Consulta: subjectFacets:"SNP"
Registros recuperados: 42
Data/hora: 09/06/2024 09:49:21

Factor 9 de crecimiento y diferenciación asociado al índice de prolificidad en la oveja pelibuey.
Provedor de dados: 32
Autores: Pérez Ruíz, Elizabeth.
El Factor 9 de Crecimiento y Diferenciación (GDF9) es miembro de la súperfamilia β de factores de crecimiento (TGFβ) y su expresión en el ovocito es esencial para el desarrollo y crecimiento folicular. Diferentes mutaciones en el gen GDF9 han sido asociadas con el incremento de tasa de ovulación y/o prolificidad en algunas razas de ovejas, por lo que el objetivo de este estudio fue la búsqueda de polimorfismos de una sola base (SNPs) en el gen GDF9, así como la asociación entre polimorfismos del gen GDF9 y el índice de prolificidad en ovejas de la raza Pelibuey. Se tomaron muestras sanguíneas de la vena yugular de 16 ovejas y fueron conservadas en papel FTA® (Whatman Mini Card). El exón dos del gen fue amplificado mediante la técnica de Polimerase Chain...
Palavras-chave: Análisis de secuencias; GDF9; Mutación; Polimorfismo; PCR; SNP; Sequence analysis; Mutation; Polymorphism; Maestría; Ganadería.
Ano: 2012
URL: http://hdl.handle.net/10521/1712

Desarrollo y validación de marcadores moleculares para identificar genotipos de maíz de calidad proteínica.
Provedor de dados: 32
Autores: Hernández Rodríguez, Martha.
El maíz (Zea mays L.) de calidad proteínica (QPM) es nutricionalmente superior en comparación con el maíz normal, porque produce semillas con casi el doble de los aminoácidos (aa) lisina (Lys) y triptófano (Trp), esenciales para la síntesis endógena de la vitamina B niacina. Actualmente, la conversión de una línea de maíz normal a una QPM, se acelera mediante la selección asistida por marcadores moleculares (MAS) y métodos de detección química. En este estudio se reporta el desarrollo y validación de marcadores basados en polimorfismos de un solo nucleótido (SNPs) para los loci opaque-2 (o2) y Ask-2, involucrados en la genética de los QPM, así como la interacción de ambos loci para el contenido de 11 aa en el endospermo de dos poblaciones F2 de maíz,...
Palavras-chave: Zea mays; Opaque-2; Ask-2; MAS; QPM; SNP; SSCP; Genética; Doctorado.
Ano: 2013
URL: http://hdl.handle.net/10521/2319

A C1069G SNP of the MC4R gene and its association with economic traits in Korean native cattle (brown, brindle, and black)
Provedor de dados: 69
Autores: Lee,Youngsub; Park,Sairom; Kim,Hun; Lee,Seung Kyu; Kim,Jin Woo; Lee,Hak Kyo; Jeong,Dong Kee; Lee,Sung Jin.
Background: The melanocortin-4 receptor gene (MC4R) plays an important role in regulating food intake and body weight in mammals. In the present study, we identified the MC4R gene in native Korean brown, brindle, and black cattle by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), and investigated its association with economic traits. A total of 413 cattle from the three breeds were tested for backfat thickness, carcass weight, longissimus muscle area, and marbling score, and statistical data were analyzed using the SAS program. Results: The C allele had the highest frequency in brown and brindle cattle, and the G allele frequency was highest in black cattle. The C1069G SNP was significantly (p &lt; 0.05) associated with only...
Tipo: Journal article
Palavras-chave: Economic trait; Marbling scores; MC4R gene; PCR-RFLP; SNP.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500014

Polymorphisms of the bovine chemokine receptor-like 1 gene and their associations with meat quality traits in Qinchuan cattle (Bos taurus)
Provedor de dados: 69
Autores: Zhan,Xiaoli; Jiang,Bijie; Ma,Xianghui; Fu,Changzhen; Zan,Linsen.
Background: We investigated the polymorphisms of the bovine chemokine receptor-like 1(CMKLR1) gene. The coding region of CMKLR1 was screened in Qinchuan cattle by PCR-RFLP technology. Results: In this study, we discovered two single nucleotide polymorphisms (SNPs) (264G &gt; C and 762C &gt; T) in the coding region of the CMKLR1 gene. Hence, we described the BmgT120l and Pdm1 PCR-RFLP methods for detecting the 64G &gt; C and 762C &gt; T mutations, respectively. PCR-RFLP and sequencing were used to analyze the two loci of CMKLR1 gene in 324 individuals, which were randomly selected from breeding populations. Furthermore, meat quality traits in another 80 Qinchuan individuals were analyzed by the comparison between the genotypes and their phenotypic data....
Tipo: Journal article
Palavras-chave: CMKLR1 gene; Meat quality traits; PCR-RFLP; Qinchuan cattle; SNP.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000100003

Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle
Provedor de dados: 69
Autores: Fu,Changzhen; Wang,Hong; Li,Yaokun; Wang,Jiali; Cheng,Gong; Wang,Hongbao; Yang,Wucai; Zan,Linsen.
Background CDIPT (CDP-diacylglycerol-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In recent years, PtdIns has been considered to play an essential role in energy metabolism, fatty acid metabolic pathway and intracellular signal transduction in eukaryotic cells. Results In this study, the results of real-time polymerase chain reaction (PCR) showed that the expression of CDIPT gene was remarkably different in diverse tissues. We also detected the polymorphism of bovine CDIPT gene and analyzed its association with body measurement and meat quality...
Tipo: Journal article
Palavras-chave: Body measurement traits; Gene expression; Meat quality traits; PCR-RFLP; SNP.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400004

