Consulta: subjectFacets:"Microsatellite markers"
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Data/hora: 11/06/2024 03:32:12

Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces
Provedor de dados: Electronic Journal of Biotechnology
Autores: Pervaiz,Zahida Hassan; Rabbani,M. Ashiq; Khaliq,Ishtiaq; Pearce,Stephen R; Malik,Salman A.
Genetic diversity underlies the improvement of crops by plant breeding. Land races of rice (Oryza sativa L.) can contain some valuable alleles not common in modern germplasm. The aim here was to measure genetic diversity and its effect on agronomic traits among rice land-race genotypes grown in Pakistan. Diversity was measured using thirty-five microsatellite markers and seventy-five genotypes. Among the markers used a total of 142 alleles were detected at 32 polymorphic SSR loci, while three loci were monomorphic in Pakistani rice landraces. The number of alleles identified by each marker ranged from 2 to 13 with a mean of 4.4. Size differences between the smallest and largest alleles varied from 11bp to 71bp. Polymorphism information content ranged from...
Tipo: Journal article
Palavras-chave: Genetic diversity; Landraces; Microsatellite markers; Oryza sativa L.; Pakistan; Rice.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300004

Development, characterization and use of genomic SSR markers for assessment of genetic diversity in some Saudi date palm (Phoenix dactylifera L.) cultivars
Provedor de dados: Electronic Journal of Biotechnology
Autores: Al-Faifi,Sulieman A; Migdadi,Hussein M; Algamdi,Salem S; Altaf Khan,Mohammad; Ammar,Megahed H; Al-Obeed,Rashid S; I. Al-Thamra,Mohammad; EL-Harty,Ehab H; Jakse,Jerenj.
Background: The present study was undertaken towards the development of SSR markers and assessing genetic relationships among 32 date palm (Phoenix dactylifera L.) representing common cultivars grown in different geographical regions in Saudi Arabia. Results: Ninety-three novel simple sequence repeat markers were developed and screened for their ability to detect polymorphism in date palm. Around 71% of genomic SSRs were dinucleotide, 25% tri, 3% tetra and 1% penta nucleotide motives. Twenty-two primers generated a total of 91 alleles with a mean of 4.14 alleles per locus and 100% polymorphism percentage. A 0.595 average polymorphic information content and 0.662 primer discrimination power values were recorded. The expected and observed heterozygosities...
Tipo: Journal article
Palavras-chave: Expected and observed heterozygosity; Microsatellite markers; Polymorphic information content; Polymorphism; Primer discrimination power.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300002

Chromosomal locations of the maize (Zea mays L.) HtP and rt genes that confer resistance to Exserohilum turcicum
Provedor de dados: Genetics and Molecular Biology
Autores: Ogliari,Juliana Bernardi; Guimarães,Marco Antônio; Camargo,Luis Eduardo Aranha.
We used 125 microsatellite markers to genotype the maize (Zea mays L.) near isogenic lines (NIL) L30HtPHtPRtRt and L30htphtpRtRt and the L40htphtprtrt line which contrast regarding the presence of the recently described dominant HtP and the recessive rt genes that confer resistance to Exserohilum turcicum. Five microsatellite markers revealed polymorphisms between the NIL and were considered candidate linked markers for the HtP resistance gene. Linkage was confirmed by bulked segregant sample (BSS) analysis of 32 susceptible and 34 resistant plants from a BC1F1 population derived from the cross (L30HtPHtPRtRt x L40htphtprtrt) x L40htphtprtrt. The bnlg198 and dupssr25 markers, both located on maize chromosome 2L (bin 2.08), were polymorphic between bulks....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bulked segregant sample analysis; Microsatellite markers; Maize; Near-isogenic lines; Northern leaf blight.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400021

Mapping of quantitative trait loci and confirmation of the FAT1 region on chromosome 4 in an F2 population of pigs
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Kleibe de Moraes; Paixão,Débora Martins; Silva,Priscila Vendramini; Solero,Bruna Pena; Pereira,Mario Sérgio; Lopes,Paulo Sávio; Guimarães,Simone Eliza Facioni.
The objective was to map QTL on porcine chromosome 4 and to associate them with carcass and internal organ traits in an F2 population. The F1 population was produced by outbreed crossing, using two native Brazilian breed Piau boars and 18 commercial sows. A total of 617 F2 animals issued from 11 F1 boars and 54 F1 sows were typed for a total of five microsatellite markers. The data were analyzed by multiple regressions developed for the analysis of crosses between outbred lines, using the QTL Express software. Significant evidence for QTL was found for pig chromosome 4 regarding carcass and internal organ traits. All QTL were detected in the same region of the chromosome, designated FAT1.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatness; Pig production; Divergent crosses; Microsatellite markers; QTL mapping.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300013

Assessment of genetic diversity in Brazilian barley using SSR markers
Provedor de dados: Genetics and Molecular Biology
Autores: Ferreira,Jéssica Rosset; Pereira,Jorge Fernando; Turchetto,Caroline; Minella,Euclydes; Consoli,Luciano; Delatorre,Carla Andréa.
Abstract Barley is a major cereal grown widely and used in several food products, beverage production and animal fodder. Genetic diversity is a key component in breeding programs. We have analyzed the genetic diversity of barley accessions using microsatellite markers. The accessions were composed of wild and domesticated barley representing genotypes from six countries and three breeding programs in Brazil. A total of 280 alleles were detected, 36 unique to Brazilian barley. The marker Bmag120 showed the greatest polymorphism information content (PIC), with the highest mean value found on chromosome three, and the lowest on chromosomes four and six. The wild accessions presented the highest diversity followed by the foreign genotypes. Genetic analysis was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Domesticated barley; Genetic diversity; Hordeum vulgare ssp. vulgare; Hordeum vulgare ssp. spontaneum; Microsatellite markers.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100086

