under a recessive model, the UCP2 -866 (rs659366) A allele was associated with increased risk of developing pCAD (OR = 1.43, Pc = 0.003). On the other hand, patients with pCAD and UCP2 A55V (rs660339) TT showed high levels of visceral abdominal fat (VAF) (Pc = 0.002), low levels of subcutaneous abdominal fat (SAF) (Pc = 0.001) and high VAT/SAT ratio (Pc...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: UCPs polymorphisms; Premature coronary artery; Cardiovascular risk; Mexican population.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300371

Recurrence risks for isolated cases of nonsyndromic deafness
Provedor de dados: Genetics and Molecular Biology
Autores: Braga,Maria Cristina C.; Otto,Paulo A.
We present, in this paper, general formulae developed so as to permit the calculation of the recurrence risks for isolated cases of nonsyndromic deafness in the offspring of nonconsanguineous and consanguineous couples. We included, in all analyzed situations, the following factors: (a) a generic degree of parental consanguinity; (b) a variable proportion of environmental (non-genetic) cases of the defect, so that the formulae can be easily applied to populations with any epidemiological profile; (c) a variable number of normal sibs of the propositus. Besides presenting the logic and the detailed derivation of all original formulae, we present tables for immediate use, with the numerical values of the recurrence risks as a function of the variables...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nonsyndromic deafness; Recurrence risks; Genetic counseling.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200004

Cloning of the Bacillus thuringiensis serovar sotto chitinase (Schi) gene and characterization of its protein
Provedor de dados: Genetics and Molecular Biology
Autores: Zhong,Wan-Fang; Fang,Ji-Chao; Cai,Ping-Zhong; Yan,Wen-Zhao; Wu,Jie; Guo,Hui-Fang.
Chitinase plays a positive role in the pathogenicity of Bacillus thuringiensis to insect pests. We used touchdown PCR to clone the chitinase (Schi) gene from Bacillus thuringiensis serovar sotto (Bt sotto) chromosomal DNA. Our DNA sequencing analysis revealed that the Bt sotto Schi gene consists of an open reading frame (ORF) of 2067 nucleotides with codes for the chitinase precursor. We also found that the putative promoter consensus sequences (the -35 and -10 regions) of the Bt soto Schi gene are identical to those of the chiA71 gene from Bt Pakistani, the chiA74 gene from Bt kenyae and the ichi gene from Bt israelensis. The Schi chitinase precursor is 688 amino acids long with an estimated molecular mass of 75.75 kDa and a theoretical isoelectric point...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus thuringiensis serovar sotto; Chitinase; Touchdown PCR; Gene cloning; Characterization analysis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500026

Interspecific hybridization and inbreeding effect in seed from a Eucalyptus grandis x E. urophylla clonal orchard in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Campinhos,Eduardo N.; Peters-Robinson,Ingrid; Bertolucci,Fernando L.; Alfenas,Acelino C.
We used allozyme markers to estimate the amount of natural hybridization between Eucalyptus grandis and E. urophylla in a 7.4-hectare commercial hybrid-seed orchard planted in Espírito Santo, Brazil. This orchard was planted in 1982 using a honeycomb design, with each hexagonal plot containing one E. grandis tree surrounded by six E. urophylla trees. There were 267 replicated hexagonal plots in the orchard. Seeds were harvested from the E. grandis clone only. The multilocus outcrossing rate estimated for the E. grandis clone averaged 70.2%, ranging from 33.0 to 99.0% among individual trees. Contaminant pollination, inferred from progeny genotypes containing alleles not present in the seven parental clones, accounted for 14.4% of the hybrid seed....
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300014

Distribution and conservation of the transposable element gypsy in drosophilid species
Provedor de dados: Genetics and Molecular Biology
Autores: Herédia,Fabiana; Loreto,Elgion Lúcio S.; Valente,Vera Lúcia S.
In an attempt to understand the dynamics of transposable elements (T'S) in the genome of host species, we investigated the distribution, representativeness and conservation of DNA sequences homologous to the Drosophila melanogaster gypsy retrotransposon in 42 drosophilid species. Our results extended the knowledge about the wide distribution of gypsy in the genus Drosophila, including several Neotropical species not previously studied. The gypsy-like sequences showed high divergence compared to the D. melanogaster gypsy element. Furthermore, the conservation of the restriction sites between gypsy sequences from phylogenetically unrelated species pointed to a more complex evolutionary picture, which includes the possibility of the horizontal transfer events...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila; Retrotransposon; Gypsy; Southern blot; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100023

Association of a locus on rat chromosome 4 with anxiety-related behaviors in two selectively bred rat lines
Provedor de dados: Genetics and Molecular Biology
Autores: Hameister,Thaïs M.; Izídio,Geison S.; Valiati,Victor H.; Ramos,André.
The Floripa H and L rat lines, selected for high and low locomotion in the central aversive area of an open field, a widely used emotionality test, were proposed as a model for studying the genetic basis of anxiety. The present study aimed to verify if the QTL Ofil1, mapped to rat chromosome 4 and previously identified as being related to emotionality in another population of rats, contributes to the behavioral variability observed in the Floripa rat lines. To this purpose, rats of five generations of selective breeding were genotyped for two polymorphic markers, D4RAT59 and D4MGH27, flanking Ofil1. Changes in genotype and allele frequencies throughout generations were evaluated in both H and L lines, in order to assess if the bidirectional selection based...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: QTL; Emotionality; Rat lines; Behavior genetics; Open field.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500008

Characterization of the complete mitochondrial genome and a set of polymorphic microsatellite markers through next-generation sequencing for the brown brocket deer Mazama gouazoubira
Provedor de dados: Genetics and Molecular Biology
Autores: Caparroz,Renato; Mantellatto,Aline M.B.; Bertioli,David J.; Figueiredo,Marina G.; Duarte,José Maurício B.
The complete mitochondrial genome of the brown brocket deer Mazama gouazoubira and a set of polymorphic microsatellite markers were identified by 454-pyrosequencing. De novo genome assembly recovered 98% of the mitochondrial genome with a mean coverage of 9-fold. The mitogenome consisted of 16,356 base pairs that included 13 protein-coding genes, two ribosomal subunit genes, 22 transfer RNAs and the control region, as found in other deer. The genetic divergence between the mitogenome described here and a previously published report was ∼0.5%, with the control region and ND5 gene showing the highest intraspecific variation. Seven polymorphic loci were characterized using 15 unrelated individuals; there was moderate genetic variation across most loci (mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Microsatellites; Mitogenome; Population genetics; 454-pyrosequencing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300338

The complete mitochondrial genome sequence of the black-capped capuchin (Cebus apella)
Provedor de dados: Genetics and Molecular Biology
Autores: Bi,Xiao-xin; Huang,Ling; Jing,Mei-dong; Zhang,Li; Feng,Pei-yong; Wang,Ai-yun.
The phylogenetic relationships of primates have been extensively investigated, but key issues remain unresolved. Complete mitochondrial genome (mitogenome) data have many advantages in phylogenetic analyses, but such data are available for only 46 primate species. In this work, we determined the complete mitogenome sequence of the black-capped capuchin (Cebus apella). The genome was 16,538 bp in size and consisted of 13 protein-coding genes, 22 tRNAs, two rRNAs and a control region. The genome organization, nucleotide composition and codon usage did not differ significantly from those of other primates. The control region contained several distinct repeat motifs, including a putative termination-associated sequence (TAS) and several conserved sequence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cebus apella; Control region; Mitochondrial genome; Phylogenomics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300023

PCR-mediated recombination in development of microsatellite markers: mechanism and implications
Provedor de dados: Genetics and Molecular Biology
Autores: Roratto,Paula A.; Buchmann,Darine; Santos,Sandro; Bartholomei-Santos,Marlise L.
Protocols for microsatellite-enrichment libraries have been widely applied to several species in order to supply the most informative molecular markers for population and inbreeding studies. One drawback of these protocols is the ratio of designed primer pairs that fail to amplify the expected fragment, even after exhaustive optimization attempts. A possible cause of unsuccessful microsatellite primers may be that such loci are artifacts resulting from chimeric PCR products, instead of real genomic sequences. The microsatellite-enriched library constructed for Aegla longirostri (Crustacea, Decapoda, Anomura) showed that 29% of sequenced clones were chimeric products because these sequences shared one of the flanking regions around the same repeat motif but...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chimeric PCR product; Microsatellite isolation; Recombination; Simple sequence repeats (SSR).
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100012

Esterase-3 polymorphism in the sugarcane borer Diatraea saccharalis (Lepidoptera, Pyralidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Ruvolo-Takasusuki,Maria Claudia C.; Machado,Maria de Fátima P.S.; Conte,Hélio.
The migration rate of esterases and their substrate specificity for 4-methylumbelliferyl esters (acetate, propionate, and butyrate) and alpha- and beta-naphthyl esters were analyzed in Diatraea saccharalis by starch gel electrophoresis. Substrate preference of esterases was observed with Est-2 and Est-8 isozymes showing substrate specificity for 4-methylumbelliferyl esters and Est-4 isozyme showing specificity for 4-methylumbelliferyl butyrate and alpha-naphthyl butyrate. Allele variation was detected at the Est-3 locus. Two alleles, Est-3F and Est-3S, were identified in pupae with fluorogenic and ester-naphthyl substrates. Chi-square analysis showed no differences between the observed genotypic frequencies and those expected on the basis of Hardy-Weinberg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esterase; Genetic variability; Isozymes; Moth; Sugarcane borer.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100012

Prevalence of hereditary risk factors for thrombophilia in Belém, Brazilian Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Yoshioka,France Keiko Nascimento; Araújo,Amélia Góes; Tavella,Marli Haydee; Hamoy,Igor Guerreiro; Guerreiro,João Farias.
Different risk factors for venous thromboembolism (VTE) have been identified, including hereditary abnormalities in the mechanisms of coagulation and fibrinolysis. We investigated five genetic polymorphisms (FVL G1691A, FII G20210A, MTHFR C677T, TAFI A152G and TAFI T1053C) associated with VTE in individuals from the city of Belém in the Brazilian Amazon who had no history of VTE. No significant difference was found between the observed and expected genotype frequencies for the loci analyzed. We found high frequencies of MTHFR C677T (33.9%) and TAFI T1053C (74%) and low frequencies of FVL (1.6%), FII G20210A (0.8%) and TAFI A152G (0.8%). The FVL G1691A, FII G20210A and MTHFR C677T frequencies were similar to those for European populations and populations of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Factor V Leiden; Prothrombin; MTHFR; TAFI; Thrombophilia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100007

A phylogenetic analysis of Brycon and Henochilus (Characiformes, Characidae, Bryconinae) based on the mitochondrial gene 16S rRNA
Provedor de dados: Genetics and Molecular Biology
Autores: Hilsdorf,Silva; Oliveira,Claudio; Lima,Flávio César Thadeo de; Matsumoto,Cristianne Kayoko.
The genus Brycon, the largest subunit of the Bryconinae, has 42 valid species distributed from southern Mexico to the La Plata River in Argentina. Henochilus is a monotypic genus, comprising a single species (H. wheatlandii) found in the upper Rio Doce basin. In the present study, partial sequences of the mitochondrial gene 16S were obtained for fifteen species of Brycon and for Henochilus wheatlandii. The results showed that the genus Brycon is paraphyletic, since Henochilus is the sister-group of B. ferox and B. insignis. The most basal species analyzed were the trans-Andean species B. henni, B. petrosus, and B. chagrensis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogeny; Molecular systematics; Mitochondrial DNA; Fish evolution; Systematic.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200034

Cyclic pamidronate treatment for osteogenesis imperfecta: Report from a Brazilian reference center
Provedor de dados: Genetics and Molecular Biology
Autores: Pinheiro,Bruna; Zambrano,Marina B.; Vanz,Ana Paula; Brizola,Evelise; Souza,Liliane Todeschini de; Félix,Têmis Maria.
Abstract Treatment of moderate and severe forms of osteogenesis imperfecta (OI) with cyclic pamidronate at the Reference Center for OI Treatment in Southern Brazil was studied. A retrospective cohort study was conducted from 2002 to 2012. Data were obtained during inpatient (drug infusion) and outpatient care. Clinical data, including the presence of blue sclerae, dentinogenesis imperfecta, history and site of the fractures, biochemical data, including calcium, phosphorus, and alkaline phosphatase levels, were systematically collected. Bone mineral density (BMD) was measured using dual energy X-ray absorptiometry (DXA). Forty-five patients (26 females) were included in the study, and the age of the patients at the time of diagnosis ranged from 1 to 144...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteogenesis imperfecta; Bone fracture; Clinical features; Pamidronate treatment; Compliance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200252

DNA Segredos &amp;amp; Mistérios
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Sérgio Olavo Pinto da.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400028

Identification of citrus expressed sequence tags (ESTs) encoding pleiotropic drug resistance (PDR)-like proteins
Provedor de dados: Genetics and Molecular Biology
Autores: Amaral,Alexandre Morais do; Saito,Daniel; Formighieri,Eduardo Fernandes; Rabello,Edenilson; Souza,Adriane N. de; Silva-Stenico,Maria Estela; Tsai,Siu Mui.
Pleiotropic drug resistance (PDR) proteins, a subfamily of the ATP-binding cassette (ABC) transporters, have been recently shown to play a role in plant defense against biotic and abiotic stresses. However, nothing is known about their expression in citrus. To investigate the occurrence of PDR homologues in citrus species, we have surveyed EST sequences from different tissues and conditions of the Citrus Expressed Sequence Tags (CitEST) database, through sequence similarity search analyses and inspections for characteristic PDR domains. Multiple sequence alignments, prediction of transmembrane topology and phylogenetic analysis of PDR-like proteins were additionally performed. This study allowed the identification of nine putative proteins showing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ABC transporters; CitEST; Expressed sequence tag; Transcriptome; Stress.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500014

Assessment of the genotoxic impact of pesticides on farming communities in the countryside of Santa Catarina State, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Salvagni,Jaqueli; Ternus,Raquel Zeni; Fuentefria,Alexandre Meneghello.
The aim of this study was to assess the use of pesticides on farms located in the Lambedor River watershed in Guatambu, State of Santa Catarina, as well as to determine, by micronucleus testing, the risk of genotoxic impact. Samples from locally collected Cyprinus carpio, Hypostomus punctatus, Rhamdia quelen and Oreochromis niloticus gave evidence of a mean increase in micronuclei frequency from 6.21 to 13.78 in 1,000 erythrocytes, a clear indication of the genotoxic potenciality of pesticide residues in regional dams, and their significant contribution to local environmental contamination.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pesticides; Environment; Micronucleus test.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100021

A novel de novo COL1A1 mutation in a Thai boy with osteogenesis imperfecta born to consanguineous parents
Provedor de dados: Genetics and Molecular Biology
Autores: Tongkobpetch,Siraprapa; Limpaphayom,Noppachart; Sangsin,Apiruk; Porntaveetus,Thantrira; Suphapeetiporn,Kanya; Shotelersuk,Vorasuk.
Abstract Osteogenesis imperfecta (OI) is genetically heterogeneous. Mutations in COL1A1 and COL1A2 are responsible for at least 90% of the cases, which are transmitted in an autosomal dominant manner or are de novo events. We identified a Thai boy with OI whose parents were first cousins. Because the proband was the product of a consanguineous marriage, we hypothesized that he might be homozygous for a mutation in a known gene causing a recessive form of OI. Using whole exome sequencing (WES), we did not find any pathogenic mutations in any known gene responsible for an autosomal recessive form of OI. Instead, we identified a COL1A1 frameshift mutation, c.1290delG (p.Gly431Valfs*110) in heterozygosis. By Sanger sequencing, the mutation was confirmed in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteogenesis imperfect; COL1A1; Exome sequencing; Next generation sequencing; Thai.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500763

Changes in the structure and pigmentation of the eyes of honeybee (Apis mellifera L.) queens with the "limão" mutation
Provedor de dados: Genetics and Molecular Biology
Autores: Chaud-Netto,José; Cruz-Landim,Carminda da.
This study describes the ultrastructural differences between the compound eyes of ch li/ch li and Ch/ch li honeybee queens. Heterozygous "limão" bees had an almost normal ultrastructural organization of the ommatidia, but there were some alterations, including small vacuoles in the crystalline cones and a loss of pigment by primary pigmentary cells. In homozygous bees many ommatidia had very deformed crystalline cones and there were some bipartite rhabdoma. There was a reduction in the amount of pigment in the primary and secondary pigmentary cells and receptor cells (retinulae) of mutant eyes. However, the eyes of both heterozygous and homozygous queens had the same type of pigment granules. Certain membrane-limited structures containing pigment granules...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100017

Autosomal rearrangement in Gryllus assimilis Fabricius, 1775 (Orthoptera, Gryllidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Zefa,Edison.
Gryllus assimilis L. has a karyotype of 2n = 29 (X0, male) and 30 (XX, female). The above karyotype was encountered along with another in which 2n = 28 (X0, male) and 2n = 29 (XX, female) in a population from the outskirts of Rio Claro city (São Paulo State, Brazil). Of eight specimens studied, five had the heterozygous karyotype involving a translocation and three had the basic karyotype. There were no individuals homozygous for the rearrangement. The heterozygous karyotype was the result of a chromosomal rearrangement between chromosome pairs 6 and 10, both of which were initially submetacentric. The members of the sixth pair normally have two constrictions in the small arm, with a satellite at the chromosome tip. The chromosome of the tenth pair...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300008

Origin, evolution and genome distribution of microsatellites
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Eder Jorge; Pádua,Juliano Gomes; Zucchi,Maria Imaculada; Vencovsky,Roland; Vieira,Maria Lúcia Carneiro.
Microsatellites, or simple sequence repeats (SSRs), have been the most widely applied class of molecular markers used in genetic studies, with applications in many fields of genetics including genetic conservation, population genetics, molecular breeding, and paternity testing. This range of applications is due to the fact that microsatellite markers are co-dominant and multi-allelic, are highly reproducible, have high-resolution and are based on the polymerase chain reaction (PCR). When first introduced, the development of microsatellite markers was expensive but now new and efficient methods of repetitive sequence isolation have been reported, which have led to reduced costs and microsatellite-technology has been increasingly applied to several species,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Molecular genetics; Genetic structure of populations.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200018

A rapid detection method for PAI-1 promoter insertion/deletion polymorphism (4G/5G)
Provedor de dados: Genetics and Molecular Biology
Autores: Annichino-Bizzacchi,Joyce M.; Pugliese,Luciana; Arruda,Valder R.
Plasminogen activator inhibitor-1 (PAI-1) is an important inhibitor of fibrinolysis, and increased levels of PAI-1 are associated with atheroma and myocardial infarction. A common 4G/5G insertion/deletion polymorphism located in the promoter region of PAI-1 gene has been described associated with PAI-1 activity in plasma levels. Genotyping of this polymorphism is commonly conducted with an allele-specific oligonucleotide melting technique. In the present study, we describe a quick, easy method for genotyping 4G/5G polymorphism in the promoter region of the PAI-1 gene.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300004

Identification of RAPD and SCAR markers associated with yield traits in the Indian tropical tasar silkworm Antheraea mylitta drury
Provedor de dados: Genetics and Molecular Biology
Autores: Dutta,Suhrid R.; Kar,Prasanta K.; Srivastava,Ashok K.; Sinha,Manoj K.; Shankar,Jai; Ghosh,Ananta K.
The tropical tasar silkworm, Antheraea mylitta, is a semi-domesticated vanya silk-producing insect of high economic importance. To date, no molecular marker associated with cocoon and shell weights has been identified in this species. In this report, we identified a randomly amplified polymorphic DNA (RAPD) marker and examined its inheritance, and also developed a stable diagnostic sequence-characterized amplified region (SCAR) marker. Silkworms were divided into groups with high (HCSW) and low (LCSW) cocoon and shell weights, and the F2 progeny of a cross between these two groups were obtained. DNA from these silkworms was screened by PCR using 34 random primers and the resulting RAPD fragments were used for cluster analysis and discriminant function...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antheraea mylitta; Quantitative trait; RAPD; SCAR marker; Tasar silkworm.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500007

New evidence for balancing selection at the HLA-G locus in South Amerindians
Provedor de dados: Genetics and Molecular Biology
Autores: Veit,Tiago Degani; Cazarolli,Juciana; Salzano,Francisco Mauro; Schiengold,Marion; Chies,José Artur Bogo.
HLA-G is a non-classical HLA (Human Leukocyte Antigen) molecule characterized by limited tissue distribution under normal physiological conditions and low variability at both DNA and protein levels. Several studies suggest that HLA-G could play a role, as an immunoregulatory molecule, in situations as diverse as transplantation, cancer, viral infections and inflammatory diseases. A total of 237 individuals from 21 South American tribes speaking nine different linguistic families were studied in relation to the 14 bp insertion/deletion polymorphism at the HLA-G gene. A consistent (seven in nine) excess of heterozygosity in samples classified by language was obtained. Our data supply evidences for balancing selection acting at the HLA-G 14 bp INDEL region....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA-G 14 bp INDEL; Balancing selection; Amerindians; Pathogen resistance.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600005

Ionizing radiation-induced DNA injury and damage detection in patients with breast cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Borrego-Soto,Gissela; Ortiz-López,Rocío; Rojas-Martínez,Augusto.
Abstract Breast cancer is the most common malignancy in women. Radiotherapy is frequently used in patients with breast cancer, but some patients may be more susceptible to ionizing radiation, and increased exposure to radiation sources may be associated to radiation adverse events. This susceptibility may be related to deficiencies in DNA repair mechanisms that are activated after cell-radiation, which causes DNA damage, particularly DNA double strand breaks. Some of these genetic susceptibilities in DNA-repair mechanisms are implicated in the etiology of hereditary breast/ovarian cancer (pathologic mutations in the BRCA 1 and 2 genes), but other less penetrant variants in genes involved in sporadic breast cancer have been described. These same genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Ionizing radiation; DNA damage; DNA double strand break; DNA repair analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400420

Metaphase I orientation of Robertsonian trivalents in the water-hyacinth grasshopper, Cornops aquaticum (Acrididae, Orthoptera)
Provedor de dados: Genetics and Molecular Biology
Autores: Colombo,Pablo César.
Trivalents resulting from polymorphic Robertsonian rearrangements must have a regular orientation in metaphase I if the polymorphisms are to be maintained. It has been argued that redistribution of proximal and interstitial chiasmata to more distal positions is necessary for a convergent orientation, the only one that produces viable gametes. Cornops aquaticum is a South-American grasshopper that lives and feeds on water-hyacinths, and has three polymorphic Robertsonian rearrangements in its southernmost distribution area in Central Argentina and Uruguay. The orientation of trivalents in metaphase I, the formation of abnormal spermatids and the frequency and position of chiasmata in the trivalents, was analysed in a polymorphic population of C. aquaticus....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cornops aquaticum; Robertsonian rearrangements; Trivalents; Metaphase I orientation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100013

Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
Provedor de dados: Genetics and Molecular Biology
Autores: Pinheiro,Fábio; Palma-Silva,Clarisse; Barros,Fábio de; Cozzolino,Salvatore.
In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidendrum; Orchidaceae; Short tandem repeat; Cross-amplification.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021

Detection of Metarhizium anisopliae var. anisopliae within infected sugarcane borer Diatraea saccharalis (Lepidoptera, Pyralidae) using specific primers
Provedor de dados: Genetics and Molecular Biology
Autores: Destéfano,Ricardo Henri Rodrigues; Destéfano,Suzete A. Lanza; Messias,Claudio Luiz.
In order to construct specific primers for the detection and identification of the entomopathogenic fungus Metarhizium within infected sugarcane borer (Diatraea saccharalis) larvae we analyzed the ITS1 -5.8S- ITS2 rDNA regions of strains and varieties of M. anisopliae, M. album and M. flavoviride. The PCR amplification of these regions yielded a unique fragment of approximately 540 bp for M. anisopliae variety anisopliae strains E9, B/Vi and C (isolated in Brazil), 600 pb for M. a. anisopliae strain 14 (isolated in Australia), 650 bp for the M. album and 600 bp for M. flavoviride strains. The PCR products were digested with different restriction endonucleases (Afa I, Alu I, Dde I, Hae III, Hpa II and Sau 3A) and the PCR-RFLP profiles showed clear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metarhizium anisopliae; Entomopathogenic fungi; PCR-RFLP; ITS region.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200020

Assessment of the myostatin Q204X allele using an allelic discrimination assay
Provedor de dados: Genetics and Molecular Biology
Autores: Sifuentes-Rincón,Ana M.; Puentes-Montiel,Herlinda E.; Moreno-Medina,Víctor R.; Rosa-Reyna,Xóchitl F. de la.
An allelic discrimination assay was designed and used to determine the genotypic and allelic frequencies of the myostatin (MSTN) gene Q204X allele from two Mexican Full-French herds. The assay is a simple high throughput genotyping method that could be applied to investigate the effect of the Q204X allele on the Charolais breed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Charolais; Carcass marker; Allelic form; SNP detection.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300017

Replicating animal mitochondrial DNA
Provedor de dados: Genetics and Molecular Biology
Autores: McKinney,Emily A.; Oliveira,Marcos T.
The field of mitochondrial DNA (mtDNA) replication has been experiencing incredible progress in recent years, and yet little is certain about the mechanism(s) used by animal cells to replicate this plasmid-like genome. The long-standing strand-displacement model of mammalian mtDNA replication (for which single-stranded DNA intermediates are a hallmark) has been intensively challenged by a new set of data, which suggests that replication proceeds via coupled leading-and lagging-strand synthesis (resembling bacterial genome replication) and/or via long stretches of RNA intermediates laid on the mtDNA lagging-strand (the so called RITOLS). The set of proteins required for mtDNA replication is small and includes the catalytic and accessory subunits of DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA replication; Mitochondria; MtSSB; Pol γ; Twinkle.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300002

A role for adeno-associated viral vectors in gene therapy
Provedor de dados: Genetics and Molecular Biology
Autores: Coura,Renata dos Santos; Nardi,Nance Beyer.
Gene therapy constitutes a therapeutic intervention based on modification of the genetic material of living cells, by correcting genetic defects or overexpressing therapeutic proteins. The success of gene therapy protocols depends on the availability of therapeutically suitable genes, appropriate gene delivery systems and proof of safety and efficacy. Recent advances on the development of gene delivery systems, particularly on viral vectors engineering and improved gene regulatory systems, have led to marked progress in this field. Although the available vector systems can successfully transfer genes into cells, the ideal delivery vehicle has not been found. In this context, adeno-associated virus vectors (AAV) are arising as a promising tool for a wide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adeno-associated virus; AAV-based recombinant vectors; Gene therapy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100001

Clinical, genetical, radiological, and anatomopathological survey of 17 patients with lethal osteochondrodysplasias
Provedor de dados: Genetics and Molecular Biology
Autores: Galera,Marcial Francis; Patrício,Francy Reis da S.; Cernach,Mirlene Cecília S. Pinho; Lederman,Henrique Manoel; Brunoni,Decio.
Seventeen patients thought to have lethal osteochondrodysplasias were evaluated. Diagnosis was established through clinical evaluation, radiological studies and necropsy. Genetic counseling was provided to the affected patient's families. Specific diagnosis was confirmed in 16 cases. Nosologic diagnosis was done through clinical evaluation. However, the most efficient method for verifying the diagnosis was a skeletal radiological study. This fact corroborates the orientation of the International Classification of Osteochondrodysplasias (International Working Group on Constitutional Disease of Bone, 1992) in which a radiological criterion was adopted as the most relevant for classification of osteochondrodysplasias. An anatomopathological study was also...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200017

Crodowaldo Pavan (1919-2009): one of a kind
Provedor de dados: Genetics and Molecular Biology
Autores: Perondin,André L.P.; Morgante,João S.
Tipo: Info:eu-repo/semantics/report
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300001

Association between cervical lesion grade and micronucleus frequency in the Papanicolaou test
Provedor de dados: Genetics and Molecular Biology
Autores: Bueno,Caroline Tanski; Silva,Cláudia Maria Dornelles da; Barcellos,Regina Bones; Silva,Juliana da; Santos,Carla Rossana dos; Menezes,João Evangelista Sampaio; Menezes,Honório Sampaio; Rossetti,Maria Lucia Rosa.
The aim of this study was to evaluate the association between the frequency of micronuclei (MN) and the cellular changes detected in the conventional Papanicolaou test. One hundred and seventy-four Papanicolaou test smears with cellular changes were examined. MN screening was done in cytopathological smears by counting 1,000 cervical cells in a light microscope. MN frequencies were significantly higher in the group with cellular changes compared to the control group (p &lt; 0.001). The mean MN frequencies were 0.95 ± 1.12 (mean ± SD) in the control group (n = 223), 2.98 ± 1.20 in individuals with atypical squamous cells of undetermined significance (ASC-US) (n = 50), 4.04 ± 1.45 in cervical intraepithelial neoplasia (CIN) I (n = 52), 5.97 ± 1.83 in CIN II...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical cancer; Cervical lesions; Micronucleus; Papanicolaou test.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400004

Cytotaxonomy of some species and of interspecific hybrids of Pennisetum (Poaceae, Poales)
Provedor de dados: Genetics and Molecular Biology
Autores: Techio,Vânia Helena; Davide,Lisete Chamma; Pereira,Antônio Vander; Bearzoti,Eduardo.
Cytotaxonomic studies were carried out on 26 accessions of Pennisetum spp. originating from the Active Germplasm Bank of Embrapa Gado de Leite-Juiz de Fora, Minas Gerais, Brazil.This study presents cytogenetics and reproductive morphological data for each one of these accessions, which allowed groupings and suggest botanical identifications to be established. The metaphases of the accessions characterized as P. purpureum confirmed the presence of 2n = 28 chromosomes that have already been described for this species. For the hybrids between P. purpureum and P. glaucum and for the hexaploids, resulting from the chromosome duplication of these hybrids, 2n = 21 and 2n = 42 chromosomes were confirmed, respectively. The wild accession identified as P. setosum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pennisetum; Cytogenetic; Taxonomy; Germplasm; Genetic divergence.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200014

Genetic parameters and relationships between growth traits and scrotal circumference measured at different ages in Nellore cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Boligon,Arione Augusti; Baldi,Fernando; Albuquerque,Lucia Galvão de.
Records from 106,212 Nellore animals, born between 1998 and 2006, were used to estimate (co)variance components and genetic parameters for birth weight (BW), average weight gains from birth to weaning (GBW), average weight gains from weaning to after yearling (GWAY), weaning hip height (WHH), postweaning hip height (PHH) and scrotal circumferences at 9 (SC9), 12 (SC12) and 15 (SC15) months of age. (Co)variance components were estimated by an animal model using multi-trait analysis. Heritability estimates for BW, GBW, GWAY, WHH, PHH, SC9, SC12 and SC15 were 0.31 ± 0.01; 0.25 ± 0.02; 0.30 ± 0.04; 0.51 ± 0.04; 0.54 ± 0.04; 0.39 ± 0.01; 0.41 ± 0.01 and 0.44 ± 0.02, respectively. Genetic correlations between growth traits ranged from 0.09 ± 0.01 to 0.88 ± 0.01,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Genetic correlation; Hip height; Average weight gain.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200010

Lack of evidence for intermolecular epistatic interactions between adiponectin and resistin gene polymorphisms in Malaysian male subjects
Provedor de dados: Genetics and Molecular Biology
Autores: Lau,Cia-Hin; Muniandy,Sekaran.
Epistasis (gene-gene interaction) is a ubiquitous component of the genetic architecture of complex traits such as susceptibility to common human diseases. Given the strong negative correlation between circulating adiponectin and resistin levels, the potential intermolecular epistatic interactions between ADIPOQ (SNP+45T &gt; G, SNP+276G &gt; T, SNP+639T &gt; C and SNP+1212A &gt; G) and RETN (SNP-420C &gt; G and SNP+299G &gt; A) gene polymorphisms in the genetic risk underlying type 2 diabetes (T2DM) and metabolic syndrome (MS) were assessed. The potential mutual influence of the ADIPOQ and RETN genes on their adipokine levels was also examined. The rare homozygous genotype (risk alleles) of SNP-420C &gt; G at the RETN locus tended to be co-inherited...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipokine; ADIPOQ; Epistasis; Interaction; RETN.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100005

Cytogenetics and cytotaxonomy of some Brazilian species of Cymbidioid orchids
Provedor de dados: Genetics and Molecular Biology
Autores: Félix,Leonardo Pessoa; Guerra,Marcelo.
The Cymbidioid phylad presents the widest chromosome number variation among orchids, with records varying from 2n = 10 in Psygmorchis pusilla to 2n = 168 in two species of Oncidium. In the present work, a total of 44 species were studied belonging to 20 Cymbidioid genera, as a contribution to clarifying the karyological evolution of the group. All the plants investigated were collected in Brazil, mainly in the northeast region. The chromosome variation found was similar to that previously registered in the literature. Chromosome numbers observed were: 2n = 54 (subtribe Eulophiinae), 2n = 44, 46, 92 (subtribe Cyrtopodiinae), 2n = 54, ca. 108 (subtribe Catasetinae), 2n = 52, ca. 96 (subtribe Zygopetalinae), 2n = 40, 80 (subtribe Lycastinae), 2n = 40, 42...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400041

Purine and pyrimidine nucleotide metabolism in Mollicutes
Provedor de dados: Genetics and Molecular Biology
Autores: Bizarro,Cristiano Valim; Schuck,Desirée Cigaran.
Several mollicute genome projects are underway, offering unique opportunities to study genes and metabolic pathways on a genome-wide scale. Here, we have analyzed the conservation and diversity of purine and pyrimidine metabolism in mycoplasmas. An evaluation of discrepancies between genomic analysis and enzymatic data revealed interesting aspects about these organisms. We found important examples in which enzyme activity was reported without the annotation of a corresponding gene. An interesting example concerns phosphopentomutase. In Mollicutes, we have identified CDSs orthologous to sequences recently identified as new phosphopentomutases in archaeobacteria that are structurally related to phosphomannomutases. It is suggested that these sequences could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mollicutes; Purine; Pyrimidine; Metabolism; Metabolic pathways.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200005

Cytogenetic variation of repetitive DNA elements in Hoplias malabaricus (Characiformes - Erythrinidae) from white, black and clear water rivers of the Amazon basin
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Fabíola Araújo dos; Marques,Diego Ferreira; Terencio,Maria Leandra; Feldberg,Eliana; Rodrigues,Luís Reginaldo R.
Abstract Hoplias malabaricus is a common fish species occurring in white, black and clear water rivers of the Amazon basin. Its large distribution across distinct aquatic environments can pose stressful conditions for dispersal and creates possibilities for the emergence of local adaptive profiles. We investigated the chromosomal localization of repetitive DNA markers (constitutive heterochromatin, rDNA and the transposable element REX-3) in populations from the Amazonas river (white water), the Negro river (black water) and the Tapajós river (clear water), in order to address the variation/association of cytogenomic features and environmental conditions. We found a conserved karyotypic macrostructure with a diploid number of 40 chromosomes (20...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenomics; FISH; RDNA; REX 3; Tapajós river.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100040

In silico characterization and expression analyses of sugarcane putative sucrose non-fermenting-1 (SNF1) related kinases
Provedor de dados: Genetics and Molecular Biology
Autores: Carraro,Dirce Maria; Lambais,Marcio R.; Carrer,Helaine.
Sucrose non-fermenting-1-related protein kinases (SnRKs) may play a major role in regulating gene expression in plant cells. This family of regulatory proteins is represented by sucrose non-fermenting-1 (SNF1) protein kinase in Saccharomyces cerevisiae, AMP-activated protein kinases (AMPKs) in mammals and SnRKs in higher plants. The SnRK family has been reorganized into three subfamilies according to the evolutionary relationships of their amino acid sequences. Members of the SnRK subfamily have been identified in several plants. There is evidence that they are involved in the nutritional and/or environmental stress response, although their roles are not yet well understood. We have identified at least 22 sugarcane expressed sequence tag (EST) contigs...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100006

Teaching evolutionary biology
Provedor de dados: Genetics and Molecular Biology
Autores: Tidon,Rosana; Lewontin,Richard C.
Evolutionary Biology integrates several disciplines of Biology in a complex and interactive manner, where a deep understanding of the subject demands knowledge in diverse areas. Since this knowledge is often inaccessible to the majority of specialized professionals, including the teachers, we present some reflections in order to stimulate discussions aimed at the improvement of the conditions of education in this area. We examine the profile of evolutionary teaching in Brazil, based on questionnaires distributed to teachers in Secondary Education in the Federal District, on data provided by the "National Institute for Educational Studies and Research", and on information collected from teachers working in various regions of this country. Issues related to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Education; Evolutionary biology; Misconception; Teaching.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100021

Novel mutations in the TBX5 gene in patients with Holt-Oram Syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Porto,Marianna P.R.; Vergani,Naja; Carvalho,Antonio Carlos C.; Cernach,Mirlene C.S.P.; Brunoni,Decio; Perez,Ana Beatriz A.
The Holt-Oram syndrome (HOS) is an autosomal dominant condition characterized by upper limb and cardiac malformations. Mutations in the TBX5 gene cause HOS and have also been associated with isolated heart and arm defects. Interactions between the TBX5, GATA4 and NKX2.5 proteins have been reported in humans. We screened the TBX5, GATA4, and NKX2.5 genes for mutations, by direct sequencing, in 32 unrelated patients presenting classical (8) or atypical HOS (1), isolated congenital heart defects (16) or isolated upper-limb malformations (7). Pathogenic mutations in the TBX5 gene were found in four HOS patients, including two new mutations (c.374delG; c.678G &gt; T) in typical patients, and the hotspot mutation c.835C &gt; T in two patients, one of them with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Holt-Oram syndrome; Congenital heart disease; TBX5 gene; GATA gene; NKX2.5 gene; Mutation analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200006

Fine-scale spatial genetic structure of Dalbergia nigra (Fabaceae), a threatened and endemic tree of the Brazilian Atlantic Forest
Provedor de dados: Genetics and Molecular Biology
Autores: Buzatti,Renata Santiago de Oliveira; Ribeiro,Renata Acácio; Lemos Filho,José Pires de; Lovato,Maria Bernadete.
The Atlantic Forest is one of the most diverse ecosystems in the world and considered a hotspot of biodiversity conservation. Dalbergia nigra (Fabaceae) is a tree endemic to the Brazilian Atlantic Forest, and has become threatened due to overexploitation of its valuable timber. In the present study, we analyzed the genetic diversity and fine-scale spatial genetic structure of D. nigra in an area of primary forest of a large reserve. All adult individuals (N = 112) were sampled in a 9.3 ha plot, and genotyped for microsatellite loci. Our results indicated high diversity with a mean of 8.6 alleles per locus, and expected heterozygosity equal to 0.74. The co-ancestry coefficients were significant for distances among trees up to 80 m. The Sp value was equal to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dalbergia nigra; Atlantic Forest; Conservation genetics; Fine-scale spatial genetic structure; Sp statistic.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500019

Genetic diversity of Xanthomonas axonopodis pv. citri based on plasmid profile and pulsed field gel electrophoresis
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Flávia Maria de Souza; Caramori,Lázara Pereira Campos; Leite Júnior,Rui Pereira.
Xanthomonas axonopodis pv. citri strains that cause disease in citrus were investigated by pulsed field and plasmid profile analysis. For the first method, genomic DNA was digested by the rare-cutting enzymes Xba I and Vsp I. The strains evaluated were collected in seven different States of Brazil and in Argentina, Bolivia, Paraguay and Uruguay. Genetic variability was found among strains of X. axonopodis pv. citri from different geographical areas Argentina, Bolivia and Uruguay, with similarities varying from 0.62 to 0.83. However, the strains collected in Brazil, despite being from different States, have shown a genetic similarity ranging from 0.83 to 1.00. Cluster analysis showed a relationship between genomic similarity and geographical origin of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Genetic variability; Bacterium; Citrus; PFGE.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300020

Metaphase karyotypes of Anopheles (Nyssorhynchus) darlingi Root and A. (N.) nuneztovari Gabaldón (Diptera; Culicidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Rafael,Míriam Silva; Tadei,Wanderli Pedro.
Metaphase karyotypes of Anopheles (Nyssorhynchus) darlingi and Anopheles (N.) nuneztovari from Manaus and Highway BR-174 (Manaus-Boa Vista), State of Amazonas, and Macapá, State of Amapá, Brazil, showed 2n = 6. They consisted of a pair of metacentric (chromosome II) and a pair of submetacentric (chromosome III) autosomes as well as sex chromosomes X and Y. In the sex chromosomes, the X was acrocentric in A. darlingi and submetacentric in A. nuneztovari. The Y chromosome was pointed in both species. The chromosomes of A. nuneztovari were larger than those of A. darlingi except for the Y chromosome. A. nuneztovari showed size polymorphism in the X chromosome. Somatic pairing and a strong constriction around the centromeric region in chromosomes II and III...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300010

Molecular Cytogenetics: : PCR-based diagnosis of human trisomies using computer-assisted laser densitometry
Provedor de dados: Genetics and Molecular Biology
Autores: Pena,Sérgio D.J.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300005

Noninvasive genetic sampling of endangered muriqui (Primates, Atelidae): efficiency of fecal DNA extraction
Provedor de dados: Genetics and Molecular Biology
Autores: Chaves,Paulo B.; Paes,Marcela F.; Mendes,Sérgio L.; Strier,Karen B.; Louro,Iúri D.; Fagundes,Valéria.
The muriqui (Brachyteles) is one of the most endangered primates in the world, however little is known about the viability of the remaining populations. We evaluated the technique of extracting DNA from wild muriqui feces for PCR applications. In order to determine the effect of the DNA in subsequent amplifications, we analyzed five different extracts. The importance of the recommended BSA and the HotStarTaq DNA polymerase was tested. The minimal conditions to successfully amplify highly degraded fecal DNA were determined, showing that the recommended reagents are not required. We envision that this method may be useful in further conservation management studies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachyteles; Conservation genetics; Endangered species; Fecal DNA; Noninvasive sampling.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400028

Haptoglobin gene subtypes in three Brazilian population groups of different ethnicities
Provedor de dados: Genetics and Molecular Biology
Autores: Miranda-Vilela,Ana L.; Akimoto,Arthur K.; Alves,Penha C.Z.; Hiragi,Cássia O.; Penalva,Guilherme C.; Oliveira,Silviene F.; Grisolia,Cesar K.; Klautau-Guimarães,Maria N.
Haptoglobin is a plasma hemoglobin-binding protein that limits iron loss during normal erythrocyte turnover and hemolysis, thereby preventing oxidative damage mediated by iron excess in the circulation. Haptoglobin polymorphism in humans, characterized by the Hp*1 and Hp*2 alleles, results in distinct phenotypes known as Hp1-1, Hp2-1 and Hp2-2, whose frequencies vary according to the ethnic origin of the population. The Hp*1 allele has two subtypes, Hp*1F and Hp*1S, that also vary in their frequencies among populations worldwide. In this work, we examined the distribution frequencies of haptoglobin subtypes in three Brazilian population groups of different ethnicities. The haptoglobin genotypes of Kayabi Amerindians (n = 56), Kalunga Afro-descendants (n =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian ethnicities; Haptoglobin; Polymorphism; Subtypes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300005

Genetic diversity of Hirsutella thompsonii isolates from Thailand based on AFLP analysis and partial beta-tubulin gene sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Tigano,Myrian S.; Adams,Byron; Maimala,Saowanit; Boucias,Drion.
Amplified fragment length polymorphism (AFLP) was used to investigate polymorphism among 43 Hirsutella thompsonii isolates (33 from Thailand) obtained from various mite species. The outgroups were an unidentified Hirsutella isolate along with Hirsutella nodulosa and Hirsutella kirchneri. Phylogenetic analyses of the AFLP data showed significant variation among isolates and the existence of three H. thompsonii clades. We also investigated the isolates using PCR with specific primers for the Hirsutella exotoxin gene Hirsutelin A (HtA) and 18 of these isolates were used for sequencing of the partial beta-tubulin gene. Phylogenetic analyses of beta-tubulin sequences showed two distinct H. thompsonii clades, one of which included AFLP clades I and II. For both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogenic fungus; Mites; Molecular markers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400022

Human mesenchymal stem cells are resistant to cytotoxic and genotoxic effects of cisplatin in vitro
Provedor de dados: Genetics and Molecular Biology
Autores: Bellagamba,Bruno Corrêa; Abreu,Bianca Regina Ribas de; Grivicich,Ivana; Markarian,Carolina Franke; Chem,Eduardo; Camassola,Melissa; Nardi,Nance Beyer; Dihl,Rafael Rodrigues.
Abstract Mesenchymal stem cells (MSCs) are known for their important properties involving multilineage differentiation potential., trophic factor secretion and localization along various organs and tissues. On the dark side, MSCs play a distinguished role in tumor microenvironments by differentiating into tumor-associated fibroblasts or supporting tumor growth via distinct mechanisms. Cisplatin (CIS) is a drug widely applied in the treatment of a large number of cancers and is known for its cytotoxic and genotoxic effects, both in vitro and in vivo. Here we assessed the effects of CIS on MSCs and the ovarian cancer cell line OVCAR-3, by MTT and comet assays. Our results demonstrated the resistance of MSCs to cell death and DNA damage induction by CIS,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mesenchymal stem cells; Cisplatin; MTT; Comet assay; OVCAR-3 cells.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100129

Genome-wide identification and characterization of R2R3-MYB genes in Medicago truncatula
Provedor de dados: Genetics and Molecular Biology
Autores: Li,Wei; Liu,Ying; Zhao,Jinyue; Zhen,Xin; Guo,Changhong; Shu,Yongjun.
Abstract MYB is a large family of plant transcription factors. Its function has been identified in several plants, while there are few reports in Medicago truncatula. In this study, we used RNA-seq data to analyze and identify R2R3-MYB genes in the genome of Medicago truncatula. Phylogenetic analysis classified 150 MtMYB genes into 21 subfamilies with homologs. Out of the 150 MtMYB genes, 139 were distributed among 8 chromosomes, with tandem duplications (TD) and segment duplications (SD). Microarray data were used for functional analysis of the MtMYB genes during growth and developmental processes providing evidence for a role in tissues differentiation, seed development processes, and especially the nodulation process. Furthermore, we investigated the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicago truncatula; MYB; Abiotic stress; QRT-PCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400611

Papillomaviruses: a systematic review
Provedor de dados: Genetics and Molecular Biology
Autores: Araldi,Rodrigo Pinheiro; Assaf,Suely Muro Reis; Carvalho,Rodrigo Franco de; Carvalho,Márcio Augusto Caldas Rocha de; Souza,Jacqueline Mazzuchelli de; Magnelli,Roberta Fiusa; Módolo,Diego Grando; Roperto,Franco Peppino; Stocco,Rita de Cassia; Beçak,Willy.
Abstract In the last decades, a group of viruses has received great attention due to its relationship with cancer development and its wide distribution throughout the vertebrates: the papillomaviruses. In this article, we aim to review some of the most relevant reports concerning the use of bovines as an experimental model for studies related to papillomaviruses. Moreover, the obtained data contributes to the development of strategies against the clinical consequences of bovine papillomaviruses (BPV) that have led to drastic hazards to the herds. To overcome the problem, the vaccines that we have been developing involve recombinant DNA technology, aiming at prophylactic and therapeutic procedures. It is important to point out that these strategies can be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine papillomavirus (BPV); Bovine papillomatosis; Carcinogenesis; Natural history.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100002

Multilocus DNA fingerprinting in paternity analysis: a Chilean experience
Provedor de dados: Genetics and Molecular Biology
Autores: Cifuentes O.,Lucía; Armanet B.,Leonor; Aguirre A.,Raúl; Vargas B.,Juana; Acuña P.,Mónica.
DNA polymorphism is very useful in paternity analysis. The present paper describes paternity studies done using DNA profiles obtained with the (CAC)5 probe. All of the subjects studied were involved in nonjudicial cases of paternity. Genomic DNA digested with HaeIII was run on agarose gels and hybridized in the gel with the (CAC)5 probe labeled with 32P. The mean number of bands larger than the 4.3 kb per individual was 16.1. The mean proportion of bands shared among unrelated individuals was 0.08 and the mean number of test bands was 7.1. This corresponded to an exclusion probability greater than 0.999999. Paternity was excluded in 34.5% of the cases. The mutation frequency estimated from non-excluded cases was 0.01143 bands per child. In these cases, the...
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400005

Cytogenetic data of Partamona peckolti (Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/CMA3 and DA/DAPI
Provedor de dados: Genetics and Molecular Biology
Autores: Brito,Rute Magalhães; Caixeiro,Ana Paula de Almeida; Pompolo,Silvia das Graças; Azevedo,Giselle Garcia.
The stingless bees of the Partamona genus have been studied taxonomically, ecologically and behaviourally, but cytogenetic studies are still rare. The objective of this study was to obtain cytogenetic data to contribute to Partamona peckolti species characterization. Heterochromatin was localized in all chromosome pericentromeric regions but some blocks could be visualized on some large chromosomes arms. A large heterozygous DA-CMA3-positive band was observed on one large chromosome arm, but was completely absent when C banding was applied before fluorochrome staining, with only one small positive band being visualized. Sequential DA-CMA3-NOR staining of interphase nuclei provided coincident positive responses. This suggests that DA-CMA3-positive bands of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meliponini; Partamona; Karyotype; C-banding; Fluorochromes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100009

Population genetic structure of Sisyrinchium micranthum Cav. (Iridaceae) in Itapuã State Park, Southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Tacuatiá,Luana Olinda; Eggers,Lilian; Kaltchuk-Santos,Eliane; Souza-Chies,Tatiana T.
Sisyrinchium micranthum Cav. is a member of the family Iridaceae, which is distributed over the American continent. In Brazil, this species is found, not only in disturbed areas and coastal regions, but is also very common in urban centers, such as public parks, during the spring. Chromosome counts for North American specimens are 2n = 32 and 2n = 48, whereas in southern Brazil, there is a polyploidy series with three chromosome numbers, 2n = 16, 2n = 32, and 2n = 48. Population analyses using DNA molecular markers are inexistent for this species, in spite of its wide distribution and morphological variation. To study the genetic population structure of S. micranthum, five natural populations were accessed in a conservation park within the Atlantic Rain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iridaceae; ISSR-PCR; Mating system; Population genetics; Sisyrinchium micranthum.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100014

Rare α0-thalassemia deletions detected by MLPA in five unrelated Brazilian patients
Provedor de dados: Genetics and Molecular Biology
Autores: Mota,Natália O.; Kimura,Elza M.; Ferreira,Roberta D.; Pedroso,Gisele A.; Albuquerque,Dulcinéia M.; Ribeiro,Daniela M.; Santos,Magnun N. N.; Bittar,Cristina M.; Costa,Fernando F.; Sonati,Maria de Fatima.
Abstract Alpha-thalassemias are among the most common genetic diseases in the world. They are characterized by hypochromic and microcytic anemia and great clinical variability, ranging from a practically asymptomatic phenotype to severe anemia, which can lead to intrauterine or early neonatal death. Deletions affecting the α-globin genes, located on chromosome 16p13.3, are the main causes of α-thalassemia. Multiplex ligation-dependent probe amplification (MLPA) can be used to detect rearrangements that cause α-thalassemia, particularly large deletions involving the whole α cluster and/or deletions in the HS-40 region. Here, MLPA was used to investigate the molecular basis of α-thalassemia in five unrelated patients, three of whom had Hb H disease. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-Thalassemia; Hb H disease; Multiplex ligation-dependent probe amplification; MLPA; Brazilian population.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500768

Large miRNA survival analysis reveals a prognostic four-biomarker signature for triple negative breast cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Andrade,Fernando; Nakata,Asuka; Gotoh,Noriko; Fujita,André.
Abstract Triple negative breast cancer (TNBC) is currently the only major breast tumor subtype without effective targeted therapy and, as a consequence, usually presents a poor outcome. Due to its more aggressive phenotype, there is an urgent clinical need to identify novel biomarkers that discriminate individuals with poor prognosis. We hypothesize that miRNAs can be used to this end because they are involved in the initiation and progression of tumors by altering the expression of their target genes. To identify a prognostic biomarker in TNBC, we analyzed the miRNA expression of a cohort composed of 185 patients diagnosed with TNBC using penalized Cox regression models. We identified a four-biomarker signature based on miR-221, miR-1305, miR-4708, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiRNA; MiR-221; MiR-1305; MiR-4708; RMDN2.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100110

Identifying water stress-response mechanisms in citrus by in silico transcriptome analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Torres,Gisele A.M.; Gimenes,Marcos A.; Rosa Jr.,Vicente E. de; Quecini,Vera.
Water deficit is one of the most critical environmental stresses to which plants are submitted during their life cycle. The evolutionary and economic performance of the plant is affected directly by reducing its survival in the natural environment and its productivity in agriculture. Plants respond to water stress with biochemical and physiological modifications that may be involved in tolerance or adaptation mechanisms. A great number of genes have been identified as transcriptionally regulated for water deficit. EST sequencing projects provide a significant contribution to the discovery of expressed genes. The identification and determination of gene expression patterns is important not only to understand the molecular bases of plant responses but also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Environmental stress; CitEST; Data mining; Tolerance mechanisms; Water deficit.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500018

American marsupials chromosomes: why study them?
Provedor de dados: Genetics and Molecular Biology
Autores: Svartman,Marta.
Marsupials, one of the three main groups of mammals, are only found in Australia and in the American continent. Studies performed in Australian marsupials have demonstrated the great potential provided by the group for the understanding of basic genetic mechanisms and chromosome evolution in mammals. Genetic studies in American marsupials are relatively scarce and cytogenetic data of most species are restricted to karyotype descriptions, usually without banding patterns. Nevertheless, the first marsupial genome sequenced was that of Monodelphis domestica, a South American species. The knowledge about mammalian genome evolution and function that resulted from studies on M. domestica is in sharp contrast with the lack of genetic data on most American...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marsupials; Cytogenetics; Chromosome evolution.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400002

Eucalyptus ESTs corresponding to the protoporphyrinogen IX oxidase enzyme related to the synthesis of heme, chlorophyll, and to the action of herbicides
Provedor de dados: Genetics and Molecular Biology
Autores: Velini,Edivaldo Domingues; Trindade,Maria Lúcia Bueno; Alves,Elza; Catâneo,Ana Catarina; Marino,Celso Luis; Maia,Ivan de Godoy; Mori,Edson Seizo; Furtado,Edson Luiz; Guerrini,Iraê Amaral; Wilcken,Carlos Frederico.
This work was aimed at locating Eucalyptus ESTs corresponding to the PROTOX or PPO enzyme (Protoporphyrinogen IX oxidase, E.C. 1.3.3.4) directly related to resistance to herbicides that promote oxidative stress, changing the functionality of this enzyme. PROTOX, which is the site of action of diphenyl-ether (oxyfluorfen, lactofen, fomesafen), oxadiazole (oxadiazon and oxadiargyl), and aryl triazolinone (sulfentrazone and carfentrazone) herbicides, acts on the synthesis route of porphyrins which is associated with the production of chlorophyll a, catalases, and peroxidases. One cluster and one single read were located, with e-values better than e-70, associated to PROTOX. The alignment results between amino acid sequences indicated that this enzyme is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus; Protoporphyrinogen IX oxidase; Catalase; Peroxidase; Herbicide.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400009

Association of the estrogen receptor gene Pvu II restriction polymorphism with expected progeny differences for reproductive and performance traits in swine herds in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Santana,Bárbara Amélia Aparecida; Biase,Fernando H.; Antunes,Robson Carlos; Borges,Mauricio; Franco,Mauricio Machaim; Goulart,Luiz Ricardo.
Estrogen has an important function in swine reproduction and growth. A Pvu II restriction enzyme polymorphism has been proven to be an important genetic variation in the estrogen receptor gene (ESR) and may be considered as a candidate gene for use in pig production but there is no data regarding the prevalence of this polymorphism in the Brazilian pig population. We used DNA samples from the following three purebred pig breeds: Large White (336 females and 26 males), Landrace (304 females and 27 males) and Pietrain (125 females and 11 males). The ESR genotyping was performed using PCR-RFLP. For each breed, genotypes for the ESR gene were compared independently for expected progeny differences (EPD) in litter size (LS), average daily weight gain (DWG)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ESR; Estrogen receptor; Pig.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200013

Embryology and cytogenetics of Eupatorium pauciflorum and E. intermedium (Compositae)
Provedor de dados: Genetics and Molecular Biology
Autores: Bertasso-Borges,Maristela Sanches; Coleman,James Robert.
The embryology of Eupatorium pauciflorum indicates diplospory with autonomous endosperm development. The embryo sac is of the polygonum type and the polar nuclei mostly fuse before anthesis. The occurrence of precocious embryo and endosperm development in unopened florets, and the total absence of germinated pollen grains on exposed stigmas, as well as the absence of pollen tubes in the ovules, indicate agamospermy to be obligate and embryo and endosperm development autonomous. The study of microsporogenesis revealed the total absence of pollen production in consequence of microsporocyte degeneration before the onset of meiosis, which resulted in absolute male sterility. E. pauciflorum was demonstrated to be an autotriploid with a basic set of 10...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400017

Population analysis of a chromosome polymorphism in Astyanax (Teleostei, Characiformes) species endemic to the Iguaçu River
Provedor de dados: Genetics and Molecular Biology
Autores: Kantek,Daniel Luis Zanella; Cipriano,Roger Raupp; Noleto,Rafael Bueno; Fenocchio,Alberto Sergio; Artoni,Roberto Ferreira; Cestari,Marta Margarete.
Chromosomal analyses were performed in the fish Astyanax sp.D collected from three different points: two streams from the right bank and one from the left bank of the Upper Iguaçu River, Paraná State, Brazil. The individuals from all localities possess 2n = 50 chromosomes and a FN = 84 (4m+24sm+6st+16a). The C-banding pattern was similar in all populations. However, within each population, an interindividual variation concerning the number and localization of heterochromatic bands was observed. Some of these variations were quantified in each population, and the results indicate that the samples were not different when studying the variable frequencies. Considering that Astyanax sp.D is typical in the headwaters of the Iguaçu River, these results were not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population cytogenetics; Chromosome variations; Astyanax fish.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200013

Inheritance and identification of molecular markers associated with spot blotch (Cochliobolus sativus L.) resistance through microsatellites analysis in barley
Provedor de dados: Genetics and Molecular Biology
Autores: Kuldeep,Tyagi; Nandan,Rajiv; Kumar,Uttam; Prasad,Lal Chand; Chand,Ramesh; Joshi,Arun Kumar.
Spot blotch resistant (IBON 18) and susceptible (RD 2508) lines were crossed to investigate inheritance of resistance and to identify simple sequence repeats (SSRs) associated with resistance. F1 resistance was intermediate and suggested additive nature of inheritance. Three additive genes was noted in the distribution of F3, F4 and F5 generations. In F6 and F6-7, the quantitative and qualitative approaches also suggested the control of three resistance genes. The parents and the RILs (F6/F6-7) were grown in four environments and spot blotch severity recorded. Forty five SSR primers, specific for chromosomes 1 (7H) and 5 (1H), were applied. Of these, 12 were polymorphic between the parents, and between the resistant and susceptible bulks. Three markers BMS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite markers; Molecular marker; Spot blotch; Cochliobolus sativus; Barley.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400021

Chemical composition and antigenotoxic properties of Lippia alba essential oils
Provedor de dados: Genetics and Molecular Biology
Autores: López,Molkary Andrea; Stashenko,Elena E.; Fuentes,Jorge Luis.
The present work evaluated the chemical composition and the DNA protective effect of the essential oils (EOs) from Lippia alba against bleomycin-induced genotoxicity. EO constituents were determined by Gas Chromatography/Mass Spectrometric (GC-MS) analysis. The major compounds encountered being citral (33% geranial and 25% neral), geraniol (7%) and trans-β-caryophyllene (7%) for L. alba specimen COL512077, and carvone (38%), limonene (33%) and bicyclosesquiphellandrene (8%) for the other, COL512078. The genotoxicity and antigenotoxicity of EO and the compounds citral, carvone and limonene, were assayed using the SOS Chromotest in Escherichia coli. The EOs were not genotoxic in the SOS chromotest, but one of the major compound (limonene) showed genotoxicity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lippia alba; Essential oil; Antigenotoxicity; Bleomycin; SOS chromotest.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300019

Genotypic diversity of the Killer Cell Immunoglobulin-like Receptors (KIR) and their HLA class I Ligands in a Saudi population
Provedor de dados: Genetics and Molecular Biology
Autores: Omar,Suliman Y. Al; Alkuriji,Afrah; Alwasel,Saleh; Dar,javid Ahmed; Alhammad,Alwaleed; Christmas,Stephen; Mansour,Lamjed.
Abstract Killer Cell Immunoglobulin-like Receptors (KIR) have been used as good markers for the study of genetic predisposition in many diseases and in human genetic population dynamics. In this context, we have investigated the genetic diversity of KIR genes and their main HLA class I ligands in Saudi population and compared the data with other studies of neighboring populations. One hundred and fourteen randomly selected healthy Saudi subjects were genotyped for the presence or absence of 16 KIR genes and their HLA-C1, -C2, -Bw4Thr80 and Bw4Ile80 groups, using a PCR-SSP technique. The results show the occurrence of the framework genes (3DL2, 3DL3 and 2DL4) and the pseudogenes (2DP1 and 3DP1) at highest frequencies. All inhibitory KIR (iKIR) genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: KIR diversity; Gene polymorphism; Molecular evolution; PCR SSP; Population viability analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100014

Effects of CSN3 and LGB gene polymorphisms on production traits in beef cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Curi,Rogério A.; Oliveira,Henrique N. de; Gimenes,Marcos A.; Silveira,Antonio C.; Lopes,Catalina R.
The objective of the present study was to estimate the allele and genotype frequencies of the CSN3/HinfI and LGB/HaeIII gene polymorphisms in beef cattle belonging to different genetic groups, and to determine the effects of these polymorphisms on growth and carcass traits in these animals, which are submitted to an intensive production model. Genotyping was performed on 79 Nelore, 30 Canchim (5/8 Charolais + 3/8 Zebu) and 275 crossbred cattle originating from the crosses of Simmental (n = 30) and Angus (n = 245) sires with Nelore females. Body weight, weight gain, dressing percentage, longissimus dorsi area and backfat thickness were fitted using the GLM procedure, and least square means of the genotypes were compared by the F test. The results showed...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Beef cattle; Polymorphisms; Candidate gene; Growth; Carcass.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200015

Cloning and expression analysis of the chitinase gene Ifu-chit2 from Isaria fumosorosea
Provedor de dados: Genetics and Molecular Biology
Autores: Meng,Huimin; Wang,Zhangxun; Meng,Xiangyun; Xie,Ling; Huang,Bo.
Entomopathogenic fungi can produce a series of chitinases, some of which function synergistically with proteases and other hydrolytic enzymes to degrade the insect cuticle. In the present study, the chitinase gene Ifu-chit2 from Isaria fumosorosea was investigated. The Ifu-chit2 gene is 1,435-bp long, interrupted by three short introns, and encodes a predicted protein of 423 amino acids with a 22 residue signal peptide. The predicted Ifu-Chit2 protein is highly homologous to Beauveria bassiana chitinase Bbchit2 and belongs to the glycohydrolase family 18. Ifu-Chit2 was expressed in Escherichia coli to verify chitinase activity, and the recombinant enzyme exhibited activity with a colloidal chitin substrate. Furthermore, the expression profiles of Ifu-chit2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isaria fumosorosea; Chitinase; Prokaryotic expression; Quantitative real-time PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300381

Heat shock-induced apoptosis in germ line cells of Triatoma infestans Klug
Provedor de dados: Genetics and Molecular Biology
Autores: Mello,Maria Luiza S.; Maria,Sílvya S.; Tavares,Maria Cristina H.
The survival of Triatoma infestans and of somatic cells from this species is affected by heat shock. In this study, we examined the cell death responses of male germ line cells from 5th instar nymphs 7 and 30 days after heat shock exposure (40ºC, 1 h). The preparations were stained by the Feulgen reaction and the TUNEL immunocytochemical assay. Apoptosis was elicited by heat shock, with positive TUNEL responses in spermatogonial chromatin and chromosomes, spermatids and sperm cells. Spermatogonia were most affected seven days after the shock whereas some spermatids and sperm cells exhibited DNA fragmentation only thirty days after heat shock. The rate of cell death varied among the cells. In some cases, cellular differentiation was unaffected by heat...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200011

Clustering and artificial neural networks: classification of variable lengths of Helminth antigens in set of domains
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Thiago de Souza; Pacífico,Lucila Grossi Gonçalves; Teixeira,Santuza Maria Ribeiro; Oliveira,Sérgio Costa; Braga,Antônio de Pádua.
A new scheme for representing proteins of different lengths in number of amino acids that can be presented to a fixed number of inputs Artificial Neural Networks (ANNs) speel-out classification is described. K-Means's clustering of the new vectors with subsequent classification was then possible with the dimension reduction technique Principal Component Analysis applied previously. The new representation scheme was applied to a set of 112 antigens sequences from several parasitic helminths, selected in the National Center for Biotechnology Information and classified into fourth different groups. This bioinformatic tool permitted the establishment of a good correlation with domains that are already well characterized, regardless of the differences between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics; Artificial neural networks; Clustering; Helminth antigen; Domain.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400032

Mendelian breeding units versus standard sampling strategies: mitochondrial DNA variation in southwest Sardinia
Provedor de dados: Genetics and Molecular Biology
Autores: Sanna,Daria; Pala,Maria; Cossu,Piero; Dedola,Gian Luca; Melis,Sonia; Fresu,Giovanni; Morelli,Laura; Obinu,Domenica; Tonolo,Giancarlo; Secchi,Giannina; Triunfo,Riccardo; Lorenz,Joseph G.; Scheinfeldt,Laura; Torroni,Antonio; Robledo,Renato; Francalacci,Paolo.
We report a sampling strategy based on Mendelian Breeding Units (MBUs), representing an interbreeding group of individuals sharing a common gene pool. The identification of MBUs is crucial for case-control experimental design in association studies. The aim of this work was to evaluate the possible existence of bias in terms of genetic variability and haplogroup frequencies in the MBU sample, due to severe sample selection. In order to reach this goal, the MBU sampling strategy was compared to a standard selection of individuals according to their surname and place of birth. We analysed mitochondrial DNA variation (first hypervariable segment and coding region) in unrelated healthy subjects from two different areas of Sardinia: the area around the town of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breeding units strategy; MtDNA haplogroup distribution; Association studies.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200003

Induction of SCEs and DNA fragmentation in bovine peripheral lymphocytes by in vitro exposure to tolylfluanid-based fungicide
Provedor de dados: Genetics and Molecular Biology
Autores: Siviková,Katarína; Dianovsky,Ján; Holecková,Beáta.
The potential for genotoxic and cytotoxic effects of tolylfluanid-based fungicide (50% active agent) was evaluated using sister chromatid exchange (SCE) and proliferation indices (PI) in cultured bovine peripheral lymphocytes. For the detection of possible genetic damage, DNA fragmentation assay was also applied. Bovine lymphocytes cultured for 72 h were treated with the fungicide at the final concentrations of 1.75, 3.5, 8.75, and 17.5 µg/mL for the last 24 and 48 h of culture without S9 metabolic activation, and during the last 2 h of culture with S9 metabolic activation. In the SCE assays no evidence for genotoxic activity of the fungicide was found in treatments of 24 h without and 2 h with S9. After the 24 h exposure to tolylfluanid, a weak decrease...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tolylfluanid-based fungicide; Sister chromatid exchanges; DNA fragmentation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100019

Bias in the prediction of genetic gain due to mass and half-sib selection in random mating populations
Provedor de dados: Genetics and Molecular Biology
Autores: Viana,José Marcelo Soriano; Faria,Vinícius Ribeiro; Silva,Admilson da Costa e.
The prediction of gains from selection allows the comparison of breeding methods and selection strategies, although these estimates may be biased. The objective of this study was to investigate the extent of such bias in predicting genetic gain. For this, we simulated 10 cycles of a hypothetical breeding program that involved seven traits, three population classes, three experimental conditions and two breeding methods (mass and half-sib selection). Each combination of trait, population, heritability, method and cycle was repeated 10 times. The predicted gains were biased, even when the genetic parameters were estimated without error. Gain from selection in both genders is twice the gain from selection in a single gender only in the absence of dominance....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Predicted genetic gain; Realized genetic gain; Recurrent selection.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300012

Analysis of genetic susceptibility to mercury contamination evaluated through molecular biomarkers in at-risk Amazon Amerindian populations
Provedor de dados: Genetics and Molecular Biology
Autores: Klautau-Guimarães,Maria de Nazare; D’Ascenção,Renata; Caldart,Fabiana A.; Grisolia,Cesar K.; Souza,Jurandir R. de; Barbosa,Antonio C.; Cordeiro,Célia M.T.; Ferrari,Iris.
We investigated Individual differences in susceptibility to methylmercury (MeHg) contamination and its relationship with polymorphisms of the detoxifying enzyme glutathione S-transferase (GST). In Brazil, some Amerindian tribes from the Amazon region have an increased level of mercury in their hair. Samples of hair and blood were taken from inhabitants of two villages in the Kayabi and Munduruku Amerindian communities to investigate mercury levels in association with genetic polymorphism of GSTs. Other molecular biological markers were also studied, such as hemoglobin, haptoglobin and glucose 6-phosphate dehydrogenase (G-6-PDH). Higher levels of mercury contamination were found in the Kayabi villagers, who had a null genotype (GSTM1 0/0, also denominated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amerindian; Genetic polymorphism; Glutathione S-transferase; Mercury.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500027

A rare case of Acute Lymphocytic Leukemia (ALL) presenting with double Philadelphia chromosome: relapse or secondary leukemia?
Provedor de dados: Genetics and Molecular Biology
Autores: Campos,Mireille Guimarães Vaz de; Chauffaille,Maria de Lourdes L. Ferrari; Rodrigues,Celso Arrais; Krum,Everson A.; Yamamoto,Mihoko.
The Philadelphia chromosome is observed in 5% of pediatric acute lymphocytic leukemia (ALL) and in 25% to 50% of adult ALL cases, and is associated with poor prognosis. Double Ph in a hyperdiploid karyotype is common in chronic myeloid leukemia (CML), but rarely found in ALL. We report here the case of a girl diagnosed with ALL at 7 years of age. After treatment with the pediatric protocol BFM 83 for ALL, she stayed in continuous complete remission for nine years. At age 19, she was re-admitted with a white blood cell count of 6.8 x 10(9)/L with 3% blasts, and a platelet count of 65 x 109/L. Bone marrow aspirate showed 92.6% lymphoid blast cells, and chromosome analysis after G-banding revealed the karyotype...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Relapsed acute lymphocytic leukemia; Double Philadelphia chromosome.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300006

Penetrance rate estimation in autosomal dominant conditions
Provedor de dados: Genetics and Molecular Biology
Autores: Otto,Paulo A.; Horimoto,Andréa R.V.R.
Accurate estimates of the penetrance rate of autosomal dominant conditions are important, among other issues, for optimizing recurrence risks in genetic counseling. The present work on penetrance rate estimation from pedigree data considers the following situations: 1) estimation of the penetrance rate K (brief review of the method); 2) construction of exact credible intervals for K estimates; 3) specificity and heterogeneity issues; 4) penetrance rate estimates obtained through molecular testing of families; 5) lack of information about the phenotype of the pedigree generator; 6) genealogies containing grouped parent-offspring information; 7) ascertainment issues responsible for the inflation of K estimates.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penetrance rate; Maximum likelihood method; Recurrence risks; Genetic counseling.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400006

Genetic structure of natural Eugenia dysenterica DC (Myrtaceae) populations in northeastern Goiás, Brazil, accessed by morphological traits and RAPD markers
Provedor de dados: Genetics and Molecular Biology
Autores: Trindade,Maria da Glória; Chaves,Lázaro José.
The Brazilian Cerrado (Savannah) is a biome with great biodiversity. Many of the species found in this biome have unlimited economic potential and are used by the local population for various purposes. With the opening of the agricultural frontiers in this region, much of the native flora has been replaced by extensive areas of monocultures. Thirteen populations of the Eugenia dysenterica DC species were sampled in the northeast of the state of Goiás, Brazil, to analyze their genetic structure. Morphological traits were measured for all the progeny obtained and some of them were analyzed by using RAPD markers. The natural populations of the species showed high levels of variability for the morphological traits. The phenotypic, genotypic and genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado; Cagaiteira; Population genetics; Genetic structure and conservation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300013

Chromosomal banding patterns in the eyelid-less microteiid lizard radiation: The X1X1X2X2:X1X2Y sex chromosome system in Calyptommatus and the karyotypes of Psilophthalmus and Tretioscincus (Squamata, Gymnophthalmidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Yonenaga-Yassuda,Yatiyo; Rodrigues,Miguel Trefaut; Pellegrino,Katia Cristina Machado.
Cytogenetic studies were performed on five closely related microteiid lizards (Gymnophthalmini), three Calyptommatus species and Psilophthalmus paeminosus from the sand dunes of the middle São Francisco river in the semiarid caatinga of the Brazilian state of Bahia and Tretioscincus oriximinensis from the Brazilian Amazon region. The diploid chromosome number in Calyptommatus species was 2n = 58 in females and 2n = 57 in males due to a multiple X1X1X2X2:X1X2Y sex chromosomes system, while P. paeminosus was 2n = 44 (20M+24m): where M = macrochromosomes and m = microchromosomes) and T. oriximinensis 2n = 42 (18M+24m). A single pair of silver staining nucleolar organizing regions (Ag-NORs) characterizes all five species. Incorporation of 5-BrdU...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gymnophthalmidae; Banding patterns; Karyotypes; Multiple sex chromosomes.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500009

Expression analysis on 14-3-3 proteins in regenerative liver following partial hepatectomy
Provedor de dados: Genetics and Molecular Biology
Autores: Xue,Deming; Xue,Yang; Niu,Zhipeng; Guo,Xueqiang; Xu,Cunshuan.
Abstract 14-3-3 proteins play a vital part in the regulation of cell cycle and apoptosis as signaling integration points. During liver regeneration, the quiescent hepatocytes go through hypertrophy and proliferation to restore liver weight. Therefore, we speculated that 14-3-3 proteins regulate the progression of liver regeneration. In this study, we analyzed the expression patterns of 14-3-3 proteins during liver regeneration of rat to provide an insight into the regenerative mechanism using western blotting. Only four isoforms (γ, ε, σ and τ/θ) of the 14-3-3 proteins were expressed in regenerative liver after partial hepatectomy (PH). The dual effects, the significant down-regulation of 14-3-3ε and the significant up-regulation of 14-3-3τ/θ at 2 h after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver regeneration; 14-3-3 proteins; Western blotting.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500855

Non-random pre-transcriptional evolution in HIV-1: a refutation of the foundational conditions for neutral evolution
Provedor de dados: Genetics and Molecular Biology
Autores: Valenzuela,Carlos Y.
The complete base sequence of HIV-1 virus and GP120 ENV gene were analyzed to establish their distance to the expected neutral random sequence. An especial methodology was devised to achieve this aim. Analyses included: a) proportion of dinucleotides (signatures); b) homogeneity in the distribution of dinucleotides and bases (isochores) by dividing both segments in ten and three sub-segments, respectively; c) probability of runs of bases and No-bases according to the Bose-Einstein distribution. The analyses showed a huge deviation from the random distribution expected from neutral evolution and neutral-neighbor influence of nucleotide sites. The most significant result is the tremendous lack of CG dinucleotides (p &lt; 10-50), a selective trait of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-random evolution; HIV-1; Bose-Einstein distribution; Pre-transcriptional evolution; Non-random sequences.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100025

Identification of sugarcane cDNAs encoding components of the cell cycle machinery
Provedor de dados: Genetics and Molecular Biology
Autores: Andrietta,Mírian Helene; Eloy,Núbia Barbosa; Hemerly,Adriana Silva; Ferreira,Paulo C.G.
Data on cell cycle research in plants indicate that the majority of the fundamental regulators are conserved with other eukaryotes, but the controlling mechanisms imposed on them, and their integration into growth and development is unique to plants. To date, most studies on cell division have been conducted in dicot plants. However, monocot plants have distinct developmental strategies that will affect the regulation of cell division at the meristems. In order to advance our understanding how cell division is integrated with the basic mechanisms controlling cell growth and development in monocots, we took advantage of the sugarcane EST Project (Sucest) to carry an exhaustive data mining to identify components of the cell cycle machinery. Results obtained...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100010

Karyotype, constitutive heterochromatin and nucleolar organizer regions (NORs) in Belosacris coccineipes (Acrididae-Leptysminae)
Provedor de dados: Genetics and Molecular Biology
Autores: Loreto,Vilma; Souza,Maria José de.
Several techniques including C-banding, fluorochromes and silver staining were used to obtain information about heterochromatin patterns in the grasshopper B. coccineipes. Conventional staining showed a karyotype with 2n = 23 chromosomes in males and 2n = 24 in females, as well as XO:XX sex determination and acrotelocentric chromosomes. The medium-sized X chromosome was heteropycnotic positive at the beginning of prophase I and negative in metaphase I. C-banding revealed heterochromatic blocks in the pericentromeric regions of all chromosomes. Silver nitrate staining in this species showed three small bivalents (S9-S11) as nucleolar organizers with NORs located in the pericentromeric regions. CMA3-positive blocks were seen in pericentromeric regions of...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300013

Prediction of the amount of secondary structure of proteins using unassigned NMR spectra: a tool for target selection in structural proteomics
Provedor de dados: Genetics and Molecular Biology
Autores: Moreau,Vitor Hugo; Valente,Ana Paula; Almeida,Fábio C.L.
With the advent of structural genomics, the need for fast structural information about unknown proteins has increased. We describe a new methodology, based on 13C, 15N and ¹H chemical shift dispersion to predict the amount of secondary structure of unassigned proteins from their 15N- and/or 13C-edited heteronuclear single quantum coherence (HSQC) spectra. This methodology has been coded into a software called PASSNMR (Prediction of the Amount of Secondary Structure by Nuclear Magnetic Resonance), which can be accessed directly from the Internet. PASSNMR program is a powerful tool for screening proteins for proteomic or structural genomic investigations when used with recent methodologies that take advantage of the use of the antibiotic rifampicin to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemical shift dispersion; PASSNMR; Prediction of secondary structure; Structural proteomics; Target selection.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400030

Genetic control of the fungus Colletotrichum lindemuthianum (Sacc. &amp;amp; Magn.) Scrib. reaction and corona color in the common bean (Phaseolus vulgaris L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Mendonça,H.A. de; Santos,J.B. dos; Ramalho,M.A.P.; Ferreira,D.F.
An important trait for common bean (Phaseolus vulgaris L.) cultivars with Carioca type grain is resistance to Colletotrichum lindemuthianum, which causes anthracnose and a pale corona. The present study was conducted to understand the genetic control of common bean reaction to the fungus and of the corona color, to provide guides to future breeding studies. Genotypes P-45, with brown corona, and EMGOPA 201-Ouro, with yellow corona, are resistant to C. lindemuthianum. Cultivar Carioca is susceptible to anthracnose, but it has desirable grain and corona color. Anthracnose resistance and corona color were studied in the F1 and F2 generations of three populations resulting from crosses of P-45, EMGOPA 201-Ouro, and Carioca. The Carioca x P-45 cross indicated...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300008

The systemic concept of the gene, at age fifteen, and comments on C.N. El-Hani's article 'Between the cross and the sword: the crisis of the gene concept'
Provedor de dados: Genetics and Molecular Biology
Autores: Guimarães,Romeu Cardoso.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400033

Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts
Provedor de dados: Genetics and Molecular Biology
Autores: Pacheco,Flávia Teresa Hansen; Silva-Stenico,Maria Estela; Etchegaray,Augusto; Gomes,José Elias; Carrilho,Emanuel; Tsai,Siu Mui.
Bacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa strain 9a5c. However, a search for siderophore-related genes in strain 9a5c indicated five membrane receptors, including siderophore, ferrichrome-iron and hemin receptors. All these biomolecules are thought to be associated with iron transport and utilization. Eighty isolates obtained from citrus orchards containing trees that developed citrus variegated chlorosis (CVC) were screened for siderophore production. The results demonstrated that only 10 of the isolates did not produce siderophores. Additional strains obtained from coffee, almond, mulberry, elm,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus variegated chlorosis; Plant pathogen; Iron-transportation; Pyoverdine; Enterobactin; Polyketide synthase; Peptide synthetase.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100026

Oxytocin and arginine vasopressin systems in the domestication process
Provedor de dados: Genetics and Molecular Biology
Autores: Fam,Bibiana S.O.; Paré,Pamela; Felkl,Aline B.; Vargas-Pinilla,Pedro; Paixão-Côrtes,Vanessa R.; Viscardi,Lucas Henriques; Bortolini,Maria Cátira.
Abstract Domestication is of unquestionable importance to the technological revolution that has given rise to modern human societies. In this study, we analyzed the DNA and protein sequences of six genes of the oxytocin and arginine vasopressin systems (OXT-OXTR; AVP-AVPR1a, AVPR1b and AVPR2) in 40 placental mammals. These systems play an important role in the control of physiology and behavior. According to our analyses, neutrality does not explain the pattern of molecular evolution found in some of these genes. We observed specific sites under positive selection in AVPR1b (ω = 1.429, p = 0.001) and AVPR2 (ω= 1.49, p = 0.001), suggesting that they could be involved in behavior and physiological changes, including those related to the domestication...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxytocin and receptors; Vasopressin and receptors; Animal domestication; Molecular evolution; Positive selection.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200235

Application of a double-enrichment procedure for microsatellite isolation and the use of tailed primers for high throughput genotyping
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz,Fábio Mendonça; Iyengar,Arati; Lima,Paulo Sarmanho da Costa; Maclean,Norman; Bentzen,Paul.
The number of microsatellite loci and their allelic diversity contribute to increase accuracy and informativity of genetic estimates, however, the isolation of microsatellite loci is not only laborious but also quite expensive. We used (GATA)n and (GACA)n tetranucleotide probes and single- and double-enrichment hybridization to construct and screen a genomic library with an increased proportion of DNA fragments containing repeat motifs. Repeats were found using both types of hybridization but the double-enrichment procedure recovered sequences of which 100% contained (GATA)n and (GACA)n motifs. Microsatellite loci primers were then designed with an M13R-tail or CAG-tag to produce scorable PCR products with minimal stutter. The approach used in this study...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Double-enrichment; Double-hybridization; Stutter bands; Tailed microsatellite primers.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300014

Null expectation of spatial correlograms under a stochastic process of genetic divergence with small sample sizes
Provedor de dados: Genetics and Molecular Biology
Autores: Telles,Mariana Pires de Campos; Diniz-Filho,José Alexandre Felizola.
An Ornstein-Uhlenbeck process was used to simulate the exponential relationship between genetic divergence and geographic distances, as predicted by stochastic processes of population differentiation, such as isolation-by-distance, stepping-stone or coalescence models. These simulations were based only on the spatial coordinates of the local populations that defined a spatial unweighted pair-group method using arithmetic averages (UPGMA) link among them. The simulated gene frequency surfaces were then analyzed using spatial autocorrelation procedures and Nei's genetic distances, constructed with different numbers of variables (gene frequencies). Stochastic divergence in space produced strong spatial patterns at univariate and mutivariate levels. Using a...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400007

Cytogenetics of species of the families Pimelodidae and Rhamdiidae (Siluriformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Swarça,Ana Cláudia; Caetano,Lucia Giuliano; Dias,Ana Lúcia.
Only 33 species among about 300 belonging to the families Pimelodidae and Rhamdiidae have been studied cytogenetically. The diploid number varies from 2n = 46 to 2n = 63 chromosomes, with the karyotypes often being of the meta/submetacentric type. As a result, there is generally a very elevated fundamental number.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300015

Assessing residual leukemia through fluorescence in situ hybridization
Provedor de dados: Genetics and Molecular Biology
Autores: Varella-Garcia,Marileila.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300006

Characterization and mapping of a spotted leaf mutant in rice (Oryza sativa)
Provedor de dados: Genetics and Molecular Biology
Autores: Xu,Xue; Zhang,Lili; Liu,Binmei; Ye,Yafeng; Wu,Yuejin.
Spotted leaf mutant belongs to a class of mutants that can produce necrotic lesions spontaneously in plants without any attack by pathogens. These mutants have no beneficial effect on plant productivity but provide a unique opportunity to study programmed cell death in plant defense responses. A novel rice spotted leaf mutant (spl30) was isolated through low-energy heavy ion irradiation. Lesion expression was sensitive to light and humidity. The spl30 mutant caused a decrease in chlorophyll and soluble protein content, with marked accumulation of reactive oxygen species (ROS) around the lesions. In addition, the spl30 mutant significantly enhanced resistance to rice bacterial blight (X. oryzae pv. oryzae) from China (C1-C7). The use of SSR markers showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene mapping; Reactive oxygen species; Rice (Orzya sativa); Rice bacterial blight; Spotted leaf mutant.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300013

Occupational exposure of workers to pesticides: Toxicogenetics and susceptibility gene polymorphisms
Provedor de dados: Genetics and Molecular Biology
Autores: Adad,Lúcia Maria de Miranda; Andrade,Heloísa Helena Rodrigues de; Kvitko,Kátia; Lehmann,Mauricio; Cavalcante,Ana Amélia de Carvalho Melo; Dihl,Rafael Rodrigues.
Farm workers are often exposed to pesticides, which are products belonging to a specific chemical group that affects the health of agricultural workers and is mostly recognized as genotoxic and carcinogenic. The exposure of workers from Piauí, Brazil, to these hazardous chemicals was assessed and cytogenetic alterations were evaluated using the buccal micronucleus assay, hematological and lipid parameters, butyrylcholinesterase (BChE) activity and genetic polymorphisms of enzymes involved in the metabolism of pesticides, such as PON1, as well as of the DNA repair system (OGG1, XRCC1 and XRCC4). Two groups of farm workers exposed to different types of pesticides were evaluated and compared to matched non-exposed control groups. A significant increase was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human monitoring; Buccal micronucleus test; Pesticides; PON 1; DNA repair genes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300308

Programmed cell death 1 gene (PDCD1) polymorphism and pemphigus foliaceus (fogo selvagem) disease susceptibility
Provedor de dados: Genetics and Molecular Biology
Autores: Braun-Prado,Karin; Petzl-Erler,Maria Luiza.
Pemphigus foliaceus, also known as fogo selvagem, is an autoimmune disease of the epidermis characterized by superficial blisters and antibodies against desmoglein 1. It is a multifactorial disease and genetic susceptibility is oligogenic or polygenic. Considering the crucial function of the programmed cell death 1 molecule (PD-1) in the immune response, the aim of this study was to verify if variants of the PDCD1 gene influence susceptibility and resistance to pemphigus foliaceus, in a case - control disease association study. We analyzed patients (n = 154) and unaffected control individuals (n = 325) of the Brazilian population, in respect to the PD1.3(G,A) PD1.5(C,T) and PD1.6(A,G) single nucleotide polymorphisms (SNPs) and also investigated, for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic association; PD-1; PDCD1; Genetic polymorphism; Pemphigus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300003

Comparing a single observation with a larger sample through Levene's test
Provedor de dados: Genetics and Molecular Biology
Autores: Cerqueira,Rui.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200012

Sucrose synthase molecular marker associated with sugar content in elite sugarcane progeny
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Jorge A. da; Bressiani,Jose A.
We describe the development and application of an expressed sequence tag (EST)-derived restriction fragment length polymorphism (RFLP) marker for sugarcane elite genotypes which can be used for quantitative trait loci (QTL) tagging for sugar content. EST-derived RFLP markers for proteins involved in sucrose metabolism have been used in Southern analysis for mapping and gene tagging in elite sugarcane clones. A single dose marker, obtained from a sucrose synthase EST associated with sugar content at the alpha = 0.01 probability level, is presented for sugarcane breeding. Utilization of EST homologues to known genes for generation of molecular markers accelerated the identification of a QTL controlling an important trait-sugar content. Sugarcane bacterial...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sucrose; Synthase; Expressed; Sequence; Tag.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200020

Genetic components affecting embryonic developmental time of Drosophila melanogaster
Provedor de dados: Genetics and Molecular Biology
Autores: Nascimento,Jurema Cruz do; Cruz,Ivana Beatrice Mânica da; Monjeló,Luiz Alberto; Oliveira,Alice Kalisz de.
The developmental time of the embryonic stage of Drosophila melanogaster was 21.66% faster and 14.75% slower than controls in populations selected for fast and slow developmental speed, respectively. The genetic model with two main loci with dominant and additive effect added to maternal effect and their relevant interactions can explain 96% of the phenotypic variability in the embryonic developmental time according to 14 crossing progenies involving fast and slow flies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Developmental rate; Embryo; Drosophila.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200008

Biochemical comparison of two Hypostomus populations (Siluriformes, Loricariidae) from the Atlântico Stream of the upper Paraná River basin, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Ito,Kennya F.; Renesto,Erasmo; Zawadzki,Cláudio H.
Two syntopic morphotypes of the genus Hypostomus - H. nigromaculatus and H. cf. nigromaculatus (Atlântico Stream, Paraná State) - were compared through the allozyme electrophoresis technique. Twelve enzymatic systems (AAT, ADH, EST, GCDH, G3PDH, GPI, IDH, LDH, MDH, ME, PGM and SOD) were analyzed, attributing the score of 20 loci, with a total of 30 alleles. Six loci were diagnostic (Aat-2, Gcdh-1, Gpi-A, Idh-1, Ldh-A and Mdh-A), indicating the presence of interjacent reproductive isolation. The occurrence of few polymorphic loci acknowledge two morphotypes, with heterozygosity values He = 0.0291 for H. nigromaculatus and He = 0.0346 for H. cf. nigromaculatus. F IS statistics demonstrated fixation of the alleles in the two morphotypes. Genetic identity (I)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allozymes; Hypostomus nigromaculatus; Fish genetics; Genetic distance; Polymorphism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100008

Comparison of the effectiveness of microsatellites and SNP panels for genetic identification, traceability and assessment of parentage in an inbred Angus herd
Provedor de dados: Genetics and Molecular Biology
Autores: Fernández,María E.; Goszczynski,Daniel E.; Lirón,Juan P.; Villegas-Castagnasso,Egle E.; Carino,Mónica H.; Ripoli,María V.; Rogberg-Muñoz,Andrés; Posik,Diego M.; Peral-García,Pilar; Giovambattista,Guillermo.
During the last decade, microsatellites (short tandem repeats or STRs) have been successfully used for animal genetic identification, traceability and paternity, although in recent year single nucleotide polymorphisms (SNPs) have been increasingly used for this purpose. An efficient SNP identification system requires a marker set with enough power to identify individuals and their parents. Genetic diagnostics generally include the analysis of related animals. In this work, the degree of information provided by SNPs for a consanguineous herd of cattle was compared with that provided by STRs. Thirty-six closely related Angus cattle were genotyped for 18 STRs and 116 SNPs. Cumulative SNPs exclusion power values (Q) for paternity and sample matching...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite; Single nucleotide polymorphism; Exclusion probability; Genetic identification; Bovine.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200008

Combining ability analysis for grain filling duration and yield traits in spring wheat (Triticum aestivum L. em. Thell.)
Provedor de dados: Genetics and Molecular Biology
Autores: Kamaluddin,; Singh,Rishi M.; Prasad,Lal C.; Abdin,Malik Z.; Joshi,Arun K.
A diallel analysis of wheat (Triticum aestivum L. em. Thell) parents (n = 11) and their F1 (n = 55) and F2 (n = 55) offspring was carried out for the following four traits: grain filling duration (GFD), GFD for growing degree days (GDD), 1000 seed weight and seed yield per plant. Analysis of variance for general combining ability (GCA) and specific combining ability (SCA) displayed significant F1 and F2 general and specific combining ability effects for the four traits studied. For all the traits the GCA effects were relatively more important than the SCA effects, indicating that additive genetic effects were predominant. Crosses displaying high SCA effects for grain filling duration, seed weight and yield were observed to be derived from parents having...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; Combining ability; GCA; SCA; Grain filling duration.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300018

Genetic variability in Astyanax altiparanae Garutti &amp;amp; Britski, 2000 (Teleostei, Characidae) from the Upper Paraná River basin, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Peres,Maria Dolores; Vasconcelos,Marcio dos Santos; Renesto,Erasmo.
Allozyme data was used to assess the genetic diversity Astyanax altiparanae populations from the floodplain of the Upper Paraná River (PR). Specimens were collected in the southern Brazilian state of Paraná from PR in Porto Rico municipality and Ribeirão Ficha (RF) in Ubiratã municipality. The authors used 15% (w/v) corn starch gel electrophoresis to identify 21 putative loci for 13 enzymatic systems: Aspartate aminotransferase, 2.6.1.1 (AAT), Acid phosphatase, 3.1.3.2 (ACP), Esterase, 3.1.1.1 (EST), Glycerol-3-phosphate dehydrogenase, 1.1.1.8 (G3PDH), Glucose-6-phosphate dehydrogenase, 1.1.1.49 (G6PDH), Glucose-6-phosphate isomerase, 5.3.1.9 (GPI), Iditol dehydrogenase, 1.1.1.14 (IDDH), Isocitrate dehydrogenase - NADP+, 1.1.1.42 (IDH), L-Lactate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allozymes; Astyanax; Fishes; Genetic variability; Paraná River floodplain.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500011

Molecular phylogeny of the genus Saguinus (Platyrrhini, Primates) based on the ND1 mitochondrial gene and implications for conservation
Provedor de dados: Genetics and Molecular Biology
Autores: Tagliaro,Claudia Helena; Schneider,Horacio; Sampaio,Iracilda; Schneider,Maria Paula Cruz; Vallinoto,Marcelo; Stanhope,Michael.
The systematics of the subfamily Callitrichinae (Platyrrhini, Primates), a group of small monkeys from South America and Panama, remains an area of considerable discussion despite many investigations, there being continuing controversy over subgeneric taxonomic classifications based on morphological characters. The purpose of our research was to help elucidate the phylogenetic relationships within the monkey genus Saguinus (Callitrichinae) using a molecular approach to discover whether or not the two different sections containing hairy-faced and bare-faced species are monophyletic, whether Saguinus midas midas and Saguinus bicolor are more closely related than are S. midas midas and Saguinus midas niger, and if Saguinus fuscicollis melanoleucus and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saguinus; Callitrichinae; ND1; Phylogeny; MtDNA; Conservation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100009

Comparative genome-wide polymorphic microsatellite markers in Antarctic penguins through next generation sequencing
Provedor de dados: Genetics and Molecular Biology
Autores: Vianna,Juliana A.; Noll,Daly; Mura-Jornet,Isidora; Valenzuela-Guerra,Paulina; González-Acuña,Daniel; Navarro,Cristell; Loyola,David E.; Dantas,Gisele P. M.
Abstract Microsatellites are valuable molecular markers for evolutionary and ecological studies. Next generation sequencing is responsible for the increasing number of microsatellites for non-model species. Penguins of the Pygoscelis genus are comprised of three species: Adélie (P. adeliae), Chinstrap (P. antarcticus) and Gentoo penguin (P. papua), all distributed around Antarctica and the sub-Antarctic. The species have been affected differently by climate change, and the use of microsatellite markers will be crucial to monitor population dynamics. We characterized a large set of genome-wide microsatellites and evaluated polymorphisms in all three species. SOLiD reads were generated from the libraries of each species, identifying a large amount of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite markers; Penguin; Genome; Polymorphism; Tetranucleotide.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400676

A comparison between SOLiD 5500XLand Ion Torrent PGM-derived miRNA expression profiles in two breast cell lines
Provedor de dados: Genetics and Molecular Biology
Autores: Branco,Gabriela Pereira; Valieris,Renan; Povoa,Lucas Venezian; Araújo,Luiza Ferreira de; Fernandes,Gustavo Ribeiro; Souza,Jorge Estefano Santana de; Amorim,Maria Galli de; Ferreira,Elisa Napolitano e; Silva,Israel Tojal da; Nunes,Diana Noronha; Dias-Neto,Emmanuel.
Abstract Next-generation sequencing (NGS) platforms allow the analysis of hundreds of millions of molecules in a single sequencing run, revolutionizing many research areas. NGS-based microRNA studies enable expression quantification in unprecedented scale without the limitations of closed-platforms. Yet, whereas a massive amount of data produced by these platforms is available, comparisons of quantification/discovery capabilities between platforms are still lacking. Here we compare two NGS-platforms: SOLiD and PGM, by evaluating their microRNA identification/quantification capabilities using two breast-derived cell-lines. A high expression correlation (R2 &gt; 0.9) was achieved, encompassing 97% of the miRNAs, and the few discrepancies in miRNA counts were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Next-generation sequencing (NGS); MiRNA expression profiles; SOLiD; PGM.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400802

Use of V H, D and J H immunoglobulin gene segments in Brazilian patients with chronic lymphocytic leukaemia (CLL)
Provedor de dados: Genetics and Molecular Biology
Autores: Pimentel,Beatriz Jatobá; Stefanoff,Cláudio Gustavo; Moreira,Aline Santos; Seuánez,Héctor N.; Zalcberg,Ilana Renault.
Chronic lymphocytic leukaemia (CLL) is a haematological malignancy for which reliable prognostic markers are needed in view of its clinical heterogeneity. In approximately 50% of CLL patients, immunoglobulin (Ig) rearrangements are modified by somatic hypermutation (SHM), a process that represents a reliable prognostic indicator of favourable progression. In this study, we investigated SHM in 37 Brazilian CLL patients and identified the preferential involvement of specific immunoglobulin gene families and segments through PCR-amplified fragments or subcloned fragments. Forty-one rearrangements were observed and 37 of them were functional. A 98% homology cut-off with germinal sequences showed 18 patients (48.7%) with SHM. Unmutated cases showed a poorer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic lymphocytic leukaemia; Immunoglobulin rearrangements; Somatic hypermutation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400007

Mesenchymal stem cell therapy promotes the improvement and recovery of renal function in a preclinical model
Provedor de dados: Genetics and Molecular Biology
Autores: Urt-Filho,Antônio; Oliveira,Rodrigo Juliano; Hermeto,Larissa Correa; Pesarini,João Renato; David,Natan de; Cantero,Wilson de Barros; Falcão,Gustavo; Marks,Guido; Antoniolli-Silva,Andréia Conceição Milan Brochado.
Abstract Acute renal failure (ARF) is an extremely important public health issue in need of novel therapies. The present study aimed to evaluate the capacity of mesenchymal stem cell (MSC) therapy to promote the improvement and recovery of renal function in a preclinical model. Wistar rats were used as the experimental model, and our results show that cisplatin (5mg/kg) can efficiently induce ARF, as measured by changes in biochemical (urea and creatinine) and histological parameters. MSC therapy performed 24h after the administration of chemotherapy resulted in normalized plasma urea and creatinine levels 30 and 45d after the onset of kidney disease. Furthermore, MSC therapy significantly reduced histological changes (intratubular cast formation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow; Chemotherapy; Nephrotoxicity; Kidney disease; Tissue regeneration.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200290

Protocol for extraction of genomic DNA from swine solid tissues
Provedor de dados: Genetics and Molecular Biology
Autores: Biase,Fernando Henrique; Franco,Maurício Machaim; Goulart,Luiz Ricardo; Antunes,Robson Carlos.
Molecular diagnostics are performed by using DNA from different body tissues. However, it is necessary to obtain genomic DNA of good quality. Due to the impossibility of collecting blood from slaughtered animals, DNA extraction from solid tissues is necessary. The objective of this study was to describe a protocol of DNA extraction from swine skin, adipose, brain, liver, kidney and muscle tissues. We obtained high molecular weight DNA of good quality, shown by agarose gel and amplification of two DNA fragments, 605bp and 891pb, by PCR. Spectrophotometric analysis of DNA concentration showed variation among the DNA from different tissues, with the liver and adipose tissues presenting the greatest and the smallest concentration, respectively. The described...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: DNA extraction; Swine tissues.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300011

Analysis of the synaptonemal complex of the nine-banded armadillo, Dasypus novemcinctus
Provedor de dados: Genetics and Molecular Biology
Autores: Scavone,Márcia Denise da Paixão; Oliveira,Claudio; Bagagli,Eduardo; Foresti,Fausto.
The synaptonemal complex (SC) of three specimens of the nine-banded armadillo (Dasypus novemcinctus) was analyzed. Thirty-two bivalents (2n = 64) were observed, 31 of them being autosomes and one an XY sexual bivalent. Chromosome synapsis processes and nucleolus structure changes were analyzed in zygotene and pachytene cells, allowing a detailed description of the beginning of meiotic prophase in this species. There was complete synapsis of X and Y chromosomes. Some abnormalities in SC were observed in cells during zygotene and at the beginning of pachytene, but not in cells in the middle and late pachytene, suggesting the occurrence of synaptic adjustments in their SC.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300020

CTLA-4 gene polymorphisms are associated with obesity in Turner Syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Luana Oliveira dos; Bispo,Adriana Valéria Sales; Barros,Juliana Vieira de; Laranjeira,Raysa Samanta Moraes; Pinto,Rafaella do Nascimento; Silva,Jaqueline de Azevêdo; Duarte,Andréa de Rezende; Araújo,Jacqueline; Sandrin-Garcia,Paula; Crovella,Sergio; Bezerra,Marcos André Cavalcanti; Belmont,Taciana Furtado de Mendonça; Cavalcanti,Maria do Socorro; Santos,Neide.
Abstract Turner syndrome (TS) is characterized by a set of clinical conditions, including autoimmune/inflammatory diseases and infectious conditions, that can compromise a patient’s quality of life. Here we assessed polymorphisms in CTLA-4 +49A/G (rs231775), PTPN22 +1858G/A (rs2476601), and MBL2 -550 (H/L) (rs11003125), -221(X/Y) (rs7096206) and exon 1 (A/O) in women from northeastern Brazil to determine whether polymorphisms within these key immune response genes confer differential susceptibility to clinical conditions in TS. A case-control genetic association study was performed, including 86 female TS patients and 179 healthy women. An association was observed for the A/G genotype of CTLA-4 +49A/G in TS patients (p=0.043, odds ratio [OR]=0.54). In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CTLA-4 gene; Immune genes; Obesity; Polymorphism; Turner syndrome.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500727

A leukocyte cryopreservation technique for cytogenetic studies
Provedor de dados: Genetics and Molecular Biology
Autores: Duarte,José Maurício Barbanti; Ramalho,Mariney Flávia Pereira Di Tano; Lima,Vera Fernanda Hossepian de; Jorge,Wilham.
Several culture, cryopreservation, freezing and thawing methods were tested to develop an efficient technique for storing cervid and ovine leukocytes. The best results were obtained with McCoy or Vega y Martinez (VYM) solution with dimethyl sulfoxide (DMSO), horse serum and polyvinylpyrrolidone (PVP) as cryopreservatives. The best protocol for freezing was 4°C for 30 min followed by 15 min in liquid nitrogen vapor. To thaw, the still frozen material was placed onto cultivation medium for propagation.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300019

Alexandre Afranio Peixoto (1963-2013)
Provedor de dados: Genetics and Molecular Biology
Autores: Klaczko,Louis Bernard; Bruno,Rafaela V.
Tipo: Info:eu-repo/semantics/other
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200001

Inheritance of a long juvenile period under short-day conditions in soybean
Provedor de dados: Genetics and Molecular Biology
Autores: Carpentieri-Pípolo,Valéria; Almeida,Leones Alves de; Kiihl,Romeu Afonso de S.
The long juvenile period (LJP) characteristic of soybean (Glycine max L. Merrill) cultivars delays flowering under short-day conditions. This trait may be important in increasing the adaptation range of soybean to low latitudes and provides greater flexibility for sowing times within the same latitude. The inheritance of the long juvenile period was studied in the MG/BR22 (Garimpo) soybean cultivar to provide knowledge to support the development of cultivars adapted to short day conditions. Four cultivars (‘Paraná’, ‘Bossier’, ‘Bragg’, and ‘Davis’) were crossed among each other and with MG/BR 22 (‘Garimpo’). The study was conducted under short-day conditions (early sowing) in a greenhouse and in the field, in Londrina, Paraná, (23°22’ south latitude). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Long juvenile period; Soybean; Glycine max; Delayed flowering; Short-days.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400016

Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelensis (Characiformes, Characidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Nirchio,Mauro; Oliveira,Claudio; Ferreira,Irani Alves; Granado,Angel; Ron,Ernesto.
The karyotype and chromosomal characteristics of the characid fish Triportheus venezuelensis were investigated using differential staining techniques (C-banding, Ag-NOR staining) and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. The diploid chromosome number (2n = 52), karyotype composition and sex chromosome determination system of the ZZ/ZW type were the same as previously described in other species of the genus Triportheus. However, extensive variation regarding nucleolus organizer regions (NOR) different from other species was observed. 18S rDNA sequences were distributed on nine chromosome pairs, but the number of chromosomes with Ag-NORs was usually lower, reaching a maximum of four chromosomes. When sequential staining experiments...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 18S rDNA; Ag-NORs; C-band; FISH; Fish cytogenetics; Sex chromosomes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100007

Mating system in a natural population of Theobroma grandiflorum (Willd. ex Spreng.) Schum., by microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Alves,Rafael M.; Artero,Angela S.; Sebbenn,Alexandre M.; Figueira,Antonio.
The aim of this research was to study the mating system of a natural population of Theobroma grandiflorum (cupuassu) from Nova Ipixuna, Pará state, using microsatellite markers. Eight polymorphic microsatellite loci were analyzed in eight families, each represented by 10 six-month old seedlings derived from open-pollinated pods. The estimation for the multilocus outcrossing rate (&lt;img border=0 id="_x0000_i1026" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle&gt;m = 1.0) and individual outcrossing rate (&lt;img border=0 id="_x0000_i1027" src="../../img/revistas/gmb/v26n3/a25img01.gif" align=absmiddle&gt; = 1.0) for this population suggests that T. grandiflorum may be a perfect outbreeding (allogamous) species. Likewise, for the studied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazonia; Germplasm; Cupuassu; Genetic conservation; Simple sequence repeat.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300025

Expression of D-type cyclins in differentiating cells of the mouse spinal cord
Provedor de dados: Genetics and Molecular Biology
Autores: Salles,Patricy de Andrade; Fortes,Juliana Chagas; Guedes,Maria Izabel Florindo; Weller,Mathias.
The D-type cyclins form complexes with the cyclin dependent (CD) kinases CDK4 and CDK6 and promote the G1-S phase transition of the cell cycle by antagonizing the retinoblastoma suppresser protein pRB. In the developing nervous system D-type cyclins show spatially and temporally dynamic patterns of expression. We demonstrated that cyclin D1 was transiently expressed in differentiating spinal cord ventral interneurons while cyclin D3 protein was expressed in differentiating motor neurons and dorsal interneurons. This expression of cyclin D3 in neurons of the mantle zone was extended to all regions of the spinal cord at E15.5. The results suggest that cyclin D1 and D3 have specific functions in differentiating neurons. Similarly, in the developing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell cycle; D-type cyclins; Midbrain-hindbrain region; Neurogenesis; Spinal cord.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400032

Linkage disequilibrium, SNP frequency change due to selection, and association mapping in popcorn chromosome regions containing QTLs for quality traits
Provedor de dados: Genetics and Molecular Biology
Autores: Paes,Geísa Pinheiro; Viana,José Marcelo Soriano; Silva,Fabyano Fonseca e; Mundim,Gabriel Borges.
Abstract The objectives of this study were to assess linkage disequilibrium (LD) and selection-induced changes in single nucleotide polymorphism (SNP) frequency, and to perform association mapping in popcorn chromosome regions containing quantitative trait loci (QTLs) for quality traits. Seven tropical and two temperate popcorn populations were genotyped for 96 SNPs chosen in chromosome regions containing QTLs for quality traits. The populations were phenotyped for expansion volume, 100-kernel weight, kernel sphericity, and kernel density. The LD statistics were the difference between the observed and expected haplotype frequencies (D), the proportion of D relative to the expected maximum value in the population, and the square of the correlation between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gametic phase disequilibrium; GWAS; Candidate gene.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100097

Vegetable and fruit juice enhances antioxidant capacity and regulates antioxidant gene expression in rat liver, brain and colon
Provedor de dados: Genetics and Molecular Biology
Autores: Yuan,Linhong; Liu,Jinmeng; Zhen,Jie; Xu,Yao; Chen,Shuying; Halm-Lutterodt,Nicholas Van; Xiao,Rong.
Abstract To explore the effect of fruit and vegetable (FV) juice on biomarkers of oxidative damage and antioxidant gene expression in rats, 36 adult male Wistar rats were randomly divided into control, low FV juice dosage or high FV juice dosage treatment groups. The rats were given freshly extracted FV juice or the same volume of saline water daily for five weeks. After intervention, serum and tissues specimens were collected for biomarker and gene expression measurement. FV juice intervention increased total antioxidant capacity, glutathione, vitamin C, β-carotene, total polyphenols, flavonoids levels andglutathione peroxidaseenzyme activity in rat serum or tissues (p &lt; 0.05). FV juice intervention caused reduction of malondialdehyde levels in rat...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Biomarkers; Fruit and vegetable; Gene expression.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100134

A growth hormone-based phylogenetic analysis of euteleostean fishes including a representative species of the Atheriniformes Order, Odontesthes argentinensis
Provedor de dados: Genetics and Molecular Biology
Autores: Marins,Luis F.; Levy,Jose A.; Folch,Josep M.; Sanchez,Armand.
The GH (growth hormone) cDNA sequence of the marine silverside fish Odontesthes argentinensis was obtained using the RACE protocol (Rapid Amplification of cDNA Ends). The marine silverside GH cDNA sequence is 928 nucleotides long and was found to encode a polypeptide of 204 amino acids, including a signal peptide of 17 amino acids. The 5' and 3' untranslated regions of the messenger are 109 and 204 nucleotides long, respectively. The deduced GH amino acid sequence was used to infer a phylogenetic tree with GH amino acid sequences from representative species belonging to the Euteleostei Subdivision using the maximum parsimony method. The topology found is according to the major phylogenetic grouping of euteleosts. The results corroborate the hypothesis that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth hormone; CDNA; RACE (Rapid Amplification of cDNA Ends); Phylogeny.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300013

Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)
Provedor de dados: Genetics and Molecular Biology
Autores: Novelli,Valdenice M.; Cristofani,Mariangela; Souza,Alessandra A.; Machado,Marcos A.
The aims of this study were to develop simple sequence repeat markers (SSRs or microsatellite markers) in citrus and to evaluate the efficiency of these markers for characterization of sweet orange. We developed SSRs from a genomic library of 'Pêra IAC' sweet orange enriched for AG/TC, GT/CA, TCA/AGT and AAC/TTG sequence repeats. We selected 279 sequences from which 171 primer pairs were designed of which 113 with the best banding patterns were selected. Characterization of sweet orange microsatellite loci revealed that AG/TC was the most abundant (69%) microsatellite class isolated, followed by GT/CA (15.9%), TCA/AGT (8%) and AAC/TTG (6.2%). The number of alleles ranged from 1 to 4, with a mean of 2 alleles per locus. Four microsatellite loci developed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus species; Germplasm bank; Polymorphism; Molecular markers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100018

Chromosomal evidence of population subdivision in the freshwater fish Leporinus elongatus in the Upper Paraná River basin
Provedor de dados: Genetics and Molecular Biology
Autores: Molina,Wagner Franco; Shibatta,Oscar; Galetti Jr.,Pedro Manoel.
Cytogenetic analyses performed in populations of the Neotropical freshwater fish Leporinus elongatus of the Upper Paraná River basin showed a chromosome polymorphism involving heterochromatic segments of the nucleolar organizing pair in this species. The NOR-bearing chromosome pair was characterized by two phenotypes identified by the absence (C¹) or presence (C²) of a heterochromatic segment located in an interstitial position of the long arm in this pair. The meiotic segregation of these variations results in three distinct cytotypes, C¹C¹, C¹C² and C²C². Both populations were in Hardy-Weinberg equilibrium. Sex-related cytotype divergence was identified and multivariate analyses revealed that the C²C² individuals presented morphometric differentiation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characiformes; Anostomidae; Heterochromatin; Chromosome polymorphism.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200020

Use of seed protein polymorphism for discrimination of improvement level and geographic origin of upland rice cultivars
Provedor de dados: Genetics and Molecular Biology
Autores: Montalván,Ricardo; Ando,Akihiko; Echeverrigaray,Sérgio.
Grain proteins from 58 Brazilian and nine Japanese upland rice cultivars (Oryza sativa L.) were electrophoretically separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Densitometric scanning of the electrophoretic profiles permitted the estimation of the relative concentration of 16 protein fractions, which were used as variables for the calculation of Fisher's canonical discriminating functions. Significant differences between mean values of protein fractions were useful in distinguishing Brazilian and Japanese cultivars, as well as improved and non-improved Brazilian rice cultivars in scattered plots. Electrophoretically detectable protein polymorphism in rice grain can indicate geographic origin as well as breeding...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400021

Cytogenetics and embryology of Eupatorium laevigatum (Compositae)
Provedor de dados: Genetics and Molecular Biology
Autores: Bertasso-Borges,Maristela Sanches; Coleman,James Robert.
Embryological studies indicate Eupatorium laevigatum to have Antennaria type diplospory with precocious embryony. The embryo sac is of the Polygonum type and the polar nuclei fuse before anthesis (maturation of the stamens). Endosperm development is autonomous and the central cell divides only after the initial stages of embryo formation. It is estimated that about 10% of the florets in anthesis contain an undivided egg which can be used for sexual reproduction. The study of microsporogenesis revealed abnormalities in chromosome pairing which result in the formation of univalents, bivalents, trivalents and higher polyvalents, with the consequent production of lagging chromosomes, unbalanced nuclei, micronuclei and sterile pollen. We found that, as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eupatorium; Apomixis; Plant cytogenetics; Plant embryology; Asteraceae.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100022

Electroacupuncture inhibits apoptosis in annulus fibrosis cells through suppression of the mitochondria-dependent pathway in a rat model of cervical intervertebral disc degradation
Provedor de dados: Genetics and Molecular Biology
Autores: Liao,Jun; Ke,Meigui; Xu,Teng; Lin,Lili.
The purpose of this study was to investigate whether treatment with electroacupuncture (EA) inhibited mitochondria-dependent apoptosis in annulus fibrosis (AF) cells in a rat model of cervical intervertebral disc degradation induced by unbalanced dynamic and static forces. Forty Sprague-Dawley rats were used in this study, of which 30 underwent surgery to induce cervical intervertebral disc degradation, 10 rats received EA at acupoints Dazhui (DU 14) and Shousanli (LI 10). TUNEL staining was measured to assess apoptosis in AF cells, immunohistochemistry was used to examine Bcl-2 and Bax expression, colorimetric assays were used to determine caspase 9 and caspase 3 activities and RT-PCR and western blotting were used to assess the mRNA and protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Annulus fibrosis cells; Apoptosis; Electroacupuncture; Mitochondria.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400021

Association of XRCC1 Arg399GIn and Tp53 Arg72Pro polymorphisms and increased risk of uterine leiomyoma - A case-control study
Provedor de dados: Genetics and Molecular Biology
Autores: Yaghmaei,Minoo; Salimi,Saeedeh; Namazi,Lida; Farajian-Mashhadi,Farzaneh.
Abstract The aim of present study was to investigate the role of the X-ray repair cross-complementing protein1 (XRCC1) and Tumor protein p53 (Tp53) polymorphisms in Uterine Leiomyoma (UL) susceptibility in southeastern Iran. This case control study was performed on 139 women with UL and 149 age, BMI and ethnicity matched healthy women. All women were genotyped for the XRCC1 Arg399Gln, XRCC1 Arg194Trp and Tp53 Arg72Pro polymorphisms. The frequency of Tp53 72 Pro/Pro genotype was significantly higher in UL women compared to controls. The risk of UL was 1.5 fold higher in women with the Pro/Pro genotype (OR, 1.5 [95% CI, 1.1 to 2.1], p = 0.012). Moreover, the frequency of the Pro allele was significantly higher in the UL women. Although the frequency of XRCC1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uterine leiomyoma; Tp53; XRCC1; Polymorphism.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400444

Isolation and characterization of a promoter responsive to salt, osmotic and dehydration stresses in soybean
Provedor de dados: Genetics and Molecular Biology
Autores: Conforte,Alessandra Jordano; Guimarães-Dias,Fábia; Neves-Borges,Anna Cristina; Bencke-Malato,Marta; Felix-Whipps,Durvalina; Alves-Ferreira,Márcio.
Abstract Drought stress is the main limiting factor of soybean yield. Currently, genetic engineering has been one important tool in the development of drought-tolerant cultivars. A widely used strategy is the fusion of genes that confer tolerance under the control of the CaMV35S constitutive promoter; however, stress-responsive promoters would constitute the best alternative to the generation of drought-tolerant crops. We characterized the promoter of α-galactosidase soybean (GlymaGAL) gene that was previously identified as highly up-regulated by drought stress. The β-glucuronidase (GUS) activity of Arabidopsis transgenic plants bearing 1000- and 2000-bp fragments of the GlymaGAL promoter fused to the uidA gene was evaluated under air-dried, polyethylene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-galactosidase gene; Salt stress; Drought-tolerant soybean; Cis-acting element; Raffinose degradation pathway.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200226

Development of a comprehensive noninvasive prenatal test
Provedor de dados: Genetics and Molecular Biology
Autores: Malcher,Carolina; Yamamoto,Guilherme L.; Burnham,Philip; Ezquina,Suzana A.M.; Lourenço,Naila C.V.; Balkassmi,Sahilla; Antonio,David S. Marco; Hsia,Gabriella S.P.; Gollop,Thomaz; Pavanello,Rita C.; Lopes,Marco Antonio; Bakker,Egbert; Zatz,Mayana; Bertola,Débora; Vlaminck,Iwijn De; Passos-Bueno,Maria Rita.
Abstract Our aim was to develop and apply a comprehensive noninvasive prenatal test (NIPT) by using high-coverage targeted next-generation sequencing to estimate fetal fraction, determine fetal sex, and detect trisomy and monogenic disease without parental genotype information. We analyzed 45 pregnancies, 40 mock samples, and eight mother-child pairs to generate 35 simulated datasets. Fetal fraction (FF) was estimated based on analysis of the single nucleotide polymorphism (SNP) allele fraction distribution. A Z-score was calculated for trisomy of chromosome 21 (T21), and fetal sex detection. Monogenic disease detection was performed through variant analysis. Model validation was performed using the simulated datasets. The novel model to estimate FF was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell-free DNA; Next-generation sequencing; Trisomy; Noninvasive prenatal test; Fetal fraction.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400545

Isolation and sequencing of seven Sox genes from the lacertid lizard Eremias breuchleyi
Provedor de dados: Genetics and Molecular Biology
Autores: Jie,Li; Zheng,Ping-Ping; Song,Jiao-Lian; Rui,Jin-Long; Nie,Liu-Wang.
The Sox family of genes shares a high sequence similarity with the HMG box region of the human Y chromosomal gene, SRY. We used highly degenerate primers to clone and sequence seven Eremias breuchleyi Sox genes (EbSox2, EbSox3, EbSox4, EbSox11, EbSox12, EbSox14 and EbSox21). A database search for the cloned sequences revealed the following percentage identity with the homologous human SOX genes: EbSox2 = 96%, EbSox3 = 88%, EbSox4 = 94%, EbSox11 = 99%, EbSox12 = 96%, EbSox14 = 98%, EbSox21 = 97%. Cluster analysis indicates that they seem to belong to group B and group C of Sox gene family, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eremias breuchleyi; PCR; Sequence analysis; Sox genes; SSCP.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300031

Mitochondrial DNA single nucleotide polymorphism associated with weight estimated breeding values in Nelore cattle (Bos indicus)
Provedor de dados: Genetics and Molecular Biology
Autores: Biase,Fernando Henrique; Meirelles,Flávio Vieira; Gunski,Ricardo; Vozzi,Pedro Alejandro; Bezerra,Luiz A.F.; Vila,Reginaldo A.; Rosa,Artur J.M.; Lôbo,Raysildo B.; Martelli,Lúcia.
We sampled 119 Nelore cattle (Bos indicus), 69 harboring B. indicus mtDNA plus 50 carrying Bos taurus mtDNA, to estimate the frequencies of putative mtDNA single nucleotide polymorphisms (SNPs) and investigate their association with Nelore weight and scrotal circumference estimated breeding values (EBVs). The PCR restriction fragment length polymorphism (PCR-RFLP) method was used to detect polymorphisms in the mitochondrial asparagine, cysteine, glycine, leucine and proline transporter RNA (tRNA) genes (tRNAasn, tRNAcys, tRNAgly, tRNAleu and tRNApro). The 50 cattle carrying B. taurus mtDNA were monomorphic for all the tRNA gene SNPs analyzed, suggesting that they are specific to mtDNA from B. indicus cattle. No tRNAcys or tRNAgly polymorphisms were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Mitochondria; MtDNA; SNP; Weight.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600005

Between the cross and the sword: the crisis of the gene concept
Provedor de dados: Genetics and Molecular Biology
Autores: El-Hani,Charbel Niño.
Challenges to the gene concept have shown the difficulty of preserving the classical molecular concept, according to which a gene is a stretch of DNA encoding a functional product (polypeptide or RNA). The main difficulties are related to the overlaying of the Mendelian idea of the gene as a ‘unit’: the interpretation of genes as structural and/or functional units in the genome is challenged by evidence showing the complexity and diversity of genomic organization. This paper discusses the difficulties faced by the classical molecular concept and addresses alternatives to it. Among the alternatives, it considers distinctions between different gene concepts, such as that between the ‘molecular’ and the ‘evolutionary’ gene, or between ‘gene-P’ (the gene as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene; Classical molecular gene concept; Mendelian gene; Genomic complexity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300001

Clinical checklists in the selection of mentally retarded males for molecular screening of fragile X syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Christofolini,Denise M.; Lipay,Monica V.N.; Ramos,Marco Antonio P.; Costa,Silvia S.; Bellucco,Fernanda T.S.; Nogueira,Sintia I.; Kulikowski,Leslie D.; Brunoni,Décio; Melaragno,Maria Isabel.
Fragile X syndrome is the most frequent cause of inherited mental retardation. The phenotype in this syndrome is quite variable and less conspicuous in younger patients, making clinical diagnosis difficult and thus making molecular diagnosis necessary. The use of clinical checklists in mentally retarded individuals can help selecting patients to be given priority in the molecular investigation for the fragile-X mutation in the FMR1 gene. We evaluated two clinical checklists in a sample of 200 Brazilian male patients with mental retardation. The highest scores in the two checklists concentrated among the 19 males (9.5%) found to carry full mutations. Our results confirm the importance of fragile-X checklists as a clinical tool in the study of mentally...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FMR1 gene; Fragile X syndrome checklist; Molecular methods; X-linked mental retardation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600002

BOX-PCR-based identification of bacterial species belonging to Pseudomonas syringae: P. viridiflava group
Provedor de dados: Genetics and Molecular Biology
Autores: Marques,Abi S.A.; Marchaison,Anne; Gardan,Louis; Samson,Régine.
The phenotypic characteristics and genetic fingerprints of a collection of 120 bacterial strains, belonging to Pseudomonas syringae sensu lato group, P. viridiflava and reference bacteria were evaluated, with the aim of species identification. The numerical analysis of 119 nutritional characteristics did not show patterns that would help with identification. Regarding the genetic fingerprinting, the results of the present study supported the observation that BOX-PCR seems to be able to identify bacterial strains at species level. After numerical analyses of the bar-codes, all pathovars belonging to each one of the nine described genomospecies were clustered together at a distance of 0.72, and could be separated at genomic species level. Two P. syringae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas syringae; Phenotypic characters; Genomospecies; BOX-PCR; Bacterial identification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100019

Syntenic homology of human unique DNA sequences within chromossome regions 5q31, 10q22, 13q32-33 and 19q13.1 in the great apes
Provedor de dados: Genetics and Molecular Biology
Autores: Vallente-Samonte,Rhea U.; Conte,Robert A.; Verma,Ram S.
Homologies between chromosome banding patterns and DNA sequences in the great apes and humans suggest an apparent common origin for these two lineages. The availability of DNA probes for specific regions of human chromosomes (5q31, 10q22, 13q32-33 and 19q13.1) led us to cross-hybridize these to chimpanzee (Pan troglodytes, PTR), gorilla (Gorilla gorilla, GGO) and orangutan (Pongo pygmaeus, PPY) chromosomes in a search for equivalent regions in the great apes. Positive hybridization signals to the chromosome 5q31-specific DNA probe were observed at HSA 5q31, PTR 4q31, GGO 4q31 and PPY 4q31, while fluorescent signals using the chromosome 10q22-specific DNA probe were noted at HSA 10q22, PTR 8q22, GGO 8q22 and PPY 7q22. The chromosome arms showing...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300004

Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants
Provedor de dados: Genetics and Molecular Biology
Autores: Falco,J.R.P.; Mello,M.L.S.
The critical electrolyte concentrations (CEC) of sperm chromatin from animal species known or suspected to contain histone H1 variants were compared by examining the affinity of their DNA-protein complexes for toluidine blue in the presence of Mg2+. Bullfrog, sea urchin, bee and bumblebee spermatozoa were studied. The CEC for Rana catesbeiana and two sea urchin species were similar to that of histone H5-containing chromatin from chicken erythrocytes, thus confirming the biochemical and structural similarities of these DNA-protein complexes. The CEC for bees and the bumblebee, Bombus atratus, showed no particular phylogenetic relationship. We concluded that the CEC of histone H1-containing sperm cell chromatin is a useful indicator of variability in...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200010

Estimation of variance components and prediction of breeding values in rubber tree breeding using the REML/BLUP procedure
Provedor de dados: Genetics and Molecular Biology
Autores: Furlani,Renata Capistrano Moreira; Moraes,Mario Luiz Teixeira de; Resende,Marcos Deon Vilela de; Furlani Junior,Enes; Gonçalves,Paulo de Souza; Valério Filho,Walter Veriano; Paiva,João Rodrigues de.
The present paper deals with estimation of variance components, prediction of breeding values and selection in a population of rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Müell.-Arg.] from Rio Branco, State of Acre, Brazil. The REML/BLUP (restricted maximum likelihood/best linear unbiased prediction) procedure was applied. For this purpose, 37 rubber tree families were obtained and assessed in a randomized complete block design, with three unbalanced replications. The field trial was carried out at the Experimental Station of UNESP, located in Selvíria, State of Mato Grosso do Sul, Brazil. The quantitative traits evaluated were: girth (G), bark thickness (BT), number of latex vessel rings (NR), and plant height (PH). Given the unbalanced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Linear mixed model; Effective population size; Breeding strategies; Rubber tree improvement.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200017

Phylogenetic autocorrelation and evolutionary diversity of Carnivora (Mammalia) in Conservation Units of the New World
Provedor de dados: Genetics and Molecular Biology
Autores: Tôrres,Natália Mundim; Diniz-Filho,José Alexandre Felizola.
One of the main concerns of Conservation Biology is the identification of priority areas for conservation, and the development of quantitative methods is important to achieve this task. Many phylogenetic diversity indexes and higher-taxon approaches have been used in this context. In this study, Faith's phylogenetic indexes and the number of evolutionary independent lineages of Carnivora were calculated at the average patch level based on phylogenetic autocorrelation analysis of phenotypic traits, in 18 conservation units in America (frequently National Parks). Despite controversies about the hierarchical level to be adopted, the characters included in this study suggest that the family level produces independent units for the analysis of phenotypic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogenetic autocorrelation; Carnivora; Conservation units; New World.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400008

Evolutionary history of  Phakopsora pachyrhizi  (the Asian soybean rust) in Brazil based on nucleotide sequences of the internal transcribed spacer region of the nuclear ribosomal DNA
Provedor de dados: Genetics and Molecular Biology
Autores: Freire,Maíra C. M.; Oliveira,Luiz O. de; Almeida,Álvaro M. R. de; Schuster,Ivan; Moreira,Maurilio A.; Liebenberg,Merion M.; Mienie,Charlotte M. S.
Phakopsora pachyrhizi  has dispersed globally and brought severe economic losses to soybean growers. The fungus has been established in Brazil since 2002 and is found nationwide. To gather information on the temporal and spatial patterns of genetic variation in  P. pachyrhizi , we sequenced the nuclear internal transcribed spacer regions (ITS1 and ITS2). Total genomic DNA was extracted using either lyophilized urediniospores or lesions removed from infected leaves sampled from 26 soybean fields in Brazil and one field in South Africa. Cloning prior to sequencing was necessary because direct sequencing of PCR amplicons gave partially unreadable electrophoretograms with peak displacements suggestive of multiple sequences with length polymorphism. Sequences...
Palavras-chave: Phakopsora pachyrhizi; Diversity; Internal transcribed spacer; Asian soybean rust; Phylogeography.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500020

Nine karyomorphs for spiny rats of the genus Proechimys (Echimyidae, Rodentia) from North and Central Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Machado,Taís; Silva,Maria José de J.; Leal-Mesquita,Emygdia Rosa; Carmignotto,Ana Paula; Yonenaga-Yassuda,Yatiyo.
Spiny rats of the genus Proechimys are morphologically diverse, widely distributed and have diploid numbers ranging from 2n = 14-16 to 2n = 62. In this paper we present cytogenetical data and brief comments on morphological and biogeographical issues related to spiny rats. In our sample of 42 spiny rats collected from 12 Brazilian Amazonian tropical rainforest and the Cerrado (Brazilian savanna) sites we detected nine karyological entities: four different karyomorphs with 2n = 30, three with 2n = 28, one with 2n = 15 and one with 2n = 44. Based on qualitative morphological characters these karyomorphs can be allocated to five species within the goeldii, guyannensis and longicaudatus species groups.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proechimys; Rodents; Cytogenetics; Karyomorph; Morphology.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500007

SSR and AFLP based genetic diversity of soybean germplasm differing in photoperiod sensitivity
Provedor de dados: Genetics and Molecular Biology
Autores: Singh,Ram K.; Bhatia,Virendra S.; Bhat,K.V.; Mohapatra,Trilochan; Singh,Nagendra K.; Bansal,Kailash C.; Koundal,K.R.
Forty-four soybean genotypes with different photoperiod response were selected after screening of 1000 soybean accessions under artificial condition and were profiled using 40 SSR and 5 AFLP primer pairs. The average polymorphism information content (PIC) for SSR and AFLP marker systems was 0.507 and 0.120, respectively. Clustering of genotypes was done using UPGMA method for SSR and AFLP and correlation was 0.337 and 0.504, respectively. Mantel's correlation coefficients between Jaccard's similarity coefficient and the cophenetic values were fairly high in both the marker systems (SSR = 0.924; AFLP = 0.958) indicating very good fit for the clustering pattern. UPGMA based cluster analysis classified soybean genotypes into four major groups with fairly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photoperiod response; SSR; AFLP; Genetic diversity; Soybean.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200019

Genetic variation in body weight gain and composition in the intercross of Large (LG/J) and Small (SM/J) inbred strains of mice
Provedor de dados: Genetics and Molecular Biology
Autores: Kramer,Melissa G.; Vaughn,Ty T.; Pletscher,L. Susan; King-Ellison,Kelly; Adams,Emily; Erikson,Christopher; Cheverud,James M.
Strain intercross experiments provide a powerful means for mapping genes affecting complex quantitative traits. We report on the genetic variability of the intercross of the Large (LG/J) and Small (SM/J) inbred mouse strains as a guide to gene mapping studies. Ten SM/J males were crossed to 10 LG/J females, after which animals were randomly mated to produce F1, F2, and F3 intercross generations. The 1632 F3 animals from 200 full-sib families were used to estimate heritabilities and genetic correlations of the traits measured. A subset of families was cross-fostered at birth to allow measurement of the importance of post-natal maternal effects. Data was collected on weekly body weight from one to 10 weeks and on organ weights, body weight, reproductive fat...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200007

Novel mutation in ENG gene causing Hereditary Hemorrhagic Telangiectasia in a Peruvian family
Provedor de dados: Genetics and Molecular Biology
Autores: Zevallos-Morales,Alejandro; Murillo,Alexis; Dueñas-Roque,Milagros M.; Prötzel,Ana; Venegas-Tresierra,Luis; Ángeles-Villalba,Verónica; Guevara-Cruz,Miguel; Chávez-Gil,Ada; Fujita,Ricardo; Guevara-Fujita,Maria L.
Abstract Hereditary Hemorrhagic Telangiectasia (HHT) is a rare disorder of vascular development. Common manifestations include epistaxis, telangiectasias and arteriovenous malformations (AVMs) in multiple organs. Most patients have deletions or missense mutations in the ENG or ACVRL1 gene respectively, significantly affecting endothelium homeostasis. We analyzed the ENG gene in five members of a Peruvian family affected by HHT. One novel mutation was found in exon four of the ENG gene c.408delA, at aminoacid residue 136. This mutation changes the subsequent reading frame producing an early stop at residue 162, preserving only one fourth of the normal protein of 658 aa. This mutation was found in the four affected members of family.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: ENG; Hereditary Hemorrhagic Telangiectasia; Osler-Weber-Rendu disease.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100109

Varroa jacobsoni infestation of adult Africanized and Italian honey bees (Apis mellifera) in mixed colonies in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Moretto,Geraldo; Mello Jr.,Leonidas João de.
Different levels of infestation with the mite Varroa jacobsoni have been observed in the various Apis mellifera races. In general, bees of European races are more susceptible to the mite than African honey bees and their hybrids. In Brazil honey bee colonies are not treated against the mite, though apparently both climate and bee race influence the mite infestation. Six mixed colonies were made with Italian and Africanized honey bees. The percentage infestation by this parasite was found to be significantly lower in adult Africanized (1.69 ± 0.44) than Italian bees (2.79 ± 0.65). This ratio was similar to that found in Mexico, even though the Africanized bees tested there had not been in contact with varroa, compared to more than 20 years of the...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300006

Genetic and phenotypic parameters of productivity traits on the first three lactations in Gyr cattle herds
Provedor de dados: Genetics and Molecular Biology
Autores: Albuquerque,Maria do Socorro Maués; Freitas,Maria Armênia Ramalho de; Teodoro,Roberto Luiz.
Records of Gyr cows selected for milk production were obtained from the National Gyr Dairy Cattle Breeding Program (Embrapa/CNPGL) and analyzed, in order to estimate genetic parameters for the first three lactations and to verify the effects of some environmental factors on milk production from 1979 to 1994. Genetic parameters were estimated by REML with an animal model and a group of fixed effects that included classes of herd, year, season and age at calving. Milk production means and standard deviations were 2,183 kg, 707 kg; 2,682 kg, 762 kg and 2,638 kg, 851 kg, for first, second, and third lactations, respectively. Heritability estimates were 0.20, 0.12, and 0.19 for first, second, and third lactations, respectively, and repeatability was 0.44....
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200007

Polymorphisms of cytochrome P450 1A1, glutathione s-transferases M1 and T1 genes in ouangolodougou (Northern Ivory Coast)
Provedor de dados: Genetics and Molecular Biology
Autores: Santovito,Alfredo; Burgarello,Claudio; Cervella,Piero; Delpero,Massimiliano.
In this study, the frequencies of CYP1A1, GSTM1, and GSTT1 gene polymorphisms were determined in 133 healthy individuals from Ouangolodougou, a small rural town situated in the north of the Ivory Coast. As appeared in several published studies, ethnic differences in these frequencies have been found to play an important role in the metabolism of a relevant number of human carcinogens. In the studied sample, the frequencies of Ile/Ile (wild type), Ile/Val (heterozygous variant), and Val/Val (homozygous variant) CYP1A1 genotypes were 0.271, 0.692, and 0.037, respectively. Frequencies of GSTM1 and GSTT1 null genotypes were 0.361 and 0.331, respectively. No significant differences were noted between men and women. In contrast to published data for Africans,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CYP1A1; GSTM1; GSTT1; Genetic polymorphism; Ouangolodougou.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300006

The effects of old and recent migration waves in the distribution of HBB*S globin gene haplotypes
Provedor de dados: Genetics and Molecular Biology
Autores: Lindenau,Juliana D.; Wagner,Sandrine C.; Castro,Simone M. de; Hutz,Mara H.
Abstract Sickle cell hemoglobin is the result of a mutation at the sixth amino acid position of the beta (β) globin chain. The HBB*S gene is in linkage disequilibrium with five main haplotypes in the β-globin-like gene cluster named according to their ethnic and geographic origins: Bantu (CAR), Benin (BEN), Senegal (SEN), Cameroon (CAM) and Arabian-Indian (ARAB). These haplotypes demonstrated that the sickle cell mutation arose independently at least five times in human history. The distribution of βS haplotypes among Brazilian populations showed a predominance of the CAR haplotype. American populations were clustered in two groups defined by CAR or BEN haplotype frequencies. This scenario is compatible with historical records about the slave trade in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ΒS globin haplotypes; Sickle cell disease; Hemoglobin S; Migration.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400515

A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
Provedor de dados: Genetics and Molecular Biology
Autores: Deprá,Maríndia; Sepel,Lenira Maria Nunes; Loreto,Élgion Lucio da Silva.
We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenic Drosophila; PiggyBac; GFP; Transposable elements.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012

Chromosomal distribution of the As51 satellite DNA in two species complexes of the genus Astyanax (Pisces, Characidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Abel,Luciano Douglas dos Santos; Mantovani,Monique; Moreira-Filho,Orlando.
The chromosomal localization of the As51 satellite DNA was identified by fluorescent in situ hybridization (FISH) in specimens of the characid fish Astyanax scabripinnis and Astyanax fasciatus, which are considered species complexes because of their extensive karyotypical and morphological variability. A conserved chromosomal distribution of the As51 satellite, coincident with distal C-banded segments was demonstrated. The alternative interstitial localization of this satellite DNA and possible alterations of its structure suggest that this sequence underwent quantitative, positional and structural variations, as the A. scabripinnis and A. fasciatus complexes diverged.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: As51; Astyanax scabripinnis; Astyanax fasciatus; Fluorescence in situ hybridization (FISH); C-banding; Satellite DNA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300008

Gender-specific association of the rs6499640 polymorphism in the FTO gene with plasma lipid levels in Chinese children
Provedor de dados: Genetics and Molecular Biology
Autores: Gao,Liwang; Wu,Lijun; Zhang,Meixian; Zhao,Xiaoyuan; Cheng,Hong; Mi,Jie.
Abstract The fat mass- and obesity-associated gene (FTO) is significantly associated with obesity, but the associations of FTO with obesity-related traits are not fully described. We aimed to investigate the association of the FTO single nucleotide polymorphism (SNP) rs6499640 with lipid levels in Chinese children. A total of 3503 children aged 6-18 years were included in the present study. Lipid levels were analyzed and the SNP rs6499640 was genotyped using the TaqMan Allelic Discrimination Assay. Statistically significant associations were found between rs6499640 and low-density lipoprotein cholesterol (LDL-C) (p = 0.008), total cholesterol (TC) (p = 0.005), and triglycerides (TG) (p &lt; 0.001) in girls under a dominant model adjusted for age and BMI....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Children; FTO; Lipid levels.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300397

Ecological setup, ploidy diversity, and reproductive biology of Paspalum modestum, a promising wetland forage grass from South America
Provedor de dados: Genetics and Molecular Biology
Autores: Karunarathne,Piyal; Feduzka,Cristian; Diego,Hojsgaard.
Abstract With ever-rising demand for food, forage breeding for intensification of cattle production is also taking a leap. In South America, cattle production systems are displaced to marginal areas poorly exploited with cultivated pastures yet with high potential for growing stocking rates. This places the need for using native genetic resources to breed locally adapted plant genotypes that benefits from better forage quality, yield, and lesser threat to the local biodiversity. Paspalum modestum Mez is a grass species that produces quality forage and grows in marginal areas like estuaries and floodplains, suitable for introduction in breeding programs. In this study we characterize the species' reproductive biology and ecological preferences needed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apomixis; Ecological niche; Plant breeding; Polyploidy; Sexuality.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000300304

The libraries that made SUCEST
Provedor de dados: Genetics and Molecular Biology
Autores: Vettore,André L.; Silva,Felipe R. da; Kemper,Edson L.; Arruda,Paulo.
A large-scale sequencing of sugarcane expressed sequence tags (ESTs) was carried out as a first step in depicting the genome of this important tropical crop. Twenty-six unidirectional cDNA libraries were constructed from a variety of tissues sampled from thirteen different sugarcane cultivars. A total of 291,689 cDNA clones were sequenced in their 5’ and 3’end regions. After trimming low-quality sequences and removing vector and ribosomal RNA sequences, 237,954 ESTs potentially derived from protein-encoding messenger RNA (mRNA) remained. The average insert size in all libraries was estimated to be 1,250bp with the insert length varying from 500 to 5,000 bp. Clustering the 237,954 sugarcane ESTs resulted in 43,141clusters, from which 38% had no matches with...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100002

A snapshot of gene therapy in Latin America
Provedor de dados: Genetics and Molecular Biology
Autores: Linden,Rafael; Matte,Ursula.
Gene therapy attempts the insertion and expression of exogenous genetic material in cells for therapeutic purposes. Conceived in the 1960s, gene therapy reached its first clinical trial at the end of the 1980s and by December 2013 around 600 genuine open clinical trials of gene therapy were registered at NIH Clinical Trials Database. Here, we summarize the current efforts towards the development of gene therapy in Latin America. Our survey shows that the number of scientists involved in the development of gene therapy and DNA vaccines in Latin America is still very low. Higher levels of investment in this technology are necessary to boost the advancement of innovation and intellectual property in this field in a way that would ease both the social and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene therapy; Gene transfer; Gene delivery; Viral vector; South America.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200015

Karyotypic description of the stingless bee Oxytrigona cf. flaveola (Hymenoptera, Apidae, Meliponina) of a colony from Tangará da Serra, Mato Grosso State, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Krinski,Diones; Fernandes,Anderson; Rocha,Marla Piumbini; Pompolo,Silvia das Graças.
The aim was to broaden knowledge on the cytogenetics of the subtribe Meliponina, by furnishing cytogenetic data as a contribution to the characterization of bees from the genus Oxytrigona. Individuals of the species Oxytrigona cf. flaveola, members of a colony from Tangará da Serra, Mato Grosso State, Brazil, were studied. The chromosome number was 2n = 34, distributed among four chromosomal morphologies, with the karyotype formula 8m+8sm+16st+2t. Size heteromorphism in the first metacentric pair, subsequently confirmed by sequential staining with fluorochrome (DA/DAPI/CMA3), was apparent in all the examined individuals The nucleolar organizing regions (NORs) are possibly located in this metacentric chromosome pair. These data will contribute towards a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome; Heteromorphism; Fluorochrome.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300020

Cytogenetic analysis of Astylus antis (Perty, 1830) (Coleoptera, Melyridae): Karyotype, heterochromatin and location of ribosomal genes
Provedor de dados: Genetics and Molecular Biology
Autores: Mendes-Neto,Ernani de Oliveira; Vicari,Marcelo Ricardo; Campaner,Carlos; Nogaroto,Viviane; Artoni,Roberto Ferreira; Almeida,Mara Cristina.
Cytogenetic analysis of Astylus antis using mitotic and meiotic cells was performed to characterize the haploid and diploid numbers, sex determination system, chromosome morphology, constitutive heterochromatin distribution pattern and chromosomes carrying nucleolus organizer regions (NORs). Analysis of spermatogonial metaphase cells revealed the diploid number 2n = 18, with mostly metacentric chromosomes. Metaphase I cells exhibited 2n = 8II+Xyp and a parachute configuration of the sex chromosomes. Spermatogonial metaphase cells submitted to C-banding showed the presence of small dots of constitutive heterochromatin in the centromeric regions of nearly all the autosomes and on the short arm of the X chromosome (Xp), as well as an additional band on one of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sex chromosomes; Meiosis; Metaphase; FISH; 18S rDNA.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200007

Population analysis of xenobiotic metabolizing genes in South Brazilian Euro and Afro-descendants
Provedor de dados: Genetics and Molecular Biology
Autores: Maciel,Marcos Euzébio; Oliveira,Fausto Koga; Propst,Gustavo Bonfim; Bicalho,Maria da Graça; Cavalli,Iglenir João; Ribeiro,Enilze Maria de Souza Fonseca.
Individual variability in xenobiotic metabolism has been associated with susceptibility to developing complex diseases. Genes involved in xenobiotic metabolism have been evaluated in association studies; the difficulty of obtaining accurate gene frequencies in mixed populations makes interpretation of the results difficult. We sought to estimate population parameters for the cytochrome P450 and glutathione S-transferase gene families, thus contributing to studies using these genes as markers. We describe the frequencies of six genes (CYP1A1, CYP2D6, CYP2E1, GSTM1, GSTT1, and GSTP1) and estimate population parameters in 115 Euro-descendants and 196 Afro-descendants from Curitiba, South of Brazil. PCR-based methods were used for genotyping, and statistical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CYP; GST; Population study.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400010

Population analysis of vitamin D receptor polymorphisms and the role of genetic ancestry in an admixed population
Provedor de dados: Genetics and Molecular Biology
Autores: Lins,Tulio C.; Vieira,Rodrigo G.; Grattapaglia,Dario; Pereira,Rinaldo W.
The vitamin D receptor (VDR) is an essential protein related to bone metabolism. Some VDR alleles are differentially distributed among ethnic populations and display variable patterns of linkage disequilibrium (LD). In this study, 200 unrelated Brazilians were genotyped using 21 VDR single nucleotide polymorphisms (SNPs) and 28 ancestry informative markers. The patterns of LD and haplotype distribution were compared among Brazilian and the HapMap populations of African (YRI), European (CEU) and Asian (JPT+CHB) origins. Conditional regression and haplotype-specific analysis were performed using estimates of individual genetic ancestry in Brazilians as a quantitative trait. Similar patterns of LD were observed in the 5' and 3' gene regions. However, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian population; HapMap; Haplotype; Population diversity; VDR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300003

Preferential induction of MLL (Mixed Lineage Leukemia) rearrangements in human lymphocyte cultures treated with etoposide
Provedor de dados: Genetics and Molecular Biology
Autores: Brassesco,María Sol; Montaldi,Ana Paula; Sakamoto-Hojo,Elza Tiemi.
Topoisomerase II inhibitors are effective chemotherapeutic agents in the treatment of cancer, in spite of being associated with the development of secondary leukemia. Our purpose was to determine the effects of etoposide on different genomic regions, aiming at discovering whether there are preferential sites which can be targeted by this drug in peripheral lymphocytes from healthy individuals. The in vitro treatment with low doses of etoposide (0.25, 0.5, and 1 µg/mL, in 1 hour-pulse or continuous-48 h treatment) induced a significant increase in chromosomal aberrations, detected by conventional staining and FISH with specific probes for chromosomes 8 and 11, compared with untreated controls (p &lt; 0.05). Additionally, the frequencies of alterations at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Etoposide; FISH; MLL translocations.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100022

Cytogenetic analysis of the effects of 2.5 and 10.5 GHz microwaves on human lymphocytes
Provedor de dados: Genetics and Molecular Biology
Autores: Figueiredo,André B.S.; Alves,Rex N.; Ramalho,Adriana T.
The biological effects of microwaves on living organisms remain highly controversial. Although some reports have suggested that microwaves may be directly or indirectly genotoxic, a direct action is unlikely because the low energy of microwave photons makes them unable to cause single-strand breaks in DNA. In this work, we examined the possible clastogenic properties of microwaves (2.5 and 10.5 GHz) on blood lymphocytes in vitro by monitoring the frequency of chromosomal aberrations. We also investigated whether blood cells showed increased radiosensitivity or radioresistance when pretreated with the microwaves and then irradiated with gamma radiation. There was no significant difference in the frequency of chromosomal aberrations between cells which had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal aberrations; Human lymphocytes; Microwaves.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300024

Mitochondrial genome organization and vertebrate phylogenetics
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Sérgio Luiz.
With the advent of DNA sequencing techniques the organization of the vertebrate mitochondrial genome shows variation between higher taxonomic levels. The most conserved gene order is found in placental mammals, turtles, fishes, some lizards and Xenopus. Birds, other species of lizards, crocodilians, marsupial mammals, snakes, tuatara, lamprey, and some other amphibians and one species of fish have gene orders that are less conserved. The most probable mechanism for new gene rearrangements seems to be tandem duplication and multiple deletion events, always associated with tRNA sequences. Some new rearrangements seem to be typical of monophyletic groups and the use of data from these groups may be useful for answering phylogenetic questions involving...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400008

Evaluation of genetic resources for hybrid rice (Oryza sativa L.) production
Provedor de dados: Genetics and Molecular Biology
Autores: Beló,André.
Tipo: Info:eu-repo/semantics/other
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200022

Remembering the past – studies on evolution done by the genetics group at Universidade Federal do Rio Grande do Sul (UFRGS)
Provedor de dados: Genetics and Molecular Biology
Autores: Salzano,Francisco M.
Abstract After a brief introduction about the factors that are involved in science development, and world and Brazilian evolutionary genetics, the studies developed in Porto Alegre in this area were reviewed. Four periods in the development of this group were distinguished: (a) Origins and first expansion (1949-1961); (b) Second expansion (1962-1988); (c) Third expansion (1989-2001); and (d) The last 15 years (2002-present). The international Porto Alegre Biological Evolution Workshops (PABEWs), with five biannual events from 2007 o 2015, were also mentioned. The final message stressed the importance of the maintenance of this and other Brazilian groups of research through adequate finance and recognition.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: History; Genetics research; Evolutionary genetics; Porto Alegre; Brazil.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200181

Mutagenicity of hydroxyurea in lymphocytes from patients with sickle cell disease
Provedor de dados: Genetics and Molecular Biology
Autores: Khayat,André Salim; Guimarães,Adriana Costa; Cardoso,Plínio Cerqueira; Lima,Patrícia Danielle Lima de; Bahia,Marcelo de Oliveira; Antunes,Lusânia M. Greggi; Burbano,Rommel Rodríguez.
Hydroxyurea is commonly used in the treatment of myeloproliferative diseases and in patients with sickle cell disease (SCD). The use of this antineoplastic agent in patients with SCD is justified because of the drug's ability to increase fetal hemoglobin levels, thereby decreasing the severity of SCD. However, high doses or prolonged treatment with hydroxyurea can be cytotoxic or genotoxic for these patients, with an increased risk of developing acute leukemia. This danger can be avoided by monitoring the lymphocytes of patients treated with hydroxyurea. Cytogenetic tests are important endpoints for monitoring the physiological effects of physical and chemical agents, including drugs. In this work, we assessed the genotoxicity of hydroxyurea in short-term...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydroxyurea; Sickle cell disease; Mutagenesis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100019

A genetic variant in microRNA-146a is associated with sporadic breast cancer in a Southern Brazilian Population
Provedor de dados: Genetics and Molecular Biology
Autores: Brincas,Heloisa Magagnin; Augusto,Danillo G.; Mathias,Carolina; Cavalli,Iglenir João; Lima,Rubens Silveira de; Kuroda,Flávia; Urban,Cícero de Andrade; Gradia,Daniela Fiori; de Oliveira,Jaqueline; de Almeida,Rodrigo Coutinho; Ribeiro,Enilze Maria de Souza Fonseca.
Abstract MicroRNAs (miRNAs) play an essential role in gene expression and affect the development of tumours, including breast cancer (BC). Polymorphisms in miRNA genes can affect the interaction of miRNAs with their target messenger RNA by interfering, creating or disrupting target sites. The single nucleotide polymorphism (SNP) rs2910164, located in the seed region of miR146a, was shown to be associated with BC among different populations. In the present study, we investigated whether rs2910164 is associated with BC in 326 patients and 411 controls from a Brazilian population of predominantly European ancestry. The presence of the allele rs2910164*C was associated with an increased risk of BC (OR=1.4, 95% CI=1.03-1.85, p = 0.03). We also analysed publicly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiRNA polymorphism; MiR146a; Rs2910164; Breast cancer; Case-control study.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500103

Alcohol dehydrogenase activities and ethanol tolerance in Anastrepha (Diptera, Tephritidae) fruit-fly species and their hybrids
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Eneas; Solferini,Vera Nisaka; Matioli,Sergio Russo.
The ADH (alcohol dehydrogenase) system is one of the earliest known models of molecular evolution, and is still the most studied in Drosophila. Herein, we studied this model in the genus Anastrepha (Diptera, Tephritidae). Due to the remarkable advantages it presents, it is possible to cross species with different Adh genotypes and with different phenotype traits related to ethanol tolerance. The two species studied here each have a different number of Adh gene copies, whereby crosses generate polymorphisms in gene number and in composition of the genetic background. We measured certain traits related to ethanol metabolism and tolerance. ADH specific enzyme activity presented gene by environment interactions, and the larval protein content showed an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcohol dehydrogenase; Anastrepha; Hybrids; Ethanol tolerance; Tephritidae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100027

Genetic and phenotypic variability of iris color in Buenos Aires population
Provedor de dados: Genetics and Molecular Biology
Autores: Hohl,Diana María; Bezus,Brenda; Ratowiecki,Julia; Catanesi,Cecilia Inés.
Abstract The aim of this work was to describe the phenotypic and genotypic variability related to iris color for the population of Buenos Aires province (Argentina), and to assess the usefulness of current methods of analysis for this country. We studied five Single Nucleotide Polymorphisms (SNPs) included in the IrisPlex kit, in 118 individuals, and we quantified eye color with Digital Iris Analysis Tool. The markers fit Hardy-Weinberg equilibrium for the whole sample, but not for rs12913832 within the group of brown eyes (LR=8.429; p=0.004). We found a remarkable association of HERC2 rs12913832 GG with blue color (p &lt; 0.01) but the other markers did not show any association with iris color. The results for the Buenos Aires population differ from those...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population genetics; Iris color; Forensic genetics; Phenotype determination; Argentinian population.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100050

A search for markers of sugarcane evolution
Provedor de dados: Genetics and Molecular Biology
Autores: Bacci Jr.,M.; Miranda,V.F.O.; Martins,V.G.; Figueira,A.V.O.; Lemos,M.V.; Pereira,J.O.; Marino,C.L.
To determine the phylogenetic relationship between sugarcane cultivars and other members of the Saccharinae subtribe, we identified the fast evolving ITS1-5.8S-ITS2 (ITS = internal transcribed spacer; 5.8S = 5.8S ribosomal DNA) region of the sugarcane genome in the Sugarcane Expressed Sequence Tag (SUCEST) genome project database. Parsimony analysis utilizing this region and homologs belonging to the 23 closely related Andropogoneae currently deposited in the GenBank database has shown sugarcane as the sister group of Saccharum sinense. However, because there are few parsimony-informative characters and high homoplasy in the ITS1-5.8S-ITS2 region we were not able to determine with confidence the phylogenetic relationship between sugarcane and some of the...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100023

Erratum
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400725

Shaping a Plant Genetics and Molecular Biology research community in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Sluys,Marie-Anne Van.
Tipo: Info:eu-repo/semantics/other
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200001

Butyrylcholinesterase genetic variability in Guarani Amerindians from the Brazilian state of Mato Grosso do Sul
Provedor de dados: Genetics and Molecular Biology
Autores: Furtado,Lupe; Souza,Ricardo L. R.; Tsuneto,Luiza T.; Petzl-Erler,Maria Luiza; Chautard-Freire-Maia,Eleidi A.
Human butyrylcholinesterase (BChE; EC 3.1.1.8) is a polymorphic enzyme coded by the BCHE gene (3q26.1-q26.2) while the CHE2 gene (2q33-q35) determines a still not characterized substance that forms a complex with BChE (C5), being the CHE2 C5+ and CHE2 C5- phenotypes detected in electrophoresis. The present study investigated BCHE and CHE2 variability and the BChE activity of Brazilian Guarani Amerindians from the Kaiowá and Ñandeva sub-groups living in several indigenous territories in the Brazilian state of Mato Grosso do Sul. The frequency of the BCHE exon 2 D70G (A) allele was 0.60% ± 0.35% while that of the BCHE exon 2 G390V (F-2) allele, never before screened in Amerindians, was 8.82% ± 1.35%. This is the first time that the BCHE gene exon 4 A539T (K)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BCHE gene; A allele; F-2 and K alleles; Guarani-Kaiowá; Guarani-Ñandeva.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100002

Mucopolysaccharidoses in northern Brazil: Targeted mutation screening and urinary glycosaminoglycan excretion in patients undergoing enzyme replacement therapy
Provedor de dados: Genetics and Molecular Biology
Autores: Viana,Gustavo M.; Lima,Nathália O. de; Cavaleiro,Rosely; Alves,Erik; Souza,Isabel C.N.; Feio,Raimunda; Leistner-Segal,Sandra; Schwartz,Ida; Giugliani,Roberto; Silva,Luiz C. Santana da.
Mucopolysaccharidoses (MPS) are rare lysosomal disorders caused by the deficiency of specific lysosomal enzymes responsible for glycosaminoglycan (GAG) degradation. Enzyme Replacement Therapy (ERT) has been shown to reduce accumulation and urinary excretion of GAG, and to improve some of the patients' clinical signs. We studied biochemical and molecular characteristics of nine MPS patients (two MPS I, four MPS II and three MPS VI) undergoing ERT in northern Brazil. The responsiveness of ERT was evaluated through urinary GAG excretion measurements. Patients were screened for eight common MPS mutations, using PCR, restriction enzyme tests and direct sequencing. Two MPS I patients had the previously reported mutation p.P533R. In the MPS II patients, mutation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucopolysaccharidosis; Enzyme replacement therapy; Mutations; Glycosaminoglycans.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300007

Identifying Mazama gouazoubira (Artiodactyla; Cervidae) chromosomes involved in rearrangements induced by doxorubicin
Provedor de dados: Genetics and Molecular Biology
Autores: Tomazella,Iara Maluf; Abril,Vanessa Veltrini; Duarte,José Maurício Barbanti.
Abstract The process of karyotype evolution in Cervidae from a common ancestor (2n = 70, FN = 70) has been marked by complex chromosomal rearrangements. This ancestral karyotype has been retained by the current species Mazama gouazoubira (Fischer 1814), for which a chromosomal polymorphism (Robertsonian translocations and the presence of B chromosomes) has been described, presumably caused by a chromosome fragility. Thus, this study has identified doxorubicin-induced chromosome aberrations and mapped the regions involved in breaks, which may be related to the chromosome evolution process. G-banding pattern showed that 21 pairs of chromosomes presented chromosomal aberrations, 60% of the total chromosome number of the species M. gouazoubira. Among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal aberrations; Chromosome evolution; Brown brocket deer; G-banding.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300460

In silico identification of coffee genome expressed sequences potentially associated with resistance to diseases
Provedor de dados: Genetics and Molecular Biology
Autores: Alvarenga,Samuel Mazzinghy; Caixeta,Eveline Teixeira; Hufnagel,Bárbara; Thiebaut,Flávia; Maciel-Zambolim,Eunize; Zambolimand,Laércio; Sakiyama,Ney Sussumu.
Sequences potentially associated with coffee resistance to diseases were identified by in silico analyses using the database of the Brazilian Coffee Genome Project (BCGP). Keywords corresponding to plant resistance mechanisms to pathogens identified in the literature were used as baits for data mining. Expressed sequence tags (ESTs) related to each of these keywords were identified with tools available in the BCGP bioinformatics platform. A total of 11,300 ESTs were mined. These ESTs were clustered and formed 979 EST-contigs with similarities to chitinases, kinases, cytochrome P450 and nucleotide binding site-leucine rich repeat (NBS-LRR) proteins, as well as with proteins related to disease resistance, pathogenesis, hypersensitivity response (HR) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea; Data mining; ESTs; Genomics; In silico; Bioinformatics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400031

A cytological study of the O5 chromosomal inversion of Drosophila subobscura (Diptera, Drosophilidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Mestres,Francesc; Figueras,Maria Teresa; Serra,Luís.
The O5 chromosomal inversion has been a cornerstone for understanding different aspects of the American colonization by Drosophila subobscura. To obtain more information of this evolutionary event it is important to know the pattern of bands of this inversion in detail. Comparing this pattern with that of D. melanogaster it is possible to predict which genes are located inside or close to the O5 inversion and use them as genetic markers. In this study, the complete band pattern of the O5 inversion is presented. Furthermore, the most important genes located inside it have been predicted. Finally, a constriction located close to the proximal breakage point of the O5 inversion has been observed many times and its possible genetic significance is discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosohila subobscura; Chromosomal inversion; Colonization; Lethal genes.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400007

Chromosome comparison between two species of Phyllostomus (Chiroptera - Phyllostomidae) from Eastern Amazonia, with some phylogenetic insights
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Luís R.R.; Barros,Regina M.S.; Assis,Maria de Fátima L.; Marques-Aguiar,Suely A.; Pieczarka,Julio C.; Nagamachi,Cleusa Y.
The karyotypes of Phyllostomus discolor and P. hastatus from Eastern Amazonia were studied by G-, C-, G/C sequential and Ag-NOR techniques. Both species presented 2n = 32, with the autosome complement composed of 30 bi-armed in P. discolor and 28 bi-armed plus 1 acrocentric in P. hastatus. In both species, the X chromosome is medium submetacentric while the Y is minute acrocentric. The present study found only one difference between the karyotypes of P. discolor and P. hastatus: the smallest autosome (pair 15) is bi-armed in discolor and acrocentric in hastatus, a result best explained by pericentric inversion. The C-banding revealed constitutive heterochromatin only at the centromeric regions of all chromosomes, with the NOR site located at the distal...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300016

Molecular typing of HLA class II antigens in a São Paulo population
Provedor de dados: Genetics and Molecular Biology
Autores: Goldberg,A.C.; Chiarella,J.M.; Marin,M.L.C.; Rosales,C.; Banic,D.; Oliveira,M.A.; Rodrigues,H.; Viggiani,C.S.; Kalil,J.
In the present paper we show data obtained from a normal population with a racially mixed profile typical of the city of São Paulo, State of São Paulo. Data were generated with polymerase chain reaction using sequence specific primers (PCR-SSP) for HLA-DRB and polymerase chain reaction followed by hybridization with sequence specific oligonucleotide probes (PCR-SSO) for HLA-DQA1 and HLA-DQB1 loci. HLA-DRB, DQA1, DQB1 and haplotype frequencies as well as common linkage disequilibria were found. This population was also shown to be in genetic equilibrium according to the Hardy-Weinberg law. HLA-DR typing of a normal sample from the city of Porto Velho, State of Rondonia, highlighted the importance of different sets of HLA profiles found in other regions of...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300001

Genetic and morphometric differences between yellowtail snapper (Ocyurus chrysurus, Lutjanidae) populations of the tropical West Atlantic
Provedor de dados: Genetics and Molecular Biology
Autores: Vasconcellos,Anderson V.; Vianna,Paulo; Paiva,Paulo C.; Schama,Renata; Solé-Cava,Antonio.
Populations of Ocyurus chrysurus were compared genetically and morphometrically along the West Atlantic coast to test the null hypothesis of population homogeneity in the area. Brazilian populations were found to be differentiated in shape (canonical variates analysis; F[48,515] = 10.84, p &lt; 0.0001). Analyses of mitochondrial DNA sequences (663 bp of the control region) did not show any differences between Brazilian populations but could detect differences between Brazilian and Caribbean (Belize) populations. The samples from Pernambuco differed significantly from the other Brazilian populations in allozyme frequencies (11 loci; F ST = 0.167; p &lt; 0.05), but this may have resulted from the small number of samples analysed for that population. Sequence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; Allozymes; Morphometry; Fisheries; Phylogeography.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200026

Proteomic analysis of halotolerant proteins under high and low salt stress in Dunaliella salina using two-dimensional differential in-gel electrophoresis
Provedor de dados: Genetics and Molecular Biology
Autores: Jia,Yan-Long; Chen,Hui; Zhang,Chong; Gao,Li-Jie; Wang,Xi-Cheng; Qiu,Le-Le; Wu,Jun-Fang.
Abstract Dunaliella salina, a single-celled marine alga with extreme salt tolerance, is an important model organism for studying fundamental extremophile survival mechanisms and their potential practical applications. In this study, two-dimensional differential in-gel electrophoresis (2D-DIGE) was used to investigate the expression of halotolerant proteins under high (3 M NaCl) and low (0.75 M NaCl) salt concentrations. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) and bioinformatics were used to identify and characterize the differences among proteins. 2D-DIGE analysis revealed 141 protein spots that were significantly differentially expressed between the two salinities. Twenty-four differentially...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dunaliella salina; Halotolerant protein; Proteomics; Two-dimensional differential in-gel electrophoresis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200239

Genetic structure of the sea-bob shrimp (Xiphopenaeus kroyeri Heller, 1862; Decapoda, Penaeidae) along the Brazilian southeastern coast
Provedor de dados: Genetics and Molecular Biology
Autores: Voloch,Carolina Moreira; Solé-Cava,Antonio Mateo.
The sea-bob shrimp, Xiphopenaeus kroyeri, is one of the most important economic marine resources along the entire Brazilian coast. Nevertheless, despite its economic importance, no studies have examined the population genetics of this species. In this paper, we used ten allozyme loci to study the pattern of genetic structuring in X. kroyeri along the southeastern Brazilian coast. Seven of the ten analyzed loci were polymorphic, yielding observed heterozygosity values higher than those reported for other penaeid shrimps. The population from São Paulo was significantly different from the other two populations (Rio de Janeiro and Espírito Santo), which, in turn, seem to form a single panmitic unit. Therefore, our results clearly indicate that conservation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population genetics; Allozyme electrophoresis; Fisheries stocks; Crustacea.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200013

Nitrogen assimilation in Citrus based on CitEST data mining
Provedor de dados: Genetics and Molecular Biology
Autores: Wickert,Ester; Marcondes,Jackson; Lemos,Manoel Victor; Lemos,Eliana G.M.
Assimilation of nitrate and ammonium are vital procedures for plant development and growth. From these primary paths of inorganic nitrogen assimilation, this metabolism integrates diverse paths for biosynthesis of macromolecules, such as amino acids and nucleotides, and the central intermediate metabolism, like carbon metabolism and photorespiration. This paper reports research performed in the CitEST (Citrus Expressed Sequence Tag) database for the main genes involved in nitrogen metabolism and those previously described in other organisms. The results show that a complete cluster of genes involved in the assimilation of nitrogen and the metabolisms of glutamine, glutamate, aspartate and asparagine can be found in the CitEST data. The main enzymes found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitrogen assimilation; Citrus; CitEST datamining.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500009

Intrinsic structural variation of the complex microsatellite marker MYCL1 in Finnish and Somali populations and its relevance to gastrointestinal tumors
Provedor de dados: Genetics and Molecular Biology
Autores: Vauhkonen,Hanna; Sajantila,Antti.
The structurally complex MYCL1 microsatellite marker is often used to determine microsatellite instability in colorectal cancers but the allelic variation of this marker has remained largely uncharacterized in both populations and in cancers. Our study describes the allelic distributions of MYCL1 in Finnish (n = 117) and Somali population samples (n = 61) of non-related individuals and compares this distribution with the instability pattern obtained from 61 gastrointestinal tumors.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colorectal cancers; Complex microsatellite; Gastrointestinal tumors; Microsatellite instability; Finnish and Somali populations.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400005

Changes in G+C content of a neutrally evolving gene under a non-reversible dynamics measured by computer simulations based on experimental evolution data
Provedor de dados: Genetics and Molecular Biology
Autores: Brunstein,Adriana; Varuzza,Leonardo; Sanson,Gerdine F. O.; Briones,Marcelo R. S.
To evaluate the effects of non-reversibility on compositional base changes and the distribution of branch lengths along a phylogeny, we extended, by means of computer simulations, our previous sequential PCR in vitro evolution experiment. In that study a 18S rRNA gene evolved neutrally for 280 generations and a homogeneous non-stationary model of base substitution based on a non-reversible dynamics was built from the in vitro evolution data to describe the observed pattern of nucleotide substitutions. Here, the process was extended to 840 generations without selection, using the model parameters calculated from the in vitro evolution experiment. We observed that under a non-reversible model the G+C content of the sequences significantly increases when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular evolution; Experimental phylogenetics; Neutral theory; Mathematical models of nucleotide substitutions.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400026

Karyotypic characterization of Ramphastidae (Piciformes, Aves)
Provedor de dados: Genetics and Molecular Biology
Autores: Castro,Márcio Siqueira; Recco-Pimentel,Shirlei M.; Rocha,Guaracy Tadeu.
The karyotypes of nine species of the family Ramphastidae were determined and compared with that of the toco toucan (Ramphastos toco), the only ramphastid karyotype so far reported in the literature. Differences in the morphology of the largest chromosomes allowed to identify three karyotype groups. The first group included the species R. toco, Baillonius bailloni, Pteroglossus castanotis, P. aracari and Selenidera maculirostris, in which only the first pair of chromosomes was metacentric. The second group included four Ramphastos species (R. dicolorus, R. ariel, R. vitellinus, R. tucanus cuvieri) with two pairs of metacentric macrochromosomes (the first and the seventh). The third group was represented by a single species, A. laminirostris, in which all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotypes; Rhamphastidae; Toucans.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200006

Molecular surface directionality of the DNA-binding protein surface on the earth map
Provedor de dados: Genetics and Molecular Biology
Autores: Lee,Wei-Po; Tzou,Wen-Shyong.
Protein-DNA interactions play a pivotal role in both the transcriptional control and the maintenance of genome integrity, and these are two properties that are closely linked to the development of an organism, differentiation, physiology and to the progression of diseases. Chemical and geometric properties are typically two of the key components in any analysis that aims to understand the precise origin of specificity and elucidate the atomic features of a protein-DNA interface. In this study, we have developed a unique representation of the directionality of the molecular surface of a DNA-binding protein. The stereo-orientation of the normal vector that signifies the geometric properties of a protein surface was projected onto a two-dimensional surface...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Normal vector; Molecular surface; Protein-DNA interaction; Earth map.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200033

Detection of trisomy 12 by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia
Provedor de dados: Genetics and Molecular Biology
Autores: Chauffaille,Maria de Lourdes L.F.; Marques,Eliana Azevedo; Oliveira,Jose Salvador Rodrigues de; Rodrigues,Maria Madalena; Figueiredo,Maria Stella; Romeo,Maura; Yamamoto,Mihoko; Kerbauy,José.
Chronic lymphocytic leukemia (CLL) presents a varying incidence of karyotypic abnormalities whose detection is complicated by difficulties in obtaining mitosis for analysis in this type of mature lymphocyte disorder. Since the introduction of molecular cytogenetics (FISH = fluorescent in situ hybridization), applying centromeric probes for chromosome 12 has made it possible to detect a higher percentage of trisomy 12 cases. The objective of the present study was to detect trisomy 12 by FISH (alpha satellite probe) in 13 patients with CLL whose karyotypes by G-banding were either normal or inadequate. Using this method trisomy 12 was detected in three patients in a percentage of positive cells varying from 55.5% to 79%, showing that FISH is a sensitive and...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300005

Effect of polymorphisms in the Slc11a1 coding region on resistance to brucellosis by macrophages in vitro and after challenge in two Bos breeds (Blanco Orejinegro and Zebu)
Provedor de dados: Genetics and Molecular Biology
Autores: Martínez,Rodrigo; Dunner,Susana; Toro,Rubén; Tobón,Jaime; Gallego,Jaime; Cañón,Javier.
The resistance/susceptibility of selected cattle breeds to brucellosis was evaluated in an F1 population generated by crossing animals classified as resistant (R) and susceptible (S) (R x R, R x S, S x R, S x S) based on challenges in vitro and in vivo. The association between single nucleotide polymorphisms identified in the coding region of the Slc11a1 gene and resistance/susceptibility was estimated. The trait resistance or susceptibility to brucellosis, evaluated by a challenge in vitro, showed a high heritable component in terms of additive genetic variance (h² = 0.54 ± 0.11). In addition, there was a significant association (p &lt; 0.05) between the control of bacterial survival and two polymorphisms (a 3'UTR and SNP4 located in exon 10). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blanco Orejinegro Creole breed; Brahman breed; Brucella abortus; Genetic resistance; Zebu.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300014

Genetic characterization of Aberdeen Angus cattle using molecular markers
Provedor de dados: Genetics and Molecular Biology
Autores: Vasconcellos,Luciana Pimentel de Mello Klocker; Tambasco-Talhari,Daniella; Pereira,Andréa Pozzi; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida.
Aberdeen Angus beef cattle from the Brazilian herd were studied genetically using restriction fragment length polymorphism (RFLP) of the kappa-casein - HinfI (CSN3 - HinfI), beta-lactoglobulin - HaeIII (LGB - HaeIII) and growth hormone AluI (GH- AluI) genes, as well as four microsatellites (TEXAN15, CSFM50, BM1224 and BM7160). The RFLP genotypes were determined using the polymerase chain reaction (PCR) followed by digestion with restriction endonucleases and electrophoresis in agarose gels. With the exception of the microsatellite BM7160, which was analyzed in an automatic sequencer, the PCR products were genotyped by silver staining. The allele and genotype frequencies, heterozygosities and gene diversity were estimated. The values for these parameters of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aberdeen Angus; Bovine; DNA; Characterization; RFLP; Microsatellite.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200005

Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti &amp;amp; Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Fernandes,Carlos Alexandre; Martins-Santos,Isabel Cristina.
Fluorescence in situ hybridization (FISH) was undertaken in order to determinate the chromosomal distribution pattern of 18S and 5S ribosomal DNAs (rDNA) in four populations of the characid fish Astyanax altiparanae from the upper Paraná river basin, Brazil. The 18S rDNA probe FISH revealed numerical and positional variations among specimens from the Keçaba stream compared to specimens of the other populations studied. In contrast to the variable 18S rDNA distribution pattern, highly stable chromosomal positioning of the 5S rDNA sites was observed in the four A. altiparanae populations. Divergence in the distribution pattern of 18S and 5S rDNA sites is also discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astyanax altiparanae; Fluorescence in situ hybridization (FISH); 18S rDNA; 5S rDNA; Sequential Ag-NOR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300011

Sequence diversity and copy number variation of Mutator-like transposases in wheat
Provedor de dados: Genetics and Molecular Biology
Autores: Asakura,Nobuaki; Yoshida,Shinya; Mori,Naoki; Ohtsuka,Ichiro; Nakamura,Chiharu.
Partial transposase-coding sequences of Mutator-like elements (MULEs) were isolated from a wild einkorn wheat, Triticum urartu, by degenerate PCR. The isolated sequences were classified into a MuDR or Class I clade and divided into two distinct subclasses (subclass I and subclass II). The average pair-wise identity between members of both subclasses was 58.8% at the nucleotide sequence level. Sequence diversity of subclass I was larger than that of subclass II. DNA gel blot analysis showed that subclass I was present as low copy number elements in the genomes of all Triticum and Aegilops accessions surveyed, while subclass II was present as high copy number elements. These two subclasses seemed uncapable of recognizing each other for transposition. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutator-like transposase; Sequence diversity; Copy number variation; Triticum; Aegilops.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300022

Mutagenic activation of CL64,855, an anti-Trypanosoma cruzi nitroderivant, by bacterial nitroreductases
Provedor de dados: Genetics and Molecular Biology
Autores: Morais Jr.,Marcos Antonio de; Ferreira,Rita de Cássia Café; Ferreira,Luiz Carlos de Souza.
CL64,855 is a nitroimidazole-thiodiazole derivate with high anti-Trypanosoma cruzi activity. CL64,855-induced mutagenesis in the Salmonella/microsome test was detected by TA98 and TA98dnp6 strains, but not by the nitroreductase I-deficient TA98nr strain. The lack of mutagenic response of TA98nr was connected with its extreme resistance to the killing effect of the drug. Presence of S9 mix did not restore mutagenic activity of CL64,855 to the TA98nr strain. Additionally, CL64,855 was reduced in vitro by the nitroreductase I-proficient TA98 strain, mainly in the presence of oxygen, but not by the TA98nr strain. Mutagenic activity was detected in serum samples of treated guinea pigs by nitroreductase-proficient strains TA98 and TA98dnp6, but not by...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400026

The 3' terminal sequence of the inosine monophosphate dehydrogenase gene encodes an active domain in the yeast Schizosaccharomyces pombe
Provedor de dados: Genetics and Molecular Biology
Autores: Karaer,Semian; Sarikaya,Aysegül Topal; Arda,Nazli; Temizkan,Güler.
The gua1 gene encoding inosine monophosphate dehydrogenase (IMPDH), which catalyses the first step in de novo biosynthesis of guanosine monophosphate (GMP), was cloned in the yeast Schizosaccharomyces pombe by functional complementation of a gua1ura4-D18 mutant strain from a S. pombe DNA genomic library. Complementation analysis revealed a 1.2 kb fragment which segregation analysis confirmed did not code for a suppressor gene. Only 446 nucleotides of the gua1 gene encoding the IMPDH C-terminal residues were found within this 1.2 kb sequence (GenBank, AJ293460). The comparison of this wild-type fragment with the same fragment from the gua1ura4-D18 mutant revealed that there was a point mutation at position 1261 (guanine -&gt; adenine) from the 5' end,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schizosaccharomyces pombe; Inosine monophosphate dehydrogenase; Gua1 gene; Purine nucleotide pathway.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300026

Zero-inflated Poisson regression models for QTL mapping applied to tick-resistance in a Gyr x Holstein F2 population
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Fabyano Fonseca; Tunin,Karen P.; Rosa,Guilherme J.M.; Silva,Marcos V.B. da; Azevedo,Ana Luisa Souza; Verneque,Rui da Silva; Machado,Marco Antonio; Packer,Irineu Umberto.
Nowadays, an important and interesting alternative in the control of tick-infestation in cattle is to select resistant animals, and identify the respective quantitative trait loci (QTLs) and DNA markers, for posterior use in breeding programs. The number of ticks/animal is characterized as a discrete-counting trait, which could potentially follow Poisson distribution. However, in the case of an excess of zeros, due to the occurrence of several noninfected animals, zero-inflated Poisson and generalized zero-inflated distribution (GZIP) may provide a better description of the data. Thus, the objective here was to compare through simulation, Poisson and ZIP models (simple and generalized) with classical approaches, for QTL mapping with counting phenotypes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy; Cattle; Tick infestation; QTL regression; Generalized linear model.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400008

Genetic diversity of b-glucuronidase activity among 14 strains of the dominant human gut anaerobe Ruminococcus gnavus
Provedor de dados: Genetics and Molecular Biology
Autores: Beaud,Diane; Ladiré,Monique; Azevedo,Vasco; Bridonneau,Chantal; Anba-Mondoloni,Jamila.
Bacterial beta-glucuronidase activity in the gut increases the enterohepatic circulation of toxic compounds and plays a major role in the etiology of colon cancer. Previously, we had found that the gus gene, which codes for beta-glucuronidase in a dominant anaerobic species of the gut microbiota, Ruminococcus gnavus strain E1, is transcribed as part of an operon that includes three ORFs that code for beta-glucoside permeases of the phosphotransferase systems. This genetic organization had never been described. We have now compared beta-glucuronidase activity and the genetic environment of the gus gene in 14 strains of Ruminococcus gnavus.We found that five out of the seven glucuronidase-positive R. gnavus strains possessed another glucuronidase gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ruminococcus gnavus; Glucuronidases; Digestive microbiota.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200026

Studies of micronuclei and other nuclear abnormalities in red blood cells of Colossoma macropomum exposed to methylmercury
Provedor de dados: Genetics and Molecular Biology
Autores: Rocha,Carlos Alberto Machado da; Cunha,Lorena Araújo da; Pinheiro,Raul Henrique da Silva; Bahia,Marcelo de Oliveira; Burbano,Rommel Mario Rodríguez.
The frequencies of micronuclei (MN) and morphological nuclear abnormalities (NA) in erythrocytes in the peripheral blood of tambaqui (Colossoma macropomum), treated with 2 mg.L-1 methylmercury (MeHg), were analyzed. Two groups (nine specimens in each) were exposed to MeHg for different periods (group A - 24 h; group B - 120 h). A third group served as negative control (group C, untreated; n = 9). Although, when compared to the control group there were no significant differences in MN frequency in the treated groups, for NA, the differences between the frequencies of group B (treated for 120 h) and the control group were extremely significant (p &lt; 0.02), thus demonstrating the potentially adverse effects of MeHg on C. macropomum erythrocytes after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Genotoxicity; Methylmercury; Micronuclei assay.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400024

Interleukin-8-251T &gt; a, interleukin-1α-889C &gt; t and apolipoprotein e polymorphisms in Alzheimer's disease
Provedor de dados: Genetics and Molecular Biology
Autores: Vendramini,Alex Augusto; Lábio,Roger Willian de; Rasmussen,Lucas Trevizani; Reis,Nathali Mattiuzo dos; Minett,Thais; Bertolucci,Paulo Henrique Ferreira; Pinhel,Marcela Augusta de Souza; Souza,Dorotéia Rossi Silva; Mazzotti,Diego Robles; Smith,Marília de Arruda Cardoso; Payão,Spencer Luiz Marques.
An inflammatory process has been involved in numerous neurodegenerative disorders such as Parkinson's disease, stroke and Alzheimer's disease (AD). In AD, the inflammatory response is mainly located in the vicinity of amyloid plaques. Cytokines, such as interleukin-8 (IL-8) and interleukin-1α (IL-1α), have been clearly involved in this inflammatory process. Polymorphisms of several interleukin genes have been correlated to the risk of developing AD. The present study investigated the association of AD with polymorphisms IL-8 -251T &amp;gt; A (rs4073) and IL-1α-889C &amp;gt; T (rs1800587) and the interactive effect of both, adjusted by the Apolipoprotein E genotype. 199 blood samples from patients with AD, 146 healthy elderly controls and 95 healthy young...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IL-8; IL-1α; Alzheimer's Disease; APOE; Inflammatory response.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100001

Genetic variability of 10 microsatellite markers in the characterization of Brazilian Nellore cattle (Bos indicus)
Provedor de dados: Genetics and Molecular Biology
Autores: Cervini,Marcelo; Henrique-Silva,Flávio; Mortari,Norma; Matheucci Jr,Euclides.
We assessed the polymorphism of 10 microsatellites in Brazilian Nellore cattle (Bos indicus) using a commercial multiplex system. Allele frequencies, polymorphism information content, heterozygosity and exclusion probability were calculated. Allele frequencies revealed that in the sample analyzed the markers were not equally polymorphic. The exclusion probabilities and the polymorphism information content of some loci in Nellore cattle were lower than in Bos taurus breeds. When all the microsatellites were considered the combined exclusion probability was 0.9989. This multiplex analysis can contribute toward pedigree information, adequate genetic improvements and breeding programs.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alleles; Frequencies; Microsatellite; Nellore; Polymorphism; Zebu.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300015

Increased risk of venous thrombosis by AB alleles of the ABO blood group and Factor V Leiden in a Brazilian population
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Magaly B.P.L.V.; Oliveira-Filho,Aldemir Branco de; Campos,Júlia F.; Melo,Fárida C.B.C.; Neves,Washington Batista das; Melo,Raul Antônio Morais; Lemos,José Alexandre Rodrigues.
Most cases of a predisposition to venous thrombosis are caused by resistance to activated protein C, associated in 95% of cases with the Factor V Leiden allele (FVL or R506Q). Several recent studies report a further increased risk of thrombosis by an association between the AB alleles of the ABO blood group and Factor V Leiden. The present study investigated this association with deep vein thrombosis (DVT) in individuals treated at the Hemocentro de Pernambuco in northeastern Brazil. A case-control comparison showed a significant risk of thrombosis in the presence of Factor V Leiden (OR = 10.1), which was approximately doubled when the AB alleles of the ABO blood group were present as well (OR = 22.3). These results confirm that the increased risk of deep...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ABO blood group; Factor V Leiden; Venous thrombosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200010

New soybean (Glycine max Fabales, Fabaceae) sources of qualitative genetic resistance to Asian soybean rust caused by Phakopsora pachyrhizi (Uredinales, Phakopsoraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Pierozzi,Pedro Henrique Braga; Ribeiro,Aliny Simony; Moreira,José Ubirajara Vieira; Laperuta,Larissa Di Cássia; Rachid,Breno Francovig; Lima,Wilmar Ferreira; Arias,Carlos Alberto Arrabal; Oliveira,Marcelo Fernandes de; Toledo,José Francisco Ferraz de.
Asian soybean rust (ASR), caused by the phytopathogenic fungi Phakopsora pachyrhizi, has caused large reductions in soybean (Glycine max) yield in most locations in Brazil where it has occurred since it was first reported in May 2001. Primary efforts to combat the disease involve the development of resistant cultivars, and four dominant major genes (Rpp1, Rpp2, Rpp3 and Rpp4) controlling resistance to ASR have been reported in the literature. To develop new long-lasting soybean ASR resistance genes, we used field experiments to assess ASR leaf lesion type in 11 soybean genotypes (BR01-18437, BRS 184, BRS 231, BRS 232, BRSGO Chapadões, DM 339, Embrapa 48, PI 200487, PI 230970, PI 459025-A and PI 200526) and the 55 F2 generations derived from their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disease resistance; Genetic inheritance; Major genes; Phakopsora pachyrhizi.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300018

Anti-topoisomerase drugs as potent inducers of chromosomal aberrations
Provedor de dados: Genetics and Molecular Biology
Autores: Bassi,Loredana; Palitti,Fabrizio.
DNA topoisomerases catalyze topological changes in DNA that are essential for normal cell cycle progression and therefore they are a preferential target for the development of anticancer drugs. Anti-topoisomerase drugs can be divided into two main classes: "cleavable complex" poisons and catalytic inhibitors. The "cleavable complex" poisons are very effective as anticancer drugs but are also potent inducers of chromosome aberrations so they can cause secondary malignancies. Catalytic inhibitors are cytotoxic but they do not induce chromosome aberrations. Knowledge about the mechanism of action of topoisomerase inhibitors is important to determine the best anti-topoisomerase combinations, with a reduced risk of induction of secondary malignancies.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400052

CitEST libraries
Provedor de dados: Genetics and Molecular Biology
Autores: Targon,Maria Luísa P. Natividade; Takita,Marco Aurélio; Amaral,Alexandre M. do; Souza,Alessandra A. de; Locali-Fabris,Eliane Cristina; Dorta,Sílvia de Oliveira; Borges,Kleber Martins; Souza,Juliana Mendonça de; Rodrigues,Carolina Munari; Lucheta,Adriano Reis; Freitas-Astúa,Juliana; Machado,Marcos Antonio.
In order to obtain a better understanding of what is citrus, 33 cDNA libraries were constructed from different citrus species and genera. Total RNA was extracted from fruits, leaves, flowers, bark, seeds and roots, and subjected or not to different biotic and abiotic stresses (pathogens and drought) and at several developmental stages. To identify putative promoter sequences, as well as molecular markers that could be useful for breeding programs, one shotgun library was prepared from sweet orange (Citrus sinensis var. Olimpia). In addition, EST libraries were also constructed for a citrus pathogen, the oomycete Phythophthora parasitica in either virulent or avirulent form. A total of 286,559 cDNA clones from citrus were sequenced from their 5’ end,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ESTs; Shotgun; Citrus; Phytopathogen.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500030

The beliefs of mothers in southern Brazil regarding risk-factors associated with congenital abnormalities
Provedor de dados: Genetics and Molecular Biology
Autores: Garcias,Gilberto de Lima; Schüler-Faccini,Lavínia.
We describes a study in which conventional wisdom about congenital malformations was investigated, the two main objectives being to list mothers' ideas by comparing six well-known causes of congenital abnormalities (ingestion of alcoholic beverages, smoking tobacco, drug use, marriage between relatives (consanguinity), rubella infection, maternal age) with explanations current in the general population and to discover the most common fallacies held by the general population concerning such abnormalities. The data were collected from 1 January to 31 December 1995 during a population study in which mothers were interviewed in hospitals in the city of Pelotas (population 400,000), Rio Grande do Sul state, Brazil. On each day of the study, the first three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Congenital abnormalities; Beliefs; Superstition; Pregnancy; Teratogen.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200003

Genomic growth curves of an outbred pig population
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Fabyano Fonseca e; Resende,Marcos Deon V. de; Rocha,Gilson Silvério; Duarte,Darlene Ana S.; Lopes,Paulo Sávio; Brustolini,Otávio J.B.; Thus,Sander; Viana,José Marcelo S.; Guimarães,Simone E.F.
In the current post-genomic era, the genetic basis of pig growth can be understood by assessing SNP marker effects and genomic breeding values (GEBV) based on estimates of these growth curve parameters as phenotypes. Although various statistical methods, such as random regression (RR-BLUP) and Bayesian LASSO (BL), have been applied to genomic selection (GS), none of these has yet been used in a growth curve approach. In this work, we compared the accuracies of RR-BLUP and BL using empirical weight-age data from an outbred F2 (Brazilian Piau X commercial) population. The phenotypes were determined by parameter estimates using a nonlinear logistic regression model and the halothane gene was considered as a marker for evaluating the assumptions of the GS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bayesian LASSO; Nonlinear regression; SNP effects.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400010

Non-homologous DNA end joining in normal and cancer cells and its dependence on break structures
Provedor de dados: Genetics and Molecular Biology
Autores: Poplawski,Tomasz; Pastwa,Elzbieta; Blasiak,Janusz.
DNA double-strand breaks (DSBs) are a serious threat to the cell, for if not or miss-repaired, they can lead to chromosomal aberration, mutation and cancer. DSBs in human cells are repaired via non-homologous DNA end joining (NHEJ) and homologous recombination repair pathways. In the former process, the structure of DNA termini plays an important role, as does the genetic constitution of the cells, through being different in normal and pathological cells. In order to investigate the dependence of NHEJ on DSB structure in normal and cancer cells, we used linearized plasmids with various, complementary or non-complementary, single-stranded or blunt DNA termini, as well as whole-cell extract isolated from normal human lymphocytes, chronic myeloid leukemia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA repair; Non-homologous DNA end joining; DNA double-strand breaks; Complementary and non-complementary DNA ends.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200027

Urokinase gene 3'-UTR T/C polymorphism is not associated with bladder cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Hsi-Chin,Wu; Chao-Hsiang,Chang; Wen-Chi,Chen; Huey-Yi,Chen; Fuu-Jen,Tsai.
Urokinase degrades basement proteins and is hypothesized to play a role in cancer progression. We investigated the hypothesis of C/T polymorphism in the 3'-untranslated region (3'-UTR) of the urokinase gene being associated with the development of bladder cancer. Such an association seems unlikely, since the genotype distributions in 114 bladder cancer patients did not differ from those of 105 controls.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urokinase; Bladder cancer; Single nucleotide polymorphism.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100003

Chromosome polymorphisms in natural populations of the South American grasshopper Sinipta dalmani
Provedor de dados: Genetics and Molecular Biology
Autores: Remis,Maria Isabel.
Six populations of Sinipta dalmani from the provinces of Buenos Aires and Entre Rios (Argentina) were analyzed. The populations of "El Palmar" National Park (Entre Rios) were polymorphic for pericentric inversions in pairs M4 and M7 and for a centric fusion involving pair M5 and the X chromosome. The M4 inversion remained similar over time and the karyomorphic frequencies did not depart from those expected according to the Hardy-Weinberg equilibrium. The analysis of chiasma frequency and distribution showed clear intra- and interchromosome effects of the different chromosome rearrangements. Both inversions and centric fusions were related with total or partial crossing over restriction in heterozygous condition, leading to a genetic differentiation between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pericentric inversion; Centric fusion; Chiasma conditions; Grasshopper.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100009

Bradyrhizobium elkanii nod regulon: insights through genomic analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Passaglia,Luciane M. P.
Abstract A successful symbiotic relationship between soybean [Glycine max (L.) Merr.] and Bradyrhizobium species requires expression of the bacterial structural nod genes that encode for the synthesis of lipochitooligosaccharide nodulation signal molecules, known as Nod factors (NFs). Bradyrhizobium diazoefficiens USDA 110 possesses a wide nodulation gene repertoire that allows NF assembly and modification, with transcription of the nodYABCSUIJnolMNOnodZ operon depending upon specific activators, i.e., products of regulatory nod genes that are responsive to signaling molecules such as flavonoid compounds exuded by host plant roots. Central to this regulatory circuit of nod gene expression are NodD proteins, members of the LysR-type regulator family. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bradyrhizobium; NodD1 protein; Nod box; Nod genes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400703

Quantitative real-time PCR for the clinical detection of Helicobacter pylori
Provedor de dados: Genetics and Molecular Biology
Autores: Ribeiro,Marcelo Lima; Ecclissato,Christina Cunha; Mattos,Ricardo Gabriel; Mendonca,Sergio; Pedrazzoli Jr.,José.
Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human gastric biopsy samples divided into three groups: 46 biopsies from untreated patients who according to the references methods were considered H. pylori-negative (group A); 35 biopsies from patients previously treated against H. pylori and considered to be cured by "gold standard" tests (group B); and 10 biopsies from patients H. pylori-positive by all available methods (group C). The sensitivity of the RT-PCR assay was higher than that of standard methods. Of the 81 patients considered to be uninfected according...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnostic methods; Helicobacter pylori; Real-time PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300022

Three novel α-L-iduronidase mutations in 10 unrelated Chinese mucopolysaccharidosis type I families
Provedor de dados: Genetics and Molecular Biology
Autores: Sun,Luning; Li,Chunyi; Song,Xiaoyu; Zheng,Ningning; Zhang,Haipeng; Dong,Guizhang.
Mucopolysaccharidosis type I (MPS I) arises from a deficiency in the α-L-iduronidase (IDUA) enzyme. Although the clinical spectrum in MPS I patients is continuous, it was possible to recognize 3 phenotypes reflecting the severity of symptoms, viz., the Hurler, Scheie and Hurler/Scheie syndromes. In this study, 10 unrelated Chinese MPS I families (nine Hurler and one Hurler/Scheie) were investigated, and 16 mutant alleles were identified. Three novel mutations in IDUA genes, one missense p.R363H (c.1088G &gt; A) and two splice-site mutations (c.1190-1G &gt; A and c.792+1G &gt; T), were found. Notably, 45% (nine out of 20) and 30% (six out of 20) of the mutant alleles in the 10 families studied were c.1190-1G &gt; A and c.792+1G &gt; T, respectively. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucopolysaccharidosis type I; Α-L-iduronidase; Mutation; Polymorphism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200004

Sugarcane expressed sequences tags (ESTs) encoding enzymes involved in lignin biosynthesis pathways
Provedor de dados: Genetics and Molecular Biology
Autores: Ramos,Rose Lucia Braz; Tovar,Francisco Javier; Junqueira,Ricardo Magrani; Lino,Fabiane Borges; Sachetto-Martins,Gilberto.
Lignins are phenolic polymers found in the secondary wall of plant conductive systems where they play an important role by reducing the permeability of the cell wall to water. Lignins are also responsible for the rigidity of the cell wall and are involved in mechanisms of resistance to pathogens. The metabolic routes and enzymes involved in synthesis of lignins have been largely characterized and representative genes that encode enzymes involved in these processes have been cloned from several plant species. The synthesis of lignins is liked to the general metabolism of the phenylpropanoids in plants, having enzymes (e.g. phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H) and caffeic acid O-methyltransferase (COMT)) common to other processes...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100031

The behavior of sympatric Chilean populations of Drosophila larvae during pupation
Provedor de dados: Genetics and Molecular Biology
Autores: Godoy-Herrera,Raúl; Silva-Cuadra,José Luis.
The behavioral mechanisms by which the larvae of sympatric Chilean populations of Drosophila melanogaster, D. simulans, D. hydei and D. busckii select pupation sites are described in terms of larval substrate preferences. These species share the same breeding sites in Central Valley of Chile. It is important to investigate larval substrate preferences to pupate in sympatric natural populations of species of Drosophila because such behavior could contribute to the coexistence of the species in the wild. D. busckii larvae preferred humid substrates with a smooth surface to pupate, whereas D. simulans larvae selected humid substrates with a rough surface. Larvae of D. melanogaster chose dry and humid substrates with a rough surface, whereas D. hydei larvae...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100007

Morphological variation in a secondary contact between divergent lineages of brown trout (Salmo trutta) from the Iberian Peninsula
Provedor de dados: Genetics and Molecular Biology
Autores: Hermida,Miguel; San Miguel,Eduardo; Bouza,Carmen; Castro,Jaime; Martínez,Paulino.
The aim of this study was to analyze the morphological variation of brown trout (Salmo trutta) in the Duero basin, an Atlantic river basin in the Iberian Peninsula, where a spatial segregation of two divergent lineages was previously reported, based on isozyme, microsatellite and mtDNA data. In these studies, two divergent pure regions (Pisuerga and Lower-course) and several hybrid populations between them were identified. Morphological variation was evaluated in 11 populations representative of the genetic differentiation previously observed in the Duero basin, using multivariate analysis on 12 morphometric and 4 meristic traits. A large differentiation between populations was observed (interpopulation component of variance: 41.8%), similar to that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brown trout; Secondary contact; Morphometric; Meristic; Multivariate analysis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100007

A multivariate approach to the association pattern of reciprocal translocations induced by chemicals and ionizing radiation in mouse germ cells
Provedor de dados: Genetics and Molecular Biology
Autores: De Luca,J.C.; Ortiz Jaureguizar,E.; Dulout,F.N.
The degree of similarity between chemical and physical agents in their capacity to induce reciprocal translocations was analyzed by means of multivariate analysis techniques. The effect of three different doses of gamma rays, four doses of X-rays and different doses of adriamycin, mitomycin C, thio-tepa and bleomycin was analyzed. Data were arranged in a basic matrix by two methods: cluster analysis and ordination. Two main groups were found, one including doses of 9 and 10 Gy and the other including the remaining lower doses of ionizing radiation and the other chemicals. Various subgroups were found within the second group. Accordingly, using presence/absence data there was not a specific pattern of chromosomal damage induction for physical and chemical...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300014

Prevalence of α-thalassemia 3.7 kb deletion in the adult population of Rio Grande do Norte, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Alcoforado,Gustavo Henrique de Medeiros; Bezerra,Christiane Medeiros; Lemos,Telma Maria Araújo Moura; Oliveira,Denise Madureira de; Kimura,Elza Miyuki; Costa,Fernando Ferreira; Sonati,Maria de Fátima; Medeiros,Tereza Maria Dantas de.
α-Thalassemia, arising from a defect in a-globin chain synthesis, is often caused by deletions involving one or both of the a-genes on the same allele. With the aim of investigating the prevalence of α-thalassemia 3.7 kb deletion in the adult population of Rio Grande do Norte, 713 unrelated individuals, between 18 and 59 years-of-age, were analyzed. Red blood cell indices were electronically determined, and A2 and F hemoglobins evaluated by HPLC. PCR was applied to the molecular investigation of α-thalassemia 3.7 kb deletion. Eighty (11.2%) of the 713 individuals investigated presented α-thalassemia, of which 79 (11.1%) were heterozygous (-α3.7/αα) deletions and 1 (0.1%) homozy- gous (-α3.7/-α3.7). Ethnically, heterozygous deletions were higher (24.8%) in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-thalassemia; -α3.7 kb deletion; Brazilian population.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400008

Differential survivorship of congeneric ornamental fishes under forecasted climate changes are related to anaerobic potential
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Luciana Mara Fé; Silva,Maria de Nazaré Paula da; Val,Adalberto Luis; Almeida-Val,Vera Maria Fonseca de.
Abstract Two Amazonian closely related tetras – cardinal Paracheirodon axelrodi and green neon P. simulans – were artificially acclimatized to environmental chambers mimicking future climate change scenarios (mild, moderate and extreme), using a microcosm facility. P. simulans survived (100%) to all scenarios after 30 days exposure, while P. axelrodi presented decreasing survival percentages according to environmental severity. These differences may be the reflection of distinct natural acclimatization to microhabitats between the species, which differ in thermal conditions. Survival responses might be related to differences in relative gene expression of lactate dehydrogenase (Ldh), suggesting that P. axelrodi anaerobic potential is lower or non-existent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ornamental fish; Enzyme activity; Relative gene expression; IPCC scenarios; Lactate Dehydrogenase (Ldh).
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100107

A simple procedure for rehybridization of nuclei analyzed previously by fish
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho-Salles,Andréa Borduchi; Barril,Nilce; Tajara,Eloiza Helena.
We present a simple procedure for interphase fluorescence in situ rehybridization (FISH). This procedure was used to evaluate fresh prostate tumor from needle biopsy specimens. Digoxigenin-labeled DNA probes were hybridized onto nuclei which had been previously investigated by FISH using biotin-labeled DNA probes. This method makes it possible to reanalyze the same nuclei with different centromeric DNA probes and is useful in cases where a limited number of slides are available.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200006

Chromosome characterization in Thinopyrum ponticum (Triticeae, Poaceae) using in situ hybridization with different DNA sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Brasileiro-Vidal,Ana Christina; Cuadrado,Angeles; Brammer,Sandra P.; Zanatta,Ana Christina A.; Prestes,Ariano M.; Moraes-Fernandes,Maria Irene B.; Guerra,Marcelo.
Thinopyrum ponticum (2n = 10x = 70, JJJJsJs) belongs to the Triticeae tribe, and is currently used as a source of pathogen resistance genes in wheat breeding. In order to characterize its chromosomes, the number and position of 45S and 5S rDNA sites, as well as the distribution of the repetitive DNA sequences pAs1 and pSc119.2, were identified by fluorescent in situ hybridization. The number of nucleoli and NORs was also recorded after silver nitrate staining. Seventeen 45S and twenty 5S rDNA sites were observed on the short arms of 17 chromosomes, the 45S rDNA was always located terminally. On three other chromosomes, only the 5S rDNA site was observed. Silver staining revealed a high number of Ag-NORs (14 to 17) on metaphase chromosomes, whereas on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thinopyrum; Silver staining; 45S and 5S rDNA; PSc119.2; PAs1.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400014

Trichoderma harzianum transformant has high extracellular alkaline proteinase expression during specific mycoparasitic interactions
Provedor de dados: Genetics and Molecular Biology
Autores: Goldman,Maria Helena S.; Goldman,Gustavo H.
The mycoparasite Trichoderma harzianum produces an alkaline proteinase that may be specifically involved in mycoparasitism. We have constructed transformant strains of this fungus that overexpress this alkaline proteinase. Some of the transformants were assessed for alkaline proteinase activity, and those with higher activity than the wild type were selected for further studies. One of these transformant strains produced an elevated and constitutive pbr1 mRNA level during mycoparasitic interactions with Rhizoctonia solani.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300007

Contribution of WUSCHEL-related homeobox (WOX) genes to identify the phylogenetic relationships among Petunia species
Provedor de dados: Genetics and Molecular Biology
Autores: Segatto,Ana Lúcia Anversa; Thompson,Claudia Elizabeth; Freitas,Loreta Brandão.
Abstract Developmental genes are believed to contribute to major changes during plant evolution, from infrageneric to higher levels. Due to their putative high sequence conservation, developmental genes are rarely used as molecular markers, and few studies including these sequences at low taxonomic levels exist. WUSCHEL-related homeobox genes (WOX) are transcription factors exclusively present in plants and are involved in developmental processes. In this study, we characterized the infrageneric genetic variation of Petunia WOX genes. We obtained phylogenetic relationships consistent with other phylogenies based on nuclear markers, but with higher statistical support, resolution in terminals, and compatibility with flower morphological changes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular phylogeny; Recently diverged species; Developmental genes.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400658

Putative resistance genes in the CitEST database
Provedor de dados: Genetics and Molecular Biology
Autores: Guidetti-Gonzalez,Simone; Carrer,Helaine.
Disease resistance in plants is usually associated with the activation of a wide variety of defense responses to prevent pathogen replication and/or movement. The ability of the host plant to recognize the pathogen and to activate defense responses is regulated by direct or indirect interaction between the products of plant resistance (R) and pathogen avirulence (Avr) genes. Attempted infection of plants by avirulent pathogens elicits a battery of defenses often followed by the collapse of the challenged host cells. Localized host cell death may help to prevent the pathogen from spreading to uninfected tissues, known as hypersensitive response (HR). When either the plant or the pathogen lacks its cognate gene, activation of the plant’s defense responses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypersensitive response (HR); Plant disease resistance; Citrus; EST sequences.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500021

Micronuclei formation in liver fibrosis samples from patients infected by hepatitis C virus
Provedor de dados: Genetics and Molecular Biology
Autores: Almeida,Terezinha M.B. de; Leitão,Regina Maria C.; Carrilho,Flair J.; Sonohara,Shigueko.
Genetic research on fibrosis outset and its progression in chronic hepatitis (CH) by hepatitis C virus (HCV) are limited. The lack of cytogenetic data led us to investigate the presence of micronuclei (MNi), as a sign of genomic damage. Hepatocytes of hepatic parenchyma from 62 cases diagnosed with CH associated with HCV and displaying different degrees of fibrosis (F1-F4) were analyzed. These data were compared to 15 cases without fibrosis (F0). Twelve healthy liver parenchyma samples were included as control. All samples were obtained from paraffin-embedded archival material. Micronucleated hepatocytes (MN-Heps) were analyzed through Feulgen/Fastgreen staining. Results showed that the rates of MN-Heps in the F4 group were statistically significant (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronuclei; Hepatocytes; Chronic hepatitis C; Fibrosis progression; HCV-patients.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300003

Ant-Based Phylogenetic Reconstruction (ABPR): A new distance algorithm for phylogenetic estimation based on ant colony optimization
Provedor de dados: Genetics and Molecular Biology
Autores: Vittori,Karla; Delbem,Alexandre C.B.; Pereira,Sérgio L.
We propose a new distance algorithm for phylogenetic estimation based on Ant Colony Optimization (ACO), named Ant-Based Phylogenetic Reconstruction (ABPR). ABPR joins two taxa iteratively based on evolutionary distance among sequences, while also accounting for the quality of the phylogenetic tree built according to the total length of the tree. Similar to optimization algorithms for phylogenetic estimation, the algorithm allows exploration of a larger set of nearly optimal solutions. We applied the algorithm to four empirical data sets of mitochondrial DNA ranging from 12 to 186 sequences, and from 898 to 16,608 base pairs, and covering taxonomic levels from populations to orders. We show that ABPR performs better than the commonly used Neighbor-Joining...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogenetic estimation; Distance algorithms; Ant colony optimization.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500027

A novel G21R mutation of the GJB2 gene causes autosomal dominant non-syndromic congenital deafness in a Cuban family
Provedor de dados: Genetics and Molecular Biology
Autores: Rabionet,Raquel; Morales-Peralta,Estela; López-Bigas,Núria; Arbonés,Maria Lourdes; Estivill,Xavier.
Deafness is a complex disorder affecting 1/1000 infants. In developed countries, more than 50% of deafness cases are thought to have a genetic cause. At least 40 loci for dominant non-syndromic deafness and another 30 for recessive non-syndromic deafness have been described. Mutations in the GJB2 gene are the cause of an important number of cases of non-syndromic recessive deafness but are not as common in non-syndromic dominant deafness cases. We describe here a new dominant mutation (G21R) in the GJB2 gene which causes deafness and has been identified in a three generation Cuban family with dominant non-syndromic congenital sensorineural profound deafness.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Connexin 26; GJB2; DFNA3; Hearing impairment.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300006

Bayesian inference in genetic parameter estimation of visual scores in Nellore beef-cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Faria,Carina Ubirajara de; Koury Filho,William; Magnabosco,Cláudio Ulhôa; Albuquerque,Lúcia Galvão de; Bezerra,Luiz Antônio Framartino; Lôbo,Raysildo Barbosa.
The aim of this study was to estimate the components of variance and genetic parameters for the visual scores which constitute the Morphological Evaluation System (MES), such as body structure (S), precocity (P) and musculature (M) in Nellore beef-cattle at the weaning and yearling stages, by using threshold Bayesian models. The information used for this was gleaned from visual scores of 5,407 animals evaluated at the weaning and 2,649 at the yearling stages. The genetic parameters for visual score traits were estimated through two-trait analysis, using the threshold animal model, with Bayesian statistics methodology and MTGSAM (Multiple Trait Gibbs Sampler for Animal Models) threshold software. Heritability estimates for S, P and M were 0.68, 0.65 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphological traits; Threshold model; Genetic parameters; Nellore.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400015

Heterogeneous ethnic distribution of the factor v leiden mutation
Provedor de dados: Genetics and Molecular Biology
Autores: Franco,Rendrik F.; Elion,Jacques; Santos,Sidney E.B.; Araújo,Amélia G.; Tavella,Marli H.; Zago,Marco A.
Inherited resistance to activated protein C caused by the factor V Leiden (FVL) mutation is the most common genetic cause of venous thrombosis yet described, being found in 20-60% of patients with venous thrombophilia. A relationship between the FVL mutation and an increased predisposition to arterial thrombosis in young women was recently reported. We assessed the prevalence of the FVL mutation in 440 individuals (880 chromosomes) belonging to four different ethnic groups: Caucasians, African Blacks, Asians and Amerindians. PCR amplification followed by MnlI digestion was employed to define the genotype. The FVL mutation was found in a heterozygous state in four out of 152 Whites (2.6%), one out of 151 Amerindians (0.6%), and was absent among 97 African...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200001

CCR5D32 mutation in three Brazilian populations of predominantly Sub-Saharan African ancestry
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Mônica W.P.; Leboute,Ana P.M.; Oliveira,Silviene F.; Sousa,Sandra M.B.; Klautau-Guimarães,Maria de Nazaré; Simões,Aguinaldo L.
This study reports the frequencies of the CCR5D32 mutation of the beta-chemokine 5 gene and discusses the possible effects of past and recent gene flow in three quilombo remnants (Brazilians communities with anthropological African ancestry whose ancestors were escaped slaves): Rio das Rãs, Mocambo, and São Gonçalo in the northeastern region of Brazil. The CCR5D32 allele frequency of the Mocambo population was significantly higher (5.6%) than that found in the Rio das Rãs (1%) and São Gonçalo (0.9%) populations. These differences may reflect different proportions of parental populations in the founders individuals, a founder-effect and/or different histories of inter-ethnic contact. The frequency of the CCR5D32 allele in the Mocambo sample is similar to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CCR5; Genetic polymorphism; Sub-Saharan Afro-derived Brazilian populations; Ethnic-grouspecific marker.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300002

The MTHFR C677T polymorphism and global DNA methylation in oral epithelial cells
Provedor de dados: Genetics and Molecular Biology
Autores: Arruda,Isabela Tatiana Sales de; Persuhn,Darlene Camati; Oliveira,Naila Francis Paulo de.
DNA methylation is mediated by DNA methyltransferases (DNMTs) that add a methyl group to the 5'-carbon of cytosine. The enzyme methylenetetrahydrofolate reductase (MTHFR) catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the rate-limiting step of the cycle involving the methyl donor S-adenosyl-L-methionine (SAM). The MTHFR C677T polymorphism results in a thermolabile enzyme with reduced activity that is predicted to influence the DNA methylation status. In this study, we investigated the impact of the MTHFR C677T polymorphism on the global DNA methylation of oral epithelial cells obtained from 54 healthy subjects. There were no significant differences in global DNA methylation among the MTHFR CC, CT and TT genotypes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA methylation; Epigenetic; MTHFR C677T; Oral epithelial cells; Polymorphism.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400004

Immediate response of myocardium to pressure overload includes transient regulation of genes associated with mitochondrial bioenergetics and calcium availability
Provedor de dados: Genetics and Molecular Biology
Autores: Deckmann,Ana Carolina; Theizen,Thaís Holz; Medrano,Francisco Javier; Franchini,Kleber Gomes; Pereira,Gonçalo Amarante Guimarães.
Ventricular hypertrophy is one of the major myocardial responses to pressure overload (PO). Most studies on early myocardial response focus on the days or even weeks after induction of hypertrophic stimuli. Since mechanotransduction pathways are immediately activated in hearts undergoing increased work load, it is reasonable to infer that the myocardial gene program may be regulated in the first few hours. In the present study, we monitored the expression of some genes previously described in the context of myocardial hypertrophic growth by using the Northern blot technique, to estimate the mRNA content of selected genes in rat myocardium for the periods 1, 3, 6, 12 and 48 h after PO stimuli. Results revealed an immediate switch in the expression of genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pressure overload; Myocardial hypertrophy; Gene expression; SERCA2.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100004

Mapping QTLs for drought tolerance in a SEA 5 x AND 277 common bean cross with SSRs and SNP markers
Provedor de dados: Genetics and Molecular Biology
Autores: Briñez,Boris; Perseguini,Juliana Morini Küpper Cardoso; Rosa,Juliana Santa; Bassi,Denis; Gonçalves,João Guilherme Ribeiro; Almeida,Caléo; Paulino,Jean Fausto de Carvalho; Blair,Matthew Ward; Chioratto,Alisson Fernando; Carbonell,Sérgio Augusto Morais; Valdisser,Paula Arielle Mendes Ribeiro; Vianello,Rosana Pereira; Benchimol-Reis,Luciana Lasry.
Abstract The common bean is characterized by high sensitivity to drought and low productivity. Breeding for drought resistance in this species involves genes of different genetic groups. In this work, we used a SEA 5 x AND 277 cross to map quantitative trait loci associated with drought tolerance in order to assess the factors that determine the magnitude of drought response in common beans. A total of 438 polymorphic markers were used to genotype the F8 mapping population. Phenotyping was done in two greenhouses, one used to simulate drought and the other to simulate irrigated conditions. Fourteen traits associated with drought tolerance were measured to identify the quantitative trait loci (QTLs). The map was constructed with 331 markers that covered all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic stress; Interpopulation gene-pool; Molecular markers; QTL mapping; Water deficit.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500813

Patterns of molecular evolution in pathogenesis-related proteins
Provedor de dados: Genetics and Molecular Biology
Autores: Scherer,Nicole M.; Thompson,Claudia E.; Freitas,Loreta B.; Bonatto,Sandro L.; Salzano,Francisco M.
The genes encoding 13 classes of pathogenesis-related (PR) proteins were examined for positive selection using maximum-likelihood (ML) models of codon substitution. The study involved 194 sequences from 54 species belonging to 37 genera. Although the sizes of the sequences examined varied from 237 bp for PR12 to 1,110 bp for PR7, most classes (9 out of 13) contained sequences made up of more than 400 nucleotides. Signs of positive selection were obtained for sites in PR proteins 4, 6, 8, 9 and 15 using an ML-based Bayesian method and likelihood ratio tests. These results confirm the importance of positive selection in proteins related to defense mechanisms already observed in a wide array of organisms.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogenesis-related proteins; PRs; Molecular variability; Positive selection; Maximum-likelihood methods.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500001

Wavelet-domain elastic net for clustering on genomes strains
Provedor de dados: Genetics and Molecular Biology
Autores: Ferreira,Leila Maria; Sáfadi,Thelma; Ferreira,Juliano Lino.
Abstract We propose to evaluate genome similarity by combining discrete non-decimated wavelet transform (NDWT) and elastic net. The wavelets represent a signal with levels of detail, that is, hidden components are detected by means of the decomposition of this signal, where each level provides a different characteristic. The main feature of the elastic net is the grouping of correlated variables where the number of predictors is greater than the number of observations. The combination of these two methodologies applied in the clustering analysis of the Mycobacterium tuberculosis genome strains proved very effective, being able to identify clusters at each level of decomposition.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elastic net; Genome; GC-content; Cluster analysis; Wavelet transform.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500884

Association between HPV infection and prostate cancer in a Mexican population
Provedor de dados: Genetics and Molecular Biology
Autores: Medel-Flores,Olivia; Valenzuela-Rodríguez,Vania Alejandra; Ocadiz-Delgado,Rodolfo; Castro-Muñoz,Leonardo Josué; Hernández-Leyva,Sandra; Lara-Hernández,Gabriel; Silva-Escobedo,Jesús-Gabriel; Vidal,Patricio Gariglio; Sánchez-Monroy,Virginia.
Abstract The aim of this study was to evaluate the association between prostate cancer (PCa) and Human papillomavirus (HPV) infection in the Mexican population. We studied 356 paraffin-embedded tissues from unrelated Mexican men with PCa or benign prostatic hyperplasia (BPH), with the latter serving as control. HPV detection was performed by polymerase chain reaction (PCR) using universal primers, and viral genotypes were detected using sequencing or multiplex PCR. Light microscopy analyses enabled the identification of koilocytes in samples subsequently analyzed for HPV detection by in situ PCR and for p16-INK4A expression by immunohistochemistry. The results showed that high risk- (HR) HPVs were detected in 37/189 (19.6%) PCa specimens compared to 16/167...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV; Prostate; Cancer; Koilocytes.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500781

Evo-devo of non-bilaterian animals
Provedor de dados: Genetics and Molecular Biology
Autores: Lanna,Emilio.
The non-bilaterian animals comprise organisms in the phyla Porifera, Cnidaria, Ctenophora and Placozoa. These early-diverging phyla are pivotal to understanding the evolution of bilaterian animals. After the exponential increase in research in evolutionary development (evo-devo) in the last two decades, these organisms are again in the spotlight of evolutionary biology. In this work, I briefly review some aspects of the developmental biology of nonbilaterians that contribute to understanding the evolution of development and of the metazoans. The evolution of the developmental genetic toolkit, embryonic polarization, the origin of gastrulation and mesodermal cells, and the origin of neural cells are discussed. The possibility that germline and stem cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Early-diverging metazoans; BMP; FGF; Wnt; Stem cells; Evolution; Nervous system.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300284

The golden age of Drosophila research at the Universidade de São Paulo (USP): a testimonial on the decades 1940-1950
Provedor de dados: Genetics and Molecular Biology
Autores: Magalhães,Luiz Edmundo de; Vilela,Carlos Ribeiro.
This article is a testimonial written by the first author regarding the research work performed with Drosophila between 1943 and 1959, at the Departamento de Biologia Geral of the Universidade de São Paulo (USP), which permitted the building of a nucleus of excellence. This research work, focused on the systematics and population genetics of the Neotropical species, began during the first of Dobzhansky's six visits to USP. Special attention was given to the multinational megaprojects conducted during his longer stays, from August 1948 to July 1949 and from June 1955 to July 1956. The role played by the Rockefeller Foundation is duly remembered, and so is the undeniable contribution brought by Dobzhansky, to the establishment of several laboratories...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Departamento de Biologia Geral; FFCL; Rockefeller Foundation; Population genetics; Theodosius Dobzhansky.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100020

A brief history of fish genetics in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Foresti,Fausto.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200038

Phenotypic recurrent selection in the common bean (Phaseolus vulgaris L) with carioca-type grains for resistance to the fungi Phaeoisariopsis griseola
Provedor de dados: Genetics and Molecular Biology
Autores: Amaro,Geovani Bernardo; Abreu,Ângela de Fátima Barbosa; Ramalho,Magno Antonio Patto; Silva,Flávia Barbosa.
The efficiency of recurrent selection was assessed in obtaining common bean (Phaseolus vulgaris L) plant lines resistant to the phytopathogenic fungi Phaeoisariopsis griseola. The base bean population was obtained from the partial diallel between seven lines with carioca-type grains and 10 sources of resistance to P. griseola. The plants most resistant to the pathogen were selected in the F2 (S0) generation of the populations (C-0). The best S0:1 plants that presented carioca-type grains were intercrossed to obtain cycle I (C-I). The same procedure was adopted to obtain cycles C-II to C-V. In each recurrent selection cycle, S0:1 progenies selected were also assessed in experiments carried out in Lavras, Brazil, always using as check the Carioca MG...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angular-leaf-spot; Grain yield; Heritability; Phaseolus vulgaris; Response to selection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400014

Estimating the effects of population size and type on the accuracy of genetic maps
Provedor de dados: Genetics and Molecular Biology
Autores: Ferreira,Adésio; Silva,Marcia Flores da; Silva,Luciano da Costa e; Cruz,Cosme Damião.
Based on simulation studies, it was shown that the type and size of experimental populations can exert an influence on the accuracy of genetic maps. A hypothetical genome map (one chromosome with nine equidistant molecular markers) was generated for the following population types: F2 with dominant and co-dominant markers, backcrossing, recombinant inbred lines (RIL) and double-haploid. The population sizes were 50, 100, 150, 200, 500 and 1000 individuals and 100 simulations were made for each population. The inaccuracies of the populations with the lowest number of individuals were shown by inversions in the order of the markers and the establishment of more than one linkage group in up to 38% of the simulations, depending on the population type. Stress...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental populations; Mapping; Simulation; Markers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100033

In silico analysis of the endogenous time-keeping mechanism in citrus
Provedor de dados: Genetics and Molecular Biology
Autores: Quecini,Vera.
The endogenous time-keeping mechanism is responsible for organizing plant physiology and metabolism according to periodic environmental changes, such as diurnal cycles of light and dark and seasonal progression throughout the year. In plants, circadian rhythms control gene expression, stomatal opening, and the timing component of the photoperiodic responses, leading to enhanced fitness due to increased photosynthetic rates and biomass production. We have investigated the citrus genome databases of expressed sequence tags (EST) in order to identify genes coding for functionally characterized proteins involved in the endogenous time-keeping mechanism in Arabidopsis thaliana. Approximately 180,000 EST sequences from 53 libraries were investigated and 81...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central oscillator; Circadian clock; Data mining; Photoperiodic responses; Regulatory feedback loop.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500008

Proteomic analysis of a clavata-like phenotype mutant in Brassica napus
Provedor de dados: Genetics and Molecular Biology
Autores: Zhu,Keming; Zhang,Weiwei; Sarwa,Rehman; Xu,Shuo; Li,Kaixia; Yang,Yanhua; Li,Yulong; Wang,Zheng; Cao,Jun; Li,Yaoming; Tan,Xiaoli.
Abstract Rapeseed is one of important oil crops in China. Better understanding of the regulation network of main agronomic traits of rapeseed could improve the yielding of rapeseed. In this study, we obtained an influrescence mutant that showed a fusion phenotype, similar with the Arabidopsis clavata-like phenotype, so we named the mutant as Bnclavata-like (Bnclv-like). Phenotype analysis illustrated that abnormal development of the inflorescence meristem (IM) led to the fused-inflorescence phenotype. At the stage of protein abundance, major regulators in metabolic processes, ROS metabolism, and cytoskeleton formation were seen to be altered in this mutant. These results not only revealed the relationship between biological processes and inflorescence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brassica napus; Proteomic; Inflorescence meristem (IM); Bnclavata-like (Bnclv-like); Quantitative real-time PCR.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100308

Apoptosis in Trypanosomatids: Evolutionary and phylogenetic considerations
Provedor de dados: Genetics and Molecular Biology
Autores: Barcinski,Marcello A.
Programmed cell death (PCD) or apoptosis, an active process of cell death, plays a central role in normal tissue development and organogenesis, as well as in the pathogenesis of different diseases. Although it occurs in diverse cells and tissues under the influence of a remarkable variety of inducing agents, the resultant ultrastructural and biochemical changes are extremely monotonous, indicating the existence of a common biological mechanism underlying its occurrence. It is generally accepted that a developmental program leading to cell death cannot be advantageous to unicellular organisms and that PCD appeared in evolution to fulfill the organizational needs of multicellular life. However, the recent description of apoptotic death occurring in three...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100005

Birth defects in Brazil: Outcomes of a population-based study
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira-Brancati,Camila Ive Ferreira; Ferrarese,Valéria Cristina Carvalho; Costa,Antonio Richieri; Fett-Conte,Agnes Cristina.
Abstract Birth defects (BDs) are functional and structural alterations in embryonic or fetal development. With an incidence of approximately 3-5%, BDs are a leading cause of infant mortality and lifelong disability. A population-based prospective case-control study was conducted for one year with 5204 infants, between March 1st, 2011 and February 29th, 2012 in the city of São José do Rio Preto, State of São Paulo, Brazil. The incidence of BDs was 3.2% [95% confidence interval (95%CI): 2.8-3.8%]. The most common congenital anomalies were heart diseases in isolation (11.2%; 95%CI: 7.3-16.9%) followed by Down syndrome (9.5%; 95%CI: 5.9-14.8%), neural tube defects (8.9%; 95%CI: 5.4-14.1), urinary tract anomalies (7.7%; 95%CI: 4.4-12.7%), and polydactyly (7.0%;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malformation; Congenital anomaly; Fetal development; Genetic counseling; Public health.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100102

Comparative cytogenetic analysis in the species Uroderma magnirostrum and U. bilobatum (cytotype 2n = 42) (Phyllostomidae, Stenodermatinae) in the Brazilian Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Adailton Moreira da; Marques-Aguiar,Suely Aparecida; Barros,Regina Maria de Souza; Nagamachi,Cleusa Yoshiko; Pieczarka,Julio Cesar.
The genus Uroderma includes two species: U. magnirostrum and U. bilobatum. These species are characterized by their high degree of karyotypic evolution, diverging from most other species of the subfamily Stenodermatinae, which have a lower degree of chromosomic evolution. The present study reports the first banding patterns of U. magnirostrum (G-, C-banding and Ag-NOR) and U. bilobatum (C-banding and Ag-NOR). The chromosomic data in conventional staining of U. magnirostrum (2n = 36, NF = 62) and U. bilobatum (cytotype 2n = 42, NF = 50) are equivalent to that described in the literature. When compared, chromosomal homeologies are found in both karyotypes, as well as differences, confirming that karyotypic evolution in the Uroderma genus is intense. Fission,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Chromosomal evolution; Phyllostomidae; Uroderma.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200012

Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A. m. lamarckii
Provedor de dados: Genetics and Molecular Biology
Autores: Kandemir,Irfan; Pinto,Maria A.; Meixner,Marina D.; Sheppard,Walter S.
Restriction fragment length polymorphism of whole mitochondrial DNA or PCR amplified mtDNA regions are known to be useful in discriminating among honey bee lineages and also some individual subspecies. In this study, PCR-amplified fragments of cytochrome oxidase I (CO-I) and cytochrome B (Cyt B) of honey bees sampled from different countries (Cyprus, Turkey, Ethiopia, Syria and Egypt) were digested with Hinf I and Bgl II restriction enzymes, respectively. Eastern Europe and Mediterranean honey bee subspecies were separated by the Cyt B/Bgl II analysis, although Hinf I digestion of the CO-I region yielded much finer resolution within different honey bee lineages. Here we report that CO-I/Hinf-I is a discriminative test for the mitochondrial "O" lineage,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; Cytochrome oxidase I; Apis mellifera lamarckii; O lineage.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400027

Isoenzyme variation in the leaf-cutting ants Acromyrmex heyeri and Acromyrmex striatus (Hymenoptera, formicidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Diehl,Elena; Cavalli-Molina,Suzana; Araújo,Aldo Mellender de.
This is the first study of isoenzyme variability in the leaf-cutting ants (Myrmicinae, Attini) Acromyrmex heyeri (Forel, 1899) and A. striatus (Roger, 1863) which are common throughout the southern Brazilian state of Rio Grande do Sul. We studied the alloenzyme variability of malate dehydrogenase (MDH), alpha-glycerophosphate dehydrogenase (alpha-GPDH) and amylase (AMY) in 97 colonies of A. heyeri and 103 colonies of A. striatus. Five loci were found for these enzyme systems, one locus (Amy-1) being monomorphic in both species and four loci (Mdh-1, alpha-Gpdh-1, Amy-2, and Amy-4) being polymorphic. For each species there were exclusive alleles for the Mdh-1 and Amy-2 loci and differences were also found in the allele frequencies for the other polymorphic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ants; Allozyme variation; Fungus grower ants; Genetic polymorphism; Haplodiploid.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200010

Dealing with iron metabolism in rice: from breeding for stress tolerance to biofortification
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Railson Schreinert dos; Araujo Júnior,Artur Teixeira de; Pegoraro,Camila; Oliveira,Antonio Costa de.
Abstract Iron is a well-known metal. Used by humankind since ancient times in many different ways, this element is present in all living organisms, where, unfortunately, it represents a two-way problem. Being an essential block in the composition of different proteins and metabolic pathways, iron is a vital component for animals and plants. That is why iron deficiency has a severe impact on the lives of different organisms, including humans, becoming a major concern, especially in developing countries where access to adequate nutrition is still difficult. On the other hand, this metal is also capable of causing damage when present in excess, becoming toxic to cells and affecting the whole organism. Because of its importance, iron absorption, transport and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iron toxicity; Mineral malnutrition; Fe-enrichment; Quantitative Trait Loci.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200312

DNA repair genes XRCC1 and XRCC3 polymorphisms and their relationship with the level of micronuclei in breast cancer patients
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Raquel A.; Teixeira,Ana Claudia; Mayorano,Monica B.; Carrara,Helio H.A.; Andrade,Jurandyr M.; Takahashi,Catarina S.
Breast cancer (BC) is the most prevalent type worldwide, besides being one of the most common causes of death among women. It has been suggested that sporadic BC is most likely caused by low-penetrance genes, including those involved in DNA repair mechanisms. Furthermore, the accumulation of DNA damage may contribute to breast carcinogenesis. In the present study, the relationship between two DNA repair genes, viz., XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) polymorphisms, and the levels of chromosome damage detected in 65 untreated BC women and 85 healthy controls, was investigated. Chromosome damage was evaluated through micronucleus assaying, and genotypes determined by PCR-RFLP methodology. The results showed no alteration in the risk of BC and DNA damage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA repair polymorphisms; Breast cancer; Micronucleus assay.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400007

Spatial and temporal population genetic variation and structure of Nothotsuga longibracteata (Pinaceae), a relic conifer species endemic to subtropical China
Provedor de dados: Genetics and Molecular Biology
Autores: Qiu,Yingjun; Liu,Yifei; Kang,Ming; Yi,Guanmei; Huang,Hongwen.
Nothotsuga longibracteata, a relic and endangered conifer species endemic to subtropical China, was studied for examining the spatial-temporal population genetic variation and structure to understand the historical biogeographical processes underlying the present geographical distribution. Ten populations were sampled over the entire natural range of the species for spatial analysis, while three key populations with large population sizes and varied age structure were selected for temporal analyses using both nuclear microsatellites (nSSR) and chloroplast microsatellites (cpSSR). A recent bottleneck was detected in the natural populations of N. longibracteata. The spatial genetic analysis showed significant population genetic differentiation across its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene flow; Glacial refugia; Nothotsuga longibracteata; Spatial genetic structure; Temporal genetic structure.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400019

Nucleotide sequence, genomic organization and chromosome localization of 5S rDNA in two species of Curimatidae (Teleostei, Characiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Lessandra Viviane de Rosa; Foresti,Fausto; Wasko,Adriane Pinto; Oliveira,Claudio; Martins,Cesar.
The 5S ribosomal DNA (5S rDNA) of higher eukaryotes is organized in repeat units of tandem arrays composed of a 5S rDNA coding region, conserved even among non-related taxa, and a variable non-transcribed spacer sequence (NTS). To contribute to knowledge on the organization and evolution of vertebrate 5S rDNA we used PCR, nucleotide sequencing, Southern blot hybridization and chromosome fluorescence in situ hybridization (FISH) to investigate 5S rDNA tandem repeats in the South American Curimatidae fish Steindachnerina insculpta and Cyphocharax modesta. 5S rDNA repeats of 180 base pairs (bp) from both species were PCR-generated and sequenced evidencing the shortest 5S rDNA monomer so far described in eukaryote species. Southern blotting revealed that both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyphocharax modesta; Steindachnerina insculpta; 5S rDNA; Chromosomes; FISH; Nucleotide sequence.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200009

Oswaldo Frota-Pessoa (1917-2010): a successful three-lane road in science
Provedor de dados: Genetics and Molecular Biology
Autores: Vianna-Morgante,Angela M.; Otto,Paulo A.
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300001

Analysis of topological organization of chromatin during spermatogenesis in mouse testis
Provedor de dados: Genetics and Molecular Biology
Autores: Narayan,Gopeshwar; Raman,Rajiva.
Eukaryotic chromatin is organized as radial DNA loops with periodical attachments to an underlying nucleoskeleton known as nuclear matrix. This higher order chromatin organization is revealed upon high salt extraction of cells. To understand the sequential change in the functional organization of chromatin during spermatogenesis, we have analysed the higher order organization of chromatin in different testicular cell types and the epididymal sperm of laboratory mouse. The expansion and contraction of the nucleoid DNA following 2 M NaCl extraction was measured in a fluorescence microscope using ethidium bromide (2.5-200 mg/mL) as an intercalating dye to induce DNA positive supercoils. While the halo size varied among cell types (pachytene DNA most extended,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromatin; DNase I; Nucleoid; Spermatogenesis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100006

Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
Provedor de dados: Genetics and Molecular Biology
Autores: Zaros,Lilian Giotto; Bricarello,Patrízia Ana; Amarante,Alessandro Francisco Talamini; Coutinho,Luiz Lehmann.
T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytokine gene expression; Real-time RT-PCR; Cattle; Gastrointestinal nematodes; Bovine cytokines.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012

Genome-wide association study of pre-harvest sprouting resistance in Chinese wheat founder parents
Provedor de dados: Genetics and Molecular Biology
Autores: Lin,Yu; Liu,Shihang; Liu,Yaxi; Liu,Yujiao; Chen,Guoyue; Xu,Jie; Deng,Mei; Jiang,Qiantao; Wei,Yuming; Lu,Yanli; Zheng,Youliang.
Abstract Pre-harvest sprouting (PHS) is a major abiotic factor affecting grain weight and quality, and is caused by an early break in seed dormancy. Association mapping (AM) is used to detect correlations between phenotypes and genotypes based on linkage disequilibrium (LD) in wheat breeding programs. We evaluated seed dormancy in 80 Chinese wheat founder parents in five environments and performed a genome-wide association study using 6,057 markers, including 93 simple sequence repeat (SSR), 1,472 diversity array technology (DArT), and 4,492 single nucleotide polymorphism (SNP) markers. The general linear model (GLM) and the mixed linear model (MLM) were used in this study, and two significant markers (tPt-7980 and wPt-6457) were identified. Both markers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: General linear model; Linkage disequilibrium; Marker-trait associations; Mixed linear model; Seed dormancy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400620

Orthogonal projections and bootstrap resampling procedures in the study of infraspecific variation
Provedor de dados: Genetics and Molecular Biology
Autores: Duarte,Luiza Carla; Von Zuben,Fernando José; Reis,Sérgio Furtado dos.
The effect of an increase in quantitative continuous characters resulting from indeterminate growth upon the analysis of population differentiation was investigated using, as an example, a set of continuous characters measured as distance variables in 10 populations of a rodent species. The data before and after correction for allometric size effects using orthogonal projections were analyzed with a parametric bootstrap resampling procedure applied to canonical variate analysis. The variance component of the distance measures attributable to indeterminate growth within the populations was found to be substantial, although the ordination of the populations was not affected, as evidenced by the relative and absolute positions of the centroids. The covariance...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400013

Perinatal mortality among twins and singletons in a city in southeastern Brazil, 1984-1996
Provedor de dados: Genetics and Molecular Biology
Autores: Beiguelman,Bernardo; Franchi-Pinto,Carla.
Analysis of 116,699 deliveries (1062 twin pairs and 115,637 singletons) which occurred from 1984 to 1996 at the largest obstetric center in Campinas, SP, Brazil, provided the following conclusions: 1) despite the low percentage of twin births (0.9%), 10.7% of all early neonatal deaths and 3.5% of all stillbirths were twins. Compared to singletons, the likelihood of stillbirths and early neonatal deaths among twins was 1.9 and 6.5 times greater, respectively. 2) The proportion of twins among early neonatal deaths tended to decrease, probably because of improvements in prenatal and perinatal care. 3) The critical period for early neonatal deaths in singletons and twins lasted for the three first days after birth. 4) The incidence of perinatal deaths among...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100003

Low doses of gamma ionizing radiation increase hprt mutant frequencies of TK6 cells without triggering the mutator phenotype pathway
Provedor de dados: Genetics and Molecular Biology
Autores: Ayres,Flávio Monteiro; Cruz,Aparecido Divino da; Steele,Patricia; Glickman,Barry W.
The TK6 lymphoblastoid cell line is known to be mismatch repair (MMR) and p53 proficient. Deficiency in MMR results in a mutator phenotype characterized by microsatellite instability (MSI) and increased hprt mutant frequency (MF). Increased hprt MF is also a biomarker of effect for exposure to ionizing radiation. In order to test if a mutator phenotype could be induced by low doses of gamma ionizing radiation, an hprt cloning assay and a MSI investigation were performed after radiation exposure. The spontaneous MF was 1.6 x 10-6. The groups exposed to 0.2, 0.5 and 1.0 Gy had hprt MFs of 2.3, 3.3 and 2.2 x 10-6, respectively. The spontaneous MSI frequency per allele in non-selected cells was 5.4 x 10-3, as evidenced at the loci D11S35, nm23-H1, D8S135 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutator phenotype; Hprt; Microsatellite instability; TK6 cells; Ionizing radiation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300027

Effect of self-fertilization on Biomphalaria tenagophila (Orbigny, 1835)  (Pulmonata: Planorbidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Tuan,Roseli; Simões,Luiz Carlos Gomes.
Biomphalaria tenagophila is a simultaneous hermaphrodite freshwater snail, which can breed by self and cross-fertilization. It is, therefore, well suited for studying reproduction as an evolutive strategy. Several characteristics (shell size and age at sexual maturity, egg fecundity and fertility) were analyzed in four consecutive self-fertilized generations and compared to cross-fertilized individuals. The reproductive parameters were similar in the two groups. Shell size was significantly greater in the fourth self-fertilized generation than in the cross-fertilized individuals.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400012

Chemoprotective effect of cysteamine against the induction of micronuclei by methyl methanesulfonate and cyclophosphamide
Provedor de dados: Genetics and Molecular Biology
Autores: Santos-Mello,Renato; Deimling,Luiz Irineu; Lauer Júnior,Claudio; Carvalho,Thaís Rieger de.
Cysteamine or 2-mercaptoethylamine (MEA) is an aminothiol with a well-known radioprotective action. No specific information is available in the literature about the possible chemoprotective action of MEA against genotoxic chemical agents. This paper presents the results of studies on the ability of MEA to protect mouse bone marrow polychromatic erythrocytes against the induction of micronuclei by alkylating agents such as methyl methanesulfonate (MMS) and cyclophosphamide (CP). We observed that MEA administered intraperitoneally 30 min before or 30 min after the administration of MMS or CP significantly reduced the frequency of micronucleated polychromatic erythrocytes (MNPCEs) induced by the alkylating agents. When MEA was administered in combination with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cysteamine; MEA; Micronuclei; Amifostine; WR-2721.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100027

Deferoxamine synergizes with transforming growth factor-β signaling in chondrogenesis
Provedor de dados: Genetics and Molecular Biology
Autores: Huang,Zheng; He,Guangxu; Huang,Yanke.
Abstract Osteoarthritis, also known as degenerative arthritis or degenerative joint disease, is an epidemic disease that affects millions of people worldwide. Despite extensive recent work on the cellular biology of osteoarthritis, the precise mechanisms involved are still poorly understood and there is no effective treatment for this disease. The role of transforming growth factor-beta (TGF-β) in promoting chondrogenesis and inducing the expression of cartilage-specific extracellular matrix molecules to form cartilage is well-established. Historically, TGF-β has been considered to prevent osteoarthritis, but recent work suggests that TGF-β overexpression accelerates the progression of osteoarthritis in vivo. Clinically, it is therefore important to limit...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chondrogenesis; Deferoxamine; Osteoarthritis; TGF-β.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400698

Optimizing expression and purification of an ATP-binding gene gsiA from Escherichia coli k-12 by using GFP fusion
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,Zhongshan; Xiang,Quanju; Wang,Guangjun; Wang,Haiyan; Zhang,Yizheng.
The cloning, expression and purification of the glutathione (sulfur) import system ATP-binding protein (gsiA) was carried out. The coding sequence of Escherichia coli gsiA, which encodes the ATP-binding protein of a glutathione importer, was amplified by PCR, and then inserted into a prokaryotic expression vector pWaldo-GFPe harboring green fluorescent protein (GFP) reporter gene. The resulting recombinant plasmid pWaldo-GFP-GsiA was transformed into various E. coli strains, and expression conditions were optimized. The effect of five E. coli expression strains on the production of the recombinant gsiA protein was evaluated. E. coli BL21 (DE3) was found to be the most productive strain for GsiA-GFP fusion-protein expression, most of which was insoluble...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Glutathione transporter; GsiA; Gene expression; Green fluorescent protein.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400019

Association of GNB3 C825T polymorphism with plasma electrolyte balance and susceptibility to hypertension
Provedor de dados: Genetics and Molecular Biology
Autores: Nejatizadeh,Azim; Kumar,Rahul; Stobdanand,Tsering; Pasha,Mohammad Qadar.
The role of G-protein activation in cardiovascular disorders is well-known. G-protein β3 subunit (GNB3) C825T polymorphism is associated with increased intracellular signal transduction. We investigated the role of the variant in plasma sodium and potassium concentrations and association with hypertension. 345 healthy controls and 455 patients with essential hypertension were enrolled. Plasma renin activity and aldosterone concentration were measured. The variant, typed by SNaPshot, was analyzed on an ABI Prism 3100 Genetic Analyzer and GeneScan. The TT genotype and T allele were over-represented in the patients (p &lt; 0.001, p &lt; 0.0001). Multiple-logistic regression disclosed that the risk of hypertension was significantly greater for TT (p &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GNB3 C825T polymorphism; Hypertension; G protein; Plasma electrolytes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400004

The association of very low-density lipoprotein receptor (VLDLR) haplotypes with egg production indicates VLDLR is a candidate gene for modulating egg production
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,ZhePeng; Meng,GuoHua; Li,Na; Yu,MingFen; Liang,XiaoWei; Min,YuNa; Liu,FuZhu; Gao,YuPeng.
Abstract The very low-density lipoprotein receptor (VLDLR) transports egg yolk precursors into oocytes. However, our knowledge of the distribution patterns of VLDLR variants among breeds and their relationship to egg production is still incomplete. In this study, eight single nucleotide polymorphisms (SNPs) that account for 87% of all VLDLR variants were genotyped in Nick Chick (NC, n=91), Lohmann Brown (LohB, n=50) and Lueyang (LY, n=381) chickens, the latter being an Chinese indigenous breed. Egg production by NC and LY chickens was recorded from 17 to 50 weeks. Only four similar haplotypes were found in NC and LohB, of which two accounted for 100% of all NC haplotypes and 92.5% of LohB haplotypes. In contrast, there was considerable haplotypic diversity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Egg production; Haplotype; Very low-density lipoprotein receptor (VLDLR).
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300380

Population prevalence of hereditary breast cancer phenotypes and implementation of a genetic cancer risk assessment program in southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Palmero,Edenir I.; Caleffi,Maira; Schüler-Faccini,Lavínia; Roth,Fernanda L.; Kalakun,Luciane; Netto,Cristina Brinkmann Oliveira; Skonieski,Giovana; Giacomazzi,Juliana; Weber,Bernadete; Giugliani,Roberto; Camey,Suzi A.; Ashton-Prolla,Patricia.
In 2004, a population-based cohort (the Núcleo Mama Porto Alegre - NMPOA Cohort) was started in Porto Alegre, southern Brazil and within that cohort, a hereditary breast cancer study was initiated, aiming to determine the prevalence of hereditary breast cancer phenotypes and evaluate acceptance of a genetic cancer risk assessment (GCRA) program. Women from that cohort who reported a positive family history of cancer were referred to GCRA. Of the 9218 women enrolled, 1286 (13.9%) reported a family history of cancer. Of the 902 women who attended GCRA, 55 (8%) had an estimated lifetime risk of breast cancer ³ 20% and 214 (23.7%) had pedigrees suggestive of a breast cancer predisposition syndrome; an unexpectedly high number of these fulfilled criteria for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Genetic counseling; Hereditary cancer syndromes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300004

Low levels of genetic diversity depicted from mitochondrial DNA sequences in a heavily exploited marine fish (Cynoscion acoupa, Sciaenidae) from the Northern coast of Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Rosa; Schneider,Horacio; Santos,Simôni; Vallinoto,Marcelo; Sain-Paul,Ulrich; Sampaio,Iracilda.
The acoupa weakfish (Cynoscion acoupa - Sciaenidae) is a marine species of croaker with estuarine-dependent behavior, found in the western Atlantic from Panama to Argentina. It is one of the most exploited food fish on the northern coast of Brazil. In this study, DNA sequences were determined from the entire control region (D-loop) of the mitochondrial genome of 297 individuals collected during seven different months between December 2003 and August 2005 on the northern coast of Brazil (Amapá and Pará). Genetic variability expressed by haplotype (h = 0,892) and nucleotide (pi = 0,003) diversities were low compared to other heavily exploited marine fish species from the western Atlantic and eastern Asia. AMOVA depicted a lack of genetic structuring among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cynoscion acoupa; Acoupa weakfish; Sciaenidae; Low genetic diversity; Mitochondrial DNA; D-loop.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300015

Genetic diversity and population structure of naturally rare Calibrachoa species with small distribution in southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: John,Ana Laura de Wallau; Mäder,Geraldo; Fregonezi,Jeferson N.; Freitas,Loreta B.
Abstract Calibrachoa is a South-American genus comprising 27 species, several considered endemic or rare; few were subjects in genetic studies. We attempted to generate new data about the phylogenetically related and rare species C. eglandulata, C. sendtneriana, C. serrulata, and C. spathulata concerning their genetic diversity and population structure, which, coupled with their known restricted distribution, could help access their conservation status and contribute to the study of the Brazilian biodiversity. We sequenced 88 individuals for plastid intergenic spacers and genotyped 186 individuals for five microsatellite loci. Compared among each other, C. sendtneriana and C. serrulata presented the highest values of genetic diversity [π% (sd) = 0.23...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plastid DNA; Microsatellites; Threatened species; Grasslands; Herbaceous species.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100108

Genetic divergence of rubber tree estimated by multivariate techniques and microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Gouvêa,Lígia Regina Lima; Rubiano,Luciana Benchimol; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Gonçalves,Paulo de Souza.
Genetic diversity of 60 Hevea genotypes, consisting of Asiatic, Amazonian, African and IAC clones, and pertaining to the genetic breeding program of the Agronomic Institute (IAC), Brazil, was estimated. Analyses were based on phenotypic multivariate parameters and microsatellites. Five agronomic descriptors were employed in multivariate procedures, such as Standard Euclidian Distance, Tocher clustering and principal component analysis. Genetic variability among the genotypes was estimated with 68 selected polymorphic SSRs, by way of Modified Rogers Genetic Distance and UPGMA clustering. Structure software in a Bayesian approach was used in discriminating among groups. Genetic diversity was estimated through Nei's statistics. The genotypes were clustered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genealogy; Genetic diversity; Hevea brasiliensis; Multivariate analysis; SSRs.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200018

Twinning rate trend in a population sample from the city of São Paulo, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Colletto,Gloria M.D.D.
A retrospective study was carried out on a sample consisting of 89,491 deliveries occurred from 1979 through 1998, in the city of São Paulo, SP, Brazil. These deliveries included 935 twin and 26 triplet births. The mean incidence per 1,000 deliveries was estimated at 10.45 for twins (5.34 for dizygotic and 5.11 for monozygotic twins) and 0.29 for triplets. The average maternal age increased significantly during this period for both twins and singletons. The temporal variation in years significantly influenced the increase of monozygotic twins and of triplets. Temporal variation and maternal age significantly influenced the dizygotic twinning rate increase. An increased proportion of monozygotic twins born to mothers aged 31 - 35 years was also detected.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dizygotic twinning rate; Monozygotic twinning rate; Maternal age; Gestational order; Gestational age.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300005

Genetic diversity in section Rhizomatosae of the genus Arachis (Fabaceae) based on microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Angelici,Carla M.L.C.D.; Hoshino,Andrea Akemi; Nóbile,Paula Macedo; Palmieri,Dario Abel; Valls,José F. Montenegro; Gimenes,Marcos A.; Lopes,Catalina Romero.
The genus Arachis (Fabaceae) native to South America, contains 80 species divided into nine sections, three of which contain species of special economic importance such as the cultivated peanut (Arachis hypogaea), belonging to the section Arachis, and some perennial forage species from sections Caulorrhizae and Rhizomatosae. We used microsatellite markers to assay genetic variability among 77 accessions of four species from section Rhizomatosae, the diploid Arachis burkartii (2n = 2x = 20) and the tetraploid Arachis glabrata, Arachis pseudovillosa and Arachis nitida (2n = 4x = 40). A total of 249 alleles were found in the fifteen loci analyzed and a high degree of intra and interspecific polymorphism was detected. The lowest intraspecific variation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachis; Genetic diversity; Microsatellite; Peanut; Rhizomatosae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100016

A canonical correlation analysis of the association between carcass and ham traits in pigs used to produce dry-cured ham
Provedor de dados: Genetics and Molecular Biology
Autores: Ventura,Henrique T.; Lopes,Paulo S.; Peloso,José V.; Guimarães,Simone E.F.; Carneiro,Antonio Policarpo S.; Carneiro,Paulo L.S.
The association between carcass and ham traits in a pig population used to produce dry-cured ham was studied using canonical correlation analysis. The carcass traits examined were hot carcass weight (HCW), backfat thickness (BT) and loin depth (LD), and the ham traits studied were gross ham weight (GHW), trimmed ham weight (THW), ham inner layer fat thickness (HIFT), ham outer layer fat thickness (HOFT), pH (pH) and the Göfo value. Carcass and ham traits are not independent. The canonical correlations (r) between the carcass and ham traits at 130 kg were 0.77, 0.24 and 0.20 for the first, second and third canonical pair, respectively, and were all significant (p &lt; 0.01) by the Wilks test. The corresponding canonical correlations between the three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canonical correlation; Carcass; Ham; Pig.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300014

In silico evaluation of the Eucalyptus transcriptome
Provedor de dados: Genetics and Molecular Biology
Autores: Vicentini,Renato; Sassaki,Flávio T.; Gimenes,Marcos A.; Maia,Ivan G.; Menossi,Marcelo.
The expressed sequence tags (ESTs) produced in the Forests project provide an invaluable opportunity to assess the Eucalyptus transcriptome. Besides providing information on the different proteins produced by this plant, it is possible to infer gene expression profiles because non-normalized cDNA libraries were used. The EST frequency from any gene is correlated to the transcript levels in the tissues from which the cDNA libraries were constructed. The goal of this work was to identify Eucalyptus genes that showed either differential expression pattern or were ubiquitously expressed in the tissues sampled in the Forests project. Six robust statistical tests and very restrictive rules were applied to gain confidence in the in silico data aiming to avoid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transcriptome; Eucalyptus; Tissue-specific; Statistics; Differential expression.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400002

Co-evolution model of Colletotrichum lindemuthianum (melanconiaceae, melanconiales) races that occur in some Brazilian regions
Provedor de dados: Genetics and Molecular Biology
Autores: Alzate-Marin,Ana Lilia; Barros,Everaldo Gonçalves de; Moreira,Maurílio Alves.
Colletotrichum lindemuthianum, the causal agent of anthracnose in the common bean (Phaseolus vulgaris L.), displays a high level of virulence diversity, which explains the large number of existing pathotypes. Several lines of evidence indicate that such diversity is, at least in part, due to plant and pathogen co-evolution. A co-evolution model based on the binary classification of 25 races identified in Brazil by inoculation of differential cultivars and random amplified polymorphic DNA (RAPD) data is proposed. In this model, races 8 and 64 that infected bean cultivar Cornell 49-242 (Are gene) and Mexico 222 (Mexico I gene) are considered to be sources of two important evolutionary routes. Inferences about undescribed races from Brazil could be made.
Tipo: Info:eu-repo/semantics/other
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100022

Methylation in the promoter regions of WT1, NKX6-1 and DBC1 genes in cervical cancer tissues of Uygur women in Xinjiang
Provedor de dados: Genetics and Molecular Biology
Autores: Wu,Dan; Zhang,Jinli; Fan,Peiwen; Li,Hongtao; Li,Dongmei; Pan,Huan; He,Hongchang; Ren,Xianxian; Pan,Zhenzhen; Shao,Renfu; Pan,Zemin.
Abstract This study aimed to explore: 1) DNA methylation in the promoter regions of Wilms tumor gene 1 (WT1), NK6 transcription factor related locus 1 gene (NKX6-1) and Deleted in bladder cancer 1 (DBC1) gene in cervical cancer tissues of Uygur women in Xinjiang, and 2) the correlation of gene methylation with the infection of HPV16/18 viruses. We detected HPV16/18 infection in 43 normal cervical tissues, 30 cervical intraepithelial neoplasia lesions (CIN) and 48 cervical cancer tissues with polymerase chain reaction (PCR) method. Methylation in the promoter regions of the WT1, NKX6-1 and DBC1 genes in the above-mentioned tissues was measured by methylation-specific PCR (MSP) and cloning sequencing. The expression level of these three genes was measured by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene methylation; Gene expression; HPV16/18; Cervical cancer; Uygur women.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100009

Antimutagenic activity of cashew apple (Anacardium occidentale Sapindales, Anacardiaceae) fresh juice and processed juice (cajuína) against methyl methanesulfonate, 4-nitroquinoline N-oxide and benzo[a]pyrene
Provedor de dados: Genetics and Molecular Biology
Autores: Melo-Cavalcante,Ana Amelia; Picada,Jaqueline N.; Rubensam,Gabriel; Henriques,João A.P.
Cashew apple juice (CAJ), produced from the native Brazilian cashew tree (Anacardium occidentale), and has been reported to have antibacterial, antifungal, antitumor, antioxidant and antimutagenic properties. Both the fresh unprocessed juice and the processed juice (cajuína in Portuguese) has been shown to consist of a complex mixture containing high concentrations of anacardic and ascorbic acids plus several carotenoids, phenolic compounds and metals. We assessed both types of juice for their antimutagenic properties against the direct mutagens methyl methanesulfonate (MMS) and 4-nitroquinoline-N-oxide (4-NQO) and the indirect mutagen benzo[a]pyrene (BaP) using pre-treatment, co-treatment and post-treatment assays with Salmonella typhimurium strains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimutagenicity; BaP; Cajuína; MMS; 4-NQO; Salmonella microsome assay.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400024

Cytogenetic analysis of three sea catfish species (Teleostei, Siluriformes, Ariidae) with the first report of Ag-NOR in this fish family
Provedor de dados: Genetics and Molecular Biology
Autores: Nirchio,Mauro; Martinez,Emanuel Ricardo Monteiro; Foresti,Fausto; Oliveira,Claudio.
Despite their ecological and economical importance, fishes of the family Ariidae are still genetically and cytogenetically poorly studied. Among the 133 known species of ariids, only eight have been karyotyped. Cytogenetic analyses performed on Genidens barbus and Sciades herzbergii revealed that both species have 2n = 56 chromosomes and Cathorops aff. mapale has 2n = 52 chromosomes: Genidens barbus has 10 Metacentrics (M), 14 Submetacentrics (SM), 26 Subtelocentrics (ST), and 6 Acrocentrics (A), Sciades herzbergii has 14M, 20SM, 18ST and 4A, whereas Cathorops aff. mapale has 14M, 20SM, and 18ST. The nucleolus organizer regions (NORs) were found in a single chromosome pair on the short arm of a large-sized ST pair in Genidens barbus and on the short arm of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Ag-NORs; Chromosome evolution; Chromosome rearrangements; Fish.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200011

Genotype-environment interaction and the number of test sites for the genetic improvement of rubber trees (Hevea) in São Paulo State, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Reginaldo Brito da; Resende,Marcos Deon Vilela de; Araujo,Antonio José de; Gonçalves,Paulo de Souza; Martins,Antonio Lúcio Mello.
The present study quantifies the possible genotype-environment interactions and determines the ideal number of test sites for rubber trees [Hevea brasiliensis (Willd ex Adr. de Juss.) Muell Arg] in the plateau region of São Paulo State. The study was based on the genetic correlation among progenies at three different sites and on estimates of genetic gains with indirect selection of rubber trees. Twenty-two half-sib progenies were planted at the Jaú, Pindorama and Votuporanga experimental stations using random blocks with five replications and 10 plants per plot. At three years of age, the plants were evaluated for their total number of latex ring vessels (NR), rubber production (RP), bark thickness (BT) and girth (SG). There was significant genetic...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100033

