Consulta: creatorFacets:"Godfroy, Anne"
Registros recuperados: 42
Data/hora: 09/06/2024 03:38:15

Desulfurobacterium crinifex sp.nov., a novel thermophilic, pinkish-streamer forming, chemolithoautotrophic bacterium isolated from a Juan de Fuca Ridge hydrothermal vent and amendment of the genus Desulfurobacterium
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Alain, Karine; Rolland, Sophie; Crassous, Philippe; Lesongeur, Francoise; Zbinden, Magali; Le Gall, Christian; Godfroy, Anne; Page, Antoine; Juniper, S Kim; Cambon-bonavita, Marie-anne; Duchiron, Francis; Querellou, Joel.
A novel thermophilic, chemolithoautotrophic T bacterium, designated as NE1206(T), was isolated from a Juan de Fuca Ridge hydrothermal vent sample (tubes of the annelid polychaete Paralvinella sulfincola attached to small pieces of hydrothermal chimney). The cells were rod-shaped (1.2-3.5x0.4-0.7 mum), occurring as single motile rods or forming macroscopic aggregates visible as pinkish to brownish streamers. The new isolate was anaerobic. It grew between 50 and 70 degreesC (optimum 60-65 degreesC doubling time approximately 1 h 15 min at 60 degreesC), between pH 5.0 and 7.5 (optimum pH around 6.0-6.5) and at sea salts concentrations between 20 and 40 g l(-1) (optimum 30 g l(-1)). Cells grew chemolithoautotrophically in an H-2/CO2 atmosphere (80/20, v/v; 200...
Tipo: Text
Palavras-chave: Thermophile; Nitrate reducing; Lithotroph; Desulfurobacterium crinifex; Deep sea hydrothermal vent; Cell networks.
Ano: 2003
URL: https://archimer.ifremer.fr/doc/2003/publication-663.pdf

Sedimentological imprint on subseafloor microbial communities in Western Mediterranean Sea Quaternary sediments
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ciobanu, Maria Cristina; Rabineau, Marina; Droz, Laurence; Revillon, Sidonie; Ghiglione, J. -f.; Dennielou, Bernard; Jorry, Stephan; Kallmeyer, J.; Etoubleau, Joel; Pignet, Patricia; Crassous, Philippe; Vandenabeele-trambouze, Odile; Laugier, J.; Guegan, Matthieu; Godfroy, Anne; Alain, Karine.
An interdisciplinary study was conducted to evaluate the relationship between geological and paleoenvironmental parameters and the bacterial and archaeal community structure of two contrasting subseafloor sites in the Western Mediterranean Sea (Ligurian Sea and Gulf of Lion). Both depositional environments in this area are well-documented from paleoclimatic and paleooceanographic point of views. Available data sets allowed us to calibrate the investigated cores with reference and dated cores previously collected in the same area, and notably correlated to Quaternary climate variations. DNA-based fingerprints showed that the archaeal diversity was composed by one group, Miscellaneous Crenarchaeotic Group (MCG), within the Gulf of Lion sediments and of nine...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00090/20171/17826.pdf

Ecophysiological differences between vesicomyid species and metabolic capabilities of their symbionts influence distribution patterns of the deep‐sea clams
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cruaud, Perrine; Decker, Carole; Olu, Karine; Arnaud-haond, Sophie; Papot, Claire; Le Baut, Jocelyn; Vigneron, Adrien; Khripounoff, Alexis; Gayet, Nicolas; Cathalot, Cecile; Caprais, Jean-claude; Pignet, Patricia; Godfroy, Anne; Cambon Bonavita, Marie-anne.
This study provides an analysis of vesicomyid bivalve–symbiont community distribution across cold seep and hydrothermal vent areas in the Guaymas Basin (Gulf of California, Mexico). Using a combination of morphological and molecular approaches including fluorescent in situ hybridization (FISH), and electronic microscopy observations, vesicomyid clam species and their associated symbionts were characterized and results were analyzed in light of geochemical conditions and other on‐site observations. A greater diversity of vesicomyids was found at cold seep areas, where three different species were present (Phreagena soyoae [syn. kilmeri], Archivesica gigas, and Calyptogena pacifica). In contrast, A. gigas was the only species sampled across the hydrothermal...
Tipo: Text
Palavras-chave: Deep-sea ecosystems; Guaymas Basin; Marine ecology; Pliocardinae bivalve; Sulfur storage; Vesicomyid movements.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00493/60426/64028.pdf

Relationship between the occurrence of filamentous bacteria on Bathymodiolus azoricus shell and the physiological and toxicological status of the vent mussel
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Martins, I.; Colaco, A.; Santos, Raphael; Lesongeur, Francoise; Godfroy, Anne; Sarradin, Pierre-marie; Cosson, R. P.
The edifice walls of the Eiffel Tower hydrothermal vent site (Mid-Atlantic Ridge, Lucky Strike vent field) are populated with dense communities of dual symbioses harboring vent mussel Bathymodiolus azoricus, some of which are covered by white filamentous mats belonging to sulfur-oxidizing bacteria. Mussels were collected in both the presence and absence of the filamentous bacteria. A sample of the filamentous bacteria was collected and water measurements of temperature, CH4 and H2S were recorded at the collection area. The whole soft tissues were analyzed for total lipid, carbohydrate and total protein. Metallothioneins and metals (Cu, Fe and Zn) levels were determined in the major organs. The results showed no significant physiological and toxicological...
Tipo: Text
Palavras-chave: Vent mussel; Metals; Metallothioneins; Filamentous bacteria; Eiffel Tower; Biomarkers.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6821.pdf