Meat tenderness genetic polymorphisms occurrence and distribution in five Zebu breeds in Mexico
Provedor de dados: 69
Autores: Parra-Bracamonte,Gaspar Manuel; Martínez-González,Juan C; Sifuentes-Rincón,Ana M; Moreno-Medina,Victor R; Ortega-Rivas,Eligio.
Background The Zebu cattle are represented by a diverse group of breeds in México. Traditionally these breeds have been associated with the tough beef characteristic. Validated genetic markers have the potential to be included in marker-assisted selection and management programs in order to improve traits such as beef tenderness. The incidence and distribution of Calpain and Calpastatin polymorphisms strongly associated with beef tenderness were estimated in registered cattle of five Zebu breeds in Mexico. Results A low and in some cases null frequency of favorable C allele of CAPN316 was determined in all breeds. Conversely, a more equilibrated frequency in CAPN4751 and CAST loci was observed. Conclusions Although the relatively low occurrence of...
Tipo: Journal article
Palavras-chave: Calpain; Calpastatin; Frequencies; Genotype; SNP.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500007

One-step, codominant detection of imidazolinone resistance mutations in weedy rice (Oryza sativa L.)
Provedor de dados: 69
Autores: Rosas,Juan E; Bonnecarrère,Victoria; Pérez de Vida,Fernando.
Background Weedy rice (Oryza sativa L.) is a noxious form of cultivated rice (O. sativa L.) associated with intensive rice production and dry seeding. A cost-efficient strategy to control this weed is the Clearfield rice production system, which combines imidazolinone herbicides with mutant imidazolinone-resistant rice varieties. However, imidazolinone resistance mutations can be introgressed in weedy rice populations by natural outcrossing, reducing the life span of the Clearfield technology. Timely and accurate detection of imidazolinone resistance mutations in weedy rice may contribute to avoiding the multiplication and dispersion of resistant weeds and to protect the Clearfield system. Thus, highly sensitive and specific methods with high throughput...
Tipo: Journal article
Palavras-chave: Clearfield rice; DNA-based resistance diagnosis; Herbicide resistance; KASP; Red rice; SNP.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200007

The cuttable C-related genotype and allele for the E-cadherin 3’-UTR Pml I polymorphism are associated with higher susceptibility to endometriosis
Provedor de dados: 74
Autores: Hsieh,Yao-Yuan; Chang,Chi-Chen; Tsai,Fuu-Jen; Hsu,Chin-Moo; Lin,Cheng-Chieh; Tsai,Chang-Hai.
Epithelial cadherin (E-cadherin; CDH1) may influence pericellular proteolysis and intracellular signal transduction, which plays an essential part of tumor invasion. In our study we investigated the correlation between CDH1 gene polymorphism and endometriosis in two groups of pre-menopausal Taiwanese women, group 1 (n = 150) consisting of women with severe stage IV endometriosis and group 2 (n = 159) of women with no endometriosis. The polymerase chain reaction (PCR) was used to identify the cuttable (C) and uncuttable (T) polymorphism of the CDH1-Pml I gene (rs1801026) located on the 3’-untranslated region (3’-UTR) of chromosome 16 and compare the genotypes and allelic frequencies of this gene in both groups. We found that the genotype and allele...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Endometriosis; E-cadherin; Polymorphism; SNP.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500003

Aromatase gene and its effects on growth, reproductive and maternal ability traits in a multibreed sheep population from Brazil
Provedor de dados: 74
Autores: Lôbo,Ana Maria Bezerra Oliveira; Lôbo,Raimundo Nonato Braga; Paiva,Samuel Rezende.
We determined the polymorphism C242T of the aromatase gene (Cyp19) and its allelic frequency, as well as the effect of the variants on productive and reproductive traits in 71 purebred Santa Inês sheep, 13 purebred Brazilian Somali sheep, nine purebred Poll Dorset sheep, and 18 crossbred 1/2 Dorper sheep. The animals were genotyped using the PCR-RFLP technique. The influence of the animal's genotype on its performance or on the performance of its lambs was analyzed by the least square method. Another factor assessed was the importance of the animal's genotype in analysis models for quantitative breeding value estimates, and whether there were differences among the averages of breeding values of animals with different genotypes for this gene. In the sample...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Body weight; Lambing interval; PCR-RFLP; Polymorphism; SNP; Litter weight.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300010

Dyslexia risk variant rs600753 is linked with dyslexia-specific differential allelic expression of DYX1C1
Provedor de dados: 74
Autores: Müller,Bent; Boltze,Johannes; Czepezauer,Ivonne; Hesse,Volker; Wilcke,Arndt; Kirsten,Holger.
Abstract An increasing number of genetic variants involved in dyslexia development were discovered during the last years, yet little is known about the molecular functional mechanisms of these SNPs. In this study we investigated whether dyslexia candidate SNPs have a direct, disease-specific effect on local expression levels of the assumed target gene by using a differential allelic expression assay. In total, 12 SNPs previously associated with dyslexia and related phenotypes were suitable for analysis. Transcripts corresponding to four SNPs were sufficiently expressed in 28 cell lines originating from controls and a family affected by dyslexia. We observed a significant effect of rs600753 on expression levels of DYX1C1 in forward and reverse sequencing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dyslexia; SNP; EQTL; Differential allelic expression.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100041