Quantitative inheritance of some wheat plant traits
Provedor de dados: Genetics and Molecular Biology
Autores: Novoselovic,D.; Baric,Marijana; Drezner,G.; Gunjaca,J.; Lalic,A.
The objective of this study was to estimate gene effects and genetic variability for some quantitative traits of two winter wheat crosses (Soissons/Zitarka and Soissons/Sana) by generation mean analysis. In most cases a digenic epistatic model was sufficient to explain variation in generation means. The additive-dominance model was adequate for plant height and grain weight per spike of the longest culm. In two cases (grain yield per plant and single grain weight) these models failed to explain variation in generation means, implying the presence of higher order interactions or interactions between linked loci. Dominance effects and additive x additive epistasis were more important than additive effects and other epistatic components. Only complementary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; Quantitative traits; Joint scaling test; Gene effects; Heritability.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100015

No contribution of GSTM1 and GSTT1 null genotypes to the risk of neutropenia due to benzene exposure in Southeastern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Carmen Silvia Passos; Lourenço,Gustavo Jacob; Lorand-Metze,Irene; Nascimento,Helvia; Saad,Sara Teresinha Ollala; Costa,Fernando Ferreira.
Exposure to benzene has been associated with haematological diseases such as neutropenia (NEB) and acute myeloid leukaemia (AML). We tested whether the null genotypes of the GSTM1 and GSTT1 genes, involved in benzene inactivation, altered the risk for NEB in southeastern Brazil. Genomic DNA from 55 NEB patients and 330 controls was analysed by multiplex-polymerase chain reaction. The frequency of the GSTM1, GSTT1 and combined null genotypes was similar in patients and controls (GSTM1, 27.3% vs. 38.8%, p = 0.16; GSTT1, 25.5% vs. 19.7%, p = 0.24; GSTM1/GSTT1, 12.7% vs. 6.7%, p = 0.26; respectively). The distribution of genotype classes in NEB patients was similar to normal controls, suggesting that GSTM1 and GSTT1 null genotypes make no specific contribution...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutropenia; Glutathione S-transferase; GSTM1; GSTT1.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400006

Occurrence and abundance of a mariner-like element in freshwater and terrestrial planarians (Platyhelminthes, Tricladida) from southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Sperb,Fernanda; Schuck,Desirée Cigaran; Rodrigues,Jaqueline Josi Samá.
Transposable elements are DNA sequences present in all the large phylogenetic groups, both capable of changing position within the genome and constituting a significant part of eukaryotic genomes. The mariner family of transposons is one of the few which occurs in a wide variety of taxonomic groups, including freshwater planarians. Nevertheless, so far only five planarian species have been reported to carry mariner-like elements (MLEs), although several different species have been investigated. Regarding the number of copies of MLEs, Girardia tigrina is the only planarian species in which this has been evaluated, with an estimation of 8,000 copies of the element per haploid genome. Preliminary results obtained in our laboratory demonstrated that MLE is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mariner; Planarian; Transposable element; Transposons.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400012

The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest
Provedor de dados: Genetics and Molecular Biology
Autores: Quintero,Nathalia; Stashenko,Elena E.; Fuentes,Jorge Luis.
In this work, the toxicity and genotoxicity of organic solvents (acetone, carbon tetrachloride, dichloromethane, dimethylsulfoxide, ethanol, ether and methanol) were studied using the SOS chromotest. The influence of these solvents on the direct genotoxicity induced by the mutagens mitomycin C (MMC) and 4-nitroquinoline-1-oxide (4-NQO) were also investigated. None of the solvents were genotoxic in Escherichia coli PQ37. However, based on the inhibition of protein synthesis assessed by constitutive alkaline phosphatase activity, some solvents (carbon tetrachloride, dimethylsulfoxide, ethanol and ether) were toxic and incompatible with the SOS chromotest. Solvents that were neither toxic nor genotoxic to E. coli (acetone, dichloromethane and methanol)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigenotoxicity; Genotoxicity; Interference; Mitomycin C; 4-nitro-quinoline-1-oxide; SOS chromotest; Solvents; Vitamin E.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300018

Effect of stannous chloride combined with caffeine on fecundity of Drosophila prosaltans
Provedor de dados: Genetics and Molecular Biology
Autores: Itoyama,Mary Massumi; Bicudo,Hermione Elly Melara de Campos.
The present study analyzed the number of progeny of stannous chloride- and/or caffeine-treated Drosophila prosaltans. A significant decreasing effect was observed in every case when compared to the control, except for the smallest stannous chloride dose used. Combinations of both substances using two different stannous chloride doses did not differ significantly from each other but number of progeny decreased 15% with the higher concentration combination when compared with caffeine-treated flies. The present results and data in the literature indicate that the effects of continuous ingestion of stannous chloride should be studied.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100019

Phylogenetic study of Class Armophorea (Alveolata, Ciliophora) based on 18S-rDNA data
Provedor de dados: Genetics and Molecular Biology
Autores: Paiva,Thiago da Silva; Borges,Bárbara do Nascimento; Silva-Neto,Inácio Domingos da.
The 18S rDNA phylogeny of Class Armophorea, a group of anaerobic ciliates, is proposed based on an analysis of 44 sequences (out of 195) retrieved from the NCBI/GenBank database. Emphasis was placed on the use of two nucleotide alignment criteria that involved variation in the gap-opening and gap-extension parameters and the use of rRNA secondary structure to orientate multiple-alignment. A sensitivity analysis of 76 data sets was run to assess the effect of variations in indel parameters on tree topologies. Bayesian inference, maximum likelihood and maximum parsimony phylogenetic analyses were used to explore how different analytic frameworks influenced the resulting hypotheses. A sensitivity analysis revealed that the relationships among higher taxa of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caenomorpha; Metopus; Nyctotherus; Sensitivity analysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400017

Early-onset breast cancer patients in the South and Southeast of Brazil should be tested for the TP53 p.R337H mutation
Provedor de dados: Genetics and Molecular Biology
Autores: Andrade,Kelvin C.; Santiago,Karina M.; Fortes,Fernanda P.; Mambelli,Lisley I.; Nóbrega,Amanda F.; Achatz,Maria I.
Abstract Germline TP53 mutations are associated with Li-Fraumeni syndrome (LFS), a disease that predisposes carriers to a wide variety of early onset tumors. In southern and southeastern Brazil, a high frequency of a germline TP53 mutation, p.R337H, was diagnosed in 0,3% of the population due to a founder effect. Carriers are at risk for developing cancer but the penetrance is lower than in typical DNA binding domain mutations. To date, only a few families were detected and diagnosis of carriers remains a challenge. Therefore, the inclusion of additional criteria to detect p.R337H carriers is necessary for the Brazilian population. We assessed the A.C. Camargo Cancer Center Oncogenetics Department database in search of common characteristics associated...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Breast cancer; Li-Fraumeni syndrome; P.R337H; TP53.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200199

DNA sequencing analysis of several G6PD variants previously defined by PCR-restriction enzyme analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Vaca,Gerardo; Arámbula Meraz,Eliakym.
Results of a corroborative DNA sequencing analysis for five glucose-6-phosphate dehydrogenase (G6PD) mutations previously defined by PCR-restriction enzyme analysis are presented. The suitability for performing DNA sequencing analysis is discussed along with the importance of selecting the proper PCR-REA strategy in order to define the presence of a specific mutation .
Tipo: Info:eu-repo/semantics/article
Palavras-chave: G6PD; Mutations; PCR-restriction enzyme analysis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100005

Anticlastogenic potential and antioxidant effects of an aqueous extract of pulp from the pequi tree (Caryocar brasiliense Camb)
Provedor de dados: Genetics and Molecular Biology
Autores: Khouri,Juliana; Resck,Inês S.; Poças-Fonseca,Marcio; Sousa,Thiago M.M.; Pereira,Luciana O.; Oliveira,Adriano B.B.; Grisolia,Cesar K.
The effectiveness of an aqueous extract of Caryocar brasiliense (Caryocaraceae) Camb pulp, popularly known in Brazil as pequi, against clastogenicity induced by cyclophosphamide and bleomycin was evaluated using an in vivo mouse bone marrow cell micronuclei test, an in vitro Chinese hamster ovary cell (CHO-K1) chromosome aberration test and an in vitro antioxidant assay based on the oxidative damage to 2-deoxy-D-ribose (2-DR) induced by hydroxyl radicals (•OH) generated by the reaction between ascorbic acid and (Fe III)-EDTA. In mouse bone marrow cells the extract showed a protective effect against micronuclei induced by cyclophosphamide and bleomycin but did not interfere with polychromatic bone marrow erythrocyte proliferation, except when the mice had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caryocar brasiliense; Anticlastogenicity; Antioxidant; Micronucleus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300024

Genetic diversity of Morato's Digger Toad, Proceratophrys moratoi: spatial structure, gene flow, effective size and the need for differential management strategies of populations
Provedor de dados: Genetics and Molecular Biology
Autores: Arruda,Mauricio P.; Costa,William P.; Recco-Pimentel,Shirlei M.
Abstract The Morato's Digger Toad, Proceratophrys moratoi, is a critically endangered toad species with a marked population decline in southern Brazilian Cerrado. Despite this, new populations are being discovered, primarily in the northern part of the distribution range, which raises a number of questions with regard to the conservation status of the species. The present study analyzed the genetic diversity of the species based on microsatellite markers. Our findings permitted the identification of two distinct management units. We found profound genetic structuring between the southern populations, on the left margin of the Tietê River, and all other populations. A marked reduction was observed in the contemporary gene flow among the central populations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proceratophrys moratoi; Microsatellite; Population structure; Management unit; Conservation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300502

A never-ending story: the steadily growing family of the FA and FA-like genes
Provedor de dados: Genetics and Molecular Biology
Autores: Gueiderikh,Anna; Rosselli,Filippo; Neto,Januario B.C.
Abstract Among the chromosome fragility-associated human syndromes that present cancer predisposition, Fanconi anemia (FA) is unique due to its large genetic heterogeneity. To date, mutations in 21 genes have been associated with an FA or an FA-like clinical and cellular phenotype, whose hallmarks are bone marrow failure, predisposition to acute myeloid leukemia and a cellular and chromosomal hypersensitivity to DNA crosslinking agents exposure. The goal of this review is to trace the history of the identification of FA genes, a history that started in the eighties and is not yet over, as indicated by the cloning of a twenty-first FA gene in 2016.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA repair; Leukemia; Fanconi anemia; Chromosomal abnormalities.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300398

The chloroplast genome of the desiccation-tolerant moss Pseudocrossidium replicatum (Taylor) R.H. Zander
Provedor de dados: Genetics and Molecular Biology
Autores: Cevallos,Miguel A.; Guerrero,Gabriela; Ríos,Selma; Arroyo,Analilia; Villalobos,Miguel Angel; Porta,Helena.
Abstract Mosses in conjunction with hornworts and liverworts are collectively referred to as bryophytes. These seedless, nonvascular plants are the closest extant relatives of early terrestrial plants and their study is essential to understand the evolutionary first steps of land plants. Here we report the complete chloroplast (cp) genome sequence of Pseudocrossidium replicatum, a moss belonging to the Pottiaceae family that is common in the central highlands of Mexico, in South America, in southern USA, and in Kenia. The cp genome (plastome) of P. replicatum is 123,512 bp in size, comprising inverted repeats of 9,886 bp and single-copy regions of 85,146 bp (LSC) and 18,594 bp (SSC). The plastome encodes 82 different proteins, 31 different tRNAs, and 4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bryophytes; Pottiaceae; Chloroplast; Next-generation sequencing.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300488

Antiproliferative effects of Tubi-bee propolis in glioblastoma cell lines
Provedor de dados: Genetics and Molecular Biology
Autores: Borges,Kleiton Silva; Brassesco,María Sol; Scrideli,Carlos Alberto; Soares,Ademilson Espencer Egea; Tone,Luiz Gonzaga.
Propolis is a resin formed by a complex chemical composition of substances that bees collect from plants. Since ancient times, propolis has been used in folk medicine, due to its biological properties, that include antimicrobial, anti-inflammatory, antitumoral and immunomodulatory activities. Glioblastoma is the most common human brain tumor. Despite the improvements in GBM standard treatment, patients' prognosis is still very poor. The aim of this work was to evaluate in vitro the Tubi-bee propolis effects on human glioblastoma (U251 and U343) and fibroblast (MRC-5) cell lines. Proliferation, clonogenic capacity and apoptosis were analyzed after treatment with 1 mg/mL and 2 mg/mL propolis concentrations for different time periods. Additionally,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glioblastoma; Propolis; Temozolomide; U251; U343.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200024

Linking dopamine neurotransmission and neurogenesis: the evolutionary history of the NTAD (NCAM1-TTC12-ANKK1-DRD2) gene cluster
Provedor de dados: Genetics and Molecular Biology
Autores: Mota,Nina Roth; Araujo-Jnr,Eli Vieira; Paixão-Côrtes,Vanessa Rodrigues; Bortolini,Maria Cátira; Bau,Claiton Henrique Dotto.
Genetic studies have long suggested the important role of the DRD2 gene in psychiatric disorders and behavior. Further research has shown a conjoined effect of genes in the Chr11q22-23 region, which includes the NCAM1, TTC12, ANKK1 and DRD2 genes, or NTAD cluster. Despite a growing need to unravel the role of this cluster, few studies have taken into account interspecies and evolutionary approaches. This study shows that behaviorally relevant SNPs from the NTAD cluster, such as rs1800497 (Taq1A) and rs6277, are ancient polymorphisms that date back to the common ancestor between modern humans and Neanderthals/Denisovans. Conserved synteny and neighborhood indicate the NTAD cluster seems to have been established at least 400 million years ago, when the first...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NTAD cluster; Taq1A SNP; Shared synteny; Co-regulation; Psychiatric genetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600004

Exome sequencing identifies a novel mutation of the GDI1 gene in a Chinese non-syndromic X-linked intellectual disability family
Provedor de dados: Genetics and Molecular Biology
Autores: Duan,Yongheng; Lin,Sheng; Xie,Lichun; Zheng,Kaifeng; Chen,Shiguo; Song,Hui; Zeng,Xuchun; Gu,Xueying; Wang,Heyun; Zhang,Linghua; Shao,Hao; Hong,Wenxu; Zhang,Lijie; Duan,Shan.
Abstract X-linked intellectual disability (XLID) has been associated with various genes. Diagnosis of XLID, especially for non-syndromic ones (NS-XLID), is often hampered by the heterogeneity of this disease. Here we report the case of a Chinese family in which three males suffer from intellectual disability (ID). The three patients shared the same phenotype: no typical clinical manifestation other than IQ score ≤ 70. For a genetic diagnosis for this family we carried out whole exome sequencing on the proband, and validated 16 variants of interest in the genomic DNA of all the family members. A missense mutation (c.710G &gt; T), which mapped to exon 6 of the Rab GDP-Dissociation Inhibitor 1 (GDI1) gene, was found segregating with the ID phenotype, and this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intellectual disability; GDI1 gene; Guanosine diphosphate dissociation inhibitor; Whole exome sequencing.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400591

Human population genetic structure detected by pain-related mu opioid receptor gene polymorphisms
Provedor de dados: Genetics and Molecular Biology
Autores: López Soto,Eduardo Javier; Catanesi,Cecilia Inés.
Several single nucleotide polymorphisms (SNPs) in the Mu Opioid Receptor gene (OPRM1) have been identified and associated with a wide variety of clinical phenotypes related both to pain sensitivity and analgesic requirements. The A118G and other potentially functional OPRM1 SNPs show significant differences in their allele distributions among populations. However, they have not been properly addressed in a population genetic analysis. Population stratification could lead to erroneous conclusions when they are not taken into account in association studies. The aim of our study was to analyze OPRM1 SNP variability by comparing population samples of the International Hap Map database and to analyze a new population sample from the city of Corrientes,...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: OPRM1; SNPs; A118G; AMOVA; Population genetics.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200152

Darcy Fontoura de Almeida (1930-2014)
Provedor de dados: Genetics and Molecular Biology
Autores: Vasconcelos,Ana Tereza Ribeiro de; Costa,Sergio Olavo Pinto da; Goldenberg,Samuel; Ferreira,Luís Carlos de Souza.
Tipo: Info:eu-repo/semantics/article
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300002

Karyotypes of two cytotypes of Paspalum quadrifarium Lam. (Poaceae): an alternative technique for small chromosomes in plants
Provedor de dados: Genetics and Molecular Biology
Autores: Speranza,Pablo; Vaio,Magdalena; Mazzella,Cristina.
Paspalum quadrifarium Lam. is a bunchgrass native to Uruguay, Argentina, and southern Brazil. Diploid, triploid, tetraploid and hexaploid cytotypes have been reported for this species of the Quadrifaria group of Paspalum. In this group, a high degree of cytogenetic homology between the genomes of several diploid species has been reported, based on meiotic pairing in interspecific hybrids; multivalent associations would thus be expected in polyploid hybrids. Karyotype analysis could provide useful information about the genomic architecture of polyploid plants; however, the fully condensed mitotic chromosomes of Paspalum do not provide enough morphological features for such an analysis. In this paper, we used mitotic prometaphase chromosomes treated with 70%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Meiosis; Prometaphase; Grasses; Paspalum.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400013

Searching for ancient balanced polymorphisms shared between Neanderthals and Modern Humans
Provedor de dados: Genetics and Molecular Biology
Autores: Viscardi,Lucas Henriques; Paixão-Côrtes,Vanessa Rodrigues; Comas,David; Salzano,Francisco Mauro; Rovaris,Diego; Bau,Claiton Dotto; Amorim,Carlos Eduardo G.; Bortolini,Maria Cátira.
Abstract Hominin evolution is characterized by adaptive solutions often rooted in behavioral and cognitive changes. If balancing selection had an important and long-lasting impact on the evolution of these traits, it can be hypothesized that genes associated with them should carry an excess of shared polymorphisms (trans- SNPs) across recent Homo species. In this study, we investigate the role of balancing selection in human evolution using available exomes from modern (Homo sapiens) and archaic humans (H. neanderthalensis and Denisovan) for an excess of trans-SNP in two gene sets: one associated with the immune system (IMMS) and another one with behavioral system (BEHS). We identified a significant excess of trans-SNPs in IMMS (N=547), of which six of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human behavior; Evolution; Balancing selection; Immune genes; Behavioral genes.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100067

An association study of FOXO3 variant and longevity
Provedor de dados: Genetics and Molecular Biology
Autores: Silva-Sena,Geralda Gillian; Camporez,Daniela; Santos,Lígia Ramos dos; Silva,Aline Sesana da; Sagrillo Pimassoni,Lúcia Helena; Tieppo,Alessandra; Pimentel Batitucci,Maria do Carmo; Morelato,Renato Lírio; Paula,Flavia de.
Abstract Human longevity is a polygenic and multifactorial trait. Pathways related to lifespan are complex and involve molecular, cellular, and environmental processes. In this analytical observational study, we evaluated the relationship between environment factors, oxidative stress status, DNA integrity level, and the association of FOXO3 (rs2802292), SOD2 (rs4880), APOE (rs429358 and rs7412), and SIRT1 (rs2273773) polymorphisms with longevity in oldest-old individuals from southeastern Brazil. We found an association between the FOXO3 GG genotype and gender. While lifestyle, anthropometric, and biochemical characteristics showed significant results, DNA damage and oxidative stress were not related to lifespan. We found that long-lived individuals with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lifespan; SNPs; Environmental factors; Oxidative stress; Genomic damage.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300386

Asymptotic weight and maturing rate in mice selected for body conformation
Provedor de dados: Genetics and Molecular Biology
Autores: Di Masso,Ricardo J.; Silva,Patricia S.; Font,María Teresa.
Growth patterns of four lines of mice selected for body conformation were analyzed with the logistic function, in order to provide baseline information about the relationship between asymptotic weight and maturing rate of body weight. Two lines were divergently selected favoring the phenotypic correlation between body weight and tail length (agonistic selection: CBi+, high body weight and long tail; CBi-, low body weight and short tail), whereas the other two lines were generated by a disruptive selection performed against the correlation between the aforementioned traits (antagonistic selection: CBi/C, high body weight and short tail; CBi/L, low body weight and long tail). The logistic parameters A (asymptotic weight) and k (maturing rate) behaved in...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200016

Ovarian development in Meliponine bees (Hymenoptera: Apidae): the effect of queen presence and food on worker ovary development and egg production
Provedor de dados: Genetics and Molecular Biology
Autores: Cruz-Landim,Carminda da.
Morphological studies of Meliponine worker ovaries in five species indicated a possible stimulatory effect of the queen on ovary development and on the production of trophic eggs in some of them. There are also indications that the queen inhibits the production of fertile eggs by the workers. This inhibition may involve a delay in the development of fertile eggs by the worker, until she is out of direct contact with the queen, or a lack of ovary development in the queen's presence, as seen in Leurotrigona muelleri. The evolutionary tendency toward inability to produce fertile eggs has its extreme representation in the pupal worker ovary reabsorption found in Frieseomelitta silvestri. On the other hand, the finding, in some species, of dwarf queens with the...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100015

Heterochromatin diversity and its co-localization with 5S and 45S rDNA sites in chromosomes of four Maxillaria species (Orchidaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Cabral,Juliano S.; Felix,Leonardo P.; Guerra,Marcelo.
We investigated four orchids of the genus Maxillaria (M. discolor, M. acicularis, M. notylioglossa and M. desvauxiana) in regard to the position of heterochromatin blocks as revealed using chromomycin A3 (CMA) and 4'-6-diamidino-2-phenylindole (DAPI) fluorochrome staining and 5S and 45S rDNA sites using fluorescence in situ hybridization (FISH). The species showed differences in chromosome number and a diversified pattern of CMA+ and DAPI+ bands, including heteromorphism for CMA+ bands. The 5S and 45S rDNA sites also varied in number and most of them were co-localized with CMA+ bands. The relationship between 5S rDNA sites and CMA+ bands was more evident in M. notylioglossa, in which the brighter CMA+ bands were associated with large 5S rDNA sites....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heterochromatin; CMA and DAPI; 45S and 5S rDNA; Maxillaria.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400015

Genetics of resistance to powdery mildew (Microsphaera diffusa) in Brazilian soybean populations
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Elaine Cristine Piffer; Di Mauro,Antonio Orlando; Centurion,Maria Aparecida Pessôa da Cruz.
Crosses between resistant and susceptible soybean cultivars were performed and the F2 populations were obtained to study the inheritance of soybean resistance to powdery mildew and to estimate the number and action of genes related to resistance. The reaction to powdery mildew was studied in a greenhouse and pots carrying plants with symptoms were distributed among the pots carrying the genotypes to be tested as a source of inoculum. Individual plants were scored according to the method of Yorinori (1997), with modifications, and classified as resistant or susceptible. The results showed that adult soybeans plants can present resistance to powdery mildew, which is controlled by one major gene with a dominant effect.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Powdery mildew; Glycine max; Genetic control.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300015

PCI-24781 down-regulates EZH2 expression and then promotes glioma apoptosis by suppressing the PIK3K/Akt/mTOR pathway
Provedor de dados: Genetics and Molecular Biology
Autores: Zhang,Wei; Lv,Shengqing; Liu,Jun; Zang,Zhenle; Yin,Junyi; An,Ning; Yang,Hui; Song,Yechun.
PCI-24781 is a novel histone deacetylase inhibitor that inhibits tumor proliferation and promotes cell apoptosis. However, it is unclear whether PCI-24781 inhibits Enhancer of Zeste 2 (EZH2) expression in malignant gliomas. In this work, three glioma cell lines were incubated with various concentrations of PCI-24781 (0, 0.25, 0.5, 1, 2.5 and 5 M) and analyzed for cell proliferation by the MTS 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay and colony formation, and cell cycle and apoptosis were assessed by flow cytometry. The expression of EZH2 and apoptosis-related proteins was assessed by western blotting. Malignant glioma cells were also transfected with EZH2 siRNA to examine how PCI-24781 suppresses tumor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EZH2; Malignant gliomas; PCI-24781; PIK3K/Akt/mTOR signaling pathway.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500015

Polymorphisms on SSC15q21-q26 Containing QTL for reproduction in Swine and its association with litter size
Provedor de dados: Genetics and Molecular Biology
Autores: Du,Hongli; Chen,Jing; Cui,Jianxun; Wang,Xiaoning; Zhang,Xiquan.
Several quantitative trait loci (QTL) for important reproductive traits (ovulation rate) have been identified on the porcine chromosome 15 (SSC15). To assist in the selection of positional candidate swine genes for these QTL on SSC15, twenty-one genes had already been assigned to SSC15 in a previous study in our lab, by using the radiation hybrid panel IMpRH. Further polymorphism studies were carried out on these positional candidate genes with four breeds of pigs (Duroc, Erhualian, Dahuabai and Landrace) harboring significant differences in reproduction traits. A total of nineteen polymorphisms were found in 21 genes. Among these, seven in six genes were used for association studies, whereby NRP2 polymorphism was found to be significantly (p &lt; 0.05)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Porcine; Reproductive traits; NRP2; Candidate gene.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100010

The genetic structure and mating system of Acrocomia aculeata (Arecaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Abreu,Aluana Gonçalves; Priolli,Regina Helena Geribello; Azevedo-Filho,Joaquim Adelino; Nucci,Stella Maris; Zucchi,Maria Imaculada; Coelho,Ricardo Marques; Colombo,Carlos Augusto.
Acrocomia aculeata is a perennial, fruit-producing palm tree, native to tropical forests. Its fruits have spurred interest because of their significant potential for use in the cosmetic industry and as feedstock for biofuel. In the present study, the genetic structure and mating system in Acrocomia aculeata were analyzed, using eight nuclear microsatellite loci and samples from São Paulo and Minas Gerais states, Brazil. By means of Bayesian analysis, these populations were clustered into two or three groups. A high multilocus outcrossing rate suggests that outcrosses were predominant, although a certain degree of biparental inbreeding also occurred. Thus, although monoecious and self-compatible, there is every indication that A. aculeata bears a mixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; Mating system.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100016

The rearranged mitochondrial genome of Leptopilina boulardi (Hymenoptera: Figitidae), a parasitoid wasp of Drosophila
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Daniel S.; Gomes,Tiago M.F.F.; Loreto,Elgion L.S.
Abstract The partial mitochondrial genome sequence of Leptopilina boulardi (Hymenoptera: Figitidae) was characterized. Illumina sequencing was used yielding 35,999,679 reads, from which 102,482 were utilized in the assembly. The length of the sequenced region of this partial mitochondrial genome is 15,417 bp, consisting of 13 protein-coding, two rRNA, and 21tRNA genes (the trnaM failed to be sequenced) and a partial A+T-rich region. All protein-coding genes start with ATN codons. Eleven protein-coding genes presented TAA stop codons, whereas ND6 and COII that presented TA, and T nucleotides, respectively. The gene pattern revealed extensive rearrangements compared to the typical pattern generally observed in insects. These rearrangements involve two...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mitogenome; Cynipoidea; Leptopilina; Parasitic wasp.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400611

Cytogenetics of two hylid frogs from Brazilian Cerrado
Provedor de dados: Genetics and Molecular Biology
Autores: Targueta,Cíntia Pelegrineti; Guerra,Vinícius; Gambale,Priscilla Guedes; Bastos,Rogério Pereira; Silva,Daniela de Melo e; Telles,Mariana Pires de Campos.
Abstract Cytogenetic data can be useful for taxonomic and phylogenetic studies, as well as to provide information about chromosome evolution. Therefore, it may help design conservation priorities for some threatened species, such as anurans. Herein, we describe the karyotypes of Scinax constrictus and Ololygon centralis, native endemic species from the Brazilian Cerrado. Chromosome preparations for both species were stained with Giemsa for morphological analyses and then impregnated by the Ag-NOR method for localization of the nucleolar organizer region (NOR). Both species had 24 chromosomes, as confirmed by meiotic analyses, which showed 12 bivalents. Chromosome morphologies presented the same pattern for Scinax and Ololygon compared to species already...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anura; Karyotype; Ololygon; Scinax.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500814

Chromosomal aberrations in peripheral lymphocytes from male native miners working in the Peruvian Andes
Provedor de dados: Genetics and Molecular Biology
Autores: Santa Maria,Sergio R.; Arana,Margarita; Ramirez,Oswaldo.
We analyzed the frequency of chromosomal aberrations in peripheral lymphocytes from underground miners from the Casapalca (n = 8, mean age = 45 y, range = 36 y to 55 y) and Bellavista (n = 8, mean age = 28 y, range 23 y to 34 y) high-altitude mining camps in the Peruvian Andes. This population was occupationally exposed to heavy metals such as lead and zinc as well as diesel emission particles, organic solvents and mine dust. The control groups consisted of individuals from a high altitude farming community in the Peruvian village of Tinco (n = 8, mean age = 37 y, range = 25 y to 52 y) and the sea level city of Lima (n = 14, mean age = 26 y, range = 20 y to 35 y). All individuals were male native Peruvians. A significantly higher incidence (1.88%, p &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal aberrations; High altitude; Mutagenesis; Peruvian Andes; Underground miners.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600017

Mitochondrial DNA-like sequence in the nuclear genome of Saguinus (Callitrichinae, Primates): transfer estimation
Provedor de dados: Genetics and Molecular Biology
Autores: Vallinoto,Marcelo; Sena,Leonardo; Sampaio,Iracilda; Schneider,Horacio; Schneider,Maria Paula.
Mitochondrial DNA-like sequences have been found in the nuclei of a variety of organisms. These nuclear pseudogenes can be used to estimate relative evolutionary rates of mitochondrial genes, and can be used as outgroups in phylogenetic analyses. In this study, mitochondrial sequences with pseudogene-like characteristics, including deletions and/or insertions and stop codons, were found in tamarins (Saguinus spp., Callitrichinae, Primates). Phylogenetic analysis allowed estimation of the timing of the migration of these sequences to the nuclear genome, and also permitted inferences on the phylogeny of the genus. The choice of an inadequate outgroup (Aotus infulatus) prevented a good phylogenetic resolution of the subfamily Callitrichinae. The relatively...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100006

The role of natural selection in human evolution – insights from Latin America
Provedor de dados: Genetics and Molecular Biology
Autores: Salzano,Francisco M.
Abstract A brief introduction considering Darwin's work, the evolutionary synthesis, and the scientific biological field around the 1970s and subsequently, with the molecular revolution, was followed by selected examples of recent investigations dealing with the selection-drift controversy. The studies surveyed included the comparison between essential genes in humans and mice, selection in Africa and Europe, and the possible reasons why females in humans remain healthy and productive after menopause, in contrast with what happens in the great apes. At the end, selected examples of investigations performed in Latin America, related to the action of selection for muscle performance, acetylation of xenobiotics, high altitude and tropical forest adaptations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Natural selection; Human evolution; Population genetics; Human adaptation; History of genetics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300302

Mutational landscape of head and neck squamous cell carcinomas in a South Asian population
Provedor de dados: Genetics and Molecular Biology
Autores: Ghias,Kulsoom; Rehmani,Sadiq S; Razzak,Safina A; Madhani,Sarosh; Azim,M. Kamran; Ahmed,Rashida; Khan,Mumtaz J.
Abstract Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer type globally and contributes significantly to burden of disease in South Asia. In Pakistan, HNSCC is among the most commonly diagnosed cancer in males and females. The increasing regional burden of HNSCC along with a unique set of risk factors merited a deeper investigation of the disease at the genomic level. Whole exome sequencing of HNSCC samples and matched normal genomic DNA analysis (n=7) was performed. Significant somatic single nucleotide variants (SNVs) were identified and pathway analysis performed to determine frequently affected signaling pathways. We identified significant, novel recurrent mutations in ASNS (asparagine synthetase) that may affect substrate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Head and neck squamous cell carcinoma (HNSCC); Whole exome sequencing; Driver mutation; Novel mutation; Pakistani population.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400526

Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks
Provedor de dados: Genetics and Molecular Biology
Autores: Dantas,Alisson F.; Lopes,Renata M.; Fascineli,Maria L.; José,Solange C.B.R.; Pádua,Juliano G.; Gimenes,Marcos A.; Grisolia,Cesar K.
Abstract This study aimed to assess the feasibility of comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. (rice) and Phaseolus vulgaris (beans) L. from gene banks. Rice and beans were exposed to methyl methanesulfonate (MMS) as a reference DNA damaging agent. Seeds of two accessions of rice and beans were obtained from Embrapa Rice and Beans - Brazil. Seed groups were imbibed in three concentrations of MMS for three periods of time to carry out cytogenetic tests, and for one period for the comet test. At concentrations of 10 and 15 mg/L, MMS induced cytotoxic and/or mutagenic effects in the meristematic cells of roots from all the accessions of both species. In the comet test, MMS induced genotoxic effects...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa L.; Phaseolus vulgaris L.; Methyl methanesulfonate; Cytogenetic test; Comet test.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100145

Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed
Provedor de dados: Genetics and Molecular Biology
Autores: Degrandi,Tiago Marafiga; Pita,Sebastian; Panzera,Yanina; Oliveira,Edivaldo Herculano C. de; Marques,José Ribamar Felipe; Figueiró,Marivaldo Rodrigues; Marques,Larissa Coêlho; Vinadé,Lucia; Gunski,Ricardo José; Garnero,Analía Del Valle.
Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the "river buffaloes" with 2n = 50 and the "swamp buffaloes", 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (Ag-NOR) and performed fluorescent in situ hybridization (FISH) experiments using 18S rDNA as probe. Metaphases were obtained through blood lymphocyte culture of 21 individuals, including river, swamp and hybrid cytotypes. Ag-NOR staining revealed active NORs on six chromosome pairs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 18S rDNA; Bubalus bubalis; FISH.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300009

Genetical-demographic data from two amazonian populations composed of descendants of african slaves: Pacoval and Curiau
Provedor de dados: Genetics and Molecular Biology
Autores: Guerreiro,João Farias; Ribeiro-dos-Santos,Ândrea Kely Campos; Santos,Eduardo José Melo dos; Vallinoto,Antonio Carlos Rosário; Cayres-Vallinoto,Izaura Maria Vieira; Aguiar,Gilberto Ferreira de Souza; Santos,Sidney Emanuel Batista dos.
The Amazon region of Brazil includes communities founded by escaped slaves, some of which still remain relatively isolated. We studied two such Afro-Brazilian communities (Pacoval and Curiau), in the rural area of Alenquer, Pará, and in the metropolitan region of Macapá, Amapá, respectively. Among 12 blood loci, alleles considered as markers of African ancestry, such as HBB*S, HBB*C, TF*D1, HP*2M, ABO*B, RH*D-, and CA2*2 were found at frequencies that are expected for populations with a predominantly African origin. Estimates of interethnic admixture indicated that the degree of the African component in Curiau (74%) is higher than that of Pacoval (44%); an Amerindian contribution was not detected in Curiau. Estimated values of African ancestry fit well...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200004

Gene pool sharing and genetic bottleneck effects in subpopulations of Eschweilera ovata (Cambess.) Mart. ex Miers (Lecythidaceae) in the Atlantic Forest of southern Bahia, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Alesandro S.; Borges,Daniela B.; Vivas,Caio V.; Berg,Cassio Van Den; Rodrigues,Polliana S.; Tarazi,Roberto; Gaiotto,Fernanda Amato.
Abstract Forest loss and fragmentation are the main threats to the maintenance of the Atlantic Forest, an important global biodiversity hotspot. Because of the current critical level of deforestation, ecological corridors are needed to facilitate species dispersion and gene flow among fragments. This study was conducted to investigate the genetic variability and gene pool sharing of Eschweilera ovata in five forest remnants in southern Bahia, Brazil using nuclear simple sequence repeat (nSSR) and plastid simple sequence repeat (cpSSR) microsatellite markers. cpSSR marker analysis revealed the domains of four haplotypes, showing that 80% of the individuals had only four maternal origins, reflecting a founder effect and/or genetic bottleneck. The results of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional connectivity; Tree species; Chloroplast; Founder effect; Simple sequence repeat development.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400655

Molecular characterization of beta-tubulin from Phakopsora pachyrhizi, the causal agent of Asian soybean rust
Provedor de dados: Genetics and Molecular Biology
Autores: Maciel,Talles Eduardo Ferreira; Freire,Maíra Cristina Menezes; Almeida,Álvaro M.R. de; Oliveira,Luiz Orlando de.
β-tubulins are structural components of microtubules and the targets of benzimidazole fungicides used to control many diseases of agricultural importance. Intron polymorphisms in the intron-rich genes of these proteins have been used in phylogeographic investigations of phytopathogenic fungi. In this work, we sequenced 2764 nucleotides of the β-tubulin gene (Pp tubB) in samples of Phakopsora pachyrhizi collected from seven soybean fields in Brazil. Pp tubB contained an open reading frame of 1341 nucleotides, including nine exons and eight introns. Exon length varied from 14 to 880 nucleotides, whereas intron length varied from 76 to 102 nucleotides. The presence of only four polymorphic sites limited the usefulness of Pp tubB for phylogeographic studies in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asian soybean rust; Beta-tubulin; Genetic diversity; Phakopsora pachyrhizi; Soybeans.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200025

Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2)
Provedor de dados: Genetics and Molecular Biology
Autores: Rizo,Walace Fraga; Ferreira,Luis Eduardo; Colnaghi,Vanessa; Martins,Juliana Simões; Franchi,Leonardo Pereira; Takahashi,Catarina Satie; Beleboni,Rene Oliveira; Marins,Mozart; Pereira,Paulo Sérgio; Fachin,Ana Lúcia.
Cancer has become a major public health problem worldwide and the number of deaths due to this disease is increasing almost exponentially. In the constant search for new treatments, natural products of plant origin have provided a variety of new compounds to be explored as antitumor agents. Tabernaemontana catharinensis is a medicinal plant that produces alkaloids with expressive antitumor activity, such as heyneanine, coronaridine and voacangine. The aim of present study was firstly to screen the cytotoxic activity of the indole alkaloids heyneanine, coronaridine and voacangine against HeLa (human cervix tumor), 3T3 (normal mouse embryo fibroblasts), Hep-2 (human laryngeal epithelial carcinoma) and B-16 (murine skin) cell lines by MTT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 3T3; Apoptosis; Comet assay; Indole alkaloid; LDH.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100015

Molecular sexing of tucuxi dolphins (Sotalia guianensis and Sotalia fluviatilis) using samples from biopsy darting and decomposed carcasses
Provedor de dados: Genetics and Molecular Biology
Autores: Cunha,Haydée A.; Solé-Cava,Antonio M.
We tested the zinc-finger sex chromosome-linked genes Zfx/Zfy and the sex-determining region Y (Sry) genes for gender determination of biopsy samples from marine and riverine tucuxi dolphins (Sotalia guianensis and S. fluviatilis). We also evaluated the performance of these genes with decomposed carcasses, for which sexing cannot rely on the direct examination of the reproductive tract. Both systems proved reliable for sexing 46 fresh and decomposed samples, making them especially useful when biopsy darting is coupled with photo-identification studies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cetaceans; Remote sampling; Sex determination; Sry; Zfx/Zfy.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600025

Karyotype characterization and nucleolar organizer regions of marsupial species (Didelphidae) from areas of Cerrado and Atlantic Forest in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Núbia P.; Ventura,Karen; Silva Júnior,Magno C.; Silva,Daniela de Melo e; Yonenaga-Yassuda,Yatiyo; Pellegrino,Katia C. M.
The karyotypes of 23 specimens belonging to 16 species from nine genera of Brazilian marsupials (family Didelphidae) were studied. The animals were collected in eight localities of Cerrado or Atlantic Forest biomes in the states of Goiás, Tocantins and São Paulo. The karyotypes were analyzed after conventional Giemsa staining and silver staining of the nucleolus organizer regions (Ag-NORs). New karyotypic data were obtained for  Gracilinanus microtarsus  (2n = 14, FN = 24), Marmosops paulensis  (2n = 14, FN = 24) , Micoreus paraguayanus  (2n = 14, FN = 20) and  Monodelphis rubida  (2n = 18, FN = 32) and are discussed in detail. The karyotypes of  G. microtarsus ,  M. paulensis  and  M. paraguayanus  include three large pairs of submetacentrics (pairs 1, 2...
Palavras-chave: Marsupials; Karyotypes; Cytogenetics; Didelphidae; NORs.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500014

In silico analysis of alpha1-antitrypsin variants: the effects of a novel mutation
Provedor de dados: Genetics and Molecular Biology
Autores: Denden,Sabri; Leban,Nadia; Hayek,Donia; Knani,Jalel; Chibani,Jemni Ben; Khelil,Amel Haj.
Alpha1-antitrypsin (AAT) is a highly polymorphic protein with more than 120 variants that are classified as normal (normal protein secretion), deficient (reduced circulating AAT level caused by defective secretion) or null (no protein secretion). Alpha1-antitrypsin deficiency, one of the most common genetic disorders, predisposes adults to pulmonary emphysema and, to a lesser extent, chronic liver disease and cirrhosis. In this report, we provide additional sequence data for alpha1-antitrypsin based on the characterization of a novel variant detected in a 53-year-old heterozygous patient with chronic obstructive pulmonary disease. The mutation occurred on a PI*M2 base allele and was characterized by a T → C transition at nt 97 in exon II that led to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha1-antitrypsin; Computational analysis; Damaging mutation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400006

RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean
Provedor de dados: Genetics and Molecular Biology
Autores: Martins Filho,Sebastião; Sediyama,Carlos Sigueyuki; Moreira,Maurilio Alves; Barros,Everaldo Gonçalves de.
The soybean (Glycine max (L.) Merrill) frogeye leaf spot is caused by the fungus Cercospora sojina Hara and is a widespread disease in Brazil and other countries, causing severe losses in grain yield and also affecting seed quality. The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated. Bulked segregant analysis was applied to 3 F2 populations derived from crosses between the resistant cultivars Parana, Cristalina and Uberaba, and the susceptible cultivar Bossier. In the cross 'Parana' x 'Bossier', 2 RAPD markers were identified, CSOPA1(800C) and CSOPA2(1,250C), located at 4.4 ± 1.8 centiMorgans (cM) and 3.4 ± 1.7 cM respectively from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; RAPD; SCAR; Soybean; Cercospora sojina; Frogeye leaf spot.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300012

Molecular genetic diversity in populations of the stingless bee Plebeia remota: A case study
Provedor de dados: Genetics and Molecular Biology
Autores: Francisco,Flávio de Oliveira; Santiago,Leandro Rodrigues; Arias,Maria Cristina.
Genetic diversity is a major component of the biological diversity of an ecosystem. The survival of a population may be seriously threatened if its genetic diversity values are low. In this work, we measured the genetic diversity of the stingless bee Plebeia remota based on molecular data obtained by analyzing 15 microsatellite loci and sequencing two mitochondrial genes. Population structure and genetic diversity differed depending on the molecular marker analyzed: microsatellites showed low population structure and moderate to high genetic diversity, while mitochondrial DNA (mtDNA) showed high population structure and low diversity in three populations. Queen philopatry and male dispersal behavior are discussed as the main reasons for these findings.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; Meliponini; Microsatellites; Philopatry; Population genetics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100017

Karyotype characterization of Crotalaria juncea (L.) by chromosome banding and physical mapping of 18S-5.8S-26S and 5S rRNA gene sites
Provedor de dados: Genetics and Molecular Biology
Autores: Mondin,Mateus; Santos-Serejo,Janay A.; Aguiar-Perecin,Margarida L.R.
The chromosomes of Crotalaria juncea, a legume of agronomic interest with a 2n = 16 karyotype composed of metacentric chromosomes, were analyzed using several cytogenetic techniques. C-banding revealed heterochromatic regions around the centromeres in all chromosomes and adjacent to the secondary constriction on the chromosome 1 short arm. Fluorescent staining with the GC-specific chromomycin A3 (CMA) highlighted these heterochromatic regions and a tiny site on the chromosome 1 long arm while the AT-specific stain 4'-6-diamidino-2-phenylindole (DAPI) induced a reversed pattern. Staining with CMA combined with AT-specific distamycin A (DA) counterstaining quenched the pericentromeric regions of all chromosomes, but enhanced fluorescence was observed at the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalaria juncea karyotype; C-bands; Fluorescent banding; FISH; RDNA sites; Ag-NOR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100013

Characterization of 13 microsatellite loci developed from Meconopsis horridula
Provedor de dados: Genetics and Molecular Biology
Autores: Zhao,Yu; Zhang,Shi-Bao; Yang,Jing; Zhang,Lu.
Meconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic structure; Heterozygosity; Meconopsis horridula; Microsatellite markers; Polymorphism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300026

Cytogenetic variability in three species of the genus Cicindela (s.l.) (Coleoptera, Cicindelidae): karyotypes and localization of 18S rDNA genes
Provedor de dados: Genetics and Molecular Biology
Autores: Proença,Sónia J. R.; Collares-Pereira,Maria João; Serrano,Artur R. M.
Three tiger beetle species from the Cicindelini tribe were examined cytogenetically and found to have the following karyotypes: Cicindela argentata, 2n = 18 + X1X2Y/X1X1X2X 2; Cicindela aurulenta, 2n=18 + X1X2X3Y/X1X1X 2X2X3X3 and Cicindela suturalis, 2n = 18 + X1X2X3X4Y/X1X 1X2X2X3X3X4X 4. Fluorescence in situ hybridization (FISH) using a PCR-amplified 18S rDNA fragment as a probe showed the presence of ribosomal clusters in two autosomes in C. argentata, two autosomes and two heterosomes in C. aurulenta and in two heterosomes in C. suturalis (male configuration), revealing two new patterns of rDNA localization. Such results are representative of the cytogenetic variability observed in the species rich genus Cicindela (sensu lato) mainly as regards the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome evolution; Cicindela; Multiple sex chromosomes; RDNA localization; Tiger beetles.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400015

Trypanosomatid comparative genomics: contributions to the study of parasite biology and different parasitic diseases
Provedor de dados: Genetics and Molecular Biology
Autores: Teixeira,Santuza M.; Paiva,Rita Márcia Cardoso de; Kangussu-Marcolino,Monica M.; DaRocha,Wanderson D.
In 2005, draft sequences of the genomes of Trypanosoma brucei, Trypanosoma cruzi and Leishmania major, also known as the Tri-Tryp genomes, were published. These protozoan parasites are the causative agents of three distinct insect-borne diseases, namely sleeping sickness, Chagas disease and leishmaniasis, all with a worldwide distribution. Despite the large estimated evolutionary distance among them, a conserved core of ~6,200 trypanosomatid genes was found among the Tri-Tryp genomes. Extensive analysis of these genomic sequences has greatly increased our understanding of the biology of these parasites and their host-parasite interactions. In this article, we review the recent advances in the comparative genomics of these three species. This analysis also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma brucei; Trypanosoma cruzi; Leishmania major; Genome; RNAseq.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100001

GATM, the human ortholog of the mouse imprinted Gatm gene, escapes genomic imprinting in placenta
Provedor de dados: Genetics and Molecular Biology
Autores: Miyamoto,Toshinobu; Sengoku,Kazuo; Hayashi,Hiroaki; Sasaki,Yoshihito; Jinno,Yoshihiro; Ishikawa,Mutsuo.
The GATM gene encodes L-arginine:glycine amidinotransferase, which catalyzes the conversion of L-arginine into guanidinoacetate, the rate-limiting step in the synthesis of creatine. Since, deficiencies in creatine synthesis and transport lead to certain forms of mental retardation in human, the human GATM gene appears to be involved in brain development. Recently it has been demonstrated that the mouse Gatm is expressed during development and is imprinted with maternal expression in the placenta and yolk sac, but not in embryonic tissues. We investigated the imprinting status of the human GATM by analyzing its expression in four human placentas. GATM was biallelically expressed, thus suggesting that this gene escapes genomic imprinting in placentas,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GATM gene; Genomic imprinting; DNA polymorphism.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100008

Somaclonal variation for disease resistance in wheat and production of dihaploids through wheat x maize hybrids
Provedor de dados: Genetics and Molecular Biology
Autores: Mehta,Yeshwant R.; Angra,Dilma C.
Seven wheat cultivars having some degree of resistance to Bipolaris sorokiniana, Magnaporthe grisea or Xanthomonas campestris pv. undulosa (Xcu) provided somaclonal variation for disease resistance. Callus induction varied from 69.4 to 100% across the cultivars, whereas regeneration frequency of R1 plants (regenerated plants of first generation) varied between 2.7 and 23.1%. Resistance variation in the R2-regenerated second generation plants was observed for B. sorokiniana and M. grisea but not for Xcu. Attempts were made to fix the resistance characteristics of R3-regenerated third generation somaclones through wheat x maize hybrids. Wheat and maize hybridization of seven wheat somaclones yielded 81 embryos. A total of 11,624 somaclone florets were...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300021

A karyotypic study of three southern Brazilian Asteraceae species using fluorescence in situ hybridization with a 45S rDNA probe and C-CMA3 banding
Provedor de dados: Genetics and Molecular Biology
Autores: Fregonezi,Jéferson N.; Torezan,José M.D.; Vanzela,André L.L.
The Asteraceae, one of the largest families of flowering plants, contains about 1,100 genera and 20,000 species, and is well known for its extensive karyotypic variation. In this study, conventional Feulgen staining, C-CMA3 banding, and fluorescence in situ hybridization with a 45S rDNA probe were used to determine the chromosome number and the number and physical position of GC-rich heterochromatin and 45S rDNA sites in three Asteraceae weed species (Crepis japonica, Galinsoga parviflora and Chaptalia nutans). The three species exhibited karyotype differences in the chromosome number and shape, as a commom feature of Asteraceae. However, the 45S rDNA sites always occurred on the short chromosomal arms, associated with GC-heterochromatin. Althought of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CMA3; FISH; Heterochromatin; Polyploidy; 45S rDNA.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200016

A patient presenting a 22q13 deletion associated with an apparently balanced translocation t(16;22): an illustrative case in the investigation of patients with low ARSA activity
Provedor de dados: Genetics and Molecular Biology
Autores: Artigalás,Osvaldo; Paskulin,Giorgio; Riegel,Mariluce; Burin,Maira; Saraiva-Pereira,Maria Luiza; Maluf,Sharbel; Kiss,Andrea; Schwartz,Ida Vanessa D.
A 10-year-old speechless, mentally deficient male, with low arylsulfatase A (ARSA) activity, and presumably, methachromatic leukodystrophy, underwent genetic evaluation. As the clinical picture was not compatible with this diagnosisan ARSA gene and chromosome analysis were performed, showing the presence of a pseudodeficiency ARSA allele and a de novo apparently balanced t(16;22)(p11.2;q13) translocation. A deletion on the long arm of chromosome 22 encompassing the ARSA gene, as shown by FISH and array-CGH, indicated a 22q13 deletion syndrome. This case illustrates the importance of detailed cytogenetic investigation in patients presenting low arylsulfatase A activity and atypical/unspecific clinical features.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 22q13 deletion; Apparently balanced translocation; ARSA gene; Arylsulfatase A pseudodeficiency; Metachromatic leukodystrophy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300007

Information and Diagnosis Networks – tools to improve diagnosis and treatment for patients with rare genetic diseases
Provedor de dados: Genetics and Molecular Biology
Autores: Vieira,Taiane Alves; Trapp,Franciele Barbosa; Souza,Carolina Fischinger Moura de; Faccini,Lavínia Schuler; Jardim,Laura Bannach; Schwartz,Ida Vanessa Doederlein; Riegel,Mariluce; Vargas,Carmen Regla; Burin,Maira Graeff; Leistner-Segal,Sandra; Ashton-Prolla,Patrícia; Giugliani,Roberto.
Abstract Brazil is a country of continental dimensions and most genetic services are concentrated in the Southeast and South, including the Medical Genetics Service of the Hospital de Clínicas de Porto Alegre (MGS/HCPA). As many areas on the country do not have adequate medical genetics support, networks were designed to extend the service of the MGS/HCPA reference center. This paper presents the information and diagnosis networks that have their headquarters at MGS/HCPA: SIAT (National Information System on Teratogenic Agents), SIEM (Information Service on Inborn Errors of Metabolism), Alô Genética (Hello Genetics - Medical Genetics Information Service for Primary Health Care Professionals); Rede MPS Brasil (MPS-Mucopolysaccharidosis Brazil Network); Rede...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Information services; Medical Genetics; Diagnostic networks; Rare diseases; Reference centers.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200155

Cytogenetic characterization of two species of Frieseomelitta Ihering, 1912 (Hymenoptera, Apidae, Meliponini)
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Antônio F.; Costa,Marco Antônio.
The cytogenetic analysis of Frieseomelitta dispar and F. francoi revealed the chromosome numbers 2n = 30 and n = 15 and a karyotypic formula 2K = 4M+2Mt+4A+20A M. The number of chromosomes observed was consistent with those reported for other Frieseomelitta species. The occurrence of the Mt chromosome and other features of the karyotype formulae suggest a close relationship between F. dispar, F. francoi and F. varia. Nevertheless, it was possible to differentiate the karyotypes of the species by DAPI/CMA3 staining, which revealed GC-rich regions on two chromosome pairs of F. dispar: one acrocentric and one pseudoacrocentric. In F. francoi, the same kinds of regions were observed on a pair of metacentrics and on a pair of acrocentrics. Our analysis also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hymenoptera; Frieseomelitta; Interspecific differentiation; C-banding; Heterochromatin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200012

Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assay
Provedor de dados: Genetics and Molecular Biology
Autores: Boriollo,Marcelo Fabiano Gomes; Resende,Marielly Reis; Silva,Thaísla Andrielle da; Públio,Juliana Yoshida; Souza,Luiz Silva; Dias,Carlos Tadeu dos Santos; Oliveira,Nelma de Mello Silva; Fiorini,João Evangelista.
The aim of this study was to evaluate the mutagenicity (clastogenicity/aneugenicity) of a glycolic extract of Ziziphus joazeiro bark (GEZJ) by the micronucleus assay in mice bone marrow. Antimutagenic activity was also assessed using treatments associated with GEZJ and doxorubicin (DXR). Mice were evaluated 24-48 h after exposure to positive (N-nitroso-N-ethylurea, NEU - 50 mg.kg-1 and DXR - 5 mg.kg-1) and negative (150 mM NaCl) controls, as well as treatment with GEZJ (0.5-2 g.kg-1), GEZJ (2 g.kg-1) + NEU and GEZJ (2 g.kg-1) + DXR. There were no significant differences in the frequencies of micronucleated polychromatic erythrocytes in mice treated with GEJZ and GEJZ + DXR compared to the negative controls, indicating that GEZJ was not mutagenic. Analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimutagenicity; Bone marrow; Doxorubicin; Micronucleus assay; Mutagenicity; Zizyphus joazeiro Mart. (raspa-de-Juá).
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300016

The genetics and conservation of Araucaria angustifolia: I. Genetic structure and diversity of natural populations by means of non-adaptive variation in the state of Santa Catarina, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Auler,Neiva Maria Frizon; Reis,Maurício Sedrez dos; Guerra,Miguel Pedro; Nodari,Rubens Onofre.
The objective of this study was to generate information relative to the allele distribution and dynamics within and among populations of Araucaria angustifolia, a naturally-occurring conifer in the south of Brazil, being known popularly as "pinheiro-do-Paraná", "araucaria" or pine tree. In order to elucidate the levels and the distribution of the genetic variability, the population's genetic structure and the genetic distance among natural populations of this species with different levels of disturbance in different geographical areas were studied in detail. For this, samples of leaf tissue were collected from 328 adult individuals in nine natural populations in Santa Catarina State. To analyze the samples, the allozyme technique was applied in starch gel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pinheiro; Forest degradation; Genetic diversity; Genetic erosion.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300014

NS3 protease from flavivirus as a target for designing antiviral inhibitors against dengue virus
Provedor de dados: Genetics and Molecular Biology
Autores: Natarajan,Satheesh.
The development of novel therapeutic agents is essential for combating the increasing number of cases of dengue fever in endemic countries and among a large number of travelers from non-endemic countries. The dengue virus has three structural proteins and seven non-structural (NS) proteins. NS3 is a multifunctional protein with an N-terminal protease domain (NS3pro) that is responsible for proteolytic processing of the viral polyprotein, and a C-terminal region that contains an RNA triphosphatase, RNA helicase and RNA-stimulated NTPase domain that are essential for RNA replication. The serine protease domain of NS3 plays a central role in the replicative cycle of dengue virus. This review discusses the recent structural and biological studies on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiviral inhibitor; Drug discovery; Multifunctional protein; NS3; Protease.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200002

ANRIL rs1333049 C/G polymorphism and coronary artery disease in a North Indian population - Gender and age specific associations
Provedor de dados: Genetics and Molecular Biology
Autores: Kaur,Naindeep; Singh,Jagtar; Reddy,Sreenivas.
Abstract Many studies conducted worldwide substantiate a role of genetic polymorphisms in non-coding regions linked with coronary artery disease (CAD). One such single nucleotide polymorphism (SNP) of a non-coding RNA in the INK4 locus (ANRIL) i.e. rs1333049 C/G in the vicinity of cell cycle regulating genes is documented to have a role in CAD risk. In this study we aimed to determine the association of ANRIL rs1333049 C/G with CAD in a North Indian population. Five hundred disease free controls and 500 CAD patients were genotyped using allele specific ARMS-PCR method. High risk association of rs1333049 was seen in both heterozygous and mutant genotypes (OR=2.883, 95% CI=1.475-5.638 and p=0.002 and OR=6.717, 95% CI=3.444-13.102 and p &lt; 0.001...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic polymorphism; Coronary artery disease; North Indian Population; ARMS-PCR; Epidemiology study.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100114

X-linked adrenoleukodystrophy: clinical and laboratory findings in 15 Brazilian patients
Provedor de dados: Genetics and Molecular Biology
Autores: Vargas,Carmen R.; Coelho,Daniella de M.; Barschak,Alethéa G.; Souza,Carolina F.M. de; Puga,Ana C.S.; Schwartz,Ida V.D.; Jardim,Laura; Giugliani,Roberto.
Adrenoleukodystrophy (X-ALD) is an X-linked recessively inherited peroxisomal disorder, phenotypically heterogeneous, characterized by progressive white-matter demyelination of the central nervous system and adrenocortical insufficiency. We investigated 15 male X-ALD patients varying in age from 7 to 39, diagnosed among 108 suspected patients referred for investigation. Plasma levels of very long chain fatty acids (VLCFA) were measured at our laboratory using gas chromatography (GC). Eleven cases of childhood X-ALD and four cases of adrenomyeloneuropathy (AMN) were diagnosed. Adrenal leukodystrophy insufficiency and limb weakness were the most frequent symptoms, appearing in 12, 8 and 6 of the patients, respectively. Physician awareness of X-ALD seems...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200002

Performance of common bean families after different generations under natural selection
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Nara Oliveira; Ramalho,Magno Antonio Patto; Abreu,Ângela de Fátima Barbosa; Carneiro,José Eustáquio de Souza.
A segregant bulk population derived from a single cross between the Carioca MG cultivar and the ESAL 686 line was used to investigate whether the action of natural selection in the direction required by the breeders and the delaying line extraction would increase the chance of obtaining families with greater grain yield. The populations were advanced from F2 to F24 and obtained families F2, F8 and F24 from the plants. These families and their parents were assessed for grain yield (kg/ha) in Lavras-MG in three sowing seasons (July 2001, November 2001 and March 2002) in an 18 x 18 lattice design with two replications in the first sowing and three in the other two. The largest mean yield, regardless of sowing season, was among the families derived from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Common bean; Natural selection; Bulk breeding method.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400018

Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene
Provedor de dados: Genetics and Molecular Biology
Autores: Nerys-Junior,Arildo; Braga-Dias,Luciene P.; Pezzuto,Paula; Cotta-de-Almeida,Vinícius; Tanuri,Amilcar.
Abstract The human C-C chemokine receptor type-5 (CCR5) is the major transmembrane co-receptor that mediates HIV-1 entry into target CD4+ cells. Gene therapy to knock-out the CCR5 gene has shown encouraging results in providing a functional cure for HIV-1 infection. In gene therapy strategies, the initial region of the CCR5 gene is a hotspot for producing functional gene knock-out. Such target gene editing can be done using programmable endonucleases such as transcription activator-like effector nucleases (TALEN) or clustered regularly interspaced short palindromic repeats (CRISPR-Cas9). These two gene editing approaches are the most modern and effective tools for precise gene modification. However, little is known of potential differences in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CCR5; CRISPR-Cas9; Efficiency; Gene editing; TALEN.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100167

Temperature and the progeny sex-ratio in Sciara ocellaris (Diptera, Sciaridae)
Provedor de dados: Genetics and Molecular Biology
Autores: Nigro,Rogério G.; Campos,Maria Cristina C.; Perondini,André Luiz P.
We found that the sex-ratio of an amphigenic strain of Sciara ocellaris varied widely from progenies with few males to progenies containing a larger proportion of males, with single-sex progenies being rare. The sex-ratio distributions were dependent on the temperature at which the stocks of flies were raised, with the sex-ratio distributions being symmetrical (i.e. about 50% males) at 18 °C and 20 °C while at the higher temperatures of 24 °C and 28 °C the distributions were skewed toward a high proportion of females with the mean proportion of males decreasing to about 30-37% per progeny. Temperature-shift experiments showed that high temperatures were effective only during the last stages of female pupal development plus a period after adult emergence,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sex determination; Chromosome elimination; Embryo; Insect.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100026

Ethylene and fruit ripening: from illumination gas to the control of gene expression, more than a century of discoveries
Provedor de dados: Genetics and Molecular Biology
Autores: Chaves,Ana Lúcia Soares; Mello-Farias,Paulo Celso de.
The effects of ethylene on plants have been recognized since the Nineteenth Century and it is widely known as the phytohormone responsible for fruit ripening and for its involvement in a number of plant growth and development processes. Elucidating the mechanisms involved in the ripening of climacteric fruit and the role that ethylene plays in this process have been central to fruit production and the improvement of fruit quality. The biochemistry, genetics and physiology of ripening has been extensively studied in economically important fruit crops and a considerable amount of information is available which ranges from the ethylene biosynthesis pathway to the mechanisms of perception, signaling and control of gene expression. However, there is still much...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Climacteric fruits; Plant hormones; Ethylene biosynthesis; Ripening; Signal transduction.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300020

The catalase gene family in cucumber: genome-wide identification and organization
Provedor de dados: Genetics and Molecular Biology
Autores: Hu,Lifang; Yang,Yingui; Jiang,Lunwei; Liu,Shiqiang.
Abstract Catalase (CAT) is a common antioxidant enzyme in almost all living organisms. Currently, detailed reports on cucumber (Cucumis sativus L.) CAT (CsCAT) genes and tissue expression profiling are limited. In the present study, four candidate CsCAT genes were identified in cucumber. Phylogenetic analysis indicated that CsCAT1-CsCAT3 are closely related to Arabidopsis AtCAT1-AtCAT3, but no obvious counterpart was observed for CsCAT4. Intron/exon structure analysis revealed that only one of the 15 positions was completely conserved. Motif analysis showed that, unlike the CAT genes of other species, none of CsCAT genes contained all 10 motifs. Expression data showed that transcripts of all of the CsCAT genes, except CsCAT4, were detected in five tissues....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cucumis sativus L.; Catalase; Phylogenetic analysis; Gene family; Motif.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300408

Genetic and epigenetic effects of salinity on in vitro growth of barley
Provedor de dados: Genetics and Molecular Biology
Autores: Demirkiran,Aykut; Marakli,Sevgi; Temel,Aslihan; Gozukirmizi,Nermin.
Morphological, physiological and molecular changes were investigated in in vitro salt-stressed barley (Hordeum vulgare L. cv. Tokak). Mature embryos were cultured in Murashige and Skoog medium containing 0 (control), 50 and 100 mM NaCl for 20 days. Both concentrations inhibited shoot growth, decreased fresh weight and protein content, and increased SOD (EC 1.15.1.1) activity in a dose-dependent manner. The lower concentration increased root growth. Salinity caused nucleotide variations in roots, but did not affect shoot DNAs. The higher concentration caused methylation changes, mainly hypermethylation in shoots. This is the first study on genetic and epigenetic effects of salinity in barley.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hordeum vulgare; Salt stress; Tissue culture; RAPD; CRED-RA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400016

Development of two sequence-specific PCR markers linked to the le gene that reduces pod shattering in narrow-leafed Lupin (Lupinus angustifolius L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Boersma,Jeffrey G.; Buirchell,Bevan J.; Sivasithamparam,Krishnapillai; Yang,Huaan.
Wild types of narrow-leaf lupin (Lupinus angustifolius L.) have seed pods that shatter upon maturity, leading to the loss of their seeds before or during the harvest process. Two recessive genes have been incorporated into domesticated cultivars of this species to maximize harvest-ability of the produce. One of these genes is called lentus (le). Two microsatellite - anchored fragment length polymorphism (MFLP) candidate markers were identified as closely linked to the le gene in a recombinant inbred line (RIL) population derived from a domesticated x wild type cross. The candidate MFLP markers were isolated from the gel, re-amplified by PCR, cloned and sequenced. The MFLP polymorphisms were converted into sequence-specific PCR-based markers. Linkage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pod shattering; Lupin; MFLP; Molecular marker; Marker assisted selection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400020

ABC transporters in Mycoplasma hyopneumoniae and Mycoplasma synoviae: insights into evolution and pathogenicity
Provedor de dados: Genetics and Molecular Biology
Autores: Nicolás,Marisa Fabiana; Barcellos,Fernando Gomes; Nehab Hess,Pablo; Hungria,Mariangela.
ABC transporters represent one of the largest superfamilies of active membrane transport proteins (MTPs) with a highly conserved ATPase domain that binds and hydrolyzes ATP, supplying energy for the uptake of a variety of nutrients and for the extrusion of drugs and metabolic wastes. The complete genomes of a non-pathogenic (J) and pathogenic (7448) strain of Mycoplasma hyopneumoniae, as well as of a pathogenic (53) strain of Mycoplasma synoviae have been recently sequenced. A detailed study revealed a high percentage of CDSs encoding MTPs in M. hyopneumoniae strains J (13.4%), 7448 (13.8%), and in M. synoviae 53 (11.2%), and the ABC systems represented from 85.0 to 88.6% of those CDSs. Uptake systems are mainly involved in cell nutrition and some might be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ABC transporters; Bioinformatics; Genomes of microorganisms; Membrane transport proteins; Mycoplasma; Phylogeny of prokaryotes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200006

Mitochondrial genetic variability of Didelphis albiventris (Didelphimorphia, Didelphidae) in Brazilian localities
Provedor de dados: Genetics and Molecular Biology
Autores: Sousa,Luciene C.C.; Gontijo,Célia M.F.; Botelho,Helbert A.; Fonseca,Cleusa G.
Didelphis albiventris is a well-known and common marsupial. Due to its high adaptability, this very widespread generalist species occurs under various environmental conditions, this even including protected regions and disturbed urban areas. We studied a 653 bp fragment of cytochrome oxidase c (COI) from 93 biological samples from seven Brazilian localities, with linear distances ranging between 58 and about 1800 km to analyze the effects of geographic distances on variability and genetic differentiation. The haplotype network presented nine haplotypes and two genetic clusters compatible with the two most distant geographic areas of the states of Minas Gerais, in the southeast, and Rio Grande do Sul, in the extreme south. As each cluster was characterized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Didelphis albiventris; Marsupial; Variability; COI; Genetic differentiation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300020

Analysis of genetic diversity and population structure in Argentine and Bolivian Creole cattle using five loci related to milk production
Provedor de dados: Genetics and Molecular Biology
Autores: Lirón,J.P.; Ripoli,M.V.; De Luca,J.C.; Peral-García,P.; Giovambattista,G.
Data from five protein-coding loci related to dairy production were used to study the genetic diversity and population structure of Argentine and Bolivian Creole cattle breeds. Genomic DNA was extracted from blood samples of six Creole cattle breeds: Argentine (n = 230), Patagonian (n = 25); "Saavedreño" (n = 140), "Chaqueño Boliviano" (n = 30), "Yacumeño" (n = 27), and "Chusco" (n = 11). kappa-casein, beta-lactoglobulin, growth hormone and prolactin were measured by PCR-RFLP, while alphaS1-casein was typed by PCR-ASO. The results are discussed, focusing on: historical origin, recent differentiation and selection events, Zebu gene introgression, and population structure. This work shows that: (i) For the studied genes, the observed gene frequency profiles...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Creole cattle; Polymorphism; Genetic diversity; Molecular markers.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400010

Cloning and characterization of endo-β-1,4-glucanase genes in the common wheat line three pistils
Provedor de dados: Genetics and Molecular Biology
Autores: Yang,Zaijun; Peng,Zhengsong; Wei,Shuhong; Yu,Yan.
In this work, we report the cloning and characterization of endo-β-1,4-glucanase (EGase) genes (TaEG) in the common wheat line three pistils. Three TaEG homoeologous genes (TaEG-4A, TaEG-4B and TaEG-4D) were isolated and found to be located on chromosomes 4AL, 4BS and 4DS, respectively. The three genes showed high conservation of their coding nucleotide sequences and 3 untranslated region. The putative TaEG protein had a molecular mass of 69 kDa, a theoretical pI of 9.39 and a transmembrane domain of 74-96 amino acids in the N-terminus that anchored the protein to the membrane. The genome sequences of TaEG-4A, TaEG-4B and TaEG-4D contained six exons and five introns. All of the introns, except for intron IV, varied in length and sequence composition....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cloning endo-β-1; 4-glucanase three pistils line wheat.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300015

Molecular characterization and transcription analysis of DNA methyltransferase genes in tomato (Solanum lycopersicum)
Provedor de dados: Genetics and Molecular Biology
Autores: Guo,Xuhu; Xie,Qian; Li,Baoyuan; Su,Huanzhen.
Abstract DNA methylation plays an important role in plant growth and development, gene expression regulation, and maintenance of genome stability. However, only little information regarding stress-related DNA methyltransferases (MTases) genes is available in tomato. Here, we report the analysis of nine tomato MTases, which were categorized into four known subfamilies. Structural analysis suggested their DNA methylase domains are highly conserved, whereas the N-terminals are divergent. Tissue-specific analysis of these MTase genes revealed that SlCMT2, SlCMT3, and SlDRM5 were expressed higher in young leaves, while SlMET1, SlCMT4, SlDRM7, and SlDRM8 were highly expressed in immature green fruit, and their expression declined continuously with further fruit...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA methylation; Tissue-specific expression; Abiotic stress; Fruit ripening; Tomato.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100307

Gene expression analysis reveals important pathways for drought response in leaves and roots of a wheat cultivar adapted to rainfed cropping in the Cerrado biome
Provedor de dados: Genetics and Molecular Biology
Autores: Poersch-Bortolon,Liane Balvedi; Pereira,Jorge Fernando; Nhani Junior,Antonio; Gonzáles,Hebert Hernán Soto; Torres,Gisele Abigail Montan; Consoli,Luciano; Arenhart,Rafael Augusto; Bodanese-Zanettini,Maria Helena; Margis-Pinheiro,Márcia.
Abstract Drought limits wheat production in the Brazilian Cerrado biome. In order to search for candidate genes associated to the response to water deficit, we analyzed the gene expression profiles, under severe drought stress, in roots and leaves of the cultivar MGS1 Aliança, a well-adapted cultivar to the Cerrado. A set of 4,422 candidate genes was found in roots and leaves. The number of down-regulated transcripts in roots was higher than the up-regulated transcripts, while the opposite occurred in leaves. The number of common transcripts between the two tissues was 1,249, while 2,124 were specific to roots and 1,049 specific to leaves. Quantitative RT-PCR analysis revealed a 0.78 correlation with the expression data. The candidate genes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 454 sequencing; Candidate genes; RT-qPCR; Triticum aestivum; Water deficit.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400629

Microbial quality of soil from the Pampa biome in response to different grazing pressures
Provedor de dados: Genetics and Molecular Biology
Autores: Vargas,Rafael S.; Bataiolli,Renata; da Costa,Pedro B.; Lisboa,Bruno; Passaglia,Luciane Maria P.; Beneduzi,Anelise; Vargas,Luciano K.
The aim of this study was to evaluate the impact of different grazing pressures on the activity and diversity of soil bacteria. We performed a long-term experiment in Eldorado do Sul, southern Brazil, that assessed three levels of grazing pressure: high pressure (HP), with 4% herbage allowance (HA), moderate pressure (MP), with 12% HA, and low pressure (LP), with 16% HA. Two reference areas were also assessed, one of never-grazed native vegetation (NG) and another of regenerated vegetation after two years of grazing (RG). Soil samples were evaluated for microbial biomass and enzymatic (β-glucosidase, arylsulfatase and urease) activities. The structure of the bacterial community and the population of diazotrophic bacteria were evaluated by RFLP of the 16S...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial diversity; Diazotrophic bacteria; Diversity; Grasslands; Soil microbial communities.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200205

The historical biogeography of  Pteroglossus  aracaris (Aves, Piciformes, Ramphastidae) based on Bayesian analysis of mitochondrial DNA sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Sérgio L.; Wajntal,Anita.
Most Neotropical birds, including  Pteroglossus  aracaris, do not have an adequate fossil record to be used as time constraints in molecular dating. Hence, the evolutionary timeframe of the avian biota can only be inferred using alternative time constraints. We applied a Bayesian relaxed clock approach to propose an alternative interpretation for the historical biogeography of  Pteroglossus  based on mitochondrial DNA sequences, using different combinations of outgroups and time constraints obtained from outgroup fossils, vicariant barriers and molecular time estimates. The results indicated that outgroup choice has little effect on the Bayesian posterior distribution of divergence times within  Pteroglossus , that geological and molecular time constraints...
Palavras-chave: Pteroglossus; Ramphastidae; Aves; Molecular time estimates; Biogeography.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500026

Xylella fastidiosa comparative genomic database is an information resource to explore the annotation, genomic features, and biology of different strains
Provedor de dados: Genetics and Molecular Biology
Autores: Varani,Alessandro M.; Monteiro-Vitorello,Claudia B.; Almeida,Luiz G.P. de; Souza,Rangel C.; Cunha,Oberdan L.; Lima,Wanessa C.; Civerolo,Edwin; Sluys,Marie-Anne Van; Vasconcelos,Ana T.R.
The Xylella fastidiosa comparative genomic database is a scientific resource with the aim to provide a user-friendly interface for accessing high-quality manually curated genomic annotation and comparative sequence analysis, as well as for identifying and mapping prophage-like elements, a marked feature of Xylella genomes. Here we describe a database and tools for exploring the biology of this important plant pathogen. The hallmarks of this database are the high quality genomic annotation, the functional and comparative genomic analysis and the identification and mapping of prophage-like elements. It is available from web site http://www.xylella.lncc.br.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genome annotation and assembly; Comparative genomics; Mobile genetic elements.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100021

Description of a ZZ/ZW sex chromosome system in Thoracocharax cf. stellatus (Teleostei, Characiformes, Gasteropelecidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Margarida Lima; Oliveira,Claudio; Foresti,Fausto.
The family Gasteropelecidae is composed of three genera and eight species. This study shows that Thoracocharax cf. stellatus has 2n = 52 chromosomes for both sexes. The five males studied showed 8 metacentric, 16 submetacentric, 4 subtelocentric, and 24 acrocentric chromosomes; the seven females showed only one submetacentric chromosome, belonging to pair 11, and one extra acrocentric chromosome, smaller than all the other chromosomes, characterizing the presence of a ZZ/ZW sex chromosome system in this species. Nucleolus organizing regions (NORs) were detected on the short arms of the subtelocentric chromosome pair 13. Constitutive heterochromatin was identified at pericentromeric and terminal positions in almost all chromosomes. The W chromosome was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sex chromosomes; ZW; DNA content; Fish; Evolution.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300008

Cooperation and interplay between base and nucleotide excision repair pathways: From DNA lesions to proteins
Provedor de dados: Genetics and Molecular Biology
Autores: Kumar,Namrata; Moreno,Natália C.; Feltes,Bruno C.; Menck,Carlos FM; Houten,Bennett Van.
Abstract Base and nucleotide excision repair (BER and NER) pathways are normally associated with removal of specific types of DNA damage: small base modifications (such as those induced by DNA oxidation) and bulky DNA lesions (such as those induced by ultraviolet or chemical carcinogens), respectively. However, growing evidence indicates that this scenario is much more complex and these pathways exchange proteins and cooperate with each other in the repair of specific lesions. In this review, we highlight studies discussing the involvement of NER in the repair of DNA damage induced by oxidative stress, and BER participating in the removal of bulky adducts on DNA. Adding to this complexity, UVA light experiments revealed that oxidative stress also causes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Base excision repair; Nucleotide excision repair; DNA damage; Protein oxidation; UVA light.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200309

Genetic variability in geographical populations of Aedes aegypti (Diptera, Culicidae) in Brazil elucidated by molecular markers
Provedor de dados: Genetics and Molecular Biology
Autores: Paduan,Karina dos Santos; Araújo-Júnior,João P.; Ribolla,Paulo E.M.
The tropical mosquito, Aedes aegypti is the most important domestic vector of urban yellow fever and dengue. Genetic population studies on this vector are important because they may lead to new tools for surveillance. An analysis of genetic structure was conducted among populations of A. aegypti from 11 localities in four demographic regions within six Brazilian federal states. Markers included 21 random amplified polymorphic DNA (RAPD) loci. RAPD markers were detected among populations and cluster analysis revealed two main groups. We found high genetic polymorphism (H S = 0.224) and high levels of genetic differentiation between populations from different states (G ST = 0.430), as well as in populations from cities in the same state (G ST = 0.410). These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aedes aegypti; Population genetics; Random amplified polymorphic DNA; Genetic variability.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200030

Acetosyringone, pH and temperature effects on transient genetic transformation of immature embryos of Brazilian wheat genotypes by Agrobacterium tumefaciens
Provedor de dados: Genetics and Molecular Biology
Autores: Manfroi,Ernandes; Yamazaki-Lau,Elene; Grando,Magali F.; Roesler,Eduardo A.
Abstract Low transformation efficiency is one of the main limiting factors in the establishment of genetic transformation of wheat via Agrobacterium tumefaciens. To determine more favorable conditions for T-DNA delivery and explant regeneration after infection, this study investigated combinations of acetosyringone concentration and pH variation in the inoculation and co-cultivation media and co-culture temperatures using immature embryos from two Brazilian genotypes (BR 18 Terena and PF 020037). Based on transient expression of uidA, the most favorable conditions for T-DNA delivery were culture media with pH 5.0 and 5.4 combined with co-culture temperatures of 22 °C and 25 °C, and a 400 μM acetosyringone supplement. These conditions resulted in blue foci...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Co-cultivation conditions; Protocol establishment; GUS; T-DNA delivery.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400470

Aspects of gene regulation in the diploid and tetraploid Odontophrynus americanus (Amphibia, Anura, Leptodactylidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Cianciarullo,Aurora M.; Naoum,Paulo C.; Bertho,Álvaro L.; Kobashi,Leonardo S.; Beçak,Willy; Soares,Maurilio J.
Erythropoietic and hemoglobin DNA transcriptional activities were analyzed in the diploid and the tetraploid Odontophrynus americanus. Flow cytometric analyses of DNA, RNA and mitochondrial contents showed increased genic activity in both diploid and tetraploid animals during erythropoiesis in vivo elicited by pretreatment phenylhydrazine. Generally, higher values were seen in immature tetraploid erythroid cells. On the 10th day of recovery from anemia, large amounts of messenger RNA were found in both specimens. Based on the mitochondrial content, the tetraploid cells had more intense energy metabolism than the diploid cells. Diploid O. americanus had about three times more erythroid cells than tetraploid specimens, indicating that there were differences...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200020

Expression profiles of sugarcane under drought conditions: Variation in gene regulation
Provedor de dados: Genetics and Molecular Biology
Autores: Andrade,Júlio César Farias de; Terto,Jackeline; Silva,José Vieira; Almeida,Cícero.
Abstract Drought is a major factor in decreased sugarcane productivity because of the resulting morphophysiological effects that it causes. Gene expression studies that have examined the influence of water stress in sugarcane have yielded divergent results, indicating the absence of a fixed pattern of changes in gene expression. In this work, we investigated the expression profiles of 12 genes in the leaves of a drought-tolerant genotype (RB72910) of sugarcane and compared the results with those of other studies. The genotype was subjected to 80–100% water availability (control condition) and 0–20% water availability (simulated drought). To analyze the physiological status, the SPAD index, Fv/Fm ratio, net photosynthesis (A), stomatal conductance (gs) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; Plant breeding; Quantitative RT-PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400465

The Development of the Study of Hereditary Cancer in South America
Provedor de dados: Genetics and Molecular Biology
Autores: Rossi,Benedito Mauro; Sarroca,Carlos; Vaccaro,Carlos; Lopez,Francisco; Ashton-Prolla,Patricia; Ferreira,Fabio de Oliveira; Santos,Erika Maria Monteiro.
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200166

Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Jéssica de; Tureck,Luciane Viater; Santos,Willian dos; Saliba,Louise Farah; Schenknecht,Caroline Schovanz; Scaraboto,Débora; Souza,Ricardo Lehtonen R.; Furtado-Alle,Lupe.
Abstract Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G &gt; A (rs1126680), 1615GA (rs1803274), 1914A &lt; G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI &lt; 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BCHE gene; Obesity; Lipid metabolism; Polymorphisms.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300408

The gene expression profiles of induced pluripotent stem cells (iPSCs) generated by a non-integrating method are more similar to embryonic stem cells than those of iPSCs generated by an integrating method
Provedor de dados: Genetics and Molecular Biology
Autores: Liu,Yajun; Cheng,De; Li,Zhenzhen; Gao,Xing; Wang,Huayan.
Induced pluripotent stem cells (iPSCs) obtained by the ectopic expression of defined transcription factors have tremendous promise and therapeutic potential for regenerative medicine. Many studies have highlighted important differences between iPSCs and embryonic stem cells (ESCs). In this work, we used meta-analysis to compare the global transcriptional profiles of human iPSCs from various cellular origins and induced by different methods. The induction strategy affected the quality of iPSCs in terms of transcriptional signatures. The iPSCs generated by non-integrating methods were closer to ESCs in terms of transcriptional distance than iPSCs generated by integrating methods. Several pathways that could be potentially useful for studying the molecular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA microarray analysis; Embryonic stem cells; Gene expression profiling; Induced pluripotent stem cells.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400022

Effect of bacterial recA expression on DNA repair in the rad51 and rad52 mutants of Saccharomyces cerevisiae
Provedor de dados: Genetics and Molecular Biology
Autores: Morais Jr.,M.A.; Vlcková,V.; Fridrichová,I.; Slaninová,M.; Brozmanová,J.; Henriques,J.A.P.
Molecular and functional homology between yeast proteins pRad51 and pRad52 and Escherichia coli pRecA involved in recombinational DNA repair led us to investigate possible effects of recA gene expression on DNA repair in rad51 and rad52 mutants of Saccharomyces cerevisiae. The mutant cells were subjected to one of the following treatments: preincubation with 8-methoxypsoralen and subsequent irradiation with 360-nm ultraviolet (UVA) (8-MOP + UVA), irradiation with 254-nm UV light or treatment with methyl methane sulfonate (MMS). While recA expression did not repair lethal DNA lesions in mutant rad51, it was able to partially restore resistance to 8-MOP + UVA and MMS in rad52. Expression of recA could not complement the sensitivity of rad51rad52 double...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100002

Production of recombinant proteins in Escherichia coli
Provedor de dados: Genetics and Molecular Biology
Autores: Schumann,Wolfgang; Ferreira,Luis Carlos S.
Attempts to obtain a recombinant protein using prokaryotic expression systems can go from a rewarding and rather fast procedure to a frustrating time-consuming experience. In most cases production of heterologous proteins in Escherichia coli K12 strains has remained an empirical exercise in which different systems are tested without a careful insight into the various factors affecting adequate expression of the encoded protein. The present review will deal with E. coli as protein factory and will cover some of the aspects related to transcriptional and translational expression signals, factors affecting protein stability and solubility and targeting of proteins to different cell compartments. Based on the knowledge accumulated over the last decade, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Expression vectors; Secretion; Molecular chaperones.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300022

New molecular evidence supports the species status of Kaempfer's Woodpecker (Aves, Picidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Azevedo,Lorena de Sousa; Aleixo,Alexandre; Santos,Marcos Pérsio Dantas; Sampaio,Iracilda; Schneider,Horacio; Vallinoto,Marcelo; Rêgo,Péricles Sena do.
Kaempfer's Woodpecker (Celeus obrieni) is the only species of the genus Celeus endemic to Brazil. The description of this taxon as a subspecies of the Rufous-headed Woodpecker (Celeus spectabilis) was based on a single specimen. While C. obrieni and C. spectabilis are now considered separate species based on morphological and limited molecular evidence, no study has critically tested the reciprocal monophyly and degree of evolutionary independence between these taxa with several specimens. Herein, fragments of the mitochondrial and nuclear DNA of three recently-collected specimens of C. obrieni were analyzed to evaluate the degree of evolutionary differentiation of this taxon with respect to C. spectabilis. The results confirm the reciprocal monophyly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bamboo; Biogeography; Celeus obrieni; Celeus spectabilis; Taxonomy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200009

Three decades of studies on chromosomal polymorphism of Drosophila willistoni and description of fifty different rearrangements
Provedor de dados: Genetics and Molecular Biology
Autores: Rohde,Claudia; Valente,Vera Lúcia S.
Drosophila willistoni (Insecta, Diptera) is considered a paradigm for evolutionary studies. Their chromosomes are characterized by multiple paracentric inversions that make it hard to identify and describe chromosomal poly-morphisms. In the present report we attempted to systematize the description of all the 50 inversions found in the last three decades, since we have been studying the chromosomes of several individuals of 30 different populations, including the one used in the genome sequencing project (Gd-H4-1). We present the photographic register of 11 arrangements in the left arm of the X chromosome (XL), eight in the right arm (XR), 10 in the left arm of chromosome II (IIL), eight in its right arm (IIR) and 13 in chromosome III. This information...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetic; Polytene chromosomes; Inversions; Drosophila willistoni.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600012

Genetic polymorphism of milk protein loci in Argentinian Holstein cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Bonvillani,Adriana Gloria; Di Renzo,Miguel Angel; Tiranti,Iván Nicolás.
Some alleles of milk protein loci are associated with superior cheese production characteristics. The genetic polymorphism of the milk protein loci alphas1-casein, beta-casein, k-casein and beta-lactoglobulin was examined in Argentinian Holstein cattle. Samples from 12 herds of four regions of Córdoba were analyzed by starch gel electrophoresis. The chi² test was used to assess whether the populations were in Hardy-Weinberg equilibrium. Genotypic diversity was analyzed by the Shannon-Weaver index. The observed genotypic frequencies were analyzed by Hedrick's genetic identity and the genetic distance of Balakrishnan and Sanghvi. The allelic and genotypic frequencies were similar to those of other Holstein populations. The genotypic frequencies of the...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400019

Characterization and genetic diversity analysis of cotton cultivars using microsatellites
Provedor de dados: Genetics and Molecular Biology
Autores: Bertini,Cândida H.C. de Magalhães; Schuster,Ivan; Sediyama,Tocio; Barros,Everaldo Gonçalves de; Moreira,Maurílio Alves.
Genetic diversity and the relationship between varieties are of great importance for cotton breeding. Our work was designed to estimate the informativeness of the cotton (Gossypium hirsutum L.) simple sequence repeat (SSR) microsatellite locus and to estimate the genetic distance between 53 cotton cultivars as well as to select a set of SSR primers able to differentiate between the 53 cotton cultivars studied. After extracting DNA from the 53 cultivars and characterized it using 31 pairs of SSR primers we obtained a total of 66 alleles with an average of 2.13 alleles per SSR locus and values of polymorphism information content (PIC) varying from 0.18 to 0.62, the dissimilarity coefficient varying from zero to 0.41. Statistical analysis using the unweighted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fingerprinting; Gossypium hirsutum L.; Genealogy; Molecular markers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200021

Karyotype similarity between two sympatric Schizodon fish species (Anostomidae, Characiformes) from the Paraguay River basin
Provedor de dados: Genetics and Molecular Biology
Autores: Martins,Cesar; Galetti Jr.,Pedro Manoel.
Fish of the neotropical family Anostomidae generally show low karyotype variability. Nevertheless, karyotype variants have been identified within some genera, providing information about their evolutionary history. Species of the genus Schizodon show a high degree of morphological and ecological similarity compared to other anostomids. In the present study, karyotype characteristics of Schizodon borelli (40 individuals) and S. isognathum (one individual), two sympatric species found in the Paraguay River basin, were studied. C-banding, GC-specific fluorochrome Mitramycin (MM) and Ag staining as well as in situ hybridization (FISH) with rDNA probes were used. The karyotypes of these species were found to be very similar. Only two NORs were detected in a...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300011

Editorial
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100001

Variation in the mitochondrial control region sequence between the two maternal lines of the Sorraia horse breed
Provedor de dados: Genetics and Molecular Biology
Autores: Luís,Cristina; Bastos-Silveira,Cristiane; Cothran,E.Gus; Oom,M. Mar.
The mitochondrial control region sequence was compared between the two surviving matrilineal lineages of the Sorraia horse. Two haplotypes were found, showing three differences between them. The reduced number of surviving maternal lineages emphasizes the importance of establishing a conservation plan for this endangered breed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sorraia horse; Mitochondrial DNA; Maternal lineages; Control region.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300010

Widening the clinical spectrum of Pitt-Rogers-Danks/Wolf-Hirschhorn syndromes
Provedor de dados: Genetics and Molecular Biology
Autores: Mazzeu,Juliana F.; Krepischi-Santos,Ana Cristina; Rosenberg,Carla; Lourenço,Charles M.; Lezirovitz,Karina; Szuhai,Karoly; Martelli,Lúcia R.; Vianna-Morgante,Angela M.
Chromosomal rearrangements involving partial deletion of the short arm of chromosome 4 and partial duplication of the short arm of chromosome 8 have been described both in Pitt-Rogers-Danks syndrome (PRDS) and Wolf-Hirschhorn syndrome (WHS), the former being considered a milder phenotype of the latter. We describe a patient with partial deletion of chromosome 4 and partial duplication of chromosome 8 documented by array-comparative genomic hybridization (Array-CGH). In addition to the typical features of PRDS, the patient exhibited some clinical signs (genital hypoplasia, radioulnar synostosis and mesomelic limb shortness) infrequently, or never previously, reported in PRDS. These findings broaden the spectrum of anomalies generally associated with these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pitt-Rogers-Danks syndrome Robinow syndrome translocation t(4; 8) Wolf-Hirschhorn syndrome.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300007

Microsatellite studies on an isolated population of African descent in the Brazilian state of Bahia
Provedor de dados: Genetics and Molecular Biology
Autores: Barbosa,Ana Angélica Leal; Sousa,Sandra Mara Bispo; Abé-Sandes,Kiyoko; Alonso,Carlos Alberto; Schneider,Vicente; Costa,Denise C.C.; Cavalli,Iglenir João; Azevêdo,Eliane Elisa Souza.
The African descent population of the Bananal community in the Brazilian state of Bahia (BA) was characterized as a genetic isolate and analyzed for some short tandem repeat (STR) microsatellite autosomic polymorphic loci (CSF1PO, TH01, TPOX, F13A1, FESFPS and vWA). These genetic variants were further compared to data obtained from an urban sample from the town of Jequié (BA) regarding demographic and anthropogenetic aspects. The Bananal sample comprised 32 unrelated individuals whereas Jequié was represented by 76 individuals. The Bananal Negroid Phenotypic Index (NPI) was 0.98 and the Negroid Cultural Index (NCI) 0.24. Consanguineous marriages occurred at a frequency of 34.61% and the F value was 0.0126. All six loci studied were in Hardy-Weinberg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite; African-descendant isolate.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100004

A comparison of the effects of physical and chemical mutagens in sesame (Sesamum indicum L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Begum,Tamina; Dasgupta,Tapash.
Three sesame genotypes (Rama, SI 1666 and IC 21706) were treated with physical (γ-rays: 200 Gy, 400 Gy or 600 Gy) or chemical (ethyl methane sulphonate, EMS: 0.5%, 1.0%, 1.5% or 2.0%) mutagens and their mutagenic effectiveness and efficiency were estimated in the M2 generation. The M3 generation was used to identify the most effective mutagen and dose for induction of mutations. The average effectiveness of EMS was much higher than γ-rays. The lowest dose of γ-rays (200 Gy) and the lowest concentration of EMS (0.5%) showed the highest mutagenic efficiency in all genotypes. Analysis of the M3 generation data based on parameters such as the variance ratio and the difference in residual variances derived from the model of Montalván and Ando indicated that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Effectiveness; Efficiency; Mutagens; Quantitative characters; Sesame.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400025

Soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes): isolated isoforms and kinetics properties
Provedor de dados: Genetics and Molecular Biology
Autores: Aquino-Silva,Maria Regina de; Schwantes,Maria Luiza Barcellos; Munin,Flavia Simone; Schwantes,Arno Rudi; Santos,Silvana Pereira dos.
Kinetic properties and thermal stabilities of Geophagus brasiliensis skeletal muscle unfractionated malate dehydrogenase (MDH, EC 1.1.1.37) and its isolated isoforms were analyzed to examine a possible sMDH-B* locus duplication in a fixation process influenced by genetic drift. Two optimal pHs were detected: 7.5 for AB1 unfractionated muscle phenotype and its B1 isoform, and 8.0 for AB1B2 unfractionated muscle phenotype, A and B2 isoforms. While G. brasiliensis A isoform could be characterized as thermostable, the duplicated B isoform cannot be assumed as thermolabile. Km values for isolated B2 isoforms were 1.6 times lower than for B1. A duplication event in progress best explains the electrophoretic six-band pattern detected in G. brasiliensis, which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene duplication; SMDH; Substrate concentration; Temperature; PH isoforms.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200029

Adaptive response: some underlying mechanisms and open questions
Provedor de dados: Genetics and Molecular Biology
Autores: Dimova,Evgeniya G.; Bryant,Peter E.; Chankova,Stephka G.
Organisms are affected by different DNA damaging agents naturally present in the environment or released as a result of human activity. Many defense mechanisms have evolved in organisms to minimize genotoxic damage. One of them is induced radioresistance or adaptive response. The adaptive response could be considered as a nonspecific phenomenon in which exposure to minimal stress could result in increased resistance to higher levels of the same or to other types of stress some hours later. A better understanding of the molecular mechanism underlying the adaptive response may lead to an improvement of cancer treatment, risk assessment and risk management strategies, radiation protection, e.g. of astronauts during long-term space flights. In this mini-review...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adaptive response; Oxidative stress; DNA repair up-regulation; DNA-binding proteins; Antioxidant defense system.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300002

Characterization and chromosomal mapping of the DgmarMITE transposon in populations of Dichotomius (Luederwaldtinia) sericeus species complex (Coleoptera: Scarabaeidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Amorim,Igor Costa; Costa,Rafaelle Grazielle Coelho; Xavier,Crislaine; Moura,Rita de Cássia de.
Abstract Transposable elements are dispersed repetitive DNA sequences that can move within the genome and are related to genome and chromosome evolution, adaptation, and speciation. The aim of this study was to characterize and determine the chromosomal location and accumulation of a Mariner-like element in populations of four phylogenetically related species of the Dichotomius (Luederwaldtinia) sericeus complex. Mapping of the isolated element was performed by fluorescent in situ hybridization in different populations of analyzed species. Characterization of the isolated element revealed a degenerated transposon, named DgmarMITE. This transposon is 496-bp-long, AT rich (57%), and contains 24 bp terminal inverted repeats. In situ mapping revealed presence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mariner-like elements; Cross-mobilization; Chromosome evolution.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300419

Genome size of three Brazilian flies from the Sciaridae family
Provedor de dados: Genetics and Molecular Biology
Autores: Saccuti,Cecília Ferreira; Soares,Maria Albertina de Miranda; Falco,José Ricardo Penteado; Fernandez,Maria Aparecida.
We determined the genome size of the Brazilian sciarid flies Bradysia hygida, Rhynchosciara americana and Trichosia pubescens (Diptera: Sciaridae) using absorbance measurements of Feulgen-stained nuclei belonging to these species (and chicken erythrocytes as a standard) to calculate the amount of DNA in picograms (pg) and the number of base pairs (bp), or C-value, for each of these species. The C-values were: 3 x 10(8) bp (0.31 pg) for B. hygida; 3.6 x 10(8) bp (0.37 pg) for R. americana; and 1 x 10(9) bp (1.03 pg) for T. pubescens. The sciarids investigated in this work had considerably higher C-values than the average for previously described dipteran species, including D. melanogaster.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Genome size; Feulgen reaction; Sciaridae; Bradysia hygida; Rhynchosciara americana; Trichosia pubescens; Brazilian flies.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500015

Genetic evaluation for persistency of lactation in Holstein cows using a random regression model
Provedor de dados: Genetics and Molecular Biology
Autores: Cobuci,Jaime Araujo; Euclydes,Ricardo Frederico; Costa,Claudio Napolis; Torres,Robledo de Almeida; Lopes,Paulo Sávio; Pereira,Carmen Silva.
A model for analyzing test day records including both fixed and random coefficients was applied to the genetic evaluation of first lactation data for Holstein cows. Data comprising 87045 test-day milk yield records from calving between 1997 and 2001 from Holstein herds in 10 regions of the Brazilian state of Minas Gerais. Six persistency of lactation measures were evaluated using breeding values obtained by random regression analyses. The Wilmink function was used to model the additive genetic and permanent environmental effects. Residual variance was constant throughout lactation. Ranking for animals did not change among criteria for persistency measurements, but ranking changes were observed when the estimated breeding value (EBV) for persistency of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic evaluation; Persistency of lactation; Rank correlation; Random regression models.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300009

Chromosomal localization and molecular marker development of the lipopolysaccharide and beta-1,3-glucan binding protein gene in the Zhikong scallop Chlamys farreri (Jones et Preston) (Pectinoida, Pectinidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Huan,Pin; Zhang,Xiaojun; Li,Fuhua; Zhang,Yang; Zhao,Cui; Xiang,Jianhai.
Zhikong scallop Chlamys farreri (Jones et Preston) is an economically important species in China. Understanding its immune system would be of great help in controlling diseases. In the present study, an important immunity-related gene, the Lipopolysaccharide and Beta-1,3-glucan Binding Protein (LGBP) gene, was located on C. farreri chromosomes by mapping several lgbp-containing BAC clones through fluorescence in situ hybridization (FISH). Through the localization of various BAC clones, it was shown that only one locus of this gene existed in the genome of C. farreri, and that this was located on the long arm of a pair of homologous chromosomes. Molecular markers, consisting of eight single nucleotide polymorphism (SNPs) markers and one insertion-deletion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlamys farreri; LGBP; Fluorescence in situ hybridization (FISH); Indel; SNP.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100008

Comparative mapping reveals quantitative trait loci that affect spawning time in coho salmon (Oncorhynchus kisutch)
Provedor de dados: Genetics and Molecular Biology
Autores: Araneda,Cristian; Díaz,Nelson F.; Gomez,Gilda; López,María Eugenia; Iturra,Patricia.
Spawning time in salmonids is a sex-limited quantitative trait that can be modified by selection. In rainbow trout (Oncorhynchus mykiss), various quantitative trait loci (QTL) that affect the expression of this trait have been discovered. In this study, we describe four microsatellite loci associated with two possible spawning time QTL regions in coho salmon (Oncorhynchus kisutch). The four loci were identified in females from two populations (early and late spawners) produced by divergent selection from the same base population. Three of the loci (OmyFGT34TUF, One2ASC and One19ASC) that were strongly associated with spawning time in coho salmon (p &lt; 0.0002) were previously associated with QTL for the same trait in rainbow trout; a fourth loci (Oki10)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coho salmon; QTL; Spawning time.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300019

Simultaneous overexpression of GH and STAT5b genes inhibits the STAT5 signalling pathway in tilapia (Oreochromis niloticus) embryos
Provedor de dados: Genetics and Molecular Biology
Autores: Marins,Luis Fernando; Iyengar,Arati; Maclean,Norman; Levy,Jose A.; Sohm,Frédéric.
In this study, we describe the use of a STAT5 responsive element (LHRE) reporter gene to monitor the activity of the growth hormone (GH) transduction pathway following expression of heterologous fish GH and rat STAT5b in tilapia embryos and fish fibroblast cells. Our results indicate that both GH and STAT5b are able to activate the LHRE at high levels when transferred separately, demonstrating the substantial level of conservation of the GH signal transduction pathways between fish and mammals. Unexpectedly, co-expression experiments show a strong inhibition of the GH-dependent activation, suggesting that simultaneous GH and STAT5b overexpression can counteract effects of GH expression in tilapia embryos.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Teleost; Growth hormone; Signal transduction; Cytokine; Trangenesis signal transducers and activators of transcription.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300007

Protective effects of solvent fractions of Mentha spicata (L.) leaves evaluated on 4-nitroquinoline-1-oxide induced chromosome damage and apoptosis in mouse bone marrow cells
Provedor de dados: Genetics and Molecular Biology
Autores: Arumugam,Ponnan; Ramesh,Arabandi.
Spearmint leaves (Mentha spicata L.) contain high levels of antioxidants that are known to protect against both exogenous and endogenous DNA damage. In this study, the protective effects of the hexane fraction (HF), chloroform fraction (CF) and ethyl acetate fraction (EAF) in an ethanol extract from M. spicata were evaluated against 4-nitroquinoline-1-oxide (4-NQO) induced chromosome damage and apoptosis in bone marrow cells of Swiss albino mice. Two (EAF; 80 and 160 mg/ kg body weight - bw) or three (HF and CF; 80, 160 and 320 mg/ kg bw) doses of solvent fractions or vehicle control (25% DMSO in water) were administered orally for five consecutive days. Upon the sixth day, 4-NQO was injected intraperitoneally. The animals were killed the following day....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 4-NQO; Chromosome damage; Mentha spicata protective effects.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400028

Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines
Provedor de dados: Genetics and Molecular Biology
Autores: Yamada,Hiroya; Takano,Toru; Matsuzuka,Fumio; Watanabe,Mikio; Miyauchi,Akira; Iwatani,Yoshinori.
Thyroid transcription factor-1 (TTF-1, NKX2-1) is a homeodomain-containing transcriptional factor that binds to and activates the promoters of thyroid and lung-specific genes, such as thyroglobulin, thyroid peroxidase, and thyroid stimulating hormone receptor. TTF-1 is known to play a key role in the development of the thyroid. However, the precise mechanism of TTF-1 gene transcription in human thyroid cells has not been studied. The expression of transcriptional activity in various lengths of the 5'-flanking region of the human TTF -1 gene was studied in TTF-1 positive and negative human thyroid cell lines. Increased transcriptional activity was observed in thyroid cell lines containing plasmids that coded for a sequence proximal to the transcription...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid; TTF-1; Transcription; Human cell line; Stem cell.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100002

Modulatory effects of the antioxidant ascorbic acid on the direct genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster
Provedor de dados: Genetics and Molecular Biology
Autores: Fragiorge,Edson José; Spanó,Mário Antônio; Antunes,Lusânia Maria Greggi.
In this study two different crosses involving the wing cell markers mwh and flr³ (standard (ST) cross and high bioactivation (HB) cross, the latter being characterized by a high constitutive level of cytochrome P450 which leads to an increased sensitivity to a number of promutagens and procarcinogens) were used to investigate the modulatory effects of ascorbic acid (AA) combined with the antitumor agent doxorubicin (DXR) in Drosophila melanogaster. We observed that the two different concentrations of AA (50 or 100 mM) had no effect on spots frequencies, while DXR treatments (0.2 or 0.4 mM) gave positive results for all types of spots, when compared to negative control. For marker-heterozygous (MH) flies, a protective effect was observed with the lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascorbic acid; Doxorubicin; Drosophila melanogaster; Genotoxicity tests; Somatic mutation; Recombination test.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300025

Evidence that the Ceratobasidium-like white-thread blight and black rot fungal pathogens from persimmon and tea crops in the Brazilian Atlantic Forest agroecosystem are two distinct phylospecies
Provedor de dados: Genetics and Molecular Biology
Autores: Ceresini,Paulo C.; Costa-Souza,Elaine; Zala,Marcello; Furtado,Edson L.; Souza,Nilton L.
The white-thread blight and black rot (WTBR) caused by basidiomycetous fungi of the genus Ceratobasidium is emerging as an important plant disease in Brazil, particularly for crop species in the Ericales such as persimmon (Diospyros kaki) and tea (Camellia sinensis). However, the species identity of the fungal pathogen associated with either of these hosts is still unclear. In this work, we used sequence variation in the internal transcribed spacer regions, including the 5.8S coding region of rDNA (ITS-5.8S rDNA), to determine the phylogenetic placement of the local white-thread-blight-associated populations of Ceratobasidium sp. from persimmon and tea, in relation to Ceratobasidium species already described world-wide. The two sister populations of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecological speciation; Fungal plant pathogens; Host specialization; Population divergence; Phylogenetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300016

The association between adult-type hypolactasia and symptoms of functional dyspepsia
Provedor de dados: Genetics and Molecular Biology
Autores: Wortmann,André Castagna; Simon,Daniel; Mazzoleni,Luiz Edmundo; Sander,Guilherme Becker; Francesconi,Carlos Fernando de Magalhães; Nabinger,Débora Dreher; Grott,Camila Schultz; Rech,Tássia Flores; Mazzoleni,Felipe; Lunge,Vagner Ricardo; Bona,Laura Renata de; Milbradt,Tobias Cancian; Silveira,Themis Reverbel da.
Abstract Functional dyspepsia and lactose intolerance (adult-type hypolactasia, ATH) are common conditions that may coexist or even be confounded. Their clinical presentation can be similar, however, lactose intolerance does not form part of the diagnostic investigation of functional dyspepsia. Studies on the association between functional dyspepsia and ATH are scarce. This study aimed to evaluate whether ATH is associated with symptoms of functional dyspepsia. Patients fulfilling the Rome III diagnostic criteria for functional dyspepsia underwent genetic testing for ATH. Dyspeptic symptoms were evaluated and scored according to a validated questionnaire. The diagnostic criteria for ATH was a CC genotype for the -13910C/T polymorphism, located upstream of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bloating; Dyspepsia; Gastrointestinal diseases; Lactose intolerance; Single nucleotide polymorphism.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100092

Insertion sequences as variability generators in the Mycoplasma hyopneumoniae and M. synoviae genomes
Provedor de dados: Genetics and Molecular Biology
Autores: Loreto,Elgion Lúcio Silva; Ortiz,Mauro Freitas; Porto,Jorge Ivan Rebelo.
We have analyzed the sequenced genomes of three strains of Mycoplasma hyopneumoniae and one strain of M. synoviae, and have found three and two different transposable element families, respectively in each species. In M. hyopneumoniae, the Insertion Sequences of the IS4 family is represented by ISMHp1, a putatively active element. The IS3 family is represented by several degenerated sequences. A third element called tMH was found, which shows some characteristics reminiscent of retrotransposons. In M. synoviae, three different possibly active IS4 elements are present (ISMHp1-like; ISMs1 and IS1634-like elements). The IS30 family is represented by the degenerated IS1630-like element. The IS1634-like element is shown to be involved in chromosomal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insertion sequences; IS; Mollicutes; Horizontal gene transfer; Mycoplasma.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200017

Localization of genes modulating the predisposition to schizophrenia: a revision
Provedor de dados: Genetics and Molecular Biology
Autores: Lopes-Machado,E.Z.; Duarte,F.A.M.
The genetics of schizophrenia or bipolar affective disorder has advanced greatly at the molecular level since the introduction of probes for the localization of specific genes. Research on gene candidates for susceptibility to schizophrenia can broadly be divided into two types, i.e., linkage studies, where a gene is found near a specific DNA marker on a specific chromosome, and association studies, when a condition is associated with a specific allele of a specific gene. This review covers a decade of publications in this area, from the 1988 works of Bassett et al. and Sherrington et al. on a gene localized on the long arm of chromosome 5 at the 5q11-13 loci, to the 1997 work of Lin et al. pointing to the 13q14.1-q32 loci of chromosome 13 and to the 1998...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300009

Perforin gene PRF1 c.900C&amp;gt; T polymorphism and HIV-1 vertical transmission
Provedor de dados: Genetics and Molecular Biology
Autores: Zupin,Luisa; Polesello,Vania; Kamada,Anselmo Jiro; Gratton,Rossella; Segat,Ludovica; Kuhn,Louise; Crovella,Sergio.
Abstract Perforin-1, a component of the immune system, is able to control Human Immunodeficiency Virus-1 (HIV-1) replication and could be involved in HIV-1 mother-to-child transmission (MTCT). This study aims at evaluating the role of the c.900C &gt; T PRF1 gene (encoding for perforin-1) polymorphism (rs885822) in HIV-1 MTCT. The PRF1 c.900C &gt; T polymorphism was genotyped in 331 children from Zambia using a Taqman probe on a Real Time PCR platform. The PRF1 c.900C &gt; T C/T genotype was more frequent among HIV-1 exposed but non-infected children than in HIV-1 positive cases, and the results were confirmed among children infected during breastfeeding. PRF1 c.900C &gt; T correlated with protection against HIV-1 MTCT, suggesting its role in HIV-1 vertical...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: PRF1; Perforin; HIV-1 susceptibility.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400574

A polymorphic microsatellite from the Squalius alburnoides complex (Osteichthyes, Cyprinidae) cloned by serendipity can be useful in genetic analysis of polyploids
Provedor de dados: Genetics and Molecular Biology
Autores: Boto,Luis; Cunha,Carina; Doadrio,Ignacio.
A new microsatellite locus (SAS1) for Squalius alburnoides was obtained through cloning by serendipity. The possible usefulness of this new species-specific microsatellite in genetic studies of this hybrid-species complex, was explored. The polymorphism exhibited by SAS1 microsatellite is an important addition to the set of microsatellites previously used in genetic studies in S. alburnoides complex, that mostly relied in markers described for other species. Moreover, the SAS1 microsatellite could be used to identify the parental genomes of the complex, complementing other methods recently described for the same purpose.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Hybridogenesis; Squalius alburnoides.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300025

Polymorphisms associated with the risk of lung cancer in a healthy Mexican Mestizo population: application of the additive model for cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Pérez-Morales,Rebeca; Méndez-Ramírez,Ignacio; Castro-Hernández,Clementina; Martínez-Ramírez,Ollin C.; Gonsebatt,María Eugenia; Rubio,Julieta.
Lung cancer is the leading cause of cancer mortality in Mexico and worldwide. In the past decade, there has been an increase in the number of lung cancer cases in young people, which suggests an important role for genetic background in the etiology of this disease. In this study, we genetically characterized 16 polymorphisms in 12 low penetrance genes (AhR, CYP1A1, CYP2E1, EPHX1, GSTM1, GSTT1, GSTPI, XRCC1, ERCC2, MGMT, CCND1 and TP53) in 382 healthy Mexican Mestizos as the first step in elucidating the genetic structure of this population and identifying high risk individuals. All of the genotypes analyzed were in Hardy-Weinberg equilibrium, but different degrees of linkage were observed for polymorphisms in the CYP1A1 and EPHX1 genes. The genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Additive model; Genetic polymorphism; Lung cancer; Mexicans; Molecular epidemiology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400003

Homozygous sequence variants in the WNT10B gene underlie split hand/foot malformation
Provedor de dados: Genetics and Molecular Biology
Autores: Ullah,Asmat; Gul,Ajab; Umair,Muhammad; Irfanullah,; Ahmad,Farooq; Aziz,Abdul; Wali,Abdul; Ahmad,Wasim.
Abstract Split-hand/split-foot malformation (SHFM), also known as ectrodactyly is a rare genetic disorder. It is a clinically and genetically heterogeneous group of limb malformations characterized by absence/hypoplasia and/or median cleft of hands and/or feet. To date, seven genes underlying SHFM have been identified. This study described four consanguineous families (A-D) segregating SHFM in an autosomal recessive manner. Linkage in the families was established to chromosome 12p11.1–q13.13 harboring WNT10B gene. Sequence analysis identified a novel homozygous nonsense variant (p.Gln154*) in exon 4 of the WNT10B gene in two families (A and B). In the other two families (C and D), a previously reported variant (c.300_306dupAGGGCGG; p.Leu103Argfs*53) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Split-Hand-Foot Malformation 6; WNT10B gene; Sequence variants.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100001

The karyotype of three Brazilian Terrarana frogs (Amphibia, Anura) with evidence of a new Barycholos species
Provedor de dados: Genetics and Molecular Biology
Autores: Siqueira,Sérgio; Aguiar Jr.,Odair; Pansonato,André; Giaretta,Ariovaldo A.; Strüssmann,Christine; Martins,Itamar; Recco-Pimentel,Shirlei M.
A recent substantial rearrangement of the 882 described eleutherodactyline frog species has considerably improved the understanding of their systematics. Nevertheless, many taxonomic aspects of the South American eleutherodactyline species remain unknown and require further investigation using morphological, cytogenetic and molecular approaches. In this work, the karyotypes of the Brazilian species Ischnocnema juipoca (Atibaia and Campos do Jordão, SP), Barycholos cf. ternetzi (Uberlândia, MG, and Porto Nacional, TO), and Pristimantis crepitans (Chapada dos Guimarães and São Vicente, MT) were analyzed using Giemsa staining, Ag-NOR labeling, and C-banding techniques. All individuals had a diploid number of 22 chromosomes, but the Fundamental Numbers were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Barycholos; Ischnocnema; Pristimantis; Ag-NOR; C-banding.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300008

Molecular analysis of holoprosencephaly in South America
Provedor de dados: Genetics and Molecular Biology
Autores: Savastano,Clarice Pagani; El-Jaick,Kênia Balbi; Costa-Lima,Marcelo Aguiar; Abath,Cristina Maria Batista; Bianca,Sebastiano; Cavalcanti,Denise Pontes; Félix,Têmis Maria; Scarano,Gioacchino; Llerena Jr,Juan Clinton; Vargas,Fernando Regla; Moreira,Miguel Ângelo Martins; Seuánez,Hector N.; Castilla,Eduardo Enrique; Orioli,Iêda Maria.
Holoprosencephaly (HPE) is a spectrum of brain and facial malformations primarily reflecting genetic factors, such as chromosomal abnormalities and gene mutations. Here, we present a clinical and molecular analysis of 195 probands with HPE or microforms; approximately 72% of the patients were derived from the Latin American Collaborative Study of Congenital Malformations (ECLAMC), and 82% of the patients were newborns. Alobar HPE was the predominant brain defect in almost all facial defect categories, except for patients without oral cleft and median or lateral oral clefts. Ethmocephaly, cebocephaly, and premaxillary agenesis were primarily observed among female patients. Premaxillary agenesis occurred in six of the nine diabetic mothers. Recurrence of HPE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Holoprosencephaly; ECLAMC; SHH; ZIC2; SIX3.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200011

Estimates of DNA damage by the comet assay in the direct-developing frog Eleutherodactylus johnstonei (Anura, Eleutherodactylidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Valencia,Laura Carolina; García,Adriana; Ramírez-Pinilla,Martha Patricia; Fuentes,Jorge Luis.
The aim of this study was to use the Comet assay to assess genetic damage in the direct-developing frog Eleutherodactylus johnstonei. A DNA diffusion assay was used to evaluate the effectiveness of alkaline, enzymatic and alkaline/enzymatic treatments for lysing E. johnstonei blood cells and to determine the amount of DNA strand breakage associated with apoptosis and necrosis. Cell sensitivity to the mutagens bleomycin (BLM) and 4-nitroquinoline-1-oxide (4NQO) was also assessed using the Comet assay, as was the assay reproducibility. Alkaline treatment did not lyse the cytoplasmic and nuclear membranes of E. johnstonei blood cells, whereas enzymatic digestion with proteinase K (40 !g/mL) yielded naked nuclei. The contribution of apoptosis and necrosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bleomycin; Comet assay; DNA diffusion assay; Eleutherodactylus johnstonei; 4-nitroquinoline-1-oxide.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400022

Genetic variations in circadian rhythm genes and susceptibility for myocardial infarction
Provedor de dados: Genetics and Molecular Biology
Autores: Škrlec,Ivana; Milic,Jakov; Heffer,Marija; Peterlin,Borut; Wagner,Jasenka.
Abstract Disruption of endogenous circadian rhythms has been shown to increase the risk of developing myocardial infarction (MI), suggesting that circadian genes might play a role in determining disease susceptibility. We conducted a case-control study on 200 patients hospitalized due to MI and 200 healthy controls, investigating the association between MI and single nucleotide polymorphisms (SNPs) in four circadian genes (ARNTL, CLOCK, CRY2, and PER2). The variants of all four genes were chosen based on their previously reported association with cardiovascular risk factors, which have a major influence on the occurrence of myocardial infarction. Statistically significant differences, assessed through Chi-square analysis, were found in genotype...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular diseases; Circadian rhythm; Myocardial infarction; Polymorphisms.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300403

Actin-interacting and flagellar proteins in Leishmania spp.: bioinformatics predictions to functional assignments in phagosome formation
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz,Michely C.; Costa,Marcília P.; Pacheco,Ana C.L.; Kamimura,Michel T.; Silva,Samara C.; Carneiro,Laura D.G.; Sousa,Ana P.L.; Soares,Carlos E.A.; Souza,Celeste S.F.; Oliveira,Diana Magalhães de.
Several motile processes are responsible for the movement of proteins into and within the flagellar membrane, but little is known about the process by which specific proteins (either actin-associated or not) are targeted to protozoan flagellar membranes. Actin is a major cytoskeleton protein, while polymerization and depolymerization of parasite actin and actin-interacting proteins (AIPs) during both processes of motility and host cell entry might be key events for successful infection. For a better understanding the eukaryotic flagellar dynamics, we have surveyed genomes, transcriptomes and proteomes of pathogenic Leishmania spp. to identify pertinent genes/proteins and to build in silico models to properly address their putative roles in trypanosomatid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actin-interacting proteins (AIPs); Flagellar proteins; Leishmania; Coronin and Arp2/3; Phagosome.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300033

Expression profile of genes associated with mastitis in dairy cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Fonseca,Isabela; Silva,Priscila Vendramini; Lange,Carla Christine; Guimarães,Marta F.M.; Weller,Mayara Morena Del Cambre Amaral; Sousa,Katiene Régia Silva; Lopes,Paulo Sávio; Guimarães,José Domingos; Guimarães,Simone E.F.
In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p &lt; 0.05). It was also higher in BW Holstein animals with clinical mastitis (p &lt; 0.001), but it was not significant when Gyr cows with and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokine; Gene expression; Immune response; Real-time PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400018

Clastogenicity of Piper cubeba (Piperaceae) seed extract in an in vivo mammalian cell system
Provedor de dados: Genetics and Molecular Biology
Autores: Junqueira,Adriana Pereira Freire; Perazzo,Fabio Ferreira; Souza,Gustavo Henrique Bianco; Maistro,Edson Luis.
The plant Piper cubeba is widely distributed in tropical and subtropical regions and is used medically for various purposes but has not yet been evaluated for genotoxicity. We used male and female Swiss mice and Wistar rats and the comet assay and micronucleus test to investigate the mutagenic potential of a crude extract of P. cubeba seeds. The rodents were administered 0.5 g kg-1, 1.0 g kg-1 and 1.5 g kg-1 of the extract by gavage. For the Swiss mice, peripheral blood was collected 24 h after treatment for the comet assay, and at 48 and 72 h for the micronucleus test. For the Wistar rats, peripheral blood and hepatic cells were collected for the comet assay and bone marrow cells were collected for the micronucleus test 24 h after treatment. At 1.5 g...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronucleus test; Piper cubeba; Single cell gel electrophoresis (SCGE).
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400025

Growth hormone 1 gene (GH1) polymorphisms as possible markers of the production potential of beef cattle using the Brazilian Canchim breed as a model
Provedor de dados: Genetics and Molecular Biology
Autores: Silveira,Luiz Guilherme G.; Furlan,Luiz R.; Curi,Rogério A.; Ferraz,André Luiz J.; Alencar,Maurício M. de; Regitano,Luciana C.A.; Martins,Cyntia L.; Arrigoni,Mário de Beni; Suguisawa,Liliane; Silveira,Antônio C.; Oliveira,Henrique N. de.
The growth hormone 1 gene (GH1) is a candidate gene for body weight and weight gain in cattle since it plays a fundamental role in growth regulation. We investigated the GH1 gene AluI and DdeI restriction enzyme polymorphisms, located 149 bp apart in the cattle genome, as possible markers of the production potential of Canchim crossbreed cattle, a 5/8 Charolais (Bos taurus) and 3/8 Nelore (Bos indicus) breed developed in Brazil, by evaluating the birth weight, weaning weight, yearling weight and plasma insulin-like growth factor-1 (IGF-1) concentration of 7 month to 10 months old Canchim calves (n = 204) of known genealogy and which had been genotyped for the AluI and DdeI markers. Our results showed significant effect (p &lt; 0.05) between the homozygous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Polymorphism; Growth hormone; Candidate gene.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500012

Absence of mutagenicity in somatic and germ cells of mice submitted to subchronic treatment with an extract of Croton cajucara Benth. (Euphorbiaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Fabio V.; Mesquita,Suzana F.P.; Faria,Maria José S.S.; Poersh,Aline; Maciel,Maria Aparecida M.; Pinto,Angelo C.; Morimoto,Helena K.; Cólus,Ilce Mara S.
The plant Croton cajucara Benth. (Euphorbiaceae) is a medicinal plant from the Brazilian Amazon where it is commonly known as sacaca. The principal compound isolated from C. cajucara stem-bark extracts is the clerodane-type diterpene trans-dehydrocrotonin (DCTN) which presents several biological activities, including antiulcerogenic, anti-inflammatory, hypoglycemic, antimutagenic and antitumoral activity. However, few studies have been carried out to evaluate the therapeutic potential of raw C. cajucara extracts. We studied mutagenicity and antimutagenicity effects of C. cajucara methanol extract using the micronucleus assay in bone marrow cells and the dominant lethal assay in mice submitted to subchronic treatments. The blood testosterone levels of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimutagenicity; Mutagenicity; Dominant lethal; Micronucleus; Croton cajucara; Medicinal plant.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100029

Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes &amp;amp; Jew. ex grassl
Provedor de dados: Genetics and Molecular Biology
Autores: Guimarães,Claudia T.; Honeycutt,Rhonda J.; Sills,Gavin R.; Sobral,Bruno W.S.
Genetic analysis was performed in a population composed of 100 F1 individuals derived from a cross between a cultivated sugarcane (S. officinarum `LA Purple') and its proposed progenitor species (S. robustum `Mol 5829'). Various types (arbitrarily primed-PCR, RFLPs, and AFLPs) of single-dose DNA markers (SDMs) were used to construct genetic linkage maps for both species. The LA Purple map was composed of 341 SDMs, spanning 74 linkage groups and 1,881 cM, while the Mol 5829 map contained 301 SDMs, spanning 65 linkage groups and 1,189 cM. Transmission genetics in these two species showed incomplete polysomy based on the detection of 15% of SDMs linked in repulsion in LA Purple and 13% of these in Mol 5829. Because of this incomplete polysomy, multiple-dose...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100024

Phylogenetic relationships and the evolution of mimicry in the Chauliognathus yellow-black species complex (Coleoptera: Cantharidae) inferred from mitochondrial COI sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Machado,Vilmar; Araujo,Aldo M.; Serrano,José; Galián,José.
The phylogenetic relationships of twelve species of Chauliognathus were investigated by studying the mitochondrial cytochrome oxidase I gene. A 678 bp fragment of the COI gene was sequenced to test the hypothesis that the Müllerian mimicry species of the "yellow-black" complex make up a monophyletic clade, separated from species with other colour patterns. The data set was analysed by neighbour-joining, maximum parsimony and maximum likelihood procedures. The results support a single origin of the yellow-black colour pattern during the evolution of the genus, with one main clade formed by Chauliognathus lineatus, C. tetrapunctatus, C. riograndensis, C. flavipes, C. octomaculatus, C. fallax, and another one formed by two species, C. expansus and C sp 1,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cantharidae; Chauliognathus; COI; Coleoptera; Müllerian mimicry; Phylogeny; Yellow-black complex.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100010

Mitochondrial and chloroplast localization of FtsH-like proteins in sugarcane based on their phylogenetic profile
Provedor de dados: Genetics and Molecular Biology
Autores: Marbach,Phellippe A. Santos; Coelho,Alexandre S. Guedes; Silva-Filho,Marcio C.
A phylogenetic analysis of plant FtsH-like proteins was performed using protein sequences from the GENEBANK database and five groups of plant FtsH-like proteins were identified by neighbor-joining analysis. Prediction of the subcellular location of the proteins suggested that two (FtsH-m1 &amp;amp; FtsH-m2) were mitochondrial and three (FtsH-p1, FtsH-p2, FtsH-p3) were plastid targeting. The phylogenetic profile of plant FtsH-like proteins was used to search sugarcane expressed sequence tag (EST) clusters in the SUCEST database. Initially, 153 clusters presenting homology with FtsH-like proteins were recovered, of which 23 were confirmed by a BLAST search in the GENEBANK database and by comparison of their hidropathy index with that of previously described...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100025

Strand Analysis, a free online program for the computational identification of the best RNA interference (RNAi) targets based on Gibbs free energy
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Tiago Campos; Bittencourt,Vinícius D'Ávila Pascoal; Secolin,Rodrigo; Rocha,Cristiane de Souza; Maia,Ivan de Godoy; Lopes-Cendes,Iscia.
The RNA interference (RNAi) technique is a recent technology that uses double-stranded RNA molecules to promote potent and specific gene silencing. The application of this technique to molecular biology has increased considerably, from gene function identification to disease treatment. However, not all small interfering RNAs (siRNAs) are equally efficient, making target selection an essential procedure. Here we present Strand Analysis (SA), a free online software tool able to identify and classify the best RNAi targets based on Gibbs free energy (deltaG). Furthermore, particular features of the software, such as the free energy landscape and deltaG gradient, may be used to shed light on RNA-induced silencing complex (RISC) activity and RNAi mechanisms,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNAi; SiRNA; SiRNA design; Software.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600030

Effects of caffeine and used coffee grounds on biological features of Aedes aegypti (Diptera, Culicidae) and their possible use in alternative control
Provedor de dados: Genetics and Molecular Biology
Autores: Laranja,Alessandra Theodoro; Manzatto,Antonio José; Campos Bicudo,Hermione Elly Melara de.
Caffeine and used coffee grounds completely blocked the development of Aedes aegypti in the early stages, in treatments with the concentrations 1.0 mg/mL and 50 mg/mL, respectively. More advanced stages and even adults were obtained in lower concentrations of both substances, enabling observations to be made of mortality rate, longevity and esterase patterns. The experiments involved treatments using either eggs or 3rd instar larvae (L3), with or without the addition of fish food. Mortality rates prior to the adult stage and adult longevity were significantly different in the comparisons among treatments, in every kind of experiment, but in those using L3 larvae, their percentages were smaller. Observations of the time of larva and adult onset suggested...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aedes aegypti; Caffeine; Used coffee grounds; Mortality; Longevity; Esterases; Alternative control.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400004

Karyotypic differentiation through chromosome fusion and number reduction in Imparfinis hollandi (Ostariophysi, Heptapteridae)
Provedor de dados: Genetics and Molecular Biology
Autores: Margarido,Vladimir Pavan; Moreira-Filho,Orlando.
The Neotropical Heptapteridae fish Imparfinis hollandi, endemic to the Iguaçu River Basin (Brazil), was cytogenetically analyzed and the diploid chromosome number of 2n = 42 chromosomes was determined (22m + 10sm + 10st), the lowest diploid number in this genus and family. Like other Heptapteridae species, only one NOR-bearing chromosome pair was detected by silver nitrate staining. Dark heterochromatic blocks were visualized in only three chromosome pairs, and chromomycin A3+ bands were coincident with Ag-NORs. Although no intercalary (TTAGGG)n sequence was observed through FISH with a telomere probe, an asymmetric karyotype showing four large chromosome pairs with diploid chromosome number reduction suggests that tandem chromosome fusions probably...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Siluriformes; Chromosome banding; Chromosomal rearrangements; (TTAGGG)n.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200012

Phylogenetic relationships among Brazilian howler monkeys, genus Alouatta (Platyrrhini, Atelidae), based on g1-globin pseudogene sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Meireles,Carla Maria; Czelusniak,John; Ferrari,Stephen F.; Schneider,Maria Paula Cruz; Goodman,Morris.
The genus Alouatta (howler monkeys) is the most widely distributed of New World primates, and has been arranged in three species groups: the Central American Alouatta palliata group and the South American Alouatta seniculus and Alouatta caraya groups. While the latter is monotypic, the A. seniculus group encompasses at least three species (A. seniculus, A. belzebul and A. fusca). In the present study, approximately 600 base pairs of the g1-globin pseudogene were sequenced in the four Brazilian species (A. seniculus, A. belzebul, A. fusca and A. caraya). Maximum parsimony and maximum likelihood methods yielded phylogenetic trees with the same arrangement: {A. caraya [A. seniculus (A. fusca, A. belzebul)]}. The most parsimonious tree had bootstrap values...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300009

Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1
Provedor de dados: Genetics and Molecular Biology
Autores: Du,Yue-feng; Liang,Liang; Shi,Ying; Long,Qing-zhi; Zeng,Jin; Wang,Xin-yang; He,Da-lin.
Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; DNA methylation; DNMT3; RNA interference.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100024

COX-2 gene expression and methylation profile in Sapajus apella as an experimental model for gastric adenocarcinoma
Provedor de dados: Genetics and Molecular Biology
Autores: Rosário Pinheiro,Danilo do; Harada,Maria Lucia; Rodriguez Burbano,Rommel Mario; Nascimento Borges,Barbara do.
Abstract Gastric cancer (GC) remains one of the main causes of cancer-related death worldwide. There are two distinct histological types of GC: diffuse and intestinal. The latter is characterized by the presence of pre-neoplastic lesions. One of the most frequently altered enzymes in intestinal GC is COX-2, an important lesion marker. This work aimed to study COX-2 methylation and expression in N-methyl-N-Nitrosurea (MNU)-induced intestinal GC in six Sapajus apella animals. The partial promoter sequence of S. apella COX-2 gene was obtained and used to identify transcription factors and cis-regulatory element binding sites. The COX-2 methylation pattern was assessed using Methylation-Specific PCR (MSP), and expression was analyzed by immunohistochemistry...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PTGS2; Gene regulation; Animal model; Gastric cancer.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300496

Molecular cloning and in silico analysis of the duck (Anas platyrhynchos) MEF2A gene cDNA and its expression profile in muscle tissues during fetal development
Provedor de dados: Genetics and Molecular Biology
Autores: Liu,Hehe; Wang,Jiwen; Si,Jianmin; Jia,Jing; Li,Liang; Han,Chunchun; Huang,Kailiang; He,Hua; Xu,Feng.
The role of myogenic enhancer transcription factor 2a (MEF2A) in avian muscle during fetal development is unknown. In this work, we cloned the duck MEF2A cDNA sequence (GenBank accession no. HM460752) and examined its developmental expression profiles in cardiac muscle, non-vascular smooth muscle and skeletal muscle. Duck MEF2A cDNA comprised 1479 bp encoding 492 amino acid residues. In silico analysis showed that MEF2A contained MADS (MCM1, AGAMOUS, DEFICIENS and SRF -serum response factor), MEF2 and mitogen-activated protein kinase (MAPK) transcription domains with high homology to related proteins in other species. Modified sites in these domains were conserved among species and several variants were found. Quantitative PCR showed that MEF2A was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Duck MEF2A; Expression profile; In silico analysis; Molecular cloning; Muscle tissues.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100026

Physiological traits of the symbiotic bacterium Teredinibacter turnerae isolated from the mangrove shipworm Neoteredo reynei
Provedor de dados: Genetics and Molecular Biology
Autores: Trindade-Silva,Amaro E.; Machado-Ferreira,Erik; Senra,Marcus V.X.; Vizzoni,Vinicius F.; Yparraguirre,Luciana A.; Leoncini,Orilio; Soares,Carlos A.G.
Nutrition in the Teredinidae family of wood-boring mollusks is sustained by cellulolytic/nitrogen fixing symbiotic bacteria of the Teredinibacter clade. The mangrove Teredinidae Neoteredo reynei is popularly used in the treatment of infectious diseases in the north of Brazil. In the present work, the symbionts of N. reynei, which are strictly confined to the host's gills, were conclusively identified as Teredinibacter turnerae. Symbiont variants obtained in vitro were able to grow using casein as the sole carbon/nitrogen source and under reduced concentrations of NaCl. Furthermore, cellulose consumption in T. turnerae was clearly reduced under low salt concentrations. As a point of interest, we hereby report first hand that T. turnerae in fact exerts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Teredinibacter turnerae; Cellulolytic and nitrogen fixing bacteria; Antibiotic activity; Mangrove shipworm symbiont; Neoteredo reynei.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300022

Engineered scPDL1-DM1 drug conjugate with improved in vitro analysis to target PD-L1 positive cancer cells and intracellular trafficking studies in cancer therapy
Provedor de dados: Genetics and Molecular Biology
Autores: Kalim,Muhammad; Wang,Shenghao; Liang,Keying; Khan,Muhammad Saleem Iqbal; Zhan,Jinbiao.
Abstract Antibody-drug conjugates (ADC), precisely deliver a cytotoxic agent to antigen-expressing tumor cells by using specific binding strategies of antibodies. The ADC has shown the ability of potent bio-therapeutics development but indefinite stoichiometric linkage and full-length antibody penetration compromised the field of its advancement. Single chain variable fragments convention instead of the full-length antibody may overcome the challenge of rapid penetration and internalization. Programmed cell death ligand-1 interaction with PD-1 has recently revolutionized the field of immunotherapy. We systematically designed scPDL1-DM1 drug conjugate by linking scFv-PD-L1 proteins (scFv) with maytansinoids (DM1) cytotoxic agent through succinimidyl...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibody-drug conjugate; Biotherapeutics; Cancer therapy; Intracellular trafficking; PD-L1.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500101

HLA-DRB1 alleles in four Amerindian populations from Argentina and Paraguay
Provedor de dados: Genetics and Molecular Biology
Autores: Parolín,Maria L.; Carnese,Francisco R.
The major histocompatibility complex (MHC) is one of the biological systems of major polymorphisms. The study of HLA class II variability has allowed the identification of several alleles that are characteristic to Amerindian populations, and it is an excellent tool to define the relations and biological affinities among them. In this work, we analyzed the allelic distribution of the HLA-DRB1 class II locus in four Amerindian populations: Mapuche (n = 34) and Tehuelche (n = 23) from the Patagonian region of Argentina, and Wichi SV (n = 24) and Lengua (n = 17) from the Argentinean and Paraguayan Chaco regions, respectively. In all of these groups, relatively high frequencies of Amerindian HLA-DRB1 alleles were observed (DRB1*0403, DRB1*0407, DRB1*0411,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA-DRB1; Polymorphism; Amerindians; Biological affinities.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200002

Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp
Provedor de dados: Genetics and Molecular Biology
Autores: Mo,Fei; Zhao,Jie; Liu,Na; Cao,Li-hua; Jiang,Shan-xiang.
Reference genes are commonly used for normalization of target gene expression during RT-qPCR analysis. However, no housekeeping genes or reference genes have been identified to be stable across different tissue types or under different experimental conditions. To identify the most suitable reference genes for RT-qPCR analysis of target gene expression in the hepatopancreas of crucian carp (Carassius auratus) under various conditions (sex, age, water temperature, and drug treatments), seven reference genes, including beta actin (ACTB), beta-2 microglobulin (B2M), embryonic elongation factor-1 alpha (EEF1A), glyceraldehyde phosphate dehydrogenase (GAPDH), alpha tubulin (TUBA), ribosomal protein l8 (RPL8) and glucose-6-phosphate dehydrogenase (G6PDH), were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; Quantitative RT-PCR; Housekeeping genes; CYP4T; Crucian carp.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400005

[RETRACTION]: Expression of serum microRNA-378 and its clinical significance in renal cell carcinoma
Provedor de dados: Genetics and Molecular Biology
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400950

Spreading the evolutionary synthesis: Theodosius Dobzhansky and genetics in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Araújo,Aldo Mellender de.
The so-called Evolutionary Synthesis, the present paradigm for evolutionary explanations, was established during the 1920s, 1930s and 1940s. One of the leading scientists contributing to this was Theodosius Dobzhansky, a Russian born geneticist who emigrated to the United States of America in 1927 to study with Thomas Hunt Morgan. He was also responsible for the development of Drosophila genetics in Brazil, which was the main organism employed in experimental studies of evolution. Dobzhansky had several opportunities to visit Brazil starting in 1943, to do field and laboratory work as well as teaching. All these activities were fundamental in the spreading of new concepts, methodology, and objectives of the Synthesis to a new audience. This paper discusses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Evolutionary synthesis; Brazilian genetics; Dobzhansky; Drosophila genetics.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300025

A novel 3-base deletion (IVS3+2_4delTGG) of the hydroxymethylbilane synthase gene in a Brazilian patient with acute intermittent porphyria
Provedor de dados: Genetics and Molecular Biology
Autores: Ribeiro,Georgina Severo; Marchiori,Paulo Eurípedes; Hirata,Mário Hiroyuki; Rebecchi,Ivanise; Ozaki,Adriana Natsue; Nagai,Maria Aparecida; Santos,Mariana Lopes dos; Oliveira,Raimundo Antonio Gomes; Barretto,Orlando Cesar de Oliveira.
Acute intermittent porphyria (AIP, OMIM 176000) is an autosomal dominant metabolic disease caused by mutations in the gene encoding hydroxymethylbilane synthase (HMBS; EC 4.3.1.8; formely named porphobilinogen deaminase, PBGD), mapped to chromosome 11q23.3. We describe a novel mutation of the HMBS gene, a de novo 3-base deletion in the splicing donor site of intron 3 (IVS3+2_4delTGG) in a woman affected by AIP. RT-PCR analysis revealed an abnormal HMBS mRNA, compatible with exon 3 skipping.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute intermittent porphyria; Hydroxymethylbilane synthase; Porphobilinogen deaminase; HMBS gene.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600003

Thermal stability of soluble malate dehydrogenase isozymes of subtropical fish belonging to the orders Characiformes, Siluriformes and Perciformes
Provedor de dados: Genetics and Molecular Biology
Autores: Monteiro,Maria do Carmo; Schwantes,Maria Luiza B.; Schwantes,Arno Rudi; Silva,Maria Regina de Aquino.
Electrophoretic thermostability tests of soluble malate dehydrogenases (sMDH) isozymes in tissue extracts of 21 subtropical fish belonging to the orders Characiformes, Siluriformes and Perciformes showed three distinct results. The first, characterized by thermal stability of the slowest-migrating band or A-isoform, was detected in 52% of all species. The second, exhibited in 29% of the species analyzed, had a bidirectionally divergent pattern of their sMDH locus expression, and was characterized by a nondivergent thermostability pattern of both sMDH-A* and B*. In the third category, obtained in 19% of the species studied (the four Siluriformes species), thermostability of the fastest-migrating bands, or B-isoforms, was observed. Comparison of the effects...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200004

Detection of mutator phenotype in Brazilian patients with acute and chronic myeloid leukemia
Provedor de dados: Genetics and Molecular Biology
Autores: Ayres,Flávio Monteiro; Momotuk,Euza Guimarães; Bastos,Celso da Cunha; Cruz,Aparecido Divino da.
The multisteps of tumorigenesis involve the classic chromosomal instability and the mutator phenotype pathways featured by a predisposition to acquire mutations in tumor suppressor genes and oncogenes. Expansion and contraction of microsatellite sequences due to a deficient mismatch repair system are a marker of the mutator phenotype. Controversial results regarding the extent of microsatellite instability (MSI) have been reported in the development and progression of myeloid malignancies. Here, we investigated MSI and loss of heterozygosity (LOH) frequencies at the microsatellite loci BAT-26, D7S486, D8S135, ANK1, IFNA, TP53 and bcr of 19 Brazilian patients with acute (AML) and chronic myeloid leukemia (CML). One AML patient and one CML patient were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loss of heterozygosity; Microsatellite instability; Mismatch repair; Mutator phenotype; Myeloid leukemia.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400003

A methodological approach for the construction of a radiation hybrid map of bovine chromosome 5
Provedor de dados: Genetics and Molecular Biology
Autores: De Donato,M.; Brenneman,R.A.; Stelly,D.M.; Womack,J.E.; Taylor,J.F.
A bovine 5,000 rad WG-RH panel was used to construct an RH map of bovine chromosome 5 (BTA5). Twenty-one microsatellites and thirteen genes were scored in the panel using PAGE and radioactive labeling. Marker retention ranged from 8.9%-25.8% and averaged 17.8%. Pairwise locus analysis placed all markers in a single syntenic group with a LOD support of 4.0. At a LOD support of 8.0, a centromeric group of 23 syntenic markers was formed. Telomeric groups of 11 and 9 markers were assembled with a LOD support of 6.0 and 8.0, respectively. All markers were ordered by maximum likelihood methods using the program RHMAP. Only 13 markers were ordered with a LOD support of at least 3.0, while 25 and 29 markers were ordered with a support of at least 2.0 and 1.0,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RH map; Bovine; Gene mapping.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100005

Genetic divergence among populations and accessions of the spineless peach palm from Pampa Hermosa landrace used in the heart-of-palm agribusiness in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Alves-Pereira,Alessandro; Clement,Charles R.; Picanço-Rodrigues,Doriane.
Although originally domesticated for its fruit, exploitation of the peach palm (Bactris gasipaes Kunth) in the production of gourmet heart-of-palm has also become an important activity, hence the need for improved material for large-scale production, on employing the Pampa Hermosa landrace as the seed source. In this study 11 microsatellite markers were used to evaluate genetic divergence among 96 elite plants representing four populations of spineless peach palm from the above cited source. Genetic variability was high (H T = 0.82). The low levels of divergence [F ST (0.023), G ST' (0.005)] and the high number of migrants (Nm -3.8 to 52.2) indicated significant interpopulation gene flow. Some of the plants presented high levels of genetic divergence, but...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bactris gasipaes; Heart-of-palm; Microsatellites; Genetic divergence; Genetic improvement.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300015

CTLA4 CT60 gene polymorphism is not associated with differential susceptibility to pemphigus foliaceus
Provedor de dados: Genetics and Molecular Biology
Autores: Pincerati,Márcia Regina; Dalla-Costa,Ricardo; Petzl-Erler,Maria Luiza.
Pemphigus foliaceus is an organ-specific autoimmune disease characterized by autoantibodies against the extracellular region of desmoglein 1, a protein that mediates intercellular adhesion in desmosomes. Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) is a key negative regulator of the T cell immune response, playing an important role in T cell homeostasis and maintenance of peripheral tolerance. Polymorphisms in the CTLA4 gene have been associated with autoimmune diseases and the functional CT60 single nucleotide polymorphism (rs3087243, also named 6230G &gt; A) has been proposed to be a casual variant in several of these diseases. The aim of this study was to ascertain whether this polymorphism is associated with inter-individual variation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CTLA4; CT60 polymorphism; Pemphigus; Fogo selvagem; Autoimmunity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300008

Association of T174M polymorphism of angiotensinogen gene with essential hypertension: a meta-analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Liao,Xiaoyang; Yang,Zhiyi; Peng,Daqing; Dai,Hua; Lei,Yi; Zhao,Qian; Han,Yanbing; Wang,Weiwen.
The association between T174M polymorphism of angiotensinogen gene and essential hypertension risk remains controversial. We herein performed a meta-analysis to achieve a reliable estimation of their relationship. All the studies published up to May 2013 on the association between T174M polymorphism and essential hypertension risk were identified by searching the electronic repositories PubMed, MEDLINE and EMBASE, Springer, Elsevier Science Direct, Cochrane Library and Google Scholar. Data were extracted and pooled odds ratios (ORs) with 95% confidence intervals (95% CIs) were calculated. Ultimately, nine eligible studies, including 2188 essential hypertension cases and 2459 controls, were enrolled in this meta-analysis. No significant associations were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essential hypertension; Case-control study; T174M; Polymorphism; Meta-analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400001

Genome composition and pollen viability of Jatropha (Euphorbiaceae) interspecific hybrids by Genomic In Situ Hybridization (GISH)
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Rosilda Cintra de; Marques,Daniela de Argollo; de Carvalho Filho,Marcel Mamede; Oliveira,Ana Rafaela da Silva; Siqueira,Walter José; Benko-Iseppon,Ana Maria; Brasileiro-Vidal,Ana Christina.
Abstract Interspecific hybridization is required for the development of Jatropha curcas L. improved cultivars, due to its narrow genetic basis. The present study aimed to analyze the parental genomic composition of F1 and BC1F1 generations derived from interspecific crosses (J. curcas/J. integerrima and J. curcas/J. multifida) by GISH (Genomic In Situ Hybridization), and the meiotic index and pollen viability of F1 hybrids. In F1 cells from both hybrids, 11 chromosomes of each parental was observed, as expected, but chromosome rearrangement events could be detected using rDNA chromosome markers, suggesting unbalanced cells. In the BC1F1, both hybrids had 22 chromosomes, suggesting that only n = 11 gametes were viable in the next generation. However, GISH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interspecific crosses; Jatropha integerrima; J. multifida; Physic nut; Plant breeding.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500302

Kinases of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae: an overview
Provedor de dados: Genetics and Molecular Biology
Autores: Bailão,Alexandre Melo; Parente,Juliana Alves; Pereira,Maristela; Soares,Célia Maria de Almeida.
Mycoplasma synoviae and Mycoplasma hyopneumoniae are wall-less eubacteria belonging to the class of Mollicutes. These prokaryotes have a reduced genome size and reduced biosynthetic machinery. They cause great losses in animal production. M. synoviae is responsible for an upper respiratory tract disease of chickens and turkeys. M. hyopneumoniae is the causative agent of enzootic pneumonia in pigs. The complete genomes of these organisms showed 17 ORFs encoding kinases in M. synoviae and 15 in each of the M. hyopneumoniae strain. Four kinase genes were restricted to the avian pathogen while three were specific to the pig pathogen when compared to each other. All deduced kinases found in the non pathogenic strain (J[ATCC25934]) were also found in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Kinases; Genomes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200008

Thaptomys Thomas 1915 (Rodentia, Sigmodontinae, Akodontini) with karyotypes 2n = 50, FN = 48, and 2n = 52, FN = 52: two monophyletic lineages recovered by molecular phylogeny
Provedor de dados: Genetics and Molecular Biology
Autores: Ventura,Karen; Silva,Maria José de Jesus; Yonenaga-Yassuda,Yatiyo.
A novel karyotype with 2n = 50, FN = 48, was described for specimens of Thaptomys collected at Una, State of Bahia, Brazil, which are morphologically indistinguishable from Thaptomys nigrita, 2n = 52, FN = 52, found in other localities. It was hence proposed that the 2n = 50 karyotype could belong to a distinct species, cryptic of Thaptomys nigrita, once chromosomal rearrangements observed, along with the geographic distance, might represent a reproductive barrier between both forms. Phylogenetic analyses using maximum parsimony and maximum likelihood based on partial cytochrome b sequences with 1077 bp were performed, attempting to establish the relationships among the individuals with distinct karyotypes along the geographic distribution of the genus;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atlantic Rainforest; Cytochrome b; Endemism; Molecular phylogeny; Thaptomys.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200010

The complete chloroplast genome sequences of three Spondias species reveal close relationship among the species
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Vanessa; Almeida,Cícero.
Abstract This study reports the complete chloroplast sequences of three Spondias species. The genome sequences were obtained for Spondias tuberosa, Spondias bahienses, and Spondias mombin using the Illumina sequencing technology by a combination of de novo methods and a reference-guided assembly using Sapindus mukorossi as reference. The genomes of S. tuberosa, S. bahiensis, and S. mombin had 162,036, 162,218, and 162,302 bp, respectively. The coding regions exhibited 130 genes, including 34–35 tRNAs and 4 rRNAs. The results revealed synteny among the genomes, with high conservation in the gene order and content and CG content. The inverted repeat regions (IRA and IRB) and the large and small single copies were very similar among the three genomes. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plastome; Evolution; Spondias.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100132

Gene amplification in carcinogenesis
Provedor de dados: Genetics and Molecular Biology
Autores: Bizari,Lucimari; Silva,Ana Elizabete; Tajara,Eloiza H.
Gene amplification increases the number of genes in a genome and can give rise to karyotype abnormalities called double minutes (DM) and homogeneously staining regions (HSR), both of which have been widely observed in human tumors but are also known to play a major role during embryonic development due to the fact that they are responsible for the programmed increase of gene expression. The etiology of gene amplification during carcinogenesis is not yet completely understood but can be considered a result of genetic instability. Gene amplification leads to an increase in protein expression and provides a selective advantage during cell growth. Oncogenes such as CCND1, c-MET, c-MYC, ERBB2, EGFR and MDM2 are amplified in human tumors and can be associated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene amplification; Gene expression; Oncogenes; Carcinomas; Double minutes.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100001

YUCCA4 overexpression modulates auxin biosynthesis and transport and influences plant growth and development via crosstalk with abscisic acid in Arabidopsis thaliana
Provedor de dados: Genetics and Molecular Biology
Autores: Munguía-Rodríguez,Aarón Giovanni; López-Bucio,Jesús Salvador; Ruiz-Herrera,León Francisco; Ortiz-Castro,Randy; Guevara-García,Ángel Arturo; Marsch-Martínez,Nayelli; Carreón-Abud,Yazmín; López-Bucio,José; Martínez-Trujillo,Miguel.
Abstract Auxin regulates a plethora of events during plant growth and development, acting in concert with other phytohormones. YUCCA genes encode flavin monooxygenases that function in tryptophan-dependent auxin biosynthesis. To understand the contribution of the YUCCA4 (YUC4) gene on auxin homeostasis, plant growth and interaction with abscisic acid (ABA) signaling, 35S::YUC4 seedlings were generated, which showed elongated hypocotyls with hyponastic leaves and changes in root system architecture that correlate with enhanced auxin responsive gene expression. Differential expression of PIN1, 2, 3 and 7 auxin transporters was detected in roots of YUC4 overexpressing seedlings compared to the wild-type: PIN1 was down-regulated whereas PIN2, PIN3 and PIN7...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arabidopsis; Auxin; Abscisic acid; YUCCA4; Root growth; Germination.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100302

Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair
Provedor de dados: Genetics and Molecular Biology
Autores: Miyaji,E.N.; Johnson,R.T.; Downes,C.S.; Eveno,E.; Mezzina,M.; Sarasin,A.; Menck,C.F.M.
Using a positive selection system for isolating DNA replication and repair related mutants, we isolated a clone from a rat kangaroo cell line (PtK2) that has increased sensitivity to UV light. Characterization of this clone indicated normal post-replication repair after UV irradiation, and normal removal rates of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts by excision repair. However, this cell line has decreased ability to make early incisions on damaged DNA, possibly indicating a defect in preferential repair of actively transcribed genes, and a slower cell proliferation rate, including a longer S-phase. This phenotype reinforces the present notion that control of key mechanisms in cell metabolism, such as cell cycle...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300031

Molecular genotyping of G6PD mutations and Duffy blood group in Afro-descendant communities from Brazilian Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Haiala S. Silva de; Silva,Aylla N. Lima Martins da; Andrade,Gabriela Barreto; Gaia,Karoline Coelho; Costa,Greice de Lemos Cardoso; Santos,Ândrea K. Campos Ribeiro dos; Guerreiro,João Farias.
Abstract Glucose-6-phosphate dehydrogenase deficiency (G6PDd) and Duffy-negative blood group are two red blood cells variants that confer protection against malaria. In this study, the distribution of the most common G6PD variants (G6PD*A-, GGPD*A and G6PD Mediterranean) and the major alleles of the Duffy blood group (FY*A, FY*B and FY*BES) were investigated in an Afro-descendant population from state of Pará, Brazilian Amazon. G6PD variants and Duffy blood group alleles were determined by TaqMan SNP genotyping assay. Overall, molecular genotyping revealed the presence of G6PD variants in 126 (24%) of the individuals studied (5% male and 19% female), and frequencies of the G6PD*A- and G6PD*A alleles were 0.061 and 0.104, respectively. Duffy blood group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: G6PD mutations; Duffy blood group; Afro-descendants; Brazilian Amazon.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500758

Human molecular cytogenetics: from cells to nucleotides
Provedor de dados: Genetics and Molecular Biology
Autores: Riegel,Mariluce.
The field of cytogenetics has focused on studying the number, structure, function and origin of chromosomal abnormalities and the evolution of chromosomes. The development of fluorescent molecules that either directly or via an intermediate molecule bind to DNA has led to the development of fluorescent in situ hybridization (FISH), a technology linking cytogenetics to molecular genetics. This technique has a wide range of applications that increased the dimension of chromosome analysis. The field of cytogenetics is particularly important for medical diagnostics and research as well as for gene ordering and mapping. Furthermore, the increased application of molecular biology techniques, such as array-based technologies, has led to improved resolution,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular cytogenetics; FISH; Array-CGH; Copy number variation; Genomic disorders.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200006

Genetics, evolution and conservation of Bromeliaceae
Provedor de dados: Genetics and Molecular Biology
Autores: Zanella,Camila M.; Janke,Aline; Palma-Silva,Clarisse; Kaltchuk-Santos,Eliane; Pinheiro,Felipe G.; Paggi,Gecele M.; Soares,Luis E.S.; Goetze,Márcia; Büttow,Miriam V.; Bered,Fernanda.
Bromeliaceae is a morphologically distinctive and ecologically diverse family originating in the New World. Three centers of diversity, 58 genera, and about 3,140 bromeliad species are currently recognized. We compiled all of the studies related to the reproductive biology, genetic diversity, and population structure of the Bromeliaceae, and discuss the evolution and conservation of this family. Bromeliads are preferentially pollinated by vertebrates and show marked variation in breeding systems, from predominant inbreeding to predominant outcrossing, as well as constancy in chromosome number (2n = 2x = 50). Autogamous or mixed mating system bromeliads have a high inbreeding coefficient (F IS), while outcrossing species show low F IS. The degree of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromeliads; Cytogenetics; Genetic diversity; Population structure; Reproductive biology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600017

Role of the MAPK pathway in human lung epithelial-like A549 cells apoptosis induced by paraquat
Provedor de dados: Genetics and Molecular Biology
Autores: Sun,Da-Zhuang; Song,Chun-Qing; Xu,Yong-Min; Dong,Xue-Song.
Abstract This study aims to investigate the value of mitogen-activated protein kinases (MAPKs) for paraquat (PQ)-induced apoptosis in human lung epithelial-like A549 cells and the specific mechanism. A549 cell apoptosis were induced by PQ. These cells were divided into six groups: control group (cells were cultured in RPMI-1640 medium); SP600125 group (cells were preconditioned with SP600125); SB203580 group (cells were preconditioned with SB203580); PQ group (cells were treated with PQ); SP600125+PQ group (cells were preconditioned with SP600125 following PQ); SB203580+PQ group (cells were preconditioned with SB203580 following PQ). The cell survival rate, apoptosis rate, and activities of caspase-3 and -9 were detected. When compared with the control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paraquat; A549 cells; MAPKs; Mitochondria; Apoptosis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400701

Clinical and molecular analysis of spinal muscular atrophy in Brazilian patients
Provedor de dados: Genetics and Molecular Biology
Autores: Kim,C.A.; Passos-Bueno,M.R.; Marie,S.K.; Cerqueira,A.; Conti,U.; Marques-Dias,M.J.; Gonzalez,C.H.; Zatz,M.
Spinal muscular atrophy (SMA), the second most common lethal autosomal recessive disorder, has an incidence of 1:10,000 newborns. SMA is divided into acute (Werdnig-Hoffmann disease, type I), intermediate (type II) and juvenile forms (Kugelberg-Welander disease, type III). The gene of all three forms of SMA maps to chromosome 5q 11.2-13.3. Two candidate genes, the survival motor neuron (SMN) gene and the neuronal apoptosis inhibitory protein (NAIP) gene, have been identified; SMN is deleted in most SMA patients. We studied both genes in 87 Brazilian SMA patients (20 type I, 14 type II and 53 type III) from 74 unrelated families, by using PCR and single strand conformation polymorphism (SSCP). Deletions of exons 7 and/or 8 of the SMN gene were found in 69%...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400005

Blepharophimosis, ptosis, epicanthus inversus syndrome (BPES) and cleft lip and palate. Report of two Brazilian families
Provedor de dados: Genetics and Molecular Biology
Autores: Kokitsu-Nakata,N.M.; Richieri-Costa,A.
We have evaluated a girl and a boy with the blepharophimosis, ptosis and epicanthus inversus syndrome (BPES). The girl presented cleft palate and the boy showed cleft lip and palate as additional clinical signs. Both showed familial recurrence in fourth and third generations, respectively. The other family members also presented blepharophimosis, ptosis, and epicanthus inversus, but without lip and palatal involvement. There were no additional clinical signs nor infertility in these patients. To our knowledge this is the first instance of cleft lip and palate reported as additional signs of the BPES syndrome.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200015

Xylella fastidiosa gene expression analysis by DNA microarrays
Provedor de dados: Genetics and Molecular Biology
Autores: Travensolo,Regiane F.; Carareto-Alves,Lucia M.; Costa,Maria V.C.G.; Lopes,Tiago J.S.; Carrilho,Emanuel; Lemos,Eliana G.M.
Xylella fastidiosa genome sequencing has generated valuable data by identifying genes acting either on metabolic pathways or in associated pathogenicity and virulence. Based on available information on these genes, new strategies for studying their expression patterns, such as microarray technology, were employed. A total of 2,600 primer pairs were synthesized and then used to generate fragments using the PCR technique. The arrays were hybridized against cDNAs labeled during reverse transcription reactions and which were obtained from bacteria grown under two different conditions (liquid XDM2 and liquid BCYE). All data were statistically analyzed to verify which genes were differentially expressed. In addition to exploring conditions for X. fastidiosa...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xylella fastidiosa; DNA microarray; Gene expression.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200022

Insulin-like growth factor II gene Apa I polymorphism is not associated with endometriosis susceptibility
Provedor de dados: Genetics and Molecular Biology
Autores: Hsieh,Yao-Yuan; Chang,Chi-Chen; Tsai,Fuu-Jen; Peng,Ching-Tien; Yeh,Lian-Shun; Lin,Cheng-Chieh.
Insulin-like growth factor II (IGF2) has been shown to play a role in abnormal cell growth and carcinogenesis. We investigated if the IGF2 gene Apa I polymorphism at exon 9 was associated with the susceptibility to endometriosis, analyzing 120 women with moderate/severe endometriosis and 103 controls. The genotype frequencies did not differ statistically between the endometriosis (aa = 25.4, ab = 57.4, bb = 17.2%) and control (aa = 20.8 ab = 52.8, bb = 26.4%) groups. The allele frequencies did not differ either: a = 54.1, b = 45.9% among women with endometriosis and a = 47.2, b = 52.8% in the control group. Therefore, no indication was found for an association of this polymorphism with endometriosis susceptibility.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endometriosis; Insulin-like growth factor; IGF-2.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200006

Description of the karyotype of Rhagomys rufescens Thomas, 1886 (Rodentia, Sigmodontinae) from Southern Brazil Atlantic forest
Provedor de dados: Genetics and Molecular Biology
Autores: Testoni,André Filipe; Althoff,Sérgio Luiz; Nascimento,André Paulo; Steiner-Souza,Francisco; Sbalqueiro,Ives José.
Rhagomys rufescens (Rodentia: Sigmodontinae) is an endemic species of the Atlantic forest from Southern and Southeastern Brazil. Some authors consider Rhagomys as part of the tribe Thomasomyini; but its phylogenetic relationships remain unclear. Chromosomal studies on eight specimens of Rhagomys rufescens revealed a diploid number of 2n = 36 and a number of autosome arms FN = 50. GTG, CBG and Ag-NOR banding and CMA3/DAPI staining were performed on metaphase chromosomes. Eight biarmed and nine acrocentric pairs were found in the karyotype of this species. The X and Y chromosomes were both acrocentric. Most of the autosomes and the sex chromosomes showed positive C-bands in the pericentromeric region. The X chromosome showed an additional heterochromatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhagomys rufescens; Thomasomyini; Rodentia; Atlantic forest; Karyotype.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300017

Frequency of the CCRdelta32 allele in Brazilians: a study in colorectal cancer and in HTLV-I infection
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Rinaldo W.; Pires,Edina R.; Duarte,Ana P.M.; Moura,Ricardo P. de; Monteiro,Elisangela; Torloni,Humberto; Proietti,Anna B.; Simpson,Andrew J.G.; Pena,Sérgio D.J.
The identification of a 32-bp deletion in the cc-chemokine receptor-5 gene (CCR5delta32 allele) that renders homozygous individuals highly resistant to HIV infection has prompted worldwide investigations of the frequency of the CCR5delta32 allele in regional populations. It is important to ascertain if CCR5delta32 is a factor to be considered in the overall epidemiology of HIV in individual populations. With this in mind we determined the CCR5delta32 allele frequency in a large sample (907 individuals) of the southeastern Brazilian urban population, stratified as follows: 322 healthy unrelated individuals, 354 unselected colorectal cancer patients, and 229 blood donors. The three groups displayed essentially identical allelic frequencies of CCR5delta32 and...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300003

Genome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs
Provedor de dados: Genetics and Molecular Biology
Autores: Waschburger,Edgar; Kulcheski,Franceli Rodrigues; Veto,Nicole Moreira; Margis,Rogerio; Margis-Pinheiro,Marcia; Turchetto-Zolet,Andreia Carina.
Abstract sn-Glycerol-3-phosphate 1-O-acyltransferase (GPAT) is an important enzyme that catalyzes the transfer of an acyl group from acyl-CoA or acyl-ACP to the sn-1 or sn-2 position of sn-glycerol-3-phosphate (G3P) to generate lysophosphatidic acids (LPAs). The functional studies of GPAT in plants demonstrated its importance in controlling storage and membrane lipid. Identifying genes encoding GPAT in a variety of plant species is crucial to understand their involvement in different metabolic pathways and physiological functions. Here, we performed genome-wide and evolutionary analyses of GPATs in plants. GPAT genes were identified in all algae and plants studied. The phylogenetic analysis showed that these genes group into three main clades. While clades...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant lipids; GPAT enzymes; Phylogeny; Evolution; Gene expression.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200355

Phylogeography and RAPD-PCR variation in Hoplias malabaricus (Bloch, 1794) (Pisces, Teleostei) in southeastern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Dergam,Jorge A.; Paiva,Samuel R.; Schaeffer,Carlos E.; Godinho,Alexandre L.; Vieira,Fabio.
In the Rio Doce basin of southeastern Brazil, the freshwater fish Hoplias malabaricus (trahira) is a widespread predatory characin and one of the few resilient native fishes in a highly impacted lake system. In order to test for genetic differentiation in populations within this basin and for biogeographic relationships among populations of this species in other basins, a study was conducted using RAPD-PCR analysis of Rio Doce samples (N = 63) and phylogeographic analyses with mitochondrial DNA (mtDNA) haplotypes, including the Rio Grande and Macacu river basins. In the Rio Doce basin, the patterns of genetic similarity of RAPD-PCR markers (individual fingerprinting and Nei’s genetic distance) suggest the existence of two genetically different groups, one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RAPD; SSCP; MtDNA; Phylogeography; Hoplias malabaricus; Conservation genetics.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400005

Alpha-1 antitrypsin gene polymorphism in Chronic Obstructive Pulmonary Disease (COPD)
Provedor de dados: Genetics and Molecular Biology
Autores: Denden,Sabri; Khelil,Amel Haj; Knani,Jalel; Lakhdar,Ramzi; Perrin,Pascale; Lefranc,Gérard; Chibani,Jemni Ben.
Alpha-1-antitrypsin (AAT) plays an important role in the pathogenesis of emphysema, the pathological lesion underlying the majority of the manifestations of Chronic Obstructive Pulmonary Disease (COPD). In this study we tested the hypothesis that common AAT polymorphisms influence the risk of developing COPDs. We investigated PiM1 (Ala213Val), PiM2 (Arg101His), PiM3 (Glu376Asp), PiS (Glu264Val) and PiZ (Glu342Lys) SERPINA1 alleles in 100 COPD patients and 200 healthy controls. No significant differences were observed in allele frequencies between COPD patients and controls, neither did haplotype analysis show significant differences between the two groups. A cross-sectional study revealed no significant relationship between common SERPINA1 polymorphisms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-1 antitrypsin; SERPINA1 polymorphisms; COPD; Emphysema; Lung function.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100006

Human aging and somatic point mutations in mtDNA: a comparative study of generational differences (grandparents and grandchildren)
Provedor de dados: Genetics and Molecular Biology
Autores: Marinho,Anderson Nonato do Rosário; Moraes,Milene Raiol de; Santos,Sidney; Santos,Ândrea Ribeiro-dos-.
The accumulation of somatic mutations in mtDNA is correlated with aging. In this work, we sought to identify somatic mutations in the HVS-1 region (D-loop) of mtDNA that might be associated with aging. For this, we compared 31 grandmothers (mean age: 63 ± 2.3 years) and their 62 grandchildren (mean age: 15 ± 4.1 years), the offspring of their daughters. Direct DNA sequencing showed that mutations absent in the grandchildren were detected in a presumably homoplasmic state in three grandmothers and in a heteroplasmic state in an additional 13 grandmothers; no mutations were detected in the remaining 15 grandmothers. However, cloning followed by DNA sequencing in 12 grandmothers confirmed homoplasia in only one of the three mutations previously considered to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; MtDNA; Mitochondrial somatic mutations.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100006

Association between Griffing's diallel and the adaptability and  stability of Eberhart and Russel
Provedor de dados: Genetics and Molecular Biology
Autores: Pacheco,Cleso Antônio Patto; Cruz,Cosme Damião; Santos,Manoel Xavier dos.
The objective of the present work was to provide a methodology to study the inheritance of adaptability and stability through the breakdown of Eberhart and Russell regression coefficients and regression deviations in effects due to the mean and additive genetic effects (gi's and gj's) as well as dominance effects (sij's) of Griffing´s methodology, when the diallel is conducted in several environments. It was concluded that the adaptability and stability parameters are determined in the same manner as are genetic effects. So an F1 cross inherits half the general combining ability (GCA) mean effect from each parent, while the effects due to specific combining ability (SCA) are subjected to the same considerations relative to sij's, i.e., they are dependent...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300027

An investigation into heterogeneity of variance for milk and fat yields of Holstein cows in Brazilian herd environments
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Claudio Napolis.
Heterogeneity of variance in Brazilian herd environments was studied using first-lactation 305-day mature equivalent (ME) milk and fat records of Holstein cows. Herds were divided into two categories, according to low or high herd-year phenotypic standard deviation for ME milk (HYSD). There were 330 sires with daughter records in both HYSD categories. Components of (co)variance, heritability, and genetic correlations for milk and fat yields were estimated using a sire model from bivariate analyses with a restricted maximum likelihood (REML) derivative-free algorithm. Sire and residual variances for milk yield in low HYSD herds were 79 and 57% of those obtained in high HYSD herd. For fat yield they were 67 and 60%, respectively. Heritabilities for milk and...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300015

RAPD markers linked to a block of genes conferring rust resistance to the common bean
Provedor de dados: Genetics and Molecular Biology
Autores: Faleiro,Fábio Gelape; Vinhadelli,Wender Santos; Ragagnin,Vilmar Antonio; Corrêa,Ronan Xavier; Moreira,Maurilio Alves; Barros,Everaldo Gonçalves de.
Rust, caused by the fungus Uromyces appendiculatus, may cause a significant loss to common bean (Phaseolus vulgaris L.) yield. RAPD markers tightly linked to the resistance genes may be used in breeding programs to aid the development of rust-resistant bean cultivars. In this sense, the objective of the present work was to identify RAPD markers linked to a rust resistance gene block present in the cultivar Ouro Negro. Two hundred and fourteen F2 individuals from a cross between the resistant cultivar Ouro Negro and the susceptible cultivar US Pinto 111 were inoculated with a mixture of eight races of U. appendiculatus. The segregation ratio obtained suggested that resistance is monogenic and dominant. Bulked segregant analysis was used in conjunction with...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200027

Genetic diversity and population structure of the Guinea pig (Cavia porcellus, Rodentia, caviidae) in Colombia
Provedor de dados: Genetics and Molecular Biology
Autores: Burgos-Paz,William; Cerón-Muñoz,Mario; Solarte-Portilla,Carlos.
The aim was to establish the genetic diversity and population structure of three guinea pig lines, from seven production zones located in Nariño, southwest Colombia. A total of 384 individuals were genotyped with six microsatellite markers. The measurement of intrapopulation diversity revealed allelic richness ranging from 3.0 to 6.56, and observed heterozygosity (Ho) from 0.33 to 0.60, with a deficit in heterozygous individuals. Although statistically significant (p &lt; 0.05), genetic differentiation between population pairs was found to be low. Genetic distance, as well as clustering of guinea-pig lines and populations, coincided with the historical and geographical distribution of the populations. Likewise, high genetic identity between improved and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food security; Microsatellite; Population structure.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400027

Determination of the antimutagenicity of an aqueous extract of Rhizophora mangle L. (Rhizophoraceae), using in vivo and in vitro test systems
Provedor de dados: Genetics and Molecular Biology
Autores: Malini,Maressa; Marin-Morales,Maria Aparecida; Mantovani,Mário Sérgio; Jamal,Claudia Masrouah; Nati,Natália; Passos,Tatiane da Silva; Matsumoto,Silvia Tamie.
An aqueous extract of Rhizophora mangle L. bark is used as raw material in pottery making in the State of Espirito Santo, Brazil. This extract presents large quantities of tannins, compounds possessing antioxidant properties. Tannin antioxidant activity, as a plant chemical defense mechanism in the process of stabilizing free radicals, has been an incentive to studies on anti-mutagenicity. The present work aimed to evaluate possible antimutagenic activity of a R. mangle aqueous extract, using the Allium cepa test-system and micronuclear (MN) assay with blockage of cytokinesis in Chinese hamster ovary cells (CHO-K1). The Allium cepa test-system indicated antimutagenic activity against the damage induced by the mutagenic agent methyl methanesulfonate. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhizophora mangle; Antimutagenicity; Allium cepa; CHO-K1.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100029

Effects of H2O2, Fe2+ and Fe3+ on curcumin-induced chromosomal aberrations in CHO cells
Provedor de dados: Genetics and Molecular Biology
Autores: Antunes,Lusânia Maria Greggi; Araújo,Maria Cristina Paiva; Dias,Francisca da Luz; Takahashi,Catarina Satie.
The effects of H2O2, Fe2+ and Fe3+ on curcumin-induced clastogenicity were evaluated in CHO cells. Curcumin combined with H2O2 did not increase the chromosomal aberrations more than expected based on a simple additive effect. In contrast, the combination of curcumin-Fe significantly decreased the total number of chromosomal aberrations and the number of abnormal metaphases. The clastogenicity of curcumin may be related to its pro-oxidant properties and its ability to generate free radicals.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CHO; Chromosomal aberrations; Curcumin; Hydrogen peroxide; Mutagenesis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100028

Clonal stability of latex yield in eleven clones of Hevea brasiliensis Muell. Arg.
Provedor de dados: Genetics and Molecular Biology
Autores: Omokhafe,K.O.; Alika,J.E.
Eleven Hevea brasiliensis clones were evaluated for clonal stability of latex yield. A randomized complete block design was used with four replicates, two locations, seven years and three periods per year. Stability analysis was based on clone x year and clone x year x location interactions. Five stability parameters viz environmental variance, shukla's stability variance, regression of clonal latex yield on environmental index, variance due to regression and variance due to deviation from regression were applied. There was significant clone x environment effect at the two levels of interaction. Among the eleven clones, C 162 was outstanding for clonal stability and it can serve as donor parent for stability alleles. Three clones (C 76, C 150 and C 154)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hevea; Clones; Latex; Stability.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300016

Comparative analysis of clustering methods for gene expression time course data
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Ivan G.; Carvalho,Francisco de A. T. de; Souto,Marcílio C. P. de.
This work performs a data driven comparative study of clustering methods used in the analysis of gene expression time courses (or time series). Five clustering methods found in the literature of gene expression analysis are compared: agglomerative hierarchical clustering, CLICK, dynamical clustering, k-means and self-organizing maps. In order to evaluate the methods, a k-fold cross-validation procedure adapted to unsupervised methods is applied. The accuracy of the results is assessed by the comparison of the partitions obtained in these experiments with gene annotation, such as protein function and series classification.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clustering methods; Gene expression time series; Unsupervised cross-validation; Cluster validation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400025

Identification of common bean (Phaseolus vulgaris) duplicates using agromorphological and molecular data
Provedor de dados: Genetics and Molecular Biology
Autores: Chiorato,Alisson Fernando; Carbonell,Sérgio Augusto Morais; Dias,Luiz Antonio dos Santos; Moura,Rafaeli Ramos; Chiavegato,Marilia Barbosa; Colombo,Carlos Augusto.
We used agromorphological and random amplified polymorphic DNA (RAPD) molecular marker data to identify duplicate common bean (Phaseolus vulgaris L. Fabaceae) accessions in the Common Bean Germplasm Bank of the Agronomical Institute - IAC (Banco de Germoplasma de feijoeiro do Instituto Agronômico de Campinas (IAC), SP, Brazil). A total of 116 accessions with the same names and similar agromorphological traits was analyzed. The divergence between the accessions was initially evaluated by means of the agromorphological descriptors using single linkage clustering, from the Euclidean distance. Multivariate analysis identified four duplicate accessions (Carioca Lustroso, Bico de Ouro, Jamapa and Preto), with 17 other same-name accessions being suspected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Multivariate analysis; Germplasm banks; Descriptors.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100020

Variance component estimates applying random regression models for test-day milk yield in Caracu heifers (Bos taurus Artiodactyla, Bovidae)
Provedor de dados: Genetics and Molecular Biology
Autores: El Faro,Lenira; Cardoso,Vera Lucia; Albuquerque,Lucia Galvão de.
Random regression models (RRM) were used to estimate covariance functions for 2,155 first-lactation milk yields of native Brazilian Caracu heifers. The models included contemporary group (defined as year-month of test and paddock) fixed effects, and quadratic effect of age of cow at calving. Genetic and permanent environmental effects were fitted by a random regression model and Legendre polynomials of days in milk (DIM). Schwarz's Bayesian information criteria (BIC) indicated that the best RRM assumed a six coefficient function for both random effects and a sixth order variance function for residual structure. Akaike's information criteria suggested a model with the same number of coefficients for both effects and a residual structure fitted by a step...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Covariance functions; Dairy cattle; Genetic parameters; Longitudinal data; Milk yield.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400011

In silico identification of potential chaperone genes that belong to type III and type IV secretion systems in Xanthomonas axonopodis pv citri
Provedor de dados: Genetics and Molecular Biology
Autores: Khater,Letícia; Santos,Túlio M.; Alegria,Marcos C.; Docena,Cassia; Silva,Ana C.R. da; Ramos,Carlos H.I.
The secretion of bacterial virulence factors and flagellar components requires the assistance of specific type III and flagellar chaperones. Standard computational annotation of the genome of Xanthomonas axonopodis pv citri, a plant pathogen that causes citrus canker, initially did not identify any genes belonging to these chaperone categories since the primary sequence homology between them was very low. However, in a search for hypothetical proteins with characteristics similar to these chaperones, we have now identified 30 chromosomal and 10 plasmidial potential genes encoding chaperones belonging to types III/IV, and flagellar secretion systems in this organism. The significance of these findings is discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chaperone; Protein secretion; Type III and type IV secretion systems; Xanthomonas.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200024

Relative contribution of effects included in contemporary groups for adjusted and actual 120-day and 210-day weights in Nelore cattle in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Pascoa,Lillian; Reyes,Arcadio de los; Elzo,Mauricio A.; Ferreira,Jorge L.; Bezerra,Luiz A.F.; Lôbo,Raysildo B.
The objective of this research was to estimate the relative magnitude of effects included in contemporary groups (CG) and their interactions with adjusted and actual 120 d and 210 d weights in 72, 731 male and female Nelore calves born from 1985 to 2005 in 40 herds from PMGRN (Genetic Improvement Program of Nelore). Ten models with different CG structures were compared. The analyses were done using the general linear models (GLM) procedure run in SAS software. All of the effects included in the CG for each model were significant (p &lt; 0.001) for the four traits analyzed. Inclusion of semester or trimester of birth as part of a CG was more appropriate than its use as an independent effect in the model because it accounted for interactions with the other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Models; Preweaning growth.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400007

Study of the karyotype of Oryzoborus maximiliani (Passeriformes - Aves) using young feather pulp cultures
Provedor de dados: Genetics and Molecular Biology
Autores: Goldschmidt,Beatriz; Nogueira,Denise Monnerat; Silva,Katia Pacheco Araujo; Souza,Lucia Moreno de.
The investigation of the karyotype of Oryzoborus maximiliani in living birds utilizing young feather pulp culture is described. The species karyotype was established as 2n = 72 with unequivocal identification of the sexual chromosome pair. Evolutionary considerations are raised.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200022

A Floricaula/Leafy gene homolog is preferentially expressed in developing female cones of the tropical pine Pinus caribaea var. caribaea
Provedor de dados: Genetics and Molecular Biology
Autores: Dornelas,Marcelo Carnier; Rodriguez,Adriana Pinheiro Martinelli.
In angiosperms, flower formation is controlled by meristem identity genes, one of which, FLORICAULA (FLO)/LEAFY (LFY), plays a central role. It is not known if the formation of reproductive organs of pre-angiosperm species is similarly regulated. Here, we report the cloning of a conifer (Pinus caribaea var. caribaea) FLO/LFY homolog, named PcLFY. This gene has a large C-terminal region of high similarity to angiosperm FLO/LFY orthologs and shorter regions of local similarity. In contrast to angiosperms, conifers have two divergent genes resembling LFY. Gymnosperm FLO/LFY proteins constitute a separate clade, that can be divided into two divergent groups. Phylogenetic analysis of deduced protein sequences has shown that PcLFY belongs to the LFY-like clade....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LEAFY; Plant reproduction; Development; Gene expression; Flowering.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200021

The partial circulant diallel cross at the interpopulation level
Provedor de dados: Genetics and Molecular Biology
Autores: Miranda Filho,José B.; Vencovsky,Roland.
The partial circulant diallel cross mating scheme of Kempthorne and Curnow (Biometrics 17: 229-250, 1961) was adapted for the evaluation of genotypes in crosses at the interpopulation level. Considering a random sample of n lines from base population I, and that each line is crossed with s lines from opposite population II, there will be ns sampled crosses that are evaluated experimentally. The means of the ns sampled crosses and the remaining n(n - s) crosses can be predicted by the reduced model &lt;img src="http:/img/fbpe/gmb/v22n2/0291e1.gif" align="absmiddle"&gt; where Yij is the mean of the cross between line i (i = 1,2,...,n) of population I and line j (j = 1',2',...,n') of population II; µ is the general mean, and gi and gj refer to general...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200020

Taxonomic implications of molecular studies on Northern Brazilian Teredinidae (Mollusca: Bivalvia) specimens
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Sonia Maria Lima; Tagliaro,Claudia Helena; Beasley,Colin Robert; Schneider,Horacio; Sampaio,Iracilda; Santos Filho,Carlos; Müller,Ana Cláudia de Paula.
The current taxonomy of the Teredinidae (shipworms) is wholly based on morphology and up to now no molecular studies of the phylogeny of this group have been published. In the present study the relationships between four genera of the subfamilies Teredininae and Bankiinae were established and the efficiency of the 16S rRNA gene in characterizing four Teredinidae species was tested. Phylogenetic trees support the grouping of Bankia fimbriatula with Nausitora fusticula and of Neoteredo reynei with Psiloteredo healdi, but the genetic distances do not justify the classification of these species into two distinct subfamilies. The results show that B. fimbriatula, N. reynei and P. healdi specimens from the coast of the Brazilian state of Pará have five distinct...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Teredinidae; RRNA16S; Phylogeny; MtDNA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100031

Reproductive strategies and genetic variability in tropical freshwater fish
Provedor de dados: Genetics and Molecular Biology
Autores: Lassala,Maria Dolores Peres; Renesto,Erasmo.
We estimated the genetic variability of nine fish species from the Brazilian upper Paraná River floodplain (Astyanax altiparanae, Hoplias malabaricus, Leporinus lacustris, Loricariichthys platymetopon, Parauchenipterus galeatus, Pimelodus maculatus, Rhaphiodon vulpinus, Roeboides paranensis and Serrasalmus marginatus) based on data for 36 putative allozyme loci obtained using corn starch gel electrophoresis of 13 enzymatic systems: aspartate aminotransferase (EC 2.6.1.1), acid phosphatase (EC 3.1.3.2), esterase (EC 3.1.1.1), glycerol-3-phosphate dehydrogenase (EC 1.1.1.8), glucose-6-phosphate dehydrogenase (EC 1.1.1.49), glucose-6-phosphate isomerase (EC 5.3.1.9), Iditol dehydrogenase (EC 1.1.1.14), isocitrate dehydrogenase - NADP+ (EC 1.1.1.42), L-lactate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Heterozygosity; Paraná River; Polymorphism; Reproductive strategies; Tropical fish.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400030

Over-activation of the Drosophila melanogaster hsp83 gene by selenium intoxication
Provedor de dados: Genetics and Molecular Biology
Autores: Golombieski,Ronaldo Medeiros; Graichen,Daniel Ângelo Sgranzerla; Rocha,João Batista Teixeira da; Valente,Vera Lúcia da Silva; Loreto,Élgion Lúcio da Silva.
Selenium is an important dietary micronutrient and an essential component of selenoproteins and the active sites of some enzymes, although at high concentrations it is toxic. We investigated diphenyl diselenide ((C6H5)2Se2 ) for its effects on the developmental stages of Drosophila melanogaster and found that in the larval and pupae stages the toxic threshold for this compound when added to the banana-agar medium on which the larva were fed was 350 µmol. In adult flies, fed on the same media, there were no observable toxic effects below 500 µmol but there were toxic effects above 600 µmol, indicating that adult flies were more resistant to selenium intoxication. In larvae, a when diphenyl diselenide was present above the toxic threshold there was increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene activation; Hsp83 gene; Selenium; Diphenyl diselenide.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100022

Interactions of polymorphisms in different clock genes associated with circadian phenotypes in humans
Provedor de dados: Genetics and Molecular Biology
Autores: Pedrazzoli,Mario; Secolin,Rodrigo; Esteves,Luiz Otávio Bastos; Pereira,Danyella Silva; Koike,Bruna Del Vechio; Louzada,Fernando Mazzili; Lopes-Cendes,Iscia; Tufik,Sergio.
Several studies have shown that mutations and polymorphisms in clock genes are associated with abnormal circadian parameters in humans and also with more subtle non-pathological phenotypes like chronotypes. However, there have been conflicting results, and none of these studies analyzed the combined effects of more than one clock gene. Up to date, association studies in humans have focused on the analysis of only one clock gene per study. Since these genes encode proteins that physically interact with each other, combinations of polymorphisms in different clock genes could have a synergistic or an inhibitory effect upon circadian phenotypes. In the present study, we analyzed the combined effects of four polymorphisms in four clock genes (Per2, Per3, Clock...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clock genes; Gene interaction; Morningness-eveningness; Sleep; Circadian rhythm.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400005

Karyotypic conservatism in five species of Prochilodus (Characiformes, Prochilodontidae) disclosed by cytogenetic markers
Provedor de dados: Genetics and Molecular Biology
Autores: Voltolin,Tatiana Aparecida; Penitente,Manolo; Mendonça,Bruna Bueno; Senhorini,José Augusto; Foresti,Fausto; Porto-Foresti,Fábio.
The family Prochilodontidae is considered a group with well conserved chromosomes characterized by their number, morphology and banding patterns. Thence, our study aimed at accomplishing a cytogenetic analysis with conventional methods (Giemsa staining, silver staining of the nucleolus organizer regions-AgNOR, and C-banding) and fluorescence in situ hybridization (FISH) with 18S and 5S ribosomal DNA probes in five species of the Prochilodus genus (Prochilodus argenteus, Prochilodus brevis, Prochilodus costatus, Prochilodus lineatus and Prochilodus nigricans) collected from different Brazilian hydrographic basins. The results revealed conservatism in chromosome number, morphology, AgNORs 18S and 5S rDNAs location and constitutive heterochromatin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AgNOR; C-banding; Hydrographic basins; Conserved karyotype; FISH.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300008

Identification, classification and expression pattern analysis of sugarcane cysteine proteinases
Provedor de dados: Genetics and Molecular Biology
Autores: Correa,Gustavo Coelho; Margis-Pinheiro,Márcia; Margis,Rogério.
Cysteine proteases are peptidyl hydrolyses dependent on a cysteine residue at the active center. The physical and chemical properties of cysteine proteases have been extensively characterized, but their precise biological functions have not yet been completely understood, although it is known that they are involved in a number of events such as protein turnover, cancer, germination, programmed cell death and senescence. Protein sequences from different cysteine proteinases, classified as members of the E.C.3.4.22 sub-sub-class, were used to perform a T-BLAST-n search on the Brazilian Sugarcane Expressed Sequence Tags project (SUCEST) data bank. Sequence homology was found with 76 cluster sequences that corresponded to possible cysteine proteinases. The...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100036

Medium-sized deletion in the BRCA1 gene: limitations of Sanger sequencing and MLPA analyses
Provedor de dados: Genetics and Molecular Biology
Autores: Herman,Sandra; Varga,Dominic; Deissler,Heidrun L.; Kreienberg,Rolf; Deissler,Helmut.
We describe a family with a history of breast and ovarian cancer in which MLPA analysis of the BRCA1 gene pointed to a deletion including a part of exon 11. Further characterization confirmed a loss of 374 bp in a region completely covered by conventional sequencing which had not revealed the deletion. Because this alteration was only detected serendipitously with an MLPA probe, we calculated the probabilities of detecting medium-sized deletions in large exons by methods including initial PCR amplification. This showed that a considerable fraction of medium-sized deletions are undetectable by currently used standard methods of mutation analyses. We conclude that long, widely overlapping amplicons should be used to minimize the risk of missing medium-sized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutation analysis; DNA sequencing; PCR; Hereditary breast cancer; Model calculations.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100007

Teratogens: a public health issue – a Brazilian overview
Provedor de dados: Genetics and Molecular Biology
Autores: Mazzu-Nascimento,Thiago; Melo,Débora Gusmão; Morbioli,Giorgio Gianini; Carrilho,Emanuel; Vianna,Fernanda Sales Luiz; Silva,André Anjos da; Schuler-Faccini,Lavinia.
Abstract Congenital anomalies are already the second cause of infant mortality in Brazil, as in many other middle-income countries in Latin America. Birth defects are a result of both genetic and environmental factors, but a multifactorial etiology has been more frequently observed. Here, we address the environmental causes of birth defects – or teratogens – as a public health issue and present their mechanisms of action, categories and their respective maternal-fetal deleterious effects. We also present a survey from 2008 to 2013 of Brazilian cases involving congenital anomalies (annual average of 20,205), fetal deaths (annual average of 1,530), infant hospitalizations (annual average of 82,452), number of deaths of hospitalized infants (annual average of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birth defects; Teratogens; Zika virus; Pregnancy; Public health.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300387

Identification of alternative transcripts of rat CD9 expressed by tumorigenic neural cell lines and in normal tissues
Provedor de dados: Genetics and Molecular Biology
Autores: Wolfahrt,Sonja; Herman,Sandra; Scholz,Claus-Jürgen; Sauer,Georg; Deissler,Helmut.
CD9 is the best-studied member of the tetraspanin family of transmembrane proteins. It is involved in various fundamental cellular processes and its altered expression is a characteristic of malignant cells of different origins. Despite numerous investigations confirming its fundamental role, the heterogeneity of CD9 or other tetraspanin proteins was considered only to be caused by posttranslational modification, rather than alternative splicing. Here we describe the first identification of CD9 transcript variants expressed by cell lines derived from fetal rat brain cells. Variant mRNA-B lacks a potential translation initiation codon in the alternative exon 1 and seems to be characteristic of the tumorigenic BT cell lines. In contrast, variant mRNA-C can...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CD9; Tetraspanin; Transcript; Splice variant; Membrane topology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200019

Exploitation of mitochondrial nad6 as a complementary marker for studying population variability in Lepidoptera
Provedor de dados: Genetics and Molecular Biology
Autores: Silva-Brandão,Karina L.; Lyra,Mariana L.; Santos,Thiago V.; Seraphim,Noemy; Albernaz,Karina C.; Pavinato,Vitor A.C.; Martinelli,Samuel; Cônsoli,Fernando L.; Omoto,Celso.
The applicability of mitochondrial nad6 sequences to studies of DNA and population variability in Lepidoptera was tested in four species of economically important moths and one of wild butterflies. The genetic information so obtained was compared to that of cox1 sequences for two species of Lepidoptera. nad6 primers appropriately amplified all the tested DNA targets, the generated data proving to be as informative and suitable in recovering population structures as that of cox1. The proposal is that, to obtain more robust results, this mitochondrial region can be complementarily used with other molecular sequences in studies of low level phylogeny and population genetics in Lepidoptera.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytochrome c oxidase I; Diatraea saccharalis; DNA polymorphism; Hermeuptychia atalanta; Noctuidae.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400028

Fluorescent amplified fragment length polymorphism (fAFLP) analyses and genetic diversity in Litopenaeus vannamei (Penaeidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Michelle Mantovani; Lemos,Manoel Victor Franco; Galetti Junior,Pedro Manoel; Freitas,Patrícia Domingues de; Furtado Neto,Manuel Antonio Andrade.
The Pacific white shrimp, Litopenaeus vannamei (Penaeidae), represents about 95% of all Brazilian shrimp production. The Brazilian L. vannamei foundation broodstock was made up of specimens collected from different American Pacific sites, but little information was collected on the genetic structure of the broodstock. We used the fluorescence amplified fragment length polymorphism (fAFLP) method to study the genetic diversity of L. vannamei broodstock lines 03CMF1 and 03CBF1 originally produced by breeder-shrimps imported mainly from Panama and Ecuador, although wild individuals from other localities may also have been used in producing these two lines. Our results showed a total of 93 polymorphic bands ranging from 50 to 500 bp, the mean Nei's genetic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: FAFLP; Genetic diversity; Litopenaeus vannamei; Penaeidae; Shrimp.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200016

LNO3 AND L3 Are Associated With Antiproliferative And Pro-Apoptotic Action In Hepatoma Cells
Provedor de dados: Genetics and Molecular Biology
Autores: Zanelatto,Leonardo Campos; Silva,Patrícia Benites Gonçalves da; Sartori,Daniele; Panis,Carolina; Lepri,Sandra; Fátima,Ângelo de; Mantovani,Mário Sérgio.
Abstract The identification of antitumoral substances is the focus of intense biomedical research. Two structural analogues of thalidomide, LNO3 and L3, are two synthetic compounds that might possess such antitumor properties. We evaluated the toxicological effects of these substances, including cytotoxicity, genotoxicity and induction of apoptosis in HTC cells. Additionally, the production of free radicals (nitric oxide and superoxide) was investigated, and the expression of caspases genes 3, 8, and 9 were determined by RT-qPCR. The compounds exhibited cytotoxic effects that resulted in inhibited cell proliferation. LNO3 showed to be more effective and toxic than L3 in all assays. LNO3 stimulated the release of NO and superoxide, which was accompanied by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thalidomide analogues; Antiproliferative activity; Cytotoxicity; Free radicals; Apoptosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200270

Combining ability of elite clones of Eucalyptus grandis and Eucalyptus urophylla with Eucalyptus globulus
Provedor de dados: Genetics and Molecular Biology
Autores: Bison,Odair; Ramalho,Magno Antonio Patto; Rezende,Gabriel Dehon Sampaio Peçanha; Aguiar,Aurélio Mendes; Resende,Marcos Deon Vilela de.
In Brazil, eucalyptus breeding programs for cellulose production has used two species, Eucalyptus grandis and Eucalyptus urophylla. Nevertheless, it would be useful to introgress alleles from other species to improve wood quality and volume. The objective of this research was to evaluate the hybrid potential of elite clones of E. grandis and E. urophylla from the Aracruz Celulose Company S. A. with Eucalyptus globulus clones. To do so, six elite clones were crossed with ten E. globulus clones in a half-diallel mating design. The resulting hybrid combinations as well as the four check clones were evaluated in randomized complete block experiments with single plant plots and 40 replicates from September to October 2001 at three Brazilian sites, Aracruz and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diallel; Eucalyptus breeding; Hybrids; Quantitative genetics; Wood quality.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300019

Identification of novel mutations of Insulin Receptor Substrate 1 (IRS1) in tumor samples of non-small cell lung cancer (NSCLC): Implications for aberrant insulin signaling in development of cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Gorgisen,Gokhan; Hapil,Fatma Zehra; Yilmaz,Ozlem; Cetin,Zafer; Pehlivanoglu,Suray; Ozbudak,Irem Hicran; Erdogan,Abdullah; Ozes,Osman Nidai.
Abstract Lung cancer is the leading cause of cancer-related death, and NSCLC constitutes nearly 85%–90% of all cases. The IRS proteins function as adaptors and transmit signals from multiple receptors. Upon binding of insulin to the insulin receptor (IR), IRS1 is phosphorylated at several YXXM motifs creating docking sites for the binding of PI3Kp85, which activates AKT kinase. Therefore, we thought that gain of function mutantions of IRS1 could be related to development of lung cancer. In line with this, we wanted determine whether the IRS1 gene was mutated in the coding regions surrounding YXXM motifs. We sequenced the coding regions surrounding YXXM motifs of IRS1 using tumor samples of 42 NSCLC patients and 40 matching controls and found heterozygote...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IRS1; NSCLC; Insulin signaling; Lung cancer; IRS proteins.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100015

Non-random distribution of microsatellite motifs and (TTAGGG)n repeats in the monkey frog Pithecopus rusticus (Anura, Phyllomedusidae) karyotype
Provedor de dados: Genetics and Molecular Biology
Autores: Ernetti,Julia R.; Gazolla,Camilla B.; Recco-Pimentel,Shirlei M.; Luca,Elaine M.; Bruschi,Daniel P.
Abstract The monkey frog, Pithecopus rusticus (Anura, Phyllomedusidae) is endemic to the grasslands of the Araucarias Plateau, southern Brazil. This species is known only from a small population found at the type locality. Here, we analyzed for the first time the chromosomal organization of the repetitive sequences, including seven microsatellite repeats and telomeric sequences (TTAGGG)n in the karyotype of the species by Fluorescence in situ Hybridization. The dinucleotide motifs had a pattern of distribution clearly distinct from those of the tri- and tetranucleotides. The dinucleotide motifs are abundant and widely distributed in the chromosomes, located primarily in the subterminal regions. The tri- and tetranucleotides, by contrast, tend to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphibia; Fluorescence in situ Hybridization; Repetitive DNA.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500301

Green turtles (Chelonia mydas) foraging at Arvoredo Island in Southern Brazil: genetic characterization and mixed stock analysis through mtDNA control region haplotypes
Provedor de dados: Genetics and Molecular Biology
Autores: Proietti,Maíra Carneiro; Lara-Ruiz,Paula; Reisser,Júlia Wiener; Pinto,Luciano da Silva; Dellagostin,Odir Antonio; Marins,Luis Fernando.
We analyzed mtDNA control region sequences of green turtles (Chelonia mydas) from Arvoredo Island, a foraging ground in southern Brazil, and identified eight haplotypes. Of these, CM-A8 (64%) and CM-A5 (22%) were dominant, the remainder presenting low frequencies (&lt; 5%). Haplotype (h) and nucleotide ( π) diversities were 0.5570 ± 0.0697 and 0.0021 ± 0.0016, respectively. Exact tests of differentiation and AMOVA ΦST pairwise values between the study area and eight other Atlantic foraging grounds revealed significant differences in most areas, except Ubatuba and Rocas/Noronha, in Brazil (p &gt; 0.05). Mixed Stock Analysis, incorporating eleven Atlantic and one Mediterranean rookery as possible sources of individuals, indicated Ascension and Aves islands...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Foraging grounds; Genetic diversity; Green turtle; MtDNA haplotypes; Natal origins.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300027

Expression analysis of the Arabidopsis thaliana AtSpen2 gene, and its relationship with other plant genes encoding Spen proteins
Provedor de dados: Genetics and Molecular Biology
Autores: Solís-Guzmán,María Gloria; Argüello-Astorga,Gerardo; López-Bucio,José; Ruiz-Herrera,León Francisco; López-Meza,Joel; Sánchez-Calderón,Lenin; Carreón-Abud,Yazmín; Martínez-Trujillo,Miguel.
Abstract Proteins of the Split ends (Spen) family are characterized by an N-terminal domain, with one or more RNA recognition motifs and a SPOC domain. In Arabidopsis thaliana, the Spen protein FPA is involved in the control of flowering time as a component of an autonomous pathway independent of photoperiod. The A. thaliana genome encodes another gene for a putative Spen protein at the locus At4g12640, herein named AtSpen2. Bioinformatics analysis of the AtSPEN2 SPOC domain revealed low sequence similarity with the FPA SPOC domain, which was markedly lower than that found in other Spen proteins from unrelated plant species. To provide experimental information about the function of AtSpen2, A. thaliana plants were transformed with gene constructs of its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spen; Arabidopsis; Vascular bundle; Expression.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400643

Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidis
Provedor de dados: Genetics and Molecular Biology
Autores: Chiang,Hsin-I; Berghman,Luc R.; Zhou,Huaijun.
The NF-kB pathway plays an important role in regulating the immunity response in animals. In this study, small interfering RNAs (siRNA) were used to specifically inhibit NF-kB 1 expression and to elucidate the role of NF-kB in the signal transduction pathway of the Salmonella challenge in the chicken HD11 cell line. The cells were transfected with either NF-kB 1 siRNA, glyceraldehyde 3-phosphate dehydrogenase siRNA (positive control) or the negative control siRNA for 24 h, followed by Salmonella enteritidis (SE) challenge or non-challenge for 1 h and 4 h. Eight candidate genes related to the signal pathway of SE challenge were selected to examine the effect of NF-kB 1 inhibition on their expressions by mRNA quantification. The results showed that, with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA interference; NF-kBp50; Macrophage; Salmonella; Chicken.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300013

Genetic signatures of parental contribution in black and white populations   in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Guerreiro-Junior,Vanderlei; Bisso-Machado,Rafael; Marrero,Andrea; Hünemeier,Tábita; Salzano,Francisco M.; Bortolini,Maria Cátira.
Two hundred and three individuals classified as white were tested for 11 single nucleotide polymorphisms plus two insertion/deletions in their Y-chromosomes. A subset of these individuals (n = 172) was also screened for sequences in the first hypervariable segment of their mitochondrial DNA (mtDNA). In addition, complementary studies were done for 11 of the 13 markers indicated above in 54 of 107 black subjects previously investigated in this southern Brazilian population. The prevalence of Y-chromosome haplogroups among whites was similar to that found in the Azores (Portugal) or Spain, but not to that of other European countries. About half of the European or African mtDNA haplogroups of these individuals were related to their places of origin, but not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Admixture dynamics; MtDNA; Y-chromosome markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100001

Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris
Provedor de dados: Genetics and Molecular Biology
Autores: Teh,Ser Huy; Fong,Mun Yik; Mohamed,Zulqarnain.
The Pichia pastoris expression system was used to produce recombinant human erythropoietin, a protein synthesized by the adult kidney and responsible for the regulation of red blood cell production. The entire recombinant human erythropoietin (rhEPO) gene was constructed using the Splicing by Overlap Extension by PCR (SOE-PCR) technique, cloned and expressed through the secretory pathway of the Pichia expression system. Recombinant erythropoietin was successfully expressed in P. pastoris. The estimated molecular mass of the expressed protein ranged from 32 kDa to 75 kDa, with the variation in size being attributed to the presence of rhEPO glycosylation analogs. A crude functional analysis of the soluble proteins showed that all of the forms were active in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erythropoietin; Glycosylation; Pichia pastoris; SOE-PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300017

Diversity within American cassava germ plasm based on RAPD markers
Provedor de dados: Genetics and Molecular Biology
Autores: Colombo,Carlos; Second,Gérard; Charrier,André.
This work focuses on the genetic diversity of American cassava through RAPD molecular markers. The 126 genotypes studied were distributed on four geographical levels ranging from local to continental. Samples included ethnocultivars from the Santa Isabel community in the Brazilian Amazon, local cultivars collected in the State of São Paulo, native accessions from very diverse Brazilian regions, and representative accessions from the Centro Internacional de Agricultura Tropical (CIAT) core collection. Eighty-eight polymorphic bands were analyzed. Results revealed the weak genetic structure of the cassava analyzed. The pattern formed by the first two axes of the principal coordinates analysis (PCoA) revealed an overlapping of the São Paulo State genotype,...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100034

A comparison of the hygienic response of Africanized and European (Apis mellifera carnica) honey bees to Varroa-infested brood in tropical Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Aumeier,Pia; Rosenkranz,Peter; Gonçalves,Lionel Segui.
In order to examine the significance of hygienic behavior for the tolerance to varroosis of Africanized honey bees, they were compared with non-tolerant Carniolans in tropical Brazil. Capped worker brood cells were artificially infested with living Varroa mites, and inspected some days later. Uncapping, disappearance of the introduced mite and removal of the pupa were recorded in a total of manipulated 3,096 cells during three summer seasons. The hygienic response varied between Africanized and Carniolan colonies, but this difference was significant only in one year, during which Africanized honey bees removed a significantly greater proportion of Varroa mites than European honey bees. A high proportion of the mites disappeared from artificially infested...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400013

Regulation of Na+ and K+ homeostasis in plants: towards improved salt stress tolerance in crop plants
Provedor de dados: Genetics and Molecular Biology
Autores: Almeida,Diego M.; Oliveira,M. Margarida; Saibo,Nelson J. M.
Abstract Soil salinity is a major abiotic stress that results in considerable crop yield losses worldwide. However, some plant genotypes show a high tolerance to soil salinity, as they manage to maintain a high K+/Na+ ratio in the cytosol, in contrast to salt stress susceptible genotypes. Although, different plant genotypes show different salt tolerance mechanisms, they all rely on the regulation and function of K+ and Na+ transporters and H+ pumps, which generate the driving force for K+ and Na+ transport. In this review we will introduce salt stress responses in plants and summarize the current knowledge about the most important ion transporters that facilitate intra- and intercellular K+ and Na+ homeostasis in these organisms. We will describe and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salinity; Sodium; Potassium; Proton pumps; Ion transporters.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200326

Evaluation of MC1R high-throughput nucleotide sequencing data generated by the 1000 Genomes Project
Provedor de dados: Genetics and Molecular Biology
Autores: Marano,Leonardo Arduino; Marcorin,Letícia; Castelli,Erick da Cruz; Mendes-Junior,Celso Teixeira.
Abstract The advent of next-generation sequencing allows simultaneous processing of several genomic regions/individuals, increasing the availability and accuracy of whole-genome data. However, these new approaches may present some errors and bias due to alignment, genotype calling, and imputation methods. Despite these flaws, data obtained by next-generation sequencing can be valuable for population and evolutionary studies of specific genes, such as genes related to how pigmentation evolved among populations, one of the main topics in human evolutionary biology. Melanocortin-1 receptor (MC1R) is one of the most studied genes involved in pigmentation variation. As MC1R has already been suggested to affect melanogenesis and increase risk of developing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melanocortin-1 Receptor; The 1000 Genomes Project; Next-generation sequencing.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300530

Description and comparison of growth parameters in Chianina and Nelore cattle breeds
Provedor de dados: Genetics and Molecular Biology
Autores: Carrijo,Sônia Mara; Duarte,Francisco A. Moura.
Weight data from birth to 18 months of age of Nelore and Chianina, both meat-producing cattle breeds, were analyzed. Data were corrected for significant effects of environment and utilized to estimate genetic parameters through the non-linear von Bertalanffy model. Average values found for growth parameters in Nelore were: mature weight (A), 312.87 kg; integration constant (B), 0.49; maturity rate (k), 0.13; age at inflection point (T(I)), 3.29 months; weight at inflection point (P(I)), 92.70 kg, and maturity interval (1/k), 8.04 months. For the Chianina animals, the values were 751.38 kg, 0.59, 0.10, 6.64 months, 222.63 kg, and 10.98 months, respectively. Nelore animals exhibited higher maturity rate, smaller maturity intervals, reaching mature weights...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200009

Development of a rapid phenotypic test for HCV protease inhibitors with potential use in clinical decisions
Provedor de dados: Genetics and Molecular Biology
Autores: Pessoa,Luciana Santos; Vidal,Luãnna Liebscher; Costa,Emmerson C.B. da; Abreu,Celina Monteiro; Cunha,Rodrigo Delvecchio da; Valadão,Ana Luiza Chaves; Santos,André Felipe dos; Tanuri,Amilcar.
Abstract Approximately 185 million people worldwide are chronically infected with hepatitis C virus (HCV). The first-wave of approved NS3 protease inhibitors (PIs) were Telaprevir and Boceprevir, which are currently discontinued. Simeprevir is a second-wave PI incorporated into the Brazilian hepatitis C treatment protocol. Drug resistance plays a key role in patients' treatment regimen. Here, we developed a simple phenotypic assay to evaluate the impact of resistance mutations in HCV NS3 protease to PIs, using a protein expression vector containing wild type NS3 protease domain and NS4A co-factor. We analyzed the impact of five resistance mutations (T54A, V36M, V158I, V170I and T54S+V170I) against Telaprevir, Boceprevir and Simeprevir. Protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protease inhibitor; Phenotypic test; Hepatitis C virus; HCV NS3 protease; Resistance mutation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300358

C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Maffei,Eliane Mariza Dortas; Pompolo,Silvia das Graças; Petitpierre,Eduard.
The aim of this study was to describe mitotic and meiotic chromosomes of Cycloneda sanguinea using C-banding, fluorescent in situ hybridization (FISH) rDNA probes, and sequential FISH/Ag-NOR staining. The chromosome number was 2n = 18 + XX for females and 2n = 18 + Xy for males. The X chromosome was metacentric and the Y chromosome was very small. During meiosis, the karyotypic meioformula was n = 9 + Xy p, and sex chromosomes configured a parachute at metaphase I. At the beginning of pachytene, bivalents were still individualized, and sex chromosomes were associated end-to-end through the heteropycnotic region of the X chromosome. Later in pachytene, further condensation led to the formation of a pseudo-ring by the sex bivalent. All chromosomes showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In situ hybridization; RDNA sequences; Sequential FISH/Ag-NOR staining; C-banding; Cycloneda sanguinea.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200011

Genetic distances and taxonomic trees between goats of Ceará State (Brazil) and goats of the Mediterranean region (Europe and Africa)
Provedor de dados: Genetics and Molecular Biology
Autores: Machado,Théa M.M.; Chakir,Mohamed; Lauvergne,Jean Jacques.
Goats of an undefined breed (called UDB) from the State of Ceará, northeastern Brazil (N = 447), European Mediterranean goats (N = 3,847) and African Mediterranean goats (N = 325) were compared to establish genetic distances and taxonomic trees. Allelic frequencies in each population for presence or absence of the following traits were used: horns, reduced ears, long hair, wattles, beard, roan color, brown eumelanin and eumelanic standard pigmentation. The genetic distance, applying the method developed by Nei (1972), was: least between goats from different meso-regions of the State of Ceará (0.0008 to 0.0120); small between all UDB of Ceará and French goats of Rove and Haute Roya (0.0236 and 0.0459); greater between all UDB of Ceará and northern Spanish...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100022

Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids
Provedor de dados: Genetics and Molecular Biology
Autores: Hagel,Jillian M.; Krizevski,Raz; Kilpatrick,Korey; Sitrit,Yaron; Marsolais,Frédéric; Lewinsohn,Efraim; Facchini,Peter J.
Khat (Catha edulis Forsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenylpropylamino alkaloids (1S,2S)-cathine and (1R,2S)-norephedrine is not well characterized in plants. We prepared a cDNA library from young khat leaves and sequenced 4,896 random clones, generating an expressed sequence tag (EST) library of 3,293 unigenes. Putative functions were assigned to &gt; 98% of the ESTs, providing a key resource for gene discovery. Candidates potentially involved at various stages of phenylpropylamino alkaloid biosynthesis from L-phenylalanine to (1S,2S)-cathine were identified.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Khat; (S)-cathinone; Phenylpropylamino alkaloids biosynthesis; EST library; Gene discovery.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400017

Identification of 2nd chromosome region translocated onto the W chromosome by RFLP with EST-cDNA clones in the Gensei-kouken strains of the mulberry silkworm, Bombyx mori L
Provedor de dados: Genetics and Molecular Biology
Autores: Sreekumar,Sivaramakurup; Kadono-Okuda,Keiko; Nagayasu,Ken-ichi; Hara,Wajiro.
In silkworms, sex-limited strains are either obtained spontaneously or induced by X-rays or gamma rays. When a fragment of an autosome carrying a dominant allele of those genes responsible for certain characters is translocated onto a W chromosome, the female of the successive generations will express these phenotypic characters and sex discrimination can be facilitated. Gensei-kouken strains are sex-limited strains of silkworms developed by irradiating the pupae with gamma rays, by which a portion of the second chromosome is translocated onto the W chromosome. In these improved strains, the females are yellow-blooded and spin yellow cocoons. By using the EST-cDNA clones mapped on the Z chromosome, we identified the sex according to the polymorphic banding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sex-limited strains; Chromosomal translocation; CDNA markers; Restricted fragment length polymorphism; Mulberry silkworm.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100007

Sunkifolias and Buxisunkis: Sexually obtained reciprocal hybrids of Citrus sunki x Severinia buxifolia
Provedor de dados: Genetics and Molecular Biology
Autores: Medina-Filho,Herculano P.; Bordignon,Rita; Ballvé,Rosa M.L.
The present investigation reports reciprocal hybrids between Citrus sunki, belonging to the "True Citrus" taxonomic group, and Severinia buxifolia of the "Primitive Citrus" group. This represents the first case of sexual hybridization between genera of distinct taxonomic groups of the subtribe Citrinae, which have several complementary agronomic and phytosanitary attributes. Thus, there exists considerable interest in determining the potential of such hybrids as rootstocks, as well as a possible genetic bridge to combine, by breeding at diploid level, nuclear and cytoplasmic genes of Severinia with Citrus and other citrus intergeneric hybrids.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100021

Chromosomal polymorphism in Drosophila willistoni populations from Uruguay
Provedor de dados: Genetics and Molecular Biology
Autores: Valente,Vera Lúcia da Silva; Goñi,Beatriz; Valiati,Victor Hugo; Rohde,Cláudia; Morales,Nena Basílio.
Chromosomal polymorphism in natural populations of Drosophila willistoni from Uruguay and southern Brazil was investigated in order to understand the genetic characteristics and evolutionary potential of these almost geographically marginal populations. The level of chromosomal polymorphism in samples from Uruguay was higher than in those from the southernmost Brazilian state of Rio Grande do Sul. The increase in the polymorphism of these populations, in which the species almost reaches its southern limit, contradicts the low level of paracentric inversion polymorphism expected under the central-marginal chromosomal polymorphism cline previously reported. The high frequency of some inversions and the presence of unique inversions in samples from Uruguay...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila willistoni; Chromosome inversion polymorphism; Uruguayan natural populations.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200009

Interferon lambda 4 (IFNL4) gene polymorphism is associated with spontaneous clearance of HCV in HIV-1 positive patients
Provedor de dados: Genetics and Molecular Biology
Autores: Alves,Camila Fernanda da Silveira; Grott,Camila Schultz; Lunge,Vagner Ricardo; Béria,Jorge Umberto; Tietzmann,Daniela Cardoso; Stein,Airton Tetelbom; Simon,Daniel.
Abstract Approximately one-third of the individuals infected with human immunodeficiency virus type 1 (HIV-1) are co-infected with hepatitis C virus (HCV). Co-infected patients have an increased risk for developing end-stage liver diseases. Variants upstream of the IFNL3 gene have been associated with spontaneous and treatment-induced clearance of HCV infection. Recently, a novel polymorphism was discovered, denoted IFNL4 ΔG &gt; TT (rs368234815), which seems to be a better predictor of spontaneous clearance than the IFNL4 rs12979860 polymorphism. We aimed to determine the prevalence of the IFNL4 ΔG &gt; TT variants and to evaluate the association with spontaneous clearance of HCV infection in Brazilian HIV-1 patients. The IFNL4 ΔG &gt; TT genotypes were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: IFNL4 genotypes; HIV/HCV co-infected patients; Spontaneous clearance.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300374

Comparative cytogenetic analysis between Lonchorhina aurita and Trachops cirrhosus (Chiroptera, Phyllostomidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Barros,Helen Maria Duarte do Rêgo; Sotero-Caio,Cibele Gomes de; Santos,Neide; Souza,Maria José de.
Phyllostomidae comprises the most diverse family of neotropical bats, its wide range of morphological features leading to uncertainty regarding phylogenetic relationships. Seeing that cytogenetics is one of the fields capable of providing support for currently adopted classifications through the use of several markers, a comparative analysis between two Phyllostomidae species was undertaken in the present study, with a view to supplying datasets for the further establishment of Phyllostomidae evolutionary relationships. Karyotypes of Lonchorhina aurita (2n = 32; FN = 60) and Trachops cirrhosus (2n = 30; FN = 56) were analyzed by G- and C-banding, silver nitrate staining (Ag-NOR) and base-specific fluorochromes. Chromosomal data obtained for both species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bats; Chromosome banding; Fluorochromes; NOR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400014

Sugarcane transcriptome: A landmark in plant genomics in the tropics
Provedor de dados: Genetics and Molecular Biology
Autores: Arruda,Paulo.
Tipo: Info:eu-repo/semantics/other
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100001

Phylogenetic relationships and karyotype evolution in the sigmodontine rodent Akodon (2n = 10 and 2n = 16) from Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Maria José de J.; Patton,James L.; Yonenaga-Yassuda,Yatiyo.
Comparison of G-bands from 2n = 10 and 2n = 16 karyotypes of Akodon revealed tandem fusions, pericentric inversions and Robertsonian rearrangement in autosomes and addition/deletion of constitutive heterochromatin in sex chromosomes. Cytochrome-b sequences indicate that the 2n = 10 karyotype is a new species and show it to be a sister taxon of the 2n = 14, 2n = 15 and 2n = 16 karyotypes. Indeed, this group shows a particular evolutionary situation in which a unique taxonomic unit based on morphological data can be detected, but, karyologically, it can be separated into two groups (2n = 14-15-16 and 2n = 10). Cytochrome-b sequences show a finer resolution, indicating that these four karyotypes represent three molecular entities (2n = 14-15, 2n = 16 and 2n =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Akodon; Cytochrome-b; G-bands; Karyotypical evolution; Phylogeny.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300012

Tocantins river as an effective barrier to gene flow in Saguinus niger populations
Provedor de dados: Genetics and Molecular Biology
Autores: Vallinoto,Marcelo; Araripe,Juliana; Rego,Péricles S. do; Tagliaro,Claudia H.; Sampaio,Iracilda; Schneider,Horacio.
The Saguinus represent the basal genus of the Callitrichinae subfamily. Traditionally this genus is divided into three groups: Hairy, Mottled and Bare-face, however, molecular data failed to validate these groups as monophyletic units, as well as raised some subspecies to the species status. This is the case of the former subspecies Saguinus midas midas and S. midas niger, which are now considered as different species. In the present study, we sequenced a portion of the D-loop mtDNA region in populations from the East bank of the Xingu and from both banks of the Tocantins river, in order to test the effectiveness of large rivers as barriers to the gene flow in Saguinus. According to our results, the populations from the East and West banks of the Tocantins...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Primates; Saguinus niger; D-loop; MtDNA; Tocantins river.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200005

Identification of significant pathways in gastric cancer based on protein-protein interaction networks and cluster analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Hu,Kongwang; Chen,Feihu.
Gastric cancer is one of the most common and lethal cancers worldwide. However, despite its clinical importance, the regulatory mechanisms involved in the aggressiveness of this cancer are still poorly understood. A better understanding of the biology, genetics and molecular mechanisms of gastric cancer would be useful in developing novel targeted approaches for treating this disease. In this study we used protein-protein interaction networks and cluster analysis to comprehensively investigate the cellular pathways involved in gastric cancer. A primary immunodeficiency pathway, focal adhesion, ECM-receptor interactions and the metabolism of xenobiotics by cytochrome P450 were identified as four important pathways associated with the progression of gastric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Graph clustering; Pathway crosstalk; Protein-protein interaction network.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400023

Cytogenetics of the neotropical flesh fly Pattonela intermutans (Diptera, Sarcophagidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Parise-Maltempi,Patricia Pasquali; Avancini,Rita Maria Pereira.
Pattonella intermutans has 2n = 12 chromosomes including three metacentric and two submetacentric pairs of autosomes and an XX/XY sex chromosome pair. The autosomes are characterized by the presence of a C band in the pericentromeric region while sex chromosomes are totally heterochromatic. The FISH technique showed a nucleolar organizer region (NOR) in autosome IV.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300011

Additional value of a combined genetic risk score to standard cardiovascular stratification
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Andreia; Mendonca,Maria Isabel; Borges,Sofia; Sousa,Ana Célia; Freitas,Sónia; Henriques,Eva; Rodrigues,Mariana; Freitas,Ana Isabel; Guerra,Graça; Freitas,Carolina; Pereira,Décio; Brehm,António; Reis,Roberto Palma Dos.
Abstract The utility of genetic risk scores (GRS) as independent risk predictors remains inconclusive. Here, we evaluate the additive value of a multi-locus GRS to the Framingham risk score (FRS) in coronary artery disease (CAD) risk prediction. A total of 2888 individuals (1566 coronary patients and 1322 controls) were divided into three subgroups according to FRS. Multiplicative GRS was determined for 32 genetic variants associated to CAD. Logistic Regression and Area Under the Curve (AUC) were determined first, using the TRF for each FRS subgroup, and secondly, adding GRS. Different models (TRF, TRF+GRS) were used to classify the subjects into risk categories for the FRS 10-year predicted risk. The improvement offered by GRS was expressed as Net...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary artery disease; Genetic risk score; Framingham score; Risk prediction; Risk factors.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500766

Sequence analysis of three citrus viroids infecting a single Tunisian citrus tree (Citrus, reticulata, Clementine)
Provedor de dados: Genetics and Molecular Biology
Autores: Elleuch,Amine; Khouaja,Fattouma Djilani; Hamdi,Imen; Bsais,Nabiha; Perreault,Jean-Pierre; Marrakchi,Mohamed; Fakhfakh,Hatem.
We report the nucleotide sequences of three citrus viroids belonging to three different genera: Citrus exocortis viroid (CEVd), Hop stunt viroid (HSVd) and Citrus viroid-III (CVd-III) isolated from a single natural infected Citrus reticulata var. Clementine tree growing in a tree nursery in Manouba (near Tunis Capital). We describe the sequence variability of these viroids from their natural host without using an alternative passage by an indicator host or an artificial inoculation. This work confirms that naturally occurring viroid infections contain a mixture of sequence variants. These are the first sequences of citrus viroids from Africa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CEVd; CVd-III; Citrus; HSVd; Quasi-species; Sequence variability; Viroid.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400020

Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines
Provedor de dados: Genetics and Molecular Biology
Autores: Garcia,Antonio A. F.; Benchimol,Luciana L.; Barbosa,Antônia M. M.; Geraldi,Isaias O.; Souza Jr.,Cláudio L.; Souza,Anete P. de.
In order to compare their relative efficiencies as markers and to find the most suitable marker for maize diversity studies we evaluated 18 inbred tropical maize lines using a number of different loci as markers. The loci used were: 774 amplified fragment length polymorphisms (AFLPs); 262 random amplified polymorphic DNAs (RAPDs); 185 restriction fragment length polymorphisms (RFLPs); and 68 simple sequence repeats (SSR). For estimating genetic distance the AFLP and RFLP markers gave the most correlated results, with a correlation coefficient of r = 0.87. Bootstrap analysis were used to evaluate the number of loci for the markers and the coefficients of variation (CV) revealed a skewed distribution. The dominant markers (AFLP and RAPD) had small CV values...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diversity studies; Genetic distances; Maize; Molecular markers.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400019

The karyotype of Adenomera diptyx (Boettger 1885) (Anura, Leptodactylidae) from northeastern Argentina
Provedor de dados: Genetics and Molecular Biology
Autores: Zaracho,Víctor Hugo; Hernando,Alejandra Beatriz.
In this work we analyzed the karyotype of five populations of Adenomera diptyx from Argentina after conventional staining, Ag-NOR and C-banding. All specimens presented 2n = 26 and FN = 34. The karyotype was formed by three submetacentric, one metacentric and nine telocentric pairs. Silver staining revealed that the NOR was located on a secondary constriction in pair 7. C- banding evidenced constitutive heterochromatin at the pericentromeric region of all chromosomes. The karyotype of A. diptyx was similar to that of A. hylaedactyla (2n = 26, FN = 34) and different from that of A. andreae (2n = 26, FN = 40) in the fundamental number and secondary constriction position. It also differed from the karyotypes of A. marmorata (2n = 24, FN = 34 and 36) and of A....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adenomera diptyx; Argentina; Standard karyotype; Ag-NOR; C-bands.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100016

Isoenzymatic polymorphism in Citrus spp. and Poncirus trifoliata (L.) Raf. (Rutaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Novelli,Valdenice Moreira; Machado,Marcos Antonio; Lopes,Catalina Romero.
Isoenzymatic polymorphism analysis was used to determine genetic variability among species and hybrids of Citrus spp. and one accession of Poncirus trifoliata (L.) Raf. Ten enzymatic systems aspartate aminotransferase (AAT), acid phosphatase (ACP), leucine aminopeptidase (LAP), 6-phosphogluconate dehydrogenase (6-PGD), isocitrate dehydrogenase (IDH), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), diaphorase (DIA), shikimate dehydrogenase (SKD) and peroxidase (PRX) were analyzed. Twenty loci and 48 alleles were identified. Sweet orange cultivars (C. sinensis (L). Osbeck) showed the highest polymorphism with the largest number of heterozygous loci, although the alleles of those loci were the same in all cultivars, with the exception of Westin and...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100030

Holobionts and their hologenomes: Evolution with mixed modes of inheritance
Provedor de dados: Genetics and Molecular Biology
Autores: Haag,Karen Luisa.
Abstract Symbioses are ubiquitous and have played an influential role in the evolution of life on Earth. Genomic studies are now revealing a huge diversity of associations among hosts and their microbiotas, allowing us to characterize their complex ecological and evolutionary dynamics. The different transmission modes and the asynchronous cell proliferation of the numerous symbionts associated with one host generate a genomic conflict ought to be solved. Two disputing views have been used to model and predict the outcome of such conflicts. The traditional view is based on community ecology, and considers that selection at the level of individuals is sufficient to explain longstanding associations among species. A new perspective considers that the host and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genomics; Metagenomics; Microbiota; Symbiosis; Holobiont.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200189

Metformin (dimethyl-biguanide) induced DNA damage in mammalian cells
Provedor de dados: Genetics and Molecular Biology
Autores: Amador,Rubem R.; Longo,João Paulo Figueiró; Lacava,Zulmira G.; Dórea,José G.; Santos,Maria de Fátima M. Almeida.
Metformin (dimethyl-biguanide) is an insulin-sensitizing agent that lowers fasting plasma-insulin concentration, wherefore it's wide use for patients with a variety of insulin-resistant and prediabetic states, including impaired glucose tolerance. During pregnancy it is a further resource for reducing first-trimester pregnancy loss in women with the polycystic ovary syndrome. We tested metformin genotoxicity in cells of Chinese hamster ovary, CHO-K1 (chromosome aberrations; comet assays) and in mice (micronucleus assays). Concentrations of 114.4 µg/mL and 572 µg/mL were used in in vitro tests, and 95.4 mg/kg, 190.8 mg/kg and 333.9 mg/kg in assaying. Although the in vitro tests revealed no chromosome aberrations in metaphase cells, DNA damage was detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metformin; DNA damage; Chromosome aberration.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100022

Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas
Provedor de dados: Genetics and Molecular Biology
Autores: Veiga,Luciana CS; Bérgamo,Nádia A; Kowalski,Luiz Paulo; Rogatto,Silvia R.
Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FISH; Chromosomal aberrations; Head and neck cancer; Chromosome 17.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200003

Structural characterization of the bglH gene encoding a beta-glucosidase-like enzyme in an endophytic Bacillus pumilus strain
Provedor de dados: Genetics and Molecular Biology
Autores: Bogas,Andréa C.; Watanabe,Maria Angelica E.; Barbosa,Aneli; Vilas-Boas,Laurival A.; Bonatto,Ana C.; Dekker,Robert; Souza,Emanuel M.; Fungaro,Maria Helena P.
A beta-glucosidase-like enzyme-encoding gene (bglH) of an endophytic Bacillus pumilus strain (CL16) was cloned using a shotgun genomic library constructed in Escherichia coli. The nucleotide sequence of the entire cloned fragment (2484 bp) was determined and characterized. An incomplete open reading frame (ORF) of 534 bp (ORF1) designated bglP and a complete ORF of 1419 bp (ORF2) designated bglH, located in the fragment, are organized in an operon. The protein deduced from 1419 bp (ORF2) had 472 amino acid residues without a characteristic signal peptide sequence, suggesting that the enzyme is localized in the cytoplasm. The amino acid sequence deduced from bglH gene had high similarity with beta-glucosidases from the glycosyl hydrolase family 1....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus pumilus; BglH; Glucosidase; Glycosyl hydrolase 1; PTS.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100018

SSD - a free software for designing multimeric mono-, bi- and trivalent shRNAs
Provedor de dados: Genetics and Molecular Biology
Autores: Carli,Gabriel José de; Rotela,Abdon Troche; Lubini,Greice; Contiliani,Danyel Fernandes; Candia,Nidia Benítez; Depintor,Thiago S.; Abreu,Fabiano Carlos Pinto de; Simões,Zilá Luz Paulino; Ríos,Danilo Fernández; Pereira,Tiago Campos.
Abstract RNA interference (RNAi) is a powerful gene silencing technology, widely used in analyses of reverse genetics, development of therapeutic strategies and generation of biotechnological products. Here we present a free software tool for the rational design of RNAi effectors, named siRNA and shRNA designer (SSD). SSD incorporates our previously developed software Strand Analysis to construct template DNAs amenable for the large scale production of mono-, bi- and trivalent multimeric shRNAs, via in vitro rolling circle transcription. We tested SSD by creating a trivalent multimeric shRNA against the vitellogenin gene of Apis mellifera. RT-qPCR analysis revealed that our molecule promoted a decrease in more than 50% of the target mRNA, in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene silencing; SiRNA; Multimeric; ShRNA; Free software.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100802

R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Maistro,Edson Luis; Foresti,Fausto; Oliveira,Claudio.
The absence of longitudinal bands in fish chromosomes has been associated with technical problems in chromosome preparations or the absence of a structural compartmentalization in the fish genome. In the present study, a R-banding pattern was obtained using a replication banding technique by in vivo treatment with 5-bromodeoxyuridine (5-BrdU). G-banding patterns were obtained after trypsin treatment and also after chromosome cleavage by in situ treatment with the restriction endonuclease BamHI. A similar G-banding pattern was also obtained after cleavage with the endonuclease HinfI. Presence of a resolute R- and G-banding patterns shows that Astyanax scabripinnis paranae chromosomes could present an isochore-like structure similar to that found in other...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200011

Decreased serum PON1 arylesterase activity in familial hypercholesterolemia patients with a mutated LDLR gene
Provedor de dados: Genetics and Molecular Biology
Autores: Idrees,Muhammad; Siddiqi,Abdul Rauf; Ajmal,Muhammad; Akram,Muhammad; Khalid,Rana Rehan; Hussain,Alamdar; Qamar,Raheel; Bokhari,Habib.
Abstract Paraoxonase 1 (PON1) is a serum enzyme associated with high density lipoprotein (HDL) regulation through its paraoxonase and arylesterase activity. PON1 inhibits the oxidation of HDL and low density lipoprotein (LDL), and is involved in the pathogenesis of a variety of diseases including atherosclerosis. Conversely, mutations in the low density lipoprotein receptor (LDLR) result in failure of receptor mediated endocytosis of LDL leading to its elevated plasma levels and onset of familial hypercholesterolemia (FH). In the current study we investigated the role of PON1 polymorphisms rs662; c.575A &gt; G (p.Gln192Arg) and rs854560; c.163T &gt; A (p.Leu55Met) in a large family having FH patients harboring a functional mutation in LDLR. Genotypes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paraoxonase-1; Hypercholesteremia; Arylesterase; LDLR mutation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400570

Screening for suppressors of temperature sensitivity in a yeast mutant defective in vacuolar protein degradation
Provedor de dados: Genetics and Molecular Biology
Autores: Götte,Martin.
Vps33p is a member of the Sec1/munc18-like protein (SM protein) family involved in vesicular protein transport to the yeast vacuole. It is part of a high molecular weight complex which is required for homotypic vacuole fusion, and participates in Golgi-to-endosome and endosome-to-vacuole transport steps. Deletions in the vps33 gene result in severe vacuolar protein sorting and vacuolar morphology defects. We used a temperature sensitive (ts) vps33 deletion strain in a high copy plasmid suppressor approach to identify genes possibly acting along the same vesicular trafficking pathway(s) as Vps33p. While only the original VPS33 gene could restore the vacuolar enzyme sorting and vacuolar morphology defects, several suppressors of temperature sensitivity were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: VPS33; UFD4; Lysosomal trafficking; Ubiquitin; Endosome.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100015

Response to recurrent selection under small effective population size
Provedor de dados: Genetics and Molecular Biology
Autores: Souza Jr.,Cláudio Lopes de; Geraldi,Isaias Olívio; Vencovsky,Roland.
A formula was derived for the prediction of the response to recurrent selection when the effective population size (Ne) is small. Usually, responses to selection have been estimated by Rs = icsigma²A/sigmaPh, where i, c, sigma²A, and sigmaPh stand for standardized selection differential, parental control, additive variance, and phenotypic standard deviation, respectively. This expression, however, was derived under the assumption of infinite population size. By introducing the effects of finite population size, the expression derived was Rs = [ic(sigma²A + deltaFD1)/sigmaPh] - DFID, where deltaF, ID and D1 are the changes in the inbreeding coefficient, the inbreeding depression, and the covariance of additive and homozygous dominance effects, respectively....
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400023

A genetic framework for flowering-time pathways in Citrus spp.
Provedor de dados: Genetics and Molecular Biology
Autores: Dornelas,Marcelo Carnier; Camargo,Raquel Luciana Boscariol; Figueiredo,Luciana Harumi Morimoto; Takita,Marco Aurélio.
Floral transition is one the most drastic changes occurring during the life cycle of a plant. The shoot apical meristem switches from the production of leaves with associated secondary shoot meristems to the production of flower meristems. This transition is abrupt and generally irreversible, suggesting it is regulated by a robust gene regulatory network capable of driving sharp transitions. The moment at which this transition occurs is precisely determined by environmental and endogenous signals. A large number of genes acting within these pathways have been cloned in model herbaceous plants such as Arabidopsis thaliana. In this paper, we report the results of our search in the Citrus expressed sequence tag (CitEST) database for expressed sequence tags...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reproductive development; Flowering-time genes; Reproduction; Photoperiod; CONSTANS.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500006

Functional properties of the three hemoglobin phenotypes of Nelore cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Di Vito,A.; Schwantes,A.R.; Schwantes,M.L.B.
Blood and stripped hemoglobin from Nelore cattle individuals were submitted to oxygen equilibrium experiments in both gasometric and spectrophotometric methods. No difference was detected in oxygen affinity and Bohr effect among Hb A, Hb B and Hb AB in experiments with both blood and stripped hemoglobin, in the presence and absence of the chloride ion. However, n values (Hill plots) were higher in Hb B and Hb AB (&gt;2) than in Hb A (&lt;2).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allohemoglobins; Functional properties; Nelore cattle; Cooperativity; Bohr effect; Modulatators effect.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200004

Epistasis of polymorphisms related to the articular cartilage extracellular matrix in knee osteoarthritis: Analysis-based multifactor dimensionality reduction
Provedor de dados: Genetics and Molecular Biology
Autores: Fernández-Torres,Javier; Martínez-Nava,Gabriela Angélica; Zamudio-Cuevas,Yessica; Lozada,Carlos; Garrido-Rodríguez,Daniela; Martínez-Flores,Karina.
Abstract Osteoarthritis (OA) is a complex disease with a multifactorial etiology. The genetic component is one of the main associated factors, resulting from interactions between genes and environmental factors. The aim of this study was to identify gene-gene interactions (epistasis) of the articular cartilage extracellular matrix (ECM) in knee OA. Ninety-two knee OA patients and 147 healthy individuals were included. Participants were genotyped in order to evaluate nine variants of eight genes associated with ECM metabolism using the OpenArray technology. Epistasis was analyzed using the multifactor dimensionality reduction (MDR) method. The MDR analysis showed significant gene-gene interactions between MMP3 (rs679620) and COL3A1 (rs1800255), and between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epistasis; Extracellular matrix; Knee osteoarthritis; Multifactor dimensionality reduction; Polymorphisms.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400102

Mating and offspring frequencies under partial outcrossing in a structured population
Provedor de dados: Genetics and Molecular Biology
Autores: Stark,Alan E.
This paper gives a model of a structured population with respect to an autosomal locus with two alleles. The population reproduces in discrete and non-overlapping generations. The population is assumed to be in equilibrium in that exactly the same distribution of genotypic proportions is reproduced in each generation. The population is subdivided into 'localities' which are characterized by the local gene frequencies. Within each locality the genotypic proportions may depart from Hardy-Weinberg proportions and the same fixation index applies to all localities. The system departs from reality by assuming that the frequency of the first allele follows the beta distribution. However, this enables a convenient way to derive the mating frequencies of parents so...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Subdivided populations; F-statistics; Mating frequencies.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100003

Robertsonian rearrangements in the reef fish Chromis (Perciformes, Pomacentridae) involving chromosomes bearing 5s rRNA genes
Provedor de dados: Genetics and Molecular Biology
Autores: Molina,Wagner F.; Galetti-Jr,Pedro M.
Cytogenetic studies were done on three Pomacentridae species of the genus Chromis. The karyotype of C. multilineata consisted of 48 acrocentric chromosomes (FN = 48), C. insolata had 2n = 46-47 (3-4M+6SM+36-38A; FN = 56) and C. flavicauda had 2n = 39 (9M+6SM+24A; FN = 54). Robertsonian polymorphisms were detected in C. insolata and C. flavicauda. All three species had small heterochromatic blocks restricted to centromeric regions. Nucleolar organizer regions (NORs) were detected in the telomeric position of a medium acrocentric chromosome pair in C. multilineata and in non-homologous chromosomes in both C. flavicauda and C. insolata. FISH with a telomeric probe detected no internal telomeric sequences in C. flavicauda and C. insolata. 5S rRNA genes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromis; FISH; Polymorphism; Robertsonian rearrangements; 5S rDNA; Telomeric sequences.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400004

Secondary structure of nrDNA Internal Transcribed Spacers as a useful tool to align highly divergent species in phylogenetic studies
Provedor de dados: Genetics and Molecular Biology
Autores: Giudicelli,Giovanna C.; Mäder,Geraldo; Silva-Arias,Gustavo A.; Zamberlan,Priscilla M.; Bonatto,Sandro L.; Freitas,Loreta B.
Abstract Recently, it has been suggested that internal transcribed spacer (ITS) sequences are under selective constraints to preserve their secondary structure. Here, we investigate the patterns of the ITS nucleotide and secondary structure conservation across the Passiflora L. genus to evaluate the potential use of secondary structure data as a helpful tool for the alignment in taxonomically complex genera. Considering the frequent use of ITS, this study also presents a perspective on future analyses in other plant groups. The ITS1 and ITS2 sequences presented significant differences for mean values of the lowest energy state (LES) and for number of hairpins in different Passiflora subgenera. Statistical analyses for the subgenera separately support...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora; Lowest energy state; Hairpins; ITS1; ITS2.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200191

(TTTA)n polymorphism of CYP19 (aromatase gene) in Euro- and Afro-Brazilians
Provedor de dados: Genetics and Molecular Biology
Autores: Kvitko,Kátia; Nunes,Janice Corrêa de Barros; Hutz,Mara Helena.
We investigated the polymorphic tetranucleotide repeat (TTTA)n located in the fourth intron of the CYP19 gene in two Brazilian populations. The frequencies of the five common alleles (A) in Euro- and Afro-Brazilians were, respectively: seven repeats (A5), 0.586 and 0.80; eight repeats (A4), 0.092 and 0.06; nine repeats (A3), 0.014 and 0.01; eleven repeats (A2), 0.284 and 0.09; twelve repeats (A1), 0.021 and 0.04. In addition, one Euro-Brazilian individual had a rare allele with 13 repeats. The allelic frequencies in Euro- and Afro-Brazilians differed statistically (p &lt; 10-3). The two samples were found to be in Hardy-Weinberg equilibrium (p = 0,828 and p = 0,995).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CYP19; Gene polymorphism.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300005

Analysis of variance of partial diallel tables
Provedor de dados: Genetics and Molecular Biology
Autores: Viana,José Marcelo Soriano; Cruz,Cosme Damião; Cardoso,Antonio Américo; Regazzi,Adair José.
The theory of variance analysis of partial diallel tables, following Hayman's proposal of 1954, is presented. As several statistical tests yield similar inferences, the present analysis mainly proposes to assess genetic variability in two groups of parents and to study specific, varietal and mean heteroses. Testing the nullity of specific heteroses equals testing absence of dominance. Testing equality of varietal heteroses of the parents of a group is equivalent to testing the hypothesis that in the other group allelic genes have the same frequency. Rejection of the hypothesis that the mean heterosis is null indicates dominance. The information obtained complements that provided by diallel analysis involving parents and their F1 hybrids or F2 generations....
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100040

Associations of MYF5 gene polymorphisms with meat quality traits in different domestic pig (Sus scrofa) populations
Provedor de dados: Genetics and Molecular Biology
Autores: Liu,Min; Peng,Jian; Xu,Dequan; Zheng,Rong; Li,Feng’e; Li,Jialian; Zuo,Bo; Lei,Minggang; Xiong,Yuanzhu; Deng,Changyan; Jiang,Siwen.
The MYF5 gene is first inducibly expressed in muscle cell during embryonic muscle development and plays an important role in regulating the differentiation of skeletal muscle precursors. In this study we used PCR-RFLP to investigate two pig (Sus scrofa) populations (n = 302) for two MYF5 gene polymorphisms, a previously unreported novel Met-Leu shift single nucleotide polymorphism (SNP) MYF5/Hsp92II located on exon 1 and the previously identified intron 1 MYF5/HinfI SNP. Haplotype and association analysis showed that haplotypes of the two SNPs were significantly associated with drip loss rate (DLR, p &lt; 0.05), water holding capacity (WHC, p &lt; 0.05), biceps femoris meat color value (MCV2, p &lt; 0.05), biceps femoris marbling score (MM2, p &lt; 0.01),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meat quality; MYF5 gene; Pigs; Polymorphism; Sus scrofa.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300012

Cytogenetic characterization of the strongly electric Amazonian eel, Electrophorus electricus (Teleostei, Gymnotiformes), from the Brazilian rivers Amazon and Araguaia
Provedor de dados: Genetics and Molecular Biology
Autores: Fonteles,Soraia B.A.; Lopes,Carlos E.; Akama,Alberto; Fernandes,Flora M.C.; Porto-Foresti,Fábio; Senhorini,José A.; Daniel-Silva,Maria de Fátima Z.; Foresti,Fausto; Almeida-Toledo,Lurdes Foresti de.
A karyotype analysis of the electric eel, Electrophorus electricus (Teleostei, Gymnotiformes), a strongly electric fish from northern South America, is presented. Two female specimens were analyzed, one from the Amazon River and one from the Araguaia River. The specimens had a chromosomal number of 2n = 52 (42M-SM + 10A). C-bands were present in a centromeric and pericentromeric position on part of the chromosomes; some interstitial C-bands were also present. Heteromorphic nucleolus organizer regions (NORs) were detected in two chromosome pairs of the specimen from the Amazon River. The chromosome number and karyotype characteristics are similar to those of other Gymnotidae species. The genera Electrophorus and Gymnotus are positioned as the basal lineages...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome; Fish; Gymnotidae; Electric eel.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200010

Diallel analysis of fruit set in Hevea brasiliensis Muell. Arg.
Provedor de dados: Genetics and Molecular Biology
Autores: Omokhafe,Kenneth O.; Akpobome,Frederick A.; Nasiru,Ibrahim.
The clonal and inter-clonal combining ability of fruit set in a Hevea brasiliensis four-parent diallel mating was evaluated using a randomized complete block experimental design with three replicates. Twelve main and reciprocal crosses were hand pollinated and percentage fruit set was recorded. The raw data were subjected to an arc-sine transformation for analysis of variance, and general and specific combining ability. There was significant variation of each of fruit set for the various crosses, general combining ability and reciprocal effect. The breeding implications of these results are also discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fruit set; Hevea; Combining ability.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300021

Overall picture of expressed Heat Shock Factors in Glycine max, Lotus japonicus and Medicago truncatula
Provedor de dados: Genetics and Molecular Biology
Autores: Soares-Cavalcanti,Nina M.; Belarmino,Luís C; Kido,Ederson A.; Pandolfi,Valesca; Marcelino-Guimarães,Francismar C.; Rodrigues,Fabiana A.; Pereira,Gonçalo A.G.; Benko-Iseppon,Ana M.
Heat shock (HS) leads to the activation of molecular mechanisms, known as HS-response, that prevent damage and enhance survival under stress. Plants have a flexible and specialized network of Heat Shock Factors (HSFs), which are transcription factors that induce the expression of heat shock proteins. The present work aimed to identify and characterize the Glycine max HSF repertory in the Soybean Genome Project (GENOSOJA platform), comparing them with other legumes (Medicago truncatula and Lotus japonicus) in view of current knowledge of Arabidopsis thaliana. The HSF characterization in leguminous plants led to the identification of 25, 19 and 21 candidate ESTs in soybean, Lotus and Medicago, respectively. A search in the SuperSAGE libraries revealed 68...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HSF; Fabaceae; Bioinformatics; Abiotic stress; Transcription factor.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200006

Genetic variability of two populations of Pseudoplatystoma reticulatum from the Upper Paraguay River Basin
Provedor de dados: Genetics and Molecular Biology
Autores: Abreu,Marcia Matos de; Pereira,Luiz Henrique Garcia; Vila,Vilma Barretto; Foresti,Fausto; Oliveira,Claudio.
Catfishes of the genus Pseudoplatystoma are very important species due to both their high commercial value and their ecological role as voracious predators. They undertake lengthy migratory movements during their life-cycle, this including reproductive migration which occurs from October to December in the rainy season. In the present study, seven microsatellite loci were analyzed to access genetic variability in two samples of P. reticulatum from the Upper Paraguay Basin. The loci were highly polymorphic (mean = 7.28). According to all analysis, the two samples of P. reticulatum revealed pronounced genetic differentiation. Fst value was 0.2290, Rst value 0.1067 and AMOVA 22.90% (Fst) and 10.67% (Rst), all being highly significant (p &lt; 0.001). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Population genetics; Microsatellite; Homing; Population structure.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400032

Characterization of Brazilian soybean cultivars using microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Priolli,Regina Helena Geribello; Mendes-Junior,Celso Teixeira; Arantes,Neylson Eustáquio; Contel,Eucleia Primo Betioli.
Microsatellite markers or SSR (Simple Sequence Repeats) have proved to be an excellent tool for cultivar identification, pedigree analysis and the evaluation of genetic distance among organisms. Soybean cultivars have been characterized mainly by morphological and biochemical traits. However, these traits have not been sufficient to characterize the large number of cultivars eligible to receive protection under the Brazilian Cultivar Protection Act. In order to define new soybean cultivar markers, the alleles of twelve SSR loci of 186 Brazilian soybean cultivars were studied by estimating the variation in their size range and their respective frequencies. On average, 5.3 alleles per locus were detected, with a mean genetic diversity of 0.64 ± 0.12. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max (L.) Merrill; Simple sequence repeat; Microsatellites; Molecular markers; Soybean elite cultivars.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200012

Genetic polymorphisms at the leptin receptor gene in three beef cattle breeds
Provedor de dados: Genetics and Molecular Biology
Autores: Almeida,Sabrina E.M.; Santos,Luciana B.S.; Passos,Daniel T.; Corbellini,Ângela O.; Lopes,Beatriz M.T.; Kirst,Cláudia; Terra,Gustavo; Neves,Jairo P.; Gonçalves,Paulo B.D.; Moraes,José C.F.; Weimer,Tania de Azevedo.
The genetic diversity of a single nucleotide polymorphism (SNP) at the exon 20 (T945M) of the leptin receptor gene (LEPR) and of three short tandem repeats (STRs BM7225, BMS694, and BMS2145) linked to LEPR was investigated in three beef cattle herds (Brangus Ibagé, Charolais, and Aberdeen Angus). A cheap and effective new method to analyze the T945M polymorphism in cattle populations was developed and the possible role of these polymorphisms in reproduction and weight gain of postpartum cows was evaluated. High levels of genetic diversity were observed with the average heterozygosity of STRs ranging from 0.71 to 0.81. No significant association was detected between LEPR markers and reproductive parameters or daily weight gain. These negative results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Genetic diversity; Cattle production; LEPR gene polymorphisms.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400013

Soybean seed oil content: genetic  control under different photoperiods
Provedor de dados: Genetics and Molecular Biology
Autores: Miranda,Zilda F.S.; Arias,Carlos A. Arrabal; Toledo,José Francisco Ferraz de; Oliveira,Marcelo Fernandes de.
The oil content of soybean (Glycine max (L.) Merrill) seeds is a polygenic and complex trait that is responsive to environmental effects that occur during plant development. Our objective was to study the seed oil content of soybeans developed under diverse photoperiod and temperature conditions. Three parental inbred lines with classic (BR-13, FT-2 and BR85-29009) and one with long juvenile flowering type (OCEPAR 8) and the F2, F3, and F9 generations derived from all possible crosses between them (including reciprocals) were sowed in September 27th, October 20th and December 17th in 1993 in Londrina, Paraná State, Brazil (between 23o08'47" and 23o55'46" latitude S). The October and December sowing dates are within the period the varietal research...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300017

Isolation and characterization of CaMF3, an anther-specific gene in Capsicum annuum L.
Provedor de dados: Genetics and Molecular Biology
Autores: Hao,Xuefeng; Chen,Changming; Chen,Guoju; Cao,Bihao; Chen,Qinghua; Lei,Jianjun.
Previous work on gene expression analysis based on RNA sequencing identified a variety of differentially expressed cDNA fragments in the genic male sterile-fertile line 114AB of Capsicum annuum L. In this work, we examined the accumulation of one of the transcript-derived fragments (TDFs), CaMF3 (male fertile 3), in the flower buds of a fertile line. The full genomic DNA sequence of CaMF3 was 1,951 bp long and contained 6 exons and 5 introns, with the complete sequence encoding a putative 25.89 kDa protein of 234 amino acids. The predicted protein of CaMF3 shared sequence similarity with members of the isoamyl acetate-hydrolyzing esterase (IAH1) protein family. CaMF3 expression was detected only in flower buds at stages 7 and 8 and in open flowers of a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anther-specific; CaMF3; Capsicum annuum L. genic male sterility; IAH1.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500016

Evaluation of gene selection metrics for tumor cell classification
Provedor de dados: Genetics and Molecular Biology
Autores: Faceli,Katti; Carvalho,André C.P.L.F. de; Silva Jr,Wilson A.
Gene expression profiles contain the expression level of thousands of genes. Depending on the issue under investigation, this large amount of data makes analysis impractical. Thus, it is important to select subsets of relevant genes to work with. This paper investigates different metrics for gene selection. The metrics are evaluated based on their ability in selecting genes whose expression profile provides information to distinguish between tumor and normal tissues. This evaluation is made by constructing classifiers using the genes selected by each metric and then comparing the performance of these classifiers. The performance of the classifiers is evaluated using the error rate in the classification of new tissues. As the dataset has few tissue samples,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene selection; Machine learning; Gene expression; Sage.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400029

Genetic characterization of Bhutanese native chickens based on an analysis of Red Junglefowl (Gallus gallus gallus and Gallus gallus spadecieus), domestic Southeast Asian and commercial chicken lines (Gallus gallus domesticus)
Provedor de dados: Genetics and Molecular Biology
Autores: Dorji,Nedup; Duangjinda,Monchai; Phasuk,Yupin.
The genetic diversity of Bhutanese chickens needs to be understood in order to develop a suitable conservation strategy for these birds in Bhutan. In this, work, we used microsatellite markers to examine the genetic diversity of Bhutanese chickens. Four Bhutanese chicken varieties (Black plumage, Frizzle, Naked neck and Red Junglefowllike, corresponding to Yuebjha Narp, Phulom, Khuilay and Seim, respectively), two subspecies of Red Junglefowl (Gallus gallus gallus and Gallus gallus spadecieus), two varieties of Thai native chickens (Pradhu Hang Dam and Chee; Gallus gallus domesticus) representing the Southeast Asian domestic chicken, and two commercial lines (Broiler and Single Comb White Leghorn) were genotyped with 18 microsatellites that included 16...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Genetic comparisons; Genetic variability; Microsatellites.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400010

Analysis of general and specific combining abilities of popcorn populations, including selfed parents
Provedor de dados: Genetics and Molecular Biology
Autores: Viana,José Marcelo Soriano; Matta,Frederico de Pina.
Estimation of general and specific combining ability effects in a diallel analysis of cross-pollinating populations, including the selfed parents, is presented in this work. The restrictions considered satisfy the parametric values of the GCA and SCA effects. The method is extended to self-pollinating populations (suitable for other species, without the selfed parents). The analysis of changes in population means due to inbreeding (sensitivity to inbreeding) also permits to assess the predominant direction of dominance deviations and the relative genetic variability in each parent population. The methodology was used to select popcorn populations for intra- and inter-population breeding programs and for hybrid production, developed at the Federal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diallele cross; Combining ability; Inbreeding depression; Popcorn breeding.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400010

RNAi-mediated silencing of the Bmi-1 gene causes growth inhibition and enhances doxorubicin-induced apoptosis in MCF-7 cells
Provedor de dados: Genetics and Molecular Biology
Autores: Wu,Xiang-mei; Liu,Xing; Bu,You-quan; Sengupta,Joyeeta; Cui,Hong-juan; Yi,Fa-ping; Liu,Tao; Yuan,Chen-fu; Shi,Yan-yan; Song,Fang-zhou.
The oncogene Bmi-1 is a member of the Polycomb group gene family. Its expression is found to be greatly increased in a number of malignant tumors including breast cancer. This could suggest Bmi-1 as a potent therapeutic target. In this study, RNAi was introduced to down-regulate the expression of Bmi-1 in a highly malignant breast adenocarcinoma cell line, MCF-7. A thorough study of the biological behavior and chemosensitivity changes of the MCF-7 cells was carried out in context to the therapeutic potential of Bmi-1. The results obtained indicated that siRNA targeting of Bmi-1 could lead to an efficient and specific inhibition of endogenous Bmi-1 activity. The mRNA and protein expression of Bmi-1 were determined by RT-PCR and Western blot, respectively....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA interference; Bmi-1; Retrovirus vector; Doxorubicin; Breast cancer.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400004

Sympatric occurrence of two cytotypes of Astyanax scabripinnis (Characiformes, Characidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Maistro,Edson Luis; Oliveira,Claudio; Foresti,Fausto.
Among four specimens of Astyanax scabripinnis sympatrically collected from the Tamanduá stream (State of São Paulo, Brazil), three, named cytotype I, had 2n = 50 chromosomes (6M + 26SM + 4ST + 14A) and a fundamental number (FN) of 86, and one specimen, named cytotype II, had 2n = 48 chromosomes (6M + 28SM + 4ST + 10A) and FN = 86. Besides the difference in diploid number, the cytotypes showed a clear difference in the distribution of constitutive heterochromatin. One NOR-bearing chromosome pair was detected in both cytotypes and one specimen of cytotype I had multiple NORs (tree chromosome pairs). Robertsonian translocations and an increase or loss of heterochromatin are proposed to explain the karyotypic divergence observed. Some aspects related to the...
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200021

Interaction between estrogen receptor and retinol-binding protein-4 polymorphisms as a tool for the selection of prolific pigs
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Iara Denise Vasconcellos; Gonçalves,Paulo Bayard Dias; Silva,Janandra Cortese da; Portela Jr,Valério Valdetar; Borges,Luiz Felipe Kruel; Oliveira,João Francisco Coelho; Lovatto,Paulo Alberto.
The aim of the present study was to investigate the association of the estrogen receptor (ER-PvuII) and retinol-binding protein 4 (RBP4-MspI) gene polymorphisms and their interactions with prolificacy in a commercial synthetic pig line reared in Brazil. A total of 10,374 piglet records from 218 sows and 817 litters were used for litter size analysis. Only females with three or four farrowings were included in the analysis. The mean litter size ranged from 5.0 to 19.5 piglets. DNA was extracted from leukocytes by a standard method, and ER-PvuII and RBP4-MspI polymorphisms were characterized by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The association between alleles or genotypes and reproductive performance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swine; Marker-assisted selection; Retinol-binding protein-4; Estrogen receptor.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300014

Metatranscriptomic analysis of small RNAs present in soybean deep sequencing libraries
Provedor de dados: Genetics and Molecular Biology
Autores: Molina,Lorrayne Gomes; Cordenonsi da Fonseca,Guilherme; Morais,Guilherme Loss de; de Oliveira,Luiz Felipe Valter; Carvalho,Joseane Biso de; Kulcheski,Franceli Rodrigues; Margis,Rogerio.
A large number of small RNAs unrelated to the soybean genome were identified after deep sequencing of soybean small RNA libraries. A metatranscriptomic analysis was carried out to identify the origin of these sequences. Comparative analyses of small interference RNAs (siRNAs) present in samples collected in open areas corresponding to soybean field plantations and samples from soybean cultivated in greenhouses under a controlled environment were made. Different pathogenic, symbiotic and free-living organisms were identified from samples of both growth systems. They included viruses, bacteria and different groups of fungi. This approach can be useful not only to identify potentially unknown pathogens and pests, but also to understand the relations that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Next generation sequencing; Small RNA; SiRNA; Molecular markers.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200010

Uniparental ancestry markers in Chilean populations
Provedor de dados: Genetics and Molecular Biology
Autores: Vieira-Machado,Camilla Dutra; Tostes,Maluah; Alves,Gabrielle; Nazer,Julio; Martinez,Liliana; Wettig,Elisabeth; Pizarro Rivadeneira,Oscar; Diaz Caamaño,Marcela; Larenas Ascui,Jessica; Pavez,Pedro; Dutra,Maria da Graça; Castilla,Eduardo Enrique; Orioli,Ieda Maria.
Abstract The presence of Native Americans, Europeans, and Africans has led to the development of a multi-ethnic, admixed population in Chile. This study aimed to contribute to the characterization of the uniparental genetic structure of three Chilean regions. Newborns from seven hospitals in Independencia, Providencia, Santiago, Curicó, Cauquenes, Valdívia, and Puerto Montt communes, belonging to the Chilean regions of Santiago, Maule, and Los Lagos, were studied. The presence of Native American mitochondrial DNA (mtDNA) haplogroups and two markers present in the non-recombinant region of the Y chromosome, DYS199 and DYS287, indicative of Native American and African ancestry, respectively, was determined. A high Native American matrilineal contribution and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Uniparental markers; Ancestry; MtDNA; Y-chromosome; ECLAMC.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400573

Mutations of the phenylalanine hydroxylase gene in patients with phenylketonuria in Shanxi, China
Provedor de dados: Genetics and Molecular Biology
Autores: Zhou,Yong-An; Ma,Yun-Xia; Zhang,Quan-Bin; Gao,Wei-Hua; Liu,Jian-Ping; Yang,Jian-Ping; Zhang,Gai-Xiu; Zhang,Xiao-Gang; Yu,Liang.
The variation in mutations in exons 3, 6, 7, 11 and 12 of the phenylalanine hydroxylase (PAH) gene was investigated in 59 children with phenylketonuria (PKU) and 100 normal children. Three single nucleotide polymorphisms were detected by sequence analysis. The mutational frequencies of cDNA 696, cDNA 735 and cDNA 1155 in patients were 96.2%, 76.1% and 7.6%, respectively, whereas in healthy children the corresponding frequencies were 97.0%, 77.3% and 8.3%. In addition, 81 mutations accounted for 61.0% of the mutant alleles. R111X, H64 &gt; TfsX9 and S70 del accounted for 5.1%, 0.8% and 0.8% mutation of alleles in exon 3, whereas EX6-96A &gt; G accounted for 10.2% mutation of alleles in exon 6. R243Q had the highest incidence in exon 7 (12.7%), followed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene mutation; Phenylalanine hydroxylase; Phenylketonuria.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500001

The cytotoxic and growth inhibitory effects of palladium(II) complexes on MDA-MB-435 cells
Provedor de dados: Genetics and Molecular Biology
Autores: Campanella,Nathália Cristina; Demartini,Mariana da Silva; Torres,Claudia; Almeida,Eduardo Tonon de; Gouvêa,Cibele Marli Cação Paiva.
The antitumorigenic potential of two palladium(II) complexes, [Pd(ca2-o-phen)Cl2 ] - C1 and [Pd(dmba)(dppp)Cl] - C2, was evaluated, using MDA-MB-435 cells, a human breast adenocarcinoma cell-line that does not express the estrogen receptor α (ER-). Growth inhibition and induced alterations in cell-morphology were analyzed. The sulforhodamine B test showed that, compared to control cells, both C1 and C2 significantly inhibited (p &lt; 0.5) cell growth. The maximum effect with both was achieved with 1 µM complexes, after 24 h of treatment. No further cell-growth inhibition was achieved by increasing concentration or incubation time. Cell morphology was analyzed after staining with hematoxylin-eosin (HE). The morphological changes noted in the treated cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adenocarcinoma cells; Antitumor; Breast cancer; Chemotherapy; Coordination complexes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100023

A simple method for the detection of recombinogenic substances in filamentous fungi
Provedor de dados: Genetics and Molecular Biology
Autores: Baracho,Marta dos Santos; Baracho,Ivanhoé Rodrigues.
Several tests are available for detecting mutagenic and recombinogenic products in fungi. Since fungi are eukaryotic, these tests permit the detection of substances that produce chromosomal aberrations, or that are recombinogenic. We have developed a new method for testing substances that affect fungal stability using a duplication strain of Aspergillus nidulans and circular statistics for data analysis. The fungus was inoculated on the center of Petri dishes and the substance to be tested was applied to a defined area of the dish. Position of resulting sectors was measured by an angle and the data were analyzed by the Rayleigh test. Extensive testing with different compounds showed this method to be rapid and efficient for screening recombinogenic...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200042

Genetic diversity and structure of Atta robusta (Hymenoptera, Formicidae, Attini), an endangered species endemic to the restinga ecoregion
Provedor de dados: Genetics and Molecular Biology
Autores: Reis,Evelyze Pinheiro dos; Salomão,Tânia Maria Fernandes; Campos,Lucio Antonio de Oliveira; Tavares,Mara Garcia.
The genetic diversity and structure of the ant Atta robusta were assessed by ISSR (inter-simple sequence repeats) in 72 colonies collected from 10 localities in the Brazilian states of Espírito Santo (48 colonies) and Rio de Janeiro (24 colonies). The ISSR pattern included 67 bands, 51 of them (76.1%) polymorphic. Analysis of molecular variance (AMOVA) revealed a high level (57.4%) of inter-population variation, which suggested a high degree of genetic structure that was confirmed by UPGMA (unweighted pair-group method using an arithmetic average) cluster analysis. The significant correlation between genetic and geographic distances (r = 0.64, p &lt; 0.05) indicated isolation that reflected the distance between locations. Overall, the populations were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endemism; Endangered; ISSR; Population genetics; Restinga.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400015

Mitochondrial control region genetic diversity and maternal ancestry of a Brangus-Ibage cattle populations
Provedor de dados: Genetics and Molecular Biology
Autores: Henkes,Luiz Ernani; Silva Jr,Wilson Araújo; Moraes,José Carlos Ferrugem; Weimer,Tania Azevedo.
The genetic diversity of 277 nucleotides in the mitochondrial DNA control region (nt 15,964 to 16,240 in reference sequence) was analyzed in crossbreed beef cattle (Brangus-Ibage, 5/8 Bos primigenius taurus x 3/8 Bos primigenius indicus) as well as in some Nellore samples (B. p. indicus). Fifty-seven mutations were found in Brangus-Ibage comprising 18 haplotypes (haplotype diversity, h = 0.851 ± 0.041 and nucleotide diversity, ntd = 0.009 ± 0.006) and 66 in Nellore (h = 1.00 ± 0.27, ntd = 0.014 ± 0.012). These data indicated sequence identities of 99.6 and 92.1% between the B. p. taurus' reference sequence and Brangus-Ibage and Nellore, respectively. The comparison of our data with sequence data for 612 individuals recovered from GenBank showed a total of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine mtDNA; Maternal lineage; Sequence analysis; Beef cattle; Genetic diversity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100011

Fusion of the subunits α and β of succinyl-CoA synthetase as a phylogenetic marker for Pezizomycotina fungi
Provedor de dados: Genetics and Molecular Biology
Autores: Koire,Amanda M.; Cavalcanti,Andre R.O.
Gene fusions, yielding the formation of multidomain proteins, are evolutionary events that can be utilized as phylogenetic markers. Here we describe a fusion gene comprising the α and β subunits of succinyl-coA synthetase, an enzyme of the TCA cycle, in Pezizomycotina fungi. This fusion is present in all Pezizomycotina with complete genome sequences and absent from all other organisms. Phylogenetic analysis of the α and β subunits of succinyl-CoA synthetase suggests that both subunits were duplicated and retained in Pezizomycotina while one copy was lost from other fungi. One of the duplicated copies was then fused in Pezizomycotina. Our results suggest that the fusion of the α and β subunits of succinyl-CoA synthetase can be used as a molecular marker for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fused genes; Fungi phylogeny; Evolutionary marker.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400020

Bringing to light the molecular evolution of GUX genes in plants
Provedor de dados: Genetics and Molecular Biology
Autores: Gallinari,Rafael Henrique; Coletta,Rafael Della; Araújo,Pedro; Menossi,Marcelo; Nery,Mariana Freitas.
Abstract Hemicellulose and cellulose are essential polysaccharides for plant development and major components of cell wall. They are also an important energy source for the production of ethanol from plant biomass, but their conversion to fermentable sugars is hindered by the complex structure of cell walls. The glucuronic acid substitution of xylan (GUX) enzymes attach glucuronic acid to xylan, a major component of hemicellulose, decreasing the efficiency of enzymes used for ethanol production. Since loss-of-function gux mutants of Arabidopsis thaliana enhance enzyme accessibility and cell wall digestion without adverse phenotypes, GUX genes are potential targets for genetically improving energy crops. However, comprehensive identification of GUX in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: GUX; Sugarcane; Phylogeny; Angiosperms; Biofuels.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100602

A study of the constitutive heterochromatin and nucleolus organizer regions of Isocopris inhiata and Diabroctis mimas (Coleoptera: Scarabaeidae, Scarabaeinae) using C-banding, AgNO3 staining and FISH techniques
Provedor de dados: Genetics and Molecular Biology
Autores: Bione,Edgar; Camparoto,Marjori Leiva; Simões,Zilá Luz Paulino.
Meiotic and mitotic chromosomes of Isocopris inhiata and Diabroctis mimas were studied by standard staining procedures, C-banding, silver nitrate staining and FISH using Apis mellifera 28S rDNA as probe. Isocopris inhiata presented a 2n = 18 (8II+ Xy p) karyotype, composed of meta-submetacentric chromosomes with gradual reduction in size. The karyotype of D. mimas was 2n = 20 (9II+ Xy p), composed of meta-submetacentric (pairs 1, 2, 3, 4 and 7) and acrocentric (pairs 5, 6, 8 and 9) chromosomes, with gradual reduction in size. Analysis of constitutive heterochromatin revealed similar C-banding patterns for both species, showing pericentromeric and telomeric bands and diphasic chromosomes. In addition, the X chromosomes of these species were found to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coleoptera; Heterochromatin; NORs; FISH.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100019

Deafness, palmoplantar hyperkeratosis, and knuckle pads with male-to-male transmission: Bart-Pumphrey syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Gisele Viana de; Steiner,Carlos Eduardo; Cintra,Maria Letícia; Marques-de-Faria,Antonia Paula.
We report on a 22-year-old male patient and his father, both presenting with congenital sensorineural deafness, diffuse palmoplantar keratoderma and knuckle pads. These findings are similar to those previously described in the Bart-Pumphrey syndrome, a rare autosomal dominant disorder. Several conditions including keratoderma and deafness are briefly reviewed.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Deafness; Knuckle pads; Palmoplantar keratoderma; Hyperkeratosis; Bart-Pumphrey syndrome.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200004

Spatiotemporal transcription of the P element and the 412 retrotransposon during embryogenesis of Drosophila melanogaster and D. willistoni
Provedor de dados: Genetics and Molecular Biology
Autores: Blauth,Monica Laner; Bruno,Rafaela Vieira; Abdelhay,Eliana; Valente,Vera Lúcia Silva.
Transposable elements (TEs) are mobile nucleotide sequences which, through changing position in host genomes, partake in important evolutionary processes. The expression patterns of two TEs, P element transposon and 412 retrotransposon, were investigated during Drosophila melanogaster and D. willistoni embryogenesis, by means of embryo hybridization using riboprobes. Spatiotemporal transcription patterns for both TEs were similar to those of developmental genes. Although the two species shared the same P element transcription pattern, this was not so with 412 retrotransposon. These findings suggest that the regulatory sequences involved in the initial development of Drosophila spp are located in the transposable element sequences, and differences, such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila P element; 412 transposable element embryonic development.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400026

Partial duplication of chromosome 20(pter-&gt;q12)
Provedor de dados: Genetics and Molecular Biology
Autores: Della-Rosa,Valter Augusto; Vianna-Morgante,Angela M.
Partial duplication of chromosome 20 (20pter-&gt;20q12) resulting from a maternally inherited translocation t(14;20)(q11;q13) is described in a female child with neuropsychomotor retardation and multiple congenital anomalies. To our knowledge this is the largest duplication of chromosome 20 that includes segments of both the short and the long arms thus far described in a live-born child.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300008

Abnormal spindle orientation during microsporogenesis in an interspecific Brachiaria (Gramineae) hybrid
Provedor de dados: Genetics and Molecular Biology
Autores: Mendes-Bonato,Andréa Beatriz; Pagliarini,Maria Suely; Valle,Cacilda Borges do.
This paper reports a case of abnormal spindle orientation during microsporogenesis in an interspecific hybrid of the tropical grass Brachiaria. In the affected plant, prophase I was normal. In metaphase I, bivalents were regularly co-oriented but distantly positioned and spread over the equatorial plate. In anaphase I, chromosomes failed to converge into focused poles due to parallel spindle fibers. As a consequence, in telophase I, an elongated nucleus or several micronuclei were observed in each pole. In the second division, the behavior was the same, leading to polyads with several micronuclei. A total of 40% of meiotic products were affected. The use of this hybrid in production systems needing good-quality seeds is discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachiaria; Interspecific hybrid; Meiosis; Spindle abnormality; Tropical grass.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100023

Lysosomal diseases: Overview on current diagnosis and treatment
Provedor de dados: Genetics and Molecular Biology
Autores: Poswar,Fabiano de Oliveira; Vairo,Filippo; Burin,Maira; Michelin-Tirelli,Kristiane; Brusius-Facchin,Ana Carolina; Kubaski,Francyne; Souza,Carolina Fischinger Moura de; Baldo,Guilherme; Giugliani,Roberto.
Abstract Lysosomal diseases (LDs), also known as lysosomal storage diseases (LSDs), are a heterogeneous group of conditions caused by defects in lysosomal function. LDs may result from deficiency of lysosomal hydrolases, membrane-associated transporters or other non-enzymatic proteins. Interest in the LD field is growing each year, as more conditions are, or will soon be treatable. In this article, we review the diagnosis of LDs, from clinical suspicion and screening tests to the identification of enzyme or protein deficiencies and molecular genetic diagnosis. We also cover the treatment approaches that are currently available or in development, including hematopoietic stem cell transplantation, enzyme replacement therapy, small molecules, and gene therapy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lysosomal storage diseases; Neonatal screening; Hematopoietic stem cell transplantation; Enzyme replacement therapy; Gene therapy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200165

Hb H disease resulting from the association of an αº-thalassemia allele [-(α)20.5] with an unstable α-globin variant [Hb Icaria]: first report on the occurrence in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Kimura,Elza M.; Oliveira,Denise M.; Fertrin,Kleber; Pinheiro,Valéria R.; Jorge,Susan E.D.C.; Costa,Fernando F.; Sonati,Maria de Fátima.
Hb H Disease is caused by the loss or inactivation of three of the four functional a-globin genes. Patients present chronic hemolytic anemia and splenomegaly. In some cases, occasional blood transfusions are required. Deletions are the main cause of this type of thalassemia (α-thalassemia). We describe here an unusual case of Hb H disease caused by the combination of a common αº deletion [-(α)20.5] with a rare point mutation (c.427T &gt; A), thus resulting in an elongated and unstable α-globin variant, Hb Icaria, (X142K), with 31 additional amino-acid residues. Very high levels of Hb H and Hb Bart's were detected in the patient's red blood cells (14.7 and 19.0%, respectively). This is the first description of this infrequent association in the Brazilian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hereditary hemoglobinopathies; Alpha-thalassemia; Hb H disease; Hb Icaria.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400007

Mining plant genome browsers as a means for efficient connection of physical, genetic and cytogenetic mapping: an example using soybean
Provedor de dados: Genetics and Molecular Biology
Autores: Belarmino,Luis C.; Oliveira,Ana R. da S.; Brasileiro-Vida,Ana C.; Bortoleti,Kyria C. de A.; Bezerra-Neto,João Pacífico; Abdelnoor,Ricardo V.; Benko-Iseppon,Ana M.
Physical maps are important tools to uncover general chromosome structure as well as to compare different plant lineages and species, helping to elucidate genome structure, evolution and possibilities regarding synteny and colinearity. The increasing production of sequence data has opened an opportunity to link information from mapping studies to the underlying sequences. Genome browsers are invaluable platforms that provide access to these sequences, including tools for genome analysis, allowing the integration of multivariate information, and thus aiding to explain the emergence of complex genomes. The present work presents a tutorial regarding the use of genome browsers to develop targeted physical mapping, providing also a general overview and examples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene families; FISH; BAC; SSR; Aquaporin; Bioinformatics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200015

An in silico analysis of the key genes involved in flavonoid biosynthesis in Citrus sinensis
Provedor de dados: Genetics and Molecular Biology
Autores: Lucheta,Adriano R.; Silva-Pinhati,Ana Carla O.; Basílio-Palmieri,Ana Carolina; Berger,Irving J.; Freitas-Astúa,Juliana; Cristofani,Mariângela.
Citrus species are known by their high content of phenolic compounds, including a wide range of flavonoids. In plants, these compounds are involved in protection against biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. In humans, flavonoid consumption has been related to increasing overall health and fighting some important diseases. The goals of this study were to identify expressed sequence tags (EST) in Citrus sinensis (L.) Osbeck corresponding to genes involved in general phenylpropanoid biosynthesis and the key genes involved in the main flavonoids pathways (flavanones, flavones, flavonols, leucoanthocyanidins, anthocyanins and isoflavonoids). A thorough analysis of all related putative genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenylpropanoids; Sweet orange; Expressed sequence tags (EST); Secondary metabolism.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500010

Distinctively variable sequence-based nuclear DNA markers for multilocus phylogeography of the soybean- and rice-infecting fungal pathogen Rhizoctonia solani AG-1 IA
Provedor de dados: Genetics and Molecular Biology
Autores: Ciampi,Maisa B.; Gale,Liane Rosewich; Lemos,Eliana G. de Macedo; Ceresini,Paulo C.
A series of multilocus sequence-based nuclear DNA markers was developed to infer the phylogeographical history of the Basidiomycetous fungal pathogen Rhizoctonia solani AG-1 IA infecting rice and soybean worldwide. The strategy was based on sequencing of cloned genomic DNA fragments (previously used as RFLP probes) and subsequent screening of fungal isolates to detect single nucleotide polymorphisms (SNPs). Ten primer pairs were designed based on these sequences, which resulted in PCR amplification of 200-320 bp size products and polymorphic sequences in all markers analyzed. By direct sequencing we identified both homokaryon and heterokaryon (i.e. dikaryon) isolates at each marker. Cloning the PCR products effectively estimated the allelic phase from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multilocus genotyping; Polymorphisms; Allelic discrimination; Primer design.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400027

Chromosomal variability in Brazilian species of Anthurium Schott (Araceae): Heterochromatin, polyploidy, and B chromosomes
Provedor de dados: Genetics and Molecular Biology
Autores: Nascimento,Sarah do; Coelho,Marcus Alberto Nadruz; Cordeiro,Joel M. P.; Felix,Leonardo P.
Abstract The genus Anthurium has a Neotropical distribution, with karyotype predominance of x = 15, although some species show disploidy or polyploid variations. The karyotypes of seven species and different populations of Anthurium were analyzed using fluorochrome CMA and DAPI staining. The karyotypes were composed of meta- and submetacentric chromosomes, with numbers varying from 2n = 30 to 2n = 60. Supernumerary euchromatic chromosomes were observed in A. affine, and supernumerary heterochromatic chromosomes were observed in A. gladiifolium and A. petrophilum. Polyploidy was recurrent in the Anthurium species analyzed, with records of 2n = 30 and 60 in different A. pentaphyllum populations. Fluorochrome staining revealed different CMA+ banding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B chromosomes; CMA/DAPI; Cytotaxonomy; Neotropical Araceae.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400635

Genetic diversity and phylogenetic relationships in the rye genus Secale L. (rye) based on Secale cereale microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Shang,Hai-Ying; Wei,Yu-Ming; Wang,Xiao-Rong; Zheng,You-Liang.
The genetic diversity and phylogenetic relationships in the genus Secale L. (rye) was evaluated using 24 Secale cereale microsatellite (SCM) markers. The average polymorphism information content (PIC) value of each microsatellite locus in 30 Secale accessions evaluated was higher than that in 47 cultivated ryes (Secale cereale ssp. cereale). The mean genetic similarity (GS) index in Secale was lower than that in cultivated rye. The highest within-species GS index was observed for S. sylvestre and the lowest for S. strictum, whereas the highest between-species GS index was found between S. cereale and S. vavilovii and the lowest between S. sylvestre and S. cereale. There was no obvious difference in GS levels in the cultivated rye accessions from Asia,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cultivated rye; Genetic diversity; Microsatellite markers; Phylogenetic relationships; Secale L.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400018

Scenario of the spread of the invasive species Zaprionus indianus Gupta, 1970 (Diptera, Drosophilidae) in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Galego,Luís Gustavo da Conceição; Carareto,Claudia Marcia Aparecida.
Zaprionus indianus was first recorded in Brazil in 1999 and rapidly spread throughout the country. We have obtained data on esterase loci polymorphisms (Est2 and Est3), and analyzed them, using Landscape Shape Interpolation and the Monmonier Maximum Difference Algorithm to discover how regional invasion occurred. Hence, it was apparent that Z. indianus, after first arriving in São Paulo state, spread throughout the country, probably together with the transportation of commercial fruits by way of the two main Brazilian freeways, BR 153, to the south and the surrounding countryside, and the BR 116 along the coast and throughout the north-east.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zaprionus indianus; Esterase; Landscape shape interpolation; Monmonier's maximum difference algorithm.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400026

Chromosome damage induced by DNA topoisomerase II inhibitors combined with g-radiation in vitro
Provedor de dados: Genetics and Molecular Biology
Autores: Araújo,Maria Cristina P.; Dias,Francisca da Luz; Cecchi,Andréa O.; Antunes,Lusânia M.G.; Takahashi,Catarina S.
Combined radiation and antineoplastic drug treatment have important applications in cancer therapy. In the present work, an evaluation was made of two known topoisomerase II inhibitors, doxorubicin (DXR) and mitoxantrone (MXN), with g-radiation. The effects of DXR or MXN on g-radiation-induced chromosome aberrations in Chinese hamster ovary (CHO) cells were analyzed. Two concentrations of each drug, 0.5 and 1.0 µg/ml DXR, and 0.02 and 0.04 µg/ml MXN, were applied in combination with two doses of g-radiation (20 and 40 cGy). A significant potentiating effect on chromosomal aberrations was observed in CHO cells exposed to 1.0 µg/ml DXR plus 40 cGy. In the other tests, the combination of g-radiation with DXR or MXN gave approximately additive effects. Reduced...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300021

Carbohydrate metabolism of Xylella fastidiosa: Detection of glycolytic and pentose phosphate pathway enzymes and cloning and expression of the enolase gene
Provedor de dados: Genetics and Molecular Biology
Autores: Facincani,Agda Paula; Ferro,Jesus Aparecido; Pizauro Jr.,João Martins; Pereira Jr.,Haroldo Alves; Lemos,Eliana Gertrudes de Macedo; Prado,Alessandro Luis do; Ferro,Maria Inês T.
The objective of this work was to assess the functionality of the glycolytic pathways in the bacterium Xylella fastidiosa. To this effect, the enzymes phosphoglucose isomerase, aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase of the glycolytic pathway, and glucose 6-phosphate dehydrogenase of the Entner-Doudoroff pathway were studied, followed by cloning and expression studies of the enolase gene and determination of its activity. These studies showed that X. fastidiosa does not use the glycolytic pathway to metabolize carbohydrates, which explains the increased duplication time of this phytopatogen. Recombinant enolase was expressed as inclusion bodies and solubilized with urea (most efficient extractor), Triton X-100, and TCA....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enolase; Entner-Doudoroff; Enzyme; Glycolysis; Xylella fastidiosa.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200015

Prediction of social structure and genetic relatedness in colonies of the facultative polygynous stingless bee Melipona bicolor (Hymenoptera, Apidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Reis,Evelyze Pinheiro dos; Campos,Lucio Antonio de Oliveira; Tavares,Mara Garcia.
Stingless bee colonies typically consist of one single-mated mother queen and her worker offspring. The stingless bee Melipona bicolor (Hymenoptera: Apidae) shows facultative polygyny, which makes this species particularly suitable for testing theoretical expectations concerning social behavior. In this study, we investigated the social structure and genetic relatedness among workers from eight natural and six manipulated colonies of M. bicolor over a period of one year. The populations of M. bicolor contained monogynous and polygynous colonies. The estimated genetic relatedness among workers from monogynous and polygynous colonies was 0.75 ± 0.12 and 0.53 ± 0.16 (mean ± SEM), respectively. Although the parental genotypes had significant effects on genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melipona; Microsatellites; Polygyny; Queen number; Social structure.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200028

Genetic parameters for body weight, carcass chemical composition and yield in a broiler-layer cross developed for QTL mapping
Provedor de dados: Genetics and Molecular Biology
Autores: Nunes,Beatriz do Nascimento; Ramos,Salvador Boccaletti; Savegnago,Rodrigo Pelicioni; Ledur,Mônica Corrêa; Nones,Kátia; Klein,Claudete Hara; Munari,Danísio Prado.
The objective of this study was to estimate genetic and phenotypic correlations of body weight at 6 weeks of age (BW6), as well as final carcass yield, and moisture, protein, fat and ash contents, using data from 3,422 F2 chickens originated from reciprocal cross between a broiler and a layer line. Variance components were estimated by the REML method, using animal models for evaluating random additive genetic and fixed contemporary group (sex, hatch and genetic group) effects. The heritability estimates (h²) for BW6, carcass yield and percentage of carcass moisture were 0.31 ± 0.07, 0.20 ± 0.05 and 0.33 ± 0.07, respectively. The h² for the percentages of protein, fat and ash on a dry matter basis were 0.48 ± 0.09, 0.55 ± 0.10 and 0.36 ± 0.08,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler; Carcass composition; Genetic correlation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300011

X monosomy and balanced Robertsonian translocation in a girl with Turner Syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Aline Lourenço da; Lima,Renata LL Ferreira de; Ribeiro,Lucilene Arilho; Moretti-Ferreira,Danilo.
We describe a case of X monosomy associated with a maternally inherited t(13;14) Robertsonian translocation in a girl with Turner syndrome. The girl's X chromosome was demonstrated to be maternally inherited, ruling out the hypothesis that the translocation exerted an interchromosomal effect on the origin of the monosomy. Chromosomes 13 and 14 showed biparental inheritance.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Robertsonian translocation; X monosomy; Interchromosomal effect.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100010

Characterization analysis and polymorphism detection of the porcine Myd88 gene
Provedor de dados: Genetics and Molecular Biology
Autores: Li,Xinyun; Liu,Huazhen; Shulin,Yang; Tang,Zhonglin; Ma,Yuehui; Chu,Mingxing; Li,Kui.
The myeloid differentiation primary response protein 88 (Myd88) is an essential adaptor protein, which mediates in all Toll-like receptor (TLR) members signal transduction, except for TLR3. In this study, the 4464 bp genomic sequence of porcine Myd88 was first isolated, whereupon tissue distribution, chromosome mapping and single nucleotide polymorphism (SNP) were analyzed. Our results revealed that porcine Myd88 gene, which was located at chromosome 13 linked with marker S0288 (distance = 40 cR; LOD = 8.66), was widely expressed in all the examined tissues. There were 16 potential SNPs in the isolated genome fragment. SNP 797T/C in the first intron was studied, with no significant association being found between the genotype and immune traits in pigs (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myd88; TLR; Polymorphism; Pig; Chromosome mapping.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200015

Maternal effects on the genetic evaluation of Tabapuã beef cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Campêlo,José Elivalto Guimarães; Lopes,Paulo Sávio; Torres,Robledo de Almeida; Silva,Luiz Otávio Campos da; Euclydes,Ricardo Frederico; Araújo,Cláudio Vieira de; Pereira,Carmen Silva.
The aim of this investigation was to study the influence of maternal effects on the genetic evaluation of sire production in Tabapuã beef cattle. Single and multiple trait analyses were done with adjusted animal weights at 120, 240 and 420 days of age. Antagonism was observed between additive direct and maternal genetic effects, with the maternal effect being higher until weaning. The inclusion of maternal effects in the models removed part of the additive variance only in single trait analyses and resulted in smaller means and standard deviations for the sire breeding values. The use of maternal effect associated with single or multiple traits may affect sire ranking. The contradictory results of the single and multiple trait analyses for additive direct...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Additive direct effects; Beef cattle; Genetic parameters; Growth; Maternal effects.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400009

Genetic evidence for a species complex within the piranha Serrasalmus maculatus (Characiformes, Serrasalmidae) from three Neotropical river basins based on mitochondrial DNA sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Bignotto,Thaís Souto; Maniglia,Thiago Cintra; Gomes,Vivian Nunes; Oliveira,Isadora Janolio de; Agostinho,Carlos Sérgio; Prioli,Sônia Maria Alves Pinto; Prioli,Alberto José.
Abstract Mitochondrial molecular markers (DNA sequences of D-loop, cytochrome b and cytochrome c oxidase I) were employed to characterize populations of the piranha Serrasalmus maculatus from Upper Paraná, Upper Paraguay and Tocantins River basins. D-loop sequences of S. maculatus population from Paraná-Paraguay River basin exhibited tandem repeats of short motifs (12 base pairs) and variable numbers depending on specimens, accounting for length variation. Concatenated mitochondrial sequences suggested that S. maculatus encompasses different mitochondrial DNA lineages. Although sampling was restricted to three river basins, phylogenetic analysis clearly indicated that the species currently recognized as S. maculatus presents high genetic variability....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptic species; Species delimitation; D-loop; Cytochrome b; Cytochrome c oxidase I.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100801

Eucalyptus ESTs associated with resistance to herbicide inhibitors of aromatic and branched-chain amino acid synthesis
Provedor de dados: Genetics and Molecular Biology
Autores: Velini,Edivaldo Domingues; Trindade,Maria Lúcia Bueno; Alves,Elza; Catâneo,Ana Catarina; Marino,Celso Luis; Maia,Ivan de Godoy; Mori,Edson Seizo; Furtado,Edson Luiz; Guerrini,Iraê Amaral; Wilcken,Carlos Frederico.
Herbicides inhibit enzymatic systems of plants. Acetolactate synthase (ALS, EC = 4.1.3.18) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS, EC 2.5.1.19) are key enzymes for herbicide action. Hundreds of compounds inhibit ALS. This enzyme is highly variable, enabling the selective control of weeds in a number of crops. Glyphosate, the only commercial herbicide inhibiting EPSPS is widely used for non-selective control of weeds in many crops. Recently, transgenic crops resistant to glyphosate were developed and have been used by farmers. The aim of this study was the data mining of eucalypt expressed sequence tags (ESTs) in the FORESTs Genome Project database (&lt;A HREF="https://forests.esalq.usp.br"&gt;https://forests.esalq.usp.br&lt;/A&gt;) related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus; Herbicides; ALS; EPSPS.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400012

Equine protease inhibitor system as a marker for the diagnosis of chronic obstructive pulmonary disease (COPD)
Provedor de dados: Genetics and Molecular Biology
Autores: Vinocur,Myriam E.; Brass,Karin E.; Caetano,Alexandre R.; Silva,Letícia F.; Silva,Aline C.; Silva,Carlos A.M.
The protease inhibitor system (PI) was investigated to ascertain if it can be used as a marker of chronic obstructive pulmonary disease (COPD) in thoroughbred horses. Serum samples were taken from healthy thoroughbreds (n = 13) and those diagnosed as having COPD (n = 24) or inflammatory airway disease (IAD, n = 38) as well as from 3,600 undiagnosed thoroughbred horses. PI allelic and genotypic frequencies were estimated using protein electrophoresis on starch and polyacrylamide gels. The four groups of horses showed high genotypic similarity and none of the observed alleles or genotypes of the equine PI system were found to be associated with COPD.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Horse; Chronic obstructive pulmonary disease; Protease inhibitor system.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300007

Successful crosses between fungal-resistant wild species of Arachis (section Arachis) and Arachis hypogaea
Provedor de dados: Genetics and Molecular Biology
Autores: Fávero,Alessandra Pereira; Santos,Rodrigo Furtado dos; Simpson,Charles E.; Valls,José Francisco Montenegro; Vello,Natal Antonio.
Peanut (Arachis hypogaea) is the fifth most produced oil crop worldwide. Besides lack of water, fungal diseases are the most limiting factors for the crop. Several species of Arachis are resistant to certain pests and diseases. This study aimed to successfully cross the A-genome with B-K-A genome wild species previously selected for fungal disease resistance, but that are still untested. We also aimed to polyplodize the amphihaploid chromosomes; cross the synthetic amphidiploids and A. hypogaea to introgress disease resistance genes into the cultivated peanut; and analyze pollen viability and morphological descriptors for all progenies and their parents. We selected 12 A-genome accessions as male parents and three B-genome species, one K-genome species,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peanut; Interspecific hybrids; Polyploidization; Colchicine; Pollen viability.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300353

Comparative karyotype analysis of populations in the Alstroemeria presliana Herbert (Alstroemeriaceae) complex in Chile
Provedor de dados: Genetics and Molecular Biology
Autores: Baeza,Carlos; Finot,Víctor L.; Ruiz,Eduardo.
Alstroemeria L., one of the most diverse genera of the Chilean flora and of high floricultural value, is represented by 35 species, most of them distributed between 28–38° S in the Mediterranean zone of Central Chile. There are 24 complex-forming taxa, of which 18 have conservation problems (8 are considered “endangered” and 10 as “vulnerable”). One of these complexes is Alstroemeria presliana Herb. with two subspecies: subsp. presliana and subsp. australis Bayer. Alstroemeria presliana grows in Chile and Argentina: subsp. presliana is distributed from Reserva Nacional Siete Tazas (35°27′ S, Region of Maule) to Antuco, (37°25′ S, Region of Bío-Bío), and is also found in Neuquén, Argentina; subsp. australis is endemic to the Cordillera of Nahuelbuta. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alstroemeria; Asymmetry; Chile; Karyotype; Species complex.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200199

Population and mutation analysis of Y-STR loci in a sample from the city of São Paulo (Brazil)
Provedor de dados: Genetics and Molecular Biology
Autores: Soares-Vieira,José A.; Billerbeck,Ana E.C.; Iwamura,Edna S.M.; Mendonca,Berenice B.; Gusmão,Leonor; Otto,Paulo A.
The haplotypes of seven Y-chromosome STR loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, and DYS393) were determined in a sample of 634 healthy Brazilian males (190 adult individuals and 222 father-son pairs). The 412 adults were unrelated, and the 222 father-son pairs had their biological relationship confirmed using autosomal STRs (LR &gt; 10,000). Among the 412 adults, a total of 264 different 7-loci haplotypes were identified, 210 of which were unique. The most frequent haplotype was detected in 31 instances, occurring with a frequency of 7.52%. The haplotype diversity index was calculated as 98.83%. Upon transmission of the 1,554 alleles, in 222 father-son pairs, six mutations were observed, with an average overall rate of 3.86 x 10-3 per...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Y-STR population data; São Paulo (Brazil); Mutation rates; Duplications.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400009

Population structure and genetic variability of mainland and insular populations of the Neotropical water rat, Nectomys squamipes (Rodentia, Sigmodontinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Almeida,Francisca C.; Maroja,Luana S.; Moreira,Miguel A.M.; Seuánez,Héctor N.; Cerqueira,Rui.
Seven microsatellite loci were used to investigate the genetic variability and structure of six mainland and two island populations of the Neotropical water rat Nectomys squamipes, a South American semi-aquatic rodent species with a wide distribution. High levels of variability were found within mainland populations while island populations were less variable but the more differentiated in respect to allele number and frequency. The time of biological divergence between mainland and island populations coincided with geological data. A significant geographic structure was found in mainland populations (theta = 0.099; rho = 0.086) although the degree of differentiation was relatively low in respect to the distance between surveyed localities (24 to 740 km)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Population genetics; Islands; Population expansion; Population size; Divergence time.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500008

Genetic variability of Herpailurus yagouaroundi, Puma concolor and Panthera onca (Mammalia, Felidae) studied using Felis catus microsatellites
Provedor de dados: Genetics and Molecular Biology
Autores: Moreno,Vanessa Roma; Grisolia,Alexéia Barufatti; Campagnari,Francine; Milazzotto,Marcela; Adania,Cristina Harumi; Garcia,José Fernando; Souza,Edislane Barreiros de.
We used four microsatellite loci (Fca08, Fca45, Fca77 and Fca96) from the domestic cat, Felis catus, to investigate genetic variability in specimens of Herpailurus yagouaroundi (jaguarundi, otter cat, eyra), Puma concolor (cougar, mountain lion, puma) and Panthera onca (jaguar) held in various Brazilian zoos. Samples of DNA from the cats were PCR amplified and then sequenced before being analyzed using the CERVUS program. Our results show a mean polymorphic information content (PIC) of 0.83 for H. yagouaroundi, 0.66 for P. concolor and 0.69 for P. onca and a mean of 10.3 alleles for the Fca08 locus, 5.3 for Fca 45, 9 for Fca 77 and 14 for Fca 96. These results indicate a relatively high level of genetic diversity for the specimens studied.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Mammals; Felines; Microsatellites.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200017

Temperature-dependent gonadal hybrid dysgenesis in Drosophila willistoni
Provedor de dados: Genetics and Molecular Biology
Autores: Regner,Luciana P.; Abdelhay,Eliana; Rohde,Cláudia; Rodrigues,Jaqueline J.S.; Valente,Vera Lucia S.
Temperature-dependent gonadal dysgenesis was shown to occur in the progeny of both inter- and intrastrain crosses involving two populations of Drosophila willistoni, one of which was an old laboratory stock, and the other, freshly collected from a natural population. We propose that the phenomenon observed was caused by the mobilization of transposable elements, as occurs in several other Drosophila species.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200012

Trimming and clustering sugarcane ESTs
Provedor de dados: Genetics and Molecular Biology
Autores: Telles,Guilherme P.; Silva,Felipe R. da.
The original clustering procedure adopted in the Sugarcane Expressed Sequence Tag project (SUCEST) had many problems, for instance too many clusters, the presence of ribosomal sequences, etc. We therefore redesigned the clustering procedure entirely, including a much more careful initial trimming of the reads. In this paper the new trimming and clustering strategies are described in detail and we give the new official figures for the project, 237,954 expressed sequence tags and 43,141 clusters.
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100004

Molecular analysis of the most prevalent mutations of the FANCA and FANCC genes in Brazilian patients with Fanconi anaemia
Provedor de dados: Genetics and Molecular Biology
Autores: Rodriguez,David Enrique Aguilar; Lima,Carmen Silvia Passos; Lourenço,Gustavo Jacob; Figueiredo,Maria Estela; Carneiro,Jorge David Aivazoglu; Tone,Luiz Gonzaga; Llerena Jr.,Juan Clinton; Toscano,Raquel Alves; Brandalise,Silvia; Pinto Júnior,Walter; Costa,Fernando Ferreira; Bertuzzo,Carmen Sílvia.
Fanconi anaemia (FA) is a recessive autosomal disease determined by mutations in genes of at least eleven complementation groups, with distinct distributions in different populations. As far as we know, there are no reports regarding the molecular characterisation of the disease in unselected FA patients in Brazil. OBECTIVE: This study aimed to investigate the most prevalent mutations of FANCA and FANCC genes in Brazilian patients with FA. METHODS: Genomic DNA obtained from 22 racially and ethnically diverse unrelated FA patients (mean age ± SD: 14.0 ± 7.8 years; 10 male, 12 female; 14 white, 8 black) was analysed by polymerase chain reaction and restriction site assays for identification of FANCA (delta3788-3790) and FANCC (delta322G, IVS4+4A -&gt; T,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fanconi anaemia; DEB test; Molecular diagnosis; FANCA; FANCC.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200004

Polymorphism of Sooty-fronted Spinetail (Synallaxis frontalis Aves: Furnariidae): Evidence of chromosomal rearrangements by pericentric inversion in autosomal macrochromosomes
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Marcelo Santos de; Barcellos,Suziane Alves; Costa,Alice Lemos; Kretschmer,Rafael; Garnero,Analía Del Valle; Gunski,Ricardo José.
Abstract The Passeriformes is the most diverse and cytogenetically well-known clade of birds, comprising approximately 5,000 species. The sooty-fronted spinetail (Synallaxis frontalis Aves: Furnariidae) species, which belongs to the order Passeriformes, is typically found in South America, where it is widely distributed. Polymorphisms provide genetic variability, important for several evolutionary processes, including speciation and adaptation to the environment. The aim of this work was to analyze the possible cytotypes and systemic events involved in the species polymorphism. Of the sampled 19 individuals, two thirds were polymorphic, an event supposedly linked to mutations resulting from genomic evolution that can be transmitted hereditarily. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal polymorphism; Evolution; Cytogenetics.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100062

Analysis of the association between spawning time QTL markers and the biannual spawning behavior in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: Genetics and Molecular Biology
Autores: Colihueque,Nelson; Cárdenas,Rosy; Ramírez,Lorena; Estay,Francisco; Araneda,Cristian.
The rainbow trout is a salmonid fish that occasionally exhibits broodstocks with biannual spawning behavior, a phenomenon known as a double annual reproductive cycle (DARC). Spawning time quantitative trait loci (SPT-QTLs) affect the time of the year that female rainbow trout spawn and may influence expression of the DARC trait. In this study, microsatellite markers linked and unlinked to SPT-QTLs were genotyped to investigate the underlying genetics of this trait. SPT-QTLs influenced the DARC trait since in two case-control comparisons three linked markers (OmyFGT12TUF, One3ASC and One19ASC) had significant levels of allelic frequency differentiation and marker-character association. Furthermore, alleles of One3ASC and One19ASC had significantly higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Association analysis; Biannual spawning; Microsatellite markers; Rainbow trout.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300032

Expression of stress-related genes in zebrawood (Astronium fraxinifolium, Anacardiaceae) seedlings following germination in microgravity
Provedor de dados: Genetics and Molecular Biology
Autores: Inglis,Peter W.; Ciampi,Ana Y.; Salomão,Antonieta N.; Costa,Tânia da S.A.; Azevedo,Vânia C.R.
Seeds of a tropical tree species from Brazil, Astronium fraxinifolium, or zebrawood, were germinated, for the first time in microgravity, aboard the International Space Station for nine days. Following three days of subsequent growth under normal terrestrial gravitational conditions, greater root length and numbers of secondary roots was observed in the microgravity-treated seedlings compared to terrestrially germinated controls. Suppression subtractive hybridization of cDNA and EST analysis were used to detect differential gene expression in the microgravity-treated seedlings in comparison to those initially grown in normal gravity (forward subtraction). Despite their return to, and growth in normal gravity, the subtracted library derived from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microgravity; Stress response; Germination; Suppression subtractive hybridization; Zebrawood.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100014

Comparative genome analysis of proteases, oligopeptide uptake and secretion systems in Mycoplasma spp
Provedor de dados: Genetics and Molecular Biology
Autores: Staats,Charley Christian; Boldo,Juliano; Broetto,Leonardo; Vainstein,Marilene; Schrank,Augusto.
Mycoplasmas are very fastidious in their nutritional requirements for in vitro growth and have limited biosynthetic capacity, a reflection of their reduced genomes. As a result, these bacteria depend upon external metabolites for nutrition and growth and have developed dependence on their hosts for survival and maintenance. Protein degradation and peptide importation play an important role in Mycoplasma spp. nutrition, and proteases can play a role in host adaptation and pathogenicity. Here, we present a general survey on the genes involved in protein degradation, secretion and importation, comparing all available Mollicute genomes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Proteases; Minimal genomes; Secretion systems.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200009

Chromosomal similarity between the Scaly-headed parrot (Pionus maximiliani), the Short-tailed parrot (Graydidascalus brachyurus) and the Yellow-faced parrot (Salvatoria xanthops) (Psittaciformes: Aves): a cytotaxonomic analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Caparroz,Renato; Duarte,José Maurício Barbanti.
Behavior, morphology, allozyme studies and DNA hybridization and sequencing data all suggest the independent evolution of the Old and New World parrots and support tribe status for the American species, although the phylogenetic relationships within this tribe are still poorly understood. A previous study has shown that the Yellow-faced parrot (Amazona xanthops Spix 1824) exhibits large karyotypic differences compared to the other Amazona species and suggested that this species should be renamed Salvatoria xanthops, although the relationships between S. xanthops and the other New World parrots remain unclear. In the present work, we describe the karyotype of the Scaly-headed parrot (Pionus maximiliani, Kuhl 1820) and the karyotype and C-banding pattern of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome; Karyotype; Cytotaxonomy; Psittaciformes; Parrots.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400010

Characterization of two full-sized P elements from Drosophila sturtevanti and Drosophila prosaltans
Provedor de dados: Genetics and Molecular Biology
Autores: Castro,Juliana Polachini de; Carareto,Claudia M.A.
Previously, only partial P element sequences have been reported in the saltans group of Drosophila but in this paper we report two complete P element sequences from Drosophila sturtevanti and Drosophila prosaltans. The divergence of these sequences from the canonical P element of Drosophila melanogaster is about 31% at the nucleotide level. Phylogenetic analysis revealed that both elements belong to a clade of divergent sequences from the saltans and willistoni groups previously described by other authors.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: D. sturtevanti; D. prosaltans; Full-size P element; Phylogeny.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300011

Genetic diversity and prevalence of CCR2-CCR5 gene polymorphisms in the Omani population
Provedor de dados: Genetics and Molecular Biology
Autores: Al-Mahruqi,Samira H.; Zadjali,Fahad; Beja-Pereira,Albano; Koh,Crystal Y.; Balkhair,Abdullah; Al-Jabri,Ali A.
Polymorphisms in the regulatory region of the CCR5 gene affect protein expression and modulate the progress of HIV-1 disease. Because of this prominent role, variations in this gene have been under differential pressure and their frequencies vary among human populations. The CCR2V64I mutation is tightly linked to certain polymorphisms in the CCR5 gene. The current Omani population is genetically diverse, a reflection of their history as traders who ruled extensive regions around the Indian Ocean. In this study, we examined the CCR2-CCR5 haplotypes in Omanis and compared the patterns of genetic diversity with those of other populations. Blood samples were collected from 115 Omani adults and genomic DNA was screened to identify the polymorphic sites in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CCR2; CCR5; Divergence; Haplotype; HIV-1; Oman.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100004

Frequencies of BCR-ABL1 fusion transcripts among Sudanese chronic myeloid leukaemia patients
Provedor de dados: Genetics and Molecular Biology
Autores: Osman,Emad-Aldin I.; Hamad,Kamal; Elmula,Imad M. Fadl; Ibrahim,Muntaser E.
The incidence of one or other rearrangement in chronic myeloid leukemia (CML) patients varies in different reported series. In this study we report the frequencies of BCR-ABL1 fusion transcript variants studied in 43 CML patients from Sudan. The study includes 46 Sudanese patients, three of which negative for the BCR-ABL1 fusion transcript. More than half of 43 positive patients showed b2a2 fusion transcript (53.5%), while (41.9%) showed b3a2 transcript and the remaining (4.6%) coexpression of b3a2/ b2a2 and b3a2/b2a2/e19a2. We detected neither coexpression of p210/p190 nor e1a2 alone. Male patients showed a tendency to express b2a2, while female tende to express b3a2 (p = 0.017). Moreover, a single nucleotide polymorphism was detected in BCR exon 13 in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BCR-ABL; Chronic myeloid leukemia; Ph chromosome; Sudanese; RT-PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200005

Phenotypic variation of erythrocyte linker histone H1.c in a pheasant (Phasianus colchicus L.) population
Provedor de dados: Genetics and Molecular Biology
Autores: Kowalski,Andrzej; Palyga,Jan; Górnicka-Michalska,Ewa; Bernacki,Zenon; Adamski,Marek.
Our goal was to characterize a phenotypic variation of the pheasant erythrocyte linker histone subtype H1.c. By using two-dimensional polyacrylamide gel electrophoresis three histone H1.c phenotypes were identified. The differently migrating allelic variants H1.c1 and H1.c2 formed either two homozygous phenotypes, c1 and c2, or a single heterozygous phenotype, c1c2. In the pheasant population screened, birds with phenotype c2 were the most common (frequency 0.761) while individuals with phenotype c1 were rare (frequency 0.043).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Linker histones; H1.c subtype; Allelic variants; Electrophoresis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300016

Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs)
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Jorge A.G. da.
Expressed sequence tags (ESTs) in the sugarcane (Saccharum spp) database (SUCEST) were electronically searched and 402 microsatellites identified. Various dinucleotide and trinucleotide simple sequence repeat (SSR) motifs were found, with these being more frequently observed in ESTs obtained from flower cDNA libraries. PCR primers were designed for 20 of these SSRs and were tested on eight sugarcane genotypes, the sequences of these primers and a list of known sugarcane genes containing SSR motifs being presented in this paper. Polymorphisms were evident both at the cultivar level and between Saccharum species. These results show that EST-derived SSRs in Saccharum species are useful because they are polymorphic and transferable. The large number of...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100021

Early colonization pattern of maize (Zea mays L. Poales, Poaceae) roots by Herbaspirillum seropedicae (Burkholderiales, Oxalobacteraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Monteiro,Rose A.; Schmidt,Maria A.; Baura,Valter A. de; Balsanelli,Eduardo; Wassem,Roseli; Yates,Marshall G.; Randi,Marco A.F.; Pedrosa,Fábio O.; Souza,Emanuel M. de.
The bacterium Herbaspirillum seropedicae is an endophytic diazotroph found in several plants, including economically important poaceous species. However, the mechanisms involved in the interaction between H. seropedicae and these plants are not completely characterized. We investigated the attachment of Herbaspirillum to maize roots and the invasion of the roots by this bacterium using H. seropedicae strain SMR1 transformed with the suicide plasmid pUTKandsRed, which carries a mini-Tn5 transposon containing the gene for the Discosoma red fluorescent protein (Dsred) constitutively expressed together with the kanamycin resistance gene. Integration of the mini-Tn5 into the bacterial chromosome yielded the mutant H. seropedicae strain RAM4 which was capable of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Confocal microscopy; Diazotroph; Endophytic; Herbaspirillum seropedicae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500021

A novel statistical method for assessing SSR variation in autotetraploid alfalfa (Medicago sativa L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Liu,Zhi-Peng; Liu,Gong-She; Yang,Qing-Chuan.
The level of variation of simple sequence repeat (SSR) markers in cultivated alfalfa from American, Australian and Chinese sources was evaluated using a novel autotetraploid statistical method to calculate the effective number of alleles, the allele frequencies and heterozygosity. We used 19 SSR primers to screen seven polymorphic SSR loci in 320 plants from eight populations. The genetic distance and phylogenetic analysis (DISPAN) program was used to calculate the inter- and intra-population genetic relationships using the conventional binary absence/presence (0/1) method and our novel autotetraploid method. The autotetraploid method resulted in significantly higher heterozygosity (p &lt; 0.01), average effective number (p &lt; 0.01) and lower standard...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autotetraploid; Genetic variation; Medicago sativa L.; SSR; Statistical genetics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300015

Identification of histone methylation modifiers and their expression patterns during somatic embryogenesis in Hevea brasiliensis
Provedor de dados: Genetics and Molecular Biology
Autores: Li,Hui-Liang; Guo,Dong; Zhu,Jia-Hong; Wang,Ying; Peng,Shi-Qing.
Abstract Histone methylation plays a crucial role in various biological processes, from heterochromatin formation to transcriptional regulation. Currently, no information is available regarding histone methylation modifiers in the important rubber-producing plant Hevea brasiliensis. Here, we identified 47 histone methyltransferase (HMT) genes and 25 histone demethylase (HDM) genes as possible members of the histone methylation modifiers in the rubber tree genome. According to the structural features of HMT and HDM, the HbHMTs were classified into two groups (HbPRMs and HbSDGs), the HbHDMs have two groups (HbLSDs and HbJMJs). Expression patterns were analyzed in five different tissues and at different phases of somatic embryogenesis. HbSDG10, 21, 25, 33,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hevea brasiliensis; Histone methylation; Histone methyltransferase; Histone demethylase; Somatic embryogenesis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100301

Genomic resources for the conservation and management of the harpy eagle (Harpia harpyja, Falconiformes, Accipitridae)
Provedor de dados: Genetics and Molecular Biology
Autores: Banhos,Aureo; Hrbek,Tomas; Gravena,Waleska; Sanaiotti,Tânia; Farias,Izeni P.
We report the characterization and optimization of 45 heterologous microsatellite loci, and the development of a new set of molecular sex markers for the conservation and management of the Neotropical harpy eagle (Harpia harpyja L. 1758). Of the 45 microsatellites tested, 24 were polymorphic, six monomorphic, 10 uncharacterizable due to multiple bands and five did not amplify. The observed gene diversity of the analyzed sample of H. harpyja was low and similar to that of other threatened Falconiformes. While a high proportion of the microsatellite markers were highly variable, individuals of H. harpyja could be differentiated by a joint analysis of just three (p = 2.79 x 10-4) or four markers (p = 2.89 x 10-5). Paternity could be rejected with 95.23% and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Harpia harpyja; Microsatellites; Raptors; Sex markers.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100025

Evolutionary analysis of apolipoprotein E by Maximum Likelihood and complex network methods
Provedor de dados: Genetics and Molecular Biology
Autores: Benevides,Leandro de Jesus; Carvalho,Daniel Santana de; Andrade,Roberto Fernandes Silva; Bomfim,Gilberto Cafezeiro; Fernandes,Flora Maria de Campos.
Abstract Apolipoprotein E (apo E) is a human glycoprotein with 299 amino acids, and it is a major component of very low density lipoproteins (VLDL) and a group of high-density lipoproteins (HDL). Phylogenetic studies are important to clarify how various apo E proteins are related in groups of organisms and whether they evolved from a common ancestor. Here, we aimed at performing a phylogenetic study on apo E carrying organisms. We employed a classical and robust method, such as Maximum Likelihood (ML), and compared the results using a more recent approach based on complex networks. Thirty-two apo E amino acid sequences were downloaded from NCBI. A clear separation could be observed among three major groups: mammals, fish and amphibians. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein E; Phylogeny; Complex network; Maximum Likelihood.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400665

Skull shape and size variation within and between mendocinus and torquatus groups in the genus Ctenomys (Rodentia: Ctenomyidae) in chromosomal polymorphism context
Provedor de dados: Genetics and Molecular Biology
Autores: Fornel,Rodrigo; Cordeiro-Estrela,Pedro; de Freitas,Thales Renato O.
Abstract We tested the association between chromosomal polymorphism and skull shape and size variation in two groups of the subterranean rodent Ctenomys. The hypothesis is based on the premise that chromosomal rearrangements in small populations, as it occurs in Ctenomys, produce reproductive isolation and allow the independent diversification of populations. The mendocinus group has species with low chromosomal diploid number variation (2n=46-48), while species from the torquatus group have a higher karyotype variation (2n=42-70). We analyzed the shape and size variation of skull and mandible by a geometric morphometric approach, with univariate and multivariate statistical analysis in 12 species from mendocinus and torquatus groups of the genus Ctenomys....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cranium; Geometric morphometrics; Phenotypic evolution; Subterranean rodent.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200263

Molecular characterization of Indian pathotypes of Puccinia striiformis f. sp. tritici and multigene phylogenetic analysis to establish inter- and intraspecific relationships
Provedor de dados: Genetics and Molecular Biology
Autores: Aggarwal,Rashmi; Kulshreshtha,Deepika; Sharma,Sapna; Singh,Vaibhav K.; Manjunatha,Channappa; Bhardwaj,Subhash C.; Saharan,Mahender S.
Abstract Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is one of the most devastating diseases of wheat (Triticum spp.) worldwide. Indian isolates were characterised based on their phenotypic reaction on differential hosts carrying different Yr genes. Based on virulence/avirulence structure, isolates were characterised into ten different pathotypes viz. 70S0-2, 67S64, 70S4, 66S0, 70S64, 66S64-1, 38S102, 47S102, 46S119, and 78S84. These Indian pathotypes of P. striiformis f. sp. tritici and 38 pathotypes of other rust species (P. graminis tritici and P. triticina) were used in this study to analyze their molecular phylogenetic relationship. The nucleotides of rDNA-ITS, partial β-tubulin and ketopantoate reductase genes of all the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; Stripe rust; Virulence; Diversity; Phylogeny.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500834

Somatic and germ cell cytogenetic studies and AZF microdeletion screening in infertile men
Provedor de dados: Genetics and Molecular Biology
Autores: Carrara,Rita C. V.; Yamasaki,Rui; Mazucatto,Luís F.; Veludo,Maria A. Llorach; Sartorato,Edi L.; Pina-Neto,João M.
Clinical and cytogenetic studies were performed in 65 infertile individuals, and 56 of them were also screened for microdeletions in Yq11 (AZF region). Relevant environmental etiological factors were identified in 10 cases (15.4%). Sertoli-cell-only syndrome was diagnosed in six patients (9,2%). Karyotype abnormalities were detected in six individuals, and five other patients presented desynapsis of bivalents in meiosis. Three out of the 56 patients studied were carriers of microdeletions in the AZF region, one of them also presenting a chromosomal mosaicism for an extra i(22p).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Male infertility; Cytogenetics; Microdeletions; AZF; Meiotic anomalies.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400002

From rumors to genetic isolates
Provedor de dados: Genetics and Molecular Biology
Autores: E. Castilla,Eduardo; Schuler-Faccini,Lavinia.
Here we propose a registration process for population genetic isolates, usually geographic clusters of genetic disorders, based on the systematic search of rumors, defined as any type of account regardless of its reliability. Systematically ascertained rumors are recorded, and validated through a progressive process of pre-established steps. This paper outlines the conceptual basis for this approach and presents the preliminary results from a rumor-based nationwide registry of genetically isolated populations, named CENISO (Censo Nacional de Isolados), operating in Brazil since 2009. During the first four years of its existence (2009-2013), a total of 191 Rumors were registered and validated, resulting in a prevalence rate of one per million inhabitants of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rumors; Disease clustering; Genetic diseases; Population registers; Brazil.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200005

Simulation of population size and genome saturation level for genetic mapping of recombinant inbred lines (RILs)
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Luciano da Costa e; Cruz,Cosme Damião; Moreira,Maurilio Alves; Barros,Everaldo Gonçalves de.
Various population sizes and number of markers have been used to obtain genetic maps. However, the precise number of individuals and markers needed for obtaining reliable maps is not known. We used data simulation to determine the influence of population size, the effect of the degree of marker saturation of the genome, and the number of individuals required for mapping of recombinant inbred lines (RILs). Three genomes with 11 linkage groups were generated with saturation levels of 5, 10 and 20 cM. For each saturation level populations were generated with 50, 100, 154, 200, 300, 500 and 800 individuals with 100 replications for each population size. A total of 2100 populations was generated and mapped. Small marker numbers and small population sizes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recombinant inbred lines; Molecular markers; Number of individuals; Computer simulation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600013

Targeting STAT-3 signaling pathway in cancer for development of novel drugs: Advancements and challenges
Provedor de dados: Genetics and Molecular Biology
Autores: Arshad,Sundas; Naveed,Muhammad; Ullia,Mahad; Javed,Khadija; Butt,Ayesha; Khawar,Masooma; Amjad,Fazeeha.
Abstract Signal transducers and activators of transcription 3 (STAT-3) is a transcription factor that regulates the gene expression of several target genes. These factors are activated by the binding of cytokines and growth factors with STAT-3 specific receptors on cell membrane. Few years ago, STAT-3 was considered an acute phase response element having several cellular functions such as inflammation, cell survival, invasion, metastasis and proliferation, genetic alteration, and angiogenesis. STAT-3 is activated by several types of inflammatory cytokines, carcinogens, viruses, growth factors, and oncogenes. Thus, the STAT3 pathway is a potential target for cancer therapeutics. Abnormal STAT-3 activity in tumor development and cellular transformation can...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: STAT-3; DNA binding domain; Apoptosis; Drug discovery STAT-3 inhibitors.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100101

Association mapping for yield and grain quality traits in rice (Oryza sativa L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Borba,Tereza Cristina de Oliveira; Brondani,Rosana Pereira Vianello; Breseghello,Flávio; Coelho,Alexandre Siqueira Guedes; Mendonça,João Antônio; Rangel,Paulo Hideo Nakano; Brondani,Claudio.
Association analysis was applied to a panel of accessions of Embrapa Rice Core Collection (ERiCC) with 86 SSR and field data from two experiments. A clear subdivision between lowland and upland accessions was apparent, thereby indicating the presence of population structure. Thirty-two accessions with admixed ancestry were identified through structure analysis, these being discarded from association analysis, thus leaving 210 accessions subdivided into two panels. The association of yield and grain-quality traits with SSR was undertaken with a mixed linear model, with markers and subpopulation as fixed factors, and kinship matrix as a random factor. Eight markers from the two appraised panels showed significant association with four different traits,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Association analysis; Core collection; Genetic structure.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300023

Molecular markers in Chinese carps and their interspecific hybrids
Provedor de dados: Genetics and Molecular Biology
Autores: Fonteles,Soraia B.A.; Fernandes,Flora M. de C.; Lopes,Carlos E.; Kulikoski,Roberto; Kulikoski,Jussara M.; Almeida-Toledo,Lurdes F. de.
A sample consisting of 2 Ctenopharyngodon idella (grass carp) females, 2 Aristichthys nobilis (bighead carp) males, and 10 interspecific hybrids was analyzed using a multidisciplinary approach. The 10 hybrids were the only surviving specimens of a crossbreeding experiment. The cytological approach, involving the measurement of the largest axis of the erythrocytes, revealed that the parental species were diploid, whereas, among the hybrids, eight individuals were triploid, and two were diploid. Two of the triploid individuals were analyzed cytogenetically and showed 2n = 3X = 72 chromosomes. Using the biochemical and molecular approaches, it was possible to confirm that one of the C. idella specimens was the true mother of the hybrids. Those data also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SPAR; Carps; Hybrids.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100030

Estimates of DNA strand breakage in bottlenose dolphin (Tursiops truncatus) leukocytes measured with the Comet and DNA diffusion assays
Provedor de dados: Genetics and Molecular Biology
Autores: Díaz,Adriana; Carro,Sandra; Santiago,Livia; Estévez,Juan; Guevara,Celia; Blanco,Miriam; Sánchez,Laima; Sánchez,Liena; López,Nirka; Cruz,Danilo; López,Ronar; Cuetara,Elizabeth B.; Fuentes,Jorge Luis.
The analysis of DNA damage by mean of Comet or single cell gel electrophoresis (SCGE) assay has been commonly used to assess genotoxic impact in aquatic animals being able to detect exposure to low concentrations of contaminants in a wide range of species. The aims of this work were 1) to evaluate the usefulness of the Comet to detect DNA strand breakage in dolphin leukocytes, 2) to use the DNA diffusion assay to determine the amount of DNA strand breakage associated with apoptosis or necrosis, and 3) to determine the proportion of DNA strand breakage that was unrelated to apoptosis and necrosis. Significant intra-individual variation was observed in all of the estimates of DNA damage. DNA strand breakage was overestimated because a considerable amount...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; DNA diffusion assay; DNA strand breakage; Tursiops truncatus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200025

Phylogenetic analysis of 16S mitochondrial DNA data in sloths and anteaters
Provedor de dados: Genetics and Molecular Biology
Autores: Barros,Maria Claudene; Sampaio,Iracilda; Schneider,Horacio.
We sequenced part of the 16S rRNA mitochondrial gene in 17 extant taxa of Pilosa (sloths and anteaters) and used these sequences along with GenBank sequences of both extant and extinct sloths to perform phylogenetic analysis based on parsimony, maximum-likelihood and Bayesian methods. By increasing the taxa density for anteaters and sloths we were able to clarify some points of the Pilosa phylogenetic tree. Our mitochondrial 16S results show Bradypodidae as a monophyletic and robustly supported clade in all the analysis. However, the Pleistocene fossil Mylodon darwinii does not group significantly to either Bradypodidae or Megalonychidae which indicates that trichotomy best represents the relationship between the families Mylodontidae, Bradypodidae and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pilosa; Sloths; Anteaters; Phylogeny; Mitochondrial DNA; RDNA16S.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100002

Drosophila relics hobo and hobo-MITEs transposons as raw material for new regulatory networks
Provedor de dados: Genetics and Molecular Biology
Autores: Loreto,Elgion L.S.; Deprá,Maríndia; Diesel,José F.; Panzera,Yanina; Valente-Gaiesky,Vera Lucia S.
Abstract Hypermutable strains of Drosophila simulans have been studied for 20 years. Several mutants were isolated and characterized, some of which had phenotypes associated with alteration in development; for example, showing ectopic legs with eyes being expressed in place of antennae. The causal agent of this hypermutability is a non-autonomous hobo-related sequence (hoboVA). Around 100 mobilizable copies of this element are present in the D. simulans genome, and these are likely mobilized by the autonomous and canonical hobo element. We have shown that hoboVA has transcription factor binding sites for the developmental genes, hunchback and even-skipped, and that this transposon is expressed in embryos, following the patterns of these genes. We suggest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hobo; Transposable elements; Cis-regulatory sequences; Drosophila.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200198

Fast, non-toxic, and inexpensive n-butanol preparation of recombinant plasmids
Provedor de dados: Genetics and Molecular Biology
Autores: Brieger,Jürgen; Weidt,Eberhard J.; Decker,Jochen.
Various commercial and non-commercial plasmid preparation protocols are currently available. However, the kits are expensive and many of the protocols contain toxic chemicals. Here we present a novel, optimized and, therefore, very advantageous plasmid preparation protocol using n-butanol. The preparation can be performed quickly and no toxic chemicals are used, at overall costs of about one cent per plasmid preparation.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200010

Genetic variation of the bronze locus (MC1R) in turkeys from Southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Corso,Josmael; Hepp,Diego; Ledur,Mônica C.; Peixoto,Jane O.; Fagundes,Nelson J. R.; Freitas,Thales R. O.
Abstract Domestic turkeys present several color phenotypes controlled by at least five genetic loci, but only one of these has been identified precisely: the bronze locus, which turned out to be the melanocortin-1 receptor (MC1R) gene. MC1R variation is important for breeders interested in maintaining or developing different color varieties. In this study, we sequenced most of the MC1R gene from 16 White Holland (the main commercial turkey variety) and 19 pigmented turkeys from southern Brazil with two purposes. The first was to describe the MC1R diversity in White Holland turkeys, which may serve as reservoirs of genetic diversity at this locus. The second was to test whether the traditional color classification used by Brazilian breeders is related to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meleagris gallopavo; White phenotype; Melanocortin-1 receptor; Bronze locus; Plumage color.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100104

In silico differential display of defense-related expressed sequence tags from sugarcane tissues infected with diazotrophic endophytes
Provedor de dados: Genetics and Molecular Biology
Autores: Lambais,Marcio R.
The expression patterns of 277 sugarcane expressed sequence tags (EST)-contigs encoding putative defense-related (DR) proteins were evaluated using the Sugarcane EST database. The DR proteins evaluated included chitinases, beta-1,3-glucanases, phenylalanine ammonia-lyases, chalcone synthases, chalcone isomerases, isoflavone reductases, hydroxyproline-rich glycoproteins, proline-rich glycoproteins, peroxidases, catalases, superoxide dismutases, WRKY-like transcription factors and proteins involved in cell death control. Putative sugarcane WRKY proteins were compared and their phylogenetic relationships determined. A hierarchical clustering approach was used to identify DR ESTs with similar expression profiles in representative cDNA libraries. To identify DR...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100015

Genetic diversity between herds of Alpine and Saanen dairy goats and the naturalized Brazilian Moxotó breed
Provedor de dados: Genetics and Molecular Biology
Autores: Araújo,Adriana Mello de; Guimarães,Simone Eliza Facioni; Machado,Thea Mírian Medeiros; Lopes,Paulo Sávio; Pereira,Carmen Silva; Silva,Francisco Luiz Ribeiro da; Rodrigues,Marcelo Teixeira; Columbiano,Virgínia de Souza; Fonseca,Cleusa Graça da.
Brazilian naturalized goat breeds are adapted to the semiarid conditions prevalent in the Northeast region of the country (which has the largest Brazilian goat heard) and represent an as yet uninvestigated source of genetic diversity. Currently, imported goat breeds are crossed with Brazilian naturalized goat breeds, endangering the genetic potential of the naturalized breeds. We used 11 microsatellite markers to determine the genetic diversity among imported (non-naturalized) dairy Alpine and Saanen goats and naturalized Brazilian Moxotó goats. We genotyped 292 goats from three herds (one private, one from the University of Minas Gerais and the Moxotó conservation herd from Embrapa Caprinos) and found that the general heterozygosity was 0.6952 for Alpine,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; DNA microsatellite; Genetic distance; Genetic diversity; Goats.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100014

Adaptive evolution of the vertebrate skeletal muscle sodium channel
Provedor de dados: Genetics and Molecular Biology
Autores: Lu,Jian; Zheng,Jianzhou; Xu,Qinggang; Chen,Keping; Zhang,Chiyu.
Tetrodotoxin (TTX) is a highly potent neurotoxin that blocks the action potential by selectively binding to voltage-gated sodium channels (Na v). The skeletal muscle Na v (Na v1.4) channels in most pufferfish species and certain North American garter snakes are resistant to TTX, whereas in most mammals they are TTX-sensitive. It still remains unclear as to whether the difference in this sensitivity among the various vertebrate species can be associated with adaptive evolution. In this study, we investigated the adaptive evolution of the vertebrate Na v1.4 channels. By means of the CODEML program of the PAML 4.3 package, the lineages of both garter snakes and pufferfishes were denoted to be under positive selection. The positively selected sites identified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Skeletal muscle voltage-gated Na (Na v1.4) channel; Tetrodotoxin (TTX); Positive selection; Pufferfish; Garter snake.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200026

Transcriptomics analysis of Psidium cattleyanum Sabine (Myrtaceae) unveil potential genes involved in fruit pigmentation
Provedor de dados: Genetics and Molecular Biology
Autores: Vetö,Nicole M.; Guzman,Frank; Kulcheski,Franceli R.; Segatto,Ana Lúcia A.; Lacerda,Maria Eduarda G.; Margis,Rogerio; Turchetto-Zolet,Andreia C.
Abstract Psidium cattleyanum Sabine is an Atlantic Forest native species that presents some populations with red fruits and others with yellow fruits. This variation in fruit pigmentation in this species is an intriguing character that could be related to species evolution but still needs to be further explored. Our goal was to provide genomic information for these morphotypes to understand the molecular mechanisms of differences in fruit colour in this species. In this study, we performed a comparative transcriptome analysis of red and yellow morphotypes of P. cattleyanum, considering two stages of fruit ripening. The transcriptomic analysis performed encompassing leaves, unripe and ripe fruits, in triplicate for each morphotype. The transcriptome...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthocyanin; Atlantic Forest; Carotenoids; Fruit ripening; Myrtaceae.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400803

Genetic relationships between Chinese, Japanese, and Brazilian soybean gene pools revealed by simple sequence repeat (SSR) markers
Provedor de dados: Genetics and Molecular Biology
Autores: Yamanaka,Naoki; Sato,Hiroyuki; Yang,Zhenyu; Xu,Dong He; Catelli,Lizandra Lucy; Binneck,Eliseu; Arias,Carlos Alberto Arrabal; Abdelnoor,Ricardo Vilela; Nepomuceno,Alexandre Lima.
An understanding of the relationship of geographically different soybean gene pools, based on selectively neutral DNA markers would be useful for the selection of divergent parental cultivars for use in breeding. We assessed the relationships of 194 Chinese, 59 Japanese, and 19 Brazilian soybean cultivars (n = 272) using 12 simple sequence repeat (SSR) markers. Quantification Theory III and clustering analyses showed that the Chinese and Japanese cultivars were genetically quite distant to each other but not independent, while Brazilian cultivars were distantly related to the cultivars from the other two countries and formed a cluster that was distant from the other two gene pool clusters. Our results indicated that the Brazilian soybean gene pool is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA marker; Genetic relationship; Genetic resources; Glycine max.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100016

Diversity analysis of Bemisia tabaci biotypes: RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region
Provedor de dados: Genetics and Molecular Biology
Autores: Rabello,Aline R.; Queiroz,Paulo R.; Simões,Kenya C.C.; Hiragi,Cássia O.; Lima,Luzia H.C.; Oliveira,Maria Regina V.; Mehta,Angela.
The Bemisia tabaci complex is formed by approximately 41 biotypes, two of which (B and BR) occur in Brazil. In this work we aimed at obtaining genetic markers to assess the genetic diversity of the different biotypes. In order to do that we analyzed Bemisia tabaci biotypes B, BR, Q and Cassava using molecular techniques including RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region. The analyses revealed a high similarity between the individuals of the B and Q biotypes, which could be distinguished from the BR individuals. A phylogenetic tree based on ITS1 rDNA sequence was constructed. This is the first report of the ITS1 rDNA sequence of Bemisia tuberculata and of the BR biotype of B. tabaci.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Whitefly; ITS rDNA; Genetic diversity; Phylogenetic analysis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300029

Genetic polymorphisms and haplotypes of the organic cation transporter 1 gene (SLC22A1) in the Xhosa population of South Africa
Provedor de dados: Genetics and Molecular Biology
Autores: Jacobs,Clifford; Pearce,Brendon; Du Plessis,Mornè; Hoosain,Nisreen; Benjeddou,Mongi.
Human organic cation transporter 1 is primarily expressed in hepatocytes and mediates the electrogenic transport of various endogenous and exogenous compounds, including clinically important drugs. Genetic polymorphisms in the gene coding for human organic cation transporter 1, SLC22A1, are increasingly being recognized as a possible mechanism explaining the variable response to clinical drugs, which are substrates for this transporter. The genotypic and allelic distributions of 19 nonsynonymous and one intronic SLC22A1 single nucleotide polymorphisms were determined in 148 healthy Xhosa participants from South Africa, using a SNAPshot® multiplex assay. In addition, haplotype structure for SLC22A1 was inferred from the genotypic data. The minor allele...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; Haplotype; Population genetic structure; Genotyping; Genetic variability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300006

Heterochromatin analysis in the fish species Liposarcus anisitsi (siluriformes) and Leporinus elongatus (characiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Artoni,Roberto Ferreira; Molina,Wagner Franco; Bertollo,Luis Antonio Carlos; Galetti Junior,Pedro Manoel.
The chromosomes of two neotropical freshwater fish species, namely Liposarcus anisitsi (Siluriformes, Loricariidae) and Leporinus elongatus (Characiformes, Anostomidae), were investigated by means of C-banding, Ag-NORs, fluorochrome staining and banding by hot saline solution (HSS) treatment, to reveal patterns of heterochromatin differentiation. The karyotype of L. anisitsi is described for the first time. Staining with the GC-specific fluorescent antibiotic mithramycin (MM) revealed bright signals in some C-banded blocks in both species, suggesting that these MM+ heterochromatin contains GC-rich DNA. Banding by denaturation employing HSS, followed by Giemsa staining, yielded corresponding results documenting the thermal stability of GC-rich DNA part of...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100009

Maximization of genetic gain in rubber tree (Hevea) breeding with effective size restriction
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Reginaldo Brito da; Resende,Marcos Deon Vilela de; Araújo,Antônio José de; Gonçalves,Paulo de Souza; Silva,Marcelo de Almeida.
The heritability coefficients and the genetic gains associated with individual, combined and among and within progeny selection, and with multi-effect index selection in long-term rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell.-Arg.] breeding were determined using effective population size (Ne) restriction. Twenty-two half sib progenies were planted at the Jaú Experimental Station, São Paulo State, Brazil, in a complete randomized block design, with five replications and 10 plants per plot. The following traits were assessed when the plants were three years old: number of laticiferous vessel rings (NR), dry rubber production (RP), bark thickness (BT) and stem girth (SG). Significant variability was found among progeny with good chances of...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200035

Identification of SSR and RAPD markers linked to a resistance allele for angular leaf spot in the common bean (Phaseolus vulgaris) line ESAL 550
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Gilvan Ferreira da; Santos,João Bosco dos; Ramalho,Magno Antonio Patto.
The objective of this study was to identify RAPD and SSR markers associated with a resistant allele for angular leaf spot (Phaeoisariopsis griseola) from the line 'ESAL 550', derived from the Andean 'Jalo EEP 558' cultivar, to assist selection of resistant genotypes. The resistant line 'ESAL 550' and the susceptible cultivar 'Carioca MG' were crossed to generate F1 and F2 populations. One hundred and twenty F2:3 families were evaluated. The DNA of the 12 most resistant families was bulked and the same was done with the DNA of the 10 most susceptible, generating two contrasting bulks. One RAPD and one SSR marker was found to be linked in coupling phase to the resistant allele. The SSR marker was amplified by the primer PV-atct001(282C), and its distance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Phaeoisariopsis griseola; DNA markers; Resistance; Bulk segregant analysis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400009

Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica oleracea var botrytis)
Provedor de dados: Genetics and Molecular Biology
Autores: Marino,Celso Luis; Gimenes,Marcos Aparecido; Silva,Norberto da; Lopes,Catalina Romero.
Eighty-one lines of cauliflower (Brassica oleracea var. botrytis) from 12 populations used to produce commercial hybrids in Brazil were screened for polymorphism in the acid phosphatase system, in order to evaluate the usefulness of this marker for the determination of the parental contamination level in hybrid seeds. Little polymorphism was detected in the examined lines, but the system appeared to be very useful for hybrid identification, since the only condition required was polymorphism between the two parental lines. If the analyzed lines were used for hybrid production, 8.4% and 12.3% of the possible crosses would result in hybrids which can be positively identified using the APS-1 and B1 loci, respectively. If only one plant of each homozygous type...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isoenzyme; Polymorphism; Cauliflower.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100015

Effective population size and the genetic consequences of commercial whaling on the humpback whales (Megaptera novaeangliae) from Southwestern Atlantic Ocean
Provedor de dados: Genetics and Molecular Biology
Autores: Cypriano-Souza,Ana Lúcia; da Silva,Tiago Ferraz; Engel,Márcia H.; Bonatto,Sandro L.
Abstract Genotypes of 10 microsatellite loci of 420 humpback whales from the Southwestern Atlantic Ocean population were used to estimate for the first time its contemporary effective (Ne) and census (Nc) population sizes and to test the genetic effect of commercial whaling. The results are in agreement with our previous studies that found high genetic diversity for this breeding population. Using an approximate Bayesian computation approach, the scenario of constant Ne was significantly supported over scenarios with moderate to strong size changes during the commercial whaling period. The previous generation Nc (Ne multiplied by 3.6), which should corresponds to the years between around 1980 and 1990, was estimated between ~2,600 and 6,800 whales (point...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Commercial whaling; Bottleneck; Humpback whale; Microsatellites; Demography.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200253

Doralice Maria Cella (1954-2013)
Provedor de dados: Genetics and Molecular Biology
Autores: Schneider,Marielle Cristina.
Tipo: Info:eu-repo/semantics/other
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200002

Oncogenetics service and the Brazilian public health system: the experience of a reference Cancer Hospital
Provedor de dados: Genetics and Molecular Biology
Autores: Palmero,Edenir I.; Galvão,Henrique C.R.; Fernandes,Gabriela C.; Paula,André E. de; Oliveira,Junea C.; Souza,Cristiano P.; Andrade,Carlos E.; Romagnolo,Luis G.C.; Volc,Sahlua; C. Neto,Maximiliano; Sabato,Cristina; Grasel,Rebeca; Mauad,Edmundo; Reis,Rui M.; Michelli,Rodrigo A.D.
Abstract The identification of families at-risk for hereditary cancer is extremely important due to the prevention potential in those families. However, the number of Brazilian genetic services providing oncogenetic care is extremely low for the continental dimension of the country and its population. Therefore, at-risk patients do not receive appropriate assistance. This report describes the creation, structure and management of a cancer genetics service in a reference center for cancer prevention and treatment, the Barretos Cancer Hospital (BCH). The Oncogenetics Department (OD) of BCH offers, free of charge, to all patients/relatives with clinical criteria, the possibility to perform i) genetic counseling, ii) preventive examinations and iii) genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oncogenetics; Hereditary cancer; Public health.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200168

Molecular evolution of the ent-kaurenoic acid oxidase gene in Oryzeae
Provedor de dados: Genetics and Molecular Biology
Autores: Yang,Yanhua; Chen,Keping.
We surveyed the substitution patterns in the ent-kaurenoic acid oxidase (KAO) gene in 11 species of Oryzeae with an outgroup in the Ehrhartoidaea. The synonymous and non-synonymous substitution rates showed a high positive correlation with each other, but were negatively correlated with codon usage bias and GC content at third codon positions. The substitution rate was heterogenous among lineages. Likelihood-ratio tests showed that the non-synonymous/synonymous rate ratio changed significantly among lineages. Site-specific models provided no evidence for positive selection of particular amino acid sites in any codon of the KAO gene. This finding suggested that the significant rate heterogeneity among some lineages may have been caused by variability in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Codon usage bias; Ent-kaurenoic acid oxidase (KAO); Positive selection; Rate heterogeneity; Substitution rate.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300011

X1X1X2X2/X1X2Y sex chromosome systems in the Neotropical Gymnotiformes electric fish of the genus Brachyhypopomus
Provedor de dados: Genetics and Molecular Biology
Autores: Cardoso,Adauto Lima; Pieczarka,Julio Cesar; Nagamachi,Cleusa Yoshiko.
Several types of sex chromosome systems have been recorded among Gymnotiformes, including male and female heterogamety, simple and multiple sex chromosomes, and different mechanisms of origin and evolution. The X1X1X2X2/X1X2Y systems identified in three species of this order are considered homoplasic for the group. In the genus Brachyhypopomus, only B. gauderio presented this type of system. Herein we describe the karyotypes of Brachyhypopomus pinnicaudatus and B. n. sp. FLAV, which have an X1X1X2X2/X1X2Y sex chromosome system that evolved via fusion between an autosome and the Y chromosome. The morphology of the chromosomes and the meiotic pairing suggest that the sex chromosomes of B. gauderio and B. pinnicaudatus have a common origin, whereas in B . n....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sex chromosome fusion; Sexual trivalent; Meiosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200213

Genetic variability in Brazilian wheat cultivars assessed by microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Schuster,Ivan; Vieira,Elisa Serra Negra; Silva,Glacy Jaqueline da; Franco,Francisco de Assis; Marchioro,Volmir Sérgio.
Wheat (Triticum aestivum) is one of the most important food staples in the south of Brazil. Understanding genetic variability among the assortment of Brazilian wheat is important for breeding. The aim of this work was to molecularly characterize the thirty-six wheat cultivars recommended for various regions of Brazil, and to assess mutual genetic distances, through the use of microsatellite markers. Twenty three polymorphic microsatellite markers (PMM) delineated all 36 of the samples, revealing a total of 74 simple sequence repeat (SSR) alleles, i.e. an average of 3.2 alleles per locus. Polymorphic information content (PIC value) calculated to assess the informativeness of each marker ranged from 0.20 to 0.79, with a mean of 0.49. Genetic distances among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Germplasm; Cultivar characterization; Cluster analysis; Molecular markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300019

Dissecting the sugarcane expressed sequence tag (SUCEST) database: unraveling flower-specific genes
Provedor de dados: Genetics and Molecular Biology
Autores: Figueiredo,R.C.; Brito,M.S.; Figueiredo,L.H.M.; Quiapin,A.C.; Vitorelli,P.M.; Silva,L.R.; Santos,R.V.; Molfetta,J.B.; Goldman,G.H.; Goldman,M.H.S.
There are almost 260,000 independent clones sequenced from the 5’ end in the Sugarcane Expressed Sequence Tag (SUCEST) database, which have been obtained from 37 cDNA libraries prepared from different tissues. This large number of expressed sequence tags (ESTs) provides an opportunity, unprecedented in plants, to perform ‘digital differential screening’ on selected cDNA libraries. In general, the frequency of a particular EST correlates with transcript accumulation in the tissues from which the cDNA libraries were constructed, so it is possible to compare the whole transcriptome from different tissues using computer-assisted analysis of an EST database. In our research we analyzed sugarcane ESTs according to tissue expression and identified more than 1,000...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100012

Growth hormone insensitivity with immune dysfunction caused by a STAT5B mutation in the south of Brazil: evidence for a founder effect
Provedor de dados: Genetics and Molecular Biology
Autores: Scalco,Renata C.; Gonçalves,Fernanda T.; Santos,Hadassa C.; Cardena,Mari M. S. G.; Tonelli,Carlos A.; Funari,Mariana F. A.; Aracava,Rosana M.; Pereira,Alexandre C.; Fridman,Cintia; Jorge,Alexander A. L.
ABSTRACT Homozygous STAT5B mutations causing growth hormone insensitivity with immune dysfunction were described in 10 patients since 2003, including two Brazilian brothers from the south of Brazil. Our objectives were to evaluate the prevalence of their STAT5B mutation in this region and to analyze the presence of a founder effect. We obtained DNA samples from 1,205 local inhabitants, 48 relatives of the homozygous patients and four individuals of another affected family. Genotyping for STAT5B c.424_427del mutation and for two polymorphic markers around it was done through fragment analysis technique. We also determined Y-chromosome and mtDNA haplotypes and genomic ancestry in heterozygous carriers. We identified seven families with STAT5B c.424_427del...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Founder effect; Growth hormone insensitivity; Immune dysfunction; STAT5B.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300436

Mannose-Binding Lectin 2 (MBL2) combined genotypes deficiency is associated with susceptibility for Oral Lichen Planus
Provedor de dados: Genetics and Molecular Biology
Autores: Polesello,Vania; Segat,Ludovica; Biasotto,Matteo; Ottaviani,Giulia; Gobbo,Margherita; Di Lenarda,Roberto; Crovella,Sergio; Zupin,Luisa.
Abstract Oral Lichen Planus (OLP) is an oral inflammatory condition, mediated by host immune system reaction, presenting basal membrane damages with inflammatory lesions in the mouth and/or skin. In this study, the role of functional polymorphisms in the MBL2 gene, encoding for Mannose-Binding Protein C (MBP-C), a member of the innate immune response and an acute-phase protein able to activate the complement cascade, was investigated to assess a possible association with OLP susceptibility in Italian patients. Two variations at the promoter region (called H/L and X/Y) and three at the first exon (at codon 52, 54, and 57) of the MBL2 gene were analyzed in 69 OLP patients and 244 healthy controls from northeastern Italy. Considering the polymorphisms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mannose-Binding Protein-C; MBL2; Oral Lichen Planus; Polymorphisms.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100009

Association between osteoprotegerin gene polymorphisms and cardiovascular disease in type 2 diabetic patients
Provedor de dados: Genetics and Molecular Biology
Autores: Guo,Changlei; Hu,Fudong; Zhang,Shaoli; Wang,Yakun; Liu,Hengdao.
Osteoprotegerin (OPG) gene polymorphisms (T245G, T950C and G1181C) have been associated with osteoporosis and early predictors of cardiovascular disease. The aim of this study was to evaluate whether these polymorphisms contribute to cardiovascular disease (CVD) in type 2 diabetic patients. We performed a case-control study with 178 CVD subjects with diabetes and 312 diabetic patients without CVD to assess the impact of variants of the OPG gene on the risk of CVD. The OPG gene polymorphisms were analyzed by using the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). There was no significant association between the T245G and G1181C polymorphisms and CVD in the additive genetic model (OR = 0.96, 95% CI 0.64-1.45, p = 0.79;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular disease; Osteoprotegerin; Polymorphism.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200006

Bioinformatics for the Citrus EST Project (CitEST)
Provedor de dados: Genetics and Molecular Biology
Autores: Reis,Marcelo S.; Takita,Marco A.; Palmieri,Darío A.; Machado,Marcos A.
In this work we describe all the computational environments, pipelines, and web services developed for the CitEST transcriptome project, on which all the annotation researchers relied. We also present a complete list of CitEST libraries and, for each of them, the general features after the in silico processing, showing some quantitative information.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transcriptomics; Comparative genomics; Clustering; Trimming; Digital northern.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500031

Systematic molecular analysis of hemophilia A patients from Colombia
Provedor de dados: Genetics and Molecular Biology
Autores: Yunis,Luz Karime; Linares,Adriana; Cabrera,Edgar; Yunis,Juan J.
Abstract Hemophilia A (HA) is an X-linked recessive disorder and the second most common coagulation disorder with an incidence of 1 in 5,000 live born males. Worldwide, there are 178,500 affected individuals, 60% with the severe form of the disease. Intron 22 and 1 inversions (Inv22 and Inv1) are the most frequent molecular alterations found in severe HA patients with a frequency of 45-50% and 0.5-5%, respectively. We have implemented a systematic cost-effective strategy for the identification of the molecular alteration in HA patients using Inverse shifting-PCR for Inv22 and Inv1, followed by the analysis of the F8 gene coding region by means of high resolution melting (HRM) PCR and Sanger sequencing in Inv22 and Inv1 negative patients. A total of 33 male...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemophilia A; Factor VIII; IS-PCR; HRM; Colombia.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500750

Molecular studies of Callithrix pygmaea (Primates, Platyrrhini) based on transferrin intronic and ND1 regions: implications for taxonomy and conservation
Provedor de dados: Genetics and Molecular Biology
Autores: Tagliaro,Claudia Helena; Schneider,Maria Paula Cruz; Schneider,Horacio; Sampaio,Iracilda; Stanhope,Michael.
Traditional classifications of Platyrrhini monkeys, based mainly on morphological features, are being contested by recent molecular data. The subfamily Callitrichinae (Platyrrhini, Primates) consists of a diverse group of species, many of them considered endangered. Our analysis of two DNA regions, a mtDNA gene (ND1) and a nuclear gene (intronic regions of the transferrin gene), suggests that Callithrix pygmaea may have sufficient variability to justify the existence of subspecies or even separate species. Phylogenetic dendrograms based on the ND1 region show that this species is more closely related to Amazonian than to Atlantic forest marmosets. These results reopen the discussion about diversity and conservation programs based exclusively on traditional...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400006

Selection for breed-specific growth hormone and IGF-I alleles in a synthetic beef cattle cross, Canchim
Provedor de dados: Genetics and Molecular Biology
Autores: Regitano,L.C.A.; Azevedo,J.L.; Vencovsky,R.; Packer,I.U.; Barbosa,P.F.; Rosa,A.J.M.; Silva,N.A.; Etchegaray,M.A.L.; Coutinho,L.L.
This study was developed to evaluate selection effects on gene frequencies in a synthetic beef cattle cross (5/8 Charolais, 3/8 Zebu) named Canchim. A sample of 154 Canchim animals, representing three generation classes, was analyzed for seven molecular markers. Thirty-six Charolais cattle were also studied for comparison. A highly significant variation (P &amp;lt; 0.01), with a linear increase of the allele coding for valine at position 127 of the growth hormone peptide, was observed through generations. This allele was found in the Charolais sample but is not observed in Brazilian Bos indicus breeds. Four alleles were found for a microsatellite in the 5' flanking region of insulin-like growth factor I gene in Canchim. Allele sizes ranged from 231 to 225...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400011

Comparative analysis of the genes UL1 through UL7 of the duck enteritis virus and other herpesviruses of the subfamily  Alphaherpesvirinae
Provedor de dados: Genetics and Molecular Biology
Autores: Li,Huixin; Liu,Shengwang; Han,Zongxi; Shao,Yuhao; Chen,Shuhong; Kong,Xiangang.
The nucleotide sequences of eight open reading frames (ORFs) located at the 5' end of the unique long region of the duck enteritis virus (DEV) Clone-03 strain were determined. The genes identified were designated  UL1 ,  UL2 ,  UL3 ,  UL4 ,  UL5 ,  UL6  and  UL 7 homologues of the herpes simplex virus 1 (HSV-1). The DEV  UL3.5  located between  UL3  and  UL4  had no homologue in the HSV-1. The arrangement and transcription orientation of the eight genes were collinear with their homologues in the HSV-1. Phylogenetic trees were constructed based on the alignments of the deduced amino acids of eight proteins with their homologues in 12 alpha-herpesviruses. In the UL1, UL3, UL3.5, UL5 and UL7 proteins trees, the branches were more closely related to the genus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Duck enteritis virus; UL1-UL7 genes; Phylogenetic analysis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100018

Genetic variability and selection for laticiferous system characters in Hevea brasiliensis
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Paulo de Souza; Cardinal,Átila Bento Beleti; Costa,Reginaldo Brito da; Bortoletto,Nelson; Gouvêa,Lígia Regina Lima.
Six laticiferous system characters were investigated in 22 three-year-old, half-sib rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell.-Arg.] progenies, evaluated at three sites (Votuporanga, Pindorama and Jaú, all in the São Paulo State, Brazil). The traits examined were: average rubber yield (Pp), average bark thickness (Bt), number of latex vessel rings (Lv), average distance between consecutive latex vessel rings (Dc), density of latex vessels per 5 mm per ring averaged over all rings (Dd) and the diameter of the latex vessels (Di). The joint analysis showed that site effect and progeny x sites interaction were significant for all traits, except Lv. Estimates of individual heritabilities across the three sites were high for Bt; moderate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hevea brasiliensis; Rubber yield; Laticiferous traits; Genetic parameters.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300014

Clustering of water bodies in unpolluted and polluted environments based on Escherichia coli phylogroup abundance using a simple interaction database
Provedor de dados: Genetics and Molecular Biology
Autores: Stoppe,Nancy de Castro; Silva,Juliana Saragiotto; Torres,Tatiana Teixeira; Carlos,Camila; Hachich,Elayse Maria; Sato,Maria Inês Zanoli; Saraiva,Antonio Mauro; Ottoboni,Laura Maria Mariscal.
Different types of water bodies, including lakes, streams, and coastal marine waters, are often susceptible to fecal contamination from a range of point and nonpoint sources, and have been evaluated using fecal indicator microorganisms. The most commonly used fecal indicator is Escherichia coli, but traditional cultivation methods do not allow discrimination of the source of pollution. The use of triplex PCR offers an approach that is fast and inexpensive, and here enabled the identification of phylogroups. The phylogenetic distribution of E. coli subgroups isolated from water samples revealed higher frequencies of subgroups A1 and B2(3) in rivers impacted by human pollution sources, while subgroups D1 and D2 were associated with pristine sites, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: E. coli; Phylogenetic groups; Pollution sources; Interaction networks; Social network analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500012

An investigation of the nuclei of hülle cells of Aspergillus nidulans
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Marcos D.F.; Baracho,Marta S.; Baracho,Ivanhoé R.
Certain species of fungi of the genus Aspergillus produce a type of cells around the cleistothecium that are called hülle cells. To facilitate the analysis of the formation of these cells, an Aspergillus nidulans strain with high hülle cell production was obtained. Hülle cells from this strain were allowed to grow on dialysis membranes, where it was possible to observe their nuclei. The material was fixed with Carnoy, hydrolyzed with HCl and stained with Giemsa. Several nuclei approximately equal in size to the nuclei of hyphae and conidia were observed during the phase of hülle cell formation. The formation of a macronucleus measuring approximately 3.1 mum was observed in mature hülle cells. To compare the DNA amount, hülle cells and conidia of the CH-89...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus nidulans; Hülle cell; Macronuclei in fungi; Nuclei of A. nidulans; Macronuclei in hülle cell.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400019

A rare case of deafness and renal abnormalities in HDR syndrome caused by a de novo mutation in the GATA3 gene
Provedor de dados: Genetics and Molecular Biology
Autores: Martins,Fábio Tadeu Arrojo; Ramos,Berenice Dias; Sartorato,Edi Lúcia.
Abstract HDR syndrome is a rare autosomal dominant disorder caused by mutations in the GATA3 gene and characterized by hypoparathyroidism, sensorineural deafness and renal abnormalities. Here we report a Brazilian family, from which the proband, his mother and his grandfather were diagnosed with bilateral sensorineural hearing loss. Molecular screening of the GJB2, GJB6 and MTRNR1 genes in the proband showed no alterations; however, whole exome sequencing detected a heterozygous mutation, c.1099C &gt; T (p.Arg367*), in the GATA3 gene. Segregation analyses showed that the mother also had the mutation, but not the grandparents, hence indicating a different hearing impairment type for the grandfather. Paternity test of the mother of the proband confirmed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HDR syndrome; Hypoparathyroidism; Deafness; Renal abnormalities; Whole exome sequencing.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500794

Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Nogueira,Lídia; Zanoni,Juliani Bruna; Solé,Mirco; Affonso,Paulo Roberto Antunes de Mello; Siqueira,Sérgio; Sampaio,Iracilda.
Scinax species are still underrepresented in cytogenetic studies, mainly with respect to populations from northeastern and northern Brazil. In this study, we provide new chromosomal information on Scinax boesemani, S. camposseabrai, S. garbei, S. pachycrus, S. trilineatus and S. x-signatus, all belonging to clade S. ruber. They were collected at two locations in the Caatinga biome (northeastern Brazil) and at one in the Amazon (northern Brazil) biomes. Chromosomes were analyzed by conventional staining, C-banding, Ag-NOR staining, and fluorochrome staining. All species shared a modal diploid value of 2n = 24 and fundamental arm number (FN) of 48. Moreover, both chromosomal size and morphology were similar to other species in this Scinax clade. C-banding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphibians; Chromosomes; Fluorochromes; Heterochromatin; RDNA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200156

Environmental and genetic factors affecting mutability to aminoglycoside antibiotics among Escherichia coli K12 strains
Provedor de dados: Genetics and Molecular Biology
Autores: Monteiro,A.C.M.; Ferreira,R.C.C; Padilla,G.; Ferreira,L.C.S.; Costa,S.O.P.
Environmental and genetic factors affecting the in vitro spontaneous mutation frequencies to aminoglycoside resistance in Escherichia coli K12 were investigated. Spontaneous mutation frequencies to kanamycin resistance were at least 100 fold higher on modified Luria agar (L2) plates, when compared to results obtained in experiments carried out with Nutrient agar (NA) plates. In contrast to rifampincin, the increased mutability to kanamycin resistance could not be attributed to a mutator phenotype expressed by DNA repair defective strains. Kanamycin mutant selection windows and mutant preventive concentrations on L2 plates were at least fourfold higher than on NA plates, further demonstrating the role of growth medium composition on the mutability to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic resistance; Aminoglycosides; Escherichia coli K12; Mutator strains; Spontaneous mutantion frequencies.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200017

Identification and characterization of two critical sequences in SV40PolyA that activate the green fluorescent protein reporter gene
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,Honggang; Sun,Wuzhuang; Li,Zhu; Wang,Xiufang; Lv,Zhanjun.
Alu repeats or Line-1-ORF2 (ORF2) inhibit expression of the green fluorescent protein (GFP) gene when inserted downstream of this gene in the vector pEGFP-C1. In this work, we studied cis-acting elements that eliminated the repression of GFP gene expression induced by Alu and ORF2 and sequence characteristics of these elements. We found that sense and antisense PolyA of simian virus 40 (SV40PolyA, 240 bp) eliminated the repression of GFP gene expression when inserted between the GFP gene and the Alu (283 bp) repeats or ORF2 (3825 bp) in pAlu14 (14 tandem Alu repeats were inserted downstream of the GFP gene in the vector pEGFP-C1) or pORF2. Antisense SV40PolyA (PolyAas) induced stronger gene expression than its sense orientation (PolyA). Of four 60-bp...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alu; Enhancer; GFP; Stem-loop structures; SV40PolyA.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300005

Neural restrictive silencer factor and choline acetyltransferase expression in cerebral tissue of Alzheimer's Disease patients: a pilot study
Provedor de dados: Genetics and Molecular Biology
Autores: González-Castañeda,Rocío E.; Sánchez-González,Víctor J.; Flores-Soto,Mario; Vázquez-Camacho,Gonzalo; Macías-Islas,Miguel A.; Ortiz,Genaro G.
Decreased Choline Acetyltransferase (ChAT) brain level is one of the main biochemical disorders in Alzheimer's Disease (AD). In rodents, recent data show that the CHAT gene can be regulated by a neural restrictive silencer factor (NRSF). The aim of the present work was to evaluate the gene and protein expression of CHAT and NRSF in frontal, temporal, entorhinal and parietal cortices of AD patient brains. Four brains from patients with AD and four brains from subjects without dementia were studied. Cerebral tissues were obtained and processed by the guanidine isothiocyanate method for RNA extraction. CHAT and NRSF gene and protein expression were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. CHAT gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neural restrictive silencer factor; Choline acetyltransferase; Alzheimer's disease; Protein expression; Brain.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100005

Lapa vermelha IV Hominid 1: morphological affinities of the earliest known American
Provedor de dados: Genetics and Molecular Biology
Autores: Neves,Walter A.; Powell,Joseph F.; Prous,Andre; Ozolins,Erik G.; Blum,Max.
Several studies concerning the extra-continental morphological affinities of Paleo-Indian skeletons, carried out independently in South and North America, have indicated that the Americas were first occupied by non-Mongoloids that made their way to the New World through the Bering Strait in ancient times. The first South Americans show a clear resemblance to modern South Pacific and African populations, while the first North Americans seem to be at an unresolved morphological position between modern South Pacific and Europeans. In none of these analyses the first Americans show any resemblance to either northeast Asians or modern native Americans. So far, these studies have included affirmed and putative early skeletons thought to date between 8,000 and...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400001

Genotoxic potential of the latex from cotton-leaf physicnut (Jatropha gossypiifolia L.)
Provedor de dados: Genetics and Molecular Biology
Autores: de Almeida,Pedro Marcos; de Sousa Araújo,Silvany; Marin-Morales,Maria Aparecida; Benko-Iseppon,Ana Maria; Brasileiro-Vidal,Ana Christina.
Jatropha gossypiifolia L. (Euphorbiaceae), popularly known as cotton-leaf physicnut, is a milky shrub notable for its medicinal properties. The present study aimed to evaluate the toxic, cytotoxic and genotoxic effects of the latex of J. gossypiifolia, using Allium cepa L. as test system. Seeds of A. cepa were exposed to five concentrations of the latex (1.25; 2.5; 5; 10 and 20 mL/L) in order to evaluate parameters of toxicity (evaluation of root growth), cytotoxicity (mitotic index frequency) and genotoxicity (frequency of chromosome alterations). The latex showed a significant decrease in root mean growth value as well as mitotic index for the tested concentrations, except for 1.25 mL/L, when compared to results from the negative control. The 1.25, 2.5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa test; Medicinal plant; Plant latex; Genotoxicity evaluation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100093

Valproic acid inhibits the invasion of PC3 prostate cancer cells by upregulating the metastasis suppressor protein NDRG1
Provedor de dados: Genetics and Molecular Biology
Autores: Lee,Jae Eun; Kim,Jung Hwa.
Abstract Valproic acid (VPA) is a clinically available histone deacetylase inhibitor with promising anticancer attributes. Recent studies have demonstrated the anticancer effects of VPA on prostate cancer cells. However, little is known about the differential effects of VPA between metastatic and non-metastatic prostate cancer cells and the relationship between the expression of metastasis suppressor proteins and VPA. In the present study, we demonstrate that inhibition of cell viability and invasion by VPA was more effective in the metastatic prostate cancer cell line PC3 than in the tumorigenic but non-metastatic prostate cell line, RWPE2. Further, we identified that the metastasis suppressor NDRG1 is upregulated in PC3 by VPA treatment. In contrast,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: VPA; NDRG1; Prostate cancer cells.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400527

Development of microsatellite markers for identifying Brazilian Coffea arabica varieties
Provedor de dados: Genetics and Molecular Biology
Autores: Vieira,Elisa S.N.; Von Pinho,Édila V. de R.; Carvalho,Maria G.G.; Esselink,Danny G.; Vosman,Ben.
Microsatellite markers, also known as SSRs (Simple Sequence Repeats), have proved to be excellent tools for identifying variety and determining genetic relationships. A set of 127 SSR markers was used to analyze genetic similarity in twenty five Coffea arabica varieties. These were composed of nineteen commercially important Brazilians and six interspecific hybrids of Coffea arabica, Coffea canephora and Coffea liberica. The set used comprised 52 newly developed SSR markers derived from microsatellite enriched libraries, 56 designed on the basis of coffee SSR sequences available from public databases, 6 already published, and 13 universal chloroplast microsatellite markers. Only 22 were polymorphic, these detecting 2-7 alleles per marker, an average of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; Coffee; Genetic similarity; Molecular marker.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300022

Co-transformation of a tropical maize endophytic isolate of Fusarium verticillioides (synonym F. moniliforme) with gusA and nia genes
Provedor de dados: Genetics and Molecular Biology
Autores: Pamphile,João A.; Rocha,Carmen Lúcia M.S.C. da; Azevedo,João L.
A tropical endophytic isolate of the fungus Fusarium verticillioides (synonym Fusarium moniliforme) obtained from Zea mays was co-transformed with plasmid pNH24 containing the Fusarium oxysporum nitrate reductase nia gene and plasmid pNOM 102 carrying the Escherichia coli b-glucuronidase gusA gene. Transformation frequency for the nia marker was 75 transformants mg-1 vector DNA and introduction of the gusA gene by co-transformation was 57.2% as indicated by the presence of the GUS+ phenotype on plates. Southern analyses confirmed the integration of both plasmids into the genome of ten GUS+ transformants. All co-transformants showed mitotic stability in respect of the GUS+ phenotype. To assess the potential of transformed endophytic fungi as vectors for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Co-transformation; Gus; Nia; Fusarium verticillioides; Endophyte.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200021

Signaling pathways in a Citrus EST database
Provedor de dados: Genetics and Molecular Biology
Autores: Mehta,Angela; Silva,Marilia Santos; Guidetti-Gonzalez,Simone; Carrer,Helaine; Takita,Marco Aurélio; Martins,Natália F.
Citrus spp. are economically important crops, which in Brazil are grown mainly in the State of São Paulo. Citrus cultures are attacked by several pathogens, causing severe yield losses. In order to better understand this culture, the Millenium Project (IAC Cordeirópolis) was launched in order to sequence Citrus ESTs (expressed sequence tags) from different tissues, including leaf, bark, fruit, root and flower. Plants were submitted to biotic and abiotic stresses and investigated under different development stages (adult vs. juvenile). Several cDNA libraries were constructed and the sequences obtained formed the Citrus ESTs database with almost 200,000 sequences. Searches were performed in the Citrus database to investigate the presence of different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Cell signaling; Genomics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500003

Evaluation of the defensive behavior of two honeybee ecotypes using a laboratory test
Provedor de dados: Genetics and Molecular Biology
Autores: Andere,Cecilia; Palacio,M.A.; Rodriguez,E.M.; Figini,E.; Dominguez,M.T.; Bedascarrasbure,E.
Honeybee defensive behavior is a useful selection criterion, especially in areas with Africanized honeybees (Apis mellifera L). In all genetic improvement programs the selected characters must be measured with precision, and because of this we evaluated a metabolic method for testing honeybee defensive behavior in the laboratory for its usefulness in distinguishing between honeybee ecotypes and selecting honeybees based on their level of defensive responses. Ten honeybee colonies were used, five having been produced by feral queens from a subtropical region supposedly colonized by Africanized honeybees and five by queens from a temperate region apparently colonized by European honeybees. We evaluate honeybee defensive behavior using a metabolic test based...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Honeybee; Defensive behavior; Oxygen consumption.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100011

A test of the utility of DNA barcoding in the radiation of the freshwater stingray genus Potamotrygon (Potamotrygonidae, Myliobatiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Toffoli,Daniel; Hrbek,Tomas; Araújo,Maria Lúcia Góes de; Almeida,Maurício Pinto de; Charvet-Almeida,Patricia; Farias,Izeni Pires.
DNA barcoding is a recently proposed global standard in taxonomy based on DNA sequences. The two main goals of DNA barcoding methodology are assignment of specimens to a species and discovery of new species. There are two main underlying assumptions: i) reciprocal monophyly of species, and ii) intraspecific divergence is always less than interspecific divergence. Here we present a phylogenetic analysis of the family Potamotrygonidae based on mitochondrial cytochrome c oxidase I gene, sampling 10 out of the 18 to 20 valid species including two non-described species. Potamotrygonidae systematics is still not fully resolved with several still-to-be-described species while some other species are difficult to delimit due to overlap in morphological characters...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potamotrygon; Barcoding; Radiation; Phylogenetics; COI; MtDNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200028

The genomes of three Bradyrhizobium sp. isolated from root nodules of Lupinus albescens grown in extremely poor soils display important genes for resistance to environmental stress
Provedor de dados: Genetics and Molecular Biology
Autores: Granada,Camille E.; Vargas,Luciano K.; Sant’Anna,Fernando Hayashi; Balsanelli,Eduardo; Baura,Valter Antonio de; Oliveira Pedrosa,Fábio de; Souza,Emanuel Maltempi de; Falcon,Tiago; Passaglia,Luciane M.P.
Abstract Lupinus albescens is a resistant cover plant that establishes symbiotic relationships with bacteria belonging to the Bradyrhizobium genus. This symbiosis helps the development of these plants in adverse environmental conditions, such as the ones found in arenized areas of Southern Brazil. This work studied three Bradyrhizobium sp. (AS23, NAS80 and NAS96) isolated from L. albescens plants that grow in extremely poor soils (arenized areas and adjacent grasslands). The genomes of these three strains were sequenced in the Ion Torrent platform using the IonXpress library preparation kit, and presented a total number of bases of 1,230,460,823 for AS23, 1,320,104,022 for NAS80, and 1,236,105,093 for NAS96. The genome comparison with closest strains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bradyrhizobium sp.; Arenized areas; Poor soils; Resistant bacteria.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300502

Genetic diversity and DNA content of three South American and three Eurasiatic Trifolium species
Provedor de dados: Genetics and Molecular Biology
Autores: Rizza,Marco Dalla; Real,Daniel; Reyno,Rafael; Porro,Valentina; Burgueño,Juan; Errico,Eugenia; Quesenberry,Kenneth H.
Six species of Trifolium (T. polymorphum Poir., T. riograndense Burkart, T. argentinense Speg., T. medium L., T. pratense L. and T. repens L.) were analyzed using inter-simple sequence repeats (ISSR) markers. Six selected primers generated 186 polymerase chain reaction (PCR) products exploring 112 loci in 34 genotypes analyzed with molecular sizes ranging from 200 to 1300 bp. These primers were able to discriminate among and within species, with the PCR products being on average 41.6% species-specific and 59.9% polymorphic at the within species level. Nuclear DNA content was determined by flow cytometry and revealed variation among species. The 1Cx genome size values were calculated and were found to range from 0.46 pg (T. pratense) to 0.96 pg (T....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA content; Genetic variability; Inter-SSR; Trifolium spp.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600015

Repetitive DNAs and shrink genomes: A chromosomal analysis in nine Columbidae species (Aves, Columbiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Kretschmer,Rafael; de Oliveira,Thays Duarte; de Oliveira Furo,Ivanete; Oliveira Silva,Fabio Augusto; Gunski,Ricardo José; del Valle Garnero,Analía; de Bello Cioffi,Marcelo; de Oliveira,Edivaldo Herculano Corrêa; de Freitas,Thales Renato Ochotorena.
Abstract An extensive karyotype variation is found among species belonging to the Columbidae family of birds (Columbiformes), both in diploid number and chromosomal morphology. Although clusters of repetitive DNA sequences play an important role in chromosomal instability, and therefore in chromosomal rearrangements, little is known about their distribution and amount in avian genomes. The aim of this study was to analyze the distribution of 11 distinct microsatellite sequences, as well as clusters of 18S rDNA, in nine different Columbidae species, correlating their distribution with the occurrence of chromosomal rearrangements. We found 2n values ranging from 76 to 86 and nine out of 11 microsatellite sequences showed distinct hybridization signals among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birds; FISH; Microsatellites; Sex chromosomes; Chromosomal rearrangements.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100098

Use of chromosome microdissection in fish molecular cytogenetics
Provedor de dados: Genetics and Molecular Biology
Autores: Henning,Frederico; Trifonov,Vladimir; Almeida-Toledo,Lurdes Foresti de.
Chromosome microdissection is a technique in which whole chromosomes or chromosomal segments are dissected under an inverted microscope yielding chromosome-specific sequences. Several protocol modifications introduced during the past 15 years reduced the number of chromosomes required for most applications. This is of particular interest to fish molecular cytogenetics, since most species present highly uniform karyotypes which make impossible the collection of multiple copies of the same chromosome. Probes developed in this manner can be used to investigate chromosome homologies in closely related species. Here we describe a protocol recently used in the gymnotiform species group Eigenmannia and review the major steps involved in the generation of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluorescence in situ hybridization; Chromosome painting; Sex chromosomes; Cross-FISH.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200022

5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers
Provedor de dados: Genetics and Molecular Biology
Autores: Alves-Costa,Fernanda A.; Martins,Cesar; Matos,Fernanda Del Campos de; Foresti,Fausto; Oliveira,Claudio; Wasko,Adriane P.
In order to extend the genetic data on the Sciaenidae fish family, the present study had the purpose to characterize PCR-generated 5S rDNA repeats of twelve species of this group through PAGE (Polyacrylamide Gel Electrophoresis) analysis. The results showed the occurrence of at least two different 5S rDNA size classes in all the species. Moreover, 5S rDNA repeats of one of the studied species - Isopisthus parvipinnis - were cloned and subjected to nucleotide sequencing and Southern blot membrane hybridization analyses, which permitted to confirm the existence of two major 5S rDNA classes. Phylogenetic analysis based on the nucleotide sequences of different 5S rDNA repeats of I. parvipinnis lead to their separation into two major clusters. These results may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sciaenidae; Fish; Isopisthus parvipinnis; 5S rDNA; Molecular markers.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200025

A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction
Provedor de dados: Genetics and Molecular Biology
Autores: Santelli,Roberto V.; Navarro-Cattapan,Luci Deise.
A modification of the CsCl gradient centrifugation method for DNA phage purification is presented. It avoids the enzymatic steps as well the need for a preliminary phage titration, a tedious process proposed in the majority of the protocols in use. The quality of the DNA obtained makes it amenable for additional manipulations like digestions, ligations, labelling, subcloning, etc.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100011

Effects of L1-ORF2 fragments on green fluorescent protein gene expression
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,Xiu-Fang; Jin,Xia; Wang,Xiaoyan; Liu,Jing; Feng,Jingjing; Yang,QinQing; Mu,Wenli; Shi,Xiaojuan; Lu,Zhanjun.
The retrotransposon known as long interspersed nuclear element-1 (L1) is 6 kb long, although most L1s in mammalian and other eukaryotic cells are truncated. L1 contains two open reading frames, ORF1 and ORF2, that code for an RNA-binding protein and a protein with endonuclease and reverse transcriptase activities, respectively. In this work, we examined the effects of full length L1-ORF2 and ORF2 fragments on green fluorescent protein gene (GFP) expression when inserted into the pEGFP-C1 vector downstream of GFP. All of the ORF2 fragments in sense orientation inhibited GFP expression more than when in antisense orientation, which suggests that small ORF2 fragments contribute to the distinct inhibitory effects of this ORF on gene expression. These results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; Green fluorescent protein gene; L1-ORF2; Transcription termination; Orientation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400003

Comparative genomics of an endophytic Pseudomonas putida isolated from mango orchard
Provedor de dados: Genetics and Molecular Biology
Autores: Asif,Huma; Studholme,David J.; Khan,Asifullah; Aurongzeb,M.; Khan,Ishtiaq A.; Azim,M. Kamran.
Abstract We analyzed the genome sequence of an endophytic bacterial strain Pseudomonas putida TJI51 isolated from mango bark tissues. Next generation DNA sequencing and short read de novo assembly generated the 5,805,096 bp draft genome of P. putida TJI51. Out of 6,036 protein coding genes in P. putida TJI51 sequences, 4,367 (72%) were annotated with functional specifications, while the remaining encoded hypothetical proteins. Comparative genome sequence analysis revealed that the P. putida TJI51genome contains several regions, not identified in so far sequenced P. putida genomes. Some of these regions were predicted to encode enzymes, including acetylornithine deacetylase, betaine aldehyde dehydrogenase, aldehyde dehydrogenase, benzoylformate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangifera indica; Bacterial genomics; Plant-associated bacteria; Endophyte.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300465

Association between molecular markers and behavioral phenotypes in the immatures of a butterfly
Provedor de dados: Genetics and Molecular Biology
Autores: De Nardin,Janaína; Buffon,Vanessa; Revers,Luís Fernando; de Araújo,Aldo Mellender.
Abstract Newly hatched caterpillars of the butterfly Heliconius erato phyllis routinely cannibalize eggs. In a manifestation of kin recognition they cannibalize sibling eggs less frequently than unrelated eggs. Previous work has estimated the heritability of kin recognition in H. erato phyllis to lie between 14 and 48%. It has furthermore been shown that the inheritance of kin recognition is compatible with a quantitative model with a threshold. Here we present the results of a preliminary study, in which we tested for associations between behavioral kin recognition phenotypes and AFLP and SSR markers. We implemented two experimental approaches: (1) a cannibalism test using sibling eggs only, which allowed for only two behavioral outcomes (cannibal and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kin discrimination; Caterpillar-egg cannibalism; Lepidoptera; Heliconius; AFLP.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200243

Inclusion of South American samples reveals new population structuring of the blacktip shark (Carcharhinus limbatus) in the western Atlantic
Provedor de dados: Genetics and Molecular Biology
Autores: Sodré,Davidson; Rodrigues-Filho,Luis F.S.; Souza,Rosália F.C.; Rêgo,Péricles S.; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
Carcharhinus limbatus has a cosmopolitan distribution and marked genetic structuring, mainly because of its philopatric behavior. However, analysis of this structuring has not previously included South American populations. In the present study, we analyzed a sample of adult individuals collected on the northern coast of Brazil and compared the sequences of the mitochondrial control region with those of populations already genotyped. Relatively high haplotype diversity (12 haplotypes, genetic diversity of 0.796) was observed, similar to that in other populations but with a much larger number of private alleles. In contrast to populations studied previously, which were represented by neonates, the pronounced allelic variability found in the South American...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carcharhinus limbatus; Control region; Fishery stocks; Mitochondrial DNA; Population genetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500008

Absence of the A4 peptide in the G4 glycinin subunit of soybean cultivar Enrei is caused by a point mutation in the Gy4 gene
Provedor de dados: Genetics and Molecular Biology
Autores: Yu,Kangfu; Poysa,Vaino; Haffner,Margaret; Zhang,Bailing; Woodrow,Lorna.
Functional properties of soy proteins for food are closely related to the composition of their storage protein subunits. Using base excision sequence scanning (BESS), we show that the absence of the A4 peptide in the G4 glycinin subunit of the soybean (Glycine max L.) cultivar Enrei was caused by the same point mutation in the Gy4 gene as previously reported in the soybean cultivar Raiden. Although the genetic relationship between Raiden and Enrei is not known, the same point mutation in their Gy4 genes may indicate that they probably share a related origin. The application of BESS to identify single nucleotide polymorphisms (SNPs) as co-dominant markers for marker-assisted selection (MAS) of a recessive null allele is also discussed.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Base excision sequence scanning (BESS); Marker-assisted selection (MAS); Single nucleotide polymorphisms (SNPs); Seed storage protein; Soybean.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300018

Genetic analyses of days to calving and their relationships with other traits in a Canchim cattle herd
Provedor de dados: Genetics and Molecular Biology
Autores: Mucari,Talita Buttarello; Alencar,Maurício Mello de; Barbosa,Pedro Franklin; Barbosa,Rogério Taveira.
Heritabilities and genetic correlations in a Canchim beef cattle herd were estimated using data on the following traits: male scrotal circumference at twelve months (SC12); days to first calving (DFC) for heifers; days to calving (DC) for cows; male and female weight (W) at twelve months (W12); heifer weight at the start of the first breeding season (WFBS) and at first calving (WFC); and cow weight at the start of the breeding season (WBS) and at calving (WC). Analyses of the DFC and DC traits were carried out excluding and including penalized (DFC P and DC P) non-calving females. The restricted maximum likelihood method was used, applying one-trait models for the DFC and DC analyses and two-trait models for analyses of DFC and DC combined with the other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Body weight; Days to calving; Days to first calving; Genetic correlation; Heritability; Scrotal circumference.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600007

A review of techniques for spatial modeling in geographical, conservation and landscape genetics
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz-Filho,José Alexandre Felizola; Nabout,João Carlos; Telles,Mariana Pires de Campos; Soares,Thannya Nascimento; Rangel,Thiago Fernando L.V.B.
Most evolutionary processes occur in a spatial context and several spatial analysis techniques have been employed in an exploratory context. However, the existence of autocorrelation can also perturb significance tests when data is analyzed using standard correlation and regression techniques on modeling genetic data as a function of explanatory variables. In this case, more complex models incorporating the effects of autocorrelation must be used. Here we review those models and compared their relative performances in a simple simulation, in which spatial patterns in allele frequencies were generated by a balance between random variation within populations and spatially-structured gene flow. Notwithstanding the somewhat idiosyncratic behavior of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autocorrelation; Geographical genetics; Isolation-by-distance; Landscape genetics; Spatial regression.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200001

Plant growth-promoting bacteria as inoculants in agricultural soils
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Rocheli de; Ambrosini,Adriana; Passaglia,Luciane M.P.
Abstract Plant-microbe interactions in the rhizosphere are the determinants of plant health, productivity and soil fertility. Plant growth-promoting bacteria (PGPB) are bacteria that can enhance plant growth and protect plants from disease and abiotic stresses through a wide variety of mechanisms; those that establish close associations with plants, such as the endophytes, could be more successful in plant growth promotion. Several important bacterial characteristics, such as biological nitrogen fixation, phosphate solubilization, ACC deaminase activity, and production of siderophores and phytohormones, can be assessed as plant growth promotion (PGP) traits. Bacterial inoculants can contribute to increase agronomic efficiency by reducing production costs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitrogen fixation; Siderophore production; Plant-bacteria interaction; Inoculant; Rhizosphere.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400401

Iron homeostasis related genes in rice
Provedor de dados: Genetics and Molecular Biology
Autores: Gross,Jeferson; Stein,Ricardo José; Fett-Neto,Arthur Germano; Fett,Janette Palma.
Iron is essential for plants. However, excess iron is toxic, leading to oxidative stress and decreased productivity. Therefore, plants must use finely tuned mechanisms to keep iron homeostasis in each of their organs, tissues, cells and organelles. A few of the genes involved in iron homeostasis in plants have been identified recently, and we used some of their protein sequences as queries to look for corresponding genes in the rice (Oryza sativa) genome. We have assigned possible functions to thirty-nine new rice genes. Together with four previously reported sequences, we analyzed a total of forty-three genes belonging to five known protein families: eighteen YS (Yellow Stripe), two FRO (Fe3+-chelate reductase oxidase), thirteen ZIP (Zinc regulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iron; Rice; Genome analysis; Iron transporters; Ferritin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400012

Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472
Provedor de dados: Genetics and Molecular Biology
Autores: Baraúna,Rafael A.; Santos,Agenor V.; Graças,Diego A.; Santos,Daniel M.; Ghilardi Júnior,Rubens; Pimenta,Adriano M. C.; Carepo,Marta S. P.; Schneider,Maria P.C.; Silva,Artur.
Several studies of the physiological responses of different organisms exposed to extremely low-frequency electromagnetic fields (ELF-EMF) have been described. In this work, we report the minimal effects of in situ exposure to ELF-EMF on the global protein expression of Chromobacterium violaceum using a gel-based proteomic approach. The protein expression profile was only slightly altered, with five differentially expressed proteins detected in the exposed cultures; two of these proteins (DNA-binding stress protein, Dps, and alcohol dehydrogenase) were identified by MS/MS. The enhanced expression of Dps possibly helped to prevent physical damage to DNA. Although small, the changes in protein expression observed here were probably beneficial in helping the...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: C. violaceum; Electromagnetic field; Proteomic analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200227

Estimating genomic instability mediated by Alu retroelements in breast cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Fazza,Ana Cristina; Sabino,Flavia Cal; Setta,Nathalia de; Bordin Jr.,Newton Antonio; Silva,Eloiza Helena Tajara da; Carareto,Claudia Marcia Aparecida.
Alu-PCR is a relatively simple technique that can be used to investigate genomic instability in cancer. This technique allows identification of the loss, gain or amplification of gene sequences based on the analysis of segments between two Alu elements coupled with quantitative and qualitative analyses of the profiles obtained from tumor samples, surgical margins and blood. In this work, we used Alu-PCR to identify gene alterations in ten patients with invasive ductal breast cancer. Several deletions and insertions were identified, indicating genomic instability in the tumor and adjacent normal tissue. Although not associated with specific genes, the alterations, which involved chromosomal bands 1p36.23, 1q41, 11q14.3, 13q14.2, occurred in areas of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alu-PCR; Breast cancer; Gene insertions; Gene deletions; Invasive ductal carcinoma; Recombination.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100004

Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars
Provedor de dados: Genetics and Molecular Biology
Autores: Georg-Kraemer,J.E.; Cavalli,S.S.
The amylase electrophoretic patterns of 10 Brazilian brewing-barley varieties with different malting grades and diastatic power were analyzed during the 7-day germination which occurs during the malting process. Intra and inter-variety genetic variability was observed at both the structural and regulatory level. In the first few days after germination all varieties showed a few active loci, all of them with low activity. In subsequent days, new loci became active and those already detected since early germination showed increased activity. All varieties showed a continuous increase in amylase synthesis until the 3rd and/or 4th day after germination. Some varieties maintained high amylase activity until the last day of germination, while others showed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Barley; Amylases; Isoenzymes; Structural; Regulatory genetic differences.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100012

Eucalyptus ESTs corresponding to the enzyme glutamine synthetase and the protein D1, sites of action of herbicides that cause oxidative stress
Provedor de dados: Genetics and Molecular Biology
Autores: Velini,Edivaldo Domingues; Trindade,Maria Lúcia Bueno; Alves,Elza; Catâneo,Ana Catarina; Marino,Celso Luis; Maia,Ivan de Godoy; Mori,Edson Seizo; Furtado,Edson Luiz; Guerrini,Iraê Amaral; Wilcken,Carlos Frederico.
This work was aimed at locating Eucalyptus ESTs corresponding to the GS enzyme (Glutamine Synthetase, EC = 6.3.1.2) and to the D1 protein, which are directly related to resistance to herbicides that promote oxidative stress. Glutamine Synthetase corresponds to the site of action of the herbicide glufosinate. Herbicides that belong to groups such as ureas, uracils, triazines and triazinones act on the D1-Qb complex (receptor of electrons from the Photosystem II) by inactivating it. The clusters EGEQRT3302E01.g, EGEQRT3001F12.b; EGEZLV1203B04.g; EGBGFB1211H06.g and EGEZLV1205F09.g enclosed complete sequences (with 356 amino acids) of the Glutamine Synthetase enzyme. The cluster EGEQSL1054G06.g is a consensus of four reads and enclosed a complete sequence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus; Glutamine synthetase; D1 protein; Herbicide.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400010

FISH studies in a girl with sporadic aniridia and an apparently balanced de novo t(11;13)(p13;q33) translocation detect a microdeletion involving the WAGR region
Provedor de dados: Genetics and Molecular Biology
Autores: Llerena Jr.,J.C.; Cabral de Almeida,J.C.; Bastos,E.; Crolla,J.A.
Conventional cytogenetic studies on a female infant with sporadic aniridia revealed what appeared to be a balanced de novo t(11;13) (p13;q33) translocation. Fluorescence in situ hybridization (FISH) investigations, however, detected the presence of a cryptic 11p13p14 deletion which included the WAGR region and involved approximately 7.5 Mb of DNA, including the PAX6 and WT1 genes. These results account for the patient's aniridia, and place her at high risk for developing Wilms' tumour. The absence of mental retardation in the patient suggests that the position of the distal breakpoint may also help to refine the mental retardation locus in the WAGR contiguous gene syndrome (Wilms', aniridia, genital anomalies and mental retardation).
Tipo: Info:eu-repo/semantics/report
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300006

Study of the length of the mouthparts of Africanized, Caucasian and Africanized/Caucasian honey bee crosses, and relationships between glossa size  and food gathering behavior
Provedor de dados: Genetics and Molecular Biology
Autores: Pignata,Maria Izabel Barnez; Stort,Antonio Carlos; Malaspina,Osmar.
The lengths of the mouthparts of bees, the glossa, paraglossa, stipes, galea, labial palpus, maxillary palpus, cardo, lorum, mentum and prementum, were studied in Caucasian and Africanized bees and in their F1 descendants. Only the lengths of the paraglossa, stipe, galea, mentum, prementum and maxillary palpus differed significantly between these two bee types. These six variables were studied in the F1 descendants of two types of crosses, i.e., Caucasian queens x Africanized males (cross 1) and Africanized queens x Caucasian males (cross 2). Multidimensional analyses were also performed and the generalized Mahalanobis distances (D2) between the F1 descendants and the parental lines were determined. There was an apparent dominance of Africanized bees in...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400010

Methylation of the Sox9 and Oct4 promoters and its correlation with gene expression during testicular development in the laboratory mouse
Provedor de dados: Genetics and Molecular Biology
Autores: Pamnani,Mamta; Sinha,Puja; Singh,Alka; Nara,Seema; Sachan,Manisha.
Abstract Sox9 and Oct4 are two important regulatory factors involved in mammalian development. Sox9, a member of the group E Sox transcription factor family, has a crucial role in the development of the genitourinary system, while Oct4, commonly known as octamer binding transcription factor 4, belongs to class V of the transcription family. The expression of these two proteins exhibits a dynamic pattern with regard to their expression sites and levels. The aim of this study was to investigate the role of de novo methylation in the regulation of the tissue- and site-specific expression of these proteins. The dynamics of the de novo methylation of 15 CpGs and six CpGs in Sox9 and Oct4 respectively, was studied with sodium bisulfite genomic DNA sequencing in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA methylation and development; Oct4; Promoter hypermethylation; Sox9.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300452

Genetic analysis and gene fine mapping of aroma in rice (Oryza sativa L. Cyperales, Poaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Sun,Shu Xia; Gao,Fang Yuan; Lu,Xian Jun; Wu,Xian Jun; Wang,Xu Dong; Ren,Guang Jun; Luo,Hong.
We investigated inheritance and carried out gene fine mapping of aroma in crosses between the aromatic elite hybrid rice Oryza sativa indica variety Chuanxiang-29B (Ch-29B) and the non-aromatic rice O. sativa indica variety R2 and O. sativa japonica Lemont (Le). The F1 grains and leaves were non-aromatic while the F2 non-aroma to aroma segregation pattern was 3:1. The F3 segregation ratio was consistent with the expected 1:2:1 for a single recessive aroma gene in Ch-29B. Linkage analysis between simple sequence repeat (SSR) markers and the aroma locus for the aromatic F2 plants mapped the Ch-29B aroma gene to a chromosome 8 region flanked by SSR markers RM23120 at 0.52 cM and RM3459 at 1.23 cM, a replicate F2 population confirming these results. Three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aroma; Genetic analysis; Gene fine mapping; Rice (Oryza sativa L.).
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300021

Prenatal diagnosis of fetal chromosomal abnormalities: report of an 18-year experience in a Brazilian public hospital
Provedor de dados: Genetics and Molecular Biology
Autores: Kessler,Rejane G.; Sanseverino,Maria Teresa V.; Leistner-Segal,Sandra; Magalhães,José A.A.; Giugliani,Roberto.
The study of the fetal karyotype became an important tool for the fetal diagnosis of genetic diseases in the 1970s. Although application of this test has remained very restricted in Brazil, we had 905 referrals for prenatal fetal karyotyping between 1989 and 2007. In 879 cases, a fetal karyotype was obtained. We detected 74 abnormal karyotypes (8.4%), the majority being found when the prior indication was fetal malformation. When obtaining amniotic fluid or chorionic villus samples was difficult, alternative fetal materials (urine, cystic hygroma, cystic lung, intreperitoneal and cerebrospinal fluids) were collected and we had success in obtaining karyotypes in all 13 cases. Although, the option of terminating abnormal pregnancies does not legally exist in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prenatal diagnosis; Chromosomal abnormalities; Fetal malformations.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500004

In search of essentiality: Mollicute-specific genes shared by twelve genomes
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Rangel Celso; Almeida,Darcy Fontoura de; Zaha,Arnaldo; Morais,David Anderson de Lima; Vasconcelos,Ana Tereza Ribeiro de.
Mollicutes are cell wall-less bacteria with a genome characterized by its small size. Chromosomal rearrangements help these organisms evade host immune surveillance and hence cause disease. Our goal was to determine genes shared by Mollicutes genomes using the bidirectional best hit methodology. The twelve studied Mollicutes share 210 genes, most of which (&gt; 60%) fall into the following COG categories: translation, ribosomal structure and biogenesis; DNA replication, recombination and repair; nucleotide transport and metabolism and energy production and conversion. Thirty Mollicute-specific genes were identified, 22 of them previously described as essential genes in Mycoplasma genitalium.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mollicutes; Comparative genomics; Synteny; Bidirectional best hit.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200002

Apolipoprotein E polymorphism as a predictor for cognitive decline and dementia in the Saudi general population over 65 years
Provedor de dados: Genetics and Molecular Biology
Autores: Al-Khedhairy,Abdulaziz Ali A.
Specific Apolipoprotein E (ApoE) genotypes are thought to be associated with risk for Alzheimer's disease (AD). It is essential to understand how this genetic factor affects cognitive decline and dementia in the general population. One hundred and fifty elderly persons residing at social nursing centers in different provinces of Saudi Arabia were tested for ApoE genotypes, using PCR amplification of genomic DNA followed by DNA digestion with Cfo I. All subjects were diagnosed with regard to cognitive decline and dementia. In the general Saudi population, the ApoE4 allele was found to be a weaker predictor for dementia than for AD. This may be a result of non-AD pathological processes and/or of most prevalent dementia at an age when the ApoE4 effect on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ApoE; Alzheimer's disease; Dementia; Saudi population.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300004

Chitinase activities, scab resistance, mycorrhization rates and biomass of own-rooted and grafted transgenic apple
Provedor de dados: Genetics and Molecular Biology
Autores: Schäfer,Tina; Hanke,Magda-Viola; Flachowsky,Henryk; König,Stephan; Peil,Andreas; Kaldorf,Michael; Polle,Andrea; Buscot,François.
This study investigated the impact of constitutively expressed Trichoderma atroviride genes encoding exochitinase nag70 or endochitinase ech42 in transgenic lines of the apple cultivar Pinova on the symbiosis with arbuscular mycorrhizal fungi (AMF). We compared the exo- and endochitinase activities of leaves and roots from non-transgenic Pinova and the transgenic lines T386 and T389. Local and systemic effects were examined using own-rooted trees and trees grafted onto rootstock M9. Scab susceptibility was also assessed in own-rooted and grafted trees. AMF root colonization was assessed microscopically in the roots of apple trees cultivated in pots with artificial substrate and inoculated with the AMF Glomus intraradices and Glomus mosseae. Own-rooted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple; Chitinase; Mycorrhiza; Transgenic.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300014

Genetics and human rights: Two histories: restoring genetic identity after forced disappearance and identity suppression in Argentina and after compulsory isolation for leprosy in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Penchaszadeh,Victor B.; Schuler-Faccini,Lavinia.
Over the past three decades, there has been an accelerated development of genetic technology, leading to its use in human genetic identification for many purposes. Additionally, it has been made explicit that identity is a fundamental human right. A number of historical circumstances have connected these developments. Personal identity is increasingly associated with the preservation and defense of human rights and is a tool to repair the violation of these rights, particularly the right to identity. In this article, we report the use of genetics to support the right to identity in two historical circumstances. First, we report the search, localization, DNA testing and genetic identification of 110 individuals who were appropriated as babies by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetics and human rights; Suppression of identity; Leprosy; Argentina; Brazil.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200016

CYP1A1 genotypes and haplotypes and risk of oral cancer: a case-control study in South Indians
Provedor de dados: Genetics and Molecular Biology
Autores: Balaji,Lakshmi; Singh,Krishna Balaji; Bhaskar,Lakkakula V.K.S.
The CYP1A1 gene encodes for the enzyme, aryl hydrocarbon hydroxylase, which is involved in the biotransformation of various aromatic tobacco precarcinogens. In the present study, the association between CYP1A1 gene polymorphisms (IVS1-728G &gt; A, Thr461Asn and Ile462Val), and the risk of oral cancer, was examined among 157 patients with oral cancer and 132 age-matched controls, in a south Indian population. The strength of the association between CYP1A1 variants and oral cancer was estimated by logistic regression. It was found that Thr461Asn was not polymorphic. Both IVS1-728G &gt; A and Ile462Val frequencies were consistent with Hardy-Weinberg equilibrium in the control group. There were no significant differences in genotype or haplotype frequencies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CYP1A1; Haplotypes; Allele; Oral cancer.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300004

Interethnic admixture and the evolution of Latin American populations
Provedor de dados: Genetics and Molecular Biology
Autores: Salzano,Francisco Mauro; Sans,Mónica.
A general introduction to the origins and history of Latin American populations is followed by a systematic review of the data from molecular autosomal assessments of the ethnic/continental (European, African, Amerindian) ancestries for 24 Latin American countries or territories. The data surveyed are of varying quality but provide a general picture of the present constitution of these populations. A brief discussion about the applications of these results (admixture mapping) is also provided. Latin American populations can be viewed as natural experiments for the investigation of unique anthropological and epidemiological issues.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interethnic admixture; Latin America; Population structure.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200003

Absence of TERT promoter mutations in colorectal precursor lesions and cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Cruvinel-Carloni,Adriana; Yamane,Letícia; Scapulatempo-Neto,Cristovam; Guimarães,Denise; Reis,Rui Manuel.
Abstract Hotspot mutations (c.-124bp G &gt; A and c.-146bp G &gt; A) in the promoter region of the TERT gene have been recently described in several types of solid tumors, including glioma, bladder, thyroid, liver and skin neoplasms. However, knowledge with respect to colorectal precursor lesions and cancer is scarce. In the present study we aimed to determine the frequency of hotspot TERT promoter mutations in 145 Brazilian patients, including 103 subjects with precursor lesions and 42 with colorectal carcinomas, and we associated the presence of such mutations with the patients clinical-pathological features. The mutation analysis was conclusive in 123 cases, and none of the precursor and colorectal carcinoma cases showed TERT promoter mutations. We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colorectal carcinoma; TERT promoter mutations; Precursor lesions.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100082

Effects of physical exercise on butyrylcholinesterase in obese adolescents
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Isabela M.W.; Leite,Neiva; Boberg,Dellyana; Chaves,Thais J.; Eisfeld,Gerusa M.; Eisfeld,Gisele M.; Bono,Gleyse F.; Souza,Ricardo L.R.; Furtado-Alle,Lupe.
The aim of the present study was to evaluate the effect of a 12 week program of physical exercise (PE) on butyrylcholinesterase (BChE) in obese adolescents. This study compared obese adolescents (N = 54) before and after PE, regarding the relative intensity (RI) and activity of different molecular forms (G1, G2, G4 and G1-ALB) of BChE found in plasma. Waist circumference (WC) and lipid profile were also assessed before and after PE. It was shown that before PE, mean plasma BChE activity was significantly higher in obese than in non-obese adolescents and that it was significantly reduced after PE, becoming similar to that found in non-obese adolescents. Lipid profile and WC also changed in response to PE. These results are consistent with studies that found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BChE activity; Physical exercise; Obesity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500006

Mild clinical expression of S-b thalassemia in a Brazilian patient with the b+ IVS-I-6 (T®C) mutation
Provedor de dados: Genetics and Molecular Biology
Autores: Sonati,Maria de Fátima; Kaeda,Jaspal; Kimura,Elza Miyuki; Costa,Fernando Ferreira; Luzzatto,Lucio.
We report on an eight-year-old Brazilian girl with S-b+ thalassemia. The patient had a steady 10.1 g/dl hemoglobin with 57% HbS. Direct sequence analysis of b-globin gene showed her to be heterozygous for the IVS-I-6 (T®C) mutation. This b+ thalassemia mutation, sometimes referred to as the Portuguese type, was found to be associated with the C®T polymorphism at codon 2. In combination with the bS gene, this mutation results in very mild sickle cell disease symptoms.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400002

Familial congenital cyanosis caused by Hb-M Yantai (α-76 GAC → TAC, Asp → Tyr)
Provedor de dados: Genetics and Molecular Biology
Autores: Sun,Yanbo; Wang,Pingyu; Li,Youjie; Jiao,Fei; Li,Zunling; Ma,Ying; Li,Wei; Xie,Shuyang.
Methemoglobin (Hb-M) is a rare hemoglobinopathy in China. We hereby report on a family living in Yantai, East China, with congenital cyanosis due to Hb-M mutation. The proband, a 65-year-old female, presented 63% oxygen saturation. Both Hb-M concentration and arterial oxygen saturation remained unchanged, even following intravenous treatment with methylene blue. There was also no change in blood-color (chocolate-brown) after adding 0.1% KCN. A fast-moving band (Hb-X) in hemolysates was found by cellulose acetate electrophoresis, the Hb-X/Hb-A ratio exceeding 10%. GT transition at 131nt of exon 2, although present in one of the α2-globin alleles, was not found in α1-globin alleles as a whole. This mutation leads to the aspartic acid to tyrosine substitution...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Congenital cyanosis; Hemoglobin; Methemoglobinemia; A-globin gene; China.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300009

Cross-species microsatellite amplification in South American Caimans (Caiman spp and Paleosuchus palpebrosus)
Provedor de dados: Genetics and Molecular Biology
Autores: Zucoloto,Rodrigo Barban; Villela,Priscilla Marqui Schimidt; Verdade,Luciano Martins; Coutinho,Luiz Lehmann.
Microsatellite DNA markers have been used to assess genetic diversity and to study ecological behavioral characteristics in animals. Although these markers are powerful tools, their development is labor intensive and costly. Thus, before new markers are developed it is important to prospect the use of markers from related species. In the present study we investigated the possibility of using microsatellite markers developed for Alligator mississipiensis and Caiman latirostris in South American crocodilians. Our results demonstrate the use of microsatellite markers for Paleosuchus palpebrosus, Caiman crocodilus and Caiman yacare.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; STR; Primers; Crocodilians; Alligatorinae.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100015

Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Mantovani,Monique; Abel,Luciano Douglas dos Santos; Mestriner,Carlos Alberto; Moreira-Filho,Orlando.
The composition of heterochromatin classes along the chromosomes of specimens from two populations of the fish Astyanax scabripinnis was examined using fluorescence banding with GC- and AT-DNA specific fluorochromes and fluorescence in situ hybridization (FISH) with an AT-rich satellite DNA (As51) probe. For the pericentromeric heterochromatin blocks neither GC/AT-DNA specific fluorochromes nor the FISH technique produce any response with chromosomes from either of the populations. On the other hand, the telomeric distal heterochromatin blocks of both populations fluoresced when the FISH technique was applied but showed distinct responses after GC-specific fluorochrome treatments, leading us to propose different structural arrangements of the FISH-positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Satellite DNA; Chromosome structure; Base-specific fluorochromes; Heterochromatin heterogeneity; Fluorescence in situ hybridization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400012

Implementation of a parentage control system in Portuguese beef-cattle with a panel of microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Carolino,Inês; Sousa,Conceição O.; Ferreira,Sónia; Carolino,Nuno; Silva,Fátima S.; Gama,Luís T.
A study was conducted to assess the feasibility of applying a panel of 10 microsatellite markers in parentage control of beef cattle in Portugal. In the first stage, DNA samples were collected from 475 randomly selected animals of the Charolais, Limousin and Preta breeds. Across breeds and genetic markers, means for average number of alleles, effective number of alleles, expected heterozygosity and polymorphic information content, were 8.20, 4.43, 0.733 and 0.70, respectively. Enlightenment from the various markers differed among breeds, but the set of 10 markers resulted in a combined probability above 0.9995 in the ability to exclude a random putative parent. The marker-set thus developed was later used for parentage control in a group of 140 calves from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Genetic markers; Microsatellites; Parentage control.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200017

Karyological analysis of Proechimys cuvieri and Proechimys guyannensis (Rodentia, Echimyidae) from central Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Carlos Eduardo Faresin e; Eler,Eduardo Schmidt; Silva,Maria Nazareth F. da; Feldberg,Eliana.
The aim was to characterize the karyotype of rodents of the genus Proechimys from three localities in the central Brazilian Amazon, in the search for new markers that might shed light on our understanding of the taxonomy and evolutionary history of this taxon. Two karyotypes were found, viz., 2n = 28, FN = 46 in individuals from the NRSP (Cuieiras River) and REMAN (Manaus), and 2n = 46, FN = 50 in individuals from the Balbina Hydroelectric Plant. While individuals with the karyotype with 2n = 28 chromosomes were morphologically associated with Proechimys cuvieri, their karyotype shared similarities with those of the same diploid number in two other regions. Although three karyotypes are described for Proechimys cuvieri, no geographic distribution pattern...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spiny rats; C-banding; Amazon region; Karyotypes; FISH.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100012

Smith-Lemli-Opitz syndrome: clinical and biochemical findings in Brazilian patients
Provedor de dados: Genetics and Molecular Biology
Autores: Scalco,Fernanda B.; Otto,Paulo A.; Brunetti,Iguatemy L.; Cruzes,Vania M.; Moretti-Ferreira,Danilo.
Smith-Lemli-Opitz syndrome (SLOS) or RSH syndrome comprises multiple congenital anomalies and mental retardation. The underlying defect is a deficiency in the activity of delta7-sterol reductase, which decreases cholesterol and increases 7-dehydrocholesterol (7-DHC) levels. Our aim was to identify and evaluate the frequency of SLOS manifestations in a group of Brazilian patients. Based on our own data and those reported previously, we present a simple method that allows the estimation of probabilities favoring the diagnosis of SLOS. We evaluated 30 patients clinically and determined their plasma levels of cholesterol and 7-dehydrocholesterol. In 11 patients, the diagnosis was confirmed by ultraviolet spectrophotometry (UV). Of 19 patients with normal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smith-Lemli-Opitz syndrome; Cholesterol metabolism; 7-dehydrocholesterol.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300003

Positive selection, molecular recombination structure and phylogenetic reconstruction of members of the family Tombusviridae: implication in virus taxonomy
Provedor de dados: Genetics and Molecular Biology
Autores: Boulila,Moncef.
A detailed study of putative recombination events and their evolution frequency in the whole genome of the currently known members of the family Tombusviridae, comprising 79 accessions retrieved from the international databases, was carried out by using the RECCO and RDP version 3.31β algorithms. The first program allowed the detection of potential recombination sites in seven out of eight virus genera (Aureusvirus, Avenavirus, Carmovirus, Dianthovirus, Necrovirus, Panicovirus, and Tombusvirus), the second program provided the same results except for genus Dianthovirus. On the other hand, both methods failed to detect recombination breakpoints in the genome of members of genus Machlomovirus. Furthermore, based on Fisher's Exact Test of Neutrality, positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics; Phylogeny; Recombination; Positive selection; Tombusviridae; Sequence; Taxonomy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400018

Loss of genetic variability at the transferrin locus in five hatchery stocks of tambaqui (Colossoma macropomum)
Provedor de dados: Genetics and Molecular Biology
Autores: Calcagnotto,Daniela; Toledo-Filho,Silvio de Almeida.
Knowledge and conservation of the genetic variability in stocks maintained as live gene banks have become a high priority task for Brazilian fish culture. The aim of the present survey was to assess the transferrin allelic diversity of five hatchery stocks of tambaqui (Colossoma macropomum). The tambaqui stock from Pentecoste, the oldest maintained in Brazilian hatchery stations, retained three of the six alleles detected in wild populations of tambaqui from the Amazon River. Other hatchery stocks, directly or indirectly derived from the Pentecoste stock, did not show transferrin allelic variability. Insufficient number of founders and genetic drift due to sampling errors seem to be the main causes leading to loss of genetic diversity in tambaqui hatchery...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100023

The first of a series of articles dedicated to the 60th anniversary of the Brazilian Society of Genetics (SBG)
Provedor de dados: Genetics and Molecular Biology
Autores: Salzano,Francisco Mauro; Santos,Fabrício Rodrigues dos; Hartfelder,Klaus; Menck,Carlos F.M.
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300301

Complete sequence and organization of the mitochondrial genome of Cyclemys atripons (Testudines, Geoemydidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Zhang,Li; Nie,Liu-wang; Zhang,Ying; Rui,Jin-long; Zhang,Yan-yun.
The Black Bridged Leaf Turtle, Cyclemys atripons (Testudines; Cryptodira; Geoemydidae), is a poorly known species within the genus Cyclemys. We determined the complete nucleotide sequence of the Cyclemys atripons mitochondrial genome (mtDNA) and found it to be 16,500 base pairs (bp) in length, with the genome organization, gene order and base composition being identical to that of the typical vertebrate. However, unlike for most turtle mtDNA so far reported, an extra base was not found in the NADH3 gene. The C. atripons control region of mtDNA was 981 bp long. Comparisons with three other geoemydids showed that the C. atripons control region contained a highly variable region at the 3' end composed of AT enriched tandem repeats containing a fifteen-unit...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Control region; Cyclemys atripons; Mitochondrial genome; Tandem repeats.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400027

The Brazilian Hereditary Cancer Network: historical aspects and challenges for clinical cancer genetics in the public health care system in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Ashton-Prolla,Patricia; Seuanez,Hector N.
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200163

Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome
Provedor de dados: Genetics and Molecular Biology
Autores: Tiwari,Santosh K.; Sharma,Vishwas; Sharma,Varun Kumar; Gopi,Manoj; Saikant,R; Nandan,Amrita; Bardia,Avinash; Gunisetty,Sivaram; Katikala,Prasanth; Habeeb,Md. Aejaz; Khan,Aleem A.; Habibullah,C.M.
The population of India harbors one of the world's most highly diverse gene pools, owing to the influx of successive waves of immigrants over regular periods in time. Several phylogenetic studies involving mitochondrial DNA and Y chromosomal variation have demonstrated Europeans to have been the first settlers in India. Nevertheless, certain controversy exists, due to the support given to the thesis that colonization was by the Austro-Asiatic group, prior to the Europeans. Thus, the aim was to investigate pre-historic colonization of India by anatomically modern humans, using conserved stretches of five amino acid (EPIYA) sequences in the cagA gene of Helicobacter pylori. Simultaneously, the existence of a pathogenic relationship of tyrosine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; EPIYA motifs; Tyrosine phosphorylation motifs; Haplotypes; Anatomically modern humans.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200019

Transgenic plants of Petunia hybrida harboring the CYP2E1 gene efficiently remove benzene and toluene pollutants and improve resistance to formaldehyde
Provedor de dados: Genetics and Molecular Biology
Autores: Zhang,Daoxiang; Xiang,Taihe; Peihan,Li; Bao,Lumin.
The CYP2E1 protein belongs to the P450 enzymes family and plays an important role in the metabolism of small molecular and organic pollutants. In this study we generated CYP2E1 transgenic plants of Petunia using Agrobacterium rhizogenes K599. PCR analysis confirmed that the regenerated plants contained the CYP2E1 transgene and the rolB gene of the Ri plasmid. Southern blotting revealed the presence of multiple copies of CYP2E1 in the genome of transgenic plants. Fluorescent quantitative PCR revealed exogenous CYP2E1 gene expression in CYP2E1 transgenic plants at various levels, whereas no like expression was detected in either GUS transgenic plants or wild-types. The absorption of benzene and toluene by transgenic plants was analyzed through quantitative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Petunia hybrida; CYP2E1 gene; Benzene; Toluene; Formaldehyde.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400016

The plant cell cycle: Pre-Replication complex formation and controls
Provedor de dados: Genetics and Molecular Biology
Autores: Brasil,Juliana Nogueira; Costa,Carinne N. Monteiro; Cabral,Luiz Mors; Ferreira,Paulo C. G.; Hemerly,Adriana S.
Abstract The multiplication of cells in all living organisms requires a tight regulation of DNA replication. Several mechanisms take place to ensure that the DNA is replicated faithfully and just once per cell cycle in order to originate through mitoses two new daughter cells that contain exactly the same information from the previous one. A key control mechanism that occurs before cells enter S phase is the formation of a pre-replication complex (pre-RC) that is assembled at replication origins by the sequential association of the origin recognition complex, followed by Cdt1, Cdc6 and finally MCMs, licensing DNA to start replication. The identification of pre-RC members in all animal and plant species shows that this complex is conserved in eukaryotes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pre-replication complex; A. thaliana; Cell cycle.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200276

Genotoxic action of the sesquiterpene lactone glaucolide B on mammalian cells in vitro and in vivo
Provedor de dados: Genetics and Molecular Biology
Autores: Burim,Regislaine V.; Canalle,Renata; Lopes,João L. Callegari; Takahashi,Catarina S.
Glaucolide B is a sesquiterpene lactone isolated from Vernonia eremophila Mart. (Vernonieae, Asteraceae) and has schistosomicidal, antimicrobial and analgesic activities. This study examined the cytotoxic and clastogenic activities of glaucolide B in human cultured lymphocytes and in bone marrow cells from BALB/c mice. The mitotic index (MI) and chromosomal aberrations were analyzed in both of the above systems, whereas sister chromatid exchanges (SCE) and the proliferation index (PI) were determined only in vitro. In human cultured lymphocytes, glaucolide B concentrations greater than 15 µg/ml of culture medium completely inhibited cell growth. At 4 µg/ml and 8 µg/ml of culture medium, glaucolide B significantly increased the frequency of chromosomal...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300020

Population structure, fluctuating asymmetry and genetic variability in an endemic and highly isolated Astyanax fish population (Characidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Gross,Maria Claudia; Schneider,Carlos Henrique; Matiello,Mara Cristina de Almeida; Leite,Maysa de Lima; Bertollo,Luiz Antonio Carlos; Artoni,Roberto Ferreira.
Morphological and chromosomal markers were used to infer the structure and genetic variability of a population of fish of the genus Astyanax, geographically isolated at sinkhole 2 of Vila Velha State Park, Paraná, Brazil. Two morphotypes types were observed, the standard phenotype I and phenotype II which showed an anatomical alteration probably due to an inbreeding process. Fluctuating asymmetry (FA) analysis of different characters showed low levels of morphological variation among the population from sinkhole 2 and in another population from the Tibagi river (Paraná, Brazil). The Astyanax karyotype was characterized in terms of chromosomal morphology, constitutive heterochromatin and nucleolar organizer regions. Males and females presented similar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Astyanax sp.; Fluctuating asymmetry; Karyotype structure; Triploidy; Genetic conservation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400011

In situ detection of transcripts of the myogenic factor MyoD in whole chicken embryos
Provedor de dados: Genetics and Molecular Biology
Autores: Gabriel,Jane E.; Alves,Helena J.; Alvares,Lucia E.; Schmidt,Gilberto S.; Coutinho,Luiz L.
In situ hybridization has provided insights into the molecular basis of skeletal myogenesis during embryonic development. In situ detection of different muscle-specific regulatory factors in whole embryos has been described. Spatial and temporal expression patterns of these factors differed among species. The expression pattern of MyoD in whole chicken embryos was studied via in situ hybridization using a probe obtained by the reverse transcription - polymerase chain reaction (RT-PCR) technique. In newly formed somites (embryos of stage 12), MyoD mRNA transcripts were detected along the anterior to posterior axis of somites immediately adjacent to the neural tube, whereas in mature somites (embryos of stage 24), MyoD transcripts were detected throughout...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100027

Demarcation of informative chromosomes in tropical sweet corn inbred lines using microsatellite DNA markers
Provedor de dados: Genetics and Molecular Biology
Autores: Kashiani,Pedram; Saleh,Ghizan; Panandam,Jothi Malar; Abdullah,Nur Ashikin Psyquay; Selamat,Ahmad.
A study of genetic variation among 10 pairs of chromosomes extracted from 13 tropical sweet corn inbred lines, using 99 microsatellite markers, revealed a wide range of genetic diversity. Allelic richness and the number of effective alleles per chromosome ranged from 2.78 to 4.33 and 1.96 to 3.47, respectively, with respective mean values of 3.62 and 2.73. According to the Shannon's information index (I) and Nei's gene diversity coefficient (Nei), Chromosome 10 was the most informative chromosome (I = 1.311 and Nei = 0.703), while Chromosome 2 possessed the least (I = 0.762 and Nei = 0.456). Based on linkage disequilibrium (LD) measurements for loci less than 50 cM apart on the same chromosome, all loci on Chromosomes 1, 6 and 7 were in equilibrium. Even...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite; Inbred line; Chromosome; Genetic variation; Linkage disequilibrium.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400012

Genes involved in translation of Mycoplasma hyopneumoniae and Mycoplasma synoviae
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Mônica de Oliveira; Castro,Nadya da Silva; Pereira,Maristela; Soares,Célia Maria de Almeida.
This is a report on the analysis of genes involved in translation of the complete genomes of Mycoplasma hyopneumoniae strain J and 7448 and Mycoplasma synoviae. In both genomes 31 ORFs encoding large ribosomal subunit proteins and 19 ORFs encoding small ribosomal subunit proteins were found. Ten ribosomal protein gene clusters encoding 42 ribosomal proteins were found in M. synoviae, while 8 clusters encoding 39 ribosomal proteins were found in both M. hyopneumoniae strains. The L33 gene of the M. hyopneumoniae strain 7448 presented two copies in different locations. The genes encoding initiation factors (IF-1, IF-2 and IF-3), elongation factors (EF-G, EF-Tu, EF-Ts and EF-P), and the genes encoding the ribosome recycling factor (frr) and one polypeptide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasmas; Mycoplasma synoviae; Mycoplasma hyopneumoniae; Translation; Genome.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200010

Cubic-spline interpolation to estimate effects of inbreeding on milk yield in first lactation Holstein cows
Provedor de dados: Genetics and Molecular Biology
Autores: Geha,Makram J.; Keown,Jeffrey F.; Van Vleck,L. Dale.
Milk yield records (305d, 2X, actual milk yield) of 123,639 registered first lactation Holstein cows were used to compare linear regression (y = β0 + β1X + e) ,quadratic regression, (y = β0 + β1X + β2X2 + e) cubic regression (y = β0 + β1X + β2X2 + β3X3 + e) and fixed factor models, with cubic-spline interpolation models, for estimating the effects of inbreeding on milk yield. Ten animal models, all with herd-year-season of calving as fixed effect, were compared using the Akaike corrected-Information Criterion (AICc). The cubic-spline interpolation model with seven knots had the lowest AICc, whereas for all those labeled as "traditional", AICc was higher than the best model. Results from fitting inbreeding using a cubic-spline with seven knots were compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Akaike's information criterion; Cubic-spline interpolation; Inbreeding; Milk yield.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300013

Investigation of SIRT1 gene variants in HIV-associated lipodystrophy and metabolic syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Tagliari,Carmela Farias da Silva; de Oliveira,Cáren Nunes; Vogel,Greice Meyer; da Silva,Patrícia Baptista; Linden,Rafael; Lazzaretti,Rosmeri Kuhmmer; Notti,Regina Kuhmmer; Sprinz,Eduardo; Mattevi,Vanessa Suñé.
Abstract HIV-infected individuals on chronic use of highly active antiretroviral therapy (HAART) are more likely to develop adipose tissue and metabolic disorders, such as lipodystrophy (LD) and metabolic syndrome (MetS). The development of these phenotypes is known to be multifactorial. Thus, variants in genes implicated in adipogenesis and lipid metabolism may increase susceptibility to LD and MetS. Sirtuin 1 (SIRT1) may influence the outcome of these disturbances due to its role in the regulation of transcription factors involved in energy regulation. Therefore, we genotyped four polymorphisms located in SIRT1 (rs2273773 T&gt;C, rs12413112 G&gt;A, rs7895833 A&gt;G, rs12049646 T&gt;C) in 832 HIV-infected patients receiving HAART by real-time polymerase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Lipodystrophy; Metabolic syndrome; Sirtuin 1.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100105

Christine Hackel (1955-2013)
Provedor de dados: Genetics and Molecular Biology
Autores: Steiner,Carlos Eduardo; Cavalcanti,Denise Pontes.
Tipo: Info:eu-repo/semantics/article
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100002

Genetic and ecological niche modeling of Calydorea crocoides (Iridaceae): an endemic species of Subtropical Highland Grasslands
Provedor de dados: Genetics and Molecular Biology
Autores: de Alencar,Juliana Lustosa Matos; Kaltchuk-Santos,Eliane; Fachinetto,Juliana; Tacuatiá,Luana Olinda; Forni-Martins,Eliana Regina; Stiehl-Alves,Eudes Maria; de Souza-Chies,Tatiana Teixeira.
Abstract Evolutionary analyses have been widely used for evaluation of genetic diversity of natural populations and correlate these data to the fitness of the species, especially in the case of threatened species. Calydorea crocoides occurs in a restricted area at altitudes from 800 to 1500 m in southern Brazil and is considered endangered. A study assessing genetic diversity, cytogenetic features and ecological niche was performed aiming to characterize C. crocoides by multidisciplinary approaches. Molecular data highlighted that most of the total variation (76%; p &lt; 0.001) was found within populations and the parameters of genetic diversity were high at the species level (PPB = 98.97%; I = 0.4319; h = 0.2821). Gene flow (Nm) was estimated in 0.97...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic differentiation; ISSR markers; Pollen viability; Cytogenetic analyses; Restricted distributed species.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200327

Obesity and variants of the GHRL (ghrelin) and BCHE (butyrylcholinesterase) genes
Provedor de dados: Genetics and Molecular Biology
Autores: Dantas,Vitor G.L.; Furtado-Alle,Lupe; Souza,Ricardo L.R.; Chautard-Freire-Maia,Eleidi A.
Ghrelin coded by the GHRL gene is related to weight-gain, its deactivation possibly depending on its hydrolyzation by butyrylcholinesterase (BChE) encoded by the BCHE gene, an enzyme already associated with the body mass index (BMI). The aim was to search for relationships between SNPs of the GHRL and BCHE genes with BChE activity, BMI and obesity in 144 obese and 153 nonobese Euro-Brazilian male blood donors. In the obese individuals, a significant association with higher BChE activity, in the 72LM+72MM; -116GG genotype class (GHRL and BCHE genes, respectively) was noted. No significant differences were found otherwise, through comparisons between obese and control individuals, of genotype and allele frequencies in SNPs of the GHRL gene (Arg51Gln and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BCHE gene; Body-mass index; Butyrylcholinesterase; Ghrelin; GHRL gene; Obesity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200006

Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review
Provedor de dados: Genetics and Molecular Biology
Autores: Baracat-Pereira,Maria Cristina; Barbosa,Meire de Oliveira; Magalhães Júnior,Marcos Jorge; Carrijo,Lanna Clicia; Games,Patrícia Dias; Almeida,Hebréia Oliveira; Sena Netto,José Fabiano; Pereira,Matheus Rodrigues; Barros,Everaldo Gonçalves de.
The enrichment and isolation of proteins are considered limiting steps in proteomic studies. Identification of proteins whose expression is transient, those that are of low-abundance, and of natural peptides not described in databases, is still a great challenge. Plant extracts are in general complex, and contaminants interfere with the identification of proteins involved in important physiological processes, such as plant defense against pathogens. This review discusses the challenges and strategies of separomics applied to the identification of low-abundance proteins and peptides in plants, especially in plants challenged by pathogens. Separomics is described as a group of methodological strategies for the separation of protein molecules for proteomics....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sample preparation; Complex protein extract; Subproteomes; Low-abundance proteins; Cell wall proteins.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200009

DNA lesions and repair in trypanosomatids infection
Provedor de dados: Genetics and Molecular Biology
Autores: Repolês,Bruno M.; Machado,Carlos Renato; Florentino,Pilar T.V.
Abstract Pathological processes such as bacterial, viral and parasitic infections can generate a plethora of responses such as, but not restricted to, oxidative stress that can be harmful to the host and the pathogen. This stress occurs when there is an imbalance between reactive oxygen species produced and antioxidant factors produced in response to the infection. This imbalance can lead to DNA lesions in both infected cells as well as in the pathogen. The effects of the host response on the parasite lead to several kinds of DNA damage, causing alterations in the parasite’s metabolism; the reaction and sensitivity of the parasite to these responses are related to the DNA metabolism and life cycle of each parasite. The present review will discuss the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Reactive oxygen species; DNA repair.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200311

Phage display technology: applications and innovations
Provedor de dados: Genetics and Molecular Biology
Autores: Arap,Marco Antonio.
The expression of exogenous peptides on the surface of filamentous bacteriophage was initially described by Smith in 1985. Since his first study, different molecules such as small peptides and antibodies have been displayed on coat proteins of phage, greatly expanding the applications of the technology. The past decade has seen considerable progress in the techniques and applications of phage libraries. In addition, different screening methods have allowed isolation and characterization of peptides binding to several molecules in vitro, in the context of living cells, in animals and in humans. Here we review the applications, recent innovations, and future directions of phage display technology.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phage display; Applications.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100001

In vivo chemotherapeutic insight of a novel isocoumarin (3-hexyl-5,7-dimethoxy-isochromen-1-one): Genotoxicity, cell death induction, leukometry and phagocytic evaluation
Provedor de dados: Genetics and Molecular Biology
Autores: Araújo,Flávio Henrique Souza de; Figueiredo,Débora Rojas de; Auharek,Sarah Alves; Pesarini,João Renato; Meza,Alisson; Gomes,Roberto da Silva; Monreal,Antônio Carlos Duenhas; Antoniolli-Silva,Andréia Conceição Milan Brochado; Lima,Dênis Pires de; Kassuya,Candida Aparecida Leite; Beatriz,Adilson; Oliveira,Rodrigo Juliano.
Abstract Chemotherapy is one of the major approaches for the treatment of cancer. Therefore, the development of new chemotherapy drugs is an important aspect of medicinal chemistry. Chemotherapeutic agents include isocoumarins, which are privileged structures with potential antitumoral activity. Herein, a new 3-substituted isocoumarin was synthesized from 2-iodo-3,5-dimethoxy-benzoic acid and oct-1-yne in a cross-coupling Sonogashira reaction followed by a copper iodide-catalyzed intramolecular cyclization as key step using MeOH/Et3N as the solvent system. The present study also evaluated the leukometry, phagocytic activity, genotoxic potential and cell death induction of three different doses (5 mg/kg, 10 mg/kg and 20 mg/kg) of this newly synthesized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isocoumarin synthesis; Genotoxicity; Splenic phagocytosis; Chemotherapy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400665

Green turtle (Chelonia mydas) genetic diversity at Paranaguá Estuarine Complex feeding grounds in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Jordão,Juliana Costa; Bondioli,Ana Cristina Vigliar; Guebert,Flavia Maria; Thoisy,Benoit de; Toledo,Lurdes Foresti de Almeida.
Sea turtles are marine reptiles that undertake long migrations through their life, with limited information regarding juvenile stages. Feeding grounds (FGs), where they spend most of their lives, are composed by individuals from different natal origins, known as mixed stock populations. The aim of this study was to assess genetic composition, natal origins and demographic history of juvenile green turtles (Chelonia mydas) at the Paranaguá Estuarine Complex (PEC), Brazil, considered a Natural World Heritage site. Tissue samples of stranded animals were collected (n = 60), and 700 bp mitochondrial DNA sequences were generated and compared to shorter sequences from previously published studies. Global exact tests of differentiation revealed significant...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Chelonia mydas; MtDNA; Feeding grounds; Mixed stock analysis; Connectivity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300346

Chromosomal organization and phylogenetic relationships in Hypochaeris species (Asteraceae) from Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Ruas,Claudete de Fátima; Vanzela,André L.L.; Santos,Melissa O.; Fregonezi,Jeferson N.; Ruas,Paulo Maurício; Matzenbacher,Nelson I.; Aguiar-Perecin,Margarida L.R. de.
The association of cytogenetic and molecular techniques has contributed to the analysis of chromosome organization and phylogeny in plants. The fluorochrome GC-specific CMA3, fluorescent in situ hybridization (FISH) and RAPD (Random Amplified Polymorphic DNA) markers were used to investigate chromosome structure and genetic relationships in Hypochaeris (Asteraceae). Seven species native to South America, and two species introduced from Europe (H. glabra and Hypochaeris sp) were studied. FISH with rDNA probes identified one or two loci of 18S-5.8S-25S rDNA in the South American Hypochaeris species and one locus in the European species. Only one 5S rDNA locus was seen in all species studied. Blocks of GC-rich heterochromatin (CMA-positive bands) associated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome banding; FISH; Hypochaeris; Phylogenetic relationship; RAPD.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100023

Myeloperoxidase gene-463G &gt; a polymorphism and premature coronary artery disease
Provedor de dados: Genetics and Molecular Biology
Autores: Zhong,Chen; Quanzhong,Yin; Genshan,Ma; Hua,Zhang; Ruolong,Zheng; Jiahong,Wang; Chunheng,Gao.
We investigated the association between myeloperoxidase gene -463G &gt; A polymorphism and premature coronary artery disease (CAD) in two Chinese population samples: 229 patients and 230 controls. Genotypes were determined by ligase detection reaction-polymerase chain reaction sequencing and the grouping technique. We found lower frequencies of both the A/A genotype and the A allele in patients (p &lt; 0.05). Multivariate logistic regression showed that the risk of premature CAD in subjects carrying the AA genotype was reduced by 83% in relation to individuals carrying the G/G genotype (OR = 0.172, 95% CI: 0.057-0.526, p = 0.002). Our results indicate that -463G &gt; A polymorphism of the myeloperoxidase gene is associated with premature CAD in Chinese...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Premature coronary artery disease; Myeloperoxidase; Protective genetic polymorphism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200009

Efficiency of IRAP and ITS-RFLP marker systems in accessing genetic variation of Pyrenophora graminea
Provedor de dados: Genetics and Molecular Biology
Autores: Zein,Imad; Jawhar,Mohammed; Arabi,Mohammed Imad Eddin.
The usefulness of IRAP (inter-retrotransposon amplified polymorphism) and ITS-RFLP (restriction of PCR-amplified internal transcribed spacers of the rDNA) markers in the analysis of 39 Pyrenophora graminea isolates was determined. Each marker system could discriminate between all of the isolates in detecting polymorphism, albeit with variable efficiency. IRAP and ITS-RFLP produced 85% and 77% polymorphic bands, respectively, with a corresponding mean polymorphic information content (PIC) of 0.38 and 0.36. The IRAP marker index ratio (2.41) was higher than ITS-RFLP (1.50). On one hand, the quality nature of data (QND) was higher for ITS-RFLP (0.169) than IRAP (0.093). However, correlation between both marker similarity matrices was significant (r = 0.34, p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyrenophora graminea; IRAP; Internal transcribed spacer; RDNA.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200021

Molecular characterization of glucose-6-phosphate dehydrogenase deficiency in patients from the southern Brazilian city of Porto Alegre, RS
Provedor de dados: Genetics and Molecular Biology
Autores: Castro,Simone M. de; Weber,Raquel; Matte,Úrsula; Giugliani,Roberto.
Glucose-6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49) deficiency is one of the most common human enzymopathies throughout the world. Although most affected individuals are asymptomatic, there is a risk of neonatal jaundice and acute hemolytic anemia which can be triggered by infection, some pharmaceuticals and, in older individuals, eating fava beans. We characterized the molecular basis of G6PDH deficiency in a sample of 348 adults from Porto Alegre (population about 1.5 million), the capital of the southernmost Brazilian state of Rio Grande do Sul. Genomic DNA was extracted from peripheral blood leukocytes. We studied the three G6PDH mutations that appear to be the most frequent in Southern Brazil, the G202A and A376G A minus (A-) variants and the C563T...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: G6PDH deficiency; Hemolytic anemia; Pentose pathway.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100003

Cytogenetic study of Brazilian patients with myelodysplastic syndrome (MDS)
Provedor de dados: Genetics and Molecular Biology
Autores: Borgonovo,Tamara; Ribeiro,Enilze M.S.F.; Cornélio,Déborah Afonso; Schmid-Braz,Ana Teresa; Jamur,Valderez Ravaglio; Wuicik,Lismeri; Veiga,Loraine Beatriz Acosta; Ehmke,Néria A. Maia; Pasquini,Ricardo; Cavalli,Iglenir João.
Bone marrow cytogenetic studies were performed on 93 patients with primary myelodysplastic syndrome (MDS) diagnosed at the Clinical Hospital of the Federal University of Paraná, Brazil. Chromosomal alterations were observed in 69% of the patients. Monosomy of chromosome 7, deletions of 7q, 5q, 12p and 20q, rearrangements of 11q23 and trisomies of chromosomes 8 and 21 were the most frequent abnormalities observed. Among adult patients the most frequent aberrations were rearrangements of 11q23 and 12p deletions. In the pediatric group, 5q deletions and monosomy of chromosome 7 were the most common alterations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematological disorders; Myelodysplasias; Cancer cytogenetics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500002

Evaluation of the genotoxic and antigenotoxic potential of Melissa officinalis in mice
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Natália Cassettari de; Corrêa-Angeloni,Maria Júlia Frydberg; Leffa,Daniela Dimer; Moreira,Jeverson; Nicolau,Vanessa; Amaral,Patrícia de Aguiar; Rossatto,Ângela Erna; Andrade,Vanessa Moraes de.
Melissa officinalis (L.) (Lamiaceae), a plant known as the lemon balm, is native to the east Mediterranean region and west Asia. Also found in tropical countries, such as Brazil, where it is popularly known as "erva-cidreira" or "melissa", it is widely used in aqueous- or alcoholic-extract form in the treatment of various disorders. The aim was to investigate in vivo its antigenotoxicity and antimutagenicity, as well as its genotoxic/mutagenic potential through comet and micronucleus assaying. CF-1 male mice were treated with ethanolic (Mo-EE) (250 or 500 mg/kg) or aqueous (Mo-AE) (100 mg/kg) solutions of an M. officinalis extract for 2 weeks, prior to treatment with saline or Methyl methanesulfonate (MMS) doses by intraperitoneal injection. Irrespective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melissa officinalis; Comet assay; Micronucleus test; Genotoxicity; Antigenotoxicity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200021

The sugarcane signal transduction (SUCAST) catalogue: prospecting signal transduction in sugarcane
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Glaucia Mendes; Simoes,Ana Carolina Quirino; Oliveira,Katia Cristina; Garay,Humberto Miguel; Fiorini,Leonardo Costa; Gomes,Felipe dos Santos; Nishiyama-Junior,Milton Yutaka; Silva,Aline Maria da.
EST sequencing has enabled the discovery of many new genes in a vast array of organisms, and the utility of this approach to the scientific community is greatly increased by the establishment of fully annotated databases. The present study aimed to identify sugarcane ESTs sequenced in the sugarcane expressed sequence tag (SUCEST) project (&lt;A HREF="http://sucest.lad.ic.unicamp.br/"&gt;http://sucest.lad.ic.unicamp.br&lt;/A&gt;) that corresponded to signal transduction components. We also produced a sugarcane signal transduction (SUCAST) catalogue (&lt;A HREF="http://sucest.lad.ic.unicamp.br/private/mining-reports/QG/QG-mining.htm"&gt;http://sucest.lad.ic.unicamp.br/private/mining-reports/QG/QG-mining.htm&lt;/A&gt;) that covered the main categories and...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100005

Cytogenetic divergence in two sympatric fish species of the genus Astyanax Baird and Girard, 1854 (Characiformes, Characidae) from northeastern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Medrado,Aline Souza; Ribeiro,Mavione Souza; Affonso,Paulo Roberto Antunes de Mello; Carneiro,Paulo Luíz Souza; Costa,Marco Antônio.
The fish genus Astyanax is widespread throughout the Neotropical region and is one of the most species-rich genera of the Characiformes. Cytogenetic studies of Astyanax have revealed marked intra-and interspecific diversity, with the identification of various species complexes. In this report, we describe the karyotypic structure of two sympatric species of Astyanax (Astyanax sp. and Astyanax aff. fasciatus) from the Middle Contas River basin in the northeastern Brazilian state of Bahia. Both species had 2n = 48 but differed in their karyotypic formulae. Small heterochromatic blocks and multiple nucleolar organizer regions (NORs) were identified in both species. Terminal CMA3+/DAPI-signals were observed in Astyanax sp. and A. aff. fasciatus, mostly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ag-NOR; Cytotaxonomy; Fish; Fluorochromes; South America.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500014

Biochemical and genetic analysis of butyrylcholinesterase (BChE) in a family, due to prolonged neuromuscular blockade after the use of succinylcholine
Provedor de dados: Genetics and Molecular Biology
Autores: Garcia,Daniel Fantozzi; Oliveira,Ticiano G.; Molfetta,Greice A.; Garcia,Luiz V.; Ferreira,Cristiane A.; Marques,Adriana A.; Silva Jr.,Wilson Araujo.
Butyrylcholinesterase (BChE) is a plasma enzyme that catalyzes the hydrolysis of choline esters, including the muscle-relaxant succinylcholine and mivacurium. Patients who present sustained neuromuscular blockade after using succinylcholine usually carry BChE variants with reduced enzyme activity or an acquired BChE deficiency. We report here the molecular basis of the BCHE gene underlying the slow catabolism of succinylcholine in a patient who underwent endoscopic nasal surgery. We measured the enzyme activity of BChE and extracted genomic DNA in order to study the promoter region and all exons of the BCHE gene of the patient, her parents and siblings. PCR products were sequenced and compared with reference sequences from GenBank. We detected that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hereditary hypocholinesterasemia; Butyrylcholinesterase; Succinylcholine BCHE gene; DNA polymorphism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100008

DNA methylation modulates H19 and IGF2 expression in porcine female eye
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,Dongxu; Wang,Guodong; Yang,Hao; Liu,Haibo; Li,Cuie; Li,Xiaolan; Lin,Chao; Song,Yuning; Li,Zhanjun; Liu,Dianfeng.
Abstract The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: H19/IGF2; Gene expression; DNA methylation; Parthenogenetic; Pig.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100153

Reconstruction of major maternal and paternal lineages of the Cape Muslim population
Provedor de dados: Genetics and Molecular Biology
Autores: Isaacs,Shafieka; Geduld-Ullah,Tasneem; Benjeddou,Mongi.
The earliest Cape Muslims were brought to the Cape (Cape Town -South Africa) from Africa and Asia from 1652 to 1834. They were part of an involuntary migration of slaves, political prisoners and convicts, and they contributed to the ethnic diversity of the present Cape Muslim population of South Africa. The history of the Cape Muslims has been well documented and researched however no in-depth genetic studies have been undertaken. The aim of the present study was to determine the respective African, Asian and European contributions to the mtDNA (maternal) and Y-chromosomal (paternal) gene pool of the Cape Muslim population, by analyzing DNA samples of 100 unrelated Muslim males born in the Cape Metropolitan area. A panel of six mtDNA and eight Y-chromosome...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR-RFLP; Genetic polymorphism; Mitochondrial DNA; Population genetic structure; Chromosome variations.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200005

Analysis of 31 STR loci in the genetic isolate of Carloforte (Sardinia, Italy)
Provedor de dados: Genetics and Molecular Biology
Autores: Robledo,Renato; Piras,Ignazio; Beggs,William; Calò,Carla.
The genotypes of 31 autosomal short tandem repeat loci in the population of Carloforte were analyzed, these representing a linguistic and genetic isolate located on the island of Sardinia (Italy). The markers span the entire length of chromosomes 19, 20, 21 and 22. Allele frequencies and statistical parameters were presented for all loci. Observed heterozygosity ranged from 0.279 to 0.884, and polymorphism information content from 0.552 to 0.886. All but two loci showed Hardy-Weinberg equilibrium after Bonferroni correction. The 31 short tandem repeat loci examined in the present work provide additional data on the genetic structure of the Carloforte population.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autosomal STRs; Population data; Genotyping; Carloforte; Sardinia.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300006

Comparison of radiosensitivity response to acute and chronic gamma irradiation in colored wheat
Provedor de dados: Genetics and Molecular Biology
Autores: Hong,Min Jeong; Kim,Dae Yeon; Ahn,Joon-Woo; Kang,Si-Yong; Seo,Yong Weon; Kim,Jin-Baek.
Abstract We aimed to investigate the biological responses induced by acute and chronic gamma irradiation in colored wheat seeds rich in natural antioxidants. After acute and chronic irradiation, the phenotypic effects on plant growth, germination rate, seedling height, and root length were examined, and the biochemical changes were investigated by analyzing the expression of antioxidant enzyme-related genes, antioxidant enzyme activities, and total antioxidant capacity. High dosages of chronic radiation reduced plant growth compared with the controls. Electron spin resonance measurement and 2,2-diphenyl-1-picrylhydrazyl activity analysis showed lower amount of free radicals in colored wheat seeds on chronic irradiation with low dosage of gamma rays...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute irradiation; Chronic irradiation; Wheat; Electron spin resonance; Anthocyanin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400611

Drosophila viewer: a program on the formal genetics, anatomy and developmental biology of Drosophila melanogaster for students and specialists
Provedor de dados: Genetics and Molecular Biology
Autores: Otto,P.A.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400022

Informative microsatellites for genetic population studies of black-faced lion tamarins (Leontopithecus caissara)
Provedor de dados: Genetics and Molecular Biology
Autores: Martins,Milene Moura; Galetti Junior,Pedro Manoel.
Leontopithecus caissara is a critically endangered primate species from the Brazilian Atlantic Forest. Nineteen microsatellite loci, previously developed for congeneric species, were tested with 34 L. caissara individuals from Superagüi Island. Of the 19 loci, 17 (89.4%) produced robust alleles, nine (47.4%) of these proved to be polymorphic, with a total of 23 alleles and an average of 2.56 alleles per locus. Expected and observed heterozygosity averaged 0.483 and 0.561, respectively. The exclusion power for identifying the first parent of an arbitrary offspring was 0.315 over all loci. The results thus indicate both the usefulness and limitations of these nine microsatellite loci in the genetic analysis of L. caissara, as well as their potentiality for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lion tamarins; Endangered species; Genetic diversity; New World primate; SSR transferability.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100030

Impact of NGS in the medical sciences: genetic syndromes with an increased risk of developing cancer as an example of the use of new technologies
Provedor de dados: Genetics and Molecular Biology
Autores: Lapunzina,Pablo; López,Rocío Ortiz; Rodríguez-Laguna,Lara; García-Miguel,Purificación; Martínez,Augusto Rojas; Martínez-Glez,Víctor.
The increased speed and decreasing cost of sequencing, along with an understanding of the clinical relevance of emerging information for patient management, has led to an explosion of potential applications in healthcare. Currently, SNP arrays and Next-Generation Sequencing (NGS) technologies are relatively new techniques used to scan genomes for gains and losses, losses of heterozygosity (LOH), SNPs, and indel variants as well as to perform complete sequencing of a panel of candidate genes, the entire exome (whole exome sequencing) or even the whole genome. As a result, these new high-throughput technologies have facilitated progress in the understanding and diagnosis of genetic syndromes and cancers, two disorders traditionally considered to be separate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NGS; New technologies; Developmental syndrome; Cancer predisposition.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200010

Alternatively spliced MEFV transcript lacking exon 2 and its protein isoform pyrin-2d implies an epigenetic regulation of the gene in inflammatory cell culture models
Provedor de dados: Genetics and Molecular Biology
Autores: Erdem,Gokce Celikyapi; Erdemir,Sule; Abaci,Irem; Aydin,Asli K. Kirectepe; Everest,Elif; Turanli,Eda Tahir.
Abstract The function of gene body DNA methylation in alternative splicing, and its relation to disease pathogenesis is not fully elucidated. The gene for familial Mediterranean fever (MEFV) encodes the pyrin protein and contains a 998 bp CpG island, covering the second exon, which is differentially methylated in FMF patients compared to healthy controls. Our further observation of increased exon 2-spliced MEFV transcript in leukocytes of FMF patients provoked us to test the role of exon methylation in alternative splicing using inflammatory cell culture models. First, in vitro exon methylation triggered an increased level of exon 2 exclusion using a splicing cassette in a promyelocytic leukemia cell line (HL-60). HL-60 cells subjected to methylating and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exon methylation; MEFV; Pyrin; Alternative splicing; Inflammation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400688

Modeling body size evolution in Felidae under alternative phylogenetic hypotheses
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz-Filho,José Alexandre Felizola; Nabout,João Carlos.
The use of phylogenetic comparative methods in ecological research has advanced during the last twenty years, mainly due to accurate phylogenetic reconstructions based on molecular data and computational and statistical advances. We used phylogenetic correlograms and phylogenetic eigenvector regression (PVR) to model body size evolution in 35 worldwide Felidae (Mammalia, Carnivora) species using two alternative phylogenies and published body size data. The purpose was not to contrast the phylogenetic hypotheses but to evaluate how analyses of body size evolution patterns can be affected by the phylogeny used for comparative analyses (CA). Both phylogenies produced a strong phylogenetic pattern, with closely related species having similar body sizes and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autocorrelation; Body size; Felidae; Phylogenetic eigenvector regression; Phylogenies.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100026

A new method to estimate  genetic gain in annual crops
Provedor de dados: Genetics and Molecular Biology
Autores: Breseghello,Flávio; Morais,Orlando Peixoto de; Rangel,Paulo Hideo Nakano.
The genetic gain obtained by breeding programs to improve quantitative traits may be estimated by using data from regional trials. A new statistical method for this estimate is proposed and includes four steps: a) joint analysis of regional trial data using a generalized linear model to obtain adjusted genotype means and covariance matrix of these means for the whole studied period; b) calculation of the arithmetic mean of the adjusted genotype means, exclusively for the group of genotypes evaluated each year; c) direct year comparison of the arithmetic means calculated, and d) estimation of mean genetic gain by regression. Using the generalized least squares method, a weighted estimate of mean genetic gain during the period is calculated. This method...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400024

Frequency and origin of haplotypes associated with the beta-globin gene cluster in individuals with trait and sickle cell anemia in the Atlantic and Pacific coastal regions of Colombia
Provedor de dados: Genetics and Molecular Biology
Autores: Fong,Cristian; Lizarralde-Iragorri,María Alejandra; Rojas-Gallardo,Diana; Barreto,Guillermo.
Sickle cell anemia is a genetic disease with high prevalence in people of African descent. There are five typical haplotypes associated with this disease and the haplotypes associated with the beta-globin gene cluster have been used to establish the origin of African-descendant people in America. In this work, we determined the frequency and the origin of haplotypes associated with hemoglobin S in a sample of individuals with sickle cell anemia (HbSS) and sickle cell hemoglobin trait (HbAS) in coastal regions of Colombia. Blood samples from 71 HbAS and 79 HbSS individuals were obtained. Haplotypes were determined based on the presence of variable restriction sites within the β-globin gene cluster. On the Pacific coast of Colombia the most frequent...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sickle cell anemia; HbS haplotypes; BetaS globin-gene cluster haplotypes; HbS in Colombia; Afro-Colombians.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400005

Distribution of N-acetyltransferase Type 1 (NAT1) genotypes and alleles in a Turkish population
Provedor de dados: Genetics and Molecular Biology
Autores: Arslan,Serdal; Degerli,Naci; Bardakci,Fevzi.
NAT1 is an intronless gene on chromosome 8p21.3 encoding a 290-amino-acid-long protein showing acetyltransferase activity. Some 26 alleles of NAT1 gene have been identified in human populations. In the present study we determined the distributions of NAT1 genotypes and alleles in a sample of 201 individuals from the Turkish population in Central Anatolia. The most frequent genotypes were NAT1*4/NAT1*4 (51.74%), NAT1*10/NAT1*4 (22.39%), NAT1*11/NAT1*4 (7.46), NAT1*10/NAT1*10 (3.98%). Frequencies of NAT1*3, *4 (wild-type), *10 and *11 alleles were 3.73%, 69.6%, 17.66% and 7.2%, respectively. The frequency of NAT1*11 was the highest amongst the populations studied so far, the other allele frequencies being close to those described in Caucasian populations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NAT1 gene; Genetic polymorphism; Molecular epidemiology; Turkish population.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200005

Development of microsatellite markers for the genetic analysis of Magnaporthe grisea
Provedor de dados: Genetics and Molecular Biology
Autores: Brondani,Claudio; Brondani,Rosana Pereira Vianello; Garrido,Lucas da Ressurreição; Ferreira,Márcio Elias.
An AG microsatellite-enriched genomic DNA library was constructed for Magnaporthe grisea (anamorph Pyricularia grisea), the causal agent of rice blast. Seventy-two DNA clones containing microsatellite repeats were isolated and sequenced in order to develop a series of new PCR-based molecular markers to be used in genetic studies of the fungus. Twenty-four of these clones were selected to design primer pairs for the PCR amplification of microsatellite alleles. Single spore cultures of M. grisea isolated from rice and wheat in Brazil, Colombia and China were genotyped at three microsatellite loci. Isolates from southern Brazil were predominantly monomorphic at the tested SSR loci, indicating a low level of genetic variability in these samples. However, seven...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400009

Comparative cytogenetic studies of Bufo ictericus, B. paracnemis (Amphibia, Anura) and an intermediate form in sympatry
Provedor de dados: Genetics and Molecular Biology
Autores: Azevedo,MFC; Foresti,F.; Ramos,PRR; Jim,J.
Specimens of Bufo ictericus, Bufo paracnemis and a third type, considered an intermediate subgroup between these species, were cytogenetically studied by conventional Giemsa staining, C-banding and staining of the nucleolus organizer region (NOR). The nuclear DNA content and seroproteins were also analyzed to characterize these species, and verify the possibility of hybridization between them. Karyotypes and cytogenetic markers were essentially equal on the basis of the methods used. The DNA nuclear content found was 6.25 ± 0.30 pg/DNA in Bufo ictericus; 7.57 ± 0.40 pg/DNA in Bufo paracnemis and 7.04 ± 0.29 pg/DNA in the intermediate subgroup. Eletrophoresis of total blood serum in Bufo ictericus, Bufo paracnemis and the intermediate specimens revealed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bufo; Hybridization; Cytogenetics; Nuclear DNA; Seroproteins.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300012

Mechanisms and role of microRNA deregulation in cancer onset and progression
Provedor de dados: Genetics and Molecular Biology
Autores: Palmero,Edenir Inês; Campos,Silvana Gisele P de; Campos,Marcelo; Souza,Naiara C Nogueira de; Guerreiro,Ismael Dale C.; Carvalho,Andre L.; Marques,Marcia Maria C.
MicroRNAs are key regulators of various fundamental biological processes and, although representing only a small portion of the genome, they regulate a much larger population of target genes. Mature microRNAs (miRNAs) are single-stranded RNA molecules of 20-23 nucleotide (nt) length that control gene expression in many cellular processes. These molecules typically reduce the stability of mRNAs, including those of genes that mediate processes in tumorigenesis, such as inflammation, cell cycle regulation, stress response, differentiation, apoptosis and invasion. MicroRNA targeting is mostly achieved through specific base-pairing interactions between the 5' end ('seed' region) of the miRNA and sites within coding and untranslated regions (UTRs) of mRNAs;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNA; Cancer; Genetic alteration; Epigenetic alteration; Therapeutic application.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300001

Peripheral position of CCND1 and HER-2/neu oncogenes within chromosome territories in esophageal and gastric cancers non-related to amplification and overexpression
Provedor de dados: Genetics and Molecular Biology
Autores: Bizari,Lucimari; Tajara,Eloiza Helena; Silva,Ana Elizabete.
Interphase chromosomes have been shown to occupy discrete regions of the nucleus denominated chromosome territories (CTs), their active genes being preferentially positioned on the surfaces of these CTs, where they are accessible to transcriptional machinery. By means of FISH (Fluorescence in situ Hybridization), we analyzed the CCND1 and HER-2/neu gene positions within the CTs and their relationship with gene amplification and protein over-expression in esophageal and gastric cancers. The CCND1 and HER-2/Neu genes were more often positioned at the periphery (mean frequency of 60%-83%) of the CTs in tumor tissues of the esophagus and stomach. Moreover, this positioning revealed no association with either gene amplification or the protein over-expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CCND1; HER-2/neu; Chromosome territories; Esophageal carcinoma; Gastric cancer.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200006

Comparative analysis of human and bovine protein kinases reveals unique relationship and functional diversity
Provedor de dados: Genetics and Molecular Biology
Autores: Kabir,Nuzhat N.; Kazi,Julhash U.
Reversible protein phosphorylation by protein kinases and phosphatases is a common event in various cellular processes. The eukaryotic protein kinase superfamily, which is one of the largest superfamilies of eukaryotic proteins, plays several roles in cell signaling and diseases. We identified 482 eukaryotic protein kinases and 39 atypical protein kinases in the bovine genome, by searching publicly accessible genetic-sequence databases. Bovines have 512 putative protein kinases, each orthologous to a human kinase. Whereas orthologous kinase pairs are, on an average, 90.6% identical, orthologous kinase catalytic domain pairs are, on an average, 95.9% identical at the amino acid level. This bioinformatic study of bovine protein kinases provides a suitable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EPK; Kinome; APK; Bovine.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400010

A case of prenatal diagnosis of fetal hydantoin syndrome by ultrasound
Provedor de dados: Genetics and Molecular Biology
Autores: Gollop,Thomaz Rafael; Salzo,Ivan.
Fetal hydantoin syndrome (FHS) is a set of disruptions occasionally present in fetuses exposed in utero to phenytoin or other anticonvulsants. Administration of phenytoin in early pregnancy may impair proper psychomotor performance expected for children's development. Several combined phenotypic markers delineate the syndrome, but the presence of single clinical signs is more common. There is controversy about the etiology of FHS. Associated disruptions may be related to a deficiency in a detoxifying enzyme (epoxide hydrolase), vascular problems, and/or factors not yet known. Genetic causes are believed to influence susceptibility to the drug. This text reports an unusual pattern of malformations detected in an ultrasound scan (gastroschisis, sacral...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200002

Spatial pattern and genetic diversity estimates are linked in stochastic models of population differentiation
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz-Filho,José Alexandre Felizola; Telles,Mariana Pires de Campos.
In the present study, we used both simulations and real data set analyses to show that, under stochastic processes of population differentiation, the concepts of spatial heterogeneity and spatial pattern overlap. In these processes, the proportion of variation among and within a population (measured by G ST and 1 - G ST, respectively) is correlated with the slope and intercept of a Mantel's test relating genetic and geographic distances. Beyond the conceptual interest, the inspection of the relationship between population heterogeneity and spatial pattern can be used to test departures from stochasticity in the study of population differentiation.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300007

Towards the identification of flower-specific genes in Citrus spp
Provedor de dados: Genetics and Molecular Biology
Autores: Dornelas,Marcelo Carnier; Camargo,Raquel Luciana Boscariol; Berger,Irving Joseph; Takita,Marco Aurélio.
Citrus sinensis is a perennial woody species, for which genetic approaches to the study of reproductive development are not readily amenable. Here, the usefulness of the CitEST Expressed Sequence Tag (EST) database is demonstrated as a reliable new resource for identifying novel genes exclusively related to Citrus reproductive biology. We performed the analysis of an EST dataset of the CitEST Project containing 4,330 flower-derived cDNA sequences. Relying on bioinformatics tools, sequences exclusively present in this flower-derived sequence collection were selected and used for the identification of Citrus putative flower-specific genes. Our analysis revealed several Citrus sequences showing significant similarity to conserved genes known to have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Development; Flower-specific genes; Reproduction; Transcription factors.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500005

Satellyptus: analysis and database of microsatellites from ESTs of Eucalyptus
Provedor de dados: Genetics and Molecular Biology
Autores: Ceresini,Paulo Cezar; Silva,Cristina Lacerda Soares Petrarolha; Missio,Robson Fernando; Souza,Elaine Costa; Fischer,Carlos Norberto; Guillherme,Ivan Rizzo; Gregorio,Ivo; Silva,Eloiza Helena Tajara da; Cicarelli,Regina Maria Barreto; Silva,Marco Túlio Alves da; Garcia,José Fernando; Avelar,Gustavo Arbex; Porto Neto,Laercio Ribeiro; Marçon,André Ricardo; Bacci Junior,Maurício; Marini,Danyelle Cristine.
The main goal of our research was to search for SSRs in the Eucalyptus EST FORESTs database (using a software for mining SSR-motifs). With this objective, we created a database for cataloging Eucalyptus EST-derived SSRs, and developed a bioinformatics tool, named Satellyptus, for finding and analyzing microsatellites in the Eucalyptus EST database. The search for microsatellites in the FORESTs database containing 71,115 Eucalyptus EST sequences (52.09 Mb) revealed 20,530 SSRs in 15,621 ESTs. The SSR abundance detected on the Eucalyptus ESTs database (29% or one microsatellite every four sequences) is considered very high for plants. Amongst the categories of SSR motifs, the dimeric (37%) and trimeric ones (33%) predominated. The AG/CT motif was the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics tool; Genetic markers; Gene ontology; Small sequence repeats; SSR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400014

Genetic evidence for two species of the genus Pimelodus Lacépède, 1803 (Siluriformes, Pimelodidae) in the Iguaçu River (Brazil)
Provedor de dados: Genetics and Molecular Biology
Autores: Renesto,Erasmo; Zawadzki,Cláudio Henrique; Revaldaves,Eloísa.
The existence of reproductive isolation between two morphs of catfish, endemic to the Iguaçu River (Brazil), was examined by enzyme starch gel electrophoresis. Tissues of 19 catfish (Pimelodus ortmanni) and 15 of a similar morph (Pimelodus sp.), which differs from P. ortmanni by presenting larger and more scattered dusky spots on its skin, were analyzed. A Nei's (1978) genetic identity of 0.551 was determined by the analysis of 22 enzyme loci. The loci EST*1, EST*2, GDH*1, GPI*1, GPI*2, IDH*1, MDH*1, MDH*2, and PGM*1 were fixed for different alleles in each morph, that is, no heterozygote was found for these loci. The enzymatic patterns observed for the two morphs indicate both that the taxa are reproductively isolated and that they in fact represent...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400017

Base excision repair in sugarcane
Provedor de dados: Genetics and Molecular Biology
Autores: Agnez-Lima,Lucymara F.; Medeiros,Sílvia R. Batistuzzo de; Maggi,Bruno S.; Quaresma,Giovanna A.S.
DNA damage can be induced by a large number of physical and chemical agents from the environment as well as compounds produced by cellular metabolism. This type of damage can interfere with cellular processes such as replication and transcription, resulting in cell death and/or mutations. The low frequency of mutagenesis in cells is due to the presence of enzymatic pathways which repair damaged DNA. Several DNA repair genes (mainly from bacteria, yeasts and mammals) have been cloned and their products characterized. The high conservation, especially in eukaryotes, of the majority of genes related to DNA repair argues for their importance in the maintenance of life on earth. In plants, our understanding of DNA repair pathways is still very poor, the first...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100017

Bayesian inference on field data for genetic parameters for some reproductive and related traits of Nellore cattle (Bos indicus)
Provedor de dados: Genetics and Molecular Biology
Autores: Faria,Carina Ubirajara de; Magnabosco,Cláudio de Ulhôa; Reyes,Arcadio de los; Lôbo,Raysildo Barbosa; Bezerra,Luiz Antônio Framartino; Sainz,Roberto Daniel.
We used Gibbs sampling in single and two-trait animal models to estimate genetic parameters for some reproductive and related traits of Nellore cattle (Bos indicus). Female traits were age at first calving (AFC), cumulative productivity (CP) and adult weight (AW). For males, scrotal circumferences at 365 and 450 days of age were analyzed. Gibbs sampling using the ‘Multiple Trait using Gibbs Sampling under Animal Model’ (MTGSAM) program of Van Tassel and Van Vleck was used to estimate the (co)variance components of the traits and conduct genetic analyses. Heritabilities were AFC = 0.26, AW = 0.36 and CP = 0.25 under the single-trait animal model. The mean, mode and median estimates for genetic parameters obtained from the marginal posterior distributions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bayesian inference; Genetic parameters; Heritabilities; Nellore cattle; Reproductive traits.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300008

Evidence of lack of population substructuring in the Brazilian freshwater fish Prochilodus costatus
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho-Costa,Luis F.; Hatanaka,Terumi; Galetti Jr.,Pedro M.
Curimbatá-pióa (Prochilodus costatus Valenciennes, 1850) is an endemic species from the São Francisco River Basin with migratory habits, important ecological role in the ecosystem and relevant fishing importance. The present study aimed to assess the genetic variation in P. costatus in order to investigate its population genetic structure. Genetic variation was studied at three sites downstream the Três Marias dam (Upper-middle São Francisco River, State of Minas Gerais) through six specific microsatellite loci. Fish from the three sites had quite similar genetic diversity levels and no genetic differentiation was detected, suggesting that P. costatus might represent a single reproductive unit in the studied area. Alternatively, the present study was not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prochilodontidae; Curimbatá; Microsatellites; Genetic variation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200036

Genetic variation in the Solanaceae fruit bearing species lulo and tree tomato revealed by Conserved Ortholog (COSII) markers
Provedor de dados: Genetics and Molecular Biology
Autores: Enciso-Rodríguez,Felix; Martínez,Rodrigo; Lobo,Mario; Barrero,Luz Stella.
The Lulo or naranjilla (Solanum quitoense Lam.) and the tree tomato or tamarillo (Solanum betaceum Cav. Sendt.) are both Andean tropical fruit species with high nutritional value and the potential for becoming premium products in local and export markets. Herein, we present a report on the genetic characterization of 62 accessions of lulos (n = 32) and tree tomatoes (n = 30) through the use of PCR-based markers developed from single-copy conserved orthologous genes (COSII) in other Solanaceae (Asterid) species. We successfully PCR amplified a set of these markers for lulos (34 out of 46 initially tested) and tree tomatoes (26 out of 41) for molecular studies. Six polymorphic COSII markers were found in lulo with a total of 47 alleles and five polymorphic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COSII; Genetic diversity; Lulo; Tree tomato.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200013

Molecular Cytogenetics in metaphase and interphase cells for diagnosis and prognosis in cancer and genetic research
Provedor de dados: Genetics and Molecular Biology
Autores: Mühlmann-Díaz,María.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400057

Single cell chromosome rearrangements in indivuduals with reproductive failure
Provedor de dados: Genetics and Molecular Biology
Autores: Domínguez,M.G.; Rivera,H.; Vásquez,A.I.; Ramos,A.L.
Single cell chromosome rearrangements (SCCR) are incidental findings in cell cultures. Nevertheless, some authors have implicated them in habitual abortion. Ninety individuals classified in four groups were prospectively studied: A) individuals with spontaneous abortions, malformed children or molar pregnancies (N = 36); B) azoospermic males (N = 14); C) individuals with one or more children with either de novo or inherited, constitutional, chromosome abnormalities (N = 26), and D) individuals with healthy children and no reproductive failure (control group, N = 14). Lymphocyte chromosome preparations were stained for GTG bands, and 50-100 metaphases were scored per individual. The rearrangements observed were translocations, deletions, duplications,...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100005

ITS2 variability of Biomphalaria (Mollusca, Planorbidae) species from the Paranapanema Valley (São Paulo State, Brazil): diversity patterns, population structure, and phylogenetic relationships
Provedor de dados: Genetics and Molecular Biology
Autores: Tuan,Roseli; Santos,Priscila dos.
The ribosomal DNA internal transcribed spacer 2 (ITS2) has been shown to be a useful genetic marker for species identification and phylogenetic reconstruction in the genus of freshwater snails Biomphalaria (Preston 1910). Additionally, ITS2 studies in Biomphalaria have uncovered significant intra-specific genetic variability suggesting the presence of cryptic species complexes. We obtained ITS2 sequences for the Biomphalaria species B. glabrata, B. tenagophila, B. occidentalis and B. peregrina from the Paranapanema Valley (São Paulo State, Brazil) and compared them with a comprehensive set of published Biomphalaria ITS2 sequences using Bayesian inference of phylogeny. Analysis of the resulting trees showed that the newly obtained B. glabrata sequences did...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bayesian inference; Biomphalaria; DNA sequences; Internal transcribed spacer 2 (ITS2); Ribosomal DNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100024

Valongo, genetic studies on an isolated Afro-Brazilian community
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Ilíada Rainha de; Culpi,Lodércio.
A southern Brazilian isolated community of predominantly sub-Saharan African origin, with a total population of 74 individuals and high degree of inbreeding (F = 0.081) was studied. The small sizes of the breeding (35) and effective (21) populations, as well as the very small effective migration rate (4%), suggest a high probability for the occurrence of genetic drift. A sample was typed for fourteen blood genetic systems and most of these systems seem to reveal the founder effect. This evolutionary factor was probably responsible for the absence of some polymorphic alleles frequent in African populations, i.e.: ABO*B, RHD-RHCE*DCe, GPA-GPB*NS (MNSs*NS), GPA-GPB*NS U (MNSs*NSU), HBB*S, HP*2M and ESD*2. The most unusual allele frequency was that for BCHE*A,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Isolated community; Polymorphism; Random genetic drift; Blood systems; Admixture.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300012

[RETRACTED ARTICLE]: Expression of serum microRNA-378 and its clinical significance in renal cell carcinoma
Provedor de dados: Genetics and Molecular Biology
Autores: Shi,Lixin; Zhang,Lei; Wang,Chunyang; Sun,Shengkun; Cao,Xiyuan; Zhang,Xu.
Abstract Studies have demonstrated that miRNA-378 is expressed in various malignant tumors. In the present study, we aimed to explore the expression of serum miRNA-378 and its clinical significance in renal cell carcinoma (RCC) patients. A total of 75 RCC patients, 63 renal cysts (RC) patients and 75 healthy controls were selected. The miRNA-378 level in RCC and RC groups was significantly higher than in healthy control group, with RCC group having the highest level. The miRNA-378 levels were significantly decreased within the same group after surgery. When compared with healthy controls, RC group had higher levels but not significantly (p &gt; 0.05) while levels in RCC group were significantly higher (p &lt; 0.05). miRNA-378 expression was correlated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal cell carcinoma; MicroRNA 378; Diagnosis; Patients; AUC.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300525

Karyotype characterization of Trigona fulviventris Guérin, 1835 (Hymenoptera, Meliponini) by C banding and fluorochrome staining: report of a new chromosome number in the genus
Provedor de dados: Genetics and Molecular Biology
Autores: Domingues,Alayne Magalhães Trindade; Waldschmidt,Ana Maria; Andrade,Sintia Emmanuelle; Andrade-Souza,Vanderly; Alves,Rogério Marco de Oliveira; Silva Junior,Juvenal Cordeiro da; Costa,Marco Antônio.
Although many species of the genus Trigona have been taxonomically described, cytogenetic studies of these species are still rare. The aim of the present study was to obtain cytogenetic data by conventional staining, C banding and fluorochrome staining for the karyotype characterization of the species Trigona fulviventris. Cytogenetic analysis revealed that this species possesses a diploid chromosome number of 2n = 32, different from most other species of this genus studied so far. This variation was probably due to the centric fusion in a higher numbered ancestral karyotype, this fusion producing the large metacentric chromosome pair and the lower chromosome number observed in Trigona fulviventris. Heterochromatin was detected in the pericentromeric...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytogenetics; Heterochromatin; Evolution; Stingless bees.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300009

Image analysis of DNA fragmentation and loss in V79 cells under apoptosis
Provedor de dados: Genetics and Molecular Biology
Autores: Maria,Silvya Stuchi; Vidal,Benedicto de Campos; Mello,Maria Luiza S.
Nuclear image analysis of Feulgen-stained V79 fibroblasts after three days in culture was used to discriminate apoptotic cells and cells suspected to be undergoing apoptosis from control cells based on parameters such as the Feulgen-DNA content, degree of chromatin condensation and nuclear areas, in association with visual morphology. The fibroblasts were initially plated at a density of 10(5) cells/ml and incubated under optimal culture conditions without subculturing. Following confluency, the cells underwent contact inhibition apoptosis. Image analysis revealed three nuclear phenotypes which were defined in terms of their morphological characteristics and levels of chromatin condensation. A decrease in the amount of Feulgen-DNA was detected in apoptotic...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100020

A walk on the wild side: Oryza species as source for rice abiotic stress tolerance
Provedor de dados: Genetics and Molecular Biology
Autores: Menguer,Paloma Koprovski; Sperotto,Raul Antonio; Ricachenevsky,Felipe Klein.
Abstract Oryza sativa, the common cultivated rice, is one of the most important crops for human consumption, but production is increasingly threatened by abiotic stresses. Although many efforts have resulted in breeding rice cultivars that are relatively tolerant to their local environments, climate changes and population increase are expected to soon call for new, fast generation of stress tolerant rice germplasm, and current within-species rice diversity might not be enough to overcome such needs. The Oryza genus contains other 23 wild species, with only Oryza glaberrima being also domesticated. Rice domestication was performed with a narrow genetic diversity, and the other Oryza species are a virtually untapped genetic resource for rice stress tolerance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza; Rice; Wild species; Abiotic stress; Domestication.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200238

Identification of selection signatures in livestock species
Provedor de dados: Genetics and Molecular Biology
Autores: Gouveia,João José de Simoni; Silva,Marcos Vinicius Gualberto Barbosa da; Paiva,Samuel Rezende; Oliveira,Sônia Maria Pinheiro de.
The identification of regions that have undergone selection is one of the principal goals of theoretical and applied evolutionary genetics. Such studies can also provide information about the evolutionary processes involved in shaping genomes, as well as physical and functional information about genes/genomic regions. Domestication followed by breed formation and selection schemes has allowed the formation of very diverse livestock breeds adapted to a wide variety of environments and with special characteristics. The advances in genomics in the last five years have enabled the development of several methods to detect selection signatures and have resulted in the publication of a considerable number of studies involving livestock species. The aims of this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artificial selection; Domestic animals; Selective sweep.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300004

Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae
Provedor de dados: Genetics and Molecular Biology
Autores: Valadares-Inglis,Maria Cléria; Peberdy,John F.
Pulsed-field gel electrophoresis (PFGE) was used to separate chromosome-sized DNA molecules of four strains of Metarhizium anisopliae from Brazil. Metarhizium anisopliae isolates from Japan have been reported as possessing seven chromosomes. Variation was observed among the Brazilian strains and the chromosomal DNA was resolved into eight bands for strain CG46. Densitometric analysis of PFGE gels suggested that the other three Brazilian strains also possess eight chromosomes, with two chromosomes migrating as doublets under the electrophoretic conditions used. The genome size was estimated as varying between 23.39 to 31.88 Mb, not including possible doublet chromosomes.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100003

Relationship between leaf stages and epistasis for resistance to Stagonospora nodorum in durum wheat
Provedor de dados: Genetics and Molecular Biology
Autores: Bnejdi,Fethi; Saadoun,Mourad; Naouari,Mouna; El Gazzah,Mohamed.
Ten varieties and eight generations (2F1, 2F2, 2B1 and 2B2) of durum wheat derived from two crosses were evaluated for resistance to natural infection by Stagonospora nodorum blotch (SNB) at the 2-3 and 6-7 leaf stages at two sites over two years. There were significant differences in the incidence of SNB between leaf stages in most of the wheat varieties, with resistance being most evident at the 6-7 leaf stage. Separate analyses of the mean values for each generation showed that the genetic mechanism of defense against the pathogen depended upon the leaf stage. At the 2-3 leaf stage, only additive and dominance effects were implicated in the control of SNB for the two crosses at the two sites and for the two replications. For the 6-7 leaf stage,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adaptability; Inheritance; Leaf stage; Stagonospora nodorum blotch.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300010

Mitochondrial DNA phylogeography of Brazilian populations of Drosophila buzzatii
Provedor de dados: Genetics and Molecular Biology
Autores: Brito,Reinaldo A. de; Manfrin,Maura H.; Sene,Fabio M.
The Argentinean Chaco region has been considered the center of origin of Drosophila buzzatii in South America because it contains most of the chromosomal polymorphism detected in natural populations. Two hypotheses have been put forward to explain the distribution of D. buzzatii in Brazil, one proposing that it has only recently passively colonized Brazil via human dispersal and the other suggesting that D. buzzatii has actively migrated to Brazil some time ago. Data from chromosomal inversions support recent colonization, whereas data from allozymes and mtDNA variation indicate that D. buzzatii has been in Brazil longer, favoring an active dispersal hypothesis. In our present work we analyzed data on 56 South American flies, mostly from Brazil, sequenced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila buzzatii; Population structure; Mitochondrial DNA; Phylogeography; Brazil; Climatic cycles.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200009

Identification and characterization of polymorphisms at the HSA alpha1-acid glycoprotein (ORM*) gene locus in Caucasians
Provedor de dados: Genetics and Molecular Biology
Autores: Owczarek,Catherine M.; Owczarek,Aleksander L.; Board,Philip G.
Human alpha1-acid glycoprotein (AGP) or orosomucoid (ORM) is a major acute phase protein that is thought to play a crucial role in maintaining homeostasis. Human AGP is the product of a cluster of at least two adjacent genes located on HSA chromosome 9. Using a range of restriction endonucleases we have investigated DNA variation at the locus encoding the AGP genes in a panel of healthy Caucasians. Polymorphisms were identified using BamHI, EcoRI, BglII, PvuII, HindIII, TaqI and MspI. Non-random associations were found between the BamHI, EcoRI, BglII RFLPs. The RFLPs detected with PvuII, TaqI and MspI were all located in exon 6 of both AGP genes. The duplication of an AGP gene was observed in 11% of the indiviuals studied and was in linkage disequilibrium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human alpha1-acid glycoprotein; RFLP; Linkage disequilibrium.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100004

Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cells
Provedor de dados: Genetics and Molecular Biology
Autores: Drets,Máximo E.; Mendizábal,Marcos.
Microphotometrical scanning and computer graphic image analysis were carried out to detect the distribution of chromatin densities in subtelomeric segments of T-banded endoreduplicated chromosomes of Chinese hamster ovary (CHO) cells. Chromatin density patterns detected with this method were similar to those previously found in CHO and normal human chromosomes. The highest chromatin densities were considered as marker segments which led to the detection of reciprocal changes of position in endoreduplicated chromosomes during cell spreading on the slide. The problem of the subtelomeric T-banding density patterns found in the endoreduplicated chromosomes and their relation to the structure and molecular composition of this region is briefly discussed.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200008

Comparative proteomic analysis of indica and japonica rice varieties
Provedor de dados: Genetics and Molecular Biology
Autores: Yang,Yanhua; Zhu,Keming; Xia,Hengchuan; Chen,Liang; Chen,Keping.
Indica and japonica are two main subspecies of Asian cultivated rice (Oryza sativa L.) that differ clearly in morphological and agronomic traits, in physiological and biochemical characteristics and in their genomic structure. However, the proteins and genes responsible for these differences remain poorly characterized. In this study, proteomic tools, including two-dimensional electrophoresis and mass spectrometry, were used to globally identify proteins that differed between two sequenced rice varieties (93-11 and Nipponbare). In all, 47 proteins that differed significantly between 93-11 and Nipponbare were identified using mass spectrometry and database searches. Interestingly, seven proteins were expressed only in Nipponbare and one protein was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indica and japonica rice; Molecular marker; Proteomics; Quantitative real-time PCR; Unique proteins.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500008

Elimination of micronuclei from microspores in a Brazilian oat (Avena sativa L.) variety
Provedor de dados: Genetics and Molecular Biology
Autores: Baptista-Giacomelli,Flávia Roseli; Pagliarini,Maria Suely; Almeida,Juliano Luiz de.
Chromosome elimination in interspecific hybrids is a powerful tool in breeding programs. Of 12 Brazilian oat varieties recommended for cultivation in southern Brazil, one variety, UFRGS 15, presented a new type of chromosome elimination never described in any other species. Chromosomes that produced micronuclei during meiosis were eliminated from microspores as microcytes. The micronucleus reached the microspore wall and formed a kind of bud, separated from the microspore. The eliminated microcytes gave origin to small and sterile pollen grains. The possible applications of this finding for oat breeding are discussed.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300029

Expression of sugarcane genes induced by inoculation with Gluconacetobacter diazotrophicus and Herbaspirillum rubrisubalbicans
Provedor de dados: Genetics and Molecular Biology
Autores: Nogueira,Eduardo de Matos; Vinagre,Fabiano; Masuda,Hana Paula; Vargas,Claudia; Pádua,Vânia Lúcia Muniz de; Silva,Felipe Rodrigues da; Santos,Renato V. dos; Baldani,José Ivo; Ferreira,Paulo Cavalcanti Gomes; Hemerly,Adriana Silva.
Several Brazilian sugarcane varieties have the ability to grow with little addition of inorganic nitrogen fertilizers, showing high contributions of Biological Nitrogen Fixation (BNF). A particular type of nitrogen-fixing association has been described in this crop, where endophytic diazotrophs such as Gluconacetobacter diazotrophicus and Herbaspirillum spp. colonize plant tissues without causing disease symptoms. In order to gain insight into the role played by the sugarcane in the interaction between this plant and endophytic diazotrophs, we investigated gene expression profiles of sugarcane plants colonized by G. diazotrophicus and H. rubrisubalbicans by searching the sugarcane expressed sequence tag SUCEST Database (&lt;A...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100027

Intra- and inter-individual chromosome variation in Hoplerythrinus unitaeniatus (Pisces, Erythrinidae): a population from the Brazilian São Francisco river basin
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz,Débora; Bertollo,Luiz A.C.
Chromosomal studies were carried out on a population of the fish Hoplerythrinus unitaeniatus (jeju) from Prata river in the São Francisco river basin, Minas Gerais, Brazil using conventional Giemsa staining, C-banding, silver nitrate nucleolar organizer region (Ag-NOR) staining and fluorescent in situ hybridization (FISH) with 18S and 5S rDNA probes. We found a high degree of inter- and intra-individual variability with the identification of 2n = 50, 2n = 51 and 2n = 52 karyotypes at nearly the same frequency within the population. Intra-individual variation in chromosomal morphology and, consequently, karyotype formulae was also observed, chiefly in the specimens with 2n = 50 and 2n = 52 chromosomes. Ag-NORs and 18S rDNA sites also showed numerical and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotypes; C-banding; Nucleolar organizing regions; Intra- and inter-individual polymorphism.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300009

Clinical research challenges in rare genetic diseases in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Giugliani,Luciana; Vanzella,Claudia; Zambrano,Marina Bauer; Donis,Karina Carvalho; Wallau,Thaís Klassmann Wendland; Costa,Fernando Machado da; Giugliani,Roberto.
Abstract Rare diseases are defined as conditions with a prevalence of no more than 6.5 per 10,000 people. Although each rare disease individually affects a small number of people, collectively, the 6,000 to 8,000 rare conditions (80% of them with genetic cause) affect around 8% of the world’s population. Research about the natural history and underlying pathophysiological mechanisms of rare diseases, as well as clinical trials with new drugs, are important and necessary to develop new strategies for the treatment of these conditions. This report describes the experience of a clinical research group working with rare diseases in a reference center for lysosomal diseases in Brazil (Medical Genetics Service, Hospital de Clínicas de Porto Alegre). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clinical research; Clinical investigation; Rare diseases; Lysosomal storage diseases; Enzyme replacement therapy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200305

Lack of mutations in the PVRL3 gene in North American caucasians with non-syndromic cleft lip/palate
Provedor de dados: Genetics and Molecular Biology
Autores: Sözen,Mehmet A.; Hecht,Jacqueline T.; Spritz,Richard A.
Cleft lip with or without cleft palate (CLP) is one of the most common birth defects. In about 70% of cases, CLP occurs as an isolated anomaly, denoted non-syndromic CLP (nsCLP). Genetic linkage and association studies have implicated many loci in susceptibility to nsCLP, including some members of the nectin gene family. We performed mutation screening of the PVRL3 gene that encodes nectin-3 in 73 unrelated Caucasian nsCLP patients and 105 unrelated controls from North America. We detected no sequence variants in the PVRL3 gene in either the nsCLP patients or the controls. These data suggest that PVRL3 is not an important susceptibility gene for nsCLP in the North American Caucasian population.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orofacial clefts; Nectins; PVRL3; Mutation analysis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400008

Molecular cloning and expression profiling of a chalcone synthase gene from hairy root cultures of Scutellaria viscidula Bunge
Provedor de dados: Genetics and Molecular Biology
Autores: Lei,Wei; Tang,Shao-Hu; Luo,Ke-Ming; Sun,Min.
A cDNA encoding chalcone synthase (CHS), the key enzyme in flavonoid biosynthesis, was isolated from hairy root cultures of Scutellaria viscidula Bunge by rapid amplification of cDNA ends (RACE). The full-length cDNA of S. viscidula CHS, designated as Svchs (GenBank accession no. EU386767), was 1649 bp with a 1170 bp open reading frame (ORF) that corresponded to a deduced protein of 390 amino acid residues, a calculated molecular mass of 42.56 kDa and a theoretical isoelectric point (pI) of 5.79. Multiple sequence alignments showed that SvCHS shared high homology with CHS from other plants. Functional analysis in silico indicated that SvCHS was a hydrophilic protein most likely associated with intermediate metabolism. The active sites of the malonyl-CoA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chalcone synthase gene; Methyl jasmonate; Molecular cloning; Scutellaria viscidula Bunge.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200015

Polymorphism of alpha-1-antitrypsin in hematological malignancies
Provedor de dados: Genetics and Molecular Biology
Autores: Topic,Aleksandra; Juranic,Zorica; Jelic,Svetislav; Magazinovic,Ivana Golubicic.
Alpha-1-antitrypsin (AAT) or serine protease inhibitor A1 (SERPINA1) is an important serine protease inhibitor in humans. The main physiological role of AAT is to inhibit neutrophil elastase (NE) released from triggered neutrophils, with an additional lesser role in the defense against damage inflicted by other serine proteases, such as cathepsin G and proteinase 3. Although there is a reported association between AAT polymorphism and different types of cancer, this association with hematological malignancies (HM) is, as yet, unknown. We identified AAT phenotypes by isoelectric focusing (in the pH 4.2-4.9 range) in 151 serum samples from patients with HM (Hodgkins lymphomas, non-Hodgkins lymphomas and malignant monoclonal gammopathies). Healthy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-1-antitrypsin; Polymorphism; Lymphomas.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400008

Significant genetic differentiation among populations of Anomalocardia brasiliana (Gmelin, 1791): a bivalve with planktonic larval dispersion
Provedor de dados: Genetics and Molecular Biology
Autores: Arruda,Cinthya Cristina Bulhões; Beasley,Colin Robert; Vallinoto,Marcelo; Marques-Silva,Nelane do Socorro; Tagliaro,Claudia Helena.
Four Brazilian populations of Anomalocardia brasiliana were tested for mutual genetic homogeneity, using data from 123 sequences of the mtDNA cytochrome oxidase c subunit I gene. A total of 36 haplotypes were identified, those shared being H3 (Canela Island, Prainha and Acupe) and both H5 and H9 (Prainha and Acupe). Haplotype diversity values were high, except for the Camurupim population, whereas nucleotide values were low in all the populations, except for that of Acupe. Only the Prainha population showed a deviation from neutrality and the SSD test did not reject the demographic expansion hypothesis. Fst values showed that the Prainha and Acupe populations represent a single stock, whereas in both the Canela Island and Camurupim stocks, population...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; COI; Population; Anomalocardia brasiliana; Conservation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200033

Site heteroplasmy in the mitochondrial cytochrome b gene of the sterlet sturgeon Acipenser ruthenus
Provedor de dados: Genetics and Molecular Biology
Autores: Dudu,Andreea; Georgescu,Sergiu Emil; Berrebi,Patrick; Costache,Marieta.
Sturgeons are fish species with a complex biology. They are also characterized by complex aspects including polyploidization and easiness of hybridization. As with most of the Ponto-Caspian sturgeons, the populations of Acipenser ruthenus from the Danube have declined drastically during the last decades. This is the first report on mitochondrial point heteroplasmy in the cytochrome b gene of this species. The 1141 bp sequence of the cytb gene in wild sterlet sturgeon individuals from the Lower Danube was determined, and site heteroplasmy evidenced in three of the 30 specimens collected. Two nucleotide sequences were identified in these heteroplasmic individuals. The majority of the heteroplasmic sites are synonymous and do not modify the sequence of amino...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sterlet sturgeon; MtDNA; Site heteroplasmy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500024

Use of the heteroduplex mobility assay and cell sorting to select genome sequences of the CCR5 gene in HEK 293T cells edited by transcription activator-like effector nucleases
Provedor de dados: Genetics and Molecular Biology
Autores: Nerys-Junior,Arildo; Costa,Lendel C.; Braga-Dias,Luciene P.; Oliveira,Márcia; Rossi,Átila D.; Cunha,Rodrigo Delvecchio da; Gonçalves,Gabriel S.; Tanuri,Amilcar.
Engineered nucleases such as zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN) are one of the most promising tools for modifying genomes. These site-specific enzymes cause double- strand breaks that allow gene disruption or gene insertion, thereby facilitating genetic manipulation. The major problem associated with this approach is the labor-intensive procedures required to screen and confirm the cellular modification by nucleases. In this work, we produced a TALEN that targets the human CCR5 gene and developed a heteroduplex mobility assay for HEK 293T cells to select positive colonies for sequencing. This approach provides a useful tool for the quick detection and easy assessment of nuclease activity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CCR5; Genome editing; Gene knockout; TALEN; HMA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100018

Mutagenic activity of airborne particulate matter from the urban area of Porto Alegre, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Vargas,Vera Maria Ferrão; Horn,Rubem Cesar; Guidobono,Régis Rolim; Mittelstaedt,Ana Beatriz; Azevedo,Irascema Girardi de.
The mutagenic activity of airborne particulate matter collected from three different sites within the urban area of Porto Alegre, Brazil, was investigated using a Salmonella/microsome assay. Samples were extracted by sonication, sequentially, with cyclohexane (CX), and dichloromethane (DCM), for a rough fractionation by polarity. The different fractions were tested for mutagenicity using Salmonella typhimurium strains TA98, with and without metabolic activation (S9 mix fraction), and TA98NR and TA98/1,8-DNP6, without metabolic activation. Mutagenic response was observed for frameshift strain TA98 in assays with and without metabolization for two sites (sites 2 and 3), which had considerable risk of environmental contamination by nonpolar (CX) and/or...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200013

Cockayne syndrome: report of a Brazilian family with confirmation of impaired RNA synthesis after UV-irradiation
Provedor de dados: Genetics and Molecular Biology
Autores: Karam,Simone M.; Costa,Jaderson C.; Jardim,Laura; Pires,Ricardo F.; Lehmann,Alan R.; Giugliani,Roberto.
Cockayne syndrome (CS) is an autosomal recessive disorder characterized by dwarfism, growth deficiency, neurological deterioration, skin photosensitivity and a characteristic progressive facial appearance. In the present study we report the first Brazilian CS family in which diagnosis was confirmed by the demonstration of decreased RNA synthesis in cultured fibroblasts exposed to UV-C radiation. Despite the progressive course of the disease and the unavailability of an effective treatment, diagnosis may be very important for the benefits to be gained by the afflicted family from genetic counseling and/or prenatal diagnosis.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200005

Analysis of NOR distribution in cultivated and naturalized stocks of rainbow trout (Oncorhynchus mykiss)
Provedor de dados: Genetics and Molecular Biology
Autores: Porto-Foresti,Fábio; Oliveira,Claudio; Tabata,Yara A.; Rigolino,Marcos G.; Foresti,Fausto.
In salmonid species, nucleolar organizing regions (NORs) have frequently been found on a single chromosome pair. However, some other, smaller yet active NORs have also been described. In the present study, NORs were found to be located in different positions on the chromosomes of 12 rainbow trout samples (11 cultivated and one naturalized stocks). Three phenotypes were found: 1) NORs located on two submetacentric chromosomes, in a subterminal position on the short arm (AA); 2) NORs located on two submetacentric chromosomes, in a subterminal position on the long arm (BB); and 3) NORs located on two submetacentric chromosomes, one in a subterminal position on the short arm, and the other in a subterminal position on the long arm (AB). Phenotype AA was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NOR polymorphism; Fish cytogenetics; Rainbow trout.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400009

TSC1 and TSC2 gene mutations and their implications for treatment in Tuberous Sclerosis Complex: a review
Provedor de dados: Genetics and Molecular Biology
Autores: Rosset,Clévia; Netto,Cristina Brinckmann Oliveira; Ashton-Prolla,Patricia.
Abstract Tuberous sclerosis complex is an autosomal dominant disorder characterized by skin manifestations and formation of multiple tumors in different organs, mainly in the central nervous system. Tuberous sclerosis is caused by the mutation of one of two tumor suppressor genes, TSC1 or TSC2. Currently, the development of novel techniques and great advances in high-throughput genetic analysis made mutation screening of the TSC1 and TSC2 genes more widely available. Extensive studies of the TSC1 and TSC2 genes in patients with TSC worldwide have revealed a wide spectrum of mutations. Consequently, the discovery of the underlying genetic defects in TSC has furthered our understanding of this complex genetic disorder, and genotype-phenotype correlations are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberous sclerosis complex; TSC mutations; Genotype-phenotype correlations; TSC1; TSC2.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100069

Galactinol synthase transcriptional profile in two genotypes of Coffea canephora with contrasting tolerance to drought
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Tiago Benedito Dos; Lima,Rogério Barbosa de; Nagashima,Getúlio Takashi; Petkowicz,Carmen Lucia de Oliveira; Carpentieri-Pípolo,Valéria; Pereira,Luiz Filipe Protasio; Domingues,Douglas Silva; Vieira,Luiz Gonzaga Esteves.
Increased synthesis of galactinol and raffinose family oligosaccharides (RFOs) has been reported in vegetative tissues in response to a range of abiotic stresses. In this work, we evaluated the transcriptional profile of a Coffea canephora galactinol synthase gene (CcGolS1) in two clones that differed in tolerance to water deficit in order to assess the contribution of this gene to drought tolerance. The expression of CcGolS1 in leaves was differentially regulated by water deficit, depending on the intensity of stress and the genotype. In clone 109A (drought-susceptible), the abundance of CcGolS1 transcripts decreased upon exposure to drought, reaching minimum values during recovery from severe water deficit and stress. In contrast, CcGolS1 gene expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Drought stress; Raffinose family oligosaccharides (RFOs).
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200182

Genetic association of single nucleotide polymorphisms in dystrobrevin binding protein 1 gene with schizophrenia in a Malaysian population
Provedor de dados: Genetics and Molecular Biology
Autores: Tan,Grace Kang Ning; Tee,Shiau Foon; Tang,Pek Yee.
Dystrobrevin binding protein 1 (DTNBP1) gene is pivotal in regulating the glutamatergic system. Genetic variants of the DTNBP1 affect cognition and thus may be particularly relevant to schizophrenia. We therefore evaluated the association of six single nucleotide polymorphisms (SNPs) with schizophrenia in a Malaysian population (171 cases; 171 controls). Associations between these six SNPs and schizophrenia were tested in two stages. Association signals with p &lt; 0.05 and minor allele frequency &gt; 0.05 in stage 1 were followed by genotyping the SNPs in a replication phase (stage 2). Genotyping was performed with sequenced specific primer (PCR-SSP) and restriction fragment length polymorphism (PCR-RFLP). In our sample, we found significant associations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Case-control study; DTNBP1; Schizophrenia; Single nucleotide polymorphism.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200138

Comparison of multiple genotyping methods for the identification of the cancer predisposing founder mutation p.R337H in TP53
Provedor de dados: Genetics and Molecular Biology
Autores: Fitarelli-Kiehl,Mariana; Macedo,Gabriel S.; Schlatter,Rosane Paixão; Koehler-Santos,Patricia; Matte,Ursula da Silveira; Ashton-Prolla,Patricia; Giacomazzi,Juliana.
Abstract Germline mutations in the TP53 gene are associated with Li-Fraumeni and Li-Fraumeni-Like Syndromes, characterized by increased predisposition to early-onset cancers. In Brazil, the prevalence of the TP53-p.R337H germline mutation is exceedingly high in the general population and in cancer-affected patients, probably as result of a founder effect. Several genotyping methods are used for the molecular diagnosis of LFS/LFL, however Sanger sequencing is still considered the gold standard. We compared performance, cost and turnaround time of Sanger sequencing, PCR-RFLP, TaqMan-PCR and HRM in the p.R337H genotyping. The performance was determined by analysis of 95 genomic DNA samples and results were 100% concordant for all methods. Sequencing was the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TP53-p.R337H; RFLP; TaqMan; HRM; Sanger Sequencing.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200203

Genetic diversity of tyrosine hydroxylase (TH) and dopamine b-hydroxylase (DBH) genes in cattle breeds
Provedor de dados: Genetics and Molecular Biology
Autores: Lourenco-Jaramillo,Diana Lelidett; Sifuentes-Rincón,Ana María; Parra-Bracamonte,Gaspar Manuel; Rosa-Reyna,Xochitl Fabiola de la; Segura-Cabrera,Aldo; Arellano-Vera,Williams.
DNA from four cattle breeds was used to re-sequence all of the exons and 56% of the introns of the bovine tyrosine hydroxylase (TH) gene and 97% and 13% of the bovine dopamine b-hydroxylase (DBH) coding and non-coding sequences, respectively. Two novel single nucleotide polymorphisms (SNPs) and a microsatellite motif were found in the TH sequences. The DBH sequences contained 62 nucleotide changes, including eight non-synonymous SNPs (nsSNPs) that are of particular interest because they may alter protein function and therefore affect the phenotype. These DBH nsSNPs resulted in amino acid substitutions that were predicted to destabilize the protein structure. Six SNPs (one from TH and five from DBH non-synonymous SNPs) were genotyped in 140 animals; all of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Behavior; B. t. indicus; B. t. taurus; Catecholamine; Temperament.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300009

Science and evolution
Provedor de dados: Genetics and Molecular Biology
Autores: Russo,Claudia A.M.; André,Thiago.
Abstract Evolution is both a fact and a theory. Evolution is widely observable in laboratory and natural populations as they change over time. The fact that we need annual flu vaccines is one example of observable evolution. At the same time, evolutionary theory explains more than observations, as the succession on the fossil record. Hence, evolution is also the scientific theory that embodies biology, including all organisms and their characteristics. In this paper, we emphasize why evolution is the most important theory in biology. Evolution explains every biological detail, similar to how history explains many aspects of a current political situation. Only evolution explains the patterns observed in the fossil record. Examples include the succession in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Evolutionary theory; Science; Scientific method; Scientific theory; Macroevolution.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100120

Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers
Provedor de dados: Genetics and Molecular Biology
Autores: Zucchi,Maria Imaculada; Brondani,Rosana Pereira Vianello; Pinheiro,José Baldin; Chaves,Lázaro José; Coelho,Alexandre Siqueira Guedes; Vencovsky,Roland.
The ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; Myrtaceae; Cerrado; Tropical tree; Genetic diversity and transferability.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400008

Use of the TSPY gene for sexing cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Lemos,Daniela Cristina; Rios,Álvaro Fabrício Lopes; Caetano,Lisandra Cristina; Lôbo,Raysildo Barbosa; Vila,Reginaldo Aparecido; Martelli,Lúcia; Takeuchi,Paula Lumy; Ramos,Ester Silveira.
The Y-encoded, testis-specific protein (TSPY) is a Y-specific gene. The copy numbers of TSPY range from 20 to 60 in men and up to 200 in bulls. In this study, we examined the possibility of using the TSPY gene to sex cattle. DNA from blood samples of 100 Nelore cattle (50 males and 50 females) from the Nelore Cattle Breeding Program (PMGRN) was screened for TSPY by PCR using TSPY-specific primers. The assay was highly specific since all male samples were TSPY-positive and all female samples were negative. Positive results were also obtained at low DNA concentrations (less than 1 rhog/muL). These results showed that TSPY was a good male-specific marker, the usefulness of which was enhanced by the high copy number of the gene. This is the first report to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Cattle; PCR; Sexing; TSPY.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100020

Relationship between XPD, RAD51, and APEX1 DNA repair genotypes and prostate cancer risk in the male population of Rio de Janeiro, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Cypriano,Ana Sheila; Alves,Gilda; Ornellas,Antonio Augusto; Scheinkman,José; Almeida,Renata; Scherrer,Luciano; Lage,Claudia.
Abstract Susceptibility to cancer ensues in individuals carrying malfunctioning DNA repair mechanisms. The impact of Single Nucleotide Polymorphisms (SNPs) in key DNA repair mechanisms on risk for prostate cancer was investigated in this case-control study. Samples consisted of 110 patients with confirmed prostate cancer and 200 unaffected men, from Rio de Janeiro, Brazil. XPD/Lys751Gln (rs13181), APEX1/Asp148Glu (rs1130409), and RAD51/G135C (rs1801320) SNPs were analyzed by PCR-RFLP. Allelic and genotypic frequencies were calculated and compared by Chi-Square test. The association between SNPs and clinical/epidemiological data was considered significant by Odds Ratio analysis, with IC95% and a p-value≤0.05. Only the XPD/Lys751Gln SNP significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; Single nucleotide polymorphism; XPD; DNA repair; Gene-environment interaction.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500751

In situ DNA transfer to chicken embryos by biolistics
Provedor de dados: Genetics and Molecular Biology
Autores: Ribeiro,Luciana A.; Azevedo,João L.; Aragão,Francisco J.L.; Rech,Elibio L.; Schmidt,Gilberto S.; Coutinho,Luiz L.
Fertilized chicken eggs were bombarded with a biolistic device. Transient expression of the lacZ gene under the control of a human cytomegalovirus (CMV) promoter was assessed after in situ gene transfer using this approach. The influence of different pressures, vacuum levels and particles was tested. Survival rate improved as particle velocity decreased, but resulted in lower levels of expression. The best survival and expression were obtained with gold particles, a helium gas pressure of 600 psi and a vacuum of 600 mmHg. Under these conditions, all bombarded embryos showed &lt;FONT FACE="Symbol"&gt;b&lt;/FONT&gt;-galactosidase activity, indicating that this was an effective method for transformation of chicken embryos.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400010

Frequency of the allelic variant c.1150T &gt; C in exon 10 of the fibroblast growth factor receptor 3 (FGFR3) gene is not increased in patients with pathogenic mutations and related chondrodysplasia phenotypes
Provedor de dados: Genetics and Molecular Biology
Autores: Kanazawa,Thatiane Yoshie; Bonadia,Luciana Cardoso; Cavalcanti,Denise Pontes.
Mutations in the FGFR3 gene cause the phenotypic spectrum of FGFR3 chondrodysplasias ranging from lethal forms to the milder phenotype seen in hypochondroplasia (Hch). The p.N540K mutation in the FGFR3 gene occurs in ~70% of individuals with Hch, and nearly 30% of individuals with the Hch phenotype have no mutations in the FGFR3, which suggests genetic heterogeneity. The identification of a severe case of Hch associated with the typical mutation c.1620C &gt; A and the occurrence of a c.1150T &gt; C change that resulted in a p.F384L in exon 10, together with the suspicion that this second change could be a modulator of the phenotype, prompted us to investigate this hypothesis in a cohort of patients. An analysis of 48 patients with FGFR3 chondrodysplasia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FGFR3; F384L; Hypochondroplasia; Skeletal dysplasia.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500003

Molecular forms of butyrylcholinesterase and obesity
Provedor de dados: Genetics and Molecular Biology
Autores: Boberg,Dellyana R.; Furtado-Alle,Lupe; Souza,Ricardo L.R.; Chautard-Freire-Maia,Eleidi A.
This study compared obese (N = 134) and unobese (N = 92) male blood donors, regarding the relative intensity (RI) and activity of different molecular forms (G1, G2, G4 and G1-ALB) of butyrylcholinesterase (BChE, EC 3.1.1.8) found in plasma, thereby searching for an association between these variables with obesity and SNPs of exons 1 and 4 of the BCHE gene. It was shown that obese and unobese individuals do not differ in the RI of each BChE band, even when classifying the sample into three genotypes of exons 1 and 4 of the BCHE gene (-116GG/539AA, -116GG/539AT, -116GA/539AT). Although the mean BChE activity of each band was significantly higher in obese than in unobese blood donors, the proportions of BChE bands were maintained, even under the metabolic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Butyrylcholinesterase; Body mass index; Obesity; BCHE gene SNPs.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300011

LEI0258 microsatellite variability and its relationship to B-F haplotypes in Brazilian (blue-egg Caipira) chickens
Provedor de dados: Genetics and Molecular Biology
Autores: Lima-Rosa,Carlos André da Veiga; Canal,Cláudio Wageck; Fallavena,Paulo Roberto Vargas; Freitas,Loreta Brandão de; Salzano,Francisco Mauro.
A total of 149 chickens from two different sources (one non-commercial, the other commercial) was tested for variability of the LEI0258 microsatellite. Fifty- three genotypes, explainable by 15 alleles, were found. There are clear allele and heterozygosity differences between the two samples. One of them was simultaneously studied for the MHC B-F haplotypes. Strong genetic disequilibrium was observed between the variants of the two systems, therefore providing a cheap alternative for MHC genotyping.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Microsatellite; LEI0258; B-F haplotypes; Brazilian chickens.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300008

Nuclear DNA content determination in Characiformes fish (Teleostei, Ostariophysi) from the Neotropical region
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Margarida Lima; Oliveira,Claudio; Navarrete,Maria Clara; Froehlich,Otávio; Foresti,Fausto.
In the present study, nuclear DNA content was analyzed in 53 species of Characiformes fish from the Neotropical region. Diploid number ranged from 2n = 48 in Astyanax fasciatus, Gymnocorymbus ternetzi and Hyphessobrycon griemi to 2n = 102 in Potamorhina squamoralevis, with a modal number of 54 chromosomes. Nuclear DNA content ranged from 1.70 ± 0.04 pg of DNA per diploid nucleus in Acestrorhynchus pantaneiro to 3.94 ± 0.09 pg in Tetragonopterus chalceus. A general analysis showed a mean value of 2.9 pg of DNA per diploid nucleus. Very similar DNA content values were observed in the species of the family Cynodontidae which showed a variation of 3% between the two genera studied. Small variations were observed between populations of Gymnocorymbus ternetzi,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nuclear DNA; DNA content; Chromosome; Fish; Evolution.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100010

Association between Knops blood group polymorphisms and susceptibility to malaria in an endemic area of the Brazilian Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Fontes,Aparecida Maria; Kashima,Simone; Bonfim-Silva,Ricardo; Azevedo,Rochele; Abraham,Kuruvilla Joseph; Albuquerque,Sérgio Roberto Lopes; Bordin,José Orlando; Langhi Júnior,Dante Mário; Covas,Dimas Tadeu.
Complement receptor 1 (CR1) gene polymorphisms that are associated with Knops blood group antigens may influence the binding of Plasmodium parasites to erythrocytes, thereby affecting susceptibility to malaria. The aim of this study was to evaluate the genotype and allele and haplotype frequencies of single-nucleotide polymorphisms (SNPs) of Knops blood group antigens and examine their association with susceptibility to malaria in an endemic area of Brazil. One hundred and twenty-six individuals from the Brazilian Amazon were studied. The CR1-genomic fragment was amplified by PCR and six SNPs and haplotypes were identified after DNA sequence analysis. Allele and haplotype frequencies revealed that the Kn b allele and H8 haplotype were possibly associated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian Amazon population; CR1 haplotypes; Knops blood group polymorphism; Malaria.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400002

Polymorphism in the internal transcribed spacer (ITS) of the ribosomal DNA of 26 isolates of ectomycorrhizal fungi
Provedor de dados: Genetics and Molecular Biology
Autores: Gomes,Eliane A.; Kasuya,Maria Catarina M.; Barros,Everaldo G. de; Borges,Arnaldo C.; Araújo,Elza F.
Inter- and intraspecific variation among 26 isolates of ectomycorrhizal fungi belonging to 8 genera and 19 species were evaluated by analysis of the internal transcribed sequence (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplification products, which ranged between 560 and 750 base pairs (bp), were obtained for all the isolates analyzed. The degree of polymorphism observed did not allow proper identification of most of the isolates. Cleavage of amplified fragments with the restriction enzymes Alu I, Hae III, Hinf I, and Hpa II revealed extensive polymorphism. All eight genera...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA fingerprinting; Ectomycorrhizal fungi; Internal transcribed spacer (ITS); PCR-RFLP; Ribosomal DNA (rDNA).
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400018

A comprehensive transcriptome analysis of skeletal muscles in two Polish pig breeds differing in fat and meat quality traits
Provedor de dados: Genetics and Molecular Biology
Autores: Piórkowska,Katarzyna; Żukowski,Kacper; Ropka-Molik,Katarzyna; Tyra,Mirosław; Gurgul,Artur.
Abstract Pork is the most popular meat in the world. Unfortunately, the selection pressure focused on high meat content led to a reduction in pork quality. The present study used RNA-seq technology to identify metabolic process genes related to pork quality traits and fat deposition. Differentially expressed genes (DEGs) were identified between pigs of Pulawska and Polish Landrace breeds for two the most important muscles (semimembranosus and longissimus dorsi). A total of 71 significant DEGs were reported: 15 for longissimus dorsi and 56 for semimembranosus muscles. The genes overexpressed in Pulawska pigs were involved in lipid metabolism (APOD, LXRA, LIPE, AP2B1, ENSSSCG00000028753 and OAS2) and proteolysis (CST6, CTSD, ISG15 and UCHL1). In Polish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA-seq; Firmness; Fat content; Polish pigs.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100125

Can Lutjanus purpureus (South red snapper) be "legally" considered a red snapper (Lutjanus campechanus)?
Provedor de dados: Genetics and Molecular Biology
Autores: Gomes,Grazielle; Schneider,Horacio; Vallinoto,Marcelo; Santos,Simoni; Orti,Guillermo; Sampaio,Iracilda.
Red snappers (Lutjanus purpureus in Brazil and Lutjanus campechanus in USA and Gulf of Mexico) are both under clear effect of overfishing. Because of their high morphological similarity it has already been suggested that they could possibly be considered as a single species. To investigate the degree of similarity and the genetic structure of red snapper populations we constructed a common dataset of partial D-loop mtDNA sequences of L. purpureus from Brazil (Amapá, Pará and Maranhão) and L. campechanus from the Atlantic coast of the USA (Florida, Louisiana and Mississippi). Phylogenetic and population genetic analyses surprisingly depicted high similarity between L. campechanus and L. purpureus, compatible with the hypothesis of a single species of red...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Red snapper; Lutjanus purpureus; Lutjanus campechanus; Population genetic structure; Mitochondrial D-loop.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200035

ADP-ribosylation: from molecular mechanisms to human disease
Provedor de dados: Genetics and Molecular Biology
Autores: Hoch,Nicolas C.; Polo,Luis M.
Abstract Post-translational modification of proteins by ADP-ribosylation, catalysed by poly (ADP-ribose) polymerases (PARPs) using NAD+ as a substrate, plays central roles in DNA damage signalling and repair, modulates a range of cellular signalling cascades and initiates programmed cell death by parthanatos. Here, we present mechanistic aspects of ADP-ribose modification, PARP activation and the cellular functions of ADP-ribose signalling, and discuss how this knowledge is uncovering therapeutic avenues for the treatment of increasingly prevalent human diseases such as cancer, ischaemic damage and neurodegeneration.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ADP ribose; PARP; DNA damage response; DNA repair; Parthanatos.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200304

Gel mobility shift scanning of pectin-inducible promoter from Penicillium griseoroseum reveals the involvement of a CCAAT element in the expression of a polygalacturonase gene
Provedor de dados: Genetics and Molecular Biology
Autores: Ribon,Andréa de O.B.; Ribeiro,João Batista; Gonçalves,Daniel B.; Queiroz,Marisa V. de; Araújo,Elza F. de.
Previous reports have described pgg2, a polygalacturonase-encoding gene of Penicillium griseoroseum, as an attractive model for transcriptional regulation studies, due to its high expression throughout several in vitro growth conditions, even in the presence of non-inducing sugars such as sucrose. A search for regulatory motifs in the 5' upstream regulatory sequence of pgg2 identified a putative CCAAT box that could justify this expression profile. This element, located 270 bp upstream of the translational start codon, was tested as binding target for regulatory proteins. Analysis of a 170 bp promoter fragment by electrophoretic mobility shift assay (EMSA) with nuclear extracts prepared from mycelia grown in pectin-containing culture medium revealed a high...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Penicillium griseoroseum; Polygalacturonase; 5' upstream regulatory sequence; Electrophoretic mobility shift assay.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100019

Evaluation of genetic damage induced by glyphosate isopropylamine salt using Tradescantia bioassays
Provedor de dados: Genetics and Molecular Biology
Autores: Alvarez-Moya,Carlos; Reynoso Silva,Mónica; Villalobos Arámbula,Alma Rosa; Islas Sandoval,Alfonso; Castañeda Vasquez,Hugo; González Montes,Rosa María.
Glyphosate is noted for being non-toxic in fishes, birds and mammals (including humans). Nevertheless, the degree of genotoxicity is seriously controversial. In this work, various concentrations of a glyphosate isopropylamine salt were tested using two methods of genotoxicity assaying, viz., the pink mutation assay with Tradescantia (4430) and the comet assay with nuclei from staminal cells of the same plant. Staminal nuclei were studied in two different forms, namely nuclei from exposed plants, and nuclei exposed directly. Using the pink mutation assay, isopropylamine induced a total or partial loss of color in staminal cells, a fundamental criterion utilized in this test. Consequently, its use is not recommended when studying genotoxicity with agents...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glyphosate; DNA damage; Comet assay; Plant genotoxicity test; Tradescantia point mutation test.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100022

Luiz Edmundo de Magalhães (1927-2012)
Provedor de dados: Genetics and Molecular Biology
Autores: Sene,Fábio de M.
Tipo: Info:eu-repo/semantics/report
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400001

Polymorphisms in promoter sequences of MDM2, p53, and p16INK4a genes in normal Japanese individuals
Provedor de dados: Genetics and Molecular Biology
Autores: Ohsaka,Yasuhito; Nishino,Hoyoku.
Research has been conducted to identify sequence polymorphisms of gene promoter regions in patients and control subjects, including normal individuals, and to determine the influence of these polymorphisms on transcriptional regulation in cells that express wild-type or mutant p53. In this study we isolated genomic DNA from whole blood of healthy Japanese individuals and sequenced the promoter regions of the MDM2, p53, and p16INK4a genes. We identified polymorphisms comprising 3 nucleotide substitutions at exon 1 and intron 1 regions of the MDM2 gene and 1 nucleotide insertion at a poly(C) nucleotide position in the p53 gene. The Japanese individuals also exhibited p16INK4a polymorphisms at several positions, including position -191. Reporter gene analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Murine double minute 2; Polymorphism; P16INK4a; P53; Transcription.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400004

Africanized honey bees (Apis mellifera L.) are more efficient at removing worker brood artificially infested with the parasitic mite Varroa jacobsoni Oudemans than are Italian bees or Italian/Africanized hybrids
Provedor de dados: Genetics and Molecular Biology
Autores: Guerra Jr.,José Carlos Vieira; Gonçalves,Lionel Segui; Jong,David De.
Africanized honey bees are more tolerant of infestations with the mite Varroa jacobsoni than are honey bees of European origin. The capacity of these bees to detect and react to brood infested with this mite could be one of the factors determining this tolerance. We tested colonies of Africanized bees headed by queens from swarms collected in Ribeirão Preto, São Paulo State. The Italian colonies had queens imported directly from the USA, or from the Brazilian Island of Fernando de Noronha, where varroa-infested Italian colonies have been maintained, untreated, since 1984. Recently sealed worker brood cells were artificially infested by opening the cell capping, inserting live adult female mites and resealing the cells. Control cells were treated in the...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100016

Chromosomal characterization of three native and one cultivated species of Lathyrus L. in Southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Battistin,Alice; Biondo,Elaine; Coelho,Liliana Gressler May.
Mitotic metaphase chromosomes and interphase nuclei of nine populations of three South American species of Lathyrus (L. pubescens, L. nervosus and L. crassipes) and six populations of the cultivated species L. odoratus were analyzed. All populations had 2n = 2x = 14 chromosomes. There were significant differences among populations within each species and among species in the number of metacentric, submetacentric and subtelocentric chromosomes, the number and location of secondary constrictions, chromosome length (longest and shortest), total haploid complement, arm ratio, and centromeric index. L. odoratus showed the highest tendency towards karyotype symmetry whereas the three South American species showed a moderate tendency towards asymmetry, with L....
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400016

A.N. Kolmogorov's defence of Mendelism
Provedor de dados: Genetics and Molecular Biology
Autores: Stark,Alan; Seneta,Eugene.
In 1939 N.I. Ermolaeva published the results of an experiment which repeated parts of Mendel's classical experiments. On the basis of her experiment she concluded that Mendel's principle that self-pollination of hybrid plants gave rise to segregation proportions 3:1 was false. The great probability theorist A.N. Kolmogorov reviewed Ermolaeva's data using a test, now referred to as Kolmogorov's, or Kolmogorov-Smirnov, test, which he had proposed in 1933. He found, contrary to Ermolaeva, that her results clearly confirmed Mendel's principle. This paper shows that there were methodological flaws in Kolmogorov's statistical analysis and presents a substantially adjusted approach, which confirms his conclusions. Some historical commentary on the Lysenko-era...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mendel's peas; Hybrids; Segregation ratio; Kolmogorov-Smirnov test; Chi-squared test.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200002

Phenotypic plasticity in colonizing populations of Drosophila subobscura
Provedor de dados: Genetics and Molecular Biology
Autores: Pegueroles,Glòria; Mestres,Francesc; Argemí,Mercè; Serra,Lluís.
The phenotypic plasticity of some quantitative traits of two colonizing populations of Drosophila subobscura (Davis and Eureka, California) was studied. Temperature effects and the effect of rearing in the laboratory were studied. Laboratory rearing during four generations at 18ºC significantly increased the wing and tibial length. This increase was similar to that obtained when the flies were reared at 13ºC during two generations.The low temperature environment can be considered more stressful for females than for males, as shown by the increase of phenotypic variance. The two populations analyzed had great phenotypic plasticity in spite of the genetic bottleneck during the colonization event. Our study shows that keeping flies for a relatively short time...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400008

In vivo evaluation of the antimutagenic and antigenotoxic effects of β-glucan extracted from Saccharomyces cerevisiae in acute treatment with multiple doses
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Rodrigo Juliano; Salles,Maria José Sparça; Silva,Ariane Fernanda da; Kanno,Tatiane Yumi Nakamura; Lourenço,Ana Carolina dos Santos; Leite,Véssia da Silva; Matiazi,Hevenilton José; Pesarini,João Renato; Ribeiro,Lúcia Regina; Mantovani,Mário Sérgio.
Ample evidence suggests that cancer is triggered by mutagenic damage and diets or supplements capable of reducing such incidences can be related to the prevention of neoplasy development or to an improvement in life quality of patients who undergo chemotherapy. This research aimed to evaluate the antimutagenic and antigenotoxic activity of β-glucan. We set up 8 experimental groups: control (Group 1), cyclophosphamide (Group 2), Groups 3-5 to assess the effect of β-glucan administration, and Groups 6-8 to evaluate the association between cyclophosphamide and β-glucan. The intraperitonial concentrations of β-glucan used were 100, 150 and 200 mg/kg. Micronucleus and comet assays showed that within the first week of treatment β-glucan presented a damage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-glucan; Cyclophosphamide; Antimutagenicity; Antigenotoxicity; Mice.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300017

Genetic differentiation of Macrodon ancylodon (Sciaenidae, Perciformes) populations in Atlantic coastal waters of South America as revealed by mtDNA analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Simôni; Schneider,Horacio; Sampaio,Iracilda.
The king weakfish (pescada-gó in Portuguese - Macrodon ancylodon (Sciaenidae), a demersal (bottom-feeding) species found in South America Atlantic coastal waters from the Gulf of Paria in Venezuela to Baia Blanca in Argentina, is an economically important species because of its abundance and wide acceptance by consumers. Because of its wide distribution this fish may be subject to geographic isolation and this may have resulted in distinct populations along its coastal range. Considering that this species represents an important economic resource, confirmation of whether M. ancylodon is a single species or there are different genetic stocks spread over its wide distribution would be an important contribution to conservation policies and population...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macrodon ancylodon; Sciaenidae; Perciformes; 16S rRNA; Cytochrome b; Genetic differentiation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200008

Identification and taxonomy of some entomopathogenic Paecilomyces spp. (Ascomycota) isolates using rDNA-ITS Sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Inglis,Peter W.; Tigano,Myrian S.
A phylogenetic analysis of the 5.8S rDNA and internal transcribed spacer (ITS1 and ITS2) sequences from some entomogenous Paecilomyces species supports the polyphyly of the genus and showed the existence of cryptic species. In the Eurotiales, anamorphs Paecilomyces variotii and Paecilomyces leycettanus were related to the teleomorphs Talaromyces and Thermoascus. In the Hypocreales, three major ITS subgroups were found, one of which included Paecilomyces viridis, Paecilomyces penicillatus, Paecilomyces carneus and isolates identified as Paecilomyces lilacinus and Paecilomyces marquandii. However, the majority of the P. lilacinus and P. marquandii isolates formed a distinct and distantly related subgroup, while the other major subgroup contained Paecilomyces...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paecilomyces spp.; Phylogenetic analysis; RDNA; Molecular taxonomy.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100025

Polymorphism of the catechol-O-methyltransferase gene   in Han Chinese patients with psoriasis vulgaris
Provedor de dados: Genetics and Molecular Biology
Autores: Gao,Lin; Gao,Lin; Li,Hong; Li,Hong; Li,Kai; Li,Kai; Shen,Zhu; Liu,Ling; Li,Chunying; Zhang,Zhengdong; Liu,Yufeng.
Psoriasis vulgaris is defined by a series of linked cellular changes in the skin: hyperplasia of epidermal keratinocytes, vascular hyperplasia and ectasia, and infiltration of T lymphocytes, neutrophils and other types of leukocytes in the affected skin. Catechol-O-methyltransferase ( COMT )  158  polymorphism can reduce the activity of the COMT enzyme that may trigger defective differentiation of keratinocytes in psoriasis. Immunocytes can degrade and inactivate catecholamines via monamine oxidase (MAO) and COMT in the cells. We hypothesized that the  COMT-158  G  &gt;  A  polymorphism was associated with the risk of psoriasis vulgaris in Han Chinese people. In a hospital-based case-control study, 524 patients with psoriasis vulgaris and 549...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COMT; Gene polymorphism; Genetic susceptibility; Psoriasis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100005

Identification and in silico expression pattern analysis of Eucalyptus expressed sequencing tags (ESTs) encoding molecular chaperones
Provedor de dados: Genetics and Molecular Biology
Autores: Cagliari,Thiago C.; Tiroli,Ana O.; Borges,Júlio C.; Ramos,Carlos H.I.
Expressed Sequence Tags (ESTs) sequencing provides reliable and useful information concerning gene expression patterns in the genomic context. Our group used bioinformatics to identify and annotate 5'EST-contigs belonging to the molecular chaperones within the Eucalyptus Genome Sequencing Project Consortium (FORESTs) database. We found that 1,959 5'EST-contigs, or approximately 1.6% of the total 5'EST-contigs, encoded chaperones, emphasizing their biological importance. About 55% of the chaperones that we found were Hsp70 chaperones and its co-chaperones, 18% were Hsp90 chaperones, 15% were Hsp60 and its co-chaperone, 8% were Hsp100 chaperones, and 4% were Small Hsps. We also investigated the digital expression profile of the chaperone genes to gain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chaperones; Heat shock proteins; Genome; Expressed sequence tags.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400006

The lactase persistence genotype is a protective factor for the metabolic syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Friedrich,Deise C.; Andrade,Fabiana M de; Fiegenbaum,Marilu; Almeida,Silvana de; Mattevi,Vanessa S.; Callegari-Jacques,Sidia M.; Hutz,Mara H.
The Metabolic Syndrome (MetS) is defined as a pattern of metabolic disturbances, which include central obesity, insulin resistance and hyperglycemia, dyslipidemia, and hypertension. Milk has been promoted as a healthy beverage that can improve the management of MetS. Most human adults, however, down-regulate the production of intestinal lactase after weaning. Lactase encoded by the LCT gene is necessary for lactose digestion. The -13910C &gt; T SNP (rs4988235) is responsible for the lactase persistence phenotype in European populations. We herein investigated whether the lactase persistence genotype is also associated with the MetS in subjects from a Brazilian population of European descent. This study consisted of 334 individuals (average age of 41 years)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic syndrome; Lactase persistence; Hypolactasia; Lactose.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500001

Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients
Provedor de dados: Genetics and Molecular Biology
Autores: Del Valle,Paulo Roberto; Milani,Cintia; Brentani,Maria Mitzi; Katayama,Maria Lucia Hirata; Lyra,Eduardo Carneiro de; Carraro,Dirce Maria; Brentani,Helena; Puga,Renato; Lima,Leandro A.; Rozenchan,Patricia Bortman; Nunes,Bárbara dos Santos; Góes,João Carlos Guedes Sampaio; Folgueira,Maria Aparecida Azevedo Koike.
Cancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene expression profile of stromal cells from primary tumor (CAF, n = 4), lymph node metastasis (N+, n = 3) and bone marrow (BM, n = 4) obtained from breast cancer patients. Biological validation was done in another 16 samples by RT-qPCR. Differences between CAF vs N+, CAF vs BM and N+ vs BM were represented by 20, 235 and 245 genes, respectively (SAM test, FDR &lt; 0.01). Functional analysis revealed that genes related to development and morphogenesis were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow; Breast neoplasm; Fibroblast; Gene expression profiling; Lymph node; Mesenchymal stromal cells; Tumor microenvironment.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400002

Different levels of hsp70 and hsc70 mRNA expression in Iberian fish exposed to distinct river conditions
Provedor de dados: Genetics and Molecular Biology
Autores: Jesus,Tiago F.; Inácio,Ângela; Coelho,Maria M.
Comprehension of the mechanisms by which ectotherms, such as fish, respond to thermal stress is paramount for understanding the threats that environmental changes may pose to wild populations. Heat shock proteins are molecular chaperones with an important role in several stress conditions such as high temperatures. In the Iberian Peninsula, particularly in Portugal, freshwater fish of the genus Squalius are subject to daily and seasonal temperature variations. To examine the extent to which different thermal regimes influence the expression patterns of hsp70 and hsc70 transcripts we exposed two species of Squalius (S. torgalensis and S. carolitertii) to different temperatures (20, 25, 30 and 35 ºC). At 35 ºC, there was a significant increase in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyprinidae; Heat shock proteins; Squalius; Thermal stress.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100009

Patterns of genetic diversity in southern and southeastern Araucaria angustifolia (Bert.) O. Kuntze relict populations
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Maria Isabel Ferreira de; Salgueiro,Fabiano; Carnavale-Bottino,Mariana; Félix,Durvalina Benedita; Alves-Ferreira,Marcio; Bittencourt,Juliana Vitoria Messias; Margis,Rogério.
Habitat fragmentation and a decrease in population size may lead to a loss in population genetic diversity. For the first time, the reduction in genetic diversity in the northernmost limit of natural occurence (southeastern Brazil) of Araucaria angustifolia in comparison with populations in the main area of the species continuous natural distribution (southern Brazil), was tested. The 673 AFLPs markers revealed a high level of genetic diversity for the species (Ht = 0.27), despite anthropogenic influence throughout the last century, and a decrease of H in isolated populations of southeastern Brazil (H = 0.16), thereby indicating the tendency for higher genetic diversity in remnant populations of continuous forests in southern Brazil, when compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucariaceae; Relict populations; Araucaria forest; Population genetics; AFLP.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300018

Evidence of hybridization between two species of Melipona bees
Provedor de dados: Genetics and Molecular Biology
Autores: Nascimento,Vania Alves; Matusita,Syomara Hakiko; Kerr,Warwick Estevam.
This report describes a case of hybridization between two species of Meliponinae bees (Melipona scutellaris from the Diamantina plateau in the State of Bahia, and Melipona capixaba from around Domingos Martins, in the State of Espírito Santo, Brazil). To demonstrate hybridization, electrophoretic profiles of esterase activity from three colonies were studied. Ten adult workers of M. scutellaris, M. capixaba and the hybrid colony were collected and processed individually. The pattern of esterase activity was constant for each species but differed between them, whereas hybrid bees had a pattern derived from both species. The fact that two ecologically different species of stingless bees separated by more than 300 km could still cross when placed in the same...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100014

Evaluation of DNA damage in an Ecuadorian population exposed to glyphosate
Provedor de dados: Genetics and Molecular Biology
Autores: Paz-y-Miño,César; Sánchez,María Eugenia; Arévalo,Melissa; Muñoz,María José; Witte,Tania; De-la-Carrera,Gabriela Oleas; Leone,Paola E.
We analyzed the consequences of aerial spraying with glyphosate added to a surfactant solution in the northern part of Ecuador. A total of 24 exposed and 21 unexposed control individuals were investigated using the comet assay. The results showed a higher degree of DNA damage in the exposed group (comet length = 35.5 µm) compared to the control group (comet length = 25.94 µm). These results suggest that in the formulation used during aerial spraying glyphosate had a genotoxic effect on the exposed individuals.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; DNA damage; Ecuador; Genotoxicity; Glyphosate.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300026

Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale
Provedor de dados: Genetics and Molecular Biology
Autores: Nieddu,Mariella; Mezzanotte,Roberto; Pichiri,Giuseppina; Coni,Pier Paolo; Dedola,Gian Luca; Dettori,Maria Luisa; Pazzola,Michele; Vacca,Giuseppe Massimo; Robledo,Renato.
Abstract Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experiments showed completely coherent results: the Bovini probe Bt1 hybridized only to members of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1 hybridized only to members of the Caprini tribe and not to members of Bovini. Hybridization signals were detected in the heterochromatic regions of every acrocentric autosome, except for two pairs of autosomes from Capra hircus that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovidae; Satellite DNA; Fluorescence in situ hybridization; Pericentromeric region.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400513

Heterologous microsatellite primer pairs informative for the whole genus Arachis
Provedor de dados: Genetics and Molecular Biology
Autores: Hoshino,Andrea Akemi; Bravo,Juliana Pereira; Angelici,Carla M.L.C.D.; Barbosa,Andréa V. Gobbi; Lopes,Catalina Romero; Gimenes,Marcos A.
The genus Arachis currently comprises 69 described species, some of which have potential and real value as human and animal foods. These Arachis species have been collected and maintained in germplasm banks to provide material that can be used as sources of genes in breeding programs and for the selection of new cultivars. One of the principal objectives of germplasm conservation is the evaluation of genetic variability, which is best conducted using molecular markers. We investigated the use of heterologous primers to amplify microsatellite loci that could be used to evaluate genetic variability in Arachis germplasm. Fifteen microsatellite primer pairs were tested in 76 accessions of 34 species from the nine Arachis sections. The data indicated that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peanut; Genetic relationship; Molecular markers; Wild relatives.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400016

Frequency of the Q192R and L55M polymorphisms of the human serum paraoxonase gene (PON1) in ten Amazonian Amerindian tribes
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Ney Pereira Carneiro dos; Ribeiro-dos-Santos,Ândrea KC; Santos,Sidney EB.
Human serum paraoxonase (PON1) is an esterase associated with high density lipoproteins (HDLs) in the plasma and may confer protection against coronary artery disease. Serum PON1 levels and activity vary widely among individuals and populations of different ethnic groups, such variations appearing to be related to two coding region polymorphisms (L55M and Q192R). Several independent studies have indicated that the polymorphism at codon 192 (the R form) is a significant risk factor for cardiovascular disease in some populations, although this association has not been confirmed in other populations. Given the possible associations of these mutations with heart diseases and the fact that little or nothing is known of their prevalence in Amerindian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human serum paraoxonase; Populations genetics; Amerindian; PON1; Amazonian populations.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100006

Genetic variability in common wheat germplasm based on coefficients of parentage
Provedor de dados: Genetics and Molecular Biology
Autores: Bered,Fernanda; Barbosa-Neto,José F.; Carvalho,Fernando I.F. de.
The characterization of genetic variability and an estimate of the genetic relationship among varieties are essential to any breeding program, because artificial crosses among less similar parents allow a larger segregation and the combination of different favorable alleles. Genetic variability can be evaluated in different ways, including the Coefficient of Parentage (COP), which estimates the probability of two alleles in two different individuals being identical by descent. In this study, we evaluated the degree of genetic relationship among 53 wheat genotypes, and identified the ancestor genotypes which contributed the most to the current wheat germplasm, as a prediction of the width of the genetic base of this cereal. The results revealed a mean COP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COP; Genetic base; Gene pool; Triticum aestivum L.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200015

Meiotic chromosomes and nucleolar behavior in testicular cells of the grassland spittlebugs Deois flavopicta, Mahanarva fimbriolata and Notozulia entreriana (Hemiptera, Auchenorrhyncha)
Provedor de dados: Genetics and Molecular Biology
Autores: Castanhole,Márcia Maria Urbanin; Pereira,Luis Lênin Vicente; Souza,Hederson Vinicius de; Valério,José Raul; Barbosa,Leonardo Rodrigues; Itoyama,Mary Massumi.
Spittlebugs annually infest pastures and cause severe damage, representing a serious problem for the tropical American beef cattle industry. Spittlebugs are an important biotic constraint to forage production and there is a lack of cytogenetic data for this group of insects. For these reasons, we conducted this work, in which the spermatogenesis and nucleolar behavior of Deois flavopicta, Mahanarva fimbriolata and Notozulia entreriana were studied. The males possessed testes in the shape of a "bunch of grapes"; a variable number of testicular lobes per individual and polyploid nuclei composed of several heteropycnotic bodies. A heteropycnotic area was located in the periphery of the nucleus (prophase I); the chiasmata were terminal or interstitial;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal characterization; Karyotype; Cytogenetics; Spermatogenesis; Holocentric chromosomes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200008

Diversity and evolution of plant diacylglycerol acyltransferase (DGATs) unveiled by phylogenetic, gene structure and expression analyses
Provedor de dados: Genetics and Molecular Biology
Autores: Turchetto-Zolet,Andreia Carina; Christoff,Ana Paula; Kulcheski,Franceli Rodrigues; Loss-Morais,Guilherme; Margis,Rogerio; Margis-Pinheiro,Marcia.
Abstract Since the first diacylglycerol acyltransferase (DGAT) gene was characterized in plants, a number of studies have focused on understanding the role of DGAT activity in plant triacylglycerol (TAG) biosynthesis. DGAT enzyme is essential in controlling TAGs synthesis and is encoded by different genes. DGAT1 and DGAT2 are the two major types of DGATs and have been well characterized in many plants. On the other hand, the DGAT3 and WS/DGAT have received less attention. In this study, we present the first general view of the presence of putative DGAT3 and WS/DGAT in several plant species and report on the diversity and evolution of these genes and its relationships with the two main DGAT genes (DGAT1 and DGAT2). According to our analyses DGAT1, DGAT2,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triacylglycerol biosynthesis; DGAT; Phylogeny; Gene structure.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400524

Expression profile of oxidative and antioxidative stress enzymes based on ESTs approach of citrus
Provedor de dados: Genetics and Molecular Biology
Autores: Peroni,Luis Antonio; Ferreira,Renato Rodrigues; Figueira,Antonio; Machado,Marcos Antonio; Stach-Machado,Dagmar Ruth.
Plants not only evolve but also reduce oxygen in photosynthesis. An inevitable consequence of this normal process is the production of reactive oxygen species (ROS). Plants are adequately protected by the presence of multiple antioxidative enzymes in the cytosol and also in the different cell organelles such as chloroplasts, mitochondria, and peroxisomes. Traditionally, ROS were considered to be only a toxic byproduct of aerobic metabolism. However, recently it has become apparent that plants actively produce these molecules which may control many different physiological processes such as abiotic and biotic stress response, pathogen defense and systemic signaling. The search results using the Citrus Genome Program in Brazil (CitEST) for oxidative stress...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Genome; EST; Reactive oxygen species; Oxidative stress enzyme.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500016

Genetic analysis of the infestation of females of the Caracu cattle breed by Horn Fly (Haematobia irritans irritans) (L.) (Diptera, Muscidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Fraga,Angelina Bossi; Alencar,Maurício Mello de; Figueiredo,Leopoldo Andrade de; Razook,Alexander George; Cyrillo,Joslaine Noely dos Santos Gonçalves.
The objective of this study was to evaluate environmental factors that affect infestation of Caracu cattle breed by horn fly (Haematobia irritans) and to estimate genetic parameters for level of infestation. Infestation was evaluated on females from two herds in two consecutive years. Total number of flies on animals (FC) was counted, an infestation score (FS: 1 to 5) was recorded according to the estimated number of flies on animals, and number of parasites in photographs (FF) taken when the animals were evaluated was also counted. On each animal from one to eight observations were taken. A total of 3,836, 2,751 and 3,754 records from 718 animals were obtained for FC, FF and FS, respectively. The incidence of flies was lower during winter and higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic correlation; Heritability; Horn fly counts; Repeatability.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200011

Nuclear DNA content and karyotype of Rosewood (Aniba rosaeodora)
Provedor de dados: Genetics and Molecular Biology
Autores: Contim,Luis Antônio Serrão; Carvalho,Carlos Roberto de; Martins,Franciele Alline; Freitas,Danival Vieira de.
Rosewood (Aniba rosaeodora Ducke, Lauraceae) is ecologically and economically important to the Amazon region. As a consequence of its economic importance, rosewood populations have been decimated in the Amazon forest. Species of nine genera of the Lauraceae family have characterized karyotypes with n = x = 12 chromosomes in the gametophytic phase but the genus Aniba is one of the least studied Lauraceae genera with a previously undescribed genome. We used cytogenetic techniques to determine that the A. rosaeodora karyotype contained 12 pairs (2n = 24) of relatively small submetacentric chromosomes with lengths ranging from 1.34 to 2.25 mum and a nucleolar organizer region (NOR) in the short arm of chromosome 7. Flow cytometry gave 2C = 2.32 pg of DNA,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosewood; Aniba rosaeodora; Lauraceae; Karyotype; Flow cytometry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500017

GenFlow: generic flow for integration, management and analysis of molecular biology data
Provedor de dados: Genetics and Molecular Biology
Autores: Oikawa,Marcio Katsumi; Broinizi,Marcos Eduardo Bolelli; Dermargos,Alexandre; Armelin,Hugo Aguirre; Ferreira,João Eduardo.
A large number of DNA sequencing projects all over the world have yielded a fantastic amount of data, whose analysis is, currently, a big challenge for computational biology. The limiting step in this task is the integration of large volumes of data stored in highly heterogeneous repositories of genomic and cDNA sequences, as well as gene expression results. Solving this problem requires automated analytical tools to optimize operations and efficiently generate knowledge. This paper presents an information flow model , called GenFlow, that can tackle this analytical task.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological workflow; Information modeling; Semantic and control data; Molecular sequence analysis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400035

Sequence and expression pattern of the germ line marker vasa in honey bees and stingless bees
Provedor de dados: Genetics and Molecular Biology
Autores: Tanaka,Érica Donato; Hartfelder,Klaus.
Queens and workers of social insects differ in the rates of egg laying. Using genomic information we determined the sequence of vasa, a highly conserved gene specific to the germ line of metazoans, for the honey bee and four stingless bees. The vasa sequence of social bees differed from that of other insects in two motifs. By RT-PCR we confirmed the germ line specificity of Amvasa expression in honey bees. In situ hybridization on ovarioles showed that Amvasa is expressed throughout the germarium, except for the transition zone beneath the terminal filament. A diffuse vasa signal was also seen in terminal filaments suggesting the presence of germ line cells. Oocytes showed elevated levels of Amvasa transcripts in the lower germarium and after follicles...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apis mellifera; Melipona quadrifasciata; Vasa; Social insect; Oogenesis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300023

Methods for detection of horizontal transfer of transposable elements in complete genomes
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Marcos Oliveira de; Loreto,Elgion L.S.
Recent advances in nucleic acid sequencing technology are creating a diverse landscape for the analysis of horizontal transfer in complete genomes. Previously limited to prokaryotes, the availability of complete genomes from close eukaryotic species presents an opportunity to validate hypotheses about the patterns of evolution and mechanisms that drive horizontal transfer. Many of those methods can be transported from methods previously used in prokaryotic genomes, as the assumptions for horizontal transfer can be interpreted as the same. Some methods, however, require a complete adaptation, while others need refinements in sensitivity and specificity to deal with the huge datasets generated from next-generation sequencing technologies. Here we list the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transposable element; Horizontal transfer; Genome; Computational analysis; Evolution.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600024

Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
Provedor de dados: Genetics and Molecular Biology
Autores: Otero,Luize; Cavalcanti Júnior,Geraldo Barroso; Klumb,Claudete Esteves; Scheiner,Marcos Antonio Mauricio; Magluta,Eliane Pereira Simões; Fernandez,Teresa de Souza; Silva,Maria Luiza Macedo; Pires,Virgínia; Andrade,Gabriela Vasconcelos; Maia,Raquel Ciuvalschi; Tabak,Daniel.
chronic myeloid leukemia (CML) have been described. This chromosomal region contains the tumor suppressor gene TP53 that may be an important factor in the evolution of this disease. In this study, we used flow cytometry and western blotting to assess p53 protein expression and single stranded conformational polymorphism to examine TP53 gene alterations in three patients with CML who showed alterations in 17p. Only the case with del(17)(p11) had p53 expression positive by flow cytometry and an abnormal migration pattern by SSCP analysis. The importance of the correlation between the results obtained with these techniques, as well as the clinical course of the patients, are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic myeloid leukemia; Chromosome 17; TP53 gene; P53 protein.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100007

William D. Hamilton e a evolução do comportamento social e do altruísmo
Provedor de dados: Genetics and Molecular Biology
Autores: Kerr,Warwick Estevam.
Tipo: Info:eu-repo/semantics/report
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200044

Cancer-related worry and risk perception in Brazilian individuals seeking genetic counseling for hereditary breast cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Palmero,Edenir Inêz; Campacci,Natalia; Schüler-Faccini,Lavinia; Giugliani,Roberto; Rocha,José Claudio Casali da; Vargas,Fernando Regla; Ashton-Prolla,Patricia.
Abstract In Brazil, the population in general has little knowledge about genetic risks, as well as regarding the role and importance of the Cancer Genetic Counseling (CGC). The goal of this study was to evaluate cancer-related worry and cancer risk perception during CGC sessions in Brazilian women at-risk for hereditary breast cancer. This study was performed in 264 individuals seeking CGC for hereditary breast cancer. Both cancer-affected and unaffected individuals were included. As results, individuals with and without cancer reported different motivations for seeking CGC and undergoing genetic testing. A correlation was observed between age at the first CGC session and age at which the closest relative was diagnosed with cancer. Multivariate analysis...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hereditary breast cancer; Hereditary cancer; Cancer-related worry; Cancer risk perception; Genetic counselling.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400104

Prevalence and impact of founder mutations in hereditary breast cancer in Latin America
Provedor de dados: Genetics and Molecular Biology
Autores: Ashton-Prolla,Patricia; Vargas,Fernando Regla.
Approximately 10% of all cancers are considered hereditary and are primarily caused by germline, high penetrance mutations in cancer predisposition genes. Although most cancer predisposition genes are considered molecularly heterogeneous, displaying hundreds of different disease-causing sequence alterations, founder mutations have been identified in certain populations. In some Latin American countries, founder mutations associated with increased risk of breast and other cancers have been described. This is particularly interesting considering that in most of these countries, populations are highly admixed with genetic contributions from native populations and from the influx of several distinct populations of immigrants. In this article, we present a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer genes; BRCA1; BRCA2; TP53; Cancer predisposition.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200009

Underrated Staphylococcus species and their role in antimicrobial resistance spreading
Provedor de dados: Genetics and Molecular Biology
Autores: Rossi,Ciro César; Pereira,Monalessa Fábia; Giambiagi-deMarval,Marcia.
Abstract The increasing threat of antimicrobial resistance has shed light on the interconnection between humans, animals, the environment, and their roles in the exchange and spreading of resistance genes. In this review, we present evidences that show that Staphylococcus species, usually referred to as harmless or opportunistic pathogens, represent a threat to human and animal health for acting as reservoirs of antimicrobial resistance genes. The capacity of genetic exchange between isolates of different sources and species of the Staphylococcus genus is discussed with emphasis on mobile genetic elements, the contribution of biofilm formation, and evidences obtained either experimentally or through genome analyses. We also discuss the involvement of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horizontal gene transfer; Antimicrobial resistance; Coagulase negative staphylococcus; Biofilm; CRISPR; One Health.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000300303

Validation of the micronucleus-centromere assay for biological dosimetry
Provedor de dados: Genetics and Molecular Biology
Autores: Wojcik,A.; Kowalska,M.; Bouzyk,E.; Buraczewska,I.; Kobialko,G.; Jarocewicz,N.; Szumiel,I.
The micronucleus assay is frequently used for purposes of biological dosimetry. Due to high interindividual variability in the spontaneous frequency of micronuclei, its sensitivity in the low dose region is poor. It has been suggested that this problem can be mitigated by selectively analyzing the frequency of those micronuclei which contain only acentric fragments. Using a pan-centromeric FISH probe we have studied the dose dependence of micronuclei with centromeres in peripheral lymphocytes of human donors. In contrast to previous publications, our approach is based on determining the relative frequency of micronuclei with and without centromeric signals. Our results confirm previous observations that in the low dose range of ionizing radiation, the...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400055

Cystic fibrosis mutations R1162X and 2183AA®G in two southern Brasilian states
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Lilian; Raskin,Salmo; Freund,Aline A.; Ribas,Patrícia D.; Castro,Raquel M.V.; Pignatti,Pier F.; Culpi,Lodércio.
We screened 79 southern Brazilian patients with cystic fibrosis for the rare cystic fibrosis mutations R1162X and 2183AA&lt;FONT FACE="Symbol"&gt;®&lt;/FONT&gt;G. Forty-nine patients were born in the State of Paraná (PR) and 30 in the State of Santa Catarina (SC). Two 2183AA&lt;FONT FACE="Symbol"&gt;®&lt;/FONT&gt;G alleles were found among the SC patients and one among the PR patients. Six R1162X alleles were found among the SC patients and one among the PR patients. Fourteen percent of the alleles found among patients of Italian origin were R1162X, and 7% were 2183AA&lt;FONT FACE="Symbol"&gt;®&lt;/FONT&gt;G mutations. These mutations, together with &lt;FONT FACE="Symbol"&gt;D&lt;/FONT&gt;F508, were also studied in a sample of 270 normal non-related...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300002

Comparison of similarity coefficients based on RAPD markers in the common bean
Provedor de dados: Genetics and Molecular Biology
Autores: Duarte,Jair Moura; Santos,João Bosco dos; Melo,Leonardo Cunha.
The alterations caused by eight different similarity coefficients were evaluated in the clustering and ordination of 27 common bean (Phaseolus vulgaris L.) cultivars analyzed by RAPD markers. The Anderberg, simple matching, Rogers and Tanimoto, Russel and Rao, Ochiai, Jaccard, Sorensen-Dice, and Ochiai II's coefficients were tested. Comparisons among the coefficients were made through correlation analysis of genetic distances obtained by the complement of these coefficients, dendrogram evaluation (visual inspection and consensus fork index - CIC), projection efficiency in a two-dimensional space, and groups formed by Tocher's optimization procedure. The employment of different similarity coefficients caused few alterations in cultivar classification, since...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300024

Prevalence of Café-au-Lait Spots in children with solid tumors
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Anna Claudia Evangelista dos; Heck,Benjamin; Camargo,Beatriz De; Vargas,Fernando Regla.
Abstract Cafe-au-lait maculae (CALM) are frequently observed in humans, and usually are present as a solitary spot. Multiple CALMs are present in a smaller fraction of the population and are usually associated with other congenital anomalies as part of many syndromes. Most of these syndromes carry an increased risk of cancer development. Previous studies have indicated that minor congenital anomalies may be more prevalent in children with cancer. We investigated the prevalence of CALMs in two samples of Brazilian patients with childhood solid tumors, totaling 307 individuals. Additionally, 176 school children without diagnosis of cancer, or of a cancer predisposing syndrome, were investigated for the presence of CALMs. The prevalence of solitary CALM was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Café-au-lait maculae; Pediatric solid tumors; Birth defects; Nosology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200232

Ribosome binding site recognition using neural networks
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Márcio Ferreira da Silva; Mendes,Daniele Quintella; Ferrari,Luciana Itida; Vasconcelos,Ana Tereza Ribeiro.
Pattern recognition is an important process for gene localization in genomes. The ribosome binding sites are signals that can help in the identification of a gene. It is difficult to find these signals in the genome through conventional methods because they are highly degenerated. Artificial Neural Networks is the approach used in this work to address this problem.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics; Neural networks; Ribosome binding site.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400028

Israel Roisenberg (1934-2013): a life dedicated to science
Provedor de dados: Genetics and Molecular Biology
Autores: Salzano,Francisco M.
Tipo: Info:eu-repo/semantics/article
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100001

Medical Genetics – Special issue dedicated to the 35th anniversary of the Medical Genetics Service, Hospital de Clínicas de Porto Alegre, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Giugliani,Roberto.
Tipo: Info:eu-repo/semantics/other
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200153

Developmental genetic analysis of fruit shape traits under different environmental conditions in sponge gourd (Luffa cylindrical (L) Roem. Violales, Cucurbitaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Zhang,Sheng; Hu,Jin; Xu,Shengchun.
Analysis of genetic main effects and genotype × environment (GE) interaction effects for the fruit shape traits fruit length and fruit circumference in the sponge gourd (Luffa cylindrical (L) Roem. Violales, Cucurbitaceae) was conducted for diallel cross data from two planting seasons. A genetic model including fruit direct effects and maternal effects and unconditional and conditional variances analysis was used to evaluate the development of the fruit at four maturation stages. The variance analysis results indicated that fruit length and circumference were simultaneously affected by fruit direct genetic effects and maternal effects as well as GE interaction effects. Fruit direct genetic effects were relatively more important for both fruit shape traits...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fruit shape traits; Genetic analysis; Genetic variances; Luffa cylindrical (L) Roem.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400017

Silencing of the integrin-linked kinase gene suppresses the proliferation, migration and invasion of pancreatic cancer cells (Panc-1)
Provedor de dados: Genetics and Molecular Biology
Autores: Zhu,Xiang-Yu; Liu,Ning; Liu,Wei; Song,Shao-Wei; Guo,Ke-Jian.
Integrin-linked kinase (ILK) is an ankyrin repeat-containing serine-threonine protein kinase that is involved in the regulation of integrin-mediated processes such as cancer cell proliferation, migration and invasion. In this study, we examined the effect of a lentivirus-mediated knockdown of ILK on the proliferation, migration and invasion of pancreatic cancer (Panc-1) cells. Immunohistochemical staining showed that ILK expression was enhanced in pancreatic cancer tissue. The silencing of ILK in human Panc-1 cells led to cell cycle arrest in the G0/G1 phase and delayed cell proliferation, in addition to down-regulating cell migration and invasion. The latter effects were mediated by up-regulating the expression of E-cadherin, a key protein in cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: E-cadherin; Epithelial-mesenchymal transition (EMT); Integrin-linked kinase (ILK); Panc-1 cell line; RNA interference (RNAi).
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300022

On extending the Hardy-Weinberg law
Provedor de dados: Genetics and Molecular Biology
Autores: Stark,Alan E.
This paper gives a general mating system for an autosomal locus with two alleles. The population reproduces in discrete and non-overlapping generations. The parental population, the same in both sexes, is arbitrary as is that of the offspring and the gene frequencies of the parents are maintained in the offspring. The system encompasses a number of special cases including the random mating model of Weinberg and Hardy. Thus it demonstrates, in the most general way possible, how genetic variation can be conserved in an indefinitely large population without invoking random mating or balancing selection. An important feature is that it provides a mating system which identifies when mating does and does not produce Hardy-Weinberg proportions among offspring.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hardy-Weinberg law; Non-random mating; General offspring distribution.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400026

Fragile X founder effect and distribution of CGG repeats among the mentally retarded population of Andalusia, South Spain
Provedor de dados: Genetics and Molecular Biology
Autores: Diego,Yolanda de; Hmadcha,Abdelkrim; Moron,Francisco; Lucas,Miguel; Carrasco,Mercedes; Pintado,Elizabeth.
Fragile X syndrome is the most common inherited form of mental retardation. We investigated the prevalence of the Fragile X syndrome in the population with mental retardation of unknown etiology in Andalusia, South Spain. We analyzed 322 unrelated patients (280 males and 42 females), and found a fragile X syndrome frequency of 6.5%. Among the non-fragile X chromosomes, the 29 CGG repeat was the most common allele. At the linked microsatellite DXS548 locus, we found a new allele which we called "allele 10" (17 CA). Similar to other south European populations, allele 2 (25 CA) at the DXS548 locus and the fragile X allele were in linkage disequilibrium supporting the idea of a common founder chromosome predisposing to the CGG expansion.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mental retardation; Fragile X syndrome; CGG repeats; Genetic screening.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100002

How history and geography may explain the distribution in the Comorian archipelago of a novel mutation in DNA repair-deficient xeroderma pigmentosum patients
Provedor de dados: Genetics and Molecular Biology
Autores: Sarasin,Alain; Munier,Patrick; Cartault,François.
Abstract Xeroderma pigmentosum (XP) is a rare, genetic, autosomal nucleotide excision repair-deficient disease characterized by sun-sensitivity and early appearance of skin and ocular tumors. Thirty-two black-skinned XP from Comoros, located in the Indian Ocean, were counted, rendering this area the highest world prevalence of XP. These patients exhibited a new homozygous XPC mutation at the 3’-end of the intron12 (IVS 12-1G&gt;C) leading to the absence of XPC protein. This mutation, characteristic of the consanguineous Comorian families, is associated with a founder effect with an estimated age of about 800 years. Analysis of mt-DNA and Y-chromosome identified the haplogroups of patients, who are derived from the Bantu people. Although the four Comorian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA repair deficient diseases; Sun-sensitivity; Comorian Archipelago; Bantu haplogroups; XPC gene.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200305

Distribution of the CCR2-64I allele in three Brazilian ethnic groups
Provedor de dados: Genetics and Molecular Biology
Autores: Acosta,Angelina Xavier; Sampaio,Rogério Grimaldi; Spínola,Juliana Lima; Galvão-Castro,Bernardo.
CCR2 is a member of the superfamily of seven transmembrane domain G protein-coupled receptors, the largest receptor superfamily in the human genome. CCR2 acts as a receptor for MCP-1 (CC chemokine) and as a co-receptor for HIV-1 cell-target entry. The gene encoding this receptor is mapped to the chromosome band 3p21. A G-to-A transition at position 190 characterizes the CCR2-64I mutation, causing valine to isoleucine substitution in codon 64. This mutation has been identified as an important factor for delaying progression to AIDS. Here, we determined the prevalence of this allele in three different Brazilian populations: 261 Amerindians inhabiting an isolated region in northern Brazil (82 samples from the Waiampi tribe, and 179 samples from the Tiriyó...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CCR2-64I mutation; Brazilian populations; HIV co-receptor.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300004

MLL leukemia-associated rearrangements in peripheral blood lymphocytes from healthy individuals
Provedor de dados: Genetics and Molecular Biology
Autores: Brassesco,María Sol; Montaldi,Ana Paula; Gras,Diana Ester; Queiroz,Rosane Gomes de Paula; Martinez-Rossi,Nilce Maria; Tone,Luiz Gonzaga; Sakamoto-Hojo,Elza Tiemi.
Chromosomal translocations are characteristic of hematopoietic neoplasias and can lead to unregulated oncogene expression or the fusion of genes to yield novel functions. In recent years, different lymphoma/leukemia-associated rearrangements have been detected in healthy individuals. In this study, we used inverse PCR to screen peripheral lymphocytes from 100 healthy individuals for the presence of MLL (Mixed Lineage Leukemia) translocations. Forty-nine percent of the probands showed MLL rearrangements. Sequence analysis showed that these rearrangements were specific for MLL translocations that corresponded to t(4;11)(q21;q23) (66%) and t(9;11) (20%). However, RT-PCR failed to detect any expression of t(4;11)(q21;q23) in our population. We suggest that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genomic instability; Lymphocytes; MLL rearrangements.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200005

Polymorphisms in the glutathione S-transferase theta and mu genes and susceptibility to myeloid leukemia in Brazilian patients
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Claudio Lima; Barbosa,Cynara Gomes; Moura Neto,José Pereira de; Barreto,José Henrique; Reis,Mitermayer Galvão; Gonçalves,Marilda Souza.
The null genotype for glutathione S-transferase (GST, EC 2.5.1.18) gene polymorphisms is considered a risk factor for leukemia in different populations. In this work we investigated the GSTT1 and GSTM1 polymorphisms using multiplex PCR in 53 patients with chronic myeloid leukemia (CML), 23 with acute promyelocytic leukemia (APL) and 304 apparently healthy controls. In this association study we found that the GSTT1null genotype was more frequent in our group of APL patients than in the control group [OR = 2.75 (95% CI = 1.10-6.88)], providing evidence that a deletion in the GSTT1 gene could be a risk factor for this type of leukemia.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Acute promyelocytic leukemia; Chronic myeloid leukemia; GSTM1; GSTT1; Gene polymorphism.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100008

Agmatoploidy and symploidy: a critical review
Provedor de dados: Genetics and Molecular Biology
Autores: Guerra,Marcelo.
Abstract Agmatoploidy is a type of chromosome rearrangement that involves the fragmentation of an entire chromosome complement, generating a diploid with double its original chromosome number. Agmatoploidy and other related karyotype changes, such as symploidy (the opposite change, promoted by chromosome fusion), partial agmatoploidy, polyagmatoploidy, etc., are restricted to species with holokinetic chromosomes and are assumed to play an important role in their karyotype evolution. However, a critical review of the literature shows that examples of chromosome number doubling by agmatoploidy are rare and not clearly demonstrated, while partial agmatoploidy and partial symploidy seem to be the same as ascending and descending disploidy, respectively. It is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agmatoploidy; Symploidy; Holocentrics; Holokinetic chromosomes; Chromosome evolution.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400492

Karyotype and genome size comparative analyses among six species of the oilseed-bearing genus Jatropha (Euphorbiaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Marinho,Anne C.T.A.; Vasconcelos,Santelmo; Vasconcelos,Emanuelle V.; Marques,Daniela A.; Benko-Iseppon,Ana Maria; Brasileiro-Vidal,Ana Christina.
Abstract Jatropha is an important genus of Euphorbiaceae, with species largely used for various purposes, including the manufacturing of soaps and pharmaceutical products and applications in the bioenergetic industry. Although there have been several studies focusing J. curcas in various aspects, the karyotype features of Jatropha species are poorly known. Therefore, we analyzed six Jatropha species through fluorochrome staining (CMA/DAPI), fluorescent in situ hybridization (FISH) with 5S and 45S rDNA probes and genome size estimation by flow cytometry. Our results revealed several chromosome markers by both CMA/DAPI and FISH for the analyzed species. Five Jatropha species (J. curcas, J. gossypiifolia, J. integerrima, J. multifida and J. podagrica) showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotaxonomy; DNA C-value; Heterochromatin; Physic nut; RDNA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300442

Quantitative trait locus affecting birth weight on bovine chromosome 5 in a F2 Gyr x Holstein population
Provedor de dados: Genetics and Molecular Biology
Autores: Gasparin,Gustavo; Miyata,Marcelo; Coutinho,Luiz Lehmann; Martinez,Mário Luiz; Silva,Marcos Vinícius G. Barbosa da; Machado,Marco Antônio; Campos,Ana Lúcia; Regitano,Luciana Correia de Almeida.
Segregation between a genetic marker and a locus influencing a quantitative trait in a well delineated population is the basis for success in mapping quantitative trait loci (QTL). To detect bovine chromosome 5 (BTA5) birth weight QTL we genotyped 294 F2 Gyr (Bos indicus) x Holstein (Bos taurus) crossbreed cattle for five microsatellite markers. A linkage map was constructed for the markers and an interval analysis for the presence of QTL was performed. The linkage map indicated differences in the order of two markers relative to the reference map (&lt;A HREF="http://www.marc.usda.gov/"&gt;http://www.marc.usda.gov&lt;/A&gt;). Interval analysis detected a QTL controlling birth weight (p &lt; 0.01) at 69 centimorgans (cM) from the most centromeric marker...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BTA5; Birth weight; Cattle; QTL; Microsatellite markers.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500005

D-MaPs - DNA-microarray projects: web-based software for multi-platform microarray analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Carazzolle,Marcelo F.; Herig,Taís S.; Deckmann,Ana C.; Pereira,Gonçalo A.G.
The web application D-Maps provides a user-friendly interface to researchers performing studies based on microarrays. The program was developed to manage and process one- or two-color microarray data obtained from several platforms (currently, GeneTAC, ScanArray, CodeLink, NimbleGen and Affymetrix). Despite the availability of many algorithms and many software programs designed to perform microarray analysis on the internet, these usually require sophisticated knowledge of mathematics, statistics and computation. D-maps was developed to overcome the requirement of high performance computers or programming experience. D-Maps performs raw data processing, normalization and statistical analysis, allowing access to the analyzed data in text or graphical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microarray; Web service; Software; Affymetrix and nimblegen.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300030

Genetic control of plant architecture in the common bean (Phaseolus vulgaris L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Teixeira,Flavia França; Ramalho,Magno Antonio Patto; Abreu,Ângela de Fátima Barbosa.
More erect plant architecture has been a goal in the development of bean cultivars. Aiming to obtain more information about genetic control of traits related to plant architecture, this work was carried out between August 1995 and July 1997 in the southern and Alto São Francisco regions, in the State of Minas Gerais, Brazil. Initially, analyses were performed with individual plants of parents and different segregant generations from the crosses Carioca-MG x H-4 and Carioca x FT-Tarumã. In these experiments, besides degree of erectness, other traits were evaluated: ramification degree, internode length, internode diameter and height of insertion of the first pod. Mean and variance components and heritability at an individual level were estimated. Later,...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400019

Genetic structure of Brazilian wild rice (Oryza glumaepatula Steud., Poaceae) populations analyzed using microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Karasawa,Marines M.G.; Vencovsky,Roland; Silva,Cynthia M.; Zucchi,Maria Imaculada; Oliveira,Giancarlo C.X.; Veasey,Elizabeth A.
Knowledge of the genetic structure and diversity of natural populations is important in developing strategies for in situ and ex situ conservation. We used eight microsatellite loci to estimate genetic structure and investigate within and between population genetic variation in eleven Brazilian wild rice (Oryza glumaepatula) populations. The study showed the following genetic diversity parameters: average number of 3.1 alleles per locus; 77.3% polymorphic loci; 0.091 observed heterozygosity and 0.393 gene diversity. F-statistics detected by microsatellite loci were: F ST = 0.491 (and R ST = 0.608), F IS = 0.780 and F IT = 0.888. No population was in Hardy-Weinberg equilibrium. The estimated apparent outcrossing rate (0.143) indicated a predominance of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Gene flow; Genetic diversity; Microsatellites; Oryza glumaepatula.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300017

Unraveling the evolutionary scenario of the hobo element in populations of Drosophila melanogaster and D. simulans in South America using the TPE repeats as markers
Provedor de dados: Genetics and Molecular Biology
Autores: Ragagnin,Geovani T.; Bernardo,Larissa P.; Loreto,Elgion L. S.
Abstract Transposable elements (TEs) are nucleotide sequences found in most studied genomes. These elements are highly diversified and have a large variation in nucleotide structure and mechanisms of transposition. hobo is a member of class II, belonging to hAT superfamily, described inDrosophila melanogaster, and it presents in its Open Reading Frame, a repetitive region encoding the amino acids threonine-proline-glutamic acid (TPE), which shows variability in the number of repeats in some regions of the world. Due to this variability some evolutionary scenarios of the hobo element are discussed, such as the scenario of the invasion of hobo element in populations ofD. melanogaster. In the present study, we investigated 22 DNA sequences of D. melanogaster...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Transposable elements; Transposons; HAT; Genomic evolution; Molecular evolution.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100145

Genetic diversity and phylogeny in Hystrix (Poaceae, Triticeae) and related genera inferred from Giemsa C-banded karyotypes
Provedor de dados: Genetics and Molecular Biology
Autores: Zhang,Hai-Qin; Yang,Rui-Wu; Zhang,Li; Ding,Chun-Bang; Zeng,Jian; Zhou,Yong-Hong.
The phylogenetic relationships of 15 taxa from Hystrix and the related genera Leymus (NsXm), Elymus (StH), Pseudoroegneria (St), Hordeum (H), Psathyrostachys (Ns), and Thinopyrum (E) were examined by using the Giemsa C-banded karyotype. The Hy. patula C-banding pattern was similar to those of Elymus species, whereas C-banding patterns of the other Hystrix species were similar to those of Leymus species. The results suggest high genetic diversity within Hystrix, and support treating Hy. patula as E. hystrix L., and transferring Hy. coreana, Hy. duthiei ssp. duthiei and Hy. duthiei ssp. longearistata to the genus Leymus. On comparing C-banding patterns of Elymus species with their diploid ancestors (Pseudoroegneria and Hordeum), there are indications that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-banding; Elymus; Genome; Hystrix; Leymus; Triticeae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300015

Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera)
Provedor de dados: Genetics and Molecular Biology
Autores: Tavares,Mara Garcia; Azeredo-Oliveira,Maria Tercilia Vilela de; Ceron,Carlos Roberto.
We examined the esterases present in the hemolymph and Malpighian tubules of "Kissing bug", Triatoma infestans (Triatominae, Heteroptera) by polyacrylamide gel electrophoresis. Six esterase bands were observed and were designated EST 1 to EST 6. EST 1, 4, 5 and 6 were exclusive to hemolymph, whereas EST 2 and 3 were found only in Malpighian tubules. Each tissue had a characteristic esterase pattern, which may be related to its functional role. The four hemolymph esterases hydrolyzed a-naphthyl acetate. One of these enzymes was classified as a carboxylesterase (EST 4), and another was an acetylesterase (EST 6). The other two enzymes (EST 1 and 5) could be either carboxylesterases or serino-proteases with an esterolytic function, as they were selectively...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400009

Transplacental inhibitory effect of carrot juice on the clastogenicity of cyclophosphamide in mice
Provedor de dados: Genetics and Molecular Biology
Autores: Gimmler-Luz,Maria Clara; Cardoso,Valesca Veiga; Sardiglia,Cassius Ugarte; Widholzer,Débora da Silva.
Genetic damage during the prenatal period can provoke important neoplastic alterations and other diseases in postnatal life. Beta-carotene (ßC) is considered to be one of the most important anticarcinogens in the diet and can protect mammalian cells against genotoxic events. As carrots are important dietary source of ßC, we decided to test the effect of fresh carrot juice (CaJ) on cyclophosphamide (CP)-induced genotoxicity in maternal and fetal erythropoietic tissues. The treatment with CaJ started on the 7th day of the pregnancy of BALB/c female mice. We observed, on the 16th gestational day, that this treatment did not modify the spontaneous frequency of micronucleated polychromatic erythrocytes (mPCE) in the bone marrow of the females nor in the livers...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100013

Biochemical diagnosis of mucopolysaccharidosis in a Mexican reference center
Provedor de dados: Genetics and Molecular Biology
Autores: Mendoza-Ruvalcaba,Sandra del Carmen; Brambila-Tapia,Aniel Jessica Leticia; Juárez-Osuna,Jesús Alejandro; Silva-José,Thiago Donizete Da; García-Ortiz,José Elías.
Abstract Mucopolysaccharidoses (MPS) are a group of genetic disorders, each resulting from the deficiency of one of the lysosomal enzymes that catabolizes mucopolysaccharides. For the accurate diagnosis of the disease, the quantification of a specific enzymatic activity is needed. In the present study, we analyzed seven MPS over several periods of time ranging from 2 to 5 years in a reference center in Mexico. During this time, a total of 761 samples belonging to 505 individuals with suspected MPS were analyzed. A total of 198 (26.01%) positive results were found. Among these, MPS IVA accounted for the highest frequency of positive results (49.10%), followed by MPS III (17.69%, IIIA: 11.80% and IIIB: 5.89%). Adjusting for the number of births per year, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucopolysaccharidoses; Leukocyte enzymatic activity; Fluorometric assay; Mexico.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100106

Frequency of 677C -&gt; T and 1298A -&gt; C polymorphisms in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene in Turner syndrome individuals
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Kelly; Lemos-Marini,Sofia H.V.; Baptista,Maria T.M.; Bonadia,Luciana Cardoso; Pinto Júnior,Walter; Bertuzzo,Carmen Sílvia.
Turner syndrome (TS) is an interesting model for investigating the association between methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and non-disjunction because of the high frequency of chromosomal mosaicism among patients with this syndrome. We determined the frequencies of MTHFR 677C -&gt; T and 1298A -&gt; C polymorphic mutations in 49 patients with TS and 200 control individuals. The frequency of the 677C -&gt; T allele was 0.39 for patients and 0.29 for controls while that of the 1298A -&gt; C allele was 0.28 for patients and 0.25 for controls. Genotype frequencies were shown to be different in patients and controls (chi2 = 12.143; p = 0.033), and this was attributable to the higher frequency of the C677C -&gt; T /677C -&gt; T...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal non-disjunction; MTHFR gene; Turner syndrome.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100008

Cultivable bacteria isolated from apple trees cultivated under different crop systems: Diversity and antagonistic activity against Colletotrichum gloeosporioides
Provedor de dados: Genetics and Molecular Biology
Autores: Passos,João Frederico M. dos; Costa,Pedro B. da; Costa,Murilo D.; Zaffari,Gilmar R.; Nava,Gilberto; Boneti,José Itamar; Oliveira,Andréia Mara R. de; Passaglia,Luciane M.P.
This study evaluated the diversity of cultivable plant growth-promoting (PGP) bacteria associated with apple trees cultivated under different crop management systems and their antagonistic ability against Colletotrichum gloeosporioides. Samples of roots and rhizospheric soil from apple trees cultivated in organic and conventional orchards in southern Brazil were collected, together with soil samples from an area never used for agriculture (native field). Bacteria were identified at the genus level by PCR-RFLP and partial sequencing of the 16S rRNA, and were evaluated for some PGP abilities. The most abundant bacterial genera identified were Enterobacter (27.7%), Pseudomonas (18.7%), Burkholderia (13.7%), and Rahnella (12.3%). Sixty-nine isolates presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant growth-promoting bacteria; Apple tree; Antagonistic activity; Colletotrichum gloeosporioides.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400013

Comparative linkage mapping of Oryza glumaepatula and Oryza sativa interspecific crosses based on microsatellite and expressed sequence tag markers
Provedor de dados: Genetics and Molecular Biology
Autores: Rangel,Priscila Nascimento; Brondani,Rosana Pereira Vianello; Coelho,Alexandre Siqueira Guedes; Rangel,Paulo Hideo Nakano; Brondani,Claudio.
Molecular linkage maps representing the rice genome have been an important tool for breeding programs because they allow the elucidation of polygenic traits and are an efficient tool for monitoring wild introgressions in interspecific crosses. Common markers among rice genetic maps are important in defining the homology of chromosomes and the synteny between genomic target regions. We used 148 markers (expressed sequence tags, microsatellites and single nucleotide polymorphisms) to construct a molecular linkage map based on co-dominant markers for an interspecific backcross population using a wild rice (Oryza glumaepatula) from Brazil and performed a comparative analysis with other interspecific maps. The comparative analysis revealed a Spearman...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comparative linkage map; Molecular markers; Oryza glumaepatula; Rice.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400019

Phenotype-oriented NGS panels for mucopolysaccharidoses: Validation and potential use in the diagnostic flowchart
Provedor de dados: Genetics and Molecular Biology
Autores: Brusius-Facchin,Ana Carolina; Siebert,Marina; Leão,Delva; Malaga,Diana Rojas; Pasqualim,Gabriela; Trapp,Franciele; Matte,Ursula; Giugliani,Roberto; Leistner-Segal,Sandra.
Abstract Mucopolysaccharidosis (MPS) are a group of rare genetic disorders caused by deficiency in the activity of specific lysosomal enzymes required for the degradation of glycosaminoglycans (GAGs). A defect in the activity of these enzymes will result in the abnormal accumulation of GAGs inside the lysosomes of most cells, inducing progressive cellular damage and multiple organ failure. DNA samples from 70 patients with biochemical diagnosis of different MPSs genotypes confirmed by Sanger sequencing were used to evaluate a Next Generation Sequencing (NGS) protocol. Eleven genes related to MPSs were divided into three different panels according to the clinical phenotype. This strategy led to the identification of several pathogenic mutations distributed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lysosomal storage disease; Mucopolysaccharidoses; Next generation sequencing; Target sequence; Mutation detection.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200207

Monosomy 7 in donor cell-derived leukemia after bone marrow transplantation for severe aplastic anemia: report of a new case and review of the literature
Provedor de dados: Genetics and Molecular Biology
Autores: Otero,Luize; Souza,Daiane Correa de; Tavares,Rita de Cássia; Gomes,Bernadete Evangelho; Padilha,Telma França; Bouzas,Luiz Fernando; Fernandez,Teresa de Souza; Abdelhay,Eliana.
Monosomy 7 arises as a recurrent chromosome aberration in donor cell leukemia after hematopoietic stem cell transplantation. We report a new case of donor cell leukemia with monosomy 7 following HLA-identical allogenic bone marrow transplantation for severe aplastic anemia (SAA). The male patient received a bone marrow graft from his sister, and monosomy 7 was detected only in the XX donor cells, 34 months after transplantation. The patient's bone marrow microenvironment may have played a role in the leukemic transformation of the donor hematopoietic cells.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monosomy 7; Donor cell leukemia; Bone marrow transplantation; Severe aplastic anemia.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500004

Genetic analysis of D-xylose metabolism by endophytic yeast strains of Rhodotorula graminis and Rhodotorula mucilaginosa
Provedor de dados: Genetics and Molecular Biology
Autores: Xu,Ping; Bura,Renata; Doty,Sharon L.
Two novel endophytic yeast strains, WP1 and PTD3, isolated from within the stems of poplar (Populus) trees, were genetically characterized with respect to their xylose metabolism genes. These two strains, belonging to the species Rhodotorula graminis and R. mucilaginosa, respectively, utilize both hexose and pentose sugars, including the common plant pentose sugar, D-xylose. The xylose reductase (XYL1) and xylitol dehydrogenase (XYL2) genes were cloned and characterized. The derived amino acid sequences of xylose reductase (XR) and xylose dehydrogenase (XDH) were 32%~41% homologous to those of Pichia stipitis and Candida. spp., two species known to utilize xylose. The derived XR and XDH sequences of WP1 and PTD3 had higher homology (73% and 69% identity)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhodotorula graminis; Rhodotorula mucilaginosa; D-xylose metabolism; Xylitol; Xylitol dehydrogenase; Xylose reductase.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300018

Chromosomal imbalances detected in primary bone tumors by comparative genomic hybridization and interphase fluorescence in situ hybridization
Provedor de dados: Genetics and Molecular Biology
Autores: Baruffi,Marcelo Razera; Engel,Edgard Edward; Squire,Jeremy Andrew; Tone,Luis Gonzaga; Rogatto,Silvia Regina.
We applied a combination of comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH), to characterize the genetic aberrations in three osteosarcomas (OS) and one Ewing's sarcoma. CGH identified recurrent chromosomal losses at 10p14-pter and gains at 8q22.3-24.1 in OS. Interphase FISH allowed to confirm 8q gain in two cases. A high amplification level of 11q12-qter was detected in one OS. The Ewing's sarcoma showed gain at 1p32-36.1 as the sole chromosome alteration. These studies demonstrate the value of molecular cytogenetic methods in the characterization of recurrent genomic alterations in bone tumor tissue.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comparative genomic hybridization; CGH; Osteosarcoma; Ewing's Sarcoma.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200001

Effects of sorghum (sorghum bicolor L.) root exudates on the cell cycle of the bean plant (phaseolus vulgaris L.) root
Provedor de dados: Genetics and Molecular Biology
Autores: Hallak,Angela Maria Gattás; Davide,Lisete Chamma; Souza,Itamar Ferreira.
Two experiments were conducted to test the allelopathic effect of sorghum (Sorghum bicolor L.) root exudates on bean (Phaseolus vulgaris L.) cell division. Research was conducted in the greenhouse of the Wistock Agricultural Research Institute of Minas Gerais State (EPAMIG) and in a laboratory of the Federal University of Lavras (UFLA). Sorghum variety BR-601 and bean variety Carioca MG were used. The exudate, called sorgoleone (SGL), was obtained by methylene chloride and acetic acid extraction from sorghum roots seven days after sowing on Petri dishes, and refrigerated until use. Solutions of 0.01, 0.05, 0.10, and 0.15 mM were prepared using Johanson solution as the SGL solvent. Seven-day-old bean seedlings grown in vermiculite in a greenhouse were...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100018

Morphological characterization and genetic analysis of Drechslera teres isolates
Provedor de dados: Genetics and Molecular Biology
Autores: Frazzon,A.P.G.; Matsumura,A.T.S.; Van Der Sand,S.T.
Net blotch, caused by the phytopathogen Drechslera teres, is a common disease of barley (Hordeum vulgare L) and is responsible for large economic losses in some barley growing areas. In this study the morphology and genetic variability of eight D. teres isolates from different regions of the Brazilian state of Rio Grande do Sul were investigated. Colony morphology was studied on potato-dextrose-agar (PDA) and genetic variability investigated using the random amplified polymorphic-DNA (RAPD) technique. 27 commercially available primers were tested of which 16 were selected for use in polymorphic analysis due to their good resolution and reproducibility. Similarity coefficients were used to construct dendrograms based on colony morphology and RAPD data...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drechslera teres; Barley; Net blotch; DNA polymorphism; RAPD.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200019

Identification of LincRNA from Dermatophagoides farinae (Acari: Pyroglyphidae) for Potential Allergen-Related Targets
Provedor de dados: Genetics and Molecular Biology
Autores: Zhou,Ying; Wu,Meili; Zhu,Hanting; Shao,Junjie; Liu,Chang; Cui,Yubao.
Abstract Long noncoding RNAs (lncRNAs), especially their important subclass of long intergenic noncoding RNAs (lincRNAs), have been identified in some insects. They play important roles in the regulation of biological processes, such as immune response or cell differentiation and as possible evolutionary precursors for protein coding genes. House dust mites (HDMs) are recognized as allergenic mites because allergens are found in their feces and bodies. Dermatophagoides farinae is one of the most important pyroglyphid mites because of its abundance in the household. To determine if lincRNAs can regulate allergen presentation in HDMs, we analyzed RNA-seq data for HDMs. We identified 11 lincRNAs that are related to mRNAs coding for allergens in HDMs. Using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: House dust mites (HDMs); Dermatophagoides farinae; Long noncoding RNAs (lncRNAs); Allergen; RNA-seq.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100804

First karyotypical description of two American Ciconiiform birds, Mycteria americana (Ciconiidae) and Platalea ajaja (Threskiornithidae) and its significance for the chromosome evolutionary and biological conservation approaches
Provedor de dados: Genetics and Molecular Biology
Autores: Francisco,Mercival Roberto; Galetti Junior,Pedro Manoel.
The karyotypes of Mycteria americana (Ciconiidae) and Platalea ajaja (Threskiornithidae) are described. Both species have a diploid number of 2n = 72. There are slight chromosome morphology differences, which could be related to distinct chromosome evolution pathways of these two families. Besides a better understanding of the chromosome relationships among Ciconiiformes, this first chromosome characterization of M. americana and P. ajaja is an important tool for the conservation of both species.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400015

Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes
Provedor de dados: Genetics and Molecular Biology
Autores: Marthe,Jefferson de Brito; Pompolo,Silvia das Graças; Campos,Lucio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.
Four colonies of the stingless bee Partamona cupira (Hymenoptera: Apidae) were cytogenetically analyzed using conventional staining and the fluorochromes CMA3 e DAPI. The females have 2n = 34 chromosomes (2K=32&lt;img border=0 src="../../../../../../../img/revistas/gmb/2010nahead/ah06a04CarM.gif" align=absmiddle&gt;+2&lt;img border=0 src="../../../../../../../img/revistas/gmb/2010nahead/ah06a04CarA.gif" align=absmiddle&gt;). Some females, however, presented an additional large B acrocentric chromosome, to a total of 2n = 35. Chromosome B and the chromosomal pairs 2, 9 and 10 showed CMA3+ bands, indicating an excess of CG base-pairs. A clear association was verified between the P. helleri B chromosome SCAR marker and the presence of a B chromosome in P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B chromosome; SCAR; Stingless bees.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200009

Occurrence of B chromosomes in Tetragonisca Latreille, 1811 (Hymenoptera, Apidae, Meliponini): a new contribution to the cytotaxonomy of the genus
Provedor de dados: Genetics and Molecular Biology
Autores: Barth,Adriane; Fernandes,Anderson; Pompolo,Silvia das Graças; Costa,Marco Antônio.
Tetragonisca angustula and Tetragonisca fiebrigi have recently been listed as valid species. This study aimed to cytogenetically investigate both species, emphasizing the new registry of B chromosomes in the tribe Meliponini. We analyzed colonies of T. angustula and T. fiebrigi collected at Tangará da Serra, Mato Grosso, Brazil, through conventional Giemsa staining, C-banding, and base-specific fluorochrome staining (CMA3/DAPI). T. angustula showed 2n = 34 chromosomes in females and n = 17 in males, with karyotype formula 2K = 34A M. T. fiebrigi showed numeric variation, with chromosome number varying from 2n = 34 to 2n = 36 in females and from n = 17 to n=18in males, with karyotype formula 2K = 32A M+2A Mc and 2K = 32A M+2A Mc + 1 or 2 B-chromosomes. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stingless bees; Cytogenetics; Interspecific differentiation; B chromosomes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100014

Challenges and prospects of population genetic studies in terns (Charadriiformes, Aves)
Provedor de dados: Genetics and Molecular Biology
Autores: Faria,Patrícia J.; Baus,Erika; Morgante,João S.; Bruford,Michael W.
Little information is available about the population structure of communally nesting terns (Sternidae) and skimmers (Rynchopidae) throughout the world. In order to fill this gap, a survey of molecular markers was carried out for six species of terns (Anous stolidus, Sterna hirundinacea, S. fuscata, S. superciliaris, Thalasseus maximus and Phaetusa simplex) and one species of skimmer (Rynchops niger). First, we describe the results of the construction of genomic DNA libraries and document problems encountered during this procedure. Secondly, we tested the cross-amplification of 18 microsatellite loci previously described for related species (the number of polymorphic loci ranged from three to seven). Thirdly, we tested the usefulness of mtDNA (control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Microsatellites; Mitochondrial DNA; Terns.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400029

Genetic variability and efficiency of DNA microsatellite markers for paternity testing in horse breeds from the Brazilian Marajó archipelago
Provedor de dados: Genetics and Molecular Biology
Autores: Reis,Sávio P.; Gonçalves,Evonnildo C.; Silva,Artur; Schneider,Maria P.C.
In this study, 15 microsatellite DNA loci used in comparative tests by the International Society for Animal Genetics were applied to the evaluation of genetic diversity and management, and the efficiency of paternity testing in Marajoara horses and Puruca ponies from the Marajó Archipelago. Based on the genotyping of 93 animals, mean allelic diversity was estimated as 9.14 and 7.00 for the Marajoara and Puruca breeds, respectively. While these values are similar to those recorded in most European breeds, mean levels of heterozygosity were much lower (Marajoara 49%, Puruca 40%), probably as a result of high levels of inbreeding in the Marajó populations. The mean informative polymorphic content of this 15-marker system was over 50% in both breeds, and was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Marajoara horses; Puruca ponies; Microsatellite markers; Genetic diversity; Geneaological control.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100014

Genetic characterization of the population of São Luís, MA, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Ferreira,Francileide Lisboa; Leal-Mesquita,Emygdia Rosa; Santos,Sidney Emanuel Batista dos; Ribeiro-dos-Santos,Ândrea Kely Campos.
Five loci (vWA1, F13A1, D12S67, Apo-B and D1S80) were investigated by polyacrylamide gel electrophoresis followed by silver staining in a sample of 177 individuals from the population of São Luís, State of Maranhão, Brazil. A total of 70 different alleles were identified. A statistically significant deviation from the Hardy-Weinberg equilibrium was observed in a single locus (F13A1, p = 0.0075). The average heterozygosity (H) was estimated at 77.7%, the mean number of alleles per locus as 14. The PD (capacity of genotype differentiation at each locus) ranged from 88.9% (vWA1) to 96.7% (F13A1). The combined PE (power of exclusion) of these five loci was 99.8%. In terms of racial admixture (42% European, 39% Indian, and 19% African Black ancestry), São Luís...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon region; DNA; Polymorphisms; VNTRs; STRs; Interethnic admixture.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100004

Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniae
Provedor de dados: Genetics and Molecular Biology
Autores: Pinto,Paulo Marcos; Carvalho,Marcos Oliveira de; Alves-Junior,Leonardo; Brocchi,Marcelo; Schrank,Irene Silveira.
Diversification of bacterial species and pathotypes is largely caused by lateral gene transfer (LGT) of diverse mobile DNA elements such as plasmids, phages, transposons and genomic islands. Thus, acquisition of new phenotypes by LGT is very important for bacterial evolution and relationship with hosts. This paper reports a 23 kb region containing fourteen CDSs with similarity to the previous described Integrative Conjugal Element of Mycoplasma fermentans (ICEF). This element, named ICEH, is present as one copy at distinct integration sites in the chromosome of 7448 and 232 pathogenic strains and is absent in the type strain J (non-pathogenic). Notable differences in the nucleotide composition of the insertion sites were detected, and could be correlated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LGT; Mycoplasma; Constins.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200014

Hordein variation in Brazilian barley varieties (Hordeum vulgare L.) and wild barley (H. euclaston Steud. and H. stenostachys Godr.)
Provedor de dados: Genetics and Molecular Biology
Autores: Echart-Almeida,Cinara; Cavalli-Molina,Suzana.
SDS-PAGE was used to analyze the hordein polypeptide patterns of Brazilian barley varieties (Hordeum vulgare L.) and of two native species of Hordeum from southern Brazil (H. euclaston Steud. and H. stenostachys Godr.). Forty different hordein polypeptide bands with molecular weights ranging from 30 to 94 kDa were found in the seeds of the three species studied. Twelve of the 14 varieties examined showed intravarietal polymorphism. The number of bands ranged from 10 to 17, depending on the variety, and from 3 to 13 among individual seeds, with a total of 26 bands in H. vulgare. Phenograms using each seed as an operational taxonomic unit (OTU) showed that the seeds from most varieties did not form distinct clusters. Seeds from different plants of the native...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200031

Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae)
Provedor de dados: Genetics and Molecular Biology
Autores: Rocha,Marília de França; Melo,Natoniel Franklin de; Souza,Maria José de.
The grasshopper species Orthoscapheus rufipes and Eujivarus fusiformis were analyzed using several cytogenetic techniques. The karyotype of O. rufipes, described here for the first time, had a diploid number of 2n = 23, whereas E. fusiformis had a karyotype with 2n = 21. The two species showed the same mechanism of sex determination (XO type) but differed in chromosome morphology. Pericentromeric blocks of constitutive heterochromatin (CH) were detected in the chromosome complement of both species. CMA3/DA/DAPI staining revealed CMA3-positive blocks in CH regions in four autosomal bivalents of O. rufipes and in two of E. fusiformis. The location of active NORs differed between the two species, occurring in bivalents M6 and S9 of O. rufipes and M6 and M7 of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Constitutive heterochromatin; Fluorochromes; FISH; Orthoptera; Ribosomal DNA.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200008

Conservation implications of the mating system of the Pampa Hermosa landrace of peach palm analyzed with microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Picanço-Rodrigues,Doriane; Astolfi-Filho,Spartaco; Lemes,Maristerra R.; Gribel,Rogerio; Sebbenn,Alexandre M.; Clement,Charles R.
Peach palm (Bactris gasipaes) is cultivated by many indigenous and traditional communities from Amazonia to Central America for its edible fruits, and is currently important for its heart-of-palm. The objective of this study was to investigate the mating system of peach palm, as this is important for conservation and breeding. Eight microsatellite loci were used to genotype 24 open-pollinated progenies from three populations of the Pampa Hermosa landrace maintained in a progeny trial for genetic improvement. Both the multi-locus outcrossing rates (0.95 to 0.99) and the progeny level multi-locus outcrossing rates (0.9 to 1.0) were high, indicating that peach palm is predominantly allogamous. The outcrossing rates among relatives were significantly different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bactris gasipaes; Bi-parental inbreeding; Genetic improvement; Genetic conservation; Outcrossing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100059

Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Carlos Henrique S.; Zehr,Usha B.; Gunaratna,Nilupa; Anderson,Joseph; Kononowicz,Halina H.; Hodges,Thomas K.; Axtell,John D.
The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agrobacterium tumefaciens; GUS; Transformation; Sorghum.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200022

Chromosome characterization and variability in some Iridaceae from Northeastern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Alves,Lânia Isis F.; Lima,Saulo Antônio A.; Felix,Leonardo P.
The chromosomes of 15 species of Iridaceae of the genera Alophia, Cipura, Eleutherine, Neomarica and Trimezia (subfamily Iridoideae) were examined after conventional Giemsa staining. The karyotypes of Alophia drummondii (2n = 14+1B, 28, 42 and 56), Cipura paludosa (2n = 14), C. xanthomelas (2n = 28) and Eleutherine bulbosa (2n = 12) were asymmetric; Neomarica candida, N. caerulea, N. humilis, N. glauca, N. gracilis, N. northiana and Neomarica sp. (2n = 18); N. cf. paradoxa (2n = 28), Trimezia fosteriana (2n = 52), T. martinicensis (2n = 54) and T. connata (2n = 82) were all generally symmetric. New diploid numbers of 2n = 56 for Alophia drummondii, 2n = 18 for N. candida, N. humilis, N. glauca, and N. gracilis, 2n = 28 for N. cf. paradoxa, and 2n = 82 for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iridaceae; Disploidy; Karyotypic evolution; Polyploidy; Asymmetrical karyotype.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200016

Is the American Zebu really Bos indicus?
Provedor de dados: Genetics and Molecular Biology
Autores: Meirelles,Flávio V.; Rosa,Artur J.M.; Lôbo,Raysildo B.; Garcia,Joaquim M.; Smith,Lawrence C.; Duarte,Francisco A.M.
The American continent was colonized in the 16th century by Europeans who first introduced cattle of Bos taurus origin. Accounts register introduction of Bos indicus cattle into South America in the 19th and continuing through the 20th century, and most reported imports were males derived from the Indian subcontinent. In the present study we show, by using mitochondrial DNA (mtDNA) polymorphism, major participation of matrilineages of taurus origin in the American Zebu purebred origin, i.e., 79, 73 and 100% for the Nellore, Gyr and Brahman breeds, respectively. Moreover, we have created a restriction map identifying polymorphism among B. taurus and B. indicus mtDNA using three restriction enzymes. Results are discussed concerning American Zebu origins and...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400013

Genetic basis of the resistance to Strongyloides venezuelensis (Nematoda, Rhabdiasidae) infection in mice (Mus musculus)
Provedor de dados: Genetics and Molecular Biology
Autores: Marra,Nelson Mendes; Amarante,Alessandro F.T.; Amarante,Mônica R.V.
We investigated the resistance to Strongyloides venezuelensis primary infection of mice strains NIH (resistant) and C57BL/6 (susceptible) and the F1 and F2 offspring of crosses between these strains. The mice were infected with 2000 larvae and seven days later were sacrificed for parasite recovery and counting. There was no statistically significant (p &gt; 0.05) sex effect on resistance. The F1 mice showed an intermediate mean number of parasites as compared to the parental NIH and C57BL6 strains. Out of 400 F2 mice, the 10% most resistant mice were infected with 21 to 97 parasites, while the 10% most susceptible mice were infected with 1027 to 1433 parasites. We also found that F2 mice with black fur (n = 72), the same color as the C57BL/6 susceptible...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mice; Strongyloides venezuelensis; Resistance; Genetics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100012

First record of Wolbachia in South American terrestrial isopods: prevalence and diversity in two species of Balloniscus (Crustacea, Oniscidea)
Provedor de dados: Genetics and Molecular Biology
Autores: Almerão,Mauricio Pereira; Fagundes,Nelson Jurandi Rosa; Araújo,Paula Beatriz de; Verne,Sébastien; Grandjean,Frédéric; Bouchon,Didier; Araújo,Aldo Mellender.
Wolbachia are endosymbiotic bacteria that commonly infect arthropods, inducing certain phenotypes in their hosts. So far, no endemic South American species of terrestrial isopods have been investigated for Wolbachia infection. In this work, populations from two species of Balloniscus (B. sellowii and B. glaber) were studied through a diagnostic PCR assay. Fifteen new Wolbachia 16S rDNA sequences were detected. Wolbachia found in both species were generally specific to one population, and five populations hosted two different Wolbachia 16S rDNA sequences. Prevalence was higher in B. glaber than in B. sellowii, but uninfected populations could be found in both species. Wolbachia strains from B. sellowii had a higher genetic variation than those isolated from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wolbachia; Prevalence; Diversity; South America; Oniscidea.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600013

Biometrical analyses of linolenic acid content of soybean seeds
Provedor de dados: Genetics and Molecular Biology
Autores: Gesteira,Abelmon da Silva; Schuster,Ivan; José,Inês Chamel; Piovesan,Newton Deniz; Viana,José Marcelo Soriano; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves.
The genetic reduction of linolenic acid levels increases the quality and stability of soybean oil. The objective of this study was to determine the inheritance and evaluate the nature and magnitude of gene effects on soybean seed linolenic acid level. Means and variances of F1, F2, and F3 generations were made from the cross between accession BARC-12 (low linolenic acid content) and the commercial Brazilian cultivar CAC-1 (normal linolenic acid content). The results demonstrated that linolenic acid content in soybean is under the genetic control of a small number of genes. The additive model explained the means for the three generations and for the parents. Non-allelic gene interactions had little effect on the determination of genotypic values for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean oil composition; Linolenic acid content; Biometrical analyses.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100011

Genotoxicity evaluation of domestic sewage in a municipal wastewater treatment plant
Provedor de dados: Genetics and Molecular Biology
Autores: Grisolia,Cesar Koppe; Oliveira,Adriano Barros Barata de; Bonfim,Helder; Klautau-Guimarães,Maria de Nazaré.
An evaluation of the genotoxicity of domestic sewage was carried out in a municipal wastewater treatment plant. The study was carried out using Allium cepa root tip cells through cytological parameters such as aberrant cells in anaphase-telophase and the mitotic index, and the fish species Oreochromis niloticus and Tilapia rendalli, through the index of micronuclei in peripheral erythrocytes. In the Allium test, each of the four stages of the wasterwater treatment routine was analyzed, i.e., crude sewage, primary effluent, secondary effluent, and tertiary effluent. O. niloticus and T. rendalli specimens were maintained in an aquarium continuously receiving the final effluent, in an open system, before being discharged into Lake Paranoá. For micronuclei...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Biomonitoring; Domestic sewage; Fish; Micronuclei; Mutagenicity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200026

A search for homologues of plant photoreceptor genes and their signaling partners in the sugarcane expressed sequence tag (Sucest) database
Provedor de dados: Genetics and Molecular Biology
Autores: Santelli,Roberto V.; Siviero,Fábio.
A search in the sugarcane expressed sequence tag (SUCEST) database for homologues of plant genes involved in photo-sensory mechanisms was carried out using the basic local alignment tool (BLAST). Our results shown that known elements (phytochromes, cryptochromes and phototoprin) present in Arabidopsis and other higher plants were detected with low e-values. We also searched for proteins interacting with photoreceptors in primary or downstream signaling events. One putative homologue for a protein postulated to be a primary element in phytochrome signaling pathways was identified, as were other candidates for downstream interacting factors.
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100008

Identification of environmental and genetic factors that influence warfarin time in therapeutic range
Provedor de dados: Genetics and Molecular Biology
Autores: Botton,Mariana R.; Viola,Patrícia P.; Meireles,Mariana R.; Bruxel,Estela M.; Zuchinali,Priccila; Bandinelli,Eliane; Rohde,Luis E.; Leiria,Tiago L. L.; Salamoni,Joyce Y. Y.; Garbin,Arthur P.; Hutz,Mara H.
Abstract Warfarin is an oral anticoagulant prescribed to prevent and treat thromboembolic disorders. It has a narrow therapeutic window and must have its effect controlled. Prothrombin test, expressed in INR value, is used for dose management. Time in therapeutic range (TTR) is an important outcome of quality control of anticoagulation therapy and is influenced by several factors. The aim of this study was to identify genetic, demographic, and clinical factors that can potentially influence TTR. In total,422 patients using warfarin were investigated. Glibenclamide co-medication and presence of CYP2C9*2 and/or *3 alleles were associated with higher TTR, while amiodarone, acetaminophen and verapamil co-medication were associated with lower TTR. Our data...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CYP2C9; VKORC1; ASPH; TTR; Warfarin.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000300302

Population structure of the Monocelis lineata (Proseriata, Monocelididae) species complex assessed by phylogenetic analysis of the mitochondrial Cytochrome c Oxidase subunit I (COI) gene
Provedor de dados: Genetics and Molecular Biology
Autores: Sanna,Daria; Lai,Tiziana; Francalacci,Paolo; Curini-Galletti,Marco; Casu,Marco.
Monocelis lineata consists of a complex of sibling species, widespread in the Mediterranean and Atlantic Ocean. Previous genetic analysis placed in evidence at least four sibling species. Nevertheless, this research was not conclusive enough to fully resolve the complex or to infer the phylogeny/phylogeography of the group. We designed specific primers aiming at obtaining partial sequences of the mtDNA gene Cytochrome c Oxidase subunit I (COI) of M. lineata, and have identified 25 different haplotypes in 32 analyzed individuals. The dendrogram generated by Neighbor-Joining analysis confirmed the differentiation between Atlantic and Mediterranean siblings, as well as the occurrence of at least two Mediterranean sibling species. Thus validated, the method...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microturbellaria; Sequencing; Sibling species; Phylogeography; Phylogeny.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400031

Polycythemia and Hb Coimbra [beta 99 (G1) Asp -&gt; Glu] in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Fattori,André; Kimura,Elza Miyuki; Albuquerque,Dulcinéia Martins de; Ogo,Satie Hatsushika; Stoppa,Graziela Renata; Martins,Juliana Touquinha; Lima,Carmen Silvia Passos; Saad,Sara Terezinha Ollala; Costa,Fernando Ferreira; Sonati,Maria de Fátima.
We report the clinical and laboratory findings concerning three unrelated Brazilian patients investigated for polycythemia, whose definitive diagnosis could only be established after the presence of Hb Coimbra (b99 Asp ® Glu) was demonstrated. This illustrates the importance of properly investigating hereditary hemoglobinopathies in cases of erythrocytosis because in some populations variants with high oxygen affinity may be more frequent than expected but go undetected when conventional electrophoresis is used as the sole detection procedure.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polycythemia; Erythrocytosis; Hb Coimbra; Beta-globin gene.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200002

Identifying sugarcane expressed sequences associated with nutrient transporters and peptide metal chelators
Provedor de dados: Genetics and Molecular Biology
Autores: Figueira,Antonio; Kido,Ederson Akio; Almeida,Raul Santin.
Plant nutrient uptake is an active process, requiring energy to accumulate essential elements at higher levels in plant tissues than in the soil solution, while the presence of toxic metals or excess of nutrients requires mechanisms to modulate the accumulation of ions. Genes encoding ion transporters isolated from plants and yeast were used to identify sugarcane putative homologues in the sugarcane expressed sequence tag (SUCEST) database. Five cluster consensi with sequence homology to plant high-affinity phosphate transporter genes were identified. One cluster consensus allowed the prediction of a full-length protein containing 541 amino acids, with 81% amino acid identity to the Nicotiana tabacum NtPT1 gene, consisting of 12 membrane-spanning domains...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100028

Asymmetry of frontal bristles and postocular setae in species and hybrids of the Anastrepha fraterculus complex (Diptera, Tephritidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,João Maria G.A.; Gouveia,Milson; Perondini,André L.P.; Selivon,Denise.
Asymmetry of the frontal bristles and postocular setae was studied in samples from natural populations and laboratory colonies of Anastrepha sp. 1 aff. fraterculus, of A. sp. 2 aff. fraterculus, and in F1 hybrids obtained from laboratory reciprocal crosses. Natural populations were sampled in a zone of sympatry and in two geographically distant regions with different climatic conditions. Asymmetry was scored as the differences between the number of bristles and of setae on the right and left sides of the head, males and females analyzed independently. The two traits exhibited variability according to the model of fluctuating asymmetry (FA). No significant differences among samples were found in the FA of frontal bristles. A significant FA was observed for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Developmental instability; Fluctuating asymmetry; Fruit flies; Insect.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100025

Rules extraction from neural networks applied to the prediction and recognition of prokaryotic promoters
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Scheila de Avila e; Gerhardt,Günther J.L.; Echeverrigaray,Sergio.
Promoters are DNA sequences located upstream of the gene region and play a central role in gene expression. Computational techniques show good accuracy in gene prediction but are less successful in predicting promoters, primarily because of the high number of false positives that reflect characteristics of the promoter sequences. Many machine learning methods have been used to address this issue. Neural Networks (NN) have been successfully used in this field because of their ability to recognize imprecise and incomplete patterns characteristic of promoter sequences. In this paper, NN was used to predict and recognize promoter sequences in two data sets: (i) one based on nucleotide sequence information and (ii) another based on stability sequence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neural network; Promoter; Rule extraction.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200031

Genotoxicity of titanium dioxide nanoparticles and triggering of defense mechanisms in Allium cepa
Provedor de dados: Genetics and Molecular Biology
Autores: Santos Filho,Ronaldo dos; Vicari,Taynah; Santos,Samuel A.; Felisbino,Karoline; Mattoso,Ney; Sant’Anna-Santos,Bruno Francisco; Cestari,Marta Margarete; Leme,Daniela Morais.
Abstract Titanium dioxide nanoparticles (TiO2NPs) are widely used and may impact the environment. Thus, this study used a high concentration of TiO2NP (1000 mg/L) to verify the defense mechanisms triggered by a plant system – an indicator of toxicity. Furthermore, this study aimed at completely characterizing TiO2NP suspensions to elucidate their toxic behavior. TiO2NPs were taken up by meristematic cells of Allium cepa, leading to slight inhibition of seed germination and root growth. However, severe cellular and DNA damages were observed in a concentration-dependent manner (10, 100, and 1000 mg/L). For this reason, we used the highest tested concentration (1000 mg/L) to verify if the plant cells developed defense mechanisms against the TiO2NPs and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal aberrations; Micronuclei; Nucleolar alterations; Cellular alterations; Nanoparticle phase-change.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300425

Role of error-prone DNA polymerases in spontaneous mutagenesis in Caulobacter crescentus
Provedor de dados: Genetics and Molecular Biology
Autores: Valencia,Alexy O.; Braz,Vânia S.; Magalhães,Magna; Galhardo,Rodrigo S.
Abstract Spontaneous mutations are important players in evolution. Nevertheless, there is a paucity of information about the mutagenic processes operating in most bacterial species. In this work, we implemented two forward mutational markers for studies in Caulobacter crescentus. We confirmed previous results in which A:T → G:C transitions are the most prevalent type of spontaneous base substitutions in this organism, although there is considerable deviation from this trend in one of the loci analyzed. We also investigated the role of dinB and imuC, encoding error-prone DNA polymerases, in spontaneous mutagenesis in this GC-rich organism. Both dinB and imuC mutant strains show comparable mutation rates to the parental strain. Nevertheless, both strains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DinB; ImuC; DnaE2; Spontaneous mutagenesis; Caulobacter crescentus.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100402

Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Fernanda Timm Seabra; Mello,Alexandre Silva de; Michelin,Kristiane; Coelho,Janice Carneiro.
The effect of four antibiotics (amikacin, clindamycin, cephalothin and vancomycin) was investigated considering that bacterial infection in fibroblasts cultures is a very frequent event. The investigation included the effect of the antibiotics on fibroblast growth and on the activity of the enzyme glucocerebrosidase. The antibiotics were added to the fibroblast cultures and cell growth was evaluated by counting the number of cells and their viability. After cell harvesting, the enzyme activity and content of protein were measured. The results allowed us to conclude that none of the antibiotics affected the cellular number nor the cellular viability. The content of protein decreased when cephalothin and clindamycin were added to the cultures, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amikacin; Cephalothin; Clindamycin; Vancomycin; Fibroblasts; Cell culture.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300023

Molecular evidence of polyphyletism in the plant genus Carum L. (Apiaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Papini,Alessio; Banci,Francesca; Nardi,Enio.
An analysis of internal transcribed spacer (ITS) DNA sequences of the four species of Carum L. (Apiaceae) known in Italy revealed that this genus is polyphyletic. Maximum parsimony with bootstrap resampling, maximum likelihood and Bayesian inference analyses resulted in three distinct clades: Carum carvi L. clustered within tribe Careae Baill. (former Aegopodium clade); Hellenocarum multiflorum (= Carum multiflorum), Carum heldreichii and Carum appuanum clustered within the tribe Pyramidoptereae Boiss.; and H. multiflorum and C. heldreichii formed a well supported clade. Since the sister group of H. multiflorum and C. heldreichii was Bunium elegans the autonomy of Hellenocarum from Carum is confirmed by our study. We also found that C. appuanum clustered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apiaceae; Carum; Hellenocarum; ITS; Phylogeny.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300029

Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum
Provedor de dados: Genetics and Molecular Biology
Autores: Podio,Maricel; Rodríguez,María P.; Felitti,Silvina; Stein,Juliana; Martínez,Eric J.; Siena,Lorena A.; Quarin,Camilo L.; Pessino,Silvina C.; Ortiz,Juan Pablo A.
In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen molecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence mapping...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apomixis; Chromosome walking; Gene mapping; Molecular markers.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500018

Microsatellite characterization of Cimarron Uruguayo dogs
Provedor de dados: Genetics and Molecular Biology
Autores: Gagliardi,Rosa; Silvia,Llambí; García,Cristina; Arruga,María Victoria.
Various genetic markers, including microsatellites, have been used to analyze the genetic polymorphism and heterozygosity in canine breeds. In this work, we used nine microsatellite markers to investigate the genetic variability in Cimarron Uruguayo dogs, the only officially recognized native canine breed in Uruguay. DNA from 30 Cimarron Uruguayo dogs from northeastern and southern Uruguay was analyzed. The allelic frequencies for each micro-satellite, the genetic variability and the consanguinity were calculated, as were the polymorphic information content (PIC) and the probability of exclusion (PE). All of the microsatellites studied were polymorphic. FH 2361, FH 2305 and PEZ 03 were the most informative, with PIC values &gt; 0.7, in agreement with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cimarron Uruguayo dog; Genetic characterization; Microsatellites.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100028

Evidence for biological inheritance of the eosinophil response to internal parasites in southeastern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Conti,Fátima; Dal' Colletto,Glória M. Duccini; Feitosa,Mary Furlan; Krieger,Henrique.
One hundred and seventy-seven individuals belonging to 120 complete or incomplete nuclear families from Bambui, in the State of Minas Gerais, southeastern Brazil, were studied in order to examine causes of variation in the eosinophil rate among subjects infested by intestinal worms with an extra-digestive cycle. Segregation analysis without correction for skewness showed that the hypothesis of the presence of an additive major gene was consistent with the data, although a dominant, recessive, or a multifactorial hypothesis could not account properly for the observed significant familial aggregation. The most parsimonious correction for skewness showed similar results, but could not distinguish between dominant and recessive models, although co-dominance...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400004

A genetic algorithm for the ligand-protein docking problem
Provedor de dados: Genetics and Molecular Biology
Autores: Magalhães,Camila S. de; Barbosa,Hélio J.C.; Dardenne,Laurent E.
We analyzed the performance of a real coded "steady-state" genetic algorithm (SSGA) using a grid-based methodology in docking five HIV-1 protease-ligand complexes having known three-dimensional structures. All ligands tested are highly flexible, having more than 10 conformational degrees of freedom. The SSGA was tested for the rigid and flexible ligand docking cases. The implemented genetic algorithm was able to dock successfully rigid and flexible ligand molecules, but with a decreasing performance when the number of ligand conformational degrees of freedom increased. The docked lowest-energy structures have root mean square deviation (RMSD) with respect to the corresponding experimental crystallographic structure ranging from 0.037 Å to 0.090 Å in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ligand-protein docking; Flexible docking; Genetic algorithms.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400022

A simple protocol for the extraction and sequence analysis of DNA from study skin of museum collections
Provedor de dados: Genetics and Molecular Biology
Autores: Moraes-Barros,Nadia de; Morgante,João Stenghel.
Museum collections have been widely used as sources of biological samples for molecular biology studies and there are several methodologies and techniques to obtain and analyze DNA from tissues archived in museums, but most of these protocols have been developed for a specific tissue or are commercial kits. We present a simple protocol for extracting and amplifying DNA segments from sloth museum specimens. With this simple protocol we analyzed DNA fragments from 64% of 64 skin samples from three-toed sloths (Bradypus variegatus and Bradypus tridactylus) archived in three different museums: 43 samples from the University of São Paulo Museum of Zoology (Museu de Zoologia da Universidade de São Paulo, MUZUSP) São Paulo, São Paulo, Brazil; 18 samples from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bradypus; DNA; Dried skins; Museum specimens.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600024

Estimation of genetic parameters for test-day milk yield in Holstein cows using a random regression model
Provedor de dados: Genetics and Molecular Biology
Autores: Cobuci,Jaime Araujo; Euclydes,Ricardo Frederico; Lopes,Paulo Sávio; Costa,Claudio Napolis; Torres,Robledo de Almeida; Pereira,Carmen Silva.
Test-day milk yield records of 11,023 first-parity Holstein cows were used to estimate genetic parameters for milk yield during different lactation periods. (Co)variance components were estimated using two random regression models, RRM1 and RRM2, and the restricted maximum likelihood method, compared by the likelihood ratio test. Additive genetic variances determined by RRM1 and additive genetic and permanent environmental variances estimated by RRM2 were described, using the Wilmink function. Residual variance was constant throughout lactation for the two models. The heritability estimates obtained by RRM1 (0.34 to 0.56) were higher than those obtained by RRM2 (0.15 to 0.31). Due to the high heritability estimates for milk yield throughout lactation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Random regression models; REML method; Genetic parameters; Test-day milk yield; Holstein cows.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100013

Genetic divergence in Tetragonisca angustula Latreille, 1811 (Hymenoptera, Meliponinae, Trigonini) based on rapd markers
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Rosana de Cássia; Nunes,Francis de Morais Franco; Campos,Ana Paula Soraggi; Vasconcelos,Soraya Matos de; Roubik,David; Goulart,Luiz Ricardo; Kerr,Warwick Estevam.
One of the commonest neotropical stingless bees is Tetragonisca angustula (Latreille, 1811), popularly known in Portuguese as jataí, which occurs in variable nesting sites from Mexico to Argentina. We used 18 primers to generate 218 RAPD markers which we used to determined the genetic distance between T. angustula populations from 25 localities in three different Latin America countries, using Tetragonisca weyrauchi from the Brazilian state of Acre and the common honey bee (Apis mellifera) as outgroups. Genetic distance, calculated as the Percentage of Dissimilarity (14%), based on all markers divided the T. angustula population into eastern (group 1) and western (group 2) groups. However, we were able to separate the two groups by using only two primers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tetragonisca angustula; Genetic divergence; Molecular markers; UPGMA.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200009

Population variability in some genes involving the haemostatic system: data on the general population of Corsica (France), Sardinia and Sicily (Italy)
Provedor de dados: Genetics and Molecular Biology
Autores: Falchi,Alessandra; Vacca,Lucia; Lopez Alomar,Antonio; Esteban,Esther; Memmi,Marc; Varesi,Laurent; Moral,Pedro; Vona,Giuseppe.
Three different population samples from Corsica (France), Sardinia and Sicily (Italy) were studied using nine genetic markers. For the first time, allele distributions of FGA TaqI, FGB Bcl I, FGB Hind III, PAI-1 Hind III, PLAT TPA-25, GPIIIa Taq I, GPIIb I/D 9bp, FVII HVR4 and FVII -323 10 bp markers, which are thought to be associated with cardiovascular disease risk, were studied in the general population of the three islands. The frequencies of the markers analysed in the present work show some peculiarities: the locus FVII HVR4 is characterized by the presence of a rare allele (H5), found in Corsicans and in Sardinians; the locus FBG BcII shows a low frequency of the B1 allele and the absence of the B1B1 genotype. The frequencies of some alleles have a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haemostatic genes; Genetic markers; Mediterranean populations.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200002

VDR polymorphisms influence immunological response in HIV-1+ individuals undergoing antiretroviral therapy
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Ronaldo Celerino da; Alves,Neyla Maria Pereira; Pereira,Jorge José de Souza; Coelho,Antonio Victor Campos; Arraes,Luiz Cláudio; Brandão,Lucas André Cavalcanti; Crovella,Sergio; Silva,Jaqueline de Azevêdo.
Abstract Vitamin D exerts an immuno-modulatory activity on several immune system cells through the vitamin D receptor (VDR). Herein, we verified that age and a therapeutic regimen containing protease inhibitors are associated with failures in antiretroviral therapies (ARVs). In addition, we assessed whether a VDR SNP (rs11568820: C allele and CC genotype) and GC (rs2228570-rs11568820) allelic combinations are associated with immunological failure (p &lt; 0.05). Our findings suggest a possible role of VDR SNPs on immunological failure in HIV-1+ individuals undergoing regular ARVs.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: VDR; HIV-1; ARVs; SNPs; CD4 recovery.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300351

On S.N. Bernstein's derivation of Mendel's Law and 'rediscovery' of the Hardy-Weinberg distribution
Provedor de dados: Genetics and Molecular Biology
Autores: Stark,Alan; Seneta,Eugene.
Around 1923 the soon-to-be famous Soviet mathematician and probabilist Sergei N. Bernstein started to construct an axiomatic foundation of a theory of heredity. He began from the premise of stationarity (constancy of type proportions) from the first generation of offspring. This led him to derive the Mendelian coefficients of heredity. It appears that he had no direct influence on the subsequent development of population genetics. A basic assumption of Bernstein was that parents coupled randomly to produce offspring. This paper shows that a simple model of non-random mating, which nevertheless embodies a feature of the Hardy-Weinberg Law, can produce Mendelian coefficients of heredity while maintaining the population distribution. How W. Johannsen's...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bernstein; Mendel; Hardy-Weinberg; Axiomatic foundations; Coefficients of heredity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300002

Growth trends, genotype-environment interaction and genetic gains in six-year-old rubber tree clones (Hevea) in São Paulo State, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Paulo de Souza; Bataglia,Ondino Cleante; Santos,Wagner Rodrigues dos; Ortolani,Altino Aldo; Segnini Jr.,Ivo; Shikasho,Edson Hideaki.
Budwood from seven different clones of rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Müell. Arg.] was planted in replicated trials in four different test sites in the plateau region of the state of São Paulo, Brazil. The clones represented a range of imported germplasm, whereas the locations were selected to represent a range of rubber growing areas. Girths were measured for six years consecutively, before the initiation of tapping for latex. Total number of latex vessel rings (lvr) and bark thickness (bt) were measured at six years. The largest average for all characters was observed in Matão. Overall means for girth, total number of latex vessel rings and bark thickness at six years were 35.26 cm, 11.30 units and 4.83 mm, respectively. Both...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100019

Cytogenetic studies of three Lycosidae species   from Argentina (Arachnida, Araneae)
Provedor de dados: Genetics and Molecular Biology
Autores: Chemisquy,María A.; Gil,Sergio G. Rodríguez; Scioscia,Cristina L.; Mola,Liliana M.
Cytogenetic studies of the family Lycosidae (Arachnida: Araneae) are scarce. Less than 4% of the described species have been analyzed and the male haploid chromosome numbers ranged from 8+X 1  X  2  to 13+X 1  X  2 . Species formerly classified as  Lycosa  were the most studied ones. Our aim in this work was to perform a comparative analysis of the meiosis in " Lycosa "  erythrognatha  Lucas, " Lycosa "  pampeana  Holmberg and  Schizocosa malitiosa  (Tullgren). We also compared male and female karyotypes and characterized the heterochromatin of " L. "  erythrognatha . The males of the three species had 2n = 22, n = 10+X 1 X 2 , all the chromosomes were telocentric and there was generally a single chiasma per bivalent. In " Lycosa "  pampeana , which is...
Palavras-chave: Meiosis; C-banding; Fluorochrome staining; Karyotype; Spiders; "Lycosa"  and Schizocosa.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500010

Using genetic diversity information to establish core collections of Stylosanthes capitata and Stylosanthes macrocephala
Provedor de dados: Genetics and Molecular Biology
Autores: Santos-Garcia,Melissa Oliveira; Toledo-Silva,Guilherme de; Sassaki,Rodrigo Possidonio; Ferreira,Thais Helena; Resende,Rosângela Maria Simeão; Chiari,Lucimara; Karia,Cláudio Takao; Carvalho,Marcelo Ayres; Faleiro,Fábio Gelape; Zucchi,Maria Imaculada; Souza,Anete Pereira de.
Stylosanthes species are important forage legumes in tropical and subtropical areas. S. macrocephala and S. capitata germplasm collections that consist of 134 and 192 accessions, respectively, are maintained at the Brazilian Agricultural Research Corporation Cerrados (Embrapa-Cerrados). Polymorphic microsatellite markers were used to assess genetic diversity and population structure with the aim to assemble a core collection. The mean values of H O and H E for S. macrocephala were 0.08 and 0.36, respectively, whereas the means for S. capitata were 0.48 and 0.50, respectively. Roger's genetic distance varied from 0 to 0.83 for S. macrocephala and from 0 to 0.85 for S. capitata. Analysis with STRUCTURE software distinguished five groups among the S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stylosanthes; Tropical forage; Microsatellites; Genetic diversity; Core collection.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500020

DNA methylation dynamics in the rat EGF gene promoter after partial hepatectomy
Provedor de dados: Genetics and Molecular Biology
Autores: Li,Deming; Fan,Jinyu; Li,Ziwei; Xu,Cunshuan.
Epidermal growth factor (EGF), a multifunctional growth factor, is a regulator in a wide variety of physiological processes. EGF plays an important role in the regulation of liver regeneration. This study was aimed at investigating the methylation level of EGF gene throughout liver regeneration. DNA of liver tissue from control rats and partial hepatectomy (PH) rats at 10 time points was extracted and a 354 bp fragment including 10 CpG sites from the transcription start was amplified after DNA was modified by sodium bisulfate. The result of sequencing suggested that methylation ratio of four CpG sites was found to be significantly changed when PH group was compared to control group, in particular two of them were extremely striking. mRNA expression of EGF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylation dynamics; Epidermal growth factor; Liver regeneration.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300017

The challenges of organizing an international course in Latin America
Provedor de dados: Genetics and Molecular Biology
Autores: Vairo,Filippo; López,Mónica Luján; Cruz,Carolina Uribe; Corrêa,Priscila Gomes; Baldo,Guilherme.
The Latin American School of Human and Medical Genetics (ELAG) is the main course of its kind in the genetics field in Latin America. Here we describe the main challenges regarding the organization of such event, including how we obtain funding and how we proceed with student selection. Thus, we aim to share our experience with other groups that intend to follow this format to create similar events in other areas in this region of the world.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetics in Latin America; Funding; Educational initiatives.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200002

Chromosome complement and meiosis in three species of the Neotropical bug genus Antiteuchus (Heteroptera, Pentatomidae, Discocephalinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Lanzone,Cecilia; Souza,Maria José de.
Orcein staining of spermatocytes was used to study the meiotic behavior of holocentric chromosomes in three member of the genus Antiteuchus (commonly known as stink bugs). We describe and illustrate the karyotype of Antiteuchus mixtus, A. sepulcralis and A. macraspis which were cytogenetically characterized as having a diploid number of 2n = 14 and an XY sex chromosome system showing pre-reductional meiosis for autosomes and post-reductional meiosis for sex chromosomes. These species were also shown to have a long diffuse stage during meiotic prophase I and aberrant harlequin-type meiocytes. The chiasma frequency was also analyzed for two of the three species studied.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spermatogenesis; Holokinetic chromosomes; Sex chromosomes; Meiosis; Diffuse stage.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100011

Genotype-phenotype correlation in cystic fibrosis patients bearing [H939R;H949L] allele
Provedor de dados: Genetics and Molecular Biology
Autores: Polizzi,Angela; Tesse,Riccardina; Santostasi,Teresa; Diana,Anna; Manca,Antonio; Logrillo,Vito Paolo; Cazzato,Maria Domenica; Pantaleo,Maria Giuseppa; Armenio,Lucio.
Cystic fibrosis (CF) is caused by CFTR (cystic fibrosis transmembrane conductance regulator) gene mutations. We ascertained five patients with a novel complex CFTR allele, with two mutations, H939R and H949L, inherited in cis in the same exon of CFTR gene, and one different mutation per patient inherited in trans in a wide population of 289 Caucasian CF subjects from South Italy. The genotype-phenotype relationship in patients bearing this complex allele was investigated. The two associated mutations were related to classical severe CF phenotypes.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CFTR; Complex allele; Cystic fibrosis; Phenotype.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300008

Genetic characterization of native and introduced populations of the neotropical cichlid genus Cichla in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Daniel Cardoso de; Oliveira,Denise Aparecida Andrade de; Santos,José Enemir dos; Teske,Peter; Beheregaray,Luciano B.; Schneider,Horacio; Sampaio,Iracilda.
A molecular phylogenetic analysis based on mitochondrial 16S ribosomal DNA and Control Region sequences from native and introduced populations was undertaken, in order to characterize the introduction of Cichla (peacock bass or tucunaré) species in Brazil. Mitochondrial DNA haplotypes found in introduced fish from Minas Gerais state (southeastern Brazil) clustered only with those from native species of the Tocantins River (Cichla piquiti and C. kelberi), thereby suggesting a single or, at most, few translocation acts in this area, even though with fish from the same source-population. Our study contributes to an understanding of the introduction of Cichla in regions of Brazil outside the Amazon basin, and adds phylogenetic data to the recently describe...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peacock bass or tucunaré; MtDNA; Invasive populations; Amazon basin; Minas Gerais.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300025

Programa GENES: Aplicativo Computacional em Estatística Aplicada à Genética (GENES - Software for Experimental Statistics in Genetics)
Provedor de dados: Genetics and Molecular Biology
Autores: Cruz,Cosme Damião.
The main purpose of the GENES software is to help people working with genetic analysis and data processing in breeding programs, using several biometrics models. This software has several help windows that are very friendly to the user. More information about this program is available in the book" Programa GENES - Aplicativo Computacional em Genética e Estatística, 442. 1997". Purchase orders are welcome at the following address: editora@mail.ufv.br. Shareware copies of the GENES software are available at http://www.genetica.dbg.ufv.br.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100022

Paternity testing and behavioral ecology: a case study of jaguars (Panthera onca) in Emas National Park, Central Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Soares,Thannya Nascimento; Telles,Mariana P.C.; Resende,Lucileide V.; Silveira,Leandro; Jácomo,Anah Tereza A.; Morato,Ronaldo G.; Diniz-Filho,José Alexandre F.; Eizirik,Eduardo; Brondani,Rosana P.V.; Brondani,Claudio.
We used microsatellite loci to test the paternity of two male jaguars involved in an infanticide event recorded during a long-term monitoring program of this species. Seven microsatellite primers originally developed for domestic cats and previously selected for Panthera onca were used. In order to deal with uncertainty in the mother's genotypes for some of the loci, 10000 values of W were derived by simulation procedures. The male that killed the two cubs was assigned as the true sire. Although the reasons for this behavior remain obscure, it shows, in principle, a low recognition of paternity and kinship in the species. Since the two cubs were not very young, one possibility is that the adult male did not recognize the cubs and killed them for simple...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecological stress; Infanticide; Jaguar; Microsatellites; Paternity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400025

Evaluation of occupational genotoxic risk in a Brazilian hospital
Provedor de dados: Genetics and Molecular Biology
Autores: Maluf,Sharbel Weidner; Erdtmann,Bernardo.
Many therapeutic, diagnostic and prophylactic procedures used in hospitals are of potential genetic risk. An evaluation was made of genotoxic occupational risk in 42 workers from the Hospital de Clínicas de Porto Alegre, RS, Brazil, who had been occupationally exposed to lead (solder), ethylene oxide (sterilization area), antineoplastic drugs (nurses and pharmacists) or ionizing radiation. They were compared with 42 unexposed individuals. There was an increase in the frequency of binucleated cytochalasin-blocked lymphocytes with micronuclei, though it was not significant (P = 0.058). The groups exposed to antineoplastic drugs and radiation had a significant increase in micronuclei frequency (P = 0.038 and P = 0.022, respectively). The high frequencies of...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200040

Genetic variability and population structure in loci related to milk production traits in native Argentine Creole and commercial Argentine Holstein cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Golijow,C.D.; Giovambattista,G.; Rípoli,M.V.; Dulout,F.N.; Lojo,M.M.
Many cattle breeds have been subjected to high selection pressure for production traits. Consequently, population genetic structure and allelic distribution could differ in breeds under high selection pressure compared to unselected breeds. Analysis of &lt;FONT FACE="Symbol"&gt;k&lt;/font&gt;-casein, &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;S1-casein and prolactin gene frequencies was made for Argentine Creole (AC) and Argentine Holstein (AH) cattle herds. The calculated FST values measured the degree of genetic differentiation of subpopulations, depending on the variances of gene frequencies.The AC breed had considerably more variation among herds at the &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;S1-casein and &lt;FONT FACE="Symbol"&gt;k&lt;/font&gt;-casein...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300018

Genetic characterization and phylogeography of the wild boar Sus scrofa introduced into Uruguay
Provedor de dados: Genetics and Molecular Biology
Autores: García,Graciela; Vergara,Julia; Lombardi,Raúl.
The European wild boar Sus scrofa was first introduced into Uruguay, in southern South America during the early decades of the last century. Subsequently, and starting from founder populations, its range spread throughout the country and into the neighbouring Brazilian state Rio Grande do Sul. Due to the subsequent negative impact, it was officially declared a national pest. The main aim in the present study was to provide a more comprehensive scenario of wild boar differentiation in Uruguay, by using mtDNA markers to access the genetic characterization of populations at present undergoing rapid expansion. A high level of haplotype diversity, intermediate levels of nucleotide diversity and considerable population differentiation, were detected among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uruguayan wild boar.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200027

A recombination point is conserved in the mitochondrial genome of higher plant species and located downstream from the cox2 pseudogene in Solanum tuberosum L.
Provedor de dados: Genetics and Molecular Biology
Autores: Tada,Susely F.S.; Souza,Anete P.
The potato (Solanum tuberosum L.) mitochondrial cox3/sdh4/pseudo-cox2 gene cluster has previously been identified by heterologous hybridization using a Marchantia polymorpha sdh4 probe. In our present study we used Southern blotting using sdh4 and cox2 probes to show that the sdh4 and cox2 genes are clustered in the mitochondria of potato, soybean and pea. Northern blotting revealed cotranscription of sdh4 and cox2 in potato but not in cauliflower, indicating that these genes are not clustered in cauliflower. A putative recombination point was detected downstream of the cox2 pseudogene (pseudo-cox2) in potato mitochondrial DNA (mtDNA). This sequence corresponds to a 32 bp sequence which appears to be well-conserved and is adjacent to the terminals of some...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial DNA; Rearrangement; Plants; Solanum tuberosum; Cox2.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100017

The lowest diploid number in Testudines: Banding patterns, telomeric and 45S rDNA FISH in Peltocephalus dumerilianus, 2n = 26 and FN = 52 (Pleurodira, Podocnemididae)
Provedor de dados: Genetics and Molecular Biology
Autores: Ventura,Karen; Moreira,Camila N; Moretti,Renata; Yonenag-Yassuda,Yatiyo; Rodrigues,Miguel T.
The karyotype of the big-headed Amazon River turtle, Peltocephalus dumerilianus, is characterized based on a sample of seven juveniles from Reserva Biológica do Rio Trombetas, Pará State, Brazil (1°30' S, 56°34' W). Here we present the first results on GTG and CBG-banding patterns, Ag-NOR staining and FISH, with telomeric and 45S rDNA sequences as probes. A cytogenetic comparison with related Podocnemidae is also provided.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Big-headed Amazon River turtle; Molecular cytogenetics; Pelomedusoides.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100011

Cytogenetic comparison of Podocnemis expansa and Podocnemis unifilis: a case of inversion and duplication involving constitutive heterochromatin
Provedor de dados: Genetics and Molecular Biology
Autores: Gunski,Ricardo José; Cunha,Isabel Souza; Degrandi,Tiago Marafiga; Ledesma,Mario; Garnero,Analía Del Valle.
Podocnemis expansa and P. unifilis present 2n = 28 chromosomes, a diploid number similar to those observed in other species of the genus. The aim of this study was to characterize these two species using conventional staining and differential CBG-, GTG and Ag-NOR banding. We analyzed specimens of P. expansa and P. unifilis from the state of Tocantins (Brazil), in which we found a 2n = 28 and karyotypes differing in the morphology of the 13th pair, which was submetacentric in P. expansa and telocentric in P. unifilis. The CBG-banding patterns revealed a heterochromatic block in the short arm of pair 13 of P. expansa and an interstitial one in pair 13 of P. unifilis, suggesting a pericentric inversion. Pair 14 of P. unifilis showed an insterstitial band in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome banding; Ag-NORs; Karyotype; Chelonids.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300009

Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum
Provedor de dados: Genetics and Molecular Biology
Autores: Cervelatti,Eliane P.; Ferreira-Nozawa,Monica S.; Aquino-Ferreira,Roseli; Fachin,Ana Lúcia; Martinez-Rossi,Nilce M.
The electrophoretic karyotype of the dermatophyte Trichophyton rubrum was established using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Five chromosomal bands of approximately 3.0 to 5.8 megabase pairs (Mbp) each were observed and together indicated that 22.05 Mbp of the total genome are organized as chromosomal macromolecules. In addition to establishing the number and size of T. rubrum chromosomes, these results open perspectives for the construction of chromosome-specific libraries and for the physical mapping of genes of interest, thus permitting future gene linkage studies in this pathogen. A detailed understanding of the karyotype and genomic organization of T. rubrum should contribute to further genetic, taxonomic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichophyton rubrum; Fungal chromosomes; Electrophoretic karyotype; Dermatophyte; Contour-clamped homogeneous electric field.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100016

A systematic scoping review of the genetic ancestry of the Brazilian population
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Aracele Maria de; Resende,Sarah Stela; Sousa,Taís Nóbrega de; Brito,Cristiana Ferreira Alves de.
Abstract The genetic background of the Brazilian population is mainly characterized by three parental populations: European, African, and Native American. The aim of this study was to overview the genetic ancestry estimates for different Brazilian geographic regions and analyze factors involved in these estimates. In this systematic scoping review were included 51 studies, comprehending 81 populations of 19 states from five regions of Brazil. To reduce the potential of bias from studies with different sampling methods, we calculated the mean genetic ancestry weighted by the number of individuals. The weighted mean proportions of European, African, and Native American ancestries were 68.1%, 19.6%, and 11.6%, respectively. At the regional level, the highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scoping review; Genetic ancestry; Brazilian population; Genetic admixture.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400495

Nonparametric stability methods for interpreting genotype by environment interaction of bread wheat genotypes (Triticum aestivum L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Akcura,Mevlut; Kaya,Yuksel.
Evaluation of performance stability and high yield is essential for yield trials conducted in different environments. We determined the stability of 10 bread wheat (Triticum aestivum L.) genotypes (5 cultivars and 5 advanced lines) using nonparametric stability measures and compared nonparametric stability statistics across 19 environments in the Central Anatolian Region of Turkey. Experiments were setup in a randomized complete block design with four replications. Nonparametric stability measures revealed that AL-2, Karahan-99 and Altay-2000 were the most stable and well adapted genotypes across the 19 environments. In addition, it was concluded that graphs of mean grain yield (t ha-1) vs. nonparametric measures (Si(¹), Si(²), S(³), Si(6) and σgy) values...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bread wheat (Triticum aestivum L.); Nonparametric stability analysis; Yield.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500018

Variation and genetic structure of Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations based on ISSR pattern
Provedor de dados: Genetics and Molecular Biology
Autores: Nascimento,Marcília A.; Batalha-Filho,Henrique; Waldschmidt,Ana M.; Tavares,Mara G.; Campos,Lucio A.O.; Salomão,Tânia M.F.
For a study of diversity and genetic structuring in Melipona quadrifasciata, 61 colonies were collected in eight locations in the state of Minas Gerais, Brazil. By means of PCR analysis, 119 ISSR bands were obtained, 80 (68%) being polymorphic. He and H B were 0.20 and 0.16, respectively. Two large groups were obtained by the UPGMA method, one formed by individuals from Januária, Urucuia, Rio Vermelho and Caeté and the other by individuals from São João Del Rei, Barbacena, Ressaquinha and Cristiano Otoni. The Φst and θB values were 0.65 and 0.58, respectively, thereby indicating high population structuring. UPGMA grouping did not reveal genetic structuring of M. quadrifasciata in function of the tergite stripe pattern. The significant correlation between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hymenoptera; Melipona; Genetic differentiation; ISSR markers; Population structure.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200031

Molecular analyses reveal the occurrence of three new sympatric lineages of velvet worms (Onychophora: Peripatidae) in the eastern Amazon basin
Provedor de dados: Genetics and Molecular Biology
Autores: Cunha,Williana T. R.; Santos,Rita C. O.; Araripe,Juliana; Sampaio,Iracilda; Schneider,Horacio; Rêgo,Péricles S.
Abstract In the present study, we investigated the possible existence of new lineages of peripatids through comparisons between known Neotropical species and specimens obtained from two locations in Pará, a state in eastern Brazilian Amazonia using a molecular approach based on sequences of the mtDNA genes COI, 16Sr RNA, and 18S RNA. The analyses included also sequences of Asian and African taxa for a more systematic understanding of the phylogenetic relationships within the group. The analysis of the COI, 16S rRNA and 18S RNA sequences permitted the identification of three distinct lineages (A, B and C) based on two different phylogenetic approaches (Bayesian methods and ML). The three lineages presented here are completely distinct from all other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazonia; New lineages; Peripatidae; Phylogeny; Onychophora.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100147

Micronuclei induced by reverse transcriptase inhibitors in mononucleated and binucleated cells as assessed by the cytokinesis-block micronucleus assay
Provedor de dados: Genetics and Molecular Biology
Autores: Lourenço,Eloir D.; Amaral,Viviane S. do; Lehmann,Mauricio; Dihl,Rafael R.; Schmitt,Virginia M.; Cunha,Kênya S.; Reguly,Maria L.; Andrade,Heloisa H.R. de.
This study evaluated the clastogenic and/or aneugenic potential of three nucleoside reverse transcriptase inhibitors (zidovudine - AZT, lamivudine - 3TC and stavudine - d4T) using the cytokinesis-block micronucleus (CBMN) assay in human lymphocyte cultures. All three inhibitors produced a positive response when tested in binucleated cells. The genotoxicity of AZT and 3TC was restricted to binucleated cells since there was no significant increase in the frequency of micronuclei in mononucleated cells. This finding indicated that AZT and 3TC caused chromosomal breakage and that their genotoxicity was related to a clastogenic action. In addition to the positive response observed with d4T in binucleated cells, this drug also increased the frequency of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lamivudine; Micronucleus assay; Stavudine; Transcriptase inhibitors; Zidovudine.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400024

The new world of RNAs
Provedor de dados: Genetics and Molecular Biology
Autores: Dogini,Danyella Barbosa; Pascoal,Vinícius D'Avila Bittencourt; Avansini,Simoni Helena; Vieira,André Schwambach; Pereira,Tiago Campos; Lopes-Cendes,Iscia.
One of the major developments that resulted from the human genome sequencing projects was a better understanding of the role of non-coding RNAs (ncRNAs). NcRNAs are divided into several different categories according to size and function; however, one shared feature is that they are not translated into proteins. In this review, we will discuss relevant aspects of ncRNAs, focusing on two main types: i) microRNAs, which negatively regulate gene expression either by translational repression or target mRNA degradation, and ii) small interfering RNAs (siRNAs), which are involved in the biological process of RNA interference (RNAi). Our knowledge regarding these two types of ncRNAs has increased dramatically over the past decade, and they have a great potential...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-coding RNAs; MicroRNAs; RNA interferance; RNA-based drugs.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200014

DNA damage in the kidney tissue cells of the fish Rhamdia quelen after trophic contamination with aluminum sulfate
Provedor de dados: Genetics and Molecular Biology
Autores: Klingelfus,Tatiane; Costa,Paula Moiana da; Scherer,Marcos; Cestari,Marta Margarete.
Abstract Even though aluminum is the third most common element present in the earth's crust, information regarding its toxicity remains scarce. It is known that in certain cases, aluminum is neurotoxic, but its effect in other tissues is unknown. The aim of this work was to analyze the genotoxic potential of aluminum sulfate in kidney tissue of the fish Rhamdia quelen after trophic contamination for 60 days. Sixty four fish were subdivided into the following groups: negative control, 5 mg, 50 mg and 500 mg of aluminum sulfate per kg of fish. Samples of the posterior kidney were taken and prepared to obtain mitotic metaphase, as well as the comet assay. The three types of chromosomal abnormalities (CA) found were categorized as chromatid breaks,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Metal contamination; Chromosomal abnormalities; Comet assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400499

Genotoxicity and antigenotoxicity assessment of shiitake (Lentinula edodes (Berkeley) Pegler) using the Comet assay
Provedor de dados: Genetics and Molecular Biology
Autores: Miyaji,CK; Jordão,BQ; Ribeiro,LR; Eira,AF; Cólus,IMS.
The mushroom shiitake (Lentinula edodes (Berkeley) Pegler) is been widely consumed in many countries, including Brazil, because of its pleasant flavor and reports of its therapeutic properties, although there is little available information on the genotoxicity and/or antigenotoxicity of this mushroom. We used the Comet assay and HEp-2 cells to evaluate the in vitro genotoxic and antigenotoxic activity of aqueous extracts of shiitake prepared in three different concentrations (0.5, 1.0 and 1.5 mg/mL) and three different temperatures (4, 22 and 60 °C), using methyl methanesulfonate (MMS) as a positive control and untreated cells as a negative control. Two concentrations (1.0 and 1.5 mg/mL) of extract prepared at 4 °C and all of the concentrations prepared at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shiitake; Comet assay; HEp-2 cells; Genotoxicity; Antigenotoxicity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100018

A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants
Provedor de dados: Genetics and Molecular Biology
Autores: Bernd-Souza,R.B.; Sa,M.F. Grossi de; Ellis,D.D.; McCown,B.H.
The effects of an animal RNase fused to the late cotton pollen-specific promoter G9 in a plant system were investigated. Expression of the chimeric genes G9-uidA and G9-RNase in tobacco plants showed that the 1.2-kb promoter fragment of the G9 gene was sufficient to maintain tissue and temporal specificity in a heterologous system. GUS (beta-glucuronidase) expression was detected only in pollen from anther stage 6 through anthesis, with maximal GUS activity in pollen from stage 10 anthers. Investigating the effects of the rat RNase on pollen viability at stage 10, we found that pollen viability was reduced from 79 to 8% and from 89 to 40%, in pollen germination and fluoresceine diacetate assays, respectively, in one G9-RNase transgenic line, suggesting a...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200032

Meiotic behavior of Adesmia DC.  (Leguminosae-Faboideae) species native  to Rio Grande do Sul, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Coelho,Liliana Gressler May; Battistin,Alice.
Meiotic behavior in Adesmia DC. is described for the first time. The study encompassed twelve populations of seven Adesmia DC. species native to Rio Grande do Sul, Brazil. Populations with 2n = 2x = 20 are A. securigerifolia 9615, A. riograndensis 9590 (subnudae), A. latifolia 1568, 1775, 15025, A. bicolor JB-UFSM, A. incana var. incana 9636, 10288, A. punctata var. hilariana 6885, 10812, and A. tristis 10757. A. incana var. incana 9637 is a tetraploid with 2n = 4x = 40. The material was stained with 1% acetic orcein. The meiotic behavior of the populations studied was considered normal. The meiotic index (MI) and the estimates of pollen grain viability were above 95%, except for A. latifolia 1568 (MI = 89%). The present data indicate that these plants are...
Tipo: Info:eu-repo/semantics/other
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300020

Genotoxicity and mutagenicity of Echinodorus macrophyllus (chapéu-de-couro) extracts
Provedor de dados: Genetics and Molecular Biology
Autores: Vidal,Leonardo S.; Alves,Adriana M.; Kuster,Ricardo M.; Lage,Claudia; Leitão,Alvaro C.
Echinodorus macrophyllus, commonly known as chapéu-de-couro, is a medicinal plant used in folk medicine to treat inflammation and rheumatic diseases. In this work, we used short-term bacterial assays based on the induction of SOS functions to examine the genotoxicity and mutagenicity of an aqueous extract of E. macrophyllus leaves. Whole extract and an ethyl acetate fraction showed similar genotoxicity and caused an ~70-fold increase in lysogenic induction. The extract also gave a positive result in the SOS chromotest with an increase of 12-fold in β-Galactosidase enzymatic units. There was a strong trend towards base substitutions and frameshifts at purine sites in the mutations induced by the extract in Escherichia coli (CC103 and CC104 strains) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Echinodorus macrophyllus; Genotoxicity; Mutagenesis; Reactive oxygen species.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300028

A SNP in 5′ untranslated region of CD40 gene is associated with an increased risk of ischemic stroke in a Chinese population: a case-control study
Provedor de dados: Genetics and Molecular Biology
Autores: Huang,Hua-Tuo; Guo,Jing; Xiang,Yang; Chen,Jian-Ming; Luo,Hong-Cheng; Meng,Lan-Qing; Wei,Ye-Sheng.
Abstract Cluster of differentiation 40 (CD40), the receptor for CD154, is a member of the tumor necrosis factor (TNF) receptor superfamily. Several studies have been conducted to investigate the effect of the CD40 rs1883832 polymorphism on atherosclerotic disease in different population; however, inconsistent results were obtained. In this study, we investigated the association of four polymorphisms (rs1883832, rs13040307, rs752118 and rs3765459) of CD40 gene and their effect on CD40 expression with the risk of ischemic stroke (IS) in a Chinese population. Three hundred and eighty patients with IS and 450 control subjects were included in the study. The CD40 polymorphisms were discriminated by Snapshot SNP genotyping assay. Serum soluble CD40 (sCD40)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CD40; Gene; Polymorphism; Ischemic stroke.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300442

Analysis of expressed sequence tags from Citrus sinensis L. Osbeck infected with Xylella fastidiosa
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Targon,Maria Luisa P.N.; Carlos,Eduardo F.; Locali-Fabris,Eliane C.; Amaral,Alexandre M.; Freitas-Astúa,Juliana; Silva-Pinhati,Ana Carla O.; Boscariol-Camargo,Raquel L.; Berger,Irving J.; Rodrigues,Carolina M.; Reis,Marcelo S.; Machado,Marcos A.
In order to understand the genetic responses resulting from physiological changes that occur in plants displaying citrus variegated chlorosis (CVC) symptoms, we adopted a strategy of comparing two EST libraries from sweet orange [Citrus sinensis (L.) Osbeck]. One of them was prepared with plants showing typical CVC symptoms caused by Xylella fastidiosa and the other with non-inoculated plants. We obtained 15,944 ESTs by sequencing the two cDNA libraries. Using an in silico hybridization strategy, 37 genes were found to have significant variation at the transcriptional level. Within this subset, 21 were up-regulated and 16 were down-regulated in plants with CVC. The main functional categories of the down-regulated transcripts in plants with CVC were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sweet orange; EST; CVC; Compatible interaction.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500023

Association of polymorphisms in the heparanase gene (HPSE) with hepatocellular carcinoma in Chinese populations
Provedor de dados: Genetics and Molecular Biology
Autores: Yu,Lixia; Zhang,Xiaoai; Zhai,Yun; Zhang,Hongxing; Yue,Wei; Zhang,Xiumei; Wang,Zhifu; Zhou,Hong; Zhou,Gangqiao; Gong,Feng.
Abstract Heparanase activity is involved in cancer growth and development in humans and single nucleotide polymorphisms (SNPs) in the heparanase gene (HPSE) have been shown to be associated with tumors. In this study, we investigated whether SNPs in HPSE were a risk factor for hepatocellular carcinoma (HCC) by undertaking a comprehensive haplotype-tagging, case-control study. For this, six haplotype-tagging SNPs (htSNPs) in HPSE were genotyped in 400 HCC patients and 480 controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. A log-additive model revealed significant correlations between the HPSE polymorphisms rs12331678 and rs12503843 and the risk of HCC in the overall samples (p = 0.0046 and p = 0.0055). When...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic association; Heparanase; Hepatocellular carcinoma; HPSE gene; PCR-RFLP.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500743

Aggregation tendency in small groups of Drosophila melanogaster
Provedor de dados: Genetics and Molecular Biology
Autores: del Solar Osses,Eduardo.
A study was made of the spatial pattern of the progeny produced in boxes with 25 oviposition tubes, by groups of six Drosophila melanogaster females during 24, 72 and 216 h. Each female was fertilized by a male carrier of a dominant mutation in such a manner that the behavior of each female of the group as well as that of each group could be determined by a study of their progeny. The pattern of spatial distribution of the progeny was aggregated, reaching its highest value at 72 h. Gregarious behavior of individuals was defined by the number of sites among which they disseminated their progeny in a 24 h-cycle; while for groups it was estimated by the variance/mean ratio. The fertility, egg-adult development time and mortality rate were recorded....
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100006

Genes involved in cell division in mycoplasmas
Provedor de dados: Genetics and Molecular Biology
Autores: Alarcón,Frank; Vasconcelos,Ana Tereza Ribeiro de; Yim,Lucia; Zaha,Arnaldo.
Bacterial cell division has been studied mainly in model systems such as Escherichia coli and Bacillus subtilis, where it is described as a complex process with the participation of a group of proteins which assemble into a multiprotein complex called the septal ring. Mycoplasmas are cell wall-less bacteria presenting a reduced genome. Thus, it was important to compare their genomes to analyze putative genes involved in cell division processes. The division and cell wall (dcw) cluster, which in E. coli and B. subtilis is composed of 16 and 17 genes, respectively, is represented by only three to four genes in mycoplasmas. Even the most conserved protein, FtsZ, is not present in all mycoplasma genomes analyzed so far. A model for the FtsZ protein from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell division; Mycoplasma spp; Genomes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200003

New brazilian member of the USA National Academy of Sciences
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/report
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200007

Comparative karyotype analysis in diploid and triploid Dolichoplana carvalhoi (Tricladida, Terricola, Rhynchodemidae) from Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Alvarez,Luciana; Almeida,Eunice Judith Cardoso de.
In this work, we present cytogenetic data for the land planarian Dolichoplana carvalhoi. Two different karyotypes, one diploid (2n = 2x = 14 chromosomes) and one triploid (2n = 3x = 21 chromosomes), corresponding to two morphological body patterns, are described. Chromosomes from regenerating blastema were studied after routine Giemsa staining and CBG banding. Our analyses revealed heteromorphisms in chromosomes 2, 3 and 4 of the diploid karyotype and in chromosomes 1, 3 and 7 of the triploid karyotype. Further studies are needed in order to determine if the two morphological patterns of D. carvalhoi represent distinct species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Land planarians; Dolichoplana carvalhoi; Cytogenetics; Triploidy; Heteromorphisms; C-bands.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300013

Early infantile form of galactosialidosis in a female baby with a prenatal diagnosis of fetal ascites: First case in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Cláudia Maria Carvalho dos; Correia,Patrícia Santana; Rosa,Antônio Abílio Santa; Vaniazzi,Elde; Coelho,Janice Carneiro; Burin,Maira Graeff; Giugliani,Roberto; Fensom,Anthony H.; Oliveira,Cesário Paulo Honório de; Oliveira,Maria Lúcia Costa  de; Llerena Jr.,Juan Clinton.
We present the first case of an early infantile form of galactosialidosis among Brazilians. This very rare and severe lysosomal storage disease has only a dozen patients clearly diagnosed worldwide. Clinical, pathological and biochemical features were consistent with previously published findings. We detected the disorder in a 7-month-old female baby with prenatal diagnosis of ascites. Evolution of the storage disease was monitored through routine thin-layer chromatography (TLC) for urinary oligosaccharides as part of a screening program for inborn errors of metabolism (IEM) in high-risk children, carried out in Rio de Janeiro.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400005

Morphological and agronomical characterization and estimates of genetic parameters of sesbania Scop. (Leguminosae) accessions
Provedor de dados: Genetics and Molecular Biology
Autores: Veasey,E.A.; Schammass,E.A.; Vencovsky,R.; Martins,P.S.; Bandel,G.
Twenty-two accessions of seven Sesbania (Leguminosae) species: S. emerus, S. rostrata, S. tetraptera, S. exasperata (annuals), S. grandiflora, S. sesban and S. virgata (perennials), used for ruminant fodder, firewood, wood products, soil improvement, and human food, were investigated, with the aim of characterizing both inter- and intraspecific genetic variability, estimating genetic parameters for the characters evaluated and appraising the forage potential of the accessions. These were planted at the Instituto de Zootecnia, Nova Odessa, SP, Brazil, in a randomized complete block design with 22 treatments and four replications. Seventeen morphological and 17 agronomic characters were evaluated. Genetic parameters coefficient of intraspecific genetic...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100017

Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP
Provedor de dados: Genetics and Molecular Biology
Autores: Raut,Ashwin A.; Kumar,Anil; Kala,Sheo N.; Chhokar,Vinod; Rana,Neeraj; Beniwal,Vikas; Jaglan,Sundeep; Samuchiwal,Sachin K.; Singh,Jitender K.; Mishra,Anamika.
Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and DNA sequencing to examine polymorphism in the region spanning exon 7 to exon 9 of the DGAT1 gene in Murrah and Pandharpuri buffaloes. Three alleles (A, B and C) and four novel single-nucleotide polymorphisms were identified in the buffalo DGAT1 gene. The frequencies of the alleles differed between the two buffalo breeds, with allele C being present in Murrah but not in Pandharpuri buffalo. The allele variation detected in this work may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buffalo; DGAT1; PCR-SSCP; Polymorphism; SNP.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400011

Effects of artichoke (Cynara scolymus) leaf and bloom head extracts on chemically induced DNA lesions in Drosophila melanogaster
Provedor de dados: Genetics and Molecular Biology
Autores: Jacociunas,Laura Vicedo; Dihl,Rafael Rodrigues; Lehmann,Mauricio; Ferraz,Alexandre de Barros Falcão; Richter,Marc François; Silva,Juliana da; Andrade,Heloísa Helena Rodrigues de.
The genotoxicity of bloom head (BHE) and leaf (LE) extracts from artichoke (Cynara scolymus L.), and their ability to modulate the mutagenicity and recombinogenicity of two alkylating agents (ethyl methanesulfonate - EMS and mitomycin C - MMC) and the intercalating agent bleomycin (BLM), were examined using the somatic mutation and recombination test (SMART) in Drosophila melanogaster. Neither the mutagenicity nor the recombinogenicity of BLM or MMC was modified by co- or post-treatment with BHE or LE. In contrast, co-treatment with BHE significantly enhanced the EMS-induced genotoxicity involving mutagenic and/or recombinant events. Co-treatment with LE did not alter the genotoxicity of EMS whereas post-treatment with the highest dose of LE significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artichoke; Drosophila melanogaster; Recombinogenicity; SMART.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100015

Complete sequence and comparative analysis of the chloroplast genome of Plinia trunciflora
Provedor de dados: Genetics and Molecular Biology
Autores: Eguiluz,Maria; Yuyama,Priscila Mary; Guzman,Frank; Rodrigues,Nureyev Ferreira; Margis,Rogerio.
Abstract Plinia trunciflora is a Brazilian native fruit tree from the Myrtaceae family, also known as jaboticaba. This species has great potential by its fruit production. Due to the high content of essential oils in their leaves and of anthocyanins in the fruits, there is also an increasing interest by the pharmaceutical industry. Nevertheless, there are few studies focusing on its molecular biology and genetic characterization. We herein report the complete chloroplast (cp) genome of P. trunciflora using high-throughput sequencing and compare it to other previously sequenced Myrtaceae genomes. The cp genome of P. trunciflora is 159,512 bp in size, comprising inverted repeats of 26,414 bp and single-copy regions of 88,097 bp (LSC) and 18,587 bp (SSC). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jaboticaba; Myrtaceae; Chloroplast genome; Next-generation sequencing.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500871

Cytogenetic study of the genus Cousinia (Asteraceae, section Serratuloideae) in Iran
Provedor de dados: Genetics and Molecular Biology
Autores: Sheidai,Masoud; Mehdigholi,Kazem; Ghahreman,Ahmad; Attar,Farideh.
Meiotic studies of ploidy level, chromosome paring and chiasma frequency were performed on 11 Cousinia (Asteraceae) species of the section Serratuloideae. The diploid number of the species studied was 2n = 2x = 24 and 26 so these species possess two different basic numbers (x = 12 or 13), a phenomenon common to other sections of the genus. The chromosome numbers of 9 species are reported here for the first time. When the 2n = 24 and 2n = 26 species were subjected to cluster analysis based on relative meiotic characters two different clusters were formed indicating their distinctness. Our data support the results obtained from morphometry, anatomy, pollen morphology and molecular studies of the genus Cousinia.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cousinia; Chiasma frequency; Cluster analysis; Meiosis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100022

Distribution of glutathione S-transferase GSTM1 and GSTT1 null phenotypes in Brazilian Amerindians
Provedor de dados: Genetics and Molecular Biology
Autores: Klautau-Guimarães,Maria de Nazaré; Hiragi,Cássia de Oliveira; D'Ascenção,Renata Ferreira; Oliveira,Silviene Fabiana; Grisolia,Cesar Koppe; Hatagima,Ana; Ferrari,Íris.
The distribution of glutathione S-transferase (GST) GSTM1 and GSTT1 null phenotype frequencies in two Brazilian Amerindian tribes, the Munduruku tribe from Missão Cururu village (79 individuals) and the Kayabi tribe (41 individuals), was analyzed by polymerase chain reaction (PCR) amplification. The GST null phenotype frequencies for the Munduruku sample were 0% for GSTM1 and 27% for GSTT1 while for the Kayabi sample the null phenotype frequencies were 27% for GSTM1 and 29% for GSTT1. This is the first report of the absence of the GSTM1 null phenotype in any ethnic group.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Munduruku; Glutathione S-transferase; Kayabi; Null alleles.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100005

Chromosomal and morphological studies of diploid and polyploid cytotypes of Stevia rebaudiana (Bertoni) Bertoni (Eupatorieae, Asteraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Vanessa M. de; Forni-Martins,Eliana R.; Magalhães,Pedro M.; Alves,Marcos N.
In this study, we examined the chromosome number and some morphological features of strains of Stevia rebaudiana. The chromosomes were analyzed during mitosis and diakinesis, and the tetrad normality and pollen viability were also assessed. In addition, stomata and pollen were measured and some plant features were studied morphometrically. All of the strains had 2n = 22, except for two, which had 2n = 33 and 2n = 44. Pairing at diakinesis was n = 11II for all of the diploid strains, whereas the triploid and tetraploid strains had n = 11III and n = 11IV, respectively. Triploid and tetraploid plants had a lower tetrad normality rate than the diploids. All of the strains had inviable pollen. Thus, the higher the ploidy number, the greater the size of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asteraceae; Pollen; Polyploidy; Stevia rebaudiana; Stevioside; Stomata.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200015

Genome re-assignment of Arachis trinitensis (Sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut
Provedor de dados: Genetics and Molecular Biology
Autores: Robledo,Germán; Lavia,Graciela I.; Seijo,Guillermo.
The karyotype structure of Arachis trinitensis was studied by conventional Feulgen staining, CMA/DAPI banding and rDNA loci detection by fluorescence in situ hybridization (FISH) in order to establish its genome status and test the hypothesis that this species is a genome donor of cultivated peanut. Conventional staining revealed that the karyotype lacked the small "A chromosomes" characteristic of the A genome. In agreement with this, chromosomal banding showed that none of the chromosomes had the large centromeric bands expected for A chromosomes. FISH revealed one pair each of 5S and 45S rDNA loci, located in different medium-sized metacentric chromosomes. Collectively, these results suggest that A. trinitensis should be removed from the A genome and be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CMA/DAPI bands; Genome donors; Peanut genetic origin; RDNA loci.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400018

Cloning and endogenous expression of a Eucalyptus grandis UDP-glucose dehydrogenase cDNA
Provedor de dados: Genetics and Molecular Biology
Autores: Labate,Mônica T. Veneziano; Bertolo,Ana L. Ferreira; Nascimento,Daniela Defávari do; Gutmanis,Gunta; Andrade,Alexander de; Rodrigues,Maria J. Calderan; Camargo,Eduardo L.O.; Boaretto,Luis Felipe; Moon,David H.; Bragatto,Juliano; Labate,Carlos A.
UDP-glucose dehydrogenase (UGDH) catalyzes the oxidation of UDP-glucose (UDP-Glc) to UDP-glucuronate (UDP-GlcA), a key sugar nucleotide involved in the biosynthesis of plant cell wall polysaccharides. A full-length cDNA fragment coding for UGDH was cloned from the cambial region of 6-month-old E. grandis saplings by RT-PCR. The 1443-bp-ORF encodes a protein of 480 amino acids with a predicted molecular weight of 53 kDa. The recombinant protein expressed in Escherichia coli catalyzed the conversion of UDP-Glc to UDP-GlcA, confirming that the cloned cDNA encodes UGDH. The deduced amino acid sequence of the cDNA showed a high degree of identity with UGDH from several plant species. The Southern blot assay indicated that more than one copy of UGDH is present...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: UDP-glucose; UDP-glucuronate; Hemicellulose; Pectin; Cell wall.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400015

Regulation of gene expression in Mycoplasmas: contribution from Mycoplasma hyopneumoniae and Mycoplasma synoviae genome sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Madeira,Humberto Maciel França; Gabriel,Jane Eyre.
This report describes the transcription apparatus of Mycoplasma hyopneumoniae (strains J and 7448) and Mycoplasma synoviae, using a comparative genomics approach to summarize the main features related to transcription and control of gene expression in mycoplasmas. Most of the transcription-related genes present in the three strains are well conserved among mycoplasmas. Some unique aspects of transcription in mycoplasmas and the scarcity of regulatory proteins in mycoplasma genomes are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma hyopneumoniae; Mycoplasma synoviae; Transcription; Comparative genomics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200016

Genetic control of orange hilum corona of carioca beans (Phaseolus vulgaris)
Provedor de dados: Genetics and Molecular Biology
Autores: Tomaz,Juarez Pires; Moda-Cirino,Vânia; Fonseca Junior,Nelson da Silva; Ruas,Paulo Maurício.
The purpose of this research was to elucidate the genetic control of orange corona color in carioca common beans (Phaseolus vulgaris). We made four crosses between carioca group cultivars that differed in respect to the presence or absence of an orange hilum corona color. The F2, F3, F1BC11, F1BC21, F2BC11 and F2BC21 phenotypic segregations were evaluated with a chi-square test which fitted with the hypothesis that one gene with a dominant allele is responsible for the orange corona color. All generations resulting from the four different crosses showed segregation patterns which agreed with the expected proportions. Our results show that the dominant G allele controls orange corona color in the carioca bean group.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carioca bean group; Corona color; G locus; Genetic inheritance.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400016

BRCA1 mutations in Brazilian patients
Provedor de dados: Genetics and Molecular Biology
Autores: Lourenço,Juliano Javert; Vargas,Fernando R.; Bines,José; Santos,Elizete M.; Lasmar,Cezar A. P.; Costa,Célia H.; Teixeira,Eliane M. B.; Maia,Maria C. M.; Coura,Fátima; Silva,Carlos H. D.; Moreira,Miguel A. M.
BRCA1 mutations are known to be responsible for the majority of hereditary breast and ovarian cancers in women with early onset and a family history of the disease. In this paper we present a mutational survey conducted in 47 Brazilian patients with breast/ovarian cancer, selected based on age at diagnosis, family history, tumor laterality, and presence of breast cancer in male patients. All 22 coding exons and intron-exon junctions were sequenced. Constitutional mutations were found in seven families, consisting of one insertion (insC5382) in exon 20 (four patients), one four base-pair deletion (3450-3453delCAAG) in exon 11 resulting in a premature stop codon (one patient), one transition (IVS17+2T&gt; C) in intron 17 affecting a mRNA splicing site (one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BRCA1; Breast cancer; Ovarian cancer.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400006

Estimates of direct, maternal and grandmaternal genetic effects for growth traits in Gobra cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Diop,M.; Dodenhoff,J.; Van Vleck,L.D.
Estimates of genetic parameters for birth (N = 3909), weaning (N = 3425), yearling (N = 2764) and final (N = 2144) weights were obtained from the records of Gobra cattle collected at the Centre de Recherches Zootechniques de Dahra, Senegal. Three animal models were fitted to obtain estimates by REML using an average information (AI) approach. Model 1 considered random direct, maternal genetic and maternal permanent environmental effects. In model 2, a general grandmaternal effect was added to the random effects considered in model 1, and in model 3, the general grandmaternal effect was divided into grandmaternal genetic and grandmaternal permanent environmental effects. All models allowed covariances among genetic effects. The inclusion of grandmaternal...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300013

Comparative genome analysis of the SPL gene family reveals novel evolutionary features in maize
Provedor de dados: Genetics and Molecular Biology
Autores: Peng,Xiaojian; Wang,Qianqian; Zhao,Yang; Li,Xiaoyu; Ma,Qing.
Abstract SPLs are plant-specific transcription factors that play important regulatory roles in plant growth and development. Systematic analysis of the SPL family has been performed in numerous plants, such as Arabidopsis, rice, and Populus. However, no comparative analysis has been performed across different species to examine evolutionary features. In this study, we present a comparative analysis of SPLs in different species. The results showed that 84 SPLs of the four species can be divided into six groups according to phylogeny. We found that most of the SPL-containing regions in maize showed extensive conservation with duplicated regions of rice and sorghum. A gene duplication analysis in maize indicated that ZmSPLs showed a significant excess of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SPL; Phylogenetic relationship; Gene duplication; MiR156 expression.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300380

Analysis of restriction fragment length polymorphism in the kappa-casein gene related to weight expected progeny difference in Nellore cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Biase,Fernando H.; Garnero,Analia Del V.; Bezerra,Luiz A.F.; Rosa,Arthur J.M.; Lôbo,Raysildo B.; Martelli,Lúcia.
Restriction fragment length polymorphism (RFLP) has been detected at the bovine kappa-casein locus. The polymorphism has been analyzed for its effects in cattle production, mostly for milk traits and even for maternal effect on pre-weaning weights. We used polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) to genotype 408 Nellore animals for the non-silent mutation (Thr/Ile136 and Asp/Ala148) that characterizes the A and B variants of the polymorphism and compared expected progeny difference (EPD) for a maternal effect on 120 and 210 days weights and direct EPD for 120, 210, 450 and 550 day weight between AA and AB animals. The EPD values were obtained from the University of São Paulo (Brazil) Nellore Cattle Breeding Program,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Weight; Kappa-casein; Genetic marker.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100014

Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuruí Lake, in Eastern Brazilian Amazonia
Provedor de dados: Genetics and Molecular Biology
Autores: Santana,Priscila Bessa; Ghilardi Junior,Rubens; Alves,Claudio Nahum; Silva,Jeronimo Lameira; McCulloch,John Anthony; Schneider,Maria Paula Cruz; Silva,Artur da Costa da.
Methanogenic archaeans are organisms of considerable ecological and biotechnological interest that produce methane through a restricted metabolic pathway, which culminates in the reaction catalyzed by the Methyl-coenzyme M reductase (Mcr) enzyme, and results in the release of methane. Using a metagenomic approach, the gene of the a subunit of mcr (mcrα) was isolated from sediment sample from an anoxic zone, rich in decomposing organic material, obtained from the Tucuruí hydroelectric dam reservoir in eastern Brazilian Amazonia. The partial nucleotide sequences obtained were 83 to 95% similar to those available in databases, indicating a low diversity of archaeans in the reservoir. Two orders were identified -the Methanomicrobiales, and a unique Operational...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazonian; Archaea; Diversity; Methyl-coenzyme M reductase; Mcrα; Homology modeling.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100018

Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
Provedor de dados: Genetics and Molecular Biology
Autores: Rudi Emerson de Lima,Procópio; Araújo,Welington Luiz; Andreote,Fernando Dini; Azevedo,João Lúcio.
A circular cryptic plasmid named pPAGA (2,734 bp) was isolated from Pantoea agglomerans strain EGE6 (an endophytic bacterial isolate from eucalyptus). Sequence analysis revealed that the plasmid has a G+C content of 51% and contains four potential ORFs, 238(A), 250(B), 131(C), and 129(D) amino acids in length without homology to known proteins. The shuttle vector pLGM1 was constructed by combining the pPAGA plasmid with pGFPmut3.0 (which harbors a gene encoding green fluorescent protein, GFP), and the resulting construct was used to over-express GFP in E. coli and P. agglomerans cells. GFP production was used to monitor the colonization of strain EGE6gfp in various plant tissues by fluorescence microscopy. Analysis of EGE6gfp colonization showed that 14...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Green fluorescent protein; Bacteria; Host plant; Eucalyptus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100018

DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
Provedor de dados: Genetics and Molecular Biology
Autores: Sandhu,S.S.; Colombo,Carlos; Bastos,Cândido R.; Siqueira,Walter J.
Rice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines which differed from susceptible lines. By comparing different susceptible lines, nine DNA amplifications of seven primers (OPA5(1000), OPA5(1200,) OPG17(700), OPG18(850), OPG19(500), OPG19(600), OPF9(600), OPF17(1200) and OPF19(600)) were identified as dominant markers for the blast resistant gene in resistant cultivar lines. These loci facilitate the indirect scoring of blast resistant and blast susceptible genotypes. The codomine RAPDs markers will facilitate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rice; SCAR; Arbitrary primers; Pyricularia oryzae.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011

Genotoxicity of Nicotiana tabacum leaves on Helix aspersa
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Fernanda R. da; Erdtmann,Bernardo; Dalpiaz,Tiago; Nunes,Emilene; Ferraz,Alexandre; Martins,Tales L.C.; Dias,Johny F.; Rosa,Darlan P. da; Porawskie,Marilene; Bona,Silvia; Silva,Juliana da.
Tobacco farmers are routinely exposed to complex mixtures of inorganic and organic chemicals present in tobacco leaves. In this study, we examined the genotoxicity of tobacco leaves in the snail Helix aspersa as a measure of the risk to human health. DNA damage was evaluated using the micronucleus test and the Comet assay and the concentration of cytochrome P450 enzymes was estimated. Two groups of snails were studied: one fed on tobacco leaves and one fed on lettuce (Lactuca sativa L) leaves (control group). All of the snails received leaves (tobacco and lettuce leaves were the only food provided) and water ad libitum. Hemolymph cells were collected after 0, 24, 48 and 72 h. The Comet assay and micronucleus test showed that exposure to tobacco leaves for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Genotoxicity; Micronucleus test; Helix aspersa; Nicotiana tabacum; Tobacco leaves.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200018

A novel nonsense mutation, E150X, in the SOX9 gene underlying campomelic dysplasia
Provedor de dados: Genetics and Molecular Biology
Autores: Shotelersuk,Vorasuk; Jaruratanasirikul,Somchit; Sinthuwiwat,Thivaratana; Janjindamai,Waricha.
Campomelic dysplasia (CD) is an autosomal dominant skeletal malformation syndrome with features including bowed lower limbs with pretibial skin dimpling, hypoplastic scapulae and pelvic bones, and 11 pairs of ribs. Mutations in the SOX9 gene have been identified to cause CD. The gene encodes a transcription factor containing a dimerization, a high mobility group, and a C-terminal transactivation (TA) domain. Up to now, 35 SOX9 mutations have been published. In the present study, we describe a Thai girl with clinically and radiologically typical CD. Direct sequencing analysis of the PCR products for the entire coding region of SOX9 revealed that she was heterozygous for a novel 448G &gt; T in exon 2 of SOX9. The DNA change was expected to result in E150X...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SOX9; Campomelic dysplasia; Mutation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400007

A genomic approach to elucidating grass flower development
Provedor de dados: Genetics and Molecular Biology
Autores: Dornelas,Marcelo C.; Rodriguez,Adriana P.M.
In sugarcane (Saccharum sp) as with other species of grass, at a certain moment of its life cycle the vegetative meristem is converted into an inflorescence meristem which has at least two distinct inflorescence branching steps before the spikelet meristem terminates in the production of a flower (floret). In model dicotyledonous species such successive conversions of meristem identities and the concentric arrangement of floral organs in specific whorls have both been shown to be genetically controlled. Using data from the Sugarcane Expressed Sequence Tag (EST) Project (SUCEST) database, we have identified all sugarcane proteins and genes putatively involved in reproductive meristem and flower development. Sequence comparisons of known flower-related genes...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100011

The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin
Provedor de dados: Genetics and Molecular Biology
Autores: Velho,Renata Voltolini; Basso,Ana Paula; Segalin,Jeferson; Costa-Medina,Luis Fernando; Brandelli,Adriano.
This report demonstrates the usefulness of PCR for the genes spaS and sboA as a means of identifying Bacillus strains with a potential to produce subtilin and subtilosin A. One collection strain and five Bacillus spp. isolated from aquatic environments in the Amazon basin were screened by PCR using primers for sboA and spaS designed specifically for this study. The sequences of the PCR products showed elevated homology with previously described spaS and sboA genes. Antimicrobial peptides were isolated from culture supernatants and analyzed by mass spectrometry. For all samples, the mass spectra revealed clusters with peaks at m/z 3300-3500 Da, corresponding to subtilosin A, subtilin and isoforms of these peptides. These results suggest that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus; Bacteriocin; Polymerase chain reaction; Subtilin; Subtilosin A.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100014

The combined analysis as the best strategy for Dual RNA-Seq mapping
Provedor de dados: Genetics and Molecular Biology
Autores: Espindula,Eliandro; Sperb,Edilena Reis; Bach,Evelise; Passaglia,Luciane Maria Pereira.
Abstract In Dual RNA-Seq experiments the simultaneous extraction of RNA and analysis of gene expression data from both interacting organisms could be a challenge. One alternative is separating the reads during in silico data analysis. There are two main mapping methods used: sequential and combined. Here we present a combined approach in which the libraries were aligned to a concatenated genome to sort the reads before mapping them to the respective annotated genomes. A comparison of this method with the sequential analysis was performed. Two RNA-Seq libraries available in public databases consisting of a eukaryotic (Zea mays) and a prokaryotic (Herbaspirillum seropediceae) organisms were mixed to simulate a Dual RNA-Seq experiment. Libraries from real...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dual RNA-Seq; Sequential analysis; Combined analysis; Mapping strategies.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500803

TaWRKY68 responses to biotic stresses are revealed by the orthologous genes from major cereals
Provedor de dados: Genetics and Molecular Biology
Autores: Ding,Bo; Wang,Junbin; Song,Na; Li,Ming; Cheng,Qiaolin; Huang,Guozhong; Guo,Yaolin; Fu,Yang; Xie,Chaojie; Sun,Qixin; Xie,Xiaodong.
WRKY transcription factors have been extensively characterized in the past 20 years, but in wheat, studies onWRKY genes and their function are lagging behind many other species. To explore the function of wheat WRKY genes, we identified a TaWRKY68 gene from a common wheat cultivar. It encodes a protein comprising 313 amino acids which harbors 19 conserved motifs or active sites. Gene expression patterns were determined by analyzing microarray data of TaWRKY68 in wheat and of orthologous genes from maize, rice and barley using Genevestigator. TaWRKY68 orthologs were identified and clustered using DELTA-BLAST and COBALT programs available at NCBI. The results showed that these genes, which are expressed in all tissues tested, had relatively higher levels in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; TaWRKY68; Orthologous genes; Genevestigator; Gene functions.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100013

Evidence of an XX/XY sex chromosome system in the fish Dormitator maculatus (Teleostei, Eleotrididae)
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Claudio; Toledo,Lurdes Foresti de Almeida.
The fish Dormitator maculatus has a chromosomes number of 2n = 46, females having a karyotype of 14 M, 28 SM, 2 ST and 2A and males 13 M, 28 SM, 3 ST and 2A. The presence of a heteromorphic pair in the males and a corresponding homomorphic pair in the females suggest the occurrence of an XX/XY sex chromosome system in D. maculatus. The putative X chromosome has a pericentromeric C-band positive segment and the putative Y chromosome a C-band positive short arm.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish cytogenetics; Karyotypes; Ag-NORs; C-band; Sex chromosomes.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400013

Complex networks: the key to systems biology
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Luciano da F.; Rodrigues,Francisco A.; Cristino,Alexandre S.
Though introduced recently, complex networks research has grown steadily because of its potential to represent, characterize and model a wide range of intricate natural systems and phenomena. Because of the intrinsic complexity and systemic organization of life, complex networks provide a specially promising framework for systems biology investigation. The current article is an up-to-date review of the major developments related to the application of complex networks in biology, with special attention focused on the more recent literature. The main concepts and models of complex networks are presented and illustrated in an accessible fashion. Three main types of networks are covered: transcriptional regulatory networks, protein-protein interaction networks...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics; Complex networks; Metabolic networks; Protein-protein networks; Systems biology; Transcription networks.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400001

Transferability and characterization of microsatellite markers in two Neotropical Ficus species
Provedor de dados: Genetics and Molecular Biology
Autores: Nazareno,Alison Gonçalves; Pereira,Rodrigo Augusto Santinelo; Feres,Juliana Massimino; Mestriner,Moacyr Antônio; Alzate-Marin,Ana Lilia.
Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci were polymorphic when tested across 60 F. citrifolia and 60 F. eximia individuals. For F. citrifolia, there were 4 to 15 alleles per locus, whereas expected heterozygosities ranged from 0.31 to 0.91. In the case of F. eximia, this was 2 to 12 alleles per locus and expected heterozygosities from 0.42 to 0.87.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecological genetics; Ficus citrifolia; Ficus eximia; Moraceae; SSRs.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300021

The cytogenetics and evolution of forage legumes from Rio Grande do Sul: a review
Provedor de dados: Genetics and Molecular Biology
Autores: Schifino-Wittmann,Maria Teresa.
The work developed by the Cytogenetics Group of the Department of Forage Plants and Agrometeorology (Departamento de Plantas Forrageiras e Agrometerologia - DPFA), Agronomy Faculty, Federal University of Rio Grande do Sul (UFRGS), are reviewed in the present study. Topics discussed include: the chromosome numbers and meiotic behavior of Desmodium and Vigna; the application of cytogenetic methods (e.g., polyploidy induction in Trifolium riograndense) to plant breeding; the genetic control of chromosome pairing in autopolyploids of T. riograndense; karyotypes of the Vicia sativa aggregate in Southern Brazil as an example of a founder effect leading to a reduction in karyotype but not to morphological variability; data on the karyotypes of four Lathyrus...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400044

Identification of microRNA signature in different pediatric brain tumors
Provedor de dados: Genetics and Molecular Biology
Autores: Tantawy,Marwa; Elzayat,Mariam G.; Yehia,Dina; Taha,Hala.
Abstract Understanding pediatric brain tumor biology is essential to help on disease stratification, and to find novel markers for early diagnosis. MicroRNA (miRNA) expression has been linked to clinical outcomes and tumor biology. Here, we aimed to detect the expression of different miRNAs in different pediatric brain tumor subtypes to discover biomarkers for early detection and develop novel therapies. Expression of 82 miRNAs was detected in 120 pediatric brain tumors from fixed-formalin paraffin-embedded tissues, low-grade glioma, high-grade glioma, ependymoma, and medulloblastoma, using quantitative real-time PCR. Low-expression of miR-221, miR-9, and miR-181c/d and over-expression of miR-101, miR-222, miR-139, miR-1827, and miR-34c was found in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNA; Pediatric; Central nervous system tumors.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100027

A molecular marker distinguishes the subspecies Melipona quadrifasciata quadrifasciata and Melipona quadrifasciata anthidioides (Hymenoptera: Apidae, Meliponinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Waldschmidt,Ana M.; Barros,Everaldo G. de; Campos,Lucio A.O.
The stingless bee species Melipona quadrifasciata includes two subspecies, Melipona quadrifasciata anthidioids and Melipona quadrifasciata quadrifasciata. The morphological difference between the two subspecies is the presence of three to five continuous yellow stripes on the terga on the 3rd to 6th segments in workers and males of M. q. quadrifasciata, and two to five interrupted bands in M. q. anthidioides. We identified a DNA marker which is present in M. q. quadrifasciata and absent in M. q. anthidioides. Only one among the M. q. quadrifasciata colonies did not present the marker. It was also absent in bees collected in northern Minas Gerais State (Brazil), despite their morphological resemblance to M. q. quadrifasciata. The marker can be used for...
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300019

Chromosomal polymorphism and comparative chromosome painting in the rufous-collared sparrow (Zonotrichia capensis)
Provedor de dados: Genetics and Molecular Biology
Autores: Bülau,Sandra Eloisa; Kretschmer,Rafael; Gunski,Ricardo José; Garnero,Analía del Valle; O’Brien,Patricia C. M.; Ferguson-Smith,Malcolm A.; Oliveira,Edivaldo Herculano Correa de; Freitas,Thales Renato Ochotorena de.
Abstract Zonotrichia capensis is widely distributed in the Neotropics. Previous cytogenetic studies demonstrated the presence of polymorphisms in two chromosome pairs (ZCA2 and ZCA4). Here, we report results based on comparative chromosome painting, using probes derived from Gallus gallus and Leucopternis albicollis, focused on characterizing the chromosome organization of Z. capensis. Our results demonstrate the conservation of ancestral syntenies as observed previously in other species of passerine. Syntenies were rearranged by a series of inversions in the second chromosome as described in other Passeriformes, but in this species, by using probes derived from L. albicollis we observed an extra inversion in the second chromosome that had not previously...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birds; Chromosomal rearrangements; Molecular cytogenetics; FISH.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500799

Characterization of the Arachis (Leguminosae) D genome using fluorescence in situ hybridization (FISH) chromosome markers and total genome DNA hybridization
Provedor de dados: Genetics and Molecular Biology
Autores: Robledo,Germán; Seijo,Guillermo.
Chromosome markers were developed for Arachis glandulifera using fluorescence in situ hybridization (FISH) of the 5S and 45S rRNA genes and heterochromatic 4'-6-diamidino-2-phenylindole (DAPI) positive bands. We used chromosome landmarks identified by these markers to construct the first Arachis species ideogram in which all the homologous chromosomes were precisely identified. The comparison of this ideogram with those published for other Arachis species revealed very poor homeologies with all A and B genome taxa, supporting the special genome constitution (D genome) of A. glandulifera. Genomic affinities were further investigated by dot blot hybridization of biotinylated A. glandulifera total DNA to DNA from several Arachis species, the results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome markers; DAPI bands; RDNA loci; Dot blot hybridization; Genome relationships.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400019

Epistasis interaction of QTL effects as a genetic parameter influencing estimation of the genetic additive effect
Provedor de dados: Genetics and Molecular Biology
Autores: Bocianowski,Jan.
Epistasis, an additive-by-additive interaction between quantitative trait loci, has been defined as a deviation from the sum of independent effects of individual genes. Epistasis between QTLs assayed in populations segregating for an entire genome has been found at a frequency close to that expected by chance alone. Recently, epistatic effects have been considered by many researchers as important for complex traits. In order to understand the genetic control of complex traits, it is necessary to clarify additive-by-additive interactions among genes. Herein we compare estimates of a parameter connected with the additive gene action calculated on the basis of two models: a model excluding epistasis and a model with additive-by-additive interaction effects....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Additive gene action effect; Barley; Doubled haploid lines; Epistasis; QTL.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100013

Genetic diversity of Toll-like receptor 5 among pig populations
Provedor de dados: Genetics and Molecular Biology
Autores: Yang,Xiuqin; Li,Haitao; Guan,Qingzhi; Liu,Di.
The Toll-like receptor 5 (TLR5) recognizes flagellin of Gram-positive and -negative bacteria and plays an important role in the host defense system. Here, we surveyed single nucleotide polymorphisms (SNPs) in the coding sequence of the porcine TLR5 gene in 83 individuals from five pig breeds, these including Chinese local populations and Western commercial pig breeds. A total of 19 medium polymorphic SNPs (0.25 &lt; PIC &lt; 0.5) were identified, three of which were missense mutations that clustered within the extracellular domain of TLR5. One of the non-synonymous SNPs fell within a 228-amino acid region which has been shown to be important for flagellin recognition. Four SNPs were only found with high frequencies in Oriental pig breeds. The 19 SNPs were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Porcine; TLR5; SNPs; Haplotype.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100006

Optimization of PCR amplification of maize microsatellite loci
Provedor de dados: Genetics and Molecular Biology
Autores: Ogliari,Juliana Bernardi; Boscariol,Raquel L.; Camargo,Luis E.A.
Maize (Zea mays L.) microsatellite loci are useful as genetic markers because they are numerous, occur in every chromosome, and have a high content of polymorphism information. Furthermore, they can be amplified by PCR and the resulting fragments resolved on agarose gels. A major problem, however, is that the primers used in their amplification often have different length and nucleic acid content, requiring optimization of PCR amplification programs for each locus. We developed "touchdown" PCR programs successfully used in the amplification of a set of 125-maize microsatellite loci, chosen as representative of all chromosomes. The programs varied both in relation to the annealing temperature (Tm) and primer concentration. Primers could be divided into four...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200026

Intrachromosomal karyotype asymmetry in Orchidaceae
Provedor de dados: Genetics and Molecular Biology
Autores: Medeiros-Neto,Enoque; Nollet,Felipe; Moraes,Ana Paula; Felix,Leonardo P.
Abstract The asymmetry indexes have helped cytotaxonomists to interpret and classify plant karyotypes for species delimitation efforts. However, there is no consensus about the best method to calculate the intrachromosomal asymmetry. The present study aimed to compare different intrachromosomal asymmetry indexes in order to indicate which are more efficient for the estimation of asymmetry in different groups of orchids. Besides, we aimed to compare our results with the Orchidaceae phylogenetic proposal to test the hypothesis of Stebbins (1971). Through a literature review, karyotypes were selected and analyzed comparatively with ideal karyotypes in a cluster analysis. All karyotypes showed some level of interchromosomal asymmetry, ranging from slightly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asymmetry index; Karyotype symmetry; Chromosome; Cytogenetic; Evolution.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400610

Susceptibility to DNA damage in workers occupationally exposed to pesticides, to tannery chemicals and to coal dust during mining
Provedor de dados: Genetics and Molecular Biology
Autores: Kvitko,Katia; Bandinelli,Eliane; Henriques,João A.P.; Heuser,Vanina D.; Rohr,Paula; Silva,Fernanda R. da; Schneider,Naye Balzan; Fernandes,Simone; Ancines,Camile; Silva,Juliana da.
Our mutagenesis group has been studying with important economic drivers of our state, such as agriculture, the footwear and leather industry and open-cast coal mining. Working conditions in these sectors have potentially harmful to humans. The aim of these studies is to determine the health risk of workers by biomonitoring subjects exposed to genotoxic agents. The main results of our studies with vineyard farmers we observed a high rate of MN and DNA damage in individuals exposed to pesticides (p &lt; 0.001). In addition, some effects of genetic polymorphisms in the modulation of MN results were observed in this group. Tobacco farmers were also evaluated at different crop times. The results showed a significant increase in the Damage index and frequency in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Occupational exposure; Comet assay; Micronucleus test; Pesticide; Coal dust.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600022

Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands
Provedor de dados: Genetics and Molecular Biology
Autores: Franco,Maurício Machaim; Antunes,Robson Carlos; Oliveira,Katiana Mello de; Pereira,Cícero Donizete; Biase,Fernando Henrique; Nunes,Francis de Morais Franco; Goulart,Luiz Ricardo.
Fourty-six non-castrated, halothane-free, male Landrace pigs were genotyped by PCR-RFLP for the Rsa I polymorphism in the PIT1 gene and classified into AA and AB genotypes. Total RNA was extracted from the pituitaries and the relative quantities of growth hormone (GH) mRNA were determined by semi-quantitative RT-PCR. Pigs with the AB genotype had higher levels of GH mRNA than those with the AA genotype (p = 0.034; Kruskal-Wallis test). This result suggests that the Rsa I polymorphism may be involved in Pit-1 protein expression or function, which in turn may influence GH transcription and expression. Thus, the Rsa I PIT1 gene polymorphism in this pig line may be used as a molecular marker to identify higher GH expression and possibly select for carcass and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PIT1 gene; Growth hormone mRNA; Semi-quantitative RT-PCR; Pig.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100003

High levels of genetic differentiation and selfing in the Brazilian cerrado fruit tree Dipteryx alata Vog. (Fabaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Tarazi,Roberto; Moreno,Maria Andréia; Gandara,Flávio Bertin; Ferraz,Elza Martins; Moraes,Mário Luiz Teixeira; Vinson,Christina Cleo; Ciampi,Ana Yamaguishi; Vencovsky,Roland; Kageyama,Paulo Yoshio.
Dipteryx alata is a native fruit tree species of the cerrado (Brazilian savanna) that has great economic potential because of its multiple uses. Knowledge of how the genetic variability of this species is organized within and among populations would be useful for genetic conservation and breeding programs. We used nine simple sequence repeat (SSR) primers developed for Dipteryx odorata to evaluate the genetic structure of three populations of D. alata located in central Brazil based on a leaf sample analysis from 101 adults. The outcrossing rate was evaluated using 300 open-pollinated offspring from 25 seed-trees. Pollen dispersal was measured by parentage analysis. We used spatial genetic structure (SGS) to test the minimal distance for harvesting seeds...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dipteryx alata; Genetic structure; Mating system; SSR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100015

Effect of soybean ureases on seed germination and plant development
Provedor de dados: Genetics and Molecular Biology
Autores: Rechenmacher,Ciliana; Wiebke-Strohm,Beatriz; Oliveira-Busatto,Luisa A. de; Polacco,Joseph C.; Carlini,Célia R.; Bodanese-Zanettini,Maria H.
Abstract Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes the putative protein product SBU-III. The present study aimed to evaluate the contribution of soybean ureases to seed germination and plant development. Analyses were performed using Eu1/Eu4/Eu5-co-suppressed transgenic plants and mutants of the Eu1 and Eu4 urease structural genes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant development; Functional study; Transgenic plants; Mutants; Urease.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200209

Phylogenomic and single nucleotide polymorphism analyses revealed the hybrid origin of Spondias bahiensis (family Anacardiaceae): de novo genome sequencing and comparative genomics
Provedor de dados: Genetics and Molecular Biology
Autores: Nobre,Lydayanne Lilás de Melo; Santos,José Daniel Oliveira dos; Leite,Rychard; Almeida,Cícero.
Abstract The genus Spondias (family Anacardiaceae) comprises 19 taxa, ten of which occur in Neotropical regions. Spondias bahiensis has been suggested to be a hybrid, although initial evidence does not support this hypothesis. The aim of this study was to test the hypothesis of the hybrid origin of S. bahiensis using high-throughput sequencing with single nucleotide polymorphism (SNP) analysis, characterization of intragenomic nuclear ribosomal DNA (nrDNA), and nuclear and chloroplast phylogenomic analyses. The SNP analysis revealed a high number of SNPs in the S. bahiensis genome, and with respect to nrDNA, S. bahiensis shared approximately half of the SNP alleles with S. tuberosa, but not with S. mombin. Combining the SNP analysis with nrDNA phylogeny...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RDNA; Phylogenetics; Evolution; Hybrid offspring.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500878

Patterns of expression of cell wall related genes in sugarcane
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,D.U.; Santos,H.P.; Tiné,M.A.; Molle,F.R.D.; Buckeridge,M.S.
Our search for genes related to cell wall metabolism in the sugarcane expressed sequence tag (SUCEST) database (&lt;A HREF="http://sucest.lbi.dcc.unicamp.br/"&gt;http://sucest.lbi.dcc.unicamp.br&lt;/A&gt;) resulted in 3,283 reads (1% of the total reads) which were grouped into 459 clusters (potential genes) with an average of 7.1 reads per cluster. To more clearly display our correlation coefficients, we constructed surface maps which we used to investigate the relationship between cell wall genes and the sugarcane tissues libraries from which they came. The only significant correlations that we found between cell wall genes and/or their expression within particular libraries were neutral or synergetic. Genes related to cellulose biosynthesis were from the...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100026

Comparative analysis of differentially expressed sequence tags of sweet orange and mandarin infected with Xylella fastidiosa
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Campos,Magnólia A.; Teixeira,Juliana E.C.; Targon,Maria Luísa P.N.; Carlos,Eduardo F.; Ravasi,Juliano F.; Fischer,Carlos N.; Machado,Marcos A.
The Citrus ESTs Sequencing Project (CitEST) conducted at Centro APTA Citros Sylvio Moreira/IAC has identified and catalogued ESTs representing a set of citrus genes expressed under relevant stress responses, including diseases such as citrus variegated chlorosis (CVC), caused by Xylella fastidiosa. All sweet orange (Citrus sinensis L. Osb.) varieties are susceptible to X. fastidiosa. On the other hand, mandarins (C. reticulata Blanco) are considered tolerant or resistant to the disease, although the bacterium can be sporadically detected within the trees, but no disease symptoms or economic losses are observed. To study their genetic responses to the presence of X. fastidiosa, we have compared EST libraries of leaf tissue of sweet orange Pêra IAC (highly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ESTs; Citrus variegated chlorosis; Biotic stress; CitEST; Phytopathogen.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500024

Expressed citrus genome: integration is the challenge
Provedor de dados: Genetics and Molecular Biology
Autores: Machado,Marcos Antônio.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500001

Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markers
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Adailton T. da; Penna,Júlio C.V.; Goulart,Luiz R.; Santos,Maria A. dos; Arantes,Neylson E.
Heterodera glycines Ichinohe, the soybean cyst nematode (SCN) was detected in Brazil in 1992 and since then it has been causing losses to Brazilian soybean crop yields. SCN populations have great genetic diversity which makes it difficult to manage this disease. The objectives of this research were to investigate the genetic variation of 16 SCN populations sampled in middle western and southeastern Brazil, utilizing random amplified polymorphic DNA (RAPD) techniques, and to establish useful and specific RAPD markers for SCN race 3. RAPD markers demonstrated genetic variability among and within SCN populations, and they could be used for monitoring nematode population dynamics. The OPA-07 primer was a reliable molecular marker for race 3, while...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200014

Physical map and one-megabase sequencing of the human immunoglobulin lambda locus
Provedor de dados: Genetics and Molecular Biology
Autores: Passos Jr.,Geraldo A.S.
The human immunoglobulin lambda (IGL) locus is located on chromosome 22q11.1-q11.2 and contains the genes responsible for the immunoglobulin lambda light chains. This locus was recently mapped (physical map) and its 1-Mb DNA totally sequenced. In this review we focus on the characterization of the v-lambda genes, its chromosomal location, genomics and sequencing of the IGL locus.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200019

Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
Provedor de dados: Genetics and Molecular Biology
Autores: Sousa,Cristina Soares de; Serrão,José Eduardo; Bonetti,Ana Maria; Amaral,Isabel Marques Rodrigues; Kerr,Warwick Estevam; Maranhão,Andréa Queiroz; Ueira-Vieira,Carlos.
The insect fat body is a multifunctional organ analogous to the vertebrate liver. The fat body is involved in the metabolism of juvenile hormone, regulation of environmental stress, production of immunity regulator-like proteins in cells and protein storage. However, very little is known about the molecular mechanisms involved in fat body physiology in stingless bees. In this study, we analyzed the transcriptome of the fat body from the stingless bee Melipona scutellaris. In silico analysis of a set of cDNA library sequences yielded 1728 expressed sequence tags (ESTs) and 997 high-quality sequences that were assembled into 29 contigs and 117 singlets. The BLAST X tool showed that 86% of the ESTs shared similarity with Apis mellifera (honeybee) genes. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fat body; Gene expression; Melipona; Stingless bees; Transcriptome.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200022

Novel sequence variations in LAMA2 and SGCG genes modulating cis-acting regulatory elements and RNA secondary structure
Provedor de dados: Genetics and Molecular Biology
Autores: Siala,Olfa; Salem,Ikhlass Hadj; Tlili,Abdelaziz; Ammar,Imen; Belguith,Hanen; Fakhfakh,Faiza.
In this study, we detected new sequence variations in LAMA2 and SGCG genes in 5 ethnic populations, and analysed their effect on enhancer composition and mRNA structure. PCR amplification and DNA sequencing were performed and followed by bioinformatics analyses using ESEfinder as well as MFOLD software. We found 3 novel sequence variations in the LAMA2 (c.3174+22_23insAT and c.6085 +12delA) and SGCG (c.*102A/C) genes. These variations were present in 210 tested healthy controls from Tunisian, Moroccan, Algerian, Lebanese and French populations suggesting that they represent novel polymorphisms within LAMA2 and SGCG genes sequences. ESEfinder showed that the c.*102A/C substitution created a new exon splicing enhancer in the 3'UTR of SGCG genes, whereas the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscular dystrophies; Exon splicing enhancer; RNA fold; SR proteins.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100032

DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Patrícia Santos Pereira; Molffeta,Greice Andreoti; Araujo,Amélia Góes de; Zago,Marco Antônio; Silva Jr.,Wilson Araújo da.
The aim of this work was to evaluate the methylation profile of the p15 (CDKN2B) gene in Brazilian patients with leukemia and to correlate the CDKN2B gene expression with the percentage of methylated CpG dinucleotides in its promoter region. Thirty-one samples from six patients with acute lymphocytic leukemia (ALL), four with chronic myeloid leukemia (CML), and 21 with acute myeloid leukemia (AML) were evaluated by MSP (Methylation-Specific PCR). The CDKN2B gene was found to be methylated in four (67%) of the six ALL samples and in 16 (76%) of the 21 AML samples, but in none of the four CML samples analyzed. We observed a correlation between the CDKN2B mRNA expression (RT-PCR) and the percentage of methylated CpG dinucleotides. Therefore, this study in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CpG island; DNA methylation; CDKN2B.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400005

Karyotypic analyses and morphological comments on the endemic and endangered Brazilian painted tree rat Callistomys pictus (Rodentia, Echimyidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Ventura,Karen; Ximenes,Gilson Evaristo Iack; Pardini,Renata; Sousa,Marcos A. Nóbrega de; Yonenaga-Yassuda,Yatiyo; Silva,Maria José de J.
The genus Callistomys belongs to the rodent family Echimyidae, subfamily Echimyinae, and its only living representative is Callistomys pictus, a rare and vulnerable endemic species of the state of Bahia, Brazil. Callistomys has been previously classified as Nelomys, Loncheres, Isothrix and Echimys. In this paper we present the karyotype of Callistomys pictus, including CBG and GTG-banding patterns and silver staining of the nucleolus organizer regions (Ag-NORs). Comments on Callistomys pictus morphological traits and a compilation of Echimyinae chromosomal data are also included. Our analyses revealed that Callistomys can be recognized both by its distintinctive morphology and by its karyotype.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Callistomys; Karyotype; Banding patterns; Echimyinae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400016

Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor
Provedor de dados: Genetics and Molecular Biology
Autores: Li,Hui-Liang; Guo,Dong; Peng,Shi-Qing.
The cDNA encoding the R1-MYB transcription factor, designated as JcR1MYB1, was isolated from Jatropha curcas using rapid amplification of cDNA ends. JcR1MYB1 contains a 951 bp open reading frame that encodes 316 amino acids. The deduced JcR1MYB1 protein was predicted to possess the conserved, 56-amino acid-long DNA-binding domain, which consists of a single helix-turn-helix module and usually occurs in R1-MYBs. JcR1MYB1 is a member of the R1-MYB transcription factor subfamily. A subcellular localization study confirmed the nuclear localization of JcR1MYB1. Expression analysis showed that JcR1MYB1 transcripts accumulated in various examined tissues, with high expression levels in the root and low levels in the stem. JcR1MYB1 transcription was up-regulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic stress; Gene expression; Jatropha curcas; R1-MYB transcription factor.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400011

Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci
Provedor de dados: Genetics and Molecular Biology
Autores: Pinto,Luciana Rossini; Vieira,Maria Lucia Carneiro; Souza Jr.,Claudio Lopes de; Souza,Anete Pereira de.
A modified reciprocal recurrent selection (RRS) method, which employed one cycle of high-intensity selection, was applied to two tropical maize (Zea mays L.) populations, BR-105 and BR-106, originating the improved synthetics IG-3 and IG-4, respectively. In the present study the effects of this kind of selection on the genetic structure of these populations and their synthetics were investigated at 30 microsatellite (SSR) loci. A total of 125 alleles were revealed. A reduction in the number of alleles was observed after selection, as well as changes in allele frequencies. In nearly 13% (BR-105) and 7% (BR-106) of the loci evaluated, the changes in allele frequencies were not explained, exclusively due to the effects of genetic drift. The effective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population genetics; Plant breeding; Molecular markers.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300022

Analysis by RAPD of the genetic structure of Astyanax altiparanae (Pisces, Characiformes) in reservoirs on the Paranapanema River, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Leuzzi,Maria Sueli Papa; Almeida,Fernanda Simões de; Orsi,Mário Luís; Sodré,Leda Maria Koelblinger.
In this study, the RAPD technique was used to analyze the genetic structure of populations of the fish Astyanax altiparanae (Characidae, Tetragonopterinae) living in the lower, middle and upper Paranapanema River, Brazil. The aim was to assess this structure regarding fish handling and conservation programs. The genetic variability (P) was found to be 42.64%, 75% and 75% in the low, middle and upper reaches, respectively. The dendrogram of genetic similarity, obtained by comparative analysis of the sets of samples from the three sites, showed the formation of three clusters. All of the genetic parameters used indicate that the population in the lower Paranapanema is genetically different from those in the middle and upper sections. The theta P test shows...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astyanax altiparanae; RAPD; Genetic structure; Genetic conservation; Fish.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300009

In silico survey of resistance (R) genes in Eucalyptus transcriptome
Provedor de dados: Genetics and Molecular Biology
Autores: Barbosa-da-Silva,Adriano; Wanderley-Nogueira,Ana C.; Silva,Raphaela R.M.; Berlarmino,Luiz C.; Soares-Cavalcanti,Nina M.; Benko-Iseppon,Ana M.
A major goal of plant genome research is to recognize genes responsible for important traits. Resistance genes are among the most important gene classes for plant breeding purposes being responsible for the specific immune response including pathogen recognition, and activation of plant defence mechanisms. These genes are quite abundant in higher plants, with 210 clusters found in Eucalyptus FOREST database presenting significant homology to known R-genes. All five gene classes of R-genes with their respective conserved domains are present and expressed in Eucalyptus. Most clusters identified (93) belong to the LRR-NBS-TIR (genes with three domains: Leucine-rich-repeat, Nucleotide-binding-site and Toll interleucine 1-receptor), followed by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serine-threonine kinase; Nucleotide binding site; Leucine-rich repeats; Gene-for-gene interaction.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400011

Sequence variation in the melanocortin-1 receptor (MC1R) pigmentation gene and its role in the cryptic coloration of two South American sand lizards
Provedor de dados: Genetics and Molecular Biology
Autores: Corso,Josmael; Gonçalves,Gislene L.; Freitas,Thales R.O. de.
In reptiles, dorsal body darkness often varies with substrate color or temperature environment, and is generally presumed to be an adaptation for crypsis or thermoregulation. However, the genetic basis of pigmentation is poorly known in this group. In this study we analyzed the coding region of the melanocortin-1-receptor (MC1R) gene, and therefore its role underlying the dorsal color variation in two sympatric species of sand lizards (Liolaemus) that inhabit the southeastern coast of South America: L. occipitalis and L. arambarensis. The first is light-colored and occupies aeolic pale sand dunes, while the second is brownish and lives in a darker sandy habitat. We sequenced 630 base pairs of MC1R in both species. In total, 12 nucleotide polymorphisms were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adaptation; Body color; Crypsis; Liolaemidae; Pigmentation genes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100011

Genetic variation of wild and hatchery populations of the catla Indian major carp (Catla catla Hamilton 1822: Cypriniformes, Cyprinidae) revealed by RAPD markers
Provedor de dados: Genetics and Molecular Biology
Autores: Rahman,S.M. Zakiur; Khan,Mukhlesur Rahman; Islam,Shahidul; Alam,Samsul.
Genetic variation is a key component for improving a stock through selective breeding programs. Randomly amplified polymorphic DNA (RAPD) markers were used to assess genetic variation in three wild population of the catla carp (Catla catla Hamilton 1822) in the Halda, Jamuna and Padma rivers and one hatchery population in Bangladesh. Five decamer random primers were used to amplify RAPD markers from 30 fish from each population. Thirty of the 55 scorable bands were polymorphic, indicating some degree of genetic variation in all the populations. The proportion of polymorphic loci and gene diversity values reflected a relatively higher level of genetic variation in the Halda population. Sixteen of the 30 polymorphic loci showed a significant (p &lt; 0.05, p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catla catla; Genetic variation; RAPD markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100030

DNA barcoding of Brazilian sea turtles (Testudines)
Provedor de dados: Genetics and Molecular Biology
Autores: Vargas,Sarah M.; Araújo,Flávia C.F.; Santos,Fabrício R.
Five out of the seven recognized species of sea turtles (Testudines) occur on the Brazilian coast. The Barcode Initiative is an effort to undertake a molecular inventory of Earth biodiversity. Cytochrome Oxidase c subunit I (COI) molecular tags for sea turtle species have not yet been described. In this study, COI sequences for the five species of sea turtles that occur in Brazil were generated. These presented widely divergent haplotypes. All observed values were on the same range as those already described for other animal groups: the overall mean distance was 8.2%, the mean distance between families (Dermochelyidae and Cheloniidae) 11.7%, the mean intraspecific divergence 0.34%, and the mean distance within Cheloniidae 6.4%, this being 19-fold higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Barcodes; Sea turtles; Cytochrome Oxidase c subunit I (COI).
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300026

Anticlastogenic effect of vitamin C on cisplatin in vivo
Provedor de dados: Genetics and Molecular Biology
Autores: Antunes,Lusânia M. Greggi; Darin,Joana D.C.; Bianchi,Maria de Lourdes P.
The ability of vitamin C (VC) to protect against the clastogenic action of the chemotherapeutic agent cisplatin (DDP, cis-diamminedichloroplatinun II) in rat bone marrow cells was evaluated. DDP was administered to Wistar rats either alone or after treatment with VC. The rats were treated with VC (50, 100 or 200 mg/kg body weight) by gavage 10 min before the administration of DDP (5 mg/kg body weight, ip) and then sacrificed 24 h after treatment. VC significantly reduced (by about 70%) the clastogenicity of DDP in rat bone marrow cells. The antioxidant action of VC presumably modulates the clastogenic action of DDP.
Tipo: Info:eu-repo/semantics/other
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300022

DNA testing for parentage verification in a conservation nucleus of Pantaneiro horse
Provedor de dados: Genetics and Molecular Biology
Autores: Sereno,Fabiana Tavares Pires de Souza; Sereno,José Robson Bezerra; Vega-Pla,Jose Luis; Delgado,Juan Vicente.
We investigated the genealogy of the in situ conservation nucleus of the Pantaneiro horse using DNA microsatellites by evaluating 101 horses, the group consisting of 71 adult horses (3 stallions, 40 male and 31 mares) and 27 foals (14 colts and 13 fillies). Genomic DNA was extracted from hair roots and genotyped using 12 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, HMS3 HMS6, HMS7, HTG4, HTG10, LEX33 and VHL20). The number of alleles per locus varied from 6 to 13, with a mean of 7.8 and the expected heterozygosity ranged from 0.544 to 0.734 (mean 0.644). The VLH20, ASB2, HTG10, ASB23 markers had a high (&gt; 0.8) polymorphism information content and the total exclusion probability of the 12 microsatellite loci was 0.99. The genealogical study of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pantaneiro horse; Animal conservation; Microsatellites; Markers; Parentage verification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100013

No clastogenic activity of Caesalpinia ferrea Mart. (Leguminosae) extract on bone marrow cells of Wistar rats
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Andréa Bêcco de; Souza,Lilian Mara Silva; Carvalho,José Carlos Tavares; Maistro,Edson Luis.
Extracts of the fruits of Caesalpinia ferrea Mart. (Leguminosae) are widely consumed in folkloric medicine in Brazil and several other countries without any genetic toxicity evaluation. In this study we investigated, the clastogenic and cytotoxic potential of the crude aqueous extract of the fruits of C. ferrea in Wistar rat bone marrow cells using the micronucleus and chromosomal aberration test systems. The animals were treated by gavage with 3 concentrations of the extract, 500, 1000 and 1500 mg/kg, and cyclophosphamide 30 mg/kg. Bone marrow cells were collected 24 h after the treatment. There was no statistically significant difference in the mean of micronucleated polychromatic erythrocytes (MNPCE), mean number of chromosomal aberrations or mitotic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caesalpinia ferrea; Micronucleus test; Chromosome aberrations.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200028

Cytogenetic analysis and chromosomal characteristics of the polymorphic 18S rDNA in the fish Prochilodus lineatus (Characiformes, Prochilodontidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Vicari,Marcelo Ricardo; Almeida,Mara Cristina de; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando; Artoni,Roberto Ferreira.
We used differential staining techniques (BSG, GTG, AgNO3, DAPI and CMA3 banding) and fluorescent in situ hybridization (FISH) with 5S and 18S probes to investigated the karyotypic and cytogenetic chracteristics of Prochilodus lineatus specimens from a population in Vila Velha state park (Parque Estadual de Vila Velha, Ponta Grossa, Paraná state, southern Brazil). The specimens studied showed the same karyotype as that found in other P. lineatus populations, i.e. 2n = 54 biarmed chromosomes (40m + 14 sm) and c-positive heterochromatin preferentially located pericentromerically in all chromosomes. The presence of partial or totally heterochromatic supernumerary chromosomes with numeric intra-individual variation was confirmed in the analyzed population. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish cytogenetics; Prochilodus; Karyotype; Minor and major rDNA; NOR polymorphism.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400008

Effects of turmeric and its active principle, curcumin, on bleomycin-induced chromosome aberrations in Chinese hamster ovary cells
Provedor de dados: Genetics and Molecular Biology
Autores: Araújo,Maria Cristina P.; Dias,Francisca da Luz; Kronka,Sergio N.; Takahashi,Catarina S.
Naturally occurring antioxidants have been extensively studied for their capacity to protect organisms and cells from oxidative damage. Many plant constituents including turmeric and curcumin appear to be potent antimutagens and antioxidants. The effects of turmeric and curcumin on chromosomal aberration frequencies induced by the radiomimetic agent bleomycin (BLM) were investigated in Chinese hamster ovary (CHO) cells. Three concentrations of each drug, turmeric (100, 250 and 500 &lt;FONT FACE="Symbol"&gt;m&lt;/FONT&gt;g/ml) and curcumin (2.5, 5 and 10 &lt;FONT FACE="Symbol"&gt;m&lt;/FONT&gt;g/ml), were combined with BLM (10 &lt;FONT FACE="Symbol"&gt;m&lt;/FONT&gt;g/ml) in CHO cells treated during the G1/S, S or G2/S phases of the cell cycle. Neither...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300021

Genetic relatedness between cassava (Manihot esculenta Crantz) and M. flabellifolia and M. Peruviana based on both RAPD and AFLP markers
Provedor de dados: Genetics and Molecular Biology
Autores: Colombo,Carlos; Second,Gérard; Charrier,André.
The taxonomy of the genus Manihot is still uncertain and the genetic origin of cassava (M. esculenta Crantz) continues to be controversial. We studied the degree of genetic relatedness between cassava and two naturally occurring species (M. flabellifolia and M. peruviana) which are probably involved in the evolution of cassava, using RAPD and AFLP molecular markers. Thirty-three clonal accessions of cassava of known genetic diversity and 15 accessions of the wild species M. flabellifolia and M. peruviana were analyzed using 92 polymorphic RAPD bands and 73 polymorphic AFLP bands. The genetic markers were unable to differentiate the two wild species, which confirms their botanical similarity. Half of the total number of amplified bands were monomorphic in...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200030

Mating system in a neotropical tree species, Senna multijuga (Fabaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Ribeiro,Renata Acácio; Lovato,Maria Bernadete.
Senna multijuga is a pioneer tropical tree species that occurs mainly in the Brazilian Atlantic forest. We investigated the mating system of two populations of S. multijuga, one located in a reserve area (RD1) and the other (RD2) about 15 km away. The mating system parameters were estimated using the mixed mating model (software MLTR). The two populations had significantly different outcrossing rates, with population RD2 having a high rate (t m = 0.838) and population RD1, a lower rate (t m = 0.540). The values of t s were different between the two populations and also lower than those of t m. Significant t m - t s estimates indicated that biparental inbreeding contributed to the apparent selfing rate in these populations. The correlation of paternity was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isozymes; Mating systems; Neotropical tree; Senna multijuga.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300018

Molecular evidence of two cryptic species of Stramonita (Mollusca, Muricidae) in the southeastern Atlantic coast of Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: De Biasi,Juliana Beltramin; Tomás,Acácio Ribeiro Gomes; Hilsdorf,Alexandre Wagner Silva.
Abstract Snails of the genus Stramonita are commonly found in the rocky intertidal habitat of the western Atlantic Ocean coast. They belong to a monophyletic taxon that occurs along the tropical and warm-temperate Atlantic and eastern Pacific rocky shores. This genus comprises different valid species and members of the S. haemastoma complex. In the present study, samples of Stramonita were collected from three different regions of southeastern Brazil. Partial sequences of two mitochondrial genes, COI and 16S rRNA, were used to compare nucleotides sequences between Stramonita specimens. Levels of nucleotide divergence greater than 2% across the three sampled regions were used for differentiation at the species level. One of the identified species was S....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: COI; 16s rRNA; Mitochondrial DNA; Southern oyster drill; Brazilian coast.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300392

Molecular data highlight hybridization in squirrel monkeys (Saimiri, Cebidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Carneiro,Jeferson; Rodrigues-Filho,Luis Fernando da Silva; Schneider,Horacio; Sampaio,Iracilda.
Abstract Hybridization has been reported increasingly frequently in recent years, fueling the debate on its role in the evolutionary history of species. Some studies have shown that hybridization is very common in captive New World primates, and hybrid offspring have phenotypes and physiological responses distinct from those of the "pure" parents, due to gene introgression. Here we used the TA15 Alu insertion to investigate hybridization in the genus Saimiri. Our results indicate the hybridization of Saimiri boliviensis peruviensis with S. sciureus macrodon, and S. b. boliviensis with S. ustus. Unexpectedly, some hybrids of both S. boliviensis peruviensis and S. b. boliviensis were homozygous for the absence of the insertion, which indicates that the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Saimiri; Squirrel monkeys; Alu elements; Hybridization.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400539

PENCALC: a program for penetrance estimation in autosomal dominant diseases
Provedor de dados: Genetics and Molecular Biology
Autores: Horimoto,Andréa R.V. Russo; Onodera,Márcio T.; Otto,Paulo A.
We present a computer program developed for estimating penetrance rates in autosomal dominant diseases by means of family kinship and phenotype information contained within the pedigrees. The program also determines the exact 95% credibility interval for the penetrance estimate. Both executable (PenCalc for Windows) and web versions (PenCalcWeb) of the software are available. The web version enables further calculations, such as heterozygosity probabilities and assessment of offspring risks for all individuals in the pedigrees. Both programs can be accessed and down-loaded freely at the home-page address http://www.ib.usp.br/~otto/software.htm.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penetrance (rate; Value) computer program maximum likelihood estimation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300012

Patterns of ribosomal DNA distribution in hylid frogs from the  Hypsiboas faber  and  H. semilineatus  species groups
Provedor de dados: Genetics and Molecular Biology
Autores: Nunes,Rosana dos Reis Abrante; Fagundes,Valéria.
We performed a comparative analysis of the nucleolus organizer region (NOR) distribution in the karyotypes of hylid frogs from two different taxonomic groups,  Hypsiboas faber  and  H. semilineatus.  Silver nitrate staining of NORs (Ag-NORs) and fluorescence  in situ  hybridization (FISH) with a rDNA probe were used to investigate the chromosomal location of rDNA loci in two species. The karyotype of  H. semilineatus  and the Ag-NORs distribution of the four species are presented herein for the first time. After conventional staining, the four species presented very similar karyotypes with 2n = 24, but Ag-NORs analyses revealed species-specific characteristics.  H. albomarginatus, H. faber  and  H. semilineatus  had one pair of interstitial Ag-NORs in the...
Palavras-chave: Hylids; Hypsiboas; Karyotypes; FISH; Ag-NORs; Hypsiboas semilineatus; H. faber.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500028

Genetic characterization of 12 heterologous microsatellite markers for the giant tropical tree Cariniana legalis (Lecythidaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Guidugli,Marcela Corbo; Accoroni,Klaus Alvaro Guerrieri; Mestriner,Moacyr Antonio; Contel,Eucleia Primo Betioli; Martinez,Carlos Alberto; Alzate-Marin,Ana Lilia.
Twelve microsatellite loci previously developed in the tropical tree Cariniana estrellensis were genetically characterized in Cariniana legalis. Polymorphisms were assessed in 28 C. legalis individuals found between the Pardo and Mogi-Guaçu River basins in the state of São Paulo, Brazil. Of the 12 loci, 10 were polymorphic and exhibited Mendelian inheritance. The allelic richness at each locus ranged from 2-11, with an average of 7 alleles per locus, and the expected heterozygosity ranged from 0.07-0.88. These loci showed a high probability of paternity exclusion. The characteristics of these heterologous microsatellite markers indicate that they are suitable tools for investigating questions concerning population genetics in C. legalis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Jequitibá rosa; Population genetics; SSR markers; Transferability.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100022

Several pathways of hydrogen peroxide action that damage the E. coli genome
Provedor de dados: Genetics and Molecular Biology
Autores: Asad,Nasser Ribeiro; Asad,Lidia Maria Buarque Oliveira; Almeida,Carlos Eduardo Bonacossa de; Felzenszwalb,Israel; Cabral-Neto,Januário Bispo; Leitão,Alvaro Costa.
Hydrogen peroxide is an important reactive oxygen species (ROS) that arises either during the aerobic respiration process or as a by-product of water radiolysis after exposure to ionizing radiation. The reaction of hydrogen peroxide with transition metals imposes on cells an oxidative stress condition that can result in damage to cell components such as proteins, lipids and principally to DNA, leading to mutagenesis and cell death. Escherichia coli cells are able to deal with these adverse events via DNA repair mechanisms, which enable them to recover their genome integrity. These include base excision repair (BER), nucleotide excision repair (NER) and recombinational repair. Other important defense mechanisms present in Escherichia coli are OxyR and SosRS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrogen peroxide; Cross-adaptive response; Lethal synergism; Copper and iron.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200026

In silico analysis of phytohormone metabolism and communication pathways in citrus transcriptome
Provedor de dados: Genetics and Molecular Biology
Autores: Quecini,Vera; Torres,Gisele A.M.; Rosa Jr,Vicente E. de; Gimenes,Marcos A.; Machado,Jeanne B. de M.; Figueira,Antonio V. de O.; Benedito,Vagner; Targon,Maria Luisa P.N.; Cristofani-Yaly,Mariângela.
Plant hormones play a crucial role in integrating endogenous and exogenous signals and in determining developmental responses to form the plant body throughout its life cycle. In citrus species, several economically important processes are controlled by phytohormones, including seed germination, secondary growth, fruit abscission and ripening. Integrative genomics is a powerful tool for linking newly researched organisms, such as tropical woody species, to functional studies already carried out on established model organisms. Based on gene orthology analyses and expression patterns, we searched the Citrus Genome Sequencing Consortium (CitEST) database for Expressed Sequence Tags (EST) consensus sequences sharing similarity to known components of hormone...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Defense responses; Development; Plant growth regulators.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500002

Endophytic bacterial diversity in banana 'Prata Anã' (Musa spp.) roots
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Suzane A.; Xavier,Adelica A.; Costa,Márcia R.; Cardoso,Acleide M.S.; Pereira,Marlon C.T.; Nietsche,Silvia.
The genetic diversity of endophytic bacteria in banana 'Prata Anã' roots was characterized. Two hundred and one endophytic bacteria were isolated, 151 of which were classified as Gram-positive and 50 as Gram-negative. No hypersensitivity response was observed in any of the isolates. The rep-PCR technique generated different molecular profiles for each primer set (REP, ERIC and BOX). Fifty readable loci were obtained and all of the fragments were polymorphic. Amplified ribosomal DNA restriction analysis (ARDRA) of the isolates based on cleavage with four restriction enzymes yielded 45 polymorphic bands and no monomorphic bands. PCR amplified the nifH gene in 24 isolates. 16S rDNA sequencing of the 201 bacterial isolates yielded 102 high-quality sequences....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ARDRA; Bacillus sp.; NifH gene; Rep-PCR; 16S rDNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200016

On Marta Breuer and some of her unpublished drawings of Drosophila spp. male terminalia (Diptera, Drosophilidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Vilela,Carlos Ribeiro; Cunha,Antonio Brito da.
A short illustrated biography of Marta Erps Breuer (1902-1977), designer and lab technician at the University of São Paulo (Universidade de São Paulo), and a former Bauhaus student, is presented. Additionally, twenty fine China ink drawings of the male terminalia of twenty species of Neotropical Drosophila, made by her between October 4th, 1951, and March 24th, 1959, and currently deposited in the archives of the Department of Genetics and Evolutionary Biology of the Institute of Biosciences of the University of São Paulo (Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo), São Paulo, State of São Paulo, Brazil, are included. All illustrated species but one are members of one of the following seven groups of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marta Breuer; André Dreyfus; Aedeagus; Bauhaus; Neotropical region.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300032

Expression of Msx1 and Dlx1 during Dumbo rat head development: correlation with morphological features
Provedor de dados: Genetics and Molecular Biology
Autores: Katerji,Suhair; Vanmuylder,Nathalie; Svoboda,Michal; Rooze,Marcel; Louryan,Stéphane.
The Dumbo rat possesses some characteristics that evoke several human syndromes, such as Treacher-Collins: shortness of the maxillary, zygomatic and mandibular bones, and low position of the ears. Knowing that many homeobox genes are candidates in craniofacial development, we investigated the involvement of the Msx1 and Dlx1 genes in the Dumbo phenotype with the aim of understanding their possible role in abnormal craniofacial morphogenesis and examining the possibility of using Dumbo rat as an experimental model for understanding abnormal craniofacial development. We studied the expression of these genes during craniofacial morphogenesis by RT-PCR method. We used Dumbo embryos at E12 and E14 and included the Wistar strain as a control. Semi-quantitative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dumbo rat; Msx1; Dlx1; Face; Embryo; Development.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200030

Reciprocal regulation between alternative splicing and the DNA damage response
Provedor de dados: Genetics and Molecular Biology
Autores: Botto,Adrian E. Cambindo; Muñoz,Juan C.; Giono,Luciana E.; Nieto-Moreno,Nicolás; Cuenca,Carmen; Kornblihtt,Alberto R.; Muñoz,Manuel J.
Abstract Splicing, the process that catalyzes intron removal and flanking exon ligation, can occur in different ways (alternative splicing) in immature RNAs transcribed from a single gene. In order to adapt to a particular context, cells modulate not only the quantity but also the quality (alternative isoforms) of their transcriptome. Since 95% of the human coding genome is subjected to alternative splicing regulation, it is expected that many cellular pathways are modulated by alternative splicing, as is the case for the DNA damage response. Moreover, recent evidence demonstrates that upon a genotoxic insult, classical DNA damage response kinases such as ATM, ATR and DNA-PK orchestrate the gene expression response therefore modulating alternative splicing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA damage; Alternative splicing; ATR; ATM; DNA-PK.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200310

Morphological patterns of the heteropycnotic chromatin and nucleolar material in meiosis and spermiogenesis of some Pentatomidae (Heteroptera)
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Hederson Vinicius de; Castanhole,Márcia Maria Urbanin; Bicudo,Hermione Elly Melara de Campos; Costa,Luiz Antônio Alves; Itoyama,Mary Massumi.
Pentatomidae is a family of Heteroptera which includes several agriculture pests that have had different aspects of their meiosis and spermiogenesis analyzed. In the present study we analyzed the morphological patterns of the heteropycnotic chromatin and the nucleolar material of Mormidea v-luteum, Oebalus poecilus and Oebalus ypsilongriseus. The three species presented multilobate testes, with three lobes in M. v-luteum and four in the Oebalus species. A karyotype with 2n = 14 chromosomes (12A + XY) was observed in the three species. Several characteristics were common to the three species, such as the absence of a testicular harlequin lobe (a lobe which produces different types of spermatozoa, previously considered a general characteristic of this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nucleolus semi-persistence; Late chromosome migration; Multilobed testes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400014

Genotoxic evaluation of an industrial effluent from an oil refinery using plant and animal bioassays
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Fernando Postalli; Angeli,José Pedro Friedmann; Mantovani,Mário Sérgio; Guedes,Carmen Luisa Barbosa; Jordão,Berenice Quinzani.
Polycyclic aromatic hydrocarbons (PAHs) are genotoxic chemicals commonly found in effluents from oil refineries. Bioassays using plants and cells cultures can be employed for assessing environmental safety and potential genotoxicity. In this study, the genotoxic potential of an oil refinery effluent was analyzed by means of micronucleus (MN) testing of Alium cepa, which revealed no effect after 24 h of treatment. On the other hand, primary lesions in the DNA of rat (Rattus norvegicus) hepatoma cells (HTC) were observed through comet assaying after only 2 h of exposure. On considering the capacity to detect DNA damage of a different nature and of these cells to metabolize xenobiotics, we suggest the association of the two bioassays with these cell types,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; HTC cells; Micronucleus; Comet assay; Petroleum waste disposal.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100028

Fluorescence in situ hybridization (FISH) screening for the 22q11.2 deletion in patients with clinical features of velocardiofacial syndrome but without cardiac anomalies
Provedor de dados: Genetics and Molecular Biology
Autores: Sandrin-Garcia,Paula; Richieri-Costa,Antonio; Tajara,Eloiza Helena; Carvalho-Salles,Andréa Borduchi; Fett-Conte,Agnes Cristina.
The velocardiofacial syndrome (VCFS), a condition associated with 22q11.2 deletions, is characterized by a typical facies, palatal anomalies, learning disabilities, behavioral disturbances and cardiac defects. We investigated the frequency of these chromosomal deletions in 16 individuals with VCFS features who presented no cardiac anomalies, one of the main characteristics of VCFS. Fluorescent in situ hybridization (FISH) with the N25 (D22S75; 22q11.2) probe revealed deletions in ten individuals (62%). Therefore, even in the absence of cardiac anomalies testing for the 22q11.2 microdeletions in individuals showing other clinical features of this syndrome is recommended.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Velocardiofacial syndrome; 22q11.2 deletion.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100006

Network analysis of ABA-dependent and ABA-independent drought responsive genes in Arabidopsis thaliana
Provedor de dados: Genetics and Molecular Biology
Autores: Liu,Shiwei; Lv,Zongyou; Liu,Yihui; Li,Ling; Zhang,Lida.
Abstract Drought is one of the most severe abiotic factors restricting plant growth and yield. Numerous genes functioning in drought response are regulated by abscisic acid (ABA) dependent and independent pathways, but knowledge of interplay between the two pathways is still limited. Here, we integrated transcriptome sequencing and network analyses to explore interplays between ABA-dependent and ABA-independent pathways responding to drought stress in Arabidopsis thaliana. We identified 211 ABA-dependent differentially expressed genes (DEGs) and 1,118 ABA-independent DEGs under drought stress. Functional analysis showed that ABA-dependent DEGs were significantly enriched in expected biological processes in response to water deprivation and ABA stimulus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drought stress; Abscisic acid; RNA-seq; Gene expression; Protein-protein interaction network; Arabidopsis thaliana.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400624

Comparative molecular analysis of Herbaspirillum strains by RAPD, RFLP, and 16S rDNA sequencing
Provedor de dados: Genetics and Molecular Biology
Autores: Soares-Ramos,Juliana R.L.; Ramos,Humberto J.O.; Cruz,Leonardo M.; Chubatsu,Leda S.; Pedrosa,Fábio O.; Rigo,Liu U.; Souza,Emanuel M.
Herbaspirillum spp. are endophytic diazotrophic bacteria associated with important agricultural crops. In this work, we analyzed six strains of H. seropedicae (Z78, M2, ZA69, ZA95, Z152, and Z67) and one strain of H. rubrisubalbicans (M4) by restriction fragment length polymorphism (RFLP) using HindIII or DraI restriction endonucleases, random amplified polymorphic DNA (RAPD), and partial sequencing of 16S rDNA. The results of these analyses ascribed the strains studied to three distinct groups: group I, consisting of M2 and M4; group II, of ZA69; and group III, of ZA95, Z78, Z67, and Z152. RAPD fingerprinting showed a higher variability than the other methods, and each strain had a unique electrophoretic pattern with five of the six primers used....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbaspirillum; RAPD; RFLP; Phylogeny; 16S rDNA.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400019

The PIN1 family gene PvPIN1 is involved in auxin-dependent root emergence and tillering in switchgrass
Provedor de dados: Genetics and Molecular Biology
Autores: Xu,Kaijie; Sun,Fengli; Wang,Yongfeng; Shi,Lili; Liu,Shudong; Xi,Yajun.
Abstract Switchgrass (Panicum virgatum L.; family Poaceae) is a warm-season C4 perennial grass. Tillering plays an important role in determining the morphology of aboveground parts and the final biomass yield of switchgrass. Auxin distribution in plants can affect a variety of important growth and developmental processes, including the regulation of shoot and root branching, plant resistance and biological yield. Auxin transport and gradients in plants are mediated by influx and efflux carriers. PvPIN1, a switchgrass PIN1-like gene that is involved in regulating polar transport, is a putative auxin efflux carrier. Neighbor-joining analysis using sequences deposited in NCBI databases showed that the PvPIN1gene belongs to the PIN1 family and is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Auxin transporter; PvPIN1 gene; TIBA treatment; Tillering; Switchgrass.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100062

De novo transcriptome analysis of Tibetan medicinal plant Dysphania schraderiana
Provedor de dados: Genetics and Molecular Biology
Autores: Fu,Suhong; Lei,Ming; Zhang,Yongqun; Deng,Zhaomin; Shi,Jing; Hao,Doudou.
Abstract Dysphania schraderiana is widely distributed in Lhasa (Tibet, China) and used as a traditional medicine. However, the lack of genetic information hinders the understanding of its physiological processes, such as the biosynthesis of secondary metabolites. Herein, we used Illumina Hiseq4000 platform to sequence the transcriptome of flower and leaf tissues from D. schraderiana for the first time. Totally, 40,142 unigenes were assembled from approximately 5.2 million clean reads. All unigenes underwent gene prediction and were subsequently annotated in a NR (NCBI non-redundant protein) database, COG (Clusters of Orthologous Groups of proteins) database, and KEGG (Kyoto Encyclopedia of Genes and Genomes) database. Among the 40,142 unigenes, 2,579 genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dysphania schraderiana; De novo assembly; Transcriptome; Annotation; Terpenoid biosynthesis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300480

Genetic structure of different cat populations in Europe and South America at a microgeographic level: importance of the choice of an adequate sampling level in the accuracy of population genetics interpretations
Provedor de dados: Genetics and Molecular Biology
Autores: Ruiz-Garcia,Manuel.
The phenotypic markers, coat color, pattern and hair length, of natural domestic cat populations observed in four cities (Barcelona, Catalonia; Palma Majorca, Balearic Islands; Rimini, Italy and Buenos Aires, Argentina) were studied at a microgeographical level. Various population genetics techniques revealed that the degree of genetic differentiation between populations of Felis catus within these cities is relatively low, when compared with that found between populations of other mammals. Two different levels of sampling were used. One was that of "natural" colonies of cat families living together in specific points within the cities, and the other referred to "artificial" subpopulations, or groups of colonies, inhabiting the same district within a city....
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400006

Analysis of the SLC4A1 gene in three Mexican patients with hereditary spherocytosis: report of a novel mutation
Provedor de dados: Genetics and Molecular Biology
Autores: Sánchez-López,Josefina Y.; Camacho-Torres,Ana L.; Ibarra,Bertha; Tintos,Jesús A.; Perea,Francisco J.
We analyzed the SLC4A1 gene in three Mexican patients with Hereditary Spherocytosis (HS). The promoter and all 20 exons were investigated through heteroduplex analysis and DNA sequencing. No DNA changes were detected in one of the three patients. Two well-known polymorphisms, Memphis I and the Diego-a blood group, were detected in another one. In the third, the HS phenotype could be explained by the novel 1885_1888dupCCGG mutation found in heterozygosis. This frameshift mutation is predicted to result in a truncated and unstable protein lacking normal functions.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hereditary spherocytosis; Hemolytic anemia; SLC4A1 gene; AE1 protein; Band 3.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100003

Recombinogenic activity of Pantoprazole® in somatic cells of Drosophila melanogaster
Provedor de dados: Genetics and Molecular Biology
Autores: Lopes,Jeyson Césary; Machado,Nayane Moreira; Saturnino,Rosiane Soares; Nepomuceno,Júlio César.
Pantoprazole® is one of the leading proton pump inhibitors (PPIs) used in the treatment of a variety of diseases related to the upper gastrointestinal tract. However, studies have shown an increased risk of developing gastric cancer, intestinal metaplasia and hyperplasia of endocrine cells with prolonged use. In the present study, the somatic mutation and recombination test (SMART) was employed to determine the mutagenic effects of Pantoprazole on Drosophila melanogaster. Repeated treatments with Pantoprazole were performed on 72-hour larvae of the standard (ST) and high bioactivation (HB) crosses at concentrations of 2.5, 5.0, and 10.0 μM. In addition, doxorubicin (DXR) was administered at 0.4 mM, as a positive control. When administered to ST...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila melanogaster; Mutagenicity; Proton pump inhibitors; Pantoprazole; Recombinogenicity; SMART.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100101

Genetic diversity and population structure of Vriesea reitzii (Bromeliaceae), a species from the Southern Brazilian Highlands
Provedor de dados: Genetics and Molecular Biology
Autores: Soares,Luis Eduardo; Goetze,Márcia; Zanella,Camila M.; Bered,Fernanda.
Abstract The Southern Brazilian Highlands are composed by a mosaic of Mixed Ombrophilous Forest (MOF) and grassland formations, an interesting landscape for the study of population structure. We analyzed the genetic diversity within and among populations of the MOF-endemic bromeliad Vriesea reitzii by genotyping seven nuclear microsatellite loci in 187 individuals from six populations. We characterized levels of genetic diversity and assessed the genetic structure among populations. Vriesea reitzii populations showed high levels of genetic variation (number of alleles 28 - 43, allelic richness 3.589 - 5.531) and moderate levels of genetic differentiation (FST = 0.123, RST = 0.096). The high levels of genetic diversity may be explained by species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mixed Ombrophilous Forest; Bromeliad; Araucaria forest; Gene flow; Conservation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200308

Combined use of mitochondrial and nuclear genetic markers further reveal immature marine turtle hybrids along the South Western Atlantic
Provedor de dados: Genetics and Molecular Biology
Autores: Brito,Cíntia; Vilaça,Sibelle Torres; Lacerda,Ana Luzia; Maggioni,Rodrigo; Marcovaldi,Maria Ângela; Vélez-Rubio,Gabriela; Proietti,Maíra Carneiro.
Abstract Marine turtle hybridization is usually sporadic and involves reports of only a few individuals; however, Brazilian populations have high hybridization rates. Here we investigated the presence of hybrids in morphologically identified immature hawksbills (Eretmochelys imbricata) along the South Western Atlantic (SWA). We sequenced one mitochondrial (D-Loop) and three nuclear DNA (RAG1, RAG2, and CMOS) markers to better understand the patterns and characteristics of hybrids. We identified 22 hybrids (n = 270), 11 of them at the extreme South of the SWA. Uruguay had the highest hybrid frequency in the SWA (~37.5%) followed by southern Brazil with 30%. These are common areas for loggerheads (Caretta caretta) but uncommon for hawksbills, and these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hybridization; Cheloniidae; Genetic markers; Hybrid distribution; Conservation.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400704

Homo sapiens, Homo neanderthalensis and the Denisova specimen: new insights on their evolutionary histories using whole-genome comparisons
Provedor de dados: Genetics and Molecular Biology
Autores: Paixão-Côrtes,Vanessa Rodrigues; Viscardi,Lucas Henrique; Salzano,Francisco Mauro; Hünemeier,Tábita; Bortolini,Maria Cátira.
After a brief review of the most recent findings in the study of human evolution, an extensive comparison of the complete genomes of our nearest relative, the chimpanzee (Pan troglodytes), of extant Homo sapiens, archaic Homo neanderthalensis and the Denisova specimen were made. The focus was on non-synonymous mutations, which consequently had an impact on protein levels and these changes were classified according to degree of effect. A total of 10,447 non-synonymous substitutions were found in which the derived allele is fixed or nearly fixed in humans as compared to chimpanzee. Their most frequent location was on chromosome 21. Their presence was then searched in the two archaic genomes. Mutations in 381 genes would imply radical amino acid changes, with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human evolution; Comparative genomics; Positive selection; Neanderthal; Denisova.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600003

Errata
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400032

Localization of 45S rDNA and telomeric sites on holocentric chromosomes of Rhynchospora tenuis Link (Cyperaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Vanzela,André L.L.; Cuadrado,Angeles; Guerra,Marcelo.
Rhynchospora tenuis Link (Cyperaceae) is a weed widely distributed in Brazil that presents a small number of holocentric chromosomes (2n = 4) with some autopolyploid populations (2n = 8). The haploid number n = 2 is considered as a derivative of the base number x = 5. 45S rDNA probes and telomeric DNA were hybridized in both chromosome races of R. tenuis, looking for indications of chromosome fusions. The results showed that hybridization sites of the telomeric probe were restricted to end chromosome regions whereas rDNA sites were terminally located. The chromosome race with n = 4 exhibited a doubled number of sites, with similar size and location to the hybridized sequences, confirming its autopolyploid origin. Furthermore, the terminal location of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyperaceae; FISH; Holocentric chromosomes; 45S rDNA; Telomere sequences.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200014

Hidden Markov models applied to a subsequence of the Xylella fastidiosa genome
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Cibele Q. da.
Dependencies in DNA sequences are frequently modeled using Markov models. However, Markov chains cannot account for heterogeneity that may be present in different regions of the same DNA sequence. Hidden Markov models are more realistic than Markov models since they allow for the identification of heterogeneous regions of a DNA sequence. In this study we present an application of hidden Markov models to a subsequence of the Xylella fastidiosa DNA data. We found that a three-state model provides a good description for the data considered.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Xylella fastidiosa; Hidden Markov models.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400018

Toxicity and genotoxicity in Astyanax bimaculatus (Characidae) induced by microcystins from a bloom of Microcystis spp
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Ricardo Rocha Pavan da; Pires Junior,Osmindo Rodrigues; Grisolia,Cesar Koppe.
Studies of genotoxicity in fish caused by cyanobacterial microcystins can be useful both in determining the sensitivity of native species, as well as comparing exposure routes. The genotoxicity caused by the microcystins LR and LA from a bloom collected in a eutrophic lake, was revealed in the fish Astyanax bimaculatus, a native species from South America. LC50 (72 h) was determined as 242.81 µg L-1 and LD50 (72 h) as 49.19 µg kg-1 bw. There was a significant increase of DNA damage in peripheral erythrocytes, following intraperitoneal injection (ip) with tested concentrations of 24.58 µg kg-1 bw and 36.88 µg kg-1 bw, as well as through body exposure to a concentration of 103.72 µg L-1. Micronucleus (MN) induction was observed after ip injections of 24.58...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcystin; Comet assay; Fish micronucleus; Necrosis; Apoptosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400023

Parentage test in broad-snouted caimans (Caiman latirostris, Crocodylidae) using microsatellite DNA
Provedor de dados: Genetics and Molecular Biology
Autores: Zucoloto,Rodrigo B.; Verdade,Luciano M.; Villela,Priscilla M.S.; Regitano,Luciana C.A.; Coutinho,Luiz L.
In this study, microsatellite markers, developed for Alligator mississipiensis and Caiman latirostris, were used to assess parentage among individuals from the captive colony of Caiman latirostris at the University of São Paulo, in Piracicaba, São Paulo, Brazil. Many of the females in the colony were full siblings, which made maternal identification difficult due to genotypic similarity. Even so, the most likely mother could be identified unambiguously among offspring in most of the clutches studied. Two non-parental females displayed maternal behavior which would have misled managers in assigning maternity based on behavior alone. This set of variable loci demonstrates the utility of parentage testing in captive propagation programs.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crocodilians; Caiman; Parentage; Microsatellite DNA.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400033

Relationship between the Porcine Stress Syndrome gene and pork quality traits of F2 pigs resulting from divergent crosses
Provedor de dados: Genetics and Molecular Biology
Autores: Band,Guilherme de Oliveira; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Schierholt,Alex Sandro; Silva,Kleibe Moraes; Pires,Aldrin Vieira; Benevenuto Júnior,Augusto Aloísio; Gomide,Lúcio Alberto de Miranda.
The PSS genotypes of 596 F2 pigs produced by initial mating of Brazilian commercial sows and native boars were characterized by PCR-RFLP and the pork quality traits were evaluated. Among the 596 pigs studied, 493 (82.7%) were NN and 103 (17.3%) were Nn. There were no differences between NN and Nn pigs in the following pork qualities: pHu (5.71 ± 0.16 vs 5.70 ± 0.11), intramuscular fat (1.55 ± 0.64% vs 1.65 ± 0.67%), shear force (5552 ± 878 g/1.2 cm vs 5507 ± 826 g/1.2 cm), lightness (44.96 ± 2.05 vs 45.01 ± 1.92), redness (0.64 ± 0.60 vs 0.79 ± 0.55), yellowness (6.62 ± 0.56 vs 6.65 ± 0.48), hue (84.28 ± 5.53 vs 83.41 ± 4.85), or chroma (6.68 ± 0.52 vs 6.73 ± 0.52). However, pork from Nn pigs had a significantly (p &lt; 0.05) lower pH45 (6.41 ± 0.27 vs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meat quality; PCR-RFLP; Pork; PSE; PSS; Pig.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100015

Identification and frequency of transposable elements in Eucalyptus
Provedor de dados: Genetics and Molecular Biology
Autores: Bacci Jr.,Maurício; Soares,Rafael B.S.; Tajara,Eloíza; Ambar,Guilherme; Fischer,Carlos N.; Guilherme,Ivan R.; Costa,Eduardo P.; Miranda,Vitor F.O.
Transposable elements (TE) are major components of eukaryotic genomes and involved in cell regulation and organism evolution. We have analyzed 123,889 expressed sequence tags of the Eucalyptus Genome Project database and found 124 sequences representing 76 TE in 9 groups, of which copia, MuDR and FAR1 groups were the most abundant. The low amount of sequences of TE may reflect the high efficiency of repression of these elements, a process that is called TE silencing. Frequency of groups of TE in Eucalyptus libraries which were prepared with different tissues or physiologic conditions from seedlings or adult plants indicated that developing plants experience the expression of a much wider spectrum of TE groups than that seen in adult plants. These are...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Eucalyptus; Transposable element.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400019

Contribution of DNA repair xeroderma pigmentosum group D genotypes to pancreatic cancer risk in the Chinese Han population
Provedor de dados: Genetics and Molecular Biology
Autores: Yan,Dong; Liang,Xiao-Hui; Ding,Wei; Xu,Xin-Jian; Wang,Xi-Yan.
Abstract This study aimed to determine the association between the polymorphisms and haplotypes in the xeroderma pigmentosum group D (XPD) gene and the risk of pancreatic cancer in the Chinese Han population. SNaPshot was used for genotyping six SNP sites of the XPD gene. Comparisons of the correlations between different genotypes in combination with smoking and the susceptibility to pancreatic cancer were performed. Individual pancreatic cancer risk in patients who carry mutant C alleles (AC, CC, and AC+CC) at rs13181 increased (p &lt; 0.05). Taking non-smoking individuals who carry the AA genotype as a reference, and non-smoking individuals who carry mutant allele C (AC+CC), the risk of pancreatic cancer increased by 3.343 times in individuals who smoked...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pancreatic neoplasm; Human xeroderma pigmentosum group D; Polymorphism; Smoking.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100018

D-loop haplotype diversity in Brazilian horse breeds
Provedor de dados: Genetics and Molecular Biology
Autores: Ianella,Patrícia; Albuquerque,Maria do Socorro Maués; Paiva,Samuel Rezende; Egito,Andréa Alves do; Almeida,Leonardo Daniel; Sereno,Fabiana T. P. S.; Carvalho,Luiz Felipe Ramos; Mariante,Arthur da Silva; McManus,Concepta Margaret.
Abstract The first horses were brought to Brazil by the colonizers after 1534. Over the centuries, these animals evolved and adapted to local environmental conditions usually unsuitable for exotic breeds, thereby originating locally adapted Brazilian breeds. The present work represents the first description of maternal genetic diversity in these horse breeds based on D-loop sequences. A D-Loop HSV-I fragment of 252 bp, from 141 horses belonging to ten Brazilian breeds / genetic groups (locally adapted and specialized breeds) were analysed. Thirty-five different haplotypes belonging to 18 haplogroups were identified with 33 polymorphic sites. Haplotype diversity (varying from 0.20 to 0.96) and nucleotide diversity (varying from 0.0039 to 0.0239) was lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial DNA; Genetic characterization; Equus caballus; Locally adapted breeds; Animal genetic resources.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400604

Sociogenetic structure of Polistes (Aphanilopterus) versicolor Olivier, 1791 colonies (Hymenoptera, Vespidae, Polistini)
Provedor de dados: Genetics and Molecular Biology
Autores: Nagamati Junior,Keize; Simokomaki,Kimie; Gruber,Caroline Vivian; Del Lama,Marco Antonio.
The observation of two distinct, well-defined oviposition areas in nests of the primitively eusocial wasp Polistes versicolor suggests the presence of multiple egg-layers and territorial behaviors. Electrophoretic analysis of enzyme loci in pupae from 35 colonies revealed an average observed heterozygosity of 0.10 and the existence of private polymorphisms, thereby indicating a low dispersion in this species. No evidence of diploid males was found. Phenotypic segregation analysis revealed the presence of more than one egg-laying female in 15 out of 35 colonies, as well as spatially preferential oviposition in 2 out of 13 nests, with distinct oviposition areas. Genetic relatedness estimates for brood were lower than expected for haplodiploid species under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mating system; Relatedness; Multiple egg layers; Territorial behavior; Paper wasps.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400013

Molecular evolution of HoxA13 and the multiple origins of limbless morphologies in amphibians and reptiles
Provedor de dados: Genetics and Molecular Biology
Autores: Singarete,Marina E.; Grizante,Mariana B.; Milograna,Sarah R.; Nery,Mariana F.; Kin,Koryu; Wagner,Günter P.; Kohlsdorf,Tiana.
Developmental processes and their results, morphological characters, are inherited through transmission of genes regulating development. While there is ample evidence that cis-regulatory elements tend to be modular, with sequence segments dedicated to different roles, the situation for proteins is less clear, being particularly complex for transcription factors with multiple functions. Some motifs mediating protein-protein interactions may be exclusive to particular developmental roles, but it is also possible that motifs are mostly shared among different processes. Here we focus on HoxA13, a protein essential for limb development. We asked whether the HoxA13 amino acid sequence evolved similarly in three limbless clades: Gymnophiona, Amphisbaenia and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Development; Evolution; HoxA13; Molecular signatures; Limblessness.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300255

CYP1A1, CYP2E1, GSTM1, GSTT1, GSTP1, and TP53 polymorphisms: do they indicate susceptibility to chronic obstructive pulmonary disease and non-small-cell lung cancer?
Provedor de dados: Genetics and Molecular Biology
Autores: Gaspar,Pedro; Moreira,José; Kvitko,Katia; Torres,Martiela; Moreira,Ana; Weimer,Tania.
Gene polymorphisms of phase I (CYP1A1 and CYP2E of cytochrome P,) and phase II (GSTM1, GSTT1 and GSTP1 of glutathione-S-transferase,) enzymes and the TP53 tumor suppressor gene were studied as markers in a sample of 262 Brazilians of European descent, the sample consisting of 97 patients with non-small-cell lung cancer (NSCLC), 75 patients with chronic obstructive pulmonary disease (COPD) and 90 control individuals. For NSCLC, we found no significant relationship between any of the markers studied and susceptibility to this disease. With respect to COPD, although the distribution of the CYP1A1, GSTM1, GSTP1, GSTT1 and TP53 genotypes was similar to that of the controls the frequency of the CYP2E1*1A/*5B heterozygote was about 6 times higher in COPD patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CYPs; GSTs and TP53 polymorphisms; Chronic obstructive pulmonary disease; Non-small-cell lung cancer; Genetic susceptibility.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200001

Association of FOSL1 copy number alteration and triple negative breast tumors
Provedor de dados: Genetics and Molecular Biology
Autores: Serino,Leandro Tamião Rodrigues; Jucoski,Tayana Schultz; Morais,Stephanie Bath de; Fernandes,Cíntia Callegari Coêlho; Lima,Rubens Silveira de; Urban,Cícero Andrade; Cavalli,Luciane Regina; Cavalli,Iglenir João; Ribeiro,Enilze Maria de Souza Fonseca.
Abstract Copy number alterations (CNAs) are a frequent feature in human breast cancer, and one of the hallmarks of genomic instability. The FOSL1, GSTP1 and CCND1 genes are located at 11q13, a cytoband commonly affected by CNA in breast cancer, with relevant function in progression and invasion. Our main goal was to analyze CNAs of these genes and determine their association with breast cancer subtypes. Seventy-three cases of invasive breast tumors [52 Luminal, 7 HER2+ and 14 triple negative (TNBC) subtypes] were analyzed by TaqMan assays. CNAs were observed for all genes, with gains more frequently observed. Gains of the FOSL1 gene were observed in 71% of the cases. This gene was the only one with a statistically significant difference (p&lt;0.001) among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triple negative breast cancer; TNBC; DNA copy number alterations; CNA.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100026

Rapid screening for aluminum tolerance in maize (Zea mays L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Giaveno,Carlos Daniel; Miranda Filho,José B.
A significant decrease in maize grain yield due to aluminum toxicity is considered to be one of the most important agricultural problems for tropical regions. Genetic improvement is a useful approach to increase maize yield in acid soils, but this requires a rapid and reliable method to discriminate between genotypes. In our work we investigated the feasibility of using hematoxylin staining (HS) to detect Al-tolerant plants at the seedling stage. The original population along with two populations obtained after one cycle of divergent selection were evaluated by net root growth (NRG) and HS after 7 days in nutrient solution. Results showed a negative correlation between NRG and HS in all populations, in which sensitive plants, characterized by low NRG,...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400024

Cytogenetic analysis of three catfish species of the family Pseudopimelodidae (Teleostei, Siluriformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Martinez,Emanuel Ricardo Monteiro; Nirchio,Mauro; Granado,Angel; Foresti,Fausto; Oliveira,Claudio.
Cytogenetic analyses performed in Cephalosilurus apurensis, Microglanis aff. cottoides and Pseudopimelodus bufonius revealed that the three species have 2n = 54 chromosomes: C. apurensis presented six metacentric (M), 28 submetacentric (SM), 14 subtelolocentric (ST), and six acrocentric (A) chromosomes, while M. aff. cottoides showed 10M, 32SM, 10ST and 2A, and P. bufonius had 12M, 30SM and 12ST. The nucleolus organizer regions (NORs) were present on the short arm of a middle-sized ST pair, identified as pair 19, in C. apurensis NORs were found on the short arm of a middle-sized ST (pair 23) and on the long arm of a middle-sized ST (pair 22) in M. aff. cotttoides and on the short arm of three middle-sized ST pairs, identified as pairs 9, 10 and 11, in P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Chromosomes; C-banding; Ag-NOR; Fish.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400015

Incorporating different proportions of exotic maize germplasm into two adapted populations
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Manoel Xavier dos; Pollak,Linda Maria; Pacheco,Cleso Antônio Patto; Guimarães,Paulo Evaristo Oliveira; Peternelli,Luiz Alexandre; Parentoni,Sidney Netto; Nass,Luciano Lourenço.
Maize breeders frequently wish to use exotic germplasm in their breeding programs without losing specific characteristics of their adapted material. The objective of this study was to determine the optimal proportions of exotic germplasm to incorporate into adapted populations (F2 = 50% exotic, BC1 = 25% exotic, BC2 = 12.5% exotic and BC3 = 6.25% exotic) to form the initial foundation population and to determine the heterosis between adapted x exotics. We used six exotic populations of different origins and two adapted populations representing a Brazilian heterotic pattern. In 1993-94 and 1994-95, the parents, F1, F2, BC1, BC2, BC3 and four checks were evaluated in six environments in central Brazil using an 8 x 9 simple rectangular lattice design. Higher...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200033

Mitochondrial DNA polymorphism and heteroplasmy in populations of the three species of Tropidurus of the nanuzae group (Squamata, Tropiduridae)
Provedor de dados: Genetics and Molecular Biology
Autores: Passoni,José Carlos; Benozzati,Maria Lúcia; Rodrigues,Miguel Tréfaut.
The nanuzae group of lizards includes three species, Tropidurus nanuzae, T. divaricatus and T. amathites. The first species is found along Serra do Espinhaço, in eastern Brazil, and the other two in the northern region of the Brazilian State of Bahia, in continental dunes on both margins of the São Francisco River. Restriction fragment length polymorphisms (RFLP) of the mtDNA in these species were detected in 53 restriction sites. Site and fragment length polymorphisms were characterized, and cases of heteroplasmy involving length variation were observed. In T. divaricatus, these variations involved changes of 50-200 bp, probably in the control region of the molecule. In T. amathites, variation was apparently due to duplication/deletion of a 400-bp...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200019

Detection of diploid males in a natural colony of the cleptobiotic bee Lestrimelitta sp (Hymenoptera, Apidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Tavares,Mara Garcia; Carvalho,Carlos Roberto; Soares,Fernanda Aparecida Ferrari; Fernandes,Anderson.
When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to be diploid, since, through cytometric analysis, it was demonstrated that the mean genome size of both males and females was the same (C = 0.463 pg), and, furthermore, cytogenetic analysis demonstrated that both had 2n = 28 chromosomes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetic; Flow cytometry; Genome size; Karyotype; Stingless bee.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300019

Identification of a novel COL1A1 frameshift mutation, c.700delG, in a Chinese osteogenesis imperfecta family
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,Xiran; Pei,Yu; Dou,Jingtao; Lu,Juming; Li,Jian; Lv,Zhaohui.
Osteogenesis imperfecta (OI) is a family of genetic disorders associated with bone loss and fragility. Mutations associated with OI have been found in genes encoding the type I collagen chains. People with OI type I often produce insufficient α1-chain type I collagen because of frameshift, nonsense, or splice site mutations in COL1A1 or COL1A2. This report is of a Chinese daughter and mother who had both experienced two bone fractures. Because skeletal fragility is predominantly inherited, we focused on identifying mutations in COL1A1 and COL1A2 genes. A novel mutation in COL1A1, c.700delG, was detected by genomic DNA sequencing in the mother and daughter, but not in their relatives. The identification of this mutation led to the conclusion that they were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteogenesis imperfecta; Chinese OI type 1 family; Type I collagen; Sequence analysis; Frameshift mutation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100001

Karyotypic conservatism in samples of Characidium cf. zebra (Teleostei, Characiformes, Crenuchidae): physical mapping of ribosomal genes and natural triploidy
Provedor de dados: Genetics and Molecular Biology
Autores: Pansonato-Alves,José Carlos; Oliveira,Claudio; Foresti,Fausto.
Basic and molecular cytogenetic analyses were performed in specimens of Characidium cf. zebra from five collection sites located throughout the Tietê, Paranapanema and Paraguay river basins. The diploid number in specimens from all samples was 2n = 50 with a karyotype composed of 32 metacentric and 18 submetacentric chromosomes in both males and females. Constitutive heterochromatin was present at the centromeric regions of all chromosomes and pair 23, had additional interstitial heterochromatic blocks on its long arms. The nucleolar organizer regions (NORs) were located on the long arms of pair 23, while the 5S rDNA sites were detected in different chromosomes among the studied samples. One specimen from the Alambari river was a natural triploid and had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characidium; Heterochromatin; 18S rDNA; 5S rDNA; Natural triploidy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200007

Oxidative stress response in sugarcane
Provedor de dados: Genetics and Molecular Biology
Autores: Soares Netto,Luis Eduardo.
Oxidative stress response in plants is still poorly understood in comparison with the correspondent phenomenon in bacteria, yeast and mammals. For instance, nitric oxide is assumed to play various roles in plants although no nitric oxide synthase gene has yet been isolated. This research reports the results of a search of the sugarcane expressed sequence tag (SUCEST) database for homologous sequences involved in the oxidative stress response. I have not found any gene similar to nitric oxide synthase in the SUCEST database although an alternative pathway for nitric oxide synthesis was proposed. I have also found several genes involved in antioxidant defense, e.g. metal chelators, low molecular weight compounds, antioxidant enzymes and repair systems....
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100014

Genetic variation for growth and selection in adult plants of Eucalyptus badjensis
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Paulo Eduardo Telles dos; Paludzyszyn Filho,Estefano; Silva,Lorenzo Teixeira de Melo da; Vandresen,Paula Burigo.
Abstract The aim of this study was to evaluate Eucalyptus badjensis concerning the genetic variation for growth traits and the potential of the species in supporting a breeding programme. The field trial was a provenance/progeny test established in Campina da Alegria, Santa Catarina, Brazil (latitude 26°52′05.1″ S, longitude 51°48′47.5″ W, altitude 1,015 m) in a soil classified as Latossolic Alumino-Ferric Brown Nitosol. The experiment comprised 60 open-pollinated progenies from the provenances Glenbog and Badja State Forest, New South Wales, Australia. Ten replicates and plots with six plants in row were used. At the age of 17 years, 279 trees were assessed for diameter of the bole at breast height (DBH), total tree height (H) and volume of wood with bark...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalypt; Progeny test; Genetic parameters; Breeding; Genetic gain.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400457

Adiponectin promoter polymorphisms are predictors of lipid profile improvement after bariatric surgery
Provedor de dados: Genetics and Molecular Biology
Autores: Gasparotto,Aline Simas; Borges,Diego Olschowsky; Zandoná,Marília Remuzzi; Ramos,Mauricio Jacques; Meihnardt,Nelson Guardiola; Mattevi,Vanessa S.
Abstract Our aim was to investigate if single nucleotide polymorphisms (SNPs) located in the 5′ regions of leptin (LEP, -2548 G &gt; A, rs7799039), resistin (RETN, -420 C &gt; G, rs1862513) and adiponectin (ADIPOQ, -11391 G &gt; A, rs17300539 and -11377 C &gt; G, rs266729) genes were related to changes in body mass index (BMI) and metabolic variables after bariatric surgery in 60 extremely obese individuals. At baseline, ADIPOQ -11391 A-allele carriers showed higher plasma adiponectin and lower total cholesterol levels when compared to G/G homozygotes. Approximately 32 months post-surgery, a mean reduction of 35% in BMI and an important improvement in metabolic profiles were observed. In addition, for the ADIPOQ -11377 polymorphism, a higher decrease in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adiponectin; Polymorphism; Obesity; Lipid profile; Bariatric surgery.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500736

Genetic diversity and population structure of bocachico Prochilodus magdalenae (Pisces, Prochilodontidae) in the Magdalena River basin and its tributaries, Colombia
Provedor de dados: Genetics and Molecular Biology
Autores: Berdugo,Gilberto Orozco; Narváez Barandica,Juan C.
Prochilodus magdalenae is an endemic freshwater fish that occurs in the Magdalena, Sinú and Atrato hydrographic basins. It has an important economic role and is a food resource for the artisanal fishing communities. Its socioeconomic importance contrasts with the current status of its fisheries, where stocks are being depleted. Considering its importance and lack of information on its genetic structure, we used seven microsatellite markers to assess the genetic structure of wild populations of P. magdalenae. The genetic diversity was assessed and the population genetic structure was estimated through Fst, analysis of molecular variance and Bayesian analysis. A total of 290 alleles were found in all loci throughout all population. The high polymorphism...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neotropical fish; Microsatellite; Conservation genetics; Population genetics.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100008

Evolution of flatworm central nervous systems: Insights from polyclads
Provedor de dados: Genetics and Molecular Biology
Autores: Quiroga,Sigmer Y.; Carolina Bonilla,E.; Marcela Bolaños,D.; Carbayo,Fernando; Litvaitis,Marian K.; Brown,Federico D.
The nervous systems of flatworms have diversified extensively as a consequence of the broad range of adaptations in the group. Here we examined the central nervous system (CNS) of 12 species of polyclad flatworms belonging to 11 different families by morphological and histological studies. These comparisons revealed that the overall organization and architecture of polyclad central nervous systems can be classified into three categories (I, II, and III) based on the presence of globuli cell masses -ganglion cells of granular appearance-, the cross-sectional shape of the main nerve cords, and the tissue type surrounding the nerve cords. In addition, four different cell types were identified in polyclad brains based on location and size. We also characterize...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain; FMRFamide; Globuli cell masses; Orthogon; Serotonin.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300233

Investigations on DNA damage and frequency of micronuclei in occupational exposure to electromagnetic fields (EMFs) emitted from video display terminals (VDTs)
Provedor de dados: Genetics and Molecular Biology
Autores: Lakshmi,NK; Tiwari,R; Bhargava,SC; Ahuja,YR.
The potential effect of electromagnetic fields (EMFs) emitted from video display terminals (VDTs) to elicit biological response is a major concern for the public. The software professionals are subjected to cumulative EMFs in their occupational environments. This study was undertaken to evaluate DNA damage and incidences of micronuclei in such professionals. To the best of our knowledge, the present study is the first attempt to carry out cytogenetic investigations on assessing bioeffects in personal computer users. The study subjects (n = 138) included software professionals using VDTs for more than 2 years with age, gender, socioeconomic status matched controls (n = 151). DNA damage and frequency of micronuclei were evaluated using alkaline comet assay...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electromagnetic fields (EMFs); Video display terminals (VDTs); Comet assay; DNA damage; Micronuclei.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100026

Heterochromatin heterogeneity and chromosome heteromorphism in Cerdocyon thous (Mammalia, Canidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Hatanaka,Terumi; Tambasco,Antonio Junqueira; Galetti Junior,Pedro Manoel.
Few cytogenetic studies have been conducted on South American canids. Cerdocyon thous is a representative of the Canidae, living in the forests, open wooded areas and savannahs of South America. Compared to other canid species, C. thous has a large proportion of metacentric and submetacentric chromosomes as well as a large amount of constitutive heterochromatin, especially along the short arm of submetacentric chromosomes. In the present study, different chromosome banding methods were used to characterize the heterogeneous nature of the large heterochromatic segments and to propose an organizational model for this segment that occupies the entire short arm of most two-armed chromosomes. Furthermore, chromosome heteromorphism related to the short arm of...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200009

Identification and phylogenetic inferences on stocks of sharks affected by the fishing industry off the Northern coast of Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues-Filho,Luis Fernando da Silva; Rocha,Tainá Carreira da; Rêgo,Péricles Sena do; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
The ongoing decline in abundance and diversity of shark stocks, primarily due to uncontrolled fishery exploitation, is a worldwide problem. An additional problem for the development of conservation and management programmes is the identification of species diversity within a given area, given the morphological similarities among shark species, and the typical disembarkation of processed carcasses which are almost impossible to differentiate. The main aim of the present study was to identify those shark species being exploited off northern Brazil, by using the 12S-16S molecular marker. For this, DNA sequences were obtained from 122 specimens collected on the docks and the fish market in Bragança, in the Brazilian state of Pará. We identified at least 11...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sharks; Molecular identification; Phylogenetic; MtDNA; Conservation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200031

Supernumerary chromosomes in the pufferfish Sphoeroides spengleri: first occurrence in marine Teleostean Tetraodontiformes fish
Provedor de dados: Genetics and Molecular Biology
Autores: Alves,Anderson Luis; Porto-Foresti,Fábio; Oliveira,Claudio; Foresti,Fausto.
Cytogenetic analyses carried out in eight specimens of Sphoeroides spengleri revealed the presence of 2n = 46 chromosomes (20 M/SM and 26 ST/A). Besides the standard karyotypical set, the presence of B microchromosomes was observed in two individuals, ranging from 0 to 2 microchromosomes per cell. A karyotype composed by 2n = 46 chromosomes with occurrence of M and SM chromosomes is considered basal for the species from the clade comprising the families Tetraodontidae, Balistidae, and Diodontidae, although it represents a derived condition for the order Tetraodontiformes, whose basal karyotype would be composed by 2n = 48 acrocentric chromosomes. The occurrence of B microchromosomes in marine Tetraodontiformes fish was not known, and this represents the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B chromosome; Pufferfish; Sphoeroides spengleri.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200014

pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops
Provedor de dados: Genetics and Molecular Biology
Autores: Guidelli,Giovanna V.; Mattiello,Lucia; Gallinari,Rafael H.; Lucca,Paulo Cezar de; Menossi,Marcelo.
Abstract The successful development of genetically engineered monocots using Agrobacterium-mediated transformation has created an increasing demand for compatible vectors. We have developed a new expression vector, pGVG, for efficient transformation and expression of different constructs for gene overexpression and silencing in sugarcane. The pCAMBIA2300 binary vector was modified by adding Gateway recombination sites for fast gene transfer between vectors and the maize polyubiquitin promoter Ubi-1 (ZmUbi1), which is known to drive high gene expression levels in monocots. Transformation efficiency using the pGVG vector reached up to 14 transgenic events per gram of transformed callus. Transgenic plants expressing the β-glucuronidase (GUS) reporter gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monocots; Sugarcane; Vector; Gateway technology; Genetic transformation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300450

Alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma U343 cell line
Provedor de dados: Genetics and Molecular Biology
Autores: Carminati,Patricia Oliveira; Mello,Stephano Spano; Fachin,Ana Lucia; Junta,Cristina Moraes; Sandrin-Garcia,Paula; Carlotti,Carlos Gilberto; Donadi,Eduardo Antonio; Passos,Geraldo Aleixo Silva; Sakamoto-Hojo,Elza Tiemi.
Gliomas are the most common tumors in the central nervous system, the average survival time of patients with glioblastoma multiforme being about 1 year from diagnosis, in spite of harsh therapy. Aiming to study the transcriptional profiles displayed by glioma cells undergoing cisplatin treatment, gene expression analysis was performed by the cDNA microarray method. Cell survival and apoptosis induction following treatment were also evaluated. Drug concentrations of 12.5 to 300 μM caused a pronounced reduction in cell survival rates five days after treatment, whereas concentrations higher than 25 μM were effective in reducing the survival rates to ~1%. However, the maximum apoptosis frequency was 20.4% for 25 μM cisplatin in cells analyzed at 72 h,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Cisplatin; Gene expression; Glioma.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100027

Nuclear DNA content in 20 species of Siluriformes (Teleostei: Ostariophysi) from the Neotropical region
Provedor de dados: Genetics and Molecular Biology
Autores: Fenerich,Paulo César; Foresti,Fausto; Oliveira,Claudio.
In the present study, 20 species of Siluriformes fish were analyzed in order to determine their nuclear DNA content and compare these data with their diploid number. In addition, the extension and importance of the changes that occurred during the process of diversification in the group of Neotropical freshwater catfish were investigated. The only species studied of the family Doradidae, Rhinodoras d'orbignyi (2n = 58), presented 3.46 ± 0.13 pg of DNA. Among the species of the family Heptapteridae, the values of nuclear DNA content and the diploid numbers ranged from 1.13 ± 0.09 pg of DNA in Pimelodella sp. (2n = 46) to 2.38 ± 0.07 pg of DNA in Imparfinis mirini (2n = 58). The family Loricariidae showed the widest variation in diploid number and nuclear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nuclear DNA; DNA content; Chromosome; Fish; Evolution.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300008

Mapping of ribosomal genes and chromosomal markers in three species of the genus  Serrasalmus  (Characidae, Serrasalminae) from the Amazon basin
Provedor de dados: Genetics and Molecular Biology
Autores: Nakayama,Celeste M.; Feldberg,Eliana; Bertollo,Luiz Antonio C.
Karyotypic characteristics of three species of the genus  serrasalmus  ( S. altispinnis ,  S. gouldingi  and  S. Serrulatus ) from the middle and lower Negro River, Amazon Basin, were investigated using different staining techniques and Fluorescent  in situ  hybridization with 5S and 18S rDNA probes. The diploid number was invariably 2n = 60 and the fundamental number was FN = 110. Nevertheless, the karyotypes differed from each other in composition: 24m, 20sm, 6st, 10a in  S. altispinnis ; 22m, 22sm, 6st, 10a in  S. gouldingi  and 20m, 22sm, 8st, 10a in  S. serrulatus.  The karyotype of  S. altispinnis  differed from the one previously described in a population from the Pitinga River. C-positive constitutive heterochromatin was mainly pericentromeric in...
Palavras-chave: Chromosome banding; Cytotaxonomy of piranhas; FISH; Karyotype; Ribosomal DNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500011

An approach for understanding the heredity of two quality traits (head color and tightness) in globe artichoke (Cynara scolymus L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Cravero,Vanina Pamela; Picardi,Liliana Amelia; Cointry,Enrique Luis.
The inheritance of head color and tightness in globe artichoke was studied utilizing crosses between inbreed lines and between clones and self-pollinated clones from different genetic origins. These genetic materials were sowed in a completely randomized design with 20 plants per plot and genotype. Globe artichoke heads were classified into three colors (purple-green, purple and green) and three head tightness types (compact, fairly compact and soft) and the segregating ratios for these traits tested in each offspring using the chi-square test. Crosses between green and purple inbreed lines produced only purple-green heads but F2 generation segregated at a purple-green:purple:green ratio of 9:3:4. The self-pollinated compact head clones produced a compact...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Globe artichoke; Heredity; Inbreed lines; Clones; Epistasis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300016

Evaluation of genotoxicity using the micronucleus assay and nuclear abnormalities in the tropical sea fish Bathygobius soporator (Valenciennes, 1837) (Teleostei, Gobiidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Galindo,Toni P.; Moreira,Lília M.
The micronucleus and nuclear abnormalities assays have been used increasingly to evaluate genotoxicity of many compounds in polluted aquatic ecossystems. The aim of this study is to verify the efficiency of the micronucleus assay and nuclear abnormality assay in field and laboratory work, when using erythrocytes of the tropical marine fish Bathygobius soporator as genotoxicity biomarkers. Gill peripheral blood samples were obtained from specimens of Bathygobius soporator. In order to investigate the frequencies of micronuclei and to assess the sensitivity of species, the results were compared with samples taken at the reference site and maintained in the laboratory, and fish treated with cyclophosphamide. The micronucleus assay was efficient in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronucleus assay; Nuclear abnormality assay; Genotoxicity; Frillfin goby; Bathygobius soporator.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200029

Biometric variability of goat populations revealed by means of principal component analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Pires,Luanna Chácara; Machado,Théa M. Medeiros; Araújo,Adriana Mello; Olson,Timothy A.; Silva,João Batista Lopes da; Torres,Robledo Almeida; Costa,Márcio da Silva.
The aim was to analyze variation in 12 Brazilian and Moroccan goat populations, and, through principal component analysis (PCA), check the importance of body measures and their indices as a means of distinguishing among individuals and populations. The biometric measurements were wither height (WH), brisket height (BH) and ear length (EL). Thorax depth (WH-BH) and the three indices, TD/WH, EL/TD and EL/WH, were also calculated. Of the seven components extracted, the first three principal components were sufficient to explain 99.5% of the total variance of the data. Graphical dispersion by genetic groups revealed that European dairy breeds clustered together. The Moroccan breeds were separated into two groups, one comprising the Drâa and the other the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biometrics; Genetic resources; Goat breeds; Morphometrics; Population characterization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500011

Polymorphisms of arylamine N-acetyltransferase2 and risk of lung and colorectal cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Mahasneh,Amjad; Jubaili,Amal; El Bateiha,Ahmed; Al-Ghazo,Mohammad; Matalka,Ismail; Malkawi,Mousa.
The arylamine N-acetyltransferase 2 (NAT2) enzymes detoxify a wide range of naturally occurring xenobiotics including carcinogens and drugs. Point mutations in the NAT2 gene result in the variant alleles M1 (NAT2 *5A), M2 (NAT2*6A), M3 (NAT2*7) and M4 (NAT2 *14A) from the wild-type WT (NAT2 *4) allele. The current study was aimed at screening genetic polymorphisms of NAT2 gene in 49 lung cancer patients, 54 colorectal cancer patients and 99 cancer-free controls, using PCR-RFLP. There were significant differences in allele frequencies between lung cancer patients and controls in the WT, M2 and M3 alleles (p &lt; 0.05). However, only M2 and M3 allele frequencies were different between colorectal cancer patients and controls (p &lt; 0.05). There was a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NAT2; Lung cancer; Colorectal cancer; Polymorphisms.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500003

Multigene families encode the major enzymes of antioxidant metabolism in Eucalyptus grandis L
Provedor de dados: Genetics and Molecular Biology
Autores: Teixeira,Felipe Karam; Menezes-Benavente,Larissa; Galvão,Vinícius Costa; Margis-Pinheiro,Márcia.
Antioxidant metabolism protects cells from oxidative damage caused by reactive oxygen species (ROS). In plants, several enzymes act jointly to maintain redox homeostasis. Moreover, isoform diversity contributes to the fine tuning necessary for plant responses to both exogenous and endogenous signals influencing antioxidant metabolism. This study aimed to provide a comprehensive view of the major classes of antioxidant enzymes in the woody species Eucalyptus grandis. A careful survey of the FORESTs data bank revealed 36 clusters as encoding antioxidant enzymes: six clusters encoding ascorbate peroxidase (APx) isozymes, three catalase (CAT) proteins, three dehydroascorbate reductase (DHAR), two glutathione reductase (GR) isozymes, four monodehydroascorbate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus; Antioxidant metabolism; Catalase; Ascorbate-gluthatione cycle.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400007

Patterns of heterochromatin distribution in plant chromosomes
Provedor de dados: Genetics and Molecular Biology
Autores: Guerra,Marcelo.
The C-band distribution patterns of 105 angiosperm species were compared to identify general patterns or preferential sites for heterochromatin. The base-specific fluorochrome reaction of heterochromatin for 58 of these species and the role played by the average chromosome size in band distribution were also considered. The results showed that heterochromatin was preferentially located in similar chromosome regions, regardless of the distance from the centromere. This trend results in generalized bands, with heterochromatin distribution being identical in most chromosomes of a karyotype. Such bands very often displayed the same fluorochrome reaction, suggesting possible repeat transfer between non-homologous sites. Chromosome size may also play a role in...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400049

Comparative analysis of microsatellite variability in five macaw species (Psittaciformes, Psittacidae): application for conservation
Provedor de dados: Genetics and Molecular Biology
Autores: Presti,Flavia T.; Oliveira-Marques,Adriana R.; Caparroz,Renato; Biondo,Cibele; Miyaki,Cristina Y.
Cross-amplification was tested and variability in microsatellite primers (designed for Neotropical parrots) compared, in five macaw species, viz., three endangered blue macaws (Cyanopsitta spixii [extinct in the wild], Anodorhynchus leari [endangered] and Anodorhynchus hyacinthinus [vulnerable]), and two unthreatened red macaws (Ara chloropterus and Ara macao). Among the primers tested, 84.6% successfully amplified products in C. spixii, 83.3% in A. leari, 76.4% in A. hyacinthinus, 78.6% in A. chloropterus and 71.4% in A. macao. The mean expected heterozygosity estimated for each species, and based on loci analyzed in all the five, ranged from 0.33 (A. hyacinthinus) to 0.85 (A. macao). As expected, the results revealed lower levels of genetic variability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Cross-amplification; Macaw; Microsatellite; Psittaciformes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200030

Micronuclei induction in Rana catesbeiana tadpoles by the pyrethroid insecticide lambda-cyhalothrin
Provedor de dados: Genetics and Molecular Biology
Autores: Campana,Marcela Alejandra; Panzeri,Ana María; Moreno,Víctor Jorge; Dulout,Fernando Noel.
Pyrethroid lambda-cyhalothrin genotoxicity was evaluated using the micronucleus test in Rana catesbeiana tadpoles. The effects of concentration and exposure time on the micronuclei frequency were studied in blood smears obtained from tadpoles exposed to four concentrations (0.02, 0.1, 0.2 and 0.4 mg/L) of the compound for 24, 48, 72 and 96 h and 8, 15, 20 and 30 days. As a positive control, tadpoles were exposed to cyclophosphamide (5 mg/L). The micronucleated cell frequency was expressed per 1,000 cells. R. catesbeiana tadpoles exposed to increasing concentrations of lambda-cyhalothrin showed an increase in the micronuclei frequency in peripheral blood. Tadpoles exposed to cyclophosphamide (CP) also showed a significant increase in micronucleated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Micronucleus test; Lambda-cyhalothrin; Tadpoles.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100016

A putative resistant DNA marker for wool yellowing susceptibility in sheep
Provedor de dados: Genetics and Molecular Biology
Autores: Benavides,M.V.; Damak,S.; Maher,A.P.
An Australian Merino flock was screened for low (resistant) and high (susceptible) yellow predictive colour (YPC) breeding values in order to compare extreme individuals using the differential display of mRNA technique. One differentially expressed cDNA band was visualised only in the resistant group. This band showed no identity with the DNA sequences of public databases; however, they showed short homologies with three database sequences related to transmembrane signalling functions. The use of these candidate genes as DNA markers needs to be confirmed against sheep with a wide range of susceptibility to wool yellowing to verify the results.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200017

Molecular cloning and characterization of a novel freezing-inducible DREB1/CBF transcription factor gene in boreal plant Iceland poppy (Papaver nudicaule)
Provedor de dados: Genetics and Molecular Biology
Autores: Huang,Zhuo; He,Jiao; Zhong,Xiao-Juan; Guo,Han-Du; Jin,Si-Han; Li,Xi; Sun,Ling-Xia.
Abstract DREB1 of the AP2/ERF superfamily plays a key role in the regulation of plant response to low temperatures. In this study, a novel DREB1/CBF transcription factor, PnDREB1, was isolated from Iceland poppy (Papaver nudicaule), a plant adaptive to low temperature environments. It is homologous to the known DREB1s of Arabidopsis and other plant species. It also shares similar 3D structure, and conserved and functionally important motifs with DREB1s of Arabidopsis. The phylogenetic analysis indicated that the AP2 domain of PnDREB1 is similar to those of Glycine max, Medicago truncatula, and M. sativa. PnDREB1 is constitutively expressed in diverse tissues and is increased in roots. qPCR analyses indicated that PnDREB1 is significantly induced by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DREB1; Expression profile; Freezing stress; Iceland poppy; Transcription factor.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400616

Genetic variation and evolutionary demography of Fenneropenaeus chinensis populations, as revealed by the analysis of mitochondrial control region sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Kong,Xiao Yu; Li,Yu Long; Shi,Wei; Kong,Jie.
Genetic variation and evolutionary demography of the shrimp Fenneropenaeus chinensis were investigated using sequence data of the complete mitochondrial control region (CR). Fragments of 993 bp of the CR were sequenced for 93 individuals from five localities over most of the species' range in the Yellow Sea and the Bohai Sea. There were 84 variable sites defining 68 haplotypes. Haplotype diversity levels were very high (0.95 ± 0.03-0.99 ± 0.02) in F. chinensis populations, whereas those of nucleotide diversity were moderate to low (0.66 ± 0.36%-0.84 ± 0.46%). Analysis of molecular variance and conventional population statistics (F ST) revealed no significant genetic structure throughout the range of F. chinensis. Mismatch distribution, estimates of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fenneropenaeus chinensis; MtDNA; Isolation with migration (IM) coalescence; Historical demography; Population expansion.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200029

Use of direct and iterative solvers for estimation of SNP effects in genome-wide selection
Provedor de dados: Genetics and Molecular Biology
Autores: Pimentel,Eduardo da Cruz Gouveia; Sargolzaei,Mehdi; Simianer,Henner; Schenkel,Flávio Schramm; Liu,Zengting; Fries,Luiz Alberto; Queiroz,Sandra Aidar de.
The aim of this study was to compare iterative and direct solvers for estimation of marker effects in genomic selection. One iterative and two direct methods were used: Gauss-Seidel with Residual Update, Cholesky Decomposition and Gentleman-Givens rotations. For resembling different scenarios with respect to number of markers and of genotyped animals, a simulated data set divided into 25 subsets was used. Number of markers ranged from 1,200 to 5,925 and number of animals ranged from 1,200 to 5,865. Methods were also applied to real data comprising 3081 individuals genotyped for 45181 SNPs. Results from simulated data showed that the iterative solver was substantially faster than direct methods for larger numbers of markers. Use of a direct solver may allow...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breeding value; Genomic selection; Mixed model equations; Numerical method.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100033

H19DMR methylation analysis in patients with Beckwith-Wiedemann syndrome and isolated hemihyperplasia
Provedor de dados: Genetics and Molecular Biology
Autores: Gomes,Marcus Vinícius de Matos; Santos,Sílvio Avelino dos; Ramos,Ester Silveira.
Beckwith-Wiedemann syndrome (BWS) is a congenital overgrowth disorder of complex and heterogeneous etiology involving alterations in genomic imprinting. The cause of isolated hemihyperplasia (IHH) is unknown but might be due to partial or incomplete expression of BWS because both these conditions share predisposition for the same types of neoplasias. We investigated the methylation pattern of the putative imprinting control region H19DMR using peripheral blood from 12 patients, six with clinical features of BWS and six with IHH. All the patients had normal karyotypes and paternal uniparental disomy (UPD) was excluded in 10 informative cases. The normal H19DMR methylation pattern was found in eight informative patients, indicating that H19DMR methylation...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Beckwith-Wiedemann syndrome; Isolated hemihyperplasia; Genomic imprinting; DNA methylation; Uniparental disomy; H19DMR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200005

Allozyme differentiation of two populations of the genus Neoplecostomus Eigenmann &amp;amp; Eigenmann, 1888 (Teleostei, Loricariidae) from the upper Paraná River basin, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Reusing,Ana Flávia; Renesto,Erasmo; Roxo,Fábio F.; Zawadzki,Cláudio H.
Allozyme electrophoresis was used to examine 12 enzymatic systems in two populations of the genus Neoplecostomus from the Paraná River basin. Samples of Neoplecostomus sp. 1 were collected in Paraitinguinha stream of the Tietê River basin, in the municipality of Salesópolis, São Paulo State, and those of Neoplecostomus sp. 2 from São Domingos stream of the Rio Grande River basin, in the municipality of Muzambinho, Minas Gerais State. The genetic variability of the two populations was estimated by Nei's expected heterozygosity and was considered lower than average for populations of freshwater fish. The proportion of polymorphic loci was low (only 5.26% for the locus Idh). The low frequency of heterozygosity for both populations revealed a high fixation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allozymes; Genetic variability; Neoplecostominae; Neotropical fishes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300021

What is new on plant cytogenetics?
Provedor de dados: Genetics and Molecular Biology
Autores: Guerra,Marcelo.
Tipo: Info:eu-repo/semantics/article
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300019

Translational selection on SHH genes
Provedor de dados: Genetics and Molecular Biology
Autores: Hajjari,Mohammadreza; Saffar,Behnaz; Khoshnevisan,Atefeh.
Codon usage bias has been observed in various organisms. In this study, the correlation between SHH genes expression in some tissues and codon usage features was analyzed by bioinformatics. We found that translational selection may act on compositional features of this set of genes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics; Codon usage bias; Correlation; Translational selection.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200033

Characterization of six microsatellite loci in  Myrica faya  (Myricaceae)   and cross amplification in the endangered endemic  M. rivas-martinezii    in Canary Islands, Spain
Provedor de dados: Genetics and Molecular Biology
Autores: González-Pérez,Miguel A.; Newton,Craig; Sosa,Pedro A.; Rivero,Elizabeth; González-González,Edna A.
Six novel polymorphic microsatellite markers were isolated from enriched libraries in  Myrica faya  Ait., recently renamed  Morella faya , (fayatree, firetree, or firebush) in order to examine the genetic diversity in natural populations. Also, test cross-specific amplification and genetic diversity in  Myrica rivas-martinezii,  which is endemic on the Canary islands. Microsatellite loci were screened in 225 individuals of both species from different islands of the Canarian archipelago. All markers were successfully amplified from both  Myrica  species, with an average number of 6.5 and 9.3 alleles per locus in  M. rivas-martinezii  and  M. faya , respectively. There was no evidence for linkage disequilibrium between loci, and the probability of null...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myrica rivas-martinezii; Myrica faya; Microsatellite; Canary Islands; Genetic diversity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100017

Leuciscus (Pisces, Cyprinidae) karyotypes: Transect of Portuguese populations
Provedor de dados: Genetics and Molecular Biology
Autores: Collares-Pereira,Maria João; Próspero,M.I.; Biléu,R.I.; Rodrigues,E.M.
The presently described Iberian chubs - Leuciscus carolitertii and L. pyrenaicus - sampled throughout their distribution ranges in Portugal were cytogenetically analyzed. Their chromosome numbers were consistently 2n = 50, except for two specimens of L. carolitertii, which exhibited a supernumerary chromosome in some of the metaphases. The karyotypes were found to be highly typical for other Leuciscus taxa, as well as for European leuciscine cyprinids: the chromosome sets are dominated by metacentric and submetacentric elements with a reduced number of acrocentric pairs (three to four); the largest pair of the complements belongs typically to this latter category. The chubs from northern drainages, assignable to L. carolitertii, have apparently a more...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100011

Comments on R.C. Guimarães' 'The systemic concept of the gene at age fifteen'
Provedor de dados: Genetics and Molecular Biology
Autores: El-Hani,Charbel Niño.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400034

Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis)
Provedor de dados: Genetics and Molecular Biology
Autores: Otaviano,Antonio Roberto; Tonhati,Humberto; Sena,Janete Aparecida Desidério; Cerón Muñoz,Mario Fernando.
Caseins comprise make up about 80% of the total protein content of milk and present polymorphism with changes in the amino acid sequence. Within this abundance of proteins, kappa-casein is noteworthy, since it has been associated with differences in milk yield, composition and processing. The objective of this study was to observe the existence of polymorphism in the kappa-casein gene in female buffaloes. For this purpose, blood samples from 115 female buffaloes, collected with vacutainer by needle punctionure of the jugular vein, were used. for genomic DNA extraction was done from blood samples. The PCR-RFLP and SSCP techniques demonstrated that the studied animals were monomorphic for the kappa-casein gene. Only allele B was observed in these animals,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buffaloes; Polymorphism; PCR-RFLP; SSCP.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200010

Spi2 gene polymorphism is not associated with recurrent airway obstruction and inflammatory airway disease in thoroughbred horses
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Aline Correa da; Brass,Karin Erica; Loreto,Elgion da Silva; Vinocur,Myriam Elizabeth; Pozzobon,Ricardo; Azevedo,Marcos da Silva.
The aim was to detect the presence of polymorphisms at exons 1, 2, 3 and 4 of the Spi2 gene, and evaluate a possible association between them and recurrent airway obstruction (RAO) or inflammatory airway disease (IAD) in thoroughbred horses, through single-strand conformational-polymorphism (SSCP) screening. Although polymorphism was not detected in exons 1, 2 and 3, three alleles and six genotypes were identified in exon 4. The frequencies of allele A (0.6388) and genotype AA (0.3888) were higher in horses affected by RAO, although no association was found between polymorphism and horses with either RAO or IAD.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse; Respiratory disease; SSCP; Alpha-1-antitrypsin; Serpin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300015

Expression of myogenes in longissimus dorsi muscle during prenatal development in commercial and local Piau pigs
Provedor de dados: Genetics and Molecular Biology
Autores: Reis,Evelyze Pinheiro dos; Paixão,Débora Martins; Brustolini,Otávio José Bernardes; Silva,Fabyano Fonseca e; Silva,Walmir; Araújo,Flávio Marcos Gomes de; Salim,Anna Christina de Matos; Oliveira,Guilherme; Guimarães,Simone Eliza Facioni.
Abstract This study used qRT-PCR to examine variation in the expression of 13 myogenes during muscle development in four prenatal periods (21, 40, 70 and 90 days post-insemination) in commercial (the three-way Duroc, Landrace and Large-White cross) and local Piau pig breeds that differ in muscle mass. There was no variation in the expression of the CHD8, EID2B, HIF1AN, IKBKB, RSPO3, SOX7 and SUFU genes at the various prenatal ages or between breeds. The MAP2K1 and RBM24 genes showed similar expression between commercial and Piau pigs but greater expression (p &lt; 0.05) in at least one prenatal period. Pair-wise comparisons of prenatal periods in each breed showed that only the CSRP3, LEF1, MRAS and MYOG genes had higher expression (p &lt; 0.05) in at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Commercial line; Gene expression; Myogenesis; Pig; Piau breed.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400589

Beta-globin gene cluster haplotypes in Venezuelan sickle cell patients from the State of Aragua
Provedor de dados: Genetics and Molecular Biology
Autores: Moreno,Nancy; Martínez,José A.; Blanco,Zorella; Osorio,Leidys; Hackshaw,Patrick.
Seven polymorphic sites in the beta-globin gene cluster were analyzed on a sample of 96 chromosomes of Venezuelan sickle cell patients from the State of Aragua. The Benin haplotype was predominant with a frequency of 0.479, followed by the Bantu haplotype (0.406); a minority of cases with other haplotypes was also identified: atypical Bantu A2 (0.042), Senegal (0.031), atypical Bantu A7 (0.021) and Saudi Arabia/Indian (0.021) haplotypes; however, the Cameroon haplotype was not identified in this study. Our results are in agreement with the historical records that establish Sudanese and Bantu origins for the African slaves brought into Venezuela.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haplotypes; Beta-globin gene cluster; Sickle cell.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100005

Assessment of genetic integrity, splenic phagocytosis and cell death potential of (Z)-4-((1,5-dimethyl-3-oxo-2-phenyl-2,3dihydro-1H-pyrazol-4-yl) amino)-4-oxobut-2-enoic acid and its effect when combined with commercial chemotherapeutics
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Rodrigo Juliano; da Cruz Leite Santos,Naiara; Pesarini,João Renato; de Oliveira,Beatriz Carneiro; Berno,Claudia Rodrigues; de Araújo,Flávio Henrique Souza; da Silveira,Ingridhy Ostaciana Maia Freitas; Nascimento,Raquel Oliveira; Brochado Antoniolli-Silva,Andréia Conceição Milan; Duenhas Monreal,Antônio Carlos; Beatriz,Adilson; de Lima,Dênis Pires; da Silva Gomes,Roberto.
Abstract The increased incidence of cancer and its high treatment costs have encouraged the search for new compounds to be used in adjuvant therapies for this disease. This study discloses the synthesis of (Z)-4-((1,5-dimethyl-3-oxo-2-phenyl-2,3dihydro-1H-pyrazol-4-yl) amino)-4-oxobut-2-enoic acid (IR-01) and evaluates not only the action of this compound on genetic integrity, increase in splenic phagocytosis and induction of cell death but also its effects in combination with the commercial chemotherapeutic agents doxorubicin, cisplatin and cyclophosphamide. IR-01 was designed and synthesized based on two multifunctionalyzed structural fragments: 4-aminoantipyrine, an active dipyrone metabolite, described as an antioxidant and anti-inflammatory agent; and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Splenic phagocytosis; Comet assay; Micronucleus test; Cell death; Chemoprevention.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100154

PACT/PRKRA and p53 regulate transcriptional activity of DMRT1
Provedor de dados: Genetics and Molecular Biology
Autores: Fujitani,Kazuko; Otomo,Asako; Nagayama,Yuto; Tachibana,Taro; Kato,Rika; Kawashima,Yusuke; Kodera,Yoshio; Kato,Tomoko; Takada,Shuji; Tamura,Kei; Takamatsu,Nobuhiko; Ito,Michihiko.
Abstract The transcription factor DMRT1 (doublesex and mab-3 related transcription factor) has two distinct functions, somatic-cell masculinization and germ-cell development in some vertebrate species, including mouse and the African clawed frog Xenopus laevis. However, its transcriptional regulation remains unclear. We tried to identify DMRT1-interacting proteins from X. laevis testes by immunoprecipitation with an anti-DMRT1 antibody and MS/MS analysis, and selected three proteins, including PACT/PRKRA (Interferon-inducible double-stranded RNA dependent protein kinase activator A) derived from testes. Next, we examined the effects of PACT/PRKRA and/or p53 on the transcriptional activity of DMRT1. In transfected 293T cells, PACT/PRKRA and p53...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P53; DMRT1; Germline stem cell; Testis; Xenopus.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400702

Characterization and expression analysis of WOX2 homeodomain transcription factor in Aegilops tauschii
Provedor de dados: Genetics and Molecular Biology
Autores: Zhao,Shan; Jiang,Qian-Tao; Ma,Jian; Wang,Ji-Rui; Liu,Ya-Xi; Chen,Guo-Yue; Qi,Peng-Fei; Pu,Zhi-En; Lu,Zhen-Xiang; Zheng,You-Liang; Wei,Yu-Ming.
The WUSCHEL (WUS)-related homeobox (WOX) gene family coordinates transcription during the early phases of embryogenesis. In this study, a putative WOX2 homolog was isolated and characterized from Aegilops tauschii, the donor of D genome of Triticum aestivum. The sequence consisted of 2045 bp, and contained an open reading frame (ORF), encoded 322 amino acids. The predicted protein sequence contained a highly conserved homeodomain and the WUS-box domain, which is present in some members of the WOX protein family. The full-length ORF was subcloned into prokaryotic expression vector pET-30a, and an approximately 34-kDa protein was expressed in Escherichia coli BL21 (DE3) cells with IPTG induction. The molecular mass of the expressed protein was identical to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: WOX2; Aegilops tauschii; Phylogenetic analysis; Real-time quantitative PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100079

In silico characterization of microRNAs-like sequences in the genome of Paracoccidioides brasiliensis
Provedor de dados: Genetics and Molecular Biology
Autores: Curcio,Juliana S. de; Batista,Mariana P.; Paccez,Juliano D.; Novaes,Evandro; Soares,Célia Maria de Almeida.
Abstract Eukaryotic cells have different mechanisms of post-transcriptional regulation. Among these mechanisms, microRNAs promote regulation of targets by cleavage or degradation of the mRNA. Fungi of the Paracoccidioides complex are the etiological agents of the main systemic mycosis of Latin America. These fungi present a plasticity to adapt and survive in different conditions, and the presence of microRNAs-like molecules could be part of the mechanisms that provide such plasticity. MicroRNAs produced by the host influence the progression of this mycosis in the lungs besides regulating targets involved in apoptosis in macrophage, activation of T and B cells and the production of cytokines. Therefore, this work analyzed the presence of regions in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungi; Mycoses; Dicer; Argonaute; MicroRNAs.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100095

Cytogenetic divergence between two sympatric species of Characidium (Teleostei, Characiformes, Crenuchidae) from the Machado River, Minas Gerais, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Alexandre Rodrigues da; Maistro,Edson Luis.
Cytogenetic studies were performed on two sympatric species of Characidium, C. gomesi and C. cf. zebra, from the Grande River basin, Minas Gerais State, Brazil. Although both species had a chromosome number of 50 with a karyotype exclusively consisting of meta- and submetacentric chromosomes, interspecific diversity was detected concerning the size of the two first chromosome pairs of the karyotypes. Active nucleolus organizer regions (NORs) were located at the terminal position on the long arm of the 17th pair of C. gomesi and at subterminal position on the long arm of the 23rd pair of C. cf. zebra. For both species the fluorochrome CMA3 stained only the NOR-bearing pair of chromosomes. The heterochromatin pattern also showed some differentiation between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characidium gomesi; Characidium cf. zebra; Crenuchidae; NOR-banding; C-banding.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300010

Impact of a chromosome X STR Decaplex in deficiency paternity cases
Provedor de dados: Genetics and Molecular Biology
Autores: Trindade-Filho,Aluisio; Ferreira,Samuel; Oliveira,Silviene F.
Deficiency paternity cases, characterized by the absence of the alleged father, are a challenge for forensic genetics. Here we present four cases with a female child and a deceased alleged father in which the analysis of a set of 21 or 22 autosomal STRs (AS STRs) produced results within a range of doubt when genotyping relatives of the alleged father. Aiming to increase the Paternity Index (PI) and obtain more reliable results, a set of 10 X-linked STR markers, developed by the Spanish and Portuguese Group of the International Society for Forensic Genetics (ISFG), was then added. Statistical analysis substantially shifted the results towards the alleged fatherhood in all four cases, with more dramatic changes when the supposed half-sister and respective...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Forensic genetics; ChrX STR Decaplex; X-linked markers; Deficiency paternity case; Paternity Index.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400008

Erratum
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200032

Association study of folate-related enzymes (MTHFR, MTR, MTRR) genetic variants with non-obstructive male infertility in a Polish population
Provedor de dados: Genetics and Molecular Biology
Autores: Kurzawski,Mateusz; Wajda,Anna; Malinowski,Damian; Kazienko,Anna; Kurzawa,Rafal; Drozdzik,Marek.
Spermatogenesis is a process where an important contribution of genes involved in folate-mediated one-carbon metabolism is observed. The aim of the present study was to investigate the association between male infertility and the MTHFR (677C &gt; T; 1298A &gt; C), MTR (2756A &gt; G) and MTRR (66A &gt; G) polymorphisms in a Polish population. No significant differences in genotype or allele frequencies were detected between the groups of 284 infertile men and of 352 fertile controls. These results demonstrate that common polymorphisms in folate pathway genes are not major risk factors for non-obstructive male infertility in the Polish population.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: MTHFR; MTR; MTRR; Polymorphism; Infertility.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100042

Glutathione depletion triggers actin cytoskeleton changes via actin-binding proteins
Provedor de dados: Genetics and Molecular Biology
Autores: Zepeta-Flores,Nahum; Valverde,Mahara; Lopez-Saavedra,Alejandro; Rojas,Emilio.
Abstract The importance of glutathione (GSH) in alternative cellular roles to the canonically proposed, were analyzed in a model unable to synthesize GSH. Gene expression analysis shows that the regulation of the actin cytoskeleton pathway is strongly impacted by the absence of GSH. To test this hypothesis, we evaluate the effect of GSH depletion via buthionine sulfoximine (5 and 12.5 mM) in human neuroblastoma MSN cells. In the present study, 70% of GSH reduction did not induce reactive oxygen species, lipoperoxidation, or cytotoxicity, which enabled us to evaluate the effect of glutathione in the absence of oxidative stress. The cells with decreasing GSH levels acquired morphology changes that depended on the actin cytoskeleton and not on tubulin. We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione; BSO; Thymosin β4; Gelsolin; Profiling.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300475

Comparative transcriptomic analysis of two important life stages of Angiostrongylus cantonensis: fifth-stage larvae and female adults
Provedor de dados: Genetics and Molecular Biology
Autores: Yu,Liang; Cao,Binbin; Long,Ying; Tukayo,Meks; Feng,Chonglv; Fang,Wenzhen; Luo,Damin.
Abstract The mechanisms involved in the fast growth of Angiostrongylus cantonensis from fifth-stage larvae (L5) to female adults and how L5 breaks through the blood-brain barrier in a permissive host remain unclear. In this work, we compared the transcriptomes of these two life stages to identify the main factors involved in the rapid growth and transition to adulthood. RNA samples from the two stages were sequenced and assembled de novo. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses of 1,346 differentially expressed genes between L5 and female adults was then undertaken. Based on a combination of analytical results and developmental characteristics, we suggest that A. cantonensis synthesizes a large amount of cuticle in L5 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comparative transcriptomes; Cuticle synthesis; Lysine degradation; Lysosomal pathway; Metallopeptidases.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300540

Resistance to Anticarsia gemmatalis Hübner (Lepidoptera, Noctuidae) in transgenic soybean (Glycine max (L.) Merrill Fabales, Fabaceae) cultivar IAS5 expressing a modified Cry1Ac endotoxin
Provedor de dados: Genetics and Molecular Biology
Autores: Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Pasquali,Giancarlo; Zaidi,Mohsin Abbas; Altosaar,Illimar; Bodanese-Zanettini,Maria Helena.
Somatic embryos of the commercial soybean (Glycine max) cultivar IAS5 were co-transformed using particle bombardment with a synthetic form of the Bacillus thuringiensis delta-endotoxin crystal protein gene cry1Ac, the beta-glucuronidase reporter gene gusA and the hygromycin resistance gene hpt. Hygromycin-resistant tissues were proliferated individually to give rise to nine sets of clones corresponding to independent transformation events. The co-bombardment resulted in a co-transformation efficiency of 44%. Many histodifferentiated embryos and 30 well-developed plants were obtained. Twenty of these plants flowered and fourteen set seeds. The integration and expression of the cry1Ac, gusA and hpt transgenes into the genomes of a sample of transformed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticarsia gemmatalis; Cry1Ac gene; IAS5 cultivar; Insect resistance; Transgenic soybean.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300020

Characterization and copy number of the S27 ribosomal protein gene from amphioxus Branchiostoma belcheri tsingtauense
Provedor de dados: Genetics and Molecular Biology
Autores: Ma,Lifang; Zhang,Shicui; Liu,Zhenhui; Li,Hongyan; Xia,Jianjun.
A cDNA clone encoding ribosomal protein S27 (AmphiS27) was identified in the gut cDNA library of amphioxus Branchiostoma belcheri tsingtauense. This cDNA consists of 607 bp and contains a 255 bp open reading frame (ORF) corresponding to a deduced protein of 84 amino acids with a calculated molecular mass of 9,488 Da and an isoelectric point (pI) of 9.500. Alignment of the deduced AmphiS27 amino acid sequence with 12 known S27 protein sequences indicates that AmphiS27 shares 94-99% homology with its vertebrate homologue, 84-94% with invertebrate homologues and 69-72% with homologues from other eukaryotes, suggesting that AmphiS27 is more closely related to the vertebrate S27 protein than to its invertebrate counterpart. Southern blot analysis showed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphioxus; Branchiostoma; Ribosomal protein; S27; Copy number.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500029

Scattered organization of the histone multigene family and transposable elements in Synbranchus
Provedor de dados: Genetics and Molecular Biology
Autores: Utsunomia,Ricardo; Pansonato-Alves,José Carlos; Scacchetti,Priscilla Cardim; Oliveira,Claudio; Foresti,Fausto.
The fish species Synbranchus marmoratus is widely distributed throughout the Neotropical region and exhibits a significant karyotype differentiation. However, data concerning the organization and location of the repetitive DNA sequences in the genomes of these karyomorphs are still lacking. In this study we made a physical mapping of the H3 and H4 histone multigene family and the transposable elements Rex1 and Rex3 in the genome of three known S. marmoratus karyomorphs. The results indicated that both histone sequences seem to be linked with one another and are scattered all over the chromosomes of the complement, with a little compartmentalization in one acrocentric pair, which is different from observations in other fish groups. Likewise, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synbranchidae; FISH; Histone; Retrotransposon.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100007

The natural history of pregnancies with prenatal diagnosis of Trisomy 18 or Trisomy 13: Retrospective cases of a 23-year experience in a Brazilian public hospital
Provedor de dados: Genetics and Molecular Biology
Autores: Duque,Julio Alejandro Peña; Ferreira,Charles Francisco; Zachia,Suzana de Azevedo; Sanseverino,Maria Teresa Vieira; Gus,Rejane; Magalhães,José Antônio de Azevedo.
Abstract Trisomy 18 (T18) and trisomy 13 (T13) are polymalformative syndromes associated with a high rate of spontaneous abortions, intrauterine death, and short postnatal life. This study describes the overall outcome in a country where the therapeutic interruption of pregnancy is not available. The medical records of women with prenatal diagnosis of full trisomy of T13 or T18 between October 1994 and October 2017 were analyzed in order to describe their natural outcomes. Thirteen cases of T13 and 29 cases of T18 were included. The miscarriage rate was 9% for T18 and no cases for T13. Intrauterine fetal death occurred in 46% and 52% of cases for T13 and T18, respectively. The rate of live births for T13 was 54%, and the median survival was one day (95% CI...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Natural history of trisomy; Trisomy 13; Trisomy 18; Prenatal diagnosis; Genetic counseling.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200286

Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Lopes,Denilce Meneses; Campos,Lúcio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.
Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melipona; Stingless bees; Microsatellite; Transferability.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200030

A useful routine for biochemical detection and diagnosis of mucopolysaccharidoses
Provedor de dados: Genetics and Molecular Biology
Autores: Leistner,Sandra; Giugliani,Roberto.
Mucopolysaccharidoses (MPS) constitute, owing to their biochemical, genetical and clinical characteristics, a large and heterogeneous subgroup among the lysosomal storage diseases (LSD). They are caused by deficiency of specific enzymes, which are responsible for glycosaminoglycan (GAG) breakdown during different steps of its degradation pathway. MPS are responsible for about 32% of inborn errors of metabolism (IEM) and 54% of LSD identified in our laboratory (Regional Laboratory of Inborn Errors of Metabolism (RLIEM), Medical Genetics Unit, Hospital de Clínicas in Porto Alegre), which is a reference center for LSD diagnosis in Brazil. Therefore, we decided to set up a specific laboratory routine for detection and differential diagnosis of MPS in patients...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100028

Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)
Provedor de dados: Genetics and Molecular Biology
Autores: Kumar,Dinesh; Gupta,Neelam; Ahlawat,S.P.S.; Satyanarayana,R.; Sunder,Shyam; Gupta,S.C.
The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect &lt;FONT FACE=Symbol&gt;a&lt;/font&gt;-lactalbumin polymorphism in a sample of 50 goats. Our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability in Jamunapari goats. Polymorphism was detected in the sample, indicating that Jamunapari goats have high genetic variability at loci, exon I of the a-lactalbumin gene. This result opens interesting prospects for future selection programs and conservation strategies. These a-lactalbumin variants can be sequenced and screened in the population to develop single nucleotide polymorphism...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-lactalbumin; Genetic variability; Goat; Single-strand conformation polymorphism (SSCP).
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200016

Reviewing the characteristics of BRCA and PALB2-related cancers in the precision medicine era
Provedor de dados: Genetics and Molecular Biology
Autores: Macedo,Gabriel S.; Alemar,Barbara; Ashton-Prolla,Patricia.
Abstract Germline mutations in BRCA1 and BRCA2 (BRCA) genes confer high risk of developing cancer, especially breast and ovarian tumors. Since the cloning of these tumor suppressor genes over two decades ago, a significant amount of research has been done. Most recently, monoallelic loss-of-function mutations in PALB2 have also been shown to increase the risk of breast cancer. The identification of BRCA1, BRCA2 and PALB2 as proteins involved in DNA double-strand break repair by homologous recombination and of the impact of complete loss of BRCA1 or BRCA2 within tumors have allowed the development of novel therapeutic approaches for patients with germline or somatic mutations in said genes. Despite the advances, especially in the clinical use of PARP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BRCA1; BRCA2; Homologous recombination; Cancer predisposition; PARP inhibitors.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200215

Evaluation of the association between the TAS1R2 and TAS1R3 variants and food intake and nutritional status in children
Provedor de dados: Genetics and Molecular Biology
Autores: Melo,Silvia V.; Agnes,Grasiela; Vitolo,Márcia R.; Mattevi,Vanessa S.; Campagnolo,Paula D.B.; Almeida,Silvana.
Abstract Taste perception plays a key role in determining individual food preferences and dietary habits and may influence nutritional status. This study aimed to investigate the association of TAS1R2 (Ile191Val - rs35874116) and TAS1R3 (-1266 C/T - rs35744813) variants with food intake and nutritional status in children followed from birth until 7.7 years old. The nutritional status and food intake data of 312 children were collected at three developmental stages (1, 3.9 and 7.7 years old). DNA was extracted from blood samples and the polymorphisms were analyzed by real-time polymerase chain reactions (qPCR) using hydrolysis probes as the detection method. Food intake and nutritional status were compared among individuals with different single nucleotide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food intake; Polymorphism; TAS1R2 gene; TAS1R3 gene; Taste receptors.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300415

Differentially expressed stress-related genes in the compatible citrus-Citrus leprosis virus interaction
Provedor de dados: Genetics and Molecular Biology
Autores: Freitas-Astúa,Juliana; Bastianel,Marinês; Locali-Fabris,Eliane C.; Novelli,Valdenice M.; Silva-Pinhati,Ana Carla; Basílio-Palmieri,Ana Carolina; Targon,Maria Luisa P.N.; Machado,Marcos A.
Leprosis, caused by Citrus leprosis virus, cytoplasmic type (CiLV-C), is the main viral disease in the Brazilian citrus industry. This occurs because of the widespread source of inoculum and the year-round presence of the vector, the tenuipalpid mite Brevipalpus phoenicis, in citrus plants. In addition, while some Citrus species are resistant to CiLV-C, C. sinensis, the main cultivated species in the country, is extremely susceptible to the disease. The main objective of this work was to identify genes in C. sinensis cv. Pêra plants that were differentially expressed after the host was challenged with CiLV-C. In order to accomplish that, cDNA libraries were constructed from healthy and CiLV-inoculated sweet orange leaves. Two hundred and fifty-four genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EST; Disease; CiLV; In silico hybridization; Sweet orange; Citrus sinensis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500026

Prevalence of 185delAG and 5382insC mutations in BRCA1, and 6174delT in BRCA2 in women of Ashkenazi Jewish origin in southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Dillenburg,Crisle Vignol; Bandeira,Isabel Cristina; Tubino,Taiana Valente; Rossato,Luciana Grazziotin; Dias,Eleonora Souza; Bittelbrunn,Ana Cristina; Leistner-Segal,Sandra.
Certain mutations in BRCA1 and BRCA2 genes are frequent in the Ashkenazi Jewish population. Several factors contribute to this increased frequency, including consanguineous marriages and an event known as a "bottleneck', which occurred in the past and caused a drastic reduction in the genetic variability of this population. Several studies were performed over the years in an attempt to elucidate the role of BRCA1 and BRCA2 genes in susceptibility to breast cancer. The aim of this study was to estimate the carrier frequency of certain common mutations in the BRCA1 (185delAG and 5382insC) and BRCA2 (6174delT) genes in an Ashkenazi Jewish population from Porto Alegre, Brazil. Molecular analyses were done by PCR followed by RFLP (ACRS). The carrier frequencies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ashkenazi Jews; Breast cancer; BRCA1; BRCA2; Mutation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400009

Cytogenetics and immature embryo culture at Embrapa Trigo breeding program: transfer of disease resistance from related species by artificial resynthesis of hexaploid wheat (Triticum aestivum L. em. Thell)
Provedor de dados: Genetics and Molecular Biology
Autores: Fernandes,Maria Irene Baggio de Moraes; Zanatta,Ana Christina A.; Prestes,Ariano Moraes; Caetano,Vanderlei da Rosa; Barcellos,Amarilis Labes; Angra,Dilma Cristina; Pandolfi,Valesca.
Worldwide wheat (Triticum aestivum L. em. Thell, 2n = 6X = 42, AABBDD) breeding programs aim to reorganize genotypes to achieve better yields, environmental adaptation and food quality. The necessary interdisciplinarity for breeding purposes requires an accurate choice of the most appropriate cellular and/or molecular strategies available to be integrated with agronomic approaches in order to overcome the genetic limitation of each cultivated species, at each agroecosystem. Cytogenetics has given a great contribution to wheat genetic studies and breeding, due to viability of chromosomal variants because of homoeology among genomes in this allohexaploid species and the genus Triticum. The level of development of cytogenetic techniques achieved over the last...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400051

AFLP marker analysis revealing genetic structure of the tree Parapiptadenia rigida (Benth.) Brenan (Leguminosae-Mimosoideae) in the southern Brazilian Tropical Rainforest
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Laís Bérgamo de; Ruas,Eduardo A.; Rodrigues,Luana A.; Ruas,Claudete F.; Ruas,Paulo M.
Parapiptadenia rigida is a tropical early secondary succession tree characteristic of the Tropical Atlantic Rainforest. This species is of great ecological importance in the recovery of degraded areas. In this study we investigated the variability and population genetic structure of eight populations of P. rigida. Five AFLP primer combinations were used in a sample of 159 individuals representing these eight populations, rendering a total of 126 polymorphic fragments. The averages of percentage of polymorphic loci, gene diversity, and Shannon index were 60.45%, 0.217, and 0.322, respectively. A significant correlation between the population genetic variability and the population sizes was observed. The genetic variability within populations (72.20%) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tropical tree; Genetic diversity; Population genetics; Conservation; AFLP.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400012

Approaches for in silico finishing of microbial genome sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Kremer,Frederico Schmitt; McBride,Alan John Alexander; Pinto,Luciano da Silva.
Abstract The introduction of next-generation sequencing (NGS) had a significant effect on the availability of genomic information, leading to an increase in the number of sequenced genomes from a large spectrum of organisms. Unfortunately, due to the limitations implied by the short-read sequencing platforms, most of these newly sequenced genomes remained as “drafts”, incomplete representations of the whole genetic content. The previous genome sequencing studies indicated that finishing a genome sequenced by NGS, even bacteria, may require additional sequencing to fill the gaps, making the entire process very expensive. As such, several in silico approaches have been developed to optimize the genome assemblies and facilitate the finishing process. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial genetics; Molecular microbiology; Genomics; Microbiology; Draft genomes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400553

Citrus plastid-related gene profiling based on expressed sequence tag analyses
Provedor de dados: Genetics and Molecular Biology
Autores: Calsa Jr.,Tercilio; Figueira,Antonio.
Plastid-related sequences, derived from putative nuclear or plastome genes, were searched in a large collection of expressed sequence tags (ESTs) and genomic sequences from the Citrus Biotechnology initiative in Brazil. The identified putative Citrus chloroplast gene sequences were compared to those from Arabidopsis, Eucalyptus and Pinus. Differential expression profiling for plastid-directed nuclear-encoded proteins and photosynthesis-related gene expression variation between Citrus sinensis and Citrus reticulata, when inoculated or not with Xylella fastidiosa, were also analyzed. Presumed Citrus plastome regions were more similar to Eucalyptus. Some putative genes appeared to be preferentially expressed in vegetative tissues (leaves and bark) or in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus variegated chlorosis; In silico; Sweet orange; Tangerine.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500013

Evaluation of the mutagenic potential of Cochlospermum regium in Drosophila melanogaster male germ cells
Provedor de dados: Genetics and Molecular Biology
Autores: Nunes,Wanderlene Blanco; Carvalho,Salvador de.
During the last few decades the search for medical treatments based on alternative medicine has increased significantly, making knowledge of the plants commonly used as folk medicines extremely important. The plant Cochlospermum regium, a member of the Cochlospermaceae found in the Brazilian cerrado (a type of savanna), is known to have high depurative activity and to be effective not only in treating skin problems such as pimples, boils and blotches but also in curing gastritis and ulcers. We prepared aqueous extracts using 13, 19 and 25 gL-1 of dried C. regium root and investigated these extracts for possible mutagenic effects on Drosophila melanogaster germ cells. Mutagenesis was assessed using the ring-X loss (RXL) test which can detect chromosome...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutagenic potential; Ring-X-loss; Cochlospermum regium; Drosophila melanogaste.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400020

Lack of association between genetic polymorphisms in IGF1 and IGFBP3 with twin births in a Brazilian population (Cândido Godói, Rio Grande do Sul)
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira-Klein,Mariana de; Cardoso-dos-Santos,Augusto César; Tagliani-Ribeiro,Alice; Fagundes,Nelson Rosa; Matte,Ursula; Schuler-Faccini,Lavinia.
Abstract Insulin-like growth factor (IGF-1) is an important peptide hormone involved in the reproduction and fetal development of mammals, and it is suggested that it may influence the human twinning rate. This study aimed to test such possible association, investigating the genetic polymorphisms IGF1 (CA)n and IGFBP3 rs2854744 in the population from Candido Godoi (CG), a small city located in the South of Brazil that has a high prevalence of twin births. A case-control study was performed comprising a total of 39 cases (representing about 40% of the mothers of twins who were born in CG after 1995) and 214 controls (mothers of non-twin children), 97 of whom were living in CG while 117 were living in Porto Alegre. DNA was extracted from blood leucocytes and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin-like growth factor; Founder effect; Microsatellite; Reproduction; Twinning.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500775

Genetic relationships among Arachis species based on AFLP
Provedor de dados: Genetics and Molecular Biology
Autores: Gimenes,Marcos A.; Lopes,Catalina R.; Valls,Jose F.M.
Amplified Fragment Length Polymorphism (AFLP) was used to establish the genetic relationships among 20 species from seven of the nine sections of genus Arachis. The level of polymorphism among nine accessions of the cultivated peanut, A. hypogaea L., was also evaluated. Three combinations of primers were used to amplify the AFLPs. The fragments were separated in 6% denaturing acrylamide gels. A total of 408 fragments were analyzed. An average of 135.3 fragments per primer combination were scored, and the largest number of fragments was 169 using primer combination Eco RI - ACC / Mse I - CTG, while the lowest was 108, with Eco RI - ACT / Mse I - CTT. In general, the genetic relationships established using AFLPs agreed with the classification established...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Arachis; Peanut; Taxonomic relationship.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300017

Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus)
Provedor de dados: Genetics and Molecular Biology
Autores: Presti,Flavia T.; Meyer,Janaína; Antas,Paulo T.Z.; Guedes,Neiva M.R.; Miyaki,Cristina Y.
Molted feather sampling is a useful tool for genetic analyses of endangered species, but it is often very laborious due to the low quality and quantity of the DNA obtained. In the present study we show the parts of feathers that resulted in better yield of DNA. In descending order these were: blood clot outside the umbilicus, umbilicus (without blood clot), tip, inner membrane, and small calamus. Compared to DNA extracted from blood samples, DNA extracted from feathers produced microsatellite alleles of poorer quality and had to be processed immediately after extraction. As expected due to the level of DNA degradation, molecular sexing protocols that result in shorter PCR products were more efficient.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molted feathers; Blood sample; DNA; PCR product; Parrot.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100019

Replication of TCF7L2 rs7903146 association with type 2 diabetes in an Iranian population
Provedor de dados: Genetics and Molecular Biology
Autores: Amoli,Mahsa M.; Amiri,Parvin; Tavakkoly-Bazzaz,Javad; Charmchi,Elham; Hafeziyeh,Jila; Keramatipour,Mohammad; Abiri,Maryam; Ranjbar,Shirin Hasani; Larijani,Bagher.
The transcription factor 7-like 2 gene (TCF7L2) rs7903146 T allele is constantly associated with Type 2 diabetes in various populations and ethnic groups. Nevertheless, this has not been observed in two studies involving Arab populations. The aim of the present study was to investigate the association between TCF7L2 rs7903146 in an Iranian population. Type 2 diabetes patients (N = 258) and normal healthy control subjects (N = 168) from the same area, were examined. The ARMS-PCR (Amplification Refractory Mutation System) technique, subsequently validated by direct sequencing, was used for genotyping. Allele and genotype frequencies were significantly different between patients and controls TT vs. CT + CC [p 0.0081 OR 3.4 95%CI (1.27-11.9)] and T vs. C...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TCF7L2; Gene; Polymorphism; T2DM.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300010

De novo transcriptome assembly of the lobster cockroach Nauphoeta cinerea (Blaberidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Segatto,Ana Lúcia Anversa; Diesel,José Francisco; Loreto,Elgion Lucio Silva; da Rocha,João Batista Teixeira.
Abstract The use of Drosophila as a scientific model is well established, but the use of cockroaches as experimental organisms has been increasing, mainly in toxicology research. Nauphoeta cinerea is one of the species that has been studied, and among its advantages is its easy laboratory maintenance. However, a limited amount of genetic data about N. cinerea is available, impeding gene identification and expression analyses, genetic manipulation, and a deeper understanding of its functional biology. Here we describe the N. cinerea fat body and head transcriptome, in order to provide a database of genetic sequences to better understand the metabolic role of these tissues, and describe detoxification and stress response genes. After removing low-quality...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nauphoeta cinerea; Transcriptome assembly; Fat body; Detoxification.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400713

Haplotype diversity of 17 Y-str loci in an admixed population from the Brazilian Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Francez,Pablo Abdon da Costa; Ramos,Luiz Patrick Vidal; Palha,Teresinha de Jesus Brabo Ferreira; Santos,Sidney Emanuel Batista dos.
The allelic and haplotype frequencies of 17 Y-STR loci most commonly used in forensic testing were estimated in a sample of 138 unrelated healthy males from Macapá, in the northern Amazon region of Brazil. The average gene diversity was 0.6554 ± 0.3315. 134 haplotypes of the 17 loci were observed, 130 of them unique and four present in two individuals each. The haplotype diversity index was 0.9996 + 0.0009, with the most frequent haplogroups being R1b (52.2%), E1b1b (11.6%), J2 (10.1%) and Q (7.2%). Most haplogroups of this population belonged to European male lineages (89.2%), followed by Amerindian (7.2%) and African (3.6%) lineages.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon population; Forensic genetics; Haplogroups; Y-haplotypes; Y-STR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100006

Studies of blood groups and protein polymorphisms in the Brazilian horse breeds Mangalarga Marchador and Mangalarga (Equus caballus)
Provedor de dados: Genetics and Molecular Biology
Autores: Lippi,Andréia Samaha; Mortari,Norma.
Allelic frequencies at 12 loci (five blood groups: C, D, K, P, and U; and seven protein polymorphisms: Al, A1B, Es, Gc, Hb, PGD, and Tf), are given for two Brazilian horse breeds: Mangalarga Marchador and Mangalarga. The high genetic identity value found (96.0%) is consistent with their common origin, although, at some point of the development of Mangalarga Marchador, Mangalarga separated from the original stock. The expected average heterozygosity was higher in Mangalarga Marchador. The populations presented genetic differentiation, as shown by the statistically significant value of F ST. The nonsignificant F IS values showed that there was no appreciable consanguineous mating in any of the two populations. Exclusion probability calculated for the 12 loci...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood groups; Biochemical polymorphisms; Breeds; Horses.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400005

Analysis of the population structure of Uruguayan Creole cattle as inferred from milk major gene polymorphisms
Provedor de dados: Genetics and Molecular Biology
Autores: Rincón,Gonzalo; Armstrong,Eileen; Postiglioni,Alicia.
The ancestors of Uruguayan Creole cattle were introduced by the Spanish conquerors in the XVII century, following which the population grew extensively and became semi-feral before the introduction of selected breeds. Today the Uruguayan Creole cattle genetic reserve consists of 575 animals. We used the tetra primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR) to analyze the kappa-casein, beta-casein, alphaS1-casein and alpha-lactoalbumin gene polymorphisms and restriction fragment length polymorphism PCR (RFLP-PCR) for the beta-lactoglobulin and the acylCoA:diacyl glycerol acyltransferase 1 (DGAT1) genes. The kappa-casein and beta-lactoglobulin genes presented very similar A and B allele frequencies, while the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DGAT1 gene; Milk protein; SNPs; Uruguayan Creole cattle.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300016

Genome analysis of Mexican flora
Provedor de dados: Genetics and Molecular Biology
Autores: Palomino,Guadalupe.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400036

Esterase-D and chromosome patterns in Central Amazon piranha (Serrasalmus rhombeus Linnaeus, 1766) from Lake Catalão
Provedor de dados: Genetics and Molecular Biology
Autores: Teixeira,Aylton Saturnino; Nakayama,Celeste Mutuko; Porto,Jorge Ivan Rebelo; Feldberg,Eliana.
This study presents additional genetic data on piranha (Serrasalmus rhombeus Linnaeus, 1766) complex previously diagnosed due to the presence of distinct cytotypes 2n = 58 and 2n = 60. Three esterase-D enzyme loci (Est-D1, Est-D2 and Est-D3) were examined and complemented with chromosomal data from 66 piranha specimens collected from Lake Catalão. For all specimens the Est-D1 and Est-D2 loci were monomorphic. In contrast, the Est-D3 locus was polymorphic with genotypes and alleles being differentially distributed in the previously described cytotypes and served as the basis for detecting a new cytotype (2n = 60 B). In cytotype 2n = 58 the Est-D3 locus was also polymorphic and presented Mendelian allelic segregation with four genotypes (Est-D3(11),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian Amazon basin; Esterase enzymes; Serrasalmus rhombeus species complex; Karyotype.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300018

Differential effects of the methylenetetrahydrofolate reductase polymorphisms (C677T and A1298C) on hematological malignancies among Latinos: a meta-analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Garcia-Hernandez,Samanta Celeste; Meneses-Sanchez,Perla; Porchia,Leonardo Martin; Torres-Rasgado,Enrique; Pérez-Fuentes,Ricardo; Gonzalez-Mejia,Martha Elba.
Abstract Our objective was to determine the association between the methylenetetrahydrofolate reductase polymorphisms (C677T and A1298C) and the risk of developing acute lymphoblastic leukemia (ALL), chronic myeloid leukemia (CML), acute myeloid leukemia (AML), and multiple myelomas (MM) in Latinos. PubMed, SCOPUS, EBSCO, LILACS, and other Latin-specific databases were searched for case-control studies that investigated the association between these polymorphisms and hematologic malignancies until November 2017. Genotype distributions were extracted and either fixed-effects or random-effects models were used to calculate the pooled crude odds ratios (ORs) for the heterozygous, homozygous, dominant, recessive, and allelic genetic models. No publication bias...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MTHFR; Protective factor; Latin America; Leukemia.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400549

Genetic diversity in populations of African mahogany (Khaya grandioliola C. DC.) introduced in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Soares,Sabrina Delgado; Bandeira,Ludmila Ferreira; Ribeiro,Stela Barros; Telles,Mariana Pires de Campos; Silva,João Augusto da; Borges,Canrobert Tormin; Coelho,Alexandre Siqueira Guedes; Novaes,Evandro.
Abstract Given its high-valued wood, the African mahogany (Khaya grandifoliola) has been envisaged as a renewable source of tropical hardwoods in Brazil. However, there are concerns about the hypothesized low diversity among the few K. grandifoliola germplasm sources introduced in the country. Using eight microsatellite markers, we evaluated the genetic diversity and divergence among 53 superior trees selected from three provenances of K. grandifoliola located in the state of Para. These populations are among the oldest plantations (&gt;15 years) in Brazil and, therefore, the country's main seed sources. The average number of alleles per locus was 5.9, expected heterozygosity was moderate (^=0.56) and lower than the high observed heterozygosity (HO=0.74)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; SSR; Genetic structure; Genetic divergence; Hardwood.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400301

The complete mitochondrial genome sequence of the little egret (Egretta garzetta)
Provedor de dados: Genetics and Molecular Biology
Autores: Zou,Yi; Jing,Mei-dong; Bi,Xiao-xin; Zhang,Ting; Huang,Ling.
Many phylogenetic questions in the Ciconiiformes remain unresolved and complete mitogenome data are urgently needed for further molecular investigation. In this work, we determined the complete mitogenome sequence of the little egret (Egretta garzetta). The genome was 17,361 bp in length and the gene organization was typical of other avian mtDNA. In protein-coding genes (PCGs), a C insertion was found in ND3, and CO III and ND4 terminated with incomplete stop codons (T). tRNA-Val and tRNA-Ser (AGY) were unable to fold into canonical cloverleaf secondary structures because they had lost the DHU arms. Long repetitive sequences consisting of five types of tandem repeats were found at the 3′ end of Domain III in the control region. A phylogenetic analysis of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Egretta garzetta; Mitochondrial genome; Phylogenomics.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200162

Molecular characterization of a cryptic wheat-Thinopyrum intermedium translocation line: evidence for genomic instability in nascent allopolyploid and aneuploid lines
Provedor de dados: Genetics and Molecular Biology
Autores: Yingshan,Dong; Xiuling,Bu; Yushi,Luan; Mengyuan,He; Bao,Liu.
A putative translocation line (#32), together with a disomic addition line (TAI27) and an octo-amphiploid line (Zhong3) of common wheat and Thinopyrum intermedium were characterized by restriction fragment length polymorphism (RFLP) analysis, using probes covering all seven homoeologous groups of Triticeae. Line 32 was confirmed to be a cryptic translocation line, based on the detection of multiple introgressed hybridization fragments specific to Th. intermedium in the RFLP patterns, and the absence of a hybridization signal in GISH analysis. In addition, extensive genomic changes, as compared to the wheat parent, were detected on all three lines studied, with a great majority of changes showing concordance among the lines. Our data is consistent with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Translocation; Genomic changes; Allopolyploidy and aneuploidy; Wheat; Thinopyrum intermedium.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200018

The use of PCR-RFLP as an identification tool for two closely related species of bats of genus Platyrrhinus
Provedor de dados: Genetics and Molecular Biology
Autores: Ferreira,Juliana Machado; Martins,Felipe de Melo; Ditchfield,Albert; Morgante,João Stenghel.
The bat species Platyrrhinus lineatus and P. recifinus (Phyllostomidae: Stenodermatinae) are ecologically important because of their capacity for seed dispersal. P. recifinus is endemic to the Atlantic rain forest and is considered vulnerable by the IUCN. The lack of distinct morphological features makes identification of the two species a difficult task. This study was aimed at testing the hypothesis that these are actually two distinct species by using PCR-RFLP of the mitochondrial cytocrome b gene. The results showed no shared haplotypes, demonstrating that these are, in fact, two distinct species. No polymorphism was obtained for P. recifinus, which could be a sign of low genetic diversity in this threatened species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Platyrrhinus; PCR-RFLP; Identification; Phyllostomidae.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100021

OppA Escherichia coli mutants have osmodependent resistance to aminoglycoside antibiotics
Provedor de dados: Genetics and Molecular Biology
Autores: Tsuhako,Maria H.; Ferreira,Luis Carlos S.; Costa,Sérgio Olavo P. da.
The oligopeptide permease (OppA) protein was found to be missing in the periplasmic fractions of Escherichia coli kanamycin-resistant mutants selected under high osmotic conditions. The growth behavior of one mutant in media containing kanamycin or the toxic peptide triornithine suggests that OppA and another cell envelope component contribute to the osmolarity-dependent aminoglycoside resistance of E. Coli.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100004

Determination of genetic variation in Rhodiola crenulata from the Hengduan Mountains Region, China using inter-simple sequence repeats
Provedor de dados: Genetics and Molecular Biology
Autores: Lei,Yidong; Gao,Hong; Tsering,Tashi; Shi,Suhua; Zhong,Yang.
The plant Rhodiola crenulata is a perennial herbaceous species distributed in the plateau region of southwestern China, especially the Hengduan Mountains region. It has been one of the most important traditional herbal remedies in Tibet for more than one thousand years, but the accelerated and uncontrolled collection of this plant since the 1980s has lead to deforestation. We used inter-simple sequence repeats (ISSR) to assess levels of genetic variation in R. crenulata from nine diverse natural populations in eastern Tibet and northern Yunnan, the first time such a study has been carried out. The 12 primers we used were able to detect 184 polymorphic loc. Analysis of molecular variance (AMOVA) indicated that species level genetic diversity was relatively...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Hengduan Mountains region; ISSR; Rhodiola crenulata.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200023

Evolutionary dynamics of the karyotype of the wasp Trypoxylon (Trypargilum) nitidum (Hymenoptera, Sphecidae) from the Rio Doce State Park, Minas Gerais, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Scher,Ricardo; Pompolo,Silvia das Graças.
Cytogenetic analysis based on the distribution of C-bands showed two groups of karyotypes in a Trypoxylon nitidum population from the Rio Doce Park, State of Minas Gerais, Brazil. One of these groups, that was identical to a previously described karyotype (n = 15; 2n = 30), had a stable chromosome number and was rich in acrocentric chromosomes, whereas the other had a variable chromosome number (n = 12 to 14; 2n = 25 to 28) and was rich in pseudo-acrocentric chromosomes. We propose a hypothesis explaining the dynamics of the modifications which occurred in the karyotype of this species, based on the minimum interaction theory of Imai et al. (1986, 1988, 1994) and on the chromosome rearrangements and heteromorphisms observed by us.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-banding; Trypoxylon nitidum; Karyotype evolution.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300015

Comet assay comparison of different Corbicula fluminea (Mollusca) tissues for the detection of genotoxicity
Provedor de dados: Genetics and Molecular Biology
Autores: Rigonato,Janaina; Mantovani,Mário Sérgio; Jordão,Berenice Quinzani.
The comet assay was used to study the sensitivity of the widely distributed freshwater bivalve mollusk Corbicula fluminea to the DNA-damaging alkylating-agent methylmethane sulfonate (MMS). This study was undertaken to ascertain if C. fluminea is a good bioindicator of pollutants in aquatic environments and identify which C. fluminea tissue is most effective and practical for genotoxicity studies. The mollusks were exposed to 0.6, 1.2 or 2.4 X 10-4 M MMS for 40 min and their hemolymph, gill tissue and digestive gland tissue assessed for the level of DNA damage and the time needed for the tissues to recovery. Regression analysis showed a direct linear dose-response relationship between MMS concentration and the number of damaged cells for hemolymph and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corbicula fluminea; Hemolymph; Gills; Digestive gland; Comet assay; Biomonitoring.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300023

Cloning, mapping and molecular characterization of porcine progesterone receptor membrane component 2 (PGRMC2) gene
Provedor de dados: Genetics and Molecular Biology
Autores: Chen,Congying; Sargent,Carole; Quilter,Claire; Yang,Zhuqing; Ren,Jun; Affara,Nabeel; Brenig,Bertram; Huang,Lusheng.
Progesterone plays an important role in sow reproduction by stimulating classic genomic pathways via nuclear receptors and non-genomic pathways via membrane receptors such a progesterone receptor membrane component 2 (PGRMC2). In this work, we used radiation hybrid mapping to assign PGRMC2 to pig chromosome 8 and observed that this receptor has two transcripts in pigs. The full-length cDNA of the large transcript is 1858 bp long and contains a 669-bp open reading frame (ORF) encoding a protein of 223 amino acids. The shorter transcript encodes a protein of 170 amino acids. The porcine PGRMC2 gene consists of three exons 446 bp, 156 bp and 1259 bp in length. The promoter sequence is GC-rich and lacks a typical TATA box. Several putative cis-regulatory DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Expression profile; Molecular characterization; Physical mapping.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300015

Differential expression of the KLK2 and KLK3 genes in peripheral blood and tissues of patients with prostate cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Meola,Juliana; Goulart,Luiz R.; Oliveira,Jaqueline D.D.; Neves,Adriana F.; Oliveira Jr.,Waldesse P.; Saraiva,Ana C.M.; Capaneli,Andréia C.; Cardoso,Alexandra M.; Prado,Lindolfo D.; Borba,Sebastião A.; Silva,Heyder D.
We used the multiplex semi-quantitative reverse-transcriptase PCR (RT-PCR) to investigate kallikrein 2 and 3 (KLK2 and KLK3) mRNA levels in prostate tissue from 42 prostate cancer patients, 33 of whom were also assessed for peripheral blood KLK2 expression by qualitative semi-nested RT-PCR. We found that KLK2 was an important tissue biomarker for distinguishing between prostate cancer patients and those with benign prostatic hyperplasia, particularly when KLK2 expression was &gt; 60% of that of the beta2-microglobulin constitutive gene. Patients with an average relative expression value &gt; 0.6 (cutoff value) had an eleven-fold higher chance of having prostate cancer. When one or two tissues samples were evaluated for KLK2 expression using the cutoff...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kallikrein II; Molecular markers; Prostate cancer; PSA; Semi-quantitative RT-PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200001

Chromosomal variation, macroevolution and possible parapatric speciation in Mepraia spinolai (Porter) (Hemiptera: Reduviidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Frias,Daniel; Atria,Juan.
Mepraia spinolai is an endemic species in Chile that lives in wild and domestic habitats. It is the only species of the Reduviidae family that shows alate polymorphism; females are always wingless, but males can be found with and without wings. The M. spinolai karyotype consists of 10 pairs of autosomes and a complex sex determination system. Males from the northernmost regions I and II (latitude 18°-26° South) are always winged (braquipterous) and are X1X2Y, with a large Y chromosome. From region III to the metropolitan region (latitude 26°-33° South), males may be either winged or wingless but appear to be polymorphic for a small neo-Y chromosome, which may have originated by fracture of the large holocentric Y chromosome found in populations from...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200002

Comparative analyses of three swallow species (Aves, Passeriformes, Hirundinidae): Insights on karyotype evolution and genomic organization
Provedor de dados: Genetics and Molecular Biology
Autores: Barcellos,Suziane Alves; Kretschmer,Rafael; Souza,Marcelo Santos de; Costa,Alice Lemos; Degrandi,Tiago Marafiga; Lopes,Cassiane Furlan; Ferguson-Smith,Malcolm A.; Pereira,Jorge; Oliveira,Edivaldo Herculano Correa de; Gunski,Ricardo José; Garnero,Analía del Valle.
Abstract Despite the richness of species in the Hirudinidae family, little is known about the genome organization of swallows. The Progne tapera species presents genetic and morphological difference when compared to other members of the same genus. Hence, the aims of this study were to analyze the chromosomal evolution of three species Progne tapera, Progne chalybea and Pygochelidon cyanoleuca - by comparative chromosome painting using two sets of probes, Gallus gallus and Zenaida auriculata, in order to determine chromosome homologies and the relationship between these species. All karyotypes exhibited 76 chromosomes with similar morphology, except for the 5th, 6th and 7th chromosome pairs in P. cyanoleuca. Additionally, comparative chromosome painting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homology; Molecular cytogenetics; Fluorescent in situ hybridization; Hirundinidae.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100201

The karyotype of Nothoscordum arenarium Herter (Gilliesioideae, Alliaceae): a populational and cytomolecular analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Luiz G.R.; Crosa,Orfeo; Winge,Helga; Guerra,Marcelo.
The genus Nothoscordum Kunth comprises approximately 20 species native to South America. Karyologically, the genus is remarkable for its large chromosomes and Robertsonian translocations. Variation in chromosome number has been recorded in a few polyploid species and it is unknown among diploids. This study presents the chromosome number and morphology of 53 individuals of seven populations of N. arenarium Herter (2n = 10). In addition, karyotype analyses after C-banding, staining with CMA and DAPI, and in situ hybridization with 5S and 45S rDNA probes were performed in six individuals from one population. All individuals exhibited 2n = 10 (6M + 4A), except for one tetraploid (2n = 20, 12M + 8A) and one triploid (2n = 15, 9M + 6A) plant. C-banding revealed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CMA/DAPI staining; 5S and 45S rDNA; Heterochromatin; Nothoscordum; Robertsonian translocation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100016

Fibroblasts of skin fragments as a tool for the investigation of genetic diseases: technical recommendations
Provedor de dados: Genetics and Molecular Biology
Autores: Coelho,Janice Carneiro; Giugliani,Roberto.
Skin biopsies are frequently indicated for investigation and/or confirmation of genetic disorders. Although relatively simple and noninvasive, these procedures require care in order to increase probability of success and to avoid patient discomfort and unnecessary repeated analyses and associated laboratory fees. The present report highlights the importance of skin biopsies in genetic disorder diagnosis and presents general rules for collecting, storing, transporting and processing samples. We recommend its reading to professionals intending to use this important and sometimes fundamental diagnostic tool.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200004

Cardanol: toxicogenetic assessment and its effects when combined with cyclophosphamide
Provedor de dados: Genetics and Molecular Biology
Autores: Schneider,Beatriz Ursinos Catelan; Meza,Alisson; Beatriz,Adilson; Pesarini,João Renato; Carvalho,Pamela Castilho de; Mauro,Mariana de Oliveira; Karaziack,Caroline Bilhar; Cunha-Laura,Andréa Luiza; Monreal,Antônio Carlos Duenhas; Matuo,Renata; Lima,Dênis Pires de; Oliveira,Rodrigo Juliano.
Abstract Cardanol is an effective antioxidant and is a compound with antimutagenic and antitumoral activity. Here, we evaluated the genotoxic and mutagenic potential of saturated side chain cardanol and its effects in combination with cyclophosphamide in preventing DNA damage, apoptosis, and immunomodulation. Swiss mice were treated with cardanol (2.5, 5 and 10 mg/kg) alone or in combination with cyclophosphamide (100 mg/kg). The results showed that cardanol is an effective chemopreventive compound, with damage reduction percentages that ranged from 18.9 to 31.76% in the comet assay and from 45 to 97% in the micronucleus assay. Moreover, cardanol has the ability to reduce the frequency of apoptosis induced by cyclophosphamide. The compound did not show...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenolic lipid; Antimutagenesis; Micronucleus; Comet assay; Apoptosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200279

Genetic differences between Chibcha and Non-Chibcha speaking tribes based on mitochondrial DNA (mtDNA) haplogroups from 21 Amerindian tribes from Colombia
Provedor de dados: Genetics and Molecular Biology
Autores: Usme-Romero,Solangy; Alonso,Milena; Hernandez-Cuervo,Helena; Yunis,Emilio J.; Yunis,Juan J.
We analyzed the frequency of four mitochondrial DNA haplogroups in 424 individuals from 21 Colombian Amerindian tribes. Our results showed a high degree of mtDNA diversity and genetic heterogeneity. Frequencies of mtDNA haplogroups A and C were high in the majority of populations studied. The distribution of these four mtDNA haplogroups from Amerindian populations was different in the northern region of the country compared to those in the south. Haplogroup A was more frequently found among Amerindian tribes in northern Colombia, while haplogroup D was more frequent among tribes in the south. Haplogroups A, C and D have clinal tendencies in Colombia and South America in general. Populations belonging to the Chibcha linguistic family of Colombia and other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial DNA; Amerindian; Colombia; Chibcha; Genetic relationships.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200003

Genetic variants and haplotypes of the UGT1A9, 1A7 and 1A1 genes in Chinese Han
Provedor de dados: Genetics and Molecular Biology
Autores: Zhang,Xiaoqing; Ao,Guokun; Wang,Yuewen; Yan,Wei; Wang,Min; Chen,Erfei; Yang,Fangfang; Yang,Jin.
In this report, we describe combined polymorphisms of the UGT1A9, UGT1A7 and UGT1A1 genes in 100 unrelated, healthy Chinese Han subjects. The functional regions of these genes were sequenced and comprehensively analyzed for genetic polymorphisms. Thirty variants were detected, including five novel forms. Tentative functional predictions indicated that a Cys → Arg substitution at position 277 in the UGT1A7 gene could alter the protein conformation and that 12460T &gt; G in the 3'UTR might influence protein translation through specifically expressed miRNAs. UGT1A9*1b was a major functional variant in the subjects examined whereas the *1f allele had a frequency of only 0.5%. A special functional haplotype (GAGAAC) was identified for UGT1A9, 1A7 and 1A1. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chinese Han; Genetic polymorphisms; Haplotype; UGT1A.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300008

Contributions of weather variables for specific adaptation of rubber tree (Hevea brasiliensis Muell.- Arg) clones
Provedor de dados: Genetics and Molecular Biology
Autores: Priyadarshan,P.M.
The specific adaptation of 15 rubber tree (Hevea brasiliensis) clones was assessed by analyzing yield during a normal year (1997-98) and a year (1998-99) in which the yield was exceptional. Differences in yield in response to changes in weather conditions over the years were evident with clones RRII 203, RRIM 703, PB 5/51 and PB 235 which all exhibited a negative trend with increasing wind velocity during 1997-98, these clones also exhibited a negative correlation with minimum temperature during 1998-99. The prominent yield differences across the years made selection based on both yield and stability inevitable through computing weather variables and environmental index as covariant. To determine the contribution of variable(s) to genotype-environment (GE)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rubber clones; Weather variables; Specific adaptation; Hevea; Stability; Environmental index.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400006

Effects of double-stranded RNA on virulence of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) against the silverleaf whitefly, Bemisia tabaci strain B (Homoptera: Aleyrodidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Azevedo,Andréia Cristiane Souza; Sosa-Gómez,Daniel Ricardo; Faria,Marcos Rodrigues; Fungaro,Maria Helena Pelegrinelli.
Bands of double-stranded RNA (dsRNA) were detected in three out of twelve isolates of Paecilomyces fumosoroseus. Identity of these bands was confirmed by RNAse, DNAse and S1 nuclease treatments. The cure of dsRNA for one isolate (P92) was successfully carried out for a single conidium subculture. Isogenic strains, with or without dsRNA, were submitted to virulence tests against the whitefly Bemisia tabaci strain B. In contrast to findings for some phytopathogenic fungi, these dsRNA fragments did not cause hypovirulence in P. fumosoroseus.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100010

Genetic diversity in a Brazilian bovine herd based on four microsatellite loci
Provedor de dados: Genetics and Molecular Biology
Autores: Almeida,Sabrina E. Matos; Machado,Márcia S.N.; Steigleder,Clara Sabina; Gama,Cleonice L.; Hutz,Mara Helena; Henkes,Luiz Ernani; Moraes,José Carlos F.; Weimer,Tania de Azevedo.
Microsatellites or short tandem repeats (STRs), DNA markers relatively abundant in the genome, have a high degree of polymorphism and therefore great potential for characterizing populations. The present study estimates genetic variability in a set of four microsatellites (BMS3013, BMS3004, HEL10 and TGLA122) in a Brazilian hybrid bovine breed (5/8 Aberdeen Angus x 3/8 Nelore). The objectives were to determine the effect of crossbreeding and selection in these animals' genetic diversity as well as to discover the herd's genetic relationship with that of other breeds. Low diversity was verified in BMS3013 and high diversity was detected in BMS3004, HEL10 and TGLA122. Two alleles in TGLA122 are described here for the first time (TGLA122*155 and TGLA122*163)....
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200018

Mapping 18S ribosomal genes in fish of the genus Brycon (Characidae) by fluorescence in situ hybridization (FISH)
Provedor de dados: Genetics and Molecular Biology
Autores: Wasko,Adriane Pinto; Galetti Jr.,Pedro Manoel.
The present study provides data on the nucleolar organizer regions (NORs) of seven Brycon species based on mapping of the 18S rRNA genes by fluorescence in situ hybridization (FISH). Fluorescent signals were observed on the telomere of the long arm of two large submetacentric chromosomes, thus confirming the number and location of NORs previously revealed by other classical cytogenetic techniques. Although there were no inter- or intra-individual variations in the number and location of the 18S loci, NOR size polymorphism was observed between homologous chromosomes. The clustering and conservation of NORs in a single chromosome pair indicates a high level of NOR stability among species of the genus Brycon.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100025

Chromosomal characterization of cultured populations of Chilean coho salmon (Oncorhynchus kistuch)
Provedor de dados: Genetics and Molecular Biology
Autores: Colihueque V.,Nelson.
Chromosomal characterization of coho salmon samples from three fish farms in southern Chile (Polcura, Castro and Coyhaique) was carried out in order to compare their chromosome constitutions. All populations had a 2n = 60; however, Polcura and Coyhaique had a different chromosome arm number (NF = 110; 40m + 10sm + 10st/t) than Castro (NF = 108; 40m + 8sm + 12st/t). Variation in NF was due to chromosome pair 25, which was submetacentric in Coyhaique and Polcura, but subtelocentric in Castro. In all karyotypes, a large submetacentric chromosome pair exhibited an interstitial secondary constriction in the short arm. The observed variability in chromosome arm number agrees with previous reports for O. kisutch, and in this particular case it seemed to be caused...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100008

Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
Provedor de dados: Genetics and Molecular Biology
Autores: Naskar,Soumen; Deb,Sitangsu M.; Niranjan,Saket K.; Kumar,Subodh; Sharma,Deepak; Sakaram,Durgam; Sharma,Arjava.
In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β1 domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water buffalo; CDNA; DRB; MHC; Phylogeny.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100013

Genomic variation and population structure detected by single nucleotide polymorphism arrays in Corriedale, Merino and Creole sheep
Provedor de dados: Genetics and Molecular Biology
Autores: Grasso,Andrés N; Goldberg,Virginia; Navajas,Elly A; Iriarte,Wanda; Gimeno,Diego; Aguilar,Ignacio; Medrano,Juan F; Rincón,Gonzalo; Ciappesoni,Gabriel.
The aim of this study was to investigate the genetic diversity within and among three breeds of sheep: Corriedale, Merino and Creole. Sheep from the three breeds (Merino n = 110, Corriedale n = 108 and Creole n = 10) were genotyped using the Illumina Ovine SNP50 beadchip®. Genetic diversity was evaluated by comparing the minor allele frequency (MAF) among breeds. Population structure and genetic differentiation were assessed using STRUCTURE software, principal component analysis (PCA) and fixation index (F ST). Fixed markers (MAF = 0) that were different among breeds were identified as specific breed markers. Using a subset of 18,181 single nucleotide polymorphisms (SNPs), PCA and STUCTURE analysis were able to explain population stratification within...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allele frequencies breed composition; Fixation index; Ovine SNP50; Population stratification; Principal component analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300011

Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa
Provedor de dados: Genetics and Molecular Biology
Autores: Ceita,Geruza de Oliveira; Vilas-Boas,Laurival Antônio; Castilho,Marcelo Santos; Carazzolle,Marcelo Falsarella; Pirovani,Carlos Priminho; Selbach-Schnadelbach,Alessandra; Gramacho,Karina Peres; Ramos,Pablo Ivan Pereira; Barbosa,Luciana Veiga; Pereira,Gonçalo Amarante Guimarães; Góes-Neto,Aristóteles.
The phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime &amp;amp; Philips-Mora, causal agent of witches' broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. perniciosa was studied and the lanosterol 14α-demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed. ERG11 genomic DNA and cDNA were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Basidiomycota; Fungus; Ergosterol; Theobroma cacao.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500011

Non-mosaic partial duplication 12p in a patient with dysmorphic characteristics and developmental delay
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Jakeline Santos; Joaquim,Tatiana Mozer; Silva,Rosana Aparecida Bicudo da; Souza,Deise Helena de; Martelli,Lúcia Regina; Moretti-Ferreira,Danilo.
Abstract Duplication of the short arm of chromosome 12 is a rare chromosomal abnormality that may arise de novo or result from malsegregation of a balanced parental translocation. This study comprises the clinical description, cytogenetic and cytogenomic analyses and genotype-phenotype correlation in a patient with facial dysmorphism, developmental delay and intellectual impairment caused by non-mosaic partial duplication and a paracentric inversion 12p. The patient’s GTG-banded karyotype was 46,XX,invdup(12)(pter → p13.32::p11.1 → p13.31::p13.31 → qter). A genetic gain of approximately 28 Mb was detected in the chromosomal region arr[GRCh37]12p13.31-p11.1(6914072_34756209)x3. The chromosomal alteration seen in our patient is described as “pure” partial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Duplication 12p; Array-CGH; Facial dysmorphism.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100103

Association between genetic distances in wheat (Triticum aestivum L.) as estimated by AFLP and morphological markers
Provedor de dados: Genetics and Molecular Biology
Autores: Vieira,Eduardo Alano; Carvalho,Fernando Irajá Félix de; Bertan,Ivandro; Kopp,Mauricio Marini; Zimmer,Paulo Dejalma; Benin,Giovani; Silva,José Antônio Gonzalez da; Hartwig,Irineu; Malone,Gaspar; Oliveira,Antônio Costa de.
Molecular and morphological data analyses matrices are very informative tools for the estimation of genetic distances. We used AFLP markers, morphological traits and combined analysis to estimate the genetic distances between wheat genotypes and ascertain any associations between the two techneques. Nineteen wheat (Triticum aestivum L.) genotypes were analyzed using amplified fragment length polymorphism (AFLP) markers and field evaluated for two years. The matrices obtained by morphological and molecular marker data analyses revealed a significant but moderate correlation (r = 0.47), indicating that such techniques sample distinct genome regions. The combined analysis was found to be highly correlated with AFLP (r = 0.97) and moderately correlated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reeding; Genetic divergence; Molecular markers; Morphological traits.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300016

Microsatellite polymorphism in wheat from Brazilian cultivars; inter- and intra-varietal studies
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Vitor Lopes de Abreu; Seki,Homiko Abreu; Rumjanek,Franklin David.
Eleven samples of wheat (Triticum aestivum) from different Brazilian cultivars and six American varieties were compared for polymorphism, using primers for nine different STR loci. STR analysis of DNA from single grains of the Brazilian varieties showed that for most loci there was very little intra-cultivar polymorphism. The polymorphic variation observed for Brazilian cultivars was similar to that seen in the American varieties. For the Brazilian cultivars PCR analysis could be performed on only one half of a grain. The American samples required more seeds for analysis. The nucleotide sequences of five amplified microsatellites selected at random from the Brazilian samples were also determined and compared to those of the Chinese Spring variety. Although...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; Brazilian; Cultivars; DNA; STR; Polymorphism.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300021

Phytophthora parasitica transcriptome, a new concept in the understanding of the citrus gummosis
Provedor de dados: Genetics and Molecular Biology
Autores: Rosa,Daniel D.; Campos,Magnólia A.; Targon,Maria Luisa P.N.; Souza,Alessandra A.
Due to the economic importance of gummosis disease for the citriculture, studies on P. parasitica-Citrus interaction comprise a significant part in the Brazilian Citrus genome data bank (CitEST). Among them, two cDNA libraries constructed from two different growth conditions of the P. parasitica pathogen are included which has generated the PP/CitEST database (CitEST - Center APTA Citros Sylvio Moreira/IAC- Millennium Institute). Through this genomic approach and clustering analyses the following has been observed: out of a total of 13,285 available in the Phytophthora parasitica database, a group of 4,567 clusters was formed, comprising 2,649 singlets and 1,918 contigs. Out of a total of 4,567 possible genes, only 2,651 clusters were categorized; among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus disease; Elicitins; Plant pathogen; Gene expression profiles.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500028

Maternal drinking behavior and Fetal Alcohol Spectrum Disorders in adolescents with criminal behavior in southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Momino,Wakana; Félix,Têmis Maria; Abeche,Alberto Mantovani; Zandoná,Denise Isabel; Scheibler,Gabriela Gayer; Chambers,Christina; Jones,Kenneth Lyons; Flores,Renato Zamora; Schüler-Faccini,Lavínia.
Prenatal alcohol exposure can have serious and permanent adverse effects. The developing brain is the most vulnerable organ to the insults of prenatal alcohol exposure. A behavioral phenotype of prenatal alcohol exposure including conduct disorders is also described. This study on a sample of Brazilian adolescents convicted for criminal behavior aimed to evaluate possible clinical features of Fetal Alcohol Syndrome (FAS). These were compared to a control group of school adolescents, as well as tested for other environmental risk factors for antisocial behavior. A sample of 262 institutionalized male adolescents due to criminal behavior and 154 male students aged between 13 and 21 years comprised the study population. Maternal use of alcohol was admitted by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prenatal alcohol exposure; Fetal alcohol spectrum disorder; Criminal behavior; Conduct disorder; FAS; FASD.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600011

Genetic divergence between populations of the stingless bee uruçu amarela (Melipona rufiventris group, Hymenoptera, Meliponini): is there a new Melipona species in the Brazilian state of Minas Gerais?
Provedor de dados: Genetics and Molecular Biology
Autores: Tavares,Mara Garcia; Dias,Luiz Antonio dos Santos; Borges,Andréia Arantes; Lopes,Denilce Meneses; Busse,Angélica Helene Pereira; Costa,Ronaldo Guimarães; Salomão,Tânia Maria Fernandes; Campos,Lucio Antonio de Oliveira.
Allozyme, microsatellite and random amplified polymorphic DNA (RAPD) molecular markers were used to investigate the within and between population genetic variability and between population genetic differentiation of the Brazilian stingless bee uruçu amarela (nominally Melipona rufiventris Lepeletier, 1836) present in savanna and Atlantic forest habitats of the Brazilian state of Minas Gerais (MG). We found low levels of within population variability, although there were a large number of private alleles that specifically characterized these populations. The F ST values indicated a high level of genetic diversity between populations. Analysis of molecular variance (AMOVA) showed a high degree of population differentiation between the savanna and Atlantic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endangered species; Genetic divergence; Molecular markers; Stingless bees; Uruçu amarela.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400027

Effect of lactation length adjustment procedures on genetic parameter estimates for buffalo milk yield
Provedor de dados: Genetics and Molecular Biology
Autores: Baldi,Fernando; Laureano,Monyka Marianna Massolini; Gordo,Daniel Gustavo Mansan; Bignardi,Annaiza Braga; Borquis,Rusbel Raul Aspilcueta; Albuquerque,Lucia Galvão de; Tonhati,Humberto.
The objectives of this study were to estimate the genetic parameters for milk yield unadjusted and adjusted for days in milk and, subsequently, to assess the influence of adjusting for days in milk on sire rank. Complete lactations from 90 or 150 days of lactation to 270 or 350 days in milk were considered in these analyses. Milk yield was adjusted for days in milk by multiplicative correction factors, or by including lactation length as a covariable in the model. Milk yields adjusted by different procedures were considered as different traits. Heritability estimates varied from 0.17 to 0.28. Genetic correlation estimates between milk yields unadjusted and adjusted for days in milk were greater than 0.82. Adjusting for days in milk affected the parameter...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic correlations; Heritability; Lactation length; Milk production; Rank correlations.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100012

Survey of glycine-rich proteins (GRPs) in the Eucalyptus expressed sequence tag database (ForEST)
Provedor de dados: Genetics and Molecular Biology
Autores: Bocca,Silvia Nora; Magioli,Claudia; Mangeon,Amanda; Junqueira,Ricardo Magrani; Cardeal,Vanessa; Margis,Rogério; Sachetto-Martins,Gilberto.
The occurrence of quasi-repetitive glycine-rich peptides has been reported in different organisms. Glycine-rich regions are proposed to be involved in protein-protein interactions in some mammalian protein families. In plants, a set of glycine-rich proteins (GRPs) was characterized several years ago, and since then a wealth of new GRPs have been identified. GRPs may have very diverse sub-cellular localization and functions. The only common feature among all different GRPs is the presence of glycine-rich repeat domains. The expression of genes encoding GRPs is developmentally regulated, and also induced, in several plant genera, by physical, chemical and biological factors. In addition to the highly modulated expression, several GRPs also show...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine-rich; GRP; Eucalyptus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400016

Ecological drivers of plant genetic diversity at the southern edge of geographical distributions: Forestal vines in a temperate region
Provedor de dados: Genetics and Molecular Biology
Autores: Barros,Michel J.F.; Diniz-Filho,José Alexandre F.; Freitas,Loreta B.
Abstract The Tropical Niche Conservatism hypothesis is one of the most relevant theories to explain why tropical diversity is high, although the mechanisms underlying this hypothesis require further clarification. A possible research avenue to address the underlying mechanisms includes determining population-level processes associated with such a hypothesis, in particular by trying to identify how adaptation may occur in extreme niche conditions at the edges of species ranges. However, the determinants of molecular diversity at the edges of geographical distributions of tropical taxa are still poorly known. Here we assessed which environmental variables determine diversity in nuclear and plastid genetic markers for populations of four Passiflora species in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Distribution edges; Genetic diversity; Passiflora; Niche conservatism; Environmental drivers.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200318

Expression in normals and in subjects with schizophrenia of a novel gene fragment originally isolated from monozygotic twins discordant for schizophrenia
Provedor de dados: Genetics and Molecular Biology
Autores: Zumárraga,Mercedes; Andía,Isabel; Dávila,Ricardo; Miller,Jeannette C.; Friedhoff,Arnold J.
Differentially expressed clones from subtracted cDNA libraries of a pair of monozygotic twins discordant for schizophrenia have been reported in the literature. The clones were expressed in lymphocytes from the healthy twin, but not from the schizophrenic twin. In the current study, we assessed the expression of one of these clones, oksc12b, in 10 normal controls and in 10 patients who met DSM-IV criteria for schizophrenia and had never received neuroleptic medication. We hypothesized that this clone would be differentially expressed in normal controls and in the schizophrenic patients, and that its expression could be a peripheral marker of the disease. Lymphocytes were isolated and total RNA was purified, reverse-transcribed, and quantified by two PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monozygotic twins; MRNA; Schizophrenia.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100004

Knockdown of E2f1 by RNA interference impairs proliferation of rat cells in vitro
Provedor de dados: Genetics and Molecular Biology
Autores: Vasques,Luciana dos Reis; Pujiz,Regiane Simoni; Strauss,Bryan Eric; Krieger,José Eduardo.
E2F1 plays a key role in cell-cycle regulation in mammals, since its transcription factor activity controls genes required for DNA synthesis and apoptosis. E2F1 deregulation is a common feature among different tumor types and can be a major cause of cell proliferation. Thus, blocking E2F1 expression by RNA interference represents a promising therapeutic approach. In this study, the introduction of specific short hairpin RNAs (shRNAs) reduced E2f1 expression by up to 77%, and impaired rat glioma cell proliferation by approximately 70%, as compared to control cells. Furthermore, we investigated the expression of E2f1 target genes, Cyclin A and Cyclin E. Cyclin A was found to be down-regulated, whereas Cyclin E had similar expression to control cells,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNAi; ShRNA; E2F1; Proliferation; Cancer.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100005

Persistent double strand break accumulation does not precede cell death in an Olaparib-sensitive BRCA-deficient colorectal cancer cell model
Provedor de dados: Genetics and Molecular Biology
Autores: Paviolo,Natalia Soledad; Vega,María Belén de la; Pansa,María Florencia; García,Iris Alejandra; Calzetta,Nicolás Luis; Soria,Gastón; Gottifredi,Vanesa.
Abstract The poly (adenosine diphosphate (ADP)-ribosyl) polymerase inhibitors (PARPi) selectively kill cancer cells with BRCA1 or BRCA2 (BRCA)-mutations. It has been proposed that cell death induction after PARPi depends on unrepaired double strand breaks (DSBs) that accumulate due to the homologous recombination deficiency of BRCA-mutated cells. Such accumulation of DSBs is inferred mainly from the high levels of DNA damage markers like phosphorylated histone H2AX. Herein, we developed a model of isogenic cell lines to show that depletion of BRCA causes PARPi-triggered cell death, replication stress (phosphorylated-H2AX and 53BP1 foci), and genomic instability. However, persistent DSBs accumulation was not detected under the same experimental conditions....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GammaH2AX; Alternative end joining; Non-homologous end joining; Homologous recombination; PARP.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200303

Genetic structure of red-handed howler monkey populations in the fragmented landscape of Eastern Brazilian Amazonia
Provedor de dados: Genetics and Molecular Biology
Autores: Bastos,Heitor B.; Gonçalves,Evonnildo C.; Ferrari,Stephen F.; Silva,Artur; Schneider,Maria Paula C.
We genotyped 15 microsatellite loci in order to evaluate the effects of habitat fragmentation, caused by flooding of the Tucuruí reservoir, on the genetic structure of Alouatta belzebul in eastern Amazonia. The analysis included two populations sampled in 1984, representing both margins of the Tocantins river, and three populations sampled 18 years later. Minimal differences in the diversity levels between present-day (Ho = 0.62-0.69 and A R = 6.07-7.21) and pre-flooding (Ho = 0.60-0.62 and A R = 6.27-6.77) populations indicated there was no significant loss of genetic variability, possibly because of successful management strategies applied during the flooding. The changes observed were limited to shifts in the composition of alleles, which presumably...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alouatta belzebul; Amazonia; Conservation; Genetic structure; Habitat fragmentation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400027

In silico analysis of ESTs from roots of Rangpur lime (Citrus limonia Osbeck) under water stress
Provedor de dados: Genetics and Molecular Biology
Autores: Boscariol-Camargo,Raquel L.; Berger,Irving J.; Souza,Alessandra A.; Amaral,Alexandre M. do; Carlos,Eduardo F.; Freitas-Astúa,Juliana; Takita,Marco A.; Targon,Maria Luisa P.N.; Medina,Camilo L.; Reis,Marcelo S.; Machado,Marcos A.
CitEST project resulted in the construction of cDNA libraries from different Citrus sp. tissues under various physiological conditions. Among them, plantlets of Rangpur lime were exposed to hydroponic conditions with and without water stress using PEG6000. RNA from roots was obtained and generated a total of 4,130 valid cDNA reads, with 2,020 from the non-stressed condition and 2,110 from the stressed set. Bioinformatic analyses measured the frequency of each read in the libraries and yielded an in silico transcriptional profile for each condition. A total of 40 contigs were differentially expressed and allowed to detect up-regulated homologue sequences to well known genes involved in stress response, such as aquaporins, dehydrin, sucrose synthase, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Expressed sequence tags; Transcription; Rootstock; Drought; Molecular response.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500019

The nucleation of microtubules in Aspergillus nidulans germlings
Provedor de dados: Genetics and Molecular Biology
Autores: Andrade-Monteiro,Cristina de; Martinez-Rossi,Nilce M.
Microtubules are filaments composed of dimers of alpha- and beta-tubulins, which have a variety of functions in living cells. In fungi, the spindle pole bodies usually have been considered to be microtubule-organizing centers. We used the antimicrotubule drug Benomyl in block/release experiments to depolymerize and repolymerize microtubules in Aspergillus nidulans germlings to learn more about the microtubule nucleation process in this filamentous fungus. Twenty seconds after release from Benomyl short microtubules were formed from several bright (immunofluorescent) dots distributed along the germlings, suggesting that microtubule nucleation is randomly distributed in A. nidulans germlings. Since nuclear movement is dependent on microtubules in A. nidulans...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300004

A microsatellite study of bovine solute carrier family 11 a1 (Slc11a1) gene diversity in Mexico in relation to bovine tuberculosis
Provedor de dados: Genetics and Molecular Biology
Autores: Vázquez-Flores,Felicitas; Alonso,Rogelio; Villegas-Sepúlveda,Nicolás; Arriaga,Camila; Pereira-Suárez,Ana Laura; Mancilla,Raúl; Estrada-Chávez,Ciro.
Bovine tuberculosis, caused by Mycobacterium bovis, is a disease of socio-economic and public health importance and of significance to international trade regulation. Allelic variants of several genes have been implicated in the genetic susceptibility to tuberculosis in some human populations, but little is known in cattle. We surveyed 34 European, 18 Asian, 20 Creole and 23 hybrid bovines for polymorphisms of the bovine solute carrier family 11 a1(Slc11a1) gene, formerly known as natural resistance associated macrophage protein (Nramp1), gene by typing the cattle using two microsatellite loci closely linked to this gene. The microsatellites used were 311-22, located at the 3' untranslated region (3' UTR) of the Slc11a1 gene, and ARO28 situated about 0.6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine lineages; Mycobacterium bovis; Microsatellite loci; Nramp1; Slc11a1; 3' UTR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300019

Establishment of an adherent cell layer from human umbilical cord blood
Provedor de dados: Genetics and Molecular Biology
Autores: Alfonso,Zeni Z.C.; Forneck,Eduardo D.; Allebrandt,Waldir F.; Nardi,Nance B.
In addition to bone marrow and peripheral blood, stem cells also occur in human umbilical cord blood (HUCB), and there is an increasing interest in the use of this material as an alternative source for bone marrow transplantation and gene therapy. In vitro hematopoiesis has been maintained for up to 16 weeks in HUCB cultures, but the establishment of an adherent, stromal layer has consistently failed. Adherent cell precursors among mononuclear cells from HUCB were sought for in long-term cultures. Mononuclear cells obtained from cord blood after full term, normal deliveries were cultivated at different concentrations in Iscove's modified Dulbecco's medium (IMDM) with weekly feeding. An adherent layer was detected in 16 of 30 cultures, 12 of which were...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300002

James V. Neel and Latin America - or how scientific collaboration should be conducted
Provedor de dados: Genetics and Molecular Biology
Autores: Salzano,Francisco M.
The main events related to the life of James V. Neel, one of the most important geneticists of the last decades, are presented, especially with reference to the influence he exerted in Latin America. Documentation is provided on the field work he performed in Brazil, and on the results of the joint Ann Arbor-Porto Alegre program of research.
Tipo: Info:eu-repo/semantics/report
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300010

Novel compound heterozygous mutations of ALDH1A3 contribute to anophthalmia in a non-consanguineous Chinese family
Provedor de dados: Genetics and Molecular Biology
Autores: Liu,Yunqiang; Lu,Yongjie; Liu,Shasha; Liao,Shunyao.
Abstract Anophthalmia is a rare eye development anomaly resulting in absent ocular globes or tissue in the orbit since birth. Here, we investigated a newborn with bilateral anophthalmia in a Chinese family. Exome sequencing revealed that compound heterozygous mutations c.287G &gt; A (p.(Arg96His)) and c.709G &gt; A (p.(Gly237Arg)) of the ALDH1A3 gene were present in the affected newborn. Both mutations were absent in all of the searched databases, including 10,000 in-house Chinese exome sequences, and these mutations were confirmed as having been transmitted from the parents. Comparative amino acid sequence analysis across distantly related species revealed that the residues at positions 96 and 234 were evolutionarily highly conserved. In silico analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anophthalmia; Exome sequence; ALDH1A3; Compound heterozygous mutations.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300430

Cashew (Anacardium occidentale) apple juice lowers mutagenicity of aflatoxin B1 in S. typhimurium TA102
Provedor de dados: Genetics and Molecular Biology
Autores: Cavalcante,Ana Amélia Melo; Rübensam,Gabriel; Erdtmann,Bernardo; Brendel,Martin; Henriques,João A.P.
Cashew (Anacardium occidentale) is a medicinal plant native to Brazil and also yields a nutritious fruit juice. Its large pulpy pseudo-fruit, referred to as the cashew apple, contains high concentrations of vitamin C, carotenoids, phenolic compounds and minerals. Natural and processed cashew apple juice (CAJ/cajuina) are amongst the most popular juices in Brazil, especially in the north-east. Both juices have antioxidant potential and suppress mutagenicity of hydrogen peroxide. In the present study we evaluated the inhibitory effects of CAJ/cajuina on Aflatoxin B1(AFB1)-induced mutation, using the Salmonella/microsome assay with the experimental approaches of pre-, co- and post-treatments. Both CAJ/cajuina suppress AFB1-induced mutagenesis in strain TA102...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cashew apple juice; Cajuina; Anti-mutagenicity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200025

Detection of self-incompatible oilseed rape plants (Brassica napus L.) based on molecular markers for identification of the class I S haplotype
Provedor de dados: Genetics and Molecular Biology
Autores: Havlícková,Lenka; Jozová,Eva; Klíma,Miroslav; Kucera,Vratislav; Curn,Vladislav.
The selection of desirable genotypes with recessive characteristics, such as self-incompatible plants, is often difficult or even impossible and represents a crucial barrier in accelerating the breeding process. Molecular approaches and selection based on molecular markers can allow breeders to overcome this limitation. The use of self-incompatibility is an alternative in hybrid breeding of oilseed rape. Unfortunately, stable self-incompatibility is recessive and phenotype-based selection is very difficult and time-consuming. The development of reliable molecular markers for detecting desirable plants with functional self-incompatible genes is of great importance for breeders and allows selection at early stages of plant growth. Because most of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Double haploid; Hybrid breeding; MAS; S locus; SLG.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400012

Say syndrome: A new Brazilian case
Provedor de dados: Genetics and Molecular Biology
Autores: Guion-Almeida,M.L.; Kokitsu-Nakata,N.M.; Zechi,R.M.
We report on a Brazilian boy, born to nonconsanguineous parents, who presented short stature, microcephaly, large ears, Robin sequence, hand anomalies, delayed bone age, and developmental delay. Major signs found in this patient are related to the Say syndrome.
Tipo: Info:eu-repo/semantics/other
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400007

A second case of multivalent meiotic configurations in diploid species of Anura
Provedor de dados: Genetics and Molecular Biology
Autores: Lourenço,Luciana Bolsoni; Recco-Pimentel,Shirlei M.; Cardoso,Adão José.
We analyzed the meiotic chromosomes of specimens from the two karyological groups of Physalaemus petersi (Jiménez de la Espada, 1872) described in the literature. Multivalent configurations (rings or chains) were observed in both groups. This meiotic organization resulted from the terminal association of non-homologous chromosomes, and an analysis of C-banded multivalents indicated no involvement of heterochromatic regions in these associations. A possible explanation for such meiotic configurations is the occurrence of heterozygous translocations. Thus, multivalents may indicate the involvement of translocation events in the karyotypic evolution of P. petersi.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100024

CDH1 mutations in gastric cancer patients from northern Brazil identified by Next- Generation Sequencing (NGS)
Provedor de dados: Genetics and Molecular Biology
Autores: El-Husny,Antonette; Raiol-Moraes,Milene; Amador,Marcos; Ribeiro-dos-Santos,André M.; Montagnini,André; Barbosa,Silvanira; Silva,Artur; Assumpção,Paulo; Ishak,Geraldo; Santos,Sidney; Pinto,Pablo; Cruz,Aline; Ribeiro-dos-Santos,Ândrea.
Abstract Gastric cancer is considered to be the fifth highest incident tumor worldwide and the third leading cause of cancer deaths. Developing regions report a higher number of sporadic cases, but there are only a few local studies related to hereditary cases of gastric cancer in Brazil to confirm this fact. CDH1 germline mutations have been described both in familial and sporadic cases, but there is only one recent molecular description of individuals from Brazil. In this study we performed Next Generation Sequencing (NGS) to assess CDH1 germline mutations in individuals who match the clinical criteria for Hereditary Diffuse Gastric Cancer (HDGC), or who exhibit very early diagnosis of gastric cancer. Among five probands we detected CDH1 germline...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CDH1; Germline mutations; HDGC; Gastric Cancer; NGS.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200189

Inclusion of genetic relationship information in the pedigree selection method using mixed models
Provedor de dados: Genetics and Molecular Biology
Autores: Nunes,José Airton Rodrigues; Ramalho,Magno Antonio Patto; Ferreira,Daniel Furtado.
We used a mixed model approach and computer simulation to evaluate the inclusion of parentage information as determined by the genealogy established in the pedigree method. The simulations were based on a purely additive genetic model for one quantitative trait of 20 unlinked segregating loci with equal effects and an allelic frequency of 0.5 for heritability values of 10%, 25%, 50% and 75% for selection based on an F4:5 progeny mean. We simulated 1000 experiments for each heritability value, corresponding to the evaluation of 256 F4:5 progenies. The phenotypic values of the progenies were analyzed according to two models, one ignoring and one considering the additive genetic parentage among the progenies. The additive relationship coefficients among F4:5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autogamous crops; BLUP; Computer simulation; Plant breeding.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100015

Geleophysic dysplasia: Report on two sibs
Provedor de dados: Genetics and Molecular Biology
Autores: Boy,Raquel; Llerena,Juan; Pimentel,Márcia Mattos Gonçalves; Almeida,José Carlos Cabral de.
The authors describe two additional cases of Geleophysic dysplasia in siblings, which is a rare autosomal recessive disorder of glycoprotein metabolism whose basic defects remain to be determined.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100027

Factor XI deficiency in Indian Bos taurus, Bos indicus, Bos taurus x Bos indicus crossbreds and Bubalus bubalis
Provedor de dados: Genetics and Molecular Biology
Autores: Patel,Rajesh K.; Soni,Kalpesh J.; Chauhan,Jenabhai B.; Singh,Krishna M.; Sambasiva Rao,Krothapalli R.S.
We investigated the occurrence of Factor XI (FXI) deficiency syndrome in the following Indian dairy animals: Bos taurus Holstein-Friesian and Jersey cattle, Bos indicus Indian cattle breeds, B. taurus x B. indicus crossbreds and the river buffalo Bubalus bubalis. Factor XI deficiency is an autosomal recessive bleeding disorder known to affect Holstein cattle worldwide. A total of 1001 dairy animals, mainly bulls, were genotyped to detect the mutation within exon 12 of the gene encoding for factor XI. Two Holstein bulls were detected as heterozygous (carrier) for FXI deficiency, giving a carrier frequency of 0.6% in Indian Holstein cattle. None of the other cattle or buffalo breeds was found to be a carrier for FXI. Sequence comparison between normal and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Autosomal recessive disease; Blood coagulation; Buffaloes; Factor XI; Holstein-Friesian dairy cattle.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400013

Letter to the Editor regarding Hubacek et al.’s report “Lack of an association between SNPs within the cholinergic receptor genes and smoking behavior in a Czech post-MONICA study”
Provedor de dados: Genetics and Molecular Biology
Autores: Müderrisoglu,Ahmet; Babaoglu,Melih.
Tipo: Info:eu-repo/semantics/other
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300417

Chromosome studies in the genus Mikania (Asteraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Ruas,Paulo M.; Ruas,Claudete F.; Maffei,Eliane M.D.; Marin-Morales,Maria A.; Aguiar-Perecin,Margarida L.R.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400042

Karyotype description of two Neotropical Psittacidae species: the endangered Hyacinth Macaw, Anodorhynchus hyacinthinus, and the Hawk-headed Parrot, Deroptyus accipitrinus (Psittaciformes: Aves), and its significance for conservation plans
Provedor de dados: Genetics and Molecular Biology
Autores: Lunardi,Vitor de Oliveira; Francisco,Mercival Roberto; Rocha,Guaracy Tadeu; Goldschmidt,Beatriz; Galetti Junior,Pedro Manoel.
Neotropical parrots are among the most threatened groups of birds in the world, and many species are facing extinction in a near future. At the same time, the taxonomic position of many species remains unclear. Karyotype analysis has been used to elucidate the phylogenetic status of many bird groups, also providing important information for both in situ and ex situ conservation plans. The objective of the present study was to describe for the first time the karyotypes of the endangered Hyacinth Macaw, Anodorhynchus hyacinthinus, and of the Hawk-headed Parrot, Deroptyus accipitrinus. A diploid number of 2n = 70 and a karyotype similar to the main pattern previously found for the genera Ara, Cyanopsitta, Aratinga, Propyrrhura, Pionites, Pionopsitta,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian karyotype; Anodorhynchus; Deroptyus; Psittaciformes; Conservation biology; Cytotaxonomy.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300011

Polytene chromosomes and phylogenetic relationships in ten Drosophila species of the annulimana group (Diptera, Drosophilidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Tosi,Deborah; Pereira,Maria Augusta Q.R.; Vilela,Carlos R.
Polytene chromosomes banding patterns of ten of the 16 species of the Neotropical annulimana group of Drosophila were used to propose phylogenetic relationships among species. Drosophila annulimana chromosomes were used as the standard sequence and the most parsimonious series of changes (paracentric inversions) were considered. In some cases, intermediate hypothetical rearrangements were proposed to explain the sequences present in a given species. A total of 47 paracentric inversions were detected, most of them (44.7%) in chromosome 4. Three subgroups, partially coincident with those previously proposed based on morphological and karyotypical analyses, were classified as: 1) annulimana subgroup (Drosophila annulimana, D. aracataca, D. aragua, and D....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome phylogeny; Drosophilinae; Imagos; Neotropical Region; Paracentric inversions.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600023

Y-STR diversity and ethnic admixture in White and Mulatto Brazilian population samples
Provedor de dados: Genetics and Molecular Biology
Autores: Ferreira,Luzitano Brandão; Mendes-Junior,Celso Teixeira; Wiezel,Cláudia Emília Vieira; Luizon,Marcelo Rizzatti; Simões,Aguinaldo Luiz.
We investigated 50 Mulatto and 120 White Brazilians for the Y-chromosome short tandem repeat (Y-STR) markers (DYS19, DYS390, DYS391, DYS392 and DYS393) and found 79 different haplotypes in the White and 35 in the Mulatto sample. Admixture estimates based on allele frequencies showed that the admixture of the white sample was 89% European, 6% African and 5% Amerindian while the Mulatto sample was 93% European and 7% African. Results were consistent with historical records of the directional mating between European males and Amerindian or African females.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Mulatto; White; Y-STR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400004

Ernesto Paterniani (1928-2009): a life devoted to Genetics and Breeding
Provedor de dados: Genetics and Molecular Biology
Autores: Vencovsky,Roland; Bandel,Gerhard.
Tipo: Info:eu-repo/semantics/report
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300002

Comparative cytogenetics of Hoplias malabaricus (Pisces, Erythrinidae): a population analysis in adjacent hydrographic basins
Provedor de dados: Genetics and Molecular Biology
Autores: Vicari,Marcelo Ricardo; Artoni,Roberto Ferreira; Bertollo,Luiz Antonio Carlos.
The chromosomes of specimens from four Hoplias malabaricus populations from headwaters of adjacent river basins at Ponta Grossa, southern Brazil, were investigated using differential staining techniques (C-banding, AgNO3 and CMA3) and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. The diploid chromosome number in representatives of all four populations was invariably 2n = 42, with karyotypes composed of 12 pairs of metacentrics and 9 pairs of submetacentrics, without heteromorphic sex chromosomes. This kind of karyotype represents cytotype A in regard to cytotypes identified previously in H. malabaricus, exhibiting however, at the same time, some differences in the distribution of constitutive heterochromatin segments and in the locations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome banding; FISH; Cytotaxonomy of trahiras; Karyotype; NOR phenotype.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100018

Comparative cytogenetics of Carnegiella marthae and Carnegiella strigata (Characiformes, Gasteropelecidae) and description of a ZZ/ZW sex chromosome system
Provedor de dados: Genetics and Molecular Biology
Autores: Terencio,Maria Leandra; Schneider,Carlos Henrique; Gross,Maria Claudia; Silva,Adailton Moreira da; Feldberg,Eliana; Porto,Jorge Ivan Rebelo.
Comparative cytogenetic analyses of hatchetfishes Carnegiella marthae and Carnegiella strigata (Gasteropelecidae) from the Rio Negro basin were performed using conventional Giemsa staining, silver (Ag) -staining and C-banding. The diploid chromosome numbers of both species equaled 2n = 50 but their karyotypes were distinct. We found evidence for sex chromosomes in C. marthae since karyotype of males presented 20 M + 12 SM + 4 ST + 14 A and ZZ ST chromosomes while the females presented 20 M + 12 SM + 4 ST + 14 A and ZW ST chromosomes of distinct size. Conversely, C. strigata presented 4 M + 4 SM + 2 ST + 40 A chromosomes without sex chromosome heteromorphism. Karyotypes of both species had two NOR-bearing SM chromosomes of distinct size indicating the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish cytogenetics; Karyotype differentiation; NOR phenotypes; Heteromorphic sex chromosome system; Cytotaxonomy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200011

Caffeine inheritance in interspecific hybrids of Coffea arabica x Coffea canephora (Gentianales, Rubiaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Priolli,Regina H.G.; Mazzafera,Paulo; Siqueira,Walter J.; Möller,Milene; Zucchi,Maria Imaculada; Ramos,Luis Carlos S.; Gallo,Paulo B.; Colombo,Carlos A.
Caffeine inheritance was investigated in F2 and BC1F1 generations between Coffea arabica var. Bourbon Vermelho (BV) and Coffea canephora var. Robusta 4x (R4x). The caffeine content of seeds and leaves was determined during 2004 and 2005. Microsatellite loci-markers were used to deduce the meiotic pattern of chromosome pairing of tetraploid interspecific hybrids. Genetic analysis indicated that caffeine content in seeds was quantitatively inherited and controlled by genes with additive effects. The estimates of broad-sense heritability of caffeine content in seeds were high for both generations. In coffee leaves, the caffeine content (BSH) from the same populations showed transgressive segregants with enhanced levels and high BSH. Segregation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broad sense heritability; Coffee breeding; Microsatellite-markers; Quantitative genes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300017

Genetic effect on apgar score
Provedor de dados: Genetics and Molecular Biology
Autores: Franchi-Pinto,Carla; Colletto,Glória Maria Duccini Dal; Krieger,Henrique; Beiguelman,Bernardo.
Intraclass correlation coefficients for one- and five-min Apgar scores of 604 twin pairs born at a southeastern Brazilian hospital were calculated, after adjusting these scores for gestational age and sex. The data support a genetic hypothesis only for 1-min Apgar score, probably because it is less affected by the environment than 4 min later, after the newborns have been under the care of a neonatology team. First-born twins exhibited, on average, better clinical conditions than second-born twins. The former showed a significantly lower proportion of Apgar scores under seven than second-born twins, both at 1 min (17.5% vs. 29.8%) and at 5 min (7.2% vs. 11.9%). The proportion of children born with "good" Apgar scores was significantly smaller among twins...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100003

Transcriptome sequencing and expression profiling of genes involved in the response to abiotic stress in Medicago ruthenica
Provedor de dados: Genetics and Molecular Biology
Autores: Shu,Yongjun; Li,Wei; Zhao,Jinyue; Liu,Ying; Guo,Changhong.
Abstract Medicago ruthenica is a perennial forage legume with the remarkable ability to survive under unfavorable environmental conditions. It has been identified as an excellent species of Medicago that can adapt to various environmental stresses including low temperature, drought, and salinity. To investigate its potential as a genetic resource, we performed transcriptome sequencing and analysis in M. ruthenica under abiotic stresses. We generated &gt;120 million reads from six cDNA libraries, resulting in 79,249 unique transcripts, most of which were highly similar to transcripts from M. truncatula (44,608, 56.3%) and alfalfa (M. sativa, 48,023, 60.6%). Based on gene expression profiles, 2,721 transcripts were identified as abiotic stress responsive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicago ruthenica; RNA-Seq; Transcription factor (TF); Abiotic stress; Reactive oxygen species (ROS).
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400638

Genetic distance between broodstocks of the marine shrimp Litopenaeus vannamei (Decapoda, Penaeidae) by mtDNA analyses
Provedor de dados: Genetics and Molecular Biology
Autores: Francisco,Ana Karina de; Galetti Junior,Pedro Manoel.
In the constantly growing Brazilian shrimp industry the evaluation of genetic relationships between broodstocks is an useful tool for shrimp culture management programs. We established the genetic relationships between five broodstocks of the white marine shrimp Litopenaeus vannamei (Penaeidae) based on the sequencing of the mtDNA 16S rRNA and cytochrome oxidase I (COI) regions. Although no divergence was found between the broodstocks for the highly conservative 16S rRNA gene, we did find an 8.2% distance between L. vannamei and the Farfantepenaeus subtilis. Analyses of the COI region showed genetic distances of only 0.2 to 1% between the broodstocks, which contrasted with the 10.9% mean distance found between L. vannamei and F. subtilis. The small genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shrimp; Litopenaeus vannamei; Genetic variation; MtDNA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200014

Screening fetal losses for monosomy X with a simple PCR-based procedure
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Rinaldo W.; Sturzeneker,Rosane; Pena,Sérgio D.J.
To screen for monosomy X in spontaneous fetal losses we explored a simple molecular strategy based on loss of heterozygosity at highly polymorphic X-linked loci. We developed a multiplex fluorescent procedure that allows the simultaneous amplification of five dinucleotide repeat polymorphisms in a large low-recombination region in the long arm of the X chromosome. Analysis was performed by computer-assisted laser densitometry. We did not find any instances of homozygosity at all five loci in 30 normal females tested, nor among 37 women whose typing data were retrieved from the Fondation Jean Dausset - CEPH genotype database. In addition, all cases of monosomy X previously diagnosed by conventional cytogenetics presented the anticipated loss of...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100002

Early selection for growth vigor in rubber  tree genotypes in northwestern São  Paulo State (Brazil)
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Paulo de Souza; Bortoletto,Nelson; Fonseca,Fernando da Silva; Bataglia,Ondino Cleante; Ortolani,Altino Aldo.
Forty-five genotypes (clones) of rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Müell. Arg.] of different origins were assessed for efficiency of early selection at the Votuporanga Experimental Station in northwestern São Paulo State, Brazil, using a randomized complete block design with three replications and six plants per plot. Girth at 120 cm above the highest point of the grafting union of each tree was taken at ages 12, 24, 36, 48, 60, 72, 84 and 96 months. Highly significant differences in girth were detected among the genotypes at all ages evaluated, except for 12 months. Estimates of genetic variance for two age sets showed a substantial increase with age, while the genotype variation decreased. Selection made at 24 months proved to be...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400018

Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Xiang,Heng; Zhang,Ruizhi; Li,Nengzhang; Vossbrinck,Charles R.
There are numerous examples of convergent evolution in nature. Major ecological adaptations such as flight, loss of limbs in vertebrates, pesticide resistance, adaptation to a parasitic way of life, etc., have all evolved more than once, as seen by their analogous functions in separate taxa. But what about protein evolution? Does the environment have a strong enough influence on intracellular processes that enzymes and other functional proteins play, to evolve similar functional roles separately in different organisms? Manganese Superoxide Dismutase (MnSOD) is a manganesedependant metallo-enzyme which plays a crucial role in protecting cells from anti-oxidative stress by eliminating reactive (superoxide) oxygen species. It is a ubiquitous housekeeping...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Convergence; Hydrophobic cluster analysis; Manganese superoxide dismutase; Phylogeny; Structure.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300019

Micropropagation of rubber trees (Hevea brasiliensis Muell. Arg.)
Provedor de dados: Genetics and Molecular Biology
Autores: Mendanha,Asseara Batista Leitão; Torres,Roberto Augusto de Almeida; Freire,Adelson de Barros.
Tissue cultures were established from newly expanded leaves and axillary buds of rubber trees (Hevea brasiliensis Muell. Arg.). Calli formed from these explants, but no regeneration occurred. Shoots were obtained from axillary buds cultured on Murashige and Skoog's (MS) medium (Physiol. Plant. 15: 473-497, 1962) supplemented with 1.0 mg/l kinetin, 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 20 g/l sucrose and 4 g/l Difco agar. Formation of a root similar to a tap root was induced on MS medium supplemented with 5.0 mg/l naphthaleneacetic acid (NAA), 3.0 mg/l indolylbutyric acid (IBA), 50 g/l sucrose and 4 g/l Difco agar. Several types of explants were used in attempts to recover complete rubber tree plants with well-developed tap roots. Leaf explants...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300018

Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence
Provedor de dados: Genetics and Molecular Biology
Autores: Baptista,Juliana Cristina; Machado,Marcos Antonio; Homem,Rafael Augusto; Torres,Pablo Sebastián; Vojnov,Adrian Alberto; Amaral,Alexandre Morais do.
The Gram-negative bacterium Xanthomonas axonopodis pv. citri, the causal agent of citrus canker, is a major threat to the citrus industry worldwide. Although this is a leaf spot pathogen, it bears genes highly related to degradation of plant cell walls, which are typically found in plant pathogens that cause symptoms of tissue maceration. Little is known on Xac capacity to cause disease and hydrolyze cellulose. We investigated the contribution of various open reading frames on degradation of a cellulose compound by means of a global mutational assay to selectively screen for a defect in carboxymethyl cellulase (CMCase) secretion in X. axonopodis pv. citri. Screening on CMC agar revealed one mutant clone defective in extracellular glycanase activity, out of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus canker; Transposome; Type II secretion system.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100025

Dominance, epistasis, heritabilities and expected genetic gains
Provedor de dados: Genetics and Molecular Biology
Autores: Viana,José Marcelo Soriano.
Although epistasis is common in gene systems that determine quantitative traits, it is usually not possible to estimate the epistatic components of genotypic variance because experiments in breeding programs include only one type of progeny. As the study of this phenomenon is complex, there is a lack of theoretical knowledge on the contribution of the epistatic variances when predicting gains from selection and on the bias in estimating genetic parameters when fitting the additive-dominant model. The objective of this paper is to discuss these aspects. Regarding a non-inbred population, the genetic value due to dominance and the epistatic components of the genotypic value are not indicators of the number of favorable genes present in an individual. Thus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breeding; Genetic parameters; Gene interaction; Selection.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100012

Genetic analysis of soybean resistance to Fusarium solani f.sp. glycines
Provedor de dados: Genetics and Molecular Biology
Autores: Fronza,Vanoli; Vello,Natal Antonio; Camargo,Luis Eduardo Aranha.
In order to study the genetic control of soybean resistance to sudden death syndrome (SDS), a 5 x 5 diallel with the F2 generation, without the reciprocals, was carried out in a greenhouse. The following parents were used: Forrest, MG/BR-46 (Conquista), IAC-4, FT-Cristalina, and FT-Estrela. The first two cultivars are more resistant to SDS than IAC-4, which is considered to be moderately resistant to SDS, and the last two cultivars are highly susceptible. The fungus was inoculated with three colonized sorghum grains placed at the bottom of the holes with two soybean seeds. Single plants were evaluated between 14 and 37 days after emergency based on foliar severity symptoms (1-5) of SDS. The disease incidence and a disease index were also calculated for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Resistance; Sudden death syndrome-SDS; Diallel crosses; Jinks-Hayman; Molecular markers SSR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300016

Analysis of the sex ratio in Bradysia matogrossensis (Diptera, Sciaridae)
Provedor de dados: Genetics and Molecular Biology
Autores: Rocha,Lincoln S.; Perondini,André Luiz P.
In sciarid flies, the control of sex determination and of the progeny sex ratio is exercised by the parental females, and is based on differential X-chromosome elimination in the initial stages of embryogenesis. In some species, the females produce unisexual progenies (monogenic females) while in others, the progenies consist of males and females (digenic females). The sex ratio of bisexual progenies is variable, and departs considerably from 1:1. Bradysia matogrossensis shows both monogenic and digenic reproduction. In a recently established laboratory strain of this species, 15% of the females were digenic, 10% produced only females, 13% produced only males, and 62% produced progenies with one predominant sex (33% predominantly of female and 29%...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100018

Phylogenetic niche conservatism and plant diversification in South American subtropical grasslands along multiple climatic dimensions
Provedor de dados: Genetics and Molecular Biology
Autores: Barros,Michel J.F.; Silva-Arias,Gustavo A.; Segatto,Ana Lúcia Anversa; Reck-Kortmann,Maikel; Fregonezi,Jeferson N.; Diniz-Filho,José Alexandre F.; Freitas,Loreta B.
Abstract Phylogenetic niche conservatism can be investigated at multiple scales on an explicit geographical context. Haplotype-based comparative analyses of lineages occupying the same region, and thus subjected to similar environmental factors, allow decoupling shared evolutionary and ecological patterns, as well as multiple dimensions of adaptive diversification. Here we aimed to assess the role of environmental drivers on diversification of subtropical grassland, based on haplotypic diversity of two plant genera. We sampled two closely related and co-distributed grassland plant genera, Petunia and Calibrachoa, across their entire distribution area. Eigenvectors extracted from pairwise distances based on chloroplast DNA haplotypes were used to fit...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Altitude; Climatic changes; Molecular diversification; Phylogenetic niche conservatism.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400602

Identification of differentially expressed genes of Xanthomonas axonopodis pv. citri by representational difference analysis of cDNA
Provedor de dados: Genetics and Molecular Biology
Autores: Mehta,Angela; Rosato,Yoko B.
Xanthomonas axonopodis pv. citri is a phytopathogenic bacterium responsible for citrus canker, a serious disease which causes severe losses in citriculture around the world. In this study we report the differential expression of X. axonopodis pv. citri in response to specific treatments by using Representational Difference Analysis of cDNA (cDNA RDA). cDNAs from X. axonopodis pv. citri cultured in the presence of leaf extract of the host plant (Citrus sinensis), in vivo, as well as in the complex medium were hybridized against cDNA of the bacterium grown in the minimal medium. Sequencing of the difference products obtained after the second and third hybridizations revealed a total of 37 distinct genes identified by homology searches in the genome of X....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus canker; Differential expression; Leaf extract; In vivo.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100024

Expression of miR-148b-3p is correlated with overexpression of biomarkers in prostate cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Arámbula-Meraz,Eliakym; Bergez-Hernández,Fernando; Leal-León,Emir; Romo-Martínez,Enrique; Picos-Cárdenas,Verónica; Luque-Ortega,Fred; Romero-Quintana,Jose; Alvarez-Arrazola,Marco; García-Magallanes,Noemí.
Abstract Prostate cancer (PCa) is one of the leading causes of death among men. Genes such as PCA3, PSA, and Fra-1 are suggested to serve as potential tools for the detection of PCa, as they are deregulated during this pathology. A similar event occurs with small non-coding RNAs, called miRNAs, specifically miR-195-5p, miR-133a-3p, and miR-148b-3p, which were analyzed in a Chinese population and suggested to be possible candidates for PCa diagnosis. We evaluated the expression levels of three miRNAs and three genes in tissue samples of PCa and benign prostate disease, such as benign prostatic hyperplasia, or prostatitis, in order to determine their potential as candidates for PCa detection. Our results showed a statistically significant overexpression of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; Biomarker; MiRNAs; Correlation; Prostate cancer.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100112

The paralysé (par) mouse neurological mutation maps to a 9 Mbp (4 cM) interval of mouse chromosome 18
Provedor de dados: Genetics and Molecular Biology
Autores: Silva Neto,Lino; Aghaie,Asadollah; Guénet,Jean-Louis; Godard,Ana Lúcia Brunialti.
The Paralysé mutation is a spontaneous neuromuscular mutation, first observed in 1980 at the Pasteur Institute, which is transmitted by the autosomal recessive par allele. Affected homozygote par/par mice rarely survive beyond 16 days of age and at the end of their life they are emaciated and completely paralyzed. Several concordant histological and physiological observations indicate that mutant mice might be good models for studying early-onset human motor neuron diseases such as spinal muscular atrophy. Linkage analysis using a set of molecular markers and two F2 crosses indicate that the mutation maps to mouse chromosome 18 in a region spanning 4 cM (or 9 megabase pairs, Mbp) between the microsatellites D18Mit140 and D18Mit33. These results positioned...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mouse model; Neuromuscular disease; Mouse genetic map; Paralysé mutation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200003

Artificial infestation of Boophilus microplus in beef cattle heifers of four genetic groups
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Ana Mary da; Alencar,Maurício Mello de; Regitano,Luciana Correia de Almeida; Oliveira,Márcia Cristina de Sena; Barioni Júnior,Waldomiro.
Resistance of beef cattle heifers to the cattle tick Boophilus microplus was evaluated by artificial infestation of 66 beef cattle heifers of the following genetic groups: 16 Nelore (NE), 18 Canchim x Nelore (CN), 16 Angus x Nelore (AN) and 16 Simmental x Nelore (SN). The animals, with a mean age of 16.5 months, were maintained with no chemical tick control in a Brachiaria decumbens pasture. Four artificial infestations with 20,000 B. microplus larvae were carried out 14 days apart and from day 18 to day 22 of each infestation the number of engorged female ticks (&gt; 4.5 mm) was counted on the left side of each heifer. Data were analyzed as the percentage of return (PR = percentage of ticks counted relative to the number infested), transformed to (PR)¼,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Crossbred; Tick resistance.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600020

The lipidome, genotoxicity, hematotoxicity and antioxidant properties of andiroba oil from the Brazilian Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Milhomem-Paixão,Susana Suely Rodrigues; Fascineli,Maria Luiza; Roll,Mariana Matos; Longo,João Paulo Figueiró; Azevedo,Ricardo Bentes; Pieczarka,Julio Cesar; Salgado,Hugo Leonardo Crisóstomo; Santos,Alberdan Silva; Grisolia,Cesar Koppe.
Abstract Andirobeira is an Amazonian tree, the seeds of which produce a commercially valuable oil that is used in folk medicine and in the cosmetic industry. Andiroba oil contains components with anti-inflammatory, cicatrizing and insect-repellant actions. However, virtually nothing is known of the safety of this oil for humans. The aim of this work was therefore to investigate the hematotoxicity, genotoxicity and mutagenicity of andiroba oil using the comet and micronucleus assays, and to assess its antioxidant properties and lipidome as a means of addressing safety issues. For the experiments, andiroba oil was administered by gavage for 14 consecutive days in nulliparous female Swiss mice randomly distributed in four groups: negative control and three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute toxicity; Antioxidant activity; Carapa guianensis; Genotoxicity; Lipidomics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200248

Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus)
Provedor de dados: Genetics and Molecular Biology
Autores: Armstrong,Eileen; Postiglioni,Alicia; Martínez,Amparo; Rincón,Gonzalo; Vega-Pla,José Luis.
The Uruguayan Creole cattle genetic reserve consists of a herd of about 600 animals (bulls, cows and calves) located in an indigenous habitat of 650 hectares. In a previous study, a random sample from this herd showed high heterozygosity and a Hardy-Weinberg equilibrium for markers of major genes related to milk production. To study its genetic diversity we genotyped a sample of bulls (N = 19 out of 23 for the whole herd) using the PCR reaction with a set of 17 microsatellite markers. Between two and seven different alleles were identified per microsatellite in a total of 73 alleles. The expected mean heterozygosity (He) per locus was between 0.465 and 0.801, except for microsatellite HEL13 which gave a He value of 0.288. The expected mean heterozygosity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uruguayan Creole cattle; Genetic diversity; Microsatellites.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200012

Effects of cytoplasmic inheritance on preweaning traits of Hereford cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Mezzadra,Carlos Alberto; Melucci,Lilia Magdalena; Corva,Pablo Marcelo; López Valiente,Sebastián; Rípoli,María Verónica; Lirón,Pedro; Giovambattista,Guillermo.
The influence of cytoplasmic inheritance on birth and weaning weight was evaluated in an experimental Hereford herd. Data on 1,720 records for birth and weaning weights from calves born between 1963 and 2002 were studied. Variance components were estimated using MTDFREML procedures and an animal model was fitted for each trait. Direct and maternal additive effects and permanent environment and maternal lineage effects were treated as random, while year and month of birth, age of dam and sex of the calf were treated as fixed. Identification of maternal lineages was based on pedigree information. The contribution to phenotypic variance of cytoplasmic lineages defined by pedigree information was negligible for both traits. Mitochondrial genotypes of cows...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Hereford; Birth weight; Weaning weight; Cytoplasmic inheritance; SSCP.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300003

Alterations in DNA repair gene expression and their possible regulation in rat-liver regeneration
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,Gai-Ping; Xu,Cun-Shuan.
Rapidly proliferating tissue may require enhanced DNA repair capacity in order to avoid fixation of promutagenic DNA lesions to mutations. Partial hepatectomy (PH) triggers cell proliferation during liver regeneration (LR). However, little is known on how DNA repair genes change and how they are regulated at the transcriptional level during LR. In the present study, the Rat Genome 230 2.0 array was used to detect the expression profiles of DNA repair genes during LR, and differential expression of selected genes was confirmed by real-time RT-PCR. 69 DNA repair genes were found to be associated with LR, more than half of which distributed in a cluster characterized by a gradual increase at 24-72h and then returning to normal. The expression of base excision...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Partial hepatectomy; Rat genome array; DNA repair genes; Liver regeneration.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200023

Mucopolysaccharidosis I, II, and VI: brief review and guidelines for treatment
Provedor de dados: Genetics and Molecular Biology
Autores: Giugliani,Roberto; Federhen,Andressa; Muñoz Rojas,Maria Verônica; Vieira,Taiane; Artigalás,Osvaldo; Lapagesse Pinto,Louise; Azevedo,Ana Cecília; Acosta,Angelina; Bonfim,Carmen; Lourenço,Charles Marques; Chong Ae,Kim; Horovitz,Dafne; Bonfim,Denize; Norato,Denise; Marinho,Diane; Palhares,Durval; Santos,Emerson Santana; Ribeiro,Erlane; Valadares,Eugênia; Guarany,Fábio; Lucca,Gisele Rosone de; Pimentel,Helena; Souza,Isabel Neves de; Correa Neto,Jordão; Fraga,José Carlos; Goes,José Eduardo; Cabral,José Maria; Simionato,José; Llerena Jr.,Juan; Jardim,Laura; Giuliani,Liane; Silva,Luiz Carlos Santana da; Santos,Mara L.; Moreira,Maria Angela; Kerstenetzky,Marcelo; Ribeiro,Márcia; Ruas,Nicole; Barrios,Patricia; Aranda,Paulo; Honjo,Rachel; Boy,Raquel; Costa,Ronaldo; Souza,Carolina; Alcantara,Flavio F.; Avilla,Silvio Gilberto A.; Fagondes,Simone; Martins,Ana Maria.
Mucopolysaccharidoses (MPS) are rare genetic diseases caused by the deficiency of one of the lysosomal enzymes involved in the glycosaminoglycan (GAG) breakdown pathway. This metabolic block leads to the accumulation of GAG in various organs and tissues of the affected patients, resulting in a multisystemic clinical picture, sometimes including cognitive impairment. Until the beginning of the XXI century, treatment was mainly supportive. Bone marrow transplantation improved the natural course of the disease in some types of MPS, but the morbidity and mortality restricted its use to selected cases. The identification of the genes involved, the new molecular biology tools and the availability of animal models made it possible to develop specific enzyme...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucopolisaccharidoses; Hurler syndrome; Hunter syndrome; Maroteaux-Lamy syndrome; Enzyme replacement therapy; Treatment guidelines.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400001

Studies on diversity and evolution of Iridaceae species in southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Souza-Chies,Tatiana T.; Santos,Eliane Kaltchuk dos; Eggers,Lilian; Flores,Alice Mainieri; Alves,Eudes M. Stiehl; Fachinetto,Juliana; Lustosa,Juliana; Corrêa,Lauís Brisolara; Tacuatiá,Luana Olinda; Piccoli,Paula; Miz,Rogéria Beatriz.
Plants of the family Iridaceae are well represented in the grassland vegetation of southern Brazil, occurring in the Pampa and Atlantic Forest biomes. Nevertheless, little is known about the taxonomy and evolution of Iridaceae species in southern Brazil. The main goal of this review is to compile published information about South American Iridaceae, and to discuss the evolution and genetic diversity of the family presenting our own research data in the light of the published literature. The main focus is on the genera Calydorea, Cypella, Herbertia, and Sisyrinchium. Aspects of reproductive system and of pollinator attraction are also discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotaxonomy; Molecular phylogenetics; Iridoideae; Population genetics; Diversity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600018

Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress
Provedor de dados: Genetics and Molecular Biology
Autores: Palmieri,Ana Carolina Basílio; Amaral,Alexandre Morais do; Homem,Rafael Augusto; Machado,Marcos Antonio.
In this study, we used real-time quantitative PCR (RT-qPCR) to evaluate the expression of 32 genes of Xanthomonas axonopodis pv. citri related to pathogenicity and virulence that are also involved in copper detoxification. Nearly all of the genes were up-regulated, including copA and copB. Two genes homologous to members of the type II secretion system (xcsH and xcsC) and two involved in the degradation of plant cell wall components (pglA and pel) were the most expressed in response to an elevated copper concentration. The type II secretion system (xcs operon) and a few homologues of proteins putatively secreted by this system showed enhanced expression when the bacteria were exposed to a high concentration of copper sulfate. The enhanced expression of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copper resistance; Citrus canker; Real-time quantitative PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200024

PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2
Provedor de dados: Genetics and Molecular Biology
Autores: Guo,Qinggang; Li,Shezeng; Lu,Xiuyun; Li,Baoqing; Ma,Ping.
The Bacillus subtilis strain NCD-2 is an important biocontrol agent against cotton verticillium wilt and cotton sore shin in the field, which are caused by Verticillium dahliae Kleb and Rhizoctonia solani Kuhn, respectively. A mutant of strain NCD-2, designated M216, with decreased antagonism to V. dahliae and R. solani, was selected by mini-Tn10 mutagenesis and in vitro virulence screening. The inserted gene in the mutant was cloned and identified as the phoR gene, which encodes a sensor kinase in the PhoP/PhoR two-component system. Compared to the wild-type strain, the APase activities of the mutant was decreased significantly when cultured in low phosphate medium, but no obvious difference was observed when cultured in high phosphate medium. The mutant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus subtilis; Antagonism; Verticillium dahliae; Mini-Tn10.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200022

Differential expression of the pr1A gene in Metarhizium anisopliae and Metarhizium acridum across different culture conditions and during pathogenesis
Provedor de dados: Genetics and Molecular Biology
Autores: Leão,Mariele Porto Carneiro; Tiago,Patricia Vieira; Andreote,Fernando Dini; de Araújo,Welington Luiz; de Oliveira,Neiva Tinti.
The entomopathogenic fungi of the genus Metarhizium have several subtilisin-like proteases that are involved in pathogenesis and these have been used to investigate genes that are differentially expressed in response to different growth conditions. The identification and characterization of these proteases can provide insight into how the fungus is capable of infecting a wide variety of insects and adapt to different substrates. In addition, the pr1A gene has been used for the genetic improvement of strains used in pest control. In this study we used quantitative RT-PCR to assess the relative expression levels of the pr1A gene in M. anisopliae and M. acridum during growth in different culture conditions and during infection of the sugar cane borer,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogen; Diatraea saccharalis; Quantitative RT-PCR; Expression pattern.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100086

Restriction fragment length polymorphisms of mitochondrial DNA among five freshwater fish species of the genus Astyanax (Pisces, Characidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Moysés,Cinthia Bachir; Almeida-Toledo,Lurdes F. de.
Restriction fragment length polymorphism (RFLP) analysis of mitochondrial DNA (mtDNA) was employed to characterize species and populations of Astyanax, a Neotropical freshwater fish genus. Samples of five species, A. altiparanae, A. fasciatus, A. lacustris, A. scabripinnis paranae and A. schubarti, from the Upper Paraná and São Francisco river basins were analyzed. Two out of the ten restriction enzymes employed generated species-specific mtDNA patterns for each of the five species. MtDNA exhibited considerable polymorphism within and among populations. All populations sampled showed relatively high values of haplotype diversity. Geographically localized haplotypes were detected for A. altiparanae and A. fasciatus from the Upper Paraná and São Francisco...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; RFLP; Astyanax.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400008

Partitioning genetic effects due to embryo, cytoplasm and maternal parent for oil content in oilseed rape (Brassica napus L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Wu,Jian-Guo; Shi,Chun-Hai; Zhang,Hai-Zhen.
Analysis of genetic main effects and genotype x environment (GE) interaction effects on the oil content of oilseed rape (Brassica napus L.) was conducted by using a genetic model for the quantitative traits of seeds in diploid plants. The experiments were carried out over two years with 8 parents and a diallel mating design, which produced F1 and F2 generations. We found that the oil content of rape was simultaneously controlled by embryo genetic effect, cytoplasmic effects and maternal genetic effect as well as GE interaction effects, with the cytoplasmic and maternal effects playing the main role. The results indicate that selection of maternal plants for high oil content would be more efficient than selection based on single seeds. Since the GE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breeding selection; Environmental interactions; Heritability; QTL.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300023

Protection by Panax ginseng C.A. Meyer against the genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Denise G.; Antunes,Lusânia M.G.; Graf,Ulrich; Spanó,Mário A.
Panax ginseng is one of the most widely prescribed herbal medicines for the treatment of cancer, diabetes, chronic inflammation, and neurodegenerative and cardiovascular diseases. Since the use of alternative medicines in combination with conventional therapy may increase the risk of unwanted interactions, we investigated the possible genotoxicity of a water-soluble form of the dry root of P. ginseng (2.5, 5.0 or 10.0 mg/mL) and its ability to protect against the genotoxicity of doxorubicin (DOX; 0.125 mg/mL) by using the Drosophila melanogaster wing somatic mutation and recombination test (SMART) with standard and high-bioactivation crosses of flies. Panax ginseng was not genotoxic at the concentrations tested, whereas DOX-induced genotoxicity in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigenotoxicity; SMART; Wing spot test.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500024

A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis
Provedor de dados: Genetics and Molecular Biology
Autores: Zhang,Lu; Hu,Weijuan; Gao,Yike; Pan,Huitang; Zhang,Qixiang.
Abstract We cloned and characterized the full-length coding sequence of a small heat shock (sHSP) gene, PfHSP17.2, from Primula forrestii leaves following heat stress treatment. Homology and phylogenetic analysis suggested that PfHSP17.2 is a cytosolic class II sHSP, which was further supported by the cytosolic localization of transient expression of PfHSP17.2 fused with green fluorescent protein reporter. Expression analysis showed that PfHSP17.2 was highly inducible by heat stress in almost all the vegetative and generative tissues and was expressed under salt, cold, and oxidative stress conditions as well. Moreover, the expression of PfHSP17.2 in P. forrestii was detected in certain developmental growth stages. Transgenic Arabidopsis thaliana...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SHSPs; Stress tolerance; Arabidopsis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400649

Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects
Provedor de dados: Genetics and Molecular Biology
Autores: Mendonça,Leonardo Meneghin; Machado,Carla da Silva; Teixeira,Cristiane Cardoso Correia; Freitas,Luis Alexandre Pedro de; Bianchi,Maria Lourdes Pires; Antunes,Lusânia Maria Greggi.
Abstract Curcumin (CMN) is the principal active component derived from the rhizome of Curcuma longa (Curcuma longa L.). It is a liposoluble polyphenolic compound that possesses great therapeutic potential. Its clinical application is, however, limited by the low concentrations detected following oral administration. One key strategy for improving the solubility and bioavailability of poorly water-soluble drugs is solid dispersion, though it is not known whether this technique might influence the pharmacological effects of CMN. Thus, in this study, we aimed to evaluate the antioxidant and antigenotoxic effects of CMN formulated in a solid dispersion (CMN SD) compared to unmodified CMN delivered to Wistar rats. Cisplatin (cDDP) was used as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcuma longa; Antigenotoxicity; Micronucleus test; DNA damage; Comet assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400490

MicroRNA-21 over expression in umbilical cord blood hematopoietic stem progenitor cells by leukemia microvesicles
Provedor de dados: Genetics and Molecular Biology
Autores: Razmkhah,Farnaz; Soleimani,Masoud; Ghasemi,Sorayya; Kafi-abad,Sedigheh Amini.
Abstract Microvesicles are able to induce the cell of origin’s phenotype in a target cell. MicroRNA-21, as an oncomir, is up-regulated in almost all cancer types such as leukemia which results in cell proliferation. In this study, we examine the ability of leukemia microvesicles to induce proliferation in hematopoietic stem progenitor cells (HSPCs) via microRNA-21 dysregulation. Herein, leukemia microvesicles were isolated from HL-60 and NB-4 cell lines by ultracentrifugation, and then their protein content was measured. Normal HSPCs were isolated from umbilical cord blood samples by a CD-34 antibody. These cells were treated with 20 and 40 μg/mL leukemia microvesicles for 5 and 10 days, respectively. Cell count, CD-34 analysis, and a microRNA-21 gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukemia; Microvesicles; Hematopoietic stem cells; MicroRNA-21.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300465

Transposable elements from the mesophragmatica group of Drosophila
Provedor de dados: Genetics and Molecular Biology
Autores: Germanos,Erika; Mota,Nina R.; Loreto,Elgion L.S.
Transposable elements (TEs) are middle repetitive DNA sequences classified into families according to their sequence similarities, such elements can playing an important role in the evolutionary process of their host genomes. There are many reports on the distribution of TEs in the fruit fly genus Drosophila, although there is relatively little information relating to the Neotropical mesophragmatica group of Drosophila, probably the most typical cluster of species occurring almost exclusively in the Andes mountains. Dot Blot and PCR analyses was used to study the distribution of some TEs (I, mariner, hobo, gypsy, Tom/17.6, micropia and P elements) within the mesophragmatica group of Drosophila. We found gypsy elements in all the mesophragmatica group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila; Mesophragmatica; Phylogenetic distribution; Transposable elements.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400026

The genetics of tolerance to tristeza disease in citrus rootstocks
Provedor de dados: Genetics and Molecular Biology
Autores: Bordignon,Rita; Medina-Filho,Herculano Penna; Siqueira,Walter José; Teófilo Sobrinho,Joaquim.
Controlled pollinations between four elite citrus rootstocks, Citrus limonia - 'Limeira' rangpur lime (Cravo), C. sunki - 'Sunki' mandarin (Sunki), C. aurantium - 'São Paulo' sour orange (Azeda) and Poncirus trifoliata - 'Davis A' trifoliate orange (Trifoliata), resulted in 1614 nucelar and 1938 hybrid plants identified by the isozyme loci Pgi-1, Pgm-1, Got-1, Got-2, Aps-1, Me-1, Prxa-1 and or by the morphological markers broadness of leaf petiole wing or trifoliolate leaves. Tolerance to the citrus tristeza virus (CTV) was evaluated under nursery and field conditions for several years by the reaction of Valencia orange infected with a severe strain of CTV and grafted onto the hybrids and nucellar clones. Genetic analyses indicated that tolerance was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; CTV; Epistasis; Rootstock; Tristeza.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200013

Partial diallel analysis of agronomic characters in rice (Oryza sativa L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Torres,Edgar Alonso; Geraldi,Isaias O.
Rice (Oryza sativa L.) breeding seeking to combine high productivity and cold tolerance for the temperate Latin America region is an important challenge. We estimated some useful parameters which can be used to investigate the genetic control of agronomic characters in crosses combining cold tolerance and productivity. A partial diallel design was used in crosses between six tropical indica rice cold susceptible genotypes (group 1) and seven japonica or indica/japonica cold tolerant rice genotypes (group 2). Parents and crosses were evaluated for agronomic characters under field conditions in two different experiments in 2005. The results showed significant mid-parent heterosis for all characters (plant height, tiller number, days to 50% flowering, panicle...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: General combining ability; Heterosis; Hybrid rice; Specific combining ability.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400018

Guidelines for diagnosis and treatment of Hunter Syndrome for clinicians in Latin America
Provedor de dados: Genetics and Molecular Biology
Autores: Giugliani,Roberto; Villarreal,Martha Luz Solano; Valdez,C. Araceli Arellano; Hawilou,Antonieta Mahfoud; Guelbert,Norberto; Garzón,Luz Norela Correa; Martins,Ana Maria; Acosta,Angelina; Cabello,Juan Francisco; Lemes,Aída; Santos,Mara Lucia Schmitz Ferreira; Amartino,Hernán.
This review aims to provide clinicians in Latin America with the most current information on the clinical aspects, diagnosis, and management of Hunter syndrome, a serious and progressive disease for which specific treatment is available. Hunter syndrome is a genetic disorder where iduronate-2-sulfatase (I2S), an enzyme that degrades glycosaminoglycans, is absent or deficient. Clinical manifestations vary widely in severity and involve multiple organs and tissues. An attenuated and a severe phenotype are recognized depending on the degree of cognitive impairment. Early diagnosis is vital for disease management. Clinical signs common to children with Hunter syndrome include inguinal hernia, frequent ear and respiratory infections, facial dysmorphisms,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hunter syndrome; Lysosomal disease; Iduronate-2-sulfatase; Enzyme replacement therapy; Treatment guidelines.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300003

Analysis of tRNA halves (tsRNAs) in serum from cattle challenged with bovine viral diarrhea virus
Provedor de dados: Genetics and Molecular Biology
Autores: Taxis,Tasia M.; Bauermann,Fernando V.; Ridpath,Julia F.; Casas,Eduardo.
Abstract Acute infections of bovine viral diarrhea virus (BVDV) lead to a range of clinical presentations. Laboratory tests for detection depend on collection of samples during a short viremia. Acutely infected animals remain largely undiagnosed. Transfer RNA halves (tsRNAs) are hypothesized to function like microRNAs to regulate gene expression during an immune response. The objective of this study was to identify tsRNAs in cattle that had been challenged with a non-cytopathic field strain of BVDV. Colostrum-deprived neonatal Holstein calves were either challenged with BVDV (n=5) or mock challenged (n=4). Sera was collected prior to challenge and days 4, 9, and 16 post challenge. RNA was extracted and read counts of small non-coding RNAs were assessed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Bovine Viral Diarrhea Virus; Transfer RNA; Transfer RNA halves; Small non-coding RNA.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300374

A new concept of relatedness and its significance to the theory of social evolution
Provedor de dados: Genetics and Molecular Biology
Autores: Drummond,M.S.
Data on primitively social groups of Hymenoptera have been somewhat contradictory with respect to kin recognition, degree of relatedness and social evolution. This study presents a new concept of "relatedness coefficient". Called "aggregated relatedness", the hypothesis here introduced proposes that genes shared by any two individuals affect formation of family units to an extent dependent on their frequency and manner of dispersion in neighboring populations.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100008

Expression of heterosis for productive traits in F1 eggplant (Solanum melongena L.) hybrids
Provedor de dados: Genetics and Molecular Biology
Autores: Sousa,João Alencar de; Maluf,Wilson Roberto.
This study was carried out to obtain estimates of heterosis in crosses between seven eggplant cultivars (Embu = E; Santa Genebra = SG; Viserba = V; Aubergine de Barbentane = AB; Florida Market 10 = FM; Black Beauty = BB, and Melitino = M) and two breeding lines (B-14-07 = B1 and B-31-06 = B2). The F1 hybrids used were: E x FM; E x BB; E x M; E x B1; E x B2; SG x FM; SG x BB; SG x M; SG x B1; SG x B2; V x FM; V x B1; V x B2; AB x FM; AB x M; AB x B1; AB x B2 and M x FM. Cultivars, lines and hybrids were evaluated at the ESAL experimental field in Lavras, MG, from February to October 1992. A randomized complete block design with three replications was used. Significant heterosis relative to the parental means was detected for all traits studied. Their values...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100017

An AFLP marker linked to the Pm-1 gene that confers resistance to Podosphaera xanthii race 1 in Cucumis melo
Provedor de dados: Genetics and Molecular Biology
Autores: Teixeira,Ana Paula Matoso; Barreto,Fatima Aparecida da Silva; Camargo,Luis Eduardo Aranha.
Brazil produced 330,000 metric tons of melons in 2005, principally in the Northeast region where one of the most important melon pathogens is the powdery mildew fungus Podosphaera xanthii. The disease is controlled mainly by incorporating single dominant resistance genes into commercial hybrids. We report on linkage analysis of the Pm-1 resistance gene, introgressed from the AF125Pm-1 Cantalupensis Charentais-type breeding line into the yellow-fleshed melon (Group Inodorus) breeding line AF426-S by backcrossing to produce the resistant line AF426-R, and the amplified fragment length polymorphism (AFLP) marker M75/H35_155 reported to be polymorphic between AF426-S and AF426-R. Segregation analysis of M75/H35_155 using a backcross population of 143 plants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP marker; Cucumis melo; Disease resistance; Linkage analysis; Powdery mildew.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300023

DNA repair genes in astrocytoma tumorigenesis, progression and therapy resistance
Provedor de dados: Genetics and Molecular Biology
Autores: Sousa,Juliana Ferreira de; Serafim,Rodolfo Bortolozo; Freitas,Laura Marise de; Fontana,Carla Raquel; Valente,Valeria.
Abstract Glioblastoma (GBM) is the most common and malignant type of primary brain tumor, showing rapid development and resistance to therapies. On average, patients survive 14.6 months after diagnosis and less than 5% survive five years or more. Several pieces of evidence have suggested that the DNA damage signaling and repair activities are directly correlated with GBM phenotype and exhibit opposite functions in cancer establishment and progression. The functions of these pathways appear to present a dual role in tumorigenesis and cancer progression. Activation and/or overexpression of ATRX, ATM and RAD51 genes were extensively characterized as barriers for GBM initiation, but paradoxically the exacerbated activity of these genes was further associated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glioblastoma; DNA repair; Biomarkers; Tumor progression; Therapy resistance.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200306

Differential allelic expression of IL13 and CSF2 genes associated with asthma
Provedor de dados: Genetics and Molecular Biology
Autores: Burkhardt,Jana; Kirsten,Holger; Wolfram,Grit; Quente,Elfi; Ahnert,Peter.
An important area of genetic research is the identification of functional mechanisms in polymorphisms associated with diseases. A highly relevant functional mechanism is the influence of polymorphisms on gene expression levels (differential allelic expression, DAE). The coding single nucleotide polymorphisms (SNPs) CSF2rs25882 and IL13rs20541 have been associated with asthma. In this work, we investigated whether the mRNA expression levels of CSF2 or IL13 were correlated with these SNPs. Samples were analyzed by mass spectrometry-based quantification of gene expression. Both SNPs influenced gene expression levels (CSF2rs25882: p overall = 0.008 and pDAE samples = 0.00006; IL13rs20541: p overall = 0.059 and pDAE samples = 0.036). For CSF2, the expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candidate gene; Gene expression; Gene polymorphism; QTL.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400004

Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis
Provedor de dados: Genetics and Molecular Biology
Autores: Almeida-Val,Vera Maria Fonseca; Oliveira,Alice Reis; Silva,Maria de Nazaré Paula da; Ferreira-Nozawa,Monica Stropa; Araújo,Roziete Mendes; Val,Adalberto Luis; Nozawa,Sérgio Ricardo.
Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such as erythropoietin, transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the LDH-A gene after an Amazonian fish species, Astronotus crassipinis (the Oscar), was exposed to hypoxia and anoxia. In earlier studies, we have discovered that adults of this species are extremely tolerant to hypoxia and anoxia, while the juveniles are less tolerant. Exposure of juveniles to acute hypoxia and anoxia resulted in increased LDH-A gene expression in skeletal and cardiac muscles. When exposed to graded hypoxia juveniles show decreased LDH-A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon; Fishes; Gene expression; RT-PCR; LDH-A; Hypoxia; Anoxia.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200025

ADRB2 polymorphisms predict the risk of myocardial infarction and coronary artery disease
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,Dong-Wei; Liu,Min; Wang,Ping; Zhan,Xiang; Liu,Yu-Qing; Zhao,Luo-Sha.
Abstract Recently, the rs1042713 G &gt; A and rs1042714 C &gt; G polymorphisms in the beta-2 adrenergic receptor (ADRB2) gene were shown to be related to atherosclerosis diseases. Therefore, we performed a systemic meta-analysis to determine whether the two functional polymorphisms are related to the risk of myocardial infarction (MI) and coronary artery disease (CAD). We identified published studies that are relevant to our topic of interest. Seven case-control studies, with a total of 6,843 subjects, were incorporated into the current meta-analysis. Our analysis showed a higher frequency of rs1042713 G &gt; A variant in patients with MI or CAD compared to healthy controls. A similar result was also obtained with the rs1042714 C &gt; G variant under both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta-2 adrenergic receptor; Genetic polymorphism; Myocardial infarction; Coronary artery disease; Meta-analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400433

High-throughput nucleotide sequence analysis of diverse bacterial communities in leachates of decomposing pig carcasses
Provedor de dados: Genetics and Molecular Biology
Autores: Yang,Seung Hak; Lim,Joung Soo; Khan,Modabber Ahmed; Kim,Bong Soo; Choi,Dong Yoon; Lee,Eun Young; Ahn,Hee Kwon.
The leachate generated by the decomposition of animal carcass has been implicated as an environmental contaminant surrounding the burial site. High-throughput nucleotide sequencing was conducted to investigate the bacterial communities in leachates from the decomposition of pig carcasses. We acquired 51,230 reads from six different samples (1, 2, 3, 4, 6 and 14 week-old carcasses) and found that sequences representing the phylum Firmicutes predominated. The diversity of bacterial 16S rRNA gene sequences in the leachate was the highest at 6 weeks, in contrast to those at 2 and 14 weeks. The relative abundance of Firmicutes was reduced, while the proportion of Bacteroidetes and Proteobacteria increased from 3–6 weeks. The representation of phyla was restored...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pig; Decomposition; Leachate; Bacterial community; Pyrosequencing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300373

c.G2114A MYH9 mutation (DFNA17) causes non-syndromic autosomal dominant hearing loss in a Brazilian family
Provedor de dados: Genetics and Molecular Biology
Autores: Dantas,Vitor G.L.; Lezirovitz,Karina; Yamamoto,Guilherme L.; Souza,Carolina Fischinger Moura de; Ferreira,Simone Gomes; Mingroni-Netto,Regina C.
We studied a family presenting 10 individuals affected by autosomal dominant deafness in all frequencies and three individuals affected by high frequency hearing loss. Genomic scanning using the 50k Affymetrix microarray technology yielded a Lod Score of 2.1 in chromosome 14 and a Lod Score of 1.9 in chromosome 22. Mapping refinement using microsatellites placed the chromosome 14 candidate region between markers D14S288 and D14S276 (8.85 cM) and the chromosome 22 near marker D22S283. Exome sequencing identified two candidate variants to explain hearing loss in chromosome 14 [PTGDR - c.G894A:p.R298R and PTGER2 - c.T247G:p.C83G], and one in chromosome 22 [MYH9, c.G2114A:p.R705H]. Pedigree segregation analysis allowed exclusion of the PTGDR and PTGER2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DFNA17; MYH9 gene; Hearing loss.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500002

Genetic analisys of a cross of gaillon (Brassica oleracea var. alboglabra) with cauliflower (B.oleracea var. botrytis)
Provedor de dados: Genetics and Molecular Biology
Autores: Spini,Vanessa B.M.G.; Kerr,Warwick Estevam.
The cauliflower (Brassica oleracea var. botrytis) is an annual vegetable cultivated in Southern and Southwestern Brazil with limited production in the Northeast and Centralwest. A variety of Chinese kale, "kaai laan" or "gaillon" (Brassica oleracea var. alboglabra), produces seeds at high temperatures and therefore can do so in North and Northeastern Brazil. Gaillon and cauliflower were crossed 55 times using 10 gaillon plants as mothers and 4 cauliflower plants as pollen donors. From these crosses, in the F2 generation, 612 plants with inflorescence like gaillon and 48 plants with inflorescence like cauliflower were obtained, in a proportion similar to 15:1, implying that 2 pairs of genes entered into formation of the cauliflower inflorescence type. In...
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100038

Antigenotoxic activity and antioxidant properties of organic and aqueous extracts of pequi fruit (Caryocar brasiliense Camb.) pulp
Provedor de dados: Genetics and Molecular Biology
Autores: Miranda-Vilela,Ana L.; Resck,Inês S.; Grisolia,Cesar K.
The daily consumption of natural antioxidants protects against oxidative damage caused by reactive oxygen species (ROS), including DNA damage, and can reduce the risk of cancer, atherosclerosis and other degenerative diseases. The pulp of pequi (Caryocar brasiliense Camb.) fruit, a tree native to the Brazilian savannah, contains several compounds with antioxidant properties, including carotenoids, vitamin C, phenolic compounds such as flavonoids, saponins and tannins, and essential oils. In this work, we examined the ability of organic and aqueous extracts of pequi fruit pulp to protect against the genotoxicity induced by two antineoplastic drugs, cyclophosphamide (CP) and bleomycin (BLM). Micronucleus tests with mouse bone marrow cells and single-cell gel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigenotoxic; Antioxidant; Caryocar brasiliense; Comet assay; Micronucleus; Pequi pulp extracts; Reactive oxygen species; TBARS assay.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500025

Association of PPARγ2 polymorphisms with carcass and meat quality traits in a Pietrain x Jinhua F2 population
Provedor de dados: Genetics and Molecular Biology
Autores: Chen,Zhe; Zhao,Xiaofeng; Jiang,Xiaoling; Guo,Xiaoling; Lv,Zunzhou; Zhou,Hongmei; Xu,Ningying.
The PPARγ2 gene is a key regulator of both proliferation and preadipocyte differentiation in mammals. Herein its genotype and allele frequencies were analyzed using PCR-SSCP in eight pig breeds (N = 416). Two kinds of polymorphisms of the PPARγ2 gene were detected, including a previously reported shift SNP A177G (Met59Val) in exon 1 and a novel silent mutation G876A in exon 5. The results revealed that European pig breeds carry a higher allele A frequency at the A177G locus and a fixed GG genotype at the G876A locus. Allele A at the G876A locus was only found in Jinhua pigs. The association between haplotype (A177G/G876A) and carcass and meat quality traits was analyzed in a Pietrain x Jinhua F2 population (N = 248). The PPARγ2 gene was found to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PPARγ2 gene; Haplotype; Carcass and meat quality; Pigs.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100011

Genotoxicity biomonitoring of sewage in two municipal wastewater treatment plants using the Tradescantia pallida var. purpurea bioassay
Provedor de dados: Genetics and Molecular Biology
Autores: Thewes,Márcia Regina; Endres Junior,Delio; Droste,Annette.
The genotoxicity of untreated and treated sewage from two municipal wastewater treatment plants (WTP BN and WTP SJN) in the municipality of Porto Alegre, in the southern Brazilian state of Rio Grande do Sul, was evaluated over a one-year period using the Tradescantia pallida var. purpurea (Trad-MCN) bioassay. Inflorescences of T. pallida var. purpurea were exposed to sewage samples in February (summer), April (autumn), July (winter) and October (spring) 2009, and the micronuclei (MCN) frequencies were estimated in each period. The high genotoxicity of untreated sewage from WTP BN in February and April was not observed in treated sewage, indicating the efficiency of treatment at this WTP. However, untreated and treated sewage samples from WTP SJN had high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Effluent; Micronucleus; Mutagenicity; Pollution; Trad-MCN.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400023

The history of the introduction of the giant river prawn, Macrobrachium cf. rosenbergii (Decapoda, Palaemonidae), in Brazil: new insights from molecular data
Provedor de dados: Genetics and Molecular Biology
Autores: Iketani,Gabriel; Pimentel,Luciana; Silva-Oliveira,Glaúcia; Maciel,Cristiana; Valenti,Wagner; Schneider,Horacio; Sampaio,Iracilda.
The giant river prawn, Macrobrachium cf. rosenbergii, is one of the most cultivated freshwater prawns in the world and has been introduced into more than 40 countries. In some countries, this prawn is considered an invasive species that requires close monitoring. Recent changes in the taxonomy of this species (separation of M. rosenbergii and M. dacqueti) require a re-evaluation of introduced taxa. In this work, molecular analyses were used to determine which of these two species was introduced into Brazil and to establish the geographic origin of the introduced populations that have invaded Amazonian coastal waters. The species introduced into Brazil was M. dacqueti through two introduction events involving prawns originating from Vietnam and either...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinvasion; Exotic species; Giant river prawn; Macrobrachium dacqueti; COI; 16S rRNA; Amazonia.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100025

Synteny of human chromosomes 14 and 15 in the platyrrhines (Primates, Platyrrhini)
Provedor de dados: Genetics and Molecular Biology
Autores: Gifalli-Iughetti,Cristiani; Koiffmann,Célia P.
In order to study the intra- and interspecific variability of the 14/15 association in Platyrrhini, we analyzed 15 species from 13 genera, including species that had not been described yet. The DNA libraries of human chromosomes 14 and 15 were hybridized to metaphases of Alouatta guariba clamitans, A. caraya, A. sara, Ateles paniscus chamek, Lagothrix lagothricha, Brachyteles arachnoides, Saguinus midas midas, Leontopithecus chrysomelas, Callimico goeldii, Callithrix sp., Cebus apella, Aotus nigriceps, Cacajao melanocephalus, Chiropotes satanas and Callicebus caligatus. The 14/15 hybridization pattern was present in 13 species, but not in Alouatta sara that showed a 14/15/14 pattern and Aotus nigriceps that showed a 15/14/15/14 pattern. In the majority of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HSA 14/15 synteny; Homologies; Chromosomal evolution; Platyrrhini; New World monkeys.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400020

Inbreeding levels in Northeast Brazil: strategies for the prospecting of new genetic disorders
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Silvana; Kok,Fernando; Weller,Mathias; Paiva,Francisco Rennan Lopes de; Otto,Paulo A.
A new autosomal recessive genetic condition, the SPOAN syndrome (an acronym for spastic paraplegia, optic atrophy and neuropathy syndrome), was recently discovered in an isolated region of the State of Rio Grande do Norte in Northeast Brazil, in a population that was identified by the IBGE (Brazilian Institute of Geography and Statistics) as belonging to the Brazilian communities with the highest rates of "deficiencies" (Neri, 2003), a term used to describe diseases, malformations, and handicaps in general. This prompted us to conduct a study of consanguinity levels in five of its municipal districts by directly interviewing their inhabitants. Information on 7,639 couples (corresponding to about 40% of the whole population of the studied districts) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inbreeding levels; Genetic drift; Geographic isolation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200003

Relationship between the Porcine Stress Syndrome gene and carcass and performance traits in F2 pigs resulting from divergent crosses
Provedor de dados: Genetics and Molecular Biology
Autores: Band,Guilherme de Oliveira; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Peixoto,Jane de Oliveira; Faria,Danielle Assis; Pires,Aldrin Vieira; Figueiredo,Frederico de Castro; Nascimento,Carlos Souza do; Gomide,Lúcio Alberto de Miranda.
The PSS genotypes of 596 F2 pigs produced by initial mating of Brazilian native boars commercial sows and were characterized by PCR-RFLP and their carcass and performance traits were evaluated. Among the 596 animals analyzed, 493 (82.72%) were characterized as NN and 103 (17.28%) as Nn. With respect to carcass traits, Nn animals presented higher (p &lt; 0.05) right half carcass weight, left half carcass weight, loin depth and loin eye area, and lower shoulder backfat thickness, backfat thickness between last and next to last but one lumbar vertebrae and backfat thickness after last rib at 6.5 cm from the midline compared to NN animals. Nn animals also showed (p &lt; 0.05) higher values for most of the cut yields, indicating higher cutting yields for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carcass; Performance; PSS gene; PCR-RFLP; Pig.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100016

Heterochromatin variation in chromosomes of Anopheles (Nyssorhynchus) darlingi Root and A.(N.) nuneztovari Gabaldón (Diptera: Culicidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Rafael,Míriam Silva; Tadei,Wanderli Pedro.
C-banding was used to study the variations in heterochromatic block markings in chromosomes of Anopheles darlingi and A. nuneztovari from Manaus, State of Amazonas, and Macapá, State of Amapá, Brazil. Both species had two differently shaped X chromosomes and a Y chromosome that was entirely heterochromatic. The X1 chromosome of A. darlingi had markings that extended 1/3 of the total length whereas in the X2 chromosome the markings were located around the centromeric region. The markings on autosomal chromosomes were concentrated in the centromeric region in both species, with a heterochromatic block in one arm of chromosome II of A. darlingi. A. nuneztovari had three heterochromatic blocks in chromosome X1 (longer) and two blocks in X2 (shorter). X2X2...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100012

Genetic variation in the cuticle-degrading protease activity of the entomopathogen Metarhizium flavoviride
Provedor de dados: Genetics and Molecular Biology
Autores: Pinto,Fabiana Gisele da Silva; Fungaro,Maria Helena Pelegrinelli; Ferreira,Josué Maldonado; Valadares-Inglis,Maria Cléria; Furlaneto,Marcia Cristina.
Extracellular proteases have been shown to be factors of virulence in fungal entomopathogenicity. We examined the production of the cuticle-degrading extracellular proteases chymoelastase (Pr1) and trypsin (Pr2) in isolates of the fungus Metarhizium flavoviride. Fungal growth was in a mineral medium (MM) containing nitrate, and in MM supplemented with either cuticle from Rhammatocerus schistocercoides or with the non-cuticular substrate casein. The substrates used for growth influenced the expression of both analyzed proteases, the highest protease activities of nearly all isolates having been observed in the medium containing insect cuticle, with more Pr1 than Pr2 being produced. There was a natural variability in the production of cuticle-degrading...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metarhizium flavoviride; Rhammatocerus schistocercoides; Chymoelastase (subtilisin) (Pr1); Trypsin (Pr2).
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200018

A new approach for obtaining rapid uniformity in rice (Oryza sativa L.) via a 3x x 2x cross
Provedor de dados: Genetics and Molecular Biology
Autores: Xing,Shaochen; Cai,Yuhong; Zhou,Kaida.
A triploid (2n = 3x = 36) rice plant was obtained by screening a twin seedling population in which each seed germinated to two or three sprouts that were then crossed with diploid plants. One diploid plant was chosen among the various F1 progenies and developed into an F2 population via self-pollination. Compared with the control variety Shanyou 63, this F2 population had a stable agronomical performance in field trials, as confirmed by the F-test. The stability of the F2 population was further substantiated by molecular analysis with simple sequence repeat markers. Specifically, of 160 markers assayed, 37 (covering all 12 chromosomes) were polymorphic between the parental lines. Testing the F1 hybrid individually with these markers showed that each PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: F-test; Polyploidy; Rice; SSR marker; Stability.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200020

Frequency of adult type-associated lactase persistence LCT-13910C/T genotypes in the Czech/Slav and Czech Roma/Gypsy populations
Provedor de dados: Genetics and Molecular Biology
Autores: Hubácek,Jaroslav A.; Adámková,Věra; Šedová,Lenka; Olišarová,Věra; Adámek,Václav; Tóthová,Valérie.
Abstract Lactase non-persistence (leading to primary lactose intolerance) is a genetically dependent inability to digest lactose in adulthood. As part of the human adaptation to dairying, the human lactase LCT-13910C/T mutation (which propagates adult expression of lactase) developed, spread and participated in the adaptation to dairying. This variant is associated with lactase activity persistence, and its carriers are able to digest lactose. We compared the frequencies of lactase 13910C/T (rs4988235) genotypes in Czechs/Slavs (N = 288) and Czech Gypsies/Roma (N = 300), two ethnically different groups where this polymorphism has not yet been analysed. Allelic frequencies significantly differed between the populations (p &lt; 0.0001). In Czechs/Slavs, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactose; Lactase persistence; Polymorphism; Czech/Slav; Czech Gypsy/Roma.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300450

Diallel crosses among maize lines with emphasis on resistance to foliar diseases
Provedor de dados: Genetics and Molecular Biology
Autores: Paterniani,Maria Elisa Ayres Guidetti Zagatto; Sawazaki,Eduardo; Dudienas,Christina; Duarte,Aildson Pereira; Gallo,Paulo Boller.
Ten elite maize (Zea mays L.) lines were crossed in a complete diallel scheme and the single-cross hybrids obtained were assessed at four experimental stations of the Agronomic Institute of Campinas, in São Paulo State, Brazil. The experiments were set up in a randomized complete block design with three replications, including four commercial checks. The experimental plots consisted of two 5-m rows spaced at 0.9 m, with a total of 50 plants. The traits assessed included: days to mid-tassel pollen shed (DPS), plant height (PH), ear height (EH), grain yield, corrected for a 50-plant stand and 14% moisture (GY corr.), and resistance to Phaeosphaeria maydis and Puccinia polysora. General and specific combining ability effects (GCA and SCA) were determined....
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200024

Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species
Provedor de dados: Genetics and Molecular Biology
Autores: Wasko,Adriane P.; Severino,Fábio E.; Presti,Flávia T.; Poletto,Andréia B.; Martins,Cesar.
An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actin gene; Amino acid residues; Gene structure; Nile tilapia; Nucleotide sequence; Oreochromis niloticus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600010

Annexin II mRNA expression in bovine oocytes during follicular development
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Luis Fabiano Santos da; Machado,Márcia Silveira Netto; Oliveira,João Francisco Coelho de; Zamberlan,Gustavo; Gonçalves,Paulo Bayard Dias.
We investigated the expression of calcium-dependent phospholipid binding protein annexin-II (Ann-II) messenger RNA (mRNA) during preantral follicle development and in oocytes from antral follicles of different diameters (&lt; 3 mm, 5 to 8 mm and &gt; 8 mm). The action of retinol on Ann-II mRNA expression in mature oocytes was also examined. Only oocytes from secondary preantral follicles expressed Ann-II mRNA and at the germinal vesicle stage expression by oocytes from follicles larger than 8 mm was significantly higher (p &lt; 0.05) compared with oocytes from follicles smaller than 3 mm or between 5 and 8 mm. Ann-II mRNA expression by metaphase II oocytes from follicles larger than 8 mm was significantly higher (p &lt; 0.05) than that from oocytes from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine oocytes; Annexin II; Retinol.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200031

Humoral immune response in adult Brazilian patients with Mucolipidosis III gamma
Provedor de dados: Genetics and Molecular Biology
Autores: Sperb-Ludwig,Fernanda; Alegra,Taciane; Velho,Renata Voltolini; Ludwig,Nataniel; Siebert,Marina; Jobim,Mariana; Vairo,Filippo; Schwartz,Ida V. D.
Abstract Mucolipidosis II and III (ML II and III) alpha/beta and ML III gamma are lysosomal diseases caused by GlcNAc-1-phosphotransferase deficiency. Previous data indicate that MLII patients have functionally impaired immune system that contributes to predisposition to infections.We evaluated the immunological phenotype of three Brazilian patients with ML III gamma. Our data suggest that the residual activity of GlcNAc-1-phosphotransferase in patients with ML III gamma is enough to allow the targeting of the lysosomal enzymes required for B-cell functions maintenance.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mucolipidosis III gamma; Inborn error of metabolism; Humoral immune response; B-cell functions.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400571

Molecular cloning, characterization and expression analysis of a novel PDRG1 gene from black tiger shrimp (Penaeus monodon)
Provedor de dados: Genetics and Molecular Biology
Autores: Zhao,Chao; Dai,Wenting; Qiu,Lihua.
Abstract P53 And DNA Damage-Regulated Gene 1 (PDRG1) is a novel gene which plays an important role in chaperone-mediated protein folding. In the present study, the full-length complementary DNA (cDNA) sequence of the PDRG1 gene from Penaeus monodon (PmPDRG1) was cloned by the rapid amplification of cDNA ends (RACE) method. The cDNA of PmPDRG1 spans 1,613 bp, interrupted by only one short intron, and encodes a protein of 136 amino acids with calculated molecular weight of 15.49 kDa. The temporal expression profile of PmPDRG1 in different tissues and in different developmental stages of the ovary was investigated by real-time quantitative PCR (RT-qPCR). An RNA interference (RNAi) experiment was performed to study the relationship between P. monodon p53...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PDRG1; Gene cloning; RT-qPCR; Ovarian development; Black tiger shrimp.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100093

Association of a serotonin transporter gene (SLC6A4) 5-HTTLPR polymorphism with body mass index categories but not type 2 diabetes mellitus in Mexicans
Provedor de dados: Genetics and Molecular Biology
Autores: Peralta-Leal,Valeria; Leal-Ugarte,Evelia; Meza-Espinoza,Juan P.; Dávalos-Rodríguez,Ingrid P.; Bocanegra-Alonso,Anabel; Acosta-González,Rosa I.; Gonzales,Enrique; Nair,Saraswathy; Durán-González,Jorge.
The serotonergic system has been hypothesized to contribute to the biological susceptibility to type 2 diabetes mellitus (T2DM) and body-mass index (BMI) categories. We investigate a possible association of 5-HTTLPR polymorphism (L and S alleles) in the promoter region of the serotonin transporter gene (SLC6A4) with the development of T2DM and/or higher BMI by analyzing a sample of 138 individuals diagnosed with T2DM and 172 unrelated controls from the Mexican general population. In the total sample genotypes were distributed according to Hardy- Weinberg equilibrium, and S allele frequency was 0.58. There was no statistical association between 5-HTTLPR polymorphism and the development of T2DM in this Mexican population sample (p = 0.12). Nevertheless,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SLC6A4 gene; 5-HTTLPR polymorphism; Type 2 diabetes mellitus; Body mass index.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400007

The complete mitochondrial genome of the threatened Neotropical catfish Lophiosilurus alexandri (Silurifomes: Pseudopimelodidae) and phylogenomic analysis indicate monophyly of Pimelodoidea
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Daniel Cardoso; Perini,Violeta da Rocha; Bastos,Alex Schomaker; Costa,Igor Rodrigues da; Luz,Ronald Kennedy; Furtado,Carolina; Prosdocimi,Francisco.
Abstract Lophiosilurus alexandri is an endemic catfish from the São Francisco River Basin (Brazil) popularly known as pacamã, which has economic potential for aquaculture farming. The mitochondrial genome was sequenced for the threatened Neotropical catfish L. alexandri. Assembly into scaffolds using MIRA and MITObim software produced the whole, circularized mitochondrial genome, which comprises 16,445 bp and presents the typical gene arrangement of Teleostei mitochondria. A phylogenomic analysis was performed after the concatenation of all proteins obtained from whole mitogenomes of 20 Siluriformes and two outgroups. The results confirmed the monophyly of nine families of catfishes and also clustered L. alexandri as a sister group to the family...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mitogenome; Fish; Next-generation-sequencing; Illumina; Pseudopimelodidae.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400674

A genomic analysis of disease-resistance genes encoding nucleotide binding sites in Sorghum bicolor
Provedor de dados: Genetics and Molecular Biology
Autores: Cheng,Xiao; Jiang,Haiyang; Zhao,Yang; Qian,Yexiong; Zhu,Suwen; Cheng,Beijiu.
A large set of candidate nucleotide-binding site (NBS)-encoding genes related to disease resistance was identified in the sorghum (Sorghum bicolor) genome. These resistance (R) genes were characterized based on their structural diversity, physical chromosomal location and phylogenetic relationships. Based on their N-terminal motifs and leucine-rich repeats (LRR), 50 non-regular NBS genes and 224 regular NBS genes were identified in 274 candidate NBS genes. The regular NBS genes were classified into ten types: CNL, CN, CNLX, CNX, CNXL, CXN, NX, N, NL and NLX. The vast majority (97%) of NBS genes occurred in gene clusters, indicating extensive gene duplication in the evolution of S. bicolor NBS genes. Analysis of the S. bicolor NBS phylogenetic tree revealed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics; Disease resistance gene; Nucleotide binding site; Sorghum bicolor.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200016

Genetic differentiation in geographically close populations of the water rat Nectomys squamipes (Rodentia, Sigmodontinae) from the Brazilian Atlantic Forest
Provedor de dados: Genetics and Molecular Biology
Autores: Maroja,L.S.; Almeida,F.C.; Seuánez,H.N.
We examined the genetic structure and the effects of a bottleneck in populations of the water rat Nectomys squamipes, a primary host of Schistosoma mansoni. Eight microsatellite loci were studied in 7 populations from the Sumidouro region of the Brazilian state of Rio de Janeiro. Our data, covering a four-year period during which a bottleneck occurred, revealed substantial variation (6-31 alleles per locus) and high levels of both observed (0.718-0.789) and expected (0.748-0.832) heterozygosity. Most populations were in Hardy-Weinberg equilibrium without linkage disequilibrium between loci. Overall average genetic differentiation between populations (estimated with the F ST (q) and R ST (r) analogues was 0.037 for q and 0.060 for r. There was significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Migration; Population genetics; Schistosoma mansoni host; Sigmodontinae.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400002

Impact of co-blocking the costimulatory signals on immune-related genes after high-risk rabbit corneal allograft using 2nd-generation DNA sequencing technology
Provedor de dados: Genetics and Molecular Biology
Autores: Zhao,Hai-Xia; Li,Xin-Yu; Guan,Wen-Ying; Han,Xiao-Tong.
Abstract The aim of this study was to evaluate the impact and mechanism of co-blocking of costimulatory signals CD28-B7-CD40-CD40L during immune allograft rejection. Forty-eight recipient rabbits were prepared as a high-risk corneal allograft model. After surgery, the animals were randomly divided into: control group, MR1 group, anti-B7 group, and co-blocking group (n=12, each group). Subconjunctival injection was first performed on the allograft surgery day until post-surgery day five. Four weeks later, or when immune rejection occurred, the cornea was sampled to detect and analyze the gene spectrum. The survival time in the co-blocking group was significantly longer than that in the other three groups (p &lt; 0.05). Gene expression analysis revealed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Costimulatory signal; Corneal allograft; Immune rejection; Second-generation sequencing technology; Antibody.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300472

The genetic base of Brazilian soybean cultivars: evolution over time and breeding implications
Provedor de dados: Genetics and Molecular Biology
Autores: Wysmierski,Philip Traldi; Vello,Natal Antonio.
Genetic diversity is essential for crop breeding and one way to estimate it is through the concept of genetic base, which can be defined as the number of ancestors and their relative genetic contributions (RGC) to each cultivar. The RGC can be estimated through the coefficient of parentage between the ancestors and cultivars. Previous studies determined that the genetic base of Brazilian soybean was very narrow. The objective of this work was to evaluate the pedigree of 444 Brazilian soybean cultivars to estimate their genetic base. The cultivars were divided according to their release dates and according to their origin (public or private), and the genetic base for each group was also estimated. We found 60 ancestors, of which the top four (CNS, S-100,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic base; Glycine max; Coefficient of parentage; Genetic vulnerability.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400014

Demography, genetic diversity, and population relationships among Argentinean Mapuche Indians
Provedor de dados: Genetics and Molecular Biology
Autores: Goicoechea,Alicia S.; Carnese,Francisco R.; Caratini,Alicia L.; Avena,Sergio; Salaberry,Maria; Salzano,Francisco M.
Fertility, mortality and migration data from four Mapuche Indian communities located along a 215-km NE-SW linear area in the Province of Río Negro, Argentina, were collated with genetic information furnished by nine blood group systems and by mtDNA haplogroups. The demographic and genetic data indicated a clear dichotomy, which split the four populations into two groups of two. Differing degrees of non-Indian exchanges was probably the main determining factor for this separation. Total genetic variability was very similar in all groups, and the interpopulational variability accounted for only 10% of the total variability. A low prevalence of the Diego(a) antigen among the Mapuche was confirmed. The fact that significant genetic heterogeneity and population...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300001

Stages in the evolution of the Hardy-Weinberg law
Provedor de dados: Genetics and Molecular Biology
Autores: Stark,Alan E.
The Hardy-Weinberg law has been used widely for about one hundred years with little question as to the foundations laid down by its originators. The basic assumption of random mating, that is choice of mates by a process akin to that of a lottery, was shown to produce genotypic proportions following the "binomial-square" rule, the so-called Hardy-Weinberg proportions (HWP). It has been assumed by many that random mating was the only way of pairing genes capable of producing HWP. However it has been shown that HWP can be obtained and maintained by non-random mating. The steps along the way to this revelation and some implications are reviewed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hardy-Weinberg law; Non-random mating.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400001

Determining mutations in G6PC and SLC37A4 genes in a sample of Brazilian patients with glycogen storage disease types Ia and Ib
Provedor de dados: Genetics and Molecular Biology
Autores: Carlin,Marcelo Paschoalete; Scherrer,Daniel Zanetti; Tommaso,Adriana Maria Alves De; Bertuzzo,Carmen Silvia; Steiner,Carlos Eduardo.
Glycogen storage disease (GSD) comprises a group of autosomal recessive disorders characterized by deficiency of the enzymes that regulate the synthesis or degradation of glycogen. Types Ia and Ib are the most prevalent; while the former is caused by deficiency of glucose-6-phosphatase (G6Pase), the latter is associated with impaired glucose-6-phosphate transporter, where the catalytic unit of G6Pase is located. Over 85 mutations have been reported since the cloning of G6PC and SLC37A4 genes. In this study, twelve unrelated patients with clinical symptoms suggestive of GSDIa and Ib were investigated by using genetic sequencing of G6PC and SLC37A4 genes, being three confirmed as having GSD Ia, and two with GSD Ib. In seven of these patients no mutations...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: DNA-based diagnosis; Glycogen storage disease; G6PC; SLC37A4; Mutation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400007

MHC Class II haplotypes of Colombian Amerindian tribes
Provedor de dados: Genetics and Molecular Biology
Autores: Yunis,Juan J.; Yunis,Edmond J.; Yunis,Emilio.
We analyzed 1041 individuals belonging to 17 Amerindian tribes of Colombia, Chimila, Bari and Tunebo (Chibcha linguistic family), Embera, Waunana (Choco linguistic family), Puinave and Nukak (Maku-Puinave linguistic families), Cubeo, Guanano, Tucano, Desano and Piratapuyo (Tukano linguistic family), Guahibo and Guayabero (Guayabero Linguistic Family), Curripaco and Piapoco (Arawak linguistic family) and Yucpa (Karib linguistic family). for MHC class II haplotypes (HLA-DRB1, DQA1, DQB1). Approximately 90% of the MHC class II haplotypes found among these tribes are haplotypes frequently encountered in other Amerindian tribes. Nonetheless, striking differences were observed among Chibcha and non-Chibcha speaking tribes. The DRB1*04:04, DRB1*04:11, DRB1*09:01...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MHC; Class II; Amerindians; Colombia; Linguistic.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200004

Family number in common bean selection
Provedor de dados: Genetics and Molecular Biology
Autores: Ferreira,Wilton Duarte; Ramalho,Magno Antonio Patto; Ferreira,Daniel Furtado; Souza,Moacil Alves de.
The objective of the present study was to determine the number of families necessary for selection in a segregant population. Nine segregant populations from the bean improvement program of the Federal University of Lavras were evaluated. The number of families varied from 169 to 295, and the average family heritability estimate (h²) for grain production varied from 5.3 to 82.0%. Different sample sizes were simulated using the average family performance. The first sample consisted of 30 families, with each additional sample containing 10 more families, until the total number of families evaluated was reached for each population. One thousand simulations per sample size were performed. These data were used to determine the average, minimum and maximum...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200028

Presence of two mitochondrial genomes in the mytilid Perumytilus purpuratus: Phylogenetic evidence for doubly uniparental inheritance
Provedor de dados: Genetics and Molecular Biology
Autores: Vargas,Jaime; Pérez,Montse; Toro,Jorge; Astorga,Marcela P.
This study presents evidence, using sequences of ribosomal 16S and COI mtDNA, for the presence of two mitochondrial genomes in Perumytilus purpuratus. This may be considered evidence of doubly uniparental mtDNA inheritance. The presence of the two types of mitochondrial genomes differentiates females from males. The F genome was found in the somatic and gonadal tissues of females and in the somatic tissues of males; the M genome was found in the gonads and mantle of males only. For the mitochondrial 16S region, ten haplotypes were found for the F genome (nucleotide diversity 0.004), and 7 haplotypes for the M genome (nucleotide diversity 0.001), with a distance Dxy of 0.125 and divergence Kxy of 60.33%. For the COI gene 17 haplotypes were found for the F...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mytilidae; 16S; COI; Perumytilus; DUI.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200173

Soft inheritance: challenging the modern synthesis
Provedor de dados: Genetics and Molecular Biology
Autores: Jablonka,Eva; Lamb,Marion J.
This paper presents some of the recent challenges to the Modern Synthesis of evolutionary theory, which has dominated evolutionary thinking for the last sixty years. The focus of the paper is the challenge of soft inheritance - the idea that variations that arise during development can be inherited. There is ample evidence showing that phenotypic variations that are independent of variations in DNA sequence, and targeted DNA changes that are guided by epigenetic control systems, are important sources of hereditary variation, and hence can contribute to evolutionary changes. Furthermore, under certain conditions, the mechanisms underlying epigenetic inheritance can also lead to saltational changes that reorganize the epigenome. These discoveries are clearly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epigenetic inheritance; Hereditary variation; Lamarckism; Macroevolution; Microevolution.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300001

Caffeine content of Ethiopian Coffea arabica beans
Provedor de dados: Genetics and Molecular Biology
Autores: Silvarolla,Maria Bernadete; Mazzafera,Paulo; Lima,Marinez Muraro Alves de.
The coffee germplasm bank of the Instituto Agronômico de Campinas has many Coffea arabica accessions from Ethiopia, which is considered the primary center of genetic diversity in coffee plants. An evaluation of the caffeine content of beans from 99 progenies revealed intra- and inter-progeny variability. In 68 progenies from the Kaffa region we found caffeine values in the range 0.46-2.82% (mean 1.18%), and in 22 progenies from Illubabor region these values ranged from 0.42 to 2.90% (mean 1.10%). This variability could be exploited in a breeding program aimed at producing beans with low-caffeine content.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100036

Human and medical genetics in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Beiguelman,Bernardo.
The present paper intends to be a brief historical analysis of the conditions for the development of Human and Medical Genetics in Brazil.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200006

Genetic frequencies related to severe or profound sensorineural hearing loss in Inner Mongolia Autonomous Region
Provedor de dados: Genetics and Molecular Biology
Autores: Liu,Yongzhi; Ao,Liying; Ding,Haitao; Zhang,Dongli.
Abstract The aim was to study the frequencies of common deafness-related mutations and their contribution to hearing loss in different regions of Inner Mongolia. A total of 738 deaf children were recruited from five different ethnic groups of Inner Mongolia, including Han Chinese (n=486), Mongolian (n=216), Manchurian (n=24), Hui (n=6) and Daur (n=6). Nine common mutations in four genes (GJB2, SLC26A4, GJB3 and mitochondrial MT-RNR1 gene) were detected by allele-specific PCR and universal array. At least one mutated allele was detected in 282 patients. Pathogenic mutations were detected in 168 patients: 114 were homozygotes and 54 were compound heterozygotes. The 114 patients were carriers of only one mutated allele. The frequency of GJB2 variants in Han...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sensorineural hearing loss; GJB2; SLC26A4; GJB3; Mitochondrial DNA; Inner Mongolia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400567

Screening for germline BRCA1, BRCA2, TP53 and CHEK2 mutations in families at-risk for hereditary breast cancer identified in a population-based study from Southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Palmero,Edenir Inêz; Alemar,Bárbara; Schüler-Faccini,Lavínia; Hainaut,Pierre; Moreira-Filho,Carlos Alberto; Ewald,Ingrid Petroni; Santos,Patricia Koehler dos; Ribeiro,Patricia Lisbôa Izetti; Oliveira Netto,Cristina Brinkmann de; Calvez-Kelm,Florence Le; Tavtigian,Sean; Cossio,Silvia Liliana; Giugliani,Roberto; Caleffi,Maira; Ashton-Prolla,Patricia.
Abstract In Brazil, breast cancer is a public health care problem due to its high incidence and mortality rates. In this study, we investigated the prevalence of hereditary breast cancer syndromes (HBCS) in a population-based cohort in Brazils southernmost capital, Porto Alegre. All participants answered a questionnaire about family history (FH) of breast, ovarian and colorectal cancer and those with a positive FH were invited for genetic cancer risk assessment (GCRA). If pedigree analysis was suggestive of HBCS, genetic testing of the BRCA1, BRCA2, TP53, and CHEK2 genes was offered. Of 902 women submitted to GCRA, 214 had pedigrees suggestive of HBCS. Fifty of them underwent genetic testing: 18 and 40 for BRCA1/BRCA2 and TP53 mutation screening,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer predisposition syndrome; Hereditary breast cancer; Genetic cancer risk assessment.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200210

Modulation of thyroid hormone receptor transactivation by the early region 1A (E1A)-like inhibitor of differentiation 1 (EID1)
Provedor de dados: Genetics and Molecular Biology
Autores: Vargas,Diana; Celis,Luis; Romero,Carolina; Lizcano,Fernando.
Transcriptional activation (TA) mediated by the effect of thyroid hormones on target genes requires co-activator proteins such as the early region 1A (E1A) associated 300 kDa binding protein (p300) and the cAMP response element binding protein (CREB) binding protein (CBP), known as the p300/CBP complex, which acetylate histones 3 and 4 to allow transcriptional machinery access to the target gene promoter. Little is known on the role of p300 in thyroid hormone receptor (TR) mediated TA but the E1A-like inhibitor of differentiation 1 (EID1), an inhibitor of p300 histone acetyltransferase (HAT), is a functional homolog of E1A and may inhibit myogenic differentiation factor D (MyoD) transcriptional activity and reduces muscle cell differentiation. We evaluated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Co-activator; EID1; P300; Thyroid receptors; Transcription.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300003

CRZ1 regulator and calcium cooperatively modulate holocellulases gene expression in Trichoderma reesei QM6a
Provedor de dados: Genetics and Molecular Biology
Autores: Martins-Santana,Leonardo; Paula,Renato Graciano de; Silva,Adriano Gomes; Lopes,Douglas Christian Borges; Silva,Roberto do Nascimento; Silva-Rocha,Rafael.
Abstract Trichoderma reesei is the main filamentous fungus used in industry to produce cellulases. Here we investigated the role of CRZ1 and Ca2+signaling in the fungus T. reesei QM6a concerning holocellulases production. For this, we first searched for potential CRZ1 binding sites in promoter regions of key genes coding holocellulases, as well as transcriptional regulators and sugar and calcium transporters. Using a nearly constructed T. reeseiAcrz1 strain, we demonstrated that most of the genes expected to be regulated by CRZ1 were affected in the mutant strain induced with sugarcane bagasse (SCB) and cellulose. In particular, our data demonstrate that Ca2+ acts synergistically with CRZ1 to modulate gene expression, but also exerts CRZ1-independent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene regulatory network; Cellulase expression; Transcriptional regulation; Calcium sensing.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400401

Bleomycin sensitivity in patients with familial and sporadic polyposis: a pilot study
Provedor de dados: Genetics and Molecular Biology
Autores: Sales,Magaly M.; Lucca,Edmundo J. de; Yamashita,Seizo; Saad,Luis Henrique Cury.
Human peripheral blood lymphocytes from 10 patients with familial adenomatous polyposis (FAP) showed a significantly higher incidence of chromatid breaks when compared to cells from 10 normal individuals, after exposure to bleomycin (BLM) during the G2 phase. However, no significant increase in bleomycin sensitivity was observed in lymphocytes from 10 patients with sporadic adenomatous polyps (AP) vs. 10 normal individuals (P = 0.67). Individuals that exhibited an average number of chromatid breaks per cell higher than 0.80 were considered sensitive to the drug. No control showed susceptibility to BLM, as compared to 3 out of 20 patients.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100004

Exploiting a wheat EST database to assess genetic diversity
Provedor de dados: Genetics and Molecular Biology
Autores: Karakas,Ozge; Gurel,Filiz; Uncuoglu,Ahu Altinkut.
Expressed sequence tag (EST) markers have been used to assess variety and genetic diversity in wheat (Triticum aestivum). In this study, 1549 ESTs from wheat infested with yellow rust were used to examine the genetic diversity of six susceptible and resistant wheat cultivars. The aim of using these cultivars was to improve the competitiveness of public wheat breeding programs through the intensive use of modern, particularly marker-assisted, selection technologies. The F2 individuals derived from cultivar crosses were screened for resistance to yellow rust at the seedling stage in greenhouses and adult stage in the field to identify DNA markers genetically linked to resistance. Five hundred and sixty ESTs were assembled into 136 contigs and 989 singletons....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity; EST; Genetic diversity; Triticum; Yellow rust.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400019

Chromosome study in Schistocerca (Orthoptera-Acrididae-Cyrtacanthacridinae): karyotypes and distribution patterns of constitutive heterochromatin and nucleolus organizer regions (NORs)
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Maria José de; Melo,Natoniel Franklin de.
Chromosome analyses were performed in two grasshopper species of the genus Schistocerca, S. pallens and S. flavofasciata. Both species shared the same diploid number (2n = 23, X in males; 2n = 24, XX in females);and a conserved karyotype composed exclusively of acrocentric chromosomes, but differed in their distribution patterns of constitutive heterochromatin and nucleolus organizer regions (NORs). Constitutive heterochromatin was located in the pericentromeric region of all chromosomes in both species. S. flavofasciata presented an additional C-band on the distal region of the long arm of a small autosome pair (S9). Nucleolus organizer regions (NORs), revealed by silver nitrate staining (Ag-NORs), were observed on a medium autosome pair (M5) in both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grasshopper; Constitutive heterochromatin; Nucleolus organizer regions.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100011

The perturbed expression of m6A in parthenogenetic mouse embryos
Provedor de dados: Genetics and Molecular Biology
Autores: Hao,Jindong; Xianfeng,Yu; Gao,Wei; Wei,Jiaqi; Qi,Minghui; Han,Liang; Shi,Shuming; Lin,Chao; Wang,Dongxu.
Abstract Parthenogenetically activated oocytes cannot develop to term in mammals owing to abnormal epigenetic modifications. Methylation of the N6 position of adenosine (m6A) is a post-transcriptional epigenetic modification of RNA. To investigate the role of m6A methylation in parthenogenetic (PA) embryonic development, we analyzed METTL3, METTL14, FTO, ALKBH5, YTHDF2, IGF2BP1, and IGF2BP2 expression by quantitative real-time PCR. These genes were found dynamically expressed during the 2-cell, 4-cell, 8-cell, and blastocyst stages of the embryo. Compared to normally fertilized embryos, the expression of these genes was perturbed in PA embryos, especially at the 8-cell stage. Furthermore, immunofluorescence was used to detect m6A expression. The results...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: M6A; Gene expression; Embryo development; Parthenogenetic.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400666

Complete mitochondrial genome of the Florida manatee (Trichechus manatus latirotris, Sirenia)
Provedor de dados: Genetics and Molecular Biology
Autores: Vilaça,Sibelle T.; Santos,Fabricio R.
Abstract The Florida manatee (Trichechus manatus latirostris) is an endangered subspecies of the West Indian manatee (T. manatus), which inhabits inland and marine waters of southeastern United States. In this study, we assembled the mitochondrial genome (mtDNA) of the Florida manatee from whole genome shotgun reads. As a result, we show that the currently annotated T. manatus mtDNA belongs to a different species, the Amazonian manatee (T. inunguis). The newly assembled Florida manatee mtDNA is 16,881 bp in length, with 13 protein-coding genes, two ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs) and one non-coding control region (D-loop). Phylogenetic analysis based on the control region indicates the newly assembled mtDNA is haplotype A01, characteristic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichechus manatus; Florida manatee; Amazonian manatee; Mitochondrial genome.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500201

Microsatellite variability analysis in farmed catfish (Ictalurus punctatus) from Tamaulipas, Mexico
Provedor de dados: Genetics and Molecular Biology
Autores: Perales-Flores,Laura E.; Sifuentes-Rincón,Ana María; León,Francisco J. García de.
Analysis of cultured catfish from six farms in Tamaulipas, Mexico was achieved using a combination of microsatellite PCR analysis and semiautomatic fluoresce-based detection, in order to provide a first assessment of the genetic variability on cultured catfish in Mexico. Five microsatellites showed extensive polymorphism with allele numbers ranging from 10 and 20. Overall observed heterozygosity at each locus ranged between 0.76 and 0.91 and the average polymorphic information content (PIC) for the five loci was 0.811, indicating that these loci can be used for studies of paternity identification, linkage and population genetics. On the basis of the F ST values (F ST = 0.03829; p = 0.00000) it appears that there was a small amount of genetic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ictaluridae; Molecular characterization; Genetic variability.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400011

Cloning and expression of embryogenesis-regulating genes in Araucaria angustifolia (Bert.) O. Kuntze (Brazilian Pine)
Provedor de dados: Genetics and Molecular Biology
Autores: Schlögl,Paulo Sérgio; Santos,André Luis Wendt dos; Vieira,Leila do Nascimento; Floh,Eny Iochevet Segal; Guerra,Miguel Pedro.
Angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago. Apart from morphological and structural differences in embryogenesis and seed origin, a set of embryogenesis-regulating genes and the molecular mechanisms involved in embryo development seem to have been conserved alike in both taxa. Few studies have covered molecular aspects of embryogenesis in the Brazilian pine, the only economically important native conifer in Brazil. Thus eight embryogenesis-regulating genes, viz.,ARGONAUTE 1, CUP-SHAPED COTYLEDON 1, WUSCHEL-related WOX, S-LOCUS LECTIN PROTEIN KINASE, SCARECROW-like, VICILIN 7S, LEAFY COTYLEDON 1, and REVERSIBLE GLYCOSYLATED POLYPEPTIDE 1, were analyzed through semiquantitative RT-PCR during embryo development...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seed development; Brazilian Pine; Embryogenesis-regulating genes; Zygotic embryogenesis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100025

Natural cross-pollination in roselle, Hibiscus sabdariffa L. (Malvaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Vaidya,K.R.
Two local varieties of roselle (Hibiscus sabdariffa L.), Jamaican Green and Jamaican Red, were grown to determine the amount of natural cross-pollination. Two planting arrangements (alternating rows; alternating individuals in a row) and two planting dates, a month apart, were used for the outcrossing experiments. Stem pigmentation, red (R-) vs. green (rr), was used as a genetic marker in the estimation of outcrossing. Homozygous dominant and recessive genotypes of Jamaican Red and Jamaican Green, respectively, were grown in both of the planting arrangements and dates. Seeds from open-pollinated capsules of randomly selected Jamaican Green plants were planted to score the frequency of cross-pollination. Estimates of natural cross-pollination ranged from...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300027

Molecular biogeography of the Neotropical fish Hoplias malabaricus (Erythrinidae:Characiformes)  in the Iguaçu, Tibagi, and Paraná Rivers
Provedor de dados: Genetics and Molecular Biology
Autores: Dergam,Jorge A.; Suzuki,Harumi Irene; Shibatta,Oscar A.; Duboc,Luiz F.; Júlio Jr,Horácio F.; Giuliano-Caetano,Lúcia; Black IV,William C.
Hoplias malabaricus, the common trahira, extended its range into the Iguaçu River sometime in the last decades. To determine levels of genetic differentiation in neighboring basins of this nominal taxon, as well as to unveil the source of the invading population, we analyzed patterns of similarity of RAPD-PCR genomic markers of samples from Paraná, Iguaçu and Tibagi Rivers. The high genetic diversity of samples from the Paraná and Tibagi Rivers suggested strong population structuring or even the occurrence of undescribed species. All alleles of the sample from the headwaters of Tibagi River were present in the sample from the Iguaçu River, which suggests that the former population or another population with similar genetic makeup, may be the source of the...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400015

Genetic variance and covariance components  related to intra- and interpopulation  recurrent selection in maize (Zea mays L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Arias,Carlos Alberto Arrabal; Souza Júnior,Cláudio Lopes de.
New genetic variance and covariance components related to intra- and interpopulational recurrent selection methods have been theoretically developed by Souza Jr. (Rev. Bras. Genet. 16: 91-105, 1993) to explain the failure of these methods to concomitantly develop hybrid and per se populations. Intra- and interpopulation half-sib progenies of 100 genotypes were sampled from maize (Zea mays L.) populations BR-106 and BR-105 to estimate variance and covariance components and to compare the expected responses to reciprocal (RRS), intrapopulational (HSS), and modified (MRS) recurrent selection in interpopulation hybrid, populations per se, and to determine heterosis. Four sets of 100 progenies, two intra- and two interpopulational, were evaluated in partially...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400022

Cytogenetic and molecular contributions to the study of mental retardation
Provedor de dados: Genetics and Molecular Biology
Autores: Llerena Jr.,Juan C.; Almeida,José Carlos Cabral de.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200018

Genetics (not only) for dummies
Provedor de dados: Genetics and Molecular Biology
Autores: Cipolla,Gabriel Adelman; Beltrame,Marcia Holsbach.
Tipo: Info:eu-repo/semantics/review
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300550

Differential detection of transposable elements between Saccharum species
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Marislane Carvalho Paz de; Silva,Jéssica Naiana; Almeida,Cícero.
Cultivars of sugarcane (Saccharum) are hybrids between species S. officinarum (x = 10, 2n = 8x = 80) and S. spontaneum (x=8,2n=5-16x =40-128). These accessions have 100 to 130 chromosomes, 80-85% of which are derived from S. officinarum, 10-15% from S. spontaneum, and 5-10% are possible recombinants between the two genomes. The aim of this study was to analyze the repetition of DNA sequences in S. officinarum and S. spontaneum. For this purpose, genomic DNA from S. officinarum was digested with restriction enzymes and the fragments cloned. Sixty-eight fragments, approximately 500 bp, were cloned, sequenced and had their identity analyzed in NCBI, and in the rice, maize, and sorghum genome databases using BLAST. Twelve clones containing partial transposable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sugarcane; Dot-blot hybridization; LTR retrotransposons; Repetitive DNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300016

Path analysis suggests phytoene accumulation is the key step limiting the carotenoid pathway in white carrot roots
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Carlos Antonio Fernandes; Senalik,Douglas; Simon,Philipp Wallace.
Two F2 carrot (Daucus carota L.) populations (orange rooted Brasilia x very dark orange rooted High Carotene Mass - HCM cross and the dark orange rooted cultivated variety B493 x white rooted wild carrot Queen Anne's Lace - QAL cross) with very unrelated genetic backgrounds were used to investigate intrinsic factors limiting carotenoid accumulation in carrots by applying phenotypic correlation and path analysis to study the relationships between major root carotenes, root color and several other morphological traits. Most of the correlations between traits were close and agreed in sign between the two populations. Root weight had a moderate to highly significant positive correlation with leaf length, root length and top and middle root diameter. Although...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Daucus carota; Correlation; Path diagram; Genetic engineering.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200019

RUNX2 mutations in Taiwanese patients with cleidocranial dysplasia
Provedor de dados: Genetics and Molecular Biology
Autores: Lin,Wei-De; Lin,Shuan-Pei; Wang,Chung-Hsing; Tsai,Yushin; Chen,Chih-Ping; Tsai,Fuu-Jen.
Cleidocranial dysplasia (CCD) is an autosomal dominant human skeletal disorder comprising hypoplastic clavicles, wide cranial sutures, supernumerary teeth, short stature, and other skeletal abnormalities. It is known that mutations in the human RUNX2 gene mapped at 6p21 are responsible for CCD. We analyzed the mutation patterns of the RUNX2 gene by direct sequencing in six Taiwanese index cases with typical CCD. One of the patients was a familial case and the others were sporadic cases. Sequencing identified four mutations. Three were caused by single nucleotide substitutions, which created a nonsense (p.R391X), two were missense mutations (p.R190W, p.R225Q), and the forth was a novel mutation (c.1119delC), a one-base deletion. Real time quantitative PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cleidocranial dysplasia; CCD; RUNX2; RUNX2 deletion mutation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200005

The SCA1 (Spinocerebellar ataxia type 1) and MJD (Machado-Joseph disease) CAG repeats in normal individuals: segregation analysis and allele frequencies
Provedor de dados: Genetics and Molecular Biology
Autores: Wiezel,Cláudia Emília Vieira; Canas,Maria do Carmo Tomitão; Simões,Aguinaldo Luiz.
Spinocerebellar ataxia type 1 (SCA1) and Machado-Joseph disease (MJD/SCA3) are autosomal dominant neurodegenerative diseases caused by expansions of a CAG trinucleotide repeat in the SCA1 and MJD genes. These expanded sequences are unstable upon transmission, leading to an intergeneration increase in the number of repeats (dynamic mutation). The transmission of the CAG repeat was studied in normal mother-father-child trios, referred for paternity testing (SCA1, n = 367; MJD, n = 879). No segregation distortion was detected. The CAG allele frequencies were determined in 330 unrelated individuals (fathers from couples tested for paternity). The allele frequency distributions did not differ from those previously reported for European populations. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Segregation distortion; Spinocerebellar ataxia type 1; Machado-Joseph disease.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200002

Cytogenetic characterization of a population of Bryconamericus aff. iheringii (Characidae,Tetragonopterinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Paintner-Marques,Tânia Regina; Giuliano-Caetano,Lucia; Dias,Ana Lúcia.
A cytogenetic analysis of thirteen specimens of Bryconamericus aff. Iheringii revealed a diploid number of 52 chromosomes and a karyotype of 8M+22SM+10ST+12A with a fundamental number (FN) of 92. The nucleolus organizer regions (NORs) were studied by means of AgNO3 and CMA3 staining as well as by FISH using an 18S rDNA probe. NORs were found to be located at the terminal position on the short arm of the submetacentric chromosome pair. C-banding showed strong telomeric and centromeric staining in the majority of the chromosomes, and a similar pattern was observed after treatment with AluI restriction enzyme
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AgNOR; Alu I; Bryconamericus; C-banding; CMA3; RDNA 18S.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200007

Genetic characterization of the Spanish Trotter horse breed using microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Javier Azor,Pedro; Valera,Mercedes; Gómez,María Dolores; Goyache,Félix; Molina,Antonio.
To assist in selection schemes we carried out the first genetic characterization of the Spanish Trotter horse (Trotador Español). We used 16 microsatellite markers to genotype 40 unrelated Spanish trotters, 25 native Balearic horses (11 Menorquina and 14 Mallorquina horses) and 32 Andalusian horses. The observed heterozygosity for the Spanish Trotters was 0.647 ± 0.037 and the expected heterozygosity was 0.696 ± 0.026 while the average number of alleles per locus was 6.0 ± 0.341, these values being similar to the data published for other horse breeds. We also tried to establish the importance of the Mallorquina and Menorquina breeds in the present Spanish Trotter population. Only 9% of the total genetic variability could be attributed to differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse; Spanish Trotter breed; Microsatellites; Genetic diferenciation; Migration rates.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100009

Genetic effects for maize traits in acid and non-acid soils
Provedor de dados: Genetics and Molecular Biology
Autores: Pérez Velásquez,Juan C.; Souza Jr.,Cláudio Lopes de; Narro,Luis A.; Pandey,Shivaji; De León,Carlos.
Breeding programs for acid-soil tolerance are desirable as a relatively inexpensive and permanent way for increasing maize (Zea mays L.) yield on these soils. Our objective was to compare the genetic effects controlling the expression of maize traits in acid and non-acid soils. Seven related and one unrelated inbred lines, with different levels of tolerance to acid soil, and their F1, F2, BC1, and BC2 generations were evaluated in four acid and two non-acid soils. Estimates of additive, dominance, and epistatic effects were computed for grain yield, plant height, days to mid-silk, and prolificacy, using the generation means analysis procedure. For all traits the major part of the variation was accounted for by additive and dominance effects, with dominance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acid soils; Additive; Dominant; Epistatic effects; Generation analysis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100017

Editorial
Provedor de dados: Genetics and Molecular Biology
Autores: Freitas,Loreta B.
Tipo: Info:eu-repo/semantics/other
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600001

An effective device for generating alginate microcapsules
Provedor de dados: Genetics and Molecular Biology
Autores: Bressel,Tatiana A.B.; Paz,Ana Helena; Baldo,Guilherme; Lima,Elizabeth O. Cirne; Matte,Ursula; Saraiva-Pereira,Maria Luiza.
An alternative approach to somatic gene therapy is to deliver the therapeutic protein by implanting genetically modified cells that could overexpress the gene of interest. Microencapsulation devices were designed to protect cells from host rejection and prevent the foreign cells from spreading while allowing protein secretion. Alginate microcapsules form a semi-permeable structure that is suitable for in vivo injection. In this study, we report an effective laboratory protocol for producing calcium alginate microcapsules, following optimization of uniformly shaped and sized particles containing viable cells. Encapsulation of baby hamster kidney (BHK) cells in alginate microcapsules was performed using a simple device consisting of a cylinder of compressed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alginate; Beads controlled release drug delivery microcapsules.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100023

Centric fusion in goats (Capra hircus): Identification of a 6/15 translocation by high resolution chromosome banding
Provedor de dados: Genetics and Molecular Biology
Autores: Mota,Lígia Souza Lima Silveira da; Silva,Rosana Ap. Bicudo da.
Nine phenotypically normal Saanen goats (6 males and 3 females) belonging to the goat house of the Instituto de Biociências, UNESP, Botucatu Campus, were analyzed cytogenetically. The objective of the present study was to determine whether or not chromosomes 5 and 15 are involved in a Robertsonian translocation previously observed in a sample of a Brazilian herd using the chromosome pattern described by the ISCNDA (1989). The results suggest the involvement of chromosomes 6 and 15 in the fusion demonstrated by G-banding in prometaphase cells. The Brazilian sample of animals carrying structural rearrangements did not present any reduction in fertility, suggesting the existence of prezygotic selection against unbalanced gametes. Further investigations on the...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100012

Linkage analysis between dominant and co-dominant makers in full-sib families of out-breeding species
Provedor de dados: Genetics and Molecular Biology
Autores: Alves,Alexandre Alonso; Bhering,Leonardo Lopes; Cruz,Cosme Damião; Alfenas,Acelino Couto.
As high-throughput genomic tools, such as the DNA microarray platform, have lead to the development of novel genotyping procedures, such as Diversity Arrays Technology (DArT) and Single Nucleotide Polymorphisms (SNPs), it is likely that, in the future, high density linkage maps will be constructed from both dominant and co-dominant markers. Recently, a strictly genetic approach was described for estimating recombination frequency (r) between co-dominant markers in full-sib families. The complete set of maximum likelihood estimators for r in full-sib families was almost obtained, but unfortunately, one particular configuration involving dominant markers, segregating in a 3:1 ratio and co-dominant markers, was not considered. Here we add nine further...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Statistical genomics; Exogamic populations; Recombination frequency and maximum likelihood.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300021

Identification of a rare p.G320R alpha-1-antitrypsin variant in emphysema and lung cancer patients
Provedor de dados: Genetics and Molecular Biology
Autores: Ljujic,Mila; Topic,Aleksandra; Nikolic,Aleksandra; Divac,Aleksandra; Grujic,Milan; Mitic-Milikic,Marija; Radojkovic,Dragica.
The alpha-1-antitrypsin (A1AT) gene is highly polymorphic, with more than 100 genetic variants identified of which some can affect A1AT protein concentration and/or function and lead to pulmonary and/or liver disease. This study reports on the characterization of a p.G320R variant found in two patients, one with emphysema and the other with lung cancer. This variant results from a single base-pair substitution in exon 4 of the A1AT gene, and has been characterized as P by isoelectric focusing. Functional evaluation of the A1AT p.G320R variant was through comparing specific trypsin inhibitory activity in two patients with pulmonary disorders, carriers of the p.G320R variant, and 19 healthy individuals, carriers of normal A1AT M variants. Results showed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-1-antitrypsin; Emphysema; Lung cancer; P variant; Specific trypsin inhibitory activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100002

Development of pollen grain in yellow passion-fruit (Passiflora edulis f. flavicarpa; Passifloraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Margarete Magalhães de; Pereira,Telma Nair Santana.
To clarify events occurring during pollen grain formation in yellow passion-fruit (Passiflora edulis f. flavicarpa), floral buds were collected at different stages of development. After bracket, petal and sepal removal the anthers were fixed, dehydrated, embedded in paraffin wax, sectioned at 10 mum and after differential dying with safranin O and fast green, mounted in Canada balsam and observed under optical microscope. Formation of the male gamete followed the normal pattern for angiosperms. Observation covered final sporogenic mass phase up to pollen grain formation; microsporangium tissue modifications were also observed. Microsporogenesis was characterized by sporogenic tissue differentiation in microsporic mother cells, followed by meiosis and...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200037

Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPD
Provedor de dados: Genetics and Molecular Biology
Autores: Bastianel,Marinês; Schwarz,Sérgio F.; Coleta Filho,Helvécio Della; Lin,Linda Lee; Machado,Marcos; Koller,Otto C.
The randomly amplified polymorphic DNA (RAPD) technique was used to distinguish nucellar and zygotic seedlings resulting from crosses between the ‘Montenegrina’ (Citrus deliciosa Tenore) and‘ King’ (C. nobilis Loureiro) tangerines. The aim of the present study was to develop tangerine varieties with a reduced number of seeds and organoleptic characteristics similar to the ‘Montenegrina’ tangerine. Embryos were isolated from seeds, cultivated in vitro, and acclimated in a greenhouse. Four random primers were used to identify 54 plants of sexual origin from a total of 202 individuals. The degree of polymorphism of each primer was reflected in the number of zygotic plants obtained per primer. Cluster analysis of parents and progeny separated the individuals...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100020

Mutation and association analysis of the PVR and PVRL2 genes in patients with non-syndromic cleft lip and palate
Provedor de dados: Genetics and Molecular Biology
Autores: Sözen,Mehmet A.; Hecht,Jacqueline T.; Spritz,Richard A.
Orofacial clefts (OFC; MIM 119530) are among the most common major birth defects. Here, we carried out mutation screening of the PVR and PVRL2 genes, which are both located at an OFC linkage region at 19q13 (OFC3) and are closely related to PVRL1, which has been associated with both syndromic and non-syndromic cleft lip and palate (nsCLP). We screened a total of 73 nsCLP patients and 105 non-cleft controls from the USA for variants in PVR and PVRL2, including all exons and encompassing all isoforms. We identified four variants in PVR and five in PVRL2. One non-synonymous PVR variant, A67T, was more frequent among nsCLP patients than among normal controls, but this difference did not achieve statistical significance.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: PVR; PVRL2; Cleft lip and palate; Mutation; SSCP.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300007

Cytogenetic and morphometric differences in populations of Astyanax "scabripinnis" (Pisces, Characidae) from Maringá region, PR, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Mizoguchi,Sônia Maria Hiromi Nakagawa; Martins-Santos,Isabel Cristina.
A cytogenetic and morphometric study of four populations of characid fish assigned to the "complex" of Astyanax scabripinnis originating from three riverine basins, namely Ivaí (populations A and B), Paranapanema (C) and Paraná (D), was carried out. Karyological analysis showed the diploid chromosome number 2n = 48 for population B and 2n = 50 for the remaining populations. All populations under study differed in the composition of karyotypes and C-banding patterns. Canonical variable analysis used to assess the morphometric data revealed that 1) the four populations under study were entirely discriminated from each other and 2) the coefficients that most contributed to this pattern were standard length, rostrodorsal distance, eye diameter and snout...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100010

Molecular variability in the maize grey leaf spot pathogens in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Brunelli,Kátia R.; Dunkle,Larry D.; Sobrinho,Cândido A.; Fazza,Ana C.; Camargo,Luis E. A.
Isolates of  Cercospora  species from leaves displaying symptoms of grey leaf spot were collected in maize-producing areas of south-central Brazil in 2001 and 2002. Restriction digests of the internal transcribed spacer region of rDNA detected the presence of the same two  Cercospora  species described on maize in the United States, namely  C. zeae-maydis  and the recently described species,  C. zeina . Genetic variability among isolates was assessed by analysing 104 amplified fragment length polymorphism loci. Cluster analysis confirmed the genetic separation of isolates into two species with a mean similarity of 35%. Similarity levels within species were high, averaging 93% and 92% among isolates of  C. zeae-maydis  and  C. zeina , respectively. The mean...
Palavras-chave: Cercospora zeae-maydis; Cercospora zeina; Epidemiology.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500022

Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Fabiana Aparecida; Marcolino-Gomes,Juliana; Carvalho,Josirlei de Fátima Corrêa; Nascimento,Leandro Costa do; Neumaier,Norman; Farias,José Renato Bouças; Carazzolle,Marcelo Falsarella; Marcelino,Francismar Corrêa; Nepomuceno,Alexandre Lima.
Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; Drought; Transcriptome.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200011

CYP1A1 and CYP2E1 polymorphism frequencies in a large Brazilian population
Provedor de dados: Genetics and Molecular Biology
Autores: Coura,Renata dos Santos; Marques,Christiane de Fátima Silva; Koifman,Rosalina J.; Koifman,Sergio; Cabello,Pedro Hernan; Hatagima,Ana.
The enzymes encoded by the polymorphic genes CYP1A1 and CYP2E1 play an important role in the activation and inactivation of xenobiotics. These enzymes have been associated with xenobiotic-induced diseases, such as cancer, therapeutic failure and adverse effects of drugs. The aim of the present study was to determine the allelic and genotypic frequencies of these polymorphisms in a large, ethnically mixed Brazilian population sample from Rio de Janeiro. Polymorphisms CYP1A1 and CYP2E1 were determined in 870 unrelated individuals by PCR-RFLP analysis in peripheral blood DNA. The observed allelic frequencies were 0.90 for CYP1A1*1A and 0.95 for CYP2E1*1A, in the total sample. The allelic frequency of CYP1A1*2C in "pardos" (0.13) and Brazilian whites (0.11)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytochromes P450; CYP1A1; CYP2E1; Brazil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100001

Phylogenetic position of Placozoa based on large subunit (LSU) and small subunit (SSU) rRNA genes
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Fernanda Britto da; Muschner,Valéria C.; Bonatto,Sandro L.
The phylum Placozoa, represented by its single species Trichoplax adhaerens, has always been considered one of the most basal animal clades due to its low morphological complexity. However, despite its importance, the phylogenetic position of the Placozoa remains uncertain, as does the relationships between the basal metazoans. We sequenced the nearly complete large subunit (LSU) of the T. adhaerens rRNA gene together with the small subunit (SSU) rRNA and used several methods to infer the phylogenetic position of Placozoa and the relationships between the basal animal phyla. The phylogenetic trees obtained from the LSU, SSU and SSU + LSU datasets support monophyly of all basal metazoan phyla, except the Porifera. However, the relationships between all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichoplax adhaerens; Placozoa; Animal basal phylogeny; Metazoa relationship; RRNA genes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100022

Molecular cloning and expression of key gene encoding hypothetical DNA polymerase from B. mori parvo-like virus
Provedor de dados: Genetics and Molecular Biology
Autores: Zhang,Junhong; Li,Guohui; Chen,Huiqing; Li,Xiaogang; Lv,Meng; Chen,Keping; Yao,Qin.
BmPLV-Z is the abbreviation for Bombyx mori parvo-like virus (China isolate). This is a novel virus with two single-stranded linear DNA molecules, viz., VD1 (6543 bp) and VD2 (6022 bp), which are encapsidated respectively into separate virions. Analysis of the deduced amino acid sequence of VD1-ORF4 indicated the existence of a putative DNA-polymerase with exonuclease activity, possibly involved in the replication of BmPLV-Z. In the present study, a recombinant baculovirus was constructed to express the full length of the protein encoded by the VD1-ORF4 gene (3318 bp). In addition, a 2163-bp fragment amplified from the very same gene was cloned into prokaryotic expression vector pET-30a and expressed in E.coli Rosetta 2 (DE3) pLysS. The expressed fusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bombyx mori parvo-like virus; DNA polymerase; Recombinant protein; Sf-9.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400021

New resistance genes in the Zea mays: exserohilum turcicum pathosystem
Provedor de dados: Genetics and Molecular Biology
Autores: Ogliari,Juliana Bernardi; Guimarães,Marco Antônio; Geraldi,Isaías Olívio; Camargo,Luis Eduardo Aranha.
The use of monogenic race-specific resistance is widespread for the control of maize (Zea mays L.) helminthosporiosis caused by Exserohilum turcicum. Inoculation of 18 Brazilian isolates of E. turcicum onto elite maize lines containing previously identified resistance genes and onto differential near-isogenic lines allowed the identification of new qualitative resistance genes. The inoculation of one selected isolate on differential near-isogenic lines, F1 generations and a BC1F1 population from the referred elite lines enabled the characterization of the resistance spectrum of three new genes, one dominant (HtP), one recessive (rt) and a third with non-identified genetic action. Three physiological races of the pathogen were also identified including two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Northern leaf blight; Genetic inheritance; Resistance genes; Races; Maize.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300017

Protective effect of butylated hydroxytoluene (BHT) against the clastogenic acitivity of cadmium chloride and potassium dichromate in hamster ovary cells
Provedor de dados: Genetics and Molecular Biology
Autores: Grillo,Claudia A.; Seoane,Analía I.; Dulout,Fernando N.
The effect of butylated hydroxytoluene (BHT), a widely used food additive, on chromosomal alterations induced by cadmium chloride (CC) and potassium dichromate (PD) in Chinese hamster ovary (CHO) cells was studied both at metaphase and anaphase-telophase. CHO cells were cultured for 15-16 h in the presence of PD (6.0, 9.0 or 12.0 &lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;M), BHT (1.0 &lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;g/ml), or PD plus BHT as well as CC (0.5, 1.0 and 2.0 &lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;M), BHT or CC plus BHT for the analysis of chromosomal aberrations. To perform the anaphase-telophase test, cells were cultured in cover glasses and treated 8 h before fixation with the same chemicals. An extra dose of CC (4 &lt;FONT...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100012

Linkage and mapping of quantitative trait loci associated with angular leaf spot and powdery mildew resistance in common beans
Provedor de dados: Genetics and Molecular Biology
Autores: Bassi,Denis; Briñez,Boris; Rosa,Juliana Santa; Oblessuc,Paula Rodrigues; Almeida,Caléo Panhoca de; Nucci,Stella Maris; Silva,Larissa Chariel Domingos da; Chiorato,Alisson Fernando; Vianello,Rosana Pereira; Camargo,Luis Eduardo Aranha; Blair,Matthew Wohlgemuth; Benchimol-Reis,Luciana Lasry.
Abstract Angular leaf spot (ALS) and powdery mildew (PWM) are two important fungi diseases causing significant yield losses in common beans. In this study, a new genetic linkage map was constructed using single sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs), in a segregating population derived from the AND 277 x SEA 5 cross, with 105 recombinant inbred lines. Phenotypic evaluations were performed in the greenhouse to identify quantitative trait loci (QTLs) associated with resistance by means of the composite interval mapping analysis. Four QTLs were identified for ALS resistance. The QTL ALS11AS, linked on the SNP BAR 5054, mapped on chromosome Pv11, showed the greatest effect (R2 = 26.5%) on ALS phenotypic variance. For PWM resistance,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudocercospora griseola; Erysiphe polygoni; Quantitative inheritance; SSRs; SNPs.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100109

Genetic variation in cultivated Rheum tanguticum populations
Provedor de dados: Genetics and Molecular Biology
Autores: Hu,Yanping; Xie,Xiaolong; Wang,Li; Zhang,Huaigang; Yang,Jian; Li,Yi.
To examine whether cultivation reduced genetic variation in the important Chinese medicinal plant Rheum tanguticum, the levels and distribution of genetic variation were investigated using ISSR markers. Fifty-eight R. tanguticum individuals from five cultivated populations were studied. Thirteen primers were used and a total of 320 DNA bands were scored. High levels of genetic diversity were detected in cultivated R. tanguticum (PPB = 82.19, H = 0.2498, H B = 0.3231, I = 0.3812) and could be explained by the outcrossing system, as well as long-lived and human-mediated seed exchanges. Analysis of molecular variance (AMOVA) showed that more genetic variation was found within populations (76.1%) than among them (23.9%). This was supported by the coefficient...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cultivated populations; Genetic variation; ISSR; Polygonaceae; Rheum tanguticum Maxim. ex Balf.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400010

Testing the Rio Doce as a riverine barrier in shaping the Atlantic rainforest population divergence in the rodent Akodon cursor
Provedor de dados: Genetics and Molecular Biology
Autores: Colombi,Victor Hugo; Lopes,Silvia Ramira; Fagundes,Valéria.
Akodon cursor occurs in dense rainforest from northern (8º S) to southern (26º S) states along the Atlantic coast of Brazil. Previous karyological and molecular data revealed two major clades, one including northern (8-15º S) and the other southern (19-26º S) populations. The center of geographic distribution (15-20º S), which included the state of Espírito Santo, was identified as a potential vicariance region. Since river barriers are among the most discussed models in the study of Neotropical diversification, we examined whether the Rio Doce (19º S) plays an important role in shaping the population genetic divergence of A. cursor by including samples from Espírito Santo in the analysis. Our results showed that the northern-southern division region in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Akodontini; Atlantic Forest; Cytochrome b; Gene flow; Phylogeography; Rodentia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400029

Estimates of genetic parameters of wood traits for sawn timber production in Eucalyptus grandis
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Paulo Eduardo Telles dos; Geraldi,Isaias Olívio; Garcia,José Nivaldo.
In this study, the breeding perspectives of 41 open-pollinated progenies of Eucalyptus grandis were evaluated based on their wood traits. The progenies were distributed in two experiments in a randomized complete block design, with three replicates and linear plots containing six plants each. The traits were assessed at eight years of age. Two trees from each plot were selected for this assessment based on better growth, stem form and phytosanity. Significant differences in basic density, sapwood/heartwood ratio, bowing, specific gravity, parallel compression and static bending were detected among the progenies. These traits were potentially promising for breeding programs, with heritability coefficients that varied from 0.34 to 0.61 on a progeny mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breeding; Eucalypt; Genetic parameters; Wood quality.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400017

RAPD-PCR typing of  Yersinia enterocolitica (Enterobacteriaceae) O:3 serotype strains isolated from pigs and humans
Provedor de dados: Genetics and Molecular Biology
Autores: Leal,Tereza Cristina A.; Leal,Nilma C.; Almeida,Alzira M. Paiva de.
Sixteen strains of Yersinia enterocolitica serotype O:3, isolated from apparently healthy pigs collected in Rio de Janeiro, and four human strains of serotypes O:4, O:5, O:6 and O:13 were analyzed by RAPD-PCR. The strains were grouped into five genotypic profiles according to the amplification patterns obtained with three random primers. Fifteen of the 16 pig strains had identical amplification patterns, which was named genotypic profile 1. The one different profile was named genotypic profile 2. Genotypic profile 1 was also exhibited by the O:6 human serotype strain. The O:4 and O:13 human serotype strains showed similar amplification profiles with two primers. However, the third primer induced a distinct profile in each strain. Therefore, these two...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300005

Cytogenetic and morphological diversity in populations of Astyanax fasciatus (Teleostei, Characidae) from Brazilian northeastern river basins
Provedor de dados: Genetics and Molecular Biology
Autores: Medrado,Aline Souza; Figueiredo,Alba Vivian Amaral; Waldschmidt,Ana Maria; Affonso,Paulo Roberto Antunes de Mello; Carneiro,Paulo Luiz Souza.
In the present work, morphometric and cytogenetic analyses were carried out in populations of the fish Astyanax fasciatus (Characidae) from Contas and Recôncavo Sul River basins (State of Bahia, Brazil), providing new data on the genetic structure of this species along the region. Based on morphologic measurements, we observed that populations from the same hydrographic basin were more similar to each other (Contas and Preto do Costa Rivers), and remarkably divergent from Recôncavo Sul (Mineiro Stream), as indicated by clustering analysis. Cytogenetic data revealed a same diploid number for all populations (2n = 48), but distinct karyotype formulae (8M+24SM+12ST+4A, FN = 92 in Contas River, 8M+24SM+10ST+6A, FN = 90 in Preto do Costa River, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canonic variables; Karyotype; Morphometry; NOR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200007

Genetic diversity of Bemisia tabaci (Genn.) Populations in Brazil revealed by RAPD markers
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,L.H.C.; Campos,L.; Moretzsohn,M.C.; Návia,D.; Oliveira,M.R.V. de.
Bemisia tabaci (Genn.) was considered a secondary pest in Brazil until 1990, despite being an efficient geminivirus vector in beans and soybean. In 1991, a new biotype, known as B. tabaci B biotype (=B. argentifolii) was detected attacking weed plants and causing phytotoxic problems in Cucurbitaceae. Nowadays, B. tabaci is considered one of the most damaging whitefly pests in agricultural systems worldwide that transmits more than 60 different plant viruses. Little is known about the genetic variability of these populations in Brazil. Knowledge of the genetic variation within whitefly populations is necessary for their efficient control and management. The objectives of the present study were to use RAPD markers (1) to estimate the genetic diversity of B....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Whitefly; Molecular markers; UPGMA; AMOVA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200016

DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
Provedor de dados: Genetics and Molecular Biology
Autores: Maia,Thais Augusta; Vilaça,Sibelle Torres; Silva,Luciana Resende da; Santos,Fabricio Rodrigues; Dantas,Gisele Pires de Mendonça.
Abstract This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a ‘least concern’ species. Genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Noninvasive samples; Genetic diversity; Endangered birds; Mergus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808

Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
Provedor de dados: Genetics and Molecular Biology
Autores: Christofoletti,Cintya A.; David,José Augusto O.; Fontanetti,Carmem S.
The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH &gt; 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Comet assay; Ethidium bromide; Silver staining; Tilapia.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100024

Distinct nonrandom patterns of chromosomal deletions in giant-cell lesions of bone
Provedor de dados: Genetics and Molecular Biology
Autores: Baruffi,Marcelo Razera; Barbieri-Neto,J.; Pina-Neto,João Monteiro; Suerzut,C.E.; Casartelli,Cacilda.
Cytogenetic analyses were performed on a bone giant cell reparative granuloma (GCRG) and on three bone giant cell tumors (GCT). The present GCRG case is the second to be described cytogenetically. A modal chromosome number of 46 was observed in all samples. Clonal chromosome abnormalities were detected in all cases. The numerical alterations most frequently observed involved the loss of chromosomes 17 and 18. Among the structural anomalies observed, there was preferential involvement of chromosomes 6 and 10. Three GCT cases presented del(10)(p13) and two cases presented del(6)(q25) (1 GCRG and 1 GCT). These breakpoints mapped on 10p and 6q may harbour genes of importance in the development of bone giant cell tumors.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone; Giant-cell; Granuloma; Tumor; Chromosome.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300002

Proportional odds model applied to mapping of disease resistance genes in plants
Provedor de dados: Genetics and Molecular Biology
Autores: Spyrides-Cunha,Maria Helena; Demétrio,Clarice G.B.; Camargo,Luis E.A.
Molecular markers have been used extensively to map quantitative trait loci (QTL) controlling disease resistance in plants. Mapping is usually done by establishing a statistical association between molecular marker genotypes and quantitative variations in disease resistance. However, most statistical approaches require a continuous distribution of the response variable, a requirement not always met since evaluation of disease resistance is often done using visual ratings based on an ordinal scale of disease severity. This paper discusses the application of the proportional odds model to the mapping of disease resistance genes in plants amenable to expression as ordinal data. The model was used to map two resistance QTL of maize to Puccinia sorghi. The...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100039

Changes in chromosomal polymorphism and global warming: the case of Drosophila subobscura from Apatin (Serbia)
Provedor de dados: Genetics and Molecular Biology
Autores: Zivanovic,Goran; Mestres,Francesc.
In this study, chromosomal inversion polymorphism data for a natural population of Drosophila subobscura from a swampy region near the town of Apatin (Serbia) were compared with data for the same population collected approximately 15 years earlier. The pattern of chromosomal inversion polymorphism changed over time. There were significant increases in the frequency of characteristic southern latitude ("warm" adapted) chromosomal arrangements and significant decreases in the frequency of characteristic northern latitude ("cold" adapted) chromosomal arrangements in the O and U chromosomes. The chromosomal arrangements O3+4 and O3+4+22 (derived from the O3+4 arrangement) showed significant increases in 2008 and 2009 with regard to the 1994 sample. There was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal inversions; Drosophila subobscura; Global warming; Karyotypes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300020

A web-based bioinformatics interface applied to the GENOSOJA project: databases and pipelines
Provedor de dados: Genetics and Molecular Biology
Autores: Nascimento,Leandro Costa do; Costa,Gustavo Gilson Lacerda; Binneck,Eliseu; Pereira,Gonçalo Amarante Guimarães; Carazzolle,Marcelo Falsarella.
The Genosoja consortium is an initiative to integrate different omics research approaches carried out in Brazil. Basically, the aim of the project is to improve the plant by identifying genes involved in responses against stresses that affect domestic production, like drought stress and Asian Rust fungal disease. To do so, the project generated several types of sequence data using different methodologies, most of them sequenced by next generation sequencers. The initial stage of the project is highly dependent on bioinformatics analysis, providing suitable tools and integrated databases. In this work, we describe the main features of the Genosoja web database, including the pipelines to analyze some kinds of data (ESTs, SuperSAGE, microRNAs, subtractive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics; Database; Gene expression; Soybean; Genosoja.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200002

Expression of mdr isoforms in mice during estrous cycle and under hormone stimulation
Provedor de dados: Genetics and Molecular Biology
Autores: Schiengold,Marion; Schwantes,Lavínia; Ribeiro,Maria F.; Lothhammer,Nívia; Gonzalez,Tatiana P.; Chies,Jose Artur Bogo; Nardi,Nance B.
The multidrug resistance (MDR) phenotype is associated with the expression of P-glycoprotein (Pgp), coded by the multigenic mdr family. Mice present the isoforms mdr1 and mdr3, which are responsible for multidrug resistance, and mdr2, that is involved in the transport of phospholipids. mdr1 expression has more recently been associated also with the secretion of steroid hormones. This work presents an RT-PCR analysis of the expression of mdr isoforms, in several organs of mice during different phases of the estrous cycle. Additionally, females were ovariectomized, submitted to different hormone treatments, and their uterus was analyzed for the expression of mdr isoforms. The results show that in the adrenal gland and ovaries mdr1 is the main isoform during...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenal gland; Multidrug resistance; Ovaries; Steroids; Uterus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400029

Analysis of apolipoprotein E genetic polymorphism in a large ethnic Hakka population in southern China
Provedor de dados: Genetics and Molecular Biology
Autores: Zhong,Zhixiong; Wu,Heming; Wu,Hesen; Zhao,Pingsen.
Abstract There is currently no data about the genetic variations of APOE in Hakka population in China. The aim of this study was to analyze the allelic and genotypic frequencies of APOE gene polymorphisms in a large ethnic Hakka population in southern China. The APOE genes of 6,907 subjects were genotyped by the gene chip platform. The allele and genotype frequencies were analyzed. Results showed that the ∊3 allele had the greatest frequency (0.804) followed by ∊2 (0.102), and ∊4 (0.094), while genotype ∊3/∊3 accounted for 65.43% followed by ∊2/∊3 (15.85%), ∊3/∊4 (14.13%), ∊2/∊4 (3.01%), ∊4/∊4 (0.84%), and ∊2/∊2 (0.74%) in all subjects. The frequencies of the ∊4 allele in Chinese populations were lower than Mongolian and Javanese, while the frequencies of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein E; Genetic polymorphism; Hakka; Southern China; Genotyping.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500742

Description of novel microsatellite loci in the Neotropical fish Prochilodus argenteus and cross-amplification in P. costatus and P. lineatus
Provedor de dados: Genetics and Molecular Biology
Autores: Barbosa,Anna C.D.R.; Galzerani,Felipe; Corrêa,Thaís C.; Galetti Jr.,Pedro M.; Hatanaka,Terumi.
Prochilodus is one of the most important fish resources of South America, in addition to the important role it plays in nutrient cycling of Neotropical rivers. In the present study, we describe the isolation and characterization of nine novel microsatellite loci in Prochilodus argenteus. The number of alleles per polymorphic locus varied from 5 (Par76) to 21 (Par85), revealing a total of 116 alleles. The values of observed and expected heterozygosities ranged from 0.629 (Par69) to 0.926 (Par85 and Par86) and from 0.643 (Par66) to 0.931 (Par80), respectively. Furthermore, the ability of these and other previously described microsatellite markers to amplify orthologous loci was tested in two related species, Prochilodus costatus and Prochilodus lineatus....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cross-species amplification; Enrichment libraries; Microsatellite; Prochilodus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200032

Cytogenetical studies in five Atlantic Anguilliformes fishes
Provedor de dados: Genetics and Molecular Biology
Autores: Vasconcelos,Antonio Jales Moraes; Molina,Wagner Franco.
The order Anguilliformes comprises 15 families, 141 genera and 791 fish species. Eight families had at least one karyotyped species, with a prevalence of 2n = 38 chromosomes and high fundamental numbers (FN). The only exception to this pattern is the family Muraenidae, in which the eight species analyzed presented 2n = 42 chromosomes. Despite of the large number of Anguilliformes species, karyotypic reports are available for only a few representatives. In the present work, a species of Ophichthidae, Myrichthys ocellatus (2n = 38; 8m+14sm+10st+6a; FN = 70) and four species of Muraenidae, Enchelycore nigricans (2n = 42; 6m+8sm+12st+16a; FN = 68), Gymnothorax miliaris (2n = 42; 14m+18sm+10st; FN = 84), G. vicinus (2n = 42; 8m+6sm+28a; FN = 56) and Muraena...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anguilliformes; Fish cytogenetics; Muraenidae; Ophichthidae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100012

Protective effect of carboxymethyl-glucan (CM-G) against DNA damage in patients with advanced prostate cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Magnani,Marciane; Castro-Gomez,Raul Jorge Hernan; Mori,Mateus Prates; Kuasne,Hellen; Gregório,Emerson Pereira; Libos Jr,Farid; Cólus,Ilce Mara de Syllos.
Carboxymethyl-glucan (CM-G) is a soluble derivative from Saccharomyces cerevisiae (1 → 3)(1 → 6)-β-D-glucan. The protective efficiency of CM-G against DNA damage in cells from patients with advanced prostate cancer (PCa), and undergoing Androgen Deprivation Therapy (ADT), was evaluated. DNA damage scores were obtained by the comet assay, both before and after treatment with CM-G. The reduction in DNA damage, ranging from 18% to 87%, with an average of 59%, was not related to the increased number of leukocytes in peripheral blood. The results demonstrate for the first time the protective effect of CM-G against DNA damage in patients with advanced PCa. Among smokers, three presented the highest reduction in DNA damage after treatment with CM-G. There was no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Β-D-glucan; Carboxymethyl-glucan; Prostate cancer.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100023

Impact of the tissue factor pathway inhibitor gene on apoptosis in human vascular smooth muscle cells
Provedor de dados: Genetics and Molecular Biology
Autores: Dong,Xia; Song,Li-ping; Zhu,Dun-wan; Zhang,Hai-ling; Liu,Lan-xia; Leng,Xi-gang.
Tissue factor pathway inhibitor (TFPI) plays a vitally important role in the blood coagulation pathway. Recent studies indicated that TFPI induces apoptosis in vascular smooth-muscle cells (VSMCs) in animals. The present study investigated whether the TFPI gene could also induce apoptosis in human vascular smooth-muscle cells (hVSMCs). Such cells were isolated from human umbilical arteries and subsequently transfected with pIRES-TFPI plasmid (2 μg/mL). MTT assaying and cell counting were applied to measure cell viability and proliferation, RT-PCR was utilized to analyze TFPI gene expression in the cells. Apoptosis was analyzed by fluorescence activated cell sorting (FACS). Several key proteins involved in apoptosis were examined through Western blotting....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue factor pathway inhibitor; Vascular smooth muscle cells; Apoptosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100005

Fluorescent in situ hybridization (FISH) as a diagnostic tool for Williams-Beuren syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Deise Helena de; Moretti-Ferreira,Danilo; Rugolo,Lígia Maria Suppo de Souza.
Fluorescent in situ hybridization (FISH) with commercial probes covering the elastin gene (ELN) was used to determine the frequency of the 7q11.23 deletion in 18 children clinically diagnosed with Williams-Beuren syndrome (WBS). A de novo deletion was detected in 15 of the children (83%). Diagnostic investigation for WBS started late in childhood (median = 5.8 years). All the children showed facial features typical of the syndrome, mental retardation and developmental delay. Over-friendliness was observed in the majority of cases. Clinodactyly of the 5th finger (n = 13), cardiovascular disease (n = 9), loquacity (n = 9), low birthweight (n = 8), and failure to thrive (n = 9) were observed only in those children with the deletion. Respiratory problems (n =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 7q11.23 deletion; ELN; FISH; Williams-Beuren syndrome.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100005

Effect of growth hormone on fatty acid synthase gene expression in porcine adipose tissue cultures
Provedor de dados: Genetics and Molecular Biology
Autores: José,Andrea A.F.B.V.; Alvares,Lúcia. E.; Delgado,Eduardo F.; Coutinho,Luiz L.; Lanna,Dante P.D.
We describe an efficient in vitro assay to test growth hormone effects on mRNA levels and fatty acid synthase (FAS, EC. 2.3.1.85) activity. Swine adipose tissue explants were long-term cultured in medium containing growth hormone and FAS mRNA levels and enzyme activity were measured. We quantified FAS transcripts by competitive reverse transcriptase PCR (RT-PCR) using total RNA from cultured adipose tissue explants and RT-PCR standard-curves were constructed using a cloned 307 bp segment of native FAS cDNA and a shorter fragment from which a 64 bp (competitor, 243 bp) internal sequence had been deleted. A known amount of competitor was added to each PCR as an internal control and µ-actin transcripts were also measured to correct for differences in total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRNA quantification; Enzyme activity; Swine; Cultures of adipose tissue explants; Competitive RT-PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100032

Evolutionary history of the Tip100 transposon in the genus Ipomoea
Provedor de dados: Genetics and Molecular Biology
Autores: Christoff,Ana-Paula; Loreto,Elgion L.S.; Sepel,Lenira M.N.
Tip100 is an Ac-like transposable element that belongs to the hAT superfamily. First discovered in Ipomoea purpurea (common morning glory), it was classified as an autonomous element capable of movement within the genome. As Tip100 data were already available in databases, the sequences of related elements in ten additional species of Ipomoea and five commercial varieties were isolated and analyzed. Evolutionary analysis based on sequence diversity in nuclear ribosomal Internal Transcribed Spacers (ITS), was also applied to compare the evolution of these elements with that of Tip100 in the Ipomoea genus. Tip100 sequences were found in I. purpurea, I. nil, I. indica and I. alba, all of which showed high levels of similarity. The results of phylogenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HAT; Transposable elements; Ac-Ds; Ipomoea; Genome evolution; ITS.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300013

True polyploid meiosis in the human male
Provedor de dados: Genetics and Molecular Biology
Autores: Pearson,Peter L.; Madan,Kamlesh.
Abstract Polyploidy does not usually occur in germinal cells of mammals and other higher vertebrates. We describe a unique example of mosaic autotetraploidy in the meiosis of a human male. Although the original observations were made in the late 1960s, we did not publish them at that time, because we expected to detect further examples that could be described together. However, this did not occur and we have now decided to make the observations available to demonstrate that polyploidy in mammalian male meiosis can arise at a higher frequency than expected by random polyploidization of individual meiotic cells, by either DNA duplication or cell fusion prior to synapsis. This is the first description of a population of primary spermatocytes exhibiting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyploidy; Meiosis; Human; Male.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300410

Origin of the hemoglobin S gene in a northern Brazilian population: the combined effects of  slave trade and internal migrations
Provedor de dados: Genetics and Molecular Biology
Autores: Pante-de-Sousa,Gabriella; Mousinho-Ribeiro,Rita de Cassia; Santos,Eduardo José Melo dos; Zago,Marco Antonio; Guerreiro,João Farias.
We analyzed DNA polymorphisms in the &lt;FONT FACE="Symbol"&gt;b&lt;/font&gt;-globin gene cluster of 30 sickle cell anemia patients from Belém, the capital city of the State of Pará, in order to investigate the origin of the &lt;FONT FACE="Symbol"&gt;b&lt;/font&gt;S mutation. Sixty-seven percent of the &lt;FONT FACE="Symbol"&gt;b&lt;/font&gt;S chromosomes were Bantu type, 30% were Benin type, and 3% were Senegal type. The origin of the &lt;FONT FACE="Symbol"&gt;b&lt;/font&gt;S mutation in this population, estimated on the basis of &lt;FONT FACE="Symbol"&gt;b&lt;/font&gt;S-linked haplotypes, contradicts the historical records of direct slave trade from Africa to the northern region of Brazil. Historical records indicate a lower percentage of people from...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400001

Molecular assessment of the phylogeny and biogeography of a recently diversified endemic group of South American canids (Mammalia: Carnivora: Canidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Tchaicka,Ligia; Freitas,Thales Renato Ochotorena de; Bager,Alex; Vidal,Stela Luengos; Lucherini,Mauro; Iriarte,Agustín; Novaro,Andres; Geffen,Eli; Garcez,Fabricio Silva; Johnson,Warren E.; Wayne,Robert K.; Eizirik,Eduardo.
Abstract To investigate the evolution and biogeography of an endemic group of South American foxes, we examined mitochondrial DNA control region sequences for 118 individuals belonging to all six extant species of the genus Lycalopex. Phylogenetic and molecular dating analyses supported the inference that this genus has undergone a very recent and rapid radiation, stemming from a common ancestor that lived ca. 1 million years ago. The Brazilian endemic L. vetulus was supported as the most basal species in this genus, whereas the most internal group is comprised by the recently diverged (ca. 350,000 years ago) Andean/Patagonian species L. griseus and L. culpaeus. We discuss the inferred phylogenetic relationships and divergence times in the context of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial DNA control region; Lycalopex; Canidae; Carnivora.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300442

Parentage testing and effect of misidentification on the estimation of breeding value in Gir cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Baron,Erica E.; Martinez,Mário L.; Verneque,Rui S.; Coutinho,Luiz L.
A correct relationship among sires is essential for an efficient breeding program. Microsatellite markers were used in progeny tests, to assess the paternity of seventy-four probable offspring of nine Gir dairy sires. A 36% misidentification rate was observed; however, these errors had minimal effects on the ranking of the nine bulls with regard to their genetic values. The results suggest that paternity tests should be performed in breeding programs, in order to prevent inappropriate paternities from influencing the genetic value of bulls in the future.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite; Gir; Cattle; Breeding; Parentage; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400006

Cell wall, lignin and fatty acid-related transcriptome in soybean: achieving gene expression patterns for bioenergy legume
Provedor de dados: Genetics and Molecular Biology
Autores: Pestana-Calsa,Maria Clara; Pacheco,Cinthya Mirella; Castro,Renata Cruz de; Almeida,Renata Rodrigues de; Lira,Nayara Patrícia Vieira de; Calsa Junior,Tercilio.
Increasing efforts to preserve environmental resources have included the development of more efficient technologies to produce energy from renewable sources such as plant biomass, notably through biofuels and cellulosic residues. The relevance of the soybean industry is due mostly to oil and protein production which, although interdependent, results from coordinated gene expression in primary metabolism. Concerning biomass and biodiesel, a comprehensive analysis of gene regulation associated with cell wall components (as polysaccharides and lignin) and fatty acid metabolism may be very useful for finding new strategies in soybean breeding for the expanding bioenergy industry. Searching the Genosoja transcriptional database for enzymes and proteins directly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Genosoja platform; Tissue-specific expression.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200013

New occurrence of B chromosomes in Partamona helleri (Friese, 1900) (Hymenoptera, Meliponini)
Provedor de dados: Genetics and Molecular Biology
Autores: Martins,Cinthia Caroline Cardoso; Duarte,Olivia Maria Pereira; Waldschmidt,Ana Maria; Alves,Rogério Marco de Oliveira; Costa,Marco Antônio.
Cytogenetic analyses of the stingless bee Partamona helleri collected in the state of Bahia, Northeast Brazil revealed the chromosome numbers n = 18 in the haploid males and 2n = 35 in the diploid females. All karyotypes displayed one large acrocentric B chromosome, which differs from the minute B chromosomes previously described in the populations from southeastern Brazil. Giemsa staining, C-banding and DAPI/CMA3 fluorochrome staining also revealed a remarkable interpopulational divergence regarding both the regular karyotype and the B chromosomes. The B chromosomes found in the samples from Jequié, Bahia, were entirely heterochromatic, while those found in Cravolândia, Bahia, displayed a euchromatic portion at the telomeric end of the long arm. CMA3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stingless Bee; Partamona helleri; Geographic variation; Supernumerary chromosomes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400019

Sugarcane phytocystatins: Identification, classification and expression pattern analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Reis,Emerson Moreira; Margis,Rogério.
The cystatins are tightly binding, but reversible, inhibitors of cysteine proteinases, which constitute a superfamily of evolutionary related proteins. They have been subdivided into three families: the cystatin family which contain two disulfide bonds, the stefin family which lack disulfide bonds, and the kininogen family composed of large glycoproteins containing three repeats similar to those found in the cystatin family. Members of the cystatin superfamily occurring in plants are currently known as phytocystatins, defined as proteins lacking disulfide bonds but possessing a conserved N-terminal amino acid sequence (L-A-R-[FY]-A-[VI]-X(3)-N). We have used the protein sequence deduced from seven phytocystatins (from the Arabidopsis thaliana genome...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100038

Erratum
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100151

Constitutional and somatic methylation status of DMRH19 and KvDMR in Wilms tumor patients
Provedor de dados: Genetics and Molecular Biology
Autores: Cardoso,Leila C.A.; Castaño,Jair A. Tenorio; Pereira,Hanna S.; Lima,Maria Angélica de F.D.; Santos,Anna Cláudia E. dos; Faria,Paulo S. de; Ferman,Sima; Seuánez,Héctor N.; Nevado,Julián B.; Almeida,José Carlos Cabral de; Lapunzina,Pablo; Vargas,Fernando R.
The most frequent epigenetic alterations in Wilms tumor (WT) occur at WT2, assigned to 11p15. WT2 consists of two domains: telomeric domain 1 (DMRH19) that contains the IGF2 gene and an imprinted maternally expressed transcript (H19) and centromeric domain 2 (KvDMR) that contains the genes KCNQ1, KCNQ1OT1 and CDKN1C. In this work, we used pyrosequencing and MS-MLPA to compare the methylation patterns of DMRH19/KvDMR in blood and tumor samples from 40 WT patients. Normal constitutional KvDMR methylation indicated that most of the epigenetic alterations in WT occur at DMRH19. Constitutional DMRH19 hypermethylation (HM DMRH19) was observed in two patients with Beckwith-Wiedemann syndrome. Pyrosequencing and MS-MLPA showed HM DMRH19 in 28/34 tumor samples:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epigenetic; Histopathology; Methylation; MS-MLPA; Pyrosequencing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500002

Genetic relationship in Coffea species and parentage determination of interspecific hybrids using ISSR (Inter- Simple Sequence Repeat) markers
Provedor de dados: Genetics and Molecular Biology
Autores: Ruas,Paulo M.; Ruas,Claudete F.; Rampim,Leandro; Carvalho,Valdemar P.; Ruas,Eduardo A.; Sera,Tumoru.
Inter-simple sequence repeat (ISSR) markers were used to evaluate genetic divergence among eight Coffea species and to identify the parentage of six interspecific hybrids. A total of 14 primers which contained different simple sequence repeats (SSR) were used as single primers or combined in pairs and tested for PCR amplifications. Two hundred and thirty highly reproducible fragments were amplified, which were then used to estimate the genetic similarity and to cluster the Coffea species and hybrids. High levels of interspecific genetic variation were revealed. The dinucleotide motif (GA)9T combined with other di- tri- and tetra-nucleotides produced a greater number of DNA fragments, mostly polymorphics, suggesting a high frequency of the poly GA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea; Coffee breeding; DNA markers; ISSR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300017

Genetic diversity among maize (Zea mays L.) landraces assessed by RAPD markers
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Valdemar P.; Ruas,Claudete F.; Ferreira,Josué M.; Moreira,Rosângela M.P.; Ruas,Paulo M.
The genetic relationships among 81 maize accessions consisting 79 landraces and two improved varieties, maintained by farmers in southern Brazil were investigated using Random Amplified Polymorphic DNA (RAPD). Thirty-two highly informative primers amplified 255 markers of which 184 (72.2%) were polymorphics. Based on the RAPD markers, a dendrogram was constructed using the UPGMA method. The range of genetic similarity was from 0.78 to 0.91. The molecular data grouped the accessions into two main clusters, which were correlated according to kernel colors. Small clusters were seen associated to characteristics, such as kernel morphology. The analysis of the molecular data revealed that maize management adopted by small-scale farmers has contributed to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corn landrace; Genetic variability; Molecular genetic markers; RAPD.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200017

Genetic structure and a selected core set of brazilian soybean cultivars
Provedor de dados: Genetics and Molecular Biology
Autores: Priolli,Regina Helena Geribello; Wysmierski,Philip Traldi; Cunha,Camila Pinto da; Pinheiro,José Baldin; Vello,Natal Antonio.
Soybean is one of the most valuable and profitable oil crop species and a thorough knowledge of the genetic structure of this crop is necessary for developing the best breeding strategies. In this study, a representative collection of soybean cultivars recommended for farming in all Brazilian regions was genotyped using 27 simple sequence repeat (SSR) loci. A total of 130 alleles were detected, with an average allelic number of 4.81 per locus. These alleles determined the core set that best represented this soybean germplasm. The Bayesian analysis revealed the presence of two clusters or subgroups within the whole collection (435 soybean cultivars) and the core set (31 entries). Cultivars of similar origin (ancestral) were clustered into the same groups in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Core collection; Genetic base; Glycine max (L.) Merrill; Bayesian method; SSR markers.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300013

Genetic variation among species, races, forms and inbred lines of lac insects belonging to the genus Kerria (Homoptera, Tachardiidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Ranjan,Sanjeev Kumar; Mallick,Chandana Basu; Saha,Dipnarayan; Vidyarthi,Ambarish S; Ramani,Ranganathan.
The lac insects (Homoptera: Tachardiidae), belonging to the genus Kerria, are commercially exploited for the production of lac. Kerria lacca is the most commonly used species in India. RAPD markers were used for assessing genetic variation in forty-eight lines of Kerria, especially among geographic races, infrasubspecific forms, cultivated lines, inbred lines, etc., of K. lacca. In the 48 lines studied, the 26 RAPD primers generated 173 loci, showing 97.7% polymorphism. By using neighbor-joining, the dendrogram generated from the similarity matrix resolved the lines into basically two clusters and outgroups. The major cluster, comprising 32 lines, included mainly cultivated lines of the rangeeni form, geographic races and inbred lines of K. lacca. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA fingerprinting; Genetic variation; Kerria; Lac insects; RAPD; Tachardiidae.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300023

Chromosome 6q deletion: Report of a new case and review of the literature
Provedor de dados: Genetics and Molecular Biology
Autores: Boy,Raquel; Pimentel,Márcia Mattos Gonçalves; Hemerly,Ana Paula; Silva,Maria do Perpétuo Socorro; Barreiro,Ana Paula; Almeida,José Carlos Cabral de; Llerena,Juan.
The authors report an additional case of partial monosomy of the long arm of chromosome 6 [46,XY,del (6)(q22 &lt;font SIZE="3" face="Symbol"&gt;®&lt;/font&gt; qter)]. Our patient has a large segment beyond 6q25 deleted, then severe psychomotor retardation is expected to occur.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100024

Polymorphism of human haptoglobin and its clinical importance
Provedor de dados: Genetics and Molecular Biology
Autores: Wobeto,Vânia Peretti de Albuquerque; Zaccariotto,Tânia Regina; Sonati,Maria de Fátima.
Haptoglobin (Hp) is a plasma glycoprotein, the main biological function of which is to bind free hemoglobin (Hb) and prevent the loss of iron and subsequent kidney damage following intravascular hemolysis. Haptoglobin is also a positive acute-phase protein with immunomodulatory properties. In humans, the HP locus is polymorphic, with two codominant alleles (HP1 and HP2) that yield three distinct genotypes/phenotypes (Hp1-1, Hp2-1 and Hp2-2). The corresponding proteins have structural and functional differences that may influence the susceptibility and/or outcome in several diseases. This article summarizes the available data on the structure and functions of Hp and the possible effects of Hp polymorphism in a number of important human disorders.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haptoglobin; Hemoglobin; Genetic polymorphisms.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400002

Genes encoding enzymes of the lignin biosynthesis pathway in Eucalyptus
Provedor de dados: Genetics and Molecular Biology
Autores: Harakava,Ricardo.
Eucalyptus ESTs libraries were screened for genes involved in lignin biosynthesis. This search was performed under the perspective of recent revisions on the monolignols biosynthetic pathway. Eucalyptus orthologues of all genes of the phenylpropanoid pathway leading to lignin biosynthesis reported in other plant species were identified. A library made with mRNAs extracted from wood was enriched for genes involved in lignin biosynthesis and allowed to infer the isoforms of each gene family that play a major role in wood lignin formation. Analysis of the wood library suggests that, besides the enzymes of the phenylpropanoids pathway, chitinases, laccases, and dirigent proteins are also important for lignification. Colocalization of several enzymes on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus; Lignin; Expressed sequenced tag; Phenylpropanoids pathway; Monolignols.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400015

Genotoxic and antigenotoxic effects of Fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and Comet assays
Provedor de dados: Genetics and Molecular Biology
Autores: Leite-Silva,Cleide; Gusmão,Cássia Lima Silva; Takahashi,Catarina Satie.
The brown seaweed Fucus vesiculosus (Fucales, Fucaceae) was screened for its protective activity using doxorubicin-induced DNA damage in human lymphocytes. In this study, we assessed the genotoxic and antigenotoxic potential of three different concentrations (0.25, 0.5 and 1.0 mg mL-1) of F. vesiculosus aqueous extract using the chromosome aberration and Comet assays. Treatment of human lymphocyte cultures with 0.25, 0.5 and 1.0 mg mL-1 F. vesiculosus aqueous extract had no effect on the chromosome aberration frequency or on the extent of DNA damage detected by the Comet assay. The antigenotoxic effects of the extract were tested in human lymphocyte cultures treated with 15 µg mL-1 of doxorubicin, either alone or combined with the different concentrations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigenotoxicity; Chromosomal aberrations; Comet assay; Doxorubicin; Fucus vesiculosus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100019

Normal microspore production after cell fusion in Brachiaria jubata (Gramineae)
Provedor de dados: Genetics and Molecular Biology
Autores: Mendes-Bonato,Andréa Beatriz; Risso-Pascotto,Claudicéia; Pagliarini,Maria Suely; Valle,Cacilda Borges do.
Cytogenetic studies were carried out on 22 accessions of Brachiaria jubata from the Embrapa Beef Cattle Brachiaria collection. One accession was diploid (2n = 2x = 18) and the remaining 21 were tetraploid (2n = 4x = 36). Among five tetraploid accessions, a specific and constant pattern of cell fusion involving only two microsporocytes was recorded. Meiosis proceeded normally from prophase I to the end, giving rise to an octad with normal microspores that developed into fertile pollen grains. Regular octad formation was possible because each cellular chromosome set was maintained in its proper domain, spindles were correctly positioned, and cytokinesis planes were formed in the correct places. Such behavior of meiosis in syncytes has never been reported in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachiaria jubata; Cell fusion; Microsporogenesis; Pollen fertility.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400016

Genetic diversity of "Pimenta Longa" genotypes (Piper spp., Piperaceae) of the Embrapa Acre germplasm collection
Provedor de dados: Genetics and Molecular Biology
Autores: Wadt,Lúcia Helena de Oliveira; Ehringhaus,Christiane; Kageyama,Paulo Yoshio.
The commonly known Pimenta longa is a commercially valuable natural resource found wild in Acre, Brazil. Specifically, three Piperaceae species with contested taxonomic status were studied, Piper hispidinervum, Piper aduncum, and Piper hispidum, to assesses the inter- and intra-specific genetic relationship of 49 Piper genotypes kept in the Pimenta longa germplasm collection at Embrapa Acre, using sixty six Random Amplified Polymorphic DNA (RAPD) markers. The DNA polymorphism level detected was high (96.97%), but the marker frequencies for each species showed polymorphism levels of 79.4% for Piper hispidinervum and 5.3% for P. aduncum. The genetic similarity clustering analysis resulted in three distinct groups corresponding to Piper hispidinervum, Piper...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Safrole; P. hispidinervum; P. aduncum; P. hispidum; RAPDs.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100013

Genetic structure of Argentinean hexaploid wheat germplasm
Provedor de dados: Genetics and Molecular Biology
Autores: Vanzetti,Leonardo S.; Yerkovich,Nadia; Chialvo,Eugenia; Lombardo,Lucio; Vaschetto,Luis; Helguera,Marcelo.
The identification of genetically homogeneous groups of individuals is an ancient issue in population genetics and in the case of crops like wheat, it can be valuable information for breeding programs, genetic mapping and germplasm resources. In this work we determined the genetic structure of a set of 102 Argentinean bread wheat (Triticum aestivum L.) elite cultivars using 38 biochemical and molecular markers (functional, closely linked to genes and neutral ones) distributed throughout 18 wheat chromosomes. Genetic relationships among these lines were examined using model-based clustering methods. In the analysis three subpopulations were identified which correspond largely to the origin of the germplasm used by the main breeding programs in Argentina.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; Genetic structure; Molecular markers; Genetic diversity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300014

Comparative analysis of the complete chloroplast genomes from six Neotropical species of Myrteae (Myrtaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Nureyev F.; Balbinott,Natalia; Paim,Igor; Guzman,Frank; Margis,Rogerio.
Abstract Myrteae is the largest and most diverse tribe within Myrtaceae and represents the majority of its diversity in the Neotropics. Members of Myrteae hold ecological importance in tropical biomes for the provision of food sources for many animal species. Thus, due to its several roles, a growing interest has been addressed to this group. In this study, we report the sequencing and de novo assembly of the complete chloroplast (cp) genomes of six Myrteae species: Eugenia brasiliensis, E. pyriformis, E. nitida, Myrcianthes pungens, Plinia edulis and Psidium cattleianum. We characterized genome structure, gene content, and identified SSRs to detect variation within Neotropical Myrteae. The six newly sequenced plastomes exhibit a typical quadripartite...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CpDNA; Genomic resource; Populational genetics; Plastid; Conservation.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400801