Presence and diversity of anammox bacteria in cold hydrocarbon-rich seeps and hydrothermal vent sediments of the Guaymas Basin
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Russ, Lina; Kartal, Boran; Op Den Camp, Huub J. M.; Sollai, Martina; Le Bruchec, Julie; Caprais, Jean-claude; Godfroy, Anne; Damste, Jaap S. Sinninghe; Jetten, Mike S. M.
Hydrothermally active sediments are highly productive, chemosynthetic areas which are characterized by the rapid turnover of particulate organic matter under extreme conditions in which ammonia is liberated. These systems might be suitable habitats for anaerobic ammonium oxidizing (anammox) bacteria but this has not been investigated in detail. Here we report the diversity and abundance of anammox bacteria in sediments that seep cold hydrocarbon-rich fluids and hydrothermal vent areas of the Guaymas Basin in the Cortes Sea using the unique functional anammox marker gene, hydrazine synthase (hzsA). All clones retrieved were closely associated to the "Candidatus Scalindua" genus. Phylogenetic analysis revealed two distinct clusters of hzsA sequences (Ca....
Tipo: Text
Palavras-chave: Anammox; HzsA; AprA; Hydrothermal vents; Cold seep; Sulfide.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00180/29156/27555.pdf

Structural Iron (II) of Basaltic Glass as an Energy Source for Zetaproteobacteria in an Abyssal Plain Environment, Off the Mid Atlantic Ridge
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Henri, Pauline A.; Rommevaux-jestin, Celine; Lesongeur, Francoise; Mumford, Adam; Emerson, David; Godfroy, Anne; Menez, Benedicte.
To explore the capability of basaltic glass to support the growth of chemosynthetic microorganisms, complementary in situ and in vitro colonization experiments were performed. Microbial colonizers containing synthetic tholeitic basaltic glasses, either enriched in reduced or oxidized iron, were deployed off-axis from the Mid Atlantic Ridge on surface sediments of the abyssal plain (35°N; 29°W). In situ microbial colonization was assessed by sequencing of the 16S rRNA gene and basaltic glass alteration was characterized using Scanning Electron Microscopy, micro-X-ray Absorption Near Edge Structure at the Fe-K-edge and Raman microspectroscopy. The colonized surface of the reduced basaltic glass was covered by a rind of alteration made of iron-oxides trapped...
Tipo: Text
Palavras-chave: Basaltic glass; Zetaproteobacteria; Bio-mediated alteration; Iron-oxidation; Abyssal plain.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00378/48894/49332.pdf

Presence and activity of anaerobic ammonium-oxidizing bacteria at deep-sea hydrothermal vents
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Byrne, Nathalie; Strous, Marc; Crepeau, Valentin; Kartal, Boran; Birrien, Jean-louis; Schmid, Markus; Lesongeur, Francoise; Schouten, Stefan; Jaeschke, Andrea; Jetten, Mike; Prieur, Daniel; Godfroy, Anne.
Recent studies indicate that ammonia is an important electron donor for the oxidation of fixed nitrogen, both in the marine water column and sediments. This process, known as anammox, has so far only been observed in a large range of temperature habitats. The present study investigated the role of anammox in hydrothermal settings. During three oceanographic expeditions to the Mid-Atlantic Ridge, hydrothermal samples were collected from five vent sites, at depths ranging from 750 to 3650m from cold to hot habitats. Evidence for the occurrence of anammox in these particular habitats was demonstrated by concurrent surveys, including the amplification of 16S rRNA gene sequences related to known anammox bacteria, ladderanes lipids analysis and measurement of a...
Tipo: Text
Palavras-chave: Hydrothermal vent; Ladderanes; 16S rRNA; Activity; Micro organisms; Anammox.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6921.pdf

Relative abundance and diversity of bacterial methanotrophs at the oxic-anoxic interface of the Congo deep-sea fan
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bessette, Sandrine; Moalic, Yann; Gautey, Sebastien; Lesongeur, Francoise; Godfroy, Anne; Toffin, Laurent.
Sitting at ~5 000 m water depth on the Congo-Angola margin and ~ 760 km offshore of the West African coast, the recent lobe complex of the Congo deep-sea fan receives large amounts of fluvial sediments (3-5% organic carbon). This organic-rich sedimentation area harbors habitats with chemosynthetic communities similar to those of cold seeps. In this study, we investigated relative abundance, diversity and distribution of aerobic methane-oxidizing bacteria (MOB) communities at the oxic-anoxic interface of sedimentary habitats by using fluorescence in situ hybridization and comparative sequence analysis of particulate mono-oxygenase (pmoA) genes. Our findings revealed that sedimentary habitats of the recent lobe complex hosted type I and type II MOB cells and...
Tipo: Text
Palavras-chave: Aerobic methane-oxidizing bacteria; PmoA; Organic-rich sediment; Methane seeps; Congo deep-sea fan.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00382/49327/49706.pdf

The Congolobe project, a multidisciplinary study of Congo deep-sea fan lobe complex: Overview of methods, strategies, observations and sampling
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rabouille, C.; Olu, Karine; Baudin, F.; Khripounoff, Alexis; Dennielou, Bernard; Arnaud-haond, Sophie; Babonneau, Nathalie; Bayle, Christophe; Beckler, J.; Bessette, Sandrine; Bombled, B.; Bourgeois, S.; Brandily, Christophe; Caprais, Jean-claude; Cathalot, Cecile; Charlier, K.; Corvaisier, R.; Croguennec, Chantal; Cruaud, Perrine; Decker, Carole; Droz, L.; Gayet, Nicolas; Godfroy, Anne; Hourdez, S.; Le Bruchec, J.; Saout, Johan; Le Saout, Marie-helene; Lesongeur, Francoise; Martinez, P.; Mejanelle, L.; Michalopoulos, P.; Mouchel, Olivier; Noel, Philippe; Pastor, Lucie; Picot, M.; Pignet, Patricia; Pozzato, L.; Pruski, A. M.; Rabiller, Manuella; Raimonet, M.; Ragueneau, O.; Reyss, J. L.; Rodier, Philippe; Ruesch, Blandine; Ruffine, Livio; Savignac, F.; Senyarich, C.; Schnyder, J.; Sen, Arunima; Stetten, E.; Sun, Ming Yi; Taillefert, M.; Teixeira, S.; Tisnerat-laborde, N.; Toffin, Laurent; Tourolle, Julie; Toussaint, F.; Vetion, G.; Jouanneau, J. M.; Bez, M.
The presently active region of the Congo deep-sea fan (around 330 000 km2), called the terminal lobes or lobe complex, covers an area of 2500 km2 at 4700–5100 m water depth and 750–800 km offshore. It is a unique sedimentary area in the world ocean fed by a submarine canyon and a channel-levee system which presently deliver large amounts of organic carbon originating from the Congo River by turbidity currents. This particularity is due to the deep incision of the shelf by the Congo canyon, up to 30 km into the estuary, which funnels the Congo River sediments into the deep-sea. The connection between the river and the canyon is unique for major world rivers.  In 2011, two cruises (WACS leg 2 and Congolobe) were conducted to simultaneously investigate the...
Tipo: Text
Palavras-chave: Chemosynthetic habitats; Congo deep-sea fan; Fine sediment; Sedimentation rate; Seafloor morphology; Turbidite; Vesicomyidae.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00335/44580/44298.pdf