Mitochondrial DNA single nucleotide polymorphism associated with weight estimated breeding values in Nelore cattle (Bos indicus)
Provedor de dados: 74
Autores: Biase,Fernando Henrique; Meirelles,Flávio Vieira; Gunski,Ricardo; Vozzi,Pedro Alejandro; Bezerra,Luiz A.F.; Vila,Reginaldo A.; Rosa,Artur J.M.; Lôbo,Raysildo B.; Martelli,Lúcia.
We sampled 119 Nelore cattle (Bos indicus), 69 harboring B. indicus mtDNA plus 50 carrying Bos taurus mtDNA, to estimate the frequencies of putative mtDNA single nucleotide polymorphisms (SNPs) and investigate their association with Nelore weight and scrotal circumference estimated breeding values (EBVs). The PCR restriction fragment length polymorphism (PCR-RFLP) method was used to detect polymorphisms in the mitochondrial asparagine, cysteine, glycine, leucine and proline transporter RNA (tRNA) genes (tRNAasn, tRNAcys, tRNAgly, tRNAleu and tRNApro). The 50 cattle carrying B. taurus mtDNA were monomorphic for all the tRNA gene SNPs analyzed, suggesting that they are specific to mtDNA from B. indicus cattle. No tRNAcys or tRNAgly polymorphisms were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Mitochondria; MtDNA; SNP; Weight.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600005

Chromosomal localization and molecular marker development of the lipopolysaccharide and beta-1,3-glucan binding protein gene in the Zhikong scallop Chlamys farreri (Jones et Preston) (Pectinoida, Pectinidae)
Provedor de dados: 74
Autores: Huan,Pin; Zhang,Xiaojun; Li,Fuhua; Zhang,Yang; Zhao,Cui; Xiang,Jianhai.
Zhikong scallop Chlamys farreri (Jones et Preston) is an economically important species in China. Understanding its immune system would be of great help in controlling diseases. In the present study, an important immunity-related gene, the Lipopolysaccharide and Beta-1,3-glucan Binding Protein (LGBP) gene, was located on C. farreri chromosomes by mapping several lgbp-containing BAC clones through fluorescence in situ hybridization (FISH). Through the localization of various BAC clones, it was shown that only one locus of this gene existed in the genome of C. farreri, and that this was located on the long arm of a pair of homologous chromosomes. Molecular markers, consisting of eight single nucleotide polymorphism (SNPs) markers and one insertion-deletion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlamys farreri; LGBP; Fluorescence in situ hybridization (FISH); Indel; SNP.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100008

Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP
Provedor de dados: 74
Autores: Raut,Ashwin A.; Kumar,Anil; Kala,Sheo N.; Chhokar,Vinod; Rana,Neeraj; Beniwal,Vikas; Jaglan,Sundeep; Samuchiwal,Sachin K.; Singh,Jitender K.; Mishra,Anamika.
Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and DNA sequencing to examine polymorphism in the region spanning exon 7 to exon 9 of the DGAT1 gene in Murrah and Pandharpuri buffaloes. Three alleles (A, B and C) and four novel single-nucleotide polymorphisms were identified in the buffalo DGAT1 gene. The frequencies of the alleles differed between the two buffalo breeds, with allele C being present in Murrah but not in Pandharpuri buffalo. The allele variation detected in this work may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buffalo; DGAT1; PCR-SSCP; Polymorphism; SNP.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400011

Genome-wide association study for birth, weaning and yearling weight in Colombian Brahman cattle
Provedor de dados: 74
Autores: Martínez,Rodrigo; Bejarano,Diego; Gómez,Yolanda; Dasoneville,Romain; Jiménez,Ariel; Even,Gael; Sölkner,Johann; Mészáros,Gabor.
Abstract Genotypic and phenotypic data of 1,562 animals were analyzed to find genomic regions that potentially influence the birth weight (BW), weaning weight at seven months of age (WW) and yearling weight (YW) of Colombian Brahman cattle, with genotyping conducted using Illumina Bead chip array with 74,669 SNPs. A Single Step Genomic BLUP (ssGBLP), approach was used to estimate the proportion of variance explained by each marker. Multiple regions scattered across the genome were found to influence weights at different ages, also dependent on the trait component (direct or maternal). The most interesting regions were connected to previously identified QTLs and genes, such as ADAMTSL3, CAPN2, CAPN2, FABP6, ZEB2 influencing growth and weight traits. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bos indicus; SNP; QTL; GWAS; Body weight.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300453

ESTs and putative line-specific (broiler and layer) SNPs identified in genes expressed in Gallus gallus pituitary and hypothalamus
Provedor de dados: 74
Autores: Cassoli,Clarissa Sanches da Silva; Jorge,Erika Cirstina; Patrício,Mateus; Alves,Helena Javiel; Almeida,Erik Amazonas de; Ledur,Mônica Corrêa; Coutinho,Luiz Lehmann.
Brazilian poultry industry has reached a high level of development in both meat and egg production as a result of constant technological modernization. Further improvements can be achieved through genomics, but before this can be accomplished, a better understanding of gene expression profiles and nucleotide polymorphisms is necessary. Since animal physiology is directly or indirectly controlled by the pituitary and hypothalamus, the aim of the present work was to identify and analyze genes expressed in these tissues in chicken lines with different growth potential. Two pituitary and hypothalamus cDNA libraries from 21 day broiler (TT) and layer (CC) chickens lines were constructed and allowed identification of 3,074 unique sequences and 77 single...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pituitary; Hypothalamus; EST; SNP; Chicken.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600008