Using linear algebra for protein structural comparison and classification
Provedor de dados: Genetics and Molecular Biology
Autores: Gomide,Janaína; Melo-Minardi,Raquel; Santos,Marcos Augusto dos; Neshich,Goran; Meira Jr.,Wagner; Lopes,Júlio César; Santoro,Marcelo.
In this article, we describe a novel methodology to extract semantic characteristics from protein structures using linear algebra in order to compose structural signature vectors which may be used efficiently to compare and classify protein structures into fold families. These signatures are built from the pattern of hydrophobic intrachain interactions using Singular Value Decomposition (SVD) and Latent Semantic Indexing (LSI) techniques. Considering proteins as documents and contacts as terms, we have built a retrieval system which is able to find conserved contacts in samples of myoglobin fold family and to retrieve these proteins among proteins of varied folds with precision of up to 80%. The classifier is a web tool available at our laboratory website....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein classification; Contact maps; Linear algebra; Singular value decomposition; Latent semantic indexing.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300032

Simultaneous quantitative assessment of circulating cell-free mitochondrial and nuclear DNA by multiplex real-time PCR
Provedor de dados: Genetics and Molecular Biology
Autores: Xia,Peng; Radpour,Ramin; Zachariah,Rebecca; Fan,Alex Xiu Cheng; Kohler,Corina; Hahn,Sinuhe; Holzgreve,Wolfgang; Zhong,Xiao Yan.
Quantification of circulating nucleic acids in plasma and serum could be used as a non-invasive diagnostic tool for monitoring a wide variety of diseases and conditions. We describe here a rapid, simple and accurate multiplex real-time PCR method for direct synchronized analysis of circulating cell-free (ccf) mitochondrial (mtDNA) and nuclear (nDNA) DNA in plasma and serum samples. The method is based on one-step multiplex real-time PCR using a FAM-labeled MGB probe and primers to amplify the mtDNA sequence of the ATP 8 gene, and a VIC-labeled MGB probe and primers to amplify the nDNA sequence of the glycerinaldehyde-3-phosphate-dehydrogenase (GAPDH) gene, in plasma and serum samples simultaneously. The efficiencies of the multiplex assays were measured in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circulating cell-free DNA; Mitochondrial DNA; Nuclear DNA; Real-time PCR; Quantitative PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100003

Chromosome polymorphism in the Brazilian dwarf brocket deer, Mazama nana (Mammalia, Cervidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Abril,Vanessa Veltrini; Duarte,José Maurício Barbanti.
The Brazilian dwarf brocket deer (Mazama nana) is the smallest deer species in Brazil and is considered threatened due to the reduction and alteration of its habitat, the Atlantic Rainforest. Moreover, previous work suggested the presence of intraspecific chromosome polymorphisms which may contribute to further population instability because of the reduced fertility arising from the deleterious effects of chromosome rearrangements during meiosis. We used G- and C-banding, and nucleolus organizer regions localization by silver-nitrate staining (Ag-NOR) to investigate the causes of this variation. Mazama nana exhibited eight different karyotypes (2n = 36 through 39 and FN = 58) resulting from centric fusions and from inter and intraindividual variation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mazama nana; Brazilian dwarf brocket deer; Cytogenetics; Chromosome polymorphism; Centric fusions.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100011

Transcriptome sequencing analysis of alfalfa reveals CBF genes potentially playing important roles in response to freezing stress
Provedor de dados: Genetics and Molecular Biology
Autores: Shu,Yongjun; Li,Wei; Zhao,Jinyue; Zhang,Sijia; Xu,Hanyun; Liu,Ying; Guo,Changhong.
Abstract Alfalfa (Medicago sativa L.) is an important perennial forage, with high nutritional value, which is widely grown in the world. Because of low freezing tolerance, its distribution and production are threatened and limited by winter weather. To understand the complex regulation mechanisms of freezing tolerance in alfalfa, we performed transcriptome sequencing analysis under cold (4 °C) and freezing (-8 °C) stresses. More than 66 million reads were generated, and we identified 5767 transcripts differentially expressed in response to cold and/or freezing stresses. These results showed that these genes were mainly classified as response to stress, transcription regulation, hormone signaling pathway, antioxidant, nodule morphogenesis, etc., implying...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicago sativa L.; RNA-seq; Freezing tolerance; C-repeat binding factors (CBF).
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500824

New biotechnological tools to accelerate scab-resistance trait transfer to apple
Provedor de dados: Genetics and Molecular Biology
Autores: Cusin,Roberta; Revers,Luís Fernando; Maraschin,Felipe dos Santos.
Abstract Apple is a fruit crop cultivated worldwide. Apple orchards are exposed to a diverse set of environmental and biological factors that affect the productivity and sustainability of the culture. Many of the efforts and costs for apple production rely on reducing the incidence of fungal diseases, and one of the main diseases is apple scab caused by the fungus Venturia inaequalis. The economic impact of scab on apple productivity has guided many breeding programs to search for cultivars resistant to apple scab. Introgression from wild relatives has been successful to some extent, and genetic engineering for resistant cultivars has even been employed. This review presents the techniques used to the present time to obtain pathogen-resistant apple...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malus; Venturia inaequalis; Biotic stress; Transformation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200305

Meiotic behavior of several Brazilian soybean varieties
Provedor de dados: Genetics and Molecular Biology
Autores: Bione,Nilton Cesar Pires; Pagliarini,Maria Suely; Toledo,José Francisco Ferraz de.
Despite the importance of soybeans little cytogenetic work has traditionally been done, due to the small size and apparent similarity of the chromosomes. Fifteen soybean [Glycine max (L.) Merrill] varieties adapted for cultivation in two distinct regions of Brazil were analyzed cytogenetically. A low frequency of meiotic abnormalities was noted in all varieties, although they were not equally affected. Irregular chromosome segregation, chromosome stickiness, cytoplasmic connections between cells, cytomixis and irregular spindles were the main abnormalities observed, none of which had been described previously in soybeans. All of these abnormalities can affect pollen fertility. Pollen fertility was high in most varieties and was correlated with meiotic...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300022

Mutational analysis of the GAP-related domain of the neurofibromatosis type 1 gene in Brazilian NF1 patients
Provedor de dados: Genetics and Molecular Biology
Autores: Trovó,Alessandra B.; Goloni-Bertollo,Eny M.; Mancini,Ulises M.; Rahal,Paula; Azevedo Jr.,Walter F. de; Tajara,Eloiza H.
Neurofibromatosis type 1 (NF1) is a common autosomal dominant disorder caused by mutations in the NF1 gene. In the present study, a total of 55 unrelated NF1 patients were screened for mutations in the GAP-related domain/GRD (exons 20-27a) by single-strand conformation polymorphism (SSCP). Four different mutations were identified and, taken together, they comprise one nonsense substitution (Q1189X), one deletion (3525-3526delAA), one missense substitution (E1356G) and one mutation in the splice acceptor site (c.4111-1G&gt;A). One novel polymorphism (c.4514+11C&gt;G) and other three putative polymorphisms were also found (c.3315-27G&gt;A, V1146I and V1317A). Genotype-phenotype correlations were investigated, but no particular association was detected.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene NF1; GRD; Neurofibromatosis type 1; Mutations; Polymorphisms.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300003

B chromosomes in a population of Astyanax eigenmanniorum (Characiformes, Characidae) from the Araguari River Basin (Uberlândia, MG, Brazil)
Provedor de dados: Genetics and Molecular Biology
Autores: Torres-Mariano,Alessandra Ribeiro; Morelli,Sandra.
A cytogenetic study was conducted on an Astyanax eigenmanniorum population from the Caetano Stream (18° 44' 56" S/ 048° 18' 39" W) - in Uberlândia, MG, Brazil - showing a modal diploid number of 48 chromosomes in the standard male and female karyotypes. However, in several specimens it was also possible to observe metaphases with one or two B chromosomes, increasing the diploid number to 49 or 50 chromosomes, respectively. The supernumerary chromosomes were totally heterochromatic and highlighted after C-banding. The silver-stained nucleolus organizing regions (Ag-NORs) were located in at least five chromosomes of the standard karyotype, thus characterizing a multiple NOR system in the species. This is the first occurrence of an A. eigenmanniorum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characidae; Astyanax eigenmanniorum; Supernumerary chromosome; Heterochromatin; Nucleolus organizing regions (NORs).
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200015

Chimerism 47,XY,+21/46,XX in a female infant with anencephaly and other congenital defects
Provedor de dados: Genetics and Molecular Biology
Autores: Lucon,Danielle R.; Zanchetta,Luciene M.; Cavalcanti,Denise P.
Chimerism is rare in humans and is usually discovered accidentally when a 46,XX and 46,XY karyotype is found in a same individual. We describe a malformed female infant with neural tube defect (NTD) and a 47,XY,+21[5]/46,XX[30] karyotype.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chimerism; Anencephaly; Trisomy 21.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100006

An illustrative case of Léri-Weill dyschondrosteosis
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Renata de; Iamada,Cristina Forti; Silva,Luciana Oliveira; Mello,Maricilda Palandi de; Maciel-Guerra,Andréa Trevas.
We report on a girl presenting Léri-Weill dyschondrosteosis (LWD) due to deletion of the  SHOX  gene. Her family included individuals with short stature alone or with both short stature and mesomelia or Madelung's deformity. The deletion was demonstrated through detection of hemizygosity for microsatellite markers SHOX-CA repeat, DXYS10092, DXYS10093 and DXYS10091 localized around the  SHOX  gene, with retention of paternal alleles in the proband and three of her sisters who had short stature as the only clinical feature. Hemizygosity for these loci was also observed in their mother, who had short stature too. The deletion in the proband was however larger, including locus DXY10083. The proband's only sister with normal height did not carry the deletion....
Palavras-chave: Léri-Weill dyschondrosteosis; Madelung's deformity; Pseudoautosomal dominant inheritance; Short stature; SHOX  gene.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500007

Enzyme replacement therapy for Mucopolysaccharidosis Type I among patients followed within the MPS Brazil Network
Provedor de dados: Genetics and Molecular Biology
Autores: Dornelles,Alícia Dorneles; Pinto,Louise Lapagesse de Camargo; Paula,Ana Carolina de; Steiner,Carlos Eduardo; Lourenço,Charles Marques; Kim,Chong Ae; Horovitz,Dafne Dain Gandelman; Ribeiro,Erlane Marques; Valadares,Eugênia Ribeiro; Goulart,Isabela; Souza,Isabel C. Neves de; Neri,João Ivanildo da Costa; Santana-da-Silva,Luiz Carlos; Silva,Luiz Roberto; Ribeiro,Márcia; Oliveira Sobrinho,Ruy Pires de; Giuglianiand,Roberto; Schwartz,Ida Vanessa Doederlein.
Mucopolysaccharidosis type I (MPS I) is a rare lysosomal disorder caused by deficiency of alph-L-iduronidase. Few clinical trials have assessed the effect of enzyme replacement therapy (ERT) for this condition. We conducted an exploratory, open-label, non-randomized, multicenter cohort study of patients with MPS I. Data were collected from questionnaires completed by attending physicians at the time of diagnosis (T1; n = 34) and at a median time of 2.5 years later (T2; n = 24/34). The 24 patients for whom data were available at T2 were allocated into groups: A, no ERT (9 patients; median age at T1 = 36 months; 6 with severe phenotype); B, on ERT (15 patients; median age at T1 = 33 months; 4 with severe phenotype). For all variables in which there was no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme replacement therapy; Laronidase; Mucopolysaccharidosis Type I; Alph-L-iduronidase.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100006

The insulin polymorphism -23Hph increases the risk for type 1 diabetes mellitus in the Romanian population
Provedor de dados: Genetics and Molecular Biology
Autores: Cimponeriu,Danut; Apostol,Pompilia; Radu,Irina; Craciun,Anne Marie; Serafinceanu,Cristian; Toma,Mihai; Panaite,Cristian; Cheta,Dan.
The insulin -23Hph and IGF2 Apa polymorphisms were genotyped in Romanian patients with T1DM (n = 204), T2DM (n = 215) or obesity (n = 200) and normoponderal healthy subjects (n = 750). The genotypes of both polymorphisms were distributed in concordance with Hardy-Weinberg equilibrium in all groups. The -23Hph AA genotype increased the risk for T1DM (OR: 3.22, 95%CI: 2.09-4.98, p &lt; 0,0001), especially in patients without macroalbuminuria (OR: 4.32, 95%CI: 2.54-7.45, p &lt; 0,0001). No other significant association between the alleles or genotypes of insulin -23Hph and IGF2 Apa and diabetes or obesity was identified.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin -23Hph; Insulin-like growth factor 2 Apa; Diabetes; Obesity; Case-control study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400003

Frequencies of CCR5-D32, CCR2-64I and SDF1-3’A mutations in Human Immunodeficiency Virus (HIV) seropositive subjects and seronegative individuals from the state of Pará in Brazilian Amazonia
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalhaes,Fernanda Andreza de Pinho Lott; Cardoso,Greice Lemos; Vallinoto,Antonio Carlos Rosário; Machado,Luiz Fernando; Ishak,Marluisa de Oliveira Guimarães; Ishak,Ricardo; Guerreiro,João Farias.
The distribution of genetic polymorphisms of chemokine receptors CCR5-delta32, CCR2-64I and chemokine (SDF1-3’A) mutations were studied in 110 Human Immunodeficiency Virus type 1 (HIV-1) seropositive individuals (seropositive group) and 139 seronegative individuals (seronegative group) from the population of the northern Brazilian city of Belém which is the capital of the state of Pará in the Brazilian Amazon. The CCR5-delta32 mutation was found in the two groups at similar frequencies, i.e. 2.2% for the seronegative group and 2.7% for the seropositive group. The frequencies of the SDF1-3’A mutation were 21.0% for the seronegative group and 15.4% for the seropositive group, and the CCR2-64I allele was found at frequencies of 12.5% for the seronegative...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CCR5; CCR2; SDF-1; HIV-1 infection; Allele frequency; Brazilian Amazon.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500004

Chromosome numbers in Bromeliaceae
Provedor de dados: Genetics and Molecular Biology
Autores: Cotias-de-Oliveira,Ana Lúcia Pires; Assis,José Geraldo Aquino de; Bellintani,Moema Cortizo; Andrade,Jorge Clarêncio Souza; Guedes,Maria Lenise Silva.
The present study reports chromosome numbers of 17 species of Bromeliaceae, belonging to the genera Encholirium, Bromelia, Orthophytum, Hohenbergia, Billbergia, Neoglaziovia, Aechmea, Cryptanthus and Ananas. Most species present 2n = 50, however, Bromelia laciniosa, Orthophytum burle-marxii and O. maracasense are polyploids with 2n = 150, 2n = 100 and 2n = 150, respectively, while for Cryptanthus bahianus, 2n = 34 + 1-4B. B chromosomes were observed in Bromelia plumieri and Hohenbergia aff. utriculosa. The chromosome number of all species was determined for the first time, except for Billbergia chlorosticta and Cryptanthus bahianus. Our data supports the hypothesis of a basic number of x = 25 for the Bromeliaceae family and decreasing aneuploidy in the...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100032

The karyotype of the critically endangered Lear's macaw, Anodorhynchus leari Bonaparte 1856 (Aves, Psittaciformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Nogueira,Denise Monnerat; Souza,Lucia Moreno de; Goldschmidt,Beatriz; Silva,Christiano Pinheiro da; Monsores,Denise Wilches.
We used conventional chromosomal staining to describe the karyotype of the critically endangered Lear's macaw, Anodorhynchus leari Bonaparte 1856. A diploid number of 2n = 70 and a karyotype similar to that of its congener Anodorhynchus hyacinthinus suggests that chromosomal rearrangements were not the main evolutionary mechanism in the genus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birds; Karyotype; Chromosomes; Psittaciformes; Endangered species.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400014

Taxonomic and evolutionary analysis of Zaprionus indianus and its colonization of Palearctic and Neotropical regions
Provedor de dados: Genetics and Molecular Biology
Autores: Commar,Leliane Silva; Galego,Luis Gustavo da Conceição; Ceron,Carlos Roberto; Carareto,Claudia Marcia Aparecida.
Zaprionus indianus is a dipteran (Drosophilidae) with a wide distribution throughout the tropics and temperate Palearctic and Nearctic regions. There have been proposals to reclassify the genus Zaprionus as a subgenus or group of the genus Drosophila because various molecular markers have indicated a close relationship between Zaprionus species and the immigrans-Hirtodrosophila radiation within Drosophila. These markers, together with alloenzymes and quantitative traits, have been used to describe the probable scenario for the expansion of Zaprionus indianus from its center of dispersal (Africa) to regions of Asia (ancient dispersal) and the Americas (recent dispersal). The introduction of Z. indianus into Brazil was first reported in 1999 and the current...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alloenzyme; Bioinvasion; Molecular markers; Phylogenetic analysis; Quantitative traits.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300003

Ribeiro's typology, genomes, and Spanish colonialism, as viewed from Gran Canaria and Colombia
Provedor de dados: Genetics and Molecular Biology
Autores: Bortolini,Maria Cátira; Thomas,Mark G.; Chikhi,Lourdes; Aguilar,Juan A.; Castro-De-Guerra,Dinorah; Salzano,Francisco M.; Ruiz-Linares,Andres.
Four biallelic and six multiallelic Y-chromosome polymorphisms were investigated in 59 Gran Canarian, 60 North African Berber and 46 Spanish subjects. These new data were merged with equivalent literature information to obtain the parental Y-chrosomomal contribution in Gran Canarians, Colombians, and Venezuelans. The results were then compared, for Gran Canarians and Colombians, to those derived from autosomal and mtDNA. In both groups, the Spanish Y-chromosome contribution was much more marked than that estimated using mtDNA. This analysis showed a usual trend in the Spanish Colonial history, characterized by a demographic collapse of the aboriginal population, but with considerable introgression of genes through native women. In accordance to D....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gran Canaria; Y-chromosome polymorphisms; Admixture.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100001

Transference of microsatellite markers from Eucalyptus spp to Acca sellowiana and the successful use of this technique in genetic characterization
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Karine Louise dos; Welter,Leocir José; Dantas,Adriana Cibele de Mesquita; Guerra,Miguel Pedro; Ducroquet,Jean Pierre Henri Joseph; Nodari,Rubens Onofre.
The pineapple guava (Acca sellowiana), known in portuguese as the goiabeira-serrana or "Feijoa", is a native fruit tree from southern Brazil and northern Uruguay that has commercial potential due to the quality and unique flavor of its fruits. Knowledge of genetic variability is an important tool in various steps of a breeding program, which can be facilitated by the use of molecular markers. The conservation of repeated sequences among related species permits the transferability of microsatellite markers from Eucalyptus spp. to A. sellowiana for testing. We used primers developed for Eucalyptus to characterize A. sellowiana accessions. Out of 404 primers tested, 180 amplified visible products and 38 were polymorphic. A total of 48 alleles were detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feijoa sellowiana; Genetic diversity; Goiabeira-serrana; Pineapple-guava; Transferability.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100014

Identification of the soybean HyPRP family and specific gene response to Asian soybean rust disease
Provedor de dados: Genetics and Molecular Biology
Autores: Bücker Neto,Lauro; Oliveira,Rafael Rodrigues de; Wiebke-Strohm,Beatriz; Bencke,Marta; Weber,Ricardo Luís Mayer; Cabreira,Caroline; Abdelnoor,Ricardo Vilela; Marcelino,Francismar Correa; Zanettini,Maria Helena Bodanese; Passaglia,Luciane Maria Pereira.
Soybean [Glycine max (L.) Merril], one of the most important crop species in the world, is very susceptible to abiotic and biotic stress. Soybean plants have developed a variety of molecular mechanisms that help them survive stressful conditions. Hybrid proline-rich proteins (HyPRPs) constitute a family of cell-wall proteins with a variable N-terminal domain and conserved C-terminal domain that is phylogenetically related to non-specific lipid transfer proteins. Members of the HyPRP family are involved in basic cellular processes and their expression and activity are modulated by environmental factors. In this study, microarray analysis and real time RT-qPCR were used to identify putative HyPRP genes in the soybean genome and to assess their expression in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungal disease; HyPRP genes; Glycine max; Real time RT-qPCR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200012

Cloning of a chitinase gene from Ewingella americana, a pathogen of the cultivated mushroom, Agaricus bisporus
Provedor de dados: Genetics and Molecular Biology
Autores: Inglis,P.W.; Peberdy,J.F.; Sockett,R.E.
We have isolated a gene encoding a chitinase (EC 3.2.1.14) from Ewingella americana, a recently described pathogen of the mushroom Agaricus bisporus. This gene, designated chiA (EMBL/Genbank/DDBJ accession number X90562), was cloned by expression screening of a plasmid-based E. americana HindIII genomic library in Escherichia coli using remazol brilliant violet-stained carboxymethylated chitin incorporated into selective medium. The chiA gene has a 918-bp ORF, terminated by a TAA codon, with a calculated polypeptide size of 33.2 kDa, likely corresponding to a previously purified and characterised 33-kDa endochitinase from E. americana. The deduced amino acid sequence shares 33% identity with chitinase II from Aeromonas sp. No. 10S-24 and 7.8% identity with...
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300030

Clinical and molecular characteristics of sickle cell anemia in the northeast of Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Adorno,Elisângela Vitória; Zanette,Ângela; Lyra,Isa; Seixas,Magda Oliveira; Reis,Mitermayer Galvão; Gonçalves,Marilda Souza.
Beta S-globin gene (βS-globin) haplotypes, markers for severe sickle cell anemia (SCA), and the alpha-thalassemia 2 gene 3.7 kb deletion (-α2(3.7 kb) thal) along with demographic and clinical data were investigated in SCA outpatients (n = 125, 63 female and 62 male) in the Brazilian state of Bahia, which has a high prevalence SCA. PCR-RFLP showed that the Central African Republic/Benin (CAR/BEN, 51.2%) haplotype was most frequent, followed by the Benin/Benin (Ben/Ben, 28.8%). At least one CAR haplotype was present in every outpatient with a history of cerebrovascular accident. The Cameroon (Cam), Senegal (Sen) and Arab-India haplotypes occurred in small numbers, as did atypical haplotypes. Fetal hemoglobin (HbF, %) was unevenly distributed. Compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-thalassemia 2 gene 3.7 kb deletion (-α23.7 kb thal); ΒS-globin gene haplotypes; Fetal hemoglobin.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400003

Identification of sugarcane genes involved in the purine synthesis pathway
Provedor de dados: Genetics and Molecular Biology
Autores: Jancso,Mario A.; Sculaccio,Susana A.; Thiemann,Otavio H.
Nucleotide synthesis is of central importance to all cells. In most organisms, the purine nucleotides are synthesized de novo from non-nucleotide precursors such as amino acids, ammonia and carbon dioxide. An understanding of the enzymes involved in sugarcane purine synthesis opens the possibility of using these enzymes as targets for chemicals which may be effective in combating phytopathogen. Such an approach has already been applied to several parasites and types of cancer. The strategy described in this paper was applied to identify sugarcane clusters for each step of the de novo purine synthesis pathway. Representative sequences of this pathway were chosen from the National Center for Biotechnology Information (NCBI) database and used to search the...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100033

Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide
Provedor de dados: Genetics and Molecular Biology
Autores: Becker,Tânia C.A.; Chiuchetta,Simone J.R.; Baptista,Francielle; Castro-Prado,Marialba A.A. de.
Ethidium bromide (EB) is an intercalating inhibitor of topoisomerase II and its activities are related to chemotherapeutic drugs used in anti-cancer treatments. EB promotes several genotoxic effects in exposed cells by stabilising the DNA-enzyme complex. The recombinagenic potential of EB was evaluated in our in vivo study by the loss of heterozygosity of nutritional markers in diploid Aspergillus nidulans cells through Homozygotization Index (HI). A DNA repair mutation, uvsZ and a chromosome duplication DP (II-I) were introduced in the genome of tested cells to obtain a sensitive system for the recombinagenesis detection. EB-treated diploid cells had HI values significantly greater than the control at both concentrations (4.0 x 10-3 and 5.0 x 10-3 mM)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antineoplastic drug; Aspergillus nidulans; Ethidium bromide; Intercalating agent; Mitotic crossing over.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300026

microRNAs and the mammary gland: a new understanding of gene expression
Provedor de dados: Genetics and Molecular Biology
Autores: Gigli,Isabel; Maizon,Daniel Omar.
MicroRNAs (miRNAs) have been identified in cells as well as in exosomes in biological fluids such as milk. In mammary gland, most of the miRNAs studied have functions related to immunity and show alterations in their pattern of expression during lactation. In mastitis, the inflammatory response caused by Streptococcus uberis alters the expression of miRNAs that may regulate the innate immune system. These small RNAs are stable at room temperature and are resistant to repeated freeze/thaw cycles, acidic conditions and degradation by RNAse, making them resistant to industrial procedures. These properties mean that miRNAs could have multiple applications in veterinary medicine and biotechnology. Indeed, lactoglobulin-free milk has been produced in transgenic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactation; Mastitis; Milk; MiRNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400001

Newton Freire-Maia (1918-2003): from Boa Esperança to the world
Provedor de dados: Genetics and Molecular Biology
Autores: Salzano,Francisco M.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300001

Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogaster
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Wender Ferreira; Oliveira,Alaide Braga de; Nepomuceno,Júlio César.
This study investigated the genotoxicity of Lapachol (LAP) evaluated by wing spot test of Drosophila melanogaster in the descendants from standard (ST) and high bioactivation (HB) crosses. This assay detects the loss of heterozygosity of marker genes expressed phenotypically on the fly's wings. Drosophila has extensive genetic homology to mammals, which makes it a suitable model organism for genotoxic investigations. Three-day-old larvae from ST crosses (females flr³/TM3, Bd s x males mwh/mwh), with basal levels of the cytochrome P450 and larvae of high metabolic bioactivity capacity (HB cross) (females ORR; flr³/TM3, Bd s x males mwh/mwh), were used. The results showed that LAP is a promutagen, exhibiting genotoxic activity in larvae from the HB cross. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila melanogaster; SMART; Lapachol; Doxorubicin; Cytochrome P450.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300029

Inheritance of resistance to Phytophthora infestans (Peronosporales, Pythiaceae) in a new source of resistance in tomato (Solanum sp. (formerly Lycopersicon sp.), Solanales, Solanaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Abreu,Flávia Barbosa; Silva,Derly José Henriques da; Cruz,Cosme Damião; Mizubuti,Eduardo Seiti Gomide.
In Brazil, no commercial tomato (Solanum lycopersicum L. formerly Lycopersicon esculentum Mill.) varieties are available which are resistant to the late blight, one of the most important tomato diseases, produced by the phytopathogenic oomycete Phytophthora infestans. The wild tomato (Solanum habrochaites Knapp &amp;amp; Spooner, formerly Lycopersicon hirsutum Dunal) shows resistance to P. infestans, because of which we investigated an interspecific cross between S. lycopersicum cv. Santa Clara and S. habrochaites accession BGH 6902 maintained at the Horticultural Germplasm Bank at the Federal University of Viçosa (Banco de Germoplasma de Horticultura (BGH), Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil) The genitors, F1, F2, BC1 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Late blight; Lycopersicon esculentum; Lycopersicon hirsutum; Solanum lycopersicum; Solanum habrochaites.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300016

Combined GSTM1 and GSTT1 null genotypes are strong risk factors for atherogenesis in a Serbian population
Provedor de dados: Genetics and Molecular Biology
Autores: Grubisa,Ivana; Otasevic,Petar; Vucinic,Nada; Milicic,Biljana; Jozic,Tanja; Krstic,Slobodan; Milasin,Jelena.
Abstract Oxidative stress (OS) plays an important role in atherogenesis and since glutathione S-transferases (GSTs) provide protection against OS, we have tested the hypothesis that deletion polymorphisms in two GSTs (GSTM1 and GSTT1) may affect the risk of developing atherosclerosis. A total of 382 individuals (200 patients with atherosclerosis and 182 healthy controls) were included in this association study. Genomic DNA was isolated from peripheral blood cells or from buccal epithelial cells and genotyping was performed using multiplex-PCR or real-time PCR methods. GSTM1 null genotype was significantly more frequent in atherosclerotic patients than in controls (52.0% vs 34.1%) and individuals with the GSTM1 null genotype had an approximately 2-fold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Gene polymorphisms; Glutathione S-transferases; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100035

A new karyotype for the genus Cavia from a southern island of Brazil (Rodentia - Caviidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Gava,A.; Freitas,T.R.O.; Olimpio,J.
Intraspecific karyotype variation in mammal species is very common and often caused by centromeric fusion of acrocentric chromosomes. We describe here a new karyotype 2n = 62 (FN = 112) for the genus Cavia from the Moleques do Sul Islands, of the southern coast of Brazil. We analyzed two male and four female karyotypes that had twenty-four biarmed pairs and six pairs of acrocentric chromosomes. The sexual pair consisted of a metacentric X-chromosome and a large acrocentric Y. C-bands were found in the centromeric and pericentromeric regions of almost all chromosomes, except for some small biarmed and acrocentric ones. Nucleolus organizer regions appeared in two biarmed chromosomes, and G-banding patterns were also seen.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100013

A tribute to José María ("Chema") Cantú
Provedor de dados: Genetics and Molecular Biology
Autores: Penchaszadeh,Victor B.; Rojas-Martinez,Augusto; Llerena,Adrián.
José María ("Chema") Cantú (1938-2007), born in Mexico, was a pioneering, loved and respected leader in medical and human genetics and bioethics in Latin America. He graduated as a physician in Mexico and then trained in medical and human genetics in France and the United States. He was instrumental in developing a first-rate research, training and genetic services program in medical and human genetics in Guadalajara, in northwestern Mexico. He acted forcefully at national, regional and international levels to promote scientific development through collaboration and education in science and humanities, while he simultaneously strived for justice, peace, love and human rights. He attained some of the highest honors a scientist and humanist could aspire to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cantú José María; Medical genetics; Human genetics; Bioethics; Latin America.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200018

Pollen and seed flow patterns of Carapa guianensis Aublet. (Meliaceae) in two types of Amazonian forest
Provedor de dados: Genetics and Molecular Biology
Autores: Martins,Karina; Raposo,Andréa; Klimas,Christie A; Veasey,Elizabeth A; Kainer,Karen; Wadt,Lúcia Helena O.
Various factors affect spatial genetic structure in plant populations, including adult density and primary and secondary seed dispersal mechanisms. We evaluated pollen and seed dispersal distances and spatial genetic structure of Carapa guianensis Aublet. (Meliaceae) in occasionally inundated and terra firme forest environments that differed in tree densities and secondary seed dispersal agents. We used parentage analysis to obtain contemporary gene flow estimates and assessed the spatial genetic structure of adults and juveniles. Despite the higher density of adults (diameter at breast height ; 25 cm) and spatial aggregation in occasionally inundated forest, the average pollen dispersal distance was similar in both types of forest (195 ± 106min terra...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene flow; Parentage analysis; Scatterhoarding; Seed dispersal; Spatial genetic structure.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500017

Population structure analysis of Pimelodus maculatus (Pisces, Siluriformes) from the Tietê and Paranapanema Rivers (Brazil)
Provedor de dados: Genetics and Molecular Biology
Autores: Almeida,Fernanda Simões de; Sodré,Leda Maria Koelblinger; Contel,Eucleia Primo Betioli.
Pimelodus maculatus populations from the Tietê and Paranapanema rivers were sampled and had their genetic structure analyzed by using RAPD markers, with the aim of contributing to future conservation studies. The proportion of polymorphic loci was greater than 50% in the populations of both rivers. Genetic diversity data showed that, in spite of its nine hydroelectric plants, the Tietê river population was genetically homogeneous, whereas the Paranapanema river population was structured. This might be due to the presence of high waterfalls distributed all along its course. These data may serve as indicators for future conservation studies on the Tietê and Paranapanema rivers.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RAPD markers; Population structure; Conservation; Genetic variability; Pimelodus.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300014

Analysis of the DMPK gene CTG repeat in healthy Brazilians
Provedor de dados: Genetics and Molecular Biology
Autores: Freitas,Silvia Regina Sampaio; Cabello,Pedro H.; Moura-Neto,Rodrigo Soares; Duro,Luiz Alves.
Myotonic dystrophy (DM) is a neuromuscular disorder caused by the expansion of the cytosine-thymine-guanine (CTG) repeat of the myotonic dystrophy protein kinase gene (DMPK). This repeat is highly polymorphic in healthy individuals [(CTG)5-37], and it has been proposed that expanded CTG alleles originated from larger sized normal alleles [(CTG)19-37]. According to this hypothesis, a positive correlation should be expected between the frequency of these large-sized normal alleles and the prevalence of the disorder in a population. We determined the distribution of CTG alleles of the DMPK gene in 156 healthy Brazilians from Rio de Janeiro city. Our analyses of 312 chromosomes detected 20 different alleles ranging in size from 5 to 27 CTG repeats, with 24...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myotonic dystrophy; Dynamic mutation; Trinucleotide repeat; CTG repeat; DMPK gene.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100004

The diversity of citrus endophytic bacteria and their interactions with Xylella fastidiosa and host plants
Provedor de dados: Genetics and Molecular Biology
Autores: Azevedo,João Lúcio; Araújo,Welington Luiz; Lacava,Paulo Teixeira.
Abstract The bacterium Xylella fastidiosa is the causal agent of citrus variegated chlorosis (CVC) and has been associated with important losses in commercial orchards of all sweet orange [Citrus sinensis (L.)] cultivars. The development of this disease depends on the environmental conditions, including the endophytic microbial community associated with the host plant. Previous studies have shown that X. fastidiosa interacts with the endophytic community in xylem vessels as well as in the insect vector, resulting in a lower bacterial population and reduced CVC symptoms. The citrus endophytic bacterium Methylobacterium mesophilicum can trigger X. fastidiosa response in vitro, which results in reduced growth and induction of genes associated with energy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytes; Citrus sinensis; Curtobacterium flaccumfaciens; Methylobacterium mesophilicum; Symbiotic control.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400476

Overexpression of salt-induced protein (salT) delays leaf senescence in rice
Provedor de dados: Genetics and Molecular Biology
Autores: Zhu,Keming; Tao,Huimin; Xu,Shuo; Li,Kaixia; Zafar,Sundus; Cao,Wei; Yang,Yanhua.
Abstract Senescence, a highly programmed process, largely determines yield and quality of crops. However, knowledge about the onset and progression of leaf senescence in crop plants is still limited. Here, we report that salt-induced protein (salT), a new gene, may be involved in leaf senescence. Overexpressing salT could prolong the duration of leaves with higher concentrations of chlorophyll compared with the wild type. Moreover, overexpression of salT could delay the senescence of rice leaves though the inhibition of senescence associated genes (SAGs). Overall, the characterization of salT suggested that it is a new gene affecting the leaf senescence induced by natural and dark conditions.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leaf senescence; Salt-induced protein (salT); Rice; Stay-green.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100080

Genetic analysis of scattered populations of the Indian eri silkworm, Samia cynthia ricini Donovan: differentiation of subpopulations
Provedor de dados: Genetics and Molecular Biology
Autores: Pradeep,Appukuttannair R.; Jingade,Anuradha H.; Singh,Choba K.; Awasthi,Aravind K.; Kumar,Vikas; Rao,Guruprasad C.; Prakash,N.B. Vijaya.
Deforestation and exploitation has led to the fragmentation of habitats and scattering of populations of the economically important eri silkworm, Samia cynthia ricini, in north-east India. Genetic analysis of 15 eri populations, using ISSR markers, showed 98% inter-population, and 23% to 58% intra-population polymorphism. Nei's genetic distance between populations increased significantly with altitude (R² = 0.71) and geographic distance (R² = 0.78). On the dendrogram, the lower and upper Assam populations were clustered separately, with intermediate grouping of those from Barpathar and Chuchuyimlang, consistent with geographical distribution. The Nei's gene diversity index was 0.350 in total populations and 0.121 in subpopulations. The genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Subpopulation differentiation; Scattered population; Samia cynthia ricini; ISSR loci; Gene diversity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300022

Editorial commentary on the state of clinical genetics in Brazil exemplified by the article on prenatal diagnosis by Kessler et al. Genetics and Molecular Biology (this issue)
Provedor de dados: Genetics and Molecular Biology
Autores: Pearson,Peter.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500005

Mechanisms of sugarcane response to herbivory
Provedor de dados: Genetics and Molecular Biology
Autores: Falco,Maria Cristina; Marbach,Phellippe Arthur S.; Pompermayer,Patrícia; Lopes,Francisco Cláudio C.; Silva-Filho,Marcio C.
Deciphering plant-insect interactions at the molecular level is one of the major topics of interest in contemporary plant biology research. In the last few years, various aspects of the plant response to insect damage have been investigated, including the characterization of direct and indirect responses, the regulation of gene expression resulting from insect attack and the signal transduction pathways. Such research has resulted in the proposal of new methods to enhance host resistance to insect pests, including the use of insecticidal genes that can be transferred by genetic engineering into target crops. By integrating the understanding of how plants react to insect damage with the techniques of molecular biology researchers should be able to increase...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100016

Neonatal screening for four lysosomal storage diseases with a digital microfluidics platform: Initial results in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Camargo Neto,Eurico; Schulte,Jaqueline; Pereira,Jamile; Bravo,Heydy; Sampaio-Filho,Claudio; Giugliani,Roberto.
Abstract We describe the initial results of a neonatal screening program for four lysosomal storage diseases (MPS I, Pompe, Gaucher and Fabry) using the digital microfluidics methodology. The method successfully identified patients previously diagnosed with these diseases and was used to test dried blood spot samples obtained from 10,527 newborns aged 2 to 14 days. The digital microfluidic technology shows potential for a simple, rapid and high-throughput screening for these four diseases in a standard neonatal screening laboratory.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lysossomal storage diseases; Neonatal screening; Digital microfluidics; Brazil.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300414

Cell fusion and chromatin degeneration in an inbred line of maize
Provedor de dados: Genetics and Molecular Biology
Autores: Caetano-Pereira,Creuci Maria; Pagliarini,Maria Suely; Brasil,Edward Madureira.
A large number of maize genotypes are currently being evaluated to determine the effect of environmental stress caused by the aluminum found in cerrado soils on meiotic behavior. In the present study we report the occurrence of cell fusion followed or not by chromatin degeneration in meiocytes of some plants of an inbred line of maize grown on acid and corrected soil in the Brazilian central plateau. The results suggest the absence of any soil effect on the irregularities observed since the number of affected plants was higher among those grown on corrected soil. The possible causes of these irregularities and their influence on male sterility are discussed.
Tipo: Info:eu-repo/semantics/other
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100014

Chromosome identification in the Andean common bean accession G19833 (Phaseolus vulgaris L., Fabaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Altrock,Sarah; Fonsêca,Artur; Pedrosa-Harand,Andrea.
Characterization of all chromosomes of the Andean G19833 bean genotype was carried out by fluorescent in situ hybridization. Eleven single-copy genomic sequences, one for each chromosome, two BACs containing subtelomeric and pericentromeric repeats and the 5S and 45S ribosomal DNA (rDNA) were used as probes. Comparison to the Mesoamerican accession BAT93 showed little divergence, except for additional 45S rDNA sites in four chromosome pairs. Altogether, the results indicated a relative karyotypic stability during the evolution of the Andean and Mesoamerican gene pools of P. vulgaris.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Common bean; Repetitive DNA; Chromosome marker; Cytogenetic map; Comparative map; Intraspecific variation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300016

Genetic trends in the performance and reproductive traits of pigs
Provedor de dados: Genetics and Molecular Biology
Autores: Torres Filho,Rodolpho de Almeida; Torres,Robledo de Almeida; Lopes,Paulo Sávio; Pereira,Carmen Silva; Euclydes,Ricardo Frederico; Araújo,Cláudio Vieira de; Silva,Martinho de Almeida e.
The selection and evaluation of breeds and purebred lines to be used as the parental stock is an important step for the success of any animal breeding program. In this study, the feed:gain ratio, backfat thickness corrected for 100 kg, age to 100 kg, average daily gain, and age of sow at first farrowing were used to evaluate the genetic trends for direct and maternal additive genetic values in Large White pigs. The total number of piglets born or born alive and the litter weight at birth were used as indicators of the genetic trends for the direct additive values. The genetic trends were calculated by regression of the average predicted genetic values per year for each trait versus the offspring's year of birth (for performance traits) or versus the dam...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Average daily gain; Backfat thickness; Birth litter weight.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100017

Birth weight of twins: 2. Fetal genetic effect on birth weight
Provedor de dados: Genetics and Molecular Biology
Autores: Beiguelman,Bernardo; Colletto,Glória M.D.D.; Franchi-Pinto,Carla; Krieger,Henrique.
Intraclass correlation coefficients were calculated for the birth weights of twins born at three southeastern Brazilian hospitals, after adjustment of the natural logarithms of these weights for gestational age, its quadratic and cubic terms, sex, and their interactions. The data indicate that fetal genetic effect on birth weight might have the opportunity to be demonstrated by children born to undernourished women. Undernourishment, acting as a selective force, might enhance the existence of genotypes that determine less need of food for normal development.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100026

Erratum
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300552

Molecular Cytogenetics II: PCR-based diagnosis of chromosomal deletions and microdeletion syndromes
Provedor de dados: Genetics and Molecular Biology
Autores: Pena,Sérgio D.J.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400008

Glutathione S-transferase Mu (GSTM1) null genotype in relation to gender, age and smoking status in a healthy Brazilian population
Provedor de dados: Genetics and Molecular Biology
Autores: Losi-Guembarovski,Roberta; D’Arce,Luciana Paula Grégio; Cólus,Ilce Mara de Syllos.
The glutathione S-transferase mu (GSTM1) gene which acts during phase II of xenobiotic metabolism is polymorphic in the population, being absent in about 30-50% of individuals depending on the ethnic group from which they come. Epidemiological studies suggest that individuals who are homozygous null at the GSTM1 locus may have an increased risk of developing various types of neoplastic disease. We used the polymerase chain reaction (PCR) to estimate the frequency of GSTM1 in 176 healthy individuals from the north of Paraná (Brazilian state), the null genotype being detected in 48.86% of these individuals. The Student’s t-test was used to evaluate the frequency of the glutathione S-transferase null genotype in relation to age, gender and smoking habit and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione S-transferase Mu (GSTM1); Control individuals; Brazilian population; Age; Smoking habit.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400001

Genetic variability in the Skyros pony and its relationship with other Greek and foreign horse breeds
Provedor de dados: Genetics and Molecular Biology
Autores: Bömcke,Elisabeth; Gengler,Nicolas; Cothran,E. Gus.
In Greece, seven native horse breeds have been identified so far. Among these, the Skyros pony is outstanding through having a distinct phenotype. In the present study, the aim was to assess genetic diversity in this breed, by using different types of genetic loci and available genealogical information. Its relationships with the other Greek, as well as foreign, domestic breeds were also investigated. Through microsatellite and pedigree analysis it appeared that the Skyros presented a similar level of genetic diversity to the other European breeds. Nevertheless, comparisons between DNA-based and pedigree-based results revealed that a loss of genetic diversity had probably already occurred before the beginning of breed registration. Tests indicated the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variation; Genetic markers; Pedigree; Horse.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100013

Inbreeding depression of 28 maize elite open pollinated varieties
Provedor de dados: Genetics and Molecular Biology
Autores: Pacheco,Cleso Antônio Patto; Santos,Manoel Xavier dos; Cruz,Cosme Damião; Parentoni,Sidney Netto; Guimarães,Paulo Evaristo de Oliveira; Gama,Elto Eugênio Gomes e; Silva,Álvaro Eleutério da; Carvalho,Hélio Wilson Lemos de; Vieira Júnior,Pedro Abel.
The study of inbreeding depression is important for breeding strategies such as use of inbred progenies or extraction of inbreed lines. A diallel of 28 maize open-pollinated varieties was evaluated in 10 environments in the early 1990s. At the same time, S1 populations for each of the 28 varieties were evaluated in the same 10 experiments (environments). Yield reductions of the populations from S0 to S1 (mean of the 10 environments), varied from 34.6% (CMS-01) to 59.2% (CMS-30), with an average of 49.1%. Inbreeding depression was greater in populations with a wider genetic base, which had never been exposed to inbreeding (CMS-30, BR-107, PH4, Cunha, Saracura, Nitrodent, and Nitroflint). Inbred lines with greater yield means should be obtained from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inbreeding depression; Endogamy; Maize; Breeding; Diallel.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400014

Reproductive deficiency in bulls from synthetic breeds according to the type of crossbreed and the morphology of the Y chromosome
Provedor de dados: Genetics and Molecular Biology
Autores: Horn,Marilise M.; Moraes,José C.F.; Jaume,Carlos M.; Edelweiss,Maria I.A.; Rosado,Adriano.
We used 550 Braford and 214 Brangus-Ibagé bulls to study the association between Y chromosome morphology and the evaluation of breeding-soundness, karyotyping and breeding records were used to assess Y chromosome morphology. In general, no direct association was detected between the individual type of Y chromosome and reproductive fitness as previously estimated through breeding soundness examination. The type of breeding strategy used to form the synthetic breeds was also analyzed. The Braford breed is about 3/8 Zebu (Bos taurus indicus) and 5/8 Hereford (Bos taurus taurus) but when the breeding strategy used 1/4 Zebu animals to produce Braford bulls which were 3/8 Zebu a greater percentage of the bulls had to be culled due to reproductive problems. Our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bulls; Semen quality; Y chromosome; Synthetic breeds of cattle.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200008

Distribution of PLD and FagA, B, C and D genes in Corynebacterium pseudotuberculosis isolates from sheep and goats with caseus lymphadenitis
Provedor de dados: Genetics and Molecular Biology
Autores: Sá,Maria da Conceição Aquino de; Gouveia,Gisele Veneroni; Krewer,Carina da Costa; Veschi,Josir Laine Aparecida; Mattos-Guaraldi,Ana Luiza de; Costa,Mateus Matiuzzi da.
Caseous lymphadenits (CL) is a chronic and subclinical disease that affects goats and sheep and, consequently, causes economic losses, especially to small producers. The purpose of this study, through use of Polymerase Chain Reaction (PCR), was to verify the presence of virulence genes of phospholipase D (PLD), integral membrane protein (FagA), iron enterobactin transporter (FagB), ATP binding cytoplasmic membrane protein (FagC) and iron siderophore binding protein (FagD) in 168 isolates of C. pseudotuberculosis obtained from cases of caseous lymphadenitis in goats and sheep. FagA, FagB and PLD genes were detected in all 145 strains isolated from abscesses in superficial lymph nodes and in 23 strains isolated from viscera. The FagC gene was positive in 167...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Virulence genes; C. pseudotuberculosis; Caseous lymphadenits.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200017

Eucalyptus urograndis stem proteome is responsive to short-term cold stress
Provedor de dados: Genetics and Molecular Biology
Autores: Leonardi,Gabriela de Almeida; Carlos,Natália Aparecida; Mazzafera,Paulo; Balbuena,Tiago Santana.
Eucalyptus urograndis is a hybrid eucalyptus of major economic importance to the Brazilian pulp and paper industry. Although widely used in forest nurseries around the country, little is known about the biochemical changes imposed by environmental stress in this species. In this study, we evaluated the changes in the stem proteome after short-term stimulation by exposure to low temperature. Using two-dimensional gel electrophoresis coupled to high-resolution mass spectrometry-based protein identification, 12 proteins were found to be differentially regulated and successfully identified after stringent database searches against a protein database from a closely related species (Eucalyptus grandis). The identification of these proteins indicated that the E....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic stress; Mass spectrometry; Omics; Proteomics.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191

A viable fetus presenting 68,XX[73]/69,XXX[27] triploid mosaicism
Provedor de dados: Genetics and Molecular Biology
Autores: Acosta,A.X.; Peres,L.C.; Mazzucatto,L.F.; Pina-Neto,J.M.
Triploidy is common in human pregnancies. It is detected in 1 to 2% of clinically recognized pregnancies and in approximately 15 to 20% of spontaneous abortions produced by chromosome anomalies. We report a premature liveborn girl (30 weeks of gestation) with microcephaly, facial dysmorphism and skeletal abnormalities who died at one day of age due to respiratory failure. The placenta showed partial hydatiform mole. Autopsy revealed no internal malformations. Cytogenetic analysis of 100 metaphases obtained from renal tissue culture revealed a 68,XX[73]/69,XXX[27] karyotype. To our knowledge this is the first report in the literature of 68,XX[73]/69,XXX[27] mosaicism in a liveborn infant.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300002

Genetic hearing loss: a study of 228 Brazilian patients
Provedor de dados: Genetics and Molecular Biology
Autores: Longhitano,Silvia Bragagnolo; Brunoni,Décio.
We studied 228 patients, with suspected or confirmed genetic hearing loss, in order to determine the clinical and genetic diagnoses and etiology of each case. Deafness with no associated abnormalities was found in 146 patients (64%) belonging to 112 families. Syndromic deafness was diagnosed in 82 patients (36%) belonging to 76 families. The genetic etiology was as follows: autosomal recessive inheritance in 40.8% of syndromics and non-syndromics, autosomal dominant inheritance in 13.2% and X-linked recessive in 1.3%. In 44.7% of the cases, the etiology of the hearing loss could not be determined. Monogenic causes are the most possible etiology in the latter cases. Parental consanguinity was found in 22.4% of the cases, and deafness was bilateral, profound...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100004

Evolution of DNMT2 in drosophilids: Evidence for positive and purifying selection and insights into new protein (pathways) interactions
Provedor de dados: Genetics and Molecular Biology
Autores: Vieira,Gilberto Cavalheiro; D’Ávila,Marícia Fantinel; Zanini,Rebeca; Deprá,Maríndia; da Silva Valente,Vera Lúcia.
Abstract The DNA methyltransferase 2 (DNMT2) protein is the most conserved member of the DNA methyltransferase family. Nevertheless, its substrate specificity is still controversial and elusive. The genomic role and determinants of DNA methylation are poorly understood in invertebrates, and several mechanisms and associations are suggested. In Drosophila, the only known DNMT gene is Dnmt2. Here we present our findings from a wide search for Dnmt2 homologs in 68 species of Drosophilidae. We investigated its molecular evolution, and in our phylogenetic analyses the main clades of Drosophilidae species were recovered. We tested whether the Dnmt2 has evolved neutrally or under positive selection along the subgenera Drosophila and Sophophora and investigated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila; Dnmt2; Positive selection; Epigenetic; Positive-destabilizing selection.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200215

First cytogenetic characterization of the sub-arctic marine fish Mallotus villosus (Müller, 1776), Osmeriformes, Osmeridae
Provedor de dados: Genetics and Molecular Biology
Autores: Ghigliotti,Laura; Mazzei,Federico; Ozouf-Costaz,Catherine; Christiansen,Jørgen S.; Fevolden,Svein-Erik; Pisano,Eva.
The capelin, Mallotus villosus (Osmeriformes, Osmeridae), is an ecological and commercial key component of the sub-arctic ichthyofauna. Here, we provide the first cytogenetic information on the species based on both conventional karyotyping and chromosomal mapping of 45S and 5S ribosomal genes through fluorescence in situ hybridization (FISH). The capelin genome displayed a diploid number of 54 with the karyotypic formula 26m/sm+28st/a and a fundamental number (FN) = 80. Both classes of ribosomal genes appeared to be spread out to multiple chromosomal locations, i.e. the 45S and 5S rDNA clusters were detected on six and seven chromosome pairs, respectively. A linked chromosomal organization of the major and minor ribosomal genes classes has been visualized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capelin; Chromosomes; Osmeridae; Ribosomal genes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200003

Identification of resistant genotypes considering polygenic systems in host-pathogen interaction
Provedor de dados: Genetics and Molecular Biology
Autores: Melo,Leonardo Cunha; Santos,João Bosco dos.
Plant resistance to pathogens is the most efficient form of disease control. However, identification of resistant genotypes is often difficult, especially when the genetic basis for the host-pathogen relationship is unknown. This study was carried out to test a methodology capable of providing, in a simple way, information about host vertical and horizontal resistance as well as pathogen virulence. A simulation using twenty, ten, and five pathogen races and twenty hosts was carried out. Host reaction was controlled by ten genes with two alleles each. Eight genes had little effects, one had medium and the other strong effects. Genetic control of pathogenicity was identical to that of the host. Only homozygous genotypes were used for pathogens and hosts in...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400022

Geographic polymorphism of P element in populations of Drosophila sturtevanti
Provedor de dados: Genetics and Molecular Biology
Autores: Almeida,Luciane M. de; Langeani,Francisco; Carareto,Claudia M.A.
The aim of this report was to detect full-sized P element sequences in eight strains of Drosophila sturtevanti populations from distant geographic regions and to assess the structural geographic variation among P element sequences. PCR analysis confirmed the presence of a putative complete P element in all strains. Southern blot analysis indicated bands shared by all strains, and bands restricted to geographically related strains. Parsimony analysis corroborated the hybridization pattern that reflected the geographic relationships.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canonical P elements; Drosophila; P element polymorphism; Saltans group; Transposable elements.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200010

Karyotype, C-and fluorescence banding pattern, NOR location and FISH study of five Scarabaeidae (Coleoptera) species
Provedor de dados: Genetics and Molecular Biology
Autores: Bione,Edgar; Moura,Rita de Cássia de; Carvalho,Reginaldo de; Souza,Maria José de.
Meiotic chromosomes obtained from members of the coleopteran subfamilies Rutelinae and Dynastinae were studied using standard and silver nitrate staining, C-banding, base-specific fluorochromes and fluorescent in situ hybridization (FISH). The study presents detailed karyotipic descripitions of three Rutelinae species (Geniates borelli, Macraspis festiva and Pelidnota pallidipennis), and two Dynastinae species (Lygirus ebenus and Strategus surinamensis hirtus) with special emphasis on the distribution and variability of constitutive heterochromatin and the nucleolar organizer region (NOR). We found that for G. borelli, P. pallidipennis, L. ebenus and S. s hirtus the karyotype was 2n = 20 (9II + Xy p), with G. borelli, P. pallidipennis and L. ebenus showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Constitutive heterochromatin; NORs; RDNA sequences.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300006

Comparison of similarity coefficients used for cluster analysis with dominant markers in maize (Zea mays L)
Provedor de dados: Genetics and Molecular Biology
Autores: Meyer,Andréia da Silva; Garcia,Antonio Augusto Franco; Souza,Anete Pereira de; Souza Jr.,Cláudio Lopes de.
The objective of this study was to evaluate whether different similarity coefficients used with dominant markers can influence the results of cluster analysis, using eighteen inbred lines of maize from two different populations, BR-105 and BR-106. These were analyzed by AFLP and RAPD markers and eight similarity coefficients were calculated: Jaccard, Sorensen-Dice, Anderberg, Ochiai, Simple-matching, Rogers and Tanimoto, Ochiai II and Russel and Rao. The similarity matrices obtained were compared by the Spearman correlation, cluster analysis with dendrograms (UPGMA, WPGMA, Single Linkage, Complete Linkage and Neighbour-Joining methods), the consensus fork index between all pairs of dendrograms, groups obtained through the Tocher optimization procedure and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic divergence; Heterotic groups; AFLP; RAPD; Multivariate analysis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100014

Genetic diversity in cultivated carioca common beans based on molecular marker analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Perseguini,Juliana Morini Küpper Cardoso; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Colombo,Carlos Augusto; Carbonell,Sérgio Augusto Moraes; Mondego,Jorge Mauricio Costa; Gazaffi,Rodrigo; Garcia,Antonio Augusto Franco; Campos,Tatiana de; Souza,Anete Pereira de; Rubiano,Luciana Benchimol.
A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLPs; Genetic structure; Genetic variability; Phaseolus vulgaris L.; SSRs.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100017

Mutations in retrotransposon AtCOPIA4 compromises resistance to Hyaloperonospora parasitica in Arabidopsis thaliana
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,Yi-Hong; Warren Jr.,James T.
Retrotransposons (RTEs) are a principal component of most eukaryotic genomes, representing 50%-80% of some grass genomes. RTE sequences have been shown to be preferentially present in disease resistance gene clusters in plants. Arabidopsis thaliana has over 1,600 annotated RTE sequences and 56 of these appear to be expressed because of the exact expressed sequence tag (EST) matches and the presence of intact open reading frames. Of the 22 represented in the Affymetrix ATH1 array, AtCOPIA4 was found to be expressed at a higher level than all other RTEs across different developmental stages. Since AtCOPIA4 is located in the RPP5 gene cluster and is adjacent to RPP4 which confers resistance to the downy mildew oomycete Hyaloperonospora parasitica isolate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arabidopsis thaliana; Retrotransposon; Downy mildew resistance; Knockout.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100023

Chromosomal localization of the 18S-28S and 5S rRNA genes and (TTAGGG)n sequences of butterfly lizards (Leiolepis belliana belliana and Leiolepis boehmei, Agamidae, Squamata)
Provedor de dados: Genetics and Molecular Biology
Autores: Srikulnath,Kornsorn; Uno,Yoshinobu; Matsubara,Kazumi; Thongpan,Amara; Suputtitada,Saowanee; Apisitwanich,Somsak; Nishida,Chizuko; Matsuda,Yoichi.
Chromosomal mapping of the butterfly lizards Leiolepis belliana belliana and L. boehmei was done using the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences. The karyotype of L. b. belliana was 2n = 36, whereas that of L. boehmei was 2n = 34. The 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, while the 5S rRNA genes were found in the pericentromeric region of chromosome 6 in both species. Hybridization signals for the (TTAGGG)n sequence were observed at the telomeric ends of all chromosomes, as well as interstitially at the same position as the 18S-28S rRNA genes in L. boehmei. This finding suggests that in L. boehmei telomere-to-telomere fusion probably occurred between chromosome 1 and a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal mapping; FISH; Interstitial telomeric site; Leiolepidinae; Ribosomal RNA gene; Telomeric sequence.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400009

A porcine gene, PBK, differentially expressed in the longissimus muscle from Meishan and Large White pig
Provedor de dados: Genetics and Molecular Biology
Autores: Yonggang,Liu; Sumei,Zhao; Hongbin,Pan; Shizheng,Gao.
An investigation of differences in gene expression in the longissimus muscle of Meishan and Large White pigs was undertaken, using the mRNA display technique. A fragment of one differentially expressed gene was isolated and sequenced, whereupon the complete cDNA sequence was then obtained by using the rapid amplification of cDNA ends (RACE). The nucleotide sequence of the gene is not related to any known porcine gene. Sequence analysis revealed that the open reading frame of this gene encodes a protein with 322 amino acids, thus displaying high sequence identity with the PDZ binding kinase (PBK) of eleven other animal species - dog, horse, cattle, human, chimpanzee, crab-eating macaque, rhesus monkey, rat, mouse, gray short-tailed opossum and platypus, so...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pig; PBK; MRNA differential display; RACE.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400017

Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Lima Júnior,Raimundo Sousa; Scarpassa,Vera Margarete.
Genetic variation was estimated in ten samples populations of Aedes aegypti from the Brazilian Amazon, by using a 380 bp fragment of the mitochocondrial NADH dehydrogenase subunit 4 (ND4) gene. A total of 123 individuals were analyzed, whereby 13 haplotypes were found. Mean genetic diversity was slightly high (h = 0.666 ± 0.029; π = 0.0115 ± 0.0010). Two AMOVA analyses indicated that most of the variation (~70%-72%) occurred within populations. The variation found among and between populations within the groups disclosed lower, but even so, highly significant values. F ST values were not significant in most of the comparisons, except for the samples from Pacaraima and Rio Branco. The isolation by distance (IBD) model was not significant (r = 0.2880; p =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aedes aegypti; Dengue vector; Genetic structure; Gene flow; Brazilian Amazon; Genetic lineages.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200032

Camperdown hemoglobin associated with beta° thalassemia in a Brazilian child
Provedor de dados: Genetics and Molecular Biology
Autores: Tozetto-Mendoza,Tania Regina; Ferreira,Paulo Roberto Santos; Viviani,Nilcéia Maria; Albuquerque,Dulcinéia Martins; Rizzi,Ivana; Araújo,João Targino de.
We report the coexistence of Hb Camperdown [beta104 (G6) Arg -&gt; Ser] and beta°-thalassemia [beta39 (Gln -&gt; stop codon)] in a nine-month-old Brazilian boy. He had a relatively more severe hypochromic and microcytic anemia in comparison to his mother's beta-thalassemia trait. His Hb Camperdown heterozygous father was clinically and hematologically normal. To our knowledge, this is the first description of an association of beta°-thalassemia with Hb Camperdown.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hemoglobin Camperdown; Thalassemia.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300010

Isolation and sequencing of doublesex/male abnormal 3 (DM) related transcription factor (Dmrt) genes from the Asian toad Bufo gargarizans (Cantor, 1842)
Provedor de dados: Genetics and Molecular Biology
Autores: Chen,Wen; Nie,Liu-wang; Zheng,Ping-ping.
The doublesex/male abnormal 3 (dsx/mab-3 or DM) domain gene family involved in sexual development encodes putative transcription factors including a DNA-binding homology motif, the DM domain. We used highly degenerate primers to clone and sequence seven distinct DM related transcription factor (Dmrt) genes from the Asian toad (Bufo gargarizans Cantor, 1842). A database search for the cloned sequences revealed the following percentage identity with the homologous Dmrt genes of the human: BgDmrt1 = 97%, BgDmrt2 = 97%, three isoforms of BgDmrt3 (BgDmrt3a = 93%, BgDmrt3b = 95%, BgDmrt3c = 100%) and two isoforms of BgDmrt5 (BgDmrt5 = 97%, BgDmrt5 = 91%). Based on DM domain amino acid sequence similarities we constructed a phylogenetic tree which grouped...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bufo gargarizans; Dmrt genes; DM domain; SSCP.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600026

Estimates of genetic correlations and correlated responses to selection in cashew (Anacardium occidentale L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Azevedo,Diógenes Manoel Pedroza de; Crisóstomo,João Ribeiro; Almeida,Francisco Célio Guedes; Rossetti,Adroaldo Guimarães.
The present study estimates variances and genetic and phenotypic correlations for five traits in 27 progenies of cashew trees (Anacardium occidentale L.). Data were obtained from a trial conducted in 1992 at Pacajus, Ceará, experimental station of Embrapa Agroindústria Tropical. The characters studied were plant height (PH), North-South and East-West canopy spreads (NSS, EWS), and primary and secondary branch numbers (PBN, SBN). All genetic and phenotypic correlations presented positive and significant values. Selection to increase or decrease the average of any one of the five characteristics of cashew plants in the progenies studied affected the average of the others. The 16-month-old canopy spread can be predicted from NSS or EWS since correlations...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300019

Sex chromosome system ZZ/ZW in Apareiodon hasemani Eigenmann, 1916 (Characiformes, Parodontidae) and a derived chromosomal region
Provedor de dados: Genetics and Molecular Biology
Autores: Bellafronte,Elisangela; Schemberger,Michelle Orane; Artoni,Roberto Ferreira; Moreira Filho,Orlando; Vicari,Marcelo Ricardo.
Parodontidae fish show few morphological characteristics for the identification of their representatives and chromosomal analyses have provided reliable features for determining the interrelationships in this family. In this study, the chromosomes of Apareiodon hasemani from the São Francisco River basin, Brazil, were analyzed and showed a karyotype with 2n = 54 meta/submetacentric chromosomes, and a ZZ/ZW sex chromosome system. The study revealed active NORs located on pair 11 and additional 18S rDNA sites on pairs 7 and 22. The 5S rDNA locus was found in pair 14. It showed a pericentric inversion regarding the ancestral condition. The satellite DNA pPh2004 was absent in the chromosomes of A. hasemani, a shared condition with most members of Apareiodon....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; FISH; 18S rDNA; 5S rDNA; Neotropical fishes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500010

High interpopulation homogeneity in Central Argentina as assessed by Ancestry Informative Markers (AIMs)
Provedor de dados: Genetics and Molecular Biology
Autores: García,Angelina; Dermarchi,Darío A.; Tovo-Rodrigues,Luciana; Pauro,Maia; Callegari-Jacques,Sidia M.; Salzano,Francisco M.; Hutz,Mara H.
The population of Argentina has already been studied with regard to several genetic markers, but much more data are needed for the appropriate definition of its genetic profile. This study aimed at investigating the admixture patterns and genetic structure in Central Argentina, using biparental markers and comparing the results with those previously obtained by us with mitochondrial DNA (mtDNA) in the same samples. A total of 521 healthy unrelated individuals living in 13 villages of the Córdoba and San Luis provinces were tested. The individuals were genotyped for ten autosomal ancestry informative markers (AIMs). Allele frequencies were compared with those of African, European and Native American populations, chosen to represent parental contributions....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AIMs; Córdoba; San Luis; Migration; Population structure.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300324

Atypical chromosome abnormalities in acute myeloid leukemia type M4
Provedor de dados: Genetics and Molecular Biology
Autores: Fett-Conte,Agnes C.; Estrela,Roseli Viscardi; Vendrame-Goloni,Cristina B.; Carvalho-Salles,Andréa B.; Ricci-Júnior,Octávio; Varella-Garcia,Marileila.
This study reports an adult AML-M4 patient with atypical chromosomal aberrations present in all dividing bone marrow cell at diagnosis: t(1;8)(p32.1;q24.2), der(9)t(9;10)(q22;?), and ins(19;9)(p13.3;q22q34) that may have originated transcripts with leukemogenic potential.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myeloid leukemia; Chromosomal abnormalities; Chromosomal translocations.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100002

The Organic Codes: an introduction to semantic biology
Provedor de dados: Genetics and Molecular Biology
Autores: Guimarães,Romeu Cardoso.
Tipo: Info:eu-repo/semantics/article
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100017

Association of S100B polymorphisms and serum S100B with risk of systemic lupus erythematous in a Chinese population
Provedor de dados: Genetics and Molecular Biology
Autores: Lu,Yulan; Huang,Huatuo; Liu,Chunhong; Zeng,Yonglong; Wang,Rong; Wang,Chunfang; Wei,Yesheng; Lan,Yan.
Abstract The aim of this study was to investigate whether the S100B polymorphisms are associated with systemic lupus erythematous (SLE) in a Chinese population. A total of 313 SLE patients and 396 control subjects were enrolled in the present study. The genotypes of three SNPs (rs9722, rs881827 and rs1051169) in S100B gene were detected by single base extension polymerase chain reaction (SBE-PCR). Serum S100B levels were determined by enzyme-linked immunosorbent assay (ELISA). Rs1051169 was associated with an increased risk of SLE (C vs. G: adjusted OR=1.46, 95% CI, 1.18-1.80, p=0.001; CC vs. GG: adjusted OR=1.99, 95% CI, 1.32-3.02, p=0.001; CC+GC vs. GG: adjusted OR=1.54, 95% CI, 1.13-2.11, p=0.007; CC vs. GC+GG: adjusted OR=1.67, 95% CI, 1.16-2.42,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: S100B; Polymorphisms; Serum levels; SLE; Neurologic disorder.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300321

Enzyme replacement therapy for Fabry disease: a systematic review and meta-analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Alegra,Taciane; Vairo,Filippo; de Souza,Monica V.; Krug,Bárbara C.; Schwartz,Ida V.D.
The specific treatment available for Fabry disease (FD) is enzyme replacement therapy (ERT) with agalsidase alfa or beta. A systematic review and meta-analysis was conducted to assess the efficacy and safety of ERT for FD. Only double-blind, randomized clinical trials (RCTs) comparing agalsidase alfa or beta and placebo were included. ERT with either agalsidase alfa or beta was considered similar for the purposes of analysis. Ten RCTs were identified, which showed improvements in neuropathic pain, in heart abnormalities and in globotriaosylceramide (GL-3) levels. A meta-analysis showed increased odds for fever, rigors, development of IgG antibodies to agalsidase, and no significant association with development of hypertension or reduction in the QRS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fabry disease; Fabry disease/therapy; Enzyme replacement therapy; Alpha-Galactosidase.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600009

Genetic counseling and presymptomatic testing programs for Machado-Joseph disease: lessons from Brazil and Portugal
Provedor de dados: Genetics and Molecular Biology
Autores: Schuler-Faccini,Lavínia; Osorio,Claudio Maria; Romariz,Flavia; Paneque,Milena; Sequeiros,Jorge; Jardim,Laura Bannach.
Machado-Joseph disease (MJD) is an autosomal dominant, late-onset neurological disorder and the most common form of spinocerebellar ataxia (SCA) worldwide. Diagnostic genetic testing is available to detect the disease-causing mutation by direct sizing of the CAG repeat tract in the ataxin 3 gene. Presymptomatic testing (PST) can be used to identify persons at risk of developing the disease. Genetic counseling provides patients with information about the disease, genetic risks, PST, and the decision-making process. In this study, we present the protocol used in PST for MJD and the relevant observations from two centers: Brazil (Porto Alegre) and Portugal (Porto). We provide a case report that illustrates the significant ethical and psychological issues...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SCA3; Machado-Joseph; Presymptomatic test; Ataxia; Genetic testing; Psychosocial; Psychological issues.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200012

Allozymic variation and differentiation in the chilean blue mussel, Mytilus chilensis, along its natural distribution
Provedor de dados: Genetics and Molecular Biology
Autores: Toro,Jorge E.; Castro,Grace C.; Ojeda,Johana A.; Vergara,Ana M.
Genetic differentiation in the Chilean blue mussel Mytilus chilensis (Hupé 1854) was investigated based on the variation in the allozyme frequencies of Pgm, Gpi, Icd, Me, Gsr, Lap and Pep in eight samples collected along 1800 km from Arauco (VIII Region) to Punta Arenas (XII Region). Despite the large geographic separations, values of Neis unbiased genetic distance, D (0.004-0.048) and standardised genetic variation among populations, Fst (0.011-0.055) were small. The levels of gene flow (Nm = 8) found in this study prevent the effect of differentiation among populations by genetic drift. This findings indicate that its long-lived planktotrophic larvae provides this species with considerable dispersal ability throughout its range which is favoured by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allozyme; Mytilus chilensis; Population genetics; Gene flow; Chile.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100031

Genetic variability in five species of Anostomidae (Ostariophysi - Characiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Chiari,Lucimara; Sodré,Leda Maria Koelblinger.
Genetic variability was studied in five fish species (Anostomidae): Schizodon intermedius and S. nasutus and Leporinus friderici, L. elongatus and L. obtusidens, collected at one location on the Tibagi River (Paraná, Brazil). The protein data from seven systems coded collectively for 19 loci in the liver, muscle and heart. Nine of these loci were polymorphic. The estimated proportion of polymorphism loci (&lt;img src="http:/img/fbpe/gmb/v22n4/si_p_.gif" align="absmiddle"&gt;) varied from 16.7% in S. intermedius to 36.9% in L. friderici; the mean heterozygosity observed (&lt;img src="http:/img/fbpe/gmb/v22n4/si_h_.gif" alt="si_h_.gif (76 bytes)" align="absmiddle"&gt;o) was 0.027 ± 0.015 and 0.109 ± 0.042, respectively. The estimated value of the genetic...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400009

The phenotype strikes back and is out on more than one limb: a review of Developmental Plasticity and Evolution, by Mary Jane West-Eberhard
Provedor de dados: Genetics and Molecular Biology
Autores: Hartfelder,Klaus.
Tipo: Info:eu-repo/semantics/article
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300025

Antioxidant responses of wheat plants under stress
Provedor de dados: Genetics and Molecular Biology
Autores: Caverzan,Andréia; Casassola,Alice; Brammer,Sandra Patussi.
Abstract Currently, food security depends on the increased production of cereals such as wheat (Triticum aestivum L.), which is an important source of calories and protein for humans. However, cells of the crop have suffered from the accumulation of reactive oxygen species (ROS), which can cause severe oxidative damage to the plants, due to environmental stresses. ROS are toxic molecules found in various subcellular compartments. The equilibrium between the production and detoxification of ROS is sustained by enzymatic and nonenzymatic antioxidants. In the present review, we offer a brief summary of antioxidant defense and hydrogen peroxide (H2O2) signaling in wheat plants. Wheat plants increase antioxidant defense mechanisms under abiotic stresses, such...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic stress; Antioxidant enzymes; Hydrogen peroxide signaling; Wheat; Triticum aestivum.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100001

Enhanced levels of the apoptotic BAX/BCL-2 ratio in children with acute lymphoblastic leukemia and high-risk features
Provedor de dados: Genetics and Molecular Biology
Autores: Kaparou,Maria; Choumerianou,Despoina; Perdikogianni,Chrysoula; Martimianaki,Georgia; Kalmanti,Maria; Stiakaki,Eftichia.
It has been suggested that leukemia is characterized by an impaired balance between the proliferation of blood cells and their capacity to undergo apoptosis. The aim of this study was to examine the expression of key molecules related to apoptosis (BCL-2, BAX, FAS, FAS-L) in children with acute lymphoblastic leukemia (ALL). Measurement of BCL-2 and BAX mRNA was performed by quantitative real-time PCR, and membrane expression of FAS and FAS-L was assessed by flow cytometry in bone marrow mononuclear cells, both at diagnosis and at remission following induction chemotherapy. At diagnosis, increased levels of the apoptotic BAX/BCL-2 ratio were observed in children older than 10 years and with higher white blood cell counts. A DNA index &lt; 1.16 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukemia; Real-time PCR analysis; Protein.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100002

A lethal effect associated with polymorphism of the NOR-bearing chromosomes in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: Genetics and Molecular Biology
Autores: Porto-Foresti,Fábio; Oliveira,Claudio; Gomes,Eduardo A.; Tabata,Yara A.; Rigolino,Marcos G.; Foresti,Fausto.
Cytogenetic analysis of a rainbow trout stock showed that the nucleolar organizing regions were located subterminally on the long arm of a submetacentric chromosome pair and occurred as a single chromosomal segment (phenotype N1) or as two chromosomal segments separated by a short euchromatic segment (phenotype N2). Cytogenetic analysis also showed that there were N1N1 and N1N2 individuals but no N2N2 individuals. Analysis of the different includedphenotypes incluted that the population was not in Hardy-Weinberg equilibrium (chi² = 19.333; p &lt; 0.01), and that a higher frequency of individuals had the N1N2 phenotype. Experimental crosses involving four males (two N1N1 and two N1N2) and four females (one N1N1 and three N1N2) yielded eight broods. There...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NOR polymorphism; Paracentric inversion; Fish cytogenetics; Rainbow trout.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100009

Uniparental genetic markers in South Amerindians
Provedor de dados: Genetics and Molecular Biology
Autores: Bisso-Machado,Rafael; Bortolini,Maria Cátira; Salzano,Francisco Mauro.
A comprehensive review of uniparental systems in South Amerindians was undertaken. Variability in the Y-chromosome haplogroups were assessed in 68 populations and 1,814 individuals whereas that of Y-STR markers was assessed in 29 populations and 590 subjects. Variability in the mitochondrial DNA (mtDNA) haplogroup was examined in 108 populations and 6,697 persons, and sequencing studies used either the complete mtDNA genome or the highly variable segments 1 and 2. The diversity of the markers made it difficult to establish a general picture of Y-chromosome variability in the populations studied. However, haplogroup Q1a3a* was almost always the most prevalent whereas Q1a3* occurred equally in all regions, which suggested its prevalence among the early...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetics; Language and geography; Mitochondrial DNA; Native Americans; South Amerindians; Y-chromosome.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300001

The rs10455872-G allele of the LPA gene is associated with high lipoprotein(a) levels and increased aortic valve calcium in a Mexican adult population
Provedor de dados: Genetics and Molecular Biology
Autores: Cardoso-Saldaña,Guillermo; Fragoso,José Manuel; Lale-Farjat,Shamar; Torres-Tamayo,Margarita; Posadas-Romero,Carlos; Vargas-Alarcón,Gilberto; Posadas-Sánchez,Rosalinda.
Abstract Polymorphisms in the LPA gene have been associated with aortic valve calcification (AVC). There are wide differences in the allelic frequencies, Lp(a) levels, and the association with AVC among ethnic groups. The aim of this study was to determine the association of the LPA gene polymorphisms with Lp(a) levels and risk of developing AVC, in Mexican-Mestizos population. Six LPA polymorphisms (rs10455872, rs7765803, rs6907156, rs1321195, rs12212807 and rs6919346) were genotyped by TaqMan assays in 1,265 individuals without premature coronary artery disease. The presence of AVC was determined by computed tomography. The association of the LPA polymorphisms with AVC, Lp(a), and other cardiovascular risk factors (CVRF) was evaluated using logistic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aortic valve calcification; Genetic susceptibility; LPA gene polymorphism.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400519

Transcriptional regulators and regulatory pathways involved in prostate gland adaptation to a hypoandrogen environment
Provedor de dados: Genetics and Molecular Biology
Autores: Nishan,Umar; da Rosa-Ribeiro,Rafaela; Damas-Souza,Danilo Marchete; Barbosa,Guilherme Oliveira; Carvalho,Hernandes F.
Abstract Anti-androgen therapies, including orchiectomy, are effective at promoting prostate cancer remission, but are followed by progression to the more aggressive castration-resistant prostate cancer (CRPC). Castration promotes gland and tumor shrinkage. However, prostate adaptation to androgen deprivation involves striking parallel events, all requiring changes in gene expression. We hypothesized that transcription factors (TF) and other transcription-related genes are needed to orchestrate those changes. In this work, downstream analysis using bioinformatic tools and published microarray data allowed us to identify sixty transcriptional regulators (including 10 TF) and to integrate their function in physiologically relevant networks. Functional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Androgens; Arnt; Castration; Prostate; Transcription factors.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500802

Three synonymous genes encode calmodulin in a reptile, the Japanese tortoise, Clemmys japonica
Provedor de dados: Genetics and Molecular Biology
Autores: Shimoda,Kouji; Miyake,Toshihiro; Kimura,Jun; Maejima,Kazuyoshi.
Three distinct calmodulin (CaM)-encoding cDNAs were isolated from a reptile, the Japanese tortoise (Clemmys japonica), based on degenerative primer PCR. Because of synonymous codon usages, the deduced amino acid (aa) sequences were exactly the same in all three genes and identical to the aa sequence of vertebrate CaM. The three cDNAs, referred to as CaM-A, -B, and -C, seemed to belong to the same type as CaMI, CaMII, and CaMIII, respectively, based on their sequence identity with those of the mammalian cDNAs and the glutamate codon biases. Northern blot analysis detected CaM-A and -B as bands corresponding to 1.8 kb, with the most abundant levels in the brain and testis, while CaM-C was detected most abundantly in the brain as bands of 1.4 and 2.0 kb. Our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reptile; CDNA; Synonymousgenes.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100009

Description of the karyotypes of Stejneger's beaked whale (Mesoplodon stejnegeri) and Hubbs’ beaked whale (M. carlhubbsi)
Provedor de dados: Genetics and Molecular Biology
Autores: Kurihara,Nozomi; Tajima,Yuko; Yamada,Tadasu K.; Matsuda,Ayaka; Matsuishi,Takashi.
Abstract The genus Mesoplodon (Cetacea: Odontoceti: Ziphiidae) is one of the few cetacean genera with the karyotype 2n = 42. The 2n = 42 karyotype of M. europaeus and M. carlhubbsi is largely consistent with the general cetacean karyotype 2n = 44, although other 2n = 42 karyotypes do not exhibit clear homologies with the general cetacean karyotype. Therefore, the chromosomes of Mesoplodon species may be the key to understanding cetacean karyological evolution. In the present study, the male karyotypes of M. stejnegeri and M. carlhubbsi were examined. In both species, the diploid number of the male karyotype was 42. Both species had the following characteristics: 1) a huge subtelocentric X chromosome with a large C-block; 2) a small metacentric Y...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Chromosome; Mesoplodon stejnegeri; Mesoplodon carlhubbsi.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500803

Polymorphism of exon 2-3 of bovine major histocompatibility complex class I BoLa-A gene
Provedor de dados: Genetics and Molecular Biology
Autores: Bhushan,Bharat; Patra,Biswa Nath; Das,Pranab Jyoti; Dutt,Triveni; Kumar,Pushpendra; Sharma,Arjava; Umang,; Dandapat,Satyabarta; Ahlawat,Shiv Pal Singh.
The exon 2-3 region of bovine major histocompatibility complex (MHC) class I BoLa-A gene was investigated for polymorphisms in three breeds of cattle originated in the Indian subcontinent namely Sahiwal, Tharparkar, Hariana, as well as crossbred (Bos taurus x Bos indicus) cattle and Jersey, the exotic breed (Bos taurus). The PCR amplified fragment of 714 bp showed distinct DdeI-, TaqI- and HinfI- RFLP patterns, thus confirming a higher degree of polymorphism in this region. To our knowledge this is the first report of HinfI restriction patterns for BoLa-A exon 2-3. The sequencing results revealed a number of nucleotide substitutions in this region, which resulted in amino acid changes. The present investigation confirmed that MHC class I BoLa-A exon 2-3 is...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: MHC; BoLa-A polymorphism; Cattle.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400009

Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera)
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Laiana Cristina da; Azeredo-Oliveira,Maria Tercília Vilela de; Tartarotti,Ester.
Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triatoma klugi; Triatomine; Cytogenetics; Nucleolus; Karyotype; Meiosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300008

Neurospora crassa mat A-2 and mat A-3 proteins weakly interact in the yeast two-hybrid system and affect yeast growth
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Carla C. da; Cruz,Rosana C.; Bucciarelli-Rodriguez,Mônica; Vilas-Boas,Adlane.
Mating-type genes control the entry into the sexual cycle, mating identity and sexual development in fungi. The mat A-2 and mat A-3 genes, present in the mat A idiomorph of the filamentous fungus Neurospora crassa, are required for post-fertilization functions but are not essential for mating identity. Their putative roles as transcription factors are based on the similarity of mat A-2 with the Podospora anserina SMR1 gene and an HMG motif present in the mat A-3 gene. In this work the yeast two-hybrid system was used to identify transcriptional activity and protein-protein interaction of N. crassa mat A-2 and mat A-3 genes. We observed that the mat A-3 protein alone is capable of weakly activating transcription of yeast reporter genes; it also binds with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurospora crassa; Mating-type; Yeast two-hybrid system.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200023

Autonomous growth of BALB/MK keratinocytes transfected   with a retroviral vector carrying the human epidermal growth factor gene
Provedor de dados: Genetics and Molecular Biology
Autores: Choudhuri,Jomuna V.; Mathor,Monica B.; Silva,Flávia H.; Han,Sang W.
Epidermal growth factor (EGF), which promotes epidermal regeneration and wound closure, is important for the proliferation and differentiation of epidermal and epithelial tissues in animals. Exogenous EGF is a promising therapeutic agent for wound healing, but its general use is restricted by the limited availability of this protein. In this work, we show that the transfection of mouse BALB/MK keratinocytes, which are totally dependent on EGF for growth and migration, with mature cDNA for human EGF via a retroviral vector abolished the cells requirement for exogenous EGF. The transformed cells had normal morphology and a growth rate that varied according to the source of the retroviral vector used. Keratinocyte transfection with EGF cDNA provides a time-...
Palavras-chave: BALB/MK keratinocytes; Epidermal growth factor; Retroviral vector.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500003

Genetic control of common bean (Phaseolus vulgaris) resistance to powdery mildew (Erysiphe polygoni)
Provedor de dados: Genetics and Molecular Biology
Autores: Rezende,Viviane Ferreira; Ramalho,Magno Antonio Patto; Corte,Hercules Renato.
Genetic control of common bean (Phaseolus vulgaris) resistance to powdery mildew (Erysiphe polygoni) was studied using segregating populations from the bean variety crosses Jalo x ESAL 686 and ESAL 550 x ESAL 686. F2 plants, together with the parents, were inoculated and evaluated using a scale of values from one (plant without symptoms) to nine (completely infected plant). F2 plants were harvested individually, and F2:3 families were obtained. These families were evaluated in an 11 x 11 and 12 x 12 simple lattice statistical design for the Jalo x ESAL 686 and ESAL 550 x ESAL 686 crosses, respectively, using the same value scale as the F2 generation. The segregation observed in F2 plants and F2:3 families indicated that two genes are involved in genetic...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200017

Divergent evolution and purifying selection of the H (FUT1) gene in New World monkeys (Primates, Platyrrhini)
Provedor de dados: Genetics and Molecular Biology
Autores: Borges,Bárbara do Nascimento; Harada,Maria Lúcia.
In the present study, the coding region of the H gene was sequenced and analyzed in fourteen genera of New World primates (Alouatta, Aotus, Ateles, Brachyteles, Cacajao, Callicebus, Callithrix, Cebus, Chiropotes, Lagothrix, Leontopithecus, Pithecia, Saguinus, and Saimiri), in order to investigate the evolution of the gene. The analyses revealed that this coding region contains 1,101 nucleotides, with the exception of Brachyteles, the callitrichines (Callithrix, Leontopithecus, and Saguinus) and one species of Callicebus (moloch), in which one codon was deleted. In the primates studied, the high GC content (63%), the nonrandom distribution of codons and the low evolution rate of the gene (0.513 substitutions/site/MA in the order Primates) suggest the action...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: H gene; Molecular evolution; Purifying selection; Divergent evolution; New World monkeys.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300007

Cytogenetic studies of two freshwater sciaenids of the genus Plagioscion (Perciformes, Sciaenidae) from the central Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Feldberg,Eliana; Porto,Jorge Ivan Rebelo; Santos,Elen Bethlen Pedraça dos; Valentim,Francisco Carlos Souza.
Cytogenetic characterization of two freshwater sciaenid species from the genus Plagioscion (P. squamosissimus and Plagioscion sp.) was obtained for the first time. Giemsa staining, Ag-NOR and C-banding revealed that both species presented 2n = 48 chromosomes (almost all acrocentric). Single NORs and heterochromatin were found mainly at the pericentromeric position. Karyotypic formulae and NOR location proved to be valuable in showing both interspecific and intraspecific differences. All chromosomes were acrocentric in P. squamosissimus. NORs were located at proximal positions on the long arms of the last chromosome pair of the complement, and were heteromorphic due to size differences. Such heteromorphic NORs seem to be associated with each population...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300011

Evaluation of gene expression profiling in a mouse model of L-gulonolactone oxidase gene deficiency
Provedor de dados: Genetics and Molecular Biology
Autores: Yan,Jian; Jiao,Yan; Li,Xinmin; Jiao,Feng; Beamer,Wesley G.; Rosen,Cliff J.; Gu,Weikuan.
Humans and guinea pigs are species which are unable to synthesize ascorbic acid (vitamin C) because, unlike rodents, they lack the enzyme L-gulonolactone oxidase (Gulo). Although the phenotype of lacking vitamin C in humans, named scurvy, has long been well known, information on the impact of lacking Gulo on the gene expression profiles of different tissues is still missing. This knowledge could improve our understanding of molecular pathways in which Gulo may be involved. Recently, we discovered a deletion that includes all 12 exons in the gene for Gulo in the sfx mouse, characterized by spontaneous bone fractures. We report here the initial analysis of the impact of the Gulo gene deletion on the murine gene expression profiles in the liver, femur and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spontaneous fracture; Bone; Gene expression; L-gulonolactone oxidase; Vitamin C.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300004

Nucleolar organizer regions and a new chromosome number for Rhea americana (Aves: Rheiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Gunski,Ricardo José; Giannoni,Miriam Luz.
Sequential banding analysis (Giemsa-C-banding-Ag NOR) of chromosomes of the common rhea (Rhea americana) was performed. Metaphases were obtained by peripheral blood lymphocyte culture and monolayer embryo cell culture. The diploid chromosome number was 80, different from the 2n = 82 in previous reports. Macrochromosome pairs 1, 2 and 5 were submetacentric and pair 3, subacrocentric. The 4th pair was acrocentric and all of the microchromosomes appeared to be acrocentric, with the exception of a clearly metacentric pair which was fully heterochromatic. The Z was slightly larger than the W, both being acrocentric and C-band negative. Nucleolar organizer regions were observed in the secondary constriction of a microchromosome pair. Correct identification of...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200006

Classification, expression pattern and comparative analysis of sugarcane expressed sequences tags (ESTs) encoding glycine-rich proteins (GRPs)
Provedor de dados: Genetics and Molecular Biology
Autores: Fusaro,Adriana; Mangeon,Amanda; Junqueira,Ricardo Magrani; Rocha,Carla Andréa Benício; Coutinho,Tatiana Cardoso; Margis,Rogério; Sachetto-Martins,Gilberto.
Since the isolation of the first glycine-rich proteins (GRPs) in plants a wealth of new GRPs have been identified. The highly specific but diverse expression pattern of grp genes, taken together with the distinct sub-cellular localization of some GRP groups, clearly indicate that these proteins are involved in several independent physiological processes. Notwithstanding the absence of a clear definition of the role of GRPs in plant cells, studies conducted with these proteins have provided new and interesting insights into the molecular biology and cell biology of plants. Complexly regulated promoters and distinct mechanisms for the regulation of gene expression have been demonstrated and new protein targeting pathways, as well as the exportation of GRPs...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100035

A program for representing and simulating population genetic phenomena
Provedor de dados: Genetics and Molecular Biology
Autores: Nuin,Paulo A.S.; Otto,P.A.
The paper describes a computer program for representing and simulating population genetic phenomena, such as the distribution of gene and genotype frequencies under different mating systems (panmixia, inbreeding and assortative mating systems) and under influence of evolution factors (mutation, selection, gene flow and genetic drift). The program was written in Visual Basic (Microsoft, Inc.) and is able to run in any IBM-PC compatible computer running Windows 3.1 or later versions.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100009

The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Mäder,Geraldo; Zamberlan,Priscilla M.; Fagundes,Nelson J.R.; Magnus,Tielli; Salzano,Francisco M.; Bonatto,Sandro L.; Freitas,Loreta B.
The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Intraspecific variability; ITS; Passiflora; Phylogeography.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018

Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Ana Maria Pujol Vieira dos; Matsumura,Aida T. Santos; Van Der Sand,Sueli Teresinha.
The phytopathogenic fungus Drechslera tritici-repentis causes tan spot, an important disease of wheat in the southern Brazilian state of Rio Grande do Sul. Twelve D. tritici-repentis isolates were obtained from wheat seeds from different locations in the state. Their colony morphology on potato dextrose agar and polymorphisms in genomic DNA by the random amplified polymorphic DNA (RAPD) method were investigated. For the RAPD method, 23 primers were tested of which nine were selected for use in the study of D. tritici-repentis polymorphisms. The degree of similarity between isolates was calculated using a simple matching coefficient and dendrograms constructed by the unweighted pair-group method with arithmetical averages (UPGMA). The morphological and RAPD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drechslera tritici-repentis; RAPD; DNA; Polymorphism; Genetic diversity.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200020

Genetic polymorphism among 14 elite Coffea arabica L. cultivars using RAPD markers associated with restriction digestion
Provedor de dados: Genetics and Molecular Biology
Autores: Sera,Tumoru; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Diniz,Leandro Eugênio Cardamone; Carvalho,Valdemar de Paula; Rampim,Leandro; Ruas,Eduardo Augusto; Silveira,Sheila Recepute da.
Knowledge of the genetic variability among genotypes is important for the transfer of useful genes and to maximize the use of available germplasm resources. This study was carried out to assess the genetic variability of 14 elite Coffea arabica cultivars using random amplified polymorphic DNA (RAPD) associated with a prior digestion of genomic DNA with restriction endonucleases. The accessions were obtained from the Coffea collection maintained at the Instituto Agronômico do Paraná (IAPAR), located in Londrina, Paraná, Brazil. Twenty-four informative RAPD primers, used in association with restriction enzymes, yielded 330 reproducible and scorable DNA bands, of which 224 (68%) were polymorphic. The amplified products were used to estimate the genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica; RAPD; Restriction endonucleases.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100010

Seeing the FORESTs for the trees
Provedor de dados: Genetics and Molecular Biology
Autores: Carrer,Helaine.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400001

Regulation of adipogenesis by nucelar receptor PPARγ is modulated by the histone demethylase JMJD2C
Provedor de dados: Genetics and Molecular Biology
Autores: Fernando,Lizcano; Carolina,Romero; Vargas,Diana.
A potential strategy to combat obesity and its associated complications involves modifying gene expression in adipose cells to reduce lipid accumulation. The nuclear receptor Peroxisome Proliferator-activated receptor gamma (PPARγ) is the master regulator of adipose cell differentiation and its functional activation is currently used as a therapeutic approach for Diabetes Mellitus type 2. However, total activation of PPARγ induces undesirable secondary effects that might be set with a partial activation. A group of proteins that produce histone demethylation has been shown to modify the transcriptional activity of nuclear receptors. Here we describe the repressive action of the jumonji domain containing 2C/lysine demethylase 4 C (JMJD2C/KDM4C) on PPARγ...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PPARγ; Histones; Demethylation; JMJD2C; Deacetylation; Adipocyte; Trichostatin A.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100004

Functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum
Provedor de dados: Genetics and Molecular Biology
Autores: Kajihara,Daniela; Godoy,Fabiana de; Hamaji,Thais Alves; Blanco,Silvia Regina; Van Sluys,Marie-Anne; Rossi,Magdalena.
Transposable elements (TEs) account for a large portion of plant genomes, particularly in grasses, in which they correspond to 50%-80% of the genomic content. TEs have recently been shown to be a source of new genes and new regulatory networks. The most striking contribution of TEs is referred as “molecular domestication”, by which the element coding sequence loses its movement capacity and acquires cellular function. Recently, domesticated transposases known as mustang and derived from the Mutator element have been described in sugarcane. In order to improve our understanding of the function of these proteins, we identified mustang genes from Sorghum bicolor and Zea mays and performed a phenetic analysis to assess the diversity and evolutionary history of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Domesticated tranposases; Saccharum sp.; Sorghum bicolor; Transposable elements; Zea mays.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400014

Cora de Moura Pedreira (1915-2005)
Provedor de dados: Genetics and Molecular Biology
Autores: Moreira,Lilia Maria de Azevedo.
Tipo: Info:eu-repo/semantics/other
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300002

Morphological divergence rate tests for natural selection: uncertainty of parameter estimation and robustness of results
Provedor de dados: Genetics and Molecular Biology
Autores: Monteiro,Leandro R.; Gomes-Jr,José L.
In this study, we used a combination of geometric morphometric and evolutionary genetics methods for the inference of possible mechanisms of evolutionary divergence. A sensitivity analysis for the constant-heritability rate test results regarding variation in genetic and demographic parameters was performed, in order to assess the relative influence of uncertainty of parameter estimation on the robustness of test results. As an application, we present a study on body shape variation among populations of the poeciliine fish Poecilia vivipara inhabiting lagoons of the quaternary plains in northern Rio de Janeiro State, Brazil. The sensitivity analysis showed that, in general, the most important parameters are heritability, effective population size and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Divergence rate test; Natural selection; Poecilia vivipara; Statistical shape analysis; Geographic variation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200028

Identification of candidate genes for lung cancer somatic mutation test kits
Provedor de dados: Genetics and Molecular Biology
Autores: Chen,Yong; Shi,Jian-Xin; Pan,Xu-Feng; Feng,Jian; Zhao,Heng.
Over the past three decades, mortality from lung cancer has sharply and continuously increased in China, ascending to the first cause of death among all types of cancer. The ability to identify the actual sequence of gene mutations may help doctors determine which mutations lead to precancerous lesions and which produce invasive carcinomas, especially using next-generation sequencing (NGS) technology. In this study, we analyzed the latest lung cancer data in the COSMIC database, in order to find genomic "hotspots" that are frequently mutated in human lung cancer genomes. The results revealed that the most frequently mutated lung cancer genes are EGFR, KRAS and TP53.In recent years, EGFR and KRAS lung cancer test kits have been utilized for detecting lung...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung cancer; Next-generation sequencing; Somatic mutation kit; COSMIC.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300022

Comparative cytogenetics in three Melipona species (Hymenoptera: Apidae) with two divergent heterochromatic patterns
Provedor de dados: Genetics and Molecular Biology
Autores: Cunha,Marina Souza da; Travenzoli,Natália Martins; Ferreira,Riudo de Paiva; Cassinela,Edson Kuatelela; Silva,Henrique Barbosa da; Oliveira,Francisco Plácido Magalhães; Salomão,Tânia Maria Fernandes; Lopes,Denilce Meneses.
Abstract The genus Melipona is subdivided into four subgenera based on morphological characteristics, and two groups based on cytogenetic patterns. The cytogenetic information on this genus is still scarce, therefore, the goal of this study was to characterize Melipona paraensis, Melipona puncticollis, and Melipona seminigra pernigra using the following techniques: C-banding, DAPI/CMA3 fluorochromes, and FISH with an 18S rDNA probe. Melipona paraensis (2n=18) and M. seminigra pernigra (2n=22) were classified as high heterochromatin content species (Group II). Their euchromatin is restricted to the ends of the chromosomes and is CMA3+; the 18S rDNA probe marked chromosome pair number 4. Melipona puncticollis (2n=18) is a low heterochromatin content species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal evolution; DAPI/CMA3 fluorochromes; Fluorescent in situ Hybridization (FISH); Heterochromatin; Meliponini.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500806

Mitochondrial genome nucleotide substitution pattern between domesticated silkmoth, Bombyx mori, and its wild ancestors, Chinese Bombyx mandarina and Japanese Bombyx mandarina
Provedor de dados: Genetics and Molecular Biology
Autores: Li,Yu-Ping; Song,Wu; Shi,Sheng-Lin; Liu,Yan-Qun; Pan,Min-Hui; Dai,Fang-Yin; Lu,Cheng; Xiang,Zhong-Huai.
Bombyx mori and Bombyx mandarina are morphologically and physiologically similar. In this study, we compared the nucleotide variations in the complete mitochondrial (mt) genomes between the domesticated silkmoth, B. mori, and its wild ancestors, Chinese B. mandarina (ChBm) and Japanese B. mandarina (JaBm). The sequence divergence and transition mutation ratio between B. mori and ChBm are significantly smaller than those observed between B. mori and JaBm. The preference of transition by DNA strands between B. mori and ChBm is consistent with that between B. mori and JaBm, however, the regional variation in nucleotide substitution rate shows a different feature. These results suggest that the ChBm mt genome is not undergoing the same evolutionary process as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nucleotide substitution pattern; Mitochondrial genome; Bombyx mori; Chinese Bombyx mandarina; Japanese Bombyx mandarina.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100031

Esterase profile in a pyrethroid-resistant Brazilian strain of the cattle tick Boophilus microplus (Acari, Ixodidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Baffi,Milla Alves; Pereira,Cícero Donizete; Souza,Guilherme Rocha Lino de; Bonetti,Ana Maria; Ceron,Carlos Roberto; Gourlart,Luiz Ricardo.
The cattle tick Boophilus microplus causes great damage in livestock and is considered one of the most important tropical ectoparasites. The traditional method of control is based on the intensive use of pesticides, however the indiscriminate use of these compounds over the years has led to the selection of resistant ticks. Hydrolases, particularly esterases (EST), have been reported to be associated with acaricide resistance in B. microplus. We compared the esterase profile of susceptible and cypermethrin-resistant strains of adult B. microplus and a pyrethroid susceptible reference strain (the Mozzo strain) using polyacrylamide gel electrophoresis and specific staining. The electrophoretic profiles of protein extracts revealed the presence of four...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Boophilus microplus; Pesticides; Resistance; Esterases.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500016

DNM2 mutations in a cohort of sporadic patients with centronuclear myopathy
Provedor de dados: Genetics and Molecular Biology
Autores: Abath Neto,Osorio; Martins,Cristiane de Araújo; Carvalho,Mary; Chadi,Gerson; Seitz,Katia Werneck; Oliveira,Acary Souza Bulle; Reed,Umbertina Conti; Laporte,Jocelyn; Zanoteli,Edmar.
Centronuclear myopathy (CNM) is a rare congenital muscle disease characterized by fibers with prominent centralized nuclei in muscle biopsies. The disease is clinically heterogeneous, ranging from severe neonatal hypotonic phenotypes to adult-onset mild muscle weakness, and can have multiple modes of inheritance in association with various genes, including MTM1, DNM2, BIN1 and RYR1. Here we analyzed 18 sporadic patients with clinical and histological diagnosis of CNM and sequenced the DNM2 gene, which codes for the dynamin 2 protein. We found DNM2 missense mutations in two patients, both in exon 8, one known (p.E368K) and one novel (p.F372C), which is found in a position of presumed pathogenicity and appeared de novo. The patients had similar phenotypes...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Centronuclear myopathy; DNM2; Dynamin 2; Congenital myopathy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200147

Intrapopulational genetic differentiation in Anopheles (N.) darlingi Root, 1926 (Diptera: Culicidae) in the amazon region
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Joselita Maria Mendes dos; Lobo,Jorge Arturo; Tadei,Wanderli Pedro; Contel,Eucleia P. Betioli.
Four natural Amazonian Anopheles darlingi populations were analyzed for 19 loci, 12 of which showed variation, i.e., 68.4% of polymorphic loci. The Ariquemes, Rondônia, population was the most variable, with a large number of alleles per locus (2.26 ± 0.27) and high polymorphism (P = 63.15). The highest values of observed and expected intralocus heterozygosity were observed in the Manaus, Amazonas, population (Ho = 0.432 ± 0.11; He = 0.375 ± 0.08), and the lowest in the Cachoeira Porteira, Pará, population (Ho = 0.236 ± 0.09; He = 0.290 ± 0.11). Wright's F-statistic revealed disequilibrium caused by an excess of homozygotes, as shown by the Fis &amp;gt; Fst values (Fis = 0.083 &amp;gt; 0.026) reflecting intrapopulational differentiation. The four...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300007

Birth weight of twins: 1. The fetal growth patterns of twins and singletons
Provedor de dados: Genetics and Molecular Biology
Autores: Beiguelman,Bernardo; Colletto,Gloria M.D.D.; Franchi-Pinto,Carla; Krieger,Henrique.
Distributions of birth weights of twins and singletons born at three southeastern Brazilian hospitals were compared after adjustment for gestational age, its quadratic and cubic terms, sex, type of pregnancy, as well as their interactions. The pattern of twin fetal growth rate was retarded in comparison to that of singletons, regardless of the socioeconomic level of the examined population, but the gestational age at which this retardation started seems to be correlated to the income of the mothers. In all cases, after 28 weeks of gestation, female fetal growth was slightly but consistently lower than that of males.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100025

Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisiae diploid strain
Provedor de dados: Genetics and Molecular Biology
Autores: Morais Junior,Marcos Antonio de; Santos,José Ferreira dos; Henriques,João Antonio Pegas.
The Escherichia coli RecA protein (RecAp) has been demonstrated to induce mutagenesis in yeast cells, although there is still little information on the role of the RecAp in yeast recombination events. We evaluated spontaneous and induced general recombination in vegetative and meiotic cells of the XS2316 strain of the yeast Saccharomyces cerevisiae bearing the recA gene. We found that RecAp decreased reciprocal recombination, gene conversion and intrachromosomal recombination and promoted an increase in error-prone processes in both vegetative and meiotic cells, while its negative effect on meiotic recombination blocked ascospore formation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA repair; Genetic recombination; RecA gene; Sporulation; Yeast.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200016

Screening for organic acidurias and aminoacidopathies in high-risk Brazilian patients: Eleven-year experience of a reference center
Provedor de dados: Genetics and Molecular Biology
Autores: Wajner,Moacir; Sitta,Angela; Kayser,Aline; Deon,Marion; Groehs,Ana C.; Coelho,Daniella M.; Vargas,Carmen R.
Abstract Organic acidurias and aminoacidopathies are groups of frequent inborn errors of metabolism (IEMs), which are caused by mutations in specific genes that lead to loss of protein/enzyme or transport function with important deleterious effects to cell metabolism. Since a considerable number of such disorders are potentially treatable when diagnosed at an early stage of life, diagnosis is crucial for the patients. In the present report, we describe symptomatic individuals referred to our service that were diagnosed with these disorders from 2006 to 2016. We used blood and urine samples from 21,800 patients suspected of aminoacidopathies or organic acidemias that were processed by the analytical techniques reverse phase high-performance liquid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organic acidurias; Aminoacidopathies; Inborn errors of metabolism; Selective screening.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200178

The histone genes cluster in Rhynchosciara americana and its transcription profile in salivary glands during larval development
Provedor de dados: Genetics and Molecular Biology
Autores: Siviero,Fábio; Rezende-Teixeira,Paula; Andrade,Alexandre de; Santelli,Roberto Vicente; Machado-Santelli,Glaucia Maria.
Abstract In this work we report the characterization of the Rhynchosciara americana histone genes cluster nucleotide sequence. It spans 5,131 bp and contains the four core histones and the linker histone H1. Putative control elements were detected. We also determined the copy number of the tandem repeat unit through quantitative PCR, as well as the unequivocal chromosome location of this unique locus in chromosome A band 13. The data were compared with histone clusters from the genus Drosophila, which are the closest known homologues.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhynchosciara; Histone cluster; Sciaridae; Codon usage.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400580

Genetic relationships among American species of Prosopis (Leguminosae) based on enzyme markers
Provedor de dados: Genetics and Molecular Biology
Autores: Bessega,Cecilia; Saidman,Beatriz Ofelia; Vilardi,Juan César.
In the present work, isoenzyme electrophoresis was used to analyze the variability and phenetic relationships among seven American species of genus Prosopis belonging to three different sections: P. argentina (Monilicarpa), P. glandulosa, P. velutina, P. flexuosa, P. ruscifolia, P. kuntzei (Algarobia), and P. reptans (Strombocarpa). The genetic variability in P. argentina, P. reptans, and P. kuntzei was significantly lower than in the rest of the species analyzed. The species belonging to different sections are highly differentiated, but the relationships retrieved among species belonging to the section Algarobia suggested that the series of this section are not natural groups. P. kuntzei is as differentiated from the remaining species of Algarobia as from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prosopis; Isoenzymes; Genetic variability; Phenetic relationships.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200018

Cytogenetic characterization of Melipona rufiventris Lepeletier 1836 and Melipona mondury Smith 1863 (Hymenoptera, Apidae) by C banding and fluorochromes staining
Provedor de dados: Genetics and Molecular Biology
Autores: Lopes,Denilce Meneses; Pompolo,Sílvia das Graças; Campos,Lúcio Antônio de Oliveira; Tavares,Mara Garcia.
The stingless bees Melipona rufiventris and M. mondury were analyzed cytogenetically by conventional staining with Giemsa, C-banding and sequential staining with the fluorochromes CMA3/DA/DAPI. Both species presented 2n = 18 and n = 9, except for one colony of M. rufiventris, in which some individuals had 2n = 19 due to the presence of a B chromosome. After Giemsa staining and C-banding the chromosomes appeared very condensed and presented a high heterochromatic content, making it difficult to localize the centromere and therefore to visualize the chromosomes morphology. The constitutive heterochromatin was located in interstitial chromosome regions covering most of the chromosomes extension and consisted mainly of AT, as shown by DAPI staining. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melipona; Stingless bees; B chromosomes; Fluorochromes; C-banding.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100010

Karyological study of cicindelidia trifasciata (coleoptera, cicindelidae) from cuba: evidence of B chromosomes
Provedor de dados: Genetics and Molecular Biology
Autores: Proença,Sónia J.R.; Collares-Pereira,M.J.; Serrano,A.R.M.
Metaphases from germinal tissue of eight males and seven females of Cicindelidia trifasciata from Cuba were analyzed. The species karyotype does not fit the pattern described for Nearctic cicindelids (2n = 18 + XXY): it has 11 autosome pairs and a sex chromosome system of the X1X2X3Y/ X1X1X2X2 X3X3 type, thus a diploid value of 2n = 26 in males and 2n = 28 in females. The first two autosome pairs were almost twice as large as the remaining chromosomes, and the sex elements were morphologically well differentiated. This population was found to be polymorphic for supernumerary chromosomes at both the intraindividual and interindividual levels. An extra small B chromosome was observed in all metaphases of one male (2n = 27), and every female showed metaphases...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100010

RAPD analysis of Nectomys squamipes (Rodentia, Sigmodontinae) populations
Provedor de dados: Genetics and Molecular Biology
Autores: Almeida,Francisca C.; Moreira,Miguel A.M.; Bonvicino,Cibele R.; Cerqueira,Rui.
Random amplified of polymorphic DNA (RAPD) analysis was used to assess genetic distance and the genetic structure of populations of Nectomys squamipes, a semiaquatic rodent species distributed along watercourses. DNA samples of five populations were analyzed using three primers, producing 45 scorable bands, 31 of which were polymorphic. There was a significant differentiation among populations [F ST = 0.17; phiST = 0.14 (P &lt; 0.004)] but gene flow (Nm = 1.25) was sufficient to overcome genetic drift effects. No fixed specific markers were found for any population. The Mantel's test and UPGMA cluster analysis showed a lack of relationship between genetic and geographic distances. The apparent homogeneity indicated by RAPD markers coincided with...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400014

Relationship of an hRAD54 gene polymorphism (2290 C/T) in an Ecuadorian population with chronic myelogenous leukemia
Provedor de dados: Genetics and Molecular Biology
Autores: Paz-y-Miño,César; López-Cortés,Andrés; Muñoz,María José; Castro,Bernardo; Cabrera,Alejandro; Sánchez,María Eugenia.
The hRAD54 gene is a key member of the RAD52 epistasis group involved in repair of double-strand breaks (DSB) by homologous recombination (HR). Thus, alterations of the normal function of these genes could generate genetic instability, shifting the normal process of the cell cycle, leading the cells to develop into cancer. In this work we analyzed exon 18 of the hRAD54 gene, which has been previously reported by our group to carry a silent polymorphism, 2290 C/T (Ala730Ala), associated to meningiomas. We performed a PCR-SSCP method to detect the polymorphism in 239 samples including leukemia and normal control population. The results revealed that the 2290 C/T polymorphism has frequencies of 0.1 for the leukemia and 0.1 for the control group. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Leukemia; CML; ALL; HRAD54; 2290 C/T polymorphism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400009

On the reliability of a simple method for scoring phenotypes to estimate heritability: A case study with pupal color in  Heliconius erato phyllis ,   Fabricius 1775 (Lepidoptera, Nymphalidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Ferreira,Adriano Andrejew; Kucharski,Luiz Carlos; Araújo,Aldo Mellender de.
In this paper, two methods for assessing the degree of melanization of pupal exuviae from the butterfly  Heliconius erato phyllis , Fabricius 1775 (Lepidoptera, Nymphalidae, Heliconiini) are compared. In the first method, which was qualitative, the exuviae were classified by scoring the degree of melanization, whereas in the second method, which was quantitative, the exuviae were classified by optical density followed by analysis with appropriate software. The heritability (h 2  ) of the degree of melanization was estimated by regression and analysis of variance. The estimates of h  2  were similar with both methods, indicating that the qualitative method could be particularly suitable for field work. The low estimates obtained for heritability may have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allocation-priority hypothesis; Butterfly; Optical density; Pupal melanization; Qualitative and quantitative methods.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100028

β-globin haplotypes in normal and hemoglobinopathic individuals from Reconcavo Baiano, State of Bahia, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Wellington dos Santos; Klautau-Guimarães,Maria de Nazaré; Grisolia,Cesar Koppe.
Five restriction site polymorphisms in the β-globin gene cluster (HincII-5'ε, HindIII-Gγ, HindIII-ªγ, HincII-'ψβ1 and HincII-3''ψβ1) were analyzed in three populations (n = 114) from Reconcavo Baiano, State of Bahia, Brazil. The groups included two urban populations from the towns of Cachoeira and Maragojipe and one rural Afro-descendant population, known as the "quilombo community", from Cachoeira municipality. The number of haplotypes found in the populations ranged from 10 to 13, which indicated higher diversity than in the parental populations. The haplotypes 2 (+----),3(----+),4(-+--+)and6(-++-+)onthe βA chromosomes were the most common, and two haplotypes, 9 (-++++)and 14 (++--+), were found exclusively in the Maragojipe population. The other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Afro-descendant; Β-globin; Haplotypes; Hemoglobinopathies.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300002

Chromosomal location of 18S and 5S rDNA sites in Triportheus fish species (Characiformes, Characidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz,Débora; Laudicina,Alejandro; Bertollo,Luiz Antonio Carlos.
The location of 18S and 5S rDNA sites was determined in eight species and populations of the fish genus Triportheus by using fluorescent in situ hybridization (FISH). The males and females of all species had 2n = 52 chromosomes and a ZZ/ZW sex chromosome system. A single 18S rDNA site that was roughly equivalent to an Ag-NOR was detected on the short arms of a submetacentric pair in nearly all species, and up to two additional sites were also observed in some species. In addition, another 18S rDNA cluster was identified in a distal region on the long arms of the W chromosome; this finding corroborated previous evidence that this cluster would be a shared feature amongst Triportheus species. In T. angulatus, a heterozygotic paracentric inversion involving...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal markers; Fish cytogenetics; NOR; Ribosomal genes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100006

Translational control in plant antiviral immunity
Provedor de dados: Genetics and Molecular Biology
Autores: Machado,João Paulo B.; Calil,Iara P.; Santos,Anésia A.; Fontes,Elizabeth P.B.
Abstract Due to the limited coding capacity of viral genomes, plant viruses depend extensively on the host cell machinery to support the viral life cycle and, thereby, interact with a large number of host proteins during infection. Within this context, as plant viruses do not harbor translation-required components, they have developed several strategies to subvert the host protein synthesis machinery to produce rapidly and efficiently the viral proteins. As a countermeasure against infection, plants have evolved defense mechanisms that impair viral infections. Among them, the host-mediated translational suppression has been characterized as an efficient mean to restrict infection. To specifically suppress translation of viral mRNAs, plants can deploy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Translation suppression; Recessive resistance genes; Argonaute; NSP-Interacting Kinase; NIK.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200292

Reprogenetics: preimplantational genetics diagnosis
Provedor de dados: Genetics and Molecular Biology
Autores: Coco,Roberto.
Preimplantational Genetics Diagnosis (PGD) is requested by geneticists and reproductive specialists. Usually geneticists ask for PGD because one or both members of the couple have an increased genetic risk for having an affected offspring. On the other hand, reproductive specialists ask for embryo aneuploidy screening (PGS) to assures an euploid embryo transfer, with the purpose to achieve an ongoing pregnancy, although the couple have normal karyotypes. As embryonic aneuploidies are responsible for pre and post implantation abortions, it is logical to considerer that the screening of the embryonic aneuploidies prior to embryo transfer could improve the efficiency of the in vitro fertilization procedures. Nevertheless, it is still premature to affirm this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PGD; PGS; PGSS; Embryo biopsy; Trophectoderm biopsy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200013

Genetic analysis reveals candidate species in the Scinax catharinae clade (Amphibia: Anura) from Central Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Nogueira,Lídia; Solé,Mirco; Siqueira,Sérgio; Affonso,Paulo Roberto Antunes de Mello; Strüssmann,Christine; Sampaio,Iracilda.
Abstract Scinax (Anura: Hylidae) is a species-rich genus of amphibians (113 spp.), divided into five species groups by morphological features. Cladistic analyses however revealed only two monophyletic clades in these groups: Scinax catharinae and Scinax ruber. Most species from the S. catharinae clade are found in Atlantic rainforest, except for Scinax canastrensis,S. centralis, S. luizotavioi, S. machadoi,S. pombali and S. skaios. In the present work, specimens of Scinax collected in Chapada dos Guimarães, central Brazil, were morphologically compatible with species from theS. catharinae group. On the other hand, genetic analysis based on mitochondrial (16S and 12S) and nuclear (rhodopsin) sequences revealed a nucleotide divergence of 6 to 20% between...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Amphibians; DNA barcode; Cerrado; RDNA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100049

Inherited hemoglobin disorders in an Afro-Amazonian community: Saracura
Provedor de dados: Genetics and Molecular Biology
Autores: Cardoso,Greice Lemos; Takanashi,Silvania Yukiko Lins; Guerreiro,João Farias.
The most common hemoglobinopathies, viz, hemoglobins S and C, and α-and β-thalassemias, were investigated through the molecular screening of 116 subjects from the community of Saracura, comprising fugitive African slaves from farms of the municipality of Santarém, in the west of Pará State, Brazilian Amazon. The observed frequency of the HBB*S gene (0.9%) was significantly lower than that encountered in other Afro-derived communities in the region. Concomitantly, the absence of the HBB*C allele has been reported for most of the Afro-Amazonian communities thus far studied. As remnant populations of quilombos are generally small, the heterogeneous distribution of HBB*S and HBB*C alleles among them is probably due to genetic drift and/or founder effect. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sickle cell disease; Thalassemias; Afro-Amazonian.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400002

Obituary: Joyce Anderson Duffles Andrade (1938-2009)
Provedor de dados: Genetics and Molecular Biology
Autores: Smith,Marília de Arruda Cardoso.
Tipo: Info:eu-repo/semantics/report
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400001

Development and characterization of 20 polymorphic microsatellite markers for Epinephelus marginatus (Lowe, 1834) (Perciformes: Epinephelidae) using 454 pyrosequencing
Provedor de dados: Genetics and Molecular Biology
Autores: Vaini,Jussara Oliveira; Mota,Kenneth Gabriel; Ojeda,Alejandra Paola; Barreiros,João Pedro; Moreira,Renata Guimarães; Hilsdorf,Alexandre Wagner Silva.
Abstract The dusky grouper, Epinephelus marginatus, is a well-known and widespread marine fish assessed as endangered by the International Union for the Conservation of Nature. Analyzing the genetic diversity of this species is, therefore, of utmost importance and necessary for conservation purposes. Microsatellites are molecular tools with advantages that are ideal for population analyses. This study provides the first set of species-specific microsatellite loci for E. marginatus that can be applied when assessing both intra- and interpopulation genetic variation. Twenty microsatellite loci were isolated and characterized for the dusky grouper by genotyping 20 individuals obtained from the North Eastern Atlantic Ocean (n = 4) and from the South Western...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Short tandem repeat; Population genetics; Conservation; Fishery; Marine resources.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100074

Comparison between the micronucleus frequencies of kidney and gill erythrocytes in tilapia fish, following mitomycin C treatment
Provedor de dados: Genetics and Molecular Biology
Autores: Palhares,Dario; Grisolia,Cesar Koppe.
The frequencies of mitomycin C and cyclophosphamide-induced kidney and gill erythrocyte micronuclei were compared in Tilapia rendalli and Oreochromis niloticus. Cyclophosphamide (CP) and mitomycin C (MMC) were previously used to investigate fish sensitivity to micronuclei induction in kidney erythrocytes. Fish were treated with a single dose of cyclophosphamide (40 mg/kg) or mitomycin C (2 mg/kg). Peripheral blood samples and kidney blood samples were obtained, 1, 2, 3, 5, 7, and 15 days after the intra-abdominal injection of MMC. T. rendalli showed to be more sensitive than O. niloticus to micronuclei production. Both compounds were used at the maximum tolerated dose (MTD), and mitomycin C proved to be more toxic than cyclophosphamide. The frequency of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish micronuclei; Cyclophosphamide; Mitomycin C; Tilapia rendalli; Oreochromis niloticus.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300005

Animal models for human contiguous gene syndromes and other genomic disorders
Provedor de dados: Genetics and Molecular Biology
Autores: Walz,Katherina; Fonseca,Patricia; Lupski,James R.
Genomic disorders refer to a group of syndromes caused by DNA rearrangements, such as deletions and duplications, which result in an alteration of normal gene dosage. The chromosomal rearrangements are usually relatively small and often difficult to detect cytogenetically. In a subset of such conditions the rearrangements comprise multiple unrelated contiguous genes that are physically linked and thus have been referred to as contiguous gene syndromes (CGS). In general, each syndrome presents a complex clinical phenotype that has been attributed generally to dosage sensitive gene(s) present in the responsible chromosomal interval. A common mechanism for CGS resulting from interstitial deletion/duplication has recently been elucidated. The DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genomic disorders; Contiguous gene syndromes; Microdeletion; Microduplication.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300001

Generation mean analysis for quantitative traits in sesame (Sesamum indicum L.) crosses
Provedor de dados: Genetics and Molecular Biology
Autores: Sharmila,Vijayarajan; Ganesh,Siruthaiyur Krishnamoorthy; Gunasekaran,Mahalingam.
To study the nature and magnitude of gene effects for yield and its components in sesame (Sesamum indicum L.) we carried out generation mean analysis using the following four crosses of different sesame cultivars: VS 9510 x Co1; NIC 7907 x TMV 3; Cianno 13/10x VRI 1; and Si 1115/1 x TMV 3. The P1, P2, F1, F2, BC1 and BC2 of these generations were studied for seven quantitative traits. The analysis showed the presence of additive, dominance and epistatic gene interactions. The additive dominance model was adequate for plant height in the NIC 7907 x TMV3 and Si 1115/1x TMV 3 crosses and for capsule length in the VS 9510 x Co1, NIC 7907 x TMV 3 and Si 1115/1 x TMV 3 crosses. An epistatic digenic model was assumed for the remaining crosses. Duplicate-type...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sesame; Quantitative trait; Scaling tests; Gene effects; Bi-parental mating.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100015

Genetic similarity among soybean (Glycine max (L) Merrill) cultivars released in Brazil using AFLP markers
Provedor de dados: Genetics and Molecular Biology
Autores: Bonato,Ana Lídia V.; Calvo,Eberson S.; Geraldi,Isaias O.; Arias,Carlos Alberto A.
Genetic similarity among soybean genotypes was studied by applying the amplified fragment length polymorphism (AFLP) technique to 317 soybean cultivars released in Brazil from 1962 to 1998. Genetic similarity (GS) coefficients were estimated using the coefficient of Nei and Li (Nei and Li 1979), and the cultivars were clustered using the unweighted pair-group method with averages (UPGMA). The parentage coefficients of 100 cultivars released between 1984 and 1998 were calculated and correlated with the genetic similarity obtained by the markers. The genetic similarity coefficients varied from 0.17 to 0.97 (x = 0.61), with 56.8% of the coefficients being above 0.60 and only 9.7% equal to or less than 0.50. The similarity coefficients have remained constant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Genetic similarity; Molecular markers; Parentage coefficient; Soybean.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400019

Conversion of barley SNPs into PCR-based markers using dCAPS method
Provedor de dados: Genetics and Molecular Biology
Autores: Shahinnia,Fahimeh; Sayed-Tabatabaei,Badraldin Ebrahim.
Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction endonuclease for its recognition site and can be used to virtually detect any SNP. Here, we describe the use of the dCAPS method for detecting single-nucleotide changes by means of a barley EST, CK569932, PCR-based marker.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Barley (Hordeum vulgare L.); Genome sequencing; Mismatched primer; Restriction enzyme; SNP genotyping.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300020

Phylogenetic incongruence inferred with two mitochondrial genes in Mepraia spp. and Triatoma eratyrusiformis (Hemiptera, Reduviidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Campos-Soto,Ricardo; Torres-Pérez,Fernando; Solari,Aldo.
Mitochondrial DNA (mtDNA) is widely used to clarify phylogenetic relationships among and within species, and to determine population structure. Due to the linked nature of mtDNA genes it is expected that different genes will show similar results. Phylogenetic incongruence using mtDNA genes may result from processes such as heteroplasmy, nuclear integration of mitochondrial genes, polymerase errors, contamination, and recombination. In this study we used sequences from two mitochondrial genes (cytochrome b and cytochrome oxidase subunit I) from the wild vectors of Chagas disease, Triatoma eratyrusiformis and Mepraia species to test for topological congruence. The results showed some cases of phylogenetic incongruence due to misplacement of four haplotypes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heteroplasmy; Sylvatic vectors; Chagas disease; Gajardoi; Spinolai; Cytochrome.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300390

Transmission analysis of candidate genes for nonsyndromic oral clefts in Brazilian parent-child triads with recurrence
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Aline Lourenço da; Ribeiro,Lucilene Arilho; Cooper,Margaret E; Marazita,Mary L; Moretti-Ferreira,Danilo.
Cleft lip and/or palate (CL/P) is a major congenital defect with complex etiology, including multiple genetic and environmental factors. Approximately two thirds of the cases are not accompanied by other anomalies and are called nonsyndromic (NS). In the present study, we performed transmission distortion analysis of the MSX1-CA, TGFB3-CA and MTHFR-C677T polymorphisms in 60 parent-child triads, in which the NS-CL/P affected child had at least one affected parent. No association with genes MSX1 or TGFB3 was found, but the results were suggestive of an association of the MTHFR-C677T polymorphism with NS-CL/P.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral clefts; Cleft lip; Cleft palate.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300005

Environmental interaction, additive and non-additive genetic variability is involved in the expression of tissue and whole-plant heat tolerance in upland cotton (Gossypium hirsutum. L)
Provedor de dados: Genetics and Molecular Biology
Autores: Hafeez-ur-Rahman,.
Heat tolerance is measured at tissue level by cellular membrane thermostability (CMT) and at the whole plant level by the heat tolerance index (HTI). Eight upland cotton cultivars and 15 crosses were used to determine the type and extent of genetic variability associated with the expression of these traits between and within environments. Heat stress and non-stress conditions were used as the CMT environments and years for HTI. The wide variation in heterotic expression and combining ability effects observed for CMT and HTI suggest multigenic inheritance of these traits. Significant genetic variability across environments was evident but the traits were not highly heritable because of substantial environmental interaction. The available genetic variability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cotton; Environmental interaction; Genetic variability; Heat tolerance; Membrane stability.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300022

CYP21 gene mutations in Brazilian patients with 21-hydroxylase deficiency from the Amazon region
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Tarcísio André Amorim de; Souza,Izabel Cristina Neves de; Yoshioka,France Keiko Nascimento; Caldato,Milena Coelho Fernandes; Torres,Nilza Nei; Garcia,Lena Stilianidi; Guerreiro,João Farias.
Congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency (P450c21, CYP21) accounts for about 95% of all CAH cases. The incidence of CYP21 gene mutations has been extensively studied in the last years, but in Brazil it has been investigated only in Southeast Brazilian patients. This study is the first report on the distribution of CYP21 mutations in patients from the Amazon region. Direct sequencing of the CYP21 gene identified at least one mutation in 96% of the studied chromosomes. The most common mutations found were IVS2-13A/C &gt; G (36%), Q318X (12%), V281L (12%), 1760_1761insT (9%), Cluster E6 (7%), and P30L (7%). The worldwide most common mutations were identified among patients from the Amazon region at frequencies that may be expected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon region; 21-hydroxylase deficiency; DNA sequencing.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400004

Improvement in genetic characteristics and oil yield of selected soybean progenies from octuple crosses
Provedor de dados: Genetics and Molecular Biology
Autores: Hamawaki,Osvaldo Toshiyuki; Vello,Natal Antônio; Didoné,Claudinei Antonio.
The objective of the present study was to evaluate 44 soybean octuple crosses in the F4:3[8] and F5:3[8] generations in order to select progenies superior for seed oil yield (OY) and other important agronomic characteristics. Octuple crosses were hybridized in a chain mating system. In one group, crosses were carried out for three generations with the adapted x exotic parents until octuple crosses with 75% adapted genes and 25% exotic genes were obtained. In a second group, hybridization of adapted x adapted parents originated crosses with 100% adapted genes. During the growing season 1994/95, the progenies F4:3[8] were evaluated by using the augmented block design. The progenies F5:3[8] were evaluated during the growing season 1995/96 in three experiments...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400026

Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi
Provedor de dados: Genetics and Molecular Biology
Autores: Nora,Luísa Czamanski; Gonçales,Relber Aguiar; Martins-Santana,Leonardo; Ferreira,Beatriz Henriques; Rodrigues,Fernando; Silva-Rocha,Rafael.
Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resistance to Hygromicin B flanked by alternate promoters, one for yeast and another for filamentous fungi. Left and right borders were added for Agrobacterium tumefaciens recognition, and a minimal broad-host range RK2 replication origin. Transformation was validated in the pathogenic fungus Paracoccidioides lutzii. Hence, we developed an efficient and reliable molecular tool for fungal transformation:...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Synthetic biology; Fungi; Agrobacterium tumefaciens; Transformation; Vectors.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300395

The use of random amplified polymorphic DNA to evaluate the genetic variability of Ponkan mandarin (Citrus reticulata Blanco) accessions
Provedor de dados: Genetics and Molecular Biology
Autores: Coletta Filho,Helvécio Della; Machado,Marcos Antonio; Targon,M. Luiza P.N.; Pompeu Jr.,Jorgino.
RAPD analysis of 19 Ponkan mandarin accessions was performed using 25 random primers. Of 112 amplification products selected, only 32 were polymorphic across five accessions. The absence of genetic variability among the other 14 accessions suggested that they were either clonal propagations with different local names, or that they had undetectable genetic variability, such as point mutations which cannot be detected by RAPD.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100031

The glyceraldehyde-3-phosphate dehydrogenase gene of Moniliophthora perniciosa, the causal agent of witches' broom disease of Theobroma cacao
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Juliana O.; Pereira,Jorge F.; Rincones,Johana; Barau,Joan G.; Araújo,Elza F.; Pereira,Gonçalo A.G.; Queiroz,Marisa V.
This report describes the cloning, sequence and expression analysis of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene of Moniliophthora perniciosa, the most important pathogen of cocoa in Brazil. Southern blot analysis revealed the presence of a single copy of the GAPDH gene in the M. perniciosa genome (MpGAPDH). The complete MpGAPDH coding sequence contained 1,461 bp with eight introns that were conserved in the GAPDH genes of other basidiomycete species. The cis-elements in the promoter region of the MpGAPDH gene were similar to those of other basidiomycetes. Likewise, the MpGAPDH gene encoded a putative 339 amino acid protein that shared significant sequence similarity with other GAPDH proteins in fungi, plants, and metazoans. Phylogenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glyceraldehyde-3-phosphate dehydrogenase gene; Moniliophthora perniciosa; Witches' broom.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200024

Genetic counseling follow-up - a retrospective study with a quantitative approach
Provedor de dados: Genetics and Molecular Biology
Autores: De Pina-Neto,João M.; Petean,Eucia Beatriz L.
The impact of genetic counseling (GC) was evaluated in families, who were interviewed at least two and half years and at most seven years after GC at the Genetics Service of the University Hospital, Faculty of Medicine of Ribeirão Preto, University of São Paulo (HC, FMRP, USP). The 113 families interviewed in this study were asked 48 questions and all children born after GC were studied clinically. We evaluated the families for spontaneous motivation for GC and understanding of GC information, their reproductive decisions, changes in the family after GC and the health status of new children. The majority of families seen at the Hospital das Clínicas de Ribeirão Preto were not spontaneously motivated to undergo GC. They had a low level of understanding...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300003

Karyotype and nuclear DNA content of Trichomycterus areolatus (Siluriformes, Trichomycteridae)
Provedor de dados: Genetics and Molecular Biology
Autores: Colihueque,Nelson; Corrales,Olga; Parraguez,Margarita.
Cytogenetic analysis of Trichomycterus areolatus, collected from the Tijeral and Huilma Rivers in southern Chile has shown a diploid chromosome number of 2n = 54, a fundamental number of FN = 106, and a karyotypic formula of 44m + 8sm + 2st. Intra-individual polymorphism of chromosome number (2n = 54, 55 and 56) in specimens from the Huilma River has also been documented, providing further evidence of the occurrence of this phenomenon in Trichomycterus. The karyotype exhibited large chromosome pairs: metacentric pairs 1 (relative length 7.54%), 2 (5.75%) and 3 (5.09%), submetacentric pair 23 (5.25%), and subtelocentic pair 27 (5.28%). Nuclear DNA content analysis showed an average value of 5.04 ± 1.09 pg/nucleus. This DNA content is higher than the mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Nuclear DNA content; Trichomycterus; Siluriformes.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200014

Insights into the structure of the subtelomeric chromosome segments
Provedor de dados: Genetics and Molecular Biology
Autores: Drets,Máximo E.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400056

Phylogenetic correlograms and the evolution of body size in South American owls (Strigiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz-Filho,José Alexandre Felizola; Sant'Ana,Carlos Eduardo Ramos de.
During the last few years, many models have been proposed to link microevolutionary processes to macroevolutionary patterns, defined by comparative data analysis. Among these, Brownian motion and Ornstein-Uhlenbeck (O-U) processes have been used to model, respectively, genetic drift or directional selection and stabilizing selection. These models produce different curves of pairwise variance between species against time since divergence, in such a way that different profiles appear in phylogenetic correlograms. We analyzed variation in body length among 19 species of South American owls, by means of phylogenetic correlograms constructed using Moran's I coefficient in four distance classes. Phylogeny among species was based on DNA hybridization. The...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200008

The prion protein and New World primate phylogeny
Provedor de dados: Genetics and Molecular Biology
Autores: Schneider,Igor; Schneider,Horacio; Schneider,Maria Paula; Silva,Artur.
The PrP C prion protein contains 250 amino acids with some variation among species and is expressed in several cell types. PrP C is converted to PrP Sc by a post-translational process in which it acquires amino acid sequences of three-dimensional conformation of beta-sheets. Variations in the prion protein gene were observed among 16 genera of New World primates (Platyrrhini), and resulted in amino acid substitutions when compared with the human sequence. Seven substitutions not yet described in the literature were found: W -&gt; R at position 31 in Cebuella, T -&gt; A at position 95 in Cacajao and Chiropotes, N-&gt; S at position 100 in Brachyteles, L -&gt; Q at position 130 in Leontopithecus (in the sequence responsible for generating the beta-sheet 1),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prion protein; New World monkeys; Molecular phylogeny.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400007

How strong was the bottleneck associated to the peopling of the Americas? New insights from multilocus sequence data
Provedor de dados: Genetics and Molecular Biology
Autores: Fagundes,Nelson J.R.; Tagliani-Ribeiro,Alice; Rubicz,Rohina; Tarskaia,Larissa; Crawford,Michael H.; Salzano,Francisco M.; Bonatto,Sandro L.
Abstract In spite of many genetic studies that contributed for a deep knowledge about the peopling of the Americas, no consensus has emerged about important parameters such as the effective size of the Native Americans founder population. Previous estimates based on genomic datasets may have been biased by the use of admixed individuals from Latino populations, while other recent studies using samples from Native American individuals relied on approximated analytical approaches. In this study we use resequencing data for nine independent regions in a set of Native American and Siberian individuals and a full-likelihood approach based on isolation-with-migration scenarios accounting for recent flow between Asian and Native American populations. Our results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolation with Migration model; Coalescent analysis; Native Siberians.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200206

Enhanced tethered-flight duration and locomotor activity by overexpression of the human gene SOD1 in Drosophila motorneurons
Provedor de dados: Genetics and Molecular Biology
Autores: Petrosyan,Agavni; Hsieh,I-Hui; Phillips,John P.; Saberi,Kourosh.
Mutation of the human gene superoxide dismutase (hSOD1) is associated with the fatal neurodegenerative disease familial amyotrophic lateral sclerosis (Lou Gehrig’s disease). Selective overexpression of hSOD1 in Drosophila motorneurons increases lifespan to 140% of normal. The current study was designed to determine resistance to lifespan decline and failure of sensorimotor functions by overexpressing hSOD1 in Drosophila‘s motorneurons. First, we measured the ability to maintain continuous flight and wingbeat frequency (WBF) as a function of age (5 to 50 days). Flies overexpressing hSOD1 under the D42-GAL4 activator were able to sustain flight significantly longer than controls, with the largest effect observed in the middle stages of life. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Behavioral genetics; Superoxide dismutase; Drosophila; Motorneuron; Longevity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100107

The Euclidean Steiner Ratio and the measure of chirality of biomacromolecules
Provedor de dados: Genetics and Molecular Biology
Autores: Mondaini,R. P.
The study of Euclidean Steiner Trees is one of the alternative methods to unveil Nature's plans for the internal architecture of biomacromolecules. Recently, the minimum surface structure of the A-DNA and of the Tobacco Mosaic Virus was shown to be described by a "strake" surface. These results have been substantiated by an explicit calculation of the Steiner Ratio Function in a very restrictive modelling scheme. In the present work, we also introduce the measure of chirality as an essential part of a thermodynamical approach to model biomolecular structure. In a certain sense, the Steiner Ratio function is constrained by the chirality measure to assume a value dictated by Nature. This value is a measure of the free energy of the molecular configuration.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Steiner Ratio; Chirality; Upper bound.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400030

Sciaenidae fish of the Caeté River estuary, Northern Brazil: mitochondrial DNA suggests explosive radiation for the Western Atlantic assemblage
Provedor de dados: Genetics and Molecular Biology
Autores: Vinson,Christina; Gomes,Grazielle; Schneider,Horacio; Sampaio,Iracilda.
Sciaenids are fish which are normally abundant in tropical estuaries of the western Atlantic. Studies on the Caeté river estuary in the northern Brazilian state of Pará have revealed that in this area Sciaenidae is the dominant family, comprising almost 50% of all teleosts sampled. In this paper we present the results of the first phylogenetic study on South American estuarine sciaenids, during which we obtained mitochondrial gene 16S sequences from 15 species belonging to eight genera occurring in the Caeté estuary. Intergeneric nucleotide divergences varied from 5 to 15%, Lonchurus and Menticirrhus being the most divergent lineages. Nucleotide divergences were quite variable amongst species of the same genus, ranging from 1.2% (Stellifer microps x...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sciaenidae; Perciformes; Mitochondrial DNA; RRNA 16S; Caeté River estuary.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200008

Araucaria angustifolia chloroplast genome sequence and its relation to other Araucariaceae
Provedor de dados: Genetics and Molecular Biology
Autores: Brandão,José Henrique S. G.; Rodrigues,Nureyev F.; Eguiluz,Maria; Guzman,Frank; Margis,Rogerio.
Abstract Araucaria angustifolia is endemic to southern Brazil. Known as Brazilian pine, A. angustifolia is the only native conifer species with economic and social relevance in this country. Due to massive exploitation, it has suffered a significant population decline and currently is classified as critically endangered. This encouraged the scientific community to investigate genetic features in Brazilian pine to increase resources for management and preservation. In this work, RNA-Seq data was used to determine the complete nucleotide sequence of the A. angustifolia chloroplast genome (cpDNA). The cpDNA is 146,203 bp in length and contains 122 genes, including 80 protein-coding genes, 5 ribosomal RNA genes, and 37 tRNA genes. Coding regions comprise...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian pine; Plastid; Genome; CpDNA; Conservation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400671

Identification of 14-3-3-like protein in sugarcane (Saccharum officinarum)
Provedor de dados: Genetics and Molecular Biology
Autores: Kuramae,Eiko Eurya; Fenille,Roseli Chela; Rosa Jr.,Vicente Eugenio de.
In a search of the sugarcane expressed sequence tag (SUCEST) database we located three full-length cDNAs (SCCCLR1022D05.g, SCCCRZ1001D02.g and SCBFLR1026E02.g) encoding the 14-3-3 proteins from sugarcane (Saccharum officinarum). The encoded proteins were identified based on the clustering of the expressed sequence tags and were shown to encode proteins similar to 14-3-3 proteins of other monocotyledonous plants. Cluster SCCCLR1022D05.g was 99% similar to the maize 14-3-3-like protein (gi|1345587) while cluster SCCCRZ1001D02.g shared 96% and SCBFLR1026E02.g 94% similarity with the 14-3-3 protein of rice (gi|7435022). Although 14-3-3 proteins have been reported to be specific to particular species, tissue or organ from which they were isolated, all three...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100007

Genetics of naked grain oats in crosses with Brazilian genotypes
Provedor de dados: Genetics and Molecular Biology
Autores: Cabral,Cândida Braga; Milach,Sandra Cristina Kothe; Federizzi,Luiz Carlos; Bothona,Cristhiane Abegg; Taderka,Ivone; Tisian,Luis Marcelo; Limberger,Emerson.
Six crosses between three naked oat (Avena sativa L.) germplasm sources from the USA and Australia and three Brazilian hulled genotypes were studied for the segregation of naked grain and multiflorous spikelet traits in the F2 and F3 generations. One major gene with incomplete dominance and expressivity for both traits was identified in all populations. The occurrence of intermediate types for naked grain varied with the population and did not depend on the germplasm source of naked grain used. The frequency of intermediate types varied among F2 populations indicating that selection for higher expressivity of naked grain could be used to develop Brazilian oat lines with naked grain.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400025

Association of IGF-I gene polymorphisms with milk yield and body size in Chinese dairy goats
Provedor de dados: Genetics and Molecular Biology
Autores: Deng,Chanjuan; Ma,Rongnuan; Yue,Xiangpeng; Lan,Xianyong; Chen,Hong; Lei,Chuzhao.
The association of IGF-I gene polymorphisms with certain traits in 708 individuals of two Chinese dairy-goat breeds (Guanzhong and Xinong Saanen) was investigated. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing methods were employed in screening for genetic variation. Two novel mutations were detected in the 5'-flanking region and in intron 4 of IGF-I gene, viz., g.1617 G &gt; A and g.5752 G &gt; C (accession D26119.2), respectively. The associations of the g.1617 G &gt; A mutation with milk yield and the body size were not significant (p &gt; 0.05). However, in the case of g.5752 G &gt; C, Xinong Saanen dairy goats with the CG genotype presented longer bodies (p &lt; 0.05). Chest circumference (p &lt; 0.05)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IGF-I gene; Dairy goat; Milk yield; PCR- SSCP.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200012

The second of a series of articles for the 60th anniversary of the Brazilian Society of Genetics
Provedor de dados: Genetics and Molecular Biology
Autores: Salzano,Francisco Mauro; Santos,Fabrício Rodrigues dos; Hartfelder,Klaus; Menck,Carlos F.M.
Tipo: Info:eu-repo/semantics/other
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400475

Nucleolar organizer regions in Sittasomus griseicapillus and Lepidocolaptes angustirostris (Aves, Dendrocolaptidae): evidence of a chromosome inversion
Provedor de dados: Genetics and Molecular Biology
Autores: Barbosa,Marcelo de Oliveira; Silva,Rubens Rodrigues da; Correia,Vanessa Carolina de Sena; Santos,Luana Pereira dos; Garnero,Analía del Valle; Gunski,Ricardo José.
Cytogenetic studies in birds are still scarce compared to other vertebrates. Woodcreepers (Dendrocolaptidae) are part of a highly specialized group within the Suboscines of the New World. They are forest birds exclusive to the Neotropical region and similar to woodpeckers, at a comparable evolutionary stage. This paper describes for the first time the karyotypes of the Olivaceous and the Narrow-billed Woodcreeper using conventional staining with Giemsa and silver nitrate staining of the nucleolar organizer regions (Ag-NORs). Metaphases were obtained by fibular bone marrow culture. The chromosome number of the Olivaceous Woodcreeper was 2n = 82 and of the Narrow-billed Woodcreeper, 2n = 82. Ag-NORs in the largest macrochromosome pair and evidence of a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Woodcreepers; Ag-NOR; Chromosomal inversion.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100010

Increased expression levels of Syntaxin 1A and Synaptobrevin 2/Vesicle-Associated Membrane Protein-2 are associated with the progression of bladder cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Raja,Sadaf Azad; Abbas,Seher; Shah,Syed Tahir Abbas; Tariq,Aamira; Bibi,Nazia; Yousuf,Arzu; Khawaja,Athar; Nawaz,Muhammad; Mehmood,Arshad; Khan,Muhammad Jadoon; Hussain,Alamdar.
Abstract Gene expression is tightly regulated in time and space through a multitude of factors consisting of signaling molecules. Soluble N-ethylmaleimide-sensitive-factor attachment protein receptors (SNARE) are membrane proteins responsible for the intercellular trafficking of signals through endocytosis and exocytosis of vesicles. Altered expression of SNARE proteins in cellular communication is the major hallmark of cancer phenotypes as indicated in recent studies. SNAREs play an important role in maintaining cell growth and epithelial membrane permeability of the bladder and are not only involved in cancer progression but also metastatic cell invasion through SNARE-mediated trafficking. Synaptobrevin2/Vesicle associated membrane protein-2 (v-SNARE)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SNARE; Bladder cancer; Vesicle fusion; Gene expression.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100040

RAPD fingerprinting of the ornamental fish Badis badis (Hamilton 1822) and Dario dario (Kullander and Britz 2002) (Perciformes, Badidae) from West Bengal, India
Provedor de dados: Genetics and Molecular Biology
Autores: Brahmane,Manoj P.; Mitra,Krishna; Mishra,Sudhanshu S.
We used random amplification of polymorphic DNA (RAPD) to generate species-specific diagnostic fragment patterns for the molecular identification of the ornamental aquarium fish species Badis badis and Dario dario. Seven arbitrary oligodecamer primers produced a total of 116 bands of which 98.23% were polymorphic. The size of the amplified products was in the range 340 bp to 2170 bp. Intraspecies genetic similarity was 0.879 ± 0.023 for B. badis and 0.840 ± 0.014 for D. dario while interspecies genetic similarity was 0.602 ± 0.017, with cluster analysis displaying separate taxonomic and evolutionary status for these fish. The results show that RAPD was useful for the molecular identification of aquarium fish species, with morphological traits also being...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Badis badis; Dario dario; Diagnostic markers; RAPD molecular identification; West Bengal.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400028

A two-step strategy for the complementation of M: tuberculosis mutants
Provedor de dados: Genetics and Molecular Biology
Autores: Movahedzadeh,Farahnaz; Frita,Rosangela; Gutka,Hiten J.
The sequence of Mycobacterium tuberculosis, completed in 1998, facilitated both the development of genomic tools, and the creation of a number of mycobacterial mutants. These mutants have a wide range of phenotypes, from attenuated to hypervirulent strains. These phenotypes must be confirmed, to rule out possible secondary mutations that may arise during the generation of mutant strains. This may occur during the amplification of target genes or during the generation of the mutation, thus constructing a complementation strain, which expresses the wild-type copy of the gene in the mutant strain, becomes necessary. In this study we have introduced a two-step strategy to construct complementation strains using the Ag85 promoter. We have constitutively...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Mutagenesis; Constitutive expression; Complementation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200020

In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Lima-Rezende,Cássia Alves; Fernandes,Gislaine Aparecida; Silva,Helder Elias da; Dobkowski-Marinho,Sarah; Santos,Victor Fernandes; Rodrigues,Fernando Pacheco; Caparroz,Renato.
Abstract The illegal trade is a major threat to many bird species, and parrots are common victims of this activity. Domestic and international pet markets are interested on different parrot species, such as the Blue-and-yellow Macaw (Ara ararauna). This South American macaw is not globally threatened, but is under protection from over-exploitation. This study aimed to identify and characterize novel microsatellite loci for population and parentage analysis of A. ararauna. Scaffold sequences of Ara macao available in the NCBI database were used for microsatellite searches using MsatCommander software. We tested a total of 28 loci, from which 25 were polymorphic, one was monomorphic, and two did not generated amplification products. For polymorphic loci, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Captive breeding; Illegal wildlife trade; Parentage; Psittacidae; Wildlife forensics.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100068

Boundaries in metagenomic screenings using lacZα-based vectors
Provedor de dados: Genetics and Molecular Biology
Autores: Alves,Luana de Fátima; Borelli,Tiago Cabral; Westmann,Cauã Antunes; Silva-Rocha,Rafael; Guazzaroni,María-Eugenia.
Abstract Metagenomics approaches have been of high relevance for providing enzymes used in diverse industrial applications. In the current study, we have focused on the prospection of protease and glycosyl hydrolase activities from a soil sample by using the lacZα -based plasmid pSEVA232. For this, we used a functional screen based on skimmed milk agar and a pH indicator dye for detection of both enzymes, as previously reported in literature. Although we effectively identified positive clones in the screenings, subsequent experiments revealed that this phenotype was not because of the hydrolytic activity encoded in the metagenomic fragments, but rather due to the insertion of small metagenomic DNA fragments in frame within the coding region of the lacZ...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional metagenomics; Protease; Glycosyl hydrolase; False positive clones.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100803

Population structure and genetic diversity of the giant anteater (Myrmecophaga tridactyla: Myrmecophagidae, Pilosa) in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Clozato,Camila L.; Miranda,Flávia R.; Lara-Ruiz,Paula; Collevatti,Rosane G.; Santos,Fabrício R.
Abstract The giant anteater (Myrmecophaga tridactyla, Pilosa, Linnaeus 1758) belongs to the mammalian order Pilosa and presents a large distribution along South America, occupying a great variety of habitats. It is listed in the IUCN Red List of threatened species as Vulnerable. Despite threatened, there is a lack of studies regarding its genetic variability. The aim of this study was to examine the genetic diversity and patterns of genetic structure within remaining populations. We analyzed 77 individuals from seven different populations distributed in four biomes across Brazil: Cerrado, Pantanal, Atlantic Forest and Amazon Forest. We sequenced two mitochondrial markers (control region and Cyt-b) and two nuclear markers (AMELY and RAG2). We found high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Giant Anteater; Xenarthra; Cerrado; Genetic diversity; Population structure.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100050

The future of plant biotechnology in a globalized and environmentally endangered world
Provedor de dados: Genetics and Molecular Biology
Autores: Montagu,Marc Van.
Abstract This paper draws on the importance of science-based agriculture in order to throw light on the way scientific achievements are at the basis of modern civilization. An overview of literature on plant biotechnology innovations and the need to steer agriculture towards sustainability introduces a series of perspectives on how plant biotech can contribute to the major challenge of feeding our super population with enough nutritious food without further compromise of the environment. The paper argues that science alone will not solve problems. Three major forces - science, the economy and society - shape our modern world. There is a need for a new social contract to harmonize these forces. The deployment of the technologies must be done on the basis of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant biotech; GMO; Sustainable agriculture; Science and society.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000300301

Genetic parameters for faecal egg count, packed-cell volume and body-weight in Santa Inês lambs
Provedor de dados: Genetics and Molecular Biology
Autores: Lôbo,Raimundo N.B.; Vieira,Luiz S.; Oliveira,Amaury A. de; Muniz,Evandro N.; Silva,José M. da.
Worm infection is one of the main factors responsible for economic losses in sheep breeding in Brazil. Random regression analysis was used to estimate genetic parameters for the factors faecal egg-count (FEC), packed-cell volume (PCV) and body weight (BW) in Santa Inês lambs. Data from 119 female, offspring of nine rams, were collected between December, 2005 and December, 2006, from the experimental flock of Embrapa Tabuleiros Costeiros, the Brazilian Agricultural Research Corporation located in Frei Paulo, SE, Brazil. After weaning, females were drenched until the faecal egg count had dropped to zero. Two natural challenges were undertaken. FEC heritability was extremely variable, this increasing from 0.04 to 0.27 in the first challenge and from 0.01 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic correlations; Heritability; Legendre polynomials; Random regression.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200014

Electrophoretic characterization of Aspergillus nidulans strains with chromosomal duplications
Provedor de dados: Genetics and Molecular Biology
Autores: Queiroz,Marisa V. de; Pizzirani-Kleiner,Aline Aparecida; Azevedo,João Lúcio.
Pulsed-field gel electrophoresis was used to characterize strains of Aspergillus nidulans with a chromosomal duplication Dp(I-II). Morphologically deteriorated and improved variants of these strains were also analyzed. The electrophoretic karyotype demonstrated that in two duplicated strains (A and B) the 4.2 Mb band, which corresponds to chromosome II, was absent and a new band was observed. Hybridization studies using the uapA (chromosome I) and wA (chromosome II) genes demonstrated that the new band corresponded to chromosome II plus the duplicated segment of chromosome I. The size of the chromosomal duplication was approximately 1.0 Mb. Analysis of the chromosomal bands of a morphologically improved strain showed that the duplicated segment of...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200009

Use of AFLP and RAPD molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the Patagonian Bromus setifolius complex
Provedor de dados: Genetics and Molecular Biology
Autores: García,Ana M.; Schrauf,Gustavo E.; González,Graciela; Poggio,Lidia; Naranjo,Carlos A.; Dupal,Marck P.; Spangenberg,Germán C.; Forster,John W.
Bromus setifolius var. pictus (Hook) Skottsb., B. setifolius var. setifolius Presl. and B. setifolius var. brevifolius Ness are three native Patagonian taxa in the section Pnigma Dumort of the genus Bromus L. AFLP and RAPD analysis, in conjunction with genetic distance measurements and statistical techniques, revealed variation within this group and indicated that B. setifolius var. brevifolius was closely related to B. setifolius var. pictus, with both taxa being more distantly related to B. setifolius var. setifolius. Cytogenetic analysis confirmed the chromosomal number of B. setifolius var. pictus (2n = 70) and B. setifolius var. setifolius (2n = 28) and showed for the first time that B. setifolius var. brevifolius had 2n = 70. The combination of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Argentina; Bromus setifolius complex; Cytogenetics; Patagonia; RAPD.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200018

Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia
Provedor de dados: Genetics and Molecular Biology
Autores: Chen,Liang; Xia,Hengchuan; Wang,Yiting; Chen,Keping; Qin,Lvgao; Wang,Bin; Yao,Qin; Li,Jun; He,Yuanqing; Zhao,Ermi.
Snake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS). We also constructed a local protein sequence database based on transcriptome sequencing to facilitate protein identification. Of the 268 protein spots revealed by 2-DE 109 gave positive MS signals, 84 of which were identified by searching the NCBInr, Swiss-Prot and local databases. The other 25 protein spots could not be identified, possibly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2D electrophoresis; Elaphe taeniura; MALDI-TOF mass spectrometry; Protein.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300020

Highly polymorphic DNA markers in an Africanized honey bee population in Costa Rica
Provedor de dados: Genetics and Molecular Biology
Autores: Lobo Segura,Jorge Arturo.
Two genetic markers (the mtDNA COI-COII intergenic region and the microsatellite A7) with high levels of variability in South African and European honey bees were analyzed in wild swarms of Africanized honey bees (Apis mellifera) from Costa Rica. Allelic or haplotypic frequencies revealed high levels of genetic variability at these loci in this population. Most of the alleles were African alleles, although some European-derived alleles were also present. Differences in the frequencies of African alleles between African and Africanized samples were minor, which could be explained by founder effects occurring during the introduction of African honey bee populations into South America.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200013

Nine cycles of mass selection for increasing oil content in two maize (Zea mays L.) synthetics
Provedor de dados: Genetics and Molecular Biology
Autores: Rosulj,Milorad; Trifunovic,S.; Husic,I.
The objectives of this study were to estimate changes in oil content, grain yield, percentage of broken plants and changes in yield components in the maize populations DS7u and YuSSSu. As estimations were performed at C0 and C9 for both populations, it was possible to observe changes occurring following long-term mass selection for high oil content. The synthetic population DS7u population was developed by recombination of 29 inbred lines of Yugoslav, Canadian and US origin. The synthetic population YuSSSu population is an Iowa Stiff Stalk Synthetic - BSS(R)C5. Progenies were derived according to the North Carolina Design II. Results indicated that nine cycles of selection led to statistically significant increase in oil content and statistically...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corn; Mass selection; High oil populations; Components of genetic variance; Additive and phenotypic correlations.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400015

Chromosomal inversion polymorphism in Drosophila mediopunctata: seasonal, altitudinal, and latitudinal variation
Provedor de dados: Genetics and Molecular Biology
Autores: Ananina,Galina; Peixoto,Alexandre A.; Bitner-Mathé,Blanche C.; Souza,Wilma N.; Silva,Luciano Basso da; Valente,Vera L.S.; Klaczko,Louis B.
The most polymorphic chromosome for inversions in Drosophila mediopunctata is the chromosome II, where 17 inversions have been found, eight of which occurring in the distal region and nine in the proximal region. We present an analysis of the chromosome II inversion polymorphism with respect to seasonal, altitudinal and latitudinal variation. In D. mediopunctata from the Parque Nacional do Itatiaia (southeastern Brazil), the frequencies of three of the distal inversions (namely DA, DS, and DP) vary seasonally. These inversions also show altitudinal clines in their frequencies. This microgeographic pattern was not observed on a macrogeographic scale. D. mediopunctata from Porto Alegre are less polymorphic for inversions than other populations, the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cline; Microgeographic variation; Temperature; Tripunctata group; Brazil.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100011

Development of a quantitative competitive reverse transcriptase polymerase chain reaction for the quantification of growth hormone gene expression in pigs
Provedor de dados: Genetics and Molecular Biology
Autores: Franco,Maurício Machaim; Almeida,Juliana Franco; Souza,Guilherme Rocha Lino de; Antunes,Robson Carlos; Goulart,Luiz Ricardo.
After the advent of the genome projects, followed by the discovery of DNA polymorphisms, basic understanding of gene expression is the next focus to explain the association between polymorphisms and the level of gene expression, as well as to demonstrate the interaction among genes. Among the various techniques for the investigation of transcriptional profiling involving patterns of gene expression, quantitative PCR is the simplest analytical laboratory technique. The objective of this work was to analyze two strategies of a competitive PCR technique for the quantification of the pig growth hormone (GH) gene expression. A pair of primers was designed targeting exons 3 and 5, and two competitive PCR strategies were performed, one utilizing a specific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pig; GH gene; Gene expression; RT-PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100003

The distribution of 45S rDNA sites in bird chromosomes suggests multiple evolutionary histories
Provedor de dados: Genetics and Molecular Biology
Autores: Degrandi,Tiago Marafiga; Gunski,Ricardo José; Garnero,Analía del Valle; Oliveira,Edivaldo Herculano Correa de; Kretschmer,Rafael; Souza,Marcelo Santos de; Barcellos,Suziane Alves; Hass,Iris.
Abstract The distribution of 45S rDNA cluster in avian karyotypes varies in different aspects, such as position, number of bearer chromosomes, and bearers being macro- or microchromosomes. The present study investigated the patterns of variation in the 45S rDNA-bearer chromosomes of birds in order to understand the evolutionary dynamics of the cluster configuration and its contribution to the evolution of bird karyotypes. A total of 73 bird species were analyzed, including both published data and species for which rDNA-FISH was conducted for the first time. In most birds, the 45S rDNA clusters were located in a single pair of microchromosomes. Hence, the location of 45S rDNA in macrochromosomes, observed only in Neognathae species, seems to be a derived...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FISH; Chromosome; Chromosome evolution; Cytogenetics; Aves.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400601

Wolbachia in guilds of Anastrepha fruit flies (Tephritidae) and parasitoid wasps (Braconidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Mascarenhas,Rodrigo O; Prezotto,Leandro F; Perondini,André Luiz P; Marino,Celso Luiz; Selivon,Denise.
Abstract The endosymbiont Wolbachia is efficiently transmitted from females to their progenies, but horizontal transmission between different taxa is also known to occur. Aiming to determine if horizontal transmission might have occurred between Anastrepha fruit flies and associated braconid wasps, infection by Wolbachia was screened by amplification of a fragment of the wsp gene. Eight species of the genus Anastrepha were analyzed, from which six species of associated parasitoid wasps were recovered. The endosymbiont was found in seven Anastrepha species and in five species of braconids. The WSP Typing methodology detected eight wsp alleles belonging to Wolbachia supergroup A. Three were already known and five were new ones, among which four were found to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Fruit flies; Horizontal transmission; Wsp gene; Recombination.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400600

Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[a]pyrene and cyclophosphamide
Provedor de dados: Genetics and Molecular Biology
Autores: Araujo,Caroline de S.; Brito,Lorrane D.; Tarifa,Marina O.; Silva,Nayara J. Farah da; Rodrigues,Karoline S.; Cavalcante,Dalita G. S. M.; Gomes,Andressa S.; Zocoler,Marcos A.; Yoshihara,Eidi; Camparoto,Marjori L.; Job,Aldo E.; Kerche,Leandra E.
Abstract This study evaluated the genotoxicity, mutagenicity, antigenotoxicity, and antimutagenicity effects on biochemical parameters of oxidative stress of the Spondias dulcis bark ethanolic extract on mice. The extract was evaluated in the doses of 500, 1000, and 1500 mg/kg bw via gavage. To evaluate the protective effects of the extract, benzo[a]pyrene (B[a]P) and cyclophosphamide (CP) were chosen as DNA damage inducers. Genotoxicity and antigenotoxicity were evaluated by the comet assay. Cytotoxicity, mutagenicity, and antimutagenicity were evaluated by the micronucleus test in bone marrow and peripheral blood. The biochemical parameters of oxidative stress were evaluated by the quantification of catalase activity (CAT) and reduced glutathione (GSH)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Micronucleus test; Spondias dulcis; Cytotoxicity; Mutagenicity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400643

Association of long non-coding RNA MIAT and MALAT1 expression profiles in peripheral blood of coronary artery disease patients with previous cardiac events
Provedor de dados: Genetics and Molecular Biology
Autores: Toraih,Eman A.; El-Wazir,Aya; Alghamdi,Saleh A.; Alhazmi,Ayman S; El-Wazir,Mohammad; Abdel-Daim,Mohamed M.; Fawzy,Manal S.
Abstract Long non-coding RNAs (lncRNAs) are implicated in various cellular and pathological processes. Two lncRNAs, myocardial infarction-associated transcript (MIAT) and metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), may be involved in the pathogenesis of coronary artery disease (CAD). Here, we aimed to determine the relative circulating levels of MIAT and MALAT1 in 110 stable CAD patients and 117 controls and to correlate their levels with the clinical and laboratory data. Peripheral blood expression levels were quantified by Real-Time qPCR. The median MIAT expression level in CAD patients was significantly 12-fold higher than controls (p&lt;0.001). Otherwise, the median MALAT1 expression level was comparable in patient and control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CAD; LncRNA; MALAT1; MIAT; Real-time qPCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400509

Allelic relationships of anthracnose (Colletotrichum lindemuthianum) resistance in the common bean (Phaseolus vulgaris L.) cultivar Michelite and the proposal of a new anthracnose resistance gene, Co-11
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves-Vidigal,Maria Celeste; Silva,Claudete Rosa da; Vidigal Filho,Pedro Soares; Gonela,Adriana; Kvitschal,Marcus Vinícius.
The genetic resistance of Phaseolus vulgaris L. cultivar Michelite to races 8 and 64 of Colletotrichum lindemuthianum, causal agent of bean anthracnose, was characterized. Crosses were made between Michelite and Mexico 222 cultivars and the F2 population was inoculated with race 64 in order to study the inheritance of resistance to anthracnose in Michelite. The segregation of F2 population fitted in a ratio of 3R:1S, showing the presence of a dominant gene in Michelite gene conditioning resistance to race 64. Allelism tests were conducted with F2 populations derived from crosses between Michelite and AB 136, AND 277, BAT 93, Cornell 49-242, G 2333, Kaboon, Mexico 222, Michigan Dark Red Kidney (MRDK), Ouro Negro, Perry Marrow, PI 207262, TO, TU, and Widusa....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colletotrichum lindemuthianum; Gene pool; Mesoamerican resistance gene; Phaseolus vulgaris L.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400015

Stable silencing of β-lactoglobulin (BLG) gene by lentivirus-mediated RNAi in goat fetal fibroblasts
Provedor de dados: Genetics and Molecular Biology
Autores: Zhang,Shumin; Xiong,Kai; Xie,Zhourui; Nan,Wenting; Liu,Honglin; Chen,Jie.
β-lactoglobulin (BLG), a dominant allergen in goat milk, is difficult to remove by traditional biochemical methods. Its elimination from goat milk by genetic modification therefore poses a major challenge for modern goat breeders. A shRNA targeting BLG mRNA with high interference efficiency was identified, with which lentiviral vectors were used for mediating stable shRNA interference in goat-fetal fibroblast cells. Apart from high efficiency in the knockdown of BLG expression in these cells, lentivector-mediated RNAi manifested stable integration into the goat genome itself. Consequently, an in vitro model for goat BLG-content control was compiled, and a goat-cell line for accompanying transgenetic goat production created.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNAi; ShRNA; Lentivirus; BLG; Goat.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400020

Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair
Provedor de dados: Genetics and Molecular Biology
Autores: Wits,Maria Inês; Tobin,Gabriela Cabanas; Silveira,Maiele Dornelles; Baja,Karine Gehlen; Braga,Luisa Maria Macedo; Sesterheim,Patricia; Camassola,Melissa; Nardi,Nance Beyer.
Abstract Cell therapy and tissue engineering have been intensively researched for repair of articular cartilage. In this study, we investigated the chondrogenic potential of canine adipose-derived mesenchymal stromal cells (ASCs) combined to high molecular weight hyaluronic acid (HA) in vitro, and their therapeutic effect in dogs with chronic osteoarthritis (OA) associated with bilateral hip dysplasia. Canine ASCs were characterized after conventional 2D culture or 3D culture in HA, showing adequate immunophenotype, proliferation and trilineage differentiation, as well as chondrogenesis after cultivation in HA. ASC/HA constructs were used to treat 12 dogs with OA, sequentially assigned to control, ASC and ASC/HA groups. Animals were examined for clinical,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stromal cells; Hyaluronic acid; Osteoarthritis; Dog.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100701

A Young Seedling Stripe2 phenotype in rice is caused by mutation of a chloroplast-localized nucleoside diphosphate kinase 2 required for chloroplast biogenesis
Provedor de dados: Genetics and Molecular Biology
Autores: Zhou,Kunneng; Xia,Jiafa; Wang,Yuanlei; Ma,Tingchen; Li,Zefu.
Abstract Chloroplast development and chlorophyll (Chl) biosynthesis in plants are regulated by many genes, but the underlying molecular mechanisms remain largely elusive. We isolated a rice mutant named yss2 (young seedling stripe2) with a striated seedling phenotype beginning from leaf 2 of delayed plant growth. The mutant developed normal green leaves from leaf 5, but reduced tillering and chlorotic leaves and panicles appeared later. Chlorotic yss2 seedlings have decreased pigment contents and impaired chloroplast development. Genetic analysis showed that the mutant phenotype was due to a single recessive gene. Positional cloning and sequence analysis identified a single nucleotide substitution in YSS2 gene causing an amino acid change from Gly to Asp....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chloroplast biogenesis; NDPK; Oryza sativa; Positional cloning; YSS2 gene.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400630

A novel c.1037C &amp;gt; G (p.Ala346Gly) mutation in TP63 as cause of the ectrodactyly-ectodermal dysplasia and cleft lip/palate (EEC) syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Alves,Leandro Ucela; Pardono,Eliete; Otto,Paulo A.; Mingroni Netto,Regina Célia.
Ectrodactyly – ectodermal dysplasia and cleft lip/palate (EEC) syndrome (OMIM 604292) is a rare disorder determined by mutations in the TP63 gene. Most cases of EEC syndrome are associated to mutations in the DNA binding domain (DBD) region of the p63 protein. Here we report on a three-generation Brazilian family with three individuals (mother, son and grandfather) affected by EEC syndrome, determined by a novel mutation c.1037C &gt; G (p.Ala346Gly). The disorder in this family exhibits a broad spectrum of phenotypes: two individuals were personally examined, one presenting the complete constellation of EEC syndrome manifestations and the other presenting an intermediate phenotype; the third affected, a deceased individual not examined personally and...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: EEC syndrome; TP63-mutations; P63-associated disorders; SHFM.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100037

Evaluation of polymorphism in ten microsatellite loci in Uruguayan sheep flocks
Provedor de dados: Genetics and Molecular Biology
Autores: Tomasco,Ivanna; Wlasiuk,G.; Lessa,E.P.
The allele frequencies of 10 microsatellite loci previously described for sheep as BM1314, BM6526, OarFCB128, OarHH64, OarCP20, OarHH47, OarFCB48, OarHH35, OarHH72 and BM2508 were estimated for the Uruguayan flocks. A representative sample of 101 individuals composed by the two predominant breeds (76% Corriedale and 24% Australian Merino) was used. The sample did not show a significant tendency towards substructuring, in spite of presenting some significantly different allele frequencies between races. The Corriedale sample presents three loci in which the presence of null alleles is possible. The markers were highly variable, showing between 7 and 15 alleles each. The Polymorphism Information Content Index ranged from 0.63 to 0.87 and the Exclusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sheep; Polymorphism; Microsatellites.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100008

Meiotic behavior of economically important plant species: the relationship between fertility and male sterility
Provedor de dados: Genetics and Molecular Biology
Autores: Pagliarini,Maria Suely.
Meiosis is an event of high evolutionary stability which culminates in a reduction of chromosome number. The normal and harmonious course of meiosis ensures gamete viability. The cytologic events of gametogenesis are controlled by a large number of genes that act from premeiotic to postmeiotic mitosis. Mutations in these genes cause anomalies that may impair fertility, and many abnormalities affecting plant fertility or causing total male sterility have been detected during the evaluation of meiotic behavior in some species. Some of these abnormalities have been frequently described in the literature, while others have not been previously reported. The most frequent abnormalities found in the species analyzed were irregular chromosome segregation,...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400045

Association of the TCF7L2 rs12255372 (G/T) variant with type 2 diabetes mellitus in an Iranian population
Provedor de dados: Genetics and Molecular Biology
Autores: Alami,Faranak Mahmoudi; Ahmadi,Mehran; Bazrafshan,Hamidreza; Tabarraei,Alijan; Khosravi,Ayyoob; Tabatabaiefar,Mohammad Amin; Samaei,Nader Mansour.
In various populations worldwide, common variants of the TCF7L2 (Transcription factor 7-like 2) gene are associated with the risk of type 2 diabetes mellitus (T2DM). The aim was to investigate the association between rs12255372 (G/T) polymorphism in the TCF7L2 gene and T2DM in an Iranian population. 236 unrelated patients with T2DM, and 255 normoglycemic controls without diabetes were studied. The PCR-RFLP method was used for genotyping rs12255372 (G/T) polymorphism, and the SPSS version 18.0 for Windows for statistical analysis. The minor T allele of TCF7L2 rs12255372 was found to significantly increase the risk of T2DM, with an allelic odds ratio (OR) of 1.458 (95% CI 1.108-1.918, p = 0.007). A significant difference in TT genotype was observed between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TCF7L2 gene; Rs12255372 variant; Type 2 diabetes mellitus (T2DM); Single nucleotide polymorphism (SNP).
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300005

Genetic instability of sugarcane plants derived from meristem cultures
Provedor de dados: Genetics and Molecular Biology
Autores: Zucchi,Maria Imaculada; Arizono,Hideto; Morais,Vicente Alberto; Fungaro,Maria Helena Pelegrinelli; Vieira,Maria Lúcia Carneiro.
The RADP (Random amplified polymorphic DNA) technique was used to detect tissue-culture-induced variations in sugarcane. Plants of the Brazilian variety RB83-5486 propagated via rhizomes and via meristem cultures were studied. The polymorphism rate for 98 RAPD loci was 6.93% when the plants derived from meristems. Besides, in order to evaluate the influence of the number of subcultures on the generation of somaclonal variation, field-grown RB83-5486 plants derived from 10 meristems were studied after five subcultivations. Although different rates of polymorphism were observed, there was no direct association with the stage of subcultivation. The analysis of plants of two sugarcane varieties cultivated in vitro from meristems showed that variety RB83-5486...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sugarcane; Somaclonal variation; Meristem cultures; Phenotypic instability; RAPD.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100017

Hypothesis testing of genetic similarity based on RAPD data using Mantel tests and model matrices
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Flávia Melo; Diniz-Filho,José Alexandre Felizola; Bataus,Luiz Artur Mendes; Bastos,Rogério Pereira.
Clustering and ordination procedures in multivariate analyses have been widely used to describe patterns of genetic distances. However, in some cases, such as when dealing with Jaccard coefficients based on RAPD data, these techniques may fail to represent genetic distances because of the high dimensionality of the genetic distances caused by stochastic variation in DNA fragments among the units analyzed (species or populations). In this note, we show how Mantel tests can be used to test hypotheses about genetic distances and avoid problems with multivariate data representation. The procedure is illustrated with RAPD data comparing 20 anuran species from the cerrado, in which Jaccard genetic distances were compared to three model matrices linking the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anura; Clustering; Genetic distances; Mantel test; RAPD.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400013

Interactions in the Agrobacterium-soybean system and capability of some Brazilian soybean cultivars to produce somatic embryos
Provedor de dados: Genetics and Molecular Biology
Autores: Mauro,Antonio Orlando Di; Nóbrega,José Carlos Martins de; Mauro,Sonia Marli Z. Di; Collins,Glenn Burton.
Twenty-five Brazilian soybean cultivars were studied for susceptibility to four strains of Agrobacterium tumefaciens (C58, Ach5, Bo542 and A281) and for their ability to produce somatic embryos. Twelve plants of each cultivar were inoculated in a greenhouse at 4-6 weeks of age, using 12 inoculation sites per plant. The number of galls formed on plants were counted 8-10 weeks after inoculation. To study ability to produce somatic embryos, immature cotyledons, 4-6 mm in length, were plated onto N10 medium for induction of somatic embryogenesis, using four Petri dishes with 20 cotyledons for each cultivar. The embryogenic tissues were transferred onto new N10 medium six times at 15-day intervals and the number of somatic embryos per cultivar determined....
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100037

New insights into Phakopsora pachyrhizi infection based on transcriptome analysis in planta
Provedor de dados: Genetics and Molecular Biology
Autores: Rincão,Michelle Pires; Carvalho,Mayra Costa da Cruz Gallo de; Nascimento,Leandro Costa; Lopes-Caitar,Valéria S.; Carvalho,Kenia de; Darben,Luana M.; Yokoyama,Alessandra; Carazzolle,Marcelo Falsarella; Abdelnoor,Ricardo Vilela; Marcelino-Guimarães,Francismar Correa.
Abstract Asian soybean rust (ASR) is one of the most destructive diseases affecting soybeans. The causative agent of ASR, the fungus Phakopsora pachyrhizi, presents characteristics that make it difficult to study in vitro, limiting our knowledge of plant-pathogen dynamics. Therefore, this work used leaf lesion laser microdissection associated with deep sequencing to determine the pathogen transcriptome during compatible and incompatible interactions with soybean. The 36,350 generated unisequences provided an overview of the main genes and biological pathways that were active in the fungus during the infection cycle. We also identified the most expressed transcripts, including sequences similar to other fungal virulence and signaling proteins. Enriched P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asian soybean rust; Multigenic families; Transposable elements.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400671

Epigenetic modifications and their relation to caste and sex determination and adult division of labor in the stingless bee Melipona scutellaris
Provedor de dados: Genetics and Molecular Biology
Autores: Cardoso-Júnior,Carlos A.M.; Fujimura,Patrícia Tieme; Santos-Júnior,Célio Dias; Borges,Naiara Araújo; Ueira-Vieira,Carlos; Hartfelder,Klaus; Goulart,Luiz Ricardo; Bonetti,Ana Maria.
Abstract Stingless bees of the genus Melipona, have long been considered an enigmatic case among social insects for their mode of caste determination, where in addition to larval food type and quantity, the genotype also has a saying, as proposed over 50 years ago by Warwick E. Kerr. Several attempts have since tried to test his Mendelian two-loci/two-alleles segregation hypothesis, but only recently a single gene crucial for sex determination in bees was evidenced to be sex-specifically spliced and also caste-specifically expressed in a Melipona species. Since alternative splicing is frequently associated with epigenetic marks, and the epigenetic status plays a major role in setting the caste phenotype in the honey bee, we investigated here epigenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA methylation; Histone modification; Caste development; Social bees; Genetic caste determination.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100061

Determining the pathogenicity of CFTR missense variants: Multiple comparisons of in silico predictors and variant annotation databases
Provedor de dados: Genetics and Molecular Biology
Autores: Michels,Marcus; Matte,Ursula; Fraga,Lucas Rosa; Mancuso,Aline Castello Branco; Ligabue-Braun,Rodrigo; Berneira,Elias Figueroa Rodrigues; Siebert,Marina; Sanseverino,Maria Teresa Vieira.
Abstract Pathogenic variants in the Cystic Fibrosis Transmembrane Conductance Regulator gene (CFTR) are responsible for cystic fibrosis (CF), the commonest monogenic autosomal recessive disease, and CFTR-related disorders in infants and youth. Diagnosis of such diseases relies on clinical, functional, and molecular studies. To date, over 2,000 variants have been described on CFTR (~40% missense). Since few of them have confirmed pathogenicity, in silico analysis could help molecular diagnosis and genetic counseling. Here, the pathogenicity of 779 CFTR missense variants was predicted by consensus predictor PredictSNP and compared to annotations on CFTR2 and ClinVar. Sensitivity and specificity analysis was divided into modeling and validation phases using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CFTR; Missense variant; Prediction; Bioinformatics; Cystic fibrosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400560

Hepatic mRNA expression and plasma levels of insulin-like growth factor-I (IGF-I) in broiler chickens selected for different growth rates
Provedor de dados: Genetics and Molecular Biology
Autores: Giachetto,Poliana Fernanda; Riedel,Eduardo Correa; Gabriel,Jane Eyre; Ferro,Maria Inês Tiraboschi; Di Mauro,Sônia Marli Zingaretti; Macari,Marcos; Ferro,Jesus Aparecido.
The hepatic expression and plasma concentrations of IGF-I were investigated in three broiler chicken strains selected for different growth rates (HP-Hubbard-Pettersen, a fast growing strain; NN-Naked-neck, a strain with an intermediate growth rate and a heterozygous genotype, and C-Caipira, a slow growing crossbred strain). The chickens were studied at 1, 21 and 42 days of age and had free access to food throughout the study. Hepatic IGF-I mRNA expression was assessed by dot blot analysis using a randomly labeled chicken IGF-I cDNA as the probe and plasma IGF-I concentrations were assayed by radioimmunoassay. The hepatic levels of IGF-I mRNA increased from 1 to 21 days of age in all strains, with NN chickens showing a higher (p &lt; 0.05) IGF-I expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IGF-I mRNA; Broiler chicken; Growth performance; Plasma IGF-I.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100007

Plant arginyltransferases (ATEs)
Provedor de dados: Genetics and Molecular Biology
Autores: Domitrovic,Tatiana; Fausto,Anna K.; Silva,Tatiane da F.; Romanel,Elisson; Vaslin,Maite F. S.
Abstract Regulation of protein stability and/or degradation of misfolded and damaged proteins are essential cellular processes. A part of this regulation is mediated by the so-called N-end rule proteolytic pathway, which, in concert with the ubiquitin proteasome system (UPS), drives protein degradation depending on the N-terminal amino acid sequence. One important enzyme involved in this process is arginyl-t-RNA transferase, known as ATE. This enzyme acts post-translationally by introducing an arginine residue at the N-terminus of specific protein targets to signal degradation via the UPS. However, the function of ATEs has only recently begun to be revealed. Nonetheless, the few studies to date investigating ATE activity in plants points to the great...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ATE; N-end rule; Plant; Argyniltransferase; Protein degradation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200253

Effect of natural selection on common bean (Phaseolus vulgaris) microsatellite alleles
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Taislene Butarello; Santos,João Bosco dos.
The effect of natural selection on microsatellite simple sequence repeat (SSR) alleles was investigated in two distinct common bean (Phaseolus vulgaris) generations (F8 and F24) derived from the cross between the P. vulgaris cultivars Carioca MG x ESAL 686. The F2 segregant population was propagated by the bulk method and 107 plants were sampled in two generations (F8 and F24). Each plant generated one family which was replicated by the bulk method to F8:11 and F24:27 families from which DNA was extracted. Thirty pairs of microsatellite primers were polymorphic for the parents and the bulk of the F24:27 families. Out of 30 loci selected by natural selection, 29 microsatellite alleles came from the Carioca MG parent and one allele came from the ESAL 686...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Natural selection; Microsatellite markers; Phaseolus vulgaris; Adaptation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200024

Did Sutton and Boveri propose the so-called Sutton-Boveri chromosome hypothesis?
Provedor de dados: Genetics and Molecular Biology
Autores: Martins,L.A.-C.P.
The "Sutton-Boveri chromosome hypothesis" attempted to establish a parallel between cytological chromosome behavior and the principles followed by Mendelian factors. Today, this hypothesis is well accepted; however, it was not completely accepted at its proposal (1902-3). The aim of the present study is to elucidate the meaning of the chromosome hypothesis based on the original scientific works, taking into account the scientific context of that time. The negative treatment this hypothesis received is evaluated. I conclude that it was unfair to include Boveri's name beside that of Sutton's as one of the proponents of the chromosome hypothesis of Mendelian heredity, because he did not publish any hypothesis of that kind during the relevant period...
Tipo: Info:eu-repo/semantics/other
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200022

Biochemical and molecular characterization of 3-Methylcrotonylglycinuria in an Italian asymptomatic girl
Provedor de dados: Genetics and Molecular Biology
Autores: Cozzolino,Carla; Villani,Guglielmo RD; Frisso,Giulia; Scolamiero,Emanuela; Albano,Lucia; Gallo,Giovanna; Romanelli,Roberta; Ruoppolo,Margherita.
Abstract 3-Methylcrotonylglycinuria is an organic aciduria resulting from deficiency of 3-methylcrotonyl-CoA carboxylase (3-MCC), a biotin-dependent mitochondrial enzym carboxylating 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA during leucine catabolism. Its deficiency, due to mutations on MCCC1 and MCCC2 genes, leads to accumulation of 3-methylcrotonyl-CoA metabolites in blood and/or urine, primarily 3-hydroxyisovaleryl-carnitine (C5-OH) in plasma and 3-methylcrotonyl-glycine (3-MCG) and 3-hydroxyisovaleric acid (3-HIVA) in the urine. The phenotype of 3-MCC deficiency is highly variable, ranging from severe neurological abnormalities and death in infancy to asymptomatic adults. Here we report the biochemical and molecular characterization of an Italian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 3-Methylcrotonylglycinuria; MCCC2 mutations; 3-methylcrotonyl-CoA carboxylase deficiency; Newborn screening; Organic aciduria.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300379

Non-detection of Epstein-Barr virus and human papillomavirus in a region of high gastric cancer risk indicates a lack of a role for these viruses in gastric carcinomas
Provedor de dados: Genetics and Molecular Biology
Autores: Yuan,Xiao-yan; Wang,Ming-yi; Wang,Xiao-yan; Chang,Ai-ying; Li,Jie.
Gastric mucosa tissue was collected from patients with gastroduodenal diseases in a region of norrteastern China showing a high risk of gastric cancer incidence. The presence of EBV and HPV were assayed to investigate the relationship between gastric carcinomas and virus infection. Neither EBV nor HPV DNA was detected in tissue from the patients. The role of EBV and HPV in gastric cancer is not well understood and still needs to be clarified.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Epstein-Barr virus; Polymerase chain reaction; Gastric carcinoma.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200007

A meta-analysis of ABCG2 gene polymorphism and non-small cell lung cancer outcomes
Provedor de dados: Genetics and Molecular Biology
Autores: Fu,Lei; Wang,Rong; Yin,Ling; Shang,Xiaopu; Zhang,Runtong; Zhang,Pengjun.
Abstract We aimed to analyze the correlation between ABCG2 gene polymorphisms of 34 GG/(GA + AA) loci, 421 CC/(AC + AA) loci, and non-small cell lung cancer (NSCLC) therapeutic effects via meta-analysis. With key words, the databases PubMed and EMBASE were searched for clinical studies on ABCG2 polymorphism and NSCLC. RR and 95% CIs were used to compute combined effects, followed by heterogeneity testing. Publication bias was examined using the funnel plot method. Review Manager 5.3 software was used for the meta-analysis. Ten studies were included. No evidence of heterogeneity exists in these studies. The results indicate that two polymorphic loci of ABCG2 gene (34 G&gt;A, and 421 C&gt;A) had no relationship with the curative effect of chemotherapy for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ABCG2; Polymorphism; Non-small cell lung cancer; Chemotherapy; Meta-analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500105

Protein profile of rice (Oryza sativa) seeds
Provedor de dados: Genetics and Molecular Biology
Autores: Yang,Yanhua; Dai,Li; Xia,Hengchuan; Zhu,Keming; Liu,Haijun; Chen,Keping.
Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4-7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mass spectrometry; Proteomic analysis; Rice seed; Two-dimensional electrophoresis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100012

Genome-wide association study for birth, weaning and yearling weight in Colombian Brahman cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Martínez,Rodrigo; Bejarano,Diego; Gómez,Yolanda; Dasoneville,Romain; Jiménez,Ariel; Even,Gael; Sölkner,Johann; Mészáros,Gabor.
Abstract Genotypic and phenotypic data of 1,562 animals were analyzed to find genomic regions that potentially influence the birth weight (BW), weaning weight at seven months of age (WW) and yearling weight (YW) of Colombian Brahman cattle, with genotyping conducted using Illumina Bead chip array with 74,669 SNPs. A Single Step Genomic BLUP (ssGBLP), approach was used to estimate the proportion of variance explained by each marker. Multiple regions scattered across the genome were found to influence weights at different ages, also dependent on the trait component (direct or maternal). The most interesting regions were connected to previously identified QTLs and genes, such as ADAMTSL3, CAPN2, CAPN2, FABP6, ZEB2 influencing growth and weight traits. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bos indicus; SNP; QTL; GWAS; Body weight.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300453

Genetic variability in five populations of Partamona helleri (Hymenoptera, Apidae) from Minas Gerais State, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Borges,Andreia Arantes; Campos,Lucio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.
Partamona is a Neotropical genus of stingless bees that comprises 33 species distributed from Mexico to southern Brazil. These bees are well-adapted to anthropic environments and build their nests in several substrates. In this study, 66 colonies of Partamona helleri from five localities in the Brazilian state of Minas Gerais (São Miguel do Anta, Teixeiras, Porto Firme, Viçosa and Rio Vermelho) were analyzed using nine microsatellite loci in order to assess their genetic variability. Low levels of observed (Ho = 0.099-0.137) and expected (He = 0.128-0.145) heterozygosity were encountered and revealed discrete genetic differentiation among the populations (F ST =0.025). AMOVA further showed that most of the total genetic variation (94.24%) in P. helleri was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Partamona helleri; Population genetics; Stingless bees.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400028

Large deletion in PIGL: a common mutational mechanism in CHIME syndrome?
Provedor de dados: Genetics and Molecular Biology
Autores: Ceroni,José RM; Yamamoto,Guilherme L; Honjo,Rachel S; Kim,Chong A; Passos-Bueno,Maria R; Bertola,Débora R.
Abstract CHIME syndrome is an extremely rare autosomal recessive multisystemic disorder caused by mutations in PIGL. PIGL is an endoplasmic reticulum localized enzyme that catalyzes the second step of glycosylphosphatidylinositol (GPI) biosynthesis, which plays a role in the anchorage of cell-surface proteins including receptors, enzymes, and adhesion molecules. Germline mutations in other members of GPI and Post GPI Attachment to Proteins (PGAP) family genes have been described and constitute a group of diseases within the congenital disorders of glycosylation. Patients in this group often present alkaline phosphatase serum levels abnormalities and neurological symptoms. We report a CHIME syndrome patient who harbors a missense mutation c.500T &gt; C...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PIGL; CHIME syndrome; GPI biosynthesis; Large deletion.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100085

Population genetic analysis of Caiman crocodilus (Linnaeus, 1758) from South America
Provedor de dados: Genetics and Molecular Biology
Autores: Vasconcelos,William R.; Hrbek,Tomas; Silveira,Ronis da; Thoisy,Benoit de; Marioni,Boris; Farias,Izeni P.
The genetic structure of Caiman crocodilus was investigated using a 1085 bp mtDNA fragment of the cytochrome b gene. Inferences were based on 125 individuals from nine localities in Peru, Brazil and French Guiana. With the exception of Mamirauá Lake, Anavilhanas Archipelago and the Tapará Community which show a signal of demographic expansion, the sampled localities are in a mutation-drift genetic equilibrium. Divergence between the Amazon basin and extra-Amazon basin localities is significant; however, inference from Nested Clade Analysis cannot distinguish between continuous range expansion, long distance colonization or past fragmentation; however, past fragmentation is unlikely due to low number of mutational steps separating these two regions. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic structure; Phylogeography; Genetic diversity; Demographic expansion; Cytochrome b; Caiman crocodilus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200006

A rapid alternative technique for obtaining silver-positive patterns in chromosomes
Provedor de dados: Genetics and Molecular Biology
Autores: Kavalco,Karine Frehner; Pazza,Rubens.
Silver nitrate chromosome staining to evidence nucleolar organizer regions (NORs) is a widely adopted methodology. The aim of the present work was to improve this technique, reducing the preparation time without decreasing the quality of the results. Microwave irradiation proved to be quite efficient and reliable for this purpose, as it allowed to identify Ag-NORs equivalent to those obtained by the conventional procedure and also to reduce the concentration of the employed reagents, as well as the precipitation of debris on the preparation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silver staining; Ag-NOR; Microwaves; Chromosome banding.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200012

Chromosome instability in the murine melanoma cell line K-1735 is due to drug-specific mechanisms
Provedor de dados: Genetics and Molecular Biology
Autores: Nemeth,Margit A.; Hsu,T.C.; Pathak,S.
The purpose of the present study was to investigate chromosomal instability and DNA repair by exposing clones from the murine melanoma cell line K-1735 to the radiomimetic drug bleomycin and to the DNA polymerase a inhibitor aphidicolin. Results from previous experiments conducted with human lymphocytes have suggested synergistic chromosomal damage after simultaneous exposure to these two agents. However, in the murine cell line studied here, there was no direct correlation between the effects of these two agents. Indeed, the extensive variation in the responses to aphidicolin and bleomycin suggested different mechanisms for the repair of bleomycin-induced DNA damage by the clones. Evaluation of the unexplained propensity of some bleomycin-treated...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400010

Research Article Comparing covariance matrices: random skewers method compared to the common principal components model
Provedor de dados: Genetics and Molecular Biology
Autores: Cheverud,James M.; Marroig,Gabriel.
Comparisons of covariance patterns are becoming more common as interest in the evolution of relationships between traits and in the evolutionary phenotypic diversification of clades have grown. We present parallel analyses of covariance matrix similarity for cranial traits in 14 New World Monkey genera using the Random Skewers (RS), T-statistics, and Common Principal Components (CPC) approaches. We find that the CPC approach is very powerful in that with adequate sample sizes, it can be used to detect significant differences in matrix structure, even between matrices that are virtually identical in their evolutionary properties, as indicated by the RS results. We suggest that in many instances the assumption that population covariance matrices are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Covariance matrix; Common principal components; Random skewers; New World monkeys; Quantitative genetics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300027

Intraspecific differentiation of Chilean isolates of the entomopathogenic fungi Metarhizium anisopliae var. anisopliae as revealed by RAPD, SSR and ITS markers
Provedor de dados: Genetics and Molecular Biology
Autores: Becerra Velásquez,Viviana; Paredes Cárcamo,Mario; Rojo Meriño,Carmen; France Iglesias,Andrés; Franco Durán,Jorge.
The genus Metarhizium consists of a diverse group of asexual entomopathogenic fungi, which have a wide geographical distribution. The Chilean National Agricultural Research Institute (Instituto de Investigaciones Agropecuarias - INIA, Quilamapu Chile) has collected about 350 isolates of Metarhizium anisopliae var. anisopliae from central and southern Chile. These isolates have been partially characterized using morphological traits such as conidia size and shape, colony color, growth pattern and the efficiency of the isolates in controlling specific pests. However, further characterization with molecular markers could detect differences in DNA which could help to better understand the genetic diversity and structure of Chilean populations of this fungus....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metarhizium; Genetic diversity; RAPD; SSR; ITS markers.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100017

Effects of polymorphisms in APOB, APOE, HSD11β1, PLIN4, and ADIPOQ genes on lipid profile and anthropometric variables related to obesity in children and adolescents
Provedor de dados: Genetics and Molecular Biology
Autores: Gasparin,Caroline C.; Leite,Neiva; Tureck,Luciane V.; Souza,Ricardo L.R.; Milano-Gai,Gerusa E.; Silva,Larissa R.; Lopes,Wendell A.; Furtado-Alle,Lupe.
Abstract Genes can influence lipid profile and anthropometric variables related to obesity. The present study aimed to verify if variants of the APOE, APOB, ADIPOQ, HSD11β1, and PLIN4 genes are associated with lipid levels or anthropometric variables in a sample comprised of 393 Euro-Brazilian children and adolescents. DNA was genotyped by TaqMan allelic discrimination assay. The ε4 and ε2 alleles of the APOE gene were associated respectively with lower high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels (p=0.015 and p=0.012, respectively), while the ε3 allele was associated with higher abdominal circumference (p=0.0416) and excess weight (p=0.0001). The G allele (rs846910) of the HSD11β1 gene was also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PLIN4 gene; APOB gene; ADIPOQ gene; HSD11β1 gene; APOE gene.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500735

Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrot
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Carlos A.F.; Simon,Philipp W.
Amplified fragment length polymorphism (AFLP) is a fast and reliable tool to generate a large number of DNA markers. In two unrelated F2 populations of carrot (Daucus carota L.), Brasilia x HCM and B493 x QAL (wild carrot), it was hypothesized that DNA 1) digested with the same restriction endonuclease enzymes and amplified with the same primer combination and 2) sharing the same position in polyacrylamide gels should be conserved sequences. To test this hypothesis AFLP fragments from polyacrylamide gels were eluted, reamplified, separated in agarose gels, purified, cloned and sequenced. Among thirty-one paired fragments from each F2 population, twenty-six had identity greater than 91% and five presented identity of 24% to 44%. Among the twenty-six...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Daucus carota; Evolution; Molecular markers; SCAR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200013

Population genetics of the Chilean frog Batrachyla Leptopus (Leptodactylidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Formas,J.R.; Brieva,L.M.
Electrophoretic variation of proteins encoded by 14 loci was analyzed in eight (five continental and three insular) populations of the Chilean leptodactylid frog Batrachyla leptopus. The overall proportion of polymorphic loci was estimated to be 18.7% and the average number of alleles per locus, 1.2, while observed and expected heterozygosities were 1.7 and 5.1%, respectively. The estimated coefficient of genetic identity was 0.940; the corresponding figure for genetic distance was 0.063. F-statistics analysis showed a total inbreeding coefficient (Fit) of 0.855 and high levels of genetic subdivision (Fst = 0.596) as well as of inbreeding within populations (Fis = 0.640). However, there was only a moderate level of genetic differentiation (Fst = 0.181)...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100007

ESTs and putative line-specific (broiler and layer) SNPs identified in genes expressed in Gallus gallus pituitary and hypothalamus
Provedor de dados: Genetics and Molecular Biology
Autores: Cassoli,Clarissa Sanches da Silva; Jorge,Erika Cirstina; Patrício,Mateus; Alves,Helena Javiel; Almeida,Erik Amazonas de; Ledur,Mônica Corrêa; Coutinho,Luiz Lehmann.
Brazilian poultry industry has reached a high level of development in both meat and egg production as a result of constant technological modernization. Further improvements can be achieved through genomics, but before this can be accomplished, a better understanding of gene expression profiles and nucleotide polymorphisms is necessary. Since animal physiology is directly or indirectly controlled by the pituitary and hypothalamus, the aim of the present work was to identify and analyze genes expressed in these tissues in chicken lines with different growth potential. Two pituitary and hypothalamus cDNA libraries from 21 day broiler (TT) and layer (CC) chickens lines were constructed and allowed identification of 3,074 unique sequences and 77 single...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pituitary; Hypothalamus; EST; SNP; Chicken.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600008

Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA
Provedor de dados: Genetics and Molecular Biology
Autores: Fregonezi,Jéferson Nunes; Fernandes,Thiago; Torezan,José Marcelo Domingues; Vieira,Ana Odete S.; Vanzela,André Luís Laforga.
We studied the karyotypes of four Brazilian Cestrum species (C. amictum, C. intermedium, C. sendtnerianum and C. strigilatum) using conventional Feulgen staining, C-Giemsa and C-CMA3/DAPI banding, induction of cold-sensitive regions (CSRs) and fluorescent in situ hybridization (FISH) with rDNA probes. We found that the karyotypes of all four species was 2n = 2x = 16, with, except for the eighth acrocentric pair, a predominance of meta- and submetacentric chromosomes and various heterochromatin classes. Heterochromatic types previously unreported in Cestrum as neutral C-CMA3(0)/DAPI0 bands, CMA3+ bands not associated with NORs, and C-Giemsa/CSR/DAPI- bands were found. The heterochromatic blocks varied in size, number, position and composition. The 45S rDNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cestrum; Chromosome banding; CSRs; Karyotype evolution; RDNA probes.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100019

Numerical chromosome polymorphism in Mikania cordifolia Willd. (Asteraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Maffei,E.M.D.; Marin-Morales,M.A.; Ruas,P.M.; Ruas,C.F.
Cytogenetical analysis of Mikania cordifolia, from southeastern Brazil, using the conventional Feulgen method, showed a chromosome number of 2n = 36. Previous karyotypic descriptions for this species showed a numerical chromosome variation of 2n = 34 to 38. There was a secondary constriction in every metaphase in the first chromosome pair, which constitutes a cytological marker. Small extranumerary chromosomes with numerical variation in the same plant were found in the tenth chromosome pair.
Tipo: Info:eu-repo/semantics/other
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400023

GENOSOJA - the Brazilian soybean genome consortium: high throughput omics and beyond
Provedor de dados: Genetics and Molecular Biology
Autores: Benko-Iseppon,Ana M; Nepomuceno,Alexandre L; Abdelnoor,Ricardo V.
Tipo: Info:eu-repo/semantics/other
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200001

BRCA1 and BRCA2 rearrangements in Brazilian individuals with Hereditary Breast and Ovarian Cancer Syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Ewald,Ingrid Petroni; Cossio,Silvia Liliana; Palmero,Edenir Inez; Pinheiro,Manuela; Nascimento,Ivana Lucia de Oliveira; Machado,Taisa Manuela Bonfim; Sandes,Kiyoko Abe; Toralles,Betânia; Garicochea,Bernardo; Izetti,Patricia; Pereira,Maria Luiza Saraiva; Bock,Hugo; Vargas,Fernando Regla; Moreira,Miguel Ângelo Martins; Peixoto,Ana; Teixeira,Manuel R.; Ashton-Prolla,Patricia.
Abstract Approximately 5-10% of breast cancers are caused by germline mutations in high penetrance predisposition genes. Among these, BRCA1 and BRCA2, which are associated with the Hereditary Breast and Ovarian Cancer (HBOC) syndrome, are the most frequently affected genes. Recent studies confirm that gene rearrangements, especially in BRCA1, are responsible for a significant proportion of mutations in certain populations. In this study we determined the prevalence of BRCA rearrangements in 145 unrelated Brazilian individuals at risk for HBOC syndrome who had not been previously tested for BRCA mutations. Using Multiplex Ligation-dependent Probe Amplification (MLPA) and a specific PCR-based protocol to identify a Portuguese founder BRCA2 mutation, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Hereditary Breast and Ovarian Cancer syndrome; Gene rearrangements; BRCA gene.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200223

Eucalyptus ESTs involved in the production of 9-cis epoxycarotenoid dioxygenase, a regulatory enzyme of abscisic acid production
Provedor de dados: Genetics and Molecular Biology
Autores: Guerrini,Iraê A.; Trigueiro,Rodrigo M.; Leite,Regina M.; Wilcken,Carlos F.; Velini,Edivaldo D.; Mori,Edson S.; Furtado,Edson L.; Marino,Celso L.; Maia,Ivan G.
Abscisic acid (ABA) regulates stress responses in plants, and genomic tools can help us to understand the mechanisms involved in that process. FAPESP, a Brazilian research foundation, in association with four private forestry companies, has established the FORESTs database (&lt;A HREF="https://forests.esalq.usp.br"&gt;https://forests.esalq.usp.br&lt;/A&gt;). A search was carried out in the Eucalyptus expressed sequence tag database to find ESTs involved with 9-cis epoxycarotenoid dioxygenase (NCED), the regulatory enzyme for ABA biosynthesis, using the basic local BLAST alignment tool. We found four clusters (EGEZLV2206B11.g, EGJMWD2252H08.g, EGBFRT3107F10.g, and EGEQFB1200H10.g), which represent similar sequences of the gene that produces NCED. Data...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Eucalyptus EST; Abscisic acid; Water deficit; Drought resistance; NCED.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400020

Hidden heterochromatin: characterization in the Rodentia species Cricetus cricetus, Peromyscus eremicus (Cricetidae) and Praomys tullbergi (Muridae)
Provedor de dados: Genetics and Molecular Biology
Autores: Paço,Ana; Adega,Filomena; Guedes-Pinto,Henrique; Chaves,Raquel.
The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin revealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Constitutive heterochromatin; In situ restriction endonuclease digestion; Rodentia.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100009

Chromosomal damage and apoptosis analysis in exfoliated oral epithelial cells from mouthwash and alcohol users
Provedor de dados: Genetics and Molecular Biology
Autores: Rocha,Rodrigo dos Santos; Meireles,José Roberto Cardoso; Cerqueira,Eneida de Moraes Marcílio.
Chromosomal damage and apoptosis were analyzed in users of mouthwash and/or alcoholic beverages, using the micronucleus test on exfoliated oral mucosa cells. Samples from four groups of 20 individuals each were analyzed: three exposed groups (EG1, EG2 and EG3) and a control group (CG). EG1 comprised mouthwash users; EG2 comprised drinkers, and EG3 users of both mouthwashes and alcoholic beverages. Cell material was collected by gently scraping the insides of the cheeks. Then the cells were fixed in a methanol/acetic acid (3:1) solution and stained and counterstained, respectively, with Schiff reactive and fast green. Endpoints were computed on 2,000 cells in a blind test. Statistical analysis showed that chromosomal damage and apoptosis were significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronucleus; Apoptosis; Mouthwash; Alcoholic beverages.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500013

The expression of ABH and Lewis antigens in Brazilian semi-isolated Black communities
Provedor de dados: Genetics and Molecular Biology
Autores: Corvelo,Tereza Cristina OLiveira; Aguiar,D.C.F.; Sagica,F.E.S.
The expression of the ABH and Lewis blood groups was determined in blood and saliva samples from two semi-isolated Black communities of Northern Brazil: Cametá and Alcântara. The distributions of ABO blood group phenotypes and the ABH secretor status frequencies showed no significant differences between these populations. In contrast, there was a difference regarding the frequency of the red blood cell Le(a-b-) phenotypes, associated with erythrocyte/saliva discordance, as confirmed by the observation that individuals with Le(a-b-) red cells have the Lewis antigen in their saliva, resulting in a nongenuine Le(a-b-) phenotype, whose frequency was higher in Alcântara.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Semi-isolated black communities; Lewis blood group; ABH secretor status; Dot-blot-ELISA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300001

Population medical genetics: translating science to the community
Provedor de dados: Genetics and Molecular Biology
Autores: Giugliani,Roberto; Bender,Fernanda; Couto,Rowena; Bochernitsan,Aline; Brusius-Facchin,Ana Carolina; Burin,Maira; Amorim,Tatiana; Acosta,Angelina Xavier; Purificação,Antônio; Leistner-Segal,Sandra; Saraiva-Pereira,Maria Luiza; Jardim,Laura Bannach; Matte,Ursula; Riegel,Mariluce; Cardoso-dos-Santos,Augusto César; Rodrigues,Graziella; Oliveira,Marcelo Zagonel de; Tagliani-Ribeiro,Alice; Heck,Selia; Dresch,Vanusa; Schuler-Faccini,Lavínia; Kubaski,Francyne.
Abstract Rare genetic disorders are currently in the spotlight due to the elevated number of different conditions and significant total number of affected patients. The study of these disorders is extremely helpful for the elucidation of physiological processes related with complex disorders. Isolated populations are instrumental for the study of genetic disorders, considering their homogeneity and high proportion of affected patients in a small geographic area. These favorable conditions lead to the creation of a new discipline, known as “population medical genetics”, which integrates medical genetics, population genetics, epidemiological genetics and community genetics. In order to develop practical activities in this new discipline, the National...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population Medical Genetics; Genetic clusters; Founder effect; Population isolates.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200312

The microbiome and inborn errors of metabolism: Why we should look carefully at their interplay?
Provedor de dados: Genetics and Molecular Biology
Autores: Colonetti,Karina; Roesch,Luiz Fernando; Schwartz,Ida Vanessa Doederlein.
Abstract Research into the influence of the microbiome on the human body has been shedding new light on diseases long known to be multifactorial, such as obesity, mood disorders, autism, and inflammatory bowel disease. Although inborn errors of metabolism (IEMs) are monogenic diseases, genotype alone is not enough to explain the wide phenotypic variability observed in patients with these conditions. Genetics and diet exert a strong influence on the microbiome, and diet is used (alone or as an adjuvant) in the treatment of many IEMs. This review will describe how the effects of the microbiome on the host can interfere with IEM phenotypes through interactions with organs such as the liver and brain, two of the structures most commonly affected by IEMs. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inborn errors of metabolism; Microbiome; Microbiota; Diet; Treatment.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400515

Phylogeny and molecular dating of the cerato-platanin-encoding genes
Provedor de dados: Genetics and Molecular Biology
Autores: Yu,Hanying; Li,Lin.
The cerato-platanin family consists of proteins that can induce immune responses, cause necrosis, change chemotaxis and locomotion and may be related to the growth and development of various fungi. In this work, we analyzed the phylogenetic relationships among genes encoding members of the cerato-platanin family and computed the divergence times of the genes and corresponding fungi. The results showed that cerato-platanin-encoding genes could be classified into 10 groups but did not cluster according to fungal classes or their functions. The genes transferred horizontally and showed duplication. Molecular dating and adaptive evolution analyses indicated that the cerato-platanin gene originated with the appearance of saprophytes and that the gene was under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adaptive evolution; Cerato-platanin gene; Phylogeny; Relaxed clock.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300015

Population structure and morphometric variance of the Apis mellifera scutellata group of honeybees in Africa
Provedor de dados: Genetics and Molecular Biology
Autores: Radloff,Sarah; Hepburn,Randall.
The honeybee populations of Africa classified as Apis mellifera scutellata Lepeletier were analysed morphometrically using multivariate statistical techniques. The collection consisted of nearly 15,000 worker honeybees from 825 individual colonies at 193 localities in east Africa, extending from South Africa to Ethiopia. Factor analysis established one primary cluster, designated as A. m. scutellata. Morphocluster formation and inclusivity (correct classification) are highly sensitive to sampling distance intervals. Within the A. m. scutellata region are larger bees associated with high altitudes of mountain systems which are traditionally classified as A. m. monticola Smith, but it is evident that these bees do not form a uniform group. Variance...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200012

Quantitative trait loci for carcass, internal organ and meat quality traits on porcine chromosomes 16, 17 and 18
Provedor de dados: Genetics and Molecular Biology
Autores: Paixão,Débora M.; Silva Filho,Miguel I. da; Pereira,Mário S.; Lopes,Marcos S.; Barbosa,Leandro; Souza,Katiene Régia Silva; Lopes,Paulo S.; Guimarães,Simone E.F.
The objective of this study was to map quantitative trait loci (QTL) on porcine chromosomes 16, 17 and 18 and to determine their association with carcass, organ and meat quality traits. An F2 population was produced by crossing two boars of the naturalized Brazilian Piau breed with 18 commercial females (Landrace x Large White x Pietrain). The population was genotyped for 11 microsatellite markers distributed over the three chromosomes and the results were used to construct a marker-specific linkage map for the population. Analysis of the polymorphic information content showed that the microsatellite markers were adequate for the study of quantitative traits. QTL were identified by regression interval mapping using QTL Express software. QTL not previously...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Divergent crosses; Microsatellite markers; Pig production; QTL mapping.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500016

Heterochromatin and numeric chromosome evolution in Bignoniaceae, with emphasis on the Neotropical clade Tabebuia alliance
Provedor de dados: Genetics and Molecular Biology
Autores: Cordeiro,Joel M.P.; Kaehler,Miriam; Souza,Luiz Gustavo; Felix,Leonardo P.
Abstract Bignoniaceae is a diverse family composed of 840 species with Pantropical distribution. The chromosome number 2n = 40 is predominant in most species of the family, with n = 20 formerly being considered the haploid base number. We discuss here the haploid base number of Bignoniaceae and examine heterochromatin distributions revealed by CMA/DAPI fluorochromes in the Tabebuia alliance, as well as in some species of the Bignonieae, Tecomeae, and Jacarandeae tribes. When comparing the chromosome records and the phylogenies of Bignoniaceae it can be deduced that the base number of Bignoniaceae is probably n = 18, followed by an ascendant dysploidy (n = 18 → n = 20) in the most derived and diverse clades. The predominant heterochromatin banding patterns...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome number; CMA/DAPI; Handroanthus; Polyploidy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100303

Genetic variation in growth traits and yield of rubber trees (Hevea brasiliensis) growing in the Brazilian state of São Paulo
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Paulo de Souza; Moraes,Mário Luiz Teixeira de; Bortoletto,Nelson; Costa,Reginaldo Brito da; Gonçalves,Elaine Cristine Pifer.
Analysis of variance and covariance was preformed on growth traits (stem girth, bark thickness, total height gain and rubber yield) of 22 open-pollinated progenies of the rubber tree Hevea brasiliensis from an Asian Hevea collection introduced to Agronomic Institute (Instituto Agronômico, Campinas, São Paulo, Brazil; IAC) in 1952. This progeny trial was replicated at three sites in São Paulo state and it was found that at three years from sowing there was statistically significant variation for girth, bark thickness, height and rubber yield. An individual test sites, values of individual plant heritability for girth ranged from h= 0.36 to h= 0.89 whereas values for heritability for progeny means ranged from h= 0.77 to h= 0.87. These moderate and high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hevea brasiliensis; Rubber tree; Heritability; Genetic parameters.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500019

The quantitative genetic basis of female and male body size and their implications on the evolution of body size dimorphism in the house cricket Acheta domesticus (Gryllidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Castillo,Raúl Cueva del.
Few theoretical and experimental studies have analyzed the genetic basis of body size dimorphism. Since the evolutionary response to selection depends of the genetic variance in a population it is to be expected that traits under selection would have smaller genetic variance than traits not affected by selection. The evolution of sexual size dimorphism is affected by the genetic correlation between females and males, with the most dimorphic traits showing smaller genetic correlations between the sexes. As result of the differences in the intensity of sexual selection between the sexes, it is expected that the levels of genetic variance would be larger in females than males. I analyzed the genetic additive variance underlying six traits of Acheta...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acheta domesticus; Body size; Dimorphism; Heritability; Sexual selection.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500030

Novel splice-affecting variants in CYP27A1 gene in two Chilean patients with Cerebrotendinous Xanthomatosis
Provedor de dados: Genetics and Molecular Biology
Autores: Smalley,Susan V.; Preiss,Yudith; Suazo,José; Vega,Javier Andrés; Angellotti,Isidora; Lagos,Carlos F.; Rivera,Enzo; Kleinsteuber,Karin; Campion,Javier; Martínez,J. Alfredo; Maiz,Alberto; Santos,José Luis.
Cerebrotendinous Xanthomatosis (CTX), a rare lipid storage disorder, is caused by recessive loss-of-function mutations of the 27-sterol hydroxylase (CYP27A1), producing an alteration of the synthesis of bile acids, with an accumulation of cholestanol. Clinical characteristics include juvenile cataracts, diarrhea, tendon xanthomas, cognitive impairment and other neurological manifestations. Early diagnosis is critical, because treatment with chenodeoxycholic acid may prevent neurological damage. We studied the CYP27A1 gene in two Chilean CTX patients by sequencing its nine exons, exon-intron boundaries, and cDNA from peripheral blood mononuclear cells. Patient 1 is a compound heterozygote for the novel substitution c.256-1G &gt; T that causes exon 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebrotendinous Xanthomatosis; Splicing; Mutation; Exon skipping.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100030

Cyanobacterial nitrogenases: phylogenetic diversity, regulation and functional predictions
Provedor de dados: Genetics and Molecular Biology
Autores: Esteves-Ferreira,Alberto A.; Cavalcanti,João Henrique Frota; Vaz,Marcelo Gomes Marçal Vieira; Alvarenga,Luna V.; Nunes-Nesi,Adriano; Araújo,Wagner L.
Abstract Cyanobacteria is a remarkable group of prokaryotic photosynthetic microorganisms, with several genera capable of fixing atmospheric nitrogen (N2) and presenting a wide range of morphologies. Although the nitrogenase complex is not present in all cyanobacterial taxa, it is spread across several cyanobacterial strains. The nitrogenase complex has also a high theoretical potential for biofuel production, since H2 is a by-product produced during N2 fixation. In this review we discuss the significance of a relatively wide variety of cell morphologies and metabolic strategies that allow spatial and temporal separation of N2 fixation from photosynthesis in cyanobacteria. Phylogenetic reconstructions based on 16S rRNA and nifD gene sequences shed light on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyanobacteria; Evolution; Hydrogen; Nitrogen fixation; Molecular phylogeny.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200261

Genome-wide association study of drought-related resistance traits in Aegilops tauschii
Provedor de dados: Genetics and Molecular Biology
Autores: Qin,Peng; Lin,Yu; Hu,Yaodong; Liu,Kun; Mao,Shuangshuang; Li,Zhanyi; Wang,Jirui; Liu,Yaxi; Wei,Yuming; Zheng,Youliang.
Abstract The D-genome progenitor of wheat (Triticum aestivum), Aegilops tauschii, possesses numerous genes for resistance to abiotic stresses, including drought. Therefore, information on the genetic architecture of A. tauschii can aid the development of drought-resistant wheat varieties. Here, we evaluated 13 traits in 373 A. tauschii accessions grown under normal and polyethylene glycol-simulated drought stress conditions and performed a genome-wide association study using 7,185 single nucleotide polymorphism (SNP) markers. We identified 208 and 28 SNPs associated with all traits using the general linear model and mixed linear model, respectively, while both models detected 25 significant SNPs with genome-wide distribution. Public database searches...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aegilops tauschii; Drought resistance; Genome-wide association study; Single nucleotide polymorphism; Wheat.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300398

Metabarcoding reveals that a non-nutritive sweetener and sucrose yield similar gut microbiota patterns in Wistar rats
Provedor de dados: Genetics and Molecular Biology
Autores: Falcon,Tiago; Foletto,Kelly Carraro; Siebert,Marina; Pinto,Denise Entrudo; Andrades,Michael; Bertoluci,Marcello Casaccia.
Abstract The effects of non-nutritive sweeteners (NNS) on the gut microbiota are an area of increasing research interest due to their potential influence on weight gain, insulin resistance, and inflammation. Studies have shown that mice and rats fed saccharin develop weight gain and metabolic alterations, possibly related to changes in gut microbiota. Here, we hypothesized that chronic exposure to a commercial NNS would change the gut microbiota composition in Wistar rats when compared to sucrose exposure. To test this hypothesis, Wistar rats were fed either NNS- or sucrose-supplemented yogurt for 17 weeks alongside standard chow (ad libitum). The gut microbiome was assessed by 16S rDNA deep sequencing. Assembly and quantification were conducted using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 16S rDNA; Deep sequencing; Saccharin; Cyclamate; Yogurt.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100805

Model selection for quantitative trait loci mapping in a full-sib family
Provedor de dados: Genetics and Molecular Biology
Autores: Tong,Chunfa; Zhang,Bo; Li,Huogen; Shi,Jisen.
Statistical methods for mapping quantitative trait loci (QTLs) in full-sib forest trees, in which the number of alleles and linkage phase can vary from locus to locus, are still not well established. Previous studies assumed that the QTL segregation pattern was fixed throughout the genome in a full-sib family, despite the fact that this pattern can vary among regions of the genome. In this paper, we propose a method for selecting the appropriate model for QTL mapping based on the segregation of different types of markers and QTLs in a full-sib family. The QTL segregation patterns were classified into three types: test cross (1:1 segregation), F2 cross (1:2:1 segregation) and full cross (1:1:1:1 segregation). Akaike's information criterion (AIC), the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Full-sib family; Interval mapping; Model selection; Quantitative trait locus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400013

Cytogenetic analyses of two endemic fish species from the São Francisco River basin: Conorhynchus conirostris and Lophiosilurus alexandri (Siluriformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Marques,Marilza Barbosa de Almeida; Moreira-Filho,Orlando; Garcia,Caroline; Margarido,Vladimir Pavan.
Two Siluriformes species endemic to the São Francisco River basin were characterized by conventional and differential cytogenetic analyses involving C-banding, Ag-nucleolar organizer region (NOR) and chromomycin A3 (CMA3) staining, and FISH (fluorescent in situ hybridization) with 18S and 5S rDNA probes. Conorhynchus conirostris presents a higher diploid number (2n = 60) than those detected in Pimelodidae representatives, whereas Lophiosilurus alexandri, with a karyotype of 2n = 54 chromosomes, presents a chromosomal constitution similar to that found in the family Pseudopimelodidae. Plesiomorphic characteristics such as single NORs at terminal positions are found in both species, as revealed by CMA3 and silver nitrate staining, and FISH with a 18S rDNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Incertae sedis; Pimelodidae; Pseudopimelodidae; RDNA; Siluriformes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200008

Chromosomal investigations in patients with mental retardation and/or congenital malformations
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,C.B.; Boy,R.T.; Santos,J.M.; Silva,M.P.S.; Pimentel,M.M.G.
We investigated the chromosomal constitution of patients with mental retardation and/or congenital malformations in order to determine genetic causes for such disturbances. The GTG and CBG banding patterns were studied using phytohemagglutinin M-stimulated lymphocytes cultured from peripheral blood. Among 98 individuals with mental retardation and/or congenital malformations who were analyzed there were 12 cases of Down's syndrome, two of Edward's syndrome, one of Patau's syndrome, five of Turner's syndrome, two of Klinefelter's syndrome, one of "cri-du-chat" syndrome, one case of a balanced translocation between chromosomes 13 and 14, one case of a derivative chromosome and one of a marker chromosome. We found abnormal chromosomes in 26% of the patients,...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400002

A joint effort of the Brazilian Evo-Devo community
Provedor de dados: Genetics and Molecular Biology
Autores: Fonseca,Rodrigo Nunes da; Kohlsdorf,Tiana; Schneider,Igor.
Tipo: Info:eu-repo/semantics/other
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300231

Mucopolysacccharidoses: from understanding to treatment, a century of discoveries
Provedor de dados: Genetics and Molecular Biology
Autores: Giugliani,Roberto.
After the first description of a patient recognized as a MPS case was made in 1917, several similar cases were described and identified. Observations reported in the middle of the twentieth century concerning the presence of acid mucopolysaccharides (later called glycosaminoglycans, or GAGs) in tissues and especially in urine of patients were instrumental in providing an identity for these diseases, which became referred as "mucopolysaccharidoses" (MPS). In the late 1960's it was demonstrated that MPS were caused by defects in the breakdown of GAGs, and the specific enzyme deficiencies for the 11 types and subtypes of MPS were identified thereafter. Genes involved in the MPS were subsequently identified, and a large number of disease-causing mutations were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucopoloysaccharidoses; Lysosomal diseases; Enzyme replacement therapy; Prenatal diagnosis; Newborn screening.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600006

Differentially expressed genes in a flock of Chinese local-breed chickens infected with a subgroup J avian leukosis virus using suppression subtractive hybridization
Provedor de dados: Genetics and Molecular Biology
Autores: Zhao,Guiping; Zheng,Maiqing; Chen,Jilan; Wen,Jie; Wu,Chunmei; Li,Wenjuan; Liu,Libo; Zhang,Yuan.
Avian leukosis virus subgroup J (ALV-J) is a new type of virus that mainly induces myeloid leukosis (ML) in chickens. To further elucidate the pathogenesis of ALV-J infection and tumor development, expression profiles from the bone marrow tissue of 15 infected and 18 non-infected birds from a local-breed poultry-farm under naturally infected conditions, were analyzed by suppression-subtractive hybridization. The birds were diagnosed as ML+ (or ML-) by specific ALV-J detection methods, involving serological tests for antigens and antibodies, and RT-PCR to detect viral RNA. A total of 59 partial gene sequences were revealed by differential screening of 496 forward and 384 reverse subtracted cDNA clones. Of these, 22 identified genes, including 8 up-regulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian leukosis virus subgroup J; Chicken; Transferring gene; MHC genes; Hemoglobin gene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100009

Cytogenetics and DNA barcode reveal an undescribed Apareiodon species (Characiformes: Parodontidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Emanoel Oliveira dos; Deon,Geize Aparecida; Almeida,Rafael Bonfim de; Oliveira,Ezequiel Aguiar de; Nogaroto,Viviane; Silva,Hugmar Pains da; Pavanelli,Carla Simone; Cestari,Marta Margarete; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando; Vicari,Marcelo Ricardo.
Abstract Parodontidae is a small group of fish and some species are particularly difficult to identify due to the lack of sufficiently consistent morphological traits. Cytogenetically, the species possess 2n = 54 chromosomes and are either sex-homomorphic or sex-heteromorphic (regarding its chromosomes). We evaluated data on color, tooth morphology, cytogenetics, and mitochondrial markers (COI) in Apareiodon specimens from the Aripuanã River (Amazon basin) and the results were compared to other congeneric taxa. Morphological results show an overlap of body color and tooth morphology to other known Apareiodon. The cytogenetics data showed that the 2n = 54 chromosomes, 50 m/sm + 4 st and, a ZZ/ZW sex chromosome system in Apareiodon sp. are common to other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal differentiation; Hidden diversity; Repetitive DNA; Sex chromosomes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300365

A molecular method for the detection of sally lightfoot crab larvae (Grapsus grapsus, Brachyura, Grapsidae) in plankton samples
Provedor de dados: Genetics and Molecular Biology
Autores: Ströher,Patrícia R.; Firkowski,Carina R.; Freire,Andrea S.; Pie,Marcio R.
The decapod Grapsus grapsus is commonly found on oceanic islands of the Pacific and Atlantic coasts of the Americas. In this study, a simple, quick and reliable method for detecting its larvae in plankton samples is described, which makes it ideal for large-scale studies of larval dispersal patterns in the species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dispersal; Aratu; Sayapa; Molecular marker.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300026

Spermatogenesis of Zaprionus indianus and Zaprionus sepsoides (Diptera, Drosophilidae): cytochemical, structural and ultrastructural characterization
Provedor de dados: Genetics and Molecular Biology
Autores: Rego,Letícia do Nascimento Andrade de Almeida; Silistino-Souza,Rosana; Azeredo-Oliveira,Maria Tercília Vilela de; Madi-Ravazzi,Lilian.
Zaprionus indianus is a drosophilid native to the Afrotropical region that has colonized South America and exhibits a wide geographical distribution. In contrast, Z. sepsoides is restricted to certain African regions. The two species differ in the size of their testes, which are larger in Z. indianus than in Z. sepsoides. To better understand the biology and the degree of differentiation of these species, the current study evaluated spermatogenesis in males of different ages by conventional staining techniques and ultrastructural analysis. Spermatogenesis and the ultrastructure of spermatozoa were similar in the two species, and the diploid number was confirmed to be 2n = 12. A greater number of spermatozoa were observed in young Z. indianus (1-3 days old)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zaprionus indianus; Zaprionus sepsoides; Spermatogenesis; Cytochemistry; Ultrastructure; Meiosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100008

Genetic characterization of cassava ( Manihot esculenta ) landraces   in Brazil assessed with simple sequence repeats
Provedor de dados: Genetics and Molecular Biology
Autores: Siqueira,Marcos V. B. M.; Queiroz-Silva,Jurema R.; Bressan,Eduardo A.; Borges,Aline; Pereira,Kayo J. C.; Pinto,José G.; Veasey,Elizabeth Ann.
Based on nine microsatellite loci, the aim of this study was to appraise the genetic diversity of 42 cassava ( Manihot esculenta ) landraces from selected regions in Brazil, and examine how this variety is distributed according to origin in several municipalities in the states of Minas Gerais, São Paulo, Mato Grosso do Sul, Amazonas and Mato Grosso. High diversity values were found among the five above-mentioned regions, with 3.3 alleles per locus on an average, a high percentage of polymorphic loci varying from 88.8% to 100%, an average of 0.265 for observed heterozygosity and 0.570 for gene diversity. Most genetic diversity was concentrated within the regions themselves ( H   S   = 0.52). Cluster analysis and principal component based scatter plotting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Microsatellites; SSR markers; Traditional farming.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100015

Complete mitochondrial genome of the lappet moth, Kunugia undans (Lepidoptera: Lasiocampidae): genomic comparisons among macroheteroceran superfamilies
Provedor de dados: Genetics and Molecular Biology
Autores: Kim,Min Jee; Jeong,Jun Seong; Kim,Jong Seok; Jeong,Su Yeon; Kim,Iksoo.
Abstract The mitochondrial genome (mitogenome) characteristics of the monotypic Lasiocampoidea are largely unknown, because only limited number of mitogenomes is available from this superfamily. In this study, we sequenced the complete mitogenome of the lappet moth, Kunugia undans (Lepidoptera: Lasiocampidae) and compared it to those of Lasiocampoidea and macroheteroceran superfamilies (59 species in six superfamilies). The 15,570-bp K. undans genome had one additional trnR that was located between trnA and trnN loci and this feature was unique in Macroheterocera, including Lasiocampoidea. Considering that the two trnR copies are located in tandem with proper secondary structures and identical anticodons, a gene duplication event might be responsible for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kunugia undans; Mitochondrial genome; Lasiocampoidea; Macroheterocera.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400717

Association of CAPN1 and CAST gene polymorphisms with meat tenderness in Bos taurus beef cattle from Argentina
Provedor de dados: Genetics and Molecular Biology
Autores: Corva,Pablo; Soria,Liliana; Schor,Alejandro; Villarreal,Edgardo; Cenci,Macarena Pérez; Motter,Mariana; Mezzadra,Carlos; Melucci,Lilia; Miquel,Cristina; Paván,Enrique; Depetris,Gustavo; Santini,Francisco; Naón,Juan Grigera.
The activity of the calpains/calpastatin proteolytic system is closely related to the postmortem tenderization of meat. We investigated the association between beef tenderness and single nucleotide polymorphism (SNP) markers on the CAPN1 gene (SNP316, alleles C/G; SNP530 alleles A/G) and the CAST gene 3' untranslated region (SNP2870, alleles A/G). We sampled nine slaughter groups comprising 313 steers which had been reared in beef production systems in Argentina between 2002 and 2004 from crosses between Angus, Hereford and Limousin cattle. Minor allele frequencies for the markers were 0.27 to 0.46 (C), 0.02 to 0.18 (A), and 0.24 to 0.53 (A), respectively. The presence of CAPN1 markers had significant effects on meat shear force but no detectable effects...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; CAPN1; CAST; Genetic markers; Meat quality.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600006

Karyotype characterization, constitutive heterochromatin and nucleolus organizer regions of Paranaita opima (Coleoptera, Chrysomelidae, Alticinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Almeida,Mara Cristina de; Campaner,Carlos; Cella,Doralice Maria.
Species of the subtribe Oedionychina not only have a highly uniform diploid number of 2n = 22 (20+X+y) but have the karyotypic peculiarity of possessing extremely large sex chromosomes. We analyzed Paranaita opima embryos and gonadal cells to determine their diploid number, chromosomal morphology, type of sex determination system, constitutive heterochromatin pattern and which chromosomes bear nucleolus organizer regions (NORs). The diploid number of P. opima was 2n = 22 (20+XY/XX) with all chromosomes being metacentric. Chromosome pair 6 showed an interstitial secondary constriction on the short arm. The C-banding technique revealed centromeric constitutive heterochromatin in all chromosomes, which, in pair 6, extended up to the secondary constriction of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C bands; Chromosome; Embryos; Fluorochromes; NOR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300013

Chromosome studies in Turnera (Turneraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Solís Neffa,V.G.; Fernández,A.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400037

CYP1A1, GSTM1, GSTT1 and GSTP1 polymorphisms in an Afro-Brazilian group
Provedor de dados: Genetics and Molecular Biology
Autores: Kvitko,Kátia; Gaspar,Pedro de Abreu; Torres,Martiela Ribeiro; Hutz,Mara H.
Gene polymorphisms involved in the metabolism of drugs and chemical carcinogens seem to be responsible for differences in the susceptibility of individuals to cancer, but genetic population studies are needed to characterize these polymorphisms in different ethnic populations. We investigated polymorphisms of the cytochrome P450 (CYP) gene CYP1A1 and the glutathione S-transferase (GSTs) genes GSTM1, GSTT1 and GSTP1 in a sample of Afro-Brazilians from the southern Brazilian city of Porto Alegre to verify if there were ethnic differences compared to the polymorphisms of the same genes in a previously described sample of Brazilians of European descent from the same city. The allele frequencies detected in the Afro-Brazilian population investigated in this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: African; Brazilian; CYP1A1; European; GSTs; Porto Alegre.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400006

Determination of genetic variability of traditional varieties of Brazilian rice using microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Brondani,Claudio; Borba,Tereza Cristina Oliveira; Rangel,Paulo Hideo Nakano; Brondani,Rosana Pereira Vianello.
The rice (Oryza sativa) breeding program of the Rice and Bean research center of the Brazilian agricultural company Empresa Brasileira de Pesquisa Agropecuária (Embrapa) is well established and provides new cultivars every year to attend the demand for improved high yielding varieties with tolerance to biotic and abiotic stresses. However, the elite genitors used to compose new populations for selection are closely related, contributing to the yield plateau reached in the last 20 years. To overcome this limit, it is necessary to broaden the genetic basis of the cultivars using diverse germplasm such as wild relatives or traditional varieties, with the latter being more practical because they are more easily crossed with elite germplasm to accelerate the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Genetic resources; Microsatellite markers; Oryza sativa.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400017

Clonal stability of tree dryness in Hevea brasiliensis Muell. Arg.
Provedor de dados: Genetics and Molecular Biology
Autores: Omokhafe,K.O.
Clonal stability of tree dryness was evaluated in eleven clones of Hevea brasiliensis at the Rubber Research Institute of Nigeria. The experimental design was the randomized complete block with three replicates and ten trees per replicate. The clones were evaluated in three locations. Four stability parameters were applied. The stability parameters were: environmental variance, regression index, variance due to regression, and Shukla's stability variance. Clone C 202 was outstanding for clonal stability and could be useful for further studies and genetic improvement of tree dryness. Other four clones (C 76, C 150, C 159 and RRIM 600) were also stable.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hevea; Tree dryness; Clones; Stability.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200019

Phenotypic and behavioral variability within Angelman Syndrome group with UPD
Provedor de dados: Genetics and Molecular Biology
Autores: Fridman,Cintia; Varela,Monica C.; Valente,Kette; Marques-Dias,Maria J.; Koiffmann,Célia P.
The Angelman syndrome (AS) (developmental delay, mental retardation, speech impairment, ataxia, outbursts of laughter, seizures) can result either from a 15q11-q13 deletion, or from paternal uniparental disomy (UPD), imprinting, or UBE3A mutations. We describe here the phenotypic and behavioral variability detected in eight UPD patients out of a group of 58 AS patients studied. All of them presented developmental delay, mental retardation, ataxia, speech impairment, and frequent drooling. Only one had microcephaly, whereas in two of them the OFC (head circumference) was above the 98th percentile. The weight of all patients was above the 50th percentile, and in three of them the height was above the 90th percentile. Three were able to say a few words and to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angelman syndrome; Uniparental disomy; Macrosomy; Macrocephaly.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200002

A study of the distribution of phylogenetically conserved blocks within clusters of mammalian homeobox genes
Provedor de dados: Genetics and Molecular Biology
Autores: Stojanovic,Nikola.
Genome sequencing efforts of the last decade have produced a large amount of data, which has enabled whole-genome comparative analyses in order to locate potentially functional elements and study the overall patterns of phylogenetic conservation. In this paper we present a statistically based method for the characterization of these patterns in mammalian DNA sequences. We have applied this approach to the study of exceptionally well conserved homeobox gene clusters (Hox), based on an alignment of six species, and we have constructed a map of Hox cataloguing the conserved fragments, along with their locations in relation to the genes and other landmarks, sometimes showing unexpected layouts.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genomics; Phylogenetic conservation; Multiple sequence alignments; Heaviest segments; Hox.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300034

Fine mapping and single nucleotide polymorphism effects estimation on pig chromosomes 1, 4, 7, 8, 17 and X
Provedor de dados: Genetics and Molecular Biology
Autores: Hidalgo,André M.; Lopes,Paulo S.; Paixão,Débora M.; Silva,Fabyano F.; Bastiaansen,John W.M.; Paiva,Samuel R.; Faria,Danielle A.; Guimarães,Simone E.F.
Fine mapping of quantitative trait loci (QTL) from previous linkage studies was performed on pig chromosomes 1, 4, 7, 8, 17, and X which were known to harbor QTL. Traits were divided into: growth performance, carcass, internal organs, cut yields, and meat quality. Fifty families were used of a F2 population produced by crossing local Brazilian Piau boars with commercial sows. The linkage map consisted of 237 SNP and 37 microsatellite markers covering 866 centimorgans. QTL were identified by regression interval mapping using GridQTL. Individual marker effects were estimated by Bayesian LASSO regression using R. In total, 32 QTL affecting the evaluated traits were detected along the chromosomes studied. Seven of the QTL were known from previous studies using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bayesian LASSO Piau breed pig genetics; QTL.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400009

Genetics of in vitro organogenesis and precocious germination of wheat embryos
Provedor de dados: Genetics and Molecular Biology
Autores: Lange,Claudia E.; Federizzi,Luiz C.; Carvalho,Fernando I.F.; Dornelles,Ana L.C.; Handel,Cristine L.
The genetic bases of in vitro organogenesis and precocious germination of embryos in immature wheat embryo culture were investigated using six Brazilian genotypes and their F1, F2, BC1F1 and BC2F1 generations in a generation means analysis. Four parents and one set of F1’s were also analyzed in a diallel experiment. The results indicated a complex gene action controlling both traits, with additive, dominant and epistatic effects. High broad sense heritability values were found, indicating genetic determination. Considering the complexity of gene control, genetic gain could be achieved by selecting for the traits in advanced generations of the segregating population. Low correlation values between organogenesis, precocious germination, regeneration and...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100015

Genetic parameters of test day milk yields of Holstein cows
Provedor de dados: Genetics and Molecular Biology
Autores: Machado,S.G.; Freitas,M.A.R.; Gadini,C.H.
Data were obtained from 17,968 records from 2,130 first lactations of Holstein cows calving between 1988 and 1991. The subjects were daughters of 136 sires monitored by Brazilian Breeders Association, Animal Science Institute, Department of Agriculture, a branch of the State of São Paulo. Data were divided into 10 subsets based on the number of days in milk yield. Test day milk yields (M1 to M10) and 305-day milk yield (M305) were the traits studied. These traits were adjusted for several environmental effects: class of cow age at calving, interval from calving to first test day, and herd-year-season. Restricted maximum likelihood estimates of (co)variance components were obtained from one and two-traits analysis under a sire model. Estimates of...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300016

Boron transport in Eucalyptus. 2. Identification in silico of a putative boron transporter for xylem loading in eucalypt
Provedor de dados: Genetics and Molecular Biology
Autores: Domingues,Douglas Silva; Leite,Susi Meire Maximino; Farro,Ana Paula Cazerta; Coscrato,Virgínia Elias; Mori,Edson Seizo; Furtado,Edson Luiz; Wilcken,Carlos Frederico; Velini,Edivaldo Domingues; Guerrini,Iraë Amaral; Maia,Ivan Godoy; Marino,Celso Luis.
Boron (B) is a low mobility plant micronutrient whose molecular mechanisms of absorption and translocation are still controversial. Many factors are involved in tolerance to Boron excess or deficiency. Recently, the first protein linked to boron transport in biological systems, BOR1, was characterized in Arabidopsis thaliana. This protein is involved in boron xylem loading and is similar to bicarbonate transporters found in animals. There are indications that BOR1 is a member of a conserved protein family in plants. In this work, FORESTS database was used to identify sequences similar to this protein family, looking for a probable BOR1 homolog in eucalypt. We found five consensus sequences similar to BOR1; three of them were then used in multiple alignment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus; Boron; Xylem loading; Expressed Sequence Tag (EST); BOR1.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400017

Morphological dimorphism in the Y chromosome of "pé-duro" cattle in the Brazilian State of Piauí
Provedor de dados: Genetics and Molecular Biology
Autores: Britto,Carmen M.C.; Mello,Maria Luiza S.
"Pé-duro" (hard foot) is a rare breed of beef cattle of European (Bos taurus taurus) origin, originated in northern and northeastern Brazil. Y chromosome morphology, outer genital elements and other phenotypic characteristics were examined in 75 "pé-duro" bulls from the Empresa Brasileira de Pesquisa Agropecuária (Embrapa) herd in the Brazilian State of Piauí. The purpose was to investigate possible racial contamination with Zebu animals (Bos taurus indicus) in a cattle that has been considered closest to its European origin (B. t. taurus). The presence of both submetacentric and acrocentric Y chromosomes, typical of B. t. taurus and B. t. indicus, respectively, and the larger preputial sheath in bulls with an acrocentric Y chromosome indicated racial...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300014

Clonal chromosome abnormalites found in three non-neoplastic proliferative brain lesions
Provedor de dados: Genetics and Molecular Biology
Autores: Rainho,Cláudia Aparecida; Barbieri Neto,José; Moraes,Lamartine Correa de; Rogatto,Silvia Regina.
Chromosome analysis was made of brain lesions from three patients which, according to classical histopathological criteria, did not contain tumor cells. In addition to normal cells, we identified abnormal karyotypes with clonal numerical and structural chromosome alterations in at least two independently originated primary cultures from each lesion. Our data suggest that chromosomal aberrations can exist in vivo in non-neoplastic lesions. Other abnormalities may be due to genetic instability manifested only in vitro (culture artifacts) or may already have been present in brain tissue, reflecting previous chromosome damage (as a result of exposure to chemical treatment or enviromental clastogens).
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100006

cDNA-AFLP analysis of gene expression differences between the flower bud and sprout-shoot apical meristem of Angelica sinensis (Oliv.) Diels
Provedor de dados: Genetics and Molecular Biology
Autores: Guang,Yu; Jinao,Duan; Hui,Yan; Bingsheng,Song; Ziqing,He.
Angelica sinensis (Oliv.) Diels (Umbelliferae) is a well-known medicinal plant mainly distributed in Gansu Province of China. Its local and global demand is significant because of its food and medicinal applications. However, the early bolting rate of Angelica sinensis (Oliv.) Diels reaches 20%-60%, which seriously affects its food and medicinal qualities. Thus, differences in gene expression between the flower bud and sprout-shoot apical meristem underwent analysis, by means of cDNA-amplified restriction fragment length polymorphism, to better understand the flowering mechanism. 64 primer sets, each of which amplified to 60 transcript-derived fragments (TDFs), were used. Among these TDFs, 26 were expressed specifically in the flower bud. After cloning and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CDNA-AFLP; Early bolting; Angelica sinensis (Oliv.) Diels; Gene expression; TDFs.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200018

Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
Provedor de dados: Genetics and Molecular Biology
Autores: Liang,Qiaoyi; Ding,Jiayi; Zheng,Shu.
Up-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1.3-kb RNA consisting of four exons is transcribed from an Alu element upstream of a 5.0-kb structurally incomplete HERV-H element. RT-PCR and quantitative RT-PCR results indicated that expression of this HERV-related transcript was negatively associated with colon, stomach, and kidney cancers. Its expression was induced upon treatment with DNA methylation and histone deacetylation inhibitors. A BLAT search using long terminal repeats (LTRs)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endogenous retrovirus; Noncoding RNA; RT-PCR; Quantitative real-time PCR; Transcription.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005

Multiple interstitial ribosomal sites (NORs) in the Brazilian squirrel Sciurus aestuans ingrami (Rodentia, Sciuridae) with 2n = 40: an overview of Sciurus cytogenetics
Provedor de dados: Genetics and Molecular Biology
Autores: Fagundes,Valéria; Christoff,Alexandre Uarth; Amaro-Ghilard,Renata Cecília; Scheibler,Daniel R.; Yonenaga-Yassuda,Yatiyo.
This is the first time the karyotype of the Brazilian squirrel Sciurusaestuans ingrami, with 2n = 40, is described. The karyotype of this species comprises 18 pairs of biarmed and one minute pair of acrocentric autosomes, a medium-sized submetacentric X and a medium-sized acrocentric Y. Four pairs have an interstitial secondary constriction, co-located with nucleolar organizer regions (NORs), identified by silver-staining technique and fluorescent in situ hybridization (FISH) with ribosomal 18S/28S probes. The occurrence of multiple interstitial NORs is rare in rodents, and this is one of the few examples, identified by a molecular cytogenetics approach.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Squirrels; Chromosomes; Karyotype; NORs; Rodentia.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300007

Theory and analysis of partial diallel crosses
Provedor de dados: Genetics and Molecular Biology
Autores: Viana,José Marcelo Soriano; Cruz,Cosme Damião; Cardoso,Antonio Américo.
This study presents theory and analysis of partial diallel crosses based on Hayman's methods. This genetic design consists of crosses among two parental groups. It should be used when there are two groups of parents, for example, dent and flint maize inbred lines, and the breeder is not interested in the assessment of crosses between parents of the same group. Analyses are carried out using data from the parents and their F1 hybrids allowing a detailed characterization of the polygenic systems under study and the choice of parents for hybridization. Diallel analysis allows the estimation of genetic and non-genetic components of variation and genetic parameters and to assess the following: genetic variability in each group; genotypic differences between...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400021

Genetic characterization of Uruguayan Pampa Rocha pigs with microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Montenegro,M; Llambí,S; Castro,G; Barlocco,N; Vadell,A; Landi,V; Delgado,JV; Martínez,A.
In this study, we genetically characterized the Uruguayan pig breed Pampa Rocha. Genetic variability was assessed by analyzing a panel of 25 microsatellite markers from a sample of 39 individuals. Pampa Rocha pigs showed high genetic variability with observed and expected heterozygosities of 0.583 and 0.603, respectively. The mean number of alleles was 5.72. Twenty-four markers were polymorphic, with 95.8% of them in Hardy Weinberg equilibrium. The level of endogamy was low (FIS = 0.0475). A factorial analysis of correspondence was used to assess the genetic differences between Pampa Rocha and other pig breeds; genetic distances were calculated, and a tree was designed to reflect the distance matrix. Individuals were also allocated into clusters. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic resources; Microsatellites.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100048

Mutational analysis of xenobiotic metabolizing genes (CYP1A1 and GSTP1) in sporadic head and neck cancer patients
Provedor de dados: Genetics and Molecular Biology
Autores: Masood,Nosheen; Kayani,Mahmood Akhtar.
CYP1A1 is the phase I enzyme that detoxifies the carcinogen or converts it into a more electrophilic form, metabolized by phase II enzymes like GSTP1. These detoxifying genes have been extensively studied in association with head and neck cancer (HNC) in different ethnic groups worldwide. The current study was aimed at screening genetic polymorphisms of genes CYP1A1 and GSTP1 in 388 Pakistani HNC patients and 150 cancer-free healthy controls, using PCR-SSCP. No already known variants of either gene were found, however a novel frameshift mutation due to insertion of T (g.2842_2843insT) was observed in the CYP1A1 gene. A statistically significant number (5.4%) of HNC cases, with the mean age of 51.75 (±15.7) years, presented this frameshift mutation in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GSTP1; CYP1A1; Head and neck cancer; Polymorphisms; Mutations.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400001

Evolutionary relationships in the genus Zea: analysis of repetitive sequences used as cytological FISH and GISH markers
Provedor de dados: Genetics and Molecular Biology
Autores: Poggio,Lidia; Confalonieri,Viviana; Comas,Cecilia; Gonzalez,Graciela; Naranjo,Carlos A.
The present study is a revision of our work on evolutionary cytogenetics of the genus Zea, including several new experiments which give a deeper insight into the nature of the DNA sequences involved in telomeric regions of Zea luxurians. These new experiments, based on the Southern blotting technique and in situ hybridization, have demonstrated the following: 1) in situ hybridization (FISH) demonstrated the presence of the 180-bp repeat maize-knob-repeat-sequence in DAPI-positive terminal heterochromatic blocks of Z. luxurians (ZL-THB region); 2) Southern blot analysis confirmed that the 180-bp repeat present in maize is also present in Z. diploperennis, Z. luxurians and Tripsacum dactyloides, but not in Z. perennis; 3) another sequence with targeted sites...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400048

Mitochondrial DNA D-loop sequence variation among 5 maternal lines of the Zemaitukai horse breed
Provedor de dados: Genetics and Molecular Biology
Autores: Cothran,E. Gus; Juras,Rytis; Macijauskiene,Vale.
Genetic variation in Zemaitukai horses was investigated using mitochondrial DNA (mtDNA) sequencing. The study was performed on 421 bp of the mitochondrial DNA control region, which is known to be more variable than other sections of the mitochondrial genome. Samples from each of the remaining maternal family lines of Zemaitukai horses and three random samples for other Lithuanian (Lithuanian Heavy Draught, Zemaitukai large type) and ten European horse breeds were sequenced. Five distinct haplotypes were obtained for the five Zemaitukai maternal families supporting the pedigree data. The minimal difference between two different sequence haplotypes was 6 and the maximal 11 nucleotides in Zemaitukai horse breed. A total of 20 nucleotide differences compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: D-loop; Equus caballus; Phylogeny; Polymorphism.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500006

Barber-Say syndrome: further delineation of the clinical spectrum
Provedor de dados: Genetics and Molecular Biology
Autores: Cortés,Fanny M.; Troncoso,Ledia A.; Alliende,Angélica R.; Curotto,Bianca L.
We report on a 14-year-old girl who presented a multiple congenital anomaly pattern: ablepharon, hypertelorism, telecanthus, macrostomia, helix agenesis of both ears, redundant thick skin and severe hirsutism, the 5th reported case of Barber-Say syndrome. Our patient had almost the same phenotype as that of the patient cited by Martínez Santana et al. (Am. J. Med. Genet. 47: 20-23, 1993) including the same until then undescribed dermatoglyphic pattern.
Tipo: Info:eu-repo/semantics/report
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200003

Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunits
Provedor de dados: Genetics and Molecular Biology
Autores: Sammour,Reda H.
The genes encoding the glycinin subunits G2 and G4 were molecularly manipulated and modified to test the possibility of increasing the nutritional value of soybean seed proteins. The recombinant DNAs pSP65/G2HG4, pSP65/G4HG2, pSP65/248 Metl, pSP65/248 Met2,3 and pSP65/248 Metl.2,3 were used in in vitro translation to produce (i) chimeric proteins consisting of reciprocally exchanged acidic and basic G2 and G4 domains and (ii) Gy4 point mutants with an increased number of methionine residues. The ability of the recombinant proteins to assemble into proper quaternary structures was investigated using sucrose gradient fractionation. The data produced by this study could provide valuable clues for the potential improvement of genetically modified crops.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CDNA; Glycinin subunit G4; In vitro translation; Mutagenesis; Soybean.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300025

Comparative cytogenetics among species of the Astyanax scabripinnis complex: evolutionary and biogeographical inferences
Provedor de dados: Genetics and Molecular Biology
Autores: Vicari,Marcelo Ricardo; Noleto,Rafael Bueno; Artoni,Roberto Ferreira; Moreira-Filho,Orlando; Bertollo,Luiz Antonio Carlos.
Karyotype data are presented for distinct species of the genus Astyanax from four rivers belonging to three different hydrographic basins of the State of Paraná, Brazil (Verde River - Tibagi basin, Açungui River - Ribeira basin, and Santo Antônio and Jaguariaíva Rivers - Jaguariaíva basin). Three karyotypic forms were identified, here denominated karyotype A (2n = 50 chromosomes, with 8m+18sm+10st+14a, and thirteen 18S rDNA sites); karyotype B (2n = 50 chromosomes, with 8m+18sm+10st+1f4a, and four 18S rDNA sites); and karyotype C (2n = 48 chromosomes, with 10m+16sm+10st+12a, and eight 18S rDNA sites). The pattern of constitutive heterochromatin was similar among the three karyotypic forms, with few differences. The 5S rDNA corresponds to a synapomorphic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype evolution; Cytotaxonomy; RDNA sites; Biogeography.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200002

Gene projects: a genome web tool for ongoing mining and annotation applied to CitEST
Provedor de dados: Genetics and Molecular Biology
Autores: Carazzolle,Marcelo F.; Formighieri,Eduardo F.; Digiampietri,Luciano A.; Araujo,Marcos R.R.; Costa,Gustavo G.L.; Pereira,Gonçalo A.G.
Genome projects, both genomic DNA and ESTs (cDNA), generate a large amount of information, demanding time and a well-structured bioinformatics laboratory to manage these data. These genome projects use information available in heterogeneous formats from different sources. The amount and heterogeneity of this information, as well as the absence of a world consensus pattern, make the integration of these data a difficult task. At the same time, sub-tasks, such as microarray analyses of these projects, are very complex. This creates a demand for the development of creative solutions for ongoing annotation, thematic projects, microarray experiments, etc. This paper presents Gene Projects, a system developed to integrate all kinds of solutions.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Annotation; Genome; Web; Microarray and clustering.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500032

Whole exome sequencing identifies a novel variant in an apoptosis-inducing factor gene associated with X-linked recessive hearing loss in a Chinese family
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,Qi; Xingxing,Lu; Ding,Zhiwei; Qi,Yu; Liu,Yuhe.
Abstract We report on the genetic analysis of a Chinese family in which four male patients presented with postlingual progressive hearing loss, associated with distal muscle wasting and unsteady ataxic gait. Using whole exome sequencing, we identified a new pathogenic variant (c.1463C&gt;T, p.Pro488Leu) in the AIFM1 gene, which encodes the apoptosis-inducing factor mitochondrion-associated 1 precursor. AIFM1 is involved in the mitochondrial respiratory chain and cellular caspase-independent apoptosis pathway and has been reported to cause multiple phenotypes including hearing loss. The p.Pro488Leu missense variant segregated with symptoms in the pedigree. It was not found in the dbSNP database, databases of genomes and SNPs in the Chinese population, in 74...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Whole exome sequencing; Apoptosis-inducing factor; AIFM1; X-linked recessive hereditary hearing loss.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400543

Relationship between mitochondrial gene rearrangements and stability of the origin of light strand replication
Provedor de dados: Genetics and Molecular Biology
Autores: Fonseca,Miguel M.; Harris,D. James.
Mitochondrial gene rearrangements are much more frequent in vertebrates than initially thought. It has been suggested that the origin of light strand replication could have an important role in the process of gene rearrangements, but this hypothesis has never been tested before. We used amphibians to test the correlation between light-strand replication origin thermodynamic stability and the occurrence of gene rearrangements. The two variables were correlated in a non-phylogenetic approach, but when tested in a phylogenetically based comparative method the correlation was not significant, although species with unstable light-strand replication origins were much more likely to have undergone gene rearrangements. This indicates that within amphibians there...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA replication; Gene rearrangements; Origin of replication; Secondary structure; Amphibians.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300027

Karyotype differentiation of four Cestrum species (Solanaceae) revealed by fluorescent chromosome banding and FISH
Provedor de dados: Genetics and Molecular Biology
Autores: Fernandes,Thiago; Rego,Letícia do Nascimento Andrade de Almeida; Nardy,Mariana; Yuyama,Priscila Mary; Vanzela,André Luís Laforga.
The karyotypes of four South American species of Cestrum (C. capsulare, C. corymbosum, C. laevigatum and C. megalophylum) were studied using conventional staining, C-CMA/DAPI chromosome banding and FISH with 45S and 5S rDNA probes. The karyotypes showed a chromosome number of 2n = 2x = 16, with metacentric chromosomes, except for the eighth submeta- to acrocentric pair. Several types of heterochromatin were detected, which varied in size, number, distribution and base composition. The C-CMA+ bands and 45S rDNA were located predominantly in terminal regions. The C-CMA+/DAPI+ bands appeared in interstitial and terminal regions, and the C-DAPI+ bands were found in all chromosome regions. The 5S rDNA sites were observed on the long arm of pair 8 in all species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cestrum; Chromosome banding; FISH; Heterochromatin; Physical maps; 45S and 5S rDNA.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200019

Phylogenetic relationships among Capuchin (Cebidae, Platyrrhini) lineages: An old event of sympatry explains the current distribution of Cebus and Sapajus
Provedor de dados: Genetics and Molecular Biology
Autores: Martins-Junior,Antonio Marcio Gomes; Carneiro,Jeferson; Sampaio,Iracilda; Ferrari,Stephen F.; Schneider,Horacio.
Abstract Capuchin monkeys are currently represented by four species of Cebus and eight of Sapajus. This group is taxonomically complex and several questions still need to be clarified. In the current study, using mtDNA markers and a larger sample representation than in previous studies, we seek to understand the phylogenetic relationships among the capuchin lineages and their historical biogeography. All 12 species of capuchins were analyzed for the mitochondrial Control Region and Cytochrome b to test two biogeographical hypotheses: “Reinvasion of the Amazon (ROA)” and “Sympatric Evolution (SEV)”. The phylogenetic relationships among distinct lineages within genera is consistent with an evolutionary diversification pattern probably resulting from an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capuchins; Phylogeography; Phylogeny; Taxonomy; Biogeography.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400699

In silico prediction of gene expression patterns in Citrus flavedo
Provedor de dados: Genetics and Molecular Biology
Autores: Berger,Irving J.; Freitas-Astúa,Juliana; Reis,Marcelo S.; Targon,Maria Luísa P.N.; Machado,Marcos A.
Out of the 18,942 flavedo expressed sequences (clusters plus singletons) in Citrus sinensis from the Citrus EST Project (CitEST), 25 were statistically supported to be differentially expressed in this tissue after a double in silico hybridization strategy against leaf-, flower-, and bark-derived ESTs. Five of them, two terpene synthases and three O-methyltransferases, are absent in the other citrus tissues with concomitant 2x2 statistics, supporting the hypothesis that they are putative flavedo-specific expressed sequences. The pattern of these differentially expressed sequences during fruit development suggests that most of them are developmentally regulated. Some expressed gene products, including a putative germin-like protein highly expressed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EST; Fruit; Gene expression; Orange; Tissue-specific.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500004

Karyotype morphology and evolution in some Lathyrus (Fabaceae) species of southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Klamt,Adriane; Schifino-Wittmann,Maria Teresa.
The karyotypes of Lathyrus nervosus Lam., L. pubescens Hook. et Arn., L. paranensis Burk. and L. crassipes Gill ap. Hook et Arn., native to Rio Grande do Sul (southern Brazil), are described in detail for the first time. All taxa have 2n = 14 chromosomes. The karyotypic formulae were 2 m + 12 sm for L. nervosus, L. pubescens and L. paranensis and 4 m + 10 sm for L. crassipes. In all species, the smallest chromosome pair bore a secondary constriction with a satellite in the long arm. Intraspecific variability in the position and number of secondary constrictions was observed in L. nervosus and L. pubescens. All of the species had a conservative and similar karyotype morphology, but differed in total complement size by as much as 20% between the highest (L....
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200036

Gene expression profile analysis of human intervertebral disc degeneration
Provedor de dados: Genetics and Molecular Biology
Autores: Chen,Kai; Wu,Dajiang; Zhu,Xiaodong; Ni,Haijian; Wei,Xianzhao; Mao,Ningfang; Xie,Yang; Niu,Yunfei; Li,Ming.
In this study, we used microarray analysis to investigate the biogenesis and progression of intervertebral disc degeneration. The gene expression profiles of 37 disc tissue samples obtained from patients with herniated discs and degenerative disc disease collected by the National Cancer Institute Cooperative Tissue Network were analyzed. Differentially expressed genes between more and less degenerated discs were identified by significant analysis of microarray. A total of 555 genes were significantly overexpressed in more degenerated discs with a false discovery rate of &lt; 3%. Functional annotation showed that these genes were significantly associated with membrane-bound vesicles, calcium ion binding and extracellular matrix. Protein-protein interaction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genes; Intervertebral disc degeneration; Molecular classification; Protein-protein interaction.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300021

