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Caracterización morfológica y molecular de aislamientos de Monilinia fructicola obtenidos de frutos de durazno [Prunus persica (L.) Batsch] en México y pruebas de agresividad sobre durazno, pera y manzana.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Robles Yerena, Leticia.
Existen tres especies de Monilinia reportadas como causantes de la pudrición café de los frutos de hueso: M. fructigena y M. laxa de origen Europeo y M. fructicola de origen Americano. En este trabajo 50 cultivos monospóricos de Monilinia spp. de frutos de durazno provenientes de los Estados de Aguascalientes, Chihuahua, Michoacán, Morelos y Zacatecas, México colectados durante el 2008-2009. La caracterización molecular se hizo mediante PCR e ITS y se confirmó a Monilinia fructicola con base en la banda de 500 pb y la amplificación de la región interna ITS-1 e ITS-2. Para la caracterización morfológica PDA y agar-durazno (AD2) fueron seleccionados. Las colonias fueron color gris marrón, de crecimiento continuo uniforme y los márgenes de las colonias fueron...
Palavras-chave: Monilinia fructigena; Pudrición café; Postcosecha; Medios de cultivo; PCR; Brown rot; Postharvest; Media cultures; Maestría; Fitopatología.
Ano: 2010
URL: http://hdl.handle.net/10521/84

Detección e incidencia del fitoplasma Maize Bushy Stunt y su relación con el rendimiento en maíz en el Estado de Veracruz, México
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Alcántara Mendoza, Susana.
Una nueva enfermedad del maíz apareció en el estado de Veracruz, México durante 2003-2004. Síntomas de amarillamiento, enrojecimiento y posterior quemado de hojas, proliferación de mazorcas, jilotes raquíticos, falta de fecundación y granos vanos fueron parte del síndrome de la enfermedad. El fitoplasma Maize Bushy Stunt (MBS) se identifico por PCR se detectó en plantas con los síntomas antes descritos, en los municipios de Tlalixcoyan, Cosoleacaque y Paso de Ovejas en 2006 y 2007. Los genotipos CP-562, 30F83, 30F92, Orca, 30F96, Nutria y Asgrow 7573 resultaron positivos a la presencia del MBS, mientras que en 3086, 30F97 y CP-560 no se detectó la presencia del MBS en una plantación experimental en plantas sintomáticas en Tlalixcoyan, Ver. en 2006 y 2007....
Tipo: Tesis
Palavras-chave: Maize Bushy Stunt; PCR; Incidencia; Resistencia genética; Genoma y  homología; Maestría; Fitopatología; Maize Bushy Stunt; Incidence; Genetic resistance; Genome and homology.
Ano: 2009
URL: http://hdl.handle.net/10521/1574

Búsqueda de portainjertos de aguacate tolerantes resistentes a Phytophthora cinnamomi Rands
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Castañeda González, Elba Lidia.
México se considera el centro de origen del aguacate y mantiene diversidad genética  en  materiales criollos.  En este estudio, se realizaron colectas de semilla para  detectar  tolerancia o resistencia a Phytophthora cinnamomi Rands, principal patógeno de la raíz  del aguacate. El material se colectó en los estados de Querétaro, Puebla y Estado de  México, seleccionando árboles que no mostraban síntomas (árboles “escape”) aún  cuando los huertos están infestados con el patógeno. Además, se incluyeron en la  investigación semillas de 22 accesiones del Banco de Germoplasma (BG) de la  Fundación Salvador Sánchez Colín CICTAMEX S.C. y planta clonal del portainjerto  Duke-7 resistente a P. cinnamomi y utilizado como testigo. Se inocularon 5,200  plántulas con...
Tipo: Tesis
Palavras-chave: Persea americana Mill.; Portainjertos; PCR Persea americana Mill.; Rootstock; PCR.
Ano: 2012
URL: http://hdl.handle.net/10521/1071

Etiología del variegado del chile (Capsicum annuum L.) en Yurécuaro, Michoacán.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Hernández Deheza, María Guadalupe.
En Yurécuaro, Michoacán, en el ciclo de cultivo verano otoño 2009 y 2011 se encontraron en cultivos de chile síntomas de variegado, nervaduras cloróticas, deformación de la lámina foliar y fruto, albinismo y escaldadura, síndrome al cual se le denominó “variegado del chile”. A simple vista, esta serie de síntomas indicaban que el problema era causado por virus fitopatógenos; sin embargo, en ensayos previos se detectó a Candidatus Liberibacter solanacearum, por lo que el objetivo del presente trabajo fue determinar si esta bacteria es el agente causal del variegado del chile. Mediante transmisión por injerto, transmisión con el vector Bactericera cockerelli y reacción en cadena de la polimerasa con el par de iniciadores OA2/CLi.po.F y OI2, se confirmo que...
Palavras-chave: Candidatus Liberibacter; PCR; Secuenciación; Sequencing; Entomología y Acarología; Doctorado.
Ano: 2013
URL: http://hdl.handle.net/10521/1844

Variantes alélicas del gen de la Kappa-Caseína en ganado criollo lechero tropical.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Álvarez Cepeda, Amado Alberto.
Las variantes A y B del gen de la κ-caseína influyen en la producción y composición de la leche y sus derivados. La variante B está relacionada con un mayor rendimiento de la cuajada. El objetivo de este estudio fue determinar las frecuencias genotípicas y alélicas de las variantes A y B del gen de la κ-caseína en la población de hembras Criollo Lechero Tropical y su relación con la composición química de la leche. Se  recolectaron muestras de sangre de la vena coccígea de las hembras utilizando tubos Vacutainer de 5 ml adicionados con el anticoagulante EDTA. La extracción de ADN y la amplificación y digestión del gen, se realizó bajo condiciones de laboratorio. Se utilizó la técnica de Reacción en Cadena de la Polimerasa (PCR) para amplificar el gen de la...
Palavras-chave: Criollo Lechero Tropical; PCR; RFLP; K-caseína; Tropical Milking Creole; K-casein; Ganadería; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2259

Determinación de la dieta del jaguar (Panthera onca) y puma (Puma concolor) en el municipio de Tamasopo, San Luis Potosí
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Rueda Zozaya, Rocío del Pilar.
En México se encuentran los felinos más grandes de América, el puma (Puma concolor) y el jaguar (Panthera onca). Debido al incremento en las fronteras agropecuarias, el jaguar y el puma se encuentran en peligro de extinción en varias áreas de su distribución. Aunada a su situación crítica, su comportamiento elusivo y críptico dificulta aún más la accesibilidad para realizar investigación acerca de su etología, hábitos alimenticios y estimación de parámetros poblacionales. Por ello, se ha tenido la necesidad de recurrir a otros  métodos de investigación no invasivos que eviten el manejo directo, uno de estos métodos es la recolección de excretas para identificar los componentes de su dieta, con esta información, se puede evaluar si realmente estos felinos...
Palavras-chave: Panthera onca; Puma concolor; Dieta; PCR; Diet; Ganadería; Fauna silvestre; Maestría.
Ano: 2010
URL: http://hdl.handle.net/10521/76

Factor 9 de crecimiento y diferenciación asociado al índice de prolificidad en la oveja pelibuey.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Pérez Ruíz, Elizabeth.
El Factor 9 de Crecimiento y Diferenciación (GDF9) es miembro de la súperfamilia β de factores de crecimiento (TGFβ) y su expresión en el ovocito es esencial para el desarrollo y crecimiento folicular. Diferentes mutaciones en el gen GDF9 han sido asociadas con el incremento de tasa de ovulación y/o prolificidad en algunas razas de ovejas, por lo que el objetivo de este estudio fue la búsqueda de polimorfismos de una sola base (SNPs) en el gen GDF9, así como la asociación entre polimorfismos del gen GDF9 y el índice de prolificidad en ovejas de la raza Pelibuey. Se tomaron muestras sanguíneas de la vena yugular de 16 ovejas y fueron conservadas en papel FTA® (Whatman Mini Card). El exón dos del gen fue amplificado mediante la técnica de Polimerase Chain...
Palavras-chave: Análisis de secuencias; GDF9; Mutación; Polimorfismo; PCR; SNP; Sequence analysis; Mutation; Polymorphism; Maestría; Ganadería.
Ano: 2012
URL: http://hdl.handle.net/10521/1712

Variación morfométrica y genética de Diaphorina citri (Hemiptera: Liviidae) en rutáceas de Veracruz, México.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Cuellar Sánchez, Valeria Isabel.
Diaphorina citri es el vector más importante del Huanglongbing, la enfermedad más destructiva de los cítricos en el mundo. El objetivo de este estudio fue detectar la presencia de variantes morfométricas y genéticas, a través del análisis de secuencias de nucleótidos mediante RAPDs y el gen mitocondrial mtCOI para determinar si existe correspondencia entre variantes de individuos de D. citri Kuwayama (Hemiptera: Liviidae), colectados en rutáceas de Veracruz, México. Se encontraron variaciones morfométricas en el ancho, largo y venación de las alas y de los procesos genales de los especímenes estudiados. Los análisis genéticos por medio de RAPDs y secuencias obtenidas con el gen mtCOI confirman la existencia de cuatro haplotipos (H1-H4) de D. citri...
Palavras-chave: Psílido asiático de los cítricos; Morfometría; RAPDs; COI; PCR; Asiatic citrus psyllid; Morphometrics; Entomología y Acarología; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2346

Detección e incidencia del fitoplasma Maize Bushy Stunt y su relación con el rendimiento en maíz en el estado de Veracruz, México
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Alcántara Mendoza, Susana.
Una nueva enfermedad del maíz apareció en el estado de Veracruz, México durante 2003-2004. Síntomas de amarillamiento, enrojecimiento y posterior quemado de hojas, proliferación de mazorcas, jilotes raquíticos, falta de fecundación y granos vanos fueron parte del síndrome de la enfermedad. El fitoplasma Maize Bushy Stunt (MBS) se identifico por PCR se detectó en plantas con los síntomas antes descritos, en los municipios de Tlalixcoyan, Cosoleacaque y Paso de Ovejas en 2006 y 2007. Los genotipos CP-562, 30F83, 30F92, Orca, 30F96, Nutria y Asgrow 7573 resultaron positivos a la presencia del MBS, mientras que en 3086, 30F97 y CP-560 no se detectó la presencia del MBS en una plantación experimental en plantas sintomáticas en Tlalixcoyan, Ver. en 2006 y 2007....
Palavras-chave: Maize Bushy Stunt; PCR; Incidencia; Resistencia genética; Genoma; Homología; Incidence; Genetic resistance; Genome; Homology; Maestría en Ciencias; Postgrado en Fitosanidad; Fitopatología.
Ano: 2009
URL: http://hdl.handle.net/10521/53

Antracnosis del fruto de litchi (Litchi chinensis Soon.) : etiología y control in vitro.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Martínez Bolaños, Misael.
El litchi (Litchi chinensis Soon) es un fruto subtropical. El cultivar Brewster se explota comercialmente en la región del Papaloapan y el cv. Mauritius en los Bajos Mixes, Oaxaca, México. El manchado del pericarpio es uno de los problemas principales en pre y postcosecha del fruto en ambos cultivares. No se han encontrado registros de su causa o importancia en México, por ello los objetivos de esta investigación fueron: determinar la etiología del manchado café del pericarpio e iniciar la exploración del  control in vitro de su agente causal. La antracnosis o manchado del pericarpio del litchi se caracterizó externa e internamente por lesiones circulares de color café, con márgenes bien definidos en el pericarpio, pero sin afectar el arilo o la semilla....
Palavras-chave: Brewster; Mauritus; Pathogenicity; Incidence; PCR; Patogenicidad; Incidencia; Antracnosis; Benomil; Procloraz; Hidróxido de cobre; Doctorado; Fitopatología.
Ano: 2012
URL: http://hdl.handle.net/10521/1701

Determinación de la dieta del jaguar (Panthera onca) y puma (Puma concolor) en el municipio de Tamasopo, San Luis Potosí
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Rueda Zozaya, Rocío del Pilar.
En México se encuentran los felinos más grandes de América, el puma (Puma concolor) y el jaguar (Panthera onca). Debido al incremento en las fronteras agropecuarias, el jaguar y el puma se encuentran en peligro de extinción en varias áreas de su distribución. Aunada a su situación crítica, su comportamiento elusivo y críptico dificulta aún más la accesibilidad para realizar investigación acerca de su etología, hábitos alimenticios y estimación de parámetros poblacionales. Por ello, se ha tenido la necesidad de recurrir a otros  métodos de investigación no invasivos que eviten el manejo directo, uno de estos métodos es la recolección de excretas para identificar los componentes de su dieta, con esta información, se puede evaluar si realmente estos felinos...
Palavras-chave: Panthera onca; Puma concolor; Dieta; PCR; Diet; Ganadería; Fauna silvestre; Maestría.
Ano: 2010
URL: http://hdl.handle.net/10521/76

Búsqueda de portainjertos de aguacate tolerantes resistentes a Phytophthora cinnamomi Rands
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Castañeda González, Elba Lidia.
México se considera el centro de origen del aguacate y mantiene diversidad genética  en  materiales criollos.  En este estudio, se realizaron colectas de semilla para  detectar  tolerancia o resistencia a Phytophthora cinnamomi Rands, principal patógeno de la raíz  del aguacate. El material se colectó en los estados de Querétaro, Puebla y Estado de  México, seleccionando árboles que no mostraban síntomas (árboles “escape”) aún  cuando los huertos están infestados con el patógeno. Además, se incluyeron en la  investigación semillas de 22 accesiones del Banco de Germoplasma (BG) de la  Fundación Salvador Sánchez Colín CICTAMEX S.C. y planta clonal del portainjerto  Duke-7 resistente a P. cinnamomi y utilizado como testigo. Se inocularon 5,200  plántulas con...
Tipo: Tesis
Palavras-chave: Persea americana Mill.; Portainjertos; PCR; Doctorado; Fitopatología; Rootstock.
Ano: 2009
URL: http://hdl.handle.net/10521/1334

Identificación filogenética de Candidatus Liberibacter solanacearum y su efecto en la calidad fisiológica de la semilla de chile jalapeño.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Olivares Mercado, Perla Xochitl.
En 1993 se reportó por primera vez en México a Candidatus Liberibacter solanacearum, como el agente causal de la pigmentación morada en las hojas, reducción de tamaño de la planta y necrosis interna de los tubérculos de la papa; posteriormente se detectó este bacterium en los cultivos de tomate, chile y berenjena. Sin embargo, la distribución de las plantas sintomáticas en el campo no tenía un patrón definido hasta que en 2009 se observó la totalidad de plantas atípicas de chile jalapeño cv Ixtapa en dos campos de los estados de Nayarit y Jalisco. Las plantas tuvieron una reducción de tamaño en un 30%, hojas con coloración verde pálido y deformación de los frutos en un 100%. Por tal razón la presente investigación tuvo como objetivos identificar el agente...
Palavras-chave: Chile jalapeño; Candidatus Liberibacter solanacearum; PCR; Clonación; Microscopia electrónica de barrido; Calidad de la semilla; Jalapeño pepper; Cloning; Electronic microscopy scanning; Seed quality; Producción de Semillas; Maestría.
Ano: 2013
URL: http://hdl.handle.net/10521/1956

Detección e incidencia del fitoplasma Maize Bushy Stunt y su relación con el rendimiento en maíz en el estado de Veracruz, México
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Alcántara Mendoza, Susana.
Una nueva enfermedad del maíz apareció en el estado de Veracruz, México durante 2003-2004. Síntomas de amarillamiento, enrojecimiento y posterior quemado de hojas, proliferación de mazorcas, jilotes raquíticos, falta de fecundación y granos vanos fueron parte del síndrome de la enfermedad. El fitoplasma Maize Bushy Stunt (MBS) se identifico por PCR se detectó en plantas con los síntomas antes descritos, en los municipios de Tlalixcoyan, Cosoleacaque y Paso de Ovejas en 2006 y 2007. Los genotipos CP-562, 30F83, 30F92, Orca, 30F96, Nutria y Asgrow 7573 resultaron positivos a la presencia del MBS, mientras que en 3086, 30F97 y CP-560 no se detectó la presencia del MBS en una plantación experimental en plantas sintomáticas en Tlalixcoyan, Ver. en 2006 y 2007....
Palavras-chave: Maize Bushy Stunt; PCR; Incidencia; Resistencia genética; Genoma; Homología; Incidence; Genetic resistance; Genome; Homology; Maestría; Postgrado en Fitosanidad; Fitopatología.
Ano: 2009
URL: http://hdl.handle.net/10521/53

Caracterización morfológica y molecular de aislamientos de Monilinia fructicola obtenidos de frutos de durazno [Prunus persica (L.) Batsch] en México y pruebas de agresividad sobre durazno, pera y manzana.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Robles Yerena, Leticia.
Existen tres especies de Monilinia reportadas como causantes de la pudrición café de los frutos de hueso: M. fructigena y M. laxa de origen Europeo y M. fructicola de origen Americano. En este trabajo 50 cultivos monospóricos de Monilinia spp. de frutos de durazno provenientes de los Estados de Aguascalientes, Chihuahua, Michoacán, Morelos y Zacatecas, México colectados durante el 2008-2009. La caracterización molecular se hizo mediante PCR e ITS y se confirmó a Monilinia fructicola con base en la banda de 500 pb y la amplificación de la región interna ITS-1 e ITS-2. Para la caracterización morfológica PDA y agar-durazno (AD2) fueron seleccionados. Las colonias fueron color gris marrón, de crecimiento continuo uniforme y los márgenes de las colonias fueron...
Palavras-chave: Monilinia fructigena; Pudrición café; Postcosecha; Medios de cultivo; PCR; Brown rot; Postharvest; Media cultures; Maestría; Fitopatología.
Ano: 2010
URL: http://hdl.handle.net/10521/84

Estandarización y validación de la técnica de PCR para el diagnóstico de Salmonella typhimurium en carne de cerdo, res y pollo.
Provedor de dados: Depósito Institucional Abierto de Documentos Digitales del Colegio de Postgraduados (COLPOS DIGITAL )
Autores: Rosas Espejel, Monzerrat.
El estudio tuvo como objetivo estandarizar y validar la técnica de Reacción en Cadena de la Polimerasa (PCR) para la detección de Salmonella typhimurium (St) en carne de cerdo, res y pollo, optimizando el tiempo y la sensibilidad con relación al método microbiológico; además se realizó el diagnóstico en estos tipos de carnes para evaluar su calidad microbiológica en la Ciudad de Texcoco. Un total de 60 muestras de carne (20 de cerdo, 20 de res y 20 de pollo) de 250 g fueron recolectadas en puntos de venta de la Ciudad de Texcoco. Las muestras recolectadas procedían de carnicerías, tianguis y supermercados. Se evaluó la sensibilidad y especificidad de la PCR para detectar St; asimismo, se validó esta técnica en relación al método microbiológico con base al...
Palavras-chave: Salmonella typhimurium; PCR; Carne; Validación; Meat; Validation; Ganadería; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2388

A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages
Provedor de dados: Electronic Journal of Biotechnology
Autores: Kundu,Avijit; Sarkar,Debabrata; Bhattacharjee,Amit; Topdar,Niladri; Sinha,Mohit Kumar; Mahapatra,Bikash Sinha.
A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-NaCl DNA extraction followed by ethanol-based removal of remnant mucilages from the DNA pellet, this simple miniprep consistently and reproducibly recovers high amounts of DNA with good spectral qualities at A260/A280 and A260/A230. The purified DNA is efficiently digested by restriction endonucleases, and is suitable for PCR amplification of nuclear microsatellites with expected allele sizes.
Tipo: Journal article
Palavras-chave: Corchorus; DNA extraction; Microsatellite; Mucilage; PCR; Restriction digestion.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100010

LaNe RAGE: a new tool for genomic DNA flanking sequence determination
Provedor de dados: Electronic Journal of Biotechnology
Autores: Park,Daniel J.
The determination of genomic DNA sequence flanking a known region is often problematic. Existing technologies depend on multiple, efficient enzyme-catalysed preparative processing steps and/or rely on relatively inefficient ‘one-sided' PCR mechanisms. I demonstrate the application of a simple ‘two-sided' PCR-based approach, lariat-dependent nested PCR for rapid amplification of genomic DNA ends (LaNe RAGE), applied to the mouse GAPDH and PGK1 gene flanking sequences. This demonstration offers great promise in applications such as genome walking, transposon mutagenesis mapping and DNA fingerprinting
Tipo: Journal article
Palavras-chave: Cloning; DNA fingerprinting; Genomic DNA walking; PCR; Sequencing; Transposon mutagenesis.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200012

Bt protein rhizosecreted from transgenic maize does not accumulate in soil
Provedor de dados: Electronic Journal of Biotechnology
Autores: Margarit,Ezequiel; Reggiardo,Martín I; Permingeat,Hugo R.
The persistence of CryIAb protein rhizosecreted in soil is important in the assessment of its environmental risk. Here we report that CryIAb protein from transgenic maize does not accumulate at high levels in soils. Levels of CryIAb protein rhizosecreted by three maize transgenic events (BT11, MON810 and 176) were studied in hydroponic cultures and found only in the MON810 and BT11 events but not in event 176 or control plants. Under field conditions, the cryIAb gene and a basal level of CryIAb protein was detected in soils from plots cultivated with transgenic and non-transgenic maize, possibly from Bacillus thuringiensis present in the soils.
Tipo: Journal article
Palavras-chave: CryIAb gene; CryIAb protein; ELISA; Genetically modified organisms; PCR; Zea mays (L.).
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200004

Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
Provedor de dados: Electronic Journal of Biotechnology
Autores: Wang,Chuan Tang; Yu,Shan Lin; Zhang,Shu Wei; Wang,Xiu Zhen; Tang,Yue Yi; Zhang,Jian Cheng; Chen,Dian Xu.
A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.
Tipo: Journal article
Palavras-chave: Identification; NIRS; PCR; Peanut; True hybrid.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500018

Cotton genetic diversity study by AFLP markers
Provedor de dados: Electronic Journal of Biotechnology
Autores: Murtaza,Naveed.
Amplified fragmentlength polymorphism (AFLP) markers have been used to ascertain the intensity of inherent diversity and relatedness in cotton (Gossypium spp.) plants. The effectiveness of this method to distinguish inter and intra specific difference in cotton could be handy in cultivar recognition and in marker assisted parental selection tool for plant breeders. Twenty cotton cultivars belonging to Gossypium hirsutum L., and G. arborium L. from the Pakistan and US origin were used for AFLP based genetic diversity estimates. The objective of this study was to assess the level of genetic variation among some cotton cultivars belonging to the old and new world species of cotton. Four EcoRI-MseI primer-pair combinations were used forthe AFLP analysis. The...
Tipo: Journal article
Palavras-chave: AFLP; Cluster analysis; Cotton; Genetic diversity; PCR.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400013

A simple and efficient method for extraction of Taq DNA polymerase
Provedor de dados: Electronic Journal of Biotechnology
Autores: Chen,Sique; Zheng,Xiujuan; Cao,Hongrui; Jiang,Linghui; Liu,Fangqian; Sun,Xinli.
Background Thermostable DNA polymerase (Taq Pol ?) from Thermus aquaticus has been widely used in PCR, which was usually extracted with Pluthero's method. The method used ammonium sulfate to precipitate the enzyme, and it saved effort and money but not time. Moreover, we found that 30-40% activity of Taq Pol I was lost at the ammonium sulfate precipitation step, and the product contained a small amount of DNA. Results We provided a novel, simplified and low-cost method to purify the Taq Pol ? after overproduction of the enzyme in Escherichia coli, which used ethanol instead of ammonium sulfate to precipitate the enzyme. The precipitate can be directly dissolved in the storage buffer without dialysis. In addition, DNA and RNA contamination was removed with...
Tipo: Journal article
Palavras-chave: Ethanol precipitation; PCR; Purification; Taq DNA polymerase.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500005

PCR-based sensitive detection of the edible fungus Boletus edulis from rDNA ITS sequences
Provedor de dados: Electronic Journal of Biotechnology
Autores: Lian,Bin; Zang,Jin-ping; Hou,Wei-guo; Yuan,Sheng; Smith,Donald L.
Identification of commercially important fungi, such as the valuable edible fungus Boletus edulis can be difficult considering visual or metabolic approaches. Based on phylogenetic analysis of the rDNA ITS sequence, a pair of specific primers was designed for differentiating B. edulis from other mushrooms by PCR. PCR was performed with total DNA as a template at an annealing temperature between 56-60ºC. Positive amplicons were obtained from B. edulis with all DNA templates from fruit bodies and cultured mycelium, but not from other fungal species at an annealing temperature of 60ºC. The result indicated that B. edulis could be clearly distinguished from other fungi by PCR, and there were no misidentifications under the reaction conditions used. The primers...
Tipo: Journal article
Palavras-chave: Boletus edulis; Detection; Edible fungi; Internal transcribed spacer; PCR; Specific primers.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300011

Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction
Provedor de dados: Electronic Journal of Biotechnology
Autores: Yu,Shu Tao; Wang,Chuan Tang; Yu,Shan Lin; Wang,Xiu Zhen; Tang,Yue Yi; Chen,Dian Xu; Zhang,Jian Cheng.
An efficient DNA extraction method was developed for peanut seed, where only 3-5 mg cotyledonary tissue was enough for more than 50 PCR reactions with a reaction volume of 15 &amp;#956;l. Both low copy number and high copy number DNA sequences were successfully amplified. Processing one seed sample only took about half an hour. Sampling had no significant effects on germination and development. The DNA extraction method makes it possible to identify transformants and conduct molecular marker studies prior to sowing, and thus may greatly hasten research progress.
Tipo: Journal article
Palavras-chave: Cotyledonary tissue; DNA extraction; Groundnut; PCR; Peanut.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400012

A rapid and cheap protocol for preparation of PCR templates in peanut
Provedor de dados: Electronic Journal of Biotechnology
Autores: Wang,Chuan Tang; Wang,Xiu Zhen; Tang,Yue Yi; Zhang,Jian Cheng; Yu,Shan Lin; Xu,Jian Zhi; Bao,Zhen Min.
This paper describes a simple, low cost and reliable DNA template preparation protocol for polymerase chain reaction (PCR) using immature leaves from peanut seeds or leaves from field-grown plants. The technique may find wide utility in studies involving PCR-based molecular markers, rapid screening for transformants and gene cloning.
Tipo: Journal article
Palavras-chave: DNA extraction; Groundnut; PCR; Peanut.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200009

The lift pool method for isolation of cDNA clones from lambda phage libraries
Provedor de dados: Electronic Journal of Biotechnology
Autores: LeBlanc-Straceski,Janine; Sobrado,Pablo; Betz,Sharon; Zerfas,Julie; Morgan,Karen.
A PCR based strategy was developed to screen a Xenopus oocyte &amp;lambda;gt10 cDNA library. The PCR-based lift pool (LP) method follows the same two tiered strategy as conventional screening of phage libraries by filter hybridization. Two rounds of plating, one at high density to detect the clone, and one at low density to purify the clone to homogeneity, are performed. In the first round, lysates from high density plates, termed plate pools (PP), serve as template for PCR. In the second round, phage particles adhering to plaque lifts of low density plates are washed off nitrocellulose filters to create LPs, which are used as template for PCR. The integrity of the plaques on the low-density plates is preserved. Once a positive LP has been identified,...
Tipo: Journal article
Palavras-chave: CDNA; Lambda; Non-radioactive; PCR; Plaque; Screening.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400012

Discrimination of Shark species by simple PCR of 5S rDNA repeats
Provedor de dados: Genetics and Molecular Biology
Autores: Pinhal,Danillo; Gadig,Otto BF; Wasko,Adriane P; Oliveira,Claudio; Ron,Ernesto; Foresti,Fausto; Martins,Cesar.
Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chondrichthyes; PCR; Species identification; 5S rDNA; Sharks.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200033

Distribution and conservation of the transposable element gypsy in drosophilid species
Provedor de dados: Genetics and Molecular Biology
Autores: Herédia,Fabiana; Loreto,Elgion Lúcio S.; Valente,Vera Lúcia S.
In an attempt to understand the dynamics of transposable elements (T'S) in the genome of host species, we investigated the distribution, representativeness and conservation of DNA sequences homologous to the Drosophila melanogaster gypsy retrotransposon in 42 drosophilid species. Our results extended the knowledge about the wide distribution of gypsy in the genus Drosophila, including several Neotropical species not previously studied. The gypsy-like sequences showed high divergence compared to the D. melanogaster gypsy element. Furthermore, the conservation of the restriction sites between gypsy sequences from phylogenetically unrelated species pointed to a more complex evolutionary picture, which includes the possibility of the horizontal transfer events...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila; Retrotransposon; Gypsy; Southern blot; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100023

Isolation and sequencing of seven Sox genes from the lacertid lizard Eremias breuchleyi
Provedor de dados: Genetics and Molecular Biology
Autores: Jie,Li; Zheng,Ping-Ping; Song,Jiao-Lian; Rui,Jin-Long; Nie,Liu-Wang.
The Sox family of genes shares a high sequence similarity with the HMG box region of the human Y chromosomal gene, SRY. We used highly degenerate primers to clone and sequence seven Eremias breuchleyi Sox genes (EbSox2, EbSox3, EbSox4, EbSox11, EbSox12, EbSox14 and EbSox21). A database search for the cloned sequences revealed the following percentage identity with the homologous human SOX genes: EbSox2 = 96%, EbSox3 = 88%, EbSox4 = 94%, EbSox11 = 99%, EbSox12 = 96%, EbSox14 = 98%, EbSox21 = 97%. Cluster analysis indicates that they seem to belong to group B and group C of Sox gene family, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eremias breuchleyi; PCR; Sequence analysis; Sox genes; SSCP.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300031

Medium-sized deletion in the BRCA1 gene: limitations of Sanger sequencing and MLPA analyses
Provedor de dados: Genetics and Molecular Biology
Autores: Herman,Sandra; Varga,Dominic; Deissler,Heidrun L.; Kreienberg,Rolf; Deissler,Helmut.
We describe a family with a history of breast and ovarian cancer in which MLPA analysis of the BRCA1 gene pointed to a deletion including a part of exon 11. Further characterization confirmed a loss of 374 bp in a region completely covered by conventional sequencing which had not revealed the deletion. Because this alteration was only detected serendipitously with an MLPA probe, we calculated the probabilities of detecting medium-sized deletions in large exons by methods including initial PCR amplification. This showed that a considerable fraction of medium-sized deletions are undetectable by currently used standard methods of mutation analyses. We conclude that long, widely overlapping amplicons should be used to minimize the risk of missing medium-sized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutation analysis; DNA sequencing; PCR; Hereditary breast cancer; Model calculations.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100007

Use of the TSPY gene for sexing cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Lemos,Daniela Cristina; Rios,Álvaro Fabrício Lopes; Caetano,Lisandra Cristina; Lôbo,Raysildo Barbosa; Vila,Reginaldo Aparecido; Martelli,Lúcia; Takeuchi,Paula Lumy; Ramos,Ester Silveira.
The Y-encoded, testis-specific protein (TSPY) is a Y-specific gene. The copy numbers of TSPY range from 20 to 60 in men and up to 200 in bulls. In this study, we examined the possibility of using the TSPY gene to sex cattle. DNA from blood samples of 100 Nelore cattle (50 males and 50 females) from the Nelore Cattle Breeding Program (PMGRN) was screened for TSPY by PCR using TSPY-specific primers. The assay was highly specific since all male samples were TSPY-positive and all female samples were negative. Positive results were also obtained at low DNA concentrations (less than 1 rhog/muL). These results showed that TSPY was a good male-specific marker, the usefulness of which was enhanced by the high copy number of the gene. This is the first report to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Cattle; PCR; Sexing; TSPY.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100020

Parentage testing and effect of misidentification on the estimation of breeding value in Gir cattle
Provedor de dados: Genetics and Molecular Biology
Autores: Baron,Erica E.; Martinez,Mário L.; Verneque,Rui S.; Coutinho,Luiz L.
A correct relationship among sires is essential for an efficient breeding program. Microsatellite markers were used in progeny tests, to assess the paternity of seventy-four probable offspring of nine Gir dairy sires. A 36% misidentification rate was observed; however, these errors had minimal effects on the ranking of the nine bulls with regard to their genetic values. The results suggest that paternity tests should be performed in breeding programs, in order to prevent inappropriate paternities from influencing the genetic value of bulls in the future.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite; Gir; Cattle; Breeding; Parentage; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400006

Association of MYF5 gene allelic variants with production traits in pigs
Provedor de dados: Genetics and Molecular Biology
Autores: Carmo,Fausto Moreira da Silva; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Pires,Aldrin Vieira; Guimarães,Marta Fonseca Martins; Silva,Marcos Vinícius Gualberto Barbosa da; Schierholt,Alex Sandro; Silva,Kleibe de Moraes e; Gomide,Lúcio Alberto de Miranda.
We studied the phenotypic effects of polymorphisms at the MYF5 gene in a divergent F2 swine population and found that one polymorphism was due to an insertion and another to a deletion. The genotypes of 359 F2 animals were obtained and the Normal/Normal (NN) and Normal/Insertion (NI) genotypes analyzed to determine associations with phenotypic data for performance, carcass and meat quality traits. Significant differences were observed (p &lt; 0.05) between NN and NI animals for drip (NN = 3.14 ± 1.56; NI = 3.69 ± 2.78%), cooking (NN = 32.26 ± 2.41; NI = 33.21 ± 2.31%) and total loss (NN = 34.16 ± 2.63 and NI = 34.97 ± 2.08%). The Deletion marker was not statistically tested. The results indicate that the allelic variant Insertion is associated with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myogenesis; DNA sequencing; Pig production; PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300004

Variation in extragenic repetitive DNA sequences in Pseudomonas syringae and potential use of modified REP primers in the identification of closely related isolates
Provedor de dados: Genetics and Molecular Biology
Autores: Çepni,Elif; Gürel,Filiz.
In this study, Pseudomonas syringe pathovars isolated from olive, tomato and bean were identified by species-specific PCR and their genetic diversity was assessed by repetitive extragenic palindromic (REP)-PCR. Reverse universal primers for REP-PCR were designed by using the bases of A, T, G or C at the positions of 1, 4 and 11 to identify additional polymorphism in the banding patterns. Binding of the primers to different annealing sites in the genome revealed additional fingerprint patterns in eight isolates of P. savastanoi pv. savastanoi and two isolates of P. syringae pv. tomato. The use of four different bases in the primer sequences did not affect the PCR reproducibility and was very efficient in revealing intra-pathovar diversity, particularly in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial identification; Biodiversity; PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400016

Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers
Provedor de dados: Genetics and Molecular Biology
Autores: Kasuga,Ikuma; Paré,Peter D.; Sandford,Andrew J.
We established a specific genotyping assay for HLA-A*01, which is one of the most frequently found HLA-A alleles in the Caucasian population. This assay uses the polymerase chain reaction (PCR) with allele group-specific primers (ASP). HLA-A*01 group-specific primers were designed for exon 3 of the HLA-A gene, based on the recent HLA-sequence alignment. Both sense and anti-sense primers were designed with completely matched sequences to each specific HLA-A*01 allele, but mismatched by at least 1 nucleotide to all other known class I HLA alleles. By the use of these primers and stringent PCR conditions, we successfully genotyped the HLA-A*01 group alleles and achieved greater accuracy than previous methods.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA-A1; PCR; Allele group-specific primers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200003

Similar interstitial deletions of the KAL-1 gene in two Brazilian families with X-linked Kallmann Syndrome
Provedor de dados: Genetics and Molecular Biology
Autores: Trarbach,Ericka Barbosa; Monlleo,Isabella Lopes; Porciuncula,Carlos Guilherme Gaelzer; Fontes,Marshall Italo Barros; Baptista,Maria Teresa Mathias; Hackel,Christine.
Mutations in the KAL-1 gene localized at Xp22.3 have been shown to be responsible for the X-linked Kallmann syndrome (KS), a disorder characterized by the association of hypogonadotropic hypogonadism and anosmia. In this paper, we describe the investigation of two families with X-linked KS, in which similar interstitial deletions ning exons 5 to 10 of the KAL-1 gene were identified. The presence of interspersed repetitive DNA sequences within the KAL-1 gene might have predisposed to this type of mutation.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: X-linked KS; Intragenic deletions; KAL-1 gene; PCR; Phenotypic variability.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300006

Mitochondrial variability in the D-loop of four equine breeds shown by PCR-SSCP analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Mirol,Patricia M.; Peral García,P.; Dulout,F.N.
A fragment of 466 base pairs from a highly variable peripheral region of the mitochondrial D-loop of horses was amplified and analyzed by single stranded conformational polymorphism (SSCP). Fourteen distinct SSCP variants were detected in 100 horses belonging to four breeds (Arabian, ARB; Thoroughbred, TB; Argentinian Creole, ARC; and Peruvian Paso from Argentina, PPA). Each breed showed four to eight SSCP variants, many of which were shared between two or three of the studied breeds. Arabian horses were the most variable (eigth variants), with three variants unique to the breed. PPA and ARC showed two and one characteristic SSCP variants, respectively, while TB shared all its variants with at least one of the other breeds. An analysis based on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MTDNA; Equine; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100006

PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
Provedor de dados: Genetics and Molecular Biology
Autores: Freitas,Patrícia Domingues de; Galetti Junior,Pedro Manoel.
The genetic variation in two farmed strains (F3-Panama and F17-Venezuela) of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean heterozygosity and the frequency of polymorphic loci were determined for both strains. A dendrogram of genetic similarity was produced by UPGMA clustering. The results for three primers (INS, M13, YN73) revealed different levels of genetic variation within the strains. The higher genetic similarity seen within strain F17 was apparently related to inbreeding, although a bottleneck effect could not be discarded. The low level of genetic variability of this strain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Litopenaeus vannamei; Genetic diversity; Shrimp; VNTR; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012

An analysis of multiplex-PCR in the detection of BCR-ABL transcripts in hematological disorders
Provedor de dados: Genetics and Molecular Biology
Autores: Sastre,Darío A.; Argaraña,Carlos E.; Heller,Viviana B.; Gallo,Mónica; Fernández,Enrique N.; Rodríguez,Cecilia M.
In this work, we describe the advantages of multiplex-PCR in the specific detection of BCR-ABL transcripts in different hematological disorders and its sensitivity compared to nested PCR. Fifty-three patients were studied for the presence of BCR-ABL transcripts: 24 patients with chronic myeloid leukemia (CML), 20 with acute leukemia (AL), and 9 patients with other hematological disorders. A variant rearrangement (b3a3) was found in a single case of CML (4.2%). Four out of the 20 patients with AL (20.0%) (14 adults, 6 children) were bcr-abl(+), and in this group three cases were classified as B-acute lymphoblastic leukemia (B-ALL), and one as acute myeloblastic leukemia (AML). Two of the three patients with B-ALL were positive for b2a2 and the other one for...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Multiplex-PCR; Ph chromosome; BCR-ABL; Chronic myeloid leukemia; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400003

Identificação dos citoplasmas "S", "T" e "N" via PCR em populações de cebola no Vale do São Francisco
Provedor de dados: Horticultura Brasileira
Autores: Santos,Carlos Antonio F; Leite,Daniela L; Costa,Nivaldo D; Oliveira,Valter R; Santos,Ierla Carla N dos; Rodrigues,Marciene A.
A identificação do tipo de citoplasma em cebola foi facilitada com a publicação de primers de DNA específicos para os tipos "S", "T" e "N". O objetivo do presente trabalho foi identificar, por meio de marcadores moleculares, o tipo de citoplasma presente na cultivar de cebola BRS São Francisco e numa população experimental em desenvolvimento na Embrapa Semi-Árido, de forma a orientar o desenvolvimento de híbridos de cebola adaptados à região. Na amostra de 19 plantas da 'BRS Alfa São Francisco' observou-se a amplificação de fragmentos de 180 pb, ausência do fragmento de 414 pb com os primers 5'cob e a amplificação do fragmento de 473 pb dos primers OrfA501, indicando que o citoplasma presente nesta população é o citoplasma T. Foi também identificada a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Macho-esterilidade; PCR; Híbrido.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362008000300003

Seleção do sêmen de reprodutores portadores do vírus da artrite encefalite caprina através da técnica de reação em cadeia da polimerase.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: ANDRIOLI, A.; GOUVEIA, A. M. G.; PINHEIRO, R. R.
A partir de 1976, importações de caprinos de raças exóticas, procedentes de vários países, buscaram a introdução de potencial genético leiteiro, tendo ocorrido também a efetiva introdução de agentes infecciosos no Brasil pela importação de caprinos sem adequados critérios sanitários, com consequente dispersão de doenças infecciosas entre os rebanhos nacionais, dentre elas a CAE - artrite encefalite caprina. Os avanços das técnicas de conservação de sêmen, aliados ao aumento da importância econ0mica da caprinocultura favorecem o seu intercâmbio nacional e internacional. Somando-se a isto, a inseminação artificial possibilita a concepção de um expressivo número de fêmeas, num curta intervalo de tempo, no entanto, pouco se sabe do risco da transição de...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: CAEV; Artrite Encefalite Caprina; Caprine arthritis encephalit virus; Semen diluents.; PCR; Caprino; Reprodução animal; Sêmen; Seleção; Transmissão de doença; Goats; Reproduction; Disease transmission; Infection.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/531309

Identificação de tipo de citoplasma em germoplasma de cebola com base em marcadores moleculares.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: SANTOS, C. A. F.; SÁ, J. F. de; GAMA, R. N. C. de S.; OLIVEIRA, A. E. S. de; OLIVEIRA, V. R.
A identificação de diferentes tipos de citoplasmas associados à macho-esterilidade em cebola foi facilitada com a aplicação de marcadores de DNA em reações de reação em cadeia da polimerase (PCR), auxiliando no desenvolvimento de híbridos. O objetivo deste trabalho foi identificar, por PCR, o tipo de citoplasma presente em acessos brasileiro de cebola, de forma a facilitar o desenvolvimento de híbridos. O DNA de cinco plantas de cada acesso foi extraído de acordo com o protocolo do CTAB 2x. Entre os 30 acessos analisados, observou-se a presença dos três tipos de citoplasma. Doze acessos apresentaram tipo de citoplasma exclusivamente ?N?, sete exclusivamente ?S? e apenas um exclusivamente ?T?. Seis acessos apresentaram mistura entre os tipos de citoplasma...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: CMS; PCR; Macho-esterilidade; Melhoramento genético; Onion; Cebola; Recurso genético; Melhoramento vegetal; Allium Cepa; Citoplasma; Hibridação; Marcador Molecular.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1064469

Análise da expressão gênica pela técnica de PCR quantitativa em tempo real: princípios e fundamentos.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: MORGANTE, C. V.; BLAWID, R.
Neste trabalho são apresentados os conceitos fundamentos da análise da expressão gênica pela técnica de PCR e reúne informações importantes para aqueles que se dedicam ao estudo da genética vegetal. Sumário: Introdução; Rermos básicos utilizados na qPCR; Sistemas para a detecção da fluorescência; Etapas anteriores à reação de qPCR; Parâmetros para a padronização da qPCR; Métodos de quantificação gênica; Referências; Glossário.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: PCR; Metodologia de análise; Biologia molecular; Genética vegetal; Biotecnologia; Biotechnology.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1066218

Rapid and sensitive detection of ostreid Herpesvirus 1 in oyster samples by real-time PCR (Poster)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pepin, Jean-francois; Riou, Antoine; Renault, Tristan.
Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region, which is present in two copies in the OsHV-1 genome and encodes two proteins of unknown function. The assay was applied successfully to rapid diagnosis (100 minutes)...
Tipo: Text
Palavras-chave: Crassostrea gigas; PCR; Oyster; OsHV 1; Detection; Herpes virus 1.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3653.pdf

Detection of ostreid herpesvirus 1 (OsHV-1) DNA in seawater by PCR: influence of water parameters in bioassays
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Vigneron, Vassilia; Solliec, Gaelle; Montanie, Hélène; Renault, Tristan.
Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted...
Tipo: Text
Palavras-chave: Temperature; PCR; Detection; Seawater; Viral DNA; Oyster herpesvirus 1.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2909.pdf

Etude préalable à la diversification d'huîtres creuses en France : isolement reproductif entre Crassostrea gigas et C. angulata
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Balabaud, Karen.
Avec 30 000 tonnes d'huîtres produites chaque année, l'ostréiculture est l'activité principale du bassin de Marennes-Oléron. Caractérisé par une très forte interaction avec les milieux professionnels de la conchyliculture, l'Ifremer se doit avant tout de répondre à leurs questions, en particulier en matière de croissance, de reproduction et surtout en matière de mortalité de l'huître creuse. En effet, l'huître portugaise Crassostrea angulata fut décimée de 1968 à 1972 par un agent pathogène de type iridovirus. Pour faire face à cette disparition des populations d'huîtres japonaises Crassostrea gigas sont alors introduites en France. Mais, les professionnels s'interrogent sur la bonne adaptation de l'huître creuse japonaise à l'environnement français et...
Tipo: Text
Palavras-chave: Génétique; Croisement; Isolement reproductif; Huîtres creuses; Hybrides; Marqueurs moléculaires; PCR; Crassostrea gigas; Crassostrea angulata.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00033/14441/11740.pdf

Toward Detection of Harmful Algae Blooms by in situ Surface Plasmon Resonance Spectroscopy
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Laurent, Sebastien; Colas, Florent; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Lehaitre, Michel; Compere, Chantal.
Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate.
Tipo: Text
Palavras-chave: PCR; Algal detection; In situ; Spectroscopy; Surface plasmon resonance.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6830.pdf

Apoptose et infections à virus de type herpès chez les bivalves marins : détection de séquences codant pour des protéines inhibitrices de l'apoptose
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Didier, Yohann.
Tipo: Text
Palavras-chave: PCR; Apoptosis; Marine bivalve; Herpes like virus.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/rapport-1768.pdf

Contribution à l'étude du système neuroendocrine de la moule Mytilus edulis (Mollusca: Bivalvia)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Kellner-cousin, Kristell.
Dans le cadre de ce travail, l'étude du système neuroendocrine de la moule Mytilus edulis a été envisagée sous plusieurs aspects qui définissent les quatre grandes parties de la thèse.  Dans une première partie est décrit le travail de préparation et de caractérisation d'anticorps monoclonaux (AcMc) spécifiques de ganglions cérébroïdes (GC) de Mytilus edulis. Le développement de techniques de préparation d'AcMc dirigés contre des antigènes non purifiés a en effet permis de produire un ensemble de réactifs de type AcMc dirigés contre différents antigènes issus d'un broyat de GC de moule. La spécificité de ces AcMc a été étudiée en microscopie optique puis électronique selon des techniques immunocytochimiques (immunopéroxydase indirecte et immunogold). La...
Tipo: Text
Palavras-chave: Mollusque; Bivalve; Mytilus edulis; Cellules neurosécrétrices; Système nerveux; Système immunitaire; Controle de la croissance; Insuline; Anticorps monoclonaux; Immunocytocbimie; ELISA; PCR; Banque d'ADNc.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00109/22019/19642.pdf

Caractérisation moléculaire du parasite du genre Perkinsus infectant les palourdes en France et étude du polymorphisme génétique en fonction de son origine géographique
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Michel, Justine.
En 2004 et 2005, une étude menée par le REPAMO (Réseau de Pathologie des Mollusques) a montré que des parasites du genre Perkinsus étaient présentas au sein des principaux domaines français de production de palourdes: La Mer Méditerranée, l'Océan Atlantique et la Manche avec une forte prévalence amis aucun cas de mortalités associées. Cependant, ces résultats doivent être complétés par des analyses moléculaires plus spécifiques de façon à déterminer la ou les espèces de Perkinsus détectés. D'autre, part, l'étude a montré que différents facteurs environnementaux (température, salinité...) liés à la répartition géographique influencent le développement de parasite. Ces résultats suggèrent l'existence d'une variabilité génétique chez le ou les parasites du...
Tipo: Text
Palavras-chave: Parasite; Perkinsus; PCR; Ruditapes decussatus; Ruditapes philippinarum; Variabilité génétique.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00033/14453/11754.pdf

Validation of host-specific Bacteriodales 16S rRNA genes as markers to determine the origin of faecal pollution in Atlantic Rim countries of the European Union
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Gawler, A; Beecher, J; Brandao, J; Carroll, N; Falcao, L; Gourmelon, Michele; Masterson, B; Nunes, B; Porter, J; Rince, A; Rodrigues, R; Thorp, M; Walters, J; Meijer, W.
The recent implementation of the Revised Bathing Water Directive in the European Union has highlighted the need for development of effective methods to differentiate between sources of faecal contamination. It had previously been shown that amplification of 16S rRNA genes of host-specific Bacteriodales species using the HF183F and CF128F primers could be used as markers for human and bovine faecal contamination in the United States. This paper determined the sensitivity and specificity of these markers in four Atlantic Rim countries (France, Ireland, Portugal and the United Kingdom) to evaluate their usefulness in determining the origin of faecal contamination. It was shown that the HF183F marker displayed high sensitivity (80-100%) and specificity...
Tipo: Text
Palavras-chave: Sensitivity; Specificity; PCR; Microbial source tracking; Bacteriodales.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-4957.pdf

PCR performance of a thermostable heterodimeric archaeal DNA polymerase
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Killelea, Tom; Ralec, Celine; Bosse, Audrey; Henneke, Ghislaine.
DNA polymerases are versatile tools used in numerous important molecular biological core technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome sequencing, and nucleic acid based diagnostics. Taking into account the multiple DNA amplification techniques in use, different DNA polymerases must be optimized for each type of application. One of the current tendencies is to reengineer or to discover new DNA polymerases with increased performance and broadened substrate spectra. At present, there is a great demand for such enzymes in applications, e.g., forensics or paleogenomics. Current major limitations hinge on the inability of conventional PCR enzymes, such as Taq, to amplify degraded or low amounts of template DNA. Besides,...
Tipo: Text
Palavras-chave: DNA polymerase; Archaea; Family D; PCR; Pyrococcus.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00193/30450/28871.pdf

Rôle de la moule, Mytilus edulis, dans la transmission du parasite de l'huître plate, Marteilia refringens
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Faure, Stephanie.
La marteiliose est une maladie causée par Marteilia refringens chez l'huître plate, Ostrea edulis. Ce parasite induit des mortalités importantes chez ces mollusques bivalves. Une autre espèce, Marteilia maurini, parasite la moule, Mytilus edulis, sans engendrer de mortalités notables. Des cas de coinfections des moules par ces 2 parasites ont été observés. La moule pourrait donc être vecteur de Marteilia refringens. Des études sur la taxinomie de Marteilia refringens ont démontré qu'il existe un polymorphisme entre les 2 espèces dans l'ITS1. De ce fait, une technique de PCR-RFLP a été mise au point pour discriminer les 2 espèces. Le but de cette étude est d'éclaircir le statut de la moule dans le cycle biologique de Marteilia refringens par deux approches...
Tipo: Text
Palavras-chave: Marteilia refringens; Marteilia maurini; Mytilus edulis; Vecteur; PCR-RFLP; PCR; Pathologie.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00032/14343/11627.pdf

Rapport de la mission effectuée du 12 au 19/12/97en Nouvelle-Calédonie : - Ecophysiologie et Pathologie
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Soyez, Claude; Avarre, Jean-christophe.
Ecophysiologie L'outil Capacité Osmorégulatrice (CO) mis au point ces dernières années est devenue un moyen performant et largement exploité en routine dans nos programmes de recherche en environnement, en pathologie-immunologie, en génétique et en physiologie de la nutrition. La mise en oeuvre de cet outil nous a permis de répondre à un certain nombre de questions posées dans le cadre de nos objectifs de recherche. Il s'agissait dans un deuxième temps d'éprouver et d'adapter cet outil à des études in situ (dans les bassins d'élevages). C'est dans ce cadre qu'une action a été menée en Nouvelle-Calédonie avec nos collègues de la SASV. En Nouvelle-Calédonie, la chute de température préside à une dégradation du milieu et des conditions d'élevage et...
Tipo: Text
Palavras-chave: Capacité osmorégulatrice; Stress; Environnement; Écophysiologie; Nouvelle-Calédonie; Diagnostic; V. penaeicida; