Consulta: subjectFacets:"Insulin"
Registros recuperados: 63
Data/hora: 09/06/2024 06:40:32

Influencia de la nutrición en los programas de sincronización de estros, superovulación y transferencia embrionaria en ovejas.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Molina Mendoza, Pedro.
El aspecto nutricional y endocrino tiene influencia en la sincronización de estros y programas de superovulación, evaluándose su efecto en dos experimentos. En el experimento 1 se evaluaron dos régimenes alimenticios y dos programas de sincronización de estros, teniendo los siguientes tratamientos: ovejas sin pre-sincronización alimentadas con heno de avena (HS) o concentrado comercial (TS) y pre-sincronizadas con heno de avena (HP), o concentrado comercial (TP). En el experimento 1 la concentración de P4 fue mayor en  grupos HP y TP (P&lt;0.05). El inicio del estro y la elevación de LH fueron más rápido en los grupos HS y HP (P&lt;0.05). En el experimento 2 se evaluaron dos régimenes de alimentación, un grupo alimentado con concentrado comercial (T) y...
Palavras-chave: Ovis aries; Grasa dorsal; Superovulación; Progesterona; Insulina; Backfat; Superovulation; Progesterone; Insulin; Doctorado; Ganadería.
Ano: 2010
URL: http://hdl.handle.net/10521/96

Efecto antidiabético de los polisacáridos del fruto de xoconostle (Opuntia matudae) en ratas.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Calvo Arriaga, Ayne Oliva.
La diabetes mellitus afecta a más de 220 millones de personas en el mundo y es una de las causas principales de muerte. Existe evidencia de que los frutos ácidos de Opuntia joconostle y O. matudae, denominados xoconostle, disminuyen la glucemia e incrementan la concentración sérica de insulina en individuos diabéticos. El presente estudio fue propuesto para dilucidar si esos efectos y otros relacionados con el metabolismo energético, como la disminución de colesterol y triglicéridos en la sangre, se deben a los mucílagos, pectinas o hemicelulosas de xoconostle, a todos ellos o a ninguno de ellos. La hipótesis es que estos polisacáridos ocasionan disminución de las concentraciones séricas de glucosa, colesterol y triglicéridos, e incremento de la secreción...
Palavras-chave: Dislipidemia; Fibra alimentaria; Glucemia; Insulina; Dietary fiber; Dyslipidemia; Glycemia; Insulin; Maestría; Botánica.
Ano: 2011
URL: http://hdl.handle.net/10521/526

Grasa de sobrepaso en ovejas con diferente  espesor de grasa dorsal y su respuesta en el estro sincronizado, perfil endocrino, porcentaje de gestación y prolificidad.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Nieto Aquino, Rafael.
El objetivo de este estudio fue evaluar el efecto de la grasa de sobrepaso en el estro  sincronizado, porcentaje de gestación y prolificidad en ovejas con baja y alta grasa  dorsal, y su respuesta en hormona luteinizante (LH), estradiol (E2), progesterona (P4) e  insulina (INS). Se utilizaron 59 ovejas, con diferente espesor de grasa dorsal, bajo  (EGb) y alto (EGa), que se subdividieron en grupos: sin (EGbSg, n = 16) y con (EGbCg,  n = 14) la adición de 150 g de grasa de sobrepaso; y sin (EGaSg, n = 14) y con  (EGaCg, n = 15) la adición de 150 g de grasa de sobrepaso, respectivamente. Se  sincronizaron con esponjas de FGA (20 mg), por 12 d, a diez días de su inserción se  aplicaron 15 mg de PGF2α. No se encontraron diferencias (P &gt; 0.05) para las...
Palavras-chave: Ultrasonografía; Insulina; Progesterona; Estradiol; Hormona luteinizante. Ultrasonagraphy; Insulin; Progesterone; Estradiol; Luteinizing hormone.
Ano: 2012
URL: http://hdl.handle.net/10521/882

Efecto antidiabético de los polisacáridos del fruto de xoconostle (Opuntia matudae) en ratas.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Calvo Arriaga, Ayne Oliva.
La diabetes mellitus afecta a más de 220 millones de personas en el mundo y es una de las causas principales de muerte. Existe evidencia de que los frutos ácidos de Opuntia joconostle y O. matudae, denominados xoconostle, disminuyen la glucemia e incrementan la concentración sérica de insulina en individuos diabéticos. El presente estudio fue propuesto para dilucidar si esos efectos y otros relacionados con el metabolismo energético, como la disminución de colesterol y triglicéridos en la sangre, se deben a los mucílagos, pectinas o hemicelulosas de xoconostle, a todos ellos o a ninguno de ellos. La hipótesis es que estos polisacáridos ocasionan disminución de las concentraciones séricas de glucosa, colesterol y triglicéridos, e incremento de la secreción...
Palavras-chave: Dislipidemia; Fibra alimentaria; Glucemia; Insulina; Dietary fiber; Dyslipidemia; Glycemia; Insulin; Maestría; Botánica.
Ano: 2011
URL: http://hdl.handle.net/10521/526

Grasa de sobrepaso en ovejas con diferente  espesor de grasa dorsal y su respuesta en el estro sincronizado, perfil endocrino, porcentaje de gestación y prolificidad.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Nieto Aquino, Rafael.
El objetivo de este estudio fue evaluar el efecto de la grasa de sobrepaso en el estro  sincronizado, porcentaje de gestación y prolificidad en ovejas con baja y alta grasa  dorsal, y su respuesta en hormona luteinizante (LH), estradiol (E2), progesterona (P4) e  insulina (INS). Se utilizaron 59 ovejas, con diferente espesor de grasa dorsal, bajo  (EGb) y alto (EGa), que se subdividieron en grupos: sin (EGbSg, n = 16) y con (EGbCg,  n = 14) la adición de 150 g de grasa de sobrepaso; y sin (EGaSg, n = 14) y con  (EGaCg, n = 15) la adición de 150 g de grasa de sobrepaso, respectivamente. Se  sincronizaron con esponjas de FGA (20 mg), por 12 d, a diez días de su inserción se  aplicaron 15 mg de PGF2α. No se encontraron diferencias (P &gt; 0.05) para las...
Palavras-chave: Ultrasonografía; Insulina; Progesterona; Estradiol; Hormona luteinizante. Ultrasonagraphy; Insulin; Progesterone; Estradiol; Luteinizing hormone.
Ano: 2012
URL: http://hdl.handle.net/10521/815

Influencia de la nutrición en los programas de sincronización de estros, superovulación y transferencia embrionaria en ovejas.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Molina Mendoza, Pedro.
El aspecto nutricional y endocrino tiene influencia en la sincronización de estros y programas de superovulación, evaluándose su efecto en dos experimentos. En el experimento 1 se evaluaron dos régimenes alimenticios y dos programas de sincronización de estros, teniendo los siguientes tratamientos: ovejas sin pre-sincronización alimentadas con heno de avena (HS) o concentrado comercial (TS) y pre-sincronizadas con heno de avena (HP), o concentrado comercial (TP). En el experimento 1 la concentración de P4 fue mayor en  grupos HP y TP (P&lt;0.05). El inicio del estro y la elevación de LH fueron más rápido en los grupos HS y HP (P&lt;0.05). En el experimento 2 se evaluaron dos régimenes de alimentación, un grupo alimentado con concentrado comercial (T) y...
Palavras-chave: Ovis aries; Grasa dorsal; Superovulación; Progesterona; Insulina; Backfat; Superovulation; Progesterone; Insulin; Doctorado; Ganadería.
Ano: 2010
URL: http://hdl.handle.net/10521/96

Grasa de sobrepeso en ovejas con diferente  espesor de grasa dorsal y su respuesta en el estro sincronizado, perfil endocrino, porcentaje de gestación y prolificidad.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Nieto Aquino, Rafael.
El objetivo de este estudio fue evaluar el efecto de la grasa de sobrepaso en el estro  sincronizado, porcentaje de gestación y prolificidad en ovejas con baja y alta grasa  dorsal, y su respuesta en hormona luteinizante (LH), estradiol (E2), progesterona (P4) e  insulina (INS). Se utilizaron 59 ovejas, con diferente espesor de grasa dorsal, bajo  (EGb) y alto (EGa), que se subdividieron en grupos: sin (EGbSg, n = 16) y con (EGbCg,  n = 14) la adición de 150 g de grasa de sobrepaso; y sin (EGaSg, n = 14) y con  (EGaCg, n = 15) la adición de 150 g de grasa de sobrepaso, respectivamente. Se  sincronizaron con esponjas de FGA (20 mg), por 12 d, a diez días de su inserción se  aplicaron 15 mg de PGF2α. No se encontraron diferencias (P &gt; 0.05) para las...
Tipo: Tesis
Palavras-chave: Ultrasonografía; Insulina; Progesterona; Hormona luteinizante.; Maestría; Ganadería; Ultrasonagraphy; Insulin; Progesterone; Estradiol; Luteinizing hormone.
Ano: 2009
URL: http://hdl.handle.net/10521/1210

