Consulta: subjectFacets:"Micronuclei"
Registros recuperados: 28
Data/hora: 05/06/2024 18:18:07

Micronuclei formation in liver fibrosis samples from patients infected by hepatitis C virus
Provedor de dados: Genetics and Molecular Biology
Autores: Almeida,Terezinha M.B. de; Leitão,Regina Maria C.; Carrilho,Flair J.; Sonohara,Shigueko.
Genetic research on fibrosis outset and its progression in chronic hepatitis (CH) by hepatitis C virus (HCV) are limited. The lack of cytogenetic data led us to investigate the presence of micronuclei (MNi), as a sign of genomic damage. Hepatocytes of hepatic parenchyma from 62 cases diagnosed with CH associated with HCV and displaying different degrees of fibrosis (F1-F4) were analyzed. These data were compared to 15 cases without fibrosis (F0). Twelve healthy liver parenchyma samples were included as control. All samples were obtained from paraffin-embedded archival material. Micronucleated hepatocytes (MN-Heps) were analyzed through Feulgen/Fastgreen staining. Results showed that the rates of MN-Heps in the F4 group were statistically significant (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronuclei; Hepatocytes; Chronic hepatitis C; Fibrosis progression; HCV-patients.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300003

Chemoprotective effect of cysteamine against the induction of micronuclei by methyl methanesulfonate and cyclophosphamide
Provedor de dados: Genetics and Molecular Biology
Autores: Santos-Mello,Renato; Deimling,Luiz Irineu; Lauer Júnior,Claudio; Carvalho,Thaís Rieger de.
Cysteamine or 2-mercaptoethylamine (MEA) is an aminothiol with a well-known radioprotective action. No specific information is available in the literature about the possible chemoprotective action of MEA against genotoxic chemical agents. This paper presents the results of studies on the ability of MEA to protect mouse bone marrow polychromatic erythrocytes against the induction of micronuclei by alkylating agents such as methyl methanesulfonate (MMS) and cyclophosphamide (CP). We observed that MEA administered intraperitoneally 30 min before or 30 min after the administration of MMS or CP significantly reduced the frequency of micronucleated polychromatic erythrocytes (MNPCEs) induced by the alkylating agents. When MEA was administered in combination with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cysteamine; MEA; Micronuclei; Amifostine; WR-2721.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100027

Genotoxicity evaluation of domestic sewage in a municipal wastewater treatment plant
Provedor de dados: Genetics and Molecular Biology
Autores: Grisolia,Cesar Koppe; Oliveira,Adriano Barros Barata de; Bonfim,Helder; Klautau-Guimarães,Maria de Nazaré.
An evaluation of the genotoxicity of domestic sewage was carried out in a municipal wastewater treatment plant. The study was carried out using Allium cepa root tip cells through cytological parameters such as aberrant cells in anaphase-telophase and the mitotic index, and the fish species Oreochromis niloticus and Tilapia rendalli, through the index of micronuclei in peripheral erythrocytes. In the Allium test, each of the four stages of the wasterwater treatment routine was analyzed, i.e., crude sewage, primary effluent, secondary effluent, and tertiary effluent. O. niloticus and T. rendalli specimens were maintained in an aquarium continuously receiving the final effluent, in an open system, before being discharged into Lake Paranoá. For micronuclei...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Biomonitoring; Domestic sewage; Fish; Micronuclei; Mutagenicity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200026

Genotoxicity of titanium dioxide nanoparticles and triggering of defense mechanisms in Allium cepa
Provedor de dados: Genetics and Molecular Biology
Autores: Santos Filho,Ronaldo dos; Vicari,Taynah; Santos,Samuel A.; Felisbino,Karoline; Mattoso,Ney; Sant’Anna-Santos,Bruno Francisco; Cestari,Marta Margarete; Leme,Daniela Morais.
Abstract Titanium dioxide nanoparticles (TiO2NPs) are widely used and may impact the environment. Thus, this study used a high concentration of TiO2NP (1000 mg/L) to verify the defense mechanisms triggered by a plant system – an indicator of toxicity. Furthermore, this study aimed at completely characterizing TiO2NP suspensions to elucidate their toxic behavior. TiO2NPs were taken up by meristematic cells of Allium cepa, leading to slight inhibition of seed germination and root growth. However, severe cellular and DNA damages were observed in a concentration-dependent manner (10, 100, and 1000 mg/L). For this reason, we used the highest tested concentration (1000 mg/L) to verify if the plant cells developed defense mechanisms against the TiO2NPs and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal aberrations; Micronuclei; Nucleolar alterations; Cellular alterations; Nanoparticle phase-change.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300425

Investigations on DNA damage and frequency of micronuclei in occupational exposure to electromagnetic fields (EMFs) emitted from video display terminals (VDTs)
Provedor de dados: Genetics and Molecular Biology
Autores: Lakshmi,NK; Tiwari,R; Bhargava,SC; Ahuja,YR.
The potential effect of electromagnetic fields (EMFs) emitted from video display terminals (VDTs) to elicit biological response is a major concern for the public. The software professionals are subjected to cumulative EMFs in their occupational environments. This study was undertaken to evaluate DNA damage and incidences of micronuclei in such professionals. To the best of our knowledge, the present study is the first attempt to carry out cytogenetic investigations on assessing bioeffects in personal computer users. The study subjects (n = 138) included software professionals using VDTs for more than 2 years with age, gender, socioeconomic status matched controls (n = 151). DNA damage and frequency of micronuclei were evaluated using alkaline comet assay...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electromagnetic fields (EMFs); Video display terminals (VDTs); Comet assay; DNA damage; Micronuclei.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100026

Cytogenotoxicity biomarkers in fat snook  Centropomus parallelus  from Cananéia and São Vicente estuaries, SP, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Kirschbaum,Aline A.; Seriani,Robson; Pereira,Camilo D. S.; Assunção,Andrea; Abessa,Denis M. de Souza; Rotundo,Matheus M.; Ranzani-Paiva,Maria J. T.
The aquatic environment receives many contaminants that can induce damages at the molecular, biochemical, cellular and physiological levels.  Centropomus parallelus , an important food resource for local populations, is a predator fish that feeds on small fishes and benthic invertebrates, thus being vulnerable to the bioconcentration and biomagnification processes. This study aimed to evaluate cytogenotoxic responses in erythrocytes from  C. parallelus  juveniles collected in the Cananéia and São Vicente estuaries, both in winter and in summer. After anesthesia, blood samples were collected by caudal puncture. Blood smears were prepared on glass slides and stained with May-Grünwald-Giemsa dye. Two thousand cells were analyzed per slide (1000x), and nuclear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenotoxicity; Nuclear abnormalities; Micronuclei; Centropomus parallelus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100023

Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells
Provedor de dados: Genetics and Molecular Biology
Autores: Guterres,Zaira da Rosa; Mantovani,Mário Sérgio; Eira,Augusto Ferreira da; Ribeiro,Lúcia Regina; Jordão,Berenice Quinzani.
Agaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 -&gt; 6)beta-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN) and comet (single cell microgel electrophoresis) assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB) obtained from commercial sources or the following strains: a) strains AB 97/29 (young and sporulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus blazei; Micronuclei; Comet assay; V79 cells; Mushroom extracts.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300022

Dose assessment by quantification of chromosome aberrations and micronuclei in peripheral blood lymphocytes from patients exposed to gamma radiation
Provedor de dados: Genetics and Molecular Biology
Autores: Silva-Barbosa,Isvânia; Pereira-Magnata,Símey; Amaral,Ademir; Sotero,Graça; Melo,Homero Cavalcanti.
Scoring of unstable chromosome aberrations (dicentrics, rings and fragments) and micronuclei in circulating lymphocytes are the most extensively studied biological means for estimating individual exposure to ionizing radiation (IR), which can be used as complementary methods to physical dosimetry or when the latter cannot be performed. In this work, the quantification of the frequencies of chromosome aberrations and micronuclei were carried out based on cytogenetic analyses of peripheral blood samples from 5 patients with cervical uterine cancer following radiotherapy in order to evaluate the absorbed dose as a result of partial-body exposure to 60Co source. Blood samples were collected from each patient in three phases of the treatment: before...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome aberrations; Micronuclei; Biodosimetry; Partial-body irradiation; Lymphocyte.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300021