Acides organiques produits par une archaébactérie anaérobie hyperthermophile soufre-dépendante
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Guezennec, Jean; Rimbault, A; Godfroy, Anne; Fromage, M; Marec, F; Rocchiocioli, F.
The existence of deep-sea hydrothermal thermophilic archaebacteria raises interesting questions about their metabolism and metabolic products both in a natural environment and in a culture medium. Moreover, the use of these microorganisms as a potential source of molecules of biotechnological interest must be considered, in the light of the fact that metabolic produits can enhance the risk of microbially-induced corrosion. Organic acids formed by an anaerobic thermophilic sulphur-dependent archaebacterium were analysed as tert-butyldimethylsilyl derivatives by GC and GC/MS. Data indicated the presence in significant amounts of propanoic, 2-methyl propanoic, 3-(methylthio) propanoic, phenylacetic and 4-hydroxyphenylacetic acids. In addition, the use of...
Tipo: Text
Palavras-chave: ARCHAEA; ORGANIC ACIDS; AMINO ACIDS; LABELED ISOTOPES.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00099/21011/18637.pdf

Phylogenetic and Functional Diversity of Microbial Communities Associated with Subsurface Sediments of the Sonora Margin, Guaymas Basin
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Vigneron, Adrien; Cruaud, Perrine; Roussel, Erwan; Pignet, Patricia; Caprais, Jean-claude; Callac, Nolwenn; Ciobanu, Maria Cristina; Godfroy, Anne; Cragg, Barry A.; Parkes, John R.; Van Nostrand, Joy D.; He, Zhili; Zhou, Jizhong; Toffin, Laurent.
Subsurface sediments of the Sonora Margin (Guaymas Basin), located in proximity of active cold seep sites were explored. The taxonomic and functional diversity of bacterial and archaeal communities were investigated from 1 to 10 meters below the seafloor. Microbial community structure and abundance and distribution of dominant populations were assessed using complementary molecular approaches (Ribosomal Intergenic Spacer Analysis, 16S rRNA libraries and quantitative PCR with an extensive primers set) and correlated to comprehensive geochemical data. Moreover the metabolic potentials and functional traits of the microbial community were also identified using the GeoChip functional gene microarray and metabolic rates. The active microbial community structure...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00203/31408/29801.pdf

Compte-rendu opérationnel de la campagne MoMARSAT 2020 - LuckyDivMic2020
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Sarradin, Pierre-marie; Legrand, Julien; Godfroy, Anne; Cannat, Mathilde.
Ce document présente les opérations menées et recense les prélèvements effectués durant les campagnes MoMARSAT 2020 et LuckyDivMic20
Tipo: Text
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00683/79497/82109.pdf

Prokaryote Communities at Active Chimney and In‐Situ Colonization Devices after a Magmatic Degassing Event (37°N MAR, EMSO‐Azores Deep‐Sea Observatory)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rommevaux, Céline; Henri, Pauline; Degboe, Jefferson; Chavagnac, Valérie; Lesongeur, Francoise; Godfroy, Anne; Boulart, Cedric; Destrigneville, Christine; Castillo, Alain.
The mixing zone between high temperature hydrothermal fluids and seawater produces redox gradients, promoting the development of unique ecosystems based on chemotrophy. The structure of microbial communities depends on their environment, which can vary according to space and time. Hydrothermal circulation within the oceanic crust determines the chemical composition and flow of fluids, depending on underground events (earthquakes, volcanic episodes....), and impacts the development of microbial communities. This link between hydrothermal vent communities and deep geological events is the focus of the present study, the first of its kind for slow‐spreading ridge. In this study, we present a unique set of multidisciplinary data collected from 2008 to 2011 on...
Tipo: Text
Palavras-chave: Lucky Strike; Magmatic degassing; Microbial community structure; Multidisciplinary study.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00500/61174/64657.pdf

Marinitoga hydrogenitolerans sp nov., a novel member of the order Thermotogales isolated from a black smoker chimney on the Mid-Atlantic Ridge
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Postec, Anne; Le Breton, Claire; Fardeau, Marie-laure; Lesongeur, Francoise; Pignet, Patricia; Querellou, Joel; Ollivier, Bernard; Godfroy, Anne.
A novel, thermophilic, anaerobic bacterium that is able to tolerate hydrogen was isolated from a deep-sea hydrothermal chimney collected at the Rainbow field on the Mid-Atlantic Ridge. Cells were rod-shaped and surrounded by a sheath-like outer structure (toga); they were weakly motile by means of a polar flagellum. They appeared singly, in pairs or in short chains. They grew at 35-65 degrees C (optimum 60 degrees C), pH 4.5-8.5 (optimum pH 6.0) and 10-65 g sea salts l(-1) (optimum 30-40 g l(-1)). The isolate was organotrophic, and able to grow on various carbohydrates or complex proteinaceous substrates. Growth was not inhibited under 100% hydrogen or in the presence of 2% oxygen in the gas phase. The isolate reduces sulfur, although sulfur reduction is...
Tipo: Text
Palavras-chave: Anaerobic bacterium; Marinitoga hydrogenitolerans; Thermotogales; Thermophile; Deep sea vent.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-363.pdf

