Consulta: subjectFacets:"Cytogenetics"
Registros recuperados: 87
Data/hora: 10/06/2024 18:20:54

Cytogenetic characterization of F1, F2 and backcross hybrids of the Neotropical catfish species Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum (Pimelodidae, Siluriformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Prado,Fernanda Dotti do; Nunes,Tatiana Leite; Senhorini,José Augusto; Bortolozzi,Jehud; Foresti,Fausto; Porto-Foresti,Fábio.
The cytogenetic characteristics of Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum and their F1, F2 and backcross hybrids were assessed by using chromosome banding techniques. The diploid number of 56 chromosomes was constant in all species and lineages, with a karyotypic formula containing 20 metacentric, 12 submetacentric, 12 subtelocentric and 12 acrocentric chromosomes. Nucleolar organizer regions (NORs) were identified in two subtelocentric chromosomes in the parents and hybrids, with partial nucleolar dominance in F1 and F2 specimens. Heterochromatic blocks were detected in the terminal and centromeric regions of some chromosomes in all individuals. For parental and hybrid lineages, 18S ribosomal clusters corresponding to NORs and 5S...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Backcrossing; Cytogenetics; Interspecific hybrids; Post-F1 hybrids; Pseudoplatystoma.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100008

Karyotypic description of four species of Trigona (Jurine, 1807) (Hymenoptera, Apidae, Meliponini) from the State of Mato Grosso, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Kleber França; Brito,Rute Magalhães; Miyazawa,Carlos Suetoshi.
The genus Trigona contains at least 31 species, but there have been few cytogenetic studies of this group. In this work, four species of Trigona (T. branneri, T. chanchamayoensis, T. hyalinata, and T. recursa) from the municipality of Cuiabá, in the State of Mato Grosso, Brazil, were studied. In all of the species, the females had 2n = 34 chromosomes and the males had n = 17. The C-banding patterns showed that the karyotypes of these species consisted mainly of acrocentric and pseudoacrocentric chromosomes. These cytogenetic findings should useful in future phylogenetic studies of this group.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Karyotype; Meliponini; Stingless bees; Trigona.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200010

Cytogenetics of the Brazilian Bolitoglossa paraensis (Unterstein, 1930) salamanders (Caudata, Plethodontidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Jéssica Barata da; Suárez,Pablo; Nagamachi,Cleusa Yoshiko; Carter,Timothy Frederick; Pieczarka,Julio Cesar.
Plethodontid salamanders of genus Bolitoglossa constitute the largest and most diverse group of salamanders, including around 20% of living caudate species. Recent studies have indicated the occurrence of five recognized species in the Brazilian Amazon Rainforest. We present here the first cytogenetic data of a Brazilian salamander, which may prove to be a useful by contribution to the cytotaxonomy of the genus. Specimens were collected near the "type" locality (Utinga, Belém, PA, Brazil). Chromosomal preparations from duodenal epithelial cells and testes were subjected to Giemsa staining, C-banding and DAPI/CMA3 fluorochrome staining. All specimens showed a karyotype with 13 bi-armed chromosome pairs (2n = 26). Nucleolar Organizer Regions, evidenced by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salamanders; Bolitoglossa; Karyotypes; Brazilian Amazon; Cytogenetics.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400008

American marsupials chromosomes: why study them?
Provedor de dados: Genetics and Molecular Biology
Autores: Svartman,Marta.
Marsupials, one of the three main groups of mammals, are only found in Australia and in the American continent. Studies performed in Australian marsupials have demonstrated the great potential provided by the group for the understanding of basic genetic mechanisms and chromosome evolution in mammals. Genetic studies in American marsupials are relatively scarce and cytogenetic data of most species are restricted to karyotype descriptions, usually without banding patterns. Nevertheless, the first marsupial genome sequenced was that of Monodelphis domestica, a South American species. The knowledge about mammalian genome evolution and function that resulted from studies on M. domestica is in sharp contrast with the lack of genetic data on most American...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marsupials; Cytogenetics; Chromosome evolution.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400002

Nine karyomorphs for spiny rats of the genus Proechimys (Echimyidae, Rodentia) from North and Central Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Machado,Taís; Silva,Maria José de J.; Leal-Mesquita,Emygdia Rosa; Carmignotto,Ana Paula; Yonenaga-Yassuda,Yatiyo.
Spiny rats of the genus Proechimys are morphologically diverse, widely distributed and have diploid numbers ranging from 2n = 14-16 to 2n = 62. In this paper we present cytogenetical data and brief comments on morphological and biogeographical issues related to spiny rats. In our sample of 42 spiny rats collected from 12 Brazilian Amazonian tropical rainforest and the Cerrado (Brazilian savanna) sites we detected nine karyological entities: four different karyomorphs with 2n = 30, three with 2n = 28, one with 2n = 15 and one with 2n = 44. Based on qualitative morphological characters these karyomorphs can be allocated to five species within the goeldii, guyannensis and longicaudatus species groups.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proechimys; Rodents; Cytogenetics; Karyomorph; Morphology.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500007

Comparative cytogenetic analysis in the species Uroderma magnirostrum and U. bilobatum (cytotype 2n = 42) (Phyllostomidae, Stenodermatinae) in the Brazilian Amazon
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Adailton Moreira da; Marques-Aguiar,Suely Aparecida; Barros,Regina Maria de Souza; Nagamachi,Cleusa Yoshiko; Pieczarka,Julio Cesar.
The genus Uroderma includes two species: U. magnirostrum and U. bilobatum. These species are characterized by their high degree of karyotypic evolution, diverging from most other species of the subfamily Stenodermatinae, which have a lower degree of chromosomic evolution. The present study reports the first banding patterns of U. magnirostrum (G-, C-banding and Ag-NOR) and U. bilobatum (C-banding and Ag-NOR). The chromosomic data in conventional staining of U. magnirostrum (2n = 36, NF = 62) and U. bilobatum (cytotype 2n = 42, NF = 50) are equivalent to that described in the literature. When compared, chromosomal homeologies are found in both karyotypes, as well as differences, confirming that karyotypic evolution in the Uroderma genus is intense. Fission,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Chromosomal evolution; Phyllostomidae; Uroderma.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200012

Cytogenetic analysis of three sea catfish species (Teleostei, Siluriformes, Ariidae) with the first report of Ag-NOR in this fish family
Provedor de dados: Genetics and Molecular Biology
Autores: Nirchio,Mauro; Martinez,Emanuel Ricardo Monteiro; Foresti,Fausto; Oliveira,Claudio.
Despite their ecological and economical importance, fishes of the family Ariidae are still genetically and cytogenetically poorly studied. Among the 133 known species of ariids, only eight have been karyotyped. Cytogenetic analyses performed on Genidens barbus and Sciades herzbergii revealed that both species have 2n = 56 chromosomes and Cathorops aff. mapale has 2n = 52 chromosomes: Genidens barbus has 10 Metacentrics (M), 14 Submetacentrics (SM), 26 Subtelocentrics (ST), and 6 Acrocentrics (A), Sciades herzbergii has 14M, 20SM, 18ST and 4A, whereas Cathorops aff. mapale has 14M, 20SM, and 18ST. The nucleolus organizer regions (NORs) were found in a single chromosome pair on the short arm of a large-sized ST pair in Genidens barbus and on the short arm of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Ag-NORs; Chromosome evolution; Chromosome rearrangements; Fish.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200011

Karyotype characterization and nucleolar organizer regions of marsupial species (Didelphidae) from areas of Cerrado and Atlantic Forest in Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Pereira,Núbia P.; Ventura,Karen; Silva Júnior,Magno C.; Silva,Daniela de Melo e; Yonenaga-Yassuda,Yatiyo; Pellegrino,Katia C. M.
The karyotypes of 23 specimens belonging to 16 species from nine genera of Brazilian marsupials (family Didelphidae) were studied. The animals were collected in eight localities of Cerrado or Atlantic Forest biomes in the states of Goiás, Tocantins and São Paulo. The karyotypes were analyzed after conventional Giemsa staining and silver staining of the nucleolus organizer regions (Ag-NORs). New karyotypic data were obtained for  Gracilinanus microtarsus  (2n = 14, FN = 24), Marmosops paulensis  (2n = 14, FN = 24) , Micoreus paraguayanus  (2n = 14, FN = 20) and  Monodelphis rubida  (2n = 18, FN = 32) and are discussed in detail. The karyotypes of  G. microtarsus ,  M. paulensis  and  M. paraguayanus  include three large pairs of submetacentrics (pairs 1, 2...
Palavras-chave: Marsupials; Karyotypes; Cytogenetics; Didelphidae; NORs.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500014

The karyotype of three Brazilian Terrarana frogs (Amphibia, Anura) with evidence of a new Barycholos species
Provedor de dados: Genetics and Molecular Biology
Autores: Siqueira,Sérgio; Aguiar Jr.,Odair; Pansonato,André; Giaretta,Ariovaldo A.; Strüssmann,Christine; Martins,Itamar; Recco-Pimentel,Shirlei M.
A recent substantial rearrangement of the 882 described eleutherodactyline frog species has considerably improved the understanding of their systematics. Nevertheless, many taxonomic aspects of the South American eleutherodactyline species remain unknown and require further investigation using morphological, cytogenetic and molecular approaches. In this work, the karyotypes of the Brazilian species Ischnocnema juipoca (Atibaia and Campos do Jordão, SP), Barycholos cf. ternetzi (Uberlândia, MG, and Porto Nacional, TO), and Pristimantis crepitans (Chapada dos Guimarães and São Vicente, MT) were analyzed using Giemsa staining, Ag-NOR labeling, and C-banding techniques. All individuals had a diploid number of 22 chromosomes, but the Fundamental Numbers were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Barycholos; Ischnocnema; Pristimantis; Ag-NOR; C-banding.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300008

Genetics, evolution and conservation of Bromeliaceae
Provedor de dados: Genetics and Molecular Biology
Autores: Zanella,Camila M.; Janke,Aline; Palma-Silva,Clarisse; Kaltchuk-Santos,Eliane; Pinheiro,Felipe G.; Paggi,Gecele M.; Soares,Luis E.S.; Goetze,Márcia; Büttow,Miriam V.; Bered,Fernanda.
Bromeliaceae is a morphologically distinctive and ecologically diverse family originating in the New World. Three centers of diversity, 58 genera, and about 3,140 bromeliad species are currently recognized. We compiled all of the studies related to the reproductive biology, genetic diversity, and population structure of the Bromeliaceae, and discuss the evolution and conservation of this family. Bromeliads are preferentially pollinated by vertebrates and show marked variation in breeding systems, from predominant inbreeding to predominant outcrossing, as well as constancy in chromosome number (2n = 2x = 50). Autogamous or mixed mating system bromeliads have a high inbreeding coefficient (F IS), while outcrossing species show low F IS. The degree of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromeliads; Cytogenetics; Genetic diversity; Population structure; Reproductive biology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600017

Polymorphism of Sooty-fronted Spinetail (Synallaxis frontalis Aves: Furnariidae): Evidence of chromosomal rearrangements by pericentric inversion in autosomal macrochromosomes
Provedor de dados: Genetics and Molecular Biology
Autores: Souza,Marcelo Santos de; Barcellos,Suziane Alves; Costa,Alice Lemos; Kretschmer,Rafael; Garnero,Analía Del Valle; Gunski,Ricardo José.
Abstract The Passeriformes is the most diverse and cytogenetically well-known clade of birds, comprising approximately 5,000 species. The sooty-fronted spinetail (Synallaxis frontalis Aves: Furnariidae) species, which belongs to the order Passeriformes, is typically found in South America, where it is widely distributed. Polymorphisms provide genetic variability, important for several evolutionary processes, including speciation and adaptation to the environment. The aim of this work was to analyze the possible cytotypes and systemic events involved in the species polymorphism. Of the sampled 19 individuals, two thirds were polymorphic, an event supposedly linked to mutations resulting from genomic evolution that can be transmitted hereditarily. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal polymorphism; Evolution; Cytogenetics.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100062

Somatic and germ cell cytogenetic studies and AZF microdeletion screening in infertile men
Provedor de dados: Genetics and Molecular Biology
Autores: Carrara,Rita C. V.; Yamasaki,Rui; Mazucatto,Luís F.; Veludo,Maria A. Llorach; Sartorato,Edi L.; Pina-Neto,João M.
Clinical and cytogenetic studies were performed in 65 infertile individuals, and 56 of them were also screened for microdeletions in Yq11 (AZF region). Relevant environmental etiological factors were identified in 10 cases (15.4%). Sertoli-cell-only syndrome was diagnosed in six patients (9,2%). Karyotype abnormalities were detected in six individuals, and five other patients presented desynapsis of bivalents in meiosis. Three out of the 56 patients studied were carriers of microdeletions in the AZF region, one of them also presenting a chromosomal mosaicism for an extra i(22p).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Male infertility; Cytogenetics; Microdeletions; AZF; Meiotic anomalies.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400002

Comparative cytogenetic studies of Bufo ictericus, B. paracnemis (Amphibia, Anura) and an intermediate form in sympatry
Provedor de dados: Genetics and Molecular Biology
Autores: Azevedo,MFC; Foresti,F.; Ramos,PRR; Jim,J.
Specimens of Bufo ictericus, Bufo paracnemis and a third type, considered an intermediate subgroup between these species, were cytogenetically studied by conventional Giemsa staining, C-banding and staining of the nucleolus organizer region (NOR). The nuclear DNA content and seroproteins were also analyzed to characterize these species, and verify the possibility of hybridization between them. Karyotypes and cytogenetic markers were essentially equal on the basis of the methods used. The DNA nuclear content found was 6.25 ± 0.30 pg/DNA in Bufo ictericus; 7.57 ± 0.40 pg/DNA in Bufo paracnemis and 7.04 ± 0.29 pg/DNA in the intermediate subgroup. Eletrophoresis of total blood serum in Bufo ictericus, Bufo paracnemis and the intermediate specimens revealed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bufo; Hybridization; Cytogenetics; Nuclear DNA; Seroproteins.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300012

Karyotype characterization of Trigona fulviventris Guérin, 1835 (Hymenoptera, Meliponini) by C banding and fluorochrome staining: report of a new chromosome number in the genus
Provedor de dados: Genetics and Molecular Biology
Autores: Domingues,Alayne Magalhães Trindade; Waldschmidt,Ana Maria; Andrade,Sintia Emmanuelle; Andrade-Souza,Vanderly; Alves,Rogério Marco de Oliveira; Silva Junior,Juvenal Cordeiro da; Costa,Marco Antônio.
Although many species of the genus Trigona have been taxonomically described, cytogenetic studies of these species are still rare. The aim of the present study was to obtain cytogenetic data by conventional staining, C banding and fluorochrome staining for the karyotype characterization of the species Trigona fulviventris. Cytogenetic analysis revealed that this species possesses a diploid chromosome number of 2n = 32, different from most other species of this genus studied so far. This variation was probably due to the centric fusion in a higher numbered ancestral karyotype, this fusion producing the large metacentric chromosome pair and the lower chromosome number observed in Trigona fulviventris. Heterochromatin was detected in the pericentromeric...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytogenetics; Heterochromatin; Evolution; Stingless bees.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300009

Meiotic chromosomes and nucleolar behavior in testicular cells of the grassland spittlebugs Deois flavopicta, Mahanarva fimbriolata and Notozulia entreriana (Hemiptera, Auchenorrhyncha)
Provedor de dados: Genetics and Molecular Biology
Autores: Castanhole,Márcia Maria Urbanin; Pereira,Luis Lênin Vicente; Souza,Hederson Vinicius de; Valério,José Raul; Barbosa,Leonardo Rodrigues; Itoyama,Mary Massumi.
Spittlebugs annually infest pastures and cause severe damage, representing a serious problem for the tropical American beef cattle industry. Spittlebugs are an important biotic constraint to forage production and there is a lack of cytogenetic data for this group of insects. For these reasons, we conducted this work, in which the spermatogenesis and nucleolar behavior of Deois flavopicta, Mahanarva fimbriolata and Notozulia entreriana were studied. The males possessed testes in the shape of a "bunch of grapes"; a variable number of testicular lobes per individual and polyploid nuclei composed of several heteropycnotic bodies. A heteropycnotic area was located in the periphery of the nucleus (prophase I); the chiasmata were terminal or interstitial;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal characterization; Karyotype; Cytogenetics; Spermatogenesis; Holocentric chromosomes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200008

Occurrence of B chromosomes in Tetragonisca Latreille, 1811 (Hymenoptera, Apidae, Meliponini): a new contribution to the cytotaxonomy of the genus
Provedor de dados: Genetics and Molecular Biology
Autores: Barth,Adriane; Fernandes,Anderson; Pompolo,Silvia das Graças; Costa,Marco Antônio.
Tetragonisca angustula and Tetragonisca fiebrigi have recently been listed as valid species. This study aimed to cytogenetically investigate both species, emphasizing the new registry of B chromosomes in the tribe Meliponini. We analyzed colonies of T. angustula and T. fiebrigi collected at Tangará da Serra, Mato Grosso, Brazil, through conventional Giemsa staining, C-banding, and base-specific fluorochrome staining (CMA3/DAPI). T. angustula showed 2n = 34 chromosomes in females and n = 17 in males, with karyotype formula 2K = 34A M. T. fiebrigi showed numeric variation, with chromosome number varying from 2n = 34 to 2n = 36 in females and from n = 17 to n=18in males, with karyotype formula 2K = 32A M+2A Mc and 2K = 32A M+2A Mc + 1 or 2 B-chromosomes. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stingless bees; Cytogenetics; Interspecific differentiation; B chromosomes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100014

Comparative karyotype analysis in diploid and triploid Dolichoplana carvalhoi (Tricladida, Terricola, Rhynchodemidae) from Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Alvarez,Luciana; Almeida,Eunice Judith Cardoso de.
In this work, we present cytogenetic data for the land planarian Dolichoplana carvalhoi. Two different karyotypes, one diploid (2n = 2x = 14 chromosomes) and one triploid (2n = 3x = 21 chromosomes), corresponding to two morphological body patterns, are described. Chromosomes from regenerating blastema were studied after routine Giemsa staining and CBG banding. Our analyses revealed heteromorphisms in chromosomes 2, 3 and 4 of the diploid karyotype and in chromosomes 1, 3 and 7 of the triploid karyotype. Further studies are needed in order to determine if the two morphological patterns of D. carvalhoi represent distinct species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Land planarians; Dolichoplana carvalhoi; Cytogenetics; Triploidy; Heteromorphisms; C-bands.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300013

Chromosome polymorphism in the Brazilian dwarf brocket deer, Mazama nana (Mammalia, Cervidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Abril,Vanessa Veltrini; Duarte,José Maurício Barbanti.
The Brazilian dwarf brocket deer (Mazama nana) is the smallest deer species in Brazil and is considered threatened due to the reduction and alteration of its habitat, the Atlantic Rainforest. Moreover, previous work suggested the presence of intraspecific chromosome polymorphisms which may contribute to further population instability because of the reduced fertility arising from the deleterious effects of chromosome rearrangements during meiosis. We used G- and C-banding, and nucleolus organizer regions localization by silver-nitrate staining (Ag-NOR) to investigate the causes of this variation. Mazama nana exhibited eight different karyotypes (2n = 36 through 39 and FN = 58) resulting from centric fusions and from inter and intraindividual variation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mazama nana; Brazilian dwarf brocket deer; Cytogenetics; Chromosome polymorphism; Centric fusions.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100011

Sex chromosome system ZZ/ZW in Apareiodon hasemani Eigenmann, 1916 (Characiformes, Parodontidae) and a derived chromosomal region
Provedor de dados: Genetics and Molecular Biology
Autores: Bellafronte,Elisangela; Schemberger,Michelle Orane; Artoni,Roberto Ferreira; Moreira Filho,Orlando; Vicari,Marcelo Ricardo.
Parodontidae fish show few morphological characteristics for the identification of their representatives and chromosomal analyses have provided reliable features for determining the interrelationships in this family. In this study, the chromosomes of Apareiodon hasemani from the São Francisco River basin, Brazil, were analyzed and showed a karyotype with 2n = 54 meta/submetacentric chromosomes, and a ZZ/ZW sex chromosome system. The study revealed active NORs located on pair 11 and additional 18S rDNA sites on pairs 7 and 22. The 5S rDNA locus was found in pair 14. It showed a pericentric inversion regarding the ancestral condition. The satellite DNA pPh2004 was absent in the chromosomes of A. hasemani, a shared condition with most members of Apareiodon....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; FISH; 18S rDNA; 5S rDNA; Neotropical fishes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500010

Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera)
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Laiana Cristina da; Azeredo-Oliveira,Maria Tercília Vilela de; Tartarotti,Ester.
Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triatoma klugi; Triatomine; Cytogenetics; Nucleolus; Karyotype; Meiosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300008

Nucleolar organizer regions in Sittasomus griseicapillus and Lepidocolaptes angustirostris (Aves, Dendrocolaptidae): evidence of a chromosome inversion
Provedor de dados: Genetics and Molecular Biology
Autores: Barbosa,Marcelo de Oliveira; Silva,Rubens Rodrigues da; Correia,Vanessa Carolina de Sena; Santos,Luana Pereira dos; Garnero,Analía del Valle; Gunski,Ricardo José.
Cytogenetic studies in birds are still scarce compared to other vertebrates. Woodcreepers (Dendrocolaptidae) are part of a highly specialized group within the Suboscines of the New World. They are forest birds exclusive to the Neotropical region and similar to woodpeckers, at a comparable evolutionary stage. This paper describes for the first time the karyotypes of the Olivaceous and the Narrow-billed Woodcreeper using conventional staining with Giemsa and silver nitrate staining of the nucleolar organizer regions (Ag-NORs). Metaphases were obtained by fibular bone marrow culture. The chromosome number of the Olivaceous Woodcreeper was 2n = 82 and of the Narrow-billed Woodcreeper, 2n = 82. Ag-NORs in the largest macrochromosome pair and evidence of a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Woodcreepers; Ag-NOR; Chromosomal inversion.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100010

Use of AFLP and RAPD molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the Patagonian Bromus setifolius complex
Provedor de dados: Genetics and Molecular Biology
Autores: García,Ana M.; Schrauf,Gustavo E.; González,Graciela; Poggio,Lidia; Naranjo,Carlos A.; Dupal,Marck P.; Spangenberg,Germán C.; Forster,John W.
Bromus setifolius var. pictus (Hook) Skottsb., B. setifolius var. setifolius Presl. and B. setifolius var. brevifolius Ness are three native Patagonian taxa in the section Pnigma Dumort of the genus Bromus L. AFLP and RAPD analysis, in conjunction with genetic distance measurements and statistical techniques, revealed variation within this group and indicated that B. setifolius var. brevifolius was closely related to B. setifolius var. pictus, with both taxa being more distantly related to B. setifolius var. setifolius. Cytogenetic analysis confirmed the chromosomal number of B. setifolius var. pictus (2n = 70) and B. setifolius var. setifolius (2n = 28) and showed for the first time that B. setifolius var. brevifolius had 2n = 70. The combination of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Argentina; Bromus setifolius complex; Cytogenetics; Patagonia; RAPD.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200018

The distribution of 45S rDNA sites in bird chromosomes suggests multiple evolutionary histories
Provedor de dados: Genetics and Molecular Biology
Autores: Degrandi,Tiago Marafiga; Gunski,Ricardo José; Garnero,Analía del Valle; Oliveira,Edivaldo Herculano Correa de; Kretschmer,Rafael; Souza,Marcelo Santos de; Barcellos,Suziane Alves; Hass,Iris.
Abstract The distribution of 45S rDNA cluster in avian karyotypes varies in different aspects, such as position, number of bearer chromosomes, and bearers being macro- or microchromosomes. The present study investigated the patterns of variation in the 45S rDNA-bearer chromosomes of birds in order to understand the evolutionary dynamics of the cluster configuration and its contribution to the evolution of bird karyotypes. A total of 73 bird species were analyzed, including both published data and species for which rDNA-FISH was conducted for the first time. In most birds, the 45S rDNA clusters were located in a single pair of microchromosomes. Hence, the location of 45S rDNA in macrochromosomes, observed only in Neognathae species, seems to be a derived...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FISH; Chromosome; Chromosome evolution; Cytogenetics; Aves.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400601

Physical mapping of the 18S and 5S ribosomal genes in nine Characidae species (Teleostei, Characiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Peres,Wellington Adriano Moreira; Bertollo,Luiz Antonio Carlos; Moreira Filho,Orlando.
Characidae is one of the largest fish families of the Neotropical region, and presenting a pronounced morphological variability, certainly does not constitute a monophyletic group. The cytogenetical data also show a large chromosomal variation and can provide important information for a better understanding of the relationships between the species of this group. 18S and 5S rDNA probes were used in the present study for the chromosomal mapping in different Characidae species from the São Francisco River (Astyanax lacustris, Astyanax scabripinnis, Hasemania nana, Piabina argentea, Orthospinus franciscensis, Serrapinnus heterodon, Serrapinnus piaba and Myleus micans) and Alto Paraná (Astyanax altiparanae) basins. Species with a single pair of chromosomes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Neotropical fish; Fluorescent in situ hybridization (FISH); RDNA probe.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200009

A remarkable autosomal heteromorphism in Pseudoryzomys simplex 2n = 56; FN = 54-55 (Rodentia, Sigmodontinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Moreira,Camila Nascimento; Di-Nizo,Camilla Bruno; Silva,Maria José de Jesus; Yonenaga-Yassuda,Yatiyo; Ventura,Karen.
Pseudoryzomys simplex, the false rice rat, is a monotypic genus of the Oryzomyini tribe (Sigmodontinae) distributed in part of Bolivia, Paraguay, Argentina and Brazil. Its diploid number has been described as 56 acrocentric chromosomes decreasing in size and no karyotype figure has been depicted. Herein, we present karyotypic data on P. simplex, including chromosome banding and molecular fluorescent in situ hybridization using telomeric sequences and the whole X-chromosome of its sister clade Holochilus brasiliensis (HBR) as probes. A case of remarkable autosomal heteromorphism due to the presence of a whole heterochromatic arm leading to the variability of FN is reported, as well as the occurrence of regions of homology between the X and Y chromosomes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Fluorescent in situ hybridization; Heterochromatin; Oryzomyini.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200010

Chromosome mapping of ribosomal genes and histone H4 in the genus Radacridium (Romaleidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Anjos,Allison; Loreto,Vilma; Souza,Maria José de.
In this study, two species of Romaleidae grasshoppers, Radacridium mariajoseae and R.nordestinum, were analyzed after CMA3/DA/DAPI sequential staining and fluorescence in situ hybridization (FISH) to determine the location of the 18S and 5S rDNA and histone H4 genes. Both species presented karyotypes composed of 2n = 23, X0 with exclusively acrocentric chromosomes. CMA3+ blocks were detected after CMA3/DA/DAPI staining in only one medium size autosome bivalent and in the X chromosome in R. mariajoseae. On the other hand, all chromosomes, except the L1 bivalent, of R. nordestinum presented CMA3+ blocks. FISH analysis showed that the 18S genes are restricted to the X chromosome in R. mariajoseae, whereas these genes were located in the L2,S9 and S10...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; FISH; Fluorochromes; Grasshoppers; RDNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300006

Cytotaxonomy of Parodon nasus and Parodon tortuosus (Pisces, Characiformes): A case of synonymy confirmed by cytogenetic analyses
Provedor de dados: Genetics and Molecular Biology
Autores: Bellafronte,Elisangela; Margarido,Vladimir Pavan; Moreira-Filho,Orlando.
Morphological and cytogenetical studies were carried out on the freshwater fish Parodon nasus and Parodon tortuosus in order to evaluate a putative synonymy. The diploid chromosome number observed in both species was 2n = 54 (48M/SM and 6ST) with no differences between the sexes. Despite slight differences in the pattern of heterochromatin distribution and the number of cusps in symphysean teeth, the location of nucleolar organizer regions (NORs) and 5S rRNA genes (both species-specific features) were similar in both species. The remarkable similarity observed between these allopatric species supports recent taxonomical reviews indicating that P. tortuosus is a synonym for P. nasus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Parodontidae; Systematics; 18S rDNA; 5S rDNA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500010

Low cytomolecular diversification in the genus Stylosanthes Sw. (Papilionoideae, Leguminosae)
Provedor de dados: Genetics and Molecular Biology
Autores: Franco,Ana Luiza; Figueredo,Amanda; Pereira,Lívia de Moraes; Sousa,Saulo Marçal de; Souza,Gustavo; Carvalho,Marcelo Ayres; Simon,Marcelo F.; Viccini,Lyderson Facio.
Abstract Stylosanthes (Papilionoideae, Leguminosae) is a predominantly Neotropical genus with ~48 species that include worldwide important forage species. This study presents the chromosome number and morphology of eight species of the genus Stylosanthes (S. acuminata, S. gracilis, S. grandifolia, S. guianensis, S. hippocampoides, S. pilosa, S. macrocephala, and S. ruellioides). In addition, staining with CMA and DAPI, in situ hybridization with 5S and 35S rDNA probes, and estimation of DNA content were performed. The interpretation of Stylosanthes chromosome diversification was anchored by a comparison with the sister genus Arachis and a dated molecular phylogeny based on nuclear and plastid loci. Stylosanthes species showed 2n = 20, with low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachis; Cytogenetics; Evolution; Leguminosae; Stylosanthes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100306

Chromosomal characterization of Amazonian freshwater stingrays with evidence for new karyomorphs and XX/XY sex chromosomes
Provedor de dados: Genetics and Molecular Biology
Autores: Valentim,Francisco Carlos de Souza; Porto,Jorge Ivan Rebelo; Feldberg,Eliana.
Abstract Cytogenetic studies in the subfamily Potamotrygoninae have provided valuable insights into the understanding of the evolution and diversification of its species. In the present study, the chromosomal features of seven nominal potamotrygonin species are provided: Plesiotrygon iwamae (2n=74, FN=120), Potamotrygon amazona (2n=66, FN=107), P. constellata (2n=66, FN=110), P. leopoldi (2n=64, FN=102), P. motoro (2n=66, FN=106) from four different localities, and P. orbignyi (2n=66, FN=106), P. scobina (2n=66, FN=104), from Central Amazon. Additionally, we found a new karyomorph in P. wallacei. We considered the localization of Nucleolus Organizer Regions (NORs), as well as the pattern of constitutive heterochromatin, as species-specific characters. We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elasmobranchs; Cytogenetics; Chromosomal rearrangements; Sex chromosomes; Karyotypic diversity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400578

Chromosomal characterization of Pseudonannolene strinatii (Spirostreptida, Pseudonannolenidae)
Provedor de dados: Iheringia Série Zoologia
Autores: Campos,Kleber Agari; Fontanetti,Carmem S.
The chromosomes of the cave millipede Pseudonannolene strinatii Mauriès, 1974 were investigated. The diploid chromosome number was found to be 2n=16, XX/XY; the C-banding technique revealed a large amount of heterochromatin while the silver staining technique (Ag-NOR) evidenced the presence of heteromorphism of the NORs in some cells.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diplopoda; Cytogenetics; Caves; Heterochromatin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212004000100009

A new species of Urostreptus (Diplopoda, Spirostreptidae): description and chromossome number
Provedor de dados: Iheringia Série Zoologia
Autores: Pierozzi,Pedro H. B.; Fontanetti,Carmem S.
This work presents the description and chromosome number of Urostreptus atrobrunneus sp. nov. The genus until now had not been registered yet in the São Paulo State, Brazil. The meiotic analysis showed that the species presents 2n=24, XY. The C-banding revealed large blocks of constitutive heterochromatin and two heteromorphic chromosomal pairs, one of them corresponding to the sexual pair.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urostreptus; Diplopoda; Cytogenetics; Banding.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212006000200011

Caracterização citogenética de acessos de Brachiaria brizantha (Gramineae).
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: MENDES-BONATO, A. B.; VALLE, C. B. do; PAGLIARINI, M. S.; PENTEADO, M. I. de O.
No gênero Brachiaria, muitas espécies e acesso são poliplóides e apomíticos, o que dificulta o melhoramento via hibridização. Uma ampla caracterização citogenética, incluindo determinação do número de cromossomos e avaliação detalhada do comportamento meiótico, vem sendo realizada na coleção de germoplasma deste gênero a fim de auxiliar na seleção de genitores e explicar problemas na fecundidade dos acessos e híbridos do programa de melhoramento desenvolvido pela Embrapa Gado de Corte. Neste estudo, 22 acessos de Brachiaria brizantha foram analisados. Destes, um é diplóide (2n = 2x = 18), 18 são tetraplóides (2n = 6x = 54). Anormalidades meióticas variaram de 2,85% a 5,55% nas células em divisão no acesso diplóide e de 1,03% a 69,48% nos acessos...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Apomixia; Brachiaria Brizantha; Citogenética Vegetal; Cromossoma; Poliploidia.; Cytogenetics; Polyploidy.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/325562

Comportamento citológico atípico durante a microsporogênese em Brachiaria ruziziensis e B. decumbens.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: RISSO-PASCOTTO, C.; MENDES-BONATO, A. B.; PAGLIARINI, M. S.; VALLE, C. B. do.
Em gramíneas forrageiras do gênero Brachiaria, populações poliplóides naturais são abundantes e geralmente apomíticas, enquanto as diploídes, que se reproduzem sexualmente, são raras. Considerando-se a estreita correlação existente entre poloploidia e apomixia neste gênero, e a necessidade de identificação de genótipos sexuais compatíveis para o sucesso de hibridação, conhecimentos de características citológicas básicas como modo de reprodução, comportamento meiótico e nível de ploidia tornam-se essenciais para direcionar os programas de melhoramento. Durante análises desenvolvidas na Universidade Estadual de Maringá, com acessos de várias espécies de uma coleção de germoplasma, mantida na Embrapa Gado de Corte, três acessos, sendo um B....
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Braquiária; Feed grasses.; Brachiaria Decumbens; Brachiaria Ruziziensis; Citogenética Vegetal; Gramínea Forrageira; Meiose; Poliploidia.; Cytogenetics; Polyploidy.; Meiosis.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/324062

O capim-xaraés (Brachiaria brizantha cv. Xaraés) na diversificação de pastagens de braquiária.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: VALLE, C. B. do; EUCLIDES, V. P. B.; PEREIRA, J. M.; VALÉRIO, J. R.; PAGLIARINI, M. S.; MACEDO, M. C. M.; LEITE, G. G.; LOURENÇO, A. J.; FERNANDES, C. D.; DIAS FILHO, M. B.; LEMPP, B.; POTT, A.; SOUZA, M. A. de.
A cultivar Xaraés (Brachiaria brizantha), coletada em Burundi. África, foi liberada pela Embrapa em 2003 após 15 anos de avaliações. É uma planta cespitosa, de 1,5 m de altura, folha lanceolada e longa, com poucos pêlos e de coloração verde-escura. Os colmos são finos e radicantes nos nós, e as inflorescência são grandes, como espiguetas em uma só fileira. A cultivar Xaraés é apomítica e penteploíde, com 45 cromossomos. Irregularidades na divisão meiótica reduzem a validade do pólen para, aproximadamente, 79%. Seus principais atributos possitivos são a alta produtividade, especialmente de folhas, a rápida rebrota e o florescimento tardio, prolongado o período de pastejo até o período seco. Além disso, apresenta bom valor nutritivo e alta capacidade de...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Xaraés; Característica agronômica; Mata Atlântica; Brasil; Agronomic characters; Adaptation; Mixed pastures; Brazilian savannas.; Aclimatação; Brachiaria Brizantha; Citogenética; Cerrado; Diversificação de Cultura; Pastagem Cultivada; Plantio; Valor Nutritivo.; Brazil; Cytogenetics; Nutritive value; Planting; Sown pastures.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/326133

Obtention de souches conchylicoles performantes par polyploïdisation (5ème partie)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Baud, Jean-pierre; Nourry, Max; Haure, Joel; Heurtebise, Serge; Garnier, Jacqueline; Martin, Anne-genevieve; Claude, Serge; Langlade, Aime; Joly, Jean-pierre; Goulletquer, Philippe; Ruelle, Francois; Coatanea, Denis; Oheix, Jocelyne; Vercelli, Catherine; Chabirand, Jean-michel; Maurer, Daniele; Borel, Michel; Cardinal, Mireille; Cornet, Josiane; Le Pennec, Marcel; Le Mercier, A.
This fifth part of the polyploidisation program reports the experiments that were conducted in 1993 and that were devoted to : - monitoring growth performance of Ostrea edulis diploids and triploids in La Trinité sur Mer and Palavas - monitoring growth performance of Crassostrea gigas diploids and triploids in Port en Bessin, Marennes Oleron (2 locations), Arcachon and Palavas - comparative study of gametogenesis for C. gigas diploids and triploids - comparative study of C. gigas diploids and triploids using sensorial analysis At the end of fifth year, it was clear that triploids are advantageous although such a statement must be moderated according to locations. The gametogenesis stydy showed that triploidy is associated with partial to complete...
Tipo: Text
Palavras-chave: Crassostrea gigas; Ostrea edulis; Cytogenetics; Polyploidy; Crassostra gigas; Ostrea edulis; Cytogénétique; Polyploïdie.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/rapport-3860.PDF

Study of genetic damage in the japanese oyster induced by an environmentally-relevant exposure to diuron: evidence of vertical transmission of dna damage
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Barranger, Audrey; Akcha, Farida; Rouxel, Julien; Brizard, Raphael; Maurouard, Elise; Pallud, Marie; Menard, Dominique; Tapie, N.; Budzinski, Helene; Burgeot, Thierry; Benabdelmouna, Abdellah.
Pesticides represent a major proportion of the chemical pollutants detected in French coastal waters and hence a significant environmental risk with regards to marine organisms. Commercially-raised bivalves are particularly exposed to pollutants, among them pesticides, as shellfish farming zones are subject to considerable pressure from agricultural activities on the mainland.  The aims of this study were to determine (1) the genotoxic effects of diuron exposure on oyster genitors and (2) the possible transmission of damaged DNA to offspring and its repercussions on oyster fitness. To investigate these points, oysters were exposed to concentrations of diuron close to those detected in the Marennes-Oleron Basin (two 7-day exposure pulses at 0.4 and 0.6 μg...
Tipo: Text
Palavras-chave: Herbicide; Diuron; Oyster; Genotoxicity; Comet assay; Cytogenetics.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00162/27311/25541.pdf

Chromosome loss in bi-parental progenies of tetraploid Pacific oyster Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Mccombie, Helen; Lapegue, Sylvie; Cornette, Florence; Ledu, Christophe; Boudry, Pierre.
Alterations of chromosome number have been observed in the somatic tissue of Crassostrea gigas diploids and artificial polyploids. Tetraploid oysters with abnormal chromosome numbers in some or all of their tissue are considered undesirable as parents either for triploids (produced via a cross with diploids and of aquacultural interest) or for tetraploid breeding. Aneuploid tetraploid oysters may confer the tendency to lose chromosomes and to revert to lower ploidy levels to their offspring. More directly, their offspring could have a lower ploidy because of potential links between somatic and gametic chromosome loss. The present study evaluated the phenomenon in six bi-parental tetraploid families bred from parents of differing somatic ploidy quality. The...
Tipo: Text
Palavras-chave: Crassostrea gigas; Mitosis; Chromosome; Cytogenetics; Aneuploid; Polyptoid; Tetraploid; Oyster.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-633.pdf

Histology, cytogenetics and cytofluorymetry in diagnosis of neoplasia in Macoma balthica (Bivalvia, L.) from the southern Baltic Sea
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Katarzyna, S; Renault, Tristan; Wolowicz, M.
This study presents results of histopathologic, cytogenetics and flow cytometry analyses performed on Macoma balthica collected from the Gulf of Gdansk (Baltic Sea) in 2003 in order to compare the techniques for diagnosis of neoplasia. The proportion of affected clams gave a crude prevalence of 15.7 %. The four stages of the disease defined by histology and three stages of neoplasia defined by flow cytometry were reported. Stage I defined by flow cytometry corresponded to stages I and II defined by histology. Chromosome analysis did not lead to a staging of neoplastic progression. Both cytogenetics and flow cytometry indicated a difference in the DNA content of non-neoplastic and neoplastic cells. Cytogenetics examination marked that the range of...
Tipo: Text
Palavras-chave: Baltic Sea; Cytofluorymetry; Cytogenetics; Histology; Macoma balthica; Neoplasia.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2921.pdf

Cytogenetic study of Ostrea conchaphila (Mollusca : Bivalvia) and comparative karyological analysis within Ostreinae
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Leitao, Alexandra; Chaves, Raquel; Santos, Sara; Boudry, Pierre; Guedes Pinto, Henrique; Thiriot Quievreux, Catherine.
Chromosome preparations of the Olympia oyster Ostrea conchaphila Carpenter were studied using conventional Giemsa, silver staining, and C-banding techniques. The karyotype consists of six metacentric (1, 2, 4, 6, 8, and 10) and four submetacentric (3, 5, 7, 9) chromosome pairs. The silver-stained nucleolus organizer regions (Ag-NORs) were terminally located on the short arms of the submetacentric pair 5 (56% of cases) and on the long arms of submetacentric pair 7 (6% of cases). Constitutive heterochromatin was observed as telomeric C-bands on the short arm of the NOR-bearing chromosome pair 5 and as centromeric blocks of several chromosome pairs. Comparative analysis of patterns of karyotype, Ag-NORs, and C-bands of this species and of five other flat...
Tipo: Text
Palavras-chave: Ostreinae; Cytotaxonomy; Cytogenetics; Ostrea conchaphila.
Ano: 2002
URL: https://archimer.ifremer.fr/doc/2002/publication-2792.pdf

Neoplasia detection in Macoma balthica from the Gulf of Gdansk: comparison of flow cytometry, histology and chromosome analysis
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Smolarz, Kasia; Renault, Tristan; Soletchnik, Patrick; Wolowicz, Macciek.
A flow cytometry protocol was applied for the detection of neoplasia in Macoma balthica L. from the Gulf of Gdansk (Baltic Sea, Poland). A simple method, based on an osmotic shock, was used to permeabilise gill cells. The cytometric pattern of normal clams consisted of 2 peaks, a major peak B and a smaller peak C. The cytometric pattern of affected clams consisted of 2 peaks named B' and C'. Two parameters were used to define the stages of abnormalities in M. balthica clams based on the percentage of cells in peaks B, C, B' and C' and on the ratio between the fluorescence value of peaks B, C, B' and C' in all individuals. Three stages of neoplasia were clearly distinguished by flow cytometry considering peak C'. Stage 1 was characterised by a major...
Tipo: Text
Palavras-chave: Baltic Sea; Macoma balthica; Neoplasia; Cytogenetics; Histology; Flow cytometry.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2918.pdf

Cytogenetic Analysis of Ctenomys Opimus (Rodentia, Octodontidae) from Argentina
Provedor de dados: Mastozoología Neotropical
Autores: Toloza,Ariel C.; Scaglia,Orlando; Massarini,Alicia I.
Comparison of the normal karyotype, C and G-banding patterns of Ctenomys opimus from Argentina, with karyotypes from Chile and Bolivia, do not distinguish Argentinean from Chilean or Bolivian forms of this species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ctenomys opimus; Tuco-tucos; Cytogenetics; Argentina.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-93832004000100015

Discovery of the first wild population of the small red brocket deer Mazama bororo (Artiodactyla: Cervidae)
Provedor de dados: Mastozoología Neotropical
Autores: Vogliotti,Alexandre; Duarte,José M. B.
Mazama bororo was described from a few captive specimens in Brazil by cytogenetic and morphological characters. These specimens supposedly originated in the Southern Atlantic Forest; however, no wild population has been reported. This study was initiated in 1998 to investigate the presence of this species in forest remnants of the Paranapiacaba mountain range, south São Paulo State, Brazil. Five specimens were captured between 2000 and 2002. Cytogenetic analysis from blood samples confirmed its specific identification, documenting the first population of small red brocket deer at the Intervales State Park.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atlantic forest; Brazil; Cryptic deer species; Cytogenetics; Neotropical fauna.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-93832009000200027

Chromosomal differentiation in Kerodon rupestris (rodentia: caviidae) from the brazilian semi-arid region
Provedor de dados: Mastozoología Neotropical
Autores: Lessa,Gisele; Corrêa,Margaret M. O; Pessôa,Leila M; Zappes,Ighor A.
Kerodon rupestris (Wied, 1820) is the most specialized species among caviid rodents and is endemic to the extensive rocky outcrops in the semi-arid region in Brazil. Herein we describe different karyotypes for K. rupestris, based on samples collected in Itapajé, Ceará (CE), Iraquara in Bahia (BA), and Botumirim in Minas Gerais (MG). Our samples included northern, center and southern populations in the range of the species dis­tribution. The results were compared with a karyotype from Pernambuco previously described for the species. The diploid number (2n = 52) was constant among the analyzed population samples, but the fundamental number (FN) varied from 92 in Itapajé (CE) and Iraquara (BA), to 94 in Botumirim (MG). Pericentric inversions affecting one...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: C-Bands; Cytogenetics; Kerodon rupestris; NOR.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-93832013000200013

Chromosomal basis for a bilateral gynandromorph in Pycnogaster inermis (Rambur, 1838) (Orthoptera, Tettigoniidae)
Provedor de dados: Naturalis Digital Academic Repository
Autores: Barranco, P.; Cabrero, J.; Camacho, J.P.M.; Pascual, F.
Morphological and cytogenetic analyses of a spontaneous gynandromorph of Pycnogaster inermis, found in a natural population in the Spanish Sierra Nevada, have shown that the left side was male and the right side female, with almost perfect bilateral symmetry. Ventral valves of the ovipositor were very short, appearing as two spurs; dorsal valves were longer but still shorter than normal. The specimen had an apparently normal testis on the left side and a well-developed ovary on the right side. Cytogenetic analysis revealed the presence of 2n = 28 + X0 chromosomes in the testis and 2n = 28 + XX in the ovary, the same as standard males and females, respectively, of this species. This suggests that the gynandromorphism occurred at a very early developmental...
Tipo: Article / Letter to the editor
Palavras-chave: Gynandromorphism; Cytogenetics; Pycnogaster inermis; Tettigoniidae; Orthoptera.
Ano: 1995
URL: http://www.repository.naturalis.nl/record/504051

Morphological characterization, cytogenetic analysis, and geographical distribution of the Pygmy Marbled Newt Triturus marmoratus pygmaeus (Wolterstorff, 1905) (Caudata: Salamandridae)
Provedor de dados: Naturalis Digital Academic Repository
Autores: García-París, M.; Herrero, P.; Martín, C.; Dorda, J.; Esteban, M.; Arano, B.
Triturus marmoratus pygmaeus, a problematic subspecies of the Marbled Newt from the southern part of the Iberian Peninsula, is redescribed using specimens collected in the “typical” area. Diagnostic external morphological features are provided to permit the accurate determination of the specimens belonging either to T. m. marmoratus or to T. m. pygmaeus. These diagnostic features were applied to individuals both from the field and from museum collections. The results indicate a larger distributional area for T. m. pygmaeus than was previously recognized. The distribution of T. m. marmoratus ranges over the northern half of the Iberian Peninsula and most of France; T. m. pygmaeus occupies the southwestern part of the Iberian Peninsula. The contact area...
Tipo: Article / Letter to the editor
Palavras-chave: Taxonomy; Cytogenetics; Salamandridae; Triturus; Iberian Peninsula.
Ano: 1993
URL: http://www.repository.naturalis.nl/record/504012

Morphological characters and karyology of Miogryllus piracicabensis Piza (Orthoptera: Gryllidae)
Provedor de dados: Neotropical Entomology
Autores: Mesa,Alejo; García-Novo,Paula; Roat,Thaisa; Portugal,Cauré.
The genus Miogryllus includes a considerable number of species, most of them still undescribed. From those already described, the amount of afforded details on morphology is not always enough to recognize the species, missing information on phallic and pars stridens structures as well as karyology. The present paper aims to improve these information, on the species M. piracicabensis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gryllinae; Phallic sclerites; Par stridens; Proventiculus structure; Cytogenetics.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000300010

DNA barcode of Parodontidae species from the La Plata river basin - applying new data to clarify taxonomic problems
Provedor de dados: Neotropical Ichthyology
Autores: Bellafronte,Elisangela; Mariguela,Tatiane Casagrande; Pereira,Luiz Henrique Garcia; Oliveira,Claudio; Moreira-Filho,Orlando.
In the past years, DNA barcoding has emerged as a quick, accurate and efficient tool to identify species. Considering the difficulty in identifying some Parodontidae species from the La Plata basin and the absence of molecular data for the group, we aimed to test the effectiveness of DNA barcoding and discuss the importance of using different approaches to solve taxonomic problems. Eight species were analyzed with partial sequences of Cytochrome c oxidase I. The mean intraspecific K2P genetic distance was 0.04% compared to 4.2% for mean interspecific K2P genetic distance. The analyses of distance showed two pairs of species with K2P genetic divergence lower than 2%, but enough to separate these species. Apareiodon sp. and A. ibitiensis, considered as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apareiodon; COI gene; Cytogenetics; Fish identification; Morphology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300497

Karyotypic diversity between allopatric populations of the group Hoplias malabaricus (Characiformes: Erythrinidae): evolutionary and biogeographic considerations
Provedor de dados: Neotropical Ichthyology
Autores: Blanco,Daniel Rodrigues; Lui,Roberto Laridondo; Bertollo,Luiz Antonio Carlos; Margarido,Vladimir Pavan; Moreira Filho,Orlando.
Three populations of the group Hoplias malabaricus from the hydrographic basins of the São Francisco, Araguaia/Tocantins and Xingu Rivers in Brazil were analyzed using classic cytogenetic methods (Giemsa staining, C-banding and Ag-NORs) and molecular methods (fluorescent in situ hybridization with 18S rDNA, 5S rDNA and 5SHindIII satellite DNA probes). The chromosome markers allowed the characterization of these populations as belonging to karyomorph A and the detection of inter-population divergences. These differences likely stem from different evolutionary histories resulting from geographic isolation between populations associated to the dispersive mode of these organisms, reinforcing genetic diversity in the group Hoplias malabaricus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Trahira; Karyomorph A; FISH; Geographic isolation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252010000200015

Evidence of incipient speciation in Astyanax scabripinnis species complex (Teleostei: Characidae)
Provedor de dados: Neotropical Ichthyology
Autores: Castro,Jonathan P.; Moura,Maurício O.; Moreira-Filho,Orlando; Shibatta,Oscar A.; Santos,Mateus H.; Nogaroto,Viviane; Vicari,Marcelo R.; Almeida,Mara C. de; Artoni,Roberto F.
Two populations of the Astyanax scabripinniscomplex, isolated by a waterfall with over 100 meters depth and inhabiting different altitudes of the same river (1850 m a.s.l. and 662 m a.s.l.) were compared in reproductive data, geometric morphometry, tooth morphology, anal-fin rays counts, and karyotype, in order to test the hypothesis of speciation between the two populations. The results in the geometric morphometry analysis showed differences between the populations. Discriminant function analysis (DFA) and canonical variance analysis revealed sexual dimorphism. Secondary sexual characters, such as hooks in the anal fin rays of the males are absent in the lower altitude population. Both populations had the same macro karyotype structure, except for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allopatric populations; Cytogenetics; Evolution; Geometric morphometry; Paraíba do Sul River.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000200429

Chromosomal analyses in Megalonema platanum (Siluriformes: Pimelodidae), an endangered species from South American rivers
Provedor de dados: Neotropical Ichthyology
Autores: Carvalho,Rafael Augusto de; Sanchez,Sebástian; Swarça,Ana Claudia; Fenocchio,Alberto Sergio; Martins-Santos,Isabel C.; Dias,Ana Lúcia.
This study presents chromosomal data of Megalonema platanum from rio Tibagi, Paraná, Brazil and from rio Paraná, Argentina. The diploid number was equal 54 with karyotype composition of 24m+16sm+2st+12a in both populations. The AgNOR sites were detected in the terminal position of a submetacentric pair of the two analyzed populations, coinciding with secondary constrictions on the short arm of pair 15. CMA3 and FISH with 18S rDNA probe displayed fluorescent signals that correspond to the AgNOR sites and secondary constriction. The presence of a small acrocentric supernumerary chromosome can be observed in M. platanum from rio Tibagi, with centromeric heterochromatin. Others heterochromatic blocks were evidenced in the terminal position of some chromosome...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; C-banding; CMA3; FISH; NORs; Pimelodidae.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100017

Chromosomal analysis of Centromochlus heckelii (Siluriformes: Auchenipteridae), with a contribution to Centromochlus definition
Provedor de dados: Neotropical Ichthyology
Autores: Kowalski,Samantha; Paiz,Leonardo Marcel; da Silva,Maelin; Machado,Amanda de Souza; Feldberg,Eliana; Traldi,Josiane Baccarin; Margarido,Vladimir Pavan; Lui,Roberto Laridondo.
ABSTRACT Historically, there are divergences in the species allocation between Centromochlus and Tatia. This study aimed to generate the first cytogenetic data about Centromochlus and, by analyzing a population of Centromochlus heckelii from the Amazon River basin, to contribute as evidence to a historical taxonomic dilemma. Diploid number of 46 chromosomes and a heteromorphic pair was found in the female karyotypes, thus characterizing a ZZ/ZW sex chromosome system. Pale blocks of heterochromatin were located in centromeric regions of some chromosomes; however, the exclusive female chromosome (W) is almost entirely heterochromatic. AgNORs were detected in terminal position on the short arms of one acrocentric pair in males and two chromosome pairs in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Centromochlinae; Cytogenetics; Sex chromosome system; Taxonomy; ZZ/ZW.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000300203

Karyotypic and morphological divergence between two cryptic species of Eigenmannia in the Amazon basin with a new occurrence of XX/XY sex chromosomes (Gymnotiformes: Sternopygidae)
Provedor de dados: Neotropical Ichthyology
Autores: Silva,Danillo S.; Peixoto,Luiz A. W.; Pieczarka,Julio C.; Wosiacki,Wolmar B.; Ready,Jonathan S.; Nagamachi,Cleusa Y.
Eigenmannia species are widely distributed in the Neotropics, with eight valid species currently recognized. Populations of Eigenmannia from three locations in the eastern Amazon were investigated using cytogenetic and morphological techniques, revealing two taxa designated here as Eigenmannia sp. "A" and Eigenmannia sp. "B". The species differ in three morphometric characters, two meristic characters, and one osteological character. Eigenmannia sp. "A" presents 2n = 34 (22 m/sm+12 st/a) and Eigenmannia sp. "B" presents 2n = 38 (14 m/sm+24st/a) and simple differentiated sex chromosomes of the type XX/XY. In both species the Constitutive Heterochromatin (CH) rich in A-T bases is distributed in the centromeric region of all chromosomes. Eigenmannia sp. "B"...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; FISH; Knife fishes; Morphology; Sex chromosomes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000200297

Identification of distinct evolutionary units in allopatric populations of Hypostomus cf. wuchereri Günther, 1864 (Siluriformes: Loricariidae): karyotypic evidence
Provedor de dados: Neotropical Ichthyology
Autores: Bitencourt,Jamille de Araújo; Affonso,Paulo Roberto Antunes de Mello; Giuliano-Caetano,Lucia; Dias,Ana Lucia.
Few chromosomal reports are available for the endemic fish fauna from coastal basins in northeastern Brazil, and regional biodiversity remains partially or completely unknown. This is particularly true for Loricariidae, the most diverse family of armored catfishes. In the present work, allopatric populations of Hypostomus cf. wuchereri (Siluriformes: Loricariidae) from two basins in Bahia (northeastern Brazil) were cytogenetically analyzed. Both populations shared 2n = 76 chromosomes, a karyotype formula of 10m+18sm+48st/a (FN = 104) and single terminal GC-rich NORs on the second metacentric pair. Nevertheless, microstructural differences were detected by C-banding, fluorochrome staining and chromosomal digestion with restriction enzymes (Alu I, Bam HI,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity; Cytogenetics; Heterochromatin; Hypostominii.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000200008

Chromosomal variability in the wild ornamental species of Symphysodon (Perciformes: Cichlidae) from Amazon
Provedor de dados: Neotropical Ichthyology
Autores: Mesquita,Débora R.; Porto,Jorge I. R.; Feldberg,Eliana.
Cytogenetic studies were conducted on three discus species which inhabit the Amazon in Brazil: Symphysodon haraldi from Manacapuru, S. aequifasciatus from Tefé and S. discus from Barcelos. All individuals showed 2n=60 chromosomes, most of them biarmed. No sexual chromosomal heteromorphism was verified. However, different karyotypic formulae, owing to the presence of subtelocentric chromosomes, were verified for S. aequifasciatus and S. discus. One of the karyotypic formulae from S. aequifasciatus (cytotype 2) differs from the others, due to one of the homologues in the first chromosome pair being significantly larger than the other. A large variability was observed toward the nucleolar organizer regions (NOR) of S. haraldi and S. aequifasciatus. Although...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Discus; Cytogenetics; Polymorphism; Microchromosomes; Multiple NORs.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000200005

Chromosome analysis in Saccodon wagneri (Characiformes) and insights into the karyotype evolution of Parodontidae
Provedor de dados: Neotropical Ichthyology
Autores: Nirchio,Mauro; Masache,Maria Cecilia; Paim,Fabilene Gomes; Cioffi,Marcelo de Bello; Moreira Filho,Orlando; Barriga,Ramiro; Oliveira,Claudio; Rossi,Anna Rita.
ABSTRACT Parodontidae is a relatively small group of Neotropical characiform fishes consisting of three genera (Apareiodon, Parodon, and Saccodon) with 32 valid species. A vast cytogenetic literature is available on Apareiodon and Parodon, but to date, there is no cytogenetic data about Saccodon, a genus that contains only three species with a trans-Andean distribution. In the present study the karyotype of S. wagneri was described, based on both conventional (Giemsa staining, Ag-NOR, C-bands) and molecular (repetitive DNA mapping by fluorescent in situ hybridization) methods. A diploid chromosome number of 2n = 54 was observed in both sexes, and the presence of heteromorphic sex chromosomes of the ZZ/ZW type was detected. The W chromosome has a terminal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ag-NOR; Cytogenetics; FISH; Heterochromatin; ZW sex chromosomes.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100203

Cytogenetic and molecular analyses in troglobitic and epigean species of Pimelodella (Siluriformes: Heptapteridae) from Brazil
Provedor de dados: Neotropical Ichthyology
Autores: Dazzani,Bianca; Garcia,Caroline; Peixoto,Marilena; Trajano,Eleonora; Almeida-Toledo,Lurdes Foresti de.
Samples from seven different locations of the genus Pimelodella were genetically examined, two caves (exclusively subterranean, upper Tocantins River and São Francisco River) and five epigean (from upper Paraná River basin). Cytogenetic analyses revealed the same diploid number (2n=46) for all species besides similarities in both number and location of nucleolar organizer regions and C bands. FISH with 5S rDNA probes and CMA3 staining indicated significant differences among the studied species. Application of PCR-RFLP in ATPase 6 and 8 mitochondrial genes allowed building a minimum evolution phenogram identifying the close evolutionary relationship among groups. Both chromosomal and molecular data were useful to infer the relationships among studied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cave fish; Cytogenetics; Evolutionary relationships; FISH; PCR-RFLP.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000300016

A multi-approach analysis of the genetic diversity in populations of Astyanax aff. bimaculatus Linnaeus, 1758 (Teleostei: Characidae) from Northeastern Brazil
Provedor de dados: Neotropical Ichthyology
Autores: Pamponet,Vanessa de Carvalho Cayres; Carneiro,Paulo Luiz Souza; Affonso,Paulo Roberto Antunes de Mello; Miranda,Viviam Souto; Silva Júnior,Juvenal Cordeiro; Oliveira,Claudine Gonçalves de; Gaiotto,Fernanda Amato.
Few reports are available about the ichthyofauna of typical semi-arid rivers, although the regional diversity has been constantly threatened by human activities, mainly related to impoundment and construction of dams. The goal of the present work was to evaluate using different methods, the population genetic structure of a characin fish, Astyanax aff. bimaculatus, widespread throughout hydrographic basins of Bahia, Northeastern Brazil. Morphological (meristic and morphometric data), cytogenetic (karyotype and Ag-NOR), and molecular (RAPD and SPAR) analyses were carried out in specimens collected upstream and downstream of Pedra Dam, in the main channel of Contas River (Contas River Basin), and in the Mineiro stream, which belongs to the adjacent Recôncavo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphometry; Cytogenetics; RAPD; Population structure; Contas River.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000400010

Karyotype characterization and ZZ/ZW sex chromosome heteromorphism in two species of the catfish genus Ancistrus Kner, 1854 (Siluriformes: Loricariidae) from the Amazon basin
Provedor de dados: Neotropical Ichthyology
Autores: Oliveira,Renildo R. de; Feldberg,Eliana; Anjos,Maeda B. dos; Zuanon,Jansen.
We present karyotypic characteristics and report on the occurrence of ZZ/ZW sex chromosomes in Ancistrus ranunculus (rio Xingu) and Ancistrus sp. "Piagaçu" (rio Purus), of the Brazilian Amazon. Ancistrus ranunculus has a modal number of 2n=48 chromosomes, a fundamental number (FN) of 82 for both sexes, and the karyotypic formula was 20m+8sm+6st+14a for males and 19m+9sm+6st+14a for females. Ancistrus sp. "Piagaçu" presented 2n=52 chromosomes, FN= 78 for males and FN= 79 for females. The karyotypic formula was 16m+8sm+2st+26a for males and 16m+9sm+2st+25a for females. The high number of acrocentric chromosomes in karyotype of Ancistrus sp. "Piagaçu" differs from the majority of Ancistrini genera studied so far, and may have resulted from pericentric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypostominae; Ancistrini; Cytogenetics; Evolution; Heterochromatin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252007000300010

Cryptic species of the genus Pimelodella (Siluriformes: Heptapteridae) from the Miranda River, Paraguay River basin, Pantanal of Mato Grosso do Sul, Central Brazil
Provedor de dados: Neotropical Ichthyology
Autores: Souza-Shibatta,Lenice; Pezenti,Larissa Forim; Ferreira,Dhiego Gomes; Almeida,Fernanda Simões de; Sofia,Silvia Helena; Shibatta,Oscar Akio.
Specimens of Pimelodellacaptured in the Miranda River, Pantanal of Mato Grosso do Sul State, present morphological features that could indicate at least four species. Therefore, karyotype analysis and molecular biology provided evidence that they were only two species, one showing 2n = 46, and the other, 2n = 52 chromosomes, with only 18% genetic similarity. The morphological analysis evidenced that the dorsal filament is a male characteristic and that the upper lobe of the caudal fin was variable and might or might not be elongated in both species. With respect to morphometric characters, the formation of two groups was evident, but with a small overlap of specimens between them. Among the species with filaments on the dorsal fin observed in the Pantanal,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotaxonomy; Cytogenetics; Multivariate morphometrics; RAPD.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100101

Chromosome polymorphism in Ancistrus cuiabae Knaack, 1999 (Siluriformes: Loricariidae: Ancistrini)
Provedor de dados: Neotropical Ichthyology
Autores: Mariotto,Sandra; Centofante,Liano; Miyazawa,Carlos S.; Bertollo,Luiz Antonio Carlos; Moreira Filho,Orlando.
Cytogenetic and FISH analyses were performed in 30 Ancistrus cuiabae specimens from a bay near the town of Poconé, in the Pantanal of Mato Grosso, Brazil. The observed diploid number was 2n = 34 chromosomes for both sexes and three distinct katyotypic formulae were found, namely cytotype A (20m, 8sm, 6st, Fundamental Number/FN = 68; 6 males and 11 females), cytotype B (19m, 8sm, 6st, 1a, FN = 67; 8 males and 4 females) and cytotype C (18m, 8sm, 6st, 2a, FN = 66; a single male). NORs's analyses showed that these regions were located in distinct sites on the NOR-bearing chromosome pair, according to cytotypes. Thus, in cytotype A, NORs were located in the terminal region of the short arm of the second metacentric chromosome pair; in cytotype B, they were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; NOR; Pericentric inversion; Chromosomal evolution.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400006

Arachis hoehnei, the probable B genome donor of Arachis hypogaea based on crossability, cytogenetical and molecular studies
Provedor de dados: Open Acess Agriculture Research Repository
Autores: Mallikarjuna, N.
Palavras-chave: Genomes; Hybrids; Polyploidy; Cytogenetics; DNA; Groundnuts; Mosses; Genetic polymorphism; Polymorphism; Genetic improvement.
Ano: 2006
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3177

Use of 2n pollen in generating interspecific derivatives of groundnut
Provedor de dados: Open Acess Agriculture Research Repository
Autores: Mallikarjuna, N.
Palavras-chave: Genomes; Polyploidy; Nucleus; Hybrids; Chromosome number; Chromosomes; Mosses; Meiosis; Cytogenetics; DNA.
Ano: 2006
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3176

Abnormal meiotic behavior in three species of Crotalaria
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Ferreira,Kátia; Torres,Giovana Augusta; Carvalho,Isabelle Vilela de; Davide,Lisete Chamma.
The objective of this work was to compare the meiotic behavior and pollen grain viability of three species of Crotalaria. Slides for meiotic analysis were prepared by the air-drying technique. Pollen grain viability was measured by three staining procedures (Alexander's solution, tetrazolium chloride and fluorescein diacetate) and in vitro germination in a sucrose solution. Eight bivalents were observed, confirming previous reports on populations from other regions of Brazil, as well as from other countries. All species showed abnormal meiotic behavior as follows: in Crotalaria micans, cytomixis and abnormal chromosome pairing in diakinesis; in C. spectabilis, abnormal chromosome pairing in diplotene; in C. zanzibarica, shrunk nuclei in leptotene and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalaria; Cytogenetics; Cytomixis; Meiosis; Pollen grain viability.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001200012

Nuclear DNA content and chromosome number in Brachiaria spp. genotypes
Provedor de dados: Revista Ciência Agronômica (RCA)
Autores: Timbó,Ana Luiza de Oliveira; Pereira,Roselaine Cristina; Souza Sobrinho,Fausto; Davide,Lisete Chamma.
Breeding programs for Brachiaria spp. use both intraspecies and interspecies crosses between sexual and apomictic plants in order to obtain new cultivars with the desired characteristics. As there are different ploidy levels both within and between species of this genus, it becomes necessary to evaluate the genotypes used in breeding programs, as a guide to breeders when adopting crossing strategies. In this work, DNA content and chromosome number were determined in order to characterise ploidy levels in Brachiaria spp. genotypes. In the analysis of 15 genotypes, DNA content varied with the ploidy levels (2x, 3x and 4x), and between species and/or taxon. The average DNA content was 1.74 pg (2x) in B. ruziziensis, 3.74 pg (4x) in B. decumbens and 3.52 pg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germplasm bank; Cytogenetics; Flow cytometry; Forage.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000100008

