Consulta: creatorFacets:"Ky, Chin-long"
Registros recuperados: 40
Data/hora: 11/06/2024 14:51:14

Characterization of molecular processes involved in the pearl formation in Pinctada margaritifera for a sustainable development of pearl farming industry in French Polynesia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Gueguen, Yannick; Montagnani, Caroline; Joubert, Caroline; Marie, Benjamin; Belliard, Corinne; Tayale, Alexandre; Fievet, Julie; Levy, Peva; Piquemal, David; Marin, Frederic; Le Moullac, Gilles; Ky, Chin-long; Garen, Pierre; Lo, Cedrick; Saulnier, Denis.
Tahiti’s pearl farming industry plays a major socio-economic role in French Polynesia. In an increasingly competitive market where the production of high quality pearls becomes essential, research can help secure and ensure sustainable production. In that context, Ifremer, in close collaboration with the “direction des resources marines” (French Polynesian government agency) has developed research projects on the “sustainable development of pearl farming”. This program is organized along 3 axes: (1) understanding the animal physiology and initiating a genetically selective breeding program of donor oysters; (2) understanding pearl oyster larvae dispersal and recruitment; (3) understanding the molecular mechanisms underlying biomineralization processes...
Tipo: Text
Palavras-chave: Pearl oyster; Biomineralization; Pinctada margaritifera.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00167/27840/26049.pdf

Relationship of the orange tissue morphotype with shell and pearl colouration in the mollusc Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Blay, Carole; Broustal, Floriane; Sham Koua, Manaarii; Planes, Serge.
Molluscs display a vast range of shell colours both between and within species. However, only a few species show colour variation in their soft tissues. In French Polynesia, the pearl oyster Pinctada margaritifera has three tissue morphotypes: the black wild-type and two rare mutations: white albino and orange mantle. Phenotypic transmission is known to occur from these phenotypes when they are used as graft donors for pearl production, leading to multicoloured and white pearls from black and albino mantle grafts, respectively. The present study furthers this knowledge by examining the phenotypic association between the orange mantle tissue morphotype and hard tissues: shells and cultured pearls. Based on a large experimental graft, shell colour...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00486/59760/62878.pdf

Potential combinations of mabé, keshi and cultured pearl production from colourful hatchery-produced Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Leclerc, Nicolas; Broustal, Floriane; Nakasai, Seiji; Devaux, Dominique.
Aquaculture of nacreous gems, such as cultured pearls, mabé or keshi, is done mostly using different mollusc species grown in countries of the Indo-Pacific region. To date, no single species has been exploited for the simultaneous generation of more than one of these bioproducts, but all require animals with colourful shells. Historically, Pinctada species have mainly been used for nucleated pearl production, selecting the rarer colourful individuals to be used as graft donors. By contrast, colourful Pteria species have mostly been used for mabé production, as the grafting operation for pearl production is associated with low yield. In this study, we report the potential for cumulating cultured pearl and mabé (MP), or keshi and mabé (MK) production, using...
Tipo: Text
Palavras-chave: Pearl oyster; Pinctada margaritifera; Mabe; Keshi; Pearls.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00482/59398/62168.pdf

Evidence of donor effect on cultured pearl quality from a duplicated grafting experiment on Pinctada margaritifera using wild donors
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Tayale, Alexandre; Gueguen, Yannick; Treguier, Cathy; Le Grand, Jacqueline; Cochennec-laureau, Nathalie; Montagnani, Caroline; Ky, Chin-long.
Producing high quality cultured black pearls from Pinctada margaritifera is one of the major challenges for the "pearl oyster" industry in French Polynesia. In order to assess donor effect on cultured pearl quality, wild Pinctada margaritifera originating from the Tuamotu Archipelago were used in a duplicated grafting experiment. After 12 months of culture, nucleus retention was assessed and seven pearl quality traits recorded on the 454 cultured pearls harvested from the experiment. The traits scored were nacre thickness and pearl weight, surface defects, lustre, grade, and the colour components: 1) darkness of cultured pearl colour, and 2) visual perception of colour class (bodycolor and/or overtone). Our results demonstrate for the first time that...
Tipo: Text
Palavras-chave: Cultured pearl oyster; Pearl quality; Nacre; Donor mollusc; Genetic selection; Pteriidae; Blacklip pearl oyster; Pinctada margaritifera; French Polynesia.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00116/22757/20541.pdf

Tracing key genes associated with the Pinctada margaritifera albino phenotype from juvenile to cultured pearl harvest stages using multiple whole transcriptome sequencing
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Auffret, Pauline; Le Luyer, Jeremy; Sham Koua, Manaarii; Quillien, Virgile; Ky, Chin-long.
Background Albino mutations are commonly observed in the animal kingdom, including in bivalves. In the black-lipped pearl oyster Pinctada margaritifera, albino specimens are characterized by total or partial absence of colouration resulting in typical white shell phenotype expression. The relationship of shell colour with resulting cultured pearl colour is of great economic interest in P. margaritifera, on which a pearl industry is based. Hence, the albino phenotype provides a useful way to examine the molecular mechanisms underlying pigmentation. Results Whole transcriptome RNA-sequencing analysis comparing albino and black wild-type phenotypes at three stages over the culture cycle of P. margaritifera revealed a total of 1606, 798 and 187 differentially...
Tipo: Text
Palavras-chave: Pinctada margaritifera; Albinism; Pigmentation; RNA-seq; Notch signaling pathway; Tyrosinase; Biomineralization.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00652/76420/77477.pdf