Crassaminicella profunda gen. nov., sp nov., an anaerobic marine bacterium isolated from deep-sea sediments
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lakhal, Raja; Pradel, Nathalie; Postec, Anne; Ivier, Bernard; Cayol, Jean-luc; Godfroy, Anne; Fardeau, Marie-laure; Gales, Gregoire.
A novel, anaerobic, chemo-organotrophic bacterium, designated strain Ra1766HT, was isolated from sediments of the Guaymas basin (Gulf of California, Mexico) taken from a depth of 2002 m. Cells were thin, motile, Gram-stain-positive, flexible rods forming terminal endospores. Strain Ra1766H(T) grew at temperatures of 25-45 degrees C (optimum 30 degrees C), pH 6.7-8.1 (optimum 7.5) and in a salinity of 5-60 g l(-1) NaCl (optimum 30 g l(-1)). It was an obligate heterotrophic bacterium fermenting carbohydrates (glucose and mannose) and organic acids (pyruvate and succinate). Casamino acids and amino acids (glutamate, aspartate and glycine) were also fermented. The main end products from glucose fermentation were acetate, butyrate, ethanol, H-2 and CO2....
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00296/40742/39992.pdf

The interplay at the replisome mitigates the impact of oxidative damage on the genetic integrity of hyperthermophilic Archaea
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Killelea, Tom; Palud, Adeline; Akcha, Farida; Lemor, Mélanie; L'Haridon, Stephane; Godfroy, Anne; Henneke, Ghislaine.
8-oxodeoxyguanosine (8-oxodG), a major oxidised base modification, has been investigated to study its impact on DNA replication in hyperthermophilic Archaea. Here we show that 8-oxodG is formed in the genome of growing cells, with elevated levels following exposure to oxidative stress. Functional characterisation of cell-free extracts and the DNA polymerisation enzymes, PolB, PolD, and the p41/p46 complex, alone or in the presence of accessory factors (PCNA and RPA) indicates that translesion synthesis occurs under replicative conditions. One of the major polymerisation effects was stalling, but each of the individual proteins could insert and extend past 8-oxodG with differing efficiencies. The introduction of RPA and PCNA influenced PolB and PolD in...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00502/61372/64989.pdf

Microbial colonization of basaltic glasses in hydrothermal organic-rich sediments at Guaymas Basin
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Callac, Nolwenn; Rommevaux-jestin, Celine; Rouxel, Olivier; Lesongeur, Francoise; Liorzou, Celine; Bollinger, Claire; Ferrant, Anthony; Godfroy, Anne.
Oceanic basalts host diverse microbial communities with various metabolisms involved in C, N, S, and Fe biogeochemical cycles which may contribute to mineral and glass alteration processes at, and below the seafloor. In order to study the microbial colonization on basaltic glasses and their potential biotic/abiotic weathering products, two colonization modules called AISICS ("Autonomous in situ Instrumented Colonization System") were deployed in hydrothermal deep-sea sediments at the Guaymas Basin for 8 days and 22 days. Each AISICS module contained 18 colonizers (including sterile controls) filled with basaltic glasses of contrasting composition. Chemical analyses of ambient fluids sampled through the colonizers showed a greater contribution of...
Tipo: Text
Palavras-chave: Colonization module; Basalt alteration; Guaymas basin; Organic-rich sediment; Hydrothermal systems.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00181/29261/27642.pdf

Specific enrichment of hyperthermophilic electroactive Archaea from deep-sea hydrothermal vent on electrically conductive support.
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pillot, Guillaume; Frouin, Eleonore; Pasero, Emilie; Godfroy, Anne; Combet-blanc, Yannick; Davidson, Sylvain; Liebgott, Pierre-pol.
While more and more investigations are done to study hyperthermophilic exoelectrogenic communities from environments, none have been performed yet on deep-sea hydrothermal vent. Samples of black smoker chimney from Rainbow site on the Atlantic mid-oceanic ridge have been harvested for enriching exoelectrogens in microbial electrolysis cells under hyperthermophilic (80 °C) condition. Two enrichments were performed in a BioElectrochemical System specially designed: one from direct inoculation of crushed chimney and the other one from inoculation of a pre-cultivation on iron (III) oxide. In both experiments, a current production was observed from 2.4 A/m2 to 5.8 A/m2 with a set anode potential of -0.110 V vs Ag/AgCl. Taxonomic affiliation of the exoelectrogen...
Tipo: Text
Palavras-chave: Hyperthermophilic exoelectrogens; Deep-sea hydrothermal vent; Microbial electrolysis cell; External electron transfer.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00431/54228/55551.pdf

Entrapment of anaerobic thermophilic and hyperthermophilic marine microorganisms in a gellan/xanthan matrix
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Landreau, M.; Duthoit, Frederique; Claeys-bruno, M.; Vandenabeele-trambouze, O.; Aubry, T.; Godfroy, Anne; Le Blay, Gwenaelle.
Aims  The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine micro-organisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and (iii) to validate the method with batch cultures. Methods and Results  The best conditions for immobilization were obtained at 80°C with gellan and xanthan gums. After 5-week incubation, beads showed a good resistance to all tested conditions except those simultaneously including high temperature (100°C), low NaCl (&lt;0∙5 mol l−1) and extreme pH (4/8). To confirm the method efficiency, batch cultures with immobilized Thermosipho sp. strain AT1272 and Thermococcus kodakarensis strain KOD1 showed an absence of detrimental effect...
Tipo: Text
Palavras-chave: (hyper)thermophilic marine micro-organisms; Anaerobiosis; Entrapment; Gellan; Immobilization; Xanthan.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00332/44352/44000.pdf

