Consulta: creatorFacets:"Oliveira,Claudio"
Registros recuperados: 70
Data/hora: 09/06/2024 16:58:11

Discrimination of Shark species by simple PCR of 5S rDNA repeats
Provedor de dados: Genetics and Molecular Biology
Autores: Pinhal,Danillo; Gadig,Otto BF; Wasko,Adriane P; Oliveira,Claudio; Ron,Ernesto; Foresti,Fausto; Martins,Cesar.
Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chondrichthyes; PCR; Species identification; 5S rDNA; Sharks.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200033

Phylogenetic analysis of the order Pleuronectiformes (Teleostei) based on sequences of 12S and 16S mitochondrial genes
Provedor de dados: Genetics and Molecular Biology
Autores: Azevedo,Marisa F.C.; Oliveira,Claudio; Pardo,Belén G.; Martínez,Paulino; Foresti,Fausto.
The fish order Pleuronectiformes, composed of 14 families, has two suborders: Psettodoidei (with one family) and Pleuronectoidei (with thirteen families). The relationships among families of Pleuronectoidei and among the genera of their families have extensively been debated and a consensus has not yet been reached. In the present study, partial sequences of the 12S and 16S mitochondrial rRNA genes were obtained from 19 species belonging to the families Achiridae, Bothidae, Cynoglossidae, Paralichthyidae, Pleuronectidae, Scophthalmidae, and Soleidae. Additional sequences of 42 pleuronectiform species were obtained from GenBank. Phylogenetic analyses were conducted by the methods of maximum-parsimony, maximum-likelihood and Bayesian inference. Our results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogeny; Molecular systematics; Mitochondrial DNA; Fish evolution; Flatfishes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200023

Cytogenetic analysis of five species of the subfamily Corydoradinae (Teleostei: Siluriformes: Callichthyidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Shimabukuro-Dias,Cristiane Kioko; Oliveira,Claudio; Foresti,Fausto.
In the present study, five callichthyid species belonging to the subfamily Corydoradinae were karyotyped: three species of Aspidoras and two of Corydoras. The three species of Aspidoras had the same diploid number, 2n = 46 chromosomes, similar karyotypic formulae, with most chromosomes metacentric or submetacentric, single interstitial Ag-NORs and C-band positive segments mainly found in the centromeric position. The comparative analysis of cytogenetic data available for the genus Aspidoras and other species of Corydoradinae suggest that several events of centric fusion occurred in the origin of the species of Aspidoras. The two analyzed species of Corydoras showed high diploid numbers, 2n = 74 in C. sodalis and 2n = 90 in C. britskii. While C. sodalis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish cytogenetic; Karyotype; NOR; C-banding; Karyotypic evolution.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400014

Mitochondrial DNA variation in wild populations of Leporinus elongatus from the Paraná River basin
Provedor de dados: Genetics and Molecular Biology
Autores: Martins,Cesar; Wasko,Adriane P.; Oliveira,Claudio; Foresti,Fausto.
Leporinus elongatus, a fish species widely distributed throughout the Paraná River basin in South America, is an important fishery resource and a valuable species in aquaculture programs. Despite its great economic importance, several wild populations have been suffering a drastic reduction. The comprehension of its population structure represents an important step for the conservation of these organisms in natural environments, and also for the selection of wild stocks to be used in hatchery programs. In order to understand the genetic-population structure of L. elongatus, D-loop mitochondrial DNA analyses were applied in six wild populations of the species. The results were used to estimate the levels of within and among population genetic variability....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; D-loop; Genetic variability; Leporinus elongatus; MtDNA.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100006

A phylogenetic analysis of Brycon and Henochilus (Characiformes, Characidae, Bryconinae) based on the mitochondrial gene 16S rRNA
Provedor de dados: Genetics and Molecular Biology
Autores: Hilsdorf,Silva; Oliveira,Claudio; Lima,Flávio César Thadeo de; Matsumoto,Cristianne Kayoko.
The genus Brycon, the largest subunit of the Bryconinae, has 42 valid species distributed from southern Mexico to the La Plata River in Argentina. Henochilus is a monotypic genus, comprising a single species (H. wheatlandii) found in the upper Rio Doce basin. In the present study, partial sequences of the mitochondrial gene 16S were obtained for fifteen species of Brycon and for Henochilus wheatlandii. The results showed that the genus Brycon is paraphyletic, since Henochilus is the sister-group of B. ferox and B. insignis. The most basal species analyzed were the trans-Andean species B. henni, B. petrosus, and B. chagrensis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogeny; Molecular systematics; Mitochondrial DNA; Fish evolution; Systematic.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200034

Analysis of the synaptonemal complex of the nine-banded armadillo, Dasypus novemcinctus
Provedor de dados: Genetics and Molecular Biology
Autores: Scavone,Márcia Denise da Paixão; Oliveira,Claudio; Bagagli,Eduardo; Foresti,Fausto.
The synaptonemal complex (SC) of three specimens of the nine-banded armadillo (Dasypus novemcinctus) was analyzed. Thirty-two bivalents (2n = 64) were observed, 31 of them being autosomes and one an XY sexual bivalent. Chromosome synapsis processes and nucleolus structure changes were analyzed in zygotene and pachytene cells, allowing a detailed description of the beginning of meiotic prophase in this species. There was complete synapsis of X and Y chromosomes. Some abnormalities in SC were observed in cells during zygotene and at the beginning of pachytene, but not in cells in the middle and late pachytene, suggesting the occurrence of synaptic adjustments in their SC.
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300020

Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelensis (Characiformes, Characidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Nirchio,Mauro; Oliveira,Claudio; Ferreira,Irani Alves; Granado,Angel; Ron,Ernesto.
The karyotype and chromosomal characteristics of the characid fish Triportheus venezuelensis were investigated using differential staining techniques (C-banding, Ag-NOR staining) and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. The diploid chromosome number (2n = 52), karyotype composition and sex chromosome determination system of the ZZ/ZW type were the same as previously described in other species of the genus Triportheus. However, extensive variation regarding nucleolus organizer regions (NOR) different from other species was observed. 18S rDNA sequences were distributed on nine chromosome pairs, but the number of chromosomes with Ag-NORs was usually lower, reaching a maximum of four chromosomes. When sequential staining experiments...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 18S rDNA; Ag-NORs; C-band; FISH; Fish cytogenetics; Sex chromosomes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100007

Nucleotide sequence, genomic organization and chromosome localization of 5S rDNA in two species of Curimatidae (Teleostei, Characiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Lessandra Viviane de Rosa; Foresti,Fausto; Wasko,Adriane Pinto; Oliveira,Claudio; Martins,Cesar.
The 5S ribosomal DNA (5S rDNA) of higher eukaryotes is organized in repeat units of tandem arrays composed of a 5S rDNA coding region, conserved even among non-related taxa, and a variable non-transcribed spacer sequence (NTS). To contribute to knowledge on the organization and evolution of vertebrate 5S rDNA we used PCR, nucleotide sequencing, Southern blot hybridization and chromosome fluorescence in situ hybridization (FISH) to investigate 5S rDNA tandem repeats in the South American Curimatidae fish Steindachnerina insculpta and Cyphocharax modesta. 5S rDNA repeats of 180 base pairs (bp) from both species were PCR-generated and sequenced evidencing the shortest 5S rDNA monomer so far described in eukaryote species. Southern blotting revealed that both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyphocharax modesta; Steindachnerina insculpta; 5S rDNA; Chromosomes; FISH; Nucleotide sequence.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200009

Cytogenetic analysis of three sea catfish species (Teleostei, Siluriformes, Ariidae) with the first report of Ag-NOR in this fish family
Provedor de dados: Genetics and Molecular Biology
Autores: Nirchio,Mauro; Martinez,Emanuel Ricardo Monteiro; Foresti,Fausto; Oliveira,Claudio.
Despite their ecological and economical importance, fishes of the family Ariidae are still genetically and cytogenetically poorly studied. Among the 133 known species of ariids, only eight have been karyotyped. Cytogenetic analyses performed on Genidens barbus and Sciades herzbergii revealed that both species have 2n = 56 chromosomes and Cathorops aff. mapale has 2n = 52 chromosomes: Genidens barbus has 10 Metacentrics (M), 14 Submetacentrics (SM), 26 Subtelocentrics (ST), and 6 Acrocentrics (A), Sciades herzbergii has 14M, 20SM, 18ST and 4A, whereas Cathorops aff. mapale has 14M, 20SM, and 18ST. The nucleolus organizer regions (NORs) were found in a single chromosome pair on the short arm of a large-sized ST pair in Genidens barbus and on the short arm of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Ag-NORs; Chromosome evolution; Chromosome rearrangements; Fish.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200011