Hypomethylation of cytosine residues in cold-sensitive regions of Cestrum strigilatum (Solanaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Guarido,Paula Carolina Paes; Paula,Adriano Alves de; Silva,Carlos Roberto Maximiano da; Rodriguez,Carmen; Vanzela,André Luís Laforga.
Heterochromatin comprises a fraction of the genome usually with highly repeated DNA sequences and lacks of functional genes. This region can be revealed by using Giemsa C-banding, fluorochrome staining and cytomolecular tools. Some plant species are of particular interest through having a special type of heterochromatin denominated the cold-sensitive region (CSR). Independent of other chromosomal regions, when biological materials are subjected to low temperatures (about 0 °C), CSRs appear slightly stained and decondensed. In this study, we used Cestrum strigilatum (Solanaceae) to understand some aspects of CSR condensation associated with cytosine methylation levels, and to compare the behavior of different heterochromatin types of this species, when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-5-methylcytosine; Cestrum; Chromosomes; CMA/DAPI banding; Heterochromatin; Immunolabeling.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300012

Predisposition to cancer and radiosensitivity
Provedor de dados: Genetics and Molecular Biology
Autores: Pichierri,P.; Franchitto,A.; Palitti,F.
Many cancer-prone diseases have been shown to be radiosensitive. The radiosensitivity has been attributed to pitfalls in the mechanisms of repair of induced DNA lesions or to an impaired cell cycle checkpoint response. Although discrepancies exist in the results obtained by various authors on the radiosensitivity of individuals affected by the same disease, these can be attributed to the large variability observed already in the response to radiation of normal individuals. To date three test are commonly used to assess radiosensitivity in human cells: survival, micronucleous and G2 chromosomal assay. The three tests may be performed using either fibroblasts or peripheral blood lymphocytes and all the three tests share large interindividual variability. In...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400058

Variants of the HNF1α gene: a molecular approach concerning diabetic patients from southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Bonatto,Naieli; Nogaroto,Viviane; Svidnicki,Paulo V.; Milléo,Fábio Q.; Grassiolli,Sabrina; Almeida,Mara C.; Vicari,Marcelo R.; Artoni,Roberto F.
Maturity Onset Diabetes of the Young (MODY) presents monogenic inheritance and mutation factors which have already been identified in six different genes. Given the wide molecular variation present in the hepatocyte nuclear factor-1α gene (HNF1α) MODY3, the aimof this study was to amplify and sequence the coding regions of this gene in seven patients from the Campos Gerais region, Paraná State, Brazil, presenting clinical MODY3 features. Besides the synonymous variations, A15A, L17L, Q141Q, G288G and T515T, two missense mutations, I27L and A98V, were also detected. Clinical and laboratory data obtained from patients were compared with the molecular findings, including the I27L polymorphism that was revealed in some overweight/obese diabetic patients of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MODY3; Molecular diagnosis; Diabetes mellitus; Nucleotide sequencing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500005

Six1 induces protein synthesis signaling expression in duck myoblasts mainly via up-regulation of mTOR
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,Haohan; Li,Xinxin; Liu,Hehe; Sun,Lingli; Zhang,Rongping; Li,Liang; Wangding,Mincheng; Wang,Jiwen.
Abstract As a critical transcription factor, Six1 plays an important role in the regulation of myogenesis and muscle development. However, little is known about its regulatory mechanism associated with muscular protein synthesis. The objective of this study was to investigate the effects of overexpression ofSix1 on the expression of key protein metabolism-related genes in duck myoblasts. Through an experimental model where duck myoblasts were transfected with a pEGFP-duSix1 construct, we found that overexpression of duckSix1 could enhance cell proliferation activity and increase mRNA expression levels of key genes involved in the PI3K/Akt/mTOR signaling pathway, while the expression of FOXO1, MuRF1and MAFbx was not significantly altered, indicating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Duck; Six1; Protein metabolism signaling; MTOR; PEGFP-duSix1.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100151

Maintenance of syntenic groups between Cathartidae and Gallus gallus indicates symplesiomorphic karyotypes in new world vultures
Provedor de dados: Genetics and Molecular Biology
Autores: Tagliarini,Marcella M.; O'Brien,Patricia C.M.; Ferguson-Smith,Malcolm A.; Oliveira,Edivaldo H.C. de.
Similarities between New World and Old World vultures have been interpreted to reflect a close relationship and to suggest the inclusion of both in Accipitridae (Falconiformes). However, deeper analyses indicated that the placement of the New World vultures (cathartids) in this Order is uncertain. Chromosome analysis has shown that cathartids retained a karyotype similar to the putative avian ancestor. In order to verify the occurrence of intrachromosomal rearrangements in cathartids, we hybridized whole chromosome probes of two species (Gallus gallus and Leucopternis albicollis) onto metaphases of Cathartes aura. The results showed that not only were the syntenic groups conserved between Gallus and C. aura, but probably also the general gene order,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cathartes; FISH; Gallus; Leucopternis; Whole-chromosome probes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100015

Ancestral association between HLA and HFE H63D and C282Y gene mutations from northwest Colombia
Provedor de dados: Genetics and Molecular Biology
Autores: Rodriguez,Libia M; Giraldo,Mabel C; Velasquez,Laura I; Alvarez,Cristiam M; Garcia,Luis F; Jimenez-Del-Rio,Marlene; Velez-Pardo,Carlos.
A significant association between HFE gene mutations and the HLA-A*03-B*07 and HLA-A*29-B*44 haplotypes has been reported in the Spanish population. It has been proposed that these mutations are probably connected with Celtic and North African ancestry, respectively. We aimed to find the possible ancestral association between HLA alleles and haplotypes associated with the HFE gene (C282Y and H63D) mutations in 214 subjects from Antioquia, Colombia. These were 18 individuals with presumed hereditary hemochromatosis (“HH”) and 196 controls. The HLA-B*07 allele was in linkage disequilibrium (LD) with C282Y, while HLA-A*23, A*29, HLA-B*44, and B*49 were in LD with H63D. Altogether, our results show that, although the H63D mutation is more common in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hereditary hemochromatosis; HLA class I genes; HFE gene; H63D; C282Y.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100008

Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology
Provedor de dados: Genetics and Molecular Biology
Autores: Cassel,Ana Paula Rebelo; Barcellos,Regina Bones; Silva,Cláudia Maria Dornelles da; Almeida,Sabrina Esteves de Matos; Rossetti,Maria Lucia Rosa.
The aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of high-risk types 16, 18, 31, 33, 39, 45 and 59 were also investigated. Of the 158 samples, 20 (12.7%) and 47 (29.7%) were positive for HPV-DNA and MN, respectively. Evidence for MN was found in 11 out of 20 (55%) HPV-DNA positive samples and in 36 out of 138 (26.1%) HPV-DNA negative ones. MN presence was significantly higher in HPV-DNA positive samples (p = 0.016). On the other hand, the absence of MN observed in a considerable number of HPV-DNA negative samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Micronucleus; Cytology; High-risk HPV; Southern Brazil.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300007

Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation
Provedor de dados: Genetics and Molecular Biology
Autores: Mittmann,Josane; Kerkis,Irina; Kawashima,Cinthia; Sukoyan,Marina; Santos,Enrico; Kerkis,Alexandre.
We studied the karyotypes of three hybrid clones of mouse embryonic stem cells and murine splenocytes (two having near diploid and one having near tetraploid chromosome numbers) and the characteristics of their differentiation during the formation of embryoid bodies. The X chromosome originating from embryonic stem cells may be lost in hybrids with a near diploid chromosome number and reprogramming of the "somatic" X may occur. The morphological data we obtained using light and electron microscopy revealed a correlation between the karyotype constitution of hybrid cells and their differentiation during the formation of embryoid bodies. At the beginning of development, the embryoid bodies derived from hybrid cells already showed an advanced degree of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mouse ES-somatic cell hybrids; Karyotype; Pluriopotency; Differentiation; Embryoid bodies.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100019

Late-replicating X-chromosome: replication patterns in mammalian females
Provedor de dados: Genetics and Molecular Biology
Autores: Tunin,Karen; Otto,Priscila Guimarães.
The GTG-banding and 5-BrdU incorporation patterns of the late-replicating X-chromosome were studied in female dogs and cattle, and compared to human female patterns. The replication patterns of the short arm of the X-chromosomes did not show any difference between human, dog and cattle females. As to the long arm, some bands showed differences among the three studied species regarding the replication kinetics pattern. These differences were observed in a restricted region of the X-chromosome, delimited by Xq11 -&gt; q25 in humans, by Xq1 -&gt; q8 in dogs, and by Xq12 -&gt; q32 in cattle. In an attempt to find out if these differences in the replication kinetics could be a reflection of differences in the localization of genes in that region of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: X-chromosome; Replication patterns; Mammalian females.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300009

Physical mapping of the 18S and 5S ribosomal genes in nine Characidae species (Teleostei, Characiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Peres,Wellington Adriano Moreira; Bertollo,Luiz Antonio Carlos; Moreira Filho,Orlando.
Characidae is one of the largest fish families of the Neotropical region, and presenting a pronounced morphological variability, certainly does not constitute a monophyletic group. The cytogenetical data also show a large chromosomal variation and can provide important information for a better understanding of the relationships between the species of this group. 18S and 5S rDNA probes were used in the present study for the chromosomal mapping in different Characidae species from the São Francisco River (Astyanax lacustris, Astyanax scabripinnis, Hasemania nana, Piabina argentea, Orthospinus franciscensis, Serrapinnus heterodon, Serrapinnus piaba and Myleus micans) and Alto Paraná (Astyanax altiparanae) basins. Species with a single pair of chromosomes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Neotropical fish; Fluorescent in situ hybridization (FISH); RDNA probe.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200009

Cytotaxonomic study of the Chilean endemic complex Alstroemeria magnifica Herb. (Alstroemeriaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Baeza,Carlos M.; Finot,Víctor; Ruiz,Eduardo; Carrasco,Pedro; Novoa,Patricio; Rosas,Marcelo; Toro-Núñez,Oscar.
Abstract Alstroemeria L. (Alstroemeriaceae) represents one of the most diverse genera of vascular plants in Chile. It contains approximately 54 taxa, 40 of which are endemic. The “complex” Alstroemeria magnifica is endemic to Chile, and it comprises four varieties: A. magnifica var. magenta, A. magnifica var. magnifica, A. magnifica var. sierrae, and A. magnifica var. tofoensis. It is distributed from Coquimbo to the Valparaíso Region. We analyzed karyotypes of 10 populations along its natural distribution. All the populations presented an asymmetric karyotype, with 2n = 16 chromosomes but with three different karyotypic formulae. Alstroemeria magnifica var. magnifica and A. magnifica var. sierrae presented the same karyotypic fomula, and A. magnifica var....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alstroemeria; Karyotype; Species complex; Asymmetry; Chile.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300434

Genetic variants in the G gamma-globin promoter modulate fetal hemoglobin expression in the Colombian population
Provedor de dados: Genetics and Molecular Biology
Autores: Fong,Cristian; Mendoza,Yesica; Barreto,Guillermo.
Abstract Fetal hemoglobin (HbF) is a determining factor for the development of sickle cell anemia. High HbF levels lower the intensity of symptoms of this disease. HbF levels can vary in patients with sickle cell anemia and individuals without the disease. The purpose of this study was to identify the genetic variants in the G gamma-globin gene promoter that can modulate HbF expression in patients with sickle cell anemia and healthy individuals from Colombia. In total, 413 bp of the G gamma-globin gene promoter were sequenced in 60 patients with sickle cell anemia and 113 healthy individuals. The allelic and genotype frequencies of the identified variants were compared between individuals with low and high HbF for both patients and healthy individuals. In...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sickle cell anemia; Fetal hemoglobin; Gamma globin; Regulation of gene expression; Colombia.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400103

Analysis of genetic population structure in Acacia caven (Leguminosae, Mimosoideae), comparing one exploratory and two Bayesian-model-based methods
Provedor de dados: Genetics and Molecular Biology
Autores: Pometti,Carolina L.; Bessega,Cecilia F.; Saidman,Beatriz O.; Vilardi,Juan C.
Bayesian clustering as implemented in STRUCTURE or GENELAND software is widely used to form genetic groups of populations or individuals. On the other hand, in order to satisfy the need for less computer-intensive approaches, multivariate analyses are specifically devoted to extracting information from large datasets. In this paper, we report the use of a dataset of AFLP markers belonging to 15 sampling sites of Acacia caven for studying the genetic structure and comparing the consistency of three methods: STRUCTURE, GENELAND and DAPC. Of these methods, DAPC was the fastest one and showed accuracy in inferring the K number of populations (K = 12 using the find.clusters option and K = 15 with a priori information of populations). GENELAND in turn, provides...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acacia caven; AFLP; GENELAND; DAPC.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100012

Genetic parameters of wool colour and skin traits in Corriedale sheep
Provedor de dados: Genetics and Molecular Biology
Autores: Benavides,M.V.; Maher,P.
Clean wool colour (CWC) is an important wool price determinant and has been related to suint characteristics, i.e. sudoriparous and sebaceous gland secretions, such as suint percentage and suint K content. In this work heritability, phenotypic and genetic correlations among wool colour and skin traits were examined. The genetic estimates were assessed by Restricted Maximum Likelihood (REML) procedures using average information algorithm (AIREML) in a Corriedale flock. The traits analysed were wool colour traits (CWC), yellow predictive colour (YPC), and Visual Score; suint traits such as suint percentage and potassium and sodium concentrations in suint, and physiological traits such as potassium and sodium concentrations in the skin, including plasma and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wool yellowing; Indirect selection criteria; Sheep.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300009

Ras oncogene and Hypoxia-inducible factor-1 alpha (hif-1α) expression in the Amazon fish Colossoma macropomum (Cuvier, 1818) exposed to benzo[a]pyrene.
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Grazyelle Sebrenski da; Fé,Luciana Mara Lopes; Silva,Maria de Nazaré Paula da; Val,Vera Maria Fonseca de Almeida e.
Abstract Benzo[a]pyrene (B[a]P) is a petroleum derivative capable of inducing cancer in human and animals. In this work, under laboratory conditions, we analyzed the responses of Colossoma macropomum to B[a]P acute exposure through intraperitoneal injection of four different B[a]P concentrations (4, 8, 16 and 32 μmol/kg) or corn oil (control group). We analyzed expression of the ras oncogene and the Hypoxia-inducible factor-1 alpha (hif-1α) gene using quantitative real-time PCR. Additionally, liver histopathological changes and genotoxic effects were evaluated through the comet assay. Ras oncogene was overexpressed in fish exposed to 4, 8 of 16 μmol/kg B[a]P, showing 4.96, 7.10 and 6.78-fold increases, respectively. Overexpression also occurred in hif-1α...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ras oncogene; Hif-1α; Tambaqui; B[a]P; Genotoxic damage.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300491

Choice of common bean parents based on combining ability estimates
Provedor de dados: Genetics and Molecular Biology
Autores: Machado,Cristina de Fátima; Santos,João Bosco dos; Nunes,Glauber Henrique de Sousa; Ramalho,Magno Antonio Patto.
The obtention of genetically improved cultivars is the main objective of breeding programs, whose efficiency is increased by a careful choice of parents. Based on both general (GCA) and specific (SCA) combining abilities for grain yield, the purpose of this research was to choose the most promising populations of common beans, for line selection. GCA and SCA were measured by means of a complete diallel without reciprocals, using twelve cultivars/lines. Parents and corresponding F2 segregating populations were evaluated for grain yield by a 9 x 9 triple square lattice design. It was found that the segregating populations differed in grain yield, with predominant SCA effects, but with significant GCA effects as well. Among the populations derived from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Specific and general combining ability; Diallel.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200011

Molecular characterization and genetic structure of the Nero Siciliano pig breed
Provedor de dados: Genetics and Molecular Biology
Autores: Guastella,Anna Maria; Criscione,Andrea; Marletta,Donata; Zuccaro,Antonio; Chies,Luigi; Bordonaro,Salvatore.
Nero Siciliano is an autochthonous pig breed that is reared mainly in semi-extensive systems in northeastern Sicily. Despite its economic importance and well-appreciated meat products, this breed is currently endangered. Consequently, an analysis of intra-breed variability is a fundamental step in preserving this genetic resource and its breeding system. In this work, we used 25 microsatellite markers to examine the genetic composition of 147 unrelated Nero Siciliano pigs. The total number of alleles detected (249, 9.96 per locus) and the expected heterozygosity (0.708) indicated that this breed had a high level of genetic variability. Bayesian cluster analysis showed that the most likely number of groups into which the sample could be partitioned was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cluster analysis; Coancestry; Intra-breed genetic variation; Microsatellites; Nero Siciliano pig breed.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400010

Association between interleukin 6 -174 G/C promoter gene polymorphism and runners' responses to the dietary ingestion of antioxidant supplementation based on pequi (Caryocar brasiliense Camb.) oil: a before-after study
Provedor de dados: Genetics and Molecular Biology
Autores: Miranda-Vilela,Ana Luisa; Ribeiro,Ieler Ferreira; Grisolia,Cesar Koppe.
Abstract Exercise is a double-edged sword: when practiced in moderation, it increases the expression of antioxidant enzymes, but when practiced strenuously it causes oxidative stress and cell damage. In this context, polymorphisms in the interleukin (IL)-6 gene should be investigated better because they can influence performance, at least in exercise that generates oxidative stress and leads to muscular injuries with consequent inflammation. In this work, we investigated the influence of IL-6 –174 G/C polymorphism on tissue damage and inflammation markers, lipid peroxidation, hemogram and lipid profile of runners before and after ingestion of 400 mg of pequi oil in capsules supplied daily for 14 consecutive days. The IL-6 genotypes were associated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant supplementation; Exercise-induced oxidative damage; Inflammatory markers; Nutrigenetics; Nutrigenomics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400554

Directed mutagenesis affects recombination in Azospirillum brasilense nif genes
Provedor de dados: Genetics and Molecular Biology
Autores: Nunes,C.P.; Passaglia,L.M.P.; Schrank,A.; Schrank,I.S.
In order to improve the gene transfer/mutagenesis system for Azospirillum brasilense, gene-cartridge mutagenesis was used to replace the nifD gene with the Tn5 kanamycin resistance gene. The construct was transferred to A. brasilense by electrotransformation. Of the 12 colonies isolated using the suicide plasmid pSUP202 as vector, only four did not show vector integration into the chromosome. Nevertheless, all 12 colonies were deficient in acetylene reduction, indicating an Nif- phenotype. Four Nif- mutants were analyzed by Southern blot, using six different probes spanning the nif and Km r genes and the plasmid vector. Apparently, several recombination events occurred in the mutant genomes, probably caused mainly by gene disruption owing to the...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400032

Genomic imprinting: genetic mechanisms and phenotypic consequences in Prader-Willi and Angelman syndromes
Provedor de dados: Genetics and Molecular Biology
Autores: Fridman,Cintia; Koiffmann,Célia P.
Chromosomal 15q11-q13 region is of great interest in Human Genetics because many structural rearrangements have been described for it (deletions, duplications and translocations) leading to phenotypes resulting in conditions such as the Prader-Willi (PWS) and Angelman (AS) syndromes which were the first human diseases found to be related to the differential expression of parental alleles (genomic imprinting). Contrary to Mendelian laws where the parental inheritance of genetic information does not influence gene expression, genomic imprinting is characterized by DNA modifications that produce different phenotypes depending on the parental origin of the mutation. Clinical manifestation of PWS appears when the loss of paternally expressed genes occurs and AS...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400004

The bone marrow micronucleus test and metronidazole genotoxicity in different strains of mice (Mus musculus)
Provedor de dados: Genetics and Molecular Biology
Autores: Abrevaya,Ximena C.; Carballo,Marta A.; Mudry,Marta D.
The mouse (Mus musculus) bone marrow micronucleus test was carried out using 24 outbred National Institutes of Health (NIH) mice, 24 inbred Swiss Webster (CFW) mice and 20 inbred Bagg albino/color locus Jackson (BALB/cJ) mice. The mice in the experimental group (n = 32) were injected intraperitoneally with 133 mg kg-1 of metronidazole parenteral solution and the control group consisted of mice (n = 36) which had not been injected with metronidazole. There was no significant difference (p &gt; 0.05) between the sexes regarding the micronucleus frequency in either the experimental or the control group. When the Mn frequencies of the three strains were compared, the results for the CFW and BALB/cJ strains did not differ statistically (p &gt; 0.05) for either...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BALB/cJ; Bone marrow micronucleus test; CFW; Genotoxicity; Metronidazole; NIH.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600018

Comparison of HMOX1 expression and enzyme activity in blue-shelled chickens and brown-shelled chickens
Provedor de dados: Genetics and Molecular Biology
Autores: Wang,ZhePeng; Liu,RuiFang; Wang,AnRu.
Blue egg coloring is attributed to biliverdin derived from the oxidative degradation of heme through catalysis by heme oxygenase (HO). The pigment is secreted into the eggshell by the shell gland. There is uncertainty as to whether the pigment is synthesized in the shell gland or in other tissues. To investigate the site of pigment biosynthesis, the expression of heme oxygenase (decycling) 1 (HMOX1), a gene encoding HO, and HO activity in liver and spleen were compared between blue-shelled chickens (n=12) and brown-shelled chickens (n=12). There were no significant differences in HMOX1 expression and HO activity in these tissues between the two groups. Since the liver and spleen, two important sites outside the shell gland where heme is degraded into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biliverdin; Blue egg; Chicken; HMOX1.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200020

Beyond retrovirus infection: HIV meets gene therapy
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Flávia Helena da; Dalberto,Tiago Pires; Nardi,Nance Beyer.
The human immunodeficiency virus (HIV) is classified as a retrovirus because of its RNA genome and the fact that it requires reverse transcriptase to convert it into DNA. This virus belongs to the lentivirinae subfamily and is able to infect quiescent cells but is better known for its association with acquired immunodeficiency syndrome (AIDS) and can be described as one of the most effective vectors for gene transfer. Biosafety concerns are present whenever viral vectors are employed but are particularly pertinent to the development of HIV-based vectors. Insertional mutagenesis and the production of new replication-competent viruses (RCV) have been pointed to as major problems, but experimental data have shown that safe protocols can be developed for their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; MLV; Retrovirus; Lentivirus; Gene therapy.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200027

MHC class I loci of the Bar-Headed goose (Anser indicus)
Provedor de dados: Genetics and Molecular Biology
Autores: Liang,Qinglong; Wei,Lei; Wang,Xinwei; He,Hongxuan.
MHC class I proteins mediate functions in anti-pathogen defense. MHC diversity has already been investigated by many studies in model avian species, but here we chose the bar-headed goose, a worldwide migrant bird, as a non-model avian species. Sequences from exons encoding the peptide-binding region (PBR) of MHC class I molecules were isolated from liver genomic DNA, to investigate variation in these genes. These are the first MHC class I partial sequences of the bar-headed goose to be reported. A preliminary analysis suggests the presence of at least four MHC class I genes, which share great similarity with those of the goose and duck. A phylogenetic analysis of bar-headed goose, goose and duck MHC class I sequences using the NJ method supports the idea...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MHC I; Anser indicus; Polymorphism; Phylogenetic analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300031

Connexins in the early development of the African clawed frog Xenopus laevis (Amphibia): The role of the connexin43 carboxyl terminal tail in the establishment of the dorso-ventral axis
Provedor de dados: Genetics and Molecular Biology
Autores: Cofre,Jaime; Abdelhay,Eliana.
Connexins are a family of related proteins identified in vertebrate forming gap junctions, which mediate cell-to-cell communication in early embryos, with an important role in establishing embryonic asymmetry and ‘communication compartments’. By in situ hybridization, immunocytochemistry, reverse transcriptase PCR (RT-PCR) and western blotting we show that a Cx43-like molecule is present in oocytes and embryos of the African clawed frog Xenopus laevis, with specific localization in the animal-vegetal axis. This specific distribution is suggestive for an important role for this protein in the establishment of the dorso-ventral axis. Antisense RNA and antibodies directed against rat carboxyl terminal tail of the Cx43 (CT-Cx43) and injected in 1-cell stage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Connexins; Carboxyl terminal tail; Dorso-ventral axis; Early development in amphibians; Xenopus laevis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300030

Colombian Creole horse breeds: same origin but different diversity
Provedor de dados: Genetics and Molecular Biology
Autores: Jimenez,Ligia Mercedes; Mendez,Susy; Dunner,Susana; Cañón,Javier; Cortés,Óscar.
In order to understand the genetic ancestry and mitochondrial DNA (mtDNA) diversity of current Colombian horse breeds we sequenced a 364-bp fragment of the mitocondrial DNA D-loop in 116 animals belonging to five Spanish horse breeds and the Colombian Paso Fino and Colombian Creole cattle horse breeds. Among Colombian horse breeds, haplogroup D had the highest frequency (53%), followed by haplogroups A (19%), C (8%) and F (6%). The higher frequency of haplogroup D in Colombian horse breeds supports the theory of an ancestral Iberian origin for these breeds. These results also indicate that different selective pressures among the Colombian breeds could explain the relatively higher genetic diversity found in the Colombian Creole cattle horse when compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colombian; Creole cattle horse; Horse breeds; Mitochondrial DNA; Paso Fino horse breed.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500013

Cytogenetics and sperm ultrastructure of Atelopus spumarius (Anura, Bufonidae) from the Brazilian Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Siqueira,Sérgio; Aguiar Junior,Odair; Lima,Albertina Pimentel; Recco-Pimentel,Shirlei Maria.
The current taxonomy of most Atelopus species is based on morphological and color data only. Recent studies suggest that A. spumarius may represent a species complex assigned under the same name. Karyotypic data and description of sperm ultrastructure for 13 specimens of A. spumarius are presented here for the first time. A chromosomal analysis revealed 2n = 22 chromosomes, with centromeric heterochromatin in all pairs and a nucleolar organizer region (NOR) on the telomere of pair 7. The sperm was of the bufonoid type, presenting a filiform nucleus covered by an acrosomal complex and a mitochondrial collar in the neck region. The tail was composed of an axoneme, an undulating membrane and an axial rod. A karyotype analysis of A. spumarius showed the same...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ag-NOR; Atelopus spumarius; C-band; Chromosome; Sperm ultrastructure.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400011

Draft genome of Thermomonospora sp. CIT 1 (Thermomonosporaceae) and in silico evidence of its functional role in filter cake biomass deconstruction
Provedor de dados: Genetics and Molecular Biology
Autores: Omori,Wellington P.; Pinheiro,Daniel G.; Kishi,Luciano T.; Fernandes,Camila C.; Fernandes,Gabriela C.; Gomes-Pepe,Elisângela S.; Pavani,Claudio D.; Lemos,Eliana G. de M.; Souza,Jackson A. M. de.
Abstract The filter cake from sugar cane processing is rich in organic matter and nutrients, which favors the proliferation of microorganisms with potential to deconstruct plant biomass. From the metagenomic data of this material, we assembled a draft genome that was phylogenetically related to Thermomonospora curvata DSM 43183, which shows the functional and ecological importance of this bacterium in the filter cake. Thermomonospora is a gram-positive bacterium that produces cellulases in compost, and it can survive temperatures of 60 ºC. We identified a complete set of biomass depolymerizing enzymes in the draft genome of Thermomonospora sp. CIT 1, such as α-amylase, catalase-peroxidases, β-mannanase, and arabinanase, demonstrating the potential of this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actinobacteria; Pectin; Hemicellulose; Crystalline cellulose; Orthologous genes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100145

Linkage disequilibrium, genetic association mapping and gene localization in crop plants
Provedor de dados: Genetics and Molecular Biology
Autores: Sorkheh,Karim; Malysheva-Otto,Lyudmyla V.; Wirthensohn,Michelle G.; Tarkesh-Esfahani,Saeed; Martínez-Gómez,Pedro.
DNA-based molecular markers have been extensively utilized for a variety of studies in both plant and animal systems. One of the major uses of these markers is the construction of genome-wide molecular maps and the genetic analysis of simple and complex traits. However, these studies are generally based on linkage analysis in mapping populations, thus placing serious limitations in using molecular markers for genetic analysis in a variety of plant populations. Therefore, alternative approach has been suggested, linkage disequilibrium-based association analysis which detects and locates quantitative trait loci (QTL) by the strength of the correlation between a trait and a marker. Although association analysis has already been used for studies on genetics of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Linkage disequilibrium; LD; Association mapping; Population structure; QTLs; Localization of genes; Marker-trait associations.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500001

Comparative cytogenetics among populations of Astyanax altiparanae (Characiformes, Characidae, Incertae sedis)
Provedor de dados: Genetics and Molecular Biology
Autores: Ferreira Neto,Maressa; Vicari,Marcelo Ricardo; Camargo,Edemar Furquim de; Artoni,Roberto Ferreira; Moreira-Filho,Orlando.
Cytogenetic data are presented for Astyanax altiparanae populations from three Brazilian hydrographic systems. The chromosomal data obtained in A. altiparanae support the hypothesis of diploid number conservation. However, small differences in the karyotype formula and number of nucleolar organizer regions were observed in these populations. The apparent karyotypical similarity among the studied populations strongly suggests a close relationship among them with some chromosomal divergences due to gene flow restriction.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotaxonomy; Karyotype diversification; Fish cytogenetics.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400021

A functional polymorphism in the paired basic amino acid-cleaving enzyme 4 gene confers osteoarthritis risk in a population of Eastern China
Provedor de dados: Genetics and Molecular Biology
Autores: He,Jin; Yang,Haoyu; Xu,Zhonghua; Li,Jin; Chen,Gang; Jiang,Lifeng; Wu,Lidong; Zhou,Xindie.
Abstract Paired basic amino acid-cleaving enzyme 4 (PACE4), a proprotein convertase, is involved in the activation of aggrecanases (ADAMTS-4 and ADAMTS-5) in osteoarthritic and cytokine-stimulated cartilage. Activated aggrecanases cause aggrecan degradation and thus, contribute to osteoarthritis (OA). In this study, we investigated the association between PACE4 gene polymorphisms and OA risk. One single-nucleotide polymorphism (rs4965833) in the PACE4 gene was genotyped in 432 OA patients and 523 healthy controls using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Quantitative reverse transcription PCR (qRT-PCR) was used to determine the relative expression of PACE4 in blood samples from 90 OA patients (30 for each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ACE4; Osteoarthritis; Bioinformatics analysis; Single-nucleotide polymorphism.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100104

Association of MYF5 gene allelic variants with production traits in pigs
Provedor de dados: Genetics and Molecular Biology
Autores: Carmo,Fausto Moreira da Silva; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Pires,Aldrin Vieira; Guimarães,Marta Fonseca Martins; Silva,Marcos Vinícius Gualberto Barbosa da; Schierholt,Alex Sandro; Silva,Kleibe de Moraes e; Gomide,Lúcio Alberto de Miranda.
We studied the phenotypic effects of polymorphisms at the MYF5 gene in a divergent F2 swine population and found that one polymorphism was due to an insertion and another to a deletion. The genotypes of 359 F2 animals were obtained and the Normal/Normal (NN) and Normal/Insertion (NI) genotypes analyzed to determine associations with phenotypic data for performance, carcass and meat quality traits. Significant differences were observed (p &lt; 0.05) between NN and NI animals for drip (NN = 3.14 ± 1.56; NI = 3.69 ± 2.78%), cooking (NN = 32.26 ± 2.41; NI = 33.21 ± 2.31%) and total loss (NN = 34.16 ± 2.63 and NI = 34.97 ± 2.08%). The Deletion marker was not statistically tested. The results indicate that the allelic variant Insertion is associated with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myogenesis; DNA sequencing; Pig production; PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300004

Can plant DNA barcoding be implemented in species-rich tropical regions? A perspective from São Paulo State, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Renato A. Ferreira de; Oliveira,Alexandre Adalardo de; Colletta,Gabriel Dalla; Flores,Thiago Bevilacqua; Coelho,Rubens L. Gayoso; Dias,Pedro; Frey,Gabriel Ponzoni; Iribar,Amaia; Rodrigues,Ricardo Ribeiro; Souza,Vinícius Castro; Chave,Jérôme.
Abstract DNA barcoding helps to identify species, especially when identification is based on parts of organisms or life stages such as seeds, pollen, wood, roots or juveniles. However, the implementation of this approach strongly depends on the existence of complete reference libraries of DNA sequences. If such a library is incomplete, DNA-based identification will be inefficient. Here, we assess if DNA barcoding can already be implemented in species-rich tropical regions. We focus on the tree flora of São Paulo state, Brazil, which contains more than 2000 tree species. Using new DNA sequence data and carefully assembled GenBank accessions, we assembled 12,113 sequences from ten different regions. The ITS, rbcL, psbA-trnH, matK and trnL regions were better...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity assessment; Plant barcoding; Species conservation; Tree species.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400661

CUG-BP and 3'UTR sequences influence PARN-mediated deadenylation in mammalian cell extracts
Provedor de dados: Genetics and Molecular Biology
Autores: Moraes,Karen C.M.; Wilusz,Carol J.; Wilusz,Jeffrey.
Several mRNAs have been shown to exhibit distinct patterns of poly(A) shortening prior to their decay in vivo. In this study, we show that individual transcripts also demonstrate distinct patterns of deadenylation in in vitro systems derived from HeLa and Jurkat T cell cytoplasmic extracts. The major patterns observed were slow/synchronous and fast/asynchronous poly(A) tail shortening. For all RNA substrates tested, PARN was shown to be the enzyme responsible for the deadenylation patterns that were observed. Sequences in the 3' untranslated regions influenced the deadenylation pattern. Using a fragment of the 3'UTR of the c-fos mRNA as a model, the interaction of CUG-BP, the human homolog of EDEN-BP - a protein previously implicated in regulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRNA decay; Deadenylation; MRNA stability; 3'UTR; CUG-BP.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400024

Multiple pericentric inversions and chromosomal divergence in the reef fishes Stegastes (Perciformes, Pomacentridae)
Provedor de dados: Genetics and Molecular Biology
Autores: Molina,Wagner Franco; Galetti Jr.,Pedro Manoel.
Damselfishes (Pomacentridae, Perciformes) occur in all major oceans of the world and, with approximately 320 species, represent one of the most diverse families of marine Teleostei. The taxonomy of these reef fishes is problematic because of the large number of complex species and the range of color patterns they display, which vary among individuals and populations of the same species. In this study, we examined the cytogenetic composition of four species of Stegastes (S. pictus, S. fuscus, S. variabilis and S. leucostictus) found along the coast of Brazil. Stegastes pictus had a chromosomal number of 2n = 48 (14m+28sm+2st+4a, fundamental number (FN) = 92), S. fuscus had 2n = 48 (20m+22sm+6a, FN = 90), S. variabilis had 2n = 48 (18m+22sm+8a, FN = 88), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pericentric inversions; Pomacentridae; Stegastes; 5S genes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400013

Beta-globin gene cluster haplotypes in the Mapuche Indians of Argentina
Provedor de dados: Genetics and Molecular Biology
Autores: Kaufman,Letícia; Carnese,Francisco R.; Goicoechea,Alicia; Dejean,Cristina; Salzano,Francisco M.; Hutz,Mara H.
Haplotypes derived from five polymorphic restriction sites in the beta-globin gene cluster were investigated in 86 chromosomes from the Argentinian Mapuche. These results were integrated with those previously obtained for ten Brazilian Indian tribes. Eight haplotypes were identified, the most frequent being 2 (57%) and 6 (27%). The presence of haplotype 3 in 2% of the Mapuche chromosomes is probably an evidence of admixture with individuals of African ancestry. Due to the high number of haplotypes observed, heterozygosity as measured by the Gini-Simpson index was higher in the Mapuche than in Brazilian Indians. The haplotypic distribution in the Mapuche was also significantly different from those of all Brazilian tribes investigated. This heterogeneity...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400003

Elastin (ELN) gene point mutation in patients with inguinal hernia
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Consuelo Junqueira; Yoo,Jin Hwan; Rodrigues Junior,Aldo Junqueira.
Groin hernias emerge at the myopectineal orifice of Fruchaud which is closed off by the fascia transversalis. Our previous studies showed structural and quantitative changes of the fascia transversalis elastic fibers of inguinal hernia patients and elderly people. The present study used single-strand conformation polymorphism (SSCP) elastin (analysis to investigate the 34 exons of the ELN gene of 49 inguinal hernia patients (7 females, 42 males aged 58.7 ± 19.82 years) and 75 non-herniated controls (35 females, 40 males aged 46.2 ± 14.32 years). We found that 47 patients and 24 controls had an abnormal exon 20 pattern caused by a g28197A &gt; G missense mutation leading to an S422G amino acid substitution in the elastin hydrophobic domain. The g28197A &gt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fascia transversalis; Extracellular matrix; Elastic fibers; Elastin gene; Inguinal hernia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100009

Nuclear DNA content of thirty species of Neotropical fishes
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Margarida Lima; Oliveira,Claudio; Foresti,Fausto.
The present paper reports nuclear DNA content in 30 Neotropical freshwater fish species and summarizes the data on other Neotropical species presented in the literature. Among Neotropical fishes, the nuclear DNA content ranges from 1.04 ± 0.09 pg/nucleus in Corydoras cf. simulatus (2n = 62) to 248.0 pg/nucleus in Lepidosiren paradoxa (2n = 38). A general analysis of the data obtained in the present study for each species showed that DNA measurements were practically constant at the individual level, while significant differences were observed among individuals of the same population. This observation was valid for all species analyzed and was more evident in those species that presented other karyotypic particularities such as sex chromosomes or...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100009

Analysis of the SHP2 enhancer for the use of tissue specific activation tagging in Arabidopsis thaliana
Provedor de dados: Genetics and Molecular Biology
Autores: Chalfun-Junior,Antonio; Mes,Jurriaan J.; Busscher,Marco; Angenent,Gerco C.
Activation tagging is a powerful tool to identify new mutants and to obtain information about possible biological functions of the overexpressed genes. The quadruple cauliflower mosaic virus (CaMV) 35S enhancer fragment is a strong enhancer, which is most commonly used for this purpose. However, the constitutive nature of this enhancer may generate lethal mutations or aberrations in different plant organs by the same overexpressed gene. A tissue-specific activation tagging approach may overcome these drawbacks and may also lead more efficiently to the desired phenotype. For this reason the SHATTERPROOF2 (SHP2) promoter fragment was analysed for enhancer activity. The SHP2 gene is involved in dehiscence zone development and expressed during silique...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arabidopsis; Development; Pod shattering; Transcription factors; Mutants.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200032

Does the expression of the ACVR2A gene affect the development of colorectal cancer?
Provedor de dados: Genetics and Molecular Biology
Autores: Wodziński,Damian; Wosiak,Agnieszka; Pietrzak,Jacek; Świechowski,Rafał; Jeleń,Agnieszka; Balcerczak,Ewa.
Abstract Colorectal cancer has become a serious problem, especially in highly developed countries. As reported by the World Health Organization, the number of colon cancer cases in the world in 2012 amounted to 1.36 million. It is the second most common cancer in females (614,000 cases, 9.2% of the total) and the third in males (746,000 cases, 10.0% of the total) worldwide. It is believed that TGFβ pathway elements are involved in the pathogenesis of colorectal cancer. This study assessed one of these elements, the ACVR2A gene. Qualitative and quantitative analyses of the ACVR2A gene in 84 patients with colorectal cancer was performed. There was no statistically significant association between ACVR2A gene expression and age, gender, histological type,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Activin A protein; ACVR2A gene expression; Tumor suppressor; Colorectal cancer; TGFß signaling pathway.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100032

Mitodepressive and clastogenic effects of aqueous extracts of the lichens Myelochroa lindmanii and Canoparmelia texana (Lecanorales, Parmeliaceae) on meristematic cells in plant bioassays
Provedor de dados: Genetics and Molecular Biology
Autores: Campos,José Marcello Salabert de; Davide,Lisete Chamma; Soares,Geraldo Luiz Gonçalves; Viccini,Lyderson Facio.
The cytotoxicity effect of aqueous extracts of the lichens species Myelochroa lindmanii and Canoparmelia texana (Lecanorales, Parmeliaceae) were evaluated using meristematic cells of lettuce (Lactuca sativa) and maize (Zea mays). The seasonal effect was also evaluated. Extracts of M. lindmanii and C. texana inhibited root growth and/or percentage germination, possibly due to alterations in the cell cycle. The M. lindmanii extract showed anti-mitotic effects and blocked the cell cycle in metaphase so that c-mitosis and cells with chromosome duplication were produced. The C. texana extract appeared to hinder cell division, increasing the number of interphase cells. In addition, both extracts caused an increase in percentage of cell death. Clastogenic effects...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Canoparmelia texana; Cytotoxicity; Mitotic index; Myelochroa lindmanii; Plant bioassays.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100024

A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Elisete Pains; Torres,Adalgisa Ribeiro; Batista,Jesiane Stefânia da Silva; Huergo,Luciano; Hungria,Mariangela.
Sample preparation is a critical step in two-dimensional gel electrophoresis (2-DE) of plant tissues. Here we describe a phenol/SDS procedure that, although greatly simplified, produced well-resolved and reproducible 2-DE profiles of protein extracts from soybean [Glycine max (L.) Merril] roots. Extractions were made in three replicates using both the original and simplified procedure. To evaluate the quality of the extracted proteins, ten spots were randomly selected and identified by mass spectrometry (MS). The 2-DE gels were equally well resolved, with no streaks or smears, and no significant differences were observed in protein yield, reproducibility, resolution or number of spots. Mass spectra of the ten selected spots were compared with database...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2-DE; Protein extraction; Proteomics; Soybean roots; Symbiosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200016

Variation in extragenic repetitive DNA sequences in Pseudomonas syringae and potential use of modified REP primers in the identification of closely related isolates
Provedor de dados: Genetics and Molecular Biology
Autores: Çepni,Elif; Gürel,Filiz.
In this study, Pseudomonas syringe pathovars isolated from olive, tomato and bean were identified by species-specific PCR and their genetic diversity was assessed by repetitive extragenic palindromic (REP)-PCR. Reverse universal primers for REP-PCR were designed by using the bases of A, T, G or C at the positions of 1, 4 and 11 to identify additional polymorphism in the banding patterns. Binding of the primers to different annealing sites in the genome revealed additional fingerprint patterns in eight isolates of P. savastanoi pv. savastanoi and two isolates of P. syringae pv. tomato. The use of four different bases in the primer sequences did not affect the PCR reproducibility and was very efficient in revealing intra-pathovar diversity, particularly in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial identification; Biodiversity; PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400016

Complex segregation analysis of nonsyndromic cleft lip/palate in a Chilean population
Provedor de dados: Genetics and Molecular Biology
Autores: Blanco,Rafael; Arcos-Burgos,Mauricio; Paredes,Mónica; Palomino,Hernán; Jara,Lilian; Carreño,Hernán; Obreque,Victor; Muñoz,M.A.
A população urbana Chilena contemporânea deriva da mistura de ameríndios nativos com espanhóis, apresentando uma incidência média de fissura labial não sindrômica associada ou não a fissura palatina (NSCLP) de 1,8 por 1000 nascimentos vivos.  A análise de segregação complexa usando o programa de computador POINTER foi feita em 249 pedigrees estendidos, distribuídos em 202 famílias simplex e 47 famílias multiplex obtidas de probands de NSCLP afetados (157 homens e 92 mulheres). Esses pedigrees deram origem a 326 indivíduos afetados e mais de 1454 parentes.  Oito modelos hipotéticos foram examinados e comparados pelo teste &lt;FONT FACE="Symbol"&gt;c&lt;/font&gt; 2 log2 razão de máxima verossimilhança.  Os modelos que postulam que NSCLP não era transmitida...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100023

Bovine gene polymorphisms related to fat deposition and meat tenderness
Provedor de dados: Genetics and Molecular Biology
Autores: Fortes,Marina R.S.; Curi,Rogério A.; Chardulo,Luis Artur L.; Silveira,Antonio C.; Assumpção,Mayra E.O.D.; Visintin,José Antonio; Oliveira,Henrique N. de.
Leptin, thyroglobulin and diacylglycerol O-acyltransferase play important roles in fat metabolism. Fat deposition has an influence on meat quality and consumers' choice. The aim of this study was to determine allele and genotype frequencies of polymorphisms of the bovine genes, which encode leptin (LEP), thyroglobulin (TG) and diacylglycerol O-acyltransferase (DGAT1). A further objective was to establish the effects of these polymorphisms on meat characteristics. We genotyped 147 animals belonging to the Nelore (Bos indicus), Canchim (5/8 Bos taurus + 3/8 Bos indicus), Rubia Gallega X Nelore (1/2 Bos taurus + 1/2 Bos indicus), Brangus Three-way cross (9/16 Bos taurus + 7/16 Bos indicus) and Braunvieh Three-way cross (3/4 Bos taurus + 1/4 Bos indicus)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine gene polymorphisms; Candidate gene; Fat deposition; Beef cattle.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100011

Minimal prevalence of Huntington’s disease in the South of Brazil and instability of the expanded CAG tract during intergenerational transmissions
Provedor de dados: Genetics and Molecular Biology
Autores: Castilhos,Raphael Machado de; Santos,José Augusto dos; Augustin,Marina Coutinho; Pedroso,José Luiz; Barsottini,Orlando; Saba,Roberta; Ferraz,Henrique Ballalai; Godeiro Junior,Clécio; Vargas,Fernando Regla; Salarini,Diego Zanotti; Furtado,Gabriel Vasata; Polese-Bonatto,Marcia; Rodrigues,Luiza Paulsen; Sena,Lucas Schenatto; Saraiva-Pereira,Maria Luiza; Jardim,Laura Bannach.
Abstract Huntington’s disease (HD) is due to dominant expansions of the CAG repeat of the HTT gene. Meiotic instability of the (CAG)n might impact the disorder frequency. We report on HD minimal prevalence in Rio Grande do Sul (RS) state, Brazil, and on intergenerational instability of the (CAG)n in HD families. Symptomatic and at-risk subjects from 179 HD families were ascertained between 2013 and 2016. Clinical, molecular and family history data were obtained. Expanded (CAG)n length differences between parent and child (delta-expanded-(CAG)n) were calculated. Effect of parental age on the (CAG)n instability upon transmission was inferred by correlating delta-expanded-(CAG)n between siblings to their age differences. HD minimal prevalence in RS state was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CAG expansion; Huntington’s disease; Intergenerational instability; Minimal prevalence.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300329

Combination of two rare mutations causes β-thalassaemia in a Bangladeshi patient
Provedor de dados: Genetics and Molecular Biology
Autores: Moosa,Mahdi Muhammad; Ayub,Mustak Ibn; Bashar,AMA Emran; Sarwardi,Golam; Khan,Waqar; Khan,Haseena; Yeasmin,Sabina.
Screening of mutations that cause β-thalassaemia in the Bangladeshi population led to the identification of a patient with a combination of two rare mutations, Hb Monroe and HBB: -92C&gt;G.The β-thalassaemia major male individual was transfusion-dependent and had an atypical β-globin gene cluster haplotype. Of the two mutations, Hb Monroe has been characterized in detail. Clinical effects of the other mutation, HBB: -92C&gt;G,are unknown so far. Bioinformatics analyses were carried out to predict the possible effect of this mutation. These analyses revealed the presence of a putative binding site for Egr1, a transcription factor, within the HBB:-92 region. Our literature survey suggests a close relationship between different phenotypic manifestations of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hb Monroe HBB: -92C&gt; G transcription factor Egr1.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300006

The effect of magnesium on mitotic spindle formation in Schizosaccharomyces pombe
Provedor de dados: Genetics and Molecular Biology
Autores: Uz,Gulsen; Sarikaya,Aysegul Topal.
Abstract Magnesium (Mg2+), an essential ion for cells and biological systems, is involved in a variety of cellular processes, including the formation and breakdown of microtubules. The results of a previous investigation suggested that as cells grow the intracellular Mg2+ concentration falls, thereby stimulating formation of the mitotic spindle. In the present work, we used a Mg2+-deficient Schizosaccharomyces pombe strain GA2, in which two essential membrane Mg2+ transporter genes (homologs of ALR1 and ALR2 in Saccharomyces cerevisae) were deleted, and its parental strain Sp292, to examine the extent to which low Mg2+ concentrations can affect mitotic spindle formation. The two S. pombe strains were transformed with a plasmid carrying a GFP-α2-tubulin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnesium; Microtubule; Mitosis; Mitotic spindle; Schizosaccharomyces pombe.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300459

Diverse coactivator recruitment through differential PPARγ nuclear receptor agonism
Provedor de dados: Genetics and Molecular Biology
Autores: Lizcano,Fernando; Vargas,Diana.
The PPARγ nuclear receptor regulates the expression of genes involved in lipid and carbohydrate metabolism, and it has protective effects in some patients with type 2 diabetes. Nevertheless, the therapeutic value of the PPARγ nuclear receptor protein is limited due to the secondary effects of some PPARγ ligands. Because the downstream effects of PPARγ are determined by the binding of specific cofactors that are mediated by ligand-induced conformational changes, we evaluated the differential effects of various ligands on the binding of certain cofactors associated with PPARγ. The ligands used were rosiglitazone for treating type 2 diabetes and telmisartan for treating arterial hypertension. Functional, phenotypic, and molecular studies were conducted on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA binding proteins co-activator; Gene expression; Transcription; Telmisartan.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100020

Genotoxic effects of Tabebuia impetiginosa (Mart. Ex DC.) Standl. (Lamiales, Bignoniaceae) extract in Wistar rats
Provedor de dados: Genetics and Molecular Biology
Autores: Lemos,Odilon A.; Sanches,Júlio C.M.; Silva,Ícaro E.F.; Silva,Márcio L.A.; Vinhólis,Adriana H.C.; Felix,Mireille A.P.; Santos,Raquel A.; Cecchi,Andréa O.
Tabebuia sp. is native to tropical rain forests throughout Central and South America. Although the biological and pharmacological effects of bark extracts have been intensely studied, little is known on the extract obtained from the flower. Herein, the genotoxic potential of a flower extract from T. impetiginosa ("ipê roxo") on the blood and liver cells of Wistar rats was evaluated. Experimental procedures involved only male animals. Graduated concentrations of the extract, viz., 100, 300 and 500 mg kg-1 of body weight, were gavage-administered and 24 h latter cells were collected and processed for analysis. With the exception of the 100 mg kg-1 dose, a significant increase in DNA damage was noted, when compared with a negative control group. Although the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tabebuia impetiginosa; Comet assay; Wistar rats; Natural compounds.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300017

Population genetic structure of Brazilian shrimp species (Farfantepenaeus sp., F. brasiliensis, F. paulensis and Litopenaeus schmitti: Decapoda: Penaeidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Gusmão,Jaqueline; Lazoski,Cristiano; Solé-Cava,Antonio M.
Penaeid shrimps are important resources for worldwide fisheries and aquaculture. In the Southwest Atlantic, Farfantepenaeus brasiliensis, F. paulensis, F. subtilis, Farfantepenaeus sp. and Litopenaeus schmitti are among the most important commercially exploited species. Despite their high commercial value, there is little information available on the different aspects of their biology or genetics and almost no data on their stock structure. We used allozymes to estimate variability levels and population genetic structure of F. brasiliensis, F. paulensis, L. schmitti and the recently detected species Farfantepenaeus sp. along as much as 4,000 km of Brazilian coastline. No population heterogeneity was detected in F. brasiliensis or L. schmitti along the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Farfantepenaeus; Litopenaeus; Allozymes.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100029

Evaluation of extracts from Coccoloba mollis using the Salmonella/microsome system and in vivo tests
Provedor de dados: Genetics and Molecular Biology
Autores: Tsuboy,Marcela Stefanini; Marcarini,Juliana Cristina; Ferreira,Dalva Trevisan; Ferraz,Elisa Raquel Anastácio; Chequer,Farah Maria Drumond; Oliveira,Danielle Palma de; Ribeiro,Lúcia Regina; Mantovani,Mário Sérgio.
The common everyday use of medicinal plants is an ancient, and still very widespread practice, whereby the need for studies on their possible toxicity and mutagenic properties. The species Coccoloba mollis has been much used in phytotherapy, mainly in cases involving loss of memory and stress. In order to investigate its genotoxic and mutagenic potential, ethanolic extracts from the leaves and roots underwent Salmonella/microsome assaying (TA98 and TA100 strains, with and without exogenous metabolism - S9), besides comet and micronucleus tests in vivo.There was no significant increase in the number of revertants/plate of Salmonella strains in any of the analyzed root-extract concentrations, although the extract itself was extremely toxic to the Salmonella...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Coccoloba mollis; Ames test; In vivo comet assay; In vivo micronucleus assay; Polygonaceae.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300027

Sugarcane genes related to mitochondrial function
Provedor de dados: Genetics and Molecular Biology
Autores: Fonseca,Ghislaine V.; Tambor,José Humberto M.; Nobrega,Marina P.; Santos,Rafael; Nobrega,Francisco G.
Mitochondria function as metabolic powerhouses by generating energy through oxidative phosphorylation and have become the focus of renewed interest due to progress in understanding the subtleties of their biogenesis and the discovery of the important roles which these organelles play in senescence, cell death and the assembly of iron-sulfur (Fe/S) centers. Using proteins from the yeast Saccharomyces cerevisiae, Homo sapiens and Arabidopsis thaliana we searched the sugarcane expressed sequence tag (SUCEST) database for the presence of expressed sequence tags (ESTs) with similarity to nuclear genes related to mitochondrial functions. Starting with 869 protein sequences, we searched for sugarcane EST counterparts to these proteins using the basic local...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100024

Differential gene expression between wild-type and Gulo-deficient mice supplied with vitamin C
Provedor de dados: Genetics and Molecular Biology
Autores: Jiao,Yan; Zhang,Jifei; Yan,Jian; Stuart,John; Gibson,Griffin; Lu,Lu; Willaims,Robert; Wang,Yong Jun; Gu,Weikuan.
The aim of this study was to test the hypothesis that hepatic vitamin C (VC) levels in VC deficient mice rescued with high doses of VC supplements still do not reach the optimal levels present in wild-type mice. For this, we used a mouse scurvy model (sfx) in which the L-gulonolactone oxidase gene (Gulo) is deleted. Six age- (6 weeks old) and gender- (female) matched wild-type (WT) and sfx mice (rescued by administering 500 mg of VC/L) were used as the control (WT) and treatment (MT) groups (n = 3 for each group), respectively. Total hepatic RNA was used in triplicate microarray assays for each group. EDGE software was used to identify differentially expressed genes and transcriptomic analysis was used to assess the potential genetic regulation of Gulo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; L-gulonolactone oxidase; Liver; Oxidative stress; Vitamin C.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300004

Methodological differences can affect sequencing depth with a possible impact on the accuracy of genetic diagnosis
Provedor de dados: Genetics and Molecular Biology
Autores: Borges,Murilo G.; Rocha,Cristiane S.; Carvalho,Benilton S.; Lopes-Cendes,Iscia.
Abstract For a better interpretation of variants, evidence-based databases, such as ClinVar, compile data on the presumed relationships between variants and phenotypes. In this study, we aimed to analyze the pattern of sequencing depth in variants from whole-exome sequencing data in the 1000 Genomes project phase 3, focusing on the variants present in the ClinVar database that were predicted to affect protein-coding regions. We demonstrate that the distribution of the sequencing depth varies across different sequencing centers (pair-wise comparison, p &lt; 0.001). Most importantly, we found that the distribution pattern of sequencing depth is specific to each facility, making it possible to correctly assign 96.9% of the samples to their sequencing center....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Whole exome sequencing; Depth; ClinVar; Computational biology; Clinical genomics.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400804

Genetic variability of Hypostomus (Teleostei, Loricariidae) from the Ribeirão Maringá, a stream of the upper Rio Paraná basin, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Paiva,Suzana de; Renesto,Erasmo; Zawadzki,Cláudio Henrique.
Hypostomus strigaticeps and two morphotypes of Hypostomus were collected from Ribeirão Maringá, a small tributary of the Rio Pirapó, an effluent of the upper Rio Paraná. The three populations were analyzed by allozyme electrophoresis that allowed the scoring of 25 loci from 14 enzyme systems. Heterozygosity values (He) were 0.028 in H. strigaticeps, 0.027 in Hypostomus sp. 1 and zero in Hypostomus sp. 2. Several diagnostic loci and fixed differences were observed for each population at loci Acp-A, Gcdh-A and Mdhp-A. Thus, all populations were genetically distinct, although there were many common alleles. The unbiased genetic identities of Nei (I) were estimated as 0.780 for Hypostomus sp. 1 and H. strigaticeps, 0.357 for H. strigaticeps and Hypostomus sp....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allozymes; Heterozygosity; Hypostomus; Loricariidae; Systematic.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300005

Developing a common bean core collection suitable for association mapping studies
Provedor de dados: Genetics and Molecular Biology
Autores: Perseguini,Juliana Morini Küpper Cardoso; Silva,Gliciane Micaele Borges; Rosa,João Ricardo Bachega Feijó; Gazaffi,Rodrigo; Marçal,Jéssica Fernanda; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando; Zucchi,Maria Imaculada; Garcia,Antonio Augusto Franco; Benchimol-Reis,Luciana Lasry.
Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Genetic diversity; Genetic structure; Microsatellites; Phaseolus vulgaris L.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100067

Erratum
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/report
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400023

Transposable elements P and gypsy in natural populations of Drosophila willistoni
Provedor de dados: Genetics and Molecular Biology
Autores: Sassi,Adriana Koslovski; Herédia,Fabiana; Loreto,Élgion Lucio da Silva; Valente,Vera Lucia da Silva; Rohde,Claudia.
The presence and integrity of the P transposon and the gypsy retrotransposon in the genome of 18 samples of natural Drosophila willistoni populations collected from a large area of South America were Southern blot screened using Drosophila melanogaster probes. The aim of this screening was provide further knowledge-base on the geographical distribution of D. willistoni and to carry out an inter-population analysis of the P and gypsy elements present in the genomes of the populations analyzed. The fragment patterns obtained indicate that both the P and gypsy elements are ancient in the D. willistoni genome, but whereas the gypsy retroelement appears to be invariable and stable the P element varies between populations and appears to still have some capacity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila willistoni; Transposable element; P; Gypsy.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500013

Association between MUC1 gene polymorphism and expected progeny differences in Nelore cattle (Bos primigenius indicus)
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Fabio Ricardo Pablos de; Vozzi,Pedro Alejandro; Vila,Reginaldo Aparecido; Boligon,Arione Augusti; Galerani,Marli Aparecida Vani; Lobo,Raysildo Barbosa; Martelli,Lucia Regina.
MUC1 is a heavily glycosylated mammalian transmembrane protein expressed by mucosal secretory tissues for both protection against microbial infection and lubrication. An important characteristic of MUC1 is its variable number of tandem repeats (VNTR) containing several sites for O-glycosylation. VNTR length has been associated with many human diseases and with certain economically important traits in domestic ruminants. The aim of the present study was to correlate the length of MUC1 gene VNTR with expected progeny differences (EPDs) obtained for growth, fertility and carcass traits. Five alleles were identified, with alleles containing short VNTRs being more frequent than those with long, thereby demonstrating that Brazilian Nelore cattle are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Molecular marker; Productive traits; Quantitative trait locus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100013

Gender-dependent association of HSD11B1 single nucleotide polymorphisms with glucose and HDL-C levels
Provedor de dados: Genetics and Molecular Biology
Autores: Turek,Luciane Viater; Leite,Neiva; Souza,Ricardo Lehtonen Rodrigues; Lima,Jovana Karoline; Milano,Gerusa Eisfeld; Timossi,Luciana da Silva; Osiecki,Ana Claudia Vecchi; Osiecki,Raul; Alle,Lupe Furtado.
In this study, we investigated the influence of two SNPs (rs846910 and rs12086634) of the HSD11B1 gene that encodes 11β-hydroxysteroid dehydrogenase type 1(11β-HSD1), the enzyme that catalyzes the conversion of cortisol to cortisone, on variables associated with obesity and metabolic syndrome in 215 individuals of both sexes from southern Brazil. The HSD11B1 gene variants were genotyped using the TaqMan SNP genotyping assay. Glucose, triglycerides, total cholesterol, HDL-cholesterol and LDL-cholesterol were measured by standard automated methods. Significant results were found in women, with carriers of the G allele of SNP rs12086634 having higher glucose levels than non-carriers. Carriers of the A allele of SNP rs846910 had higher levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HSD11B1 gene; Men; Metabolism; Metabolic syndrome; Women.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400003

In situ hybridization of bat chromosomes with human (TTAGGG)n probe, after previous digestion with Alu I
Provedor de dados: Genetics and Molecular Biology
Autores: Faria,Karina de Cassia; Morielle-Versute,Eliana.
The purpose of this work was to verify the ability of the enzyme Alu I to cleave and/or remove satellite DNA sequences from heterochromatic regions in chromosomes of bats, by identifying the occurrence of modifications in the pattern of fluorescence in situ hybridization with telomeric DNA. The localization and fluorescence intensity of the telomeric DNA sites of the Alu-digested and undigested chromosomes of species Eumops glaucinus, Carollia perspicillata, and Platyrrhinus lineatus were analyzed. Telomeric sequences were detected at the termini of chromosomes of all three species, although, in C. perspicillata, the signals were very faint or absent in most chromosomes. This finding was interpreted as being due to a reduced number of copies of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AluI; FISH; Chiroptera; Telomeric sites; DAPI.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400003

A rapid technique for analysis of formalin-fixed, paraffin-embedded tissues by fluorescent in situ hybridization with alpha-satellite probes
Provedor de dados: Genetics and Molecular Biology
Autores: Barril,Nilce; Carvalho-Salles,Andréa Borduchi; Tajara,Eloiza Helena.
We describe a rapid procedure for preparing archival tissues for interphase FISH analysis. The present protocol differs from others previously described because it allows the obtention of nuclei in satisfactory number and quality without using special equipments, adhesive-treated slides or solutions for chromatin decondensation. The method is of low cost and useful for retrospective analyses of formalin-fixed, paraffin-embedded samples.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400006

Profile of micronucleus frequencies and nuclear abnormalities in different species of electric fishes (Gymnotiformes) from the Eastern Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Melo,Karina Motta; Alves,Ingrid Reale; Pieczarka,Julio Cesar; David,José Augusto de Oliveira; Nagamachi,Cleusa Yoshiko; Grisolia,Cesar Koppe.
The frequency of spontaneous micronucleus (MN) formation in fish species needs to be determined to evaluate their usefulness for genotoxic biomonitoring. The definition of a good bioindicator takes into account the current knowledge of its metabolic traits as well as other factors including its feeding behavior and relationship to the environment. In this study, we compared the basal frequencies of micronucleated erythrocytes and nuclear abnormalities (NA) among different species of the fish Order Gymnotiformes (Rhamphichthys marmoratus, Steatogenys elegans, Sternopygus macrurus, Parapteronotus hasemani, Gymnotus mamiraua, Gymnotus arapaima, Brachyhypopomus beebei, Brachyhypopomus n. sp. BENN) sampled in several localities of the Eastern Amazon. A baseline...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomonitoring; Genotoxicity; Gymnotiformes; Amazon region; Fish erythrocytes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300018

Micronucleus frequencies in Astyanax bimaculatus (Characidae) treated with cyclophosphamide or vinblastine sulfate
Provedor de dados: Genetics and Molecular Biology
Autores: Matsumoto,F.E.; Cólus,I.M.S.
Two known mutagenic drugs, cyclophosphamide and vinblastine sulfate, were tested using the micronucleus test in the native fish species, Astyanax bimaculatus, in order to determine which of these drugs and the doses which would be the most adequate for use as positive controls in this species. This Brazilian fish species was chosen because few toxicity studies have used native fish species and this particular species is widely consumed in various regions of Brazil. Three thousand erythrocytes per specimen were scored. Doses of 16 and 8 mg/kg body weight of cyclophosphamide and vinblastine sulfate, respectively, were the most effective in causing micronuclei. Cyclophosphamide was the most mutagenic of the two drugs and is recommended for use as a positive...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200041

Quantitative trait loci (QTL) mapping for growth traits on bovine chromosome 14
Provedor de dados: Genetics and Molecular Biology
Autores: Miyata,Marcelo; Gasparin,Gustavo; Coutinho,Luiz Lehmann; Martinez,Mario Luiz; Machado,Marco Antonio; Silva,Marcos Vinicius G. Barbosa da; Campos,Ana Lucia; Sonstegard,Tad S.; Rosário,Millor Fernandes do; Regitano,Luciana Correia de Almeida.
Quantitative trait loci (QTL) mapping in livestock allows the identification of genes that determine the genetic variation affecting traits of economic interest. We analyzed the birth weight and weight at 60 days QTL segregating on bovine chromosome BTA14 in a F2 resource population using genotypes produced from seven microsatellite markers. Phenotypes were derived from 346 F2 progeny produced from crossing Bos indicus Gyr x Holstein Bos taurus F1 parents. Interval analysis to detect QTL for birth weight revealed the presence of a QTL (p &lt; 0.05) at 1 centimorgan (cM) from the centromere with an additive effect of 1.210 ± 0.438 kg. Interval analysis for weight at 60 days revealed the presence of a QTL (p &lt; 0.05) at 0 cM from the centromere with an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TA14; Cattle; F2; Growth traits; Molecular markers; QTL.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300011

Transformation of the entomopathogenic fungi, paecilomyces fumosoroseus and paecilomyces lilacinus (deuteromycotina: hyphomycetes) to benomyl resistence
Provedor de dados: Genetics and Molecular Biology
Autores: Inglis,Peter W.; Tigano,Myrian S.; Valadares-Inglis,M. Cléria.
The entomopathogenic fungi Paecilomyces fumosoroseus and P. lilacinus have been transformed to resistance to the fungicide benomyl by a polyethylene glycol (PEG)-mediated procedure using a mutant &lt;FONT FACE="Symbol"&gt;b&lt;/font&gt;-tubulin gene from Neurospora crassa carried on plasmid pBT6. Benomyl-resistant transformants of P. lilacinus were obtained that could tolerate greater than 30 µg/ml benomyl and P. fumosoroseus transformants were obtained that could tolerate 20 µg/ml benomyl. Following 5 serial passages of transformants on benomyl-containing media and 5 serial passages on non-selective media, 100% of P. lilacinus transformants were found to be mitotically stable by a conidial germination test. In contrast, only 4 out of 9 transformants of P....
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100023

Comparative cytogenetic and morphological analysis of Astyanax scabripinnis paranae (Pisces, Characidae, Tetragonopterinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Maistro,Edson Luis; Oliveira,Claudio; Foresti,Fausto.
Cytogenetic and morphological studies were carried out on nine local populations of Astyanax scabripinnis paranae. All populations exhibited 2n = 50 chromosomes as well as conspicuous differences involving karyotype morphology, number and position of nucleolar organizer regions (NORs) and amount and/or locations of constitutive heterochromatin blocks. A quantitative study of the cytogenetic data showed that eight populations possessed different karyotypes. Morphological analyses based on nine measurements and two meristic parameters were effective in establishing clear identification of five populations. Comparative analysis of cytogenetic and morphological traits suggests that chromosomal changes have occurred at a more rapid rate than morphological...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200005

Matrix metalloproteinase gene polymorphisms in patients with coronary artery disease
Provedor de dados: Genetics and Molecular Biology
Autores: Dalepiane,Vanessa L.N.; Silvello,Daiane N.; Paludo,Crislaine A.; Roisenberg,Israel; Simon,Daniel.
Matrix metalloproteinases (MMPs) play an important role in the pathogenesis of atherosclerosis, the pathology underlying the majority of coronary artery disease (CAD). In this study we tested the hypothesis that polymorphic variation in the MMP genes influences the risk of developing atherosclerosis. We analyzed functional polymorphisms in the promoter of the MMP-1, MMP-3, MMP-9 and MMP-12 genes in 183 Brazilian Caucasian individuals submitted to coronary angiography, of which 67 (37%) had normal coronary arteries (control group) and 116 (63%) had CAD (CAD patient group). The -1607 1G/2G MMP-1, -1171 5A/6A MMP-3, -1562 C/T MMP-9, -82 A/G MMP-12 polymorphisms were analyzed by PCR followed by restriction digestion. No significant differences were observed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Coronary artery disease; Gene polymorphisms; Matrix metalloproteinases.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400001

Population structure of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from Central Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Daniela de Melo e; Cruz,Aparecido Divino da; Bastos,Rogério Pereira; Reis,Raquel Loren; Telles,Mariana Pires de Campos; Diniz-Filho,José Alexandre Felizola.
This study reports on 156 specimens of the amphibian Eupemphix nattereri, a widely distributed leiuperid, obtained from 11 municipalities of central Brazil. The extent of genetic variation was quantified by determining the mean number of alleles per locus and the proportion of polymorphic loci. An analysis of molecular variance (AMOVA) was performed on the random amplified polymorphic DNA (RAPD) haplotypes. The genetic distances obtained by calculating pairwise phist among local samples were used to determine population relationships using the unweighted pair-group method (UPGMA) and non-metric multidimensional scaling (NMDS). The cophenetic correlation was calculated to confirm agreement between the genetic matrix and the unweighted pair group method with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eupemphix nattereri; Gene flow; Population structure; RAPD markers.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600022

Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Roxane Wirschum; Freitas,Thales R.O. de; Sbalqueiro,Ives José.
In this study, the microsatellite technique was used to evaluate the genetic variability in populations of collared and white-lipped peccaries kept in captivity. Six primers developed for domestic pigs were used and amplified in both species. They revealed the presence of five polymorphic loci and one monomorphic locus. The polymorphic loci included 4 of the 16 alleles in collared peccaries, and 3 of the 10 alleles in the white-lipped peccaries. Polymorphic information content (PIC) in both species and all the loci was highly informative. The probability of paternity exclusion (PEC), if one of the parents is known, was almost as high in white-lipped peccaries (95.53%) as in the collared (99,48%). The Fst values for collared (0.042) and white-lipped...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite markers; Tayassu pecari; Pecari tajacu; Genetic variability; Animals of captivity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100012

HOX  gene analysis in the osteogenic differentiation of human mesenchymal stem cells
Provedor de dados: Genetics and Molecular Biology
Autores: Chae,Song Wha; Jee,Bo Keun; Lee,Joo Yong; Han,Chang Whan; Jeon,Yang-Whan; Lim,Young; Lee,Kweon-Haeng; Rha,Hyoung Kyun; Chae,Gue-Tae.
Human bone marrow-derived mesenchymal stem cells (hMSCs) have the capacity to differentiate into osteoblasts during osteogenesis. Several studies attempted to identify osteogenesis-related genes in hMSCs. Although  HOX  genes are known to play a pivotal role in skeletogenesis, their function in the osteogenesis of hMSCs has not yet been investigated in detail. Our aim was to characterize the expression of 37  HOX  genes by multiplex RT-PCR to identify the ones most probably involved in osteogenic differentiation. The results showed that the expression patterns of four  HOX  genes were altered during this process. In particular, the expression levels of  HOXC13  and  HOXD13  were dramatically changed. Real-time PCR and Western blot analysis were performed...
Palavras-chave: Human mesenchymal stem cells; HOX  genes; Osteogenic differentiation; Stem cell differentiation; Gene profiling.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500002

Metabolic adjustments in Satanoperca aff. jurupari (Perciformes: Cichlidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Chippari-Gomes,Adriana R.; Leitão,Marco Aurélio B.; Paula-Silva,Maria de Nazaré; Mesquita-Saad,Lenise S. B. de; Almeida-Val,Vera Maria F.
In this paper, we describe the enzyme levels and isozyme distribution in skeletal and heart muscle of Satanoperca aff. jurupari, (Cichlidae, subgroup Geophaginae). LDH and CS were measured in skeletal and heart muscle. Starch gel electrophoresis was used to determine the isozyme/allozyme patterns in different tissues; LDH, MDHs, PGM, PGI, ADH, G-6-PDH and SOD were screened for the numbers of loci, presence of alleles, and tissue specificity. The LDH/CS ratio in heart and skeletal muscle were 173.36 and 6.1, respectively, indicating anaerobic metabolism in the former and aerobic metabolism in the latter muscle. No inhibition by pyruvate (based on the ratios of LDH activity with 1 mM and 10 mM pyruvate) was detected in heart and skeletal muscle, indicating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cichlid; Plasticity; Metabolism; Enzymes; Amazon.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100005

Cytotaxonomy and karyoevolution of the genus Crenicichla (Perciformes, Cichlidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Benzaquem,Denise Corrêa; Feldberg,Eliana; Porto,Jorge Ivan Rebelo; Gross,Maria Claudia; Zuanon,Jansen Alfredo Sampaio.
Karyotypes of six cichlid species of the genus Crenicichla were investigated. The species C. cincta, C. inpa, C. reticulata, C. lugubris, and C. cf. johanna were collected from Amazon basin, and C. britskii was collected from the Paraná-Paraguai basin. All of the analysed species showed 2n = 48 chromosomes; however, C. cincta, C. lugubris, C. cf. johanna, and C. britskii had a karyotype formula of 8M-SM+40ST-A, FN = 56, while C. inpa and C. reticulata exhibited the formula 6M-SM+42ST-A, FN = 54. Analysis of active Ag-NORs revealed two NOR-bearing chromosomes in all species; however, theses cistrons were located on different chromosome pairs and/or in different chromosome locations in each species. This condition bears evolutionary significance, since it is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Pike cichlid; Chromosome marker; Amazon.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200016

Gene variation and genetic differentiation among populations of the solitary mud dauber wasp Trypoxylon (Trypargilum) albitarse Fabricius 1804 (Hymenoptera, Crabronidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Bergamaschi,Antonio C.B.; Lama,Marco A. Del.
Abstract Trypoxylon is a genus of solitary crabronid wasps whose population genetics is poorly known. The purpose of the present study was to investigate the genetic variation and differentiation among five populations of Trypoxylon albitarse, a species widely distributed throughout the Neotropics, with records from Panama to northern Argentina. Eight species-specific microsatellite loci were used for genotyping 96 adult wasps (one female per nest) sampled at five sites in Brazil. The analysis of allelic richness and private alleles indicated high genetic diversity in the populations sampled. Pairwise comparisons using the Fst and Dest indices revealed significant differentiation for all, but one pair of populations. Fst, Dest, AMOVA and assignment test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population genetics; Wasps; Crabronidae; SSR markers.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400519

Phylogenetic relationships between Arabidopsis and sugarcane bZIP transcriptional regulatory factors
Provedor de dados: Genetics and Molecular Biology
Autores: Vincentz,Michel; Schlögl,Paulo S.; Corrêa,Luis Gustavo G.; Kühne,Fabiana; Leite,Adilson.
We built a complete and non-redundant database of bZIP transcriptional regulatory factors from the Arabidopsis reference genome. These Arabidopsis bZIP factors were ordered into thirteen families of evolutionary related proteins and this classification was used to identify and organize sugarcane cDNAs encoding bZIP proteins. We also show how this classification should help in defining putative clusters of orthologous groups of higher plant bZIP regulators and briefly discuss the expected benefits of this procedure to efficiently characterize sugarcane bZIP transcriptional regulators.
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100009

Cytotoxic and genotoxic potential of surface water from the Pitimbu river, northeastern/RN Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Egito,Lucila Carmem Monte; Medeiros,Maria das Graças; Medeiros,Silvia Regina Batistuzzo de; Agnez-Lima,Lucymara Fassarella.
In this study, the onion (Allium cepa) root test was used to evaluate the genotoxicity of the Pitimbu River (Natal city, Brazil) surface water. The water was collected at five sampling sites along the river and one sample was obtained after the treatment (flocculation, chlorination and pH correction) of the river water for human consumption. All raw river water samples increased the frequency of chromosomal abnormalities and/or micronuclei and two of the water samples produced alterations in the mitotic index of the root cells. Two of the water samples also altered root growth and two produced morphological modifications in the A. cepa roots. Water collected from a site near an industrial area was the most consistently toxic and genotoxic of the samples....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Micronucleus; Cytotoxicity; Genotoxicity; Surface water.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300023

Structure and genetic relationships between Brazilian naturalized and exotic purebred goat domestic goat (Capra hircus) breeds based on microsatellites
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Joelliton Domingos de; Igarashi,Maria Luiza Silveira de Paiva; Machado,Théa Mírian Medeiros; Miretti,Marcos Mateo; Ferro,Jesus Aparecido; Contel,Eucleia Primo Betioli.
The genetic relationships and structure of fourteen goat (Capra hircus) populations were estimated based on genotyping data from 14 goat populations (n = 410 goats) at 13 microsatellite loci. We used analysis of molecular variance (AMOVA), principal component analysis (PCA) and F statistics (F IS, F IT and F ST) to evaluate the genetic diversity (Ho, He and ad) of the goats. Genetic distances between the 14 goat populations were calculated from allelic frequency data for the 13 microsatellite markers. Moderate differentiation was observed for the populations of the undefined breeds (including the Anglo-Nubian-M breed), the naturalized Brazilian breeds (Moxotó, Canindé), the exotic purebred breeds (Alpine, Saanen, Toggenbourg and Anglo-Nubian) and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goat; Microsatellite; Genetic distance; PCA; AMOVA; Genetic relationships; Genetic diversity; F statistics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300010

Milk and fat production of crossbred Holstein-Gir cows (Bos taurus taurus-Bos taurus indicus) in the agreste region of the Brazilian state of Pernambuco
Provedor de dados: Genetics and Molecular Biology
Autores: Barbosa,Severino B.P.; Ramalho,Ricardo P.; Monardes,Humberto G.; Dias,Flávio M.; Santos,Djalma C. dos; Batista,Ângela M.V.
The productive performance of four Holstein-Gir genetic groups (1/2H, 1/4H, 5/8H, 5/8Hinter se) and the effects of non-genetic factors on production traits was evaluated using a data set of 7,951 test-day samples collected between January 1980 and December 1999 from the Arcoverde Experimental Station in the Brazilian state of Pernambuco (Empresa Pernambucana de Pesquisa Agropecuária). The statistical model included the fixed effects of calving year (1980 to 1999) and month (January to December), genetic group of the cow, age at months of calving (33 to 190) and days in milk at each test-day. Random effects were sire, cow and residual with test-days treated as repeated records within each cow within each lactation. For the four genetic groups the milk yield...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic groups; Epistasis; Genetics groups; Recombination; Repeated measures; Test-day.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300012

Isozyme and RAPD studies in Prosopis glandulosa and P. Velutina (Leguminosae, Mimosoideae)
Provedor de dados: Genetics and Molecular Biology
Autores: Bessega,Cecilia; Saidman,Beatriz O.; Vilardi,Juan C.
Allozyme and random amplified polymorphic DNA (RAPD) techniques have been compared for their usefulness for genetic and taxonomic studies in Prosopis glandulosa and P. velutina populations. Isozymes and RAPDs yielded similarly high estimates of genetic variability. Genetic structure and differentiation were analyzed through non-hierarchical Wright's F DT. For all populations considered, both markers produced low gene flow (Nm &lt; 1) estimates. When only P. glandulosa populations were analyzed, isozyme data yielded higher gene flow estimates (Nm &gt; 1), in agreement with that expected for conspecific populations. However, in RAPD data the expected reduction in F DT and the increase in Nm were not observed. Correlation between F DT and geographical...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300024

Use of ridge regression for the prediction of early growth performance in crossbred calves
Provedor de dados: Genetics and Molecular Biology
Autores: Pimentel,Eduardo da Cruz Gouveia; Queiroz,Sandra Aidar de; Carvalheiro,Roberto; Fries,Luiz Alberto.
The problem of multicollinearity in regression analysis was studied. Ridge regression (RR) techniques were used to estimate parameters affecting the performance of crossbred calves raised in tropical and subtropical regions by a model including additive, dominance, joint additive or "profit heterosis" and epistatic effects and their interactions with latitude in an attempt to model genotype by environment interactions. A software was developed in Fortran 77 to perform five variant types of RR: the originally proposed method; the method implemented by SAS; and three methods of weighting the RR parameter lambda. Three mathematical criteria were tested with the aim of choosing a value for the lambda coefficient: the sum and the harmonic mean of the absolute...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crossbreeding; Epistasis; Genotype by environment interaction; Heterosis; Multicollinearity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400006

Analysis of mutations in the cystic fibrosis transmembrane regulator (CFTR) gene in patients with obstructive azoospermia
Provedor de dados: Genetics and Molecular Biology
Autores: Bernardino,Andrea L.F.; Lima,Cintia E.; Zatz,Mayana.
Congenital bilateral absence of the vas deferens (CBAVD) accounts for 1%-2% of sterility in men. A high incidence of mutations, as well as the involvement of the 5T variant of the T tract length in intron 8 of the cystic fibrosis conductance regulator (CFTR) gene, have been previously described in males with CBAVD. Herein we report the screening for mutations and for the 5T variant of the CFTR gene in 17 patients with CBAVD and three others with non-CABVD obstructive azoospermia. In the CBAVD group, three patients (15%) were compound heterozygotes for mutations, and five patients (25%) had a mutation in one allele and the 5T variant in the other; the 5T variant was also present in two other patients, one of them being homozygous. The most frequent mutation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CFTR mutations; Obstructive azoospermia; Male infertility.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100001

Genetic control of Eucalyptus urophylla and E. grandis resistance to canker caused by Chrysoporthe cubensis
Provedor de dados: Genetics and Molecular Biology
Autores: Guimarães,Lúcio Mauro da Silva; Resende,Marcos Deon Vilela de; Lau,Douglas; Rosse,Leonardo Novaes; Alves,Alexandre Alonso; Alfenas,Acelino Couto.
Chrysophorte cubensis induced canker occurs in nearly all tropical and subtropical regions where eucalypts are planted, causing losses in both wood quality and volume productivity, especially so in the warmer and more humid regions of Brazil. The wide inter and intra-specific genetic variability of resistance to canker among Eucalyptus species facilitates the selection of resistant plants. In this study, we evaluated resistance to this pathogen in five Eucalyptus grandis (G) and 15 E. urophylla (U) trees, as well as in 495 individuals from 27 progenies derived from crosses between the trees. In the field, six-months-old test seedlings were inoculated with C. cubensis. Lesion length in the xylem and bark was measured eight months later. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic resistance; Genetic breeding; Eucalypt and interspecific hybrids.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300024

Molecular cloning and mRNA expression of the peptidoglycan recognition protein gene HcPGRP1 and its isoform HcPGRP1a from the freshwater mussel Hyriopsis cumingi
Provedor de dados: Genetics and Molecular Biology
Autores: Tao,Ye; Yang,Zi-yan; Zhang,Xin; Wu,Hong-juan.
Peptidoglycan recognition proteins (PGRPs) are innate immune molecules that have been structurally conserved throughout evolution in invertebrates and vertebrates. In this study, peptidoglycan recognition protein HcPGRP1 and its isoform HcPGRP1a were identified in the freshwater mussel Hyriopsis cumingii. The full-length cDNAs of HcPGRP1 (973 bp) and HcPGRP1a (537 bp) encoded polypeptides with 218 and 151 amino acids, respectively. Sequence analysis showed that HcPGRP1 had one C-terminal PGRP domain that was conserved throughout evolution. Phylogenetic analysis showed that HcPGRP1 clustered closely with EsPGRP4 of Euprymna scolopes. Real-time PCR showed that the mRNA transcripts of HcPGRP1 and HcPGRP1a were constitutively expressed in various tissues, with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Expression pattern; Gene cloning; Hyriopsis cumingi; Innate immunity; Peptidoglycan recognition protein.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400006

Association between reproductive traits and four microsatellites in Brangus-Ibagé cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,João F.C. de; Neves,Jairo P.; Almeida,Eric A.; Steigleder,Clara Sabina; Moraes,José Carlos F.; Gonçalves,Paulo Bayard D.; Weimer,Tania A.
The aim of the present study was to verify associations between reproductive efficiency and four microsatellite markers located in synteny with genes involved in the regulation of reproductive mechanisms. A sample of 107 females from a Brangus Ibagé population (5/8 Aberdeen Angus x 3/8 Nelore) was characterized for ETH225 (D9S1) and MM12E6 (D9S20) microsatellites, mapped on chromosome 9, and HEL5 (D21S15) and AFZ1 (D21S37) on chromosome 21. Associations between the genetic markers and reproductive efficiency were determined by one-way analysis of variance using calving interval (CI), live weight at calving (LWC), live weight at first calving (LW1C) and live weight at second calving (LW2C) as dependent variables. The genotypes were classified according to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; IGF-I receptor; Estrogen receptor; Reproductive efficiency.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100010

A genomic approach to characterization of the Citrus terpene synthase gene family
Provedor de dados: Genetics and Molecular Biology
Autores: Dornelas,Marcelo Carnier; Mazzafera,Paulo.
Terpenes are a very large and structurally diverse group of secondary metabolites which are abundant in many essential oils, resins and floral scents. Additionally, some terpenes have roles as phytoalexins in plant-pathogen relationships, allelopathic inhibitors in plant-plant interactions, or as airborne molecules of plant-herbivore multitrophic signaling. Thus the elucidation of the biochemistry and molecular genetics of terpenoid biosynthesis has paramount importance in any crop species. With this aim, we searched the CitEST database for clusters of expressed sequence tags (ESTs) coding for terpene synthases. Herein is a report on the identification and in silico characterization of 49 putative members of the terpene synthase family in diverse Citrus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Secondary metabolism; Flavor; Aroma; Plant protection; Terpene synthesis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500011

NFAT5 is differentially expressed in Sprague-Dawley rat tissues in response to high salt and high fructose diets
Provedor de dados: Genetics and Molecular Biology
Autores: Herman,Braden A.; Ferguson,Kaylee M.; Fernandez,Jared V.B.; Kauffman,Samantha; Spicher,Jason T.; King,Rachel J.; Halterman,Julia A.
Abstract Current diets contain an increasing amount of salt and high fructose corn syrup, but it remains unclear as to how dietary salt and fructose affect organ function at the molecular level. This study aimed to test the hypothesis that consumption of high salt and fructose diets would increase tissue-specific expression of two critical osmotically-regulated genes, nuclear factor of activated T-cells 5 (NFAT5) and aldose reductase (AR). Fifty Sprague-Dawley rats were placed on a control, 4% NaCl, 8% NaCl, or 64% fructose diet for eight weeks. Fourteen different tissue samples were harvested and snap-frozen, followed by RNA purification, cDNA synthesis, and NFAT5 and AR gene expression quantification by real-time PCR.Our findings demonstrate that NFAT5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NFAT5; Aldose reductase; Salt; Fructose; Real-time PCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300452

Self-reported cancer family history is a useful tool for identification of individuals at risk of hereditary cancer predisposition syndrome at primary care centers in middle-income settings: a longitudinal study
Provedor de dados: Genetics and Molecular Biology
Autores: Flória-Santos,Milena; Lopes-Júnior,Luís Carlos; Alvarenga,Larissa de Melo; Ribeiro,Mayara Segundo; Ferraz,Victor Evangelista de Faria; Nascimento,Lucila Castanheira; Pereira-da-Silva,Gabriela.
Abstract Analysis of cancer family history (CFH) offers a low-cost genetic tool to identify familial cancer predisposition. In middle-income settings, the scarcity of individual records and database-linked records hinders the assessment of self-reported CFH consistency as an indicator of familial cancer predisposition. We used self-reported CFH to identify those families at risk for hereditary cancer syndromes in community-based primary care centers of a low-income Brazilian area. We also evaluated the consistency of the information collected by reassessing CFH five years later. We interviewed 390 families and constructed their pedigrees for genetic cancer risk assessment. We found 125 families affected by cancer, 35.2% with moderate to high risk of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic predisposition to disease; Risk factors; Neoplasms; Family; Pedigree.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200178

Analysis of paternal lineages in Brazilian and African populations
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Mónica; Brito,Pedro; Lopes,Virgínia; Andrade,Lisa; Anjos,Mª João; Real,Francisco Corte; Gusmão,Leonor.
The present-day Brazilian population is a consequence of the admixture of various peoples of very different origins, namely, Amerindians, Europeans and Africans. The proportion of each genetic contribution is known to be very heterogeneous throughout the country. The aim of the present study was to compare the male lineages present in two distinct Brazilian populations, as well as to evaluate the African contribution to their male genetic substrate. Thus, two Brazilian population samples from Manaus (State of Amazon) and Ribeirão Preto (State of São Paulo) and three African samples from Guinea Bissau, Angola and Mozambique were typed for a set of nine Y chromosome specific STRs. The data were compared with those from African, Amerindian and European...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome Y; STRs; Lineages; Brazil; Africa.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300004

Estimates of genetic parameters related to chitinase production by the entomopathogenic fungus Metarhizium anisopliae
Provedor de dados: Genetics and Molecular Biology
Autores: Braga,Gilberto U.L.; Vencovsky,Roland; Messias,Claudio L.
Chitinolytic activity and dry mass production were determined in culture filtrates from 17 Metarhizium anisopliae strains grown in liquid medium containing chitin as the only carbon source. The objectives were to estimate parameters such as genetic variance among strains, heritability and expected gain from selection, as well as correlations between tested traits. Wide genotypic variability was observed among strains in chitinolytic activity, permitting the exploitation of this property in selection. The high heritability suggests that progress can be made through phenotypic selection. The genotypic correlation coefficient between dry mass production and chitinolytic activity detected in the filtrates was negative (&lt;img...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200001

Biomolecular computers with multiple restriction enzymes
Provedor de dados: Genetics and Molecular Biology
Autores: Sakowski,Sebastian; Krasinski,Tadeusz; Waldmajer,Jacek; Sarnik,Joanna; Blasiak,Janusz; Poplawski,Tomasz.
Abstract The development of conventional, silicon-based computers has several limitations, including some related to the Heisenberg uncertainty principle and the von Neumann “bottleneck”. Biomolecular computers based on DNA and proteins are largely free of these disadvantages and, along with quantum computers, are reasonable alternatives to their conventional counterparts in some applications. The idea of a DNA computer proposed by Ehud Shapiro’s group at the Weizmann Institute of Science was developed using one restriction enzyme as hardware and DNA fragments (the transition molecules) as software and input/output signals. This computer represented a two-state two-symbol finite automaton that was subsequently extended by using two restriction enzymes. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics; DNA; DNA computer; Restriction enzymes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500860

Waardenburg Syndrome: description of two novel mutations in the PAX3 gene, one of which incompletely penetrant
Provedor de dados: Genetics and Molecular Biology
Autores: Pardono,Eliete; Mazzeu,Juliana F.; Lezirovitz,Karina; Auricchio,Maria Teresa B.M.; Iughetti,Paula; Nascimento,Rafaella M.P.; Mingroni-Netto,Regina C.; Otto,Paulo A.
We describe two different novel mutations in the PAX3 gene, detected in two families with cases of Waardenburg syndrome type I (WSI). The missense mutation detected in one family involved a single substitution in exon 2 (c.142 G &gt; T) and was present both in the affected individual and in his clinically normal father. The mutation found in the second family consisted of a deletion of 13 bases, c.764-776del(TTACCCTGACATT), in exon 5.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Waardenburg syndrome; PAX3 gene; Incomplete penetrance; Sensorineural hearing impairment; Telecanthus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400003

Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays
Provedor de dados: Genetics and Molecular Biology
Autores: Dourado,Priscila Leocádia Rosa; Rocha,Monyque Palagano da; Roveda,Liriana Mara; Raposo Junior,Jorge Luiz; Cândido,Liliam Sílvia; Cardoso,Claudia Andréa Lima; Morales,Maria Aparecida Marin; Oliveira,Kelly Mari Pires de; Grisolia,Alexeia Barufatti.
Abstract This study aimed to evaluate DNA damage in animal and plant cells exposed to water from the Água Boa stream (Dourados, Mato Grosso do Sul, Brazil) by using bioassays, and to identify the chemical compounds in the water to determine the water quality in the area. Through the cytotoxicity bioassay with Allium cepa, using micronucleus test, and comet assay, using Astyanax altiparanae fish, the results indicated that biological samples were genetically altered. Micronuclei were observed in erythrocytes of A. altiparanae after exposure to water from locations close to industrial waste discharge. The highest DNA damage observed with the comet assay in fish occurred with the exposure to water from locations where the presence of metals (Cu, Pb, Cd, Ni)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Astyanax altiparanae; Mutagenicity; Cytotoxicity; Genotoxicity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100123

k-Casein, b-lactoglobulin and growth hormone allele frequencies and genetic distances in Nelore, Gyr, Guzerá, Caracu, Charolais, Canchim and Santa Gertrudis cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Kemenes,Paola Augusta; Regitano,Luciana Correia de Almeida; Rosa,Artur Jordão de Magalhães; Packer,Irineu Umberto; Razook,Alexander George; Figueiredo,Leopoldo Andrade de; Silva,Nirlei Aparecida; Etchegaray,Maria Antônia L.; Coutinho,Luiz Lehmann.
The genotypes for k-casein (&lt;FONT FACE="Symbol"&gt;k&lt;/FONT&gt;-CN), &lt;FONT FACE="Symbol"&gt;b&lt;/FONT&gt;-lactoglobulin (&lt;FONT FACE="Symbol"&gt;b&lt;/FONT&gt;-LG) and growth hormone (GH) were determined by polymerase chain reaction (PCR) and restriction enzyme digestion in seven breeds of cattle (Nelore, Gyr, Guzerá, Caracu, Charolais, Canchim and Santa Gertrudis). &lt;FONT FACE="Symbol"&gt;k&lt;/FONT&gt;-Casein had two alleles with the A allele occurring at a higher frequency in Bos indicus breeds (0.93, 0.92 and 0.91% for Gyr, Guzerá and Nelore, respectively). The &lt;FONT FACE="Symbol"&gt;b&lt;/FONT&gt;-lactoglobulin locus had two alleles in all of the breeds. European breeds had a higher frequency of the &lt;FONT...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400012

Altered expression of the caffeine synthase gene in a naturally caffeine-free mutant of Coffea arabica
Provedor de dados: Genetics and Molecular Biology
Autores: Maluf,Mirian Perez; Silva,Carla Cristina da; Oliveira,Michelle de Paula Abreu de; Tavares,Aline Gomes; Silvarolla,Maria Bernadete; Guerreiro Filho,Oliveiro.
In this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contained an extra transcript of the caffeine synthase gene. This extra transcript contained only part of exon 1 and all of exon 3. The sequence of the mutant caffeine synthase gene revealed the substitution of isoleucine for valine in the enzyme active site that probably interfered with enzymatic activity. These findings indicate that the absence of caffeine in these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Cup quality; Differential expression; Naturally decaffeinated.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400023

Terpene production in the peel of sweet orange fruits
Provedor de dados: Genetics and Molecular Biology
Autores: Takita,Marco A.; Berger,Irving J.; Basílio-Palmieri,Ana Carolina; Borges,Kleber M.; Souza,Juliana M. de; Targon,Maria L.N.P.
Terpenoids constitute the largest and most diverse class of natural products. They are important factors for aroma and flavor, and their synthesis is basically done from two compounds: isopentenyl diphosphate and dimethylallyl diphosphate. Isopentenyl diphosphate is synthesized through two different pathways, one that occurs in the cytoplasm and one in the plastid. With the sequencing of ESTs from citrus, we were able to perform in silico analyses on the pathways that lead to the synthesis of terpenes as well as on the terpene synthases present in sweet orange. Moreover, expression analysis using real-time qPCR was performed to verify the expression pattern of a terpene synthase in plants. The results show that all the pathways for isopentenyl diphosphate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EST; Fruit; Terpenoids; Orange; Essential oil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500012

Genetic control of soybean (Glycine max) yield in the absence and presence of the Asian rust fungus (Phakopsora pachyrhizi)
Provedor de dados: Genetics and Molecular Biology
Autores: Ribeiro,Aliny Simony; Toledo,José Francisco Ferraz de; Arias,Carlos Alberto Arrabal; Godoy,Cláudia Vieira; Soares,Rafael Moreira; Moreira,José Ubirajara Vieira; Pierozzi,Pedro Henrique Braga; Vidigal,Maria Celeste Gonçalves; Oliveira,Marcelo Fernandes de.
Soybean is one of the most important crops in Brazil and continuously generates demands for production technologies, such as cultivars resistant to diseases. In recent years, the Asian rust fungus (Phakopsora pachyrhizi Syd. &amp;amp; P. Syd 1914) has caused severe yield losses and the development of resistant cultivars is the best means of control. Understanding the genetic control and estimating parameters associated with soybean (Glycine max) resistance to P. pachyrhizi will provide essential information for cultivar selection. We investigated quantitative genetic control of P. pachyrhizi and estimated parameters associated to soybean yield in the absence and presence of this phytopathogen. Six cultivars and their 15 diallel derived F2 and F3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic components; Genetic potential; Yield prediction.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100018

Bovine kappa-casein gene polymorphism and its association with milk production traits
Provedor de dados: Genetics and Molecular Biology
Autores: Rachagani,Satyanarayana; Gupta,Ishwar Dayal.
Point mutations in exon IV of the bovine κ-casein (CSN3) gene determine two allelic variants, A and B. These variants were distinguished by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis in the indigenous Sahiwal and Tharparkar cattle breeds. DNA samples (252 Sahiwal and 56 Tharparkar) were analyzed for allelic variants of the CSN3 gene. Polymorphism was detected by digestion of PCR-amplified products with HindIII, HhaI and HaeIII restriction enzymes, followed by separation on 3% agarose gels, and resolved by ethidium bromide staining. Allele A of the κ-casein gene occurred at a higher frequency than allele B, in both Sahiwal and Tharparkar breeds. The genotypic frequencies of AA, AB, and BB in the Sahiwal and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indian cattle; Κ-casein; Genetic polymorphism; PCR-RFLP.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500015

E6, a dominant gene conditioning early flowering and maturity in soybeans
Provedor de dados: Genetics and Molecular Biology
Autores: Bonato,Emídio Rizzo; Vello,Natal Antonio.
Inheritance was studied in natural variants of the soybean cultivar Paraná, developed under photoperiodic conditions ranging from 13 h 31 min day light, at sowing, to 14 h 23 min, 59 days afterwards. Results indicated that early flowering and maturity are controlled by a single dominant gene. Natural mutations that originated cultivars Paranagoiana and SS-1 occurred at the same locus of cultivar Paraná. It was not possible to determine if the recessive alleles of these mutant cultivars are different. The designation E6 was proposed for the alleles determining earliness in cultivar Paraná, and e6 for the gene determining late flowering and maturity in cultivars Paranagoiana and SS-1, until the individuality of the alleles of Paranagoiana and SS-1 is...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200016

Polymorphisms of the DNA repair genes XRCC1 and XRCC3 in a Brazilian population
Provedor de dados: Genetics and Molecular Biology
Autores: Duarte,Márcia Cristina; Colombo,Jucimara; Rossit,Andréa Regina Baptista; Silva,Ana Elizabete.
In several DNA repair genes, polymorphisms may result in reduced repair capacity, which has been implicated as a risk factor for various types of cancer. The frequency of the polymorphic alleles varies among populations, suggesting an ethnic distribution of genotypes. We genotyped 300 healthy Southeastern Brazilian individuals (262 of European ancestry and 38 of African ancestry) for polymorphisms of codons 194 and 399 of the XRCC1 base excision repair pathway gene and of codon 241 of the XRCC3 homologous recombination repair pathway gene. The allele frequencies were 0.07 for the Arg194Trp and 0.33 for the Arg399Gln codons of the XRCC1 gene and 0.35 for the Thr241Met codon of the XRCC3 gene. The genotypic frequencies were within Hardy-Weinberg equilibrium....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: DNA repair; XRCC1; XRCC3; Polymorphism; Ethnic variability.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300011

Identification of a novel mutation in ARSA gene in three patients of an Iranian family with metachromatic leukodystrophy disorder
Provedor de dados: Genetics and Molecular Biology
Autores: Golchin,Neda; Hajjari,Mohammadreza; Malamiri,Reza Azizi; Aminzadeh,Majid; Mohammadi-asl,Javad.
Abstract Metachromatic leukodystrophy disorder (MLD) is an autosomal recessive and lysosomal storage disease. The disease is caused by the deficiency of the enzyme arylsulfatase A (ARSA) which is encoded by the ARSA gene. Different mutations have been reported in different populations. The present study was aimed to detect the mutation type of the ARSA gene in three relative Iranian patients. We found a novel homozygous missense mutation c.1070 G &gt; T (p.Gly357Val) in exon 6 of these patients. The mutation was found to be reported for the first time in MLD patients. The data can update the mutation profile and contribute toward improved clinical management and counseling of MLD patients.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metachromatic leukodystrophy disorder; ARSA gene; Mutation; Arylsulfatase A.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500759

A critical evaluation of the effect of population size and phenotypic measurement on QTL detection and localization using a large F2 murine mapping population
Provedor de dados: Genetics and Molecular Biology
Autores: Li,Xinmin; Quigg,Richard J; Zhou,Jian; Xu,Shizhong; Masinde,Godfred; Mohan,Subburaman; Baylink,David J.
Population size and phenotypic measurement are two key factors determining the detection power of quantitative trait loci (QTL) mapping. We evaluated how these two controllable factors quantitatively affect the detection of QTL and their localization using a large F2 murine mapping population and found that three main points emerged from this study. One finding was that the sensitivity of QTL detection significantly decreased as the population size decreased. The decrease in the percentage logarithm of the odd score (LOD score, which is a statistical measure of the likelihood of two loci being lied near each other on a chromosome) can be estimated using the formula 1 - n/N, where n is the smaller and N the larger population size. This empirical formula has...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: QTL mapping; Mice; Detection power; Population size; Phenotypic measurement.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100030

Olive oil protects against chromosomal aberrations induced by doxorubicin in wistar rat bone marrow cells
Provedor de dados: Genetics and Molecular Biology
Autores: Antunes,Lusânia Maria Greggi; Takahashi,Catarina Satie.
There is considerable interest in identifying dietary compounds which have the capacity to protect against chromosomal aberrations induced by antitumor agents. Fatty acids and their constituents are able to act as free radical scavengers. Doxorubicin (DXR) is an important chemotherapeutic agent, that also induces chromosome aberrations. Rat bone marrow cells treated simultaneously with olive oil (10 ml/kg body weight) and DXR (90 mg/kg body weight) developed significantly fewer chromosomal aberrations and abnormal metaphases than those treated with DXR alone.
Tipo: Info:eu-repo/semantics/other
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200015

Array-CGH testing in spontaneous abortions with normal karyotypes
Provedor de dados: Genetics and Molecular Biology
Autores: Borovik,Cleide L.; Perez,Ana Beatriz A.; Silva,Luciana R.J. da; Krepischi-Santos,Ana Cristina V.; Costa,Silvia S.; Rosenberg,Carla.
In about 50% of first trimester spontaneous abortion the cause remains undetermined after standard cytogenetic investigation. We evaluated the usefulness of array-CGH in diagnosing chromosome abnormalities in products of conception from first trimester spontaneous abortions. Cell culture was carried out in short- and long-term cultures of 54 specimens and cytogenetic analysis was successful in 49 of them. Cytogenetic abnormalities (numerical and structural) were detected in 22 (44.89%) specimens. Subsequent, array-CGH based on large insert clones spaced at ~1 Mb intervals over the whole genome was used in 17 cases with normal G-banding karyotype. This revealed chromosome aneuplodies in three additional cases, giving a final total of 51% cases in which an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spontaneous abortion; Chromosomal aberrations; Array-CGH.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300004

Genetic identification of bucktooth parrotfish Sparisoma radians (Valenciennes, 1840) (Labridae, Scarinae) by chromosomal and molecular markers
Provedor de dados: Genetics and Molecular Biology
Autores: Paim,Fabilene Gomes; Brandão,José Henrique Souza Galdino; Sampaio,Iracilda; Affonso,Paulo Roberto Antunes de Mello; Diniz,Débora.
Parrotfishes (Labridae, Scarinae) comprise a large marine fish group of difficult identification, particularly during juvenile phase when the typical morphology and coloration of adults are absent. Therefore, the goal of this study was to test cytogenetic markers and DNA barcoding in the identification of bucktooth parrtotfish Sparisoma radians from the northeastern coast of Brazil. Sequencing of cytochrome c oxidase subunit I (COI) confirmed all studied samples as S. radians, and all showed high similarity (99-100%) with Caribbean populations. The karyotype of this species was divergent from most marine Perciformes, being composed of 2n = 46 chromosomes. These consisted of a large number of metacentric and submetacentric pairs with small amounts of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COI; Karyotype; South Atlantic fish; Ribossomal genes; Robertsonian rearrangements.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500007

Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: a new insight into an old approach
Provedor de dados: Genetics and Molecular Biology
Autores: Rossi,Ciro César; Araújo,Elza Fernandes de; Queiroz,Marisa Vieira de; Bazzolli,Denise Mara Soares.
The OmlA protein is a virulence factor of Actinobacillus pleuropneumoniae, an important pathogen in pigs. The polymorphisms present in the omlA gene sequence of 15 reference serotypes of A. pleuropneumoniae and non-serotypable isolates were assessed to determine the possible evolutionary relationship among them and to validate the importance of this gene as a molecular marker for the characterization of this bacterium. Divergence among the 15 serotypes of A. pleuropneumoniae probably resulted initially from two major evolutionary events that led to subsequent differentiation into nine groups. This differentiation makes it possible to characterize most of the serotypes by using bionformatics, thereby avoiding problems with immunological cross-reactivity. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actinobacillus pleuropneumoniae; OmlA gene; Phylogenetic reconstruction; Porcine pleuropneumonia.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200015

Codominant inheritance of polymorphic color variants of Gracilaria domingensis (Gracilariales, Rhodophyta)
Provedor de dados: Genetics and Molecular Biology
Autores: Plastino,Estela M.; Guimarães,Melina; Matioli,Sergio R.; Oliveira,Eurico C.
Agar is the most valuable phycocoloid in the world market. Currently, about half of its production is obtained from the red alga Gracilaria (Gracilariales, Rhodophyta). Consequently, this genus has been the subject of many studies worldwide. A common green color variant of G. domingensis (Kützing) Sonder ex Dickie was found in a natural population on the northeastern coast of Brazil. Crosses were performed to determine the mode of color inheritance. The results can be interpreted as the expression of a pair of codominant alleles, where the green and red phenotypes are homozygous, and the heterozygotes present a brownish color. Heterozygous tetrasporophytes, at least until they are 4-5 cm long, exhibit a parental influence on the female gametophyte, since...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100020

Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
Provedor de dados: Genetics and Molecular Biology
Autores: Jamsari,Amirul Firdaus Jamaluddin; Min-Pau,Tan; Siti-Azizah,Mohd Nor.
Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.120 to 0.880 and 0.117 to 0.698, respectively. A single locus (CS1-C07) was significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These novel markers would be useful for population genetic studies of the C. striata.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Channa striata; Population genetics; Microsatellite; Multiplex PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200029

Selection and genetic gains for juvenile traits in progenies of Hevea in São Paulo State, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Paulo de Souza; Martins,Antonio Lúcio Mello; Bortoletto,Nelson; Saes,Luis Alberto.
Five yield traits were investigated in three-year-old progenies from open-pollinated rubber trees [Hevea brasiliensis (Willd. ex Adr. de Juss) Muell.-Arg.]. Twenty progenies were evaluated in a randomized, complete block design replicated three times using 10 plants per linear plot at the North Central Experimental Station in Pindorama, São Paulo State, Brazil. The characters evaluated included the average yield of rubber, growth vigor, bark thickness, total number of latex vessel rings and latex vessel size. Highly significant (p &lt; 0.01) genetic differences were observed among progenies for most traits. The genotypic variance components accounted for 13.2%, 12.3%, 9.4%, 3.4% and 0.23% of the phenotypic variance for yield, growth vigor, bark thickness,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hevea brasiliensis; Rubber tree; Heritability; Genetic parameters.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200014

Mapping oligogenes for atopy and asthma by meta-analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Collins,A.; Ennis,S.; Tapper,W.; Morton,N.E.
Meta-analysis is presented for published studies on linkage or allelic association that have in common only reported significance levels. Reporting is biassed, and nonsignificance is seldom quantified. Therefore meta-analysis cannot identify oligogenes within a candidate region nor establish their significance, but it defines candidate regions well. Applied to a database on atopy and asthma, candidate regions are identified on chromosomes 6, 5, 16, 11, 12, 13, 14, 7, 20, and 10, in rank order from strongest to weakest evidence. On the other hand, there is little support for chromosomes 9, 8, 18, 1, and 15 in the same rank order. The evidence from 156 publications is reviewed for each region. With reasonable type I and II errors several thousand affected...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100001

In silicio expression analysis of PKS genes isolated from Cannabis sativa L.
Provedor de dados: Genetics and Molecular Biology
Autores: Flores-Sanchez,Isvett J.; Linthorst,Huub J.M.; Verpoorte,Robert.
Cannabinoids, flavonoids, and stilbenoids have been identified in the annual dioecious plant Cannabis sativa L. Of these, the cannabinoids are the best known group of this plant's natural products. Polyketide synthases (PKSs) are responsible for the biosynthesis of diverse secondary metabolites, including flavonoids and stilbenoids. Biosynthetically, the cannabinoids are polyketide substituted with terpenoid moiety. Using an RT-PCR homology search, PKS cDNAs were isolated from cannabis plants. The deduced amino acid sequences showed 51%-73% identity to other CHS/STS type sequences of the PKS family. Further, phylogenetic analysis revealed that these PKS cDNAs grouped with other non-chalcone-producing PKSs. Homology modeling analysis of these cannabis PKSs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cannabis sativa; Homology modeling; Polyketide synthases; RT-PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400017

Gestational diabetes mellitus (GDM) decreases butyrylcholinesterase (BChE) activity and changes its relationship with lipids
Provedor de dados: Genetics and Molecular Biology
Autores: Guimarães,Larissa O.; Andrade,Fabiana A. de; Bono,Gleyse F.; Setoguchi,Thaís E.; Brandão,Mariana B.; Chautard-Freire-Maia,Eleidi A.; Santos,Izabella C.R. dos; Picheth,Geraldo; Faria,Ana Cristina R. de A.; Réa,Rosângela R.; Souza,Ricardo L.R.; Furtado-Alle,Lupe.
Many conditions interfere with butyrylcholinesterase (BChE) activity, e.g., pregnancy or presence of the BCHE gene variant -116A can decrease activity whereas obesity and types I and II diabetes mellitus can increase activity. In this study, we examined BChE activity, -116A and 1615A BCHE gene variants, and anthropometric and biochemical variables associated with diabetes in patients with gestational diabetes mellitus (GDM) and in healthy pregnant women. BChE activity was measured spectrophotometrically using propionylthiocholine as substrate and genotyping of the -116 and 1615 sites of the BCHE gene was done with a TaqMan SNP genotyping assay. Three groups were studied: 150 patients with GDM, 295 healthy pregnant women and 156 non-pregnant healthy women....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Butyrylcholinesterase (BChE); Gestational diabetes mellitus; -116A variant; 1615A variant.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100003

Analysis of the CCR5 gene coding region diversity in five South American populations reveals two new non-synonymous alleles in Amerindians and high CCR5*D32 frequency in Euro-Brazilians
Provedor de dados: Genetics and Molecular Biology
Autores: Boldt,Angelica B.W.; Culpi,Lodércio; Tsuneto,Luiza T.; Souza,Ilíada R.; Kun,Jürgen F.J.; Petzl-Erler,Maria Luiza.
The CC chemokine receptor 5 (CCR5) molecule is an important co-receptor for HIV. The effect of the CCR5*D32 allele in susceptibility to HIV infection and AIDS disease is well known. Other alleles than CCR5*D32 have not been analysed before, neither in Amerindians nor in the majority of the populations all over the world. We investigated the distribution of the CCR5 coding region alleles in South Brazil and noticed a high CCR5*D32 frequency in the Euro-Brazilian population of the Paraná State (9.3%), which is the highest thus far reported for Latin America. The D32 frequency is even higher among the Euro-Brazilian Mennonites (14.2%). This allele is uncommon in Afro-Brazilians (2.0%), rare in the Guarani Amerindians (0.4%) and absent in the Kaingang...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CCR5; Brazilian; Amerindian; HIV; Polymorphism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100002

Karyological characterization of four Neotropical fish species of the genus Hisonotus (Teleostei, Loricariidae, Hypoptopomatinae) from distinct Brazilian river basins
Provedor de dados: Genetics and Molecular Biology
Autores: Andreata,Artur Antonio; Oliveira,Claudio; Foresti,Fausto.
The karyotypes of four Hisonotus species (two provisionally-named species A and D, H. nigricauda, and H. leucofrenatus) were found to have the same diploid number of 2n = 54 and interstitial silver-staining nucleolus organizer regions (Ag-NORs) located on the long arm of the largest metacentric pair. The C-banding pattern appeared to be species-specific, with one group (H. nigricauda and the unnamed species A and D) being characterized by small amounts of positive C-banded segments and containing a sub-group (species A and D) identified by a large positive C-banded segment on a small metacentric chromosome pair. The second group contained different samples of H. leucofrenatus, characterized by a larger amount of C-band positive segments spread over several...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish cytogenetics; Karyotypes; Ag-NORs; C-band; Chromosome evolution.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100013

Genetic parameters for growth, reproductive and maternal traits in a multibreed meat sheep population
Provedor de dados: Genetics and Molecular Biology
Autores: Lôbo,Ana Maria Bezerra Oliveira; Lôbo,Raimundo Nonato Braga; Paiva,Samuel Rezende; Oliveira,Sônia Maria Pinheiro de; Facó,Olivardo.
The genetic parameters for growth, reproductive and maternal traits in a multibreed meat sheep population were estimated by applying the Average Information Restricted Maximum Likelihood method to an animal model. Data from a flock supported by the Programa de Melhoramento Genético de Caprinos e Ovinos de Corte (GENECOC) were used. The traits studied included birth weight (BW), weaning weight (WW), slaughter weight (SW), yearling weight (YW), weight gain from birth to weaning (GBW), weight gain from weaning to slaughter (GWS), weight gain from weaning to yearling (GWY), age at first lambing (AFL), lambing interval (LI), gestation length (GL), lambing date (LD - number of days between the start of breeding season and lambing), litter weight at birth (LWB)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Age at first lambing; Genetic correlation; Heritability; Body weight; Litter weight.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400016

Exploring the role of auxin in the androgynophore movement in Passiflora
Provedor de dados: Genetics and Molecular Biology
Autores: Scorza,Livia C.T.; Dornelas,Marcelo Carnier.
The flowers of the species belonging to the genus Passiflorashow a range of features that are thought to have arisen as adaptations to different pollinators. Some Passiflora species belonging to the subgenus Decaloba sect. Xerogona, show touch-sensitive motile androgynophores. We tested the role of auxin polar transport in the modulation of the androgynophore movement by applying auxin (IAA) or an inhibitor of auxin polar transport (NPA) in the flowers. We recorded the movement of the androgynophore during mechano-stimulation and analyzed the duration, speed, and the angle formed by the androgynophore before and after the movement, and found that both IAA and NPA increase the amplitude of the movement in P. sanguinolenta. We hypothesize that auxin might...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora; Androgynophore; IAA; NPA; Thigmotropism.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300301

Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene
Provedor de dados: Genetics and Molecular Biology
Autores: Coêlho,Mariza M.; Ferreira-Nozawa,Monica S.; Nozawa,Sérgio R.; Santos,André L.W.
Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences deposited in GenBank, only seven had produced identifiable fragments. Bacillus, Pantoea and two non-culturable samples were identified. Results obtained through sequence analysis revealed low genetic diversity across the isolates, even when analyzing different species and plant structures. This is the first report concerning the isolation and identification of endophytic bacteria in these plant species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolation; Sequencing; Bacillus; Pantoea.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400021

Schizophrenia is not associated with the ERBB3 gene in a Han Chinese population sample: results from case-control and family-based studies
Provedor de dados: Genetics and Molecular Biology
Autores: Li,Dawei; He,Guang; Xu,Yifeng; Duan,Yun; Gu,Niufan; Li,Xingwang; Shi,Yongyong; Qin,Wei; Feng,Guoyin; He,Lin.
ERBB3 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 3), encoding a receptor of neuregulin-1 (NRG1), has been considered a functional candidate gene for schizophrenia susceptibility. In order to investigate a relationship between ERBB3 gene and schizophrenia in the Chinese population, case-control and family-based studies were carried out in 470 cases matched by controls, and in 532 family trios. Our results failed to show any evidence of significant association between the ERBB3 rs2292238 polymorphism and schizophrenia.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schizophrenia; ERBB3 gene.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400011

Test-day milk yield as a selection criterion for dairy buffaloes (Bubalus bubalis Artiodactyla, Bovidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Tonhati,Humberto; Cerón-Muñoz,Mário Fernando; Oliveira,João Ademir de; El Faro,Lenira; Lima,André Luís Ferreira; Albuquerque,Lucia Galvão de.
Due to the great demand for buffalo milk by-products the interest in technical-scientific information about this species is increasing. Our objective was to propose selection criteria for milk yield in buffaloes based on total milk yield, 305-day milk yield (M305), and test-day milk yield. A total of 3,888 lactations from 1,630 Murrah (Bubalus bubalis) cows recorded between 1987 and 2001, from 10 herds in the State of São Paulo, Brazil, were analyzed. Covariance components were obtained using the restricted maximum likelihood method applied to a bivariate animal model. Additive genetic and permanent environmental effects were considered as random, and contemporary group and lactation order as fixed effects. The heritability estimates were 0.22 for total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic correlations; Genetic parameters; Heritability; Selection efficiency.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400012

DNA polymorphism of leptin gene in Bos indicus and Bos taurus cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Choudhary,Vivek; Kumar,Pushpendra; Bhattacharya,Tarun K.; Bhushan,Bharat; Sharma,Arjava.
Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle. We used the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique to screen for DNA polymorphisms of the leptin gene in 403 cattle belonging to various breeds of Bos indicus (Hariana, Sahiwal, Gir and Nimari cattle), Bos taurus (Holstein Friesian (HF) and Jersey cattle) as well as Bos taurus x Bos indicus crossbreds (½ HF x ½ Hariana). In all the cattle we amplified two regions of the leptin gene, a 522 bp fragment comprising the partial intron 2 and exon 3 and another 94 bp fragment consisting of part of exon 2. Digestion of 522 bp PCR products with the BsaAI restriction enzyme revealed three genotypes in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leptin gene; PCR-RFLP; Polymorphism; Bos indicus; Bos taurus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500014

Genotoxic evaluation of sodium nitroprusside in Aspergillus nidulans
Provedor de dados: Genetics and Molecular Biology
Autores: Chiuchetta,Simone Jurema Ruggeri; Castro-Prado,Marialba Avezum Alves de.
The exogenous nitric oxide donor, sodium nitroprusside, evaluated the recombinogenic potential of nitric oxide. Drug inhibited mycelial growth and conidiation in A757 Aspergillus nidulans master strain. Two heterozygous diploid strains, one wild (uvsH+//uvsH+) and the other defective to DNA repair (uvsH//uvsH) were used for recombinagenesis tests. Sodium nitroprusside recombinogenic effect was evaluated by the induction of homozygosis of recessive genes, originally present in heterozygous condition. Results show that sodium nitroprusside (40 muM, 80 muM and 160 muM) is effective in inducing mitotic crossing-over in diploid cells of A. nidulans.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Parasexual cycle; Homozygotization index; Conidiation; Mitotic recombination.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500023

The TP53 fertility network
Provedor de dados: Genetics and Molecular Biology
Autores: Paskulin,Diego d'Avila; Paixão-Côrtes,Vanessa Rodrigues; Hainaut,Pierre; Bortolini,Maria Cátira; Ashton-Prolla,Patricia.
The TP53 gene, first described in 1979, was identified as a tumor suppressor gene in 1989, when it became clear that its product, the p53 nuclear phosphoprotein, was frequently inactivated in many different forms of cancers. Nicknamed "guardian of the genome", TP53 occupies a central node in stress response networks. The p53 protein has a key role as transcription factor in limiting oncogenesis through several growth suppressive functions, such as initiating apoptosis, senescence, or cell cycle arrest. The p53 protein is directly inactivated in about 50% of all tumors as a result of somatic gene mutations or deletions, and over 80% of tumors demonstrate dysfunctional p53 signaling. Beyond the undeniable importance of p53 as a tumor suppressor, an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TP53; Fertility; P53 network.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600008

Expression pattern of drought stress marker genes in soybean roots under two water deficit systems
Provedor de dados: Genetics and Molecular Biology
Autores: Neves-Borges,Anna Cristina; Guimarães-Dias,Fábia; Cruz,Fernanda; Mesquita,Rosilene Oliveira; Nepomuceno,Alexandre Lima; Romano,Eduardo; Loureiro,Marcelo Ehlers; Grossi-de-Sá,Maria de Fátima; Alves-Ferreira,Márcio.
The study of tolerance mechanisms for drought stress in soybean is fundamental to the understanding and development of tolerant varieties. Using in silico analysis, four marker genes involved in the classical ABA-dependent and ABA-independent pathways of drought response were identified in the Glycine max genome in the present work. The expression profiles of the marker genes ERD1-like, GmaxRD20A-like, GmaxRD22-like and GmaxRD29B-like were investigated by qPCR in root samples of drought sensitive and tolerant soybean cultivars (BR 16 and Embrapa 48, respectively), submitted to water deficit conditions in hydroponic and pot-based systems. Among the four putative soybean homologs to Arabidopsis genes investigated herein, only GmaxRD29B-like was not regulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water deficit; Drought adaptation; Marker genes; Glycine max.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200003

Localization of ribosomal genes in three Pimelodus species (Siluriformes, Pimelodidae) of the São Francisco River: 5S genes as species markers and conservation of the 18S rDNA sites
Provedor de dados: Genetics and Molecular Biology
Autores: Garcia,Caroline; Moreira Filho,Orlando.
Pimelodidae is one of the most representative of Neotropical catfish families. However, these fish are still poorly studied in terms of cytogenetics, especially regarding the application of more accurate techniques such as the chromosomal localization of ribosomal genes. In the present work, fluorescent in situ hybridization with 5S and 18S rDNA probes was employed for rDNA site mapping in Pimelodus sp., P. fur and P. maculatus from the São Francisco River in the Três Marias municipality - MG. The results from the application of the 18S probe confirmed the previous data obtained by silver nitrate staining, identifying a simple nucleolar organizing region system for these species. However, the labeling results from the 5S rDNA probe demonstrated a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pimelodus; Paracentric inversion; 5S rDNA; 18S rDNA; Species marker.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200018

Protection against laryngeal and pharyngeal carcinoma: Heterozygous vs. homozygous deletions of GSTM1 and GSTT1
Provedor de dados: Genetics and Molecular Biology
Autores: Masood,Nosheen; Kayani,Mahmood Akhtar.
Deletions in GSTM1 and GSTT1 genes are considered to be a risk factor for cancer development but the exact location of these deletions in the genome was unknown. Three main objectives of the current study were to: (a) identify the boundaries of these deletions in the human genome, (b) screen homozygous (-/-) and heterozygous (+/-) deleted, as well as homozygous present (+/+) individuals using PCR assays, (c) detect associations of pharyngeal (PC) and laryngeal cancer (LC) with the respective genotypes. In total, 102 PC and 92 LC patients were screened and compared with 150 controls. PCR mapping and sequencing revealed a 6 kbp deletion for GSTM1 and a 9 kbp deletion for the GSTT1 gene. The mean age of PC cases was 48.1 (±16.7) years; for LC cases it was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GSTM1; GSTT1 heterozygous; Homozygous; Deletions; Cancer.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100001

Up-regulation of RIP1 and IPS-1 in chronic HBV infected patients
Provedor de dados: Genetics and Molecular Biology
Autores: Safari-Arababadi,Minoo; Modarressi,Mohammad Hossein; Arababadi,Mohammad Kazemi.
Abstract IPS-1 and RIP1 are the main downstream molecules of RIG1 and MDA5, as intracytoplasmic receptors, which are the main receptors involved in recognition of internal and external viral double-stranded RNA. In this project, mRNA levels of IPS-1 and RIP1 were investigated in the peripheral blood immune cells of chronic hepatitis B (CHB) patients. IPS-1 and RIP1 mRNA levels were measured in 60 CHB patients and 120 healthy subjects, using RT-qPCR technique. A significant increase in expression levels of IPS-1 and RIP1 was found in patients when compared to healthy individuals. There was no correlation between IPS-1 and RIP1expression levels with the serum levels of hepatitis B e-Antigen (HBeAg) and liver enzymes in patients. Based on the results, it...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Innate immunity; Hepatitis B; RIP1; IPS-1.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300337

Analyses of DNA from ancient bones of a pre-Columbian Cuban woman and a child
Provedor de dados: Genetics and Molecular Biology
Autores: Lleonart,Ricardo; Riego,Eileen; Rodríguez Suárez,Roberto; Travieso Ruiz,Rafael; de la Fuente,José.
Molecular anthropology has brought new possibilities into the study of ancient human populations. Amplification of chromosomal short tandem repeat (STR) loci and mitochondrial DNA (mtDNA) has been successfully employed in analyses of ancient bone material. Although several studies have reported on continental Amerindian populations, none have addressed the ancient populations inhabiting the Caribbean islands. We used STR and mtDNA analyses to study the skeletal remains of a Cuban Ciboney female adult holding an infant. Results showed that for the STR analyzed the skeletal remains shared common alleles, suggesting a relationship. Mitochondrial DNA analysis showed sequence identity, thus corroborating a possible mother-child relationship. The mtDNA sequence...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300001

Genetic structure in two northern muriqui populations (Brachyteles hypoxanthus, Primates, Atelidae) as inferred from fecal DNA
Provedor de dados: Genetics and Molecular Biology
Autores: Fagundes,Valéria; Paes,Marcela F.; Chaves,Paulo B.; Mendes,Sérgio L.; Possamai,Carla de B.; Boubli,Jean P.; Strier,Karen B.
We assessed the genetic diversity of two northern muriqui (Brachyteles hypoxanthus Primata, Atelidae) populations, the Feliciano Miguel Abdala population (FMA, n = 108) in the Brazilian state of Minas Gerais (19°44' S, 41°49' W) and the Santa Maria de Jetibá population (SMJ, n = 18) in the Brazilian state of Espírito Santo (20°01' S, 40°44' W). Fecal DNA was isolated and PCR-RFLP analysis used to analyze 2160 bp of mitochondrial DNA, made up of an 820 bp segment of the gene cytochrome c oxidase subunit 2 (cox2, EC 1.9.3.1), an 880 bp segment of the gene cytochrome b (cytb, EC 1.10.2.2) and 460 bp of the hypervariable segment of the mtDNA control region (HVRI). The cox2 and cytb sequences were monomorphic within and between populations whereas the HVRI...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Brachyteles; Conservation genetics; Fecal DNA; MtDNA; PCR-RFLP.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100028

Comparative transcriptome and potential antiviral signaling pathways analysis of the gills in the red swamp crayfish, Procambarus clarkii infected with White Spot Syndrome Virus (WSSV)
Provedor de dados: Genetics and Molecular Biology
Autores: Du,Zhi-Qiang; Jin,Yan-Hui.
Abstract Red swamp crayfish is an important model organism for research of the invertebrate innate immunity mechanism. Its excellent disease resistance against bacteria, fungi, and viruses is well-known. However, the antiviral mechanisms of crayfish remain unclear. In this study, we obtained high-quality sequence reads from normal and white spot syndrome virus (WSSV)-challenged crayfish gills. For group normal (GN), 39,390,280 high-quality clean reads were randomly assembled to produce 172,591 contigs; whereas, 34,011,488 high-quality clean reads were randomly assembled to produce 182,176 contigs for group WSSV-challenged (GW). After GO annotations analysis, a total of 35,539 (90.01%), 14,931 (37.82%), 28,221 (71.48%), 25,290 (64.05%), 15,595 (39.50%), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Procambarus clarkii; WSSV; Gills; Illumina sequencing; De novo assembly; Comparative transcriptomics.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100168

MicroRNAs play critical roles during plant development and in response to abiotic stresses
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Júlio César de; Loss-Morais,Guilherme; Margis,Rogerio.
MicroRNAs (miRNAs) have been identified as key molecules in regulatory networks. The fine-tuning role of miRNAs in addition to the regulatory role of transcription factors has shown that molecular events during development are tightly regulated. In addition, several miRNAs play crucial roles in the response to abiotic stress induced by drought, salinity, low temperatures, and metals such as aluminium. Interestingly, several miRNAs have overlapping roles with regard to development, stress responses, and nutrient homeostasis. Moreover, in response to the same abiotic stresses, different expression patterns for some conserved miRNA families among different plant species revealed different metabolic adjustments. The use of deep sequencing technologies for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiRNAs; Development; Abiotic stress; Nutrients; Deep sequencing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600023

Genetic divergence in mitochondrial DNA of Anopheles nuneztovari (Diptera: Culicidae) from Brazil and Colombia
Provedor de dados: Genetics and Molecular Biology
Autores: Scarpassa,Vera Margarete; Geurgas,Silvia; Azeredo-Espin,Ana Maria L.; Tadei,Wanderli Pedro.
In the present study, we have examined the variability in Anopheles nuneztovari mitochondrial DNA of three populations from the Brazilian Amazon and one from western Colombia (Sitronela), using four restriction endonucleases (BclI, ClaI, HindIII, SstI). The haplotype diversity (h) was slightly elevated in all populations (0.5000 to 0.6765), whereas the nucleotide diversity (pi) was lower in the Sitronela population (0.0029) and higher in populations from the Brazilian Amazon (0.0056 to 0.0098). The degree of sequence divergence (delta) estimated within the Brazilian Amazon and that in Sitronela (0.0329 to 0.0371) suggests that these geographic populations of A. nuneztovari may eventually constitute separate species. The low sequence divergence values among...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100013

Allelic frequencies and statistical data obtained from 12 codis STR loci in an admixed population of the Brazilian Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Francez,Pablo Abdon da Costa; Rodrigues,Elzemar Martins Ribeiro; Frazão,Gleycianne Furtado; Borges,Nathalia Danielly dos Reis; Santos,Sidney Emanuel Batista dos.
The allelic frequencies of 12 short tandem repeat loci were obtained from a sample of 307 unrelated individuals living in Macapá, a city in the northern Amazon region, Brazil. These loci are the most commonly used in forensics and paternity testing. Based on the allele frequency obtained for the population of Macapá, we estimated an interethnic admixture for the three parental groups (European, Native American and African) of, respectively, 46%, 35% and 19%. Comparing these allele frequencies with those of other Brazilian populations and of the Iberian Peninsula population, no significant distances were observed. The interpopulation genetic distances (F ST coefficients) to the present database ranged from F ST = 0.0016 between Macapá and Belém to F ST =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allelic frequencies; Brazilian Amazon population; Interethnic admixture.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100007

Mode of reproduction of Brazilian species of Adesmia (Leguminosae)
Provedor de dados: Genetics and Molecular Biology
Autores: Tedesco,Solange B.; Dall'Agnol,Miguel; Schifino-Wittmann,Maria Teresa; Valls,José F.M.
Mode of reproduction was studied in 15 species of Adesmia DC. (Leguminosae). In six species, three treatments were used: mutual pollination, mechanical stimulation and control. Fifty-four plants of these six species were grown in a greenhouse, individually isolated in nylon screen boxes. Flowers were labelled and submitted to the different treatments. In addition, the frequency of spontaneous self-pollination in the absence of pollinators was studied in 200 plants of nine other species. These 200 plants were kept in a greenhouse, which avoided contact with any possible pollinator. Adesmia bicolor, A. muricata, A. punctata and A. riograndensis produced seed both by cross- and self-pollination. Adesmia punctata and A. riograndensis need mechanical...
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200038

Genotoxicity assessment of a pharmaceutical effluent using four bioassays
Provedor de dados: Genetics and Molecular Biology
Autores: Bakare,Adekunle A.; Okunola,Alabi A.; Adetunji,Olusanmi A.; Jenmi,Hafeez B.
Pharmaceutical industries are among the major contributors to industrial waste. Their effluents when wrongly handled and disposed of endanger both human and environmental health. In this study, we investigated the potential genotoxicity of a pharmaceutical effluent, by using the Allium cepa, mouse- sperm morphology, bone marrow chromosome aberration (CA) and micronucleus (MN) assays. Some of the physico-chemical properties of the effluent were also determined. The A. cepa and the animal assays were respectively carried out at concentrations of 0.5, 1, 2.5, 5 and 10%; and 1, 5, 10, 25 and 50% of the effluent. There was a statistically different (p &lt; 0.05), concentration-dependent inhibition of onion root growth and mitotic index, and induction of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Pharmaceutical effluent; Mouse; Allium cepa; Chromosome; Spermatozoa; Micronucleus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200026

Expression of DLK1 and MEG3 genes in porcine tissues during postnatal development
Provedor de dados: Genetics and Molecular Biology
Autores: Oczkowicz,Maria; Piestrzyska-Kajtoch,Agata; Piórkowska,Katarzyna; Rejduch,Barbara; Rózycki,Marian.
The Drosophila-like homolog 1 (DLK1), a transmembrane signal protein similar to other members of the Notch/Delta/Serrate family, regulates the differentiation process in many types of mammalian cells. Callipyge sheep and DLK1 knockout mice are excellent examples of a fundamental role of the gene encoding DLK1 in muscle growth and fat deposition. DLK1 is located within co-regulated imprinted clusters (the DLK1/DIO3 domain), along with other imprinted genes. Some of these, e.g. the RNA coding MEG3 gene, presumedly interfere with DLK1 transcription. The aim of our study was to analyze DLK1 and MEG3 gene expression in porcine tissues (muscle, liver, kidney, heart, brain stem) during postnatal development. The highest expression of both DLK1 and MEG3 variant 1...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: DLK1; Imprinting; MEG3; Pigs; Polar overdominance.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400030

Genetic divergence among common bean cultivars from different races based on RAPD markers
Provedor de dados: Genetics and Molecular Biology
Autores: Duarte,Jair Moura; Santos,João Bosco dos; Melo,Leonardo Cunha.
The genetic divergence of 27 common bean (Phaseolus vulgaris L.) cultivars from different races was evaluated using RAPD markers. The association of this divergence with some indirect variability estimates obtained in field experiments was also assessed. Genetic distances were calculated using Sorensen-Dice's similarity coefficient, displayed in a dendrogram (UPGMA method), and by projection in two-dimensional space. Analysis of molecular variance (AMOVA) was used to evaluate the distribution of variance between and within domestication centers. RAPD marker genetic diversity within these centers was also calculated. The correlations between estimates of genetic distances obtained with RAPD markers and estimates of Mahalanobis' generalized distances,...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300023

The pgm locus and pigmentation phenotype in Yersinia pestis
Provedor de dados: Genetics and Molecular Biology
Autores: Leal-Balbino,Tereza Cristina; Leal,Nilma Cintra; Nascimento,Mirna Gisele Medeiros do; Oliveira,Maria Betânia Melo de; Balbino,Valdir de Queiroz; Almeida,Alzira Maria Paiva de.
The pigmentation (pgm) locus is a large unstable area of the Yersinia pestis chromosome composed of a segment of iron acquisition (HPI) linked to a pigmentation segment. In this work we examined the mobility of HPI and the pigmentation segment in three Y. pestis isolates using successive subcultures on Congo red agar (CRA) plates. Strain P. CE 882 was shown to be highly stable while strains P. Exu 340 and P. Peru 375 dissociated into several phenotypes, PCR analysis showing evidence of changes in the pgm locus of the derived cultures. Strains P. Exu 340 and P. Peru 375 produced previously unreported cultures positive for the pesticin/yersiniabactin outer membrane receptor (psn+) but negative for the iron-regulated protein (irp2-), suggesting the occurrence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Y. pestis; High pathogenicity island; Pgm; Psn.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100024

Computational analysis suggests that virulence of Chromobacterium violaceum might be linked to biofilm formation and poly-NAG biosynthesis
Provedor de dados: Genetics and Molecular Biology
Autores: Becker,Sidnei; Soares,Cíntia; Porto,Luismar Marques.
Groups of genes that produce exopolysaccharide with a N-acetyl-D-glucosamine monomer are in the genome of several pathogenic bacteria. Chromobacterium violaceum, an opportunistic pathogen, has the operon hmsHFR-CV2940, whose proteins can synthesize such polysaccharide. In this work, multiple alignments among proteins from bacteria that synthesize such polysaccharide were used to verify the existence of amino acids that might be critical for pathogen activity. Three-dimensional models were generated for spatial visualization of these amino acid residues. The analysis carried out showed that the protein HmsR preserves the amino acids D135, D228, Q264 and R267, considered critical for the formation of biofilms and, furthermore, that these amino acids are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; Exopolysaccharide; Chromobacterium violaceum pathogenicity; Comparative genomics.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300031

Trends in evolution of 5S rRNA of deuterostomes: bases and homogeneous clusters
Provedor de dados: Genetics and Molecular Biology
Autores: Subacius,Sandra Maria Rodrigues; Guimarães,Romeu Cardoso.
Evolution of metazoan 5S rRNA sequences was analyzed through base composition and types, location and frequency of clustered bases. Characters from sequences of protostomes did not show regular trends as compared with paleontology dating or organism complexity. Trends of increasing G and C, stronger in G clusters, and decreasing A and U, were detected in deuterostomes, in parallel with evolution of complexity. The multifunctional domain 71-104 was highlighted among conserved stretches. Clusters of C were typical of helices. Those of G were longer, extending from helices into loops or related to bulges, which is suggestive of functional significance. Deuterostomian trends were installed early in the lineage and reached full development in aquatic organisms,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ribosomal RNA; Evolution; Repetitive sites; Deuterostomes; Protostomes.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300006

Polymorphic heterologous microsatellite loci for population genetics studies of the white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Andiara Silos Moraes de Castro e; Miño,Carolina Isabel; Lama,Silvia Nassif Del.
We screened 44 heterologous microsatellites isolated in species of the families Threskiornithidae, Ciconiidae and Ardeidae for their use in a migratory waterbird, the white-faced ibis Plegadis chihi (Vieillot, 1817) (Threskiornithidae). Of the screened loci, 57% amplified successfully and 24% were polymorphic. In two breeding colonies from southern Brazil (N = 131) we detected 32 alleles (2-10 alleles/ locus). Average He over all loci and colonies was 0.55, and the combined probability of excluding false parents, 98%. There was no departure from HWE in any loci or population. Eru6 and Eru4 loci were in non-random association in the Alvorada colony, and NnNF5 and Eru5 in both populations. AMOVA analysis indicated that most of the genetic diversity was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cross-species amplification; Genetic diversity; Molecular markers; White-faced ibis; SSR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100010

Frequency and distribution of microsatellites from ESTs of citrus
Provedor de dados: Genetics and Molecular Biology
Autores: Palmieri,Darío Abel; Novelli,Valdenice Moreira; Bastianel,Marinês; Cristofani-Yaly,Mariângela; Astúa-Monge,Gustavo; Carlos,Eduardo Fermino; Oliveira,Antonio Carlos de; Machado,Marcos Antonio.
Nearly 65,000 citrus EST (Expressed Sequence Tags) have been investigated using the CitEST project database. Microsatellites were investigated in the unigene sequences from Citrus spp. and Poncirus trifoliata. From these sequences, approximately 35% of the non-redundant ESTs contained SSRs. The frequencies of different SSR motifs were similar between Citrus spp and trifoliate orange. In general, mononucleotide repeats appeared to be the most abundant SSRs in the CitEST database, but we also identify di-, tri-, tetra-, penta- and hexanucleotide repeats. The AG/CT and AAG/CTT were the most common dinucleotide and trinucleotide motifs, with frequencies of 54.4% and 25.2%, respectively. Primer sequences flanking SSR motifs were successfully designed and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus spp.; Poncirus trifoliata; ESTs; Microsatellites; In silico polymorphism.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500029

Novel insights into chromosome evolution of Charadriiformes: extensive genomic reshuffling in the wattled jacana (Jacana jacana, Charadriiformes, Jacanidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Kretschmer,Rafael; Souza,Marcelo Santos de; Barcellos,Suziane Alves; Degrandi,Tiago Marafiga; Pereira,Jorge C.; O’Brien,Patricia C.M.; Ferguson-Smith,Malcolm A.; Gunski,Ricardo José; Garnero,Analía del Valle; Oliveira,Edivaldo Herculano Correa de; Freitas,Thales Renato Ochotorena de.
Abstract The order Charadriiformes comprises three major clades: Lari and Scolopaci as sister group to Charadrii. Until now, only three Charadriiformes species have been studied by chromosome painting: Larus argentatus (Lari), Burhinus oedicnemus and Vanellus chilensis (Charadrii). Hence, there is a lack of information concerning the third clade, Scolapaci. Based on this, and to gain a better understanding of karyotype evolution in the order Charadriiformes, we applied conventional and molecular cytogenetic approaches in a species belonging to clade Scolopaci - the wattled jacana (Jacana jacana) - using Gallus gallus and Zenaida auriculata chromosome-specific probes. Cross-species evaluation of J. jacana chromosomes shows extensive genomic reshuffling...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Charadrii; Karyotype; Avian genome; Comparative mapping.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100601

Karyotype and nuclear DNA content of hexa-, octo-, and duodecaploid lines of Bromus subgen. Ceratochloa
Provedor de dados: Genetics and Molecular Biology
Autores: Klos,Joanna; Sliwinska,Elwira; Kula,Adam; Golczyk,Hieronim; Grabowska-Joachimiak,Aleksandra; Ilnicki,Tomasz; Szostek,Krzysztof; Stewart,Alan; Joachimiak,Andrzej J.
The subgenus Ceratochloa of the genus Bromus includes a number of closely related allopolyploid forms or species that present a difficult taxonomic problem. The present work combines data concerning chromosome length, heterochromatin distribution and nuclear genome size of different 6x, 8x and 12x accessions in this subgenus. Special attention is paid to the karyotype structure and genomic constitution of duodecaploid plants recently found in South America. Hexaploid lineages possess six almost indistinguishable genomes and a nuclear DNA content between 12.72 pg and 15.10 pg (mean 1Cx value = 2.32 pg), whereas octoploid lineages contain the same six genomes (AABBCC) plus two that are characterized by longer chromosomes and a greater DNA content (1Cx = 4.47...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; C-banding; Heterochromatin; Flow cytometry; Genome size.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300016

Genetic parameters for milk, fat and protein yields in Murrah buffaloes (Bubalus bubalis Artiodactyla, Bovidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Aspilcueta-Borquis,Rusbel Raúl; Sesana,Roberta Cristina; Berrocal,Milthon Honorio Munoz; Seno,Leonardo de Oliveira; Bignardi,Annaiza Braga; El Faro,Lenira; Albuquerque,Lucia Galvão de; Camargo,Gregório Miguel Ferreira de; Tonhati,Humberto.
The objective of the present study was to estimate genetic parameters for test-day milk, fat and protein yields and 305-day-yields in Murrah buffaloes. 4,757 complete lactations of Murrah buffaloes were analyzed. Co-variance components were estimated by the restricted maximum likelihood method. The models included additive direct genetic and permanent environmental effects as random effects, and the fixed effects of contemporary group, milking number and age of the cow at calving as linear and quadratic covariables. Contemporary groups were defined by herd-year-month of test for test-day yields and by herd-year-season of calving for 305-day yields. The heritability estimates obtained by two-trait analysis ranged from 0.15 to 0.24 for milk, 0.16 to 0.23 for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Test-day model; Accumulated productions; Heritability; Genetic correlations.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100014

Selection for later flowering in soybean (Glycine max L.Merrill) F2 populations cultivated under short day conditions
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Antonio Carlos Baião de; Sediyama,Carlos Sigueyuki; Cruz,Cosme Damião.
Several different selection strategies were used to estimate expected gain of days to flowering and other related characters in two F2 soybean (Glycine max (L.) Merrill) populations. The sample originated from crosses of lines whose seeds do not contain the three lipoxygenase isozymes with the commercially cultivated IAC-12. IAC-12 is a gene carrier for an extended juvenile period. This study was conducted during the winter of 1994 in Viçosa, Minas Gerais. The plants were grown under natural photoperiod. One population was grown in a heated greenhouse, and the other in the field under natural temperature conditions. Lower temperatures early in the field planting caused a delay in flowering. Delayed flowering caused a broader amplitude in all characters...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200019

XRCC1 gene polymorphisms in a population sample and in women with a family history of breast cancer from Rio de Janeiro (Brazil)
Provedor de dados: Genetics and Molecular Biology
Autores: Falagan-Lotsch,Priscila; Rodrigues,Marina S.; Esteves,Viviane; Vieira,Roberto; Amendola,Luis C.; Pagnoncelli,Dante; Paixão,Júlio C.; Gallo,Claudia V. De Moura.
The X-ray repair cross-complementing Group1 (XRCC1) gene has been defined as essential in the base excision repair (BER) and single-strand break repair processes. This gene is highly polymorphic, and the most extensively studied genetic changes are in exon 6 (Arg194Trp) and in exon 10 (Arg399Gln). These changes, in conserved protein sites, may alter the base excision repair capacity, increasing the susceptibility to adverse health conditions, including cancer. In the present study, we estimated the frequencies of the XRCC1 gene polymorphisms Arg194Trp and Arg399Gln in healthy individuals and also in women at risk of breast cancer due to family history from Rio de Janeiro. The common genotypes in both positions (194 and 399) were the most frequent in this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: XRCC1 gene polymorphisms; Breast cancer susceptibility; Brazilian population.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200008

Overexpression of rice F-box protein OsFBX322 confers increased sensitivity to gamma irradiation in Arabidopsis
Provedor de dados: Genetics and Molecular Biology
Autores: Hwang,Jung Eun; Hwang,Sun-Goo; Jung,In Jung; Han,Sung Min; Ahn,Joon-Woo; Kim,Jin-Baek.
Abstract Ionizing radiation has a substantial effect on physiological and biochemical processes in plants via induction of transcriptional changes and diverse genetic alterations. Previous microarray analysis showed that rice OsFBX322, which encodes a rice F-box protein, was downregulated in response to three types of ionizing radiation: gamma irradiation, ion beams, and cosmic rays. In order to characterize the radiation-responsive genes in rice, OsFBX322 was selected for further analysis. OsFBX322 expression patterns in response to radiation were confirmed using quantitative RT-PCR. Transient expression of a GFP-OsFBX322 fusion protein in tobacco leaf epidermis indicated that OsFBX322 is localized to the nucleus. To determine the effect of OsFBX322...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: F-box protein; Gamma-irradiation; Ionizing radiation; Rice; Transgenic plant.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100304

Karyology of the Atlantic forest rodent Juliomys (Cricetidae): a new karyotype from southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Paresque,Roberta; Christoff,Alexandre Uarth; Fagundes,Valéria.
Juliomys is a small rodent from the family Cricetidae which inhabits the Atlantic forest and forests from Argentina to eastern Brazil. The three species recognized so far have different karyotypes. In this paper, we describe a new karyotype with 2n = 32, FN = 48 found in Juliomys specimens from a high-altitude area in the Atlantic forest of southern Brazil. The karyotype was analyzed after G- and C-banding and silver staining of the nucleolus organizer regions (Ag-NOR) and its G-banding patterns were compared with those of the newly described species Juliomys ossitenuis (2n = 20, FN = 36). The 2n = 32 karyomorph presented peculiar features and was very different from those of the other species of the genus: J. pictipes (2n = 36, FN = 34), J. rimofrons (2n...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Juliomys; Karyotype evolution; J. ossitenuis; Comparative G-banding; Ag-NORs.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200016

Chromosomal localization of microsatellite loci in Drosophila mediopunctata
Provedor de dados: Genetics and Molecular Biology
Autores: Cavasini,Renato; Batista,Marcos Roberto Dias; Klaczko,Louis Bernard.
Drosophila mediopunctata has been used as a model organism for genetics and evolutionary studies in the last three decades. A linkage map with 48 microsatellite loci recently published for this species showed five syntenic groups, which had their homology determined to Drosophila melanogaster chromosomes. Then, by inference, each of the groups was associated with one of the five major chromosomes of D. mediopunctata. Our objective was to carry out a genetic (chromosomal) analysis to increase the number of available loci with known chromosomal location. We made a simultaneous analysis of visible mutant phenotypes and microsatellite genotypes in a backcross of a standard strain and a mutant strain, which had each major autosome marked. Hence, we could...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Tripunctata; SSR; Genetic chromosomal analysis; BLAST search; Muller’s elements.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100055

B-chromosomes in two Brazilian populations of Dendropsophus nanus (Anura, Hylidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Medeiros,Lilian R.; Rossa-Feres,Denise C.; Jim,Jorge; Recco-Pimentel,Shirlei M.
We report on the presence of B-chromosomes in two populations of Dendropsophus nanus (= Hyla nana Boulenger, 1889) from São Paulo State, Brazil. Such chromosomes were observed in 4 out of 43 specimens (9.3%) and in 9 out of 15 specimens (60%) from the municipalities of Nova Aliança and Botucatu, respectively. The karyotype 2n = 30 + 1B found in D. nanus was similar to that of other species with 2n = 30 chromosomes, except for the presence of an additional small telocentric chromosome. In one specimen from Botucatu, cells with one to three extra chromosomes were observed. These B-chromosomes appeared as univalent in meiosis I and did not bear a nucleolar organizer region or exhibit constitutive heterochromatin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anura; B-chromosome cytogenetics Dendropsophus supernumerary chromosome.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200010

Identification of Astyanax altiparanae (Teleostei, Characidae) in the Iguaçu River, Brazil, based on mitochondrial DNA and RAPD markers
Provedor de dados: Genetics and Molecular Biology
Autores: Prioli,Sônia M.A.P.; Prioli,Alberto J.; Júlio Jr.,Horácio F.; Pavanelli,Carla S.; Oliveira,Alessandra V. de; Carrer,Helaine; Carraro,Dirce M.; Prioli,Laudenir M.
Astyanax fishes are among the most important food-web components of South America rivers. In the Iguaçu River basin, the Astyanax genus is represented mainly by endemic species. For millions of years, that hydrographic basin has been geographically isolated from the Paraná River basin by the Iguaçu Falls. Recently, a species from the Upper Paraná River basin identified as Astyanax bimaculatus was revised and described as a new species named Astyanax altiparanae Garutti &amp;amp; Britski, 2000. Fauna endemism and geographic isolation triggered interest in investigations to evaluate the identification and genetic relatedness among two A. altiparanae populations from the Upper Paraná River basin and the population identified as A. bimaculatus in the Iguaçu...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astyanax; Fish; D-loop; Control region; Mitochondrial DNA; RAPD.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400011

Comparative mitogenomic analyses of Amazona parrots and Psittaciformes
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Nicholas Costa Barroso; Soares,André Elias Rodrigues; Almeida,Luiz Gonzaga de Paula; Costa,Igor Rodrigues da; Sato,Fernanda Midori; Schneider,Patricia; Aleixo,Alexandre; Schneider,Maria Paula; Santos,Fabrício R.; Mello,Claudio V.; Miyaki,Cristina; Vasconcelos,Ana Tereza R.; Prosdocimi,Francisco.
Abstract Amazon parrots are long-lived birds with highly developed cognitive skills, including vocal learning. Several parrot mitogenomes have been sequenced, but important aspects of their organization and evolution are not fully understood or have limited experimental support. The main aim of the present study was to describe the mitogenome of the blue-fronted Amazon, Amazona aestiva, and compare it to other mitogenomes from the genus Amazona and the order Psittaciformes. We observed that mitogenomes are highly conserved among Amazon parrots, and a detailed analysis of their duplicated control regions revealed conserved blocks. Population level analyses indicated that the specimen analyzed here seems to be close to A. aestiva individuals from Bahia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Next-generation-sequencing; Psittacidae; Mitogenomics; Control region.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400593

Performance of the Gail and Tyrer-Cuzick breast cancer risk assessment models in women screened in a primary care setting with the FHS-7 questionnaire
Provedor de dados: Genetics and Molecular Biology
Autores: Vianna,Fernanda Sales Luiz; Giacomazzi,Juliana; Oliveira Netto,Cristina Brinckmann; Nunes,Luciana Neves; Caleffi,Maira; Ashton-Prolla,Patricia; Camey,Suzi Alves.
Abstract Breast cancer (BC) risk assessment models base their estimations on different aspects of a woman’s personal and familial history. The Gail and Tyrer–Cuzick models are the most commonly used, and BC risks assigned by them vary considerably especially concerning familial history. In this study, our aim was to compare the Gail and Tyrer-Cuzick models after initial screening for familial history of cancer in primary care using the FHS-7 questionnaire. We compared 846 unrelated women with at least one positive answer to any of the seven FHS-7 questions (positive group) and 892 unrelated women that answered negatively (negative group). Concordance between BC risk estimates was compared by Bland-Altman graphics. Mean BC risk estimates were higher using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast neoplasms; Risk assessments; Family medical history; Hereditary breast and ovarian cancer.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200232

Margarete Suñé Mattevi (1941-2013) and the dynamics of chromosome changes
Provedor de dados: Genetics and Molecular Biology
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300001

Effects of Phaseolus vulgaris QTL in controlling host-bacteria interactions under two levels of nitrogen fertilization
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Alessandra A.; Boscariol,Raquel L.; Moon,David H.; Camargo,Luis E.A.; Tsai,Siu M.
Molecular markers were used to estimate the effect of mineral nitrogen on the phenotypic expression of quantitative trait loci (QTL) controlling the number of Rhizobium nodules (NN) and resistance to Xanthomonas axonopodis pv. phaseoli in the common bean. Recombinant inbred lines derived from a BAT-93 x Jalo EEP558 cross were grown in a greenhouse in the absence or presence (5 mM NH4NO3) of nitrogen. Resistance to Xanthomonas was assessed as diseased leaf area (DLA) and the number of nodules was obtained by direct counting. Analyses of variance were used to detect significant associations between 85 marker loci from 12 linkage groups (LG) and quantitative traits. In the absence of nitrogen, 15 and 11 markers, distributed over 7 and 5 LG, showed a...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100029

Suitability of DNA extracted from archival specimens of fruit-eating bats of the genus Artibeus (Chiroptera, Phyllostomidae) for polymerase chain reaction and sequencing analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Scatena,Mário Pinzan; Morielle-Versute,Eliana.
To establish a technique which minimized the effects of fixation on the extraction of DNA from formalin-fixed tissues preserved in scientific collections we extracted DNA samples from fixed tissues using different methods and evaluated the effect of the different procedures on PCR and sequencing analysis. We investigated muscle and liver tissues from museum specimens of five species of fruit-eating (frugivorous) bats of the Neotropical genus Artibeus (Chiroptera, Phyllostomidae): A. fimbriatus, A. lituratus, A. jamaicensis, A. obscurus, and A. planirostris. The results indicated that treatment of tissues in buffered solutions at neutral pH and about 37 °C for at least four days improves the quality and quantity of extracted DNA and the quality of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Archival specimens; Chiroptera; DNA extraction; Formalin-fixed.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100027

Cytochrome P450 (CYP) and glutathione S-transferases (GST) polymorphisms (CYP1A1, CYP1B1, GSTM1, GSTP1 and GSTT1) and urinary levels of 1-hydroxypyrene in Turkish coke oven workers
Provedor de dados: Genetics and Molecular Biology
Autores: Ada,Ahmet Oguz; Yilmazer,Meltem; Suzen,Sinan; Demiroglu,Canan; Demirbag,Ali Eba; Efe,Sibel; Alemdar,Yavuz; Burgaz,Sema; Iscan,Mumtaz.
Genetic polymorphisms of xenobiotic metabolizing enzymes have been associated with cancer risk. We evaluated the influences of genetic polymorphisms of polycyclic aromatic hydrocarbon (PAH) metabolizing enzymes on urinary 1-hydroxypyrene (1-OHP) excretion in Turkish coke oven workers. Urinary 1-OHP was analyzed by HPLC after enzymatic hydrolysis. Lymphocyte DNA was used for PCR-based genotyping of cytochrome P450 (CYP) polymorphisms (CYP1A1 and CYP1B1) and glutathione S-transferases (GST) polymorphisms (GSTM1, GSTT1 and GSTP1). The mean urinary 1-OHP levels of coke oven workers were significantly higher than that of controls. No significant difference was detected in the mean urinary 1-OHP levels of smokers and non-smokers either for coke oven workers or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polycyclic aromatic hydrocarbons; 1-hydroxypyrene; Coke oven workers; Cytochrome P450s; Glutathione S-transferases.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400002

Association of serum lipid components and obesity with genetic ancestry in an admixed population of elderly women
Provedor de dados: Genetics and Molecular Biology
Autores: Lins,Tulio C.; Pires,Alause S.; Paula,Roberta S.; Moraes,Clayton F.; Vieira,Rodrigo G.; Vianna,Lucy G.; Nobrega,Otávio T.; Pereira,Rinaldo W.
The prevalence of metabolic disorders varies among ethnic populations and these disorders represent a critical health care issue for elderly women. This study investigated the correlation between genetic ancestry and body composition, metabolic traits and clinical status in a sample of elderly women. Clinical, nutritional and anthropometric data were collected from 176 volunteers. Genetic ancestry was estimated using 23 ancestry-informative markers. Pearsons correlation test was used to examine the relationship between continuous variables and an independent samples t-test was used to compare the means of continuous traits within categorical variables. Overall ancestry was a combination of European (57.49%), Native American (25.78%) and African (16.73%)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dyslipidemia; Genetic ancestry; Lipid profile; Obesity; Postmenopausal women.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400005

Yield stability in maize (Zea mays L.) and correlations among the parameters of the Eberhart and Russell, Lin and Binns and Huehn models
Provedor de dados: Genetics and Molecular Biology
Autores: Scapim,Carlos Alberto; Oliveira,Valter Rodrigues; Braccini,Alessandro de Lucca e; Cruz,Cosme Damião; Andrade,Carlos Alberto de Bastos; Vidigal,Maria Celeste Gonçalves.
Assessment of the stability and adaptability of a genotype to different environments is useful for recommending cultivars for known conditions of cultivation and should be a requirement in breeding programs. Twenty maize (Zea mays L.) cultivars were tested at eight locations in Minas Gerais by the National Center for Maize and Sorghum Research (CNPMS) of the Brazilian Enterprise for Agricultural Research (EMBRAPA) for two years. The experiments involved a randomized complete block design in which three procedures were used to analyze cultivar stability and adaptability. The level of association among the parameters obtained by the three methods was assessed using Spearman's rank correlation. Hybrids 'DINA 170', 'G-96C', 'C 505', 'DINA 70' and 'C 435' had a...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200025

Distribution of the Bari-I transposable element in stable hybrid strains between Drosophila melanogaster and Drosophila simulans and in Brazilian populations of these species
Provedor de dados: Genetics and Molecular Biology
Autores: Lucca Jr.,Marcos de; Carareto,Claudia Marcia Aparecida; Ceron,Carlos Roberto.
We analyzed the distribution of the Bari-I transposable element in Drosophila melanogaster (IN(1)AB), its sibling species Drosophila simulans (C167.4) and in eight hybrid strains derived from initial crosses involving D. simulans females and D. melanogaster males of the above cited strains as well as in Brazilian populations of these species. Polymerase chain reaction (PCR) data showed the presence of the Bari-I element among species populations and hybrid strains. Hybridization with a 703 bp probe homologous to the Bari-I sequence showed that the number of Bari-I copies in D. melanogaster IN(1)AB was higher than in D. simulans C167.4 strains. Hybrid strains presented Bari-I sequences related to both parental species. In addition some strains displayed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bari-I; Drosophila melanogaster; D. simulans; Introgressive hybridization; Transposable element.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400028

Cytogenetic study of women with premature ovarian failure
Provedor de dados: Genetics and Molecular Biology
Autores: Pagni,Denise; Melaragno,Maria Isabel.
Etiology of premature ovarian failure (POF) is unclear in most patients. Since some cases are related to X-chromosome abnormalities, cytogenetical studies were conducted in patients with POF. Lymphocyte cultures from eleven patients were compared to cultures from age-matched controls. All individuals presented normal karyotypes. Frequencies of aneuploid and structural chromosome aberrations did not differ between the groups. The X chromosome was more frequently involved in aneuploidy and in premature centromere division in both groups.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200016

From abortion-inducing medications to Zika Virus Syndrome: 27 years experience of the First Teratogen Information Service in Latin America
Provedor de dados: Genetics and Molecular Biology
Autores: Schüler-Faccini,Lavinia; Sanseverino,Maria Teresa Vieira; Abeche,Alberto Mantovani; Vianna,Fernanda Sales Luiz; Fraga,Lucas Rosa; Rocha,Anastacia Guimaraes; Silva,André Anjos da; Souza,Paulo Ricardo Assis de; Hilgert,Artur Hartmann; Barbosa,Camila Pocharski; Kauppinem,Caroline Grasso; Martins,Daniela Fernandes; Santos,Daniela Silva; Colpes,Gabriel Henrique; Ecco,Gabriela; Silva,Helena Margot Flores Soares da; Penteado,Louise Piva; Santos,Tatiane dos.
Abstract In 1990, the first Teratogen Information Service in Brazil (SIAT) was implemented in the Medical Genetics Service at Hospital de Clinicas de Porto Alegre. SIAT is a free-to-use information service both to health professionals and the general population, especially to women who are pregnant or planning pregnancy. The main objective of this paper is to present the activities of SIAT in its initial years (1990-2006), compared to those in the last decade (2007-2017). In addition we review the scientific contribution of SIAT in the field of human teratogenesis. Since 1990, SIAT received 10,533 calls. Use of medications were the main reason for concern, accounting for 74% of all questions, followed by other chemical exposures (occupational, cosmetics,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Teratogens; Pregnancy; Zika; Thalidomide; Misoprostol; Rubella.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200297

Distribution and insertion numbers of transposable elements in species of the Drosophila saltans group
Provedor de dados: Genetics and Molecular Biology
Autores: Castro,Juliana P. de; Setta,Nathalia de; Carareto,Claudia Marcia A.
Information about the distribution and insertion numbers of many transposable elements is restricted to few species of Drosophila, although these elements are widely distributed throughout the genus. The aim of this work was to describe the distribution and insertion numbers of four retrotransposons (copia, gypsy, micropia, I) and four transposons (hobo, mariner, Minos and Bari-1) in the saltans group of Drosophila. Our data shows that, except for mariner, all the other elements are widespread within the saltans group and show variable insertion numbers of up to 24 copies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila; Genomic insertion; Saltans group; Retrotransposons; Transposons.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200029

A remarkable autosomal heteromorphism in Pseudoryzomys simplex 2n = 56; FN = 54-55 (Rodentia, Sigmodontinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Moreira,Camila Nascimento; Di-Nizo,Camilla Bruno; Silva,Maria José de Jesus; Yonenaga-Yassuda,Yatiyo; Ventura,Karen.
Pseudoryzomys simplex, the false rice rat, is a monotypic genus of the Oryzomyini tribe (Sigmodontinae) distributed in part of Bolivia, Paraguay, Argentina and Brazil. Its diploid number has been described as 56 acrocentric chromosomes decreasing in size and no karyotype figure has been depicted. Herein, we present karyotypic data on P. simplex, including chromosome banding and molecular fluorescent in situ hybridization using telomeric sequences and the whole X-chromosome of its sister clade Holochilus brasiliensis (HBR) as probes. A case of remarkable autosomal heteromorphism due to the presence of a whole heterochromatic arm leading to the variability of FN is reported, as well as the occurrence of regions of homology between the X and Y chromosomes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Fluorescent in situ hybridization; Heterochromatin; Oryzomyini.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200010

Selection and genetic gain in rubber tree (Hevea) populations using a mixed mating system
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Reginaldo Brito da; Resende,Marcos Deon Vilela de; Araujo,Antonio José de; Gonçalves,Paulo de Souza; Higa,Antonio Rioyei.
The components of genetic variation and genetic gain obtained with three selection methods - individual, combined and multi-effect index selection - were compared in rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell. Arg.] progenies. The rubber tree is a cross pollinating species with a mixed reproductive system in which the self pollination rate is 22%. Twenty-two half sib progenies were planted at experimental stations at Pindorama, Votuporanga and Jaú, in São Paulo State, using a randomized and complete block design, with five replications and ten plants per plot. Dry rubber production was assessed when the plants were three years old. Based on the genetic variability of the populations, Pindorama was the best environment for the...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300028

A hybrid zone of the genus Ctenomys: a case study in southern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Castilho,Camila S.; Gava,Adriana; Freitas,Thales R.O. de.
We describe variation at microsatellite loci and the chromosomal polymorphisms of a hybrid population, and hybridizing populations of Ctenomys minutus (the minor tuco-tuco) from the coastal plain of Rio Grande do Sul, southern Brazil. Cytogenetic analysis and a survey of six microsatellite loci included 101 specimens of C. minutus from the parental populations (2n/AN = 42/74 and 48a/76) and their contact zone. Cytogenetic analysis recorded 26 different karyotypes exhibited by 50 individuals from the hybrid population. Of the 26 karyotypes, only 14% presented a parental-like configuration, and none had the combinations of 2n and AN expected for an F1 hybrid. The remaining karyotypes were alternative hybrid forms, with 2n varying from 42 to 46 and AN from 68...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome polymorphism; Subterranean rodent; Karyotypes; Microsatellite.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600014

Estimating gain by use of a classic selection index under multicollinearity in wheat (Triticum aestivum)
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Samuel Pereira de; Cruz,Cosme Damião; Carvalho,Claudio Guilherme Portela de.
It was shown that the classic selection index, under multicollinearity, could not give simultaneous gains for wheat grain production and its primary components. This was due to the instability and, consequently, low precision of the coefficient index estimates. A modification of the prediction process of the index was proposed to avoid the adverse effects of multicollinearity, adopting a procedure based on ridge regression theory. The modified classic selection index, or ridge index, gave more statistically viable index coefficient estimates and gains for all of the characters evaluated. However, lower gains for number of grains per spike and grain yield were obtained, when compared to those obtained with selection for grain yield.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100021

Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers
Provedor de dados: Genetics and Molecular Biology
Autores: Kasuga,Ikuma; Paré,Peter D.; Sandford,Andrew J.
We established a specific genotyping assay for HLA-A*01, which is one of the most frequently found HLA-A alleles in the Caucasian population. This assay uses the polymerase chain reaction (PCR) with allele group-specific primers (ASP). HLA-A*01 group-specific primers were designed for exon 3 of the HLA-A gene, based on the recent HLA-sequence alignment. Both sense and anti-sense primers were designed with completely matched sequences to each specific HLA-A*01 allele, but mismatched by at least 1 nucleotide to all other known class I HLA alleles. By the use of these primers and stringent PCR conditions, we successfully genotyped the HLA-A*01 group alleles and achieved greater accuracy than previous methods.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA-A1; PCR; Allele group-specific primers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200003

The karyotype of Alouatta fusca clamitans from Rio de Janeiro, Brazil: Evidence for a y-autosome translocation
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Edivaldo H.C. de; Lima,Margarida M.C. de; Sbalqueiro,Ives José; Pissinati,Alcides.
The chromosome complements of four males of Alouatta fusca clamitans, caught in Rio de Janeiro State, Brazil, were analyzed by G-, C-, and NOR-banding techniques. The diploid number found was 49 in all the specimens. The presence of a heteromorphic pair of submetacentric chromosomes in the analyzed specimens, not present in males and females with 2n = 50 previously reported, and its G-banding pattern, led us to assume that this pair is involved in a Y-autosome translocation. Thus, the sex determination system appears modified to X1X1X2X2 /X1X2Y. Heterochromatic segments were found in the pericentromeric region of all the chromosomes, in the telomeric region of the short arm in pair 2, in the complete length of the short arm of pairs 5 and 6 and in the...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300012

Meiosis of anther culture regenerants in asparagus (Asparagus officinalis L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Galli,Leonardo; Viégas,Judith; Augustin,Eliane; Eckert,Marcia Ines; Silva,João Baptista da.
Pollen mother cells obtained from regenerated plants of asparagus (Asparagus officinalis L.), in a population composed exclusively of male plants, through the process of anther culture from the hybrid G27 X 22-8, were analyzed during meiosis. It was observed that, during theprocess of anther culture by organogenesis, the pollen mother cells of the regenerants had great genomic instability, as evidenced by disturbances in all the meiotic phases of the first and second division. Furthermore, structural chromosomal abnormalities, in addition to aneuploidy and polyploidy, were observed.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100016

High genetic diversity and connectivity in Colossoma macropomum in the Amazon basin revealed by microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Fazzi-Gomes,Paola; Guerreiro,Sávio; Palheta,Glauber David Almeida; Melo,Nuno Filipe Alves Correa de; Santos,Sidney; Hamoy,Igor.
Abstract Colossoma macropomum is the second largest scaled fish of the Amazon. It is economically important for commercial fisheries and for aquaculture, but few studies have examined the diversity and genetic structure of natural populations of this species. The aim of this study was to investigate the levels of genetic variability and connectivity that exist between three natural populations of C. macropomum from the Amazon basin. In total, 247 samples were collected from the municipalities of Tefé, Manaus, and Santarém. The populations were genotyped using a panel of 12 multiplex microsatellite markers. The genetic diversity found in these populations was high and similar to other populations described in the literature. These populations showed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tambaqui; Genetic variability; Gene flow; Genetic structure; Single sequence repeats.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100142

Microsatellites in Portuguese autochthonous horse breeds: usefulness for parentage testing
Provedor de dados: Genetics and Molecular Biology
Autores: Luís,C.; Cothran,E.Gus; Oom,M.M.
The use of DNA technology for parentage testing is increasing every day. Most laboratories have improved their exclusion probabilities (PE) by the addition of DNA microsatellite loci to standard blood-typing results. The efficacy of each locus depends on the number of alleles detected and their frequencies in the breeds tested. Here we analyzed the usefulness of six microsatellites for routine parentage testing in three Portuguese autochthonous horse breeds: Lusitano, Sorraia and Garrano. The DNA loci analyzed - ASB2, HMS3, HMS7, HTG4, HTG10, and VHL20 - were chosen based upon the polymorphism detected in other breeds with Iberian horse influence. The estimated probability of exclusion of wrongly named parents (PE) was high, with values ranging from 88.5%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; DNA; Portuguese horse breeds; Parentage testing.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200003

Estimates of repeatability and heritability of productive and reproductive traits in a herd of Jersey cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Roman,R.M.; Wilcox,C.J.; Martin,F.G.
Estimates of the repeatability and heritability of 19 measures of performance in Jersey cows were obtained using an animal model with a relationship matrix and a derivative-free restricted maximum likelihood algorithm. The data consisted of 935 records for 374 cows by 69 sires over the period 1969-1987. The estimates were similar to those obtained by ordinary least squares methods reported for the same data set and in other studies, but had smaller error variances. A likelihood ratio test showed agreement between these heritability estimates and those in the literature. The heritability estimates of milk, fat, protein, lactose-mineral, solids-not-fat, and total solids yields were about 0.25; for the corresponding percentages, and for the protein to fat and...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100021

Myeloproliferative syndrome of monosomy 7: a brief report
Provedor de dados: Genetics and Molecular Biology
Autores: Marques-Salles,Terezinha de Jesus; Soares-Ventura,Eliane Maria; Oliveira,Nathalia Lopes de; Silva,Mariluze; Assis,Reijane; Morais,Vera Lúcia Lins de; Otero,Luize; Fernandez,Teresa; Pombo-de-Oliveira,Maria do Socorro; Muniz,Maria Tereza Cartaxo; Santos,Neide.
We report the case of a five-month-old black male infant who had recurrent episodes of respiratory infections and also presented anemia and enlargements of the spleen, liver and lymphnodes. Hematological analysis revealed morphological abnormalities with megaloblastic dyserythropoiesis, while fetal hemoglobin assaying showed normal levels. Conventional and molecular cytogenetic analysis revealed monosomy of chromosome 7. Despite all therapeutic efforts during allogenic bone marrow transplantation, the child died due to generalized infection. The clinical and genetic distinctions between monosomy 7 syndrome and myelodysplastic disorders in childhood are discussed.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leukemia; Monosomy 7; Myeloproliferative syndrome.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100007

Phylogenetic relationships within Chamaecrista sect. Xerocalyx (Leguminosae, Caesalpinioideae) inferred from the cpDNA trnE-trnT intergenic spacer and nrDNA ITS sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Torres,Davi Coe; Lima,João Paulo Matos Santos; Fernandes,Afrânio Gomes; Nunes,Edson Paula; Grangeiro,Thalles Barbosa.
Chamaecrista belongs to subtribe Cassiinae (Caesalpinioideae), and it comprises over 330 species, divided into six sections. The section Xerocalyx has been subjected to a profound taxonomic shuffling over the years. Therefore, we conducted a phylogenetic analysis using a cpDNA trnE-trnT intergenic spacer and nrDNA ITS/5.8S sequences from Cassiinae taxa, in an attempt to elucidate the relationships within this section from Chamaecrista. The tree topology was congruent between the two data sets studied in which the monophyly of the genus Chamaecrista was strongly supported. Our analyses reinforce that new sectional boundaries must be defined in the Chamaecrista genus, especially the inclusion of sections Caliciopsis and Xerocalyx in sect. Chamaecrista,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogeny; ITS/5.8S; Chamaecrista; Xerocalyx; TrnE-trnT intergenic spacer.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200014

A proposal of a standardised nomenclature for terminal minute sister chromatid exchanges
Provedor de dados: Genetics and Molecular Biology
Autores: Drets,Máximo E.; Santiñaque,Federico F.; Obe,Günter.
We described spontaneous minute sister chromatid exchanges (SCE) in telomeric regions of human and Chinese hamster ovary (CHO) chromosomes more than 10 years ago. These structures, which we called t-SCE, were detected by means of highly precise quantitative microphotometrical scanning and computer graphic image analysis. Recently, several authors using the CO-FISH method also found small SCEs in telomeric regions and called them T-SCE. The use of different terms for designating the same phenomenon should be avoided. We propose ter SCE as a uniform nomenclature for minute telomeric SCEs.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Minute telomeric SCEs; Chromosome nomenclature.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300007

Genetic diversity analysis of Cuban traditional rice (Oryza sativa L.) varieties based on microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Alvarez,Alba; Fuentes,Jorge Luis; Puldón,Violeta; Gómez,Pedro Julio; Mora,Leonor; Duque,Miriam C.; Gallego,Gerardo; Tohme,Joe M.
Microsatellite polymorphism was studied in a sample of 39 traditional rice (Oryza sativa L.) varieties and 11 improved varieties widely planted in Cuba. The study was aimed at assessing the extent of genetic variation in traditional and improved varieties and to establish their genetic relationship for breeding purposes. Heterozygosity was analyzed at each microsatellite loci and for each genotype using 10 microsatellite primer pairs. Between varieties genetic relationship was estimated. The number of alleles per microsatellite loci was 4 to 8, averaging 6.6 alleles per locus. Higher heterozygosity (H) was found in traditional varieties (H TV = 0.72) than in improved varieties (H IV = 0.42), and 68% of the total microsatellite alleles were found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Microsatellite; Rice; Traditional germplasm.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600014

Does the chromosomal position of 35S rDNA sites influence their transcription? A survey on Nothoscordum species (Amaryllidaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Báez,Mariana; Souza,Gustavo; Guerra,Marcelo.
Abstract 35S ribosomal DNA (rDNA) sites are the regions where the ribosomal genes 18S, 5.8S and 25S, responsible for the formation of the nucleoli, are found. The fact that rDNA sites have non-random distribution on chromosomes suggests that their positions may influence their transcription. To identify if the preferentially transcribed rDNA sites occupy specific position, six species (nine cytotypes) of the genus Nothoscordum were analyzed using two different techniques to impregnate the nucleolar organizer regions (NORs) with silver nitrate. Both techniques strongly stained NORs, but one of them also stained the proximal region of all chromosomes, suggesting the existence of another group of argentophilic proteins in this region. In species with rDNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nucleolus; Ribosomal genes; Preferential activation of NORs; Nothoscordum.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100305

Phylogeographic analyses of the pampas cat (Leopardus colocola; Carnivora, Felidae) reveal a complex demographic history
Provedor de dados: Genetics and Molecular Biology
Autores: da Silva Santos,Anelisie; Trigo,Tatiane Campos; de Oliveira,Tadeu Gomes; Silveira,Leandro; Eizirik,Eduardo.
Abstract The pampas cat is a small felid that occurs in open habitats throughout much of South America. Previous studies have revealed intriguing patterns of morphological differentiation and genetic structure among its populations, as well as molecular evidence for hybridization with the closely related L. tigrinus. Here we report phylogeographic analyses encompassing most of its distribution (focusing particularly on Brazilian specimens, which had been poorly sampled in previous studies), using a novel dataset comprising 2,143 bp of the mitogenome, along with previously reported mtDNA sequences. Our data revealed strong population strutucture and supported a west-to-east colonization process in this species’ history. We detected two population expansion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogeography; Population genetics; Mitochondrial DNA; Conservation genetics; Historical demography.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200273

small ORFs: A new class of essential genes for development
Provedor de dados: Genetics and Molecular Biology
Autores: Albuquerque,João Paulo; Tobias-Santos,Vitória; Rodrigues,Aline Cáceres; Mury,Flávia Borges; Fonseca,Rodrigo Nunes da.
Genes that contain small open reading frames (smORFs) constitute a new group of eukaryotic genes and are expected to represent 5% of the Drosophila melanogaster transcribed genes. In this review we provide a historical perspective of their recent discovery, describe their general mechanism and discuss the importance of smORFs for future genomic and transcriptomic studies. Finally, we discuss the biological role of the most studied smORF so far, the Mlpt/Pri/Tal gene in arthropods. The pleiotropic action of Mlpt/Pri/Tal in D. melanogaster suggests a complex evolutionary scenario that can be used to understand the origins, evolution and integration of smORFs into complex gene regulatory networks.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tribolium; Mlpt; Pri; Tarsal-less; Drosophila.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300278

Comparative transcriptome profile of the leaf elongation zone of wild barley (Hordeum spontaneum) eibi1 mutant and its isogenic wild type
Provedor de dados: Genetics and Molecular Biology
Autores: Zhou,Qin; Wang,Aidong; Duan,Ruijun; Yan,Jun; Zhao,Gang; Nevo,Eviatar; Chen,Guoxiong.
Abstract The naturally occurring wild barley mutant eibi1/hvabcg31 suffers from severe water loss due to the permeable leaf cuticle. Eibi1/HvABCG31 encodes a full ATP-binding cassette (ABC) transporter, HvABCG31, playing a role in cutin deposition in the elongation zone of growing barley leaves. The eibi1 allele has pleiotropic effects on the appearance of leaves, plant stature, fertility, spike and grain size, and rate of germination. Comparative transcriptome profile of the leaf elongation zone of the eibi1 mutant as well as its isogenic wild type showed that various pathogenesis-related genes were up-regulated in the eibi1 mutant. The known cuticle-related genes that we analyzed did not show significant expression difference between the mutant and wild...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ABC transporter; Defense genes; Desiccation tolerance; Plant cuticle; RNA-Seq.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500834

ELAG: 10 successful years
Provedor de dados: Genetics and Molecular Biology
Autores: Giuglianiand,Roberto; Matte,Ursula.
Tipo: Info:eu-repo/semantics/other
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200001

Hermaphroditism in the rubber tree Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell. Arg. - II
Provedor de dados: Genetics and Molecular Biology
Autores: Cuco,Silvia Marina; Bandel,Gerhard.
Flowers of three Hevea brasiliensis clones, RRIM 527, RRIM 600 and GT 1, were analyzed under stereomicroscope and scanning electron microscope, aiming to observe hermaphroditism rates. Results showed 71.49% hermaphrodite flowers, 29.83% of which exhibited incompletely developed, residual anthers. The scanning electron microscope analysis did not detect differences in anther epidermis of male and bisexual flowers of RRIM 600 and RRIM 527. In GT 1 clone (sterile male), the anther epidermis was already weak at the beginning of floral development and completely wrinkled at the end of maturation. Consequently, the anthers were empty by this stage.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400019

Chromosome comparison among five species of Neotropical cichlids of Cichlasoma and Gymnogeophagus (Perciformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Pires,Larissa Bettin; Usso,Mariana Campaner; Giuliano-Caetano,Lucia; Dias,Ana Lúcia.
Abstract The genera Cichlasoma and Gymnogeophagus belong to the subfamily Cichlinae, the only one in Neotropical cichlids. Cichlasoma dimerus, C. paranaense, C. portalegrense, Gymnogeophagus rhabdotus, and G. lacustris were collected at different points in the Paranapanema and Paraguay basins and the Lagoon of Patos hydrographic system. In addition to conventional analysis, CMA3 fluorochrome staining, and FISH with 18S rDNA probe were performed. All species had a diploid number equal to 48, with interand intraspecific differences in karyotype formulae. All species presented a single AgNOR site, except G. rhabdotus and the C. paranaense population of the Paranapanema River, which revealed more than one pair of nucleolar chromosomes. AgNORs were coincident...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome banding; Fish cytogenetics; Karyotype diversification; Ribosomal DNA.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400203

Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms
Provedor de dados: Genetics and Molecular Biology
Autores: Alvarez-Moya,Carlos; Reynoso Silva,Mónica; Valdez Ramírez,Carlos; Gómez Gallardo,David; León Sánchez,Rafael; Canales Aguirre,Alejandro; Feria Velasco,Alfredo.
There is considerable controversy with regard to the genotoxicity of glyphosate, with some reports stating that this compound is non-toxic for fish, birds and mammals. In this work, we used the comet assay to examine the genotoxicity of glyphosate isopropylamine (0.7, 7, 70 and 700 µM) in human lymphocytes, erythrocytes of Oreochromis niloticus and staminal nuclei of Tradescantia (4430) in vitro and in vivo. Cells, nuclei and fish that had and had not been exposed to 5 mM N-nitrosodiethylamine (NDEA) were used as positive and negative controls, respectively. Significant (p &lt; 0.01) genetic damage was observed in vivo and in vitro in all cell types and organisms tested. Human lymphocytes and Tradescantia hairs showed lower genetic damage in vivo compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; DNA damage; Genotoxicity; Glyphosate.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100016

Using the FORESTS and KEGG databases to investigate the metabolic network of Eucalyptus
Provedor de dados: Genetics and Molecular Biology
Autores: Mombach,José C.M.; Lemke,Ney; Silva,Norma M. da; Ferreira,Rejane A.; Isaia Filho,Eduardo; Barcellos,Cláudia K.; Ormazabal,Rodrigo J.
In this work we apply a bioinformatics approach to determine the most important enzymes of the metabolic network of Eucalyptus to determine the coverage of the genome in the FORESTS library. We conclude that the library does not cover completely the metabolism of the organism. However, some important pathways could be analyzed, especially the lignin synthesis. We found that four of the most important enzymes predicted are involved in this pathway.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Eucalyptus; Metabolism.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400018

Cytological analysis of hybrids among triticales and trigopiros
Provedor de dados: Genetics and Molecular Biology
Autores: Fradkin,Maia; Greizerstein,Eduardo; Paccapelo,Héctor; Ferreira,Víctor; Grassi,Ezequiel; Poggio,Lidia; Ferrari,María Rosa.
We studied three different tricepiros: (Don Santiago x Don Noé), (Cumé x Horovitz) and (Cumé x Don Noé). The tricepiro (Don Santiago x Don Noé) was obtained by crossing the triticale Don Santiago INTA (AABBRR, 2n = 6x = 42) with the trigopiro Don Noé INTA (AABBDDJJ, 2n = 8x = 56). The number of chromosomes for the F1 was 2n = 49, the most frequent meiotic configuration being 14 bivalents and 21 univalents. The univalents were situated in the periphery of the equatorial plane, whereas the bivalents were located in the central zone. The chromatids in some of the univalents split when bivalents underwent reductional division in anaphase I. There were few laggard chromosomes or chromatids at this phase. The number of chromosomes (2n = 48-58) was high and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hybrids; Meiosis; Tricepiro; Univalents; Reductional division.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400022

Genetic diversity characterization of cassava cultivars (Manihot esculenta Crantz).: I) RAPD markers
Provedor de dados: Genetics and Molecular Biology
Autores: Colombo,Carlos; Second,Gérard; Valle,Tereza Losada; Charrier,André.
RAPD markers were used to investigate the genetic diversity of 31 Brazilian cassava clones. The results were compared with the genetic diversity revealed by botanical descriptors. Both sets of variates revealed identical relationships among the cultivars. Multivariate analysis of genetic similarities placed genotypes destinated for consumption "in nature" in one group, and cultivars useful for flour production in another. Brazil’s abundance of landraces presents a broad dispersion and is consequently an important resource of genetic variability. The botanical descriptors were not able to differentiate thirteen pairs of cultivars compared two-by-two, while only one was not differentiated by RAPD markers. These results showed the power of RAPD markers over...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100018

Indirect selection criteria against clean wool colour in Corriedale sheep and their effects on wool production traits
Provedor de dados: Genetics and Molecular Biology
Autores: Benavides,M.V.; Maher,A.P.
The potential of greasy wool colour subjective assessment Visual Colour Score (VCS) and the yellow predictive test (YPC) as indirect selection criteria for reduction of clean wool colour (CWC) in Corriedale sheep was examined. The heritability of these wool colour traits and the wool production traits, greasy (GFW) and clean fleece weights (CFW), and mean fibre diameter (MFD) and the phenotypic and genetic correlations among these traits were estimated from a Corriedale flock using AIREML procedures. A high genetic correlation between YPC and CWC was observed, indicating that YPC could be a suitable indirect selection criterium for CWC. However, direct selection against CWC was predicted to produce faster genetic improvements in CWC than that expected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wool yellowing susceptibility; Genetic parameter estimates; Selection responses.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200005

Mitochondrial DNA mapping of social-biological interactions in Brazilian Amazonian African-descendant populations
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Bruno Maia; Bortolini,Maria Cátira; Santos,Sidney Emanuel Batista dos; Ribeiro-dos-Santos,Ândrea Kely Campos.
The formation of the Brazilian Amazonian population has historically involved three main ethnic groups, Amerindian, African and European. This has resulted in genetic investigations having been carried out using classical polymorphisms and molecular markers. To better understand the genetic variability and the micro-evolutionary processes acting in human groups in the Brazilian Amazon region we used mitochondrial DNA to investigate 159 maternally unrelated individuals from five Amazonian African-descendant communities. The mitochondrial lineage distribution indicated a contribution of 50.2% from Africans (L0, L1, L2, and L3), 46.6% from Amerindians (haplogroups A, B, C and D) and a small European contribution of 1.3%. These results indicated high genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: African mtDNA; Amazon population; Amerindian mtDNA; HVS-I.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100002

New microdeletion and microduplication syndromes: a comprehensive review
Provedor de dados: Genetics and Molecular Biology
Autores: Nevado,Julián; Mergener,Rafaella; Palomares-Bralo,María; Souza,Karen Regina; Vallespín,Elena; Mena,Rocío; Martínez-Glez,Víctor; Mori,María Ángeles; Santos,Fernando; García-Miñaur,Sixto; García-Santiago,Fé; Mansilla,Elena; Fernández,Luis; Torres,María Luisa de; Riegel,Mariluce; Lapunzina,Pablo.
Several new microdeletion and microduplication syndromes are emerging as disorders that have been proven to cause multisystem pathologies frequently associated with intellectual disability (ID), multiple congenital anomalies (MCA), autistic spectrum disorders (ASD) and other phenotypic findings. In this paper, we review the "new" and emergent microdeletion and microduplication syndromes that have been described and recognized in recent years with the aim of summarizing their main characteristics and chromosomal regions involved. We decided to group them by genomic region and within these groupings have classified them into those that include ID, MCA, ASD or other findings. This review does not intend to be exhaustive but is rather a quick guide to help...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microdeletion; Microduplication; Chromosome rearrangement; Novel deletions; Novel duplications.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200007

Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Ribeiro,Leila Braga; Matoso,Daniele Aparecida; Feldberg,Eliana.
The Serrasalmidae family is composed of a number of commercially interesting species, mainly in the Amazon region where most of these fishes occur. In the present study, we investigated the genomic organization of the 18S and 5S rDNA and telomeric sequences in mitotic chromosomes of four species from the basal clade of the Serrasalmidae family: Colossoma macropomum, Mylossoma aureum, M. duriventre, and Piaractus mesopotamicus, in order to understand the chromosomal evolution in the family. All the species studied had diploid numbers 2n = 54 and exclusively biarmed chromosomes, but variations of the karyotypic formulas were observed. C-banding resulted in similar patterns among the analyzed species, with heterochromatic blocks mainly present in centromeric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5S rDNA; Telomeric sequences; Tambaqui and pacu.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100009

Regulation of MMP2 and MMP9 metalloproteinases by FSH and growth factors in bovine granulosa cells
Provedor de dados: Genetics and Molecular Biology
Autores: Portela,Valerio M.; Veiga,Angela; Price,Christopher A.
Matrix metalloproteinases (MMP) are key enzymes involved in tissue remodeling. Within the ovary, they are believed to play a major role in ovulation, and have been linked to follicle atresia. To gain insight into the regulation of MMPs, we measured the effect of hormones and growth factors on MMP2 and MMP9 mRNA levels in non-luteinizing granulosa cells in serum-free culture. FSH and IGF1 both stimulated estradiol secretion and inhibited MMP2 and MMP9 mRNA abundance. In contrast, EGF and FGF2 both inhibited estradiol secretion but had no effect on MMP expression. At physiological doses, none of these hormones altered the proportion of dead cells. Although we cannot link MMP expression with apoptosis, the specific down regulation by the gonadotropic hormones...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metalloproteinase; Gonadotropin; Growth factor; Granulosa.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300014

Increased micronucleated cell frequency related to exposure to radiation emitted by computer cathode ray tube video display monitors
Provedor de dados: Genetics and Molecular Biology
Autores: Carbonari,Karina; Gonçalves,Luciane; Roth,Daniela; Moreira,Patrick; Fernández,Ricardo; Martino-Roth,Maria da Graça.
It is well recognized that electromagnetic fields can affect the biological functions of living organisms at both cellular and molecular level. The potential damaging effects of electromagnetic fields and very low frequency and extremely low frequency radiation emitted by computer cathode ray tube video display monitors (VDMs) has become a concern within the scientific community. We studied the effects of occupational exposure to VDMs in 10 males and 10 females occupationally exposed to VDMs and 20 unexposed control subjects matched for age and sex. Genetic damage was assessed by examining the frequency of micronuclei in exfoliated buccal cells and the frequency of other nuclear abnormalities such as binucleated and broken egg cells. Although there were no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronucleus test; Mutagenicity; Electromagnetic fields.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300024

Analysis of energetically biased transcripts of viruses and transposable elements
Provedor de dados: Genetics and Molecular Biology
Autores: Secolin,Rodrigo; Pascoal,Vinícius D'Ávila Bitencourt; Lopes-Cendes,Iscia; Pereira,Tiago Campos.
RNA interference (RNAi) is a natural endogenous process by which double-stranded RNA molecules trigger potent and specific gene silencing in eukaryotic cells and is characterized by target RNA cleavage. In mammals, small interfering RNAs (siRNAs) are the trigger molecules of choice and constitute a new class of RNA-based antiviral agents. In an efficient RNAi response, the antisense strand of siRNAs must enter the RNA-induced silencing complex (RISC) in a process mediated by thermodynamic features. In this report, we hypothesize that silent mutations capable of inverting thermodynamic properties can promote resistance to siRNAs. Extensive computational analyses were used to assess whether continuous selective pressure that promotes such mutations could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNAi; SiRNA; Synonymous mutation; Viral evolution.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500022

Population analysis of the GLB1 gene in South Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Baiotto,Cléia; Sperb,Fernanda; Matte,Ursula; Silva,Cláudia Dornelles da; Sano,Renata; Coelho,Janice Carneiro; Giugliani,Roberto.
Infantile GM1 gangliosidosis is caused by the absence or reduction of lysosomal beta-galactosidase activity. Studies conducted in Brazil have indicated that it is one of the most frequent lysosomal storage disorders in the southern part of the country. To assess the incidence of this disorder, 390 blood donors were tested for the presence of two common mutations (1622-1627insG and R59H) in the GLB1 gene. Another group, consisting of 26 GM1 patients, and the blood donors were tested for the presence of two polymorphisms (R521C and S532G), in an attempt to elucidate whether there is a founder effect. The frequencies of the R59H and 1622-1627insG mutations among the GM1 patients studied were 19.2% and 38.5%, respectively. The frequency of polymorphism S532G...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GM1 gangliosidosis; Beta-galactosidase; GLB1 gene; Founder effect; Linkage disequilibrium.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100009

Germline DNA copy number variation in individuals with Argyrophilic grain disease reveals CTNS as a plausible candidate gene
Provedor de dados: Genetics and Molecular Biology
Autores: Villela,Darine; Kimura,Lilian; Schlesinger,David; Gonçalves,Amanda; Pearson,Peter L.; Suemoto,Claudia K.; Pasqualucci,Carlos; Krepischi,Ana Cristina; Grinbergand,Lea T.; Rosenberg,Carla.
Argyrophilic grain disease (AGD) is a progressive neurodegenerative disease of the human brain that has never been associated to a particular gene locus. In the present study, we report the results of a CNV investigation in 29 individuals whose anatomopathologic investigation of the brain showed AGD. Rare CNVs were identified in six patients (21%), in particular a 40 kb deletion at 17p13.2 encompassing the CTNS gene. Homozygote mutations in CTNS are known to cause cystinosis, a disorder characterized by the intralysosomal accumulation of cystine in all tissues. We present the first CNV results in individuals presenting AGD and a possible candidate gene implicated in the disorder.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Argyrophilic grain disease; Copy number variations; CNVs; Array-CGH; CTNS.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400006

Cytogenetic analysis in western Atlantic snappers (Perciformes, Lutjanidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Rocha,Érika Cruz; Molina,Wagner Franco.
The Lutjanidae or snappers are a family of perciform fishes, mainly marine but with some members living in estuaries and entering fresh water to feed. Some are important food fish. Cytogenetic data for Lutjanidae are scarce. In the present work, we cytogenetically characterized through conventional Giemsa staining techniques, Ag-NOR and C-banding the species Ocyurus chrysurus, Lutjanus analis, L. alexandrei, L. cyanopterus, L. jocu and L. synagris, all found along the Brazilian coast. Karyotype analysis of all six species showed a modal value of 2n = 48 acrocentric chromosomes. Single NORs were found at pericentromeric position on the long arms of the 2nd pair in O. chrysurus, L. alexandrei and L. cyanopterus, on the 5th pair in L. analis and on the 23rd...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome evolution; Fish cytogenetics; Lutjanidae; Lutjanus; Ocyurus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300011

Genetic relationships among native americans based on beta-globin gene cluster haplotype frequencies
Provedor de dados: Genetics and Molecular Biology
Autores: Mousinho-Ribeiro,Rita de Cassia; Pante-de-Sousa,Gabriella; Santos,Eduardo José Melo dos; Guerreiro,João Farias.
The distribution of b-globin gene haplotypes was studied in 209 Amerindians from eight tribes of the Brazilian Amazon: Asurini from Xingú, Awá-Guajá, Parakanã, Urubú-Kaapór, Zoé, Kayapó (Xikrin from the Bacajá village), Katuena, and Tiriyó. Nine different haplotypes were found, two of which (n. 11 and 13) had not been previously identified in Brazilian indigenous populations. Haplotype 2 (+ - - - -) was the most common in all groups studied, with frequencies varying from 70% to 100%, followed by haplotype 6 (- + + - +), with frequencies between 7% and 18%. The frequency distribution of the b-globin gene haplotypes in the eighteen Brazilian Amerindian populations studied to date is characterized by a reduced number of haplotypes (average of 3.5) and low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA polymorphisms; Brazilian Amerindians; Genetic diversity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300002

Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis
Provedor de dados: Genetics and Molecular Biology
Autores: Bravo,Juliana Pereira; Hoshino,Andrea Akemi; Angelici,Carla Maria Lara C.D.; Lopes,Catalina Romero; Gimenes,Marcos Aparecido.
The Arachis section is the most important of the nine sections of the genus Arachis because it includes the cultivated peanut, Arachis hypogaea. The genetic improvement of A. hypogaea using wild relatives is at an early stage of development in spite of their potential as sources of genes, including those for disease and pests resistance, that are not found in the A. hypogaea primary gene pool. Section Arachis species germplasm has been collected and maintained in gene banks and its use and effective conservation depends on our knowledge of the genetic variability contained in this material. Microsatellites are routinely used for the analysis of genetic variability because they are highly polymorphic and codominant. The objective of this study was to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Germplasm; Peanut; Transferability; Wild species.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300021

Methylenetetrahydrofolate reductase polymorphisms in myeloid leukemia patients from Northeastern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Barbosa,Cynara Gomes; Souza,Claudio Lima; Moura Neto,José Pereira de; Arruda,Maria da Glória Bomfim; Barreto,José Henrique; Reis,Mitermayer Galvão; Goncalves,Marilda Souza.
Methylenetetrahydrofolate reductase (MTHFR: EC 1.5.1.20) polymorphisms are associated to acute lymphoid leukemia in different populations. We used the polymerase chain reaction and the restriction fragment length polymorphism method (PCR-RFLP) to investigate MTHFR C677T and A1298C polymorphism frequencies in 67 patients with chronic myeloid leukemia (CML), 27 with acute myeloid leukemia FAB subtype M3 (AML-M3) and 100 apparently healthy controls. The MTHFR mutant allele frequencies were as follows: CML = 17.2% for C677T, 21.6% for A1298C; AML-M3 = 22.2% for C677T, 24.1% for A1298C; and controls = 20.5% for C677T, 21% for A1298C. Taken together, our results provide evidence that MTHFR polymorphisms have no influence on the development of CML or AML-M3.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: AML; CML; MTHFR polymorphisms.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100005

Genetic trends in the expected progeny difference of the asymptotic weight of Nelore females
Provedor de dados: Genetics and Molecular Biology
Autores: Garnero,Analía del Valle; Marcondes,Cintia Righetti; Gunski,Ricardo José; Oliveira,Henrique Nunes de; Lôbo,Raysildo Barbosa.
There are few studies on weight covering the full life cycle of Zebu cattle, and there is no entire growth description or mean growth pattern for animals belonging to this breed. In order to provide such data, 1,158 Nelore females born between 1985 and 1995 were weighed 14,563 times from birth to full growth maturity, in ten herds spread over seven Brazilian states. The Von Bertalanffy, Brody, logistic and Gompertz non-linear models were used to obtain the asymptotic weights (A) and the maturation rates (K). The (co)variance and breeding value components for A and K were obtained by using the multiple trait derivative free restricted maximum likelihood method under the animal model. Genetic trends were calculated in function of the mean expected progeny...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adult weight; Bovine(s); Genetic trends; Maturation rate; Simulation with growth curves.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400012

Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant
Provedor de dados: Genetics and Molecular Biology
Autores: Rêgo,Elizanilda R. do; Finger,Fernando L.; Casali,Vicente W.D.; Cardoso,Antônio A.
A naturally occurring yellow tomato fruit mutant cv. Santa Clara was reciprocally crossed with the red wild type, after which F1 plants were self pollinated or backcrossed with both parents. Plants from F1 generations produced all fruits with a homogeneous deep red color when ripe. F2 plants showed a 3:1 red:yellow segregation of fruit color, and 100% red when backcrossed with red wild type or 1:1 red:yellow segregation in backcrosses with the yellow mutant; hence, yellow fruit color was determined by a recessive allele. Based on reciprocal crosses, fruit color is unlikely to be determined by maternal genes. Accumulation of lycopene dropped by 99.3% and&lt;FONT FACE="Symbol"&gt; b&lt;/font&gt;-carotene by 77% in ripe yellow fruits, compared to the red wild...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100019

A novel c.197T ® A variant among Brazilian neonates with glucose-6-phosphate dehydrogenase deficiency
Provedor de dados: Genetics and Molecular Biology
Autores: Moura Neto,José Pereira de; Dourado,Marcos Vinícius; Reis,Mitermayer Galvão dos; Gonçalves,Marilda Souza.
Glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49) deficiency is the most common enzyme deficiency worldwide, causing a spectrum of diseases including neonatal hyperbilirubinemia and acute or chronic hemolysis. We used the methemoglobin reduction test and G6PD electrophoresis to screen 655 neonates (354 females and 301 males) for common G6PD mutations in the city of Salvador in the Northeastern Brazilian state Bahia and found that 66 (10.1%) were G6PD-deficient (41 females and 25 males). The 66 (10.1%) G6PD-deficient neonates were assessed for the c.376 A -&gt; G (exon 5) and c.202 G -&gt; A (exon 4) mutations using the polymerase chain reaction and restriction enzyme fragment length polymorphism (PCR-RFLP) analysis and the results validated by DNA...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glucose-6-phosphate dehydrogenase deficiency; G6PD mutations; Neonatal screening.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100006

Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays
Provedor de dados: Genetics and Molecular Biology
Autores: Bellini,Marilanda Ferreira; Cabrioti,Leonardo Neves; Terezan,Ana Paula; Jordão,Berenice Quinzani; Ribeiro,Lúcia Regina; Mantovani,Mário Sérgio.
Functional food investigations have demonstrated the presence of substances that could be beneficial to human health when consumed. However, the toxic effects of some substances contained in foods have been determined. Reported medicinal and nutritive properties have led to the extensive commercialization of the basidiomycete fungi Agaricus blazei Murrill (sensu Heinemann), also known as Agaricus brasiliensis Wasser et al., Agaricus subrufescens Peck or the Brazilian medical mushroom (BMM). Different methanolic extract fractions (ME) of this mushroom were submitted to the cytokinesis-block micronucleus (CBMN) clastogenic assay and the hypoxanthine-guanine phosphoribosyl transferase locus (HGPRT) assay for gene mutation, both using Chinese hamster ovary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus blazei; Hgprt locus; Micronucleus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100021

Characterization of partial Hox gene sequences in annual fish of the subfamily Cynolebiatinae (Cyprinodontiformes, Rivulidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Gutiérrez,Verónica; Arezo,María José; García,Graciela.
Hox genes encode a family of transcription factors implicated in conferring regional identity along the anteroposterior axis in developing animal embryos. These genes are organized in genomic clusters, expressed collinearly and highly conserved in vertebrates. Among teleost, South American annual killifishes of the Cynolebiatinae subfamily represent an excellent model in development studies because their embryos are capable of undergoing reversible developmental arrest (diapause) at three well-defined morphological stages. They are also an excellent model for evolutionary studies due to the high rates of mutation of their mitochondrial genome, their karyotypic divergence and their morphological variability. In this study, three partial homeobox sequences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Subfamily Cynolebiatinae; Annual killifishes; Hox genes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300031

Comparative analysis of morphological and behavioral characters in the domestic dog and their importance in the reconstruction of phylogenetic relationships in canids
Provedor de dados: Genetics and Molecular Biology
Autores: Jordana,J.; Manteca,X.; Ribo,O.
Relationships among 25 dog breeds, classified a priori by their respective ancestral trunks, were studied using data from 29 morphological and 13 behavioral characteristics. Although a certain correlation was found between both types of traits (r = 0.13; P &amp;lt; 0.05), this relationship was not manifested, regarding the level of racial classification, in the obtained dendrograms. The relationships between breeds obtained from morphological data were more congruent than those obtained from behavioral data when compared with phylogenies from other sources of information (mainly electrophoretic analysis). This indicates that the morphological characters could give more and better complementary information than the behavioral ones in the reconstruction of...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100011

A model for the RecA protein of Mycoplasma synoviae
Provedor de dados: Genetics and Molecular Biology
Autores: Fonseca,Marbella Maria; Alarcon,Frank J.B.; Vasconcelos,Ana Tereza de; Agnez-Lima,Lucymara Fassarela.
In this work, we predict a structural model for the RecA protein from M. synoviae (MsRecA) by theoretical homology modeling and evaluate the occurrence of polymorphisms in this protein within several isolates of this species. The structural model suggested for MsRecA conserves the main domains present in MtRecA and EcRecA. The L1 and L2 regions showed six and three amino acid substitutions, respectively, which apparently do not affect the conformation and function of MsRecA. The C-terminal domain is shorter than that found in EcRecA and MtRecA, which may increase its capacity to bind dsDNA and displace SSB, compensating the absence of recombination initiation enzymes. The MS59 isolate RecA sequence showed one polymorphism which does not affect its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA repair; Recombination; RecA; Mycoplasma synoviae.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200018

Towards an optimal sampling strategy for assessing genetic variation within and among white clover (Trifolium repens L.) cultivars using AFLP
Provedor de dados: Genetics and Molecular Biology
Autores: Khanlou,Khosro Mehdi; Vandepitte,Katrien; Asl,Leila Kheibarshekan; Bockstaele,Erik Van.
Cost reduction in plant breeding and conservation programs depends largely on correctly defining the minimal sample size required for the trustworthy assessment of intra- and inter-cultivar genetic variation. White clover, an important pasture legume, was chosen for studying this aspect. In clonal plants, such as the aforementioned, an appropriate sampling scheme eliminates the redundant analysis of identical genotypes. The aim was to define an optimal sampling strategy, i.e., the minimum sample size and appropriate sampling scheme for white clover cultivars, by using AFLP data (283 loci) from three popular types. A grid-based sampling scheme, with an interplant distance of at least 40 cm, was sufficient to avoid any excess in replicates. Simulations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sampling strategy; White clover; AFLP; Genetic diversity parameters.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200015

Identification and characterization of homeobox genes in Eucalyptus
Provedor de dados: Genetics and Molecular Biology
Autores: Rocha,Graça Celeste Gomes; Corrêa,Régis Lopes; Borges,Anna Cristina Neves; Sá,Claudio Bustamante Pereira de; Alves-Ferreira,Márcio.
Homeobox genes encode transcriptional factors, usually involved in molecular control of plant developmental patterns. They can be divided into several classes according to conserved sequences within the homeobox region and the presence of specific additional sequences. Based on these conserved sequences, we developed a search procedure to identify possible homeobox genes in the Eucalyptus Genome Sequencing Project Consortium (FORESTs) database. We were able to identify 50 Eucalyptus sequences (EST-contigs) containing the homeodomain sequence. Phylogenetic analysis was applied to this ESTs-contigs and 44 of them were found to have similarities with one of three well-known homeobox classes: Bell, Knox and HD-Zip, and their sub-classes. However, no EST-contig...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant development; Abiotic stress; Eucalyptus; FORESTs; Phylogenetic analysis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400005

Functional classification of esterases from leaves of Aspidosperma polyneuron M. Arg. (Apocynaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Vanda Marilza de; Marques,Rosimar Maria; Lapenta,Ana Sílvia; Machado,Maria de Fátima P.S.
Polyacrylamide gel electrophoresis system (PAGE) and inhibition tests for biochemical characterization of alpha- and beta-esterases were used to obtain a functional classification of esterases fromAspidosperma polyneuron. The characterization of alpha- and beta-esterases from young leaves of A. polyneuron by the PAGE system showed fourteen esterase isozymes. The differential staining pattern showed that Est-2 isozyme hydrolyzes beta-naphthyl acetate; Est-6, Est-7 and Est-8 isozymes hydrolyze alpha-naphthyl acetate, and Est-1, Est-3, Est-4, Est-5, Est-9, Est-10, Est-11, Est-12, Est-13, and Est-14 isozymes hydrolyze both alpha- and b-naphthyl acetate. Inhibition pattern of a- and beta-esterases showed that Folidol is a more potent inhibitor that Malathion,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esterase classification; Peroba-rosa; Ops; Inhibition test.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200013

Genetic characterisation of populations of the critically endangered Goliath grouper ( Epinephelus itajara,  Serranidae) from the Northern Brazilian coast through analyses of mtDNA
Provedor de dados: Genetics and Molecular Biology
Autores: Silva-Oliveira,Gláucia C.; Rêgo,Péricles S. do; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
The Goliath grouper ( Epinephelus itajara ) is one of the most endangered species of fish of the subfamily Epinephelinae. Slow to develop and mature, and dependent on mangrove habitats for breeding, the species also suffers intense harvesting, which has reduced drastically in numbers in many areas. To contribute to the understanding of the characteristics of  E. itajara  populations, we conducted a molecular genetics study of the species, focusing on populations from the Northern Brazilian coast. The mtDNA control region (D-loop) of 116 individuals from five localities (Bragança, Ajuruteua, Parnaíba, Fortaleza and Natal) was analysed, and a sequence of 499 base pairs identified. Analyses of the sequences indicated that genetic variability was generally...
Palavras-chave: Goliath grouper; Epinephelus itajara; Control region; MtDNA; Population genetics.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500029

Characterization of PRLR and PPARGC1A genes in buffalo (Bubalus bubalis)
Provedor de dados: Genetics and Molecular Biology
Autores: Javed,Ruheena; Gautam,Sanjeev K.; Vijh,Ramesh K.; Tantia,Madhu S.
More than 40 million households in India depend at least partially on livestock production. Buffaloes are one of the major milk producers in India. The prolactin receptor (PRLR) gene and peroxisome proliferators activated receptor-γ coactivator 1-alpha (PPARGC1A) gene are reportedly associated with milk protein and milk fat yields in Bos taurus. In this study, we sequenced the PRLR and PPARGC1A genes in the water buffalo Bubalus bubalis. The PRLR and PPARGC1A genes coded for 581 and 819 amino acids, respectively. The B. bubalis PRLR gene differed from the corresponding Bos taurus at 21 positions and four differences with an additional arginine at position 620 in the PPARGC1A gene were found in the amino acid sequence. All of the changes were confirmed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bubalus bubalis; Buffalo; PRLR gene; PPARGC1A gene.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400011

Genetic differentiation and geographical Relationship of Asian barley landraces using SSRs
Provedor de dados: Genetics and Molecular Biology
Autores: Naeem,Rehan; Dahleen,Lynn; Mirza,Bushra.
Genetic diversity in 403 morphologically distinct landraces of barley (Hordeum vulgare L. subsp. vulgare) originating from seven geographical zones of Asia was studied using simple sequence repeat (SSR) markers from regions of medium to high recombination in the barley genome. The seven polymorphic SSR markers representing each of the chromosomes chosen for the study revealed a high level of allelic diversity among the landraces. Genetic richness was highest in those from India, followed by Pakistan while it was lowest for Uzbekistan and Turkmenistan. Out of the 50 alleles detected, 15 were unique to a geographic region. Genetic diversity was highest for landraces from Pakistan (0.70 ± 0.06) and lowest for those from Uzbekistan (0.18 ± 0.17). Likewise,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cluster analysis; Genetic diversity; Geographical differentiation; Hordeum vulgare; Principal component analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200017

Phylogeny, biogeography and divergence times in Passiflora (Passifloraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Muschner,Valéria C.; Zamberlan,Priscilla M.; Bonatto,Sandro L.; Freitas,Loreta B.
As part of a long-term investigation on the evolution of Passiflora L., we investigated the divergence ages of the genus and diversification of its subgenera, relating them with biogeographical and/or historical events, and other characteristics of this taxon. The main aim of the present work was to evaluate the biogeographic distribution of this genus to better understand its evolutionary history. This is the first time that representatives from South American and Old World Passifloraceae genera have been studied as a group comprising a total of 106 widely distributed species, with representative samples of the four suggested subgenera. Seven DNA regions were studied, comprising 7,431 nucleotides from plastidial, mitochondrial and nuclear genomes....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biogeography; Molecular phylogenetics analysis; Passionflowers; Plant evolution; Taxonomic classification.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600019

Bayesian inference in a quantitative genetic study of growth traits in Nelore cattle (Bos indicus)
Provedor de dados: Genetics and Molecular Biology
Autores: Faria,Carina Ubirajara de; Magnabosco,Cláudio de Ulhôa; Reyes,Arcadio de los; Lôbo,Raysildo Barbosa; Bezerra,Luiz Antônio Framartino; Sainz,Roberto Daniel.
The objective of this study was to estimate (co)variance components and genetic parameters for weights (W) and scrotal circumferences (SC) at 365 and 450 days of age, of Nelore (Bos indicus) cattle, using Bayesian inference in single and multiple-trait animal models. The fitted linear models included, besides the animal and residual random effects, the contemporary group (herd-year-season-sex-management group) and age-of-dam as "fixed effects". The analyses were carried out using a Gibbs sampler implemented through the MTGSAM program. The heritabilities (in parentheses) obtained fitting single-trait models were W365 (0.49), W450 (0.52), SC365 (0.68) and SC450 (0.66). Estimates of means, modes and medians for genetic parameters obtained from marginal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bayesian inference; Growth traits; Genetic parameters.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400007

Induction of somatic embryogenesis in soybean: physicochemical factors influencing the development of somatic embryos
Provedor de dados: Genetics and Molecular Biology
Autores: Bonacin,Gisele Aparecida; Di Mauro,Antonio Orlando; Oliveira,Roberto Carlos de; Perecin,Dilermando.
The embryogenic capability of five soybean cultivars (Renascença, IAS-5, IAC-17, BR-16 and FT-Cometa) was studied at different auxin concentrations (8, 10 and 12 mg/l naphthalene acetic acid, NAA), at different pHs (5.8 and 7.0) and at low (8-12 muEm-2 s-1) and high (27-33 mEm-2 s-1) light intensities. The experimental design was completely randomized with four replications. Immature cotyledons 4-6 mm in length were placed in the six induction mediums evaluated and submitted to two light intensities. Twenty immature cotyledons per cultivar were placed on each Petri dish, which was considered to be one replication. The number of somatic embryos per treatment per replication was counted. The results showed genotype influence on somatic embryogenic capability...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400027

Erratum: Decreased serum PON1 arylesterase activity in familial hypercholesterolemia patients with a mutated LDLR gene
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000316101

Cytogenetics of three Brazilian species of Eleutherodactylus (Anura, Leptodactylidae) with 22 chromosomes and re-analysis of multiple translocations in E. binotatus
Provedor de dados: Genetics and Molecular Biology
Autores: Siqueira-Jr.,Sérgio; Ananias,Fernando; Recco-Pimentel,Shirlei M.
In this paper, we provide a cytogenetic analysis of Eleutherodactylus guentheri, E. parvus and E. binotatus. All of the species had a diploid chromosomal number of 2n = 22. The karyotypes of E. guentheri and E. parvus were very similar and differed only slightly in the morphology of pair 2. These two species also had an NOR-bearing secondary constriction on the long arms of pair 6. The karyotype of E. binotatus differed from those of E. guentheri and E. parvus in the morphology and size of the chromosomes, in the number of chromosomal arms, in the NOR location (detected on the short arms of pair 1), and in the pattern of heterochromatin. These results reinforce the differences between E. guentheri and E. binotatus and support the existence of two species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eleutherodactylus; Heterochromatin; Karyotype; Nucleolus organizing region (NOR); Reciprocal translocation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300010

Factorial analysis of bootstrap variances of population genetic parameter estimates
Provedor de dados: Genetics and Molecular Biology
Autores: Carlini-Garcia,Luciana Aparecida; Vencovsky,Roland; Coelho,Alexandre Siqueira Guedes.
We presented an alternative way to verify the relative contribution to the total variance, of the sources of variation due to populations (P), individuals within populations (I), the (P*I) interaction, and the standard error of the following parameter estimates: total (F) and intrapopulation (f) fixation indices, and divergence among populations (q). The knowledge of this relative contribution is important to establish sampling strategies of natural populations. To attain these objectives, the bootstrap method was used to resample simultaneously populations and individuals, considering different combinations of P and I. This procedure was repeated five times for a given combination of each analyzed data set. For each data set, five estimates of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population structure; Resampling; Natural populations.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200019

Allele frequency and selection efficiency in cross populations of Andean x Mesoamerican common beans (Phaseolus vulgaris L. Fabales, Fabaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Baldoni,Aisy B.; Ramalho,Magno A. Patto; Abreu,Ângela de Fátima B.
Strategies were investigated for improving efficiency in the use of segregating common bean (Phaseolus vulgaris) populations using crosses between the Andean cultivar BRS-Radiante and the Mesoamerican parent cultivar Carioca-MG by developing populations with 12.5%, 25%, 50%, 75% and 87.5% of the allele frequency of one of the parents. For each of the five populations we evaluated for two traits, the number of days to the beginning of flowering and grain yield (g plot-1), in the F2:3 (sown in February 2006) and F2:4 (sown in July 2006) generation progenies using 15 x 15 lattice design experiments, with 44 progenies (n = 220 plants) plus the two parents and three controls being evaluated for each generation. In terms of variability release, the populations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Backcross; Gene pools; Heritability; Phaseolus vulgaris; Selection gain.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500019

Multilocus DNA-fingerprinting using oligonucleotide probes (GATA)4 and (GGAT)4 in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: Genetics and Molecular Biology
Autores: Vergara,N.; Iturra,P.; Aguirre,.
Oligonucleotide probes (GATA)4 and (GGAT)4 for minisatellite loci produced DNA fingerprinting patterns which are unique in individual rainbow trout. These patterns can be used to monitor the efficiency of manipulation of production characters in gynogenetic individuals. The (GATA)4 probe produced highly individual patterns that differentiated individuals among gynogenetic offspring, and could be easily scored.
Tipo: Info:eu-repo/semantics/other
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200011

Errata
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100021

Similar interstitial deletions of the KAL-1 gene in two Brazilian families with X-linked Kallmann Syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Trarbach,Ericka Barbosa; Monlleo,Isabella Lopes; Porciuncula,Carlos Guilherme Gaelzer; Fontes,Marshall Italo Barros; Baptista,Maria Teresa Mathias; Hackel,Christine.
Mutations in the KAL-1 gene localized at Xp22.3 have been shown to be responsible for the X-linked Kallmann syndrome (KS), a disorder characterized by the association of hypogonadotropic hypogonadism and anosmia. In this paper, we describe the investigation of two families with X-linked KS, in which similar interstitial deletions ning exons 5 to 10 of the KAL-1 gene were identified. The presence of interspersed repetitive DNA sequences within the KAL-1 gene might have predisposed to this type of mutation.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: X-linked KS; Intragenic deletions; KAL-1 gene; PCR; Phenotypic variability.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300006

Genotype-environment interaction and phenotypic stability for girth growth and rubber yield of Hevea clones in São Paulo State, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Paulo de Souza; Bortoletto,Nelson; Martins,Antonio Lúcio Mello; Costa,Reginaldo Brito da; Gallo,Paulo Boller.
The best-yielding, best vigour and most stable Hevea clones are identified by growing clones in different environments. However, research on the stability in Hevea brasiliensis (Willd. Adr. ex Juss.) Muell.-Arg. is scarce. The objectives of this work were to assess genotype-environment interaction and determine stable genotypes. Stability analysis were performed on results for girth growth and rubber yield of seven clones from five comparative trials conducted over 10 years (girth growth) and four years (rubber yield) in São Paulo State, Brazil. Stability was estimated using the Eberhart and Russell (1966) method. Year by location and location variability were the dominant sources of interactions. The stability analysis identified GT 1 and IAN 873 as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hevea brasiliensis; Rubber tree; Coefficient of determination; Stability parameters.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400007

A phylogenomic appraisal of the evolutionary relationship of mycoplasmas
Provedor de dados: Genetics and Molecular Biology
Autores: Yotoko,Karla S.C.; Bonatto,Sandro L.
Several genomes of mycoplasmas have been sequenced and here we tried to retrieve the evolutionary relationships of nine species using a phylogenomic approach. Several methods were used to build phylogenetic trees based on protein sequence information, gene-order, and gene-content. We also utilized datasets composed of individual and concatenated sets of orthologous proteins, as well as with reduced unreliable alignment regions. Most of our results converge to a single topology, except for the trees built with both the maximum parsimony method and with the gene-order dataset. The gene-content dataset presented trees consistent with most nodes of the convergent tree, but in the gene-order dataset most internal branches were clearly saturated and unreliable....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogenomic; Mycoplasma; Phylogenetic methods; Maximum likelihood; Maximum parsimony.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200015

Mitochondrial DNA (mtDNA) haplogroups in 1526 unrelated individuals from 11 Departments of Colombia
Provedor de dados: Genetics and Molecular Biology
Autores: Yunis,Juan J.; Yunis,Emilio J.
The frequencies of four mitochondrial Native American DNA haplogroups were determined in 1526 unrelated individuals from 11 Departments of Colombia and compared to the frequencies previously obtained for Amerindian and Afro-Colombian populations. Amerindian mtDNA haplogroups ranged from 74% to 97%. The lowest frequencies were found in Departments on the Caribbean coast and in the Pacific region, where the frequency of Afro-Colombians is higher, while the highest mtDNA Amerindian haplogroup frequencies were found in Departments that historically have a strong Amerindian heritage. Interestingly, all four mtDNA haplogroups were found in all Departments, in contrast to the complete absence of haplogroup D and high frequencies of haplogroup A in Amerindian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Admixture; Colombia; Haplogroup; Mestizo; Mitochondrial DNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300005

Optimization procedures for establishing reserve networks for biodiversity conservation taking into account population genetic structure
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz Filho,José Alexandre Felizola; Telles,Mariana Pires de Campos.
Conservation genetics has been focused on the ecological and evolutionary persistence of targets (species or other intraspecific units), especially when dealing with narrow-ranged species, and no generalized solution regarding the problem of where to concentrate conservation efforts for multiple genetic targets has yet been achieved. Broadly distributed and abundant species allow the identification of evolutionary significant units, management units, phylogeographical units or other spatial patterns in genetic variability, including those generated by effects of habitat fragmentation caused by human activities. However, these genetic units are rarely considered as priority conservation targets in regional conservation planning procedures. In this paper, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity conservation; Population structure; Geographic genetics.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200004

Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes
Provedor de dados: Genetics and Molecular Biology
Autores: Djelic,Ninoslav; Djelic,Dijana; Spremo-Potparevic,Biljana; Zivkovic,Lada; Markovic,Biljana; Lozance,Olivera; Blagojevic,Milos.
Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 µM to 50 µM of L-thyroxine for 24 h and 48 h, these concentrations being...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroxine; Human lymphocytes; Chromosome aberrations; Mitotic index; ROS.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600019

Expression levels of candidate genes for intramuscular fat deposition in two Banna mini-pig inbred lines divergently selected for fatness traits
Provedor de dados: Genetics and Molecular Biology
Autores: Zhao,Su-Mei; Li,Wei-Zhen; Pan,Hong-Bin; Huang,Ying; Yang,Ming-Hua; Wei,Hong-Jiang; Gao,Shi-Zheng.
Intramuscular fat (IMF) content plays an important role in meat quality. Many genes involved in lipid and energy metabolism were identified as candidate genes for IMF deposition, since genetic polymorphisms within these genes were associated with IMF content. However, there is less information on the expression levels of these genes in the muscle tissue. This study aimed at investigating the expression levels of sterol regulating element binding protein-1c (SREBP-1c), diacylglycerol acyltransferase (DGAT-1), heart-fatty acids binding protein (H-FABP), leptin receptor (LEPR) and melanocortin 4 receptor (MC4R) genes and proteins in two divergent Banna mini-pig inbred lines (BMIL). A similar growth performance was found in both the fat and the lean BMIL. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intramuscular fat (IMF); Expression levels; Banna mini-pig inbred line (BMIL).
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500012

Diversification of Ramphastinae (Aves, Ramphastidae) prior to the Cretaceous/Tertiary boundary as shown by molecular clock of mtDNA sequences
Provedor de dados: Genetics and Molecular Biology
Autores: Nahum,Laila Alves; Pereira,Sérgio Luiz; Campos Fernandes,Flora Maria de; Russo Matioli,Sergio; Wajntal,Anita.
Partial cytochrome b and 12S rDNA mitochondrial DNA sequences of eight representatives of the Ramphastidae family were analyzed. We applied the linearized tree method to identify sequences evolving at similar rates and estimated the divergence times among some of the taxa analyzed. After excluding Ramphastos tucanus and Capito dayi from our data set, the remaining taxa presented a constant rate of DNA substitution, and branch lengths could be re-estimated with a clock constraint using the maximum likelihood method. Branch lengths were calibrated assuming that Galliformes and Piciformes split around 100 million years ago (mya). Our results indicate that Ramphastinae, and probably Capitoninae, diverged from other Piciformes in the Late Cretaceous (~82 mya),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Divergence times; K/T; Mass extinction; Cytochrome b; 12S rDNA; Paleogeography; Phylogeny.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400003

Karyological geographic variation of Oligoryzomys nigripes Olfers, 1818 (Rodentia, Cricetidae) from Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Paresque,Roberta; Silva,Maria José de Jesus; Yonenaga-Yassuda,Yatiyo; Fagundes,Valéria.
The karyotypes of 85 specimens of Oligoryzomys nigripes (Rodentia, Sigmodontinae) collected in the Cerrado and Atlantic Forest of seven states of Brazil were analyzed. Eighty four specimens presented a karyotype with 2n = 62 and one individual had 2n = 61 due to a monosomy of the X chromosome. High levels of intra- and inter-population karyotypic variability, due to sex chromosomes heteromorphisms and pericentric inversions in four autosomes (pairs 2, 3, 4 and 8), led to a variation of the autosomal arm numbers (fundamental number, FN) from 78 to 82. Synaptonemal complex analyses revealed normal meiosis in males heterozygous for pericentric inversions. We found 39 different cytotypes, 27 of which are herein described for the first time. A literature survey...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pericentric inversion; Oryzomyini; Oligoryzomys nigripes; Rodentia; Clinal variation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100010

Estimation of variances of the effects of incomplete diallels using a matrix approach
Provedor de dados: Genetics and Molecular Biology
Autores: Assis,Giselle Mariano Lessa de; Fonseca,Ricardo da; Cruz,Cosme Damião; Carneiro Júnior,José Marques.
A matrix approach is described for assessing the variance of effects in incomplete diallels designs. The method is illustrated by reference to simulated complete and incomplete diallels using different combinations of constraints, average degree of dominance and, for the incomplete diallel, number of hybrids. Our results showed that caution should be taken in working with incomplete diallels under conditions of overdominance because there were changes in the rank of the genotypes when the excluded hybrid had parents with a low frequency of the favorable allele (i.e. the allele which increases expression of a character). The expression described in this paper is a rapid and safe approach to estimate variances and covariances of the effects of contrasts of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Incomplete diallel; General combining ability; Specific combining ability.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300017

β-casein gene polymorphism permits identification of bovine milk mixed with bubaline milk in mozzarella cheese
Provedor de dados: Genetics and Molecular Biology
Autores: Otaviano,Antonio R.; Lima,André Luis F.; Laureano,Monyka M.M.; Sena,Janete A.D.; Albuquerque,Lúcia G. de; Tonhati,Humberto.
Mozzarella cheese is traditionally prepared from bubaline (Bubalus bubalis) milk, but product adulteration occurs mainly by addition of or full substitution by bovine milk. The aim of this study was to show the usefulnes of molecular markers to identify the admixture of bovine milk to bubaline milk during the manufacturing process of mozzarella cheese. Samples of mozzarella cheese were produced by adding seven different concentrations of bovine milk: 0%, 1%, 2%, 5%, 8%, 12% and 100%. DNA extracted from somatic cells found in cheese were submitted to PCR-RFLP analysis of casein genes: α-s1-CN - CSN1S1 that encompasses 954 bp from exon VII to intron IX (Alu I and Hinf I), β-CN - CSN2 including 495 bp of exon VII (Hae III and Hinf I), and κ-CN - CSN3,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Casein; Gene polymorphism; Mozarella; Cheese authentication; PCR-RFLP.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500017

Cytochrome P450c17alpha (CYP17) gene polymorphism is not associated with leiomyoma susceptibility
Provedor de dados: Genetics and Molecular Biology
Autores: Yao-Yuan,Hsieh; Fuu-Jen,Tsai; Chi-Chen,Chang; Chang-Hai,Tsai; Cheng-Chieh,Lin; Lian-Shun,Yeh.
Estrogen plays a role in the pathogenesis of leiomyoma. The CYP17 gene codes for the cytochrome P450c17alpha enzyme, which is involved in the biosynthesis of estrogen. Our aim was to investigate if CYP17 polymorphism could be a useful marker to predict the susceptibility to leiomyoma. Our sample of female subjects was divided into two groups: (1) with leiomyoma (n = 159); (2) without leiomyoma (n = 128). A 169-bp fragment encompassing the A1/A2 polymorphic site of the CYP17 gene was amplified by polymerase chain reaction (PCR), restricted by enzyme MspA1I and electrophored on agarose gel. Genotypes and allelic frequencies for this polymorphism in both groups were compared. There was no significant difference between the two groups regarding the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytochrome P450c17; CYP17; Leiomyoma; Single nucleotide polymorphism.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400002

Estimating dispersal and gene flow in the neotropical freshwater turtle Hydromedusa maximiliani (Chelidae) by combining ecological and genetic methods
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Franco L.; Cunha,Anderson F.; Oliveira,Marcos A.; Pereira,Gonçalo A.G.; Reis,Sérgio F. dos.
Hydromedusa maximiliani is a vulnerable neotropical freshwater turtle endemic to mountainous regions of the Atlantic rainforest in southeastern Brazil. Random amplified polymorphic DNA (RAPD) was used to estimate the gene flow and dispersal for individuals inhabiting rivers and streams within a drainage. Nine primers generated 27 scoreable bands, of which 9 (33%) were polymorphic and produced 12 RAPD phenotypes. The gene flow estimates (Nm) among turtles inhabiting different rivers and streams were variable, ranging from 0.09 to 3.00 (mean: 0.60). For some loci, the rates of gene flow could offset population differentiation (Nm &gt; 1), whereas for others random genetic drift could result in population divergence (Nm &lt; 1). Since the genetic variation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydromedusa maximiliani; Dispersal; Gene flow; Conservation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200007

Identification of common bean alleles resistant to anthracnose using RAPD
Provedor de dados: Genetics and Molecular Biology
Autores: Castanheira,Ana L.M.; Santos,João B. dos; Ferreira,Daniel F.; Melo,Leonardo C.
RAPD markers were identified close to common bean alleles responsible for resistance to the fungus Colletotrichum lindemuthianum and may be useful in selecting plants resistant to this pathogen. DNA from F2 plants of the crosses Carioca 300V x P45, Carioca 300V x Ouro and P24 x Ouro was amplified by RAPD. Line P45 has the Co.4 allele for resistance, and the Ouro cultivar has the Co.5 allele. The primer OPC08 amplified a DNA fragment of about 1059 bp linked to the Co.4 allele. The recombination frequency was 0.133 (SE = 0.039; 95% CI = 0.056-0.211). Using the primer OPF10 a DNA fragment of about 912 bp was amplified and found to be associated with the Co.5 allele. The recombination frequency was 0.115 (SE = 0.038; 95% CI = 0.041-0.189). A second marker...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400017

Allozyme comparison of two populations of Rineloricaria (Siluriformes, Loricariidae) from the Ivaí River, upper Paraná River basin, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Limeira,Daniel M.; Renesto,Erasmo; Zawadzki,Cláudio H.
Two allopatric morphotypes of the genus Rinelocaria were compared through the allozyme electrophoresis technique: one morphotype, R. pentamaculata, from the Keller River in the middle stretch of the Ivaí River basin and the other, R. aff. pentamaculata, from the São João River in the upper portion of the Ivaí River basin. The morphotype from the São João River was collected upstream from the São João waterfall, which is about 80 m deep. Twelve enzymatic systems (AAT, ADH, EST, GCDH, G3PDH, GPI, IDH, LDH, MDH, ME, PGM and SOD) were analyzed, which allowed to score 22 loci. Only loci Aat-2, Est-3 and Mdh-C showed polymorphism. The two samples differed in allele frequencies at the three polymorphic loci. The average expected heterozygosity for all loci was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Allozymes; Fish genetics; Genetic distance; Polymorphism; Loricariidae; Pisces.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200034

An analysis of the spontaneous mutation rate measurement in filamentous fungi
Provedor de dados: Genetics and Molecular Biology
Autores: Baracho,Marta S.; Baracho,Ivanhoé R.
Mutations related to gene methG1 of Aspergillus nidulans were analyzed, in order to study a mathematical model for the determination of the mutation rate per nucleus per generation, in filamentous fungi. A replica plating technique was used to inoculate, in a single operation, 26 colonies of the strain, into Petri dishes containing culture medium, and the nine central colonies were analyzed for size and number of conidia in each colony. Using this technique, several central colonies were analyzed with regard to the appearance of mutation, and the number and type of reversions were determined for each colony. The frequencies obtained for each reversion were analyzed, in order to verify if their distribution was in accordance with that of Greenwood and Yule....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus nidulans; Mutation rate; Spontaneous mutation; Fungi; Filamentous fungi.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100014

Variations of protein profiles and calcium and phospholipase A2 concentrations in thawed bovine semen and their relation to acrosome reaction
Provedor de dados: Genetics and Molecular Biology
Autores: Marques,V. Alonso; Goulart,L.R.; Silva,A.E.D. Feliciano.
Just as calcium plays an integral role in acrosome capacitation and reaction, several spermatozoon proteins have been reported as binding to the ovum at fertilization. We examined the relationship between thawed bovine semen protein profiles, seminal plasma calcium ion concentration, spermatozoon phospholipase A2 (PLA2) activity and acrosome reaction. Electrophoretic profile analysis of spermatozoa and bovine seminal plasma proteins (total and membrane) revealed qualitative and quantitative differences among bulls. Variations in PLA2 and seminal plasma calcium concentration indicated genetic diversity among individuals. A 15.7-kDa membrane protein was significantly correlated (r = 0.71) with acrosome reaction, which in turn has been associated with in vivo...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400020

The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
Provedor de dados: Genetics and Molecular Biology
Autores: Mansouri,Vahid; Salehi,Mohammad; Nourozian,Mohsen; Fadaei,Fatemeh; Farahani,Reza Mastery; Piryaei,Abbas; Delbari,Ali.
Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no immunological disorders. Although ntESCs are able to differentiate into somatic cells, the ability of ntESCs to differentiate into primordial germ cells (PGCs) has not been examined. In this work, we examined the capacity of mouse ntESCs to differentiate into PGCs in vitro. ntESCs aggregated to form embryoid bodies (EB) in EB culture medium supplemented with bone morphogenetic protein 4(BMP4) as the differentiation factor. The expression level of specific PGC genes was compared at days 4 and 8 using real time PCR. Flow cytometry and immunocytochemical staining were used to detect Mvh as a specific PGC marker. ntESCs expressed particular genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Differentiation; Germ cells; Nuclear transfer embryonic stem cells.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200220

Can WRKY transcription factors help plants to overcome environmental challenges?
Provedor de dados: Genetics and Molecular Biology
Autores: Finatto,Taciane; Viana,Vívian Ebeling; Woyann,Leomar Guilherme; Busanello,Carlos; Maia,Luciano Carlos da; Oliveira,Antonio Costa de.
Abstract WRKY transcription factors (TFs) are responsible for the regulation of genes responsive to many plant growth and developmental cues, as well as to biotic and abiotic stresses. The modulation of gene expression by WRKY proteins primarily occurs by DNA binding at specific cis-regulatory elements, the W-box elements, which are short sequences located in the promoter region of certain genes. In addition, their action can occur through interaction with other TFs and the cellular transcription machinery. The current genome sequences available reveal a relatively large number of WRKY genes, reaching hundreds of copies. Recently, functional genomics studies in model plants have enabled the identification of function and mechanism of action of several WRKY...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transcriptional regulation; Signaling; Abscisic acid; Kinases; Stresses.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400533

Identification of a RAPD marker linked to the Co-6 anthracnose resistant gene in common bean cultivar AB 136
Provedor de dados: Genetics and Molecular Biology
Autores: Alzate-Marin,Ana Lilia; Menarim,Henrique; Chagas,José Mauro; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves.
The pathogenic variability of the fungus Colletotrichum lindemuthianum represents an obstacle for the creation of resistant common bean (Phaseolus vulgaris L.) varieties. Gene pyramiding is an alternative strategy for the development of varieties with durable resistance. RAPD markers have been proposed as a means to facilitate pyramiding of resistance genes without the need for multiple inoculations of the pathogens. The main aims of this work were to define the inheritance pattern of resistance present in common bean cultivar AB 136 in segregating populations derived from crosses with cultivar Rudá (susceptible to most C. lindemuthianum races) and to identify RAPD markers linked to anthracnose resistance. The two progenitors, populations F1 and F2, F2:3...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300023

Chromosome mapping of ribosomal genes and histone H4 in the genus Radacridium (Romaleidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Anjos,Allison; Loreto,Vilma; Souza,Maria José de.
In this study, two species of Romaleidae grasshoppers, Radacridium mariajoseae and R.nordestinum, were analyzed after CMA3/DA/DAPI sequential staining and fluorescence in situ hybridization (FISH) to determine the location of the 18S and 5S rDNA and histone H4 genes. Both species presented karyotypes composed of 2n = 23, X0 with exclusively acrocentric chromosomes. CMA3+ blocks were detected after CMA3/DA/DAPI staining in only one medium size autosome bivalent and in the X chromosome in R. mariajoseae. On the other hand, all chromosomes, except the L1 bivalent, of R. nordestinum presented CMA3+ blocks. FISH analysis showed that the 18S genes are restricted to the X chromosome in R. mariajoseae, whereas these genes were located in the L2,S9 and S10...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; FISH; Fluorochromes; Grasshoppers; RDNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300006

Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Yaish,M.W.F.; Pérez de la Vega,M.
The isolation of (GA)n microsatellites using a highly microsatellite-enriched library is described for the first time in common bean (Phaseolus vulgaris L.). A relatively simple and effective method to isolate DNA repeats from microsatellite-enriched libraries based on hybridization-capture of repeat regions using biotin-conjugated oligonucleotids and non-radioactive colony hybridization was carried out. PCR products from 200 to 800 bp were obtained and cloned. Of the 60 clones sequenced, 21 yielded (GA)n microsatellites with n values equal or higher than six. These (GA)n microsatellite-containing loci could be useful for further genetic mapping studies. A (GA)n microsatellite linked to a putative MADS-box gene was identified. This sequence, which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris L.; Genetic markers; Microsatellites; MADS-box; Transcriptional factor.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300019

Predicting performance of soybean populations using genetic distances estimated with RAPD markers
Provedor de dados: Genetics and Molecular Biology
Autores: Barroso,Paulo Augusto Vianna; Geraldi,Isaias Olívio; Vieira,Maria Lúcia Carneiro; Pulcinelli,Carlos Eduardo; Vencovsky,Roland; Dias,Carlos Tadeu dos Santos.
In order to verify whether genetic distance (GD) is associated with population mean (PM), genetic variance (GV) and the proportion of superior progenies generated by each cross in advanced generations of selfing (PS), the genetic distances between eight soybean lines (five adapted and three non-adapted) were estimated using 213 polymorphic RAPD markers. The genetic distances were partitioned according to Griffing's Model I Method 4 for diallel analysis, i.e., GDij = GD+ GGDi+ GGDj + SGDij. Phenotypic data were recorded for seed yield and plant height for 25 out of 28 populations of a diallel set derived from the eight soybean lines and evaluated from F2:8 to F2:11 generations. No significant correlation for seed yield was detected between GD and GV, while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean; Genetic distance; Molecular markers; RAPD; Prediction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300020

Polymorphism in the promoter region of von Willebrand factor gene and von Willebrand disease type 1
Provedor de dados: Genetics and Molecular Biology
Autores: Simon,Daniel; Bandinelli,Eliane; Roisenberg,Israel.
The -1185A/G polymorphism in the 5'-regulatory region of the von Willebrand factor (VWF) gene was associated with VWF plasma levels in a normal population. This study was undertaken to evaluate whether there is a relationship between this polymorphism and type 1 von Willebrand disease (VWD), a disorder characterized by a quantitative deficiency of VWF. The association between this polymorphism and plasma VWF levels in normal Brazilian individuals was also analyzed. Control subjects (n = 460) and type 1 VWD patients (n = 41) were studied. Polymerase chain reaction (PCR) amplification of the 864-bp VWF promoter region followed by AccII restriction-digestion was used to identify the -1185A/G genotypes. The -1185G allele frequency was 57% in normal individuals...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Von Willebrand factor; Promoter polymorphisms; Genetics; Von Willebrand disease.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400001

Is a non-synonymous SNP in the HvAACT1 coding region associated with acidic soil tolerance in barley?
Provedor de dados: Genetics and Molecular Biology
Autores: Ferreira,Jéssica Rosset; Faria,Bruna Franciele; Comar Junior,Moacyr; Delatorre,Carla Andréa; Minella,Euclydes; Pereira,Jorge Fernando.
Abstract The barley HvAACT1 gene codes for a citrate transporter associated with tolerance to acidic soil. In this report, we describe a single nucleotide polymorphism (SNP) in the HvAACT1 coding region that was detected as T-1,198 (in genotypes with lower root growth on acidic soil) or G-1,198 (greater root growth) and resulted in a single amino acid change (L/V-172). Molecular dynamic analysis predicted that HvAACT1 proteins with L or V-172 were stable, although the substitution led to structural changes within the protein. To evaluate the effect of the SNP on tolerance to acidic soil, barley accessions were separated into haplotypes based on the presence of a 1 kb insertion in the HvAACT1 promoter and a 21 bp insertion/deletion. These markers and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aluminium tolerance; Citrate transporter; Haplotype; Hordeum vulgare; Single nucleotide polymorphism.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300480

Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus
Provedor de dados: Genetics and Molecular Biology
Autores: Evangelista,Cláudia Carolina Silva; Guidelli,Giovanna Vieira; Borges,Gustavo; Araujo,Thais Fenz; Souza,Tiago Alves Jorge de; Neves,Ubiraci Pereira da Costa; Tunnacliffe,Alan; Pereira,Tiago Campos.
Abstract The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holo-anhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involved in oxidative stress tolerance, while a novel medium-scale RNAi workflow identified a further 40 anhydrobiosis-associated genes, including several involved in proteostasis, DNA repair and signal transduction pathways. This suggests that multiple genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peroxiredoxin; Kinase; RNAi; Proteostasis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500790

Characterization of beta-thalassemia mutations in patients from the state of Rio Grande do Norte, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Silveira,Zama Messala Luna da; Barbosa,Maria das Vitórias; Fernandes,Thales Allyrio Araújo de Medeiros; Kimura,Elza Miyuki; Costa,Fernando Ferreira; Sonati,Maria de Fátima; Rebecchi,Ivanise Marina Moretti; Medeiros,Tereza Maria Dantas de.
35 unrelated individuals were studied for characterization as either heterozygous or homozygous for beta-thalassemia. Molecular analysis was done by PCR/RFLP to detect the mutations most commonly associated with beta-thalassemia (β0IVS-I-1, β+IVS-I-6, and β039). In the patients who showed none of these mutations, the beta-globin genes were sequenced. Of the 31 heterozygous patients, 13 (41.9%) had the β+IVS-I-6 mutation, 15 (48.4%) the β0IVS-I-1 mutation, 2 (6.5%) the β+IVS-I-110 mutation and 1 (3.2%) the β+IVS-I-5 mutation. IVS-I-6 was detected in the four homozygotes. The mutation in codon 39, often found in previous studies in Brazil, was not detected in the present case. This is the first study aiming at identifying mutations that determine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hereditary hemoglobinopathies; Beta-thalassemia; Mutations; PCR-RFLP; Brazilian population.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300010

Genome-wide comparison of genes involved in the biosynthesis, metabolism, and signaling of juvenile hormone between silkworm and other insects
Provedor de dados: Genetics and Molecular Biology
Autores: Cheng,Daojun; Meng,Meng; Peng,Jian; Qian,Wenliang; Kang,Lixia; Xia,Qingyou.
Juvenile hormone (JH) contributes to the regulation of larval molting and metamorphosis in insects. Herein, we comprehensively identified 55 genes involved in JH biosynthesis, metabolism and signaling in the silkworm (Bombyx mori) as well as 35 in Drosophila melanogaster, 35 in Anopheles gambiae, 36 in Apis mellifera, 47 in Tribolium castaneum, and 44 in Danaus plexippus. Comparative analysis showed that each gene involved in the early steps of the mevalonate (MVA) pathway, in the neuropeptide regulation of JH biosynthesis, or in JH signaling is a single copy in B. mori and other surveyed insects, indicating that these JH-related pathways or steps are likely conserved in all surveyed insects. However, each gene participating in the isoprenoid branch of JH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Juvenile hormone; Biosynthesis; Metabolism; Signaling; Gene duplication.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300018

Genome-wide identification and phylogenetic analysis of the ERF gene family in cucumbers
Provedor de dados: Genetics and Molecular Biology
Autores: Hu,Lifang; Liu,Shiqiang.
Members of the ERF transcription-factor family participate in a number of biological processes, viz., responses to hormones, adaptation to biotic and abiotic stress, metabolism regulation, beneficial symbiotic interactions, cell differentiation and developmental processes. So far, no tissue-expression profile of any cucumber ERF protein has been reported in detail. Recent completion of the cucumber full-genome sequence has come to facilitate, not only genome-wide analysis of ERF family members in cucumbers themselves, but also a comparative analysis with those in Arabidopsis and rice. In this study, 103 hypothetical ERF family genes in the cucumber genome were identified, phylogenetic analysis indicating their classification into 10 groups, designated I to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cucumis sativus L.; ERF; Phylogenetic analysis; Transcription factor; Genome sequence.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400015

Karyotype and genome size of Iberochondrostoma almacai (Teleostei, Cyprinidae) and comparison with the sister-species I. lusitanicum
Provedor de dados: Genetics and Molecular Biology
Autores: Monteiro,Rita; Carvalho,Cláudia; Collares-Pereira,Maria João.
This study aimed to define the karyotype of the recently described Iberian endemic Iberochondrostoma almacai, to revisit the previously documented chromosome polymorphisms of its sister species I. lusitanicum using C-, Ag-/CMA3 and RE-banding, and to compare the two species genome sizes. A 2n = 50 karyotype (with the exception of a triploid I. lusitanicum specimen) and a corresponding haploid chromosome formula of 7M:15SM:3A (FN = 94) were found. Multiple NORs were observed in both species (in two submetacentric chromosome pairs, one of them clearly homologous) and a higher intra and interpopulational variability was evidenced in I. lusitanicum. Flow cytometry measurements of nuclear DNA content showed some significant differences in genome size both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ag/CMA3-banding; Flow cytometry; NORs; Chromosome polymorphism; Spontaneous triploidy.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200011

Chromosomal damage in air crew members of international flights: A preliminary report
Provedor de dados: Genetics and Molecular Biology
Autores: Picco,S.J.; De Luca,J.C.; MacIntyre,C.; Dulout,F.N.
Air crew members of international flights are exposed to ionizing radiations originated from the collision of cosmic particles with atoms of the atmosphere. The degree of exposure varies according to the altitude, latitude, and solar activity. The cytogenetical analysis showed a significant increase of dicentric chromosomes (14.5 ± 0.76 per 1,000 cells) in active air crew members as well as in retired air crew members (7.5 ± 0.59 per 1,000 cells) in relation to controls (0.5 ± 0.05 per 1,000 cells). In active members the frequency of ring chromosomes was also increased (2.0 ± 0.31 per 1,000 cells) but not found in retired crew members. These observations suggest a high degree of exposure to ionizing radiations of air crew personnel. Consequently, the...
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400061

Embryo sac development in yellow passion fruit Passiflora edulis f. flavicarpa (Passifloraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Margarete Magalhães de; Pereira,Telma Nair Santana; Hoffmann,Magali; Melo,Edésio J.T. de; Louro,Ricardo Pereira.
The yellow passion fruit, Passiflora edulis f. flavicarpa, is one of the most important Brazilian fruit crops. It is an allogamous, diploid, and self-incompatible species. It has hermaphrodite, solitary flowers, located in the leaf axils and protected by leaf bracts. The flower has an androgynophore, which is a straight stalk supporting its reproductive parts. There are usually five anthers, located at the tip of each of the five filaments. The ovary is borne just above the filaments, at the top of the androgynophore; there are three styles that are united at their base, and at the top there are three stigmas. The objective of this research was to observe embryo sac development in yellow passion flowers. Ovaries at different stages of development were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Egg cell; Embryo sac; Passifloraceae; Yellow passion fruit; Passiflora edulis f. flavicarpa Deg.; Confocal microscopy; Megagametogenesis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400017

Phylogenetic relationships in the Drosophila fasciola species subgroup (Diptera, Drosophilidae) inferred from partial sequences of the mitochondrial cytochrome oxidase subunit I (COI) gene
Provedor de dados: Genetics and Molecular Biology
Autores: Silva-Bernardi,Erica C.C.; Morales,Adriana C.; Sene,Fabio M.; Manfrin,Maura H.
The repleta group is one of the largest and most complex species groups in the genus Drosophila, representing an important biological model for the elaboration of evolutionary and biogeographical hypotheses on the American Continent. It is divided into six subgroups, of which the fasciola subgroup is the only one with most of its species found in the humid forests of Central and South America. With the aim of understanding the origin and evolution of the fasciola subgroup, and consequently adding information about the repleta group, we analyzed partial sequences of the mitochondrial cytochrome oxidase subunit I (COI) gene of nine Drosophila species (D. carolinae, D. coroica, D. ellisoni, D. fascioloides, D. ivai, D. moju, D. onca, D. rosinae and D. senei)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fasciola subgroup; Molecular phylogeny; MtDNA; Systematics; Repleta group.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300029

RNA editing in the chloroplast of Asian Palmyra palm (Borassus flabellifer)
Provedor de dados: Genetics and Molecular Biology
Autores: Sakulsathaporn,Arpakorn; Wonnapinij,Passorn; Suttangkakul,Anongpat; Apisitwanich,Somsak; Vuttipongchaikij,Supachai.
Abstract We have identified 46 RNA editing sites located in 20 chloroplast (cp) genes of Borassus flabellifer (Asian Palmyra palm), family Arecaceae, and tested these genes for supporting phylogenetic study among the commelinids. Among the 46 sites, 43 sites were found to cause amino acid alterations, which were predicted to increase the hydrophobicity and transmembrane regions of the proteins, and one site was to cause a premature stop codon. Analysis of these editing sites with data obtained from seed plants showed that a number of shared-editing sites depend on the evolutionary relationship between plants. We reconstructed a deep phylogenetic relationship among the commelinids using seven RNA edited genes that are orthologous among monocots. This tree...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arecaceae; Arecales; Chloroplast genome; Commelinids; Phylogeny.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500601

Modeling and analysis of soybean (Glycine max. L) Cu/Zn, Mn and Fe superoxide dismutases
Provedor de dados: Genetics and Molecular Biology
Autores: Ramana Gopavajhula,V.; Viswanatha Chaitanya,K.; Akbar Ali Khan,P.; Shaik,Jilani P.; Narasimha Reddy,P.; Alanazi,Mohammad.
Superoxide dismutase (SOD, EC 1.15.1.1) is an important metal-containing antioxidant enzyme that provides the first line of defense against toxic superoxide radicals by catalyzing their dismutation to oxygen and hydrogen peroxide. SOD is classified into four metalloprotein isoforms, namely, Cu/Zn SOD, Mn SOD, Ni SOD and Fe SOD. The structural models of soybean SOD isoforms have not yet been solved. In this study, we describe structural models for soybean Cu/Zn SOD, Mn SOD and Fe SOD and provide insights into the molecular function of this metal-binding enzyme in improving tolerance to oxidative stress in plants.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amino acid analysis; Model evaluation; Molecular modeling; Phylogenetic analysis; Superoxide dismutase (SOD).
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200013

The mitochondrial genome of the stingless bee Melipona bicolor (Hymenoptera, Apidae, Meliponini): sequence, gene organization and a unique tRNA translocation event conserved across the tribe Meliponini
Provedor de dados: Genetics and Molecular Biology
Autores: Silvestre,Daniela; Dowton,Mark; Arias,Maria Cristina.
At present a complete mtDNA sequence has been reported for only two hymenopterans, the Old World honey bee, Apis mellifera and the sawfly Perga condei. Among the bee group, the tribe Meliponini (stingless bees) has some distinction due to its Pantropical distribution, great number of species and large importance as main pollinators in several ecosystems, including the Brazilian rain forest. However few molecular studies have been conducted on this group of bees and few sequence data from mitochondrial genomes have been described. In this project, we PCR amplified and sequenced 78% of the mitochondrial genome of the stingless bee Melipona bicolor (Apidae, Meliponini). The sequenced region contains all of the 13 mitochondrial protein-coding genes, 18 of 22...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melipona bicolor; Meliponini; Social insects; Mitochondrial genome; Gene rearrangements.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300010

Clinical evaluation of a substitute of HLA-B*58:01 in different Chinese ethnic groups
Provedor de dados: Genetics and Molecular Biology
Autores: Zhang,Xinju; Jin,Lei; Wu,Zhiyuan; Ma,Weizhe; Chen,Yuming; Chen,Gang; Wang,Lixin; Guan,Ming.
Abstract The goal of this research was to investigate the linkage disequilibrium between rs9263726 and HLA-B*58:01 in different Chinese ethnic groups (Han, Tibet, and Hui) and to study the feasibility of rs9263726 replacing HLA-B*58:01 as an efficient indicator of potential allopurinol hypersensitivity syndrome. In this study, rs9263726 and HLA-B*58:01 were detected in all samples. For samples of individuals whose rs9263726 genotypes were not consistent with HLA-B*58:01, we did high-resolution typing of HLA-B gene to further confirm the correlation of rs9263726 genotype and special HLA-B alleles. We confirmed that the linkage disequilibrium between rs9263726 and HLA-B*58:01 was more significant in the Han ethnic group (r2=0.886, D’=1.0) than in the Tibet...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rs9263726; HLA-B*58:01; Allopurinol hypersensitivity reaction; Tag SNP.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400578

Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
Provedor de dados: Genetics and Molecular Biology
Autores: Granada,Camille E; Strochein,Marcos; Vargas,Luciano K; Bruxel,Manuela; Sá,Enilson Luiz Saccol de; Passaglia,Luciane M.P.
This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhizobia; Nodulation; Nitrogen; Specificity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300012

alpha-thalassemia, HbS, and beta-globin gene cluster haplotypes in two Afro-Uruguayan sub-populations from northern and southern Uruguay
Provedor de dados: Genetics and Molecular Biology
Autores: Luz,Julio A. da; Sans,Mónica; Kimura,Elza Miyuki; Albuquerque,Dulcinéia Martins; Sonati,Maria de Fatima; Costa,Fernando Ferreira.
Hemoglobinopathies are the most common monogenic disorders worldwide; however, they have never been systematically studied from a genetic perspective in Uruguay. In this study, we determined the frequencies of hemoglobin variants in Afro-Uruguayans. A sample of 52 healthy unrelated Afro-Uruguayans from the northern (N = 28) and southern (N = 24) regions of the country was analyzed. Eight individuals (15.4%) were heterozygous for -alpha3,7thalassemia; seven of them (29.2%) were originally from the southern region, whereas one of them (3.6%) was from the northern region; the differences between both regions were statistically significant (p = 0.016 +/-0.003). The only structural mutation detected was betaS, which is typical of African populations. Four...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemoglobinopathies; Alpha-thalassemias; HbS; Haplotypes; Afro-derived populations.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400002

In vivo cytogenetic effects of multiple doses of dietary vegetable oils
Provedor de dados: Genetics and Molecular Biology
Autores: Evangelista,Cristina Márcia Wolf; Antunes,Lusânia Maria Greggi; Bianchi,Maria de Lourdes Pires.
Polyphenols are potent antioxidants that are particularly abundant in the Mediterranean diet, with olive oil being the main fat source. A number of investigations have reported that phenolic compounds found in dietary oils are antioxidants and could provide protective effects by inhibiting DNA oxidative damage. However, few studies have been published on the biological activity of vegetable oils, including their possible mutagenic/antimutagenic effects. The objective of the current study was to investigate the cytogenetic effects of multiple doses of four vegetable oils in rat bone marrow cells and to examine the possible antimutagenic effects of these oils in chromosomal damage induced by the antitumor drug cisplatin. These oils are consumed by humans and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimutagenesis; Chromosomal aberrations; Vegetable oils; Wistar rats.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400024