Export of deep-sea hydrothermal particles, indigenous thermophilic microorganisms and larvae to the surrounding Ocean
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lesongeur, Francoise; Briand, Patrick; Godfroy, Anne; Crassous, Philippe; Byrne, Nathalie; Khripounoff, Alexis.
To assess the production and the export of particulate and biological material in deep-sea hydrothermal vents, four moorings with sediment traps and current meters were deployed for 24 days in the Azores Triple Junction (ATJ) region. They were deployed along an axis starting at the base of a hydrothermal vent chimney and ending 1000 m away. The particles sampled at the base of the chimney were characterized by high concentrations in total sulfur (16%) and iron (4%). The particle composition changed drastically with distance from the vent: the sulfur concentration decreased with an increase in clay components. Thermophilic microorganisms were successfully enriched from all the particle samples. Cultured strains were closely related to hydrothermal species,...
Tipo: Text
Palavras-chave: Deep-sea hydrothermal vents; Particle flux; Hydrothermal microorganisms; Larvae; Regional index: North Atlantic; Mid-Atlantic Ridge; Azores Triple Junction.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00230/34111/33046.pdf

Isolation and physiological characterization of two novel, piezophilic, thermophilic chemolithoautotrophs from a deep-sea hydrothermal vent chimney
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Takai, Ken; Miyazaki, Masayuki; Hirayama, Hisako; Nakagawa, Satoshi; Querellou, Joel; Godfroy, Anne.
P&gt;Two novel, thermophilic piezophiles, capable of chemolithoautotrophic growth, are successfully cultivated and isolated from a black smoker chimney at the TAG field (Mid Atlantic Ridge: MAR) by using a piezophilic cultivation technique. Both strains (strains 106 and 108) represent dominant cultivated populations of the microbial communities in the chimney surface habitat. Strain 106 represents typically thin, long spiral cells under the piezophilic growth condition but short bent cells under the non-piezophilic condition. It is a strictly chemolithoautotrophic gammaproteobacterium using reduced sulfur compounds as the electron donors, and nitrate and O-2 as the electron acceptors. Based on the 16S rRNA gene sequence, strain 106 would represent a novel...
Tipo: Text
Palavras-chave: Sp. nov.; Ecological signifiance; Hydrostatic pressure; Subsurface biosphere; Base composition; High temperature; Membrane lipids; Gen. nov.; Microorganisms; Diversity.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6652.pdf

Microorganisms persist at record depths in the subseafloor of the Canterbury Basin
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ciobanu, Maria Cristina; Burgaud, Gaetan; Dufresne, Alexis; Breuker, Anja; Redou, Vanessa; Ben Maamar, Sarah; Gaboyer, Frederic; Vandenabeele-trambouze, Odile; Lipp, Julius Sebastian; Schippers, Axel; Vandenkoornhuyse, Philippe; Barbier, Georges; Jebbar, Mohamed; Godfroy, Anne; Alain, Karine.
The subsurface realm is colonized by microbial communities to depths of &gt;1000 meters below the seafloor (m.b.sf.), but little is known about overall diversity and microbial distribution patterns at the most profound depths. Here we show that not only Bacteria and Archaea but also Eukarya occur at record depths in the subseafloor of the Canterbury Basin. Shifts in microbial community composition along a core of nearly 2 km reflect vertical taxa zonation influenced by sediment depth. Representatives of some microbial taxa were also cultivated using methods mimicking in situ conditions. These results suggest that diverse microorganisms persist down to 1922 m.b.sf. in the seafloor of the Canterbury Basin and extend the previously known depth limits of...
Tipo: Text
Palavras-chave: Deep biosphere; Subsurface life; Eukaryote; Record depth.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00201/31243/29895.pdf

Influence of DNA Extraction Method, 16S rRNA Targeted Hypervariable Regions, and Sample Origin on Microbial Diversity Detected by 454 Pyrosequencing in Marine Chemosynthetic Ecosystems
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cruaud, Perrine; Vigneron, Adrien; Lucchetti-miganeh, Celine; Ciron, Pierre Emmanuel; Godfroy, Anne; Cambon-bonavita, Marie-anne.
Next-generation sequencing (NGS) opens up exciting possibilities for improving our knowledge of environmental microbial diversity, allowing rapid and cost-effective identification of both cultivated and uncultivated microorganisms. However, library preparation, sequencing, and analysis of the results can provide inaccurate representations of the studied community compositions. Therefore, all these steps need to be taken into account carefully. Here we evaluated the effects of DNA extraction methods, targeted 16S rRNA hypervariable regions, and sample origins on the diverse microbes detected by 454 pyrosequencing in marine cold seep and hydrothermal vent sediments. To assign the reads with enough taxonomic precision, we built a database with about 2,500...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00204/31495/29907.pdf

Cultivation of an immobilized (hyper)thermophilic marine microbial community in a bioreactor
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Landreau, M.; Duthoit, Frederique; Roussel, Erwan; Schonherr, S.; Georges, Myriam; Godfroy, Anne; Le Blay, G.
Cultivation in a bioreactor of immobilized deep-sea hydrothermal microbial community was tested in order to assess the stability and reactivity of this new system. A community composed of 8 hydrothermal strains was entrapped in a polymer matrix that was used to inoculate a continuous culture in a gas-lift bioreactor. The continuous culture was performed for 41 days at successively 60°C, 55°C, 60°C, 85°C and 60°C, at pH 6.5, in anaerobic condition and constant dilution rate. Oxic stress and pH variations were tested at the beginning of the incubation. Despite these detrimental conditions, 3 strains including 2 strict anaerobes were maintained in the bioreactor. High cell concentrations (3 × 108 cells mL−1) and high ATP contents were measured in both liquid...
Tipo: Text
Palavras-chave: Immobilization; Anaerobiosis; Marine (hyper)thermophiles; Continuous culture.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00347/45851/45516.pdf

Comparative study of vent and seep macrofaunal communities in the Guaymas Basin
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Portail, Marie; Olu, Karine; Escobar-briones, E.; Caprais, Jean-claude; Menot, Lenaick; Waeles, Mathieu; Cruaud, Perrine; Sarradin, Pierre-marie; Godfroy, Anne; Sarrazin, Jozee.
Understanding the ecological processes and connectivity of chemosynthetic deep-sea ecosystems requires comparative studies. In the Guaymas Basin (Gulf of California, Mexico), the presence of seeps and vents in the absence of a biogeographic barrier, and comparable sedimentary settings and depths offers a unique opportunity to assess the role of ecosystem-specific environmental conditions on macrofaunal communities. Six seep and four vent assemblages were studied, three of which were characterised by common major foundation taxa: vesicomyid bivalves, siboglinid tubeworms and microbial mats. Macrofaunal community structure at the family level showed that density, diversity and composition patterns were primarily shaped by seep- and vent-common abiotic...
Tipo: Text
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00280/39076/37642.pdf

Marinitoga camini gen, nov., sp nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Wery, Nathalie; Lesongeur, Francoise; Pignet, Patricia; Derennes, Veronique; Cambon-bonavita, Marie-anne; Godfroy, Anne; Barbier, Georges.
A thermophilic, anaerobic, chemo-organotrophic sulfur-reducing bacterium, designated MV1075(T), was isolated from a deep-sea hydrothermal chimney sample collected on the Mid-Atlantic Ridge. Cells were rod-shaped with a sheath-like outer structure, motile with polar flagella and stained Gramnegative. They appeared singly, in pairs or in short chains. The temperature range for growth was 25-65 degreesC, with an optimum at 55 degreesC. Growth was observed from ph 5 to ph 9, and the optimum ph was around 7. The salinity range for growth was 15-70 g sea salt I-1 (corresponding to 10-45 g NaCl I-1), with an optimum at 30 g I-1 (20 g NaCl I-1). The isolate was able to grow on a broad spectrum of carbohydrates or complex proteinaceous substrates. Sulfur was not...
Tipo: Text
Palavras-chave: Marinitoga camini; Thermotogales; Thermophile; Deep sea hydrothermal vent.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-587.pdf

Cartographie haute résolution des champs hydrothermaux de la ride sud du bassin de Guaymas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ondreas, Helene; Scalabrin, Carla; Fouquet, Yves; Godfroy, Anne.
Insights into the factors controlling fluid circulation through the crust and the nature of fluid venting at the seafloor are first steps in understanding their effect on ocean properties and climate change. New data on the seafloor morphology, sub-surface sedimentary stratification, and water column of the sedimented Southern Trough hydrothermal field (Guaymas basin) were acquired during the BIG cruise in 2010. These data provide accurate and high-resolution information on the geological context of the vents, on the distribution of acoustic anomalies in the water column, and on the possible nature of the fluid generating these echoes. More than 40 hydrothermal edifices were observed. The southern zone of the study area hosts hydrothermal sites that differ...
Tipo: Text
Palavras-chave: Mid-ocean ridges; Sedimented ridge; Hydrothermal systems; Fluid migration; Water-column acoustic images; Guaymas basin.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00436/54775/56229.pdf

Optimisation of growth conditions for continuous culture of the hyperthermophilic archaeon Thermococcus hydrothermalis and development of sulphur-free defined and minimal media
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Postec, Anne; Pignet, Patricia; Cueff-gauchard, Valerie; Schmitt, Anne; Querellou, Joel; Godfroy, Anne.
The hyperthermophilic archaeon Thermococcus hydrothermalis was cultivated in continuous culture in a gas-lift bioreactor in the absence of elemental sulphur on both proteinaceous and maltose-containing media. Optimal conditions (pH, temperature and gas flow rate), determined on complex media that yielded maximal growth rate and maximal steady state cell density, were obtained at 80degreesC, pH 6 and gas sparging at 0.2 v v(-1) min(-1). Higher steady state cell densities were obtained on a medium containing maltose and yeast extract. In order to design a defined and minimal media, the nutritional requirements of T hydrothermalis were then investigated using continuous culture in the absence of elemental sulphur in the gas-lift bioreactor. First, the complex...
Tipo: Text
Palavras-chave: Bioreactor; Defined and minimal media; Culture optimisation; Gas lift bioreactor; Thermococcus hydrothermalis.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-365.pdf

Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Vigneron, Adrien; L Haridon, Stephane; Godfroy, Anne; Roussel, Erwan; Cragg, Barry A.; Parkes, R. John; Toffin, Laurent.
In the Sonora Margin cold seep ecosystems (Gulf of California), sediments underlying microbial mats harbor high biogenic methane concentrations, fueling various microbial communities, such as abundant lineages of anaerobic methanotrophs (ANME). However, the biodiversity, distribution, and metabolism of the microorganisms producing this methane remain poorly understood. In this study, measurements of methanogenesis using radiolabeled dimethylamine, bicarbonate, and acetate showed that biogenic methane production in these sediments was mainly dominated by methylotrophic methanogenesis, while the proportion of autotrophic methanogenesis increased with depth. Congruently, methane production and methanogenic Archaea were detected in culture enrichments amended...
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00256/36737/35338.pdf

Comparative Study of Guaymas Basin Microbiomes: Cold Seeps vs. Hydrothermal Vents Sediments
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cruaud, Perrine; Vigneron, Adrien; Pignet, Patricia; Caprais, Jean-claude; Lesongeur, Francoise; Toffin, Laurent; Godfroy, Anne; Cambon-bonavita, Marie-anne.
In the Guaymas Basin, the presence at a few tens of kilometers of cold seeps and hydrothermal vents coupled with comparable sedimentary settings and depths offer a unique opportunity to assess and compare the microbial community composition of these deep-sea ecosystems. The microbial diversity in sediments from three cold seep and two hydrothermal vent assemblages were investigated using high-throughput 16S rRNA-sequencing. Numerous bacterial and archaeal lineages were detected in both cold seep and hydrothermal vent sediments. Various potential organic matter degraders (e.g., Chloroflexi, Atribacteria, MBG-D) and methane and sulfur cycling related microorganisms (e.g., ANME and methanogenic lineages, sulfate-reducing lineages) were detected in both...
Tipo: Text
Palavras-chave: Microbial diversity; Hydrothermal vents; Cold seeps; Ribotypes; Methane; Extreme environments.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00415/52640/53496.pdf