Protective Effect of Lepidium sativum Seed Extract on Histopathology and Morphology of Epididymis in Diabetic Rat Model
Provedor de dados: International Journal of Morphology
Autores: Kamani,Mehran; Hosseini,Elahe Seyed; Kashani,Hamed Haddad; Atlasi,Mohammad Ali; Nikzad,Hossein.
Diabetes mellitus is a frequent and serious metabolic illness all over the world and plants have been a desirable source of medicine recently. Diabetes has unpleasant effect on male reproductive system and it may lead to male infertility. It causes erectile dysfunction and reduces ejaculate volume by affecting the health of small blood vessels and the small nerves that control ejaculation and also decreases libido by decreasing testosterone levels. Current study evaluated the possible protective efficiency of Lepidium sativum (Garden cress) seed extract on fasting blood sugar (FBS) and then assessed histopathological change of epididymis in streptozotocine (STZ)-induced diabetic rats. We randomly categorized 50 adult male Wistar rats into five groups (each...
Tipo: Journal article
Palavras-chave: Diabetes; Epididymis; Lepidium sativum (garden cress); Streptozotocine; Insulin.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200036

SCORPION ANTIVENOM REVERSES METABOLIC, ELECTROCARDIOGRAPHIC, AND HORMONAL DISTURBANCES CAUSED BY THE INDIAN RED SCORPION Mesobuthus tamulus concanesis, Pocock ENVENOMATION
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: MURTHY,K. RADHA KRISHNA; ZARE,M. ABBAS.
Electrocardiographic (ECG) changes were induced in dogs by injection of scorpion venom from Mesobuthus tamulus concanesis, Pocock. Venom (3 mg/kg body weight) was given subcutaneously (SQ) while 10 ml of scorpion antivenom (SAV) was administered intravenously (IV) to experimental dogs. Group 1 received only the venom; Groups 2, 3, and 4 received SAV at 0, 30, and 60 min, respectively, following envenoming. Thick, ropy and profuse salivation; muscle fasciculation; clonus and tetany-like contractions; frequent urination; and bowel emptying sometimes stained with bile and occasionally blood and bile were observed 20-25 minutes after envenoming. Following envenoming, hyperglycemia, increase in free fatty acid (FFA) levels, and reduction in triglyceride levels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antivenom; Scorpion venom; Mesobuthus tamulus concanesis; Hyperglycemia; Free fatty acids; Triglycerides; Insulin; Hyperinsulinemia; Insulin resistance; Electrocardiographic changes.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100004

The scorpion envenoming syndrome: a different perspective. The physiological basis of the role of insulin in scorpion envenoming
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: MURTHY,K. R. KRISHNA.
Death caused by scorpion envenoming (Buthidae family) is a common event in tropical and subtropical countries. Severe scorpion envenoming causes an autonomic storm resulting in a massive release of catecholamines, angiotensin II, glucagon, cortisol, and changes in insulin secretion. As a consequence of these changes in the hormonal milieu, scorpion envenoming results in a syndrome of fuel energy deficits and an inability of the vital organs to utilize the existing metabolic substrates, which causes myocardial damage, cardiovascular disturbances, peripheral circulatory failure, pulmonary oedema, and many other clinical manifestations alone or in combination, producing multi-system-organ-failure (MSOF) and death. Insulin-glucose infusion or antivenom...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catecholamines; Angiotensin II; Glucagon; Cortisol; Insulin; Myocardial damage; Cardiovascular disturbances; Peripheral circulatory failure; Pulmonary oedema; Multi-system-organ-failure (MSOF).
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302000000100002

THE BLOOD LEVELS OF GLUCAGON, CORTISOL, AND INSULIN FOLLOWING THE INJECTION OF VENOM BY THE SCORPION (Mesobuthus tamulus concanesis, POCOCK) IN DOGS
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: RADHA KRISHNA MURTHY,K.; HAGHNAZARI,L.
Severe envenoming was induced in two groups of experimental dogs after subcutaneous (SQ) injection of venom of the scorpion (Mesobuthus tamulus concanesis, Pocock) (3.0 and 3.5 mg/kg body weight). The circulating levels of blood sugar, insulin, glucagon, and cortisol were assayed at 0, and 30, 60, 90 and 120 min after venom injection. There was an increase in the circulating levels of blood sugar, insulin, glucagon, and cortisol following envenoming. Scorpion envenoming causes an autonomic storm resulting in a massive release of catecholamines, angiotensin II, glucagon, and cortisol accompanied by changes in insulin secretion. The rise in the counter-regulatory hormones (glucagon, cortisol, and catecholamines) oppose the anabolic actions of insulin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion envenoming; Insulin; Glucagon; Cortisol; Mesobuthus tamulus concanesis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301999000100004

Purification and biological effects of a C-type lectin isolated from Bothrops moojeni
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Barbosa,PSF; Martins,AMC; Toyama,MH; Joazeiro,PP; Beriam,LOS; Fonteles,MC; Monteiro,HSA.
Snake venom proteins from the C-type lectin family have very distinct biological activities despite their highly conserved primary structure, which is homologous to the carbohydrate recognition region of true C-type lectins. We purified a lectin-like protein (BmLec) from Bothrops moojeni venom and investigated its effect on platelet aggregation, insulin secretion, antibacterial activity, and isolated kidney cells. The BmLec was purified using two chromatographic steps: affinity chromatography and reverse phase high performance liquid chromatography (HPLC). BmLec showed a dose-dependent platelet aggregation and significantly decreased the bacterial growth rate in approximately 15%. During scanning electron microscopy, the profile of Xanthomonas axonopodis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops moojeni; Kidney; Platelet aggregation; Insulin; Antibacterial activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300016

The use of insulin to improve fertility of timed-inseminated postpartum suckled beef cows
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Schneider,Augusto; Pfeifer,Luiz Francisco Machado; Schmitt,Eduardo; Bianchi,Ivan; Vieira,Marcelo Brandi; Xavier,Eduardo Gularte; Del Pino,Francisco Augusto Burkert; Corrêa,Marcio Nunes.
The objective of this work was to evaluate the effect of insulin alone or in association with equine chorionic gonadotropin (eCG) on the fertility of postpartum beef cows subjected to synchronization. A total of 340 cows was subjected to fixed time artificial insemination. In the trial 1, the cows were subjected to temporary weaning (TW), while in the trial 2 the same protocol was tested without TW. The addition of an insulin injection to a progesterone/eCG-based protocol without TW increased the pregnancy rate of beef cows with body condition score (BCS) equal to or lower than 2.5. Insulin had no effect on cows submitted to TW or with BCS equal to or higher than 3.0.
Palavras-chave: Bos taurus; ECG; Insulin; FTAI; Weaning.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001000023

Apparent cross-talk of two signaling pathways that regulate Zea mays coleoptile growth
Provedor de dados: Phyton: Revista Internacional de Botánica Experimental
Autores: Buentello Volante,B; Díaz de León-Sánchez,F; Rivera-Cabrera,F; Aguilar Caballero,R; Ponce-Valadez,M; Sánchez de Jiménez,E; Pérez-Flores,LJ.
Auxin and insulin promote Zea mays embryo growth, induce S6 ribosomal protein (S6rp) phosphorylation, and promote specific protein synthesis. The objective of this research was to test a possible cross-talk between insulin and auxin transduction pathways in Z. mays coleoptiles, typical auxin target tissues. Auxin and insulin produced differential quantitative and qualitative stimulation of cytoplasmic and ribosomal protein phosphorylation, and specific patterns of de novo synthesized cytoplasmic proteins. In addition, insulin induced S6rp phosphorylation was strongly inhibited by rapamycin, indicating target of rapamycin (TOR) kinase participation; auxin-induced S6rp phosphorylation was insensitive to this inhibitor. Phosphatidic acid (PA), a second...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Auxins; Insulin; Phosphatidic acid; ZmS6K; TOR; Plant growth.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000100015

Insulin Promotes the Expression of the Gluconeogenic Rate-Limiting Enzymes Phosphoenolpyruvate Carboxykinase (Pepck) and Glucose 6-Phosphatase (G6pase) through PI3k/Akt/mTOR Signaling Pathway in Goose Hepatocytes
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Xiong,XP; Song,Q; Han,CC; Gan,W; He,F; Wei,SH; Liu,HH; Xu,HY.
ABSTRACT To identify what makes insulin have an activating or inhibiting role in gluconeogenesis in goose hepatocytes and whether insulin regulates PEPCK and G6Pase through the PI3k/Akt/mTOR pathway or not, goose primary hepatocytes were isolated and cultured in vitro. After 12h cultured in serum-free medium, hepatocytes were incubated for 24 h in the medium with no addition (control) or with the addition of50, 100, and 150 nM of insulin, 1000 nM NVP-BEZ235, or co-addition of 150nM insulin and 1000nM NVP-BEZ235. Glucose concentration and PEPCK and G6Pase expression were determined. The results showed that PEPCK and G6Pase mRNA levels and activities were up regulated in the 50, 100, and 150nM insulin treatments, while glucose concentration was not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: G6Pase; Insulin; PEPCK; PI3k/Akt/mTOR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000300395

Linking dietary energy and skeletal development in the horse
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Staniar,William Burton.
Athletic production is what is sought from the horse. As mammary development is important to the dairy cow, skeletal development is important to horses meeting their production goals. As any integrative physiologist will appreciate, the variables that come together to result in optimal skeletal development are complex. Nutrition is one of these, and it contains two broad variables; the supply of dietary nutrients and energy. This presentation will focus on dietary energy and its links with skeletal development. I propose that it is not simply the amount of dietary energy, but the way and from that that energy is supplied that impacts skeletal development. Through an understanding of how dietary energy impact skeletal development, more precise feeding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cartilage; Equine; Glucose; Growth; Insulin; Somatotropic axis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300016

Oocyte production in Nellore cows supplemented with long-chain fatty acid soaps
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Souza,Moacir Rogério de; Santos,Geraldo Tadeu dos; Lima,Luciano Soares de; Mazucheli,Josmar; Zeoula,Lúcia Maria; Rigolon,Luiz Paulo; Cavalieri,Fábio Luiz Bim.
ABSTRACT This study was conducted to evaluate the effects of long-chain fatty acid soaps (LCFAS) of soybean oil on the production and quality of aspirated cumulus-oocyte complexes (COC) of lactating Nellore (Bos taurus indicus) cows. The effects of LCFAS on the plasma concentration of lipoproteins, total cholesterol, triacylglycerol, urea N, and insulin were also addressed. Thirty lactating Nellore cows were randomly assigned to one of two dietary groups. Cows were grazed on a Tifton 85 pasture throughout the experiment (100 days). Treatments consisted of a supplement with no fat included (CONT); and a supplement containing 47.2 g/kg of LCFAS on a dry matter basis. After 14 days of treatment, animals were subjected to 4.93±1.55 rounds of consecutive ovum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Insulin; Fat supplementation; Lipid profile; Ovum pick up.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000300138

Effect of different levels of total dietary fiber on the performance of sows in gestation and lactation
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Oelke,Carlos Alexandre; Ribeiro,Andréa Machado Leal; Noro,Mirela; Bernardi,Mari Lourdes; Denardin,Cristiane Casagrande; Nunes,Paula Raymundo; Veit,Fernando Cézar; Winckler,João Carlos.
ABSTRACT The present study evaluated the effect of variation in the amount of total dietary fiber (TDF) in sow diets, provided from 74 to 114 days of gestation, on the digestibility of nutrients and energy during gestation, as well as productivity and metabolism during gestation and lactation. Thirty-three sows were used in a randomized experimental design. The amounts of TDF in three treatment diets were 156, 223, and 282 g kg−1. The daily intake of nutrients and energy was similar for all diets the entire period of gestation, differing only in the volume of ration consumed, which was 2.1, 2.2, and 2.4 kg day−1 (days 74 to 90) and 2.5, 2.6, and 2.8 kg day−1 (days 91 to 111) for the 156, 223, and 282 g kg−1 TDF diets, respectively. During gestation, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Digestibility; Insulin; Piglets.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100525

ค่าดัชนีน้ำตาลในข้าวกล้องและข้าวกล้องงอก
Provedor de dados: Thai Agricultural Research Repository
Autores: Pakinee Akkaravessapong; Sunanta Wongpiyachon; Kunya Cheaupan; Watcharee Sukviwat; Pranee Maneenin; Angsutorn Wasusun; Sriwatana Songchitsomboon.
Glycemic index (GI) is the indexing of the glycemic response of available carbohydrate from food. GI is measured by blood glucose levels over 2 hours after took food compared with same amount of carbohydrate. Depend on GI, Food can be classified into 3 types which are High- (GI≥70), medium- (GI= 56-69) and low-glycemic-index (GI≤55). The recent studies already shown that consumption of Low-glycemic-index can decrease risk of developing diabetes, cardiovascular disease, colon and breast cancer. In this study, Identification of GI in 3 varieties of pre-germinated brown rice and 1 variety of brown rice, namely: Khao Dawk Mali 105, RD6 and Sang Yod were carried out. To determine the effect of different foods on the blood glucose and Insulin, pre-germinated...
Tipo: PhysicalObject
Palavras-chave: Glycemic index; Insulin; Diabetes; Pre-germinated brown rice; Brown rice; ข้าวกล้อง; ข้าวกล้องงอก; โรคเบาหวาน; อินซูลิน; ค่าดัชนีน้ำตาล.
Ano: 2014
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5642

ดัชนีน้ำตาลในข้าวกล้องและข้าวกล้องงอกของข้าว 4 พันธุ์
Provedor de dados: Thai Agricultural Research Repository
Autores: Sunanta Wongpiyachon; Sriwatana Songchitsomboon; Kunya Cheaupan; Watcharee Sukviwat; Pranee Maneenin; Angsutorn Wasusun.
Glycemic index (GI) is the indexing of the glycemic response of available carbohydrate from food. GI is measured by blood glucose levels over 2 hours after taking food compared with same amount of carbohydrate. Depend on GI, Food can be classified into 3 types which are high (GI ≥ 70), medium (GI = 56 - 69) and low (GI ≤ 55). The recent studies already shown that regular consumption of low-glycemic index food have beneficial effect on blood glucose control and reduce risk factors to complication of type 2 diabetes. In this study, Identification of GI in 4 rice varieties; Khao Dawk Mali 105 (KDML105), Pathumthani 1 (PTT1) - low amylose rice, Khao Tah Haeng 17 (KTH 17)-intermediate amylose rice and Jek Chuey 1 (JC 1) - high amylose rice has been undertaken....
Tipo: Collection
Palavras-chave: Brown rice; Pre-germinated brown rice; Glycemic index; Insulin; Diabetes; ข้าวกล้อง; ข้าวกล้องงอก; ค่าดัชนีน้ำตาล; อินซูลิน; โรคเบาหวาน.
Ano: 2015
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5862

ค่าดัชนีน้ำตาลในข้าวกล้องและข้าวกล้องงอก
Provedor de dados: Thai Agricultural Research Repository
Autores: Pakinee Akkaravessapong; Sunanta Wongpiyachon; Kanya Cheaupun; Watcharee Sukviwat; Pranee Maneenin; Angsutorn Wasusun; Sriwatana Songchitsomboon.
Glycemic index (GI) is the indexing of the glycemic response of available carbohydrate from food. GI is measured by blood glucose levels over 2 hours after took food compared with same amount of carbohydrate. Depend on GI, Food can be classified into 3 types which are High- (GI≥70), medium- (GI= 56-69) and low-glycemic-index (GI≤55). The recent studies already shown that consumption of low-glycemic-index can decrease risk of developing diabetes, cardiovascular disease, colon and breast cancer. In this study, Identification of GI in 3 varieties of pre-germinated brown rice and 1 variety of brown rice, namely: Khao Dawk Mali 105, RD6 and Sang Yod were carried out. To determine the effect of different foods on the blood glucose and Insulin, pre-germinated...
Tipo: Collection
Palavras-chave: Glycemic Index; Insulin; Diabetes; Pre-germinated brown rice; Brown rice ค่าดัชนีน้ำตาล อินซูลิน โรคเบาหวาน ข้าวกล้องงอก ข้าวกล้อง.
Ano: 2014
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5889

Dietary vegetable oils inclusion on the performance, hormonal levels and hsp 70 gene expression in broilers under heat stress
Provedor de dados: Acta Scientiarum: Animal Sciences
Autores: Tari, Ali Rafiei; Sadeghi, Ali Asghar; Mousavi, Seyed Naser.
The aim of the present study was to compare the effects of unsaturated and saturated oils on the performance, hormonal levels and hsp gene expression in broiler chickens exposed to heat stress. 300 one-day male broiler chicks were assigned to 4 treatments (Diets containing palm, corn, linseed or olive oils) with 5 replicates. At day 28 of age, 2 chickens were removed from each replicate, then blood samples and liver tissue samples were collected for analyses. Feeding linseed and olive oil reduced feed conversion ratio compared to corn and palm oils. The lowest level of insulin was for chickens fed linseed oil and corn oil. The highest level of corticosterone was found in chickens fed palm oil and the lowest level was for those received linseed oil....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corticosterone; Insulin; Heat shock protein; Heat tolerance.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/45517

Immune response, blood parameters and growth performance in broiler fed reduced protein diet supplemented with β-hydroxy-β-methyl butyrate and conjugated linoleic acid
Provedor de dados: Acta Scientiarum: Animal Sciences
Autores: Saki, Ali Asghar; Khoramabadi, Vahid; Nourian, Ali Reza; Zamani, Pouya.
  In this experiment a total of 300 d-old broilers (Ross 308) were randomized across 20 floor pens and reared up to 42 d. Treatments includes: 1) A normal-protein diet (NPD) was formulated according to the Ross 308 recommendation (2014) by corn and soybean meal, 2) A reduced-protein diet (RPD) was prepared with dietary protein reduced by 12 g kg-1 (1.2%) relative to the NPD, 3) HMB+ RDP, 4) CLA+RDP and 5) CLA+ HMB+ RDP. The results showed that broilers fed HMB diet had higher Average Daily Gain (ADG) and Feed Conversion Rate (FCR) significantly improved by HMB during the grower or the whole period (p &lt; 0.05). Titer of serum antibodies of Newcastle disease was maximum in treatment supplemented with CLA+HMB compared to the control (RPD) in day 42. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematological parameter; Insulin; Blood cell; Low protein diet; Protein synthesis.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/38000

Endocrine cells and nerve ganglia of the small intestine of the Opossum Didelphis aurita Wied-Neuwied, 1826 (Mammalia: Didelphidae)
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Freitas-Ribeiro,Gláucia M.; Fonseca,Cláudio C.; Sartori,Sirlene S.R.; Loures-Ribeiro,Alan; Neves,Clóvis A.
The nervous and endocrine systems jointly control intestinal movements, secretions of their glands and also participate of the processes of nutrient digestion and absorption. Therefore, the central objective of this study was to verify the existence of a possible relationship between the number of nervous cells and ganglia of the submucosal and myenteric plexuses and the number of endocrine cells in the small intestine of adult D. aurita. The utilized staining techniques were Grimelius, modified Masson-Fontana, direct immunoperoxidase and H-E. Argyrophillic, argentaffin and insulin immunoreactive endocrine cells do not numerically vary between the initial, mid and final regions of the duodenum, jejunum and ileum (P&gt;0.05), except for argyrophillic cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Argyrophillic cells; Argentaffin cells; Endocrine cells; Ganglia; Insulin.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300017

Subclinical Diabetes
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: LIMA,LUÍS M.T.R.
ABSTRACT Type 2 diabetes mellitus (T2DM) is increasing in prevalence worldwide, and those non-diagnosed or misdiagnosed comprise a significant group compared to those diagnosed. Accumulated scientific evidence indicate that the current diagnostic markers (fasting glycemia, 2h glycemia after an oral glucose load and HbA1c) are indeed late diagnostic criteria when considering the incidence of diabetes-related complications and comorbidities, which are also at high risk in some groups among normoglycemic individuals. Additionally, the earlier identification of future risk of diabetes is desirable since it would allow better adherence to preventive actions such as lifestyle intervention, ultimately avoiding complications and minimizing the economic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Diagnosis; Insulin; Subclinical diabetes; Metabolic syndrome.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200591

Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets
Provedor de dados: Biological Research
Autores: CUNHA,DANIEL A; AMARAL,MARIA EC; CARVALHO,CAROLINA PF; COLLARES-BUZATO,CARLA B; CARNEIRO,EVERARDO M; BOSCHERO,ANTONIO C.
During pregnancy and the perinatal period of life, prolactin (PRL) and other lactogenic substances induce adaptation and maturation of the stimulus-secretion coupling system in pancreatic &amp;#946;-cells. Since the SNARE molecules, SNAP-25, syntaxin 1, VAMP-2, and synaptotagmins participate in insulin secretion, we investigated whether the improved secretory response to glucose during these periods involves alteration in the expression of these proteins. mRNA was extracted from neonatal rat islets cultured for 5 days in the presence of PRL and from pregnant rats (17th-18th days of pregnancy) and reverse transcribed. The expression of genes was analyzed by semi-quantitative RT-PCR assay. The expression of proteins was analyzed by Western blotting and...
Tipo: Journal article
Palavras-chave: Insulin; Pancreatic islets; Pregnant rats; Prolactin; SNARE proteins; Synaptotagmin.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300016

Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells
Provedor de dados: Biological Research
Autores: JAIMOVICH,ENRIQUE; ESPINOSA,ALEJANDRA.
We studied the effect of IGF-I, insulin and testosterone on intracellular Ca2+ in cultured muscle cells. Insulin produced a fast (&lt;1 s) and transient [Ca2+] increase lasting less than 10 s. IGF-I induced a transient [Ca2+] increase, reaching a fluorescence peak 6 s after stimulus, to return to basal values after 60 s. Testosterone induced delayed (35 s) and long lasting (100-200 s) signals, frequently associated with oscillations. IGF-I, testosterone and electrical stimulation-induced Ca2+ signals were shown to be dependent on IP3 production. All of these Ca2+ signals were blocked by inhibitors of the IP3 pathway. On the other hand, insulin-induced Ca2+ increase was dependent on ryanodine receptors and blocked by either nifedipine or ryanodine. The...
Tipo: Journal article
Palavras-chave: Skeletal; Transcription factors; Insulin; IGF-I; Testosterone.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400018

Properties of voltage-gated Ca2+ currents measured from mouse pancreatic &amp;#946;-cells in situ
Provedor de dados: Biological Research
Autores: MEARS,DAVID; ROJAS,EDUARDO.
We used the single-microelectrode voltage-clamp technique to record ionic currents from pancreatic &amp;#946;-cells within intact mouse islets of Langerhans at 37C, the typical preparation for studies of glucose-induced "bursting" electrical activity. Cells were impaled with intracellular microelectrodes, and voltage pulses were applied in the presence of tetraethylammonium. Under these conditions, a voltage-dependent Ca2+ current (I Cav), containing L-type and non-L-type components, was observed. The current measured in situ was larger than that measured in single cells with whole-cell patch clamping, particularly at membrane potentials corresponding to the action potentials of &amp;#946;-cell electrical activity. The temperature dependence of I Cav was...
Tipo: Journal article
Palavras-chave: Islet of Langerhans; Insulin; Stimulus-secretion coupling; Ion channel; Inactivation kinetics; BAPTA.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300012

Insulin or insulin-like studies on unicellular organisms: a review
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Souza,Alzira Martins Ferreira de; López,Jorge A.
This paper presents a review of studies about insulin and insulin-like substances in prokaryotes, eukaryotes and fungi have been published over the last two decades, constituting an updating of our previous review (1988) which included references to invertebrate insulin or insulin-like substances both in uni- and pluricellular Monera, Protoctista, Fungii and Animal species. This present article reviews experiments and evidence obtained using modern techniques in the understanding of molecule evolution and behaviour, which confirm its very ancient molecular structure origin. The involvement of insulin-like and related material in signalling biological pathway and modulator effects is also reported.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Unicellular; Invertebrate; Insulin; Insulin-like; Insulin receptor.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600017

Biological response of spontaneously hypertensive rats to the streptozotocin administration
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Costa,Virginia Alice Vieira da; Vianna,Lucia Marques.
The sensitivity of adult spontaneously hypertensive rats (SHR) to the diabetogenic effect of streptozotocin (STZ) was studied. The animals were subdivided into three groups: control (citrate buffer), streptozotocin 40 mg/kg or 50 mg/kg, and general biologic parameters were analyzed, in addition to systolic blood pressure, blood glucose and insulin levels determinations. Both doses were able to induce hyperglycemia above 300 mg/dl; however, 50 mg/kg provoked a more pronounced physiological alterations in body weight, diuresis, water and food intake. There was no change on systolic blood pressure with either dose. Results suggested that SHRs did not need doses of streptozotocin above 40mg/kg in order to produce diabetes probably because this strain was much...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptozotocin; SHR; Diabetes; Blood pressure; Insulin.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100006

Association of postalimentary lipemia with atherosclerotic manifestations
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Tentor,J.; Nakamura,R.T.; Gidlund,M.; Barros-Mazon,S.; Harada,L.M.; Zago,V.S.; Oba,J.F.; Faria,E.C. de.
We identified different lipemic and metabolic responses after the ingestion of a standardized meal by healthy adults and related them to atherosclerotic markers. Samples from 60 normolipidemic adults were collected before and after a liquid meal (40 g fat/m² body surface) at 0, 2, 4, 6, and 8 h for measurements of lipids, free fatty acids (FFA), insulin, cholesteryl ester transfer protein (CETP), autoantibodies to epitopes of oxidized LDL (oxLDL Ab), lipolytic activities, and apolipoprotein E polymorphism. Mean carotid intima-media thickness (cIMT) was determined by Doppler ultrasound. The volunteers were classified into early (N = 39) and late (N = 31) triacylglycerol (TAG) responders to the test meal. Late responders showed lower HDL cholesterol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Postalimentary lipemia; Autoantibodies to epitopes of oxidized LDL; Carotid intima-media thickness; Insulin; Free fatty acids.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100014

Histologic distribution of insulin and glucagon receptors
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Watanabe,M.; Hayasaki,H.; Tamayama,T.; Shimada,M.
Insulin and glucagon are the hormonal polypeptides secreted by the B and A cells of the endocrine pancreas, respectively. Their major physiologic effects are regulation of carbohydrate metabolism, but they have opposite effects. Insulin and glucagon have various physiologic roles, in addition to the regulation of carbohydrate metabolism. The physiologic effects of insulin and glucagon on the cell are initiated by the binding of each hormone to receptors on the target cells. Morphologic studies may be useful for relating biochemical, physiologic, and pharmacologic information on the receptors to an anatomic background. Receptor radioautography techniques using radioligands to label specific insulin and glucagon receptors have been successfully applied to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radioautography; Insulin; Glucagon; Receptor; Distribution.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200008

Effect of Camellia sinensis teas on left ventricular hypertrophy and insulin resistance in dyslipidemic mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ferreira,M.C.L.; Lima,L.N.; Cota,L.H.T.; Costa,M.B.; Orsi,P.M.E.; Espíndola,R.P.; Albanez,A.V.; Rosa,B.B.; Carvalho,M.G.S.; Garcia,J.A.D.
The control of dyslipidemia using plants is an important subject of studies since it has numerous benefits in cardiovascular protection. The objective of this study was to evaluate the effect of three Camellia sinensis L. teas (green, red, and white) on left ventricular hypertrophy and insulin resistance in low-density lipoprotein receptor knockout (LDLr-/-) mice fed a high-fat diet. The LDLr-/- mice were divided into four experimental groups: Group C: standard feed; Group CT: standard feed and three teas, Group HL: high-fat feed; HLT Group: high-fat feed and three teas. The three types of tea (green, red, and white) originated from different processing of the Camellia sinensis L. plant, and were administered associated once a day at a dose of 25 mg/kg by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipids; Cardiovascular system; Tea; Glucose; Insulin.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500607

Viscosity of gums in vitro and their ability to reduce postprandial hyperglycemia in normal subjects
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Brenelli,S.L.; Campos,S.D.S.; Saad,M.J.A.
Experiments were carried out in vitro with three viscous polysaccharides (guar gum, pectin, and carboxymethylcellulose (CMC)) of similar initial viscosity submitted to conditions that mimic events occurring in the stomach and duodenum, and their viscosity in these situations was compared to their actions on postprandial hyperglycemia in normal human subjects. Guar gum showed greater viscosity than the other gums during acidification and/or alkalinization and also showed larger effects on plasma glucose levels (35% reduction in maximum rise in plasma glucose) and on the total area under the curve of plasma glucose (control: 20,314 ± 1007 mg dl-1 180 min-1 vs guar gum: 18,277 ± 699 mg dl-1 180 min-1, P&amp;lt;0.01). Pectin, which showed a marked reduction in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Viscosity of dietary fiber; Glucose tolerance test; Insulin; Guar gum; Carboxymethylcellulose; Pectin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200009

Neutrophil function and metabolism in individuals with diabetes mellitus
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Alba-Loureiro,T.C.; Munhoz,C.D.; Martins,J.O.; Cerchiaro,G.A.; Scavone,C.; Curi,R.; Sannomiya,P.
Neutrophils act as first-line-of-defense cells and the reduction of their functional activity contributes to the high susceptibilityto and severity of infections in diabetes mellitus. Clinical investigations in diabetic patients and experimental studies in diabetic rats and mice clearly demonstrated consistent defects of neutrophil chemotactic, phagocytic and microbicidal activities. Other alterations that have been reported to occur during inflammation in diabetes mellitus include: decreased microvascular responses to inflammatory mediators such as histamine and bradykinin, reduced protein leakage and edema formation, reduced mast cell degranulation, impairment of neutrophil adhesionto the endothelium and migration to the site of inflammation, production...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutrophils; Inflammation; Diabetes mellitus; Insulin; Metabolism and cellular function.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800003

Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Follicle-stimulating hormone (FSH) and insulin regulate glycide metabolism in Sertoli cells, thus stimulating lactate production. These stimulatory effects of FSH and insulin do not require protein synthesis, suggesting a modulation of enzyme activity and/or regulation of glucose transport. The present investigation was performed to characterize the hormonal control of lipid metabolism in Sertoli cells. The data indicate that FSH and insulin have a regulatory effect on lipid metabolism in Sertoli cells. After 8 h of preincubation with insulin (5 µg/ml), the activity of the enzyme ATP-citrate lyase in cultured Sertoli cells was increased from 0.19 to 0.34 nmol NAD+ formed µg protein-1 min-1. FSH (100 ng/ml) had no effect on this enzyme. Glycerol phosphate...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sertoli cells; Lipids; FSH; Insulin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500004

Modulation of hormone secretion by functional electrical stimulation of the intact and incompletely dysfunctional dog pancreas
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Rozman,J.; Bunc,M.; Zorko,B.
The purpose of the present study was to modulate the secretion of insulin and glucagon in Beagle dogs by stimulation of nerves innervating the intact and partly dysfunctional pancreas. Three 33-electrode spiral cuffs were implanted on the vagus, splanchnic and pancreatic nerves in each of two animals. Partial dysfunction of the pancreas was induced with alloxan. The nerves were stimulated using rectangular, charge-balanced, biphasic, and constant current pulses (200 µs, 1 mA, 20 Hz, with a 100-µs delay between biphasic phases). Blood samples from the femoral artery were drawn before the experiment, at the beginning of stimulation, after 5 min of stimulation, and 5 min after the end of stimulation. Radioimmunoassay data showed that in the intact pancreas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional electrical stimulation; Implanted electrodes; Autonomic nerves; Pancreatic secretion; Insulin; Glucagon.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300012

Diet plus insulin compared to diet alone in the treatment of gestational diabetes mellitus: a systematic review
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Giuffrida,F.M.A.; Castro,A.A.; Atallah,A.N.; Dib,S.A.
Fetuses of mothers with gestational diabetes mellitus are at increased risk to develop perinatal complications mainly due to macrosomia. However, in view of the marked heterogeneity of this disease, it seems difficult to set guidelines for diagnosis and treatment. This complicates the choice of assigning patients either to diet or to insulin therapy. Also of concern is how much benefit could be expected from insulin therapy in preventing fetal complications in these patients. In a systematic review of the literature assessing the efficacy of insulin in preventing macrosomia in fetuses of mothers with gestational diabetes, we found six randomized controlled trials comparing diet alone to diet plus insulin. The studies included a total of 1281 patients (644...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diabetes; Gestational; Diet therapy; Fetal macrosomia; Insulin; Meta-analysis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000004