Low energy cost for cultured pearl formation in grafted chimeric Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Le Moullac, Gilles; Soyez, Claude; Ky, Chin-long.
The pearl oyster is one of the rare animal models that support two distinct genomes,  through the surgical graft process operated for culture pearl production. This grafted  organism is assimilated to a chimera whose physiological functioning remains poorly known.  The question of the energy expenditure comparison between chimera and non-chimera  animals arises. To answer this question, grafted and non-grafted pearl oysters were  evaluated for their energetic needs by the indirect calorimetry method. This method made it  possible to measure the energy expenditure based on the respiration rate (RR)  measurement, reflecting the basal metabolism. The results showed that the RR values for  grafted and non-grafted pearl oysters were not significantly different...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00437/54861/56350.pdf

Development of cultured pearl circles and shape after initial graft and second nucleus insertion in the black-lipped pearl oyster Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Demmer, Jonathan; Sham-koua, Manaarii; Cabral, Philippe.
Production of larger, rounder, high-quality cultured pearls with fewer circles is one of the main challenges of Pinctada margaritifera aquaculture faced by every pearl farm in French Polynesia. Although bigger pearl sizes can be achieved through surgreffe operations (implantation of a second nucleus following pearl harvest), control of the development of pearl circles and shapes still remains unclear, as illustrated by grafter’s empirical rules, where often the surgreffe process is only performed after production of uncircled and round pearl shapes. The present study was designed with a real pearl by pearl traceability to reveal for the first time the development of circles and shapes from graft and surgreffe, in relation to the size of the pearl sac. This...
Tipo: Text
Palavras-chave: Pearl oyster; Pinctada margaritifera; Surgreffe; Circled pearls; Pearl shapes; Pearl sac.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00276/38690/37187.pdf

Impact of spat shell colour selection in hatchery-produced Pinctada margaritifera on cultured pearl colour
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Sham Koua, Manaarii; Le Moullac, Gilles.
Beaded cultured pearl farming is a lengthy aquaculture process, particularly when the pearl oysters are produced through a hatchery propagation system, and includes the key steps of artificial breeding, larval and spat rearing before graft operations can take place. Within its genus, Pinctada margaritifera has the ability to produce the widest range of pearl colours, thanks to the donor colour polymorphism of the inner shell, which is mainly responsible for colour transmission. As hatchery spat production in P. margaritifera leads to several colour phenotypes (at 3 months old), the aim of this study was to determine whether a relation exists between the colour of the donors as spat and the final pearl colour. In the experiment, which took place over a...
Tipo: Text
Palavras-chave: Pinctada margaritifera; Phenotypes; Spat shell colour; Earlier selection; Pearl colour.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00418/52969/53895.pdf

Colour plasticity in the shells and pearls of animal graft model Pinctada margaritifera assessed by HSV colour quantification
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Stenger, Pierre-louis; Vidal-dupiol, Jeremie; Reisser, Celine; Planes, Serge; Ky, Chin-long.
The bivalve Pinctada margaritifera has the capacity to produce the most varied and colourful pearls in the world. Colour expression in the inner shell is under combined genetic and environmental control and is correlated with the colour of pearls produced when the same individual is used as a graft donor. One major limitation when studying colour phenotypes is grader subjectivity, which leads to inconsistent colour qualification and quantification. Through the use of HSV (Hue Saturation Value) colour space, we created an R package named ‘ImaginR’ to characterise inner shell colour variations in P. margaritifera. Using a machine-learning protocol with a training dataset, ImaginR was able to reassign individual oysters and pearls to predefined human-based...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00497/60900/64239.pdf

Growth Performance Comparison of Pinctada margaritifera Juveniles Produced by Thermal Shock or Gonad Scarification Spawning Procedures
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Lau, Christophe; Sham Koua, Manaarii; Lo, Cedrik.
The black-lipped pearl oyster Pinctada margaritifera is of high economic importance in French Polynesia. Arecent decline in pearl value led to the initiation of several genetic breeding programs aiming to improve production traits, including oyster shell growth, which dictates the time of grafting, size of the implanted nuclei, and biomineralization capacities. We assessed shell diameters on hatchery-produced spat to analyze juvenile growth performance of four half-sib families derived from polyandry (one dam crossed with two sires) and polygyny (two dams crossed with one sire) using gametes obtained by thermal shock or gonad scarification. Spat growth was monitored over 3 mo, with shell diameter measured weekly. Results revealed that the spawning method...
Tipo: Text
Palavras-chave: Pearl oysters; Pinctada margaritifera; Juvenile; Growth performance; Spawning procedure.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00310/42167/41470.pdf

Strong genetic isolation of the black-lipped pearl oyster (Pinctada margaritifera) in the Marquesas archipelago (French Polynesia)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Reisser, Celine; Lo, Cédrik; Schikorski, David; Sham Koua, Manaarii; Planes, Serge; Ky, Chin-long.
The French Polynesian islands are internationally known for their black pearls, produced by culture of the black lipped pearl oyster Pinctada margaritifera. The ongoing development of hatcheries for P. margaritifera in French Polynesia poses new challenges for the industry, particularly regarding the maintenance of genetic diversity in the hatchery stocks. This emphasizes the necessity to characterize the genetic diversity and differentiation within natural and exploited populations, to carefully select putative parental populations. The present study aimed at validating the phylogenetic status and investigating genetic attributes of populations from the only two non-exploited archipelagos of French Polynesia, the Marquesas archipelago, and the Australes...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00509/62059/66234.pdf

Temperature and Food Influence Shell Growth and Mantle Gene Expression of Shell Matrix Proteins in the Pearl Oyster Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Joubert, Caroline; Linard, Clementine; Le Moullac, Gilles; Soyez, Claude; Saulnier, Denis; Teaniniuraitemoana, Vaihiti; Ky, Chin-long; Gueguen, Yannick.
In this study, we analyzed the combined effect of microalgal concentration and temperature on the shell growth of the bivalve Pinctada margaritifera and the molecular mechanisms underlying this biomineralization process. Shell growth was measured after two months of rearing in experimental conditions, using calcein staining of the calcified structures. Molecular mechanisms were studied though the expression of 11 genes encoding proteins implicated in the biomineralization process, which was assessed in the mantle. We showed that shell growth is influenced by both microalgal concentration and temperature, and that these environmental factors also regulate the expression of most of the genes studied. Gene expression measurement of shell matrix protein...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00210/32095/30535.pdf

Phenome of pearl quality traits in the mollusc transplant model Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Quillien, Virgile; Broustal, Floriane; Soyez, Claude; Devaux, Dominique.
The bivalve Pinctada margaritifera exhibits three main transplant phenotypes derived from  the donor (from which a mantle graft tissue, the saibo, is excised), the recipient (into which  the saibo is implanted with a nucleus, leading to the formation of a pearl sac "chimera") and  the cultured pearls themselves. This first phenome study on the species derived from a large  experimental graft. Transplant phenotype was assessed at three scales: 1) macro, pearl size,  colour, grade, 2) micro, pearl surface microstructure, and 3) molecular, biomineralisation  gene expression level in saibo and pearl sac tissues. From donor to pearl, the phenome  revealed fine variations of quality traits dependent on the position on the mantle where the  saibo was cut, whose...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00423/53419/54306.pdf

The pearl oyster (Pinctada margaritifera) aquaculture in French Polynesia and the indirect impact of long-distance transfers and collection-culture site combinations on pearl quality traits
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Broustal, Floriane; Potin, Dan; Lo, Cedrik.
In French Polynesia, the P. margaritifera pearl aquaculture industry is spread over a vast area, as large as Europe. All the oysters for this the highly economically important activity are supplied from just a few collection lagoons, but they are grown in numerous sites across three archipelagos (Gambier, Society and Tuamotu). Many oyster transfers thus indirectly bring about grafting combinations mixing different geographic origins and production sites. This study aims to examine the impact of such graft combinations on cultured pearl quality traits. For this, six homogeneous and standardised experimental graft combinations (N = 6197) were conducted at commercial scale in the two growing locations the most frequently used in French Polynesia: Arutua atoll...
Tipo: Text
Palavras-chave: Pearl oyster; Pinctada margaritifera; Graft combinations; Pearl quality traits; Environment.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00477/58888/61444.pdf

Genetic investigations on the caffeine and chlorogenic acid relationship in an interspecific cross between Coffea liberica dewevrei and C-pseudozanguebariae
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Barre, Philippe; Noirot, Michel.
The objective of the paper was to identify the number of major loci explaining caffeine content in coffee seeds. Investigations were based on previously published results: (1) Caffeine binds to chlorogenic acids in a 1:1 molecular ratio; (2) Between species, the caffeine content is correlated to the chlorogenic acid content; (3) Only a part of chlorogenic acids is bound to caffeine. Especially, the content ratio between caffeine and chlorogenic acids varied between species. For identifying the number of major loci, a quantitative trait locus (QTL) approach was carried out using an interspecific cross between two highly differentiated species-Coffea liberica dewevrei and Coffea pseudozanguebariae, the latter being a caffeine-free species. As main finding,...
Tipo: Text
Palavras-chave: Coffea; Chlorogenic acids; Caffeine; QTL; Interspecific cross.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00152/26298/24429.pdf