Description of a ZZ/ZW sex chromosome system in Thoracocharax cf. stellatus (Teleostei, Characiformes, Gasteropelecidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Margarida Lima; Oliveira,Claudio; Foresti,Fausto.
The family Gasteropelecidae is composed of three genera and eight species. This study shows that Thoracocharax cf. stellatus has 2n = 52 chromosomes for both sexes. The five males studied showed 8 metacentric, 16 submetacentric, 4 subtelocentric, and 24 acrocentric chromosomes; the seven females showed only one submetacentric chromosome, belonging to pair 11, and one extra acrocentric chromosome, smaller than all the other chromosomes, characterizing the presence of a ZZ/ZW sex chromosome system in this species. Nucleolus organizing regions (NORs) were detected on the short arms of the subtelocentric chromosome pair 13. Constitutive heterochromatin was identified at pericentromeric and terminal positions in almost all chromosomes. The W chromosome was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sex chromosomes; ZW; DNA content; Fish; Evolution.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300008

R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Maistro,Edson Luis; Foresti,Fausto; Oliveira,Claudio.
The absence of longitudinal bands in fish chromosomes has been associated with technical problems in chromosome preparations or the absence of a structural compartmentalization in the fish genome. In the present study, a R-banding pattern was obtained using a replication banding technique by in vivo treatment with 5-bromodeoxyuridine (5-BrdU). G-banding patterns were obtained after trypsin treatment and also after chromosome cleavage by in situ treatment with the restriction endonuclease BamHI. A similar G-banding pattern was also obtained after cleavage with the endonuclease HinfI. Presence of a resolute R- and G-banding patterns shows that Astyanax scabripinnis paranae chromosomes could present an isochore-like structure similar to that found in other...
Tipo: Info:eu-repo/semantics/article
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200011

Genetic variability of two populations of Pseudoplatystoma reticulatum from the Upper Paraguay River Basin
Provedor de dados: Genetics and Molecular Biology
Autores: Abreu,Marcia Matos de; Pereira,Luiz Henrique Garcia; Vila,Vilma Barretto; Foresti,Fausto; Oliveira,Claudio.
Catfishes of the genus Pseudoplatystoma are very important species due to both their high commercial value and their ecological role as voracious predators. They undertake lengthy migratory movements during their life-cycle, this including reproductive migration which occurs from October to December in the rainy season. In the present study, seven microsatellite loci were analyzed to access genetic variability in two samples of P. reticulatum from the Upper Paraguay Basin. The loci were highly polymorphic (mean = 7.28). According to all analysis, the two samples of P. reticulatum revealed pronounced genetic differentiation. Fst value was 0.2290, Rst value 0.1067 and AMOVA 22.90% (Fst) and 10.67% (Rst), all being highly significant (p &lt; 0.001). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Population genetics; Microsatellite; Homing; Population structure.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400032

Sympatric occurrence of two cytotypes of Astyanax scabripinnis (Characiformes, Characidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Maistro,Edson Luis; Oliveira,Claudio; Foresti,Fausto.
Among four specimens of Astyanax scabripinnis sympatrically collected from the Tamanduá stream (State of São Paulo, Brazil), three, named cytotype I, had 2n = 50 chromosomes (6M + 26SM + 4ST + 14A) and a fundamental number (FN) of 86, and one specimen, named cytotype II, had 2n = 48 chromosomes (6M + 28SM + 4ST + 10A) and FN = 86. Besides the difference in diploid number, the cytotypes showed a clear difference in the distribution of constitutive heterochromatin. One NOR-bearing chromosome pair was detected in both cytotypes and one specimen of cytotype I had multiple NORs (tree chromosome pairs). Robertsonian translocations and an increase or loss of heterochromatin are proposed to explain the karyotypic divergence observed. Some aspects related to the...
Tipo: Info:eu-repo/semantics/other
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200021

5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers
Provedor de dados: Genetics and Molecular Biology
Autores: Alves-Costa,Fernanda A.; Martins,Cesar; Matos,Fernanda Del Campos de; Foresti,Fausto; Oliveira,Claudio; Wasko,Adriane P.
In order to extend the genetic data on the Sciaenidae fish family, the present study had the purpose to characterize PCR-generated 5S rDNA repeats of twelve species of this group through PAGE (Polyacrylamide Gel Electrophoresis) analysis. The results showed the occurrence of at least two different 5S rDNA size classes in all the species. Moreover, 5S rDNA repeats of one of the studied species - Isopisthus parvipinnis - were cloned and subjected to nucleotide sequencing and Southern blot membrane hybridization analyses, which permitted to confirm the existence of two major 5S rDNA classes. Phylogenetic analysis based on the nucleotide sequences of different 5S rDNA repeats of I. parvipinnis lead to their separation into two major clusters. These results may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sciaenidae; Fish; Isopisthus parvipinnis; 5S rDNA; Molecular markers.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200025

Analysis of NOR distribution in cultivated and naturalized stocks of rainbow trout (Oncorhynchus mykiss)
Provedor de dados: Genetics and Molecular Biology
Autores: Porto-Foresti,Fábio; Oliveira,Claudio; Tabata,Yara A.; Rigolino,Marcos G.; Foresti,Fausto.
In salmonid species, nucleolar organizing regions (NORs) have frequently been found on a single chromosome pair. However, some other, smaller yet active NORs have also been described. In the present study, NORs were found to be located in different positions on the chromosomes of 12 rainbow trout samples (11 cultivated and one naturalized stocks). Three phenotypes were found: 1) NORs located on two submetacentric chromosomes, in a subterminal position on the short arm (AA); 2) NORs located on two submetacentric chromosomes, in a subterminal position on the long arm (BB); and 3) NORs located on two submetacentric chromosomes, one in a subterminal position on the short arm, and the other in a subterminal position on the long arm (AB). Phenotype AA was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NOR polymorphism; Fish cytogenetics; Rainbow trout.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400009

Nuclear DNA content determination in Characiformes fish (Teleostei, Ostariophysi) from the Neotropical region
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Margarida Lima; Oliveira,Claudio; Navarrete,Maria Clara; Froehlich,Otávio; Foresti,Fausto.
In the present study, nuclear DNA content was analyzed in 53 species of Characiformes fish from the Neotropical region. Diploid number ranged from 2n = 48 in Astyanax fasciatus, Gymnocorymbus ternetzi and Hyphessobrycon griemi to 2n = 102 in Potamorhina squamoralevis, with a modal number of 54 chromosomes. Nuclear DNA content ranged from 1.70 ± 0.04 pg of DNA per diploid nucleus in Acestrorhynchus pantaneiro to 3.94 ± 0.09 pg in Tetragonopterus chalceus. A general analysis showed a mean value of 2.9 pg of DNA per diploid nucleus. Very similar DNA content values were observed in the species of the family Cynodontidae which showed a variation of 3% between the two genera studied. Small variations were observed between populations of Gymnocorymbus ternetzi,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nuclear DNA; DNA content; Chromosome; Fish; Evolution.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100010

Evidence of an XX/XY sex chromosome system in the fish Dormitator maculatus (Teleostei, Eleotrididae)
Provedor de dados: Genetics and Molecular Biology
Autores: Oliveira,Claudio; Toledo,Lurdes Foresti de Almeida.
The fish Dormitator maculatus has a chromosomes number of 2n = 46, females having a karyotype of 14 M, 28 SM, 2 ST and 2A and males 13 M, 28 SM, 3 ST and 2A. The presence of a heteromorphic pair in the males and a corresponding homomorphic pair in the females suggest the occurrence of an XX/XY sex chromosome system in D. maculatus. The putative X chromosome has a pericentromeric C-band positive segment and the putative Y chromosome a C-band positive short arm.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish cytogenetics; Karyotypes; Ag-NORs; C-band; Sex chromosomes.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400013

Cytogenetic analysis of three catfish species of the family Pseudopimelodidae (Teleostei, Siluriformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Martinez,Emanuel Ricardo Monteiro; Nirchio,Mauro; Granado,Angel; Foresti,Fausto; Oliveira,Claudio.
Cytogenetic analyses performed in Cephalosilurus apurensis, Microglanis aff. cottoides and Pseudopimelodus bufonius revealed that the three species have 2n = 54 chromosomes: C. apurensis presented six metacentric (M), 28 submetacentric (SM), 14 subtelolocentric (ST), and six acrocentric (A) chromosomes, while M. aff. cottoides showed 10M, 32SM, 10ST and 2A, and P. bufonius had 12M, 30SM and 12ST. The nucleolus organizer regions (NORs) were present on the short arm of a middle-sized ST pair, identified as pair 19, in C. apurensis NORs were found on the short arm of a middle-sized ST (pair 23) and on the long arm of a middle-sized ST (pair 22) in M. aff. cotttoides and on the short arm of three middle-sized ST pairs, identified as pairs 9, 10 and 11, in P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Chromosomes; C-banding; Ag-NOR; Fish.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400015

Karyotypic conservatism in samples of Characidium cf. zebra (Teleostei, Characiformes, Crenuchidae): physical mapping of ribosomal genes and natural triploidy
Provedor de dados: Genetics and Molecular Biology
Autores: Pansonato-Alves,José Carlos; Oliveira,Claudio; Foresti,Fausto.
Basic and molecular cytogenetic analyses were performed in specimens of Characidium cf. zebra from five collection sites located throughout the Tietê, Paranapanema and Paraguay river basins. The diploid number in specimens from all samples was 2n = 50 with a karyotype composed of 32 metacentric and 18 submetacentric chromosomes in both males and females. Constitutive heterochromatin was present at the centromeric regions of all chromosomes and pair 23, had additional interstitial heterochromatic blocks on its long arms. The nucleolar organizer regions (NORs) were located on the long arms of pair 23, while the 5S rDNA sites were detected in different chromosomes among the studied samples. One specimen from the Alambari river was a natural triploid and had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characidium; Heterochromatin; 18S rDNA; 5S rDNA; Natural triploidy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200007

Supernumerary chromosomes in the pufferfish Sphoeroides spengleri: first occurrence in marine Teleostean Tetraodontiformes fish
Provedor de dados: Genetics and Molecular Biology
Autores: Alves,Anderson Luis; Porto-Foresti,Fábio; Oliveira,Claudio; Foresti,Fausto.
Cytogenetic analyses carried out in eight specimens of Sphoeroides spengleri revealed the presence of 2n = 46 chromosomes (20 M/SM and 26 ST/A). Besides the standard karyotypical set, the presence of B microchromosomes was observed in two individuals, ranging from 0 to 2 microchromosomes per cell. A karyotype composed by 2n = 46 chromosomes with occurrence of M and SM chromosomes is considered basal for the species from the clade comprising the families Tetraodontidae, Balistidae, and Diodontidae, although it represents a derived condition for the order Tetraodontiformes, whose basal karyotype would be composed by 2n = 48 acrocentric chromosomes. The occurrence of B microchromosomes in marine Tetraodontiformes fish was not known, and this represents the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B chromosome; Pufferfish; Sphoeroides spengleri.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200014

Nuclear DNA content in 20 species of Siluriformes (Teleostei: Ostariophysi) from the Neotropical region
Provedor de dados: Genetics and Molecular Biology
Autores: Fenerich,Paulo César; Foresti,Fausto; Oliveira,Claudio.
In the present study, 20 species of Siluriformes fish were analyzed in order to determine their nuclear DNA content and compare these data with their diploid number. In addition, the extension and importance of the changes that occurred during the process of diversification in the group of Neotropical freshwater catfish were investigated. The only species studied of the family Doradidae, Rhinodoras d'orbignyi (2n = 58), presented 3.46 ± 0.13 pg of DNA. Among the species of the family Heptapteridae, the values of nuclear DNA content and the diploid numbers ranged from 1.13 ± 0.09 pg of DNA in Pimelodella sp. (2n = 46) to 2.38 ± 0.07 pg of DNA in Imparfinis mirini (2n = 58). The family Loricariidae showed the widest variation in diploid number and nuclear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nuclear DNA; DNA content; Chromosome; Fish; Evolution.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300008

Scattered organization of the histone multigene family and transposable elements in Synbranchus
Provedor de dados: Genetics and Molecular Biology
Autores: Utsunomia,Ricardo; Pansonato-Alves,José Carlos; Scacchetti,Priscilla Cardim; Oliveira,Claudio; Foresti,Fausto.
The fish species Synbranchus marmoratus is widely distributed throughout the Neotropical region and exhibits a significant karyotype differentiation. However, data concerning the organization and location of the repetitive DNA sequences in the genomes of these karyomorphs are still lacking. In this study we made a physical mapping of the H3 and H4 histone multigene family and the transposable elements Rex1 and Rex3 in the genome of three known S. marmoratus karyomorphs. The results indicated that both histone sequences seem to be linked with one another and are scattered all over the chromosomes of the complement, with a little compartmentalization in one acrocentric pair, which is different from observations in other fish groups. Likewise, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synbranchidae; FISH; Histone; Retrotransposon.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100007

A lethal effect associated with polymorphism of the NOR-bearing chromosomes in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: Genetics and Molecular Biology
Autores: Porto-Foresti,Fábio; Oliveira,Claudio; Gomes,Eduardo A.; Tabata,Yara A.; Rigolino,Marcos G.; Foresti,Fausto.
Cytogenetic analysis of a rainbow trout stock showed that the nucleolar organizing regions were located subterminally on the long arm of a submetacentric chromosome pair and occurred as a single chromosomal segment (phenotype N1) or as two chromosomal segments separated by a short euchromatic segment (phenotype N2). Cytogenetic analysis also showed that there were N1N1 and N1N2 individuals but no N2N2 individuals. Analysis of the different includedphenotypes incluted that the population was not in Hardy-Weinberg equilibrium (chi² = 19.333; p &lt; 0.01), and that a higher frequency of individuals had the N1N2 phenotype. Experimental crosses involving four males (two N1N1 and two N1N2) and four females (one N1N1 and three N1N2) yielded eight broods. There...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NOR polymorphism; Paracentric inversion; Fish cytogenetics; Rainbow trout.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100009

Nuclear DNA content of thirty species of Neotropical fishes
Provedor de dados: Genetics and Molecular Biology
Autores: Carvalho,Margarida Lima; Oliveira,Claudio; Foresti,Fausto.
The present paper reports nuclear DNA content in 30 Neotropical freshwater fish species and summarizes the data on other Neotropical species presented in the literature. Among Neotropical fishes, the nuclear DNA content ranges from 1.04 ± 0.09 pg/nucleus in Corydoras cf. simulatus (2n = 62) to 248.0 pg/nucleus in Lepidosiren paradoxa (2n = 38). A general analysis of the data obtained in the present study for each species showed that DNA measurements were practically constant at the individual level, while significant differences were observed among individuals of the same population. This observation was valid for all species analyzed and was more evident in those species that presented other karyotypic particularities such as sex chromosomes or...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100009

Comparative cytogenetic and morphological analysis of Astyanax scabripinnis paranae (Pisces, Characidae, Tetragonopterinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Maistro,Edson Luis; Oliveira,Claudio; Foresti,Fausto.
Cytogenetic and morphological studies were carried out on nine local populations of Astyanax scabripinnis paranae. All populations exhibited 2n = 50 chromosomes as well as conspicuous differences involving karyotype morphology, number and position of nucleolar organizer regions (NORs) and amount and/or locations of constitutive heterochromatin blocks. A quantitative study of the cytogenetic data showed that eight populations possessed different karyotypes. Morphological analyses based on nine measurements and two meristic parameters were effective in establishing clear identification of five populations. Comparative analysis of cytogenetic and morphological traits suggests that chromosomal changes have occurred at a more rapid rate than morphological...
Tipo: Info:eu-repo/semantics/article
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000200005

Karyological characterization of four Neotropical fish species of the genus Hisonotus (Teleostei, Loricariidae, Hypoptopomatinae) from distinct Brazilian river basins
Provedor de dados: Genetics and Molecular Biology
Autores: Andreata,Artur Antonio; Oliveira,Claudio; Foresti,Fausto.
The karyotypes of four Hisonotus species (two provisionally-named species A and D, H. nigricauda, and H. leucofrenatus) were found to have the same diploid number of 2n = 54 and interstitial silver-staining nucleolus organizer regions (Ag-NORs) located on the long arm of the largest metacentric pair. The C-banding pattern appeared to be species-specific, with one group (H. nigricauda and the unnamed species A and D) being characterized by small amounts of positive C-banded segments and containing a sub-group (species A and D) identified by a large positive C-banded segment on a small metacentric chromosome pair. The second group contained different samples of H. leucofrenatus, characterized by a larger amount of C-band positive segments spread over several...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish cytogenetics; Karyotypes; Ag-NORs; C-band; Chromosome evolution.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100013

Identification and description of distinct B chromosomes in Cyphocharax modestus (Characiformes, Curimatidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Santos,Lessandra Viviane De Rosa; Foresti,Fausto; Martins,Cesar; Oliveira,Claudio; Wasko,Adriane Pinto.
Cytogenetic analyses were performed in Cyphocharax modestus, collected at Paranapanema River and Tietê River (São Paulo State, Brazil). A karyotype with 2n = 54 chromosomes was observed in the animals from both Brazilian freshwater river systems. One to four B chromosomes were also detected in individuals from the Paranapanema River, which represents the probable first report of more than a single supernumerary element in a species of the Curimatidae group. C-banding revealed centromeric and telomeric heterochromatin blocks in several chromosomes of the normal karyotype complement of C. modestus. Moreover, while some B chromosomes were characterized by the complete absence of C-bands, others were totally heterochromatic. Although there was a prevalence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B chromosomes; C-banding; Curimatidae; Cyphocharax modestus; Supernumerary chromosomes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200019

Karyotype description of five species of Trichomycterus (Teleostei: Siluriformes: Trichomycteridae)
Provedor de dados: Genetics and Molecular Biology
Autores: Sato,Luciana Ramos; Oliveira,Claudio; Foresti,Fausto.
Trichomycteridae is a family of small catfish which are widely distributed throughout Southern Central America and South America. The present study showed that the cis-Andean species Trichomycterus florensis, Trichomycterus sp. aff. Trichomycterus itatiyae, Trichomycterus reinhardti, Trichomycterus davisi and Trichomycterus auroguttatus had 2n = 54 chromosomes (42 metacentric, 10 submetacentric and 2 subtelocentric), with T. reinhardti, T. auroguttatus and T. sp. aff. T. itatiyae exhibiting only one chromosome pair with silver-stained nucleolus organizer regions (NORs). The cytogenetic data suggest the existence of at least two groups of species in the cis-Andean representatives of the genus Trichomycterus. In the first group the first metacentric pair is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome evolution; NOR banding; Fish.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100008

Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses
Provedor de dados: Genetics and Molecular Biology
Autores: Poletto,Andréia B.; Wasko,Adriane P.; Oliveira,Claudio; Azevedo,Alexandre; Carvalho,Robson F.; Silva,Maeli Dal Pai; Foresti,Fausto; Martins,Cesar.
Actin-encoding cDNAs of Nile tilapia (Oreochromis niloticus) were isolated by RT-PCR using total RNA samples of different tissues and further characterized by nucleotide sequencing and in silico amino acid (aa) sequence analysis. Comparisons among the actin gene sequences of O. niloticus and those of other species evidenced that the isolated genes present a high similarity to other fish and other vertebrate actin genes. The highest nucleotide resemblance was observed between O. niloticus and O. mossambicus a-actin and b-actin genes. Analysis of the predicted aa sequences revealed two distinct types of cytoplasmic actins, one cardiac muscle actin type and one skeletal muscle actin type that were expressed in different tissues of Nile tilapia. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actin; Expression pattern; Nile tilapia; Oreochromis niloticus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200031

Cytogenetic analysis of five Hypostomus species (Siluriformes, Loricariidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Martinez,Emanuel Ricardo Monteiro; Zawadzki,Claudio Henrique; Foresti,Fausto; Oliveira,Claudio.
In this work, we analyzed the karyotypes of five species. Hypostomus cf. heraldoi, from the Mogi-Guaçu River, had 2n = 72 chromosomes, with a nucleolar organizer region (NOR) in one chromosomal pair. Hypostomus regani, from the Mogi-Guaçu River had 2n = 72 chromosomes with NORs in two chromosomal pairs. Hypostomus sp., from the Mogi-Guaçu River basin, had 2n = 68 chromosomes, with NORs in two chromosomal pairs. Hypostomus aff. agna, from Cavalo Stream, had 2n = 74 chromosomes with NORs in two chromosomal pairs. Hypostomus cf. topavae, from Carrapato Stream, had 2n = 80 chromosomes, with NORs in two chromosomal pairs. Hypostomus species showed marked diversity in the karyotypic formula, which suggested the occurrence of several Robertsonian rearrangements...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomes; Evolution; Hypostominae; Neotropical fish; NOR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400006

Spermiogenesis and spermatozoa ultrastructure in five species of the Curimatidae with some considerations on spermatozoal ultrastructure in the Characiformes
Provedor de dados: Neotropical Ichthyology
Autores: Quagio-Grassiotto,Irani; Gameiro,Maria Carolina; Schneider,Tatiana; Malabarba,Luiz Roberto; Oliveira,Claudio.
Spermiogenesis in the curimatid species, Steindachnerina insculpta, Cyphocharax gillii, C. modestus, C. spilotus, and Potamorhina altamazonica, is characterized by lateral development of the flagellum, nuclear rotation, eccentric nuclear fossa formation, and chromatin compacted into thick fibers. These spermatozoa exhibit a spherical head containing a nucleus with the chromatin highly condensed into thick fibers with small electron-lucent areas, and no acrosome. The nuclear fossa is of the moderate type and eccentric and penetrated by the centriolar complex. The midpiece is small, has many elongate vesicles, and a short cytoplasmic channel. Mitochondria may be elongate, branched or C-shaped, and are separated from the initial segment of the axoneme by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Spermatid; Sperm; Ultrastructure.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252003000100004

Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
Provedor de dados: Neotropical Ichthyology
Autores: Souza-Shibatta,Lenice; Ferreira,Dhiego Gomes; Oliveira,Claudio; Almeida,Fernanda Simões de; Shibatta,Oscar Akio; Sofia,Silvia Helena.
Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian Eastern basin; Catfish; Enriched genomic library; Genetic marker; SSR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300581

Cytogenetic studies in three species of Lutjanus (Perciformes: Lutjanidae: Lutjaninae) from the Isla Margarita, Venezuela
Provedor de dados: Neotropical Ichthyology
Autores: Nirchio,Mauro; Rondón,Rodolfo; Oliveira,Claudio; Ferreira,Irani A.; Martins,Cesar; Pérez,Julio; Sola,Luciana; Rossi,Anna Rita.
In the present study, three species of Lutjaninae, Lutjanus analis, L. griseus and L. synagris, were analyzed by conventional Giemsa staining, C-banding and silver staining, to reveal active Nucleolus Organizer Regions (NORs). Fluorescent in situ hybridization (FISH) was also applied to establish the number and location of the ribosomal gene clusters (18S and 5S rRNA genes). Counts of diploid metaphasic cells revealed a diploid modal chromosome complement composed of 48 acrocentric chromosomes in both L. analis and L. griseus. Two cytotypes were observed in L. synagris: cytotype I, with 2n=48 acrocentric chromosomes, found in 19 specimens, and cytotype II, with 46 acrocentric chromosomes and one large metacentric, found in two specimens. The large...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Ribosomal genes; NOR polymorphism; C-banding; Robertsonian rearrangement.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000100012

Chromosome evolution in fishes: a new challenging proposal from Neotropical species
Provedor de dados: Neotropical Ichthyology
Autores: Nirchio,Mauro; Rossi,Anna Rita; Foresti,Fausto; Oliveira,Claudio.
We present a database containing cytogenetic data of Neotropical actinopterygian fishes from Venezuela obtained in a single laboratory for the first time. The results of this study include 103 species belonging to 74 genera assigned to 45 families and 17 out of the 40 teleost orders. In the group of marine fishes, the modal diploid number was 2n=48 represented in 60% of the studied species, while in the freshwater fish group the modal diploid complement was 2n=54, represented in 21.21 % of the studied species. The average number of chromosomes and the mean FN were statistically higher in freshwater fish than in marine fish. The degree of diversification and karyotype variation was also higher in freshwater fish in contrast to a more conserved cytogenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actinopterygii; Ancestral karyotype; Diploid number; Fundamental number.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400761

Populations analysis of the Brazilian Sharpnose Shark Rhizoprionodon lalandii (Chondrichthyes: Carcharhinidae) on the São Paulo coast, Southern Brazil: inferences from mt DNA sequences
Provedor de dados: Neotropical Ichthyology
Autores: Mendonça,Fernando Fernandes; Oliveira,Claudio; Gadig,Otto Bismarck Fazzano; Foresti,Fausto.
Sharks of the genus Rhizoprionodon can be considered some of the most important predators along the trophic coastal marine ecosystems and represent an important economic resource for the small-scale fisheries, especially on the Brazilian coastline. In order to analyze the population structure of the shark Rhizoprionodon lalandii of São Paulo, Southeastern coast of Brazil, levels of genetic diversity were identified by nucleotide sequence analyses of the mitochondrial DNA control region. The results obtained from this study present moderate values of haplotype diversity and low nucleotide diversity. Although the AMOVA tests (ΦST = 0.08394, P &lt; 0.01) had shown slightly differences among the studied samples, evidence for the occurrence of population...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: D-loop; Haplotypes; Mitochondrial control region; Shark conservation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000200012

DNA barcode of Parodontidae species from the La Plata river basin - applying new data to clarify taxonomic problems
Provedor de dados: Neotropical Ichthyology
Autores: Bellafronte,Elisangela; Mariguela,Tatiane Casagrande; Pereira,Luiz Henrique Garcia; Oliveira,Claudio; Moreira-Filho,Orlando.
In the past years, DNA barcoding has emerged as a quick, accurate and efficient tool to identify species. Considering the difficulty in identifying some Parodontidae species from the La Plata basin and the absence of molecular data for the group, we aimed to test the effectiveness of DNA barcoding and discuss the importance of using different approaches to solve taxonomic problems. Eight species were analyzed with partial sequences of Cytochrome c oxidase I. The mean intraspecific K2P genetic distance was 0.04% compared to 4.2% for mean interspecific K2P genetic distance. The analyses of distance showed two pairs of species with K2P genetic divergence lower than 2%, but enough to separate these species. Apareiodon sp. and A. ibitiensis, considered as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apareiodon; COI gene; Cytogenetics; Fish identification; Morphology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300497

Protocheirodon, a new genus of Characidae (Teleostei: Characiformes) with the redescription of the poorly known Protocheirodon pi
Provedor de dados: Neotropical Ichthyology
Autores: Vari,Richard P.; Melo,Bruno F.; Oliveira,Claudio.
ABSTRACT Protocheirodon, a new genus of the Characidae, is proposed to include a species previously assigned to Leptagoniates . This action is supported by molecular and morphological phylogenetic hypotheses that place Protocheirodon pi as the sister group of the remaining tribes in the Cheirodontinae versus the traditional assignment of the species to the Aphyocharacinae. The phylogenetic position of Protocheirodon is recognized as a new tribe, the Protocheirodontini. Protocheirodon pi , originally described based on a small number of specimens from a single location in the río Mamoré basin in the southwestern Amazon, is redescribed on the basis of larger series of specimens from the western and central portions of the Amazon basin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aphyocharacinae; Amazon basin; Cheirodontinae; Leptagoniates; Taxonomy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200207

Molecular phylogeny and biogeographic history of the Neotropical tribe Glandulocaudini (Characiformes: Characidae: Stevardiinae)
Provedor de dados: Neotropical Ichthyology
Autores: Camelier,Priscila; Menezes,Naércio Aquino; Costa-Silva,Guilherme José; Oliveira,Claudio.
ABSTRACT Although former studies on systematics and biogeography represent a progress on the knowledge of the tribe Glandulocaudini, none was grounded on molecular evidence. Thus, the first hypothesis of relationships for the tribe based on a multilocus analysis is presented, including all genera and most of the valid species. DNA sequences of Glandulocauda caerulea and Mimagoniates sylvicola were analyzed for the first time. A molecular clock analysis was used to estimate the origin of the Glandulocaudini and the approximate timing of cladogenetic events within the group. Glandulocaudini was recovered as monophyletic. No hypothesis recovered Glandulocauda as monophyletic, since G. melanopleura is sister to Lophiobrycon weitzmani while G. caerulea is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian Crystalline Shield; Coastal Drainages; Molecular Clock; Molecular Systematics; Multilocus Analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000100208

Comparative analysis of spermiogenesis and sperm ultrastructure in Callichthyidae (Teleostei: Ostariophysi: Siluriformes)
Provedor de dados: Neotropical Ichthyology
Autores: Spadella,Maria A.; Oliveira,Claudio; Quagio-Grassiotto,Irani.
In Corydoradinae, the presence of spermatids in the lumen of the testicular tubules together with spermatozoa suggests that spermatogenesis is of the semicystic type, whereas in Callichthyinae, sperm production occurs entirely within spermatocysts in the germinal epithelium, characterizing cystic spermatogenesis. Spermiogenesis in Callichthyinae is characterized by an initial lateral development of the flagellum, the presence of nuclear rotation to different degrees, an eccentric or medial formation of a nuclear fossa, formation of a cytoplasmic channel, and presence of centriolar migration, being more similar to type I spermiogenesis. In Corydoradinae, spermiogenesis is characterized by eccentric development of the flagellum, the absence of nuclear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catfish; Spermatid; Spermatozoa; Phylogeny; Morphology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252007000300014

Cytogenetic and molecular markers reveal the complexity of the genus Piabina Reinhardt, 1867 (Characiformes: Characidae)
Provedor de dados: Neotropical Ichthyology
Autores: Pazian,Marlon Felix; Pereira,Luiz Henrique Garcia; Shimabukuru-Dias,Cristiane Kioko; Oliveira,Claudio; Foresti,Fausto.
Cytogenetic and molecular analyses were carried out in fish representative of the genus Piabina. This study specifically involved the species P. argentea and P. anhembi collected from areas of the Paranapanema and Tietê River basins, Brazil. Our findings suggest that fish classified as Piabina argentea in the Paranapanema and Tietê Rivers may represent more than one species. The samples analyzed differed by cytogenetic particularities and molecular analyses using partial sequences of the genes COI and CytB as genetic markers revealed three distinct groups of P. argentea with genetic distances sufficient to support the conclusion that the three samples analyzed are three distinct taxonomic units.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA Barcoding; Freshwater fishes; Fish; MtDNA; 18S rDNA; 5S rDNA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000200008

The bony fishes (Teleostei) caught by industrial trawlers off the Brazilian North coast, with insights into its conservation
Provedor de dados: Neotropical Ichthyology
Autores: Marceniuk,Alexandre Pires; Rotundo,Matheus Marcos; Caires,Rodrigo Antunes; Cordeiro,Ana Patricia Barros; Wosiacki,Wolmar Benjamin; Oliveira,Claudio; Souza-Serra,Rayla Roberta Maganhães de; Romão-Júnior,João Gomes; Santos,Wagner César Rosa dos; Reis,Thayson da Silva; Muniz,Mairink Ribeiro; Cardoso,Gustavo Stabile; Ferrari,Stephen; Klautau,Alex Garcia Cavalleiro de Macedo; Montag,Luciano.
ABSTRACT The Brazilian North coast is one of the world’s most important shrimp fishery grounds, with a total area of approximately 223,000 km2. However, the available data on the diversity of fish caught by the region’s industrial trawler fleet are limited to the commercially-valuable species. This lacuna in the data on the region’s marine fish fauna is worrying, both for the management of stocks and the conservation of the local biodiversity. The present study was based on a comprehensive inventory of the teleost fishes captured by the industrial outrigger trawling operations off the North coast of Brazil. This inventory recorded 201 species belonging to 64 families and 20 orders, and revealed a unique fauna, characterized by 17 endemic species, and a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity; Bycatch; Fishes discarded; Inventory; Marine fish; Outrigger trawling.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000200205

A new Hyphessobrycon (Characiformes: Characidae) of the Hyphessobrycon heterorhabdus species-group from the lower Amazon basin, Brazil
Provedor de dados: Neotropical Ichthyology
Autores: Faria,Tiago C.; Guimarães,Karen L. A.; Rodrigues,Luís R. R.; Oliveira,Claudio; Lima,Flávio C.T.
ABSTRACT A new species of Hyphessobrycon belonging to the Hyphessobrycon heterorhabdus species-group from the lower rio Tapajós, state of Pará, Brazil, is described. The new species is allocated into the Hyphessobrycon heterorhabdus species-group due to its color pattern, composed by an anteriorly well-defined, horizontally elongated humeral blotch that becomes diffuse and blurred posteriorly, where it overlaps with a conspicuous midlateral dark stripe that becomes blurred towards the caudal peduncle and the presence, in living specimens, of a tricolored longitudinal pattern composed by a dorsal red or reddish longitudinal stripe, a middle iridescent, golden or silvery longitudinal stripe, and a more ventrally-lying longitudinal dark pattern composed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity; Cryptic Species; DNA Barcoding; Rio Amazonas; Rio Tapajós.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100207

New species of the Corumbataia cuestae group (Siluriformes: Loricariidae) from the Rio Tocantins basin, with comments on its phylogenetic relationships
Provedor de dados: Neotropical Ichthyology
Autores: Thimotheo,Mateus Giovanni Rocha; Benine,Ricardo Cardoso; Oliveira,Claudio; Silva,Gabriel de Souza da Costa e.
Abstract A new species of Corumbataia is described from Rio Maranhão, Rio Tocantins basin, central Brazil. The new species is distinguished from all congeners by the presence of a small, naked area on snout tip; by having the abdomen covered with small platelets forming a shield which reaches the lateral mid-ventral plates; by the anterior profile of the head rounded in dorsal view; by the lower lip not reaching the transversal line of the pectoral girdle; and by the presence of 28 or 29 vertebrae. High genetic divergence in mitochondrial cytochrome c oxidase subunit I (COI) further supports the validity of this new species. Our phylogenetic analysis shows a derived subclade in Corumbataia, herein named as the Corumbataia cuestae group, composed of the new...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytochrome C Oxidase; Diversity; Freshwater fishes; Hypoptopomatinae; Central Brazil.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000400207

Cytogenetic characterization of Rhomboplites aurorubens and Ocyurus chrysurus, two monotypic genera of Lutjaninae from Cubagua Island, Venezuela, with a review of the cytogenetics of Lutjanidae (Teleostei: Perciformes)
Provedor de dados: Neotropical Ichthyology
Autores: Nirchio,Mauro; Oliveira,Claudio; Ferreira,Daniela C.; Rondón,Rodolfo; Pérez,Julio E.; Hett,Anne Kathrin; Rossi,Anna Rita; Sola,Luciana.
Lutjanidae, commonly known as snappers, includes 105 species, grouped in four subfamilies. In spite of the high number of species and of its worldwide distribution, the family has been little investigated and the phylogenetic relationships among some of its genera and species are still cause for debate. Only a small number of the species has been cytogenetically analysed. This study reports the first description of the karyotype of Rhomboplites aurorubens as well as data concerning the distribution of the constitutive heterochromatin and the location of the 18S rRNA and the 5S rRNA genes. Specimens of Ocyurus chrysurus from Venezuela were also investigated for the same cytogenetic features. Both species have a 48 uniarmed karyotype, but R. aurorubens has a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-banding FISH; Karyotype NORs Ribosomal genes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400005

A new species of Tetragonopterus Cuvier, 1816 (Characiformes: Characidae: Tetragonopterinae) from the rio Jari, Amapá, northern Brazil
Provedor de dados: Neotropical Ichthyology
Autores: Melo,Bruno F; Benine,Ricardo C; Mariguela,Tatiane C; Oliveira,Claudio.
A new species of Tetragonopterus is described from the rio Jari, a tributary to the left margin of rio Amazonas, at the border between Amapá and Pará States, northern Brazil. It is morphologically diagnosed from the other species of the genus (T. argenteus, T. chalceus, and T. rarus new combination) by the lozenge-shaped spot on the caudal peduncle vs. rounded to square spot on the other species. Partial sequences of the mitochondrial gene Cytochrome Oxidase C subunit I, from representatives of all valid species of Tetragonopterus, including this new species, were analyzed. The obtained results revealed a significant genetic distance between the new species and its congeners. A discussion on the new combination, Tetragonopterus rarus, is also provided.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COI gene; Genetic distance; Mitochondrial DNA; Neotropical fish; Taxonomy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100002