L'éthoxyrésorufine-o-déethylase, les adduits à l'ADN et les mocronuclei dans les organismes marins. Application à la surveillance des effets biologiques sur les côtes françaises
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Burgeot, Thierry.
Three biomarkers [ethoxyresorufm-o-deethylase (EROD), DNA adducts and micronuclei (MN) were studied for biomonitoring applications along French coasts. EROD was developed at an experirnental stage in two pilot sites, Seine Bay and Fos sur Mer, using the dab (Limanda limanda), dragon et (Callionymus lyra), red mullet (Mullus barbatus) and comber (Serranus hepatus) as target species. Higbest EROD activity was observed in the subestuarial part of Seine Bay and at the stations closest to sources of pollution from Cortiou and Fos sur Mer. The suitable number of fish for detection of a significant difference between two EROD activities was deterrnined as a function of sampling variance and a preselected statistical power. A preliminary study on hepatocytes in a...
Tipo: Text
Palavras-chave: Ethoxyrésorufine-o-déethylase; Adduits à l'ADN; Micronuclei; Poisson; Moule; Huître; Biosurveillance; Ethoryresorufin-o-deethylase; DNA adducts; Micronuclei; Fish; Mussel; Oyster; Biomonitoring.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00443/55462/56984.pdf

Cytological and cytogenic effects induced by thiabendazole on Allium cepa root meristem
Provedor de dados: Journal of Basic and Applied Genetics (BAG)
Autores: Andrioli,Nancy B; Mudry,Marta D.
The toxicity and genotoxicity of thiabendazole (TBZ) in its commercial formulation Foldan® was studied in Allium cepa meristematic cells at concentrations ranging between 10 and 250 µg / ml. Our results show that exposure to TBZ induces a significant increase in the frequency of anaphase telophase chromosomal aberrations, micronuclei and binucleated cells at all the concentrations of TBZ assayed. The frequency of anaphase telophase aberrations was observed to reach a plateau at the concentration of 50 µg / ml of TBZ, which could be probably due to TBZ mitotic toxicity manifested at concentrations of 100 and 250 µg / ml. An increase was also observed in the rate of metaphase and anaphase, which was, in turn, indicative of alterations in chromatid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Cytotoxicity; Mitotic index; Micronuclei; Binucleates; Thiabendazole.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332011000200002

Genotoxic effect of high nitrate water consumption in men and woman from northern Mar del plata, Argentina
Provedor de dados: Journal of Basic and Applied Genetics (BAG)
Autores: Poli,M.N; López Miranda,L.A; Fernández Iriarte,P.J; Zanier,G.J; Iúdica,C.E.
Previous research has shown connections between the exposure to different agents and mutations in germinal and somatic cells. One of such agents, nitrate (NO3), can be potentially genotoxic for individuals who drink high nitrate well water, and may cause a subsequent impact on their health and on the whole ecosystem. The aim of this research was to conduct genotoxicity assays in somatic cells from people living in the northern area of Mar del Plata city, Argentina. The purpose of these experiments was, in turn, to establish the possible relationships between mutagenic effects and high nitrate water consumption. To this end, different diagnostic tests were carried out to detect potential genetic and/or chromosomal alterations. A non-exposed population equal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutagenic agents; Nitrate; Chromosomal aberrations; Genotoxicity; Micronuclei.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332012000100001