Influence of Temperature and Pearl Rotation on Biomineralization in the Pearl Oyster Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Le Moullac, Gilles; Schuck, Lucie; Chabrier, Sebastien; Belliard, Corinne; Lyonnard, Pierre; Broustal, Floriane; Soyez, Claude; Saulnier, Denis; Brahmi, Chloe; Ky, Chin-long; Beliaeff, Benoit.
The objective of this study was to observe the impact of temperature on pearl formation using  an integrative approach describing the rotation of the pearls, the rate of nacre deposition, the  thickness of the aragonite tablets and the biomineralizing potential of the pearl sac tissue  though the expression level of some key genes. Fifty pearl oysters were grafted with  magnetized nuclei to allow the rotation of the pearls to be described. Four months later, 32 of  these pearl oysters were exposed to four temperatures (22, 26, 30 and 34°C) for 2 weeks.  Results showed that the rotation speed differed according to the movement direction: pearls  with axial movement (AM) had a significantly higher rotation speed than those with random  movement (RM). Pearl...
Tipo: Text
Palavras-chave: Magnetometer; Rotation speed; Nacre growth; Nacre thickness; Gene expression; Pmarg-Pif177.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00450/56196/57753.pdf

Crossing phenotype heritability and candidate gene expression in grafted black-lipped pearl oyster Pinctada margaritifera, an animal chimera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Blay, Carole; Planes, Serge; Ky, Chin-long.
Grafting mantle tissue of a donor pearl oyster into the gonad of a recipient oyster results in the formation of a chimera, the pearl sac. The phenotypic variations of this chimera are hypothesized to be the result of interactions between the donor and recipient genomes. In this study, the heritability of phenotypic variation and its association with gene expression were investigated for the first time during P. margaritifera pearl production. Genetic variance was evaluated at different levels, 1) before the graft operation (expression in graft tissue), 2) after grafting (pearl sac tissue expression in chimera) and 3) on the product of the graft (pearl phenotype traits) based on controlled bi-parental crosses and the F1 generation. Donor related genetic...
Tipo: Text
Palavras-chave: Gene expression; Heritability; Pearl oyster; Phenotype; Pinctada margaritifera.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00433/54505/55876.pdf

Cultured Pearl Surface Quality Profiling by the Shell Matrix Protein Gene Expression in the Biomineralised Pearl Sac Tissue of Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Blay, Carole; Planes, Serge; Ky, Chin-long.
Nucleated pearls are produced by molluscs of the Pinctada genus through the biomineralisation activity of the pearl sac tissue within the recipient oyster. The pearl sac originates from graft tissue taken from the donor oyster mantle and its functioning is crucial in determining key factors that impact pearl quality surface characteristics. The specific role of related gene regulation during gem biogenesis was unknown, so we analysed the expression profiles of eight genes encoding nacreous (PIF, MSI60, PERL1) or prismatic (SHEM5, PRISM, ASP, SHEM9) shell matrix proteins or both (CALC1) in the pearl sac (N = 211) of Pinctada margaritifera during pearl biogenesis. The pearls and pearl sacs analysed were from a uniform experimental graft with sequential...
Tipo: Text
Palavras-chave: Biomineralisation; Relative gene expression; Pearl sac; Biomarkers; Pearl quality; Pinctada margaritifera.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00435/54622/56022.pdf

Environmentally Driven Color Variation in the Pearl Oyster Pinctada margaritifera var. cumingii (Linnaeus, 1758) Is Associated With Differential Methylation of CpGs in Pigment- and Biomineralization-Related Genes
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Stenger, Pierre-louis; Ky, Chin-long; Reisser, Celine; Cosseau, Céline; Grunau, Christoph; Mege, Mickael; Planes, Serge; Vidal-dupiol, Jeremie.
Today, it is common knowledge that environmental factors can change the color of many animals. Studies have shown that the molecular mechanisms underlying such modifications could involve epigenetic factors. Since 2013, the pearl oyster Pinctada margaritifera var. cumingii has become a biological model for questions on color expression and variation in Mollusca. A previous study reported color plasticity in response to water depth variation, specifically a general darkening of the nacre color at greater depth. However, the molecular mechanisms behind this plasticity are still unknown. In this paper, we investigate the possible implication of epigenetic factors controlling shell color variation through a depth variation experiment associated with a DNA...
Tipo: Text
Palavras-chave: Pearl oyster; Environmental pressure; Depth; Color change; Pigmentation; DNA methylation; Methylome characterization.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00685/79667/82436.pdf

Influence of grafter skill and season on cultured pearl shape, circles and rejects in Pinctada margaritifera aquaculture in Mangareva lagoon
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Nakasai, Seiji; Molinari, Nicolas; Devaux, Dominique.
Cultured pearls quality produced by the mollusc Pinctada margaritifera var. cumingi is determined by a panel of criteria, of which shape is one of the most relevant. Cultured pearls with round and semi-round (RS) shapes are the most valuable. Decreasing the proportion of other pearl shapes (OT: baroque, button, drop, oval), and especially the circled pearls (CL) and rejects (rebuts: RT), is an important challenge for the pearl industry. The present study examined the effect of grafter skill and season of graft on the variation in proportions of CL, RS, OT and RT cultured pearl shape categories in a mono-site culture (Mangareva island lagoon). Six large-scale grafting experiments, carried out in the same way as commercial grafting sessions, were made by...
Tipo: Text
Palavras-chave: Pinctada margaritifera; Cultured pearl; Grafter skill; Pearl shapes; Circled pearls; Grafting season.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00216/32745/31150.pdf

Is pearl colour produced from Pinctada margaritifera predictable through shell phenotypes and rearing environments selections?
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Le Pabic, Lore; Koua, Manaarii Sham; Molinari, Nicolas; Nakasai, Seiji; Devaux, Dominique.
The black-lipped pearl oyster, Pinctada margaritifera is the most important farmed mollusc species in French Polynesia. Donor oyster selection among wild P. margaritifera individuals, chosen according to their inner shell colour, makes it possible to obtain the broadest range of cultured pearl colours of any species. This study demonstrates the relative influence of using black [B] or red [R] outer shell phenotypes, combined with green [G] or yellow [Y] inner shell phenotypes, on pearl darkness level, colour categories and lustre. A large scale grafting experiment was designed and carried out over five grow-out locations, covering three archipelagos: Tuamotus, Society and Gambier. Results revealed that the [B + G] phenotypes may be used as donors to...
Tipo: Text
Palavras-chave: Pearl oyster; Pinctada margaritifera; Shell colour phenotype; Pearl colour; Environment.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00285/39637/38129.pdf

Family effect on cultured pearl quality in black-lipped pearl oyster Pinctada margaritifera and insights for genetic improvement
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Blay, Carole; Sham-koua, Manaarii; Vanaa, Vincent; Lo, Cedrik; Cabral, Philippe.
Individual Pinctada margaritifera molluscs were collected from the Takapoto atoll (Tuamotu Archipelago, French Polynesia) and used to produce ten first generation full-sib families in a hatchery system, following artificial breeding protocols. After three years of culture, these progenies were transferred to Rangiroa atoll (Tuamotu Archipelago, French Polynesia) and tested for their potential as graft donors. A large-scale grafting experiment of 1500 grafts was conducted, in which a single professional grafter used ten individual donor oysters from each of the ten families, grafting 15 recipient oysters from each donor. The recipient oysters were all obtained from wild spat collection in Ahe (Tuamotu Archipelago, French Polynesia). After 18 months of...
Tipo: Text
Palavras-chave: Cultured pearl oyster; Pearl quality; Progeny effects; Heritability; Genetic selection; Pinctada margaritifera; French Polynesia.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00152/26310/24380.pdf

Whole transcriptome sequencing and biomineralization gene architecture associated with cultured pearl quality traits in the pearl oyster, Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Le Luyer, Jeremy; Auffret, Pauline; Quillien, Virgile; Leclerc, Nicolas; Reisser, Celine; Vidal-dupiol, Jeremie; Ky, Chin-long.
Background Cultured pearls are unique gems produced by living organisms, mainly molluscs of the  Pinctada genus, through the biomineralization properties of pearl sac tissue.  Improvement of P. margaritifera pearl quality is one of the biggest challenges that  Polynesian research has faced to date. To achieve this goal, a better understanding of the  complex mechanisms related to nacre and pearl formation is essential and can now be  approached through the use of massive parallel sequencing technologies. The aim of this  study was to use RNA-seq to compare whole transcriptome expression of pearl sacs that  had producing pearls with high and low quality. For this purpose, a comprehensive  reference transcriptome of P. margaritifera was built based on...
Tipo: Text
Palavras-chave: Pinctada margaritifera; Biomineralization; Pearl quality; Pearl sac; RNA-seq.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00475/58695/61207.pdf

Molecular Pathways and Pigments Underlying the Colors of the Pearl Oyster Pinctada margaritifera var. cumingii (Linnaeus 1758)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Stenger, Pierre-louis; Ky, Chin-long; Reisser, Celine; Duboisset, Julien; Dicko, Hamadou; Durand, Patrick; Quintric, Laure; Planes, Serge; Vidal-dupiol, Jeremie.
The shell color of the Mollusca has attracted naturalists and collectors for hundreds of years, while the molecular pathways regulating pigment production and the pigments themselves remain poorly described. In this study, our aim was to identify the main pigments and their molecular pathways in the pearl oyster Pinctada margaritifera—the species displaying the broadest range of colors. Three inner shell colors were investigated—red, yellow, and green. To maximize phenotypic homogeneity, a controlled population approach combined with common garden conditioning was used. Comparative analysis of transcriptomes (RNA-seq) of P. margaritifera with different shell colors revealed the central role of the heme pathway, which is involved in the production of red...
Tipo: Text
Palavras-chave: Pinctada margaritifera; Color; Pigment; Transcriptomics; Raman spectroscopy.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00685/79671/82446.pdf

Fitness of early life stages in F1 interspecific hybrids between Dicentrarchus labrax and D. punctatus
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Vergnet, Alain; Molinari, Nicolas; Fauvel, Christian; Bonhomme, Francois.
Inter- and intraspecific crossbreeding experiments were conducted to evaluate the aquaculture potential of hybrids in the genus Dicentrarchus, focusing on fertilisation and hatching success. The experimental design consisted of 24 controlled crosses in which individual and pooled fertilisations were made between wild D. labrax individuals (8 dams and 5 sires originating from West Mediterranean and Atlantic populations) and wild D. punctatus (6 sires). Three experiments were successively performed: (1) dams from the Mediterranean population, individually considered to have good egg quality, (2) dams from Mediterranean population, individually considered to have poor egg quality and (3) crossbreeding using pooled eggs from the Atlantic population. In each...
Tipo: Text
Palavras-chave: Sea bass; Hybrid progeny; Offspring quality; Fitness; Dicentrarchus labrax; Dicentrarchus punctatus.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00078/18881/16451.pdf

Shell Growth Performance of Hatchery Produced Pinctada margaritifera: Family Effect and Relation with Cultured Pearl Weight
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Le Moullac, Gilles.
Size is the most important and valuable quality trait of cultured pearls produced by the black-lipped pearl oyster, Pinctada margaritifera. In French Polynesia, several breeding programmes have been started that aim to improve this size trait, which is highly related to shell growth rate in both recipient and donor oysters. Shell growth rate dictates the time of grafting, size of implanted nuclei and bio-mineralisation potential of the mantle and pearl sac. We assessed shell growth rate through routine digital shell biometric analysis on 22 hatchery families produced between 2005 and 2008. These included full-sib families and half-sib families derived from polyandry (one dam crossed with two or more sires). Results showed that: 1) a significant family...
Tipo: Text
Palavras-chave: Pinctada margaritifera; Pearl oyster; Hatchery-produced families; Growth performance; Shell diameter; Cultured pearl size.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00387/49810/50384.pdf

Optimal age of the donor graft tissue in relation to cultured pearl phenotypes in the mollusc, Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Blay, Carole; Planes, Serge; Ky, Chin-long.
Ageing is defined as the progressive decline in tissue and organ functions over time. This study aims to evaluate the ageing effect on cultured pearl quality phenotypes (including size and quality traits) in the graft-recipient animal model: Pinctada margaritifera. For this, eight uniform grafting experiments were designed using two hatchery-produced pearl oyster families as donors, which were followed through time, between 7 and 30 months in age. For each age category, 20 donors were studied for each culture site giving a total of 2400 grafted oysters. Several phenotypic measurements were made: 1) donor family growth performance from shell size records, 2) pearl size and corresponding quality traits, and 3) expression of some genes related to...
Tipo: Text
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00444/55593/57220.pdf

Microbiome of the Black-Lipped Pearl Oyster Pinctada margaritifera, a Multi-Tissue Description With Functional Profiling
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Dubé, Caroline; Ky, Chin-long; Planes, Serge.
Elucidating the role of prokaryotic symbionts in mediating host physiology has emerged as an important area of research. Since oysters are the world’s most heavily cultivated bivalve molluscs, numerous studies have applied molecular techniques to understand the taxonomic and functional diversity of their associated bacteria. Here, we expand on this research by assessing the composition and putative functional profiles of prokaryotic communities from different organs/compartments of the black-lipped pearl oyster Pinctada margaritifera, a commercially important shellfish valued for cultured pearl production in the Pacific region. Seven tissues, in addition to mucous secretions, were targeted from P. margaritifera individuals: the gill, gonad, byssus gland,...
Tipo: Text
Palavras-chave: Pearl oyster; Microbiome; Tissue-specific bacterial communities; 16S rRNA gene sequencing; Functional profiling prediction.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00505/61670/65589.pdf

Is generalization of exhaled CO assessment in primary care helpful for early diagnosis of COPD?
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Molinari, Nicolas; Abou-badra, Mathieu; Marin, Gregory; Ky, Chin-long; Amador, Noemi; Gamez, Anne Sophie; Vachier, Isabelle; Bourdin, Arnaud.
Background: COPD is largely under-diagnosed and once diagnosed usually at a late stage. Early diagnosis is thoroughly recommended but most attempts failed as the disease is marginally known and screening marginally accepted. It is a rare cause of concern in primary care and spirometry is not very common. Exhaled carbon monoxide (eCO) is a 5-seconds easy-to-use device dedicated to monitor cigarette smoke consumption. We aimed to assess whether systematic eCO measurement in primary care is a useful tool to improve acceptance for early COPD diagnosis. Methods: This was a two-center randomized controlled trial enrolling 410 patients between March and May, 2013. Whatever was the reason of attendance to the clinic, all adults were proposed to measure eCO during...
Tipo: Text
Palavras-chave: COPD; Screening; Exhaled CO; Smoking; Behaviour.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00270/38142/36280.pdf

Variation in cultured pearl quality traits in relation to position of saibo cutting on the mantle of black-lipped pearl oyster Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Nakasai, Seiji; Parrad, Sophie; Broustal, Floriane; Devaux, Dominique; Louis, Patricia.
Cultured pearl production from Pinctada margaritifera uses the biomineralization capacities of the mantle graft, the saibo, which is usually obtained from only the middle mantle section of the donor oyster. To evaluate the potential for using other parts of the mantle, this study explores and describes the cultured pearl quality traits, pearl size, shape, surface defects and colour parameters obtained with saibo from the entire length of the mantle, comprising the four following sections: 1) posterior, 2) connection with the gills, 3) middle, (the section usually used commercially), and 4) anterior. Rates of nucleus retention and oyster mortality were also recorded and compared between sections. For this, two experimental grafts were designed and conducted...
Tipo: Text
Palavras-chave: Pinctada margaritifera; Pearl oyster; Pearl quality traits; Saibo position; Mantle tissue; Phenotype.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00439/55030/56461.pdf

Indirect improvement of pearl grade and shape in farmed Pinctada margaritifera by donor "oyster" selection for green pearls
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Blay, Carole; Sham-koua, Manaarii; Lo, Cédrik; Cabral, Philippe.
The top aquaculture species in French Polynesia is Pinctada margaritifera, a mollusc grown for the production of a unique gem: the black pearl. One of the challenges facing the pearl farming industry is to "produce less but better pearls" through genetic improvement. An experimental hatchery system was used to generate full-sib families to be tested for their potential as donor "oysters". A large-scale grafting experiments was done and seven cultured pearl quality traits: grade, surface defects, lustre, darkness level, visual colour categories, circles and shape categories were recorded. Our results revealed, for the first time, significant phenotypic relationships between these quality traits. The grade A cultured pearl class had the largest proportion of...
Tipo: Text
Palavras-chave: Pearl "oyster"; Pinctada margaritifera; Donor "oyster"; Cultured pearl quality; Pearl grade; Pearl colour; Pearl shape; Selective breeding.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00190/30130/28592.pdf

Quality Trait Signature at Archipelago Scale of the Cultured Pearls Produced by the Black-Lipped Pearl Oyster (Pinctada margaritifera Var. cumingi) in French Polynesia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Okura, Retsu; Nakasai, Seiji; Devaux, Dominique.
In French Polynesia, the aquaculture of Pinctada margaritifera (Linnaeus, 1758) covers a large maritime exploitation area, spread over nearly 20 degrees latitude and longitude, with numerous pearl farms located in three archipelagos (Gambier, Society, and Tuamotu). As these archipelagos have specific seasonal temperature patterns each year, pearl oysters are subject to disparate and contrasting environmental regimes. This study aimed to examine the specificity of commercial pearl quality traits (n ¼ 2,236 samples) at the archipelago scale, in such a way as to provide preliminary data to design the most appropriate strategy for the distribution of hatchery-produced phenotypes. A large and standardized grafting experiment using the same donor phenotype was...
Tipo: Text
Palavras-chave: Pearl oyster; Pinctada margaritifera; Large-scale geographic effects; Pearl color; Pearl grade; Pearl shape; Pearl size.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00357/46798/46665.pdf

Effect of electrolysis treatment on the biomineralization capacities of pearl oyster Pinctada margaritifera juveniles
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Latchere, Oihana; Fievet, Julie; Lo, Cedrik; Schneider, Denis; Dieu, Stephanie; Cabral, Philippe; Belliard, Corinne; Ky, Chin-long; Gueguen, Yannick; Saulnier, Denis.
The present study investigated the effect of electrolysis on the biomineralization capacities of juveniles of the mollusk Pinctada margaritifera for the first time. Size-selected individuals from two groups, “Medium” and “Large”, from a multi-parental family produced in a hatchery system were subjected to electrolysis under a low voltage current over a nine-week experimental period. The growth of the juveniles was individually monitored and assessed weekly by wet weight and shell height measurements. At the end of the experiment, mantle tissue was sampled for biomineralization-related gene expression analysis. Electrolysis significantly increased pearl oyster growth in terms of shell height and wet weight for Large juveniles from the 5th and the 2nd week,...
Tipo: Text
Palavras-chave: Pearl oyster; Pinctada margaritifera; Electrolysis; Biomineralization; Gene expression.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00343/45410/44924.pdf

Donor and recipient contribution to phenotypic traits and the expression of biomineralisation genes in the pearl oyster model Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Blay, Carole; Planes, Serge; Ky, Chin-long.
Grafting associates two distinct genotypes, each of which maintains its own genetic identity throughout the life of the grafted organism. Grafting technology is well documented in the plant kingdom, but much less so in animals. The pearl oyster, Pinctada margaritifera, produces valuable pearls as a result of the biomineralisation process of a mantle graft from a donor inserted together with a nucleus into the gonad of a recipient oyster. To explore the respective roles of donor and recipient in pearl formation, a uniform experimental graft was designed using donor and recipient oysters monitored for their growth traits. At the same time, phenotypic parameters corresponding to pearl size and quality traits were recorded. Phenotypic interaction analysis...
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00387/49809/50382.pdf