Combined association of Presenilin-1 and Apolipoprotein E polymorphisms with maternal meiosis II error in Down syndrome births
Provedor de dados: Genetics and Molecular Biology
Autores: Bhaumik,Pranami; Ghosh,Priyanka; Ghosh,Sujay; Feingold,Eleanor; Ozbek,Umut; Sarkar,Biswanath; Dey,Subrata Kumar.
Abstract Alzheimer's disease and Down syndrome often exhibit close association and predictively share common genetic risk-factors. Presenilin-1 (PSEN-1) and Apolipoprotein E (APOE) genes are associated with early and late onset of Alzheimer's disease, respectively. Presenilin −1 is involved in faithful chromosomal segregation. A higher frequency of the APOE ε4 allele has been reported among young mothers giving birth to Down syndrome children. In this study, 170 Down syndrome patients, grouped according to maternal meiotic stage of nondisjunction and maternal age at conception, and their parents were genotyped for PSEN-1 intron-8 and APOE polymorphisms. The control group consisted of 186 mothers of karyotypically normal children. The frequencies of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome; Genetic polymorphism; Karyotype; Meiosis; Microsatellite markers.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400577

Inheritance and identification of molecular markers associated with spot blotch (Cochliobolus sativus L.) resistance through microsatellites analysis in barley
Provedor de dados: Genetics and Molecular Biology
Autores: Kuldeep,Tyagi; Nandan,Rajiv; Kumar,Uttam; Prasad,Lal Chand; Chand,Ramesh; Joshi,Arun Kumar.
Spot blotch resistant (IBON 18) and susceptible (RD 2508) lines were crossed to investigate inheritance of resistance and to identify simple sequence repeats (SSRs) associated with resistance. F1 resistance was intermediate and suggested additive nature of inheritance. Three additive genes was noted in the distribution of F3, F4 and F5 generations. In F6 and F6-7, the quantitative and qualitative approaches also suggested the control of three resistance genes. The parents and the RILs (F6/F6-7) were grown in four environments and spot blotch severity recorded. Forty five SSR primers, specific for chromosomes 1 (7H) and 5 (1H), were applied. Of these, 12 were polymorphic between the parents, and between the resistant and susceptible bulks. Three markers BMS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite markers; Molecular marker; Spot blotch; Cochliobolus sativus; Barley.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400021

Comparative genome-wide polymorphic microsatellite markers in Antarctic penguins through next generation sequencing
Provedor de dados: Genetics and Molecular Biology
Autores: Vianna,Juliana A.; Noll,Daly; Mura-Jornet,Isidora; Valenzuela-Guerra,Paulina; González-Acuña,Daniel; Navarro,Cristell; Loyola,David E.; Dantas,Gisele P. M.
Abstract Microsatellites are valuable molecular markers for evolutionary and ecological studies. Next generation sequencing is responsible for the increasing number of microsatellites for non-model species. Penguins of the Pygoscelis genus are comprised of three species: Adélie (P. adeliae), Chinstrap (P. antarcticus) and Gentoo penguin (P. papua), all distributed around Antarctica and the sub-Antarctic. The species have been affected differently by climate change, and the use of microsatellite markers will be crucial to monitor population dynamics. We characterized a large set of genome-wide microsatellites and evaluated polymorphisms in all three species. SOLiD reads were generated from the libraries of each species, identifying a large amount of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite markers; Penguin; Genome; Polymorphism; Tetranucleotide.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400676

Characterization of 13 microsatellite loci developed from Meconopsis horridula
Provedor de dados: Genetics and Molecular Biology
Autores: Zhao,Yu; Zhang,Shi-Bao; Yang,Jing; Zhang,Lu.
Meconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic structure; Heterozygosity; Meconopsis horridula; Microsatellite markers; Polymorphism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300026

Genetic diversity and phylogenetic relationships in the rye genus Secale L. (rye) based on Secale cereale microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Shang,Hai-Ying; Wei,Yu-Ming; Wang,Xiao-Rong; Zheng,You-Liang.
The genetic diversity and phylogenetic relationships in the genus Secale L. (rye) was evaluated using 24 Secale cereale microsatellite (SCM) markers. The average polymorphism information content (PIC) value of each microsatellite locus in 30 Secale accessions evaluated was higher than that in 47 cultivated ryes (Secale cereale ssp. cereale). The mean genetic similarity (GS) index in Secale was lower than that in cultivated rye. The highest within-species GS index was observed for S. sylvestre and the lowest for S. strictum, whereas the highest between-species GS index was found between S. cereale and S. vavilovii and the lowest between S. sylvestre and S. cereale. There was no obvious difference in GS levels in the cultivated rye accessions from Asia,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cultivated rye; Genetic diversity; Microsatellite markers; Phylogenetic relationships; Secale L.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400018

Analysis of the association between spawning time QTL markers and the biannual spawning behavior in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: Genetics and Molecular Biology
Autores: Colihueque,Nelson; Cárdenas,Rosy; Ramírez,Lorena; Estay,Francisco; Araneda,Cristian.
The rainbow trout is a salmonid fish that occasionally exhibits broodstocks with biannual spawning behavior, a phenomenon known as a double annual reproductive cycle (DARC). Spawning time quantitative trait loci (SPT-QTLs) affect the time of the year that female rainbow trout spawn and may influence expression of the DARC trait. In this study, microsatellite markers linked and unlinked to SPT-QTLs were genotyped to investigate the underlying genetics of this trait. SPT-QTLs influenced the DARC trait since in two case-control comparisons three linked markers (OmyFGT12TUF, One3ASC and One19ASC) had significant levels of allelic frequency differentiation and marker-character association. Furthermore, alleles of One3ASC and One19ASC had significantly higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Association analysis; Biannual spawning; Microsatellite markers; Rainbow trout.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300032

Quantitative trait locus affecting birth weight on bovine chromosome 5 in a F2 Gyr x Holstein population
Provedor de dados: Genetics and Molecular Biology
Autores: Gasparin,Gustavo; Miyata,Marcelo; Coutinho,Luiz Lehmann; Martinez,Mário Luiz; Silva,Marcos Vinícius G. Barbosa da; Machado,Marco Antônio; Campos,Ana Lúcia; Regitano,Luciana Correia de Almeida.
Segregation between a genetic marker and a locus influencing a quantitative trait in a well delineated population is the basis for success in mapping quantitative trait loci (QTL). To detect bovine chromosome 5 (BTA5) birth weight QTL we genotyped 294 F2 Gyr (Bos indicus) x Holstein (Bos taurus) crossbreed cattle for five microsatellite markers. A linkage map was constructed for the markers and an interval analysis for the presence of QTL was performed. The linkage map indicated differences in the order of two markers relative to the reference map (&lt;A HREF="http://www.marc.usda.gov/"&gt;http://www.marc.usda.gov&lt;/A&gt;). Interval analysis detected a QTL controlling birth weight (p &lt; 0.01) at 69 centimorgans (cM) from the most centromeric marker...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BTA5; Birth weight; Cattle; QTL; Microsatellite markers.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500005

Genetic variability and efficiency of DNA microsatellite markers for paternity testing in horse breeds from the Brazilian Marajó archipelago
Provedor de dados: Genetics and Molecular Biology
Autores: Reis,Sávio P.; Gonçalves,Evonnildo C.; Silva,Artur; Schneider,Maria P.C.
In this study, 15 microsatellite DNA loci used in comparative tests by the International Society for Animal Genetics were applied to the evaluation of genetic diversity and management, and the efficiency of paternity testing in Marajoara horses and Puruca ponies from the Marajó Archipelago. Based on the genotyping of 93 animals, mean allelic diversity was estimated as 9.14 and 7.00 for the Marajoara and Puruca breeds, respectively. While these values are similar to those recorded in most European breeds, mean levels of heterozygosity were much lower (Marajoara 49%, Puruca 40%), probably as a result of high levels of inbreeding in the Marajó populations. The mean informative polymorphic content of this 15-marker system was over 50% in both breeds, and was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Marajoara horses; Puruca ponies; Microsatellite markers; Genetic diversity; Geneaological control.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100014

Effect of natural selection on common bean (Phaseolus vulgaris) microsatellite alleles
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Taislene Butarello; Santos,João Bosco dos.
The effect of natural selection on microsatellite simple sequence repeat (SSR) alleles was investigated in two distinct common bean (Phaseolus vulgaris) generations (F8 and F24) derived from the cross between the P. vulgaris cultivars Carioca MG x ESAL 686. The F2 segregant population was propagated by the bulk method and 107 plants were sampled in two generations (F8 and F24). Each plant generated one family which was replicated by the bulk method to F8:11 and F24:27 families from which DNA was extracted. Thirty pairs of microsatellite primers were polymorphic for the parents and the bulk of the F24:27 families. Out of 30 loci selected by natural selection, 29 microsatellite alleles came from the Carioca MG parent and one allele came from the ESAL 686...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Natural selection; Microsatellite markers; Phaseolus vulgaris; Adaptation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200024

Quantitative trait loci for carcass, internal organ and meat quality traits on porcine chromosomes 16, 17 and 18
Provedor de dados: Genetics and Molecular Biology
Autores: Paixão,Débora M.; Silva Filho,Miguel I. da; Pereira,Mário S.; Lopes,Marcos S.; Barbosa,Leandro; Souza,Katiene Régia Silva; Lopes,Paulo S.; Guimarães,Simone E.F.
The objective of this study was to map quantitative trait loci (QTL) on porcine chromosomes 16, 17 and 18 and to determine their association with carcass, organ and meat quality traits. An F2 population was produced by crossing two boars of the naturalized Brazilian Piau breed with 18 commercial females (Landrace x Large White x Pietrain). The population was genotyped for 11 microsatellite markers distributed over the three chromosomes and the results were used to construct a marker-specific linkage map for the population. Analysis of the polymorphic information content showed that the microsatellite markers were adequate for the study of quantitative traits. QTL were identified by regression interval mapping using QTL Express software. QTL not previously...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Divergent crosses; Microsatellite markers; Pig production; QTL mapping.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500016

Determination of genetic variability of traditional varieties of Brazilian rice using microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Brondani,Claudio; Borba,Tereza Cristina Oliveira; Rangel,Paulo Hideo Nakano; Brondani,Rosana Pereira Vianello.
The rice (Oryza sativa) breeding program of the Rice and Bean research center of the Brazilian agricultural company Empresa Brasileira de Pesquisa Agropecuária (Embrapa) is well established and provides new cultivars every year to attend the demand for improved high yielding varieties with tolerance to biotic and abiotic stresses. However, the elite genitors used to compose new populations for selection are closely related, contributing to the yield plateau reached in the last 20 years. To overcome this limit, it is necessary to broaden the genetic basis of the cultivars using diverse germplasm such as wild relatives or traditional varieties, with the latter being more practical because they are more easily crossed with elite germplasm to accelerate the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Genetic resources; Microsatellite markers; Oryza sativa.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400017

Evaluation of genetic variability in the collared peccary Pecari tajacu and the white-lipped peccary Tayassu pecari by microsatellite markers
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Roxane Wirschum; Freitas,Thales R.O. de; Sbalqueiro,Ives José.
In this study, the microsatellite technique was used to evaluate the genetic variability in populations of collared and white-lipped peccaries kept in captivity. Six primers developed for domestic pigs were used and amplified in both species. They revealed the presence of five polymorphic loci and one monomorphic locus. The polymorphic loci included 4 of the 16 alleles in collared peccaries, and 3 of the 10 alleles in the white-lipped peccaries. Polymorphic information content (PIC) in both species and all the loci was highly informative. The probability of paternity exclusion (PEC), if one of the parents is known, was almost as high in white-lipped peccaries (95.53%) as in the collared (99,48%). The Fst values for collared (0.042) and white-lipped...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite markers; Tayassu pecari; Pecari tajacu; Genetic variability; Animals of captivity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100012