Variation in male reproductive system characters in Corydoradinae (Loricarioidei: Callichthyidae) reflects the occurrence of different lineages in this subfamily
Provedor de dados: Neotropical Ichthyology
Autores: Spadella,Maria A.; Desan,Simone P.; Henriques,Teresa C. B. P. O.; Oliveira,Claudio.
ABSTRACT Callichthyidae comprises a well-corroborated monophyletic group divided into two subfamilies: Corydoradinae and Callichthyinae. A recent proposal, based on molecular data, suggests that Corydoradinae is composed by nine monophyletic lineages, possibly genera. The species pertaining to those lineages have extensive modification in the size of genome, including diploid, tetraploid and octoploid species. Considering the occurrence of these monophyletic lineages and that the variations in DNA content may imply in significant alterations on the structure of spermatozoa, this study analyzed the morphology of the male reproductive system and the morphometry of the head of the spermatozoa of representatives of the nine lineages of Corydoradinae, seeking...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monophyly; Morphometry; Spermatozoa; Variations in DNA.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100221

Species delimitation reveals an underestimated diversity of Andean catfishes of the family Astroblepidae (Teleostei: Siluriformes)
Provedor de dados: Neotropical Ichthyology
Autores: Ochoa,Luz E.; Melo,Bruno F.; García-Melo,Jorge E.; Maldonado-Ocampo,Javier A.; Souza,Camila S.; Albornoz-Garzón,Juan G.; Conde-Saldaña,Cristhian C.; Villa-Navarro,Francisco; Ortega-Lara,Armando; Oliveira,Claudio.
ABSTRACT Catfishes of the family Astroblepidae form a group composed by 82 valid species of the genus Astroblepus inhabiting high-gradient streams and rivers throughout tropical portions of the Andean Cordillera. Little has been advanced in the systematics and biodiversity of astroblepids other than an unpublished thesis, a single regional multilocus study and isolated species descriptions. Here, we examined 208 specimens of Astroblepus that apparently belong to 16 valid species from several piedmont rivers from northern Colombia to southern Peru. Using three single-locus approaches for species delimitation in combination with a species tree analysis estimated from three mitochondrial genes, we identified a total of 25 well-delimited lineages including...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Andes; Catfishes; Delimitation; Ostariophysi; Systematics; Taxonomy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000400205

Molecular and cytogenetic analyses of cryptic species within the Synbranchus marmoratus Bloch, 1795 (Synbranchiformes: Synbranchidae) grouping: species delimitations, karyotypic evolution and intraspecific diversification
Provedor de dados: Neotropical Ichthyology
Autores: Utsunomia,Ricardo; Pansonato-Alves,José C.; Costa-Silva,Guilherme J.; Mendonça,Fernando F.; Scacchetti,Priscilla C.; Oliveira,Claudio; Foresti,Fausto.
The fish species Synbranchus marmoratushas been reported to exist as a species complex due to high intraspecific karyotypic variability in spite of the difficulty or impossibility to distinguish them using morphological traits alone. The goal of this work was to use cytogenetic and molecular methods to determine the species delimitations and understand the karyoevolution ofS. marmoratususing samples collected from distinct Brazilian localities. Among the analyzed specimens, a large degree of cytogenetic variation related to diploid numbers and karyotype structure was observed, with karyotypes showing 2n=42, 44 and 46 chromosomes. In addition, using sequences of three mitochondrial genes, the phylogenetic relationships between every sample with a known...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal diversity; Fish cytogenetics; Karyotype evolution; Repetitive DNA; Species complex.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400903

Revision of Bairdiella (Sciaenidae: Perciformes) from the western South Atlantic, with insights into its diversity and biogeography
Provedor de dados: Neotropical Ichthyology
Autores: Marceniuk,Alexandre Pires; Molina,Eduardo Garcia; Caires,Rodrigo Antunes; Rotundo,Matheus Marcos; Wosiacki,Wolmar Benjamin; Oliveira,Claudio.
ABSTRACT The sciaenid genus Bairdiella comprises a group of relatively small fishes found in inshore waters and estuaries of the western Atlantic and eastern Pacific. Despite recent analyses of the phylogenetic relationships of Bairdiella, there has been no comprehensive revision of the alpha taxonomy of the species of the genus. Bairdiella ronchus from the western Atlantic, has a complex taxonomic history, with four junior synonyms recognized. Based on morphological and molecular evidence, this study indicates that B. ronchus represents a species complex. The species is therefore redescribed and its geographic range is redefined. Bairdiella veraecrucis, which is currently recognized as a junior synonym of B. ronchus, is revalidated, and a new species of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biogeographic patterns; New species; Species complex; Taxonomy; Western Atlantic.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000100205

Surprising genomic diversity in the Neotropical fish Synbranchus marmoratus (Teleostei: Synbranchidae): how many species?
Provedor de dados: Neotropical Ichthyology
Autores: Torres,Rodrigo Augusto; Roper,James Joseph; Foresti,Fausto; Oliveira,Claudio.
Chromosomes and nuclear DNA content variation in the eel Synbranchus marmoratus were studied in seven samples taken from rio Paraguay and rio Paraná basins to examine whether karyotype and nuclear DNA content variations could offer useful information for the characterization of independent evolutionary lineages in the genus and construction of evolutionary and biogeographical hypotheses. The occurrence of different karyotypes was already described for this species; however, a new cytotype from rio Miranda was discovered. Nuclear DNA content showed a wide variation among samples and individuals, ranging from 5.2 to 9.1 pg of DNA/nuclei. An analysis of variance confirmed the occurrence of significant differences among samples. In a deeper series of analyses,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish DNA; Chromosomes; Genome diversity; Karyotype; Synbranchus marmoratus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252005000200005

Chromosome analysis in Saccodon wagneri (Characiformes) and insights into the karyotype evolution of Parodontidae
Provedor de dados: Neotropical Ichthyology
Autores: Nirchio,Mauro; Masache,Maria Cecilia; Paim,Fabilene Gomes; Cioffi,Marcelo de Bello; Moreira Filho,Orlando; Barriga,Ramiro; Oliveira,Claudio; Rossi,Anna Rita.
ABSTRACT Parodontidae is a relatively small group of Neotropical characiform fishes consisting of three genera (Apareiodon, Parodon, and Saccodon) with 32 valid species. A vast cytogenetic literature is available on Apareiodon and Parodon, but to date, there is no cytogenetic data about Saccodon, a genus that contains only three species with a trans-Andean distribution. In the present study the karyotype of S. wagneri was described, based on both conventional (Giemsa staining, Ag-NOR, C-bands) and molecular (repetitive DNA mapping by fluorescent in situ hybridization) methods. A diploid chromosome number of 2n = 54 was observed in both sexes, and the presence of heteromorphic sex chromosomes of the ZZ/ZW type was detected. The W chromosome has a terminal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ag-NOR; Cytogenetics; FISH; Heterochromatin; ZW sex chromosomes.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100203

Interspecific chromosomal divergences in the genus Characidium (Teleostei: Characiformes: Crenuchidae)
Provedor de dados: Neotropical Ichthyology
Autores: Alves,José Carlos Pansonato; Paiva,Luiz Ricardo de Souza; Oliveira,Claudio; Foresti,Fausto.
Karyotypes of seven fish species of the genus Characidium, three of them studied for the first time, were characterized using conventional cytogenetic techniques (Giemsa staining, Ag-NOR, and C-banding). All species presented a diploid number of 2n=50, with only metacentric and submetacentric chromosomes, as observed in all Characidium species studied. In two species cells with one to three B chromosomes were observed. All species analyzed have a single NOR-bearing chromosome pair with morphological differences among them. Characidium cf. zebra shows heterochromatic blocks restricted to the pericentromeric regions of all chromosomes denoting the absence of a sex chromosome system. On the other hand, the species Characidium lanei, C. pterostictum, C....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotypic evolution; Sex chromosomes; B-chromosomes; Ag-NORs.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252010000100010

Phylogeography of Hypostomus strigaticeps(Siluriformes: Loricariidae) inferred by mitochondrial DNA reveals its distribution in the upper Paraná River basin
Provedor de dados: Neotropical Ichthyology
Autores: Borba,Rafael Splendore de; Zawadzki,Claudio Henrique; Oliveira,Claudio; Perdices,Anabel; Parise-Maltempi,Patrícia Pasquali; Alves,Anderson Luis.
In this study, phylogenetic and phylogeographic analyses of populations identified as Hypostomus strigaticeps from the upper Paraná River basin were conducted in order to test whether these different populations comprises cryptic species or structured populations and to assess their genetic variability. The sequences of the mitochondrial DNA ATP sintetase (subunits 6/8) of 27 specimens from 10 populations (one from Mogi-Guaçu River, five from Paranapanema River, three from Tietê River and one from Peixe River) were analyzed. The phylogeographic analysis showed the existence of eight haplotypes (A-H), and despite the ancestral haplotype includes only individuals from the Tietê River basin, the distribution of H. strigaticeps was not restricted to this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ATPase; Bayesian Inference; Haplotype; Nested clade; Phylogeny.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100111

Comparative cytogenetics in Astyanax (Characiformes: Characidae) with focus on the cytotaxonomy of the group
Provedor de dados: Neotropical Ichthyology
Autores: Tenório,Renata Cristina Claudino de Oliveira; Vitorino,Carla de Andrade; Souza,Issakar Lima; Oliveira,Claudio; Venere,Paulo Cesar.
Astyanax is a diverse group of Neotropical fishes, whose different forms occupy different environments. This great diversity is also reflected on cytogenetic aspects and molecular markers, which have repeatedly been demonstrated by cytogenetic studies. In order to characterize the karyotype of species of this genus, six species were studied: Astyanax altiparanae, A.argyrimarginatus, A. elachylepis, A. xavante, and two new species provisionally called Astyanax sp. and A. aff. bimaculatus. A detailed cytogenetic study based on conventional staining with Giemsa, AgNORs, C-banding, base-specific fluorochromes, and FISH using ribosomal genes 18S and 5S was conducted, aiming to understand some of the chromosomal mechanisms associated with the high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-banding; CMA3 and DAPI fluorochromes; Fish chromosome; RDNA genes; NORs.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300553

Cytotaxonomic diagnosis of Trichomycterus diabolus (Teleostei: Trichomycteridae) with comments about its evolutionary relationships with co-generic species
Provedor de dados: Neotropical Ichthyology
Autores: Torres,Rodrigo A.; Oliveira,Claudio; Foresti,Fausto.
The karyotype and the Ag-NOR location of a sample of Trichomycterus diabolus collected in the córrego Hortelã (Botucatu, São Paulo, Brazil) are described. The species exhibited 2n=56 chromosomes (42 metacentrics, 12 submetacentrics and 2 subtelocentrics) and the nucleolus organizing region located near to the centromere on the long arm of the largest metacentric pair. The presence of 2n=56 chromosomes in T. diabolus is an interesting characteristic since until now all cis-Andean species karyotyped presented 2n=54 chromosomes while almost all trans-Andean species presented different diploid numbers. The possible origin of this unexpected karyotypic form is discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichomycterinae; Karyotype; Chromosome evolution; Fish cytogenetic.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252004000300003

The roles of marginal lagoons in the maintenance of genetic diversity in the Brazilian migratory fishes Prochilodus argenteus and P. costatus
Provedor de dados: Neotropical Ichthyology
Autores: Melo,Bruno F.; Sato,Yoshimi; Foresti,Fausto; Oliveira,Claudio.
The rio São Francisco basin contains many endemic species, such as Prochilodus argenteus and P. costatus, which have great commercial importance. However, information about the main recruitment sites and genetic studies containing extensive sampling of these species are scarce. To investigate the roles of the marginal lagoons in the maintenance of genetic variability and in the population structure, we analyzed six microsatellite loci in nine sampling groups of P. argenteusand five sampling groups of P. costatus. Our results showed high levels of genetic variability and low values of genetic differentiation for P. argenteus (FST =0.008, P&lt; 0.05) and for P. costatus(FST =0.031, P &lt; 0.05). In addition, high values of gene flow combined with a small...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Fish migration; Microsatellite; Nursery area; Rio São Francisco basin.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300625

Karyotypic characterization of Prochilodus mariae, Semaprochilodus kneri and S. laticeps(Teleostei: Prochilodontidae) from Caicara del Orinoco, Venezuela
Provedor de dados: Neotropical Ichthyology
Autores: Oliveira,Claudio; Nirchio,Mauro; Granado,Ángel; Levy,Sara.
Fish of the family Prochilodontidae are considered one of the most important components of commercial and subsistence fishery in freshwater environments in South America. This family consists of 21 species and three genera. In the present study, the karyotypes of Prochilodus mariae, Semaprochilodus kneri, and S. laticeps from Caicara del Orinoco, Bolivar State, Venezuela were studied. The species P. mariae, S. kneri and S. laticeps exhibited 2n=54 chromosomes (40 metacentric and 14 submetacentric), a single chromosome pair with nucleolus organizer regions, and a large amount of heterochromatin found at centromeric and pericentromeric positions in almost all chromosomes. The P. mariae specimens studied displayed 0 to 3 supernumerary microchromosomes. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; C-band; NOR banding; Chromossome evolution; Characiformes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252003000100005

Karyotype characterization of Mugil incilis Hancock, 1830 (Mugiliformes: Mugilidae), including a description of an unusual co-localization of major and minor ribosomal genes in the family
Provedor de dados: Neotropical Ichthyology
Autores: Hett,Anne Kathrin; Nirchio,Mauro; Oliveira,Claudio; Siccha,Zoila Raquel; Rossi,Anna Rita; Sola,Luciana.
This study reports the description of the karyotype of Mugil incilis from Venezuela. The chromosome complement is composed of 48 acrocentric chromosomes, which uniformly decrease in size. Therefore, the homologues can not be clearly identified, with the exception of one of the largest chromosome pairs, classified as number 1, whose homologues may show a subcentromeric secondary constriction, and of chromosome pair number 24, which is considerably smaller than the others. C-banding showed heterochromatic blocks at the centromeric/pericentromeric regions of all chromosomes, which were more conspicuous on chromosomes 1, given the C-positive signals include the secondary constrictions. AgNO3 and fluorescent in situ hybridization (FISH) with 45S rDNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-banding; COI; 5S rDNA; Mullet; NORs.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100008

Karyotype description and evidence of multiple sex chromosome system X1X1X2X2/X1X2Y in Potamotrygon aff. motoro and P. falkneri (Chondrichthyes: Potamotrygonidae) in the upper Paraná River basin, Brazil
Provedor de dados: Neotropical Ichthyology
Autores: Cruz,Vanessa Paes da; Shimabukuro-Dias,Cristiane Kioko; Oliveira,Claudio; Foresti,Fausto.
Cytogenetic analysis of Potamotrygon aff. motoro and P. falkneri indicated the occurrence of an X1X1X2X2/X1X2 Y multiple sex chromosome system in both species, with 2n = 66 chromosomes for females and 2n = 65 chromosomes for males. The nucleolus organizer regions (NORs) identified using Ag-NOR technique showed that both species have multiple Ag-NORs (5 to 7 chromosomes stained). C-banding technique indicated the presence of heterochromatic blocks in the centromeric regions of almost all chromosomes in both species. Through this study there was evidence of heterogeneity in the karyotypes, which suggests that chromosomal rearrangements such as inversions and/or translocations occurred during the chromosomal evolution in two species of this genus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetic; NOR; C-band; Fish; Stingrays.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100020

Revision of the genus Pseudotocinclus (Siluriformes: Loricariidae: Hypoptopomatinae), with descriptions of two new species
Provedor de dados: Neotropical Ichthyology
Autores: Takako,Adriana Kazue; Oliveira,Claudio; Oyakawa,Osvaldo Takeshi.
The genus Pseudotocinclus is revised and two new species are described: Pseudotocinclus juquiae from the Ribeira de Iguape basin and Pseudotocinclus parahybae from the rio Paraíba do Sul basin. The presence of a small naked area on the snout tip distinguishes P. tietensis from P. parahybae and P. juquiae, which have the snout tip covered with small platelets. In addition, P. tietensis has four transverse dark-brown bands on the dorsum coalesced with the midlateral stripe instead of three, as in its congeners. Pseudotocinclus juquiae is distinguished from its congeners by having the orbital ring very prominent and conspicuous, a characteristic not found in P. tietensis or P. parahybae. These three species also differ from their congeners by meristic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Taxonomy; South America; Biogeography; Brazil; Coastal drainages.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252005000400007

Comparative description and discussion of spermiogenesis and spermatozoal ultrastructure in some species of Heptapteridae and Pseudopimelodidae (Teleostei: Siluriformes)
Provedor de dados: Neotropical Ichthyology
Autores: Quagio-Grassiotto,Irani; Spadella,Maria Angélica; Carvalho,Márcio de; Oliveira,Claudio.
The data obtained in the present study on spermiogenesis and spermatozoal ultrastructure of Pseudopimelodidae and Heptapteridae show that they share some characteristics, but greatly differ from each other. The main differences are the occurrence of type I spermiogenesis in Pseudopimelodidae and type III in Heptapteridae, the presence of nuclear fossa in Pseudopimelodidae and its absence in Heptapteridae, the presence of long midpiece in Pseudopimelodidae and short midpiece in Heptapteridae, the presence of cytoplasmic canal in Pseudopimelodidae and its absence in Heptapteridae, the presence of many large vesicles in the midpiece of Pseudopimelodidae and the presence of very long vesicles placed in the peripheral distal region in Heptapteridae, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catfish; Spermatid; Sperm; Structure; Phylogeny.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252005000300008