Growth of hyperthermophilic microorganisms for physiological and nutritional studies
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Godfroy, Anne; Postec, Anne; Raven, N.
Tipo: Text
Palavras-chave: BACTERIAL GROWTH; SCALE CULTIVATION; ORDER THERMOTOGALES; TEMPERATURE BIOREACTOR; HYDROSTATIC PRESSURE; CONTINUOUS CULTURE; PYROCOCCUS FURIOSUS; SP NOV; ARCHAEON THERMOCOCCUS LITORALIS; SEA HYDROTHERMAL VENT.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-3890.pdf

Microbial communities associated with benthic faunal assemblages at cold seep sediments of the Sonora Margin, Guaymas Basin
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cruaud, Perrine; Vigneron, Adrien; Pignet, Patricia; Caprais, Jean-claude; Lesongeur, Francoise; Toffin, Laurent; Godfroy, Anne; Cambon-bonavita, Marie-anne.
The Sonora Margin cold seeps present a seafloor mosaic pattern consisting of different faunal assemblages and microbial mats. To better understand if sedimentary microbial communities reflect this patchy distribution, all major habitats were investigated using four complementary approaches: 16S rRNA gene sequence 454 pyrosequencing, quantitative polymerase chain reaction, fluorescence in situ hybridization and geochemistry analyses. This study reveals that sediments populated by different surface assemblages show distinct porewater geochemistry features and are associated with distinct microbial communities. In the sediments underlying the microbial mat and the surrounding macrofauna, microbial communities were dominated by anaerobic methane oxidizers...
Tipo: Text
Palavras-chave: Microbial communities; Pyrosequencing; MBG-D; ANME; Faunal assemblages; Vesicomyidae; Siboglinidae; Microbial mat.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00288/39889/38434.pdf

Draft Genome Sequences of Two  Marinitoga camini  Isolates Producing Bacterioviruses
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Mercier, Coraline; Lossouarn, Julien; Haverkamp, Thomas; Bienvenu, Nadège; Godfroy, Anne; Cueff-gauchard, Valerie; Geslin, Claire; Nesbo, Camilla.
Here, we present the draft genome sequences of two thermophilic Marinitoga strain members of the Thermotogales order, Marinitoga camini DV1155 and Marinitoga camini DV1197. These strains were isolated from deep-sea hydrothermal vents of the Mid-Atlantic Ridge.
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00357/46833/46708.pdf

Effect of variation of environmental conditions on the microbial communities of deep-sea vent chimneys, cultured in a bioreactor
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Byrne, Nathalie; Lesongeur, Francoise; Bienvenu, Nadege; Geslin, Claire; Alain, Karine; Prieur, Daniel; Godfroy, Anne.
Both cultivation and molecular techniques were used to investigate the microbial diversity and dynamic of a deep-sea vent chimney. The enrichment cultures performed in a gas-lift bioreactor were inoculated with a black smoker chimney sample collected on TAG site on the mid-Atlantic ridge. To mimic as close as possible environmental conditions, the cultures were performed in oligotrophic medium with nitrogen, hydrogen and carbon dioxide (N-2/H-2/CO2) gas sweeping. Also, the temperature was first settled at a temperature of 85A degrees C and colloidal sulphur was added. Then, the temperature was lowered to 60A degrees C and sulphur was omitted. Archaeal and bacterial diversity was studied in both culture and natural samples. Through 16S rRNA gene sequences...
Tipo: Text
Palavras-chave: 16S rRNA genes; Oligotrophic condition; Environmental condition; Bioreactor; Continuous enrichment culture; Microbial diversity; Deep sea hydrothermal vent.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6920.pdf

An Extended Network of Genomic Maintenance in the Archaeon Pyrococcus abyssi Highlights Unexpected Associations between Eucaryotic Homologs
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pluchon, Pierre Francois; Fouqueau, Thomas; Creze, Christophe; Laurent, Sebastien; Briffotaux, Julien; Hogrel, Gaelle; Palud, Adeline; Henneke, Ghislaine; Godfroy, Anne; Hausner, Winfried; Thomm, Michael; Nicolas, Jacques; Flament, Didier.
In Archaea, the proteins involved in the genetic information processing pathways, including DNA replication, transcription, and translation, share strong similarities with those of eukaryotes. Characterizations of components of the eukaryotic-type replication machinery complex provided many interesting insights into DNA replication in both domains. In contrast, DNA repair processes of hyperthermophilic archaea are less well understood and very little is known about the intertwining between DNA synthesis, repair and recombination pathways. The development of genetic system in hyperthermophilic archaea is still at a modest stage hampering the use of complementary approaches of reverse genetics and biochemistry to elucidate the function of new candidate DNA...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00171/28198/26556.pdf

Complete Genome Sequence of the Hyperthermophilic Piezophilic Archaeon   Pyrococcus kukulkanii   NCB100 Isolated from the Rebecca’s Roost Hydrothermal Vent in the Guaymas Basin
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Oger, Philippe M.; Callac, Nolwenn; Oger-desfeux, Christine; Hughes, Sandrine; Gillet, Benjamin; Jebbar, Mohamed; Godfroy, Anne.
Members of the order Thermococcales are common inhabitants of high-temperature hydrothermal vent systems (black smokers) that are represented in clone libraries mostly by isolates from the Thermococcus genus. We report the complete sequence of a novel species from the Pyrococcus genus, P. kukulkanii strain NCB100, which has been isolated from a flange fragment of the Rebecca’s Roost hydrothermal vent system in the Guaymas Basin.
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00476/58798/61335.pdf