SNP discovery and characterisation in White Rhino (Ceratotherium simum) with application to parentage assignment
Provedor de dados: Genetics and Molecular Biology
Autores: Labuschagne,Christiaan; Dalton,Desiré L.; Grobler,J. Paul; Kotzé,Antoinette.
Abstract The white rhino is one of the great success stories of modern wildlife conservation, growing from as few as 50-100 animals in the 1880s, to approximately 20,000 white rhinoceros remaining today. However, illegal trade in conservational rhinoceros horns is adding constant pressure on remaining populations. Captive management of ex situ populations of endangered species using molecular methods can contribute to improving the management of the species. Here we compare for the first time the utility of 33 Single Nucleotide Polymorphisms (SNPs) and nine microsatellites (MS) in isolation and in combination for assigning parentage in captive White Rhinoceros. We found that a combined dataset of SNPs and microsatellites was most informative with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single nucleotide polymorphisms; Microsatellite markers; White Rhinoceros; Ceratotherium simum.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100084

Genetic variability and selective breeding for traits of aquacultural interest in the Pacific oyster (Crassostrea gigas) (slides)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno.
To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained in diploids using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. Recently, in the U.S.A., Australia and New Zealand, countries where hatcheries are a major source of C. gigas juveniles, family-based selective...
Tipo: Text
Palavras-chave: Microsatellite markers; Selective breeding; Genetic; Genetic variability; Crassostrea gigas; Pacific oysters.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3449.pdf

Génotypage des géniteurs de Lates calcarifer de Tahiti : aide à la domestication raisonnée du Loup Tropical pour la filière tahitienne
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Vonau, Vincent; Rouxel, Catherine; Saulnier, Denis; Cochennec-laureau, Nathalie; Nedelec, Georges; Goyard, Emmanuel.
The tropical seabass Lates calcarifer was introduced in Tahiti in 1984 and then was domesticated without other introduction. Three successive generations have been obtained in captivity. Tissue samples of the 38 animaIs which represent the total Tahitian broodstock were preserved in alcohol to be genotyped with four microsatellite markers (Yue et al., 2001). Three of four described markers were successfully transferred to the laboratory of genetics of Tahiti (marker LcaM01, LcaM02, LcaM03), as the revelation technology available locally did not allow the use of LcaM04 in routine. The results show that the genetic diversity of the tahitian broodstock is not equivalent of the one of the species, which could be explained by the small size of the founder...
Tipo: Text
Palavras-chave: Lates calcarifer; Marqueurs microsatellites; Reproducteurs; Variabilité génétique; Lates calcarifer; Microsatellite markers; Breeding stock; Genetic diversity.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00142/25300/23372.pdf

Genetic improvement of hatchery propagated bivalve stocks : prospects and constraints
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boudry, Pierre.
To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the production of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or other traits, could be obtained in diploids. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. In the U.S.A., Australia and New Zealand, family-based selective breeding programs have recently been initiated to improve growth, disease tolerance and yield.  In Europe, where both natural and...
Tipo: Text
Palavras-chave: Microsatellite markers; Crassostrea gigas; Selective breeding; Triploid; Pacific oyster; Genetic.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3451.pdf

Isolation of 12 microsatellite markers following a pyrosequencing procedure and cross-priming in two invasive cryptic species, Alexandrium catenella (group IV) and A. tamarense (group III) (Dinophyceae)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Laporte, Martin; Shao, Zhaojun; Berrebi, Patrick; Laabir, Mohamed; Abadie, Eric; Faivre, Nicolas; Rieuvilleneuve, Fabien; Masseret, Estelle.
Alexandrium catenella (group IV) and Alexandrium tamarense (group III) (Dinophyceae) are two cryptic invasive phytoplankton species belonging to the A. tamarense species complex. Their worldwide spread is favored by the human activities, transportation and climate change. In order to describe their diversity in the Mediterranean Sea and understand their settlements and maintenances in this area, new microsatellite markers were developed based on Thau lagoon (France) samples of A. catenella and A. tamarense strains. In this study twelve new microsatellite markers are proposed. Five of these microsatellite markers show amplifications on A. tamarense and ten on A. catenella. Three of these 12 microsatellite markers allowed amplifications on both cryptic...
Tipo: Text
Palavras-chave: Invasive species; Phytoplankton; Alexandrium; Dinophyceae; Microsatellite markers; Biogeography.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00189/30026/29398.pdf

Towards the development of clonal lines in the European sea bass (Dicentrarchus labrax L.): application of uniparental reproduction techniques with an insight into sea bass eggs
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Colleter, Julie.
Clonal lines are a powerful scientific tool for improved genetic characterization of organisms used in research. Inbred fish lines can be produced in only two generations using uniparental reproduction techniques. Androgenesis, achieved with variable success in several freshwater species, has been attempted in the European sea bass (Dicentrarchus labrax L), a marine fish of commercial and scientific interest. The low yields of progenies inheriting only the paternal genome after UV-irradiation of eggs led to considerations on the occurrence of UV screening compounds in pelagic eggs. Mycosporine-like amino acids and gadusol were found in many marine and freshwater organisms, but their occurrence in fish eggs was not clearly related to a behavioral pattern...
Tipo: Text
Palavras-chave: Dicentrarchus labrax; Lignées clonales; Androgenèse; Gynogenèse; MAAs; Gadusol; Marqueurs microsatellites; Homozygotie; Hétérozygotie; Taux de recombinaison.; Dicentrarchus labrax; Clonal lines; Androgenesis; Gynogenesis; MAAs; Gadusol; Microsatellite markers; Homozygosity; Heterozygosity; Recombination rate.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00300/41138/40326.pdf

Genetic impact of the reproduction dynamics in the European flat oyster Ostrea edulis
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lapegue, Sylvie; Taris, Nicolas; Lallias, Delphine; Bonhomme, Francois; Boudry, Pierre.
The European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. The latest results obtained on the genetic differentiation between these populations have led us to persue studies at a finer scale, in order to estimate the effective number of breeders and the temporal dynamics of reproduction and, more specially, recruitment. Several experiments were performed to document (1) the variance in allele frequencies during a natural settlement period, (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females, (3) the variance of individual...
Tipo: Text
Palavras-chave: Recruitment; Microsatellite markers; Ostrea edulis; European flat oyster; Genetic.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3485.pdf