Revalidation and redescription of Steindachnerina nigrotaenia and redescription of S. insculpta (Characiformes: Curimatidae)
Provedor de dados: Neotropical Ichthyology
Autores: Britski,Heraldo A.; Melo,Bruno F.; Vari,Richard P.; Oliveira,Claudio.
ABSTRACT Steindachnerina nigrotaenia is resurrected from the synonym of S. brevipinna and considered a valid species. The previous designation of the lectotype of S. nigrotaenia is considered invalid and a new lectotype is designated herein. Steindachnerina nigrotaenia and S. insculpta are redescribed based on type specimens and on additional material from the rio Paraguai and the upper rio Paraná basins, respectively. The two species can be separated by the number of scales of the lateral line and of the transverse series and by phylogenetic analyses of molecular data.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ostariophysi; Neotropical fishes; Systematics; Taxonomy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000100207

New species of Moenkhausia Eigenmann, 1903 (Characiformes: Characidae) with comments on the Moenkhausia oligolepis species complex
Provedor de dados: Neotropical Ichthyology
Autores: Benine,Ricardo C.; Mariguela,Tatiane C.; Oliveira,Claudio.
A new species of Moenkhausia is described from tributaries of the rio Paraguay, Brazil. The new species is diagnosed from congeners by characters related to body coloration, the number of lateral line scales, the degree of poring of the lateral line, and number of scales rows above and below the lateral line. Molecular analyses using partial sequences of the mitochondrial gene Cytochrome Oxidase I from specimens of the new species and specimens belonging to morphologically similar species demonstrated that the new species is easily differentiated by their high genetic distance and by their position in the phylogenetic hypothesis obtained through the Maximum Parsimony methodology. The analyses of three samples of M. oligolepis also revealed that they have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Taxonomy; Systematic; Molecular Phylogeny; COI gene; Moenkhausia sanctaefilomenae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000200005

Description of a new species of Moenkhausia (Characiformes: Characidae) from the upper Paraguay basin, Central Brazil, with comments on its phylogenetic relationships
Provedor de dados: Neotropical Ichthyology
Autores: Britzke,Ricardo; Troy,Waldo P.; Oliveira,Claudio; Benine,Ricardo C.
ABSTRACT A new species of Moenkhausia is described from tributaries of the upper rio Sepotuba, Paraguay basin, Brazil. The new species is distinguished from its congeners by a combination of characters, including an inconspicuous oval-shaped vertically elongated humeral blotch, extending horizontally from third through five lateral-line scales, and vertically from third row above lateral line to first row below it, followed by a diffuse field of dark chromatophores in the flank, combined with a well-defined dark line at the base of the anal fin. Furthermore, the phylogenetic position of the new species is presented based on molecular data, showing a close relationship among species of Moenkhausia and Hemigrammus that have a well-defined dark line at the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemigrammus; Molecular phylogeny; Neotropical tetras; Taxonomy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000200211

Spermiogenesis and spermatozoa ultrastructure in the Serrasalminae (Ostariophysi: Characiformes) with further evidence on the relationships of the piranhas and pacus
Provedor de dados: Neotropical Ichthyology
Autores: Gusmão-Pompiani,Priscila; Malabarba,Luiz R.; Oliveira,Claudio; Quagio-Grassiotto,Irani.
Spermatozoa and spermiogenesis ultrastructure were studied in the serrasalmine species Piaractus mesopotamicus, Mylossoma duriventre, Serrasalmus maculatus, and Metynnis mola and two distinct patterns may be recognized: the first common to Mylossoma, Serrasalmus and Metynnis, and the other, characteristic of Piaractus. The latter pattern is more similar to the conditions found in Salminus and Brycon. On the other hand, serrasalmine spermatozoa also share characteristics with the spermatozoa of species of the superfamily Anostomoidea. The phylogenetic significance of these characters is discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characid; Spermatid; Spermatozoa; Phylogeny; Morphology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000300005

Molecular identification of Atlantic goliath grouper Epinephelus itajara (Lichtenstein, 1822) (Perciformes: Epinephelidae) and related commercial species applying multiplex PCR
Provedor de dados: Neotropical Ichthyology
Autores: Damasceno,Júnio S.; Siccha-Ramirez,Raquel; Oliveira,Claudio; Mendonça,Fernando F.; Lima,Arthur C.; Machado,Leonardo F.; Tosta,Vander C.; Farro,Ana Paula C.; Hostim-Silva,Maurício.
ABSTRACT The Atlantic goliath grouper, Epinephelus itajara , is a critically endangered species, threatened by illegal fishing and the destruction of its habitats. A number of other closely related grouper species found in the western Atlantic are also fished intensively. While some countries apply rigorous legislation, illegal harvesting followed by the falsification of fish products, which impedes the correct identification of the species, is a common practice, allowing the catch to be marketed as a different grouper species. In this case, molecular techniques represent an important tool for the monitoring and regulation of fishery practices, and are essential for the forensic identification of a number of different species. In the present study,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; COI; Endangered species; Fishery management; Forensic genetics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000300203

Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae)
Provedor de dados: Neotropical Ichthyology
Autores: Oliveira,Maria Lígia M. de; Utsunomia,Ricardo; Pansonato-Alves,José Carlos; Scacchetti,Priscilla C.; Primo,Cleberson C.; Vicari,Marcelo R.; Artoni,Roberto F.; Centofante,Liano; Moreira-Filho,Orlando; Oliveira,Claudio; Foresti,Fausto.
Trichomycterus is a specious fish genus within Trichomycterinae and displays remarkable karyotype diversity. However, knowledge about their genomic structure and location of repetitive sequence is still limited. In order to better understand the karyotype diversification, we analyzed nine species of Trichomycterus using classical and molecular cytogenetic techniques. Results revealed a conserved diploid chromosome number of 2n=54 chromosomes in all analyzed species, although remarkable differences on the constitutive heterochromatin distribution were observed. In addition, while the 18S rDNA showed a conserved distribution pattern, the 5S rDNA sites showed a quite diverse location considering the analyzed species. Remarkably, both ribosomal genes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype diversity; Repetitive DNA; RDNA; Supernumerary chromosomes.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200211

Distributions and phylogeographic data of rheophilic freshwater fishes provide evidences on the geographic extension of a central-brazilian amazonian palaeoplateau in the area of the present day Pantanal Wetland
Provedor de dados: Neotropical Ichthyology
Autores: Ribeiro,Alexandre C.; Jacob,Rodrigo M.; Silva,Ronnayana R. S. R.; Lima,Flávio C. T.; Ferreira,Daniela C.; Ferreira,Katiane M.; Mariguela,Tatiane C.; Pereira,Luiz H. G.; Oliveira,Claudio.
The analysis of the distribution patterns presented by examples of freshwater fishes restricted to headwater habitat: the anostomid Leporinus octomaculatus, the characins Jubiaba acanthogaster, Oligosarcus perdido, Moenkhausia cosmops, Knodus chapadae, Planaltina sp., the loricariid Hypostomus cochliodon, and the auchenipterid Centromochlus sp. provided evidences of a relatively recent shared history between the highlands of the upper rio Paraguay and adjoining upland drainage basins. Restricted to headwater of the uplands in the upper rio Paraguay and adjoining basins, these species provide biological evidence of the former extension of the central Brazilian plateau before the origin of the Pantanal Wetland. Disjunction took place due to an ecological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Brazilian highlands; Historical biogeography; Paleodrainage; Tectonic evolution.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000200319

Ichthyofauna of streams of the Rio Sapucaí basin, upper Rio Paraná system, Minas Gerais, Brazil
Provedor de dados: Biota Neotropica
Autores: Azevedo-Santos,Valter M.; Britski,Heraldo A.; Oliveira,Claudio; Benine,Ricardo C.
Abstract: The Rio Sapucaí basin, in Minas Gerais State, Brazil, is one of the many watersheds of the upper Rio Paraná system. Ichthyofauna surveys in this basin, in general, are scarce. In addition, small rivers and streams of the region have been targets of anthropogenic actions (e.g., pollution) - which suggest that more ichthyological studies must be performed within the watershed. In this study we provide a survey of species that occur within three streams of the lower Rio Sapucaí basin. Samples were collected in April, July, and November 2017 and in May 2018. Collections resulted in 349 individuals belonging to 28 species, five orders, and 12 families. Among our findings are three putatively undescribed species and the first record of Oligosarcus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fishes; Inventory; Oligosarcus argenteus; Pareiorhina hyptiorhachis; Undescribed species.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032019000100309

Estimated frequency of B-chromosomes and population density of Astyanax scabripinnis paranae in a small stream
Provedor de dados: Brazilian Journal of Genetics
Autores: Porto-Foresti,Fábio; Oliveira,Claudio; Maistro,Edson Luis; Foresti,Fausto.
A study was made of the frequency of B-chromosomes and the population density of Astyanax scabripinnis paranae (Pisces, Characidae, Tetragonopterinae) from three stretches of the Cascatinha stream (Botucatu, SP). In the first stretch the population was estimated to be about 212 individuals and among 35 karyotyped specimens, 23 carried one macro B-chromosome; in the second stretch the population was estimated to be about 650 individuals and among 20 specimens karyotyped, two possessed one macro B-chromosome; in the third stretch the population was estimated to be about 107 individuals and among 10 specimens karyotyped, one carried one macro B-chromosome. A significant difference was observed in the frequency of macro B-chromosomes in females (57%) and males...
Tipo: Info:eu-repo/semantics/article
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000300004