Validação de marcadores moleculares associados à pungência em pimenta
Provedor de dados: 75
Autores: Pereira,Ítalo S; Barreto,Fernanda Z; Balsalobre,Thiago WA; Sala,Fernando C; Costa,Cyro P; Carneiro,Monalisa S.
A pungência em frutos de pimenta do gênero Capsicum, devido à presença de capsaicinóides, é uma das características mais atrativas e muito valorizadas pela culinária mundial. Neste trabalho, objetivou-se validar e avaliar a eficiência de predição dos marcadores moleculares pun1¹, pun1³ e o SNP identificado pelo método tetra-primer ARMS-PCR para a determinação de pungência em acessos de Capsicum spp. Trinta e seis acessos do Banco de Germoplasma da Universidade Federal de São Carlos foram avaliados através de análises sensoriais (em frutos maduros) e moleculares para pungência (na fase de plântula através da extração do DNA em folhas). Os marcadores SNP ARMS-PCR, pun1¹ e pun1³ foram usados na avaliação molecular. Os resultados mostram a eficiência desses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum spp.; SNP; Melhoramento genético; Seleção.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362015000200009

Diversidade nucleotídica e utilização de SNPs para o mapeamento de genes candidatos em Eucalyptus spp.
Provedor de dados: 14
Autores: NEVES, L. G.; FARIA, D. A. de; PAPPAS JUNIOR, G. J.; PASQUALI, G.; GRATTAPAGLIA.
bitstream/CENARGEN-2009-09/31528/3/cot180_1008.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Diversidade nucleotídica; SNP; Mapeamento genético.; Eucalyptus spp.; Eucalipto.
Ano: 2008
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/191157

Single Nucleotide polymorphisms and their relationship to codon usage bias in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Sauvage, Christopher; Bierne, N; Lapegue, Sylvie; Boudry, Pierre.
DNA sequence polymorphism and codon usage bias were investigated in a set of 41 nuclear loci in the Pacific oyster Crassostrea gigas. Our results revealed a very high level of DNA polymorphism in oysters, in the order of magnitude of the highest levels reported in animals to date. A total of 290 single nucleotide polymorphisms (SNPs) were detected, 76 of which being localised in exons and 214 in non-coding regions. Average density of SNPs was estimated to be one SNP every 60 bp in coding regions and one every 40 bp in non-coding regions. Non-synonymous substitutions contributed substantially to the polymorphism observed in coding regions. The non-synonymous to silent diversity ratio was 0.16 on average, which is fairly higher to the ratio reported in other...
Tipo: Text
Palavras-chave: Crassostrea gigas; Codon bias; Genetic diversity; SNP.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3417.pdf

Transcriptome based SNP discovery and validation for parentage assignment in hatchery progeny of the European abalone Haliotis tuberculata
Provedor de dados: 5
Autores: Harney, Ewan; Lachambre, Sebastien; Roussel, Sabine; Huchette, Sylvain; Enez, Florian; Morvezen, Romain; Haffray, Pierrick; Boudry, Pierre.
Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by...
Tipo: Text
Palavras-chave: Abalone; Parentage assignment; Pedigree; Selective breeding; Transcriptome; SNP.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00429/54061/55396.pdf

APIS: An Auto‐Adaptive Parentage Inference Software that tolerates missing parents
Provedor de dados: 5
Autores: Griot, Ronan; Allal, Francois; Brard‐fudulea, S; Morvezen, R; Haffray, P; Phocas, F; Vandeputte, Marc.
In the context of parentage assignment using genomic markers, key issues are genotyping errors and an absence of parent genotypes because of sampling, traceability or genotyping problems. Most likelihood‐based parentage assignment software programs require a priori estimates of genotyping errors and the proportion of missing parents to set up meaningful assignment decision rules. We present here the R package APIS, which can assign offspring to their parents without any prior information other than the offspring and parental genotypes, and a user‐defined, acceptable error rate among assigned offspring. Assignment decision rules use the distributions of average Mendelian transmission probabilities, which enable estimates of the proportion of offspring with...
Tipo: Text
Palavras-chave: Microsatellites; Missing parents; Parentage assignment; Pedigree; SNP.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00587/69866/67767.pdf

Structure et diversité génétique des populations de raie bouclée en Méditerranée
Provedor de dados: 5
Autores: Lecuyer, Romain.
Chondrichtyes are threatened by several disturbances among which is the significant development of fisheries during the 20th century. The Mediterranean sea has long been fished and keeps being intensely so by several nations that seek cost-effectiveness or food security above all. Such a background does not ease data recording and sharing for research purposes. This study mainly aims at assessing the population structure and status of thornback ray so as to avoid its deterioration due to bottom-trawl fisheries pressure, hardly reversible even with a sudden sound management. To do so, an extensive panel of nuclear genetic markers has been developed to analyze underlying structure along with differenciation and genetic diversity of Mediterranean populations....
Tipo: Text
Palavras-chave: Méditerranée; Structure de populations; Différenciation; Diversité génétique; SNP; Raja clavata; Mediterranean sea; Population structure; Differenciation; Genetic diversity; SNP; Raja clavata.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00667/77899/80091.pdf

Rol de la calpastatina en la variabilidad de la terneza de la carne bovina
Provedor de dados: 46
Autores: Motter,MM; Corva,P; Krause,M; Perez Cenci,M; Soria,L.
La calpastatina es una enzima inhibidora de las calpaínas, principales enzimas proteolíticas del músculo esquelético. Está codificada por el gen CAST, a partir del cual se pueden expresar cuatro isoformas proteicas diferentes debido a la existencia de cuatro promotores distintos. Este gen ha sido estudiado como candidato para explicar diferencias de origen genético en la terneza de la carne. En bovinos se han descripto varios polimorfismos del tipo SNP en distintas regiones del gen. Algunos de ellos han sido incluidos en test comerciales por su asociación significativa con la variabilidad en terneza. Recientemente, la investigación se ha focalizado en establecer qué promotores están activos en músculo esquelético y si los mismos presentan polimorfismos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calpastatina; Gen CAST; Bovinos; Terneza; SNP.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332009000100003

Antecedentes y nuevos aportes en el estudio del Cromosoma Y en poblaciones humanas sudamericanas
Provedor de dados: 46
Autores: Bailliet,Graciela; Ramallo,Virginia; Muzzio,Marina; Santos,María R; Motti,Josefina MB; Bianchi,Néstor O; Bravi,Claudio M.
Los avances en el conocimiento del genoma humano han llegado también a una situación comprensiva en relación a la región específica del cromosoma Y humano. En los últimos años se han reconocido al menos 600 polimorfismos bialélicos (SNP), a partir de los cuales se ha construido una sólida filogenia y se ha normalizado la nomenclatura. En el presente trabajo se han estudiado 940 muestras de varones no relacionados de 10 poblaciones aborígenes de Argentina, Chile y Paraguay; y 12 poblaciones urbanas de Argentina. Se han analizado 9 marcadores bialélicos para reconocer los clados mayores de la filogenia. Fue posible distinguir los haplogrupos Q* y Q3 autóctonos, y los haplogrupos AB, DE, F, K, P y R alóctonos. El Fst fue de 0, 17, mostrando un alto nivel de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Linajes paternos; Haplogrupos; SNP; Ancestralidad genética; Poblaciones humanas.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332011000100003

Alternative genotyping method for the single nucleotide polymorphism A2959G (AF159246) of the bovine CAST gene
Provedor de dados: 86
Autores: Curi,Rogério Abdallah; Chardulo,Luis Artur Loyola; Silveira,Antônio Carlos; Oliveira,Henrique Nunes de.
The objective of this work was to genotype the single nucleotide polymorphism (SNP) A2959G (AF159246) of bovine CAST gene by PCR-RFLP technique, and to report its use for the first time. For this, 147 Bos indicus and Bos taurus x Bos indicus animals were genotyped. The accuracy of the method was confirmed through the direct sequencing of PCR products of nine individuals. The lowest frequency of the meat tenderness favorable allele (A) in Bos indicus was confirmed. The use of PCR-RFLP for the genotyping of the bovine CAST gene SNP was shown to be robust and inexpensive, which will greatly facilitate its analysis by laboratories with basic structure.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calpastatin gene; Meat texture; PCR-RFLP; SNP.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500014

Polymorphism in Litopenaeus vannamei genes and cross-species amplification in other shrimp species
Provedor de dados: 86
Autores: Santos,Camilla Alves; Farias,Flavio; Teixeira,Ana Karina; Rocha,João Luís; Guerrelhas,Ana Carolina; Freitas,Patrícia Domingues de.
Abstract: The objective of this work was to assess polymorphisms within Litopenaeus vannamei candidate genes related to growth performance, and a possible association between weight genotypes and phenotypes in two shrimp closed lines. Three EST-SSR (serine-arginine, 60S ribosomal, and troponin) and one EST-SNP (crustacyanin) were selected and evaluated in L. vannamei and seven other marine shrimp species, besides two freshwater species. Genotypes had no effect on total body weight. Although no association was observed in the sampled population, these markers showed amplification for most of the evaluated species and, therefore, may be useful in further genetic studies on shrimp species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candidate genes; Crustacyanin; Microsatellite; SNP; Weight-association; 60S ribosomal.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100121

Endonuclease com incompatibilidade heteroduplex para detectar mutação e variações genéticas de inibidores da tripsina em soja
Provedor de dados: 86
Autores: Petrović,Gordana; Nikolić,Zorica; Đor&amp;#273;ević,Vuk; Župunski,Vesna; Jovičić,Dušica; Ignjatov,Maja; Milošević,Dragana.
O objetivo deste trabalho foi avaliar a variação genética do inibidor de tripsina em variedades cultivadas (Glycine max) e silvestres (Glycine soja) de soja. Foram avaliadas as variações genéticas do inibidor de tripsina Kunitz, representado pela proteína 21-kDa (KTI), e do inibidor de tripsina-quimotripsina Bowman-Birk (BBI), em variedades de soja cultivadas (G. max) e selvagens (G. soja). Ensaios de clivagem foram feitos com endonuclease de incompatibilidade heteroduplex, para a detectar mutações no gene de KTI, com uma única nuclease específica de cadeia simples, obtida a partir de extractos de aipo (CEL I). As variedades de soja estudadas apresentaram baixo nível de variação genética em KTI e BBI. A análise por PCR -RFLP dividiu o BBI-A em A1 e A2 e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Glycine soja; Factores antinutritionais; Inibidores de protease; SNP.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2014000200102

THE SINGLE NUCLEOTIDE POLYMORPHISMS OF MYOSTATIN GENE AND THEIR ASSOCIATIONS WITH GROWTH AND CARCASS TRAITS IN DAHENG BROILER
Provedor de dados: 89
Autores: Zhang,XX; Ran,JS; Lian,T; Li,ZQ; Yang,CW; Jiang,XS; Du,HR; Cui,ZF; Liu,YP.
ABSTRACT Myostatin (MSTN) is a negative regulator of skeletal muscle growth. In order to investigate whether there is a correlation between MSTN polymorphisms and chicken production performance, in this study, single nucleotide polymorphisms (SNPs) in MSTN gene were examined across 180 Daheng broilers by direct sequencing of PCR product, and the correlations between the genotype and body weight at the age of 1-10 weeks and carcass traits at the age of 73 day were analyzed. Five SNPs (rs313622770, rs313744840, rs316247861, rs314431084, rs317126751) of MSTN gene were identified across Daheng broiler samples, and four haplotypes were reconstructed based on the five SNPs. Results of association analysis showed that four (rs313622770, rs313744840, rs316247861...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Association analysis; Chicken; Production performance; MSTN; SNP.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300319

Alternative Transcription of Peroxisome Proliferator-Activated Receptor Gamma in the Liver Is Associated with Fatness of Chickens
Provedor de dados: 89
Autores: Parada,R; Malewski,T; Jaszczak,K; Kawka,M.
ABSTRACT The expression of four transcription variant of peroxisome proliferator-activated receptor gamma gene (PPARG) (XM_015292931.1; XM_015292932.1; XM_015292933.1 and NM_001001460.1) in the liver of broilers was measured and its correlation with abdominal fat weight and relative abdominal fat content was investigated. The study was conducted with 92 slow-growing crossbred chickens (Cobb males x indigenous Green-legged Partridge female chickens) divided into “fat” and “lean” groups, according to their abdominal fat yield. The NM_001001460.1 transcriptwas upregulated with ratio of means 4.26 (p≤0.01) in the “fat” group in relation to the “lean” group. Expression of this transcript was highly correlated with relative abdominal fat content (0.71, p≤0.01)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fat deposition; PPARG; Transcription factor; SNP; Slow-growing chickens.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300447

Association Analysis Between Polymorphism of Gonadotrophin Releasing Hormone Genes and Growth Traits of Quail (Coturnix Coturnix)
Provedor de dados: 89
Autores: Bai,J; Dong,ZH; Lei,Y; Yang,YB; Jia,XP; Li,JY.
ABSTRACT This study investigated SNP mutation sites of Gonadotrophin releasing hormone (GnRH) gene in China yellow quail, Beijing white quail and Korean quail through PCR amplification and DNA sequencing technologies. Moreover, polymorphism of GnRH gene and its association with growth traits of quail were analyzed, aiming to get molecular markers associated to growth traits of quail, which could provide references for breeding of new quail species. According to research results, a total of 14 SNP mutation sites of GnRH were detected in China yellow quail, Beijing white quail and Korean quail, which were C71T, C108T, C168T, C178T, A184G, C206T, A209C, C215T, A252G, A279T, C281T, C293G, C339T and C458T. Except that only 2 genotypes were detected for A209C...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gonadotrophin releasing hormone (GnRH) gene; DNA sequencing; SNP; Polymorphism; Body weight.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100312

Study on the polymorphism of POU1F1 gene in sheep
Provedor de dados: 96
Autores: Bai,Jun Yan; Wang,Xu; Yang,You Bing; Zhang,Xiao Hui; Pang,You Zhi; Li,Hong Wei.
ABSTRACT In this study, POU1F1 gene polymorphism was detected in five sheep populations (large-tailed Han, small-tailed Han, Yuxi fat-tailed, Lanzhou large-tailed, and Mongolian sheep), using DNA pooling and sequencing, to provide theoretical basis for the breeding of excellent sheep varieties. Three single-nucleotide polymorphism (SNP) loci of POU1F1 gene were detected in five sheep populations, namely C355T (C/T), C71G (C/G), and C330G (C/G). C and T frequencies of C355T were 0.67/0.33, 0.81/0.19, 0.67/0.33, 1.00/0.00, and 0.93/0.07, respectively, in large-tailed Han, small-tailed Han, Yuxi fat-tailed, Mongolian, and Lanzhou large-tailed sheep. C of C355T locus was the dominant allele in five sheep populations. C and G allele frequencies of C330G locus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA pooling; Phylogenetic tree; Sheep; SNP.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016001000604

Ant colony algorithm for analysis of gene interaction in high-dimensional association data
Provedor de dados: 96
Autores: Rekaya,Romdhane; Robbins,Kelly.
In recent years there has been much focus on the use of single nucleotide polymorphism (SNP) fine genome mapping to identify causative mutations for traits of interest; however, many studies focus only on the marginal effects of markers, ignoring potential gene interactions. Simulation studies have show that this approach may not be powerful enough to detect important loci when gene interactions are present. While several studies have examined potential gene interaction, they tend to focus on a small number of SNP markers. Given the prohibitive computation cost of modeling interactions in studies involving a large number SNP, methods need to be develop that can account for potential gene interactions in a computationally efficient manner. This study adopts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genome; Simulation; SNP.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001300011

Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle
Provedor de dados: 96
Autores: Carvalho,Thiago Dutra de; Siqueira,Fabiane; Torres Júnior,Roberto Augusto de Almeida; Medeiros,Sérgio Raposo de; Feijó,Gelson Luís Dias; Souza Junior,Maury Dorta de; Blecha,Isabella Maiumi Zaidan; Soares,Cleber Oliveira.
The objective of the present study was to estimate the allelic and genotypic frequencies of the polymorphisms E2FB (AY138588.1: c.305C&gt; T), located in the leptin gene (LEP), and TG5 (X05380.1:g.-422C&gt;T), located in the thyroglobulin gene (TG), and evaluate the association of these polymorphisms in crossbred cattle of seven distinct genetic groups with the following traits: slaughter weight (SW), hot carcass weight (HCW), hot carcass yield (HCY), carcass fat thickness (CFT), ribeye area (REA), marbling (MARM) and shear force (SF). The animals were genotyped using the PCR-RFLP (Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism) technique, using 201 products obtained from F1 Caracu × Nellore, Angus × Nellore and Valdostana × Nellore...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fat thickness; Marker-assisted selection; Meat tenderness; Shear force; SNP.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000004

Influence of temperament-related genes on live weight traits of Charolais cows
Provedor de dados: 96
Autores: Garza-Brenner,Estela; Sifuentes-Rincón,Ana María; Rodríguez-Almeida,Felipe Alonso; Randel,Ronald D.; Parra-Bracamonte,Gaspar Manuel; Arellano-Vera,Williams.
Abstract We investigated whether a panel of molecular markers previously associated with temperament has an effect on live growth traits. Phenotypic data from 412 Charolais cows categorized according to their age in adult and young cows were used to determine Pearson's correlations between birth (BW), weaning (WW), and yearling live (YW) weight and temperament traits (measured as exit velocity [EV] and temperament score [TS]). For association analysis, selective genotyping of a group of 80 cows identified as the most docile and temperamental were genotyped with a 151-SNP (single-nucleotide polymorphisms) panel of molecular markers previously associated with temperament. Significant Pearson's correlations between birth weight and weaning weight and the two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Behavior; Body weight; Candidate genes; SNP; Temperament.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982020000100401

Análise de diversidade genética do gene da osteopontina em bovinos da raça girolando
Provedor de dados: 96
Autores: Mello,Fernanda de; Guimarães,Marta Fonseca Martins; Cobuci,Jaime Araújo; Silva,Marcos Vinícius Gualberto Barbosa da; Braccini Neto,José; Paiva,Daisyléa de Souza.
Objetivou-se obter os índices de diversidade genética para o SNP (single nucleotide polymorphism) do íntron 4 do gene da osteopontina (OPN) para 434 animais (87 touros e 347 vacas) participantes do Teste de Progênie da raça girolando no Brasil. Para a amplificação, foram utilizados primers descritos para a raça holandesa, e a diferenciação dos alelos C/T desse SNP foi obtida por meio da técnica de PCR-RFLP. As frequências genotípicas TT (52,53%), CT (38,71%) e CC (8,76%) e as frequências alélicas de T (71,9%) e C (28,1%) indicam que a população encontra-se em Equilíbrio de Hardy-Weinberg (EHW). Apesar de o loco do gene OPN estar em EHW, a frequência superior do alelo T do SNP nesses animais pode sugerir uma tendência de fixação do alelo T na raça. Não foi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovinos leiteiros; Polimorfismo; Produção de leite; SNP; SPP1.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011001100013

Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle
Provedor de dados: 96
Autores: Blecha,Isabella Maiumi Zaidan; Sousa,Isadora Inácio; Ferreira,Anna Beatriz Robottom; Feijó,Gelson Luis Dias; Torres Júnior,Roberto Augusto de Almeida; Siqueira,Fabiane.
ABSTRACT The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A&gt;G (CAST/DdeI) and AF248054.2:g.6545C&gt;T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal breeding; Marker-assisted selection; PCR-RFLP; SNP; Tenderness.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100311

Seleção genômica ampla para curvas de crescimento
Provedor de dados: 45
Autores: Silva,F.F.; Rocha,G.S.; Resende,M.D.V.; Guimarães,S.E.F.; Peternelli,L.A.; Duarte,D.A.S.; Azevedo,C.
Foi proposta uma metodologia para avaliação genética de curvas de crescimento considerando-se informações de marcadores SNPs (Single Nucleotide Polymorphisms). Em um primeiro passo foram ajustados modelos de crescimento não lineares (logístico) aos dados de peso-idade de cada animal, e em um segundo passo as estimativas dos parâmetros de tais modelos foram consideradas como fenótipos em um modelo de regressão (LASSO Bayesiano - BL) cujas covariáveis foram os genótipos dos marcadores SNPs. Este enfoque possibilitou estimar os valores genéticos genômicos (GBV) para peso em qualquer tempo da trajetória de crescimento, refletindo na confecção de curvas de crescimento genômicas, as quais permitiram a identificação de grupos de indivíduos geneticamente...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SNP; LASSO bayesiano; Dados longitudinais.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500033

Characterization of gene polymorphisms related to immune system physiology in Mangalarga horses
Provedor de dados: 45
Autores: Prioli,R.A.; Curi,R.A.; Chardulo,L.A.; Gomes,V.N.; Prioli,S.M.A.P.; Mota,M.D.S.
The objectives of this study were to standardize a PCR-RFLP genotyping method for the AY_731081:g.1900T&gt;C SNP of the equine CD14 gene, and to characterize this SNP and two other polymorphisms (AY_005808: c.1530A&gt;G of the TLR4 gene and AX_463789: g.133T&gt;C of the Cε gene) in Mangalarga horses, in order to contribute to future studies investigating the association between DNA markers and traits related to immune system physiology in this breed. A total of 151 Mangalarga horses of both sexes and variable ages, representative of the population of São Paulo State, were used. PCR-RFLP was found to be adequate for genotyping of the AY_731081: g.1900T&gt;C SNP of the equine CD14 gene. However, this polymorphism is probably not present in Mangalarga horses,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse; CD14; Cε; SNP; TLR4.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000500030

Association of PRKAA1 gene polymorphisms with chronic hepatitis B virus infection in Chinese Han population
Provedor de dados: 55
Autores: Yuan,Jun; Zhang,Yan; Yan,Fu-tang; Zheng,Xiao.
ABSTRACT  Objective: Studies have indicated that AMPK play critical roles in the regulation of innate immunity and inflammatory responses. However, the role of the polymorphisms of PRKAA1 gene in immune-response to infectious organisms remains unknown. To evaluate the potential role of PRKAA1/AMPKα1 in the immune-response to HBV, we conducted this case-control study.  Methods: We recruited 276 patients (145 men and 131 women; average age, 51.6 years) with chronic HBV infection (CHB) and 303 healthy controls (166 men and 137 women; average age, 54.2 years). All the subjects were unrelated individuals of Chinese Han Population. Three SNPs of PRKAA1gene were tested.  Results: Rs1002424 polymorphism showed significant difference in the allele frequencies, but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HBV; PRKAA1 gene; SNP; Association study.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600564

Single-nucleotide polymorphism analysis of GH, GHR, and IGF-1 genes in minipigs
Provedor de dados: 56
Autores: Tian,Y.G.; Yue,M.; Gu,Y.; Gu,W.W.; Wang,Y.J.
Tibetan (TB) and Bama (BM) miniature pigs are two popular pig breeds that are used as experimental animals in China due to their small body size. Here, we analyzed single-nucleotide polymorphisms (SNPs) in gene fragments that are closely related to growth traits [growth hormone (GH), growth hormone receptor (GHR), and insulin-like growth factor (IGF)-1)] in these pig breeds and a large white (LW) control pig breed. On the basis of the analysis of 100 BMs, 108 TBs, and 50 LWs, the polymorphic distribution levels of GH, GHR, and IGF-1 were significantly different among these three pig breeds. According to correlation analyses between SNPs and five growth traits - body weight (BW), body length (BL), withers height (WH), chest circumference (CC), and abdomen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Minipigs; SNP; GH; GHR; IGF-1.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900753

Genetic association of SNPs in the FTO gene and predisposition to obesity in Malaysian Malays
Provedor de dados: 56
Autores: Apalasamy,Y.D.; Ming,M.F.; Rampal,S.; Bulgiba,A.; Mohamed,Z.
The common variants in the fat mass- and obesity-associated (FTO) gene have been previously found to be associated with obesity in various adult populations. The objective of the present study was to investigate whether the single nucleotide polymorphisms (SNPs) and linkage disequilibrium (LD) blocks in various regions of the FTO gene are associated with predisposition to obesity in Malaysian Malays. Thirty-one FTO SNPs were genotyped in 587 (158 obese and 429 non-obese) Malaysian Malay subjects. Obesity traits and lipid profiles were measured and single-marker association testing, LD testing, and haplotype association analysis were performed. LD analysis of the FTO SNPs revealed the presence of 57 regions with complete LD (D’ = 1.0). In addition, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FTO gene; SNP; Obesity; Linkage disequilibrium; Haplotypes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200003

Heterozygous HTRA1 missense mutation in CADASIL-like family disease
Provedor de dados: 56
Autores: Wu,Xiaowei; Li,Changxin; Mao,Jinming; Li,Ling; Liu,Yan; Hou,Yao.
The aim of this study was to find related pathogenic genes in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy in (CADASIL)-like patients. The direct sequencing and high-throughput multiplex polymerase chain reaction (PCR) was performed to screen for related genes. The clinical and imaging data of a CADASIL-like patient (the pro-band) and his family members were collected. At first, the known hereditary cerebral vascular genes of the pro-band were screened with direct sequencing to find candidate gene mutations. High-throughput multiplex PCR was then used to analyze the single nucleotide polymorphism of the candidate gene in the family members. The results showed that there was missense mutation of the high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HTRA1; CADASIL-like; SNP; Cerebral small vessel disease.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500602

The optimal number of partial least squares components in genomic selection for pork pH
Provedor de dados: 65
Autores: Silveira,Fernanda Gomes da; Duarte,Darlene Ana Souza; Chaves,Lucas Monteiro; Silva,Fabyano Fonseca e; Filho,Ivan Carvalho; Duarte,Marcio de Souza; Lopes,Paulo Sávio; Guimarães,Simone Eliza Facioni.
ABSTRACT: The main application of genomic selection (GS) is the early identification of genetically superior animals for traits difficult-to-measure or lately evaluated, such as meat pH (measured after slaughter). Because the number of markers in GS is generally larger than the number of genotyped animals and these markers are highly correlated owing to linkage disequilibrium, statistical methods based on dimensionality reduction have been proposed. Among them, the partial least squares (PLS) technique stands out, because of its simplicity and high predictive accuracy. However, choosing the optimal number of components remains a relevant issue for PLS applications. Thus, we applied PLS (and principal component and traditional multiple regression)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SNP; Genomic prediction; Meat quality.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100652