Mitochondrial and microsatellite genetic differentiation of the flat oyster Ostrea edulis along the european coasts, and comparison with allozyme data
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lapegue, Sylvie; Diaz-almela, E.; Launey, Sophie; Ledu, Christophe; Boudry, Pierre; Naciri-graven, Yamama; Bonhomme, Francois.
Natural populations of the European flat oyster, Ostrea edulis, have been harvested since Roman times. This species is distributed along the European Atlantic coasts, from Norway to Morocco, as well as the Mediterranean Sea and Black sea coasts. Previous studies, based on allozymes, have concluded that isolation by distance is taking place in  the Mediterranean sea and the Atlantic ocean. Because of the presumed non neutrality of these markers, another approach was made to examine the genetic differentiation of this species using nuclear microsatellite and mitochondrial markers, both considered as neutral, and to compare the results to those observed with allozymes.
Tipo: Text
Palavras-chave: Microsatellite markers; Ostrea edulis; Flat oyster; Genetic.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/acte-3497.pdf

Microsatellite markers as a tool to study reproductive success in the Pacific oyster, Crassostrea gigas (Thunberg), under controlled hatchery conditions
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Bonhomme, Francois.
Oysters, like many marine species have a very high fecundity. Previous studies have shown that populations, from both hatcheries and the natural environment, have very low Ne/N ratios. These observations reveal high variation in reproductive success. In order to study individual reproductive success under controlled conditions, we used microsatellite markers to quantify parental contributions in in vitro crosses (5 males and 5 females) of  Crassostrea gigas,  the Pacific oyster. High polymorphism of the microsatellites (more that 50 alleles per locus) eased the parentage identifications. The results of a cross allowing gametic competition were compared with the results from a second cross where the gametes of the same parents were kept separate for each...
Tipo: Text
Palavras-chave: Genetic; Hatchery; Microsatellite markers; Crassostrea gigas; Pacific oysters.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/acte-3457.pdf

High connectivity across the fragmented chemosynthetic ecosystems of the deep Atlantic Equatorial Belt: efficient dispersal mechanisms or questionable endemism?
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Teixeira, Sara; Olu, Karine; Decker, Carole; Cunha, Regina L.; Fuchs, Sandra; Hourdez, Stephane; Serrao, Ester A.; Arnaud-haond, Sophie.
Chemosynthetic ecosystems are distributed worldwide in fragmented habitats harbouring seemingly highly specialized communities. Yet, shared taxa have been reported from highly distant chemosynthetic communities. These habitats are distributed in distinct biogeographical regions, one of these being the so-called Atlantic Equatorial Belt (AEB). Here, we combined genetic data (COI) from several taxa to assess the possible existence of cryptic or synonymous species and to detect the possible occurrence of contemporary gene flow among populations of chemosynthetic species located on both sides of the Atlantic. Several Evolutionary Significant Units (ESUs) of Alvinocarididae shrimp and Vesicomyidae bivalves were found to be shared across seeps of the AEB. Some...
Tipo: Text
Palavras-chave: Atlantic equatorial belt; Chemosynthetic habitats; Deep-sea connectivity; Endemic bivalves; Endemic shrimp; Genetic diversity; Microsatellite markers; Mitochondrial COI gene.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00157/26789/24950.pdf

Evolutionary dynamics of Cucurbita argyrosperma from the Mesoamerican domestication center using SSR molecular markers
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Priori,Daniela; Villarreal,Daniel Zizumbo; Ek,Víctor Manuel de Jesús Canché; Limones-Briones,Verónica; Barbieri,Rosa Lía.
Abstract: The objective of this work was to evaluate the evolutionary dynamics of the wild-weedy-domestic gene pool of Cucurbita argyrosperma squash by estimating the levels of genetic diversity and gene flow in the putative area of its domestication. Nine populations were collected, and DNA was extracted from young leaves harvested separately from approximately 20 individuals in each population. The DNA fragments were amplified with eight pairs of SSR primers and separated by electrophoresis in 5% denaturing polyacrylamide gels. Genetic diversity and the amount of gene flow were estimated in the populations, and Bayesian grouping was used to determine the levels of gene infiltration and probability of ancestry. The ethnobotanical exploration indicated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cucurbitaceae; Genetic diversity; Microsatellite markers; Plant genetic resources; Squash.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000300287

Genetic similarity in a hybrid population of 'Montenegrina' and 'King' mandarins
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Brugnara,Eduardo Cesar; Bastianel,Marinês; Weiler,Roberto Luis; Schwarz,Sergio Francisco.
The objective of this work was to confirm the hybrids obtained in plants originated from the crossing between the mandarins Citrus deliciosa 'Montenegrina' and C. nobilis 'King', and to estimate the genetic similarity among hybrids, and between each hybrid and its parents. Twenty‑three pairs of microsatellite primers were tested. Fourteen of these pairs showed polymorphic bands between parents. Primers CCSM 129 and CCSME 52 were sufficient to identify the 12 nucellar clones observed in the studied population. Genetic similarity analysis of the population (hybrids and parents) showed 0.56 average similarity. Besides the 12 clones of 'Montenegrina' identified, 25 hybrids were found of which D18, C32, D06, C05 and D09 are the more similar to 'Montenegrina'.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus deliciosa; Citrus nobilis; Hybrid identification; Microsatellite markers.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000800010

Interactions Between Non-Allelic Loci and Their Effects on Categorized Meat Performance Traits in Ducks
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Dobek,A; Gornowicz,E; Moliński,IA; Grajewski,B; Lisowski,M; Szwaczkowski,T.
ABSTRACT Traits associated with meat performance are characterized by a complex inheritance model, including the effects of non-allelic interactions. The aim of this study was to estimate the significance of non-allelic interactions between pairs of loci in each of two alternative groups based on four important performance traits of ducks: body weight (BW), carcass yield calculated as the ratio of carcass weight to body weight (CY), breast and leg muscle yield as a fraction of carcass weight (BL-CW), and skin weight and abdominal fat, again as a fraction of carcass weight (SF-CW). The experimental material was based on an F2 cross between two parental lines: A-55 (Polish Peking) and GL-30 (French Peking). In total, 368 individuals were recorded. All of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Duck; Categorized traits; Entropy analysis; Microsatellite markers.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000200301