Purification of bovine pancreatic glucagon as a by-product of insulin production
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Andrade,A.S.R.; Vilela,L.; Tunes,H.
A process for purifying bovine pancreatic glucagon as a by-product of insulin production is described. The glucagon-containing supernatant from the alkaline crystallization of insulin was precipitated using ammonium sulfate and isoelectric precipitation. The isoelectric precipitate containing glucagon was then purified by ion-exchange chromatography on Q-Sepharose FF, gel filtration on Sephadex G-25 and ion-exchange chromatography on S-Sepharose FF. A pilot scale test was performed with a recovery of 87.6% and a purification factor of 8.78 for the first chromatographic step, a recovery of 75.1% and a purification factor of 3.90 for the second, and a recovery of 76.2% and a purification factor of 2.36 for the last one. The overall yield was 50%, a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucagon; Protein purification; Gel filtration; Ion-exchange chromatography; Insulin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200006

A protein with amino acid sequence homology to bovine insulin is present in the legume Vigna unguiculata (cowpea)
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Venâncio,T.M.; Oliveira,A.E.A.; Silva,L.B.; Machado,O.L.T.; Fernandes,K.V.S.; Xavier-Filho,J.
Since the discovery of bovine insulin in plants, much effort has been devoted to the characterization of these proteins and elucidation of their functions. We report here the isolation of a protein with similar molecular mass and same amino acid sequence to bovine insulin from developing fruits of cowpea (Vigna unguiculata) genotype Epace 10. Insulin was measured by ELISA using an anti-human insulin antibody and was detected both in empty pods and seed coats but not in the embryo. The highest concentrations (about 0.5 ng/µg of protein) of the protein were detected in seed coats at 16 and 18 days after pollination, and the values were 1.6 to 4.0 times higher than those found for isolated pods tested on any day. N-terminal amino acid sequencing of insulin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Insulin-like; Cowpea; Vigna unguiculata; Developing fruits; Sequence homology; Evolution.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900004

Leptin is not associated independently with hypertension in Japanese-Brazilian women
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Almeida-Pititto,B.; Gimeno,S.G.A.; Freire,R.D.; Ribeiro-Filho,F.F.; Ferreira,S.R.G.
We evaluated the relationship of leptin with hypertension adjusted for body mass index (BMI) and/or waist circumference in a population of Japanese-Brazilian women aged  &gt; or = 30 years with centrally distributed adiposity. After excluding diabetic subjects, the study subjects - who participated in a population-based study on the prevalence of metabolic syndrome - showed prevalence rates of obesity (BMI  &gt; or = 25 kg/m²) and central adiposity (waist  &gt; or = 80 cm) of 32.0 and 37.8%, respectively. The hypertensive group (N = 162) was older, had higher BMI (24.9 ± 4.2 vs 23.3 ± 3.4 kg/m², P &lt; 0.001), waist circumference (81.1 ± 10.1 vs 76.3 ± 8.2 cm, P &lt; 0.001) and insulin levels (8.0 ± 6.2 vs 7.1 ± 4.9 µU/mL, P &lt; 0.05) than the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Body fat distribution; Insulin; Leptin; Centrally obese; Metabolic syndrome.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100012

Time course of changes in heart rate and blood pressure variability in streptozotocin-induced diabetic rats treated with insulin
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Schaan,B.D.; Maeda,C.Y.; Timm,H.B.; Medeiros,S.; Moraes,R.S.; Ferlin,E.; Fernandes,T.G.; Ribeiro,J.P.; Schmid,H.; Irigoyen,M.C.
Autonomic neuropathy is a frequent complication of diabetes associated with higher morbidity and mortality in symptomatic patients, possibly because it affects autonomic regulation of the sinus node, reducing heart rate (HR) variability which predisposes to fatal arrhythmias. We evaluated the time course of arterial pressure and HR and indirectly of autonomic function (by evaluation of mean arterial pressure (MAP) variability) in rats (164.5 ± 1.7 g) 7, 14, 30 and 120 days after streptozotocin (STZ) injection, treated with insulin, using measurements of arterial pressure, HR and MAP variability. HR variability was evaluated by the standard deviation of RR intervals (SDNN) and root mean square of successive difference of RR intervals (RMSSD). MAP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Streptozotocin; Insulin; Autonomic nervous system.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900006

Insulin impairs the maturation of chondrocytes in vitro
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Torres,E.S.; Andrade,C.V.; Fonseca,E.C.; Mello,M.A.; Duarte,M.E.L.
The precise nature of hormones and growth factors directly responsible for cartilage maturation is still largely unclear. Since longitudinal bone growth occurs through endochondral bone formation, excess or deficiency of most hormones and growth factors strongly influences final adult height. The structure and composition of the cartilaginous extracellular matrix have a critical role in regulating the behavior of growth plate chondrocytes. Therefore, the maintenance of the three-dimensional cell-matrix interaction is necessary to study the influence of individual signaling molecules on chondrogenesis, cartilage maturation and calcification. To investigate the effects of insulin on both proliferation and induction of hypertrophy in chondrocytes in vitro we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chondrocytes; Insulin; Micromass cultures; Apoptosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900007

Consequences of cerebroventricular insulin injection on renal sodium handling in rats: effect of inhibition of central nitric oxide synthase
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Oliveira,P.C.; Michelotto,J.B.; Zapparoli,A.; Boer,P.A.; Gontijo,J.A.R.
In the present study, we investigated the effects of acute intracerebroventricular (icv) insulin administration on central mechanisms regulating urinary sodium excretion in simultaneously centrally NG-nitro-L-arginine methylester (L-NAME)-injected unanesthetized rats. Male Wistar-Hannover rats were randomly assigned to one of five groups: a) icv 0.15 M NaCl-injected rats (control, N = 10), b) icv dose-response (1.26, 12.6 and 126 ng/3 µL) insulin-injected rats (N = 10), c) rats icv injected with 60 µg L-NAME in combination with NaCl (N = 10) or d) with insulin (N = 10), and e) subcutaneously insulin-injected rats (N = 5). Centrally administered insulin produced an increase in urinary output of sodium (NaCl: 855.6 ± 85.1 Δ%/min; 126 ng insulin: 2055 ± 310.6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central nervous system; Intracerebroventricular; Nitric oxide inhibition; Insulin; Natriuresis; Lithium clearance.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200013

Biphasic modulation of insulin receptor substrate-1 during goitrogenesis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Grozovsky,R.; Morales,M.M.; Carvalho,D.P.
Insulin receptor substrate-1 (IRS-1) is the main intracellular substrate for both insulin and insulin-like growth factor I (IGF-I) receptors and is critical for cell mitogenesis. Thyrotropin is able to induce thyroid cell proliferation through the cyclic AMP intracellular cascade; however, the presence of either insulin or IGF-I is required for the mitogenic effect of thyroid-stimulating hormone (TSH) to occur. The aim of the present study was to determine whether thyroid IRS-1 content is modulated by TSH in vivo. Strikingly, hypothyroid goitrous rats, which have chronically high serum TSH levels (control, C = 2.31 ± 0.28; methimazole (MMI) 21d = 51.02 ± 6.02 ng/mL, N = 12 rats), when treated with 0.03% MMI in drinking water for 21 days, showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid; Goiter; Insulin receptor substrate; Insulin; Thyrotropin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500011

The leaves of green plants as well as a cyanobacterium, a red alga, and fungi contain insulin-like antigens
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Silva,L.B.; Santos,S.S.S.; Azevedo,C.R.; Cruz,M.A.L.; Venâncio,T.M.; Cavalcante,C.P.; Uchôa,A.F.; Astolfi Filho,S.; Oliveira,A.E.A.; Fernandes,K.V.S.; Xavier-Filho,J.
We report the detection of insulin-like antigens in a large range of species utilizing a modified ELISA plate assay and Western blotting. We tested the leaves or aerial parts of species of Rhodophyta (red alga), Bryophyta (mosses), Psilophyta (whisk ferns), Lycopodophyta (club mosses), Sphenopsida (horsetails), gymnosperms, and angiosperms, including monocots and dicots. We also studied species of fungi and a cyanobacterium, Spirulina maxima. The wide distribution of insulin-like antigens, which in some cases present the same electrophoretic mobility as bovine insulin, together with results recently published by us on the amino acid sequence of an insulin isolated from the seed coat of jack bean (Canavalia ensiformis) and from the developing fruits of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Insulin-like; Antigens; Evolution; Plants; Cyanobacteria; Algae; Fungi.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300004