Cytotaxonomy of Parodon nasus and Parodon tortuosus (Pisces, Characiformes): A case of synonymy confirmed by cytogenetic analyses
Provedor de dados: Genetics and Molecular Biology
Autores: Bellafronte,Elisangela; Margarido,Vladimir Pavan; Moreira-Filho,Orlando.
Morphological and cytogenetical studies were carried out on the freshwater fish Parodon nasus and Parodon tortuosus in order to evaluate a putative synonymy. The diploid chromosome number observed in both species was 2n = 54 (48M/SM and 6ST) with no differences between the sexes. Despite slight differences in the pattern of heterochromatin distribution and the number of cusps in symphysean teeth, the location of nucleolar organizer regions (NORs) and 5S rRNA genes (both species-specific features) were similar in both species. The remarkable similarity observed between these allopatric species supports recent taxonomical reviews indicating that P. tortuosus is a synonym for P. nasus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Parodontidae; Systematics; 18S rDNA; 5S rDNA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500010

Sensitivity, advantages, limitations, and clinical utility of targeted next-generation sequencing panels for the diagnosis of selected lysosomal storage disorders
Provedor de dados: Genetics and Molecular Biology
Autores: Málaga,Diana Rojas; Brusius-Facchin,Ana Carolina; Siebert,Marina; Pasqualim,Gabriela; Saraiva-Pereira,Maria Luiza; Souza,Carolina F.M de; Schwartz,Ida V.D.; Matte,Ursula; Giugliani,Roberto.
Abstract Lysosomal storage disorders (LSDs) constitute a heterogeneous group of approximately 50 genetic disorders. LSDs diagnosis is challenging due to variability in phenotype penetrance, similar clinical manifestations, and a high allelic heterogeneity. A powerful tool for the diagnosis of the disease could reduce the “diagnostic odyssey” for affected families, leading to an appropriate genetic counseling and a better outcome for current therapies, since enzyme replacement therapies have been approved in Brazil for Gaucher, Fabry, and Pompe diseases, and are under development for Niemann-Pick Type B. However, application of next-generation sequencing (NGS) technology in the clinical diagnostic setting requires a previous validation phase. Here, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ion Torrent; Molecular diagnostics; Next-generation sequencing; Lysosomal storage disorders; Validation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200197

Bernardo Beiguelman (1932-2010)
Provedor de dados: Genetics and Molecular Biology
Autores: Krieger,Henrique; Pinto Jr,Walter.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200001

Identification of genes related to high royal jelly production in the honey bee (Apis mellifera) using microarray analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Nie,Hongyi; Liu,Xiaoyan; Pan,Jiao; Li,Wenfeng; Li,Zhiguo; Zhang,Shaowu; Chen,Shenglu; Miao,Xiaoqing; Zheng,Nenggan; Su,Songkun.
Abstract China is the largest royal jelly producer and exporter in the world, and high royal jelly-yielding strains have been bred in the country for approximately three decades. However, information on the molecular mechanism underlying high royal jelly production is scarce. Here, a cDNA microarray was used to screen and identify differentially expressed genes (DEGs) to obtain an overview on the changes in gene expression levels between high and low royal jelly producing bees. We developed a honey bee gene chip that covered 11,689 genes, and this chip was hybridised with cDNA generated from RNA isolated from heads of nursing bees. A total of 369 DEGs were identified between high and low royal jelly producing bees. Amongst these DEGs, 201 (54.47%) genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Honeybee; Royal jelly production; Gene chip; Molecular marker; Differentially expressed genes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500781

Genetics of the Pantaneiro horse of the Pantanal region of Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Cothran,E. Gus; Santos,Sandra Aparecida; Mazza,Maria Cristina Medeiros; Lear,Tery L.; Sereno,J. Robson Bezerra.
Genetic variation at seven red blood cell alloantigen, blood group loci and 10 biochemical genetic loci was examined in 102 Pantaneiro horses from the Pantanal region of Brazil and compared to that of other domestic horse breeds. Measures of both individual genic variation and populational genetic diversity within the Pantaneiro were near the average for domestic horse breeds. From the standpoint of genetic conservation there is no immediate concern for loss of variation within the Pantaneiro. Analysis of genetic relationship showed that the Pantaneiro was clearly of Iberian Peninsula descent and that it was most closely related to other Brazilian breeds.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300009

DNA extraction from sea anemone (Cnidaria: Actiniaria) tissues for molecular analyses
Provedor de dados: Genetics and Molecular Biology
Autores: Pinto,S.M.; Fernandes-Matioli,F.M.C.; Schlenz,E.
A specific DNA extraction method for sea anemones is described in which extraction of total DNA from eight species of sea anemones and one species of corallimorpharian was achieved by changing the standard extraction protocols. DNA extraction from sea anemone tissue is made more difficult both by the tissue consistency and the presence of symbiotic zooxanthellae. The technique described here is an efficient way to avoid problems of DNA contamination and obtain large amounts of purified and integral DNA which can be used in different kinds of molecular analyses.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300017

Transcriptome analysis of resistant soybean roots infected by Meloidogyne javanica
Provedor de dados: Genetics and Molecular Biology
Autores: Sá,Maria Eugênia Lisei de; Lopes,Marcus José Conceição; Campos,Magnólia de Araújo; Paiva,Luciano Vilela; Amorim,Regina Maria Santos de; Beneventi,Magda Aparecida; Firmino,Alexandre Augusto Pereira; Sá,Maria Fátima Grossi de.
Soybean is an important crop for Brazilian agribusiness. However, many factors can limit its production, especially root-knot nematode infection. Studies on the mechanisms employed by the resistant soybean genotypes to prevent infection by these nematodes are of great interest for breeders. For these reasons, the aim of this work is to characterize the transcriptome of soybean line PI 595099-Meloidogyne javanica interaction through expression analysis. Two cDNA libraries were obtained using a pool of RNA from PI 595099 uninfected and M. javanica (J2) infected roots, collected at 6, 12, 24, 48, 96, 144 and 192 h after inoculation. Around 800 ESTs (Expressed Sequence Tags) were sequenced and clustered into 195 clusters. In silico subtraction analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; Root knot nematode; Transcriptome.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200008

Genes associated with hypersensitive response (HR) in the citrus EST database (CitEST)
Provedor de dados: Genetics and Molecular Biology
Autores: Guidetti-Gonzalez,Simone; Freitas-Astúa,Juliana; Amaral,Alexandre Morais do; Martins,Natália F.; Mehta,Angela; Silva,Marilia Santos; Carrer,Helaine.
Plants are continuously exposed to pathogen attack, but successful infection is rare because they protect themselves against pathogens using a wide range of response mechanisms. One of them is the hypersensitive response (HR), which is a form of cell death often associated with plant resistance to pathogen infection to prevent the spreadsebpg@cnpq.br sebpg@cnpq.br of the potential pathogen from infected to uninfected tissues. Cell death is activated by recognition of pathogen-derived molecules by the resistance (R) gene products, and is associated with the massive accumulation of reactive oxygen species (ROS), salicylic acid (SA), and other pro-death signals such as nitric oxide (NO). The analysis of the citrus EST (CitEST) database revealed the presence...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Otic and abiotic stress response; Plant-pathogen incompatible interaction; Programmed cell death; Plant disease resistance.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500022

Cytotoxicity and genotoxicity of stilbene derivatives in CHO-K1 and HepG2 cell lines
Provedor de dados: Genetics and Molecular Biology
Autores: Mizuno,Cassia Suemi; Ampomaah,Winnifred; Mendonça,Fernanda Ribeiro; Andrade,Gabriela Carvalho; Silva,Ariel Maria Nazaré da; Goulart,Mirian Oliveira; Santos,Raquel Alves dos.
Abstract The cytotoxicity and genotoxicity of the stilbenes (E)-methyl-4-(3-5-dimethoxystyryl)benzoate (ester), (E)-4-(3-5-dimethoxystyryl)aniline (amino), (Z)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (cis-TMS) and (E)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (trans-TMS) were investigated in this work. Structural modifications of resveratrol, a naturally occurring stilbene, have been previously performed, including the replacement of hydroxyl by different functional groups. Such modifications resulted in significant improvement of target-specific effects on cell death and antiproliferative responses. The parameters were evaluated using XTT assay, clonogenic survival assay and the cytokinesis-block micronucleus assay in CHO-K1 and HepG2 cell lines. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stilbene; Cytotoxicity; Genotoxicity; Micronucleus assay.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400656

The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds
Provedor de dados: Genetics and Molecular Biology
Autores: Zhang,Chunyan; Liu,Yun; Huang,Kunkun; Zeng,Wenbing; Xu,Deqing; Wen,Qunying; Yang,Liguo.
Two active mutations (A 781 G and A 1575 G) in growth hormone (GH) gene, and their associations with litter size (LS), were investigated in both a high prolificacy (Matou, n = 182) and a low prolificacy breed (Boer, n = 352) by using the PCR-RFLP method. Superovulation experiments were designed in 57 dams, in order to evaluate the effect of different genotypes of the GH gene on superovulation response. Two genotypes (AA and AB, CC and CD) in each mutation were detected in these two goat breeds. Neither BB nor DD homozygous genotypes were observed. The genotypic frequencies of AB and CC were significantly higher than those of AA and CD. In the third parity, Matou dams with AB or CC genotypes had significantly larger litter sizes than those with AA and CD (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA polymorphism; Growth hormone (GH) gene; Litter size; Superovulation response; Goat.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100010

Neuroprotective changes in degeneration-related gene expression in the substantia nigra following acupuncture in an MPTP mouse model of Parkinsonism: Microarray analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Yeo,Sujung; An,Keon Sang; Hong,Yeon-Mi; Choi,Yeong-Gon; Rosen,Bruce; Kim,Sung-Hoon; Lim,Sabina.
Parkinson’s disease (PD) is a neurodegenerative disorder characterized by the death of dopamine-generating cells in the substantia nigra (SN). Acupuncture stimulation results in an enhanced survival of dopaminergic neurons in the SN in Parkinsonism animal models. The present study investigated changes in gene expression profiles measured using whole transcript array in the SN region related to the inhibitory effects of acupuncture in a chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) Parkinsonism model. In this model, acupuncture stimulation at GB34 and LR3 attenuated the decrease in tyrosine hydroxylase in the SN region; stimulation at non-acupoints did not suppress this decrease. Gene array analysis revealed that 22 (10 annotated genes: Cdh1,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parkinson’s disease; Gene array; Differentially expressed genes; Neurodegeneration; Acupoints.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100115

Effect of organic tomato ( Lycopersicon esculentum ) extract on the genotoxicity of doxorubicin in the  Drosophila  wing spot test
Provedor de dados: Genetics and Molecular Biology
Autores: Dutra,Elaine S.; Dias,Cristina D.; Araújo,Bethânia C. de; Castro,Antônio J. S.; Nepomuceno,Júlio C.
The consumption of organic tomatoes (ORTs) reduces the risk of harmful effects to humans and the environment caused by exposure to toxic agrochemicals. In this study, we used the somatic mutation and recombination test (SMART) of wing spots in  Drosophila melanogaster  to evaluate the genotoxicity of ORT and the effect of cotreatment with ORT on the genotoxicity of Doxorubicin ®  (DXR, a cancer chemotherapeutic agent) that is mediated by free radical formation. Standard (ST) cross larvae were treated chronically with solutions containing 25%, 50% or 100% of an aqueous extract of ORT, in the absence and presence of DXR (0.125 mg/mL), and the number of mutant spots on the wings of emergent flies was counted. ORT alone was not genotoxic but enhanced the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Co-genotoxicity; Drosophila melanogaster; Organic tomato; SMART.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100020

Comparison of base substitutions in response to nitrogen ion implantation and 60Co-gamma ray irradiation in Escherichia coli
Provedor de dados: Genetics and Molecular Biology
Autores: Chuan-Xiao,Xie; An,Xu; Li-Jun,Wu; Jian-Min,Yao; Jian-Bo,Yang; Zeng-Liang,Yu.
To identify the specificity of base substitutions, a novel experimental system was established based on rifampicin-resistant (Rif r) mutant screening and sequencing of the defined region of the rpoB gene in E. coli. We focused on comparing mutational spectra of base substitutions induced by either low energy nitrogen ion beam implantation or 60Co-gamma rays. The most significant difference in the frequency of specific kinds of mutations induced by low energy nitrogen ion beam was that CG &lt;FONT FACE=Symbol&gt;®&lt;/font&gt;TA transitions were significantly increased from 32 to 46, AT &lt;FONT FACE=Symbol&gt;®&lt;/font&gt;TA transversions were doubled from 7 to 15 in 50 mutants, respectively. The preferential base substitutions induced by nitrogen ion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Base substitution; Low-energy nitrogen ion beam implantation; 60Co-gamma rays; Escherichia coli.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200025

Thesis abstracts
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400022

Genome-wide characterization of the NRAMP gene family in Phaseolus vulgaris provides insights into functional implications during common bean development
Provedor de dados: Genetics and Molecular Biology
Autores: Ishida,Juliane Karine; Caldas,Danielle G.G.; Oliveira,Lucas Roberto; Frederici,Gabriela Campos; Leite,Lucas Margato Pereira; Mui,Tsai Siu.
Abstract Transporter proteins play an essential role in the uptake, trafficking and storage of metals in plant tissues. The Natural Resistance-Associated Macrophage Protein (NRAMP) family plays an essential role in divalent metal transport. We conducted bioinformatics approaches to identify seven NRAMP genes in the Phaseolus vulgaris genome, investigated their phylogenetic relation, and performed transmembrane domain and gene/protein structure analyses. We found that the NRAMP gene family forms two distinct groups. One group included the PvNRAMP1, -6, and -7 genes that share a fragmented structure with a numerous exon/intron organization and encode proteins with mitochondrial or plastidial localization. The other group is characterized by few exons that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Transporter; NRAMP; Gene family; Phylogenetic analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500820

Use of lymphocyte cultures for BrdU replication banding patterns in anuran species (Amphibia)
Provedor de dados: Genetics and Molecular Biology
Autores: Kasahara,Sanae; Silva,Ana Paula Zampieri; Gruber,Simone Lilian.
We describe the standardization of lymphocyte culture procedures in order to improve cytological preparations of anuran species. This methodology permits the use of 5-bromodeoxyuridine (BrdU) treatment to obtain replication banding patterns in the chromosomes of these species.
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400011

Effect of phenobarbital on inducing insecticide tolerance and esterase changes in Aedes aegypti (Diptera: Culicidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Sousa-Polezzi,Rita de Cássia; Bicudo,Hermione Elly Melara de Campos.
The effect of phenobarbital (PB) on the induction of tolerance to the organophosphorous insecticide temephos (TE) was investigated in Aedes aegypti L4 larvae submitted to two different PB-treatments:(1) continuous treatment from the egg to the larval L4 stage and (2) discontinuous treatment in which L4 larvae were exposed for 30 h. Mosquitoes from two Brazilian cities were studied: São José do Rio Preto (SJ) in São Paulo State and Goiânia (GO) in Goiás State. According to criterions established by World Health Organization (WHO) mosquitoes from SJ are organophosphate-susceptible while mosquitoes from GO are organophosphate-resistant. For both SJ and GO larvae the two different PB-treatments resulted in significantly increased tolerance (measured by reduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aedes aegypti; Insecticide resistance; Phenobarbital; Tolerance; Pyperonyl butoxide; Esterases.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200024

JAK2 V617F prevalence in Brazilian patients with polycythemia vera, idiopathic myelofibrosis and essential thrombocythemia
Provedor de dados: Genetics and Molecular Biology
Autores: Monte-Mór,Bárbara da Costa Reis; Cunha,Anderson Ferreira da; Pagnano,Kátia Bórgia Barbosa; Saad,Sara Terezinha; Lorand-Metze,Irene; Costa,Fernando Ferreira.
Polycythemia vera (PV), essential thrombocythemia (ET) and idiopathic myelofibrosis (IMF) are myeloproliferative disorders (MPD) that arise from the clonal proliferation of a pluripotent hematopoietic progenitor, leading to the overproduction of one or more myeloid lineages. Recently, a specific mutation in the JAK2 gene, which encodes a tyrosine kinase, has been shown to be associated with the myeloproliferative phenotype observed in PV, ET and IMF. In this study of Brazilian patients, the JAK2 V617F mutation [c.1887G &gt; T) was detected in four out of 49 patients with PV (96%), 14 out of 25 patients with IMF (56%), and in eight out of 29 patients with ET, which is in accordance with previous screenings of this mutation in other populations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: JAK2 V617F; Myeloproliferative disorders; Polycythemia vera; Idiopathic myelofibrosis; Essential thrombocythemia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300006

Thesis Abstracts
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400033

Sequencing and de novo assembly of the Asian gypsy moth transcriptome using the Illumina platform
Provedor de dados: Genetics and Molecular Biology
Autores: Xiaojun,Fan; Chun,Yang; Jianhong,Liu; Chang,Zhang; Yao,Li.
Abstract The Asian gypsy moth (Lymantria dispar) is a serious pest of forest and shade trees in many Asian and some European countries. However, there have been few studies of L. dispar genetic information and comprehensive genetic analyses of this species are needed in order to understand its genetic and metabolic sensitivities, such as the molting mechanism during larval development. In this study, high-throughput sequencing technology was used to sequence the transcriptome of the Asian subspecies of the gyspy moth, after which a comprehensive analysis of chitin metabolism was undertaken. We generated 37,750,380 high-quality reads and assembled them into contigs. A total of 37,098 unigenes were identified, of which 15,901 were annotated in the NCBI...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asian gypsy moth; Chitin metabolism; Transcriptome; Unigenes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100160

Genetic approaches for studying transgene inheritance and genetic recombination in three successive generations of transformed tobacco
Provedor de dados: Genetics and Molecular Biology
Autores: Tizaoui,Kalthoum; Kchouk,Mohamed Elyes.
Transgene integration into plant genomes is a complex process accompanied by molecular rearrangements. Classic methods that are normally used to study transgenic population genetics are generally inadequate for assessing such integration. Two major characteristics of transgenic populations are that a transgenic genome may harbor many copies of the transgene and that molecular rearrangements can create an unstable transgenic locus. In this work, we examined the segregation of T1, T2 and T3 transgenic tobacco progenies. Since transfer DNA (T-DNA) contains the NptII selectable marker gene that confers resistance to kanamycin, we used this characteristic in developing a method to estimate the number of functional inserts integrated into the genome. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copy number variation; Gene rearrangements; Genetic inheritance; Transgenic.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400015

Genetic polymorphisms of Interleukin-18 are not associated with allograft function in kidney transplant recipients
Provedor de dados: Genetics and Molecular Biology
Autores: Nascimento,Wenna Gleyce Araújo do; Cilião,Daiani Alves; Genre,Julieta; Gondim,Dikson Dibe; Alves,Renata Gomes; Hassan,Neife Deghaide; Lima,Francisco Pignataro; Pereira,Maurício Galvão; Donadi,Eduardo Antônio; Crispim,Janaina Cristiana de Oliveira.
Interleukin 18 (IL-18) is a proinflammatory cytokine that plays a role in host defense by upregulating both innate and acquired immune responses. Analysis of IL 18 polymorphisms may be clinically important since their roles have been recognized in a variety of inflammatory and autoimmune disorders. However, the role of this cytokine polymorphisms in kidney transplant still remains unclear. In this study, we evaluated the associations between IL 18 polymorphisms and graft function assessed by creatinine clearance in kidney transplant recipients. A total of 82 kidney transplant recipients and 183 healthy controls were enrolled, and frequencies of alleles, genotypes and haplotypes for IL 18 polymorphisms were determined and compared with creatinine clearance....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IL-18; -607C/A; -137C/G; Kidney transplant.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300005

Cytogenotoxicity biomarkers in fat snook  Centropomus parallelus  from Cananéia and São Vicente estuaries, SP, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Kirschbaum,Aline A.; Seriani,Robson; Pereira,Camilo D. S.; Assunção,Andrea; Abessa,Denis M. de Souza; Rotundo,Matheus M.; Ranzani-Paiva,Maria J. T.
The aquatic environment receives many contaminants that can induce damages at the molecular, biochemical, cellular and physiological levels.  Centropomus parallelus , an important food resource for local populations, is a predator fish that feeds on small fishes and benthic invertebrates, thus being vulnerable to the bioconcentration and biomagnification processes. This study aimed to evaluate cytogenotoxic responses in erythrocytes from  C. parallelus  juveniles collected in the Cananéia and São Vicente estuaries, both in winter and in summer. After anesthesia, blood samples were collected by caudal puncture. Blood smears were prepared on glass slides and stained with May-Grünwald-Giemsa dye. Two thousand cells were analyzed per slide (1000x), and nuclear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenotoxicity; Nuclear abnormalities; Micronuclei; Centropomus parallelus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100023

Extinction and emergence of genomic haplotypes during the evolution of Avian coronavirus in chicken embryos
Provedor de dados: Genetics and Molecular Biology
Autores: Brandão,Paulo E.; Hora,Aline S.; Silva,Sheila O. S.; Taniwaki,Sueli A.; Berg,Mikael.
Abstract Avian coronavirus (AvCoV) is ubiquitously present on poultry as a multitude of virus lineages. Studies on AvCoV phenotypic traits are dependent on the isolation of field strains in chicken embryonated eggs, but the mutant spectrum on each isolate is not considered. This manuscript reports the previously unknown HTS (high throughput sequencing)-based complete genome haplotyping of AvCoV isolates after passages of two field strains in chicken embryonated eggs. For the first and third passages of strain 23/2013, virus loads were 6.699 log copies/ μL and 6 log copies/ μL and, for 38/2013, 5.699 log copies/μL and 2.699 log copies/μL of reaction, respectively. The first passage of strain 23/2013 contained no variant haplotype, while, for the third...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian coronavirus; Haplotype; Mutant spectrum; NGS; Evolution.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400202

Molecular cloning and sequence analysis of growth hormone cDNA of Neotropical freshwater fish Pacu (Piaractus mesopotamicus)
Provedor de dados: Genetics and Molecular Biology
Autores: Pinheiro,Janeth Silva; Wolff,José Luiz Caldas; Araújo,Ronaldo de Carvalho; Hilsdorf,Alexandre Wagner Silva.
RT-PCR was used for amplifying Piaractus mesopotamicus growth hormone (GH) cDNA obtained from mRNA extracted from pituitary cells. The amplified fragment was cloned and the complete cDNA sequence was determined. The cloned cDNA encompassed a sequence of 543 nucleotides that encoded a polypeptide of 178 amino acids corresponding to mature P. mesopotamicus GH. Comparison with other GH sequences showed a gap of 10 amino acids localized in the N terminus of the putative polypeptide of P. mesopotamicus. This same gap was also observed in other members of the family. Neighbor-joining tree analysis with GH sequences from fishes belonging to different taxonomic groups placed the P. mesopotamicus GH within the Otophysi group. To our knowledge, this is the first GH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piaractus mesopotamicus; Growth hormone; CDNA cloning; Sequencing.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200037

Low cytomolecular diversification in the genus Stylosanthes Sw. (Papilionoideae, Leguminosae)
Provedor de dados: Genetics and Molecular Biology
Autores: Franco,Ana Luiza; Figueredo,Amanda; Pereira,Lívia de Moraes; Sousa,Saulo Marçal de; Souza,Gustavo; Carvalho,Marcelo Ayres; Simon,Marcelo F.; Viccini,Lyderson Facio.
Abstract Stylosanthes (Papilionoideae, Leguminosae) is a predominantly Neotropical genus with ~48 species that include worldwide important forage species. This study presents the chromosome number and morphology of eight species of the genus Stylosanthes (S. acuminata, S. gracilis, S. grandifolia, S. guianensis, S. hippocampoides, S. pilosa, S. macrocephala, and S. ruellioides). In addition, staining with CMA and DAPI, in situ hybridization with 5S and 35S rDNA probes, and estimation of DNA content were performed. The interpretation of Stylosanthes chromosome diversification was anchored by a comparison with the sister genus Arachis and a dated molecular phylogeny based on nuclear and plastid loci. Stylosanthes species showed 2n = 20, with low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachis; Cytogenetics; Evolution; Leguminosae; Stylosanthes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100306

Erratum
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400534

Nucleotide diversity and molecular evolution of the WAG-2 gene in common wheat (Triticum aestivum L) and its relatives
Provedor de dados: Genetics and Molecular Biology
Autores: Wei,Shuhong; Peng,Zhengsong; Zhou,Yonghong; Yang,Zaijun; Wu,Kai; Ouyang,Zhongming.
In this work, we examined the genetic diversity and evolution of the WAG-2 gene based on new WAG-2 alleles isolated from wheat and its relatives. Only single nucleotide polymorphisms (SNP) and no insertions and deletions (indels) were found in exon sequences of WAG-2 from different species. More SNPs and indels occurred in introns than in exons. For exons, exons+introns and introns, the nucleotide polymorphism Π decreased from diploid and tetraploid genotypes to hexaploid genotypes. This finding indicated that the diversity of WAG-2 in diploids was greater than in hexaploids because of the strong selection pressure on the latter. All dn/ds ratios were &lt; 1.0, indicating that WAG-2 belongs to a conserved gene affected by negative selection. Thirty-nine of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copy number variation; Genetic diversity; Molecular evolution; Triticum; WAG-2.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400013

A lack of association between adiponectin polymorphisms and coronary artery disease in a Chinese population
Provedor de dados: Genetics and Molecular Biology
Autores: Zhong,Chen; Zhen,Ding; Qian,Qi; Genshan,Ma.
We investigated the association between two single nucleotide polymorphisms (SNPs) in the adiponectin gene (rs822395 and rs266729) and coronary artery disease (CAD) in a case-control study of 198 unrelated Chinese CAD patients (with ; 70% coronary stenosis or previous myocardial infarction) and 237 non-CAD controls. The ligase reaction was used to detect SNPs rs822395 and rs266729, and the allelic association of these SNPs with the occurrence and severity of CAD was assessed. There were no significant differences in the genotypic or allelic frequencies of the two SNPs between control and CAD individuals. In addition, there was no association between the two SNPs and the severity of CAD based on the number of diseased vessels. The frequencies of alleles C...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adiponectin gene; Coronary artery disease; Single nucleotide polymorphisms.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300005

Restoring pollen fertility in transgenic male-sterile eggplant by Cre/loxp-mediated site-specific recombination system
Provedor de dados: Genetics and Molecular Biology
Autores: Cao,Bihao; Huang,Zhiyin; Chen,Guoju; Lei,Jianjun.
This study was designed to control plant fertility by cell lethal gene Barnase expressing at specific developmental stage and in specific tissue of male organ under the control of Cre/loxP system, for heterosis breeding, producing hybrid seed of eggplant. The Barnase-coding region was flanked by loxP recognition sites for Cre-recombinase. The eggplant inbred/pure line ('E-38') was transformed with Cre gene and the inbred/pure line ('E-8') was transformed with the Barnase gene situated between loxp. The experiments were done separately, by means of Agrobacterium co-culture. Four T0-plants with the Barnase gene were obtained, all proved to be male-sterile and incapable of producing viable pollen. Flowers stamens were shorter, but the vegetative phenotype was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eggplant; Male sterility; Barnase gene; Cre gene; Cre/loxP system.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200017

Sequence polymorphism from EST data in sugarcane: a fine analysis of 6-phosphogluconate dehydrogenase genes
Provedor de dados: Genetics and Molecular Biology
Autores: Grivet,L.; Glaszmann,J.C.; Arruda,P.
This paper presents preliminary results demonstrating the use of the sugarcane expressed sequence tag (EST) database (SUCEST) to detect single nucleotide polymorphisms (SNPs) inside 6-phosphogluconate dehydrogenase genes (Pgds). Sixty-four Pgd-related EST sequences were identified and partitioned into two clear-cut sets of 14 and 50 ESTs, probably corresponding to two genes, A and B, respectively. Alignment of A sequences allowed the detection of a single SNP while alignment of B sequences permitted the detection of 39 reliable SNPs, 27 of which in the coding sequence of the gene. Thirty-eight SNPs were binucleotidic and a single one was trinucleotidic. Nine insertions/deletions from one to 72 base pairs long were also detected in the noncoding 3’ and 5’...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100022

Mitochondrial genomes of genus Atta (Formicidae: Myrmicinae) reveal high gene organization and giant intergenic spacers
Provedor de dados: Genetics and Molecular Biology
Autores: Barbosa,Josefa T. V.; Barbosa,Marcílio S.; Morais,Suzyane; Santana,Antônio E. G.; Almeida,Cicero.
Abstract The ants of the genus Atta are considered important pests to agriculture in the Americas, although Atta species are also important contributors to ecosystem functions in the various habitats in which they occur. The aim of this study was to assemble four complete mitochondrial genomes of the genus Atta, construct the phylogenomic tree, and analyze the gene content, order, and organization. The mitogenomes of A. colombica, A. opaciceps, A. texana, and A. sexdens rubropilosa comprise 18,392, 19,257, 19,709, and 19,748 bp, respectively. The four Atta mitogenomes showed the charactistics typical of those of insects, with 13 protein-coding genes, 22 tRNAs, and 2 rRNAs, with genes displayed in the conventional order. Analysis for intergenic spacer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ants; Evolution; Mitogenomes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500801

The post-transcriptional gene silencing pathway in Eucalyptus
Provedor de dados: Genetics and Molecular Biology
Autores: Sassaki,Flávio Tetsuo; Campos-Pereira,Tiago; Maia,Ivan de Godoy.
Post-transcriptional gene silencing (PTGS) is a conserved surveillance mechanism that identifies and cleaves double-stranded RNA molecules and their cellular cognate transcripts. The RNA silencing response is actually used as a powerful technique (named RNA interference) for potent and specific inhibition of gene expression in several organisms. To identify gene products in Eucalyptus sharing similarities with enzymes involved in the PTGS pathway, we queried the expressed sequence tag database of the Brazilian Eucalyptus Genome Sequence Project Consortium (FORESTs) with the amino acid sequences of known PTGS-related proteins. Among twenty-six prospected genes, our search detected fifteen assembled sequences encoding products presenting high level of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene silencing; PTGS; RNA silencing; EST; Eucalyptus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400003

Identification and description of distinct B chromosomes in Cyphocharax modestus (Characiformes, Curimatidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Lessandra Viviane De Rosa; Foresti,Fausto; Martins,Cesar; Oliveira,Claudio; Wasko,Adriane Pinto.
Cytogenetic analyses were performed in Cyphocharax modestus, collected at Paranapanema River and Tietê River (São Paulo State, Brazil). A karyotype with 2n = 54 chromosomes was observed in the animals from both Brazilian freshwater river systems. One to four B chromosomes were also detected in individuals from the Paranapanema River, which represents the probable first report of more than a single supernumerary element in a species of the Curimatidae group. C-banding revealed centromeric and telomeric heterochromatin blocks in several chromosomes of the normal karyotype complement of C. modestus. Moreover, while some B chromosomes were characterized by the complete absence of C-bands, others were totally heterochromatic. Although there was a prevalence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B chromosomes; C-banding; Curimatidae; Cyphocharax modestus; Supernumerary chromosomes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200019

Molecular chaperone genes in the sugarcane expressed sequence database (SUCEST)
Provedor de dados: Genetics and Molecular Biology
Autores: Borges,Júlio C.; Peroto,Maria C.; Ramos,Carlos H.I.
Some newly synthesized proteins require the assistance of molecular chaperones for their correct folding. Chaperones are also involved in the dissolution of protein aggregates making their study significant for both biotechnology and medicine and the identification of chaperones and stress-related protein sequences in different organisms is an important task. We used bioinformatic tools to investigate the information generated by the Sugarcane Expressed Sequence Tag (SUCEST) genome project in order to identify and annotate molecular chaperones. We considered that the SUCEST sequences belonged to this category of proteins when their E-values were lower than 1.0e-05. Our annotation shows that 4,164 of the 5’ expressed sequence tag (EST) sequences were...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100013

Populational genetic structure and sociogenetic structure of cocoon masses of Digelasinus diversipes (Kirby, 1882) (Hymenoptera: Symphyta: Argidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Boraschi,Daniele; Del Lama,Marco Antonio.
Gene variation and population genetic structure of the Neotropical sawfly Digelasinus diversipes were measured by allozyme analyses using starch gel electrophoresis. Cocoon masses were collected in Eugenia glazioviana (Myrtaceae) stems, in two areas of the "Estação Ecológica Jataí" (Luiz Antônio, SP, Brazil - 21°25' S, 47°50' W), in 2000 and 2001. The average heterozygosity observed in this species (Hobs = 0.094 ± 0.025) was not significantly different from other Symphyta groups; it was, however, significantly higher than in other Hymenoptera populations. No significant levels of inbreeding were found (F IS = 0.062; chi2 = 29.9; p &gt; 0.05), but the population was subdivided (F ST = 0.070; chi2 = 458.9; p &lt; 0.05), suggesting the absence of a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Population genetics; Digelasinus diversipes; Neotropical sawfly; Allozymes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300013

Lack of an association between SNPs within the cholinergic receptor genes and smoking behavior in a Czech post-MONICA study
Provedor de dados: Genetics and Molecular Biology
Autores: Hubacek,Jaroslav A.; Lanska,Vera; Adamkova,Vera.
Smoking has a significant heritable component of approximately 30-60%. Recent genome wide association studies have identified single nucleotide polymorphisms (SNPs) within the nicotinic cholinergic receptor subunits 3 (rs578776), 5 (rs16969968) and β3 (rs6474412), which are associated with nicotine dependence in Western European populations. To analyze the association in a Czech population, we genotyped 1,191 males and 1,368 females (post-MONICA study). The WHO protocol was used to examine smoking status and the number of cigarettes smoked per day. There were 32.1% current and 27.6% past smokers among the males and 22.5% current and 13.8% past smokers among the females. We have not confirmed the original results: the SNPs rs16969968 (p = 0.07), rs578776 (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholinergic receptors; Polymorphism; Smoking.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500004

Chromosomal phylogeny of the Drosophila fasciola species subgroup revisited (Diptera, Drosophilidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz,Nilda Maria; Sene,Fabio Melo.
The analysis of polytene chromosomes in 26 strains of seven species in the Drosophila fasciola subgroup, from several locations in Brazil, in addition to strains of two species belonging to the Drosophila mulleri subgroup (D. aldrichi and D. mulleri), enabled us to determine that the 3c inversion found in the latter species differ in one of its break points from that present in the species of the fasciola subgroup. Therefore, a change in the mulleri complex denomination from inversion 3c to inversion 3u is proposed. Accordingly, the fasciola subgroup is no longer a lesser phylogenetic part within the mulleri subgroup. Rather, it is directly related to the likely ancestor of the repleta group, called Primitive I. This information removes the main obstacle...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila; Chromosome phylogeny; Repleta group; Fasciola subgroup; Chromosome inversions.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400016

Evaluation of the genotoxic potential of the Hypericum brasiliense (Guttiferae) extract in mammalian cell system in vivo
Provedor de dados: Genetics and Molecular Biology
Autores: Espósito,Anderson Victorino; Pereira,Danielli Maria Vieira; Rocha,Leandro Machado; Carvalho,José Carlos Tavares; Maistro,Edson Luis.
Plants of the genus Hypericum, long used in folk medicine, contain active compounds which present, anti-septic, diuretic, digestive, expectorant, vermifugal, anti-depressive and other properties. The possible clastogenic effect of a H. brasiliense extract was tested in vivo on the bone marrow cells of Wistar rats. The extract was administered by gavage at doses of 50, 150 and 300 mg/kg body weight. Experimental and control animals were submitted to euthanasia 24 h after the treatment for micronucleus (MN) and chromosome preparations. H. brasiliense extract did not induce statistically significant increases in the average numbers of MN or chromosome aberrations in the test systems employed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypericum brasiliense; Micronucleus test; Chromosome aberrations.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100026

Combination of meta-analysis and graph clustering to identify prognostic markers of ESCC
Provedor de dados: Genetics and Molecular Biology
Autores: Gao,Hongyun; Wang,Lishan; Cui,Shitao; Wang,Mingsong.
Esophageal squamous cell carcinoma (ESCC) is one of the most malignant gastrointestinal cancers and occurs at a high frequency rate in China and other Asian countries. Recently, several molecular markers were identified for predicting ESCC. Notwithstanding, additional prognostic markers, with a clear understanding of their underlying roles, are still required. Through bioinformatics, a graph-clustering method by DPClus was used to detect co-expressed modules. The aim was to identify a set of discriminating genes that could be used for predicting ESCC through graph-clustering and GO-term analysis. The results showed that CXCL12, CYP2C9, TGM3, MAL, S100A9, EMP-1 and SPRR3 were highly associated with ESCC development. In our study, all their predicted roles...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esophageal squamous cell carcinoma; Meta-analysis; Graph clustering.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300021

Tetrasomy 3q26.32-q29 due to a supernumerary marker chromosome in a child with pigmentary mosaicism of Ito
Provedor de dados: Genetics and Molecular Biology
Autores: Cunha,Karina S.; Simioni,Milena; Vieira,Tarsis P.; Gil-da-Silva-Lopes,Vera L.; Puzzi,Maria B.; Steiner,Carlos E.
Abstract Pigmentary mosaicism of Ito (PMI) is a skin abnormality often characterized by hypopigmentation of skin, following, in most cases, the Blaschko lines, usually associated with extracutaneous abnormalities, especially abnormalities of the central nervous system (CNS). It is suggested that this pattern arises from the presence and migration of two cell lineages in the ectoderm layer during the embryonic period and embryonic cell migration, with different gene expression profiles associated with pigmentation. Several types of chromosomal aberrations, with or without mosaicism, have been associated with this disorder. This study comprised clinical description and cytogenetic analysis of a child with PMI. The G-banded karyotype analysis revealed a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pigmentary mosaicism of Ito; Tetrasomy; Supernumerary marker chromosome; Array genomic hybridization.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100035

Cutis laxa with growth and developmental delay, wrinkly skin syndrome and gerodermia osteodysplastica: a report of two unrelated patients and a literature review
Provedor de dados: Genetics and Molecular Biology
Autores: Steiner,Carlos Eduardo; Cintra,Maria Letícia; Marques-de-Faria,Antonia Paula.
Two unrelated patients of different sexes are described, both presenting with congenital redundant skin (cutis laxa), growth deficiency, mental retardation and bone dystrophy. Parental consanguinity in both families and a more pronounced severity of the neurological disease in the male patient were present. Both patients were diagnosed in infancy as having De Barsy syndrome, but clinical follow-up revealed that the clinical picture was compatible with the diagnosis of cutis laxa with growth and developmental delay (CLGDD), gerodermia osteodysplastica (GO) and wrinkly-skin syndrome (WWS). It has recently been suggested that cutis laxa with growth and developmental delay, gerodermia osteodysplastica and wrinkly skin syndrome are the same condition. A review...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutis laxa; De Barsy syndrome; Gerodermia osteodysplastica; Wrinkly skin syndrome.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200001

Lack of functional KL-VS polymorphism of the KLOTHO gene in the Korean population
Provedor de dados: Genetics and Molecular Biology
Autores: Kim,Hee-Kwon; Jeong,Byung-Hoon.
Abstract The functional variant of the Klotho "KL-VS" stretch, which includes six polymorphisms in linkage disequilibrium, is reportedly associated with healthy aging and longevity in European and American populations. Among Asian populations, this variant has been observed in the Indian population but not in the Iranian population. An association between KL-VS polymorphism and aging has not been reported in Koreans. To investigate whether the KL-VS polymorphism could be associated with healthy aging and longevity in a Korean population, we analyzed genotype and allele frequencies of the KL-VS variant in a large Korean population sample. The KL-VS variant was not found in 874 Korean individuals. Thus, it is not possible to test its association to aging in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Aging; KLOTHO gene; KL-VS polymorphism; Population genetics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300370

Effects of β-glucan polysaccharide revealed by the dominant lethal assay and micronucleus assays, and reproductive performance of male mice exposed to cyclophosphamide
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Rodrigo Juliano; Pesarini,João Renato; Salles,Maria José Sparça; Kanno,Tatiane Yumi Nakamura; Lourenço,Ana Carolina dos Santos; Leite,Véssia da Silva; Silva,Ariane Fernanda da; Matiazi,Hevenilton José; Ribeiro,Lúcia Regina; Mantovani,Mário Sérgio.
β-glucan is a well-known polysaccharide for its chemopreventive effect. This study aimed to evaluate the chemopreventive ability of β-glucan in somatic and germ cells through the dominant lethal and micronucleus assays, and its influence on the reproductive performance of male mice exposed to cyclophosphamide. The results indicate that β-glucan is capable of preventing changes in DNA in both germ cells and somatic ones. Changes in germ cells were evaluated by the dominant lethal assay and showed damage reduction percentages of 46.46% and 43.79% for the doses of 100 and 150 mg/kg. For the somatic changes, evaluated by micronucleus assay in peripheral blood cells in the first week of treatment, damage reduction percentages from 80.63-116.32% were found. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutation; Post-implantation losses; Chemoprevention; Micronucleus; Nulliparous females.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100017

Linkage disequilibrium and past effective population size in native Tunisian cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Jemaa,Slim Ben; Thamri,Nejia; Mnara,Sofiane; Rebours,Emmanuelle; Rocha,Dominique; Boussaha,Mekki.
Abstract To carry out effective genome-wide association studies, information about linkage disequilibrium (LD) is essential. Here, we used medium-density SNP chips to provide estimates of LD in native Tunisian cattle. The two measures of LD that were used, mean r2 and D’, decreased from 0.26 to 0.05 and from 0.73 to 0.40, respectively, when the distance between markers increased from less than 20 Kb to 200 Kb. The decay in LD over physical distance occurred at a faster rate than that reported for European and other indigenous breeds, and reached background levels at less than 500 Kb distance. This is consistent with the absence of strong selective pressure within the Tunisian population and suggests that, in order to be effective, any potential genome-wide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tunisian cattle; Conservation; Linkage disequilibrium; Effective population size; Single nucleotide polymorphism.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100052

Leptin and hypothalamic gene expression in early- and late-maturing Bos indicus Nellore heifers
Provedor de dados: Genetics and Molecular Biology
Autores: Vaiciunas,Aline; Coutinho,Luiz L.; Meirelles,Flávio V.; Pires,Alexandre V.; Silva,Luis Felipe P.
We investigated wether early-maturing or late-maturing Bos indicus Nellore heifers produced more leptin mRNA in adipose tissues and altered expression of hypothalamic genes related to leptin signaling. Six prepubertal and six pubertal heifers aged about 34 months and weighing 280 kg to 300 kg each were selected from a population of 100 Nellore heifers. Real-time PCR was used to quantify the expression of the leptin gene (LEP) in adipose tissues and the long isoform of the leptin receptor gene (Ob-Rb), the NK2 homeobox 1 hypothalamic marker gene NKX2-1, the suppressor of cytokine signaling 3 gene (SOCS-3), the neuropeptide Y genes (NPY) and the NPY G-protein coupled receptor genes NPY-Y1 and NPY-Y4 in the hypothalamus. Heifers attaining puberty earlier...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bos indicus; Gene expression; Hypothalamus; Leptin; Neuropeptide Y.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400010

Determination of βS haplotypes in patients with sickle-cell anemia in the state of Rio Grande do Norte, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Cabral,Cynthia Hatsue Kitayama; Serafim,Édvis Santos Soares; Medeiros,Waleska Rayane Dantas Bezerra de; Fernandes,Thales Allyrio Araújo de Medeiros; Kimura,Elza Miyuki; Costa,Fernando Ferreira; Sonati,Maria de Fátima; Rebecchi,Ivanise Marina Moretti; Medeiros,Tereza Maria Dantas de.
βS haplotypes were studied in 47 non-related patients with sickle-cell anemia from the state of Rio Grande do Norte, Brazil. Molecular analysis was conducted by PCR/RFLP using restriction endonucleases XmnI, HindIII, HincII and HinfI to analyze six polymorphic sites from the beta cluster. Twenty-seven patients (57.5%) were identified with genotype CAR/CAR, 9 (19.1%) CAR/BEN, 6 (12.8%) CAR/CAM, 1 (2.1%) BEN/BEN, 2 (4.3%) CAR/Atp, 1 (2.1%) BEN/Atp and 1 (2.1%) with genotype Atp/Atp. The greater frequency of Cameroon haplotypes compared to other Brazilian states suggests the existence of a peculiarity of African origin in the state of Rio Grande do Norte.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haplotypes; Β-globin; Sickle-cell anemia; Brazilian population; S hemoglobin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300009

Toxicity and genotoxicity of wastewater from gasoline stations
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira-Martins,Cynthia R.; Grisolia,Cesar K.
The toxicity and genotoxicity of wastewater from eight gasoline stations in Brasília, Brazil's capital city, was studied by assessing chromosomal aberrations, chromosomal malsegregation and the mitotic index in Allium cepa root cells, and the occurrence of micronucleus and nuclear abnormalities in peripheral erythrocytes of tilapia fish (Oreochromis niloticus). The content of gasoline station effluents was also analyzed based on several physico-chemical parameters. None of the wastewater samples was genotoxic to A. cepa root cells, although cell proliferation was significantly inhibited, especially at the highest concentrations. Likewise, no micronuclei were observed in O. niloticus peripheral erythrocytes, even after exposure to high concentrations, but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Chromosomal aberrations; Gas station; Micronucleus; Oreochromis niloticus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400029

Prediction of seed-yield potential of common bean populations
Provedor de dados: Genetics and Molecular Biology
Autores: Abreu,Ângela de Fátima Barbosa; Ramalho,Magno Antonio Patto; Santos,João Bosco dos.
Earliest possible prediction of seed-yield potential of autogamous crop populations increases breeding program efficiency by saving time and resources. Alternatives for obtaining seed-yield predictions were compared by evaluating four common-bean populations in F1 and F2 generations together with the parents. Mean components (m + a' and d) and variances were estimated. The potential of each population was predicted by using both these and the Jinks and Pooni (1976) procedure, which allows probability estimation of each population of originating lines surpassing a determined standard. Estimate efficiency was determined by evaluating performances of 62 F5:7 families from each population. Mean component m + a' estimates obtained for the F1 and F2 generations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Choice of parents; Heritability; Mean components; Common bean; Phaseolus vulgaris L.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300013

Interaction between ABO and haptoglobin systems in a Chilean population of  mixed ancestry
Provedor de dados: Genetics and Molecular Biology
Autores: Acuña,M.; Eaton,L.
In order to investigate the association between ABO blood groups and serum haptoglobin (Hp), we typed Hp and ABO blood groups in 288 children, who were selected at random among those who began their primary school in 1973 in the Northern Metropolitan area of Santiago, Chile. This region has an Amerindian component of 40% and a Caucasian component (principally Spanish) of 60%. Our results are in contrast with previous studies which have reported that Hp*1 varied among ABO groups in the following manner, O &amp;lt; A &amp;lt; B &amp;lt; AB, and where Hp*1 was significantly lower in group O individuals. In the present study, the order was A &amp;lt; O » B &amp;lt; AB. We found more A and less O individuals with allele Hp*2 and genotype Hp 2-2, and more Hp*1...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400004

The complete mitochondrial genome of Engyodontium album and comparative analyses with Ascomycota mitogenomes
Provedor de dados: Genetics and Molecular Biology
Autores: Yuan,Xiao-Long; Mao,Xin-Xin; Liu,Xin-Min; Cheng,Sen; Zhang,Peng; Zhang,Zhong-Feng.
Abstract Engyodontium album is a widespread pathogen that causes different kinds of dermatoses and respiratory tract diseases in humans and animals. In spite of its perniciousness, the basic genetic and molecular background of this species remains poorly understood. In this study, the mitochondrial genome sequence of E. album was determined using a high-throughput sequencing platform. The circular mitogenome was found to be 28,081 nucleotides in length and comprised of 17 protein-coding genes, 24 tRNA genes, and 2 rRNA genes. The nucleotide composition of the genome was A+T-biased (74.13%). Group-II introns were found in the nad1, nad5, and cob genes. The most frequently used codon of protein-coding genes was UAU. Isoleucine was identified as the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Engyodontium album; Mitochondrial genome; Comparative analysis; Phylogenetic analyses.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500844

Combining ability of Phaseolus vulgaris L. for resistance to common bacterial blight
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Rosana; Leal,Nilton Rocha; Pereira,Messias Gonzaga; Lam-Sánchez,Alfredo.
Many diseases limit dry bean and snap bean yields. Common bacterial blight (CBB), caused by Xanthomonas axonopodis pv. phaseoli, is one of the most serious bacterial diseases in dry bean and snap bean that cause crop losses. Since there is no satisfactory chemical control, the use of resistant cultivars is an important control measure. Genetic studies of resistance are important for choosing appropriate breeding methods. Combining ability was determined for disease resistance in three snap bean genotypes (Alessa, Hab 52 and Hab 198) and two dry bean genotypes (Bac-6 and A-794). Plants were inoculated with highly pathogenic isolate CNF 15, using a razor blade procedure in leaves and needle punctures in pods. They were evaluated 7 days after inoculation....
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400018

Contribution to the validation of the anaphase-telophase test: aneugenic and clastogenic effects of cadmium sulfate, potassium dichromate and nickel chloride in Chinese hamster ovary cells
Provedor de dados: Genetics and Molecular Biology
Autores: Seoane,A.I.; Dulout,F.N.
There is increasing evidence that aneuploidy during mitosis may be a factor in the etiology of somatic malignancy. The analysis of alterations in anaphase-telophase of mitosis is a useful test for evaluating the aneuploidogenic and clastogenic ability of chemicals. Several metals have been found to be carcinogenic to humans and animals. However, the underlying mechanisms remain unclear. In the present study the aneugenic and clastogenic abilities of cadmium sulfate, potassium dichromate and nickel chloride were analyzed using the anaphase-telophase test. Chinese hamster ovary (CHO) cells cultured for two cycles were treated with the desired compound for 8 h before cell harvesting. The frequency of cells with chromatin bridges, lagging chromosomes and...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400015

Predicting the progress of colon cancer by DNA methylation markers of the p16 gene in feces - Evidence from an animal model
Provedor de dados: Genetics and Molecular Biology
Autores: Wu,Wen-Chih; Hsu,Chih-Hsiung; Kuan,Jen-Chun; Hsieh,Jih-Fu; Sun,Chien-An; Yang,Tsan; Wu,Chang-Chieh; Chou,Yu-Ching.
A new noninvasive screening tool for colorectal neoplasia detects epigenetic alterations exhibited by gastrointestinal tumor cells shed into stool. There is insufficient existing data to determine temporal associations between colorectal cancer (CRC) progression and aberrant DNA methylation. To evaluate the feasibility of using fecal DNA methylation status to determine CRC progression, we collected stool samples from 14 male SD rats aged six weeks, and administered subcutaneous injections of either 1,2-dimethylhydrazine or saline weekly. p16 DNA methylation statuses in tumorous and normal colon tissue, and from stool samples were determined using methylation-specific PCR. Additionally, p16 methylation was detected in stool DNA from 85.7% of the CRC rats....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colorectal cancer; DNA methylation; Stool test for colorectal cancer.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300004

Mantel test in population genetics
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz-Filho,José Alexandre F.; Soares,Thannya N.; Lima,Jacqueline S.; Dobrovolski,Ricardo; Landeiro,Victor Lemes; Telles,Mariana Pires de Campos; Rangel,Thiago F.; Bini,Luis Mauricio.
The comparison of genetic divergence or genetic distances, estimated by pairwise F ST and related statistics, with geographical distances by Mantel test is one of the most popular approaches to evaluate spatial processes driving population structure. There have been, however, recent criticisms and discussions on the statistical performance of the Mantel test. Simultaneously, alternative frameworks for data analyses are being proposed. Here, we review the Mantel test and its variations, including Mantel correlograms and partial correlations and regressions. For illustrative purposes, we studied spatial genetic divergence among 25 populations of Dipteryx alata ("Baru"), a tree species endemic to the Cerrado, the Brazilian savannas, based on 8 microsatellite...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: "Baru" tree; Genetic distances; Geographical genetics; Partial correlation; Partial regression.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400002

Antirecombinagenic activity of propolis against recombinagenic activity of doxorubicin in Drosophila melanogaster
Provedor de dados: Genetics and Molecular Biology
Autores: Valadares,Bruno Lassmar Bueno.
Tipo: Info:eu-repo/semantics/other
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300018

Fritz Müller – Darwin's scientific correspondent in Brazil for 17 years
Provedor de dados: Genetics and Molecular Biology
Autores: Hartfelder,Klaus.
Tipo: Info:eu-repo/semantics/review
Palavras-chave: Darwinian theory; Für Darwin; Evolutionary Developmental Biology; Crustcean phylogeny; Müllerian mimicry.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400722

Genealogical data in population medical genetics: field guidelines
Provedor de dados: Genetics and Molecular Biology
Autores: Poletta,Fernando A.; Orioli,Ieda M.; Castilla,Eduardo E.
This is a guide for fieldwork in Population Medical Genetics research projects. Data collection, handling, and analysis from large pedigrees require the use of specific tools and methods not widely familiar to human geneticists, unfortunately leading to ineffective graphic pedigrees. Initially, the objective of the pedigree must be decided, and the available information sources need to be identified and validated. Data collection and recording by the tabulated method is advocated, and the involved techniques are presented. Genealogical and personal information are the two main components of pedigree data. While the latter is unique to each investigation project, the former is solely represented by gametic links between persons. The triad of a given...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medical genetics; Population medical genetics; Geographic clusters; Isolates; Rare diseases.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200004

Expression of the CTCF gene in bovine oocytes and preimplantation embryos
Provedor de dados: Genetics and Molecular Biology
Autores: Rios,Álvaro F.L.; Lemos,Daniela C.; Fernandes,Maurício B.; Andrea,Maria V.; Gomes,Marcus V.M.; Lôbo,Raysildo B.; Mazucato,Mendelson; Ramos,Ester S.
The CCCTC - binding factor (CTCF) is a protein involved in repression, activation, hormone-inducible gene silencing, functional reading of imprinted genes and X-chromosome inactivation. We analyzed CTCF gene expression in bovine peripheral blood, oocytes and in different cellular stages (2-4 cells, 8-16 cells, 16-32 cells, morulae, and blastocysts) of in vitro fertilized embryos. This is the first report of CTCF expression in oocytes and preimplantation bovine embryos and has implications for the production of embryonic stem cells and the development of novel medical technologies for humans.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CTCF; Genomic imprinting; Preimplantation embryos; X-inactivation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600029

Multiple soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Aquino-Silva,Maria Regina de; Schwantes,Maria Luiza B.; Schwantes,Arno Rudi.
A recent locus duplication hypothesis for sMDH-B* was proposed to explain the complex electrophoretic pattern of six bands detected for the soluble form of malate dehydrogenase (MDH, EC 1.1.1.37) in 84% of the Geophagus brasiliensis (Cichlidae, Perciformes) analyzed (AB1B2 individuals). Klebe's serial dilutions were carried out in skeletal muscle extracts. B1 and B2 subunits had the same visual end-points, reflecting a nondivergent pattern for these B-duplicated genes. Since there is no evidence of polyploidy in the Cichlidae family, MDH-B* loci must have evolved from regional gene duplication. Tissue specificities, thermostability and kinetic tests resulted in similar responses from both B-isoforms, in both sMDH phenotypes, suggesting that these more...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400016

Chromosome numbers for Anthurium and Philodendron spp. (Araceae) occurring in Bahia, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Cotias-de-Oliveira,Ana Lúcia Pires; Guedes,Maria Lenise Silva; Barreto,Ervene Cerqueira.
Chromosome numbers for four species of Anthurium and four species of Philodendron from Bahia, Brazil, were determined. New counts 2n = 30 for A. longipes and A. affine, 2n = 32 for P. pedatum and 2n = 34 P. blanchetianum and P. pachyphyllum represent the first reports for these species. The 2n = 32 found for P. imbe and 2n = 90 for A. bellum differ from earlier reports, whereas 2n = 30 and 60 for A. pentaphyllum var. pentaphyllum confirms previous counts. A. affine had one to four B-chromosomes. We suggest secondary base numbers x = 15 for Anthurium and x = 16, 17 and 18 for Philodendron, produced by hybridizations and duplications involving the primary base numbers x = 7, 8 and 9.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200018

Satellite DNA sites in four species of the genus Astyanax (Teleostei, Characiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Kavalco,Karine Frehner; Pazza,Rubens; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando.
Cytogenetic data about satellite DNA distribution in four Astyanax species (Characidae) from the Paraitinga river, Paraíba do Sul river basin, Brazil, are presented. In order to characterize the constitutive heterochromatin, C-banding, chromomycin A3 and DAPI fluorescence staining, as well as fluorescence in situ hybridization (FISH) with the satellite DNA As-51 probe were performed. A. scabripinnis and A. parahybae presented 2n = 50 and 2n = 48 chromosomes, respectively. The heterochromatin was located in the pericentromeric and terminal regions of many chromosomes, corresponding to GC-positive regions and to the As-51 satellite DNA in terminal regions. A. intermedius and A. giton, both with 2n = 50 chromosomes, showed little heterochromatin, mostly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astyanax; Fluorescence in situ hybridization (FISH); Heterochromatin; Karyotypic diversity; Satellite DNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400005

Mapping of QTL for total spikelet number per spike on chromosome 2D in wheat using a high-density genetic map
Provedor de dados: Genetics and Molecular Biology
Autores: Deng,Mei; Wu,Fangkun; Zhou,Wanlin; Li,Jing; Shi,Haoran; Wang,Zhiqiang; Lin,Yu; Yang,Xilan; Wei,Yuming; Zheng,Youliang; Liu,Yaxi.
Abstract Total spikelet number per spike (TSS) is one of the key components of grain yield in wheat. Chromosome (chr.) 2D contains numerous genes that control TSS. In this study, we evaluated 138 F8 recombinant inbred lines (RILs) derived from an F2 population of a synthetic hexaploid wheat line (SHW-L1) and a common wheat cultivar (Chuanmai 32) for TSS in six different environments. To identify quantitative trait loci (QTL) for TSS, we constructed an integrated high-density genetic map of chr. 2D containing two simple sequence repeats, 35 diversity array technology markers, and 143 single nucleotide polymorphisms. We identified three stable QTL for TSS that individually explained 9.7–19.2% of the phenotypic variation and predicted 23 putative candidate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recombinant inbred line; Synthetic hexaploid wheat; Quantitative trait locus; Total spikelet number per spike.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400603

Genetics of anthracnose panel canker disease resistance and its relationship with yield and growth characters in half-sib progenies of rubber tree (Hevea brasiliensis)
Provedor de dados: Genetics and Molecular Biology
Autores: Gonçalves,Paulo de Souza; Furtado,Edson Luiz; Bataglia,Ondino Cleante; Ortolani,Altino Aldo; May,André; Belletti,Giselle Olmos.
Rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell-Arg] anthracnose panel canker disease resistance, caused by Colletotrichum gloeosporioides (Penz.) Sacc., and growth and yield characters were assessed at three years old in the nursery, in 18 half-sib progenies. There were highly significant (P &amp;lt; 0.01) genetic differences among progenies for most characters. The genetic component of variance accounted for 3.37, 6.07, 12.74, 12.13, 19.82 and 3.58% of the phenotypic variance for anthracnose-infected area, anthracnose lesion stripe length, yield, girth, virgin bark thickness and total number of latex vessel rings, respectively. Narrow-sense heritability estimates for the above characters were 13.45, 24.30, 50.97, 48.52, 79.30 and 14.30%,...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400020

Molecular characterization of SSS139, a new satellite DNA family in sibling species of the Drosophila buzzatii cluster
Provedor de dados: Genetics and Molecular Biology
Autores: Franco,Fernando F.; Sene,Fabio M.; Manfrin,Maura H.
We characterized sequences of a novel SSS139 RsaI satellite DNA family in Drosophila gouveai and Drosophila seriema, two members of the Drosophila buzzatii cluster (D. repleta group). The sequences were AT-rich (69%) with a monomer unit length of about 139 bp and contained two direct subrepeats of 14 bp and 16 bp, suggesting that it might have originated by the duplication of smaller sequences. Southern and dot-blot hybridization analyses also detected SSS139 in other Drosophila buzzatii cluster species (D. koepferae, D. antonietae, D. borborema and D. serido) but not in D. buzzatii. These results agree with the marginal phylogenetic position of D. buzzatii within the D. buzzatii cluster.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Drosophila buzzatii cluster; Molecular evolution; Repetitive DNA; Satellite DNA; SSS139 satellite DNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100026

Genetic variability in mitochondrial and nuclear genes of Larus dominicanus (Charadriiformes, Laridae) from the Brazilian coast
Provedor de dados: Genetics and Molecular Biology
Autores: Dantas,Gisele Pires de Mendonça; Meyer,Diogo; Godinho,Raquel; Ferrand,Nuno; Morgante,João Stenghel.
Several phylogeographic studies of seabirds have documented low genetic diversity that has been attributed to bottleneck events or individual capacity for dispersal. Few studies have been done in seabirds on the Brazilian coast and all have shown low genetic differentiation on a wide geographic scale. The Kelp Gull is a common species with a wide distribution in the Southern Hemisphere. In this study, we used mitochondrial and nuclear markers to examine the genetic variability of Kelp Gull populations on the Brazilian coast and compared this variability with that of sub-Antarctic island populations of this species. Kelp Gulls showed extremely low genetic variability for mitochondrial markers (cytb and ATPase) and high diversity for a nuclear locus (intron...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytb; FIB7 intron; Phylogeography; Recent origin; Selective sweep.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500023

Chromosome mapping of 5S rRNA genes differentiates Brazilian populations of Leporellus vittatus (Anostomidae, Characiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Aguilar,Cecilia Teixeira de; Galetti Junior,Pedro Manoel.
Among the anostomid fishes, the genus Leporellus is represented by only three species: L. nattereri, endemic of the Amazon River, L. retropinnis, endemic of the Piracicaba River, and L. vittatus, widely distributed in rivers from Peru, Colombia, Guianas, and different major hydrographic basins of Brazil. A cytogenetic study carried out on specimens of Leporellus vittatus from three major Brazilian hydrographic basins evidenced a karyotype of 54 metacentric and submetacentric chromosomes. C-banding analysis revealed the presence of large pericentromeric heterochromatic segments in all chromosomes and a telomeric block coincident with the NOR sites. Ag, CMA3 or MM staining, and FISH with ribosomal probes located the 45S ribosomal genes on the terminal region...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; C-bands; 45S rDNA; 5S rDNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200004

Mating system of a population of Myracrodruon urundeuva F.F. &amp;amp; M.F. Allemão using the fAFLP molecular marker
Provedor de dados: Genetics and Molecular Biology
Autores: Freitas,Miguel L.M.; Sebbenn,Alexandre M.; Moraes,Mario L.T.; Lemos,Eliana G.M.
The mating system and genetic diversity were studied in a natural population of Myracrodruon urundeuva originating from 30 open-pollinated trees at the Paulo de Faria Ecological Station, SP, Brazil. The progenies were planted on the Teaching and Research Farm of the Ilha Solteira Engineering School, UNESP. Using the fAFLP molecular marker, eleven loci were selected to study the mating system. The mating system was analyzed using the multilocus mixed-mating model. The estimates of genetic divergence between pollen and ovule allele frequencies were significant for eight loci, suggesting nonrandom outcrossing. The estimates of the multilocus outcrossing rate revealed that M. urundeuva possesses a mating system with a predominance of outcrossing events (theta...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myracrodruon urundeuva; Mating system; FAFLP; Molecular marker; Correlation of paternity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300019

Sequence analysis of the rDNA intergenic spacer of Metarhizium strains isolated in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Yanaka-Schäfer,Fabiana Y.; Dall'Onder,Leonara P.; Panichi,Mariana C.; Mendes,Roberta G.; Fagundes,Nelson J.R.; Bandinelli,Josiane B.; Bogo,Maurício R.
To assess the extent of genetic variability of rDNA intergenic spacer (IGS) in Metarhizium sp., 34 strains (27 isolated in Brazil) were sequenced and analyzed together with an additional 20 Metarhizium anisopliae var. anisopliae sequences retrieved from GenBank. Overall, the global nucleotide diversity for the region under study was of 0.090, while for the Brazilian isolates it was only 0.016. Phylogenetic analyses showed four well-supported groups (A, B, C, and D), one of which (D) has not been previously identified. All but one of the Brazilian strains cluster in this novel D phylogroup, suggesting that the genetic variation found in Brazil is a subset of the worldwide M. anisopiliae var. anisopliae variation.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Intergenic spacer; Metarhizium; RDNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100020

Intra- and interspecific chromosome polymorphisms in cultivated Cichorium L. species (Asteraceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Bernardes,Ebenézer C.S.; Benko-Iseppon,Ana M.; Vasconcelos,Santelmo; Carvalho,Reginaldo; Brasileiro-Vidal,Ana C.
Endive (Cichorium endivia L.) and chicory (C. intybus L.) both have 2n = 18, but until now, there has been no detailed karyomorphological characterization. The present work evaluated five accessions of each species using FISH with rDNA probes and fluorochrome staining with CMA and DAPI. Both species presented distinct banding patterns after fluorochrome staining: while endive had proximal CMA++/DAPI- bands in the short arms of pairs 1, 2 and 3, chicory had proximal CMA-positive bands in chromosomes 1 and 3 and interstitial in the short arm of chromosome 8. Among endive accessions, FISH procedures revealed conserved position and number of 5S and 45S rDNA sites (two and three pairs, respectively), associated with the CMA-positive bands. Notwithstanding,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Compositae; Cichorieae; CMA/DAPI; RDNA sites; Chromosome rearrangements.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300010

Artificial family selection based on growth rate in cultivated lines of Litopenaeus vannamei (Decapoda, Penaeidae) from Venezuela
Provedor de dados: Genetics and Molecular Biology
Autores: De Donato,Marcos; Ramirez,Raúl; Howell,Chris; Verginelli,Roberto; Orta,Tomás; Cabrera,Saúl; Mata,Enrique; Manrique,Ramón.
We investigated the effects of family selection on the growth rates of four genetic lines of Litopenaeus vannamei, the Pacific white shrimp, from Venezuela. The mean family weight at selection was 16.79 g and the mean growth rate was 0.169 g d-1 at a mean age of 138 days. The mean growth rates per generation were 0.141 g d-1 for the parental generation (P0), 0.173 g d-1 for the F1 and 0.191 g d-1 for the F2. Survival varied from 83.3% to 94.4%. There were statistically significant differences between lines, with lines B and C, growing at higher temperatures and lower salinities, showing the fastest growth. Regression analysis between growth rate and age for each genetic line showed high r² values, but smaller than those shown between growth rate and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Family selection; Genetic improvement; Growth rate; Litopenaeus vannamei.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500009

Differentiation of Melipona quadrifasciata L. (Hymenoptera, Apidae, Meliponini) subspecies using cytochrome b PCR-RFLP patterns
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Rogério O.; Moretto,Geraldo; Arias,Maria C.; Del Lama,Marco A.
Melipona quadrifasciata quadrifasciata and M. quadrifasciata anthidioides are subspecies of M. quadrifasciata, a stingless bee species common in coastal Brazil. These subspecies are discriminated by the yellow stripe pattern of the abdominal tergites. We found Vsp I restriction patterns in the cytochrome b region closely associated to each subspecies in 155 M. quadrifasciata colonies of different geographical origin. This mitochondrial DNA molecular marker facilitates diagnosis of M. quadrifasciata subspecies matrilines and can be used to establish their natural distribution and identify hybrid colonies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Subspecies differentiation; Melipona quadrifasciata; Cytochrome b; MtDNA; PCR-RFLP patterns.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300009

Comparative karyotypic analysis in the Alstroemeria hookeri Lodd. (Alstroemeriaceae) complex sensu Bayer (1987)
Provedor de dados: Genetics and Molecular Biology
Autores: Baeza,Carlos; Ruiz,Eduardo; Negritto,María.
Alstroemeria L. (Alstroemeriaceae) is an American genus of monocots with two principal distribution centers in Chile and Brazil. In Chile, it is represented by about 32 species, most of them in central Chile, an area known for its high level of endemism. The "complex" Alstroemeria hookeri is endemic to Chile, where it is distributed from the Coquimbo to the Bío-Bío Region. We analyzed the karyotypes of 36 populations of this complex along its natural distribution. Ten metaphases per population were used for chromosome measurements. All analyzed subspecies presented a well defined asymmetric karyotype. The populations of A. hookeri subsp. hookeri collected in the coastal range of the Bío-Bío Region and the populations from the Central Valley of this Region...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alstroemeria hookeri; Complex; Karyotype; Polymorphisms; Chile.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100020

Amplification of a GC-rich heterochromatin in the freshwater fish Leporinus desmotes (Characiformes, Anostomidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Margarido,Vladimir Pavan; Galetti Junior,Pedro Manoel.
This is the first description of the karyotype of Leporinus desmotes. The diploid female number was 2n = 54 meta- and submetacentric chromosomes. The nucleolar organizing regions (NORs) were studied by silver nitrate staining and rDNA fluorescence in situ hybridization (FISH) and were found to be located in the telomeric region of the long arm of the 9th pair. C-banding revealed centromeric and telomeric heterochromatin segments in most chromosomes. Intercalar blocks of heterochromatin were observed in the long arm of six chromosome pairs. Besides a NOR-adjacent heterochromatin, all of the intercalar heterochromatic segments were brightly fluorescent by mithramycin staining. These data suggest that a unique amplification of a primordial GC-rich...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300012

Genetic structure of the snakehead murrel, Channa striata (channidae) based on the cytochrome c oxidase subunit I gene: influence of historical and geomorphological factors
Provedor de dados: Genetics and Molecular Biology
Autores: Jamaluddin,Jamsari Amirul Firdaus; Pau,Tan Min; Siti-Azizah,Mohd Nor.
Nucleotide sequences of a partial cytochrome c oxidase subunit I gene were used to assess the manner in which historical processes and geomorphological effects may have influenced genetic structuring and phylogeographic patterns in Channa striata. Assaying was based on individuals from twelve populations in four river systems, which were separated into two regions, the eastern and western, of the biodiversely rich state of Perak in central Peninsular Malaysia. In 238 specimens, a total of 368-bp sequences with ten polymorphic sites and eleven unique haplotypes were detected. Data on all the twelve populations revealed incomplete divergence due to past historical coalescence and the short period of separation. Nevertheless, SAMOVA and F ST revealed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Channa striata; MtDNA COI; Phylogeography; Population structure.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100026

Protective effects of acerola juice on genotoxicity induced by iron in vivo
Provedor de dados: Genetics and Molecular Biology
Autores: Horta,Roberta Nunes; Kahl,Vivian Francilia Silva; Sarmento,Merielen da Silva; Nunes,Marisa Fernanda Silva; Porto,Carem Rejane Maglione; Andrade,Vanessa Moraes de; Ferraz,Alexandre de Barros Falcão; Silva,Juliana Da.
Abstract Metal ions such as iron can induce DNA damage by inducing reactive oxygen species (ROS) and oxidative stress. Vitamin C is one of the most widely consumed antioxidants worldwide, present in many fruits and vegetables, especially inMalpighia glabra L., popularly known as acerola, native to Brazil. Acerola is considered a functional fruit due to its high antioxidant properties and phenolic contents, and therefore is consumed to prevent diseases or as adjuvant in treatment strategies. Here, the influence of ripe and unripe acerola juices on iron genotoxicity was analyzed in vivo using the comet assay and micronucleus test. The comet assay results showed that acerola juice exerted no genotoxic or antigenotoxic activity. Neither ripe nor unripe acerola...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Comet assay; Micronucleus; Acerola juice; Iron.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100122

Transcriptome analyses of Ditylenchus destructor in responses to cold and desiccation stress
Provedor de dados: Genetics and Molecular Biology
Autores: Ma,Juan; Gao,Bo; Wang,Rongyan; Li,Xiuhua; Chen,Shulong.
Abstract The objective of this study was to identify molecular responses in Ditylenchus destructor to cold and desiccation by means of transcriptomes analyses. A total of 102,517 unigenes were obtained, with an average length of 1,076 bp, in which 58,453 (57%) had a functional annotation. A total of 1154 simple sequence repeats (SSRs) distributed over 1078 unigenes were detected. Gene expression profiles in response to cold and desiccation stress and the expression of specific stress-related genes were compared. Gene ontology analysis and pathway-based analysis were used to further investigate the functions of the differentially expressed genes. The reliability of the sequencing data was verified through quantitative real-time PCR analysis of 19...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potato rot nematode; Transcriptome; Tolerance; Differentially expressed genes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100202

Female competition in mate choice decreases fitness in flies (Drosophila pseudoobscura) from Colombia
Provedor de dados: Genetics and Molecular Biology
Autores: Hoenigsberg,H.F.
Tipo: Info:eu-repo/semantics/other
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200013

Karyotype description of five species of Trichomycterus (Teleostei: Siluriformes: Trichomycteridae)
Provedor de dados: Genetics and Molecular Biology
Autores: Sato,Luciana Ramos; Oliveira,Claudio; Foresti,Fausto.
Trichomycteridae is a family of small catfish which are widely distributed throughout Southern Central America and South America. The present study showed that the cis-Andean species Trichomycterus florensis, Trichomycterus sp. aff. Trichomycterus itatiyae, Trichomycterus reinhardti, Trichomycterus davisi and Trichomycterus auroguttatus had 2n = 54 chromosomes (42 metacentric, 10 submetacentric and 2 subtelocentric), with T. reinhardti, T. auroguttatus and T. sp. aff. T. itatiyae exhibiting only one chromosome pair with silver-stained nucleolus organizer regions (NORs). The cytogenetic data suggest the existence of at least two groups of species in the cis-Andean representatives of the genus Trichomycterus. In the first group the first metacentric pair is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome evolution; NOR banding; Fish.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100008

Heterochromatin differentiation in holocentric chromosomes of Rhynchospora (Cyperaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Vanzela,André L.L.; Guerra,Marcelo.
Holocentric chromosomes of six species of Rhynchospora, R. ciliata, R. pubera, R. riparia and R. barbata (2n = 10), R. nervosa (2n = 30) and R. globosa (2n = 36), were stained with CMA3/DAPI fluorochromes or treated with C-banding and sequentially stained with Giemsa or CMA3/DAPI. Variability in banding pattern was found among the species studied. Heterochromatin was observed on terminal and interstitial chromosome regions, indicating that the holocentric chromosomes of Rhynchospora show a heterochromatin distribution pattern similar to those plant monocentric chromosomes.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200034

Erratum
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300020

Performance and parameterization of the algorithm Simplified Generalized Simulated Annealing
Provedor de dados: Genetics and Molecular Biology
Autores: Dall'Igna Júnior,Alcino; Silva,Renato S.; Mundim,Kleber C.; Dardenne,Laurent E.
The main goal of this study is to find the most effective set of parameters for the Simplified Generalized Simulated Annealing algorithm, SGSA, when applied to distinct cost function as well as to find a possible correlation between the values of these parameters sets and some topological characteristics of the hypersurface of the respective cost function. The SGSA algorithm is an extended and simplified derivative of the GSA algorithm, a Markovian stochastic process based on Tsallis statistics that has been used in many classes of problems, in particular, in biological molecular systems optimization. In all but one of the studied cost functions, the global minimum was found in 100% of the 50 runs. For these functions the best visiting parameter, qV,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Optimization; Generalized simulated annealing.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400024

Effects of gene therapy on cardiovascular symptoms of lysosomal storage diseases
Provedor de dados: Genetics and Molecular Biology
Autores: Poletto,Edina; Pasqualim,Gabriela; Giugliani,Roberto; Matte,Ursula; Baldo,Guilherme.
Abstract Lysosomal storage diseases (LSDs) are inherited conditions caused by impaired lysosomal function and consequent substrate storage, leading to a range of clinical manifestations, including cardiovascular disease. This may lead to significant symptoms and even cardiac failure, which is an important cause of death among patients. Currently available treatments do not completely correct cardiac involvement in the LSDs. Gene therapy has been tested as a therapeutic alternative with promising results for the heart disease. In this review, we present the results of different approaches of gene therapy for LSDs, mainly in animal models, and its effects in the heart, focusing on protocols with cardiac functional analysis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lysosomal storage disease; Gene therapy; Cardiovascular disease; Animal models; Heart.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200261

The transcription factor Snf1p is involved in a Tup1p-independent manner in the glucose regulation of the major methanol metabolism genes of Hansenula polymorpha
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Marcos A.; Genu,Victor; Salmazo,Anita P.T.; Carraro,Dirce M.; Pereira,Gonçalo A.G.
Hansenula polymorpha is a methylotrophic yeast widely employed in biotechnology as a ''protein factory''. Most promoters used for heterologous protein expression, like MOX (methanol oxidase) and DAS (di-hydroxy acetone synthase), are involved in the peroxisomal methanol metabolism (C1 metabolism) and are under strong glucose repression. Interestingly, the MOX promoter is subjected to glucose regulation also in Saccharomyces cerevisiae, a non-methylotrophic yeast in which this phenomenon is well studied. In this species, the transcription factor Tup1p plays an essential role in glucose repression of several genes. This effect is counteracted by the activator Snf1p when glucose is exhausted from medium. Therefore, to test whether this regulatory circuit has...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TUP1; SNF1; Hansenula polymorpha; Glucose repression; MOX.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400017

Genetic evaluation of the mating system in the blue-and-yellow macaw (Ara ararauna, Aves, Psittacidae) by DNA fingerprinting
Provedor de dados: Genetics and Molecular Biology
Autores: Caparroz,Renato; Miyaki,Cristina Y.; Baker,Allan J.
More than 90% of birds are socially monogamous, although genetic studies indicate that many are often not sexually monogamous. In the present study, DNA fingerprinting was used to estimate the genetic relationships between nestlings belonging to the same broods to evaluate the mating system in the socially monogamous macaw, Ara ararauna. We found that in 10 of 11 broods investigated, the nestlings showed genetic similarity levels congruent with values expected among full-sibs, suggesting that they shared the same parents. However, in one brood, the low genetic similarity observed between nestlings could be a result of intraspecific brood parasitism, intraspecific nest competition or extra-pair paternity. These results, along with available behavioral and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ara ararauna; Breeding behavior; DNA fingerprinting; Monogamy; Psittacidae.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100027

Characterization of Brazilian accessions of wild Arachis species of section Arachis (Fabaceae) using heterochromatin detection and fluorescence in situ hybridization (FISH)
Provedor de dados: Genetics and Molecular Biology
Autores: Custódio,Adriana Regina; Seijo,Guillermo; Valls,José Francisco Montenegro.
The cytogenetic characterization of Arachis species is useful for assessing the genomes present in this genus, for establishing the relationship among their representatives and for understanding the variability in the available germplasm. In this study, we used fluorescence in situ hybridization (FISH) to examine the distribution patterns of heterochromatin and rDNA genes in 12 Brazilian accessions of five species of the taxonomic section Arachis. The heterochromatic pattern varied considerably among the species: complements with centromeric bands in all of the chromosomes (A. hoehnei) and complements completely devoid of heterochromatin (A. gregoryi, A. magna) were observed. The number of 45S rDNA loci ranged from two (A. gregoryi) to eight (A....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crop wild relatives; Groundnut; RDNA loci; Brazil.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300011

Genetic parameters for egg and related characteristics of white Leghorn hens in a subtropical environment
Provedor de dados: Genetics and Molecular Biology
Autores: Sabri,Hani M.; Wilson,Henry R.; Harms,Robert H.; Wilcox,Charles J.
Estimates of heritability and phenotypic and genetic correlations between egg number, weight, specific gravity, mass, and estimated shell weight were obtained, along with phenotypic and genetic correlations of specific gravity and weight with body weight, weight change, metabolizable energy intake, residual feed consumption, and weight and age at sexual maturity. Data were from 350 White Leghorn hens by 50 sires and 175 dams. Heritabilities of the egg traits ranged from 0.20 to 0.55, increasing with age of bird from 26 to 54 weeks of age. Their standard errors ranged from 0.07 (all data) to 0.17 (26 to 29 weeks). Phenotypic correlations ranged from 0.80 to -0.13, and genetic correlations from 0.91 to -0.27, depending on egg trait. The highest phenotypic...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200008

Hematoxylin: a simple, multiple-use dye for chromosome analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Guerra,Marcelo.
A staining mixture of hematoxylin-iron alum combined with a strong hydrochloric hydrolysis was successfully applied for chromosome observation of several kinds of plants and some animals. Slightly different procedures were developed for different materials and objectives. For plant cells, the most important technical aspect was the use of 5 N HCl hydrolysis, which resulted in a very transparent cytoplasm, combined with an intense, specific hematoxylin stain. This technique is recommended for cytogenetical analysis in general, and it is especially indicated for practical classes, due to its simplicity and high reproducibility of results. Moreover, the deep contrast observed makes this technique very useful for sequential staining of cells previously...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100016

Prevalence of the serpin peptidase inhibitor (alpha-1-antitrypsin) PI*S and PI*Z alleles in Brazilian children with liver disease
Provedor de dados: Genetics and Molecular Biology
Autores: Baldo,Guilherme; Ayala,Ana; Melendez,Matias; Nonnemacher,Karina; Lima,Luciane; Segal,Sandra Leistner; Kieling,Carlos; Vieira,Sandra Gonçalves; Ferreira,Cristina Targa; Silveira,Themis Reverbel da; Giugliani,Roberto; Matte,Ursula.
Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1) deficiency is one of the main genetic causes related to liver disease in children. In SERPINA1 deficiency the most frequent SERPINA1 alleles found are the PI*S and PI*Z alleles. We used the polymerase chain reaction and the amplification created restriction site (ACRS) technique to investigate the prevalence of the PI*S and PI*Z alleles in a group of Brazilian children (n = 200) with liver disease and established the general frequency of the PI*S allele in our population. We found a significant association of the PI*Z allele and liver disease, but no such relationship was found for the PI*S allele. Our results show that SERPINA1 deficiency due to the PI*Z allele,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-1-antitrypsin deficiency; Liver disease; Pediatric patients; SERPINA1.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300005

Short-tandem repeat analysis in seven Chinese regional populations
Provedor de dados: Genetics and Molecular Biology
Autores: Song,Xing-bo; Zhou,Yi; Ying,Bin-wu; Wang,Lan-lan; Li,Yi-song; Liu,Jian-feng; Bai,Xiao-gang; Zhang,Lei; Lu,Xiao-jun; Wang,Jun; Ye,Yuan-xin.
In the present study, we investigated the application of 13 short tandem repeat (STR) loci (D13S317, D7S820, TH01, D16S539, CSFIPO, VWA, D8S1179, TPOX, FGA, D3S1358, D21S11, D18S51 and D5S818) routinely used in forensic analysis, for delineating population relationships among seven human populations representing the two major geographic groups, namely the southern and northern Chinese. The resulting single topology revealed pronounced geographic and population partitioning, consistent with the differences in geographic location, languages and eating habits. These findings suggest that forensic STR loci might be particularly powerful tools in providing the necessary fine resolution for reconstructing recent human evolutionary history.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Forensic medicine; Population genetics; Short-tandem repeat; Human evolutionary history; Genetic distance.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400002

Nuclear anomalies in the buccal cells of calcite factory workers
Provedor de dados: Genetics and Molecular Biology
Autores: Diler,Songül Budak; Ergene,Serap.
The micronucleus (MN) assay on exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans. To determine the genotoxic effects of calcite dust that forms during processing, MN assay was carried out in exfoliated buccal cells of 50 (25 smokers and 25 non-smokers) calcite factory workers and 50 (25 smokers and 25 non-smokers) age- and sex-matched control subjects. Frequencies of nuclear abnormalities (NA) other than micronuclei, such as binucleates, karyorrhexis, karyolysis and 'broken eggs', were also evaluated. Micronuclei and the other aforementioned anomalies were analysed by two way analysis of covariance. The linear correlations between the types of micronucleus and nuclear abnormalities were determined by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcite; Exfoliated buccal cells; Micronucleus (MN); Genotoxicity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200028

Plant responses to stresses: role of ascorbate peroxidase in the antioxidant protection
Provedor de dados: Genetics and Molecular Biology
Autores: Caverzan,Andréia; Passaia,Gisele; Rosa,Silvia Barcellos; Ribeiro,Carolina Werner; Lazzarotto,Fernanda; Margis-Pinheiro,Márcia.
When plants are exposed to stressful environmental conditions, the production of Reactive Oxygen Species (ROS) increases and can cause significant damage to the cells. Antioxidant defenses, which can detoxify ROS, are present in plants. A major hydrogen peroxide detoxifying system in plant cells is the ascorbate-glutathione cycle, in which, ascorbate peroxidase (APX) enzymes play a key role catalyzing the conversion of H2O2 into H2O, using ascorbate as a specific electron donor. Different APX isoforms are present in distinct subcellular compartments, such as chloroplasts, mitochondria, peroxisome, and cytosol. The expression of APX genes is regulated in response to biotic and abiotic stresses as well as during plant development. The APX responses are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascorbate peroxidase; Antioxidant system; Reactive oxygen species; Abiotic stress; Mutant plants.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600016

Meiosis in elephant grass (Pennisetum purpureum), pearl millet (Pennisetum glaucum) (Poaceae, Poales) and their interspecific hybrids
Provedor de dados: Genetics and Molecular Biology
Autores: Techio,Vânia Helena; Davide,Lisete Chamma; Pereira,Antônio Vander.
The cultivated and sexually compatible species Pennisetum purpureum (elephant grass, 2n = 4x = 28) and Pennisetum glaucum (pearl millet, 2n = 2x = 14) can undergo hybridization which favors the amplification of their genetic background and the introgression of favorable alleles into breeding programs. The main problem with interspecific hybrids of these species is infertility due to triploidy (2n = 3x = 21). This study describes meiosis in elephant grass x pearl millet hybrids and their progenitors. Panicles were prepared according to the conventional protocol for meiotic studies and Alexander’s stain was used for assessing pollen viability. Pearl millet accessions presented regular meiosis with seven bivalents and high pollen viability. For elephant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meiosis; Pennisetum; Elephant grass; Interspecific hybrids; Pearl millet; Pollen.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200025

Polymorphisms FTO rs9939609, PPARG rs1801282 and ADIPOQ rs4632532 and rs182052 but not lifestyle are associated with obesity related-traits in Mexican children
Provedor de dados: Genetics and Molecular Biology
Autores: Muñoz-Yáñez,C; Pérez-Morales,R; Moreno-Macías,H; Calleros-Rincón,E; Ballesteros,G; González,R. A; Espinosa,J.
Abstract Concerning the genetic factors of obesity, no consistent association between populations has been reported, which may be due to the frequency of polymorphisms, the lifestyle of studied populations and its interaction with other factors. We studied a possible association of polymorphisms FTO rs9939609, PPARG rs1801282, and ADIPOQ rs4632532 and rs182052 with obesity phenotypes in 215 Mexican children. Glucose, triglycerides, cholesterol, HDL and LDL were measured. In addition, weight, height, waist circumference and triceps skin thickness were recorded. High-energy diets and sedentary behavior were evaluated with a validated questionnaire. In contrast with other reports, only FTO rs9939609 was associated with obesity related-traits, including BMI (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Children; Polymorphisms; Energy intake; Physical activity; Lipid profile.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400547

Genetic damage induced by trophic doses of lead in the neotropical fish Hoplias malabaricus (Characiformes, Erythrinidae) as revealed by the comet assay and chromosomal aberrations
Provedor de dados: Genetics and Molecular Biology
Autores: Cestari,Marta Margarete; Lemos,Priscilla Maria M.; Ribeiro,Ciro Alberto de Oliveira; Costa,João Ricardo M. Alves; Pelletier,Emilien; Ferraro,Marcos V.M.; Mantovani,Mário Sérgio; Fenocchio,Alberto Sergio.
The effects of clastogenic or mutagenic agents have rarely been studied in neotropical fish species exposed to contaminated water. In this study, the genetic damage caused by lead in the widely distributed South American fish, Hoplias malabaricus, was assessed using the comet (SCGE) assay and by testing for chromosomal aberrations. Eighteen specimens were acclimatized to laboratory conditions and then chronically exposed to contaminated food by feeding prey (Cyprinus sp.) injected intraperitoneally with doses of inorganic lead adjusted to give a contamination level of 21 mg of Pb2+.g-1 net weight of H. malabaricus. Three fish were sampled for chromosomal analysis after four doses (18 days) and another three after eight doses (41 days) of lead and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal aberrations; Comet assay; Genotoxicity; Hoplias malabaricus; Lead; Mutagenesis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200023

The Brazilian contribution to Attention-Deficit/Hyperactivity Disorder molecular genetics in children and adolescents
Provedor de dados: Genetics and Molecular Biology
Autores: Genro,Júlia Pasqualini; Roman,Tatiana; Rohde,Luis Augusto; Hutz,Mara Helena.
Attention-Deficit/Hyperactivity Disorder (ADHD) is a common psychiatric condition of children worldwide. This disorder is defined by a combination of symptoms of inattention and hyperactivity/impulsivity. Diagnosis is based on a sufficient number of symptoms causing impairment in these two domains determining several problems in personal and academic life. Although genetic and environmental factors are important in ADHD etiology, how these factors influence the brain and consequently behavior is still under debate. It seems to be consensus that a fronto-subcortical dysfunction is responsible, at least in part, for the ADHD phenotype spectrum. The main results from association and pharmacogenetic studies performed in Brazil are discussed. The investigations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ADHD; Candidate genes; Pharmacogenetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600007

Assessment of genetic mutations in the XRCC2 coding region by high resolution melting curve analysis and the risk of differentiated thyroid carcinoma in Iran
Provedor de dados: Genetics and Molecular Biology
Autores: Fayaz,Shima; Fard-Esfahani,Pezhman; Fard-Esfahani,Armaghan; Mostafavi,Ehsan; Meshkani,Reza; Mirmiranpour,Hossein; Khaghani,Shahnaz.
Homologous recombination (HR) is the major pathway for repairing double strand breaks (DSBs) in eukaryotes and XRCC2 is an essential component of the HR repair machinery. To evaluate the potential role of mutations in gene repair by HR in individuals susceptible to differentiated thyroid carcinoma (DTC) we used high resolution melting (HRM) analysis, a recently introduced method for detecting mutations, to examine the entire XRCC2 coding region in an Iranian population. HRM analysis was used to screen for mutations in three XRCC2 coding regions in 50 patients and 50 controls. There was no variation in the HRM curves obtained from the analysis of exons 1 and 2 in the case and control groups. In exon 3, an Arg188His polymorphism (rs3218536) was detected as a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA repair; Gene polymorphism; Mutation analysis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100004

Evidence of geographical structuring in the Malaysian Snakehead, Channa striata based on partial segment of the CO1 gene
Provedor de dados: Genetics and Molecular Biology
Autores: Siti-Balkhis,Abu Bakar; Jamsari,Amirul Firdaus Jamaluddin; Hwai,Tan Shau; Yasin,Zulfigar; Siti-Azizah,Mohd Nor.
Channa striata, locally known as "haruan", is economically important in fisheries and aquaculture industries in several Asian countries. DNA sequencing, based on a partial segment of the Cytochrome oxidase c subunit 1 (CO1) gene, was used to determine genetic variation in C. striata samples from four different populations on the west coast of Peninsular Malaysia. The highest nucleotide and haplotype diversities were observed in the Linggi population (π = 0.0067, h = 0.835), and the lowest in the Timah Tasoh population (π = 0.0008, h = 0.286). Apart from Kajang-Linggi, which was insignificant, F ST values were significant (p &lt; 0.05) in all pairwise-population comparisons. Consequently, it is inferred that genetic structuring C. striata populations in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Channa striata; Snakehead; MtDNA; CO1; Isolation by distance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300024

Genome-wide analysis of EgEVE_1, a transcriptionally active endogenous viral element associated to small RNAs in Eucalyptus genomes
Provedor de dados: Genetics and Molecular Biology
Autores: Marcon,Helena Sanches; Costa-Silva,Juliana; Lorenzetti,Alan Péricles Rodrigues; Marino,Celso Luis; Domingues,Douglas Silva.
Abstract Endogenous viral elements (EVEs) are the result of heritable horizontal gene transfer from viruses to hosts. In the last years, several EVE integration events were reported in plants by the exponential availability of sequenced genomes. Eucalyptus grandis is a forest tree species with a sequenced genome that is poorly studied in terms of evolution and mobile genetic elements composition. Here we report the characterization of E. grandis endogenous viral element 1 (EgEVE_1), a transcriptionally active EVE with a size of 5,664 bp. Phylogenetic analysis and genomic distribution demonstrated that EgEVE_1 is a newly described member of the Caulimoviridae family, distinct from the recently characterized plant Florendoviruses. Genomic distribution of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pararetrovirus; Horizontal transfer; Eucalyptus; Caulimovirus; Insertion.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200217

Somaclonal variation in Asparagus officinalis plants regenerated by organogenesis from long-term callus cultures
Provedor de dados: Genetics and Molecular Biology
Autores: Pontaroli,Ana Clara; Camadro,Elsa Lucila.
Somaclonal variation in plants regenerated by organogenesis from long-term cultured calluses of two diploid staminate genotypes of Asparagus officinalis cv. Argenteuil was characterized by plant phenotype, ploidy, meiotic behavior, pollen viability, fruit and seed set, and AFLP profiles. Phenotypic deviations from the donors were detected in foliage color, flower size, and cladode and flower morphology. Ploidy changes were observed in 37.8% of the 37 regenerants studied. Meiotic alterations in 12 out of 21 regenerants included laggards, dicentric bridges, micronuclei, restitution nuclei and polyads. Of the 408 AFLP markers screened in 43 regenerants and the donors, 2.94% showed polymorphism. High pollen viability was observed in the 22 regenerants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Fertility; Plant morphology; Meiotic behavior; Ploidy.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300015

Common N-acetylgalactosamine-6-sulfate sulfatase (GALNS) exon mutations in Brazilian patients with mucopolysaccharidosis IVA (MPS IVA)
Provedor de dados: Genetics and Molecular Biology
Autores: Dieter,Tatiana; Matte,Ursula da Silveira; Schwartz,Ida Vanessa; Tomatsu,Shunji; Giugliani,Roberto.
Morquio A Syndrome (mucopolysaccharidosis IVA - MPS IVA, OMIM# 253000) is an autosomal recessive inborn error of metabolism caused by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS). We investigated five unrelated Brazilian MPS IVA families for mutations in exons 4, 5, 9 and 10 of the GALNS gene. Six out of the 10 mutant alleles were identified. Taken together with a previous study, which included six unrelated families, common mutations among Brazilian patients were p.N164T, p.G116S and p.G301C. Among one hundred control subjects three novel silent mutations were found (p.A107A; GCC -&gt; GCT, p.Y108Y; TAC -&gt; TAT, p.P357P; CCG -&gt; CCA). Screening starting with exons 4, 5, 9, 10 and 11 may be a good strategy for genotyping of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: GALNS mutations; GALNS mutation detection; Mucopolysaccharidosis IVA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400004

Microsatellite analysis to estimate genetic relationships among five bulgarian sheep breeds
Provedor de dados: Genetics and Molecular Biology
Autores: Kusza,Szilvia; Dimov,Doytcho; Nagy,István; Bõsze,Zsuzsanna; Jávor,András; Kukovics,Sándor.
Herein, genetic relationships among five breeds of Bulgarian sheep were estimated using microsatellite markers. The total number of alleles identified was 226 at the 16 loci examined. D A distance values were used for phylogenetic tree construction with the UPGMA algorithm. The two Tsigai and two Maritza populations were found to be geneticallvery closely related to each other y (0.198, and 0.258 respectively). The Pleven Black Head population was distinct from the other four. These results could be useful for preserving genes in these breeds, thereby ensuring their preservation in Bulgaria.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic relationships; Bulgarian sheep breeds; Microsatellite.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100010

Differential expression of metallothionein type-2 homologues in leaves and roots of Black pepper (Piper nigrum L)
Provedor de dados: Genetics and Molecular Biology
Autores: Alex,Susan M.; Dicto,Jose; Purushothama,M.G.; Manjula,S.
Black pepper (Piper nigrum L.), member of the family Piperaceae is indigenous to India and is one of the most widely used spices in the world. In this paper we report the results of our attempts to identify a set of genes differentially expressed in the leaves of Piper nigrum, which could facilitate targeted engineering of this valuable crop. A PCR-based Suppression Subtractive Hybridization (SSH) technique was used to generate a leaf-specific subtracted cDNA library of Piper nigrum. A tester population of leaf cDNA was subtracted with a root derived driver cDNA. The efficiency of subtraction was confirmed by PCR analysis using the housekeeping gene actin. On sequence analysis, almost 30% of the clones showed homology to metallothionein type-2 gene. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piper nigrum; Suppression subtractive hybridization; Metallothionein type-2; Real-time PCR analysis; Northern blotting.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300024

Identification and in silico analysis of the Citrus HSP70 molecular chaperone gene family
Provedor de dados: Genetics and Molecular Biology
Autores: Fietto,Luciano G.; Costa,Maximiller D.L.; Cruz,Cosme D.; Souza,Alessandra A.; Machado,Marcos A.; Fontes,Elizabeth P.B.
The completion of the genome sequencing of the Arabidopsis thaliana model system provided a powerful molecular tool for comparative analysis of gene families present in the genome of economically relevant plant species. In this investigation, we used the sequences of the Arabidopsis Hsp70 gene family to identify and annotate the Citrus Hsp70 genes represented in the CitEST database. Based on sequence comparison analysis, we identified 18 clusters that were further divided into 5 subgroups encoding four mitochondrial mtHsp70s, three plastid csHsp70s, one ER luminal Hsp70 BiP, two HSP110/SSE-related proteins and eight cytosolic Hsp/Hsc70s. We also analyzed the expression profile by digital Northern of each Hsp70 transcript in different organs and in response...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chaperones; Digital northern; Heat shock proteins; Genome; Expressed sequence tags.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500017

Defense-related proteins involved in sugarcane responses to biotic stress
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Thais P.; Dias,Renata O.; Silva-Filho,Marcio C.
Abstract Sugarcane is one of the most important agricultural crops in the world. However, pathogen infection and herbivore attack cause constant losses in yield. Plants respond to pathogen infection by inducing the expression of several protein types, such as glucanases, chitinases, thaumatins, peptidase inhibitors, defensins, catalases and glycoproteins. Proteins induced by pathogenesis are directly or indirectly involved in plant defense, leading to pathogen death or inducing other plant defense responses. Several of these proteins are induced in sugarcane by different pathogens or insects and have antifungal or insecticidal activity. In this review, defense-related proteins in sugarcane are described, with their putative mechanisms of action, pathogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Defense-related protein; PR-protein; Biotic stress; Saccharum spp.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200360

Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Guilherme Messias da; Bione,Edgar Guimarães; Cabral-de-Mello,Diogo Cavalcanti; Moura,Rita de Cássia de; Simões,Zilá Luz Paulino; Souza,Maria José de.
Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy p type in D. nisus and D. semisquamosus and of the Xyr type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome rearrangements; Heterochromatin; Karyotype; NORs; FISH.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200012

Phenotypic plasticity of Myzus persicae (Hemíptera: Aphididae) raised on Brassica oleracea L. var. acephala (kale) and Raphanus sativus L. (radish)
Provedor de dados: Genetics and Molecular Biology
Autores: Peppe,Fernanda Borja; Lomônaco,Cecília.
The study of variability generated by phenotypic plasticity is crucial for predicting evolutionary patterns in insect-plant systems. Given sufficient variation for plasticity, host race formation can be favored and maintained, even simpatrically. The plasticity of size and performance (assessed by the lifetime fitness index r m) of six clones of Myzus persicae was tested, with replicates allowed to develop on two hosts, kale (Brassica oleracea var. acephala) and radish (Raphanus sativus). The clones showed significant variability in their plasticity. Reaction norms varied through generations and negative genetic correlation, although not significant, tend to increase with the duration of host use. The lack of plasticity in lifetime fitness among generalist...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quantitative genetics; Genetic correlation; Morphological plasticity; Phenotypic correlation; Physiological plasticity; Trade-offs.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200012

First genotoxicity study of Paraná river water from Argentina using cells from the clam Corbicula fluminea (Veneroida Corbiculidae) and Chinese hamster (Cricetulus griseus Rodentia, Cricetidae) K1 cells in the comet assay
Provedor de dados: Genetics and Molecular Biology
Autores: Caffetti,Jacqueline D.; Mantovani,Mário S.; Pastori,María C.; Fenocchio,Alberto S.
High concentrations of xenobiotics from urban and industrial wastes have contributed to the contamination of many aquatic environments. We used the comet assay to evaluate the genotoxic potential of water collected from the River Paraná, which receives a great deal of waste, at three points (Puerto Piray, Eldorado and Montecarlo) in the Misiones Province of Argentina. The in vivo comet assay used 40 freshwater clams (Corbicula fluminea) while the in vitro comet assay used Chinese hamster (Cricetulus griseus) K1 cell (CHO-K1) cultures with the mutagen ethyl methanesulfonate (EMS) as the positive control and phosphate buffered saline (PBS) as the negative control. Both assays showed statistically significant differences between the three sampling sites in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomonitoring; Comet assay; Corbícula fluminea; CHO-K1 cells; Genotoxicity; Paraná river.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300026

Change in genetic size of small-closed populations: lessons from a domestic mammal population
Provedor de dados: Genetics and Molecular Biology
Autores: Ghafouri-Kesbi,Farhad.
The aim of this study was to monitor changes in genetic size of a small-closed population of Iranian Zandi sheep, by using pedigree information from animals born between 1991 and 2005. The genetic size was assessed by using measures based on the probability of identity-by-descend of genes (coancestry, f, and effective population size, Ne), as well as measures based on probability of gene origin (effective number of founders, f e, effective number of founder genomes, f g, and effective number of non-founder genomes, f ne). Average coancestry, or the degree of genetic similarity of individuals, increased from 0.81% to 1.44% during the period 1993 to 2005, at the same time that Ne decreased from 263 to 93. The observed trend for f e was irregular throughout...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pedigree; Effective size; Genetic drift; Genetic diversity; Genetic similarity; Sheep.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400011

Prevalence of codon 72 P53 polymorphism in Brazilian women with cervix cancer
Provedor de dados: Genetics and Molecular Biology
Autores: Brenna,Sylvia Michelina Fernandes; Silva,Ismael Dale Cotrin Guerreiro da; Zeferino,Luiz Carlos; Pereira,Julia; Martinez,Edson Zachaione; Syrjänen,Kari Juhani.
The p53 codon 72 polymorphism seems to be associated with HPV-carcinogenesis, although controversial data have been reported. A series of Brazilian women with cervix carcinomas were analyzed. Ninety-nine (67%) of 148 women were found to be homozygous (arg/arg) for the arginine polymorphism, and 49 (33%) were heterozygous (arg/pro). This polymorphism may be an important determinant of the risk for cervix cancer, but does not seem to be sufficient for carcinogenesis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P53 polymorphism; Codon 72; Genetic polymorphism; Cervix cancer; Human papillomavirus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400005

Intraspecific variation and population structure of the Velvetbean Caterpillar, Anticarsia gemmatalis Hübner, 1818 (Insecta: Lepidoptera: Noctuidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Sosa-Gómez,Daniel R.
The velvetbean caterpillar (VBC), Anticarsia gemmatalis Hübner, 1818 (Insecta: Lepidoptera: Noctuidae), is one of the most important New World soybean agro-ecosystems pests, occurring from 40° N in the USA to 39° S in Argentina. Information on the migration patterns of the VBC moth may be important for managing the resistance of VBC populations to insecticides or plants carrying the Bacillus thuringiensis insecticide genes, especially since sedentary populations have a higher potential to became resistant than migratory populations. We studied intraspecific variations of geographically distinct VBC populations in order to determine the genetic distance between them and to assess the variability of VBC populations from near the city of Londrina (Paraná (PR)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular marker; RAPD; Gene flow; Soybean pest; Population structure.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300012

Molecular Analysis of Spinal Muscular Atrophy: a genotyping protocol based on TaqMan® real-time PCR
Provedor de dados: Genetics and Molecular Biology
Autores: Godinho,Fernanda Marques de Souza; Bock,Hugo; Gheno,Tailise Conte; Saraiva-Pereira,Maria Luiza.
Spinal muscular atrophy (SMA) is an autosomal recessive inherited disorder caused by alterations in the survival motor neuron I (SMN1) gene. SMA patients are classified as type I-IV based on severity of symptoms and age of onset. About 95% of SMA cases are caused by the homozygous absence of SMN1 due to gene deletion or conversion into SMN2. PCR-based methods have been widely used in genetic testing for SMA. In this work, we introduce a new approach based on TaqMan® real-time PCR for research and diagnostic settings. DNA samples from 100 individuals with clinical signs and symptoms suggestive of SMA were analyzed. Mutant DNA samples as well as controls were confirmed by DNA sequencing. We detected 58 SMA cases (58.0%) by showing deletion of SMN1 exon 7....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SMA; SMN1 gene; Gene conversion; Molecular analysis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600010

The use of self-incompatibility in the production of hybrid eucalyptus seed by `Aracruz Celulose' in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Junghans,Tatiana Góes; Peters-Robinson,Ingrid; Bertolucci,Fernando L.; Alfenas,Acelino Couto.
Self-incompatibility found in a Eucalyptus grandis clone was used to promote interspecific hybridization between E. grandis and E. urophylla clones. The interspecific hybridization achieved in an open-pollinated commercial seed orchard planted in Espírito Santo, Brazil was evaluated by the multi-locus outcrossing rate (tm) of the seed producing clone, E. grandis. The percentage of outcrossed seeds reached 95.9%. The outcrossing rate of individual trees was quite variable, but was always above 70.0%. Wright's coefficient was negative (F = -0.30) revealing an excess of heterozygous genotypes in the progeny. Genetic parameters confirmed the high degree of hybridization expected in this orchard. The 800-m wide protection belt of native tropical forest that...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300015

bFGF promotes adipocyte differentiation in human mesenchymal stem cells derived from embryonic stem cells
Provedor de dados: Genetics and Molecular Biology
Autores: Song,Xinghui; Li,Yanwei; Chen,Xiao; Yin,Guoli; Huang,Qiong; Chen,Yingying; Xu,Guowei; Wang,Linlin.
In this work we describe the establishment of mesenchymal stem cells (MSCs) derived from embryonic stem cells (ESCs) and the role of bFGF in adipocyte differentiation. The totipotency of ESCs and MSCs was assessed by immunofluorescence staining and RT-PCR of totipotency factors. MSCs were successfully used to induce osteoblasts, chondrocytes and adipocytes. MSCs that differentiated into adipocytes were stimulated with and without bFGF. The OD/DNA (optical density/content of total DNA) and expression levels of the specific adipocyte genes PPARγ2 (peroxisome proliferator activated receptor γ2) and C/EBPs were higher in bFGF cells. Embryonic bodies had a higher adipocyte level compared with cells cultured in plates. These findings indicate that bFGF promotes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryonic stem cells; Mesenchymal stem cells; Fibroblast growth factor; Adipocyte differentiation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100019

Differential transcription of ribosomal cistrons denoting nucleolar dominance in hybrids of Drosophila mulleri and Drosophila navojoa (mulleri complex, Repleta group)
Provedor de dados: Genetics and Molecular Biology
Autores: Commar,Leliane Silva; Bicudo,Hermione E.M.C.; Rahal,Paula; Ceron,Carlos Roberto.
The fruit flies Drosophila mulleri and Drosophila navojoa are included in the mulleri complex of the mulleri subgroup and Repleta group. Although there is no demonstration that interspecific crosses between them occur in nature, they intercross in the laboratory in both cross directions. Previous data have shown the occurrence of nucleolar dominance in interspecific hybrids of some species in the mulleri complex. We investigated nucleolar dominance in D. mulleri/D. navojoa hybrids using the transcription profiles of the rDNA internal transcribed spacer (ITS-1) region. The results showed that the ribosomal cistrons present in the X chromosome and in the microchromosome of D. navojoa are exclusively or preferentially transcribed in these hybrids depending on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila mulleri; D. navojoa; Interspecific hybrids; Nucleolar dominance; RDNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600028

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Luvesuto,Eloize; Freitas,Patrícia Domingues de; Galetti Junior,Pedro Manoel.
The culture of the marine shrimp Litopenaeus vannamei has recently boosted the Brazilian shrimp industry. However, it is well known that selection methods based solely on phenotypic characteristics, a reduced number of breeders and the practice of inbreeding may promote a significant raise in the genetic similarity of the captive populations, leading to greater disease susceptibility and impairing both the growth and final size of the shrimps. We used four microsatellite loci to investigate genetic variation in three generations (F5, F6 and F7) of a closed and reared L. vannamei lineage. Although an accentuated heterozygosis deficit was detected, we also observed that the captive propagation of this lineage did not lead to a significant loss of genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broodstocks; Inbreeding; Penaeids; Selection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600021

Molecular discrimination of pouched four-eyed opossums from the Mamirauá Reserve in the Brazilian Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Nunes,Cláudia; Ayres,Jose Marcio; Sampaio,Iracilda; Schneider,Horacio.
Previous cytochrome B (CytB) mtDNA studies have suggested four species for the opossum genus Philander (four-eyed opossums), three (P. mcilhennyi, P. andersoni and P. opossum) from the Amazon and one (P. frenata) from the Brazilian Atlantic forest. During a faunal survey nine specimens of Philander sp. and four of Didelphis marsupialis were collected in the Mamirauá Sustainable Reserve, Amazonas State, Brazil. Preliminary analyses based on morphology and geographical distributions were not conclusive, suggesting that Philander specimens could belong to either P. andersoni or P. opossum. In order to elucidate the relationship of this taxon to the remaining Amazonian taxa, seven Philander and two Didelphis specimens animals were sequenced for the cytB mtDNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytochrome B; Mamirauá; Molecular phylogeny; MtDNA; Philander; Pouched four-eyed opossum.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200015

Components of variation of polygenic systems with digenic epistasis
Provedor de dados: Genetics and Molecular Biology
Autores: Viana,José Marcelo Soriano.
In this paper an extension of the biometric model of Mather and Jinks for the analysis of variation with digenic epistasis is presented. Epistatic effects can contribute favorably to the determination of the genotypic values of selected individuals or families and of superior hybrids. Selection will be inefficient, however, if there is a large number of interacting genes because the epistatic components of the between-family and within-family genotypic variances are very high compared to the portion attributable to the average effects of genes. Selection tends to be efficient when the number of interacting genes is reduced, but this depends on the magnitude of due to dominance and environmental variances. The dominance component (H) and the epistatic...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400030

FOXP in Tetrapoda: Intrinsically Disordered Regions, Short Linear Motifs and their evolutionary significance
Provedor de dados: Genetics and Molecular Biology
Autores: Viscardi,Lucas Henriques; Tovo-Rodrigues,Luciana; Paré,Pamela; Fagundes,Nelson Jurandi Rosa; Salzano,Francisco Mauro; Paixão-Côrtes,Vanessa Rodrigues; Bau,Claiton Henrique Dotto; Bortolini,Maria Cátira.
Abstract The FOXP subfamily is probably the most extensively characterized subfamily of the forkhead superfamily, playing important roles in development and homeostasis in vertebrates. Intrinsically disorder protein regions (IDRs) are protein segments that exhibit multiple physical interactions and play critical roles in various biological processes, including regulation and signaling. IDRs in proteins may play an important role in the evolvability of genetic systems. In this study, we analyzed 77 orthologous FOXP genes/proteins from Tetrapoda, regarding protein disorder content and evolutionary rate. We also predicted the number and type of short linear motifs (SLIMs) in the IDRs. Similar levels of protein disorder (approximately 70%) were found for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FOXP2; Intrinsically disordered protein regions; Forkhead superfamily; Short linear motif; Molecular evolution.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100181

Sequence characterization of hypervariable regions in the soybean genome: leucine-rich repeats and simple sequence repeats
Provedor de dados: Genetics and Molecular Biology
Autores: Barros,Everaldo G. de; Tingey,Scott; Rafalski,J. Antoni.
The genetic basis of cultivated soybean is rather narrow. This observation has been confirmed by analysis of agronomic traits among different genotypes, and more recently by the use of molecular markers. During the construction of an RFLP soybean map (Glycine soja x Glycine max) the two progenitors were analyzed with over 2,000 probes, of which 25% were polymorphic. Among the probes that revealed polymorphisms, a small proportion, about 0.5%, hybridized to regions that were highly polymorphic. Here we report the sequencing and analysis of five of these probes. Three of the five contain segments that encode leucine-rich repeat (LRR) sequence homologous to known disease resistance genes in plants. Two other probes are relatively AT-rich and contain segments...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200029

Identification of proliferating cells in chicken embryos using 5-bromo- 2'-deoxyuridine immunohistochemical detection
Provedor de dados: Genetics and Molecular Biology
Autores: Lopes,Mário Lúcio; Schmidt,Gilberto Silber; Coutinho,Luiz Lehmann.
Chicken embryos were incubated with BrdU, embedded in plastic resin, sectioned and screened immunohistochemically to identify proliferating cells in the neural tube and somites. Fixation in 4% paraformaldehyde for 1 h was essential for detecting specific colorimetric signals of BrdU incorporation into cells during the S phase of the cell cycle. Transverse sections of the neural tube showed that the nuclei of proliferating cells (BrdU positive) had a uniform and centralized distribution, whereas unstained nuclei were found only along the extremities of the neural tube. Transverse sections of differentiated somites showed proliferating cells in the scleratome and dermatome. However, no incorporation of BrdU was observed in myotomic cells, which give rise to...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100028

Cytogenetics and evolution of cassava (Manihot esculenta Crantz)
Provedor de dados: Genetics and Molecular Biology
Autores: Nassar,Nagib M.A.
All Manihot species so far examined, including cassava (Manihot esculenta), have 2n = 36. Interspecific hybrids between cassava and its wild relatives show fair regular meiosis, and backcrossed generations exhibit high fertility. Electrophoresis shows affinity between species of different sections, as well as between some of them and cassava itself. Polyploidy has apparently contributed to the rapid speciation of this genus, while apomixis has offered a means of perpetuating new hybrid types adapted to different environments. It is assumed that cassava originated by hybridization between two wild Manihot species followed by vegetative reproduction of the hybrid.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400046

Rapid sequence divergence rates in the 5 prime regulatory regions of young Drosophila melanogaster duplicate gene pairs
Provedor de dados: Genetics and Molecular Biology
Autores: Kohn,Michael H.
While it remains a matter of some debate, rapid sequence evolution of the coding sequences of duplicate genes is characteristic for early phases past duplication, but long established duplicates generally evolve under constraint, much like the rest of the coding genome. As for coding sequences, it may be possible to infer evolutionary rate, selection, and constraint via contrasts between duplicate gene divergence in the 5 prime regions and in the corresponding synonymous site divergence in the coding regions. Finding elevated rates for the 5 prime regions of duplicated genes, in addition to the coding regions, would enable statements regarding the early processes of duplicate gene evolution. Here, 1 kb of each of the 5 prime regulatory regions of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene duplication; Gene expression; Selection; Promoter evolution.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300028

Mitochondrial variability in the D-loop of four equine breeds shown by PCR-SSCP analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Mirol,Patricia M.; Peral García,P.; Dulout,F.N.
A fragment of 466 base pairs from a highly variable peripheral region of the mitochondrial D-loop of horses was amplified and analyzed by single stranded conformational polymorphism (SSCP). Fourteen distinct SSCP variants were detected in 100 horses belonging to four breeds (Arabian, ARB; Thoroughbred, TB; Argentinian Creole, ARC; and Peruvian Paso from Argentina, PPA). Each breed showed four to eight SSCP variants, many of which were shared between two or three of the studied breeds. Arabian horses were the most variable (eigth variants), with three variants unique to the breed. PPA and ARC showed two and one characteristic SSCP variants, respectively, while TB shared all its variants with at least one of the other breeds. An analysis based on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MTDNA; Equine; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100006

Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells
Provedor de dados: Genetics and Molecular Biology
Autores: Guterres,Zaira da Rosa; Mantovani,Mário Sérgio; Eira,Augusto Ferreira da; Ribeiro,Lúcia Regina; Jordão,Berenice Quinzani.
Agaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 -&gt; 6)beta-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN) and comet (single cell microgel electrophoresis) assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB) obtained from commercial sources or the following strains: a) strains AB 97/29 (young and sporulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus blazei; Micronuclei; Comet assay; V79 cells; Mushroom extracts.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300022

Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Wendell Jacinto; Melo,Arthur Tavares de Oliveira; Coelho,Alexandre Siqueira Guedes; Rodrigues,Fabiana Aparecida; Mamidi,Sujan; Alencar,Sérgio Amorim de; Lanna,Anna Cristina; Valdisser,Paula Arielle Mendes Ribeiro; Brondani,Claudio; Nascimento-Júnior,Ivanildo Ramalho do; Borba,Tereza Cristina de Oliveira; Vianello,Rosana Pereira.
Abstract Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Water deficit; Functional genomics; Single nucleotide polymorphism (SNP).
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100309

Intra-individual numerical chromosomal polymorphism in Trichomycterus davisi (Siluriformes, Trichomycteridae) from the Iguaçu River basin in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Borin,Luciana Andreia; Martins-Santos,Isabel Cristina.
Cytogenetic analysis of Trichomycterus davisi, collected from the Iguaçu River basin, has shown a diploid number of 2n = 54 chromosomes. However, we observed intra-individual numerical polymorphism in a T. davisi specimen. There were three cell populations with diploid number 2n = 54 (40M + 12SM + 2ST), 2n = 55 (40M + 12SM + 2ST + 1M) and 2n = 56 (40M + 12SM + 2ST + 2A) chromosomes. This variation was attributed to a probable post-zygotic non-disjunction of a metacentric chromosome of small/middle size, followed by centric fission, which originated in this individual.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300018

Chromatin differentiation between Theobroma cacao L. and T. grandiflorum Schum
Provedor de dados: Genetics and Molecular Biology
Autores: Dantas,Liliane G.; Guerra,Marcelo.
A comparative analysis of mitotic chromosomes of Theobroma cacao (cacao) and T. grandiflorum (cupuaçu) was performed aiming to identify cytological differences between the two most important species of this genus. Both species have symmetric karyotypes, with 2n = 20 metacentric chromosomes ranging in size from 2.00 to 1.19 µm (cacao) and from 2.21 to 1.15 mm (cupuaçu). The interphase nuclei of both species were of the arreticulate type, displaying up to 20 chromocentres, which were more regularly shaped in cacao than in cupuaçu. Prophase chromosomes of both species were more condensed in the proximal region, sometimes including the whole short arm. Both species exhibited only one pair of terminal heterochromatic bands, positively stained with chromomycin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cacao; Cupuaçu; Heterochromatin; Karyotype; RDNA sites; Theobroma.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100017

Genotoxic evaluation of the River Paranaíba hydrographic basin in Monte Carmelo, MG, Brazil, by the Tradescantia micronucleus
Provedor de dados: Genetics and Molecular Biology
Autores: Campos,Carlos F.; Pereira,Boscolli B.; Campos-Junior,Edimar O. de; Sousa,Eduardo F.; Souto,Henrique N.; Morelli,Sandra.
Abstract Pollutants have adverse effects on human health and on other organisms that inhabit or use water resources. The aim of the present study was to assess the environmental quality of three watercourses in Monte Carmelo, MG, Brazil, using the micronucleus test on Tradescantia. For each treatment, 15 plants were exposed to water samples for 24 h. The control group was exposed to formaldehyde (0.2%) and the negative control to Hoagland solution. Subsequently the plants were placed in Hoagland solution for 24 h to recover. Cells were stained with 2% acetic carmine and examined by light microscopy. Three hundred tetrads were analyzed per slide. The frequency of genotoxic alterations was expressed as the number of micronuclei per 100 tetrads, and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomonitoring; Genotoxicity; Micronucleus; Tradescantia pallida.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400507

N-glycosylation in sugarcane
Provedor de dados: Genetics and Molecular Biology
Autores: Maia,Ivan G.; Leite,Adilson.
The N-linked glycosylation of secretory and membrane proteins is the most complex posttranslational modification known to occur in eukaryotic cells. It has been shown to play critical roles in modulating protein function. Although this important biological process has been extensively studied in mammals, much less is known about this biosynthetic pathway in plants. The enzymes involved in plant N-glycan biosynthesis and processing are still not well defined and the mechanism of their genetic regulation is almost completely unknown. In this paper we describe our first attempt to understand the N-linked glycosylation mechanism in a plant species by using the data generated by the Sugarcane Expressed Sequence Tag (SUCEST) project. The SUCEST database was...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100030

A comparison of metrics for estimating phylogenetic signal under alternative evolutionary models
Provedor de dados: Genetics and Molecular Biology
Autores: Diniz-Filho,José Alexandre F.; Santos,Thiago; Rangel,Thiago Fernando; Bini,Luis Mauricio.
Several metrics have been developed for estimating phylogenetic signal in comparative data. These may be important both in guiding future studies on correlated evolution and for inferring broad-scale evolutionary and ecological processes (e.g., phylogenetic niche conservatism). Notwithstanding, the validity of some of these metrics is under debate, especially after the development of more sophisticated model-based approaches that estimate departure from particular evolutionary models (i.e., Brownian motion). Here, two of these model-based metrics (Blomberg's K-statistics and Pagel's λ) are compared with three statistical approaches [Moran's I autocorrelation coefficient, coefficients of determination from the autoregressive method (ARM), and phylogenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autocorrelation; Blomberg's K; Pagel's lambda; Autoregressive method; Moran's I; Phylogenetic eigenvector regression.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400019

Identification in silico of putative damage responsive elements (DRE) in promoter regions of the yeast genome
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Walkiria Luckwu de Santana; Cavalcanti,Andre Ricardo de Oliveira; Guimarães,Katia Silva; Morais Jr.,Marcos Antonio de.
We report an in silico analysis to identify nucleotide sequence motifs in DNA repair genes that may define a binding site for regulatory proteins during the induction of those genes by mutagens. The damage responsive elements (DRE) weight matrix generated in this analysis was used to search for homologous sequences in the promoter region of all genes, including putative gene and hypothetical open reading frames (ORFs), in the Saccharomyces Genome Data Base (SGD). The results demonstrated that over one third of the yeast genes in the database presented at least one 15-bp sequence in their promoter region with 85% or more of similarity to the DRE consensus sequence. The presence of the DRE sequence in the promoter region of regulatory genes and its high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA binding site; DNA repair; Gene promoter; Gene regulation; Transfac; Weight matrix.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500025

Molecular and biochemical characterization of carbonic anhydrases of Paracoccidioides
Provedor de dados: Genetics and Molecular Biology
Autores: Tomazett,Mariana Vieira; Zanoelo,Fabiana Fonseca; Bailão,Elisa Flávia Cardoso; Bailão,Alexandre Melo; Borges,Clayton Luiz; Soares,Célia Maria de Almeida.
Abstract Carbonic anhydrases (CA) belong to the family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. In the present work, we characterized the cDNAs of four Paracoccidioides CAs (CA1, CA2, CA3, and CA4). In the presence of CO2, there was not a significant increase in fungal ca1, ca2 and ca4 gene expression. The ca1 transcript was induced during the mycelium-to-yeast transition, while ca2 and ca4 gene expression was much higher in yeast cells, when compared to mycelium and mycelium-to-yeast transition. The ca1 transcript was induced in yeast cells recovered directly from liver and spleen of infected mice, while transcripts for ca2 and ca4 were down-regulated. Recombinant CA1 (rCA1) and CA4 (rCA4), with 33...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbonic anhydrases; Paracoccidioides; CO2; Gene expression; Enzyme assay.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300416

Wild cassava, Manihot spp.: Biology and potentialities for genetic improvement
Provedor de dados: Genetics and Molecular Biology
Autores: Nassar,Nagib M.A.
Wild species of Manihot are progenitors of cassava. They constitute valuable genetic reservoirs presenting genes that show new characters. Screening of these species showed some of them to have a notably high percentage of protein combined with a low percentage of hydrocyanic acid. Study of natural habitats revealed resistance to drought and excessive soil aluminum toxicity as well as adaptation to low temperature. Some of the hybrids obtained showed high root productivity and resistance to stem borers. Apomixis was discovered in the wild and transferred successfully to the cultivate species.
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100035

AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
Provedor de dados: Genetics and Molecular Biology
Autores: Gerhardt,Liliane B. de A.; Magioli,Cláudia; Perez,Ana B.U.C.M.; Margis,Rogério; Sachetto-Martins,Gilberto; Margis-Pinheiro,Márcia.
The expression of AtchitIV gene was analysed in Arabidopsis plants submitted to abiotic stresses. Transcript accumulation was detected in leaves in response to UV light exposure, exogenous salicylic acid administration and wounding. Transgenic Arabidopsis plants carrying AtchitIV promoter::gus fusion also showed differential expression of the reporter gene in response to these treatments. The AtchitIV expression was also analysed during Arabidopsis embryo development. GUS assay demonstrated AtchitIV promoter activation in zygotic embryos from torpedo stage up to full maturation. Promoter deletion analysis indicated that all the 5' cis-acting elements responsible for the specific tissue expression are located in a region of 1083 bp, adjacent to the start of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic stress; Chia4 Arabidopsis thaliana endochitinase; Embryogenesis; Gene expression; Promoter.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100020

Individual expression features of GPX2, NQO1 and SQSTM1 transcript variants induced by hydrogen peroxide treatment in HeLa cells
Provedor de dados: Genetics and Molecular Biology
Autores: Belanova,Anna A.; Smirnov,Dmitry S.; Makarenko,Maxim S.; Belousova,Mariya M.; Mashkina,Elena V.; Aleksandrova,Anzhela A.; Soldatov,Alexander V.; Zolotukhin,Peter V.
Abstract Pathway activity assessment-based approaches are becoming highly influential in various fields of biology and medicine. However, these approaches mostly rely on analysis of mRNA expression, and total mRNA from a given locus is measured in the majority of cases. Notably, a significant portion of protein-coding genes produces more than one transcript. This biological fact is responsible for significant noise when changes in total mRNA transcription of a single gene are analyzed. The NFE2L2/AP-1 pathway is an attractive target for biomedical applications. To date, there is a lack of data regarding the agreement in expression of even classical target genes of this pathway. In the present paper we analyzed whether transcript variants of GPX2, NQO1 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NFE2L2/AP-1 pathway; Interactomics; Transcript variants expression control.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300515

In silico characterization of microsatellites in Eucalyptus spp.: abundance, length variation and transposon associations
Provedor de dados: Genetics and Molecular Biology
Autores: Rabello,Edenilson; Souza,Adriane Nunes de; Saito,Daniel; Tsai,Siu Mui.
This study assessed the abundance of microsatellites, or simple sequence repeats (SSR), in 19 Eucalyptus EST libraries from FORESTs, containing cDNA sequences from five species: E. grandis, E. globulus, E. saligna, E. urophylla and E. camaldulensis. Overall, a total of 11,534 SSRs and 8,447 SSR-containing sequences (25.5% of total ESTs) were identified, with an average of 1 SSR/2.5 kb when considering all motifs and 1 SSR/3.1 kb when mononucleotides were not included. Dimeric repeats were the most abundant (41.03%), followed by trimerics (36.11%) and monomerics (19.59%). The most frequent motifs were A/T (87.24%) for monomerics, AG/CT (94.44%) for dimerics, CCG/CGG (37.87%) for trimerics, AAGG/CCTT (18.75%) for tetramerics, AGAGG/CCTCT (14.04%) for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus; EST; Microsatellite; Simple sequence repeat (SSR); Molecular marker.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400013

Lactate dehydrogenase of Mugil sp. (Mugilidae, Perciformes). Lack of electrokinetic, thermostability and kinetic differences among individuals with different number of scales
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Marcelo dos; Schwantes,Maria Luiza B.; Schwantes,Arno Rudi.
The scale number in lateral sets (SNS) of Mugil sp. (Mugilidae, Perciformes) collected in the lagoon-estuarine region of Cananéia, State of São Paulo ranges from 33 to 39. Electrokinetic, kinetic and thermostability properties of lactate dehydrogenase (LDH) were tested to determine if individuals with different SNS correspond to different species or populations of mullet. As in many other teleosts, LDH-A*, LDH-B*, and LDH-C* loci were detected. Through a two-fold serial dilution method applied to 10 different tissues of Mugil sp., a bidirectionally divergent expression of these loci was suggested. No association among LDH electrophoretic pattern, thermal inactivation, kinetic responses and different SNS was observed. The apparent Km (pyr) values obtained...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100026

Characterization and expression of the ABC family (G group) in ‘Dangshansuli’ pear (Pyrus bretschneideri Rehd.) and its russet mutant
Provedor de dados: Genetics and Molecular Biology
Autores: Hou,Zhaoqi; Jia,Bing; Li,Fei; Liu,Pu; Liu,Li; Ye,Zhenfeng; Zhu,Liwu; Wang,Qi; Heng,Wei.
Abstract The plant genes encoding ABCGs that have been identified to date play a role in suberin formation in response to abiotic and biotic stress. In the present study, 80 ABCG genes were identified in ‘Dangshansuli’ Chinese white pear and designated as PbABCGs. Based on the structural characteristics and phylogenetic analysis, the PbABCG family genes could be classified into seven main groups: classes A-G. Segmental and dispersed duplications were the primary forces underlying the PbABCG gene family expansion in ‘Dangshansuli’ pear. Most of the PbABCG duplicated gene pairs date to the recent whole-genome duplication that occurred 30~45 million years ago. Purifying selection has also played a critical role in the evolution of the ABCG genes. Ten PbABCG...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pear; Russet mutant; ABCG; Evolution; Gene expression.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100137

Effect of gamma-radiation and sodium azide on quantitative characters in rice (Oryza sativa L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Montalván,Ricardo; Ando,Akihiko.
Seeds of rice cultivar IAC-1246 received single and combined treatments of 10 or 20 Krad gamma-rays and 0.5 mM sodium azide (SA). The experiments were carried out to assess the effect of treatments on the mean and variance in second generation plants of the following quantitative traits: number of days to flowering (NDF), culm length (CL) and tiller number (TN). In general, the mutagenic treatments increased variance, but did not change the mean for the characters NDF and CL in the M2 generation. There was no increase in the mean or variance of TN. The combined treatments of gamma-rays and SA resulted in larger variance in CL than for the treatments with gamma-rays alone, but not higher than obtained with SA alone. Combined treatments with gamma-rays and...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100014

The complete mitochondrial genome of the small yellow croaker and partitioned Bayesian analysis of Sciaenidae fish phylogeny
Provedor de dados: Genetics and Molecular Biology
Autores: Cheng,Yuanzhi; Wang,Rixin; Sun,Yuena; Xu,Tianjun.
To understand the phylogenetic position of Larimichthys polyactis within the family Sciaenidae and the phylogeny of this family, the organization of the mitochondrial genome of small yellow croaker was determined herein. The complete, 16,470 bp long, mitochondrial genome contains 37 mitochondrial genes (13 protein-coding, 2 ribosomal RNA and 22 transfer RNA genes), as well as a control region (CR), as in other bony fishes. Comparative analysis of initiation/termination codon usage in mitochondrial protein-coding genes of Percoidei species, indicated that COI in Sciaenidae entails an ATG/AGA codon usage different from other Percoidei fishes, where absence of a typical conserved domain or motif in the control regions is common. Partitioned Bayesian analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Larimichthys polyactis; Mitochondrial genome; Control region; Partitioned Bayesian analysis; Sciaenidae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100027

Chromosome study of Anteaters (Myrmecophagideae, Xenarthra): a preliminary report
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira Júnior,Hélio Rubens Jacintho; Jorge,Wilham; Costa,Maria Elvira Loyola Teixeira da.
Anteaters belong to the Order Xenarthra / Family Myrmecophagidae and are the only members without teeth. There are three genera with four living species in the family Myrmecophagidae: Myrmecophaga tridactyla (giant anteater), Tamandua tetradactyla (southern lesser anteater), Tamandua mexicana (northern lesser anteater), and Cyclopes didactylus (silky anteater). The karyotypes of M. tridactyla (2n = 60), T. tetradactyla (2n = 54) and C. didactylus (2n = 64) have already been described. In the present paper, three female and two male specimens of giant anteater and one lesser anteater male were analyzed. The results indicate the existence of a new karyotype in the genus Tamandua, with 2n = 56 chromosomes, which can represent a new lesser anteater species....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anteater; Chromosomes; Myrmecophagidae.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300014

Isoenzymatic variation in the germplasm of Brazilian races of maize (Zea mays L.)
Provedor de dados: Genetics and Molecular Biology
Autores: Gimenes,Marcos Aparecido; Lopes,Catalina Romero.
There are more than 200 races of maize (Zea mays L.) divided into three groups (ancient commercial races, the recent commercial races, and indigenous races). Although the indigenous races have no commercial value, they have many important characteristics which can be incorporated into maize breeding programs. Most Brazilian indigenous germplasm race stocks were collected at least 40 years ago, and nothing is known of the genetic variability present in this germplasm. The genetic variability was assayed in 15 populations from four indigenous races of maize (Caingang, Entrelaçado, Lenha and Moroti) and five indigenous cultivars, using five isoenzymatic systems encoded by 14 loci. The analysis revealed a low level of variability among the samples studied....
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200023

Epigenetic alterations in human brain tumors in a Brazilian population
Provedor de dados: Genetics and Molecular Biology
Autores: Anselmo,Nilson Praia; Bello,Maria Josefa; Gonzalez-Gomez,Pilar; Dias,Luis Antonio Araújo; Almeida,José Reinaldo Walter de; Santos,Marcelo José dos; Rey,Juan A.; Casartelli,Cacilda.
Aberrant methylation of CpG islands located in promoter regions represents one of the major mechanisms for silencing cancer-related genes in tumor cells. We determined the frequency of aberrant CpG island methylation for several tumor-associated genes: DAPK, MGMT, p14ARF, p16INK4a, TP73, RB1 and TIMP-3 in 55 brain tumors, consisting of 26 neuroepithelial tumors, 6 peripheral nerve tumors, 13 meningeal tumors and 10 metastatic brain tumors. Aberrant methylation of at least one of the seven genes studied was detected in 83.6% of the cases. The frequencies of aberrant methylation were: 40% for p14ARF, 38.2% for MGMT, 30.9% for, p16INK4a, 14.6% for TP73 and for TIMP-3, 12.7% for DAPK and 1.8% for RB1. These data suggest that the hypermethylation observed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain tumors; Epigenetics; Methylation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300001

Structural organization of polygalacturonase-encoding genes from Penicillium griseoroseum
Provedor de dados: Genetics and Molecular Biology
Autores: Ribon,Andréa O. B.; Queiroz,Marisa V.; Araújo,Elza F. de.
The pectinolytic system of Penicillium griseoroseum has been studied as a model to investigate aspects of gene organization in filamentous fungi. Here we show that the endopolygalacturonase-coding genes previously isolated exist as single copies in the fungus genome. DNA blot analysis revealed the presence of corresponding genes in other Penicillium species, although only one or two genes were found in opposition to the endoPG gene family reported for other filamentous fungi. The nucleotide and amino acid sequences of Penicillium PG genes of retrieved from data banks were compared for intron length and number, codon usage, and consensus sequences for translation initiation sites. The introns are conserved in the same position, although there was no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicillium griseoroseum; Filamentous fungi; Pectinase; Polygalacturonase; Gene organization.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400020

Editorial
Provedor de dados: Genetics and Molecular Biology
Autores: Foresti,Fausto; Galetti Junior,Pedro Manoel.
Tipo: Info:eu-repo/semantics/other
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200001

Biochemical and molecular investigations on qualitative and quantitative Hb polymorphism in the river buffalo (Bubalus bubalis L.) population reared in Southern Italy
Provedor de dados: Genetics and Molecular Biology
Autores: Iorio,Mario; Vincenti,Donatella; Annunziata,Mario; Rullo,Rosario; Bonamassa,Raffaele; Di Luccia,Aldo; Pieragostini,Elisa.
On 398 river buffalo samples, randomly collected in distinct breeding areas of the Campania region, high-resolution analytical systems were used to identify both qualitative and quantitative variations of the Hb phenotype. Polyacrylamide gel isoelectric focusing and HPLC were used to determine the ratio between HBA1 and HBA2 globin chains; restriction endonuclease analysis was performed to assess whether quantitative variations in Hb bands were related to an unusual number of a-globin genes. In the two buffalo subpopulations, allele frequencies of the alpha and beta globin systems were calculated, and F statistics (FIS, FIT and FST) were estimated as parameters of genetic diversity. The results suggest that: i) as shown by RFLP analysis, only a couple of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenotype; RFLP; Globin genes; Haplotype; Gene frequencies.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200007

Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations
Provedor de dados: Genetics and Molecular Biology
Autores: Tavares,Mara Garcia; Pietrani,Nathalia Teixeira; Durvale,Maxwell de Castro; Resende,Helder Canto; Campos,Lucio Antonio de Oliveira.
Melipona quadrifasciata is a stingless bee widely found throughout the Brazilian territory, with two recognized subspecies, M. quadrifasciata anthidioides, that exhibits interrupted metasomal stripes, and M. quadrifasciata quadrifasciata, with continuous metasomal stripes. This study aimed to estimate the genetic variability of these subspecies. For this purpose, 127 colonies from 15 Brazilian localities were analyzed, using nine species-specific microsatellite primers. At these loci, the number of alleles ranged from three to 15 (mean: 7.2), and the observed heterozygosity (Ho) ranged from 0.03-0.21, while the expected heterozygosity (He) ranged from 0.23-0.47. The genetic distances among populations ranged from 0.03-0.45. The F ST multilocus value (0.23)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic differentiation; Microsatellites; Population genetics; Stingless bees.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100016

Identification of metalloprotease gene families in sugarcane
Provedor de dados: Genetics and Molecular Biology
Autores: Ramos,O.H.P.; Selistre-de-Araujo,H.S.
Metalloproteases play a key role in many physiological processes in mammals such as cell migration, tissue remodeling and processing of growth factors. They have also been identified as important factors in the patho-physiology of a number of human diseases, including cancer and hypertension. Many bacterial pathogens rely on proteases in order to infect the host. Several classes of metalloproteases have been described in humans, bacteria, snake venoms and insects. However, the presence and characterization of plant metalloproteases have rarely been described in the literature. In our research, we searched the sugarcane expressed sequence tag (SUCEST) DNA library in order to identify, by homology with sequences deposited in other databases, metalloprotease...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100037

Microsatellite markers: what they mean and why they are so useful
Provedor de dados: Genetics and Molecular Biology
Autores: Vieira,Maria Lucia Carneiro; Santini,Luciane; Diniz,Augusto Lima; Munhoz,Carla de Freitas.
Abstract Microsatellites or Single Sequence Repeats (SSRs) are extensively employed in plant genetics studies, using both low and high throughput genotyping approaches. Motivated by the importance of these sequences over the last decades this review aims to address some theoretical aspects of SSRs, including definition, characterization and biological function. The methodologies for the development of SSR loci, genotyping and their applications as molecular markers are also reviewed. Finally, two data surveys are presented. The first was conducted using the main database of Web of Science, prospecting for articles published over the period from 2010 to 2015, resulting in approximately 930 records. The second survey was focused on papers that aimed at SSR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR biological function; Genomic distribution; Genotyping approaches; Molecular marker; Practical utility.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300312

Antigenotoxic effects of Mandevilla velutina (Gentianales, Apocynaceae) crude extract on cyclophosphamide-induced micronuclei in Swiss mice and urethane-induced somatic mutation and recombination in Drosophila melanogaster
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Regildo Márcio Gonçalves da; Sousa,Neila Coelho de; Graf,Ulrich; Spanó,Mário Antônio.
A Mandevilla velutina crude extract was investigated using the mouse micronucleus test (MNT) and the Drosophila melanogaster somatic mutation and recombination test (SMART) using standard (ST) and high bioactivation (HB) crosses. The MNT used 10 mg, 20 mg or 40 mg per 100 g of body weight (bw) of extract with and without 0.2 mg per 100 g bw peritoneal cyclophosphamide. There was no genotoxicity in the negative control or extract only groups and, compared to the cyclophosphamide control, there was a significant reduction in micronucleated polychromatic erythrocytes in all the groups given extract plus cyclophosphamide. For SMART larvae were fed 5 or 10 mg mL-1 of extract for seven days with and without 0.89 mg mL-1 of urethane given on day seven. The ST and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigenotoxicity; Genotoxicity tests; Micronucleus assay; SMART; Wing spot test.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400023

DNA replication stress: oncogenes in the spotlight
Provedor de dados: Genetics and Molecular Biology
Autores: Primo,Luiza M. F.; Teixeira,Leonardo K.
Abstract Precise replication of genetic material is essential to maintain genome stability. DNA replication is a tightly regulated process that ensues faithful copies of DNA molecules to daughter cells during each cell cycle. Perturbation of DNA replication may compromise the transmission of genetic information, leading to DNA damage, mutations, and chromosomal rearrangements. DNA replication stress, also referred to as DNA replicative stress, is defined as the slowing or stalling of replication fork progression during DNA synthesis as a result of different insults. Oncogene activation, one hallmark of cancer, is able to disturb numerous cellular processes, including DNA replication. In fact, extensive work has indicated that oncogene-induced replication...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Cell cycle; DNA replication; Oncogene; Replication stress.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200301

Biosynthesis of secondary metabolites in sugarcane
Provedor de dados: Genetics and Molecular Biology
Autores: França,S.C.; Roberto,P.G.; Marins,M.A.; Puga,R.D.; Rodrigues,A.; Pereira,J.O.
A set of genes related to secondary metabolism was extracted from the sugarcane expressed sequence tag (SUCEST) database and was used to investigate both the gene expression pattern of key enzymes regulating the main biosynthetic secondary metabolism pathways and the major classes of metabolites involved in the response of sugarcane to environmental and developmental cues. The SUCEST database was constructed with tissues in different physiological conditions which had been collected under varied situation of environmental stress. This database allows researchers to identify and characterize the expressed genes of a wide range of putative enzymes able to catalyze steps in the phenylpropanoid, isoprenoid and other pathways of the special metabolic mechanisms...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100032

Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Elizabete Cristina da; McManus,Concepta Margaret; Guimarães,Maria Pia Souza Lima Mattos de Paiva; Gouveia,Aurora M.G.; Facó,Olivardo; Pimentel,Daniel M.; Caetano,Alexandre Rodrigues; Paiva,Samuel Rezende.
Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parentage errors; Animal breeding; Animal genetic resources; Capra hircus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010

Bioinformatics analysis of the gene expression profile in Bladder carcinoma
Provedor de dados: Genetics and Molecular Biology
Autores: Xiao,Jing; Yiqing,Qiu.
Bladder carcinoma, which has the ninth highest incidence among malignant tumors in the world, is a complex, multifactorial disease. The malignant transformation of bladder cells results from DNA mutations and alterations in gene expression levels. In this work, we used a bioinformatics approach to investigate the molecular mechanisms of bladder carcinoma. Biochips downloaded from the Gene Expression Omnibus (GEO) were used to analyze the gene expression profile in urinary bladder cells from individuals with carcinoma. The gene expression profile of normal genomes was used as a control. The analysis of gene expression revealed important alterations in genes involved in biological processes and metabolic pathways. We also identified some small molecules...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bladder carcinoma; Differential expression; Expression profile; Small molecular mimic; Susceptibility forecast.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200021

Analysis of 16S rRNA and mxaF genes reveling insights into Methylobacterium niche-specific plant association
Provedor de dados: Genetics and Molecular Biology
Autores: Dourado,Manuella Nóbrega; Andreote,Fernando Dini; Dini-Andreote,Francisco; Conti,Raphael; Araújo,Janete Magali; Araújo,Welington Luiz.
The genus Methylobacterium comprises pink-pigmented facultative methylotrophic (PPFM) bacteria, known to be an important plant-associated bacterial group. Species of this group, described as plant-nodulating, have the dual capacity of producing cytokinin and enzymes, such as pectinase and cellulase, involved in systemic resistance induction and nitrogen fixation under specific plant environmental conditions. The aim hereby was to evaluate the phylogenetic distribution of Methylobacterium spp. isolates from different host plants. Thus, a comparative analysis between sequences from structural (16S rRNA) and functional mxaF (which codifies for a subunit of the enzyme methanol dehydrogenase) ubiquitous genes, was undertaken. Notably, some Methylobacterium spp....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogenetic diversity; Methylotrofics; PPFM; Plant-bacteria interaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100020

Clinical and molecular characterization of a Brazilian cohort of campomelic dysplasia patients, and identification of seven new SOX9 mutations
Provedor de dados: Genetics and Molecular Biology
Autores: Mattos,Eduardo P.; Sanseverino,Maria Teresa V.; Magalhães,José Antônio A.; Leite,Júlio César L.; Félix,Temis Maria; Todeschini,Luiz Alberto; Cavalcanti,Denise P.; Schüler-Faccini,Lavinia.
Campomelic dysplasia (CD) is an autosomal, dominantly inherited, skeletal abnormality belonging to the subgroup of bent bone dysplasias. In addition to bowed lower limbs, CD typically includes the following: disproportionate short stature, flat face, micrognathia, cleft palate, bell-shaped thorax, and club feet. Up to three quarters of 46, XY individuals may be sex-reversed. Radiological signs include scapular and pubic hypoplasia, narrow iliac wings, spaced ischia, and bowed femora and tibiae. Lethal CD is usually due to heterozygous mutations in SOX9, a major regulator of chondrocytic development. We present a detailed clinical and molecular characterization of nine Brazilian CD patients. Infants were either stillborn (n = 2) or died shortly after birth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campomelic dysplasia; Skeletal dysplasia; Osteochondrodysplasias; SOX9; Prenatal diagnosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100014

DNA without secrets and mysteries: a review of DNA Segredos &amp;amp; Mistérios, by Solange Bento Farah
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Sergio Olavo Pinto da.
Tipo: Info:eu-repo/semantics/article
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600031

Isolation and characterization of genes functionally involved in ovarian development of the giant tiger shrimp Penaeus monodon by suppression subtractive hybridization (SSH)
Provedor de dados: Genetics and Molecular Biology
Autores: Preechaphol,Rachanimuk; Klinbunga,Sirawut; Khamnamtongand,Bavornlak; Menasveta,Piamsak.
Suppression subtractive hybridization (SSH) libraries between cDNA in stages I (previtellogenic) and III (cortical rod) ovaries of the giant tiger shrimp (Penaeus monodon) were established. In all, 452 ESTs were unidirectionally sequenced. Sequence assembly generated 28 contigs and 201 singletons, 109 of which (48.0%) corresponding to known sequences previously deposited in GenBank. Several reproduction-related transcripts were identified. The full-length cDNA of anaphase promoting complex subunit 11 (PmAPC11; 600 bp with an ORF of 255 bp corresponding to a polypeptide of 84 amino acids) and selenoprotein M precursor (PmSePM; 904 bp with an ORF of 396 bp corresponding to a polypeptide of 131 amino acids) were characterized and reported for the first time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EST; SSH; Penaeus monodon; Ovarian development; Semiquantitative RT-PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400014

Structure and stability upon maternal transmission of common and intermediate FMR1 (Fragile X Mental Retardation 1) alleles in a sample of the Brazilian population
Provedor de dados: Genetics and Molecular Biology
Autores: Capelli,Leonardo P.; Mingroni-Netto,Regina C.; Vianna-Morgante,Angela M.
In order to investigate the stability of the FMR1 (Fragile X Mental Retardation 1) alleles from the normal population, when maternally inherited, we analyzed 75 mother-to-son transmissions. Sixty-eight alleles fell within the common range with 20-40 CGG repeats, and seven alleles were intermediate, with 41-48 repeats. No change was observed either in the length or in the structure of these repeats upon transmission. Fifty-three alleles were ascertained in different families, and their size distribution was similar to those described for European and European-derived populations, with three peaks of frequency: 66% of the alleles with (CGG)29, (CGG)30 or (CGG)31, 7.5% with (CGG)20, and 5.7% with (CGG)23. Regarding the AGG interspersion pattern, 69.8% had two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FMR1 gene; CGG repeat; Fragile X.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100002

Identification of the GTPase superfamily in Mycoplasma synoviae and Mycoplasma hyopneumoniae
Provedor de dados: Genetics and Molecular Biology
Autores: Borges,Clayton Luiz; Parente,Juliana Alves; Pereira,Maristela; Soares,Célia Maria de Almeida.
Mycoplasmas are the smallest known prokaryotes with self-replication ability. They are obligate parasites, taking up many molecules of their hosts and acting as pathogens in men, animals, birds and plants. Mycoplasma hyopneumoniae is the infective agent of swine mycoplasmosis and Mycoplasma synoviae is responsible for subclinical upper respiratory infections that may result in airsacculitis and synovitis in chickens and turkeys. These highly infectious organisms present a worldwide distribution and are responsible for major economic problems. Proteins of the GTPase superfamily occur in all domains of life, regulating functions such as protein synthesis, cell cycle and differentiation. Despite their functional diversity, all GTPases are believed to have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; GTPase superfamily; Genome.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200007

In vivo evaluation of the mutagenic potential and phytochemical characterization of oleoresin from Copaifera duckei Dwyer
Provedor de dados: Genetics and Molecular Biology
Autores: Maistro,Edson Luis; Carvalho,José Carlos Tavares; Cascon,Vera; Kaplan,Maria Auxiliadora Coelho.
We characterized the chemical constituents of Copaifera duckei oleoresin and used dermal application to Wistar rats to evaluated its possible mutagenic and cytotoxic activities on peripheral blood reticulocytes and bone marrow cells. Chemical characterization of the oleoresin revealed the presence of sesquiterpene hydrocarbons, an unidentified neutral diterpene and diterpene acids. To evaluate mutagenicity evaluation the rats were treated with 10, 25 and 50% of the LD50 dose of the oleoresin for three consecutive days and peripheral blood collected after 0, 24, 48 and 72 h for micronucleus analysis. The rats were humanly sacrificed 24 hours after the last treatment and chromosome preparations made using standard techniques. At the three concentrations and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copaifera duckei (Caesalpinaceae); Phytochemical characterization; Micronucleus test; Chromosome aberrations; Cytotoxic effect.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500028

Estimation of the proportion of genetic variance explained by molecular markers
Provedor de dados: Genetics and Molecular Biology
Autores: Bearzoti,Eduardo; Vencovsky,Roland.
Estimation of the proportion of genetic variance explained by molecular markers (p) plays an important role in basic studies of quantitative traits, as well as in marker-assisted selection (MAS), if the selection index proposed by Lande and Thompson (Genetics 124: 743-756, 1990) is used. Frequently, the coefficient of determination (R2) is used to account for this proportion. In the present study, a simple estimator of p is presented, which is applicable when a multiple regression approach is used, and progenies are evaluated in replicated trials. The associated sampling distribution was obtained and compared with that of R2. Simulations indicated that, when the number of evaluated progenies is small, the statistics are not satisfactory, in general, due to...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400025

Linkage disequilibrium and diversity for three genomic regions in Azoreans and mainland Portuguese
Provedor de dados: Genetics and Molecular Biology
Autores: Branco,Claudia C.; Pacheco,Paula R.; Cabrol,Ester; Cabral,Rita; Vicente,Astrid M.; Mota-Vieira,Luisa.
Studies on linkage disequilibrium (LD) across the genome and populations have been used in recent years with the main objective of improving gene mapping of complex traits. Here, we characterize the patterns of genetic diversity of HLA loci and evaluate LD (D') extent in three genomic regions: Xq13.3, NRY and HLA. In addition, we examine the distribution of DXS1225-DXS8082 haplotype diversity in Azoreans and mainland Portuguese. Allele distribution has demonstrated that the São Miguel population is genetically very diverse; haplotype analysis revealed 100% discriminatory power for X- and Y-markers and 94.3% for HLA markers. Standardized multiallelic D' in these three genomic regions shows values lower than 0.33, thereby suggesting there is no extensive LD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Linkage disequilibrium; X-chromosome; Y-chromosome; HLA; São Miguel; Azores.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200003

The genome sequence of Dyella jiangningensis FCAV SCS01 from a lignocellulose-decomposing microbial consortium metagenome reveals potential for biotechnological applications
Provedor de dados: Genetics and Molecular Biology
Autores: Desiderato,Joana G.; Alvarenga,Danillo O.; Constancio,Milena T.L.; Alves,Lucia M.C.; Varani,Alessandro M.
Abstract Cellulose and its associated polymers are structural components of the plant cell wall, constituting one of the major sources of carbon and energy in nature. The carbon cycle is dependent on cellulose- and lignin-decomposing microbial communities and their enzymatic systems acting as consortia. These microbial consortia are under constant exploration for their potential biotechnological use. Herein, we describe the characterization of the genome of Dyella jiangningensis FCAV SCS01, recovered from the metagenome of a lignocellulose-degrading microbial consortium, which was isolated from a sugarcane crop soil under mechanical harvesting and covered by decomposing straw. The 4.7 Mbp genome encodes 4,194 proteins, including 36 glycoside hydrolases...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant cell wall polysaccharides; Glycoside hydrolases; Genome annotation; Comparative genomics; Rhodanobacteraceae.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300507

Genome sequence of Shewanella corallii strain A687 isolated from pufferfish (Sphoeroides spengleri)
Provedor de dados: Genetics and Molecular Biology
Autores: Azevedo,Gustavo P.R.; Paz,Pedro H. C da; Mattsson,Hannah K.; Moreira,Ana Paula B.; Leomil,Luciana; Calegário,Gabriela; Appolinario,Luciana; Vidal,Lívia; Silva,Bruno S.; Tonon,Luciane A. Chimetto; Tschoeke,Diogo A.; Garcia,Gizele D.; Thompson,Fabiano L.; Thompson,Cristiane C.
Abstract We present here the genome sequence of Shewanella corallii strain A687 isolated from pufferfish Sphoeroides spengleri (Family Tetraodontidae). The assembly consists of 5,215,037 bp and contains 284 contigs, with a G+C content of 50.3%.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shewanella; Gammaproteobacteria; Genome; Gene prediction; Secondary metabolism.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100401

Variability among inbred lines and RFLP mapping of sunflower isozymes
Provedor de dados: Genetics and Molecular Biology
Autores: Carrera,Alicia D.; Pizarro,G.; Poverene,M.; Feingold,S.; León,A.J.; Berry,S.T.
Eight isozyme systems were used in this study: acid phosphatase (ACP), alcohol dehydrogenase (ADH), esterase (EST), glutamate dehydrogenase (GDH), malate dehydrogenase (MDH), phosphoglucoisomerase (PGI), 6-phosphogluconate dehydrogenase (PGD), and phosphoglucomutase (PGM). The polymorphism of these enzyme systems was studied in 25 elite inbred lines. A total of 19 loci were identified, but only eight of them were polymorphic in the germplasm tested. The polymorphic index for the eight informative markers ranged from 0.08 to 0.57, with a mean value of 0.36. Five isozyme loci were mapped in F2:3 populations with existing RFLP data. Est-1, Gdh-2 and Pgi-2 were mapped to linkage groups 3, 14 and 9, respectively. As in previous reports, an ACP locus and a PGD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isozyme; Polymorphisms; RFLP markers; Sunflower; Linkage map; Helianthus annuus.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100013

Genetic diversity analysis of common beans based on molecular markers
Provedor de dados: Genetics and Molecular Biology
Autores: Gill-Langarica,Homar R.; Muruaga-Martínez,José S.; Vargas-Vázquez,M.L. Patricia; Rosales-Serna,Rigoberto; Mayek-Pérez,Netzahualcoyotl.
A core collection of the common bean (Phaseolus vulgaris L.), representing genetic diversity in the entire Mexican holding, is kept at the INIFAP (Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, Mexico) Germplasm Bank. After evaluation, the genetic structure of this collection (200 accessions) was compared with that of landraces from the states of Oaxaca, Chiapas and Veracruz (10 genotypes from each), as well as a further 10 cultivars, by means of four amplified fragment length polymorphisms (AFLP) +3/+3 primer combinations and seven simple sequence repeats (SSR) loci, in order to define genetic diversity, variability and mutual relationships. Data underwent cluster (UPGMA) and molecular variance (AMOVA) analyses. AFLP analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris L.; Amplified fragment length polymorphisms (AFLP); Germplasm bank; Genetic relationships; Simple sequence repeats (SSR).
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400012

Genetics, surnames, grandparents' nationalities, and ethnic admixture in Southern Brazil: Do the patterns of variation coincide?
Provedor de dados: Genetics and Molecular Biology
Autores: Dornelles,C.L.; Callegari-Jacques,S.M.; Robinson,W.M.; Weimer,T.A.; Franco,M.H.L.P.; Hickmann,A.C.; Geiger,C.J.; Salzano,F.M.
A total of 2,708 individuals from the European-derived population of Rio Grande do Sul, divided into seven mesoregions, and of 226 individuals of similar origin from Santa Catarina were studied. Seventeen protein genetic systems, as well as grandparents' nationalities, individuals' surnames, and interethnic admixture were investigated. The alleles which presented the highest and lowest differences were GLO1*2 (16%) and PGD*A (2%), respectively, but in general no significant genetic differences were found among mesoregions. The values observed were generally those expected for individuals of European descent, with the largest difference being a lower prevalence (34-39%) of P*1. Significant heterogeneity among mesoregions was observed for the other variables...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200003

Modulatory effects of Tabebuia impetiginosa (Lamiales, Bignoniaceae) on doxorubicin-induced somatic mutation and recombination in Drosophila melanogaster
Provedor de dados: Genetics and Molecular Biology
Autores: Sousa,Neila C. de; Rezende,Alexandre A.A. de; Silva,Regildo M.G. da; Guterres,Zaira R.; Graf,Ulrich; Kerr,Warwick E.; Spanó,Mário A.
The wing Somatic Mutation and Recombination Test (SMART) in D. melanogaster was used to study genotoxicity of the medicinal plant Tabebuia impetiginosa. Lapachol (naphthoquinone) and β-lapachone (quinone) are the two main chemical constituents of T. impetiginosa. These compounds have several biological properties. They induce apoptosis by generating oxygen-reactive species, thereby inhibiting topoisomerases (I and II) or inducing other enzymes dependent on NAD(P)H:quinone oxidoreductase 1, thus affecting cell cycle checkpoints. The SMART was used in the standard (ST) version, which has normal levels of cytochrome P450 (CYP) enzymes, to check the direct action of this compound, and in the high bioactivation (HB) version, which has a high constitutive level...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Synergistic effect; Somatic mutation and recombination test - SMART; Toxicity; Wing spot test.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200027

Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses
Provedor de dados: Genetics and Molecular Biology
Autores: Poletto,Andréia B.; Wasko,Adriane P.; Oliveira,Claudio; Azevedo,Alexandre; Carvalho,Robson F.; Silva,Maeli Dal Pai; Foresti,Fausto; Martins,Cesar.
Actin-encoding cDNAs of Nile tilapia (Oreochromis niloticus) were isolated by RT-PCR using total RNA samples of different tissues and further characterized by nucleotide sequencing and in silico amino acid (aa) sequence analysis. Comparisons among the actin gene sequences of O. niloticus and those of other species evidenced that the isolated genes present a high similarity to other fish and other vertebrate actin genes. The highest nucleotide resemblance was observed between O. niloticus and O. mossambicus a-actin and b-actin genes. Analysis of the predicted aa sequences revealed two distinct types of cytoplasmic actins, one cardiac muscle actin type and one skeletal muscle actin type that were expressed in different tissues of Nile tilapia. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actin; Expression pattern; Nile tilapia; Oreochromis niloticus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200031

STGC3 inhibits xenograft tumor growth of nasopharyngeal carcinoma cells by altering the expression of proteins associated with apoptosis
Provedor de dados: Genetics and Molecular Biology
Autores: Qiu,Qing-chao; Hu,Bo; He,Xiu-pei; Luo,Qiao; Tang,Guo-hua; Long,Zhi-feng; Chen,Zhu-chu; He,Xiu-sheng.
STGC3 is a potential tumor suppressor that inhibits the growth of the nasopharyngeal carcinoma cell line CNE2; the expression of this protein is reduced in nasopharyngeal carcinoma compared with normal nasopharyngeal tissue. In this study, we investigated the tumor-suppressing activity of STGC3 in nude mice injected subcutaneously with Tet/pTRE-STGC3/CNE2 cells. STGC3 expression was induced by the intraperitoneal injection of doxycycline (Dox). The volume mean of Tet/pTRE-STGC3/CNE2+Dox xenografts was smaller than that of Tet/pTRE/CNE2+Dox xenografts. In addition, Tet/pTRE-STGC3/CNE2+Dox xenografts showed an increase in the percentage of apoptotic cells, a decrease in Bcl-2 protein expression and an increase in Bax protein expression. A proteomic approach...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CNE2 cell line; Nasopharyngeal carcinoma; Nude mouse; STGC3; Tet-on; Two-dimensional electrophoresis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100002

Ammopiptanthus mongolicus stress-responsive NAC gene enhances the tolerance of transgenic Arabidopsis thaliana to drought and cold stresses
Provedor de dados: Genetics and Molecular Biology
Autores: Pang,Xinyue; Xue,Min; Ren,Meiyan; Nan,Dina; Wu,Yaqi; Guo,Huiqin.
Abstract Drought and cold are the primary factors limiting plant growth worldwide. The Ammopiptanthus mongolicus NAC11 (AmNAC11) gene encodes a stress-responsive transcription factor. Expression of the AmNAC11 gene was induced by drought, cold and high salinity. The AmNAC11 protein was localized in the nucleus and plays an important role in tolerance to drought, cold and salt stresses. We also found that differential expression of AmNAC11 was induced in the early stages of seed germination and was related to root growth. When the AmNAC11 gene was introduced into Arabidopsis thaliana by an Agrobacterium-mediated method, the transgenic lines expressing AmNAC11 displayed significantly enhanced tolerance to drought and freezing stresses compared to wild-type...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ammopiptanthus mongolicus; Expression; NAC; Tolerance; Transgene.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400624

Influence of novobiocin on g-irradiation G0-lymphocytes as analyzed by cytogenetic endpoints
Provedor de dados: Genetics and Molecular Biology
Autores: Savoldi-Barbosa,Marcela; Sakamoto-Hojo,Elza T.; Takahashi,Catarina S.
Experiments with novobiocin (NB) post-treatment were performed to verify its effect on the frequencies of micronuclei (MN) and chromosomal aberrations (CA) induced by &lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;-irradiation (0.75, 1.5 and 3.0 Gy) in human lymphocytes at G0-phase. The frequencies of MN significantly decreased by 44 and 50%, for the treatment with NB 50 µg/ml (30-min pulse) after radiation doses of 1.5 and 3.0 Gy, respectively. However, CA frequencies were not significantly affected. No significant effect on CA was observed when lymphocyte cultures were exposed to a single dose of 2.0 Gy at the G0-phase and posttreated with 25 µg/ml NB for three hours either immediately after irradiation (G0-phase) or after 24 h (S-phase). The significant...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200014

High genetic diversity among and within bitter manioc varieties cultivated in different soil types in Central Amazonia
Provedor de dados: Genetics and Molecular Biology
Autores: Alves-Pereira,Alessandro; Peroni,Nivaldo; Cavallari,Marcelo Mattos; Lemes,Maristerra R.; Zucchi,Maria Imaculada; Clement,Charles R.
Abstract Although manioc is well adapted to nutrient-poor Oxisols of Amazonia, ethnobotanical observations show that bitter manioc is also frequently cultivated in the highly fertile soils of the floodplains and Amazonian dark earths (ADE) along the middle Madeira River. Because different sets of varieties are grown in each soil type, and there are agronomic similarities between ADE and floodplain varieties, it was hypothesized that varieties grown in ADE and floodplain were more closely related to each other than either is to varieties grown in Oxisols. We tested this hypothesis evaluating the intra-varietal genetic diversity and the genetic relationships among manioc varieties commonly cultivated in Oxisols, ADE and floodplain soils. Genetic results did...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Microsatellites; Amazonian dark earths; Floodplain; Oxisols.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300468

Identification of photoperception and light signal transduction pathways in citrus
Provedor de dados: Genetics and Molecular Biology
Autores: Quecini,Vera.
Studies employing model species have elucidated several aspects of photoperception and light signal transduction that control plant development. However, the information available for economically important crops is scarce. Citrus genome databases of expressed sequence tags (EST) were investigated in order to identify genes coding for functionally characterized proteins responsible for light-regulated developmental control in model plants. Approximately 176,200 EST sequences from 53 libraries were queried and all bona fide and putative photoreceptor gene families were found in citrus species. We have identified 53 orthologs for several families of transcriptional regulators and cytoplasmic proteins mediating photoreceptor-induced responses although some...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptochrome; Data mining; Light signaling; Phototropin; Phytochrome.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500007

Could human coronavirus OC43 have co-evolved with early humans?
Provedor de dados: Genetics and Molecular Biology
Autores: Brandão,Paulo Eduardo.
Abstract This paper reports on an investigation of the role of codon usage evolution on the suggested bovine-to-human spillover of Bovine coronavirus (BCoV), an enteric/respiratory virus of cattle, resulting in the emergence of the exclusively respiratory Human coronavirus OC43 (HCoV-OC43). Analyses based on full genomes of BCoV and HCoV-OC43 and on both human and bovine mRNAs sequences of cholecystokinin (CCK) and surfactant protein 1 A (SFTP1-A), representing the enteric and respiratory tract codon usage, respectively, have shown natural selection leading to optimization or deoptimization of viral codon usage to the human enteric and respiratory tracts depending on the virus genes under consideration. A higher correlation was found for the nucleotide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Codon usage; Coronavirus; Spillover; Coevolution.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400692

Fragility in the 14q21q translocation region
Provedor de dados: Genetics and Molecular Biology
Autores: Denison,Stacy R.; Multani,Asha S.; Pathak,Sen; Greenbaum,Ira F.
Aphidicolin (APC)-induced chromosomal breakage was analyzed for women representing three generations of a single family and carrying a Robertsonian translocation rob(14q21q). Fluorescence in situ hybridization (FISH) analysis confirmed the dicentric constitution of the derived chromosome and indicated the absence of beta-satellite signal at the translocation region. Per-individual analysis of metaphases from APC-treated peripheral blood lymphocyte cultures identified significantly nonrandom chromosomal breakage at the translocation region in all three individuals examined. The APC-inducible fragility at the 14q21q translocation region suggests that this rearrangement was the result of chromosomal mutation at fragile site(s) in the progenitor chromosomes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rob(14q21q); Robertsonian translocation; Fragile sites; Aphidicolin; Heritability.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300003

Evaluating enzyme replacement therapies for Anderson-Fabry disease: commentary on a recent report
Provedor de dados: Genetics and Molecular Biology
Autores: Giugliani,Roberto; Westwood,Stephanie; Wellhoefer,Hartmann; Schenk,Jörn; Gurevich,Andrey; Kampmann,Christoph.
Abstract Anderson-Fabry disease (AFD) is a rare lysosomal storage disorder. Randomized controlled clinical trials (RCTs) are preferred as the highest category of evidence, but limited availability of robust evidence in rare diseases may necessitate the use of less rigorous evidence. An analysis of cohort studies of enzyme replacement therapies for AFD published in 2017 by El Dib and coworkers made treatment recommendations that contradict previously published findings from RCTs and a systematic Cochrane review. Our commentary outlines concerns regarding selection criteria and statistical methods with their analysis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agalsidase alfa; Agalsidase beta; Anderson-Fabry disease; Enzyme replacement therapy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500790

Karyotypes and heterochromatin variation (C-bands) in Melipona species (Hymenoptera, Apidae, Meliponinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Rocha,Marla Piumbini; Pompolo,Silvia das Graças.
We describe the karyotypes of eight bee species of the genus Melipona and compare them in terms of heterochromatin content and location (C-banding technique). All species had 2n = 18 (females) and n = 9 (males) chromosomes, but a wide variation in heterochromatin content was detected among karyotypes. On the basis of these differences, the species were divided into two functional groups, one of them comprising species with a karyotype having a low heterochromatin content (M. bicolor bicolor, M. quadrifasciata, M. marginata, and M. asilvai), and the other species with a high heterochromatin content (M. seminigra fuscopilosa, M. capixaba, M. scutellaris, and M. captiosa). In the species with high heterochromatin content, heterochromatin occupied practically...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100008

Familial Dysautonomia: Mechanisms and Models
Provedor de dados: Genetics and Molecular Biology
Autores: Dietrich,Paula; Dragatsis,Ioannis.
Abstract Hereditary Sensory and Autonomic Neuropathies (HSANs) compose a heterogeneous group of genetic disorders characterized by sensory and autonomic dysfunctions. Familial Dysautonomia (FD), also known as HSAN III, is an autosomal recessive disorder that affects 1/3,600 live births in the Ashkenazi Jewish population. The major features of the disease are already present at birth and are attributed to abnormal development and progressive degeneration of the sensory and autonomic nervous systems. Despite clinical interventions, the disease is inevitably fatal. FD is caused by a point mutation in intron 20 of the IKBKAP gene that results in severe reduction in expression of IKAP, its encoded protein. In vitro and in vivo studies have shown that IKAP is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Familial Dysautonomia; HSAN; IKAP; Ikbkap; ELP1.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400497

Assessment of the association between SMAD1 and HHIP gene variation and non-syndromic cleft-lip palate in Chilean case-parent trios
Provedor de dados: Genetics and Molecular Biology
Autores: Suazo,José; Santos,José Luis; Jara,Lilian; Blanco,Rafael.
Nonsyndromic cleft lip/palate (NSCLP) is a congenital malformation with features of a complex genetic trait. Several studies have reported positive association and linkage between NSCLP and microsatellite markers in the 4q28-4q33 region particularly with the D4S192 (4q31) marker. We hypothesized that the candidate genes SMAD1 and HHIP (4q31) could be involved in the etiology of NSCLP based on previous positive linkage results and their important role in maxillofacial development. We evaluated the possible association between microsatellite markers located at less than 1 cM from these genes and NSCLP using a sample of 58 Chilean case-parent trios. Microsatellite markers were analyzed using the polymerase chain reaction (PCR) with fluorescent labeled...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candidate genes; Case-parents trio design; Linkage disequilibrium; Nonsyndromic cleft lip/palate.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400006

Genetic changes in grapevine genomes after stress induced by in vitro cultivation, thermotherapy and virus infection, as revealed by AFLP
Provedor de dados: Genetics and Molecular Biology
Autores: Baránek,Miroslav; Raddová,Jana; Krizan,Bretislav; Pidra,Miroslav.
The Amplification Fragment Length Polymorphism (AFLP) technique was employed to study genetic variations which can be induced in vines by the stress occurring during different aspects of viticulture (in vitro cultivation, in vitro thermotherapy and virus infection). Analysis of AFLP banding patterns, generated by using 15 primer combinations, pointed to negligible genetic variation among plants exposed to individual stress. The average of similarity coefficients between differently stressed plants of the cultivars Müller Thurgau and Riesling were 0.984 and 0.991, respectively, as revealed by AFLP analysis. The low incidence of observed polymorphism demonstrates the high level of genome uniformity in plants reproduced by in vitro micropropagation via nodes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grapevine; Stress; Somaclonal variation; Virus infection; AFLP.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400026

Detection of determinant genes and diagnostic via Item Response Theory
Provedor de dados: Genetics and Molecular Biology
Autores: Tavares,Héliton Ribeiro; Andrade,Dalton Francisco de; Pereira,Carlos Alberto de Bragança.
This work presents a method to analyze characteristics of a set of genes that can have an influence in a certain anomaly, such as a particular type of cancer. A measure is proposed with the objective of diagnosing individuals regarding the anomaly under study and some characteristics of the genes are analyzed. Maximum likelihood equations for general and particular cases are presented.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Item Response Theory (IRT); Predisposition; Propensity; Logistic model.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400033

Monomorphic and polymorphic enzyme genetic markers of the Waiãpi Indians of Amapá and of inhabitants of Manaus, Amazonas
Provedor de dados: Genetics and Molecular Biology
Autores: Mestriner,Moacyr A.; Salzano,Francisco M.
One hundred nineteen Waiãpi Indians and 348 inhabitants of Manaus, Amazonas, were tested using nine enzyme genetic systems in the first population and two in the second. The Waiãpi showed some marked differences compared to South American Indian averages in ACP*A (1% vs. 14 ± 9%), GLO1*1 (9% vs. 30 ± 14%) and ESD*1 (99.6% vs. 70 ± 17%), while ESD*1 and CA2*1 prevalences in the Manaus population were within the range previously found in the northern region of Brazil (ESD*1: 85% vs. 80-94%; CA2*1: 98% vs. 89-99.8%). The almost identical frequencies obtained for these two markers in this population, when the sample was subdivided according to skin color, suggest that such morphological classifications have little value in this region. Genetic distances...
Tipo: Info:eu-repo/semantics/other
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300003

Fast detection of deletion breakpoints using quantitative PCR
Provedor de dados: Genetics and Molecular Biology
Autores: Abildinova,Gulshara; Abdrakhmanova,Zhanara; Tuchinsky,Helena; Nesher,Elimelech; Pinhasov,Albert; Raskin,Leon.
Abstract The routine detection of large and medium copy number variants (CNVs) is well established. Hemizygotic deletions or duplications in the large Duchenne muscular dystrophy DMD gene responsible for Duchenne and Becker muscular dystrophies are routinely identified using multiple ligation probe amplification and array-based comparative genomic hybridization. These methods only map deleted or duplicated exons, without providing the exact location of breakpoints. Commonly used methods for the detection of CNV breakpoints include long-range PCR and primer walking, their success being limited by the deletion size, GC content and presence of DNA repeats. Here, we present a strategy for detecting the breakpoints of medium and large CNVs regardless of their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Deletion boundaries; Deletion breakpoints; DMD gene; Duchenne and Becker muscular dystrophies; Hemizygous deletions; Heterozygous deletions.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300365

State biomarkers for Machado Joseph disease: Validation, feasibility and responsiveness to change
Provedor de dados: Genetics and Molecular Biology
Autores: Furtado,Gabriel Vasata; Oliveira,Camila Maria de; Bolzan,Gabriela; Saute,Jonas Alex Morales; Saraiva-Pereira,Maria Luiza; Jardim,Laura Bannach.
Abstract Machado-Joseph disease (SCA3/MJD) is the most common spinocerebellar ataxia worldwide, and particularly so in Southern Brazil. Due to an expanded polyglutamine at ataxin-3, SCA3/MJD presents a relentless course with no current disease modifying treatment. Clinical scales used to measure SCA3/MJD progression present moderate effect sizes, a major drawback for their use as main outcomes in clinical trials, given the rarity and slow progression of the disease. This limitation might be overcome by finding good surrogate markers. We present here a review of studies on peripheral and neurophysiological markers in SCA3/MJD that can be candidates for state biomarkers. Data on markers already studied were summarized, giving emphasis on validation against...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomarkers; Neurophysiology; Machado-Joseph disease; Spinocerebellar ataxia type 3.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000200238

Genetic architecture of purple pigmentation and tagging of some loci to SSR markers in pearl millet, Pennisetum glaucum (L.) R. Br.
Provedor de dados: Genetics and Molecular Biology
Autores: Varalakshmi,Pusapati; Tavva,Surya S Mohan Dev; Rao,Palakollu V Arjuna; Rao,Muktinutalapati V Subba; Hash,Charles T.
This report describes the construction of integrated genetic maps in pearl millet involving certain purple phenotype and simple sequence repeat (SSR) markers. These maps provide a direct means of implementing DNA marker-assisted selection and of facilitating "map-based cloning" for engineering novel traits. The purple pigmentation of leaf sheath, midrib and leaf margin was inherited together 'en bloc' under the control of a single dominant locus (the 'midrib complex') and was inseparably associated with the locus governing the purple coloration of the internode. The purple panicle was caused by a single dominant locus. Each of the three characters (purple lamina, purple stigma and purple seed) was governed by two complementary loci. One of the two loci...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene tagging; Pearl millet; Pigmentation genetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100015

PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
Provedor de dados: Genetics and Molecular Biology
Autores: Freitas,Patrícia Domingues de; Galetti Junior,Pedro Manoel.
The genetic variation in two farmed strains (F3-Panama and F17-Venezuela) of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean heterozygosity and the frequency of polymorphic loci were determined for both strains. A dendrogram of genetic similarity was produced by UPGMA clustering. The results for three primers (INS, M13, YN73) revealed different levels of genetic variation within the strains. The higher genetic similarity seen within strain F17 was apparently related to inbreeding, although a bottleneck effect could not be discarded. The low level of genetic variability of this strain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Litopenaeus vannamei; Genetic diversity; Shrimp; VNTR; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012

New cases of supernumerary chromosomes in characiform fishes
Provedor de dados: Genetics and Molecular Biology
Autores: Venere,Paulo Cesar; Miyazawa,Carlos Suetoshi; Galetti Junior,Pedro Manoel.
Supernumerary chromosomes were described for five species of Neotropical characiform fishes. These extra chromosomes were small, acrocentric and fully heterochromatic in Leporinus friderici from two different localities as well as in Leporinus sp., but metacentric and fully heterochromatic in Cyphocharax modesta and Prochilodus nigricans. In Characidium cf. zebra, this element was small, acrocentric and euchromatic. GC-rich DNA blocks were observed in the supernumerary chromosome of Leporinus sp. using chromomycin A3. The widespread occurrence of these extra chromosomal elements suggests their independent origins.
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300010

Plant growth-promoting rhizobacteria (PGPR): their potential as antagonists and biocontrol agents
Provedor de dados: Genetics and Molecular Biology
Autores: Beneduzi,Anelise; Ambrosini,Adriana; Passaglia,Luciana M.P.
Bacteria that colonize plant roots and promote plant growth are referred to as plant growth-promoting rhizobacteria (PGPR). PGPR are highly diverse and in this review we focus on rhizobacteria as biocontrol agents. Their effects can occur via local antagonism to soil-borne pathogens or by induction of systemic resistance against pathogens throughout the entire plant. Several substances produced by antagonistic rhizobacteria have been related to pathogen control and indirect promotion of growth in many plants, such as siderophores and antibiotics. Induced systemic resistance (ISR) in plants resembles pathogen-induced systemic acquired resistance (SAR) under conditions where the inducing bacteria and the challenging pathogen remain spatially separated. Both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antagonism; Siderophore; Antibiotic; SAR; ISR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600020

Extinction of mammalian populations in conservation units of the Brazilian Cerrado by inbreeding depression in stochastic environments
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Marcel Müller Fernandes Pereira da; Diniz-Filho,José Alexandre Felizola.
Despite methodological and theoretical advances in conservation genetics, data on genetic variation on broad regional spatial scales are still scarce, leading conservation planners to use general heuristic or simulation models for an integrated analysis of genetic, demographic and landscape parameters. Here, we extended previous results by evaluating spatial patterns of extinction by inbreeding depression under stochastic variation of environments for mammalian populations in 31 conservation units of the Brazilian Cerrado. We observed a large spatial variation of times to extinction, for different conservation units and body-size classes of species. For small-bodied species (500 g), the population times to extinction in the conservation units were usually...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population persistence; Population viability analysis; Mammals; Cerrado; Conservation units.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400030

Genetic variants in the fat mass and obesity-associated (FTO) gene confer risk for extreme obesity and modulate adiposity in a Brazilian population
Provedor de dados: Genetics and Molecular Biology
Autores: Fonseca,Ana Carolina Proença da; Marchesini,Bruna; Zembrzuski,Verônica Marques; Voigt,Danielle Dutra; Ramos,Vivianne Galante; Carneiro,João Regis Ivar; Nogueira Neto,José Firmino; Cabello,Giselda Maria Kalil de; Cabello,Pedro Hernán.
Abstract Obesity is a major public health problem worldwide. It has a complex etiology, influenced by environmental and genetic factors. FTO has been recognized as an important genetic factor for obesity development. This study evaluated the contribution of FTO polymorphisms (rs9939609 and rs17817449) for extreme obesity in terms of the period of obesity onset, anthropometric, and biochemical parameters. The haplotype and the combined effects of FTO risk alleles on obesity susceptibility were evaluated. We investigated 169 normal-weight subjects (body mass index, BMI: 22.8 [21.0; 24.0] kg/m2) and 123 extremely obese individuals (BMI: 47.6 [44.1; 53.1] kg/m2). Genotyping was performed by real time PCR. Our results showed a strong association between FTO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BMI; Extreme obesity; FTO; Haplotype; Polymorphisms.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100111

Distribution of the CCR5delta32 allele (gene variant CCR5) in Rondônia, Western Amazonian region, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Farias,Josileide Duarte de; Santos,Marlene Guimarães; França,Andonai Krauze de; Delani,Daniel; Tada,Mauro Shugiro; Casseb,Almeida Andrade; Simões,Aguinaldo Luiz; Engracia,Vera.
Since around 1723, on the occasion of its initial colonization by Europeans, Rondonia has received successive waves of immigrants. This has been further swelled by individuals from northeastern Brazil, who began entering at the beginning of the twentieth century. The ethnic composition varies across the state according to the various sites of settlement of each wave of immigrants. We analyzed the frequency of the CCR5L32 allele of the CCR5 chemokine receptor, which is considered a Caucasian marker, in five sample sets from the population. Four were collected in Porto Velho, the state capital and the site of several waves of migration. Of these, two, from the Hospital de Base were comprised of HB Mothers and HB Newborns presenting allele frequencies of 3.5%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CCR5; CCR5Δ32; Rondônia; Genetic polymorphism; Allele frequency.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100003

Roles of the mitochondrial replisome in mitochondrial DNA deletion formation
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Marcos T.; Pontes,Carolina de Bovi; Ciesielski,Grzegorz L.
Abstract Mitochondrial DNA (mtDNA) deletions are a common cause of human mitochondrial diseases. Mutations in the genes encoding components of the mitochondrial replisome, such as DNA polymerase gamma (Pol γ) and the mtDNA helicase Twinkle, have been associated with the accumulation of such deletions and the development of pathological conditions in humans. Recently, we demonstrated that changes in the level of wild-type Twinkle promote mtDNA deletions, which implies that not only mutations in, but also dysregulation of the stoichiometry between the replisome components is potentially pathogenic. The mechanism(s) by which alterations to the replisome function generate mtDNA deletions is(are) currently under debate. It is commonly accepted that stalling of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondria; DNA replication; Human diseases; Pol γ; Twinkle.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200307

Polymorphism analysis of the hsp70 stress gene in Broiler chickens (Gallus gallus) of different breeds
Provedor de dados: Genetics and Molecular Biology
Autores: Mazzi,Carmen Maria; Ferro,Jesus Aparecido; Ferro,Maria Inês Tiraboschi; Savino,Vicente José Maria; Coelho,Antonio Augusto Domingos; Macari,Marcos.
The promoter region and the beginning of the coding region of the hsp70 stress gene were analysed in broiler chickens of a commercial breed (Hubbard-Pettersen), a breed selected for weight gain (PP1) and a non-selected breed (naked-neck Label Rouge). The naked neck gene (Naked neck, Na), which reduces feathering in birds and is thus related to heat resistance, was present in both PP1 and Label Rouge breeds. Genomic DNA was restricted with PstI and Southern blotting analysis of the samples revealed the absence of polymorphic sites for that enzyme in the promoter region and beginning of the coding region of the hsp70 gene of studied birds. PCR-SSCP analysis of these regions, however, indicated the presence of polymorphisms in the beginning of the coding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler chickens; Hsp70 gene; Naked neck (Na) gene; Polymorphism; SSCP.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300010

A putative RA-like region in the brain of the scale-backed antbird, Willisornis poecilinotus (Furnariides, Suboscines, Passeriformes, Thamnophilidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Jamily L.R. de; Soares,Fabricio A.; Remedios,Ana C.S.; Thom,Gregory; Wirthlin,Morgan; Aleixo,Alexandre; Schneider,Maria Paula C.; Mello,Claudio V.; Schneider,Patricia N.
The memorization and production of song in songbirds share important parallels with the process of speech acquisition in humans. In songbirds, these processes are dependent on a group of specialized telencephalic nuclei known as the song system: HVC (used as a proper name), RA (robust nucleus of arcopallium), LMAN (lateral magnocellular nucleus of the nidopallium) and striatal Area X. A recent study suggested that the arcopallium of the Sayornis phoebe, a non vocal learner suboscine species, contains a nucleus with some properties similar to those of songbird RA, suggesting that the song system may have been present in the last common ancestor of these groups. Here we report morphological and gene expression evidence that a region with some properties...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vocal learning; RGS4; Song nuclei; In situ hybridization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300249

Whole genome SNPs discovery in Nero Siciliano pig
Provedor de dados: Genetics and Molecular Biology
Autores: D’Alessandro,Enrico; Giosa,Domenico; Sapienza,Irene; Giuffrè,Letterio; Cigliano,Riccardo Aiese; Romeo,Orazio; Zumbo,Alessandro.
Abstract Autochthonous pig breeds represent an important genetic reserve to be utilized mainly for the production of typical products. To explore its genetic variability, here we present for the first time whole genome sequencing data and SNPs discovered in a male domestic Nero Siciliano pig compared to the last pig reference genome Sus scrofa11.1.A total of 346.8 million paired reads were generated by sequencing. After quality control, 99.03% of the reads were mapped to the reference genome, and over 11 million variants were detected.Additionally, we evaluated sequence diversity in 21 fitness-related loci selected based on their biological function and/or their proximity to relevant QTLs. We focused on genes that have been related to environmental...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nero Siciliano pig; Whole genome sequencing; Variant calling; SNPs discovery; Fitness genes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400594

SNP discovery and characterisation in White Rhino (Ceratotherium simum) with application to parentage assignment
Provedor de dados: Genetics and Molecular Biology
Autores: Labuschagne,Christiaan; Dalton,Desiré L.; Grobler,J. Paul; Kotzé,Antoinette.
Abstract The white rhino is one of the great success stories of modern wildlife conservation, growing from as few as 50-100 animals in the 1880s, to approximately 20,000 white rhinoceros remaining today. However, illegal trade in conservational rhinoceros horns is adding constant pressure on remaining populations. Captive management of ex situ populations of endangered species using molecular methods can contribute to improving the management of the species. Here we compare for the first time the utility of 33 Single Nucleotide Polymorphisms (SNPs) and nine microsatellites (MS) in isolation and in combination for assigning parentage in captive White Rhinoceros. We found that a combined dataset of SNPs and microsatellites was most informative with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single nucleotide polymorphisms; Microsatellite markers; White Rhinoceros; Ceratotherium simum.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100084

Evaluation of association of DRD2 TaqIA and -141C InsDel polymorphisms with food intake and anthropometric data in children at the first stages of development
Provedor de dados: Genetics and Molecular Biology
Autores: Feistauer,Vanessa; Vitolo,Márcia R.; Campagnolo,Paula D.B.; Mattevi,Vanessa S.; Almeida,Silvana.
Abstract The reward sensation after food intake may be different between individuals and variants in genes related to the dopaminergic system may indicate a different response in people exposed to the same environmental factors. This study investigated the association of TaqIA (rs1800497) and -141C InsDel (rs1799732) variants in DRD2/ANKK1 gene with food intake and adiposity parameters in a cohort of children. The sample consisted of 270 children followed until 7 to 8 years old. DNA was extracted from blood and polymorphisms were detected by PCR-RFLP analysis. Food intake and nutritional status were compared among individuals with different SNP genotypes. Children carrying the A1 allele (TaqIA) had higher energy of lipid dense foods (LDF) when compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Child obesity; DRD2 polymorphisms; Food intake.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400562

Survey of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) biotypes in Brazil using RAPD markers
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,L.H.C.; Návia,D.; Inglis,P.W.; Oliveira,M.R.V. de.
In 1991, the poinsettia strain, silverleaf whitefly or B biotype of Bemisia tabaci was detected in Brazil. This variant is a far more serious agricultural pest than the previously prevalent non-B (BR) biotype. The correct identification of B. tabaci is problematic since it is highly polymorphic with extreme plasticity in key morphological characters that vary according to the host. RAPD-PCR was used to survey the B biotype and other biotypes of B. tabaci in Brazil. Whiteflies were collected from cultivated plants and weeds from 57 different localities and on 27 distinct crops. RAPD analyses using two selected 10-mer primers reliably identified the BR biotype and the B biotype of B. tabaci and also differentiated other whitefly species. The presence of the...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400012

Differential expression of genes identified from Poncirus trifoliata tissue inoculated with CTV through EST analysis and in silico hybridization
Provedor de dados: Genetics and Molecular Biology
Autores: Cristofani-Yaly,Mariângela; Berger,Irving J.; Targon,Maria Luisa P.N.; Takita,Marco A.; Dorta,Sílvia de O.; Freitas-Astúa,Juliana; Souza,Alessandra A. de; Boscariol-Camargo,Raquel L.; Reis,Marcelo S.; Machado,Marcos A.
Citrus is the most important fruit crop in Brazil and Citrus tristeza virus (CTV) is considered one of the most important pathogens of citrus. Most citrus species and varieties are susceptible to CTV infection. However, Poncirus trifoliata, a close relative of citrus, is resistant to the virus. In order to better understand the responses of citrus plants to the infection of CTV, we constructed expressed sequence tag (EST) libraries with tissues collected from Poncirus trifoliata plants, inoculated or not with Citrus tristeza virus at 90 days after inoculation, grafted on Rangpur lime rootstocks. We generated 17,867 sequence tags from Poncirus trifoliata inoculated (8,926 reads) and not (8,941 reads) with a severe CTV isolate. A total of 2,782 TCs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Disease resistance; Citrus tristeza virus; Biotic stress.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500025

Characterization of the DGAT1 gene in the Indian buffalo (Bubalus bubalis)
Provedor de dados: Genetics and Molecular Biology
Autores: Mishra,Bina; Tantia,Madhu S.; Kumar,S.T. Bharani; Vijh,Ramesh K.
The positional candidate diacylglycerol O-acyltransferase (DGAT1) gene affecting milk fat percentage is reported in Indian buffaloes (Bubalus bubalis). A comparison with Chinese buffalo (Bubalus bubalis) revealed eight exonic single nucleotide polymorphisms (SNPs), five of which were non-synonymous. A total of 19 SNPs were observed among diverse buffalo breeds in India. A Unique 22 base insertion has been reported in the intron between exon ten and eleven.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bubalus bubalis; DGAT1 gene; Diacylglycerol O-acyltransferase; Indian water buffalo; Single nucleotide polymorphisms (SNPs).
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600012

Hierarchical structure of genetic distances: Effects of matrix size, spatial distribution and correlation structure among gene frequencies
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Flávia Melo; Diniz-Filho,José Alexandre Felizola.
Geographic structure of genetic distances among local populations within species, based on allozyme data, has usually been evaluated by estimating genetic distances clustered with hierarchical algorithms, such as the unweighted pair-group method by arithmetic averages (UPGMA). The distortion produced in the clustering process is estimated by the cophenetic correlation coefficient. This hierarchical approach, however, can fail to produce an accurate representation of genetic distances among populations in a low dimensional space, especially when continuous (clinal) or reticulate patterns of variation exist. In the present study, we analyzed 50 genetic distance matrices from the literature, for animal taxa ranging from Platyhelminthes to Mammalia, in order...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200010

Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro culture
Provedor de dados: Genetics and Molecular Biology
Autores: Pratta,Guillermo; Zorzoli,Roxana; Picardi,Liliana Amelia.
The phenotypic stability of morphometric traits in Lycopersicon spp. (stem perimeter at the base, middle and top, and number of flowers per cluster) was measured by multivariate analysis through a progeny test in order to estimate the genetic stability of these traits. Principal components were calculated for two groups of Lycopersicon spp., non-regenerated plants and the progeny of regenerated plants. Analysis of variance was performed to support principal component analysis. Both groups presented similar eigenvalues and eigenvectors, while no significant differences were found between any of the traits studied. These results indicated that the phenotypic structure was the same among the progeny of regenerated and non-regenerated plants, so that no...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200039

Joint analysis of phenotypic and molecular diversity provides new insights on the genetic variability of the brazilian physic nut germplasm bank
Provedor de dados: Genetics and Molecular Biology
Autores: Alves,Alexandre Alonso; Bhering,Leonardo Lopes; Rosado,Tatiana Barbosa; Laviola,Bruno Galvêas; Formighieri,Eduardo Fernandes; Cruz,Cosme Damião.
The genetic variability of the Brazilian physic nut (Jatropha curcas) germplasm bank (117 accessions) was assessed using a combination of phenotypic and molecular data. The joint dissimilarity matrix showed moderate correlation with the original matrices of phenotypic and molecular data. However, the correlation between the phenotypic dissimilarity matrix and the genotypic dissimilarity matrix was low. This finding indicated that molecular markers (RAPD and SSR) did not adequately sample the genomic regions that were relevant for phenotypic differentiation of the accessions. The dissimilarity values of the joint dissimilarity matrix were used to measure phenotypic + molecular diversity. This diversity varied from 0 to 1.29 among the 117 accessions, with an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular variability; Jatropha curcas L.; Phenotypic variability.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300012

Cytogenetic analysis of five Hypostomus species (Siluriformes, Loricariidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Martinez,Emanuel Ricardo Monteiro; Zawadzki,Claudio Henrique; Foresti,Fausto; Oliveira,Claudio.
In this work, we analyzed the karyotypes of five species. Hypostomus cf. heraldoi, from the Mogi-Guaçu River, had 2n = 72 chromosomes, with a nucleolar organizer region (NOR) in one chromosomal pair. Hypostomus regani, from the Mogi-Guaçu River had 2n = 72 chromosomes with NORs in two chromosomal pairs. Hypostomus sp., from the Mogi-Guaçu River basin, had 2n = 68 chromosomes, with NORs in two chromosomal pairs. Hypostomus aff. agna, from Cavalo Stream, had 2n = 74 chromosomes with NORs in two chromosomal pairs. Hypostomus cf. topavae, from Carrapato Stream, had 2n = 80 chromosomes, with NORs in two chromosomal pairs. Hypostomus species showed marked diversity in the karyotypic formula, which suggested the occurrence of several Robertsonian rearrangements...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomes; Evolution; Hypostominae; Neotropical fish; NOR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400006

Salt stress affects mRNA editing in soybean chloroplasts
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Nureyev F.; Fonseca,Guilherme C. da; Kulcheski,Franceli R.; Margis,Rogério.
Abstract Soybean, a crop known by its economic and nutritional importance, has been the subject of several studies that assess the impact and the effective plant responses to abiotic stresses. Salt stress is one of the main environmental stresses and negatively impacts crop growth and yield. In this work, the RNA editing process in the chloroplast of soybean plants was evaluated in response to a salt stress. Bioinformatics approach using sRNA and mRNA libraries were employed to detect specific sites showing differences in editing efficiency. RT-qPCR was used to measure editing efficiency at selected sites. We observed that transcripts of NDHA, NDHB, RPS14 and RPS16 genes presented differences in coverage and editing rates between control and salt-treated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Small RNA; Chloroplast; RNA editing; PPR; Salt stress.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200200

PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach
Provedor de dados: Genetics and Molecular Biology
Autores: Campos,Magnólia A.; Rosa,Daniel D.; Teixeira,Juliana Érika C.; Targon,Maria Luisa P.N.; Souza,Alessandra A.; Paiva,Luciano V.; Stach-Machado,Dagmar R.; Machado,Marcos A.
In silico expression profiles, of the discovered 3,103 citrus ESTs putatively encoding for PR protein families (PR-1 to PR-17), were evaluated using the Brazil citrus genome EST CitEST/database. Hierarchical clustering was displayed to identify similarities in expression patterns among citrus PR-like gene families (PRlgf) in 33 selected cDNA libraries. In this way, PRlgf preferentially expressed by organ and citrus species, and library conditions were highlighted. Changes in expression profiles of clusters for each of the 17 PRlgf expressed in organs infected by pathogens or drought-stressed citrus species were displayed for relative suppression or induction gene expression in relation to the counterpart control. Overall, few PRlgf showed expression 2-fold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Functional genome; Ests; Gene expression profiles; Pathogenesis-related proteins; Defense genes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500020

Natural selection and family X location interaction in the common (dry) bean plant
Provedor de dados: Genetics and Molecular Biology
Autores: Pirola,Luís Henrique; Ramalho,Magno Antonio Patto; Carneiro,José Eustáquio S.; Abreu,Ângela de Fátima Barbosa.
Natural selection takes place while advancing generations of segregant populations of self pollinating species by the population (bulk) method. There is evidence that it maintains the individuals with greater grain yield. The question arises whether natural selection preserves the individuals which are more adapted only to the environment where the generation advance occurred, that is, if it contributes to increasing the genotype x environment interaction in the family assessment. This study was carried out to check this hypothesis in the common bean plant using families derived from a segregating population from a cross between the Carioca MG x ESAL 686 cultivars. The segregating populations increase in homozygosity was obtained by the population (bulk)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotype x environment interaction; Heritability; Common bean; Phaseolus vulgaris L.; Natural selection.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300016

Dose assessment by quantification of chromosome aberrations and micronuclei in peripheral blood lymphocytes from patients exposed to gamma radiation
Provedor de dados: Genetics and Molecular Biology
Autores: Silva-Barbosa,Isvânia; Pereira-Magnata,Símey; Amaral,Ademir; Sotero,Graça; Melo,Homero Cavalcanti.
Scoring of unstable chromosome aberrations (dicentrics, rings and fragments) and micronuclei in circulating lymphocytes are the most extensively studied biological means for estimating individual exposure to ionizing radiation (IR), which can be used as complementary methods to physical dosimetry or when the latter cannot be performed. In this work, the quantification of the frequencies of chromosome aberrations and micronuclei were carried out based on cytogenetic analyses of peripheral blood samples from 5 patients with cervical uterine cancer following radiotherapy in order to evaluate the absorbed dose as a result of partial-body exposure to 60Co source. Blood samples were collected from each patient in three phases of the treatment: before...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome aberrations; Micronuclei; Biodosimetry; Partial-body irradiation; Lymphocyte.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300021

Molecular investigation of tRNA genes integrity and its relation to pathogenicity islands in Shiga toxin-producing Escherichia coli (STEC) strains
Provedor de dados: Genetics and Molecular Biology
Autores: Novais,Rogério Carlos; Chaves,Marcela Cassin; Gonzalez,Alice Gonçalves Martins; Andrade,João Ramos Costa.
tRNA genes are known target sites for the integration of pathogenicity islands (PAI) and other genetic elements, such as bacteriophages, into bacterial genome. In most STEC (Shiga toxin-producing Escherichia coli), the PAI called LEE (locus of enterocyte effacement) is related to bacterial virulence and is mostly associated to the tRNA genes selC and pheU. In this work, we first investigated the relationship of LEE with tRNA genes selC and pheU in 43 STEC strains. We found that 28 strains (65%) had a disrupted selC and/or pheU. Three of these strains (637/1, 650/5 and 654/3) were chosen to be submitted to a RAPD-PCR technique modified by the introduction of specific primers (corresponding to the 5'end of genes selC and pheU) into the reaction, which we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogenicity islands; TRNA; STEC; RAPD-PCR; E.coli.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400020

Individual sensitivity to cytogenetic effects of benzo[alpha]pyrene in cultured human lymphocytes: influence of glutathione S-transferase M1 genotype
Provedor de dados: Genetics and Molecular Biology
Autores: Güven,Gülgün S.; Güven,Mehmet; Onaran,Ilhan; Ulutin,Turgut; Hacihanefioglu,Seniha.
Sister chromatid exchange (SCE) and chromosome aberrations (CA) in peripheral lymphocytes has been widely used in assessing exposure to mutagens and carcinogens. One of the extensively studied genotoxins is benzo[alpha]pyrene (BaP). We studied the ability of BaP to induce SCE and CA in 16 glutathione S-transferase M1 (GSTM1)-positive and 15 GSTM1-null individuals by analyzing 72-h whole-blood lymphocyte cultures, either BaP-untreated (controls) or treated with 5 µM of BaP for 24 or 48 h. There was no differences in the level of BaP-induced chromosomal aberrations between GSTM1-positive or null individuals when the cells were BaP-exposed for 24 h (0.083 ± 0.059 vs. 0.090 ± 0.058) or 48 h (0.092 ± 0.057 vs. 0.096 ± 0.050. The frequency of SCE in controls was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sister chromatid exchanges; Chromosomal aberrations; Benzo[alpha]pyrene; Glutathione S-transferase M1.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100027

The prognostic value of the serum ferritin in a southern Brazilian cohort of patients with Gaucher disease
Provedor de dados: Genetics and Molecular Biology
Autores: Koppe,Tiago; Doneda,Divair; Siebert,Marina; Paskulin,Livia; Camargo,Matheus; Tirelli,Kristiane Michelin; Vairo,Filippo; Daudt,Liane; Schwartz,Ida Vanessa D.
Abstract The clinical utility of serum ferritin as a biomarker of disease severity and prognosis in Gaucher disease (GD) is still debated. Here, we aimed to evaluate ferritin and its relation to clinicolaboratory parameters of GD patients seen at the Reference Center for Gaucher Disease of Rio Grande do Sul, Brazil, so as to gather evidence on the utility of ferritin as a biomarker of this condition. A retrospective chart review was performed collecting pre-and posttreatment data from GD patients. Eighteen patients with ferritin levels available before and after treatment were included in the study. Nine of these participants were males, and seventeen had type I GD. All patients were given either enzyme replacement (n = 16) or substrate reduction therapy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ferritin; Biomarkers; Gaucher disease; Iron metabolism.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100030

Prevalence of βS-globin gene haplotypes, α-thalassemia (3.7 kb deletion) and redox status in patients with sickle cell anemia in the state of Paraná, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Shimauti,Eliana LitsukoTomimatsu; Silva,Danilo Grunig Humberto; Souza,Eniuce Menezes de; Almeida,Eduardo Alves de; Leal,Francismar Prestes; Bonini-Domingos,Claudia Regina.
The aim of this study was to determine the frequency of beta S-globin gene (βS globin) haplotypes and alpha thalassemia with 3.7 kb deletion (−α3.7kb thalassemia) in the northwest region of Paraná state, and to investigate the oxidative and clinical-hematological profile of βS globin carriers in this population. Of the 77 samples analyzed, 17 were Hb SS, 30 were Hb AS and 30 were Hb AA. The βSglobin haplotypes and −α3.7kb thalassemia were identified using polymerase chain reaction.Trolox equivalent antioxidant capacity (TEAC) and lipid peroxidation (LPO) were assessed spectophotometrically. Serum melatonin levels were determined using high-performance liquid chromatography coupled to coulometric electrochemical detection. The haplotype frequencies in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Hemoglobinopathies; Melatonin; Sickle cell disease; Thalassemia.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300316

Clastogenic activity of 2-chlorodeoxyadenosine in mammalian somatic cells
Provedor de dados: Genetics and Molecular Biology
Autores: Antonucci,Gilmara Ausech; Takahashi,Catarina Satie.
The base analogue 2-chlorodeoxyadenosine (2-CdA) used for therapy in chronic resistant and advanced lymphoproliferative disorders, is cytotoxic for both dividing and non-dividing lymphocytes. The present work evaluated the clastogenic potential of this drug in vitro in human lymphocytes in culture and in vivo in BALB/c mice bone marrow cells. In human lymphocytes, the clastogenic effect of 2-CdA was studied in G1, S and G2 phases of the cell cycle, using three different concentrations (10, 20 and 40 mug/mL). The endpoints analyzed included mitotic index (MI), proliferation index (PI), sister chromatid exchange (SCE), and chromosomal aberration (CA). Statistical analysis by a variance (ANOVA) test showed a significant increase (p &lt; 0.05) in CA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human lymphocytes; 2-CdA; Chromosomal aberrations; SCE; Cell cycle.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200027

Unusual intron in the second exon of a Type III polyketide synthase gene of Alpinia calcarata Rosc.
Provedor de dados: Genetics and Molecular Biology
Autores: Radhakrishnan,Edayileveettil K; Varghese,Rintu T; Vasudevan,Soniya E.
Plant phenolic compounds form a valuable resource of secondary metabolites having a broad spectrum of biological activities. Type III polyketide synthases play a key role in the formation of basic structural skeleton of the phenolic compounds. As a group of medicinal plants, PKSs with novel features are expected in the genome of Zingiberaceae. The genomic exploration of PKS in Alpinia calcarata conducted in this study identified the presence of an unusual intron at the region forming the second exon of typical PKSs, forming a gateway information of distribution of novel PKSs in Zingiberaceae.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpinia calcarata; CHS; Type III polyketide synthase; Phylogenetic analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100024

Molecular cloning and expression analysis of a zebrafish novel zinc finger protein gene rnf141
Provedor de dados: Genetics and Molecular Biology
Autores: Deng,Wenqian; Sun,Huaqin; Liu,Yunqiang; Tao,Dachang; Zhang,Sizhong; Ma,Yongxin.
ZNF230 is a novel zinc finger gene cloned by our laboratory. In order to understand the potential functions of this gene in vertebrate development, we cloned the zebrafish orthologue of human ZNF230, named rnf141. The cDNA fragment of rnf141 was obtained by rapid amplification of cDNA ends (RACE). The open reading frame (ORF) encodes a polypeptide of 222 amino acids which shares 75.65% identity with the human ZNF230. RT-PCR analysis in zebrafish embryo and adult tissues revealed that rnf141 transcripts are maternally derived and that rnf141 mRNA has a broad distribution. Zygotic rnf141 message is strongly localized in the central nervous system, as shown by whole-mount in situ hybridization. Knockdown and over expression of rnf141 can induce abnormal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rnf141; Zebrafish (Danio rerio); Development; Zinc finger protein.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300024

Analysis of Africanized honey bee mitochondrial DNA reveals further diversity of origin
Provedor de dados: Genetics and Molecular Biology
Autores: Sheppard,Walter S.; Rinderer,Thomas E.; Garnery,Lionel; Shimanuki,Hachiro.
Within the past 40 years, Africanized honey bees spread from Brazil and now occupy most areas habitable by the species Apis mellifera, from Argentina to the southwestern United States. The primary genetic source for Africanized honey bees is believed to be the sub-Saharan honey bee subspecies A. m. scutellata. Mitochondrial markers common in A. m. scutellata have been used to classify Africanized honey bees in population genetic and physiological studies. Assessment of composite mitochondrial haplotypes from Africanized honey bees, using 4 base recognizing restriction enzymes and COI-COII intergenic spacer length polymorphism, provided evidence for a more diverse mitochondrial heritage. Over 25% of the "African" mtDNA found in Africanized populations in...
Tipo: Info:eu-repo/semantics/other
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100015

Diagnosis of the fragile X syndrome in males using methylation-specific PCR of the FMRI locus
Provedor de dados: Genetics and Molecular Biology
Autores: Pena,Sérgio D.J.; Sturzeneker,Rosane.
We have developed a non-isotopic technique based on methylation-specific PCR (MSP) of the FMR1 promoter for the identification of fragile X full mutations among men. DNA samples were first treated with sodium bisulfite to convert unmethylated, but not methylated, cytosines to uracil, followed by PCR amplification with oligonucleotide primers specific for methylated versus unmethylated DNA. We designed two primer pairs: one produced a 142-bp fragment from the bisulfite-treated methylated CpG island, while the other generated an 84-bp product from the treated non-methylated promoter. In normal males only the 84-bp fragment was seen, while the diagnosis of FRAXA was doubly indicated by the appearance of a 142-bp product together with absence or weak...
Tipo: Info:eu-repo/semantics/other
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200005

Allele frequency distributions of six hypervariable loci (D1S80, APOB, D4S43, vW1, F13A and DYS19) in two African-Brazilian communities from the Amazon region
Provedor de dados: Genetics and Molecular Biology
Autores: Vallinoto,Izaura Maria Vieira Cayres; Vallinoto,Antonio Carlos Rosário; Valente,Cristina Maria Duarte; Guerreiro,João Farias.
The allele frequency distributions of three VNTR (D1S80, APOB and D4S43) and three STR (vW1, F13A1 and DYS19) loci were investigated in two Afro-Brazilian populations from the Amazon: Curiau and Pacoval. Exact tests for population differentiation revealed significant differences in allele frequency between populations only for the D1S80 and APOB loci. A statistically significant deviation from the Hardy-Weinberg equilibrium was observed only in the D1S80 locus of the Pacoval sample. A neighbor-joining tree was constructed based on DA genetic distances of allele frequencies in four Afro-Brazilian populations from the Amazon (Pacoval, Curiau, Trombetas, and Cametá), along with those from Congo, Cameroon, Brazilian Amerindians, and Europeans. This analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA polymorphisms; Afro-Brazilians; Hypervariable loci.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300003

Differential metabolism of Mycoplasma species as revealed by their genomes
Provedor de dados: Genetics and Molecular Biology
Autores: Arraes,Fabricio B.M.; Carvalho,Maria José A. de; Maranhão,Andrea Q.; Brígido,Marcelo M.; Pedrosa,Fábio O.; Felipe,Maria Sueli S.
The annotation and comparative analyses of the genomes of Mycoplasma synoviae and Mycoplasma hyopneumonie, as well as of other Mollicutes (a group of bacteria devoid of a rigid cell wall), has set the grounds for a global understanding of their metabolism and infection mechanisms. According to the annotation data, M. synoviae and M. hyopneumoniae are able to perform glycolytic metabolism, but do not possess the enzymatic machinery for citrate and glyoxylate cycles, gluconeogenesis and the pentose phosphate pathway. Both can synthesize ATP by lactic fermentation, but only M. synoviae can convert acetaldehyde to acetate. Also, our genome analysis revealed that M. synoviae and M. hyopneumoniae are not expected to synthesize polysaccharides, but they can take...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mollicutes; Mycoplasma; Metabolism; Comparative genomics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200004

Dmp53, basket and drICE gene knockdown and polyphenol gallic acid increase life span and locomotor activity in a Drosophila Parkinson's disease model
Provedor de dados: Genetics and Molecular Biology
Autores: Ortega-Arellano,Hector Flavio; Jimenez-Del-Rio,Marlene; Velez-Pardo,Carlos.
Understanding the mechanism(s) by which dopaminergic (DAergic) neurons are eroded in Parkinson's disease (PD) is critical for effective therapeutic strategies. By using the binary tyrosine hydroxylase (TH)-Gal4/UAS-X RNAi Drosophila melanogaster system, we report that Dmp53, basket and drICE gene knockdown in dopaminergic neurons prolong life span (p &lt; 0.05; log-rank test) and locomotor activity (p &lt; 0.05; χ² test) in D. melanogaster lines chronically exposed to (1 mM) paraquat (PQ, oxidative stress (OS) generator) compared to untreated transgenic fly lines. Likewise, knockdown flies displayed higher climbing performance than control flies. Amazingly, gallic acid (GA) significantly protected DAergic neurons, ameliorated life span, and climbing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Basket; Dmp53; Drice; Drosophila; Paraquat.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400020

Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes
Provedor de dados: Genetics and Molecular Biology
Autores: Zaros,Lilian Giotto; Coutinho,Luiz Lehmann; Sider,Lúcia Helena; Medeiros,Henrique Rocha de; Neves,Maria Rosalba Moreira das; Benvenuti,Camila Loures; Navarro,Andrine Maria do Carmo; Vieira,Luiz da Silva.
Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperate regions as models, three reference genes, viz., ribosomal protein LO (RPLO), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and succinate dehydrogenase complex subunit A (SDHA), were investigated in the abomasum, abomasal lymph nodes and small intestine of Brazilian Somalis sheep, either resistant or susceptible to gastrointestinal nematodes infections. Real time PCR was carried out by using SYBR Green I dye, and gene stability was tested by geNorm. RPLO was an ideal reference gene, since its expression was constant across treatments, presented lower variation, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Control genes; Gastrointestinal nematodes; Ovine; Real-time RT-PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300018

Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5
Provedor de dados: Genetics and Molecular Biology
Autores: Güllü,Gökçe; Peker,Irem; Haholu,Aptullah; Eren,Fatih; Küçükodaci,Zafer; Güleç,Bülent; Baloglu,Hüseyin; Erzik,Can; Özer,Ayse; Akkiprik,Mustafa.
The functional role of IGFBP5 in breast cancer is complicated. Experimental and bioinformatics studies have shown that IGFBP5 is targeted by miR-140-5p and miR-193b, although this has not yet been proven in clinical samples. The aim of this study was to evaluate the expression of miR-140-5p and miR-193b in breast cancer and adjacent normal tissue and assess its correlation with IGFBP5 and the clinicopathological characteristics of the tumors. IGFBP5 protein expression was analyzed immunohistochemically and IGFBP5, miR-140 and miR-193b mRNA expression levels were analyzed with real-time RT-PCR. Tumor tissue had higher miR-140-5p expression than adjacent normal tissue (p = 0.015). Samples with no immunohistochemical staining for IGFBP5 showed increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; ER alpha; IGFBP5; Micro RNA; MiR-140; MiR-193b.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100021

Population structures of Brazilian Tall coconut (Cocos nucifera L.) by microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Ribeiro,Francisco E.; Baudouin,Luc; Lebrun,Patricia; Chaves,Lázaro J.; Brondani,Claudio; Zucchi,Maria I.; Vencovsky,Roland.
Coconut palms of the Tall group were introduced to Brazil from the Cape Verde Islands in 1553. The present study sought to evaluate the genetic diversity among and within Brazilian Tall coconut populations. Samples were collected of 195 trees from 10 populations. Genetic diversity was accessed by investigating 13 simple sequence repeats (SSR) loci. This provided a total of 68 alleles, ranging from 2 to 13 alleles per locus, with an average of 5.23. The mean values of gene diversity (He) and observed heterozygosity (Ho) were 0.459 and 0.443, respectively. The genetic differentiation among populations was estimated at θ = 0.1600 and the estimated apparent outcrossing rate was t a = 0.92. Estimates of genetic distances between the populations varied from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coconut; Genetic diversity; Genetic variability; Molecular markers; SSR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400016

Inheritance of rice cold tolerance at the germination stage
Provedor de dados: Genetics and Molecular Biology
Autores: Cruz,Renata Pereira da; Milach,Sandra Cristina Kothe; Federizzi,Luiz Carlos.
Cold tolerance during germination is important for ensuring fast and uniform establishment of a rice crop early in the season. However, evaluation of this trait under field conditions is limited by environmental variation, which makes it difficult to identify genetically superior lines. Evaluation of cold tolerance under controlled temperature conditions may be performed by assessing percentage of reduction in coleoptile length and coleoptile growth. Our study determined the inheritance and heritability of cold tolerance at the germination stage in crosses between six rice genotypes. Diallel analysis showed that while both additive and non-additive gene action were involved, the non-additive action was relatively more important for percentage of reduction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chilling tolerance; Oryza sativa; Rice genetics; Seed germination.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200020

Prospecting sugarcane genes involved in aluminum tolerance
Provedor de dados: Genetics and Molecular Biology
Autores: Drummond,Rodrigo D.; Guimarães,Claudia T.; Felix,Juliana; Ninamango-Cárdenas,Fernando E.; Carneiro,Newton P.; Paiva,Edilson; Menossi,Marcelo.
Aluminum is one of the major factors that affect plant development in acid soils, causing a substantial reduction in yield in many crops. In South America, about 66% of the land surface is made up of acid soils where high aluminum saturation is one of the main limiting factors for agriculture. The biochemical and molecular basis of aluminum tolerance in plants is far from being completely understood despite a growing number of studies, and in the specific case of sugarcane there are virtually no reports on the effects of gene regulation on aluminum stress. The objective of the work presented in this paper was to prospect the sugarcane expressed sequence tag (SUCEST) data bank for sugarcane genes related to several biochemical pathways known to be involved...
Tipo: Info:eu-repo/semantics/article
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100029

Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo
Provedor de dados: Genetics and Molecular Biology
Autores: Matic,Sanja; Stanic,Snezana; Bogojevic,Desanka; Solujic,Slavica; Grdovic,Nevena; Vidakovic,Melita; Mihailovic,Mirjana.
The intention was to evaluate the possible in vivo genotoxic potential in different cell-types, of a methanol extract obtained from the plant stem of Cotinus coggygria Scop., using the sex-linked recessive lethal (or SLRL) test and alkaline comet assay. The SLRL test, revealed the genotoxic effect of this extract in postmeiotic and premeiotic germ-cell lines. The comet assay was carried out on rat liver and bone marrow at 24 and 72 h after intraperitoneal administration. For genotoxic evaluation, three concentrations of the extract were tested, viz., 500, 1000 and 2000 mg/kg body weight (bw), based on the solubility limit of the extract in saline. Comet tail moment and total scores in the group treated with 500 mg/kg bw, 24 and 72 h after treatment, were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Cotinus coggygria; Extract; Genotoxic effect; SLRL.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200022

The use of weighted multiple linear regression to estimate QTL-by-QTL epistatic effects
Provedor de dados: Genetics and Molecular Biology
Autores: Bocianowski,Jan.
Knowledge of the nature and magnitude of gene effects, as well as their contribution to the control of metric traits, is important in formulating efficient breeding programs for the improvement of plant genetics. Information concerning a genetic parameter such as the additive-by-additive epistatic effect can be useful in traditional breeding. This report describes the results obtained by applying weighted multiple linear regression to estimate the parameter connected with an additive-by-additive epistatic interaction. Three weight variants were used: (1) standard weights based on estimated variances, (2) different weights for minimal, maximal and other lines, and (3) different weights for extreme and other lines. The approach described here combines two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biometrical genetics; Epistatic interaction; Homozygous lines; QTL; Simulation study; Weighted regression.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500015

Critical electrolyte concentration of chromatin in polytene chromosomes of Trichosia pubescens (Diptera, Sciaridae)
Provedor de dados: Genetics and Molecular Biology
Autores: Monteiro,Ana Lúcia P.; Mello,Maria Luiza S.
The binding of toluidine blue molecules to nucleic acid phosphates under conditions of competition with Mg2+ ions was studied in Trichosia pubescens polytene chromosome regions differing in composition and organization (RNA-rich areas and puffs, DNA puffs, heterochromatin). The aim was to find variability in the Mg2+ concentration at which metachromasy was completely prevented (= critical electrolyte concentration; CEC) and that could reflect differences at the level of nucleoprotein complexes in particular chromosome regions and developmental phases. Although high CEC values have been found in tightly packed chromatin such as that of heterochromatin zones, the CEC values for the other regions have proven to be affected not only by availability and...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200003

The enigmatic monotypic crab plover Dromas ardeola is closely related to pratincoles and coursers (Aves, Charadriiformes, Glareolidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Sergio L.; Baker,Allan J.
The phylogenetic placement of the monotypic crab plover Dromas ardeola (Aves, Charadriiformes) remains controversial. Phylogenetic analysis of anatomical and behavioral traits using phenetic and cladistic methods of tree inference have resulted in conflicting tree topologies, suggesting a close association of Dromas to members of different suborders and lineages within Charadriiformes. Here, we revisited the issue by applying Bayesian and parsimony methods of tree inference to 2,012 anatomical and 5,183 molecular characters to a set of 22 shorebird genera (including Turnix). Our results suggest that Bayesian analysis of anatomical characters does not resolve the phylogenetic relationship of shorebirds with strong statistical support. In contrast, Bayesian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dromas; Charadriiformes; Bayesian tree inference; Phylogenetics; Systematics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300033

Molecular evolution and functional divergence of alcohol dehydrogenases in animals, fungi and plants
Provedor de dados: Genetics and Molecular Biology
Autores: Thompson,Claudia E.; Freitas,Loreta B.; Salzano,Francisco M.
Abstract Alcohol dehydrogenases belong to the large superfamily of medium-chain dehydrogenases/reductases, which occur throughout the biological world and are involved with many important metabolic routes. We considered the phylogeny of 190 ADH sequences of animals, fungi, and plants. Non-class III Caenorhabditis elegans ADHs were seen closely related to tetrameric fungal ADHs. ADH3 forms a sister group to amphibian, reptilian, avian and mammalian non-class III ADHs. In fishes, two main forms are identified: ADH1 and ADH3, whereas in amphibians there is a new ADH form (ADH8). ADH2 is found in Mammalia and Aves, and they formed a monophyletic group. Additionally, mammalian ADH4 seems to result from an ADH1 duplication, while in Fungi, ADH formed clusters...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycolytic proteins; Molecular evolution; Alcohol dehydrogenase; Functional diversification; Positive selection.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200341

Cytogenetic analysis of three species of Pseudacteon (Diptera, Phoridae) parasitoids of the fire ants using standard and molecular techniques
Provedor de dados: Genetics and Molecular Biology
Autores: Chirino,Mónica G.; Folgarait,Patricia J.; Gilbert,Lawrence E.; Lanzavecchia,Silvia; Papeschi,Alba G.
Pseudacteon flies, parasitoids of worker ants, are being intensively studied as potentially effective agents in the biological control of the invasive pest fire ant genus Solenopsis (Hymenoptera: Formicidae). This is the first attempt to describe the karyotype of P. curvatus Borgmeier, P. nocens Borgmeier and P. tricuspis Borgmeier. The three species possess 2n = 6; chromosomes I and II were metacentric in the three species, but chromosome pair III was subtelocentric in P. curvatus and P. tricuspis, and telocentric in P. nocens. All three species possess a C positive band in chromosome II, lack C positive heterochromatin on chromosome I, and are mostly differentiated with respect to chromosome III. P. curvatus and P. tricuspis possess a C positive band,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudacteon; Karyotype evolution; C-banding; Fluorescent banding; FISH.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400013

Crude alkaloid extract of Rhazya stricta inhibits cell growth and sensitizes human lung cancer cells to cisplatin through induction of apoptosis
Provedor de dados: Genetics and Molecular Biology
Autores: Elkady,Ayman I.
There is an urgent need to improve the clinical management of non-small cell lung cancer (NSCLC), one of the most frequent causes of cancer-related deaths in men and women worldwide. Rhazya stricta, an important medicinal plant used in traditional Oriental medicine, possesses anti-oxidant, anti-carcinogenic and free radical scavenging properties. This study was done to explore the potential anticancer activity of a crude alkaloid extract of R. stricta (CAERS) against the NSCLC line A549. CAERS markedly suppressed the growth of A549 cells and considerably enhanced the anti-proliferative potential of cisplatin. CAERS-mediated inhibition of A549 cell growth correlated with the induction of apoptosis that was accompanied by numerous morphological changes, DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Lung cancer; Medicinal plant; RT-PCR; Western blot.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100003

An analysis of multiplex-PCR in the detection of BCR-ABL transcripts in hematological disorders
Provedor de dados: Genetics and Molecular Biology
Autores: Sastre,Darío A.; Argaraña,Carlos E.; Heller,Viviana B.; Gallo,Mónica; Fernández,Enrique N.; Rodríguez,Cecilia M.
In this work, we describe the advantages of multiplex-PCR in the specific detection of BCR-ABL transcripts in different hematological disorders and its sensitivity compared to nested PCR. Fifty-three patients were studied for the presence of BCR-ABL transcripts: 24 patients with chronic myeloid leukemia (CML), 20 with acute leukemia (AL), and 9 patients with other hematological disorders. A variant rearrangement (b3a3) was found in a single case of CML (4.2%). Four out of the 20 patients with AL (20.0%) (14 adults, 6 children) were bcr-abl(+), and in this group three cases were classified as B-acute lymphoblastic leukemia (B-ALL), and one as acute myeloblastic leukemia (AML). Two of the three patients with B-ALL were positive for b2a2 and the other one for...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Multiplex-PCR; Ph chromosome; BCR-ABL; Chronic myeloid leukemia; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400003

Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility
Provedor de dados: Genetics and Molecular Biology
Autores: Liu,Yunqiang; Tao,Dachang; Lu,Yongjie; Yang,Yuan; Ma,Yongxin; Zhang,Sizhong.
The mouse testis-enriched Znf230 gene, which encodes a type of RING finger protein, is present primarily in the nuclei of spermatogonia, the acrosome and the tail of spermatozoa. To investigate the role of Znf230 in spermatogenesis, we generated Znf230-deficient mice by disrupting Znf230 exon-5 and exon-6 using homologous recombination. The homozygous Znf230-knockout (KO) mice did not exhibit Znf230 mRNA expression and Znf230 protein production. Znf230 KO mice exhibited no obvious impairment in body growth or fertility. Male Znf230 KO mice had integral reproductive systems and mature sperm that were regular in number and shape. The developmental stages of male germ cells of Znf230 KO mice were also normal. We further examined variations in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Znf230; Knockout mice; Spermatogenesis; Kctd14.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500014

Very low mitochondrial variability in a stingless bee endemic to cerrado
Provedor de dados: Genetics and Molecular Biology
Autores: Brito,Rute Magalhães; Francisco,Flávio de Oliveira; Françoso,Elaine; Santiago,Leandro Rodrigues; Arias,Maria Cristina.
Partamona mulata is a stingless bee species endemic to cerrado, a severely threatened phytogeographical domain. Clearing for pasture without proper soil treatment in the cerrado facilitates the proliferation of termite ground nests, which are the nesting sites for P. mulata. The genetic consequences of these changes in the cerrado environment for bee populations are still understudied. In this work, we analyzed the genetic diversity of 48 colonies of P. mulata collected throughout the species' distribution range by sequencing two mitochondrial genes, cytochrome oxidase I and cytochrome B. A very low polymorphism rate was observed when compared to another Partamona species from the Atlantic forest. Exclusive haplotypes were observed in two of the five areas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado; MtDNA; Genetic variability; Partamona mulata.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100018

Chromosome numbers and meiotic behavior of some Paspalum accessions
Provedor de dados: Genetics and Molecular Biology
Autores: Adamowski,Eleniza de Victor; Pagliarini,Maria Suely; Bonato,Andréa Beatriz Mendes; Batista,Luiz Alberto Rocha; Valls,José Francisco Montenegro.
Chromosome number and meiotic behavior were evaluated in 36 Brazilian accessions of the grass Paspalum (which had never previously been analyzed) to determinate which accessions might be useful in interspecific hybridizations. The analysis showed that one accession of Paspalum coryphaeum was diploid (2n = 2x = 20) and one accession of Paspalum conspersum hexaploid (2n = 6x = 60), the remaining 34 accessions being tetraploid (2n = 4x = 40). The pairing configuration was typical for the ploidy level i.e. in the diploid, chromosomes paired as 10 bivalents, in tetraploids as bi-, tri- and quadrivalents, and in hexaploid as 30 bivalents. A low frequency of meiotic abnormalities (less than 10%) was observed in the diploid, hexaploid and some tetraploid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paspalum; Forage grass; Chromosome numbers; Meiotic behavior.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500020

Intraspecific crosses resulting in the first occurrence of eight and nine B chromosomes in Prochilodus lineatus (Characiformes, Prochilodontidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Voltolin,Tatiana Aparecida; Senhorini,José Augusto; Foresti,Fausto; Bortolozzi,Jehud; Porto-Foresti,Fábio.
B chromosomes are supernumerary elements present in about 15% of eukaryotic species and are most frequently heterochromatic, behave parasitically, show a transmission rate higher than standard (A) chromosomes, and can provoke harmful effects on carriers. In the current work, Prochilodus lineatus individuals carrying eight and nine B chromosomes were obtained by induced crossing performed involving breeders with different B chromosome numbers in their cells. The high B chromosome numbers found in the offspring were recorded for the first time in this species. The use of cytogenetic techniques applied in the present study revealed that regardless of the increase in number of B chromosomes in the genome of these individuals, those elements did not presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Supernumerary chromosome; Heterochromatic chromosome; Cross-fertilization.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200009

Phylogeographic history of South American populations of the silky anteater Cyclopes didactylus (Pilosa: Cyclopedidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Coimbra,Raphael Teodoro Franciscani; Miranda,Flávia Regina; Lara,Camila Clozato; Schetino,Marco Antônio Alves; Santos,Fabrício Rodrigues dos.
Abstract Cyclopes didactylus, commonly called silky anteater, is the smallest and least studied of the anteaters. It is an arboreal species occurring in rainforests, ranging from southern Mexico to Central and South America, with an apparently disjoint distribution between Amazon and Atlantic rainforests in Brazil. Although seven subspecies are recognized, little is known about its geographical variation. Thus, to evaluate the population dynamics and evolutionary history of the South American silky anteater, we analyzed 1542 bp sequences of the mitochondrial control region (CR), COI and Cyt-b genes of 32 individuals. Haplotype network, AMOVA and molecular dating analyses were performed and identified seven geographic clusters. The split of lineages...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xenarthra; Mitochondrial DNA; Population structure; Molecular dating; South America.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100040

Epistasis and genotype-by-environment interaction of grain protein content in durum wheat
Provedor de dados: Genetics and Molecular Biology
Autores: Bnejdi,Fethi; Gazzah,Mohamed El.
Parental, F1, F2, BC1 and BC2 generations of four crosses involving four cultivars of durum wheat (Triticum durum Desf.) were evaluated at two sites in Tunisia. A three-parameter model was found inadequate for all cases except crosses Chili x Cocorit 71 at site Sidi Thabet and Inrat 69 x Karim at both sites. In most cases a digenic epistatic model was sufficient to explain variation in generation means. Dominance effects (h) and additive x additive epistasis (i) (when significant) were more important than additive (d) effects and other epistatic components. Considering the genotype-by-environment interaction, the non-interactive model (m, d, h, e) was found adequate. Additive variance was higher than environmental variance in three crosses at both sites....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epistasis; Genotype x environment interaction; Heritability; Grain protein; Wheat.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100021

Karyotype diversity between species of Crenicichla (Perciformes, Cichlidae) from different Brazilian hydrographic basins
Provedor de dados: Genetics and Molecular Biology
Autores: Pires,Larissa Bettin; Usso,Mariana Campaner; Giuliano-Caetano,Lucia; Dias,Ana Lúcia.
Abstract Crenicichla is the largest genus in the Cichlidae family in South America. The genus includes 100 valid species that are popularly known in Brazil as jacundás or joaninhas and are widely distributed in rivers east of the Andes. Cytogenetic analyses were carried out on seven species in this genus. All species showed a diploid number of 48 with interspecific differences in karyotype formulas and AgNORs located in interstitial position on the short arm of the largest metacentric pair, except for the two populations from C. britskii. Population A showed terminal markings on the long arm of the fifth pair of the complement, and population B showed up to two marked chromosome pairs. FISH with an 18S rDNA probe was coincident with AgNORs and CMA3, except...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome banding; Fish cytogenetics; Geophaginae; Ribosomal DNA.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300357

Analysis of the genetic relationships from different genetic systems between the amylose content and the appearance quality of indica rice across environments
Provedor de dados: Genetics and Molecular Biology
Autores: Ge,Guo-Ke; Shi,Chun-Hai; Wu,Jian-Guo; Ye,Zi-Hong.
The genetic relationships between amylose content (AC) and appearance quality traits of indica rice (Oryza sativa L.) were investigated using conditional analysis and unconditional analysis in present experiment. The results from the unconditional analysis indicated that AC of rice positively correlated with brown rice (BR, i.e., dehulled but unmilled rice) length (BRL), width (BRW) and thickness (BRT), but was negatively correlated with the ratio of length to width (RLW). The conditional analysis showed that weight of brown rice (WBR) negatively affected the genetic relationships between AC and the appearance quality traits of rice except between AC and BRW, while the genetic relationships between AC and most appearance quality traits were negatively...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amylose content; Genetic relationships; Conditional and unconditional analysis; Indica rice quality traits.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400018

Characterization of different strains of common carp (Cyprinus carpio L.) (Cyprinidae, Cypriniformes) in Bangladesh using microsatellite DNA markers
Provedor de dados: Genetics and Molecular Biology
Autores: Mondol,Rashedul Kabir; Islam,Shahidul; Alam,Samsul.
Characterization of different strains of common carp (Cyprinus carpio L.) using molecular markers is essential for the management of this fish in respect to the evaluation of the potential genetic effects induced by hatchery operations and the genetic improvement of carp varieties. Five microsatellite loci (MFW1, MFW2, MFW11, MFW15 and MFW20) were analyzed for the molecular characterization of four common carp strains, i.e. scaled carp, mirror carp, red carp and koi carp. We observed differences in heterozygosities and the average numbers of alleles but not in polymorphic loci (P95) among the strains. Koi carp displayed the highest level of variability in terms of heterozygosity. The Nm values and the F ST values indicated a low level of gene flow and high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characterization; Cyprinus carpio; Microsatellite marker.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400009

Evaluation of oxidative stress markers and cardiovascular risk factors in Fabry Disease patients
Provedor de dados: Genetics and Molecular Biology
Autores: Müller,Karen B.; Galdieri,Luciano C.; Pereira,Vanessa G.; Martins,Ana M.; D'Almeida,Vânia.
Fabry Disease, an X-linked inborn error of metabolism, is characterized by progressive renal insufficiency, with cardio and cerebrovascular involvement. Homocysteine (Hcy) is considered a risk factor for vascular diseases, but the mechanisms by which it produces cardiovascular damage are still poorly understood. Regarding the vascular involvement in FD patients, the analysis of factors related to thromboembolic events could be useful to improving our understanding of the disease. The aim of this study was to evaluate plasma Hcy and other parameters involved in the methionine cycle, as well as oxidative stress markers. The sample consisted of a group of 10 male FD patients and a control group of 8 healthy individuals, paired by age. Venous blood was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inborn errors of metabolism; Fabry Disease; Homocysteine; Oxidative stress.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300006

The biology and potential for genetic research of transposable elements in filamentous fungi
Provedor de dados: Genetics and Molecular Biology
Autores: Fávaro,Léia Cecilia de Lima; Araújo,Welington Luiz de; Azevedo,João Lúcio de; Paccola-Meirelles,Luzia Doretto.
Recently many transposable elements have been identified and characterized in filamentous fungi, especially in species of agricultural, biotechnological and medical interest. Similar to the elements found in other eukaryotes, fungal transposons can be classified as class I elements (retrotransposons) that use RNA and reverse transcriptase and class II elements (DNA transposons) that use DNA. The changes (transposition and recombination) caused by transposons can supply wide-ranging genetic variation, especially for species that do not have a sexual phase. The application of transposable elements to gene isolation and population analysis is an important tool for molecular biology and studies of fungal evolution.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transposable elements; Filamentous fungi; Genetic application.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500024

Chromosome divergence and NOR polymorphism in Bryconamericus aff. iheringii (Teleostei, Characidae) in the hydrographic systems of the Paranapanema and Ivaí Rivers, Paraná, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Capistano,Thiago Gomes; Castro,Ana Luiza de Brito Portela; Julio-Junior,Horácio Ferreira.
Cytogenetic studies were carried out in three populations of Bryconamericus aff. iheringii from two hydrographic systems of the Paranapanema and Ivaí Rivers, separated by a watershed, both belonging to the upper Paraná River basin. Specimens had a constant diploid number 2n = 52 chromosomes. However, three karyotype formulae were identified in the three populations: B. aff. iheringii from the Maringá stream had 12M+18SM+8ST+14A (FN = 90); specimens from Keller River showed 8M+28SM+6ST+10A (FN = 94) and specimens from the Tatupeba stream had 8M+20SM+8ST+16A (FN = 88). Nucleolar organizer regions (NORs) were identified by silver nitrate staining and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. Specimens from Tatupeba stream had a simple...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bryconamericus; Characid fish; Chromosome divergence; Fluorescent in situ hybridization; NOR polymorphism.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200006

Genotoxicity assessment of Copaiba oil and its fractions in Swiss mice
Provedor de dados: Genetics and Molecular Biology
Autores: Almeida,Mara Ribeiro; Darin,Joana D'Arc Castania; Hernandes,Lívia Cristina; Ramos,Mônica Freiman de Souza; Antunes,Lusânia Maria Greggi; Freitas,Osvaldo de.
Copaiba oil-resin, extracted from the trunk of Copaifera, and traditionally used in folk medicine in the treatment of various disorders, has been shown to be an effective antiinflamatory, antitumor, antitetanus, antiseptic and antiblenorrhagea agent. As, there are few studies evaluating its genotoxicity, this aspect of the commercial oil-resin, and its volatile and resinous fractions, were evaluated in mice by comet assay and micronucleus (MN) test. A single dose of oil resin, volatile or resin fractions (500; 1,000 or 2,000 mg/kg b.w.) was administered by gavage. The chemical compositions of Copaiba oil resin and its fractions was analyzed by gas chromatography. According to comet assaying, treatment with either one did not increase DNA damage, and as to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copaiba oilresin; Genotoxicity; Comet assay; Micronucleus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400018

A biogeographical population genetics perspective of the colonization of cats in Latin America and temporal genetic changes in Brazilian cat populations
Provedor de dados: Genetics and Molecular Biology
Autores: Ruiz-García,Manuel; Alvarez,Diana.
We used nine morphological genes to analyze cat populations from Mexico, the Dominican Republic, the Colombian, Brazilian and Peruvian Amazon, Bolivia and Brazil. Most populations were in Hardy-Weinberg equilibrium at the O locus. The highest allele frequencies so far detected at world level for alleles I (inhibitor) and L (long hair) were found at La Paz (Bolivia). The analyses revealed at least five cat gene pools in Latin America: These findings suggest that the current genetic distribution of cats in Latin America correlates with the colonization of the Americas during the XIV to XVIII centuries. Additionally, the cat populations of São Paulo, Rio de Janeiro and Manaus were sampled, to compare their 1996-2003 genetic profiles with those obtained in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Domestic cats; Latin American cats; Population genetics; Genetic changes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400026

Lack of detection of human papillomavirus DNA in prostate carcinomas in patients from northeastern Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Araujo-Neto,Ari P.; Ferreira-Fernandes,Hygor; Amaral,Carolina M.M.; Santos,Lina G.; Freitas,Antônio C.; Silva-Neto,Jacinto C.; Rey,Juan A.; Burbano,Rommel R.; Silva,Benedito B. da; Yoshioka,France K.N.; Pinto,Giovanny R.
Abstract Prostate cancer is the second most common cancer among men in western populations, and despite its high mortality, its etiology remains unknown. Inflammatory processes are related to the etiology of various types of tumors, and prostate inflammation, in particular, has been associated with prostate cancer carcinogenesis and progression. Human papillomavirus (HPV) is associated with benign and malignant lesions in the anogenital tract of both females and males. The possible role of HPV in prostate carcinogenesis is a subject of great controversy. In this study, we aimed to examine the prevalence of HPV infections in prostate carcinomas of patients from northeastern Brazil. This study included 104 tissue samples from primary prostate carcinoma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Prostate cancer; Prevalence; HPV PCR detection; Molecular epidemiology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100024

Chromosomal characterization of Amazonian freshwater stingrays with evidence for new karyomorphs and XX/XY sex chromosomes
Provedor de dados: Genetics and Molecular Biology
Autores: Valentim,Francisco Carlos de Souza; Porto,Jorge Ivan Rebelo; Feldberg,Eliana.
Abstract Cytogenetic studies in the subfamily Potamotrygoninae have provided valuable insights into the understanding of the evolution and diversification of its species. In the present study, the chromosomal features of seven nominal potamotrygonin species are provided: Plesiotrygon iwamae (2n=74, FN=120), Potamotrygon amazona (2n=66, FN=107), P. constellata (2n=66, FN=110), P. leopoldi (2n=64, FN=102), P. motoro (2n=66, FN=106) from four different localities, and P. orbignyi (2n=66, FN=106), P. scobina (2n=66, FN=104), from Central Amazon. Additionally, we found a new karyomorph in P. wallacei. We considered the localization of Nucleolus Organizer Regions (NORs), as well as the pattern of constitutive heterochromatin, as species-specific characters. We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elasmobranchs; Cytogenetics; Chromosomal rearrangements; Sex chromosomes; Karyotypic diversity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400578

Mitochondrial tRNA gene translocations in highly eusocial bees
Provedor de dados: Genetics and Molecular Biology
Autores: Silvestre,Daniela; Arias,Maria Cristina.
Mitochondrial gene rearrangement events, especially involving tRNA genes, have been described more frequently as more complete mitochondrial genome sequences are becoming available. In the present work, we analyzed mitochondrial tRNA gene rearrangements between two bee species belonging to the tribes Apini and Meliponini within the "corbiculate Apidae". Eleven tRNA genes are in different genome positions or strands. The molecular events responsible for each translocation are explained. Considering the high number of rearrangements observed, the data presented here contradict the general rule of high gene order conservation among closely related organisms, and also represent a powerful molecular tool to help solve questions about phylogeny and evolution in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; Melipona bicolor; Mitogenomics; Gene order; Eusociality.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300030

Association of GH and IGF-1 polymorphisms with growth traits in a synthetic beef cattle breed
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Andréa Pozzi; Alencar,Maurício Mello de; Oliveira,Henrique Nunes de; Regitano,Luciana Correia de Almeida.
The Canchim beef cattle (5/8 Charolais + 3/8 Zebu) has been selected for meat production in Brazil since late 1950. In the present work the effects of growth hormone (GH) and insulin-like growth factor 1 (IGF-1) polymorphisms were investigated in 688 animals born between 1998 and 2000. These animals belonged to two genetic groups, i.e., traditional and new lineages. Genotype effects on expected breeding values for birth weight (BW), weaning weight (WW) and yearling weight (YW) were investigated by the least square method. Significant effects were found for GH genotype on YW (p &lt; 0.05), with positive effects associated with the LV (leucine/valine) genotype. For IGF-1 genotypes, significant effects were found on BW (p &lt; 0.01) and YW (p &lt; 0.01)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Genetic association; Genotype; Growth hormone; Insulin-like growth factor 1.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200009

Mapping of dentin-specific acidic phosphoprotein and integrin-binding sialoprotein in sheep defines an inversion breakpoint with respect to human chromosome 4Q
Provedor de dados: Genetics and Molecular Biology
Autores: Lumsden,Joanne M.; Lord,Eric A.; Hirst,Karen L.; Dixon,Michael J.; Montgomery,Grant W.
Genes from sheep chromosome 6 map to human chromosome 4 in the region extending from 4p16 to 4q26. However, there is an inversion of gene order in the central portion of the chromosome with one breakpoint close to secreted phosphoprotein 1 (SPP1). Genes for SPP1, integrin-binding sialoprotein (IBSP) and dentin-specific acidic phosphoprotein (DMP1) are located close together in a YAC contig in the human. RFLP markers were developed for DMP1 and IBSP in sheep and located on the sheep linkage map to further define the breakpoint region. There were no recombinants between SPP1 and IBSP indicating that these loci are close together in sheep, as in humans. DMP1 was located approximately 80 cM from SPP1 in sheep, 7 cM from the microsatellite BMC4203. In the human...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100007

Cytological and genome size data analyzed in a phylogenetic frame: Evolutionary implications concerning Sisyrinchium taxa (Iridaceae: Iridoideae)
Provedor de dados: Genetics and Molecular Biology
Autores: Burchardt,Paula; Souza-Chies,Tatiana T.; Chauveau,Olivier; Callegari-Jacques,Sidia M.; Brisolara-Corrêa,Lauís; Inácio,Camila Dellanhese; Eggers,Lilian; Siljak-Yakovlev,Sonja; de Campos,José Marcello Salabert; Kaltchuk-Santos,Eliane.
Abstract Sisyrinchium is the largest genus of Iridaceae in the Americas and has the greatest amount of cytological data available. This study aimed at investigating how genomes evolved in this genus. Chromosome number, genome size and altitude from species of sect. Viperella were analyzed in a phylogenetic context. Meiotic and pollen analyses were performed to assess reproductive success of natural populations, especially from those polyploid taxa. Character optimizations revealed that the common ancestor of sect. Viperella was probably diploid (2n = 2x =18) with two subsequent polyplodization events. Total DNA content (2C) varied considerably across the phylogeny with larger genomes detected mainly in polyploid species. Altitude also varied across the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ancestral chromosome number; DNA content; Meiotic behavior; Pollen viability; Polyploidy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200288

Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves)
Provedor de dados: Genetics and Molecular Biology
Autores: Faria,Patrícia J.; Miyaki,Cristina Y.
The selection of molecular markers for population studies is an important tool for biodiversity conservation. The family Psittacidae contains many endangered and vulnerable species and we tested three kinds of molecular markers for their potential use in population studies of five psitacid species: 43 hyacinth macaws (Anodorhynchus hyacinthinus), 42 blue-and-yellow macaws (Ara ararauna), 23 red-and-green macaws (Ara chloroptera), 19 red-spectacled amazons (Amazona pretrei); and 18 red-tailed amazons (Amazona brasiliensis). We tested 21 clones from a genomic library of golden conure (Guarouba guarouba) minisatellites and 12 pairs of microsatellite primers developed for the domestic chicken (Gallus gallus) and A. hyacinthinus. We also tested seven...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Minisatellites; Molecular markers; Parrots; Population studies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200007

Genotype by environment interaction in Nelore cattle from five Brazilian states
Provedor de dados: Genetics and Molecular Biology
Autores: Diaz,Iara Del Pilar Solar; Oliveira,Henrique Nunes de; Bezerra,Luis Antônio Framartino; Lôbo,Raysildo Barbosa.
Records from 75,941 Nelore cattle were used to determine the importance of genotype by environment interaction (GEI) in five Brazilian states. (Co)variance components were estimated by single-trait analysis (with yearling weight, W450, considered to be the same trait in all states) and multiple-trait analysis (with the record from each state considered to be a different trait). The direct heritability estimates for yearling weight were 0.51, 0.39, 0.44, 0.37 and 0.41 in the states of Goiás, Mato Grosso, São Paulo, Mato Grosso do Sul and Minas Gerais, respectively. The across-state genetic correlation estimates between Goiás and Mato Grosso, Goiás and Minas Gerais, São Paulo and Minas Gerais, and Mato Grosso do Sul and Minas Gerais ranged from 0.67 to 0.75....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bayesian inference; Beef cattle; Genetic correlation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300012

Genetic diversity of carotenoid-rich bananas evaluated by Diversity Arrays Technology (DArT)
Provedor de dados: Genetics and Molecular Biology
Autores: Amorim,Edson P.; Vilarinhos,Alberto D.; Cohen,Kelly O.; Amorim,Vanusia B.O.; Santos-Serejo,Janay A. dos; Silva,Sebastião Oliveira e; Pestana,Kátia N.; Santos,Vânia J. dos; Paes,Norma S.; Monte,Damares C.; Reis,Ronaldo V. dos.
The aim of this work was to evaluate the carotenoid content and genetic variability of banana accessions from the Musa germplasm collection held at Embrapa Cassava and Tropical Fruits, Brazil. Forty-two samples were analyzed, including 21 diploids, 19 triploids and two tetraploids. The carotenoid content was analyzed spectrophotometrically and genetic variability was estimated using 653 DArT markers. The average carotenoid content was 4.73 µg.g-1, and ranged from 1.06 µg.g-1 for the triploid Nanica (Cavendish group) to 19.24 µg.g-1 for the triploid Saney. The diploids Modok Gier and NBA-14 and the triploid Saney had a carotenoid content that was, respectively, 7-fold, 6-fold and 9-fold greater than that of cultivars from the Cavendish group (2.19 µg.g-1)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diploids; Germplasm; Molecular markers; Musa sp.; Variability.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100014

Chromosomal polymorphism in 12 populations of Mikania micrantha (Compositae)
Provedor de dados: Genetics and Molecular Biology
Autores: Maffei,Eliane M.D.; Marin-Morales,M.A.; Ruas,P.M.; Ruas,C.F.; Matzenbacher,N.I.
Mikania micrantha is a climbing perennial weed of the family Asteraceae, with a vast distribution from South America to south of the United States. This species is widely distributed throughout Brazil, where it shows little morphological variation. Mitotic chromosomes of 12 populations of M. micrantha derived from several Brazilian sites were studied using Feulgen staining and C-banding. The populations included eight diploid (2n = 36 and 42) and four tetraploid (2n = 72) cytotypes. Chromosome numbers of 2n = 36 and 2n = 42 are reported for the first time for M. micrantha. These populations had a secondary constriction in the middle of the larger arm of chromosome pair 1, following the same pattern described for all Mikania species analyzed so far....
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300025

Chromosome 19p13.3 deletion in a child with Peutz-Jeghers syndrome, congenital heart defect, high myopia, learning difficulties and dysmorphic features: clinical and molecular characterization of a new contiguous gene syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Josiane; Faucz,Fábio; Sotomaior,Vanessa; Bonalumi Filho,Aguinaldo; Rosenfeld,Jill; Raskin,Salmo.
The Peutz-Jeghers syndrome (PJS) is an autosomal-dominant hamartomatous polyposis syndrome characterized by mucocutaneous pigmentation, gastrointestinal polyps and the increased risk of multiple cancers. The causative point mutation in the STK11 gene of most patients accounts for about 30% of the cases of partial and complete gene deletion. This is a report on a girl with PJS features, learning difficulties, dysmorphic features and cardiac malformation, bearing a de novo 1.1 Mb deletion at 19p13.3. This deletion encompasses at least 47 genes, including STK11. This is the first report on 19p13.3 deletion associated with a PJS phenotype, as well as other atypical manifestations, thereby implying a new contiguous gene syndrome.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 19p13.3 deletion; Comparative genomic hybridization array; Contiguous gene syndrome; Peutz-Jeghers syndrome; STK11 gene.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400005

Analysis of mRNA transcripts in chronic myeloid leukemia patients
Provedor de dados: Genetics and Molecular Biology
Autores: Meissner,Rosely de V.; Covas,Dimas T.; Dias,Paula M.B.; Job,Fani; Leite,Márcia; Nardi,Nance B.
The nature of BCR/ABL hybrid mRNA was analyzed by RT-PCR in cells from 33 patients (22 males, 11 females) with chronic myeloid leukemia (CML). b3a2 mRNA was found in 14 cases, whereas 13 patients had b2a2 mRNA and six had both kinds of mRNA, with a predominance of the b3a2 type. The type of mRNA present showed no significant correlation with age, hemoglobin level, number of leukocytes and platelets, percentage of blasts or basophils or the presence of splenomegaly at diagnosis. There was also no correlation with sex or duration of the chronic phase. When these results were combined with those reported by other groups, a significant association (P = 0.029) was observed for mRNA type vs. sex, with a predominance of men in the groups expressing b2a2 (2.68:1)...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400003

Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi
Provedor de dados: Genetics and Molecular Biology
Autores: Torres-Silva,Carolina Furtado; Repolês,Bruno Marçal; Ornelas,Hugo Oliveira; Macedo,Andréa Mara; Franco,Glória Regina; Junho Pena,Sérgio Danilo; Tahara,Erich Birelli; Machado,Carlos Renato.
Abstract Trypanosoma cruzi is the etiological agent of Chagas disease, a public health challenge due to its morbidity and mortality rates, which affects around 6-7 million people worldwide. Symptoms, response to chemotherapy, and the course of Chagas disease are greatly influenced by T. cruzi‘s intra-specific variability. Thus, DNA mutations in this parasite possibly play a key role in the wide range of clinical manifestations and in drug sensitivity. Indeed, the environmental conditions of oxidative stress faced by T. cruzi during its life cycle can generate genetic mutations. However, the lack of an established experimental design to assess mutation rates in T. cruzi precludes the study of conditions and mechanisms that potentially produce genomic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: T. cruzi; DNA; Mutation frequency; H2O2.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300466

Karyotype differentiation in three species of Tripogandra Raf. (Commelinaceae) with different ploidy levels
Provedor de dados: Genetics and Molecular Biology
Autores: Marques,André; Roa,Fernando; Guerra,Marcelo.
Most species of the genus Tripogandra (Commelinaceae) are taxonomically poorly circumscribed, in spite of having a relatively stable basic number x = 8. Aiming to estimate the cytological variation among Tripogandra species carrying this base number, several structural karyotypic characters were investigated in the diploid T. glandulosa, the hexaploid T. serrulata, and the octoploid T. diuretica. A careful evaluation of chromosome size and morphology did not reveal clear chromosome homeologies among karyotypes. The mean chromosome size was strongly reduced in the octoploid species, but not in the hexaploid species. They also differed largely in the CMA+ banding pattern and in the number of 5S and 45S rDNA sites per monoploid chromosome complement. All...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tripogandra; Cytotaxonomy; RDNA sites; CMA/DAPI bands; Meiosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400020

Use of signal thresholds to determine significant changes in microarray data analyses
Provedor de dados: Genetics and Molecular Biology
Autores: Xinmin,Li; Kim,Jaejung; Zhou,Jian; Gu,Weikuan; Quigg,Richard.
The use of a constant fold-change to determine significant changes in gene expression has been widely accepted for its intuition and ease of use in microarray data analysis, but this concept has been increasingly criticized because it does not reflect signal intensity and can result in a substantial number of false positives and false negatives. To resolve this dilemma, we have analyzed 65 replicate Affymetrix chip-chip comparisons and determined a series of user adjustable signal-dependent thresholds which do not require replicates and offer a 95% confidence interval. Quantitative RT-PCR shows that such thresholds significantly improve the power to discriminate biological changes in mRNA from noise and reduce false calls compared to the traditional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microarray; Signal threshold; Affymetrix; Data analysis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200002

Genome-wide identification, classification and transcriptional analysis of nitrate and ammonium transporters in Coffea
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Tiago Benedito dos; Lima,Joni Esrom; Felicio,Mariane Silva; Soares,João Danillo Moura; Domingues,Douglas Silva.
Abstract Nitrogen (N) is quantitatively the main nutrient required by coffee plants, with acquisition mainly by the roots and mostly exported to coffee beans. Nitrate (NO3–) and ammonium (NH4+) are the most important inorganic sources for N uptake. Several N transporters encoded by different gene families mediate the uptake of these compounds. They have an important role in source preference for N uptake in the root system. In this study, we performed a genome-wide analysis, including in silico expression and phylogenetic analyses of AMT1, AMT2, NRT1/PTR, and NRT2 transporters in the recently sequenced Coffea canephora genome. We analyzed the expression of six selected transporters in Coffea arabica roots submitted to N deficiency. N source preference was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Nitrogen transport; Nitrogen uptake; Gene family evolution.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200346

Smith-Lemli-Opitz syndrome: clinical and biochemical findings in Brazilian patients. Scalco et al. Genetics and Molecular Biology (this issue)
Provedor de dados: Genetics and Molecular Biology
Autores: Opitz,John M.
Tipo: Info:eu-repo/semantics/article
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300004

Genetic diversity and geographical differentiation of cultivated six-rowed naked barley landraces from the Qinghai-Tibet plateau of China detected by SSR analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Feng,Zong-Yun; Zhang,Li-Li; Zhang,Yi-Zheng; Ling,Hong-Qing.
Cultivated six-rowed naked barley (Hordeum vulgare ssp. hexastichon var. nudum Hsü) is the oldest cultivated barley in China. We used 35 simple sequence repeat (SSR) markers selected from seven barley linkage groups to study the genetic diversity, geographical differentiation and evolutionary relationships among 65 H. vulgare ssp. hexastichon landrace accessions collected from the Qinghai-Tibet plateau of China, 25 accessions from Tibet (TB), 20 from Qinghai (QH) and 20 from Ganzi (GZ) prefecture in Sichuan province. At the 35 SSR loci we identified 248 alleles among the 65 accessions, 119 (47.98%) of the alleles being common alleles. We also found that the TB accessions possessed 47 private alleles, about 1.5 times more than the 31 private alleles found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Barley; Genetic diversity; Qinghai-Tibet plateau; Geographical differentiation; Simple sequence repeat marker.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200022

Species diversity and geographic distribution of Gymnotus (Pisces: Gymnotiformes) by nuclear (GGAC)n microsatellite analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Fernandes-Matioli,F.M.C.; Matioli,S.R.; Almeida-Toledo,L.F.
Patterns of amplified DNA fragments flanked by (GGAC)n microsatellites, obtained by single primer amplification reaction (SPAR), from 198 Gymnotus specimens (Pisces: Gymnotiformes) sampled from 8 southeastern Brazilian river basins were analyzed. The species studied were Gymnotus carapo, G. pantherinus, G. inaequilabiatus, and G. sylvius. The indirectly obtained patterns reflected the distribution of simple sequence repeats in the nuclear genome of the specimens. Species-specific patterns of DNA amplification were found and were useful for the analysis of the geographic distribution of Gymnotus species. Monomorphic patterns were found in G. carapo, G. pantherinus, and G. inaequilabiatus. Three polymorphic patterns were found in G. sylvius populations. The...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400016

Editorial
Provedor de dados: Genetics and Molecular Biology
Tipo: Info:eu-repo/semantics/other
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200001