Successful Identification of Duck Genome Region Determining Desirable Uniformity of Meat Performance Traits
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Dobek,A; Gornowicz,E; Moliński,K; Grajewski,B; Lisowski,M; Szwaczkowski,T.
ABSTRACT The objective of this study was to identify genome regions determining duck meat performance traits with possible small variation. In total, 368 crossbred ducks of F2 generation obtained from two parental lines: Pekin-type ducks of Polish origin (A55) and Pekin-type ducks of French origin (GL-30) were recorded. The following seven traits were analyzed: body weight, breast muscle weight, leg muscle weight, water holding capacity in the breast and leg muscles, and color lightness L* of the breast and leg muscles. All birds (including parental and F1 generations) were genotyped (29 microsatellite markers). Means and coefficients of variation (CV) were calculated for 28 full-sibs (four sires by six dams and one sire by four dams). Number of progeny...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Duck; Meat quality; Microsatellite markers.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300437

A microsatellite library for Carica papaya L. cv. Sunrise solo
Provedor de dados: Revista Brasileira de Fruticultura
Autores: Santos,Silvia Correa; Ruggiero,Carlos; Silva,Cristina Lacerda Soares Petrarolha; Lemos,Eliana Gertrudes Macedo.
In experimental areas of the Education and Researches Ilha Solteira and Jaboticabal UNESP/Campus farms were selected and tagged 20 hermaphrodite plants and 20 feminine of cultivar Sunrise Solo, Improved Sunrise Solo cv.72/12 and Baixinho of Santa Amália.The seeds origined of the selected fruits were cropped to be analysed the self-pollination efficiency and frequency of the sex in the progenies. After that, samples of the young leaf of the matrix plants were colected for the extration of the DNA. It was built five library enriched of microsatellite sequencies, using probes (TCA)10, (TC)13, (GATA)4, (CAC)10 e (TGAG)8.It was possible the development of the primers only in the library that has utilized the probe (TCA)10. This probe allowed the design of 32...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carica papaya; Sex; Microsatellite markers; Library.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452003000200020

Validation of SSR-tetranucleotide multiplex panel for kinship evaluation in tilapia breeding programs
Provedor de dados: Revista Ciência Agronômica (RCA)
Autores: Freitas,Suzane Fonseca; Moreira,Heden Luiz Marques; Souza,Daiane Machado; Moreira,Carla Giovane Ávila; Tavares,Rafael Aldrighi; Dionello,Nelson José Laurino.
ABSTRACT This study aimed to develop PCR assays, reaction combinations, and validation of tetranucleotide SSR loci for tilapia, to minimize the impact of erroneous allele inferences on genotype determination of this marker. Microsatellites containing tetranucleotide repeats were obtained from tilapia genome, version 2.1, avoiding loci in the same linking group. Primers were designed for different fragment sizes, and fluorescence added to each locus. A total of 10 loci were amplified, separately and in combination, and loaded into a single capillary sequencer panel. Alleles were amplified without stutters and easily interpreted. PCR amplifications of DNA repeatedly extracted from samples, and genotyping at different PCR rounds were performed to infer allele...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Genotyping; Microsatellite markers.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000400409

Genetic relationships between Cuban and Brazilian buffaloes (Bubalus bubalis) by microsatellite markers
Provedor de dados: Revista de Salud Animal
Autores: Martínez Marrero,Nadia; da Silva,Elizabete Cristina; Mitat Valdés,Alina; Ponce,Giselle; Benício da Silva,Regina C.; Paes Barbosa,Severino Benone; Uffo Reinosa,Odalys; Gomes Filho,Manoel Adrião.
Researches on genetic relationships among water buffalo populations are useful in supporting conservation decisions and designing of breeding schemes. The aim of this study was to assess the genetic relationships among Cuban Buffalypso/Carabao hybrids and Murrah and Jaffarabadi Brazilian buffaloes using nine cattle microsatellite markers. During the analysis, four loci were excluded due to the presence of null alleles, stuttering and gametic disequilibrium. Mean numbers of alleles and the heterozygosities observed and expected in the three populations were not significantly different. Heterozygosities found were above 60%. All five loci were in Hardy-Weinberg equilibrium. Wright´s index for estimating inbreeding were negative in the three populations. A...
Tipo: Journal article
Palavras-chave: Bubalus bubalis; Buffalypso; Microsatellite markers; Null allele; Genetic relationships.
Ano: 2015
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000300003

A lima bean core collection based on molecular markers
Provedor de dados: Scientia Agricola
Autores: Gomes,Regina Lucia Ferreira; Costa,Marcones Ferreira; Alves-Pereira,Alessandro; Bajay,Miklos Maximiliano; Viana,João Paulo Gomes; Valente,Sérgio Emílio dos Santos; Lopes,Ângela Celis de Almeida; Zucchi,Maria Imaculada; Pinheiro,José Baldin.
ABSTRACT Some germplasm collections have a high number of accessions, which makes it difficult to explore the genetic variability present in the germplasm bank due to the redundancy and the difficulty of detailed analysis of all conserved accessions. Therefore, our study aimed to analyze the genetic diversity of 153 lima bean (Phaseolus lunatus) accessions for the purpose of constructing a core collection. Eleven SSRs were used for this purpose. The 153 lima bean accessions can be represented by low redundancy using a minimum of 34 accessions, thus representing 22 % of the size of the entire germplasm bank. The core collection had a higher Shannon diversity index and expected heterozygosity (1.906 and 0.811, respectively) than those presented by the entire...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germplasm; Genetic diversity; Genetic structure; Microsatellite markers.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201100