Specific insulin and proinsulin in normal glucose tolerant first-degree relatives of NIDDM patients
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Coifman,R.; Dalbosco,I.S.; Russo,E.M.K.; Moisés,R.S.
In order to identify early abnormalities in non-insulin-dependent diabetes mellitus (NIDDM) we determined insulin (using an assay that does not cross-react with proinsulin) and proinsulin concentrations. The proinsulin/insulin ratio was used as an indicator of abnormal ß-cell function. The ratio of the first 30-min increase in insulin to glucose concentrations following the oral glucose tolerance test (OGTT; I30-0/G30-0) was taken as an indicator of insulin secretion. Insulin resistance (R) was evaluated by the homeostasis model assessment (HOMA) method. True insulin and proinsulin were measured during a 75-g OGTT in 35 individuals: 20 with normal glucose tolerance (NGT) and without diabetes among their first-degree relatives (FDR) served as controls, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; NIDDM; Proinsulin; First-degree relatives.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100010

Effect of high-fat diets on body composition, lipid metabolism and insulin sensitivity, and the role of exercise on these parameters
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Coelho,D.F.; Pereira-Lancha,L.O.; Chaves,D.S.; Diwan,D.; Ferraz,R.; Campos-Ferraz,P.L.; Poortmans,J.R.; Lancha Junior,A.H.
Dietary fat composition can interfere in the development of obesity due to the specific roles of some fatty acids that have different metabolic activities, which can alter both fat oxidation and deposition rates, resulting in changes in body weight and/or composition. High-fat diets in general are associated with hyperphagia, but the type of dietary fat seems to be more important since saturated fats are linked to a positive fat balance and omental adipose tissue accumulation when compared to other types of fat, while polyunsaturated fats, omega-3 and omega-6, seem to increase energy expenditure and decrease energy intake by specific mechanisms involving hormone-sensitive lipase, activation of peroxisome proliferator-activated receptor α (PPARα) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fat; Obesity; Insulin; PUFA; Diet; Exercise.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000001

Positive correlation of serum leptin with estradiol levels in patients with polycystic ovary syndrome
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Mendonça,H.C.; Montenegro Junior,R.M.; Foss,M.C.; Silva de Sá,M.F.; Ferriani,R.A.
Patients with polycystic ovary syndrome (PCOS) usually are obese, insulin resistant and hyperinsulinemic. The known association between leptin, obesity andinsulin action suggests that leptin may have a role in PCOS but this has only been addressed peripherally. This study was designed to assess the relationship between serum leptin and the anthropometric, metabolic and endocrine variables of obese (body mass index, BMI ³30 kg/m²) and non-obese (BMI &lt;30 kg/m²) PCOS patients. Twenty-eight PCOS patients and 24 control women subdivided into obese and non-obese groups were evaluated. Leptin, androgens, lipids, gonadotrophins and insulin-glucose response to the oral glucose tolerance test were measured by radioimmunoassay in all participants. The assays were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptin; Polycystic ovary syndrome; Insulin; Insulin sensitivity; Estrogen.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500015

Low levels of sex hormone-binding globulin and hyperproinsulinemia as markers of increased pancreatic ß-cell demand in men
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Reis,A.F.; Miranda,W.L.; Chacra,A.R.
Low levels of sex hormone-binding globulin (SHBG) are considered to be an indirect index of hyperinsulinemia, predicting the later onset of diabetes mellitus type 2. In the insulin resistance state and in the presence of an increased pancreatic ß-cell demand (e.g. obesity) both absolute and relative increases in proinsulin secretion occur. In the present study we investigated the correlation between SHBG and pancreatic ß-cell secretion in men with different body compositions. Eighteen young men (30.0 ± 2.4 years) with normal glucose tolerance and body mass indexes (BMI) ranging from 22.6 to 43.2 kg/m2 were submitted to an oral glucose tolerance test (75 g) and baseline and 120-min blood samples were used to determine insulin, proinsulin and C-peptide by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Proinsulin; Sex hormone-binding globulin; Obesity.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200006

Effect of an aqueous extract of Scoparia dulcis on blood glucose, plasma insulin and some polyol pathway enzymes in experimental rat diabetes
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Latha,M.; Pari,L.
The effects of an aqueous extract of the plant Scoparia dulcis (200 mg/kg) on the polyol pathway and lipid peroxidation were examined in the liver of streptozotocin adult diabetic male albino Wistar rats. The diabetic control rats (N = 6) presented a significant increase in blood glucose, sorbitol dehydrogenase, glycosylated hemoglobin and lipid peroxidation markers such as thiobarbituric acid reactive substances (TBARS) and hydroperoxides, and a significant decrease in plasma insulin and antioxidant enzymes such as glutathione peroxidase (GPx), glutathione-S-transferase (GST) and reduced glutathione (GSH) compared to normal rats (N = 6). Scoparia dulcis plant extract (SPEt, 200 mg kg-1 day-1) and glibenclamide (600 µg kg-1 day-1), a reference drug, were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione peroxidase; Glutathione-S-transferase; Insulin; Lipid peroxidation; Scoparia dulcis; Sorbitol dehydrogenase; Diabetes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400015

The hepatic ectonucleotide pyrophosphatase/phosphodiesterase 1 gene mRNA abundance is reduced by insulin and induced by dexamethasone
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ma,Huiwen; Wang,Ping; Jin,Dan; Jia,Ting; Mao,Hong; Zhang,Jiandi; Zhao,Shi.
Hormones regulate hepatic gene expressions to maintain metabolic homeostasis. Ectonucleotide pyrophosphatase/phosphodiesterase 1 has been thought to interfere with insulin signaling. To determine its potential role in the regulation of metabolism, we analyzed its gene (Enpp1) expression in the liver of rats experiencing fasting and refeeding cycles, and in primary rat hepatocytes and human hepatoma HepG2 cells treated with insulin and dexamethasone using northern blot and real-time PCR techniques. Hepatic Enpp1 expression was induced by fasting and reduced by refeeding in the rat liver. In primary rat hepatocytes and HepG2 hepatoma cells, insulin reduced Enpp1 mRNA abundance, whereas dexamethasone induced it. Dexamethasone disrupted the insulin-reduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ectonucleotide pyrophosphatase/phosphodiesterase 1; Insulin; Dexamethasone; Hepatocytes; Regulation of gene expression.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400609

Malnutrition during lactation as a metabolic imprinting factor inducing the feeding pattern of offspring rats when adults: The role of insulin and leptin
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Moura,A.S.; Franco de Sá,C.C.N.; Cruz,H.G.; Costa,C.L.
The aim of the present study was to determine the impact of malnutrition during early postnatal life and the feeding pattern of rat offspring when adults (2 months and 1 year old). In comparison with rats normally fed during lactation, we observed that adult offspring displayed a faster process of feeding reduction when a protein-free diet was offered. In addition, we studied the concentration of insulin and leptin in the lactating pups (10 days) and when these offspring became adult after the onset of a new feeding pattern induced by the protein-free diet. When the diet was changed at 60 days, the offspring malnourished during lactation displayed, after 3 days, a food intake reduction around 41.4 vs 14.2% of the control group. At 10 days of life, plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nutrition; Growth; Insulin; Leptin.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500016

Effect of intracerebroventricularly injected insulin on urinary sodium excretion by cerebroventricular streptozotocin-treated rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Macedo,R.F.; Furlan,F.C.; Marshall,P.S.; Michelotto,J.B.; Gontijo,J.A.R.
Recent evidence suggests that insulin may influence many brain functions. It is known that intracerebroventricular (icv) injection of nondiabetogenic doses of streptozotocin (STZ) can damage insulin receptor signal transduction. In the present study, we examined the functional damage to the brain insulin receptors on central mechanisms regulating glomerular filtration rate and urinary sodium excretion, over four periods of 30 min, in response to 3 µl insulin or 0.15 NaCl (vehicle) injected icv in STZ-treated freely moving Wistar-Hannover rats (250-300 g). The icv cannula site was visually confirmed by 2% Evans blue infusion. Centrally administered insulin (42.0 ng/µl) increased the urinary output of sodium (from 855.6 ± 85.1 to 2055 ± 310.6 delta%/min; N =...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Central nervous system; Insulin; Natriuresis; Streptozotocin; Lithium clearance.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900008

Specific insulin and proinsulin secretion in glucokinase-deficient individuals
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pardini,V.C.; Velho,G.; Reis,R.; Purisch,S.; Blanché,H.; Vieira,J.G.H.; Moisés,R.C.S.
Glucokinase (GCK) is an enzyme that regulates insulin secretion, keeping glucose levels within a narrow range. Mutations in the glucokinase gene cause a rare form of diabetes called maturity-onset diabetes of the young (MODY). An early onset (less than 25 years), autosomal dominant inheritance and low insulin secretion stimulated by glucose characterize MODY patients. Specific insulin and proinsulin were measured in serum by immunofluorimetric assays (IFMA) during a 75-g oral glucose tolerance test (OGTT). Two kindreds (SA and LZ) were studied and compared to non-diabetic unrelated individuals (control group 1) matched for age and body mass index (BMI). In one kindred, some of these subjects were also obese (BMI &amp;gt;26 kg/m2), and other family members...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proinsulin; Insulin; MODY; Glucokinase; Obesity.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400008

Metabolic dysregulation in early onset psychiatric disorder before and after exposure to antipsychotic drugs
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Shah,Rehmat; Subhan,Fazal; Sultan,Syed Muhammad; Haq,Matiul; Ahmad,Hussain; Khan,Qasim Riaz; Ali,Gowhar; Ullah,Sami; Ullah,Ihsan.
Antipsychotic Drugs (APDs) are being widely prescribed to treat various disorders, including schizophrenia and bipolar disorder; however, abnormal glucose metabolism and weight gain have been reported with Atypical Anti-Psychotic drugs (AAPDs) that can lead to insulin-resistance and type 2 diabetes mellitus. The study was designed to assess various biochemical parameters including insulin and blood sugar before and after exposure to APDs in order to exclude the involvement of psychiatric disorders and certain other factors in metabolic dysregulations. Fifty seven APDs-naïve patients with first episode psychosis were divided into six groups who received olanzapine, quetiapine, risperidone, aripiprazole, haloperidol or combination of olanzapine with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olanzapine; Quetiapine; Risperidone; Antipsychotics; Pancreas; Insulin.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100535

The role of selenium in insulin resistance
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Fontenelle,Larissa Cristina; Feitosa,Mayara Monte; Morais,Jennifer Beatriz Silva; Severo,Juliana Soares; Freitas,Taynáh Emannuelle Coelho de; Beserra,Jéssica Batista; Henriques,Gilberto Simeone; Marreiro,Dilina do Nascimento.
ABSTRACT In recent years, there has been growing interest in clarifying the pathogenesis of some chronic diseases, such as obesity and type 2 diabetes mellitus. Metabolic alterations in these diseases are characterized by chronic hyperglycemia and insulin resistance. Studies have demonstrated the participation of minerals in the pathogenesis of insulin resistance, more specifically their involvement in the synthesis and regulation of insulin. Selenium is an anti-inflammatory and antioxidant micronutrient that is essential for the activity of selenoproteins. Two selenoproteins (glutathione peroxidase and selenoprotein P) are known to be involved in the insulin signaling pathway. The aim of this review is to provide an update on the role of selenium in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Selenium/insulin resistance; Insulin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100401

Ethnopharmacological activity of Hedera nepalensis K. Koch extracts and lupeol against alloxan-induced type I diabetes
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Hashmi,Waleed Javed; Ismail,Hammad; Jafri,Laila; Mirza,Bushra.
In this study, we investigated the protective effects of Hedera nepalensis crude extract, its fractions and lupeol in alloxan-induced diabetic rats. Lupeol and n-hexane (HNN) fraction significantly reduced the blood glucose level by increasing insulin level in time dependent manner, and also significantly increased amylase and lipase activity in diabetic rats. Elevated levels of alanine transaminases (ALT), aspartate transaminases (AST), thiobarbituric acid reactive substances (TBARS), nitrite, hydrogen peroxide (H2O2), total bilirubin and total protein in blood serum were efficiently restored to normal levels. Suppressed enzymatic activity of catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and peroxidase (POD) were also restored to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Amylase; Diabetes mellitus; Insulin; Hedera nepalensis; Lupeol.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100597

Insulin-loaded polymeric mucoadhesive nanoparticles: development, characterization and cytotoxicity evaluation
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Gatti,Tiago Henrique Honorato; Eloy,Josimar Oliveira; Ferreira,Leonardo Miziara Barboza; Silva,Isabel Cristine da; Pavan,Fernando Rogério; Gremião,Maria Palmira Daflon; Chorilli,Marlus.
Abstract Mucoadhesive nanoparticles are particularly interesting for delivery through nasal or pulmonary routes, as an approach to overcome the mucociliary clearance. Moreover, these nanoparticles are attractive for peptide and protein delivery, particularly for insulin to treat diabetes, as an alternative to conventional parenteral administration. Thus, chitosan, a cationic mucoadhesive polysaccharide found in shells of crustaceans, and the negatively-charged dextran sulfate are able to form nanoparticles through ionic condensation, representing a potential insulin carrier. Herein, chitosan/dextran sulfate nanoparticles at various ratios were prepared for insulin loading. Formulations were characterized for particle size, zeta potential, encapsulation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Chitosan; Dextran-Sulfate; Nanoparticles; Mucoadhesion.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100623

Plant insulin or glucokinin: a conflicting issue
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Xavier-Filho,José; Oliveira,Antônia Elenir A.; Silva,Luciana Belarmindo da; Azevedo,Cassiana Rocha; Venâncio,Thiago Motta; Machado,Olga Lima T.; Oliva,Maria Luiza; Fernandes,Kátia Valevski S.; Xavier-Neto,José.
The presence of insulin in plants is not accepted by the scientific community in general. In this review we discuss this paradigm and retrieve information that strongly suggests that insulin is indeed found in plants. We present results, which indicate that a protein molecule with the same amino acid sequence as bovine insulin is expressed in leguminous plants. Additionally, we provide evidence that proteins associated with insulin signalling pathways in vertebrates are also found in association with insulin-like molecules in plants.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Glucokinin; Peptide hormone; Plants; Amino acid sequence; Evolution.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000200002

Milk production in Saanen goats treated with repeated low doses of intermediate-release insulin during early lactation
Provedor de dados: Ciência Rural
Autores: Menezes,Erika Bezerra de; Salles,Maria Gorete Flores; Silva,Cleidson Manoel Gomes da; Fernandes,César Carneiro Linhares; Galeati,Giovanna; Araújo,Airton Alencar de; Rondina,Davide.
ABSTRACT:  The effect of insulin administration on the productive responses of Saanen goats during early lactation was investigated. Ten of 20 adult females were subjected to subcutaneous administration of intermediate-acting insulin (0.14UI/kg body weight) at 2, 9, and 14 days postpartum. Milk yield was measured twice daily for 13 weeks and milk samples were collected to measure protein and fat contents. Plasma levels of progesterone, insulin, non-esterifies fatty acids, glucose and other metabolites were measured. Results showed a significantly increased effect of insulin treatment on the content of milk fat and protein; moreover, milk production in the first and second postpartum weeks were higher than control group. The peak of lactation in the insulin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goat; Insulin; Milk; Postpartum; Metabolites.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000300652

Insulin effect during a Heatsynch protocol in dairy cows
Provedor de dados: Ciência Rural
Autores: Maffi,Andressa Stein; Brauner,Cássio Cassal; Mielke,Lais Fernanda; Lima,Marcio Erpen; Xavier,Eduardo Gularte; Pino,Francisco Augusto Burkert Del; Corrêa,Marcio Nunes.
ABSTRACT:  The objective of this study was to evaluate the effect of exogenous insulin administration on follicular growth, estrus presentation and conception rate during a protocol of ovulation synchronization. Dairy cows were subjected to the Heatsynch protocol, with the insertion of an intravaginal device containing 1.9 g of progesterone (CIDR) and an intramuscular injection containing 2.5 mg of GnRH on day 0. On day 7, the CIDR was removed and subjects were given 12.5 mg of dinoprost. Also on day 7, Insulin Group (IG, n = 21) animals received a subcutaneous injection containing 0.25 IU / kg of recombinant human insulin and the control group (CG, n = 25) received a 0.9% NaCl injection. On day 8, an injection of 0.5 mg of estradiol cypionate was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy cow; Insulin; Ovarian follicle; Reproduction.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800650

Transient bilateral diabetic cataracts in a Brazilian Terrier puppy
Provedor de dados: Ciência Rural
Autores: Campos,Carla de Freitas; Ferreira,Lilian Stefanoni; Sousa,Marlos Gonçalves; Gama,Fernanda Gomes Velasque; Laus,José Luiz; Carciofi,Aulus Cavalieri.
A case of a Brazilian Terrier puppy presenting diabetic lens opacity that restored transparency after insulin therapy and control of blood glucose levels is reported. This entity has been rarely reported in human beings and has not been reported in dogs before. The rapid glycemic control may have been responsible for the transparency recovery.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transient cataracts; Juvenile diabetes; Diabetes mellitus; Dog; Insulin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000300036