Effects of manganese on fat snook Centropomus parallelus (Carangaria: Centropomidae) exposed to different temperatures
Provedor de dados: Neotropical Ichthyology
Autores: Tuzuki,Bárbara Louise L.; Delunardo,Frederico Augusto C.; Ribeiro,Luciana N.; Melo,Caroliny P. de; Gomes,Levy Carvalho; Chippari-Gomes,Adriana Regina.
ABSTRACT This study evaluates the effects of exposure to manganese (Mn2+) for 96 hours at two different temperatures (24 and 27°C) on juveniles of Centropomus parallelus through the activities of glutathione S-transferase (GST) and catalase (CAT), micronuclei test (MN) and comet assay. The GST activity did not show any significant difference between the groups exposed to Mn2+ and the respective control groups; in contrast, a major increase in the CAT activity was observed at 27°C in the group exposed to Mn2+ compared to the control group. The genotoxic analyses showed that in all animals exposed to Mn2+, the number of red cells with micronuclei increased significantly compared to the respective control groups. There was also a significant increase in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomarkers; DNA damage; Enzymes; Genotoxicity; Metal; Micronuclei.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000400202

Effects of cadmium on root growth, cell division and micronuclei formation in root tip cells of Allium cepa var. agrogarum L
Provedor de dados: Phyton: Revista Internacional de Botánica Experimental
Autores: Wang,QL; Zhang,LT; Zou,JH; Liu,DH; Yue,JY.
The effects of various cadmium (Cd) concentrations (10, 50 and 100 &amp;#956;M) on root growth, cell division and micronuclei formation in root tip cells of Allium cepa var. agrogarum L. were investigated to better understand the processes of Cd-induced apoptosis. The results indicated that 10 &amp;#956;M of Cd had an obviously passive influence on root growth during the 24 h treatment, and that the influence was even more serious with increasing Cd concentrations and duration of treatments. The mitotic index decreased with increasing Cd concentration and duration of treatments. Cadmium induced c-mitosis, chromosome bridges, chromosome stickiness and micronuclei. The frequency of cells with these disorders varied with prolonging the duration of treatments...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa var. agrogarum L; Cadmium (Cd); Chromosome disorder; Micronuclei.
Ano: 2014
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572014000200009

Leukocyte profile of the helmeted manakin, Antilophia galeata (Passeriformes: Pipridae) in a Cerrado forest fragment
Provedor de dados: Revista Brasileira de Zoologia
Autores: Ribeiro,Paulo Vitor Alves; Baesse,Camilla Queiroz; Cury,Márcia Cristina; Melo,Celine de.
ABSTRACT Changes in the amounts and proportions of leukocytes, known as leucocyte profiles, have been documented for several bird species and have been used to measure stress levels in these animals. The present work ascertained the biological and ecological attributes that influence the leukocyte profile of Antilophia galeata (Lichtenstein, 1823), the helmeted manakin. This species has been deemed useful in ecological studies because it responds to environmental changes. Blood samples drawn from 89 individuals of A. galeata captured in a Cerrado forest fragment were subjected to analysis under optical microscopy to identify and quantify leukocytes and micronuclei. The number of lymphocytes was greater for males, non-reproductive individuals and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian hematology; Brazilian birds; Micronuclei; Stress; White blood cells.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702020000100321

Genotoxic effects of the diesel water-soluble fraction on the seahorse Hippocampus reidi (Teleostei: Syngnathidae) during acute exposure
Provedor de dados: Revista Brasileira de Zoologia
Autores: Santos,Celina Alcoforado; Novaes,Larissa Simões; Gomes,Levy Carvalho.
The high toxicity of diesel components makes oil spills a threat to the biota in coastal marine environments. The genotoxic effect of the diesel water-soluble fraction (DWSF) on Hippocampus reidi (Ginsburg, 1933) was assessed. Fish were exposed to three different DWSF dilutions for up to 96 hours, and genotoxicity was analyzed using the micronuclei test and the comet assay. The micronuclei test revealed no significant differences between any of the DWSF dilutions and the control in the 24-hours period; however, micronuclei increased in fish exposed to 1:500 and 1:100 DWSF dilutions for 96 hours. For all dilutions, there was an increase in micronuclei in fish exposed for 96 hours when compared to those exposed for 24 hours. The tested dilutions increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Diesel; Marine fish; Micronuclei; Toxicity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702010000600017