Age-dependence of cultured pearl grade and colour in the black-lipped pearl oyster Pinctada margaritifera
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Demmer, Jonathan; Blay, Carole; Lo, Cedrik.
Pinctada margaritifera is an economically important marine bivalve species for cultured pearl production in French Polynesian aquaculture. In order to evaluate the influence of donor oyster age on pearl quality traits, experiments were conducted over 6 years using both grafts and surgreffe operations. At harvest, 6 pearl quality traits were recorded and compared: surface defects, luster, grade, darkness level, and visual color. Analyzing the quality traits of pearls harvested in the initial graft process and those of pearls obtained from surgreffe experiments allowed a comparison of the influence of pearl sac cells originating from the initial mantle graft, which aged together with their recipient oysters. The results demonstrated a significant decrease...
Tipo: Text
Palavras-chave: Pearl oyster age; Pinctada margaritifera; Surgreffe; Pearl grade; Pearl colour.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00283/39472/37953.pdf

Donor effect on cultured pearl nacre development and shell matrix gene expression in Pinctada margaritifera reared in different field sites
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Broustal, Floriane; Koua, Manaarii Sham; Quillien, Virgile; Beliaeff, Benoit.
Understanding the respective roles played by donor and recipient pearl oysters in pearl quality determination in relation to the environment is a challenge for the pearl industry. In most Pinctada species, pearl size is mainly related to recipient oyster growth performance, but also relies to some extent on the biomineralisation activity of the pearl sac, a tissue that originates from the donor oyster mantle. We examined donor effect on pearl size in response to culture in the lagoons on Arutua and Apataki atolls. Overall nacre weight and thickness were greater in Arutua than in Apataki, but sensitivity to the environment differed between donors. Some donors were associated with significantly heavier and thicker nacre in Arutua (I group), while others had...
Tipo: Text
Palavras-chave: Biomineralisation; Environment; Pearl oyster; Pearl size; Pinctada margaritifera.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00425/53699/54610.pdf

Polynesian pearls
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Devaux, Dominique.
Black-lip pearl oyster culture in French Polynesia is still based on natural spat collection from wild stocks, but new developments in hatchery technology and selective breeding are bringing substantive change to the sector.
Tipo: Text
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00341/45252/44760.pdf

Macro-geographical differences influenced by family-based expression on cultured pearl grade, shape and colour in the black-lip "pearl oyster" Pinctada margaritifera: a preliminary bi-local case study in French Polynesia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Blay, Carole; Aiho, Vaite; Cabral, Philippe; Le Moullac, Gilles; Lo, Cedrik.
In French Polynesia, the aquaculture of P. margaritifera is carried out in numerous grow-out sites, located over three archipelagos (Gambier, Society and Tuamotu). To evaluate the impact of macro-geographical effects of these growing sites on pearl quality traits, five hatcheries produced families were used as homogeneous donor oysters in an experimental graft. The molluscs were then reared in two commercial locations: Tahaa island (Society) and Rangiroa atoll (Tuamotu). At harvest, eight pearl quality traits were recorded and compared: surface defects, lustre, grade, circles, shape categories, darkness level, body and secondary colour and visual colour categories. Overall inter-site comparison revealed that: 1) all traits were affected by grow-out...
Tipo: Text
Palavras-chave: Pinctada margaritifera; Cultured pearl quality; Grow-out location; Environment.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00276/38687/37186.pdf

Mono- and polychromatic inner shell phenotype diversity in Pinctada margaritifera donor pearl oysters and its relation with cultured pearl colour
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ky, Chin-long; Lo, Cedrik; Planes, Serge.
The pearl oyster Pinctada margaritifera has the specific ability to produce pearls with the widest range of colours among all pearl oyster species. This pearl colour diversity originates from the mantle biomineralising tissue (graft) of the donor oyster, which is originally responsible for the variety of colours of the inner shell surface. This study aimed to: 1) assess the geographic distribution and establish a first stocklist of the colourful oyster phenotypes used as donors in French Polynesia, and 2) investigate the phenotypic relation between inner shell colouration and the corresponding colour of harvested pearls. With the support of a pearl farmers' network, we investigated the different donor phenotype frequencies among five collection sites (Ahe,...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00354/46474/46249.pdf

Culture site dependence on pearl size realization in Pinctada margaritifera in relation to recipient oyster growth and mantle graft biomineralization gene expression using the same donor phenotype
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Le Pabic, Lore; Parrad, Sophie; Sham Koua, Manaarii; Nakasai, Seiji; Saulnier, Denis; Devaux, Dominique; Ky, Chin-long.
Size is the most important and valuable quality of the cultured black-lip pearl, Pinctada margaritifera. As this pearl aquaculture is carried out at numerous grow-out sites, this study analyzes the environmental influence on pearl size parameters (nacre weight and thickness) in relation to the recipient oyster biometric parameters (shell thickness, height, width, and oyster weight) at harvest time. Toward this end, an experimental graft was designed by using a homogeneous donor oyster phenotype. The recipient oysters were randomly and equally transferred and reared in five commercial and contrasting grow-out locations. Overall inter-site comparisons revealed that the cultured pearl size (N=2168) and the biometric parameters of the recipient oysters were...
Tipo: Text
Palavras-chave: Pinctada margaritifera; Cultured pearl size; Donor oysters; Recipient oysters; Gene expression; Environmental influences.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00321/43191/42738.pdf