Phenotypic and molecular characterization of apple (Malus × domestica Borkh) genetic resources in Greece
Provedor de dados: Scientia Agricola
Autores: Ganopoulos,Ioannis; Tourvas,Nikolaos; Xanthopoulou,Aliki; Aravanopoulos,Filippos A.; Avramidou,Euaggelia; Zambounis,Antonios; Tsaftaris,Athanasios; Madesis,Panagiotis; Sotiropoulos,Thomas; Koutinas,Nikolaos.
ABSTRACT: The phenotypic and genetic analysis of apple (Malus × domestica Borkh) genotypes is essential for breeding species. Information on the morphology and genetic structure of apple offers significant help for germplasm maintenance and selection of suitable material to breed superior cultivars. This study shows the results of an investigation on the morphology and the genetic diversity for 19 apple cultivars, which are preserved in an ex situ collection in Naoussa, Central Macedonia, Greece. Information was recorded over a 5-year period for 47 traits describing plant morphology and phenotype, as well as leaf and fruit quality. Data were analyzed using the principal component analysis (PCA) method. The Euclidean distance metric and the Ward’s...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Genotyping; Microsatellite markers; Multivariate analysis; Phenotyping.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000600509

Genetic linkage maps of chicken chromosomes 6, 7, 8, 11 and 13 from a Brazilian resource population
Provedor de dados: Scientia Agricola
Autores: Ambo,Marcel; Campos,Raquel de Lello Rocha; Moura,Ana Sílvia Meira Tavares; Boschiero,Clarissa; Rosário,Millor Fernandes do; Ledur,Mônica Corrêa; Nones,Kátia; Coutinho,Luiz Lehmann.
A linkage map is essential not only for quantitative trait loci (QTL) mapping, but also for the organization and location of genes along the chromosomes. The present study is part of a project whose major objective is, besides from construction the linkage maps, the whole genome scan for mapping QTL for performance traits in the Brazilian experimental chicken population. Linkage maps of chicken chromosomes 6 to 8, 11 and 13 were constructed based on this population. The population was developed from two generations of crossbreeding between a broiler and a layer line. Fifty-one microsatellite markers were tested, from which 28 were informative: 4, 8, 7, 4 and 5 for chromosomes 6, 7, 8, 11 and 13, respectively. A SNP located in the leptin receptor gene was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallus gallus; Quantitative trait loci (QTL); Genetic maps; Microsatellite markers; Animal breeding.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500001

A NEW WORLD MONKEY MICROSATELLITE (AP74) HIGHLY CONSERVED IN PRIMATES
Provedor de dados: Acta Biológica Colombiana
Autores: OKLANDER,LUCIANA INÉS; STEINBERG3,ELIANA RUTH; DOLORES MUDRY,MARTA.
Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Primates; Microsatellite markers; PCR amplification; Non-invasive sampling; Genomic conservation; Genetic variability.
Ano: 2012
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000100007

Population structure and impact of recurrent selection on popcorn using EST-SSR markers
Provedor de dados: Acta Scientiarum: Agronomy
Autores: Guimarães, Amanda Gonçalves; Amaral Júnior, Antônio Teixeira do; Almeida Filho, Janeo Eustáquio de; Pena, Guilherme Ferreira; Vittorazzi, Cássio; Pereira, Messias Gonzaga.
The success of any recurrent selection program depends on the genetic variability of the evaluated population, which is used to refer to the diversity of existing alleles at many genetic loci. Thus, the goal of the present study was to investigate the impact of recurrent selection across nine cycles of a UENF-14 popcorn population through the analysis of genetic diversity and structure using microsatellite markers (EST-SSRs). Genomic DNA was extracted from young leaves of 25 individuals from each cycle (C0, C1 C2, C3, C4, C5, C6, C7, and C8), totaling 225 samples from the UENF-14 population. Fifty EST-SSR markers were used for the analysis of genetic diversity across the recurrent selection cycles, 16 of which were polymorphic. Thirty-four alleles were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays L. var. Everta; Variability; Microsatellite markers; Heterozygosity; Equilibrium.; Melhoramento Vegetal.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35218

Comparison of multiallelic distances for the quantification of genetic diversity in the papaya - doi: 10.4025/actasciagron.v33i1.11698
Provedor de dados: Acta Scientiarum: Agronomy
Autores: Ramos, Helaine Christine Cancela; Universidade Estadual do Norte Fluminense; Pereira, Messias Gonzaga; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Gonçalves, Leandro Simões Azeredo; Universidade Estadual do Norte Fluminense; Amaral Júnior, Antonio Teixeira do; Universidade Estadual do Norte Fluminense; Scapim, Carlos Alberto; Universidade Estadual de Maringá.
This study aimed to compare multiallelic distances to quantify genetic diversity in papaya. We evaluated forty-three individuals in the S2 generation, from the backcross between F1 (Cariflora x SS783) and Cariflora, and four accessions from the UENF/Caliman Germplasm Bank. Genetic distances used were Smouse and Peakall (1999), Kosman and Leonard (2005) and weighted index. Clustering among genotypes was performed using the hierarchical unweighted pair-group method with arithmetic mean analysis (UPGMA) and projection of the distance on the bidimensional plan. A high correlation between genetic distances was observed; however, through UPGMA group analysis, the distance determined by the weighted index provided the complete separation of 52BC1S2-08, 52BC1S2-29...
Palavras-chave: Carica papaya L.; Microsatellite markers; Cultivar characterization; Cluster analysis Carica papaya L.; Microsatellite markers; Cultivar characterization; Cluster analysis.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/11698

Population structure and impact of recurrent selection on popcorn using EST-SSR markers
Provedor de dados: Acta Scientiarum: Agronomy
Autores: Guimarães, Amanda Gonçalves; Amaral Júnior, Antônio Teixeira do; Almeida Filho, Janeo Eustáquio de; Pena, Guilherme Ferreira; Vittorazzi, Cássio; Pereira, Messias Gonzaga.
The success of any recurrent selection program depends on the genetic variability of the evaluated population, which is used to refer to the diversity of existing alleles at many genetic loci. Thus, the goal of the present study was to investigate the impact of recurrent selection across nine cycles of a UENF-14 popcorn population through the analysis of genetic diversity and structure using microsatellite markers (EST-SSRs). Genomic DNA was extracted from young leaves of 25 individuals from each cycle (C0, C1 C2, C3, C4, C5, C6, C7, and C8), totaling 225 samples from the UENF-14 population. Fifty EST-SSR markers were used for the analysis of genetic diversity across the recurrent selection cycles, 16 of which were polymorphic. Thirty-four alleles were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.01.03.05-9; 2.08.04.00-8 Zea mays L. var. Everta; Variability; Microsatellite markers; Heterozygosity; Equilibrium. Melhoramento Vegetal.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35218