New contributions to the study of Corixoidea: cytogenetic characterization of three species of Sigara from Argentina and the plausible mechanisms of karyotype evolution within Nepomorpha
Provedor de dados: Revista de la Sociedad Entomológica Argentina
Autores: Bressa,María José; Papeschi,Alba Graciela.
Cytogenetic studies in Heteroptera contribute to the analysis of evolutionary trends within the group. Heteroptera are characterized by the possession of holokinetic chromosomes, different sex chromosome mechanisms and a pair of m chromosomes in some species. In the present work, the male karyotype and meiosis in Sigara denseconscripta (Breddin), S. chrostowskii Jaczewski, and S. rubyae (Hungerford) are described. The three species share a diploid chromosome number of 2n= 24 with a pair of m chromosomes and an XY/XX sex chromosome system. With this study the chromosome number of 30 species of Corixoidea are known and the modal karyotype is 2n= 20+2m+XY in males. The available cytogenetic information in Heteroptera led us to suggest that the presence of a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Heteroptera; Holokinetic chromosomes; Meiosis; M chromosomes.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802007000200010

Meiotic behavior of interspecific hybrids between artificially tetraploidized sexual Brachiaria ruziziensis and tetraploid apomictic B. brizantha (Poaceae)
Provedor de dados: Scientia Agricola
Autores: Felismino,Mariana Ferrari; Pagliarini,Maria Suely; Valle,Cacilda Borges do.
The meiotic behavior of four interspecific promising hybrids was evaluated by conventional cytological methods. The female genitors were two artificially tetraploidized sexual accessions of B. ruziziensis (R41 and R44, 2n = 4χ = 36), which were crossed to an agronomically superior natural tetraploid apomictic genotype of B. brizantha (B140 - BRA003395). Three of them (HBGC313, HBGC 315, and HBGC324) were sexual and one (HBGC325) apomictic. Analyses of some cells in diakinesis revealed multivalent chromosome configurations, suggesting that genetic recombination and introgression of some genes could be present. The four hybrids had different types of meiotic abnormalities at various frequencies. Abnormalities related to irregular chromosome segregation due...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brachiaria; Brazil; Cytogenetics; Forage grasses; Meiosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000200010

Cytogenetic analysis of the Amazon stingless bee Melipona seminigra merrillae reveals different chromosome number for the genus
Provedor de dados: Scientia Agricola
Autores: Francini,Izaura Bezerra; Gross,Maria Claudia; Nunes-Silva,Carlos Gustavo; Carvalho-Zilse,Gislene Almeida.
Cytogenetic analysis of the Amazon stingless bee Melipona seminigra merrillae, by conventional Giemsa staining and C-banding, revealed a different chromosome number for Melipona: 2n = 22 for females and diploid drones while the haploid drones present n = 11. There is no evidence of B chromosomes. This result contrasts with previous studies, in which the chromosome number of 19 Melipona species was determined as 2n = 18 for females and n = 9 for haploid males. Based on cytogenetic information available for other Melipona species, we propose that M. s. merrillae has a more derived diploid number. This indicates that chromosome number is not a conservative characteristic within the genus as previously thought. Cytogenetic data for stingless bees are scarce,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meliponini; Neotropics; Cytogenetics; Diploid male chromosomes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000500012

Microsatellite diversity and chromosome number in natural populations of Trifolium riograndense Burkart
Provedor de dados: Scientia Agricola
Autores: Conterato,Ionara Fatima; Dall'Agnol,Miguel; Schifino-Wittmann,Maria Teresa; Janke,Aline.
Twenty eight natural populations of Trifolium riograndense Burkart, an important forage legume from native pastures of the state of Rio Grande do Sul, Brazil, were evaluated for genetic diversity with eight Simple Sequences Repeats (SSR) markers. Chromosome numbers were also determined. The eight markers were polymorphic, with 35 alleles and an average of 4.37 alleles per locus, and Polymorphism Information Content (PIC) between 0.48 and 0.80. Group analysis based on Jaccard´s similarity coefficient separated the 28 accessions in nine groups, with an average genetic similarity of 0.44, indicating a high genetic variability among the populations. No evident relation between genetic distance and geographical origin was detected. The chromosome number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Native clover; Genetic diversity; Cytogenetics; Native pastures.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000200005

Description and chromosome number of a species of Pseudonannolene Silvestri (Arthropoda, Diplopoda, Pseudonannolenidae)
Provedor de dados: Zoologia
Autores: Fontanetti,Carmem S.
Pseudonannolene mesai sp.n. from Biritiba Mirim, State of São Paulo, Brazil, is described and the chromosome number (2n=16) is reported. It was impossible to observe the chromosomal sex determination mechanism.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diplopoda; Pseudonannolene; Mtllipeds; Cytogenetics.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752000000100014

Cytogenetic analysis of Cycloramphus boraceiensis Heyer (Anura, Leptodactylidae)
Provedor de dados: Zoologia
Autores: Silva,Ana Paula Zampieri; Haddad,Célio F.B.; Kasahara,Sanae.
Cytogenetic studies on Cycloramphus boraceiensis Heyer, 1983 collected in Picinguaba, Ubatuba, State of São Paulo are presented. The species has 2n=26 karyotype formed by metacentrics, submetacentrics, and one telocentric pair which carries Ag-NORs. The C-banding patterns is also described.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anura; Cycloramphus; Chromosome; Cytogenetics.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000100011

First cytomolecular characterization of three Neotropical woody bamboos (Bambusoideae, Poaceae) suggests ancient diploidized karyotypes
Provedor de dados: Acta Botanica Brasilica
Autores: Zappelini,Julia; Souza,Luiz Gustavo; Guerra,Miguel Pedro; Pescador,Rosete.
ABSTRACT Although karyotype features are useful data for evolutionary studies, cytogenetic data in Bambusoideae are mainly based only on chromosome counts. The first comparative cytogenetic analysis of three Neotropical woody bamboo species - Guadua chacoensis, G. angustifolia and Chusquea tenella - was undertaken based on new and reviewed chromosome counts, CMA/DAPI double staining, fluorescent in situ hybridization (FISH) with 35S and 5S rDNA probes, and genome size estimation. The two species of Guadua were found to have 2n = 46 chromosomes, while the first record for C. tenella was 2n = 44. Only one pair of CMA+/DAPI- was detected on the terminal region of metacentric chromosomes in all three species. Likewise, one pair of 5S and 35S rDNA sites was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chusquea; Cytogenetics; Diploidization; Evolution; Guadua; Polyploidy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400673

Karyotypic characterization of Capsicum sp. accessions
Provedor de dados: Acta Scientiarum: Agronomy
Autores: Souza, Willame Rodrigues do Nascimento; Almeida, Angela Celis de; Carvalho, Reginaldo de; Ferreira, Regina Lúcia; Peron, Ana Paula.
This study aimed to determine the chromosome number and the karyotype of Capsicum annuum, Capsicum chinense, Capsicum frutencens and Capsicum baccatum accessions in the active Capsicum sp. genebank at the Federal University of Piauí (BGC-UFPI). These species have great economic importance throughout the world, and their cytogenetic characterization can inform taxonomy and lead to improvement in the genus. Karyotypes were obtained from the rootlet meristems of the studied accessions using the squash method and Giemsa staining. The chromosome number 2n=2x=24 was verified for each of the four species. Chromosomal polymorphisms were observed for the C. frutencens accession BGC 37, which presented 12 pairs of metacentric chromosomes instead of 11 pairs of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CITOGENÉTICA pepper; Cytogenetics; Genebank; Morphometry CITOGENÉTICA DE CULTIVARES DE INTERESSE ECONÔMICO.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19485

Número cromossômico em acessos de Pennisetum spp. (Poaceae, Poales) - DOI: 10.4025/actascibiolsci.v30i3.5016
Provedor de dados: Acta Scientiarum: Biological Sciences
Autores: Techio, Vânia Helena; UNC; Davide, Lisete Chamma; UFLA; Souza, Tatiana Matioli; 3Bios Consultoria e Serviços Ambientais Ltda; Pereira, Antônio Vander; EMBRAPA-MG.
Avaliou-se o complemento cromossômico de 16 acessos de Pennisetum disponíveis na Coleção de Germoplasma da Embrapa Gado de Leite – Juiz de Fora, Estado de Minas Gerais. Para a avaliação citogenética, utilizou-se a técnica de esmagamento e coloração com Feulgen. Para quatro acessos foram obtidas metáfases com 2n = 28 cromossomos, confirmando sua classificação como P. purpureum. A origem híbrida P. purpureum x P. glaucum foi constatada em oito acessos, os quais apresentaram 2n = 21. Em um acesso identificado como híbrido entre P. purpureum x P. glaucum, foram observadas metáfases com 2n = 54, permitindo inferir que não se trata de um híbrido interespecífico triplóide. Para outros três acessos avaliados, também foram observadas células com 2n = 54, embora...
Palavras-chave: 2.00.00.00-6    Ciências Biológicas Pennisetum; Citogenética; Germoplasma; Híbridos interespecíficos. Pennisetum; Cytogenetics; Germplasm; Interspecific hybrids.
Ano: 2008
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5016

Cytogenetic damage related to low levels of methyl mercury contamination in the Brazilian Amazon
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: AMORIM,MARÚCIA I. M.; MERGLER,DONNA; BAHIA,MARCELO O.; DUBEAU,HÉLÈNE; MIRANDA,DANIELA; LEBEL,JEAN; BURBANO,ROMMEL R.; LUCOTTE,MARC.
The mercury rejected in the water system, from mining operations and lixiviation of soils after deforestation, is considered to be the main contributors to the contamination of the ecosystem in the Amazon Basin. The objectives of the present study were to examine cytogenetic functions in peripheral lymphocytes within a population living on the banks of the Tapajós River with respect to methylmercury (MeHg) contamination, using hair mercury as a biological indicator of exposure. Our investigation shows a clear relation between methylmercury contamination and cytogenetic damage in lymphocytes at levels well below 50 micrograms/gram, the level at which initial clinical signs and symptoms of mercury poisoning occur. The first apparent biological effect with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian Amazon; Mercury; Mitotic Index; Cytogenetics.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000400004

Karyotypic analysis in species of the genus Dasyprocta (Rodentia: Dasyproctidae) found in Brazilian Amazon
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: RAMOS,ROSEMAR S. L.; VALE,WILLIAM G.; ASSIS,FÁTIMA L.
A total of 30 animals of the genus Dasyprocta were cytogenetically studied. They belong to the following species: D. prymnolopha (N=20), D. leporina (N=6), D. fuliginosa (N=1) and Dasyprocta sp. (N=3) (Dasyproctidae, Hystricognathi). Cell suspensions were obtained by peripheral blood culture, besides bone marrow and spleen cells, from D. prymnolopha and D. leporina. The diploid number was 64/65 for all samples. The karyotypes showed similarity, and chromosomal polymorphism was not detected by Giemsa conventional staining and G banding. The constitutive heterochromatin distribution at the pericentromeric region of all the chromosomes was similar in all species. D. prymnolopha, D. leporina and Dasyprocta sp. presented variation in the heterochromatical block...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Hystricognathi; Dasyprocta; Karyotype.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000100007