Pyrococcus horikoshii TET2 Peptidase Assembling Process and Associated Functional Regulation
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Appolaire, Alexandre; Rosenbaum, Eva; Dura, M. Asuncion; Colombo, Matteo; Marty, Vincent; Savoye, Marjolaine Noirclerc; Godfroy, Anne; Schoehn, Guy; Girard, Eric; Gabel, Frank; Franzetti, Bruno.
Tetrahedral (TET) aminopeptidases are large polypeptide destruction machines present in prokaryotes and eukaryotes. Here, the rules governing their assembly into hollow 12-subunit tetrahedrons are addressed by using TET2 from Pyrococcus horikoshii (PhTET2) as a model. Point mutations allowed the capture of a stable, catalytically active precursor. Small angle x- ray scattering revealed that it is a dimer whose architecture in solution is identical to that determined by x- ray crystallography within the fully assembled TET particle. Small angle x- ray scattering also showed that the reconstituted PhTET2 dodecameric particle displayed the same quaternary structure and thermal stability as the wild-type complex. The PhTET2 assembly intermediates were...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00177/28820/27491.pdf

Bacterial communities and syntrophic associations involved in anaerobic oxidation of methane process of the Sonora Margin cold seeps, Guaymas Basin
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Vigneron, Adrien; Cruaud, Perrine; Pignet, Patricia; Caprais, Jean-claude; Gayet, Nicolas; Cambon-bonavita, Marie-anne; Godfroy, Anne; Toffin, Laurent.
The Sonora Margin cold seeps present on the seafloor a patchiness pattern of white microbial mats surrounded by polychaete and gastropod beds. These surface assemblages are fuelled by abundant organic inputs sedimenting from the water column and upward-flowing seep fluids. Elevated microbial density was observed in the underlying sediments. A previous study on the same samples identified anaerobic oxidation of methane (AOM) as the potential dominant archaeal process in these Sonora Margin sediments, probably catalysed by three clades of archaeal anaerobic methanotrophs (ANME-1, ANME-2 and ANME-3) associated with bacterial syntrophs. In this study, molecular surveys and microscopic observations investigating the diversity of Bacteria involved in AOM...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00168/27921/27279.pdf

Continuous enrichment cultures: insights into prokaryotic diversity and metabolic interactions in deep-sea vent chimneys
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Postec, Anne; Lesongeur, Francoise; Pignet, Patricia; Ollivier, B; Querellou, Joel; Godfroy, Anne.
The prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. Cultures were conducted at 60 degrees C and pH 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the Rainbow field, Mid-Atlantic Ridge, at 2,275 m depth. To assess the relevance of both culture methods, bacterial and archaeal diversity was studied using cloning and sequencing, DGGE, and whole-cell hybridisation of 16S rRNA genes. Sequences of heterotrophic microorganisms belonging to the genera Marinitoga,...
Tipo: Text
Palavras-chave: Metabolic interactions; 16S rRNA gene; Thermophiles; Bioreactor; Continuous enrichment cultures; Deep sea hydrothermal vent; Microbial diversity.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3885.pdf

Continuous enrichment culture and molecular monitoring to investigate the microbial diversity of thermophiles inhabiting deep-sea hydrothermal ecosystems
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Postec, Anne; Urios, Laurent; Lesongeur, Francoise; Ollivier, Bernard; Querellou, Joel; Godfroy, Anne.
The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90 degrees C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA. Additional sequences phylogenetically related to Thermococus and epsilon-Proteobacteria were detected by cloning and sequencing of 16S rDNA from two samples of the enrichment culture. In comparison, the sequences retrieved from cloning analysis from an enrichment culture...
Tipo: Text
Palavras-chave: Sequence alignment; Anaerobic digester; Thermotogales; Thermophile; Deep sea vent.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-364.pdf

Vallitalea guaymasensis gen. nov., sp nov., isolated from marine sediment
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lakhal, Raja; Pradel, Nathalie; Postec, Anne; Hamdi, Moktar; Ollivier, Bernard; Godfroy, Anne; Fardeaul, Marie-laure.
A novel obligately anaerobic, non-spore-forming, rod-shaped mesophilic, halophilic, Gram-stain-negative bacterium, was isolated from sediments of Guaymas Basin. The strain, designated Ra1766G1(T), grew at 20-40 degrees C (optimum, 30-35 degrees C) and at pH 6.0-8.0 (optimum, pH 6.5-7.5). It required 0.5-7.5 % NaCl (optimum, 2-3 %) for growth. Sulfate, thiosulfate, elemental sulfur, sulfite, fumarate, nitrate and nitrite were not used as terminal electron acceptors. Strain Ra1766G1(T) used cellobiose, glucose, mannose, maltose, arabinose, raffinose, galactose, ribose, sucrose, pyruvate and xylose as electron donors. The main fermentation product from glucose metabolism was acetate. The predominant cellular fatty acids were anteiso-C-15:0, iso-C-15:0,...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00177/28821/27463.pdf

Physiology and continuous culture of the hyperthermophilic deep-sea vent archaeon Pyrococcus abyssi ST549
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Godfroy, Anne; Raven, N; Sharp, R.
The deep-sea vent archaeon Pyrococcus abyssi strain ST549 was grown in batch cultures in closed bottles and by continuous culture in a gas-lift bioreactor, both in the presence and in the absence of elemental sulfur. Growth on carbohydrates, proteinaceous substrates and amino acids was investigated. The disaccharides maltose and cellobiose were shown not to be able to enhance growth suggesting that P. abyssi ST549 is unable to use them as carbon sources. By contrast, proteinaceous substrates such as peptone and brain heart infusion were shown to be very good substrates for the growth of P. abyssi ST549 and allowed growth at high steady-state cell densities in continuous culture. Growth on brain heart infusion was shown to require additional nutrients when...
Tipo: Text
Palavras-chave: Sulfur requirement; Continuous culture; Nutrition; Hyperthermophile; Pyrococcus abyssi.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-1087.pdf