Evaluation of antimutagenic and cytotoxic activity of skin secretion extract of Rhinella marina and Rhaebo guttatus (Anura, Bufonidae)
Provedor de dados: Acta Amazonica
Autores: OLIVEIRA,Angellica Fernandes de; CASTOLDI,Lindsey; VIEIRA JUNIOR,Gerardo Magela; MONÇÃO FILHO,Evaldo dos Santos; CHAVES,Mariana Helena; RODRIGUES,Domingos de Jesus; SUGUI,Marina Mariko.
ABSTRACT The skin secretion from toads of the Bufonidae family has great potential in the search for new active compounds to be used as drug candidates in treating some diseases, among them cancer. In this context, this study aimed to evaluate the cytotoxic and antimutagenic activity of the parotoid gland secretion extracts of Rhinella marina and Rhaebo guttatus, as well as biochemically analyze transaminases and serum creatinine for liver and renal damage, respectively. Cytotoxicity was performed by the colorimetric method based on MTT (3- [4, 5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide) with different concentrations of the extracts in Walker or splenic tumor cell cultures from rats and mice. The micronucleus test was performed with male...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Bioprospecting; Micronuclei; Chemoprevention.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000200145

Induced CYP1A activity and DNA damage in fish from the middle Paraíba do Sul River basin, southeastern Brazil
Provedor de dados: Acta Scientiarum: Biological Sciences
Autores: Morado, Claudio Nona; Martins, Thiago Estevan Parente; Araujo, Francisco Gerson; Paumgarten, Francisco José Roma; Gomes, Iracema David.
The Paraíba do Sul River (PSR) drainage basin in Southeastern Brazil covers one of the most industrialized and densely populated regions of the country. The impact of chemical contamination on the PSR basin seems to be more pronounced in its middle reach where a number of potentially polluting plants are located. In this study, we used hepatic EROD activity - a biomarker of exposure to CYP1A-inducing pollutants (e.g. PAHs, PCDD/Fs, PCBs) - and the incidence of micronucleated erythrocytes (Mn) in the peripheral blood - a biomarker of effects of DNA-damaging agents (e.g. PAHs) - to evaluate the effects of pollution on two native fish species, Geophagus brasiliensis and Pimelodus maculatus. Results showed that the incidence of Mn and EROD in G. brasiliensis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecologia aplicada Ethoxyresorufin-O-deethylase (EROD) activity; Geophagus brasiliensis; Micronuclei; Pimelodus maculatus; Polycyclic aromatic hydrocarbons (PAHs). Monitoramento ambiental usando peixes.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36666

Hematology, micronuclei and nuclear abnormalities in fishes from São Francisco river, Minas Gerais state, Brazil - doi: 10.4025/actascibiolsci.v33i1.7117
Provedor de dados: Acta Scientiarum: Biological Sciences
Autores: Seriani, Robson; Instituto de Pesca; Ranzani-Paiva, Maria José Tavares; Instituto de Pesca; Silva-Souza, Ângela Teresa; UEL; Napoleão, Silvia Roseli; Instituto de Pesca.
This study aimed to determine the variables leukocytary, erythrocytary, frequency of micronuclei and nuclear abnormalities in peripheral erythrocytes of Prochilodus argenteus, Pimelodus maculates and Myleus micans from São Francisco river basin, Minas Gerais State, during the summer and winter. Thrombocytes, hematocrit and leukocyte of  P.argenteus series, was influenced by climatic conditions. In P. maculatus, not were significant differences for all leukocytes and thrombocytes. In M. micans, values are unprecedented in the literature, with MCV and absolute number of leukocytes found superior to other species of the family Characidae. The frequency of micronuclei and nuclear abnormalities in erythrocytes, the three species not showed seasonal differences...
Tipo: Estudo ecofisiológico
Palavras-chave: Hematological; Fish; Micronuclei; Nuclear abnormalities; São Francisco Recursos Pesqueiros Hematological; Fish; Micronuclei; Nuclear abnormalities; São Francisco.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7117

Micronúcleos em tétrades de Tradescantia pallida (Rose) Hunt. cv. purpúrea Boom: alterações genéticas decorrentes de poluição aérea urbana - DOI: 10.4025/actascibiolsci.v30i3.5017
Provedor de dados: Acta Scientiarum: Biological Sciences
Autores: Andrade Júnior, Sizenando José de; UESC; Santos Júnior, José Cleub Silva; UNEB; Oliveira, Jesiane da Luz; UNEB; Cerqueira, Eneida de Moraes Marcílio; UEFS; Meireles, José Roberto Cardoso; UEFS.
A atmosfera urbana contém uma mistura de poluentes que inclui substâncias mutagênicas e carcinogênicas. Associação entre estas substâncias e problemas respiratório e cardiovascular, além do aumento de determinados tipos de cânceres, é estabelecida na literatura. Assim, o biomonitoramento é importante para avaliar o potencial genotóxico da atmosfera urbana. O Teste de Micronúcleo em Tradescantia (Trad-MCN) é um dos bioensaios usados para detecção de efeitos genotóxicos. Este teste é baseado na formação de micronúcleos resultante de quebra cromossômica na meiose das células-mãe do grão de pólen de inflorescências de Tradescantia ssp. Neste estudo, Trad-MCN foi utilizado para avaliar o potencial mutagênico do ar atmosférico em duas áreas de tráfego...
Palavras-chave: 2.00.00.00-6    Ciências Biológicas Tradescantia pallida; Micronúcleo; Poluição atmosférica; Biomonitoramento; Genotoxicidade. Tradescantia pallida; Micronuclei; Urban air pollution; Biomonitoring; Genotoxicity.
Ano: 2008
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5017

Protective effects of steroidal alkaloids isolated from Solanum paniculatum L. against mitomycin cytotoxic and genotoxic actions
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: VIEIRA,PABLINE M.; MARINHO,LORENA P.M.; FERRI,SUZANA C.S.; CHEN-CHEN,LEE.
Solanum paniculatum L. is a plant species widespread throughout tropical America, especially in the Brazilian Cerrado region. It is used in Brazil for culinary purposes and in folk medicine to treat liver and gastric dysfunctions, as well as hangovers. Previous studies with S. paniculatum ethanolic leaf extract or ethanolic fruit extract demonstrated that they have no genotoxic activity neither in mice nor in bacterial strains, although their cytotoxicity and antigenotoxicity were demonstrated in higher doses. In order to assess the possible compounds responsible for the activities observed, we fractionated the ethanolic fruit extract of S. paniculatum, characterized by 1H and 13C NMR spectra, and evaluated two fractions containing steroidal alkaloids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticytotoxicity; Antigenotoxicity; Jurubeba; Micronuclei; Solanaceae.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000200553

Evaluation of the mutagenic effect of the iodinated contrast medium Urografina® 292 using the micronucleus test in mouse bone marrow cells
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: BELLE,MONICA B.B.; LEFFA,DANIELA D.; MAZZORANA,DALIANE; ANDRADE,VANESSA M. DE.
Contrast media (CM) are frequently used in diagnostic radiology and in radiotherapy as a diagnostic tool and in treatment planning. Previous studies have demonstrated that these compounds induce chromosomal aberrations. This study evaluates the mutagenic effects induced by the contrast medium Urografina® 292 (meglumine amidotrizoate and sodium-ionic dimmer) in bone marrow cells (BMC) of mice in vivo. Micronuclei assay was performed in BMC of CF-1 mice injected with CM 1.5 and 3.0 mL/kg intravenous doses and 1.0, 2.0, 3.0 mL/kg intraperitoneal doses. The animals were beheaded 24 h after treatment by cervical dislocation, and femur BMC from each animal were used in the micronucleus test. The group treated with the highest intravenous injection of Urografina®...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomes; Contrast medium; Micronuclei; Urografina® 292.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000200737

Effects of atmospheric pollutants on somatic and germ cells of Tradescantia pallida (Rose) D.R. HUNT cv. purpurea
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: CRISPIM,BRUNO A.; SPÓSITO,JULIANA C.V.; MUSSURY,ROSILDA M.; SENO,LEONARDO O.; GRISOLIA,ALEXÉIA B.
Anatomical alterations in leaves and DNA damage in cells caused by the accumulation of atmospheric pollutants can be measured by epidermal leaf analyses and Tradescantia micronuclei assay with early pollen tetrad cells. The present study examined the feasibility of using somatic and germ cells of Tradescantia pallida for biomonitoring purposes in the city of Dourados, state of Mato Grosso do Sul (MS), Brazil. Stomatal, micronucleus and epidermal leaf analyses were performed, using standard methodologies, on plants growing at three locations during six different time periods. Tradescantia micronuclei data were analyzed using SAS 9.2 software package and stomatal data were analyzed using SANEST software. Analyses of stomatal characteristics and micronuclei...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomonitoring; Leaf anatomy; Air pollution; Micronuclei.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401899

Genotoxic effect of Physalis angulata L. (Solanaceae) extract on human lymphocytes treated in vitro
Provedor de dados: Biocell
Autores: Alves dos Santos,Raquel; Cabral,Teresinha Rosa; Cabral,Isabel Rosa; Greggi Antunes,Lusânia Maria; Pontes Andrade,Cristiane; Cerqueira dos Santos Cardoso,Plínio; de Oliveira Bahia,Marcelo; Pessoa,Claudia; Martins do Nascimento,José Luis; Rodríguez Burbano,Rommel; Satie Takahashi,Catarina.
Physalis angulata L (Solanaceae) is a medicinal plant from North of Brazil, whose different extracts and infusions are commonly used in the popular medicine for the treatment of malaria, asthma, hepatitis, dermatitis and rheumatism. However, the genotoxic effects of P. angulata on human cells is not well known. The main purpose of the present study was to evaluate the in vitro genotoxic effects of aqueous extract of P. angulata using the comet assay and the micronucleus assay in human lymphocytes provided from 6 healthy donors. Treatments with P. angulata extracts were performed in vitro in order to access the extent of DNA damage. The comet assay has shown that treatments with P. angulata at 0.5, 1.0, 2.0, 3.0 and 6.0 &amp;#956;g/mL in culture medium were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P. angulata; Micronuclei; Comet assay.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200005

Genotoxicity Evaluation of Dipotassium -Trioxohydroxytetrafluorotriborate, K2(B3O3F4OH), in Human Lymphocyte Cultures and Mice Reticulocytes
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Haveric,Sanin; Hadzic,Maida; Haveric,Anja; Mijanovic,Mirjana; Hadziselimovic,Rifat; Galic,Borivoj.
ABSTRACT Genotoxic effects of inorganic molecule dipotassium-trioxohydroxytetrafluorotriborate, K2(B3O3F4OH), a promising new therapeutic for the epidermal changes treatment, have been evaluated. In vitro analysis included evaluation of genotoxic and cytotoxic potential of K2(B3O3F4OH) in concentrations of 0.01, 0.02, 0.05 and 0.06 mg/mL applying cytokinesis-block micronucleus cytome assay in human lymphocyte culture. With the increase of concentration the frequency of micronuclei elevated but the differences were not significant. Also, there were no significant differences among the frequencies of nuclear buds and nucleoplasmic bridges between controls and treated cultures. Nuclear division index and nuclear division cytotoxycity index values did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Micronuclei; Halogenated boroxine; Human lymphocyte culture; BALB/c mice.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100348

Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Silva,N.P.; Christofolini,D.M.; Mortara,R.A.; Andrade,L.E.C.
Cajal bodies (CB) are ubiquitous nuclear structures involved in the biogenesis of small nuclear ribonucleoproteins and show narrow association with the nucleolus. To identify possible relationships between CB and the nucleolus, the localization of coilin, a marker of CB, and of a set of nucleolar proteins was investigated in cultured PtK2 cells undergoing micronucleation. Nocodazol-induced micronucleated cells were examined by double indirect immunofluorescence with antibodies against coilin, fibrillarin, NOR-90/hUBF, RNA polymerase I, PM/Scl, and To/Th. Cells were imaged on a BioRad 1024-UV confocal system attached to a Zeiss Axiovert 100 microscope. Since PtK2 cells possess only one nucleolus organizer region, micronucleated cells presented only one or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cajal body; Coiled body; Micronuclei; Cell nucleolus; Nucleolus organizer region; RNA polymerase I; Nucleolar proteins.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700008

Micronucleated lymphocytes in parents of Down syndrome children
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Silva-Grecco,R.L.; Navarro,G.C.; Cruz,R.M.; Balarin,M.A.S.
Down syndrome (DS) is the most common disease due to an autosomal aneuploidy in live born children and also the major known genetic cause of mental retardation. The risk of a DS pregnancy increases substantially with increasing maternal age. However, several women aged less than 35 years at conception have a child with DS. The micronucleus (MN) assay can identify chromosome breakage or chromosome malsegregation and is an ideal biomarker to investigate genomic instability. The aim of the present study was to determine the frequency of peripheral lymphocytes with MN in the parents of DS individuals. The subjects were 17 couples, 1 father and 9 mothers, and 24 couples who had at least one healthy child formed the control group. For each individual we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronuclei; Parents of Down syndrome children; Chromosomal nondisjunction; Down syndrome.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700002

Tomato and garlic by gavage modulate 7,12-dimethylbenz[a]anthracene-induced genotoxicity and oxidative stress in mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bhuvaneswari,V.; Velmurugan,B.; Abraham,S.K.; Nagini,S.
Chemoprotection by dietary agents is a promising strategy for cancer prevention. The aim of the present study was to evaluate the combined effect of tomato and garlic against 7,12-dimethylbenz- [a]anthracene (DMBA)-induced genetic damage and oxidative stress in 12-14-week-old male Swiss albino mice. The animals were randomized into experimental and control groups and divided into eight groups of five animals each. Group 1 animals were injected intraperitoneally with 35 mg/kg body weight DMBA suspended in peanut oil as a single dose. Groups 2-4 animals received tomato (500 mg/kg body weight), garlic (125 mg/kg body weight) and a combination of tomato and garlic for 5 days by gavage, respectively, followed by DMBA 1.5 h after the final feeding. The doses of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Antioxidants; DMBA; Garlic; Lipid peroxidation; Micronuclei; Tomato; Lycopene.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700012

Genetic stability in synthetic wheat accessions: cytogenetic evaluation as a support in breeding programs
Provedor de dados: Ciência Rural
Autores: Frizon,Patrícia; Brammer,Sandra Patussi; Lima,Maria Imaculada Pontes Moreira; Castro,Ricardo Lima de; Deuner,Carolina Cardoso.
ABSTRACT: Synthetic wheat is developed by crossing tetraploid species ( Triticum turgidum , AABB) with a diploid species ( Aegilops tauschii , DD), followed by chromosome duplication through the use of colchicine to restore the resultant sterile hybrid to a fertile hexaploid plant. The main importance of producing synthetically improved wheat is to increase their genetic variability and to incorporate genes that code for resistance to biotic and abiotic stressors. This study aimed to evaluate the presence of micronuclei (MN) and the meiotic index (MI) in the tetrad phase in synthetic wheat accessions and cultivars ( Triticum aestivum ) stored at the Germplasm Bank of Embrapa Trigo (Brazil), in order to identify and select genetically stable accessions for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aegilops tauschii; Meiotic index; Micronuclei.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000400401