Evidence of separate karyotype evolutionary pathway in Euglossa orchid bees by cytogenetic analyses
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: FERNANDES,ANDERSON; WERNECK,HUGO A.; POMPOLO,SILVIA G.; LOPES,DENILCE M.
Euglossini are solitary bees considered important pollinators of many orchid species. Information regarding chromosome organization is available for only a small number of species in this group. In the present work, the species Euglossa townsendi and E. carolina were analyzed by cytogenetic techniques to collect information that may aid the understanding of their evolution and chromosomal organization. The chromosome number found was n = 21 for males and 2n = 42 for females in the two species. The distribution and amount of heterochromatin regions differed in the two species analyzed, where they were classified as “high” or “low” heterochromatin content, similarly to what has already been performed in social bee species of the genus Melipona. Banding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Euglossini; Heterochromatin; Karyotypic evolution.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000300937

Karyotype characterization and nuclear DNA content measurement in Bromeliaceae: State of the art and future perspectives
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: NUNES,ANDREI C.P.; CLARINDO,WELLINGTON R.
In Bromeliaceae, cytogenetic and flow cytometry analyses have been performed to clarify systematic and evolutionary aspects. Karyotyping approaches have shown the relatively high chromosome number, similar morphology and small size of the chromosomes. These facts have prevented a correct chromosome counting and characterization. Authors have established a basic chromosome number of x = 25 for Bromeliaceae. Recently, one karyomorphological analysis revealed that x = 25 is no longer the basic chromosome number, whose genome may have a polyploid origin. Besides cytogenetic characterization, the 2C DNA content of bromeliads has been measured. Nuclear DNA content has varied from 2C = 0.60 to 2C = 3.34 picograms. Thus, in relation to most angiosperms, the 2C DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allopolyploid; Bromeliads; Cytogenetics; Flow cytometry; Genome evolution.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401849

Chromosome comparison between populations of the collared peccary, Tayassu tajacu, raised in captivity
Provedor de dados: Biocell
Autores: Carvalho de Souza,Patrícia; Salim Khayat,André; Chamon Seligmann,Igor; Rodríguez Burbano,Rommel Mario.
The collared peccary (Tayassu tajacu) is widely distributed over the American continent, being found from the south of the USA to the north of Argentina. In Brazil, it is spread all over the country, being one of the potential species to be raised in captivity. Therefore, the cytogenetic techniques could be a potential tool for reproductive monitoring of animals raised in captivity, mainly when destined for commercial purposes. This study had the objective of determining the chromosome number of two populations raised in captivity and characterizing them by GTG banding. For this purpose, an analysis was made of mitotic metaphases obtained from lymphocyte cultures made from blood samples of 11 animals, six of which from the Northeast and five from the North...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytogenetics; GTG Banding; Tayassuidae; Suidae; Pigs.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200007

Polytypic and polymorphic cytogenetic variations in the widespread anuran Physalaemus cuvieri (Anura, Leiuperidae) with emphasis on nucleolar organizing regions
Provedor de dados: Biological Research
Autores: QUINDERÉ,YEDA R. S. D; LOURENÇO,LUCIANA B; ANDRADE,GILDA V; TOMATIS,CRISTIAN; BALDO,DIEGO; RECCO-PIMENTEL,SHIRLEI M.
We investigated the NOR distribution in ten populations of Physalaemus cuvieri from different regions of Brazil and Argentina. A high variability in NOR pattern was observed and provided a useful tool in grouping several populations. The specimens from the state of Tocantins, northern Brazil, could easily be distinguished from all the other analyzed populations, since its karyotype presented NORs in the chromosome pairs 1,3,4 and 10 (and sometimes also in chromosome 5), and several pericentromeric C-bands. A NOR-site in chromosome 9 characterized three populations from the northeastern region of Brazil. Interestingly, the P. cuvieri populations located in opposite extremes of the geographic distribution had, as a fixed condition, the presence of NORs in 8q...
Tipo: Journal article
Palavras-chave: Anura; Chromosome; Cytogenetics; NOR; Physalaemus; Polymorphic variations; Polytypic variations.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100008

Cytogenetics of the freshwater cyclopoid mesocyclops longisetus longisetus (crustacea, copepoda) from São Carlos, São Paulo, Brazil
Provedor de dados: Biota Neotropica
Autores: Silva,William Marcos da; Tundisi,Takako Matsumura.
A cytogenetic study was carried out on the early embryonic stages of the cyclopoid species Mesocyclops longisetus longisetus (Crustacea, Copepoda) from freshwater environments of the São Carlos area of south-east Brazil. Chromatin was staining using acetic orcein solution. The species showed 2n = 14 chromosomes and presented chromatin diminution in the 4th embryonic cleavage. The eliminated chromatin was concentrated in the equatorial region of the embryonic cell. The results were compared with data for other cyclopoid species of temperate regions.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cyclopoida; Mesocyclops longisetus longisetus; Cytogenetics; Chromatin diminution; Chromosomal number.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032004000200019

Biosystematic and chemosystematic studies in five South American species of Conyza (Asteraceae)
Provedor de dados: Boletín de la Sociedad Argentina de Botánica
Autores: Urdampilleta,Juan D.; Amat,Aníbal G.; Bidau,Claudio J.; Kolb Koslobsky,Nicolás.
The composition of the essentials oils of five species of Conyza Less from Argentina was determined by Gas Chromatography-Mass Spectrometer. This composition is associated to morphological and cytogenetic characters. The monoterpenes constitute more than 60% of the essential oils in C. blakei, C glandulitecta, C. sumatrensis var. sumatrensis and C. sumatrensis var. floribunda, in which limonene is the predominant compound. In C. bonariensis and C. primulaefolia the monoterpene content constitute less than the 40%. C. bonariensis presents only 13% limonene, while in C. primulaefolia it is absent. The similarity analysis of monoterpenes showed a relationship between the morphological and cytogenetic analysis, and the monoterpene content character seems to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essential oils; Chemosystematics; Morphology; Cytogenetics; Conyza; Asteraceae.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722005000100011

Cytotaxonomy, heterochromatic polymorphism and natural triploidy of a species of Astyanax (Pisces, Characidae) endemic to the Iguaçu river basin
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Kantek,Daniel Luis Zanella; Noleto,Rafael Bueno; Fenocchio,Alberto Sérgio; Cestari,Marta Margarete.
Cytogenetic analysis with Astyanax sp. D revealed a karyotype of 2n=50 with 2M+26SM+6ST+16A, besides a triploid specimen showing 2n=75 chromosomes (3M+39SM+9ST+24A). C-banding strongly stained the terminal regions of several SM-ST-A chromossomes. Two pairs of acrocentric chromosomes presented interstitial heterochromatin, this state being polymorphic and occuring due to possible paracentric inversions. The results obtained with the AluI restriction enzyme and A3 chromomycin were similar to the C-banding. Relationships were proposed between Astyanax sp. D and A. scabripinnis, as well as considerations for a possible origin of the triploid specimen (2n=3x=75). When comparing the present results with cytogenetic features of other endemic Astyanax species in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Triploidy; Paracentric inversion.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100008

Cytogenetics of six Brazilian species of Psocoptera
Provedor de dados: Brazilian Journal of Genetics
Autores: Zefa,Edison; Fontanetti,Carmem Silvia; Mesa,Alejo.
The karyotypes of the following six species of Brazilian Psocoptera are reported: Caecillius sp. (Caecillidae), Triplocania ? caudata New (Ptiloneuridae), Brachinodiscus cf. lepidus (Banks) (Psocidae), Psococerastis interrupta New (Psocidae), Ptycta nr reticulata New (Psocidae) and Trichadenotecnum sinuatum New (Psocidae). All of them had males with 2n = 17 and an XO sex determining mechanism.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Psocoptera.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400011

Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Santos,M.F.M.; Oliveira,F.C.A.C.; Kishimoto,R.K.; Borri,D.; Santos,F.P.S.; Campregher,P.V.; Silveira,P.A.A.; Hamerschlak,N.; Mangueira,C.L.P.; Duarte,F.B.; Crepaldi,A.H.; Salvino,M.A.; Velloso,E.D.R.P.
Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Karyotype; Leukemia; Myelodysplasia; Myeloproliferative disease.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200608

Microsatellite instability and cytogenetic survey in myeloid leukemias
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ribeiro,E.M.S.F.; Rodriguez,J.M.; Cóser,V.M.; Sotero,M.G.; Fonseca Neto,J.M.; Pasquini,R.; Cavalli,I.J.
Microsatellites are short tandem repeat sequences dispersed throughout the genome. Their instability at multiple genetic loci may result from mismatch repair errors and it occurs in hereditary nonpolyposis colorectal cancer. This instability is also found in many sporadic cancers. In order to evaluate the importance of this process in myeloid leukemias, we studied five loci in different chromosomes of 43 patients, 22 with chronic myelocytic leukemia (CML) in the chronic phase, 7 with CML in blast crisis, and 14 with acute myeloid leukemia (AML), by comparing leukemic DNA extracted from bone marrow and constitutional DNA obtained from buccal epithelial cells. Only one of the 43 patients (2.1%), with relapsed AML, showed an alteration in the allele length at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genomic instability; Mismatch repair errors; Cytogenetics; Leukemogenesis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200003

Somatic cytogenetic and azoospermia factor gene microdeletion studies in infertile men
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pina-Neto,J.M.; Carrara,R.C.V.; Bisinella,R.; Mazzucatto,L.F.; Martins,M.D.; Sartoratto,E.; Yamasaki,R.
The objective of the present study was to determine the frequency of somatic chromosomal anomalies and Y chromosomal microdeletions (azoospermia factor genes, AZF) in infertile males who seek assisted reproduction. These studies are very important because the assisted reproduction techniques (mainly intracytoplasmic sperm injection) bypass the natural selection process and some classical chromosomal abnormalities, microdeletions of AZF genes or some deleterious genic mutations could pass through generations. These genetic abnormalities can cause in the offspring of these patients male infertility, ambiguous external genitalia, mental retardation, and other birth defects. We studied 165 infertile men whose infertility was attributable to testicular problems...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Y microdeletions; Male infertility; Karyotype; Chromosome aberrations.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400017

Cytogenetic studies of Brazilian pediatric myelodysplastic syndrome cases: challenges and difficulties in a large and emerging country
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Velloso,E.D.R.P.; Chauffaille,M.L.; Peliçario,L.M.; Tanizawa,R.S.S.; Toledo,S.R.C.; Gaiolla,R.D.; Lopes,L.F.
Myelodysplastic syndromes (MDS) and juvenile myelomonocytic leukemia (JMML) are rare hematopoietic stem cell diseases affecting children. Cytogenetics plays an important role in the diagnosis of these diseases. We report here the experience of the Cytogenetic Subcommittee of the Brazilian Cooperative Group on Pediatric Myelodysplastic Syndromes (BCG-MDS-PED). We analyzed 168 cytogenetic studies performed in 23 different cytogenetic centers; 84 of these studies were performed in patients with confirmed MDS (primary MDS, secondary MDS, JMML, and acute myeloid leukemia/MDS+Down syndrome). Clonal abnormalities were found in 36.9% of the MDS cases and cytogenetic studies were important for the detection of constitutional diseases and for differential diagnosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Childhood myelodysplastic syndromes; Juvenile myelomonocytic leukemia; Cytogenetics; Karyotype; Diagnosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100085