Assessment of EST-SSR markers for genetic analisys on coffee
Provedor de dados: Bragantia
Autores: Missio,Robson Fernando; Caixeta,Eveline Teixeira; Zambolim,Eunize Maciel; Pena,Guilherme Ferreira; Ribeiro,Ana Paula; Zambolim,Laércio; Pereira,Antônio Alves; Sakiyama,Ney Sussumu.
EST-SSR markers were used to investigate the genetic diversity among and within coffee populations, to explore the possibility of their use for fingerprinting of cultivars and to assist breeding programs. Seventeen markers, developed from ESTs (Expressed Sequence Tags) from the Brazilian Coffee Genome Project, were used. All markers showed polymorphism among the genotypes assessed. The average number of allele per primer was 5.1. The highest polymorphisms were found within C. canephora (88.2%) and rust-resistant varieties (35.3%). About 29.4% of the markers differentiated C. arabica from Híbrido de Timor; it was also possible to identify those closest and farthest from C. arabica . The analysis of population-grouped genotypes revealed a 64.0% genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA markers; Coffea sp.; UPGMA; Amova; Microsatellite markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052009000300003

Estimative of genetic parameters in progeny test of Pinus caribaea Morelet var. hondurensis Barret &amp;amp; Golfari by quantitative traits and microsatellite markers
Provedor de dados: Bragantia
Autores: Tambarussi,Evandro Vagner; Sebbenn,Alexandre Magno; Moraes,Mário Luiz Teixeira de; Zimback,Léo; Palomino,Edwin Camacho; mori,Edson seizo.
The aims of this work were to estimate the coefficient of relatedness within families and the genetic parameters for growth related traits in a progeny test from an open-pollinated variety of Pinus caribaea Morelet hondurensis Barret &amp;amp; Golfari, established in Mato Grosso do Sul State, Brazil. The experimental design was the triple 10 x 10 lattice, with 96 families, three replicates, and ten plants per plot. Fourteen years after planting, the trial was measured for the following traits: total height, diameter at breast height (DBH), and true volume. The estimation of coefficients of relatedness within family from microsatellite loci indicated that families are true half-sibs (^r xy = 0.253). Thus, the additive genetic variance (σ2A) can be estimated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pinus; Tree breeding; Genetic parameters; Microsatellite markers; Coefficient of relatedness.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052010000100006

Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding
Provedor de dados: Bragantia
Autores: Miah,Gous; Rafii,Mohd Y.; Ismail,Mohd Razi; Puteh,Adam Bin; Rahim,Harun Abdul; Ashkani,Sadegh; Latif,Abdul.
The BC2F1 population was derived from a cross between rice variety, MR219 (susceptible to blast) and Pongsu Seribu 1 (resistant to blast). The objectives of this research were to know the inheritance pattern of blast resistance and to identify the linked markers associated with blast resistance in BC2F1 population. Sixteen microsatellite markers were found as polymorphic between the parents related to blast resistant genes (Pi-genes). Among the selected blast resistant linked markers, two markers RM6836 and RM8225 showed expected testcross ratio (1:1) for single-gene model in the BC2F1 population with the association between resistant and susceptible progeny. A total of 333-BC2F1 plants were challenged with the most virulent pathotype P7.2 of Magnaporthe...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blast inheritance; Microsatellite markers; BC2F1 population; Rice variety.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052015000100033

Genetic diversity among Brazilian soybean cultivars based on SSR loci and pedigree data
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Priolli,Regina Helena Geribello; Pinheiro,José Baldin; Zucchi,Maria Imaculada; Bajay,Miklos Maximiliano; Vello,Natal Antonio.
In this study, simple sequence repeats (SSR) loci and pedigree data were used to investigate the genetic relationship in a group of 168 Brazilian soybean cultivars. Eighteen SSR loci produced an average of 5.06 alleles and a mean gene diversity of 0.58 for the cultivars studied. Genetic distance (GD) was determined using the modified Roger's Wright distance, and a final dendrogram was in agreement with the cultivar pedigree. A distance matrix based on the coefficient of parentage scores was also generated for the cultivars, which ranged from 0 to 1, with a mean of 0.18, whereas SSR-based genetic similarity (1- GD) ranged from 0.01 to 0.90, with a mean of 0.25. Mantel's Z test showed that the similarity matrices generated from both the data sets were low,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coefficient of parentage; Glycine max (L.) Merr.; Microsatellite markers.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300004

Bayesian analysis of the genetic structure of a Brazilian popcorn germplasm using data from simple sequence repeats (SSR)
Provedor de dados: Chilean Journal of Agricultural Research
Autores: Saavedra,Javier; Silva,Tereza Aparecida; Mora,Freddy; Scapim,Carlos Alberto.
Several studies have confirmed that popcorn (Zea mays L. var. everta) has a narrow genetic basis, which affects the quality of breeding programs. In this study, we present a genetic characterization of 420 individuals representing 28 popcorn populations from Brazilian germplasm banks. All individuals were genotyped using 11 microsatellite markers from the Maize Genetics and Genomics Database. A Bayesian clustering approach via Monte Carlo Markov chains was performed to examine the genetic differentiation (Fst values) among different clusters. The results indicate the existence of three distinct and strongly differentiated genetic groups (K = 3). Moreover, the Fst values (calculated among clusters) were significantly different according to Bayesian credible...
Tipo: Journal article
Palavras-chave: Genetic differentiation; Microsatellite markers; Bayesian clustering; Monte Carlo Markov chains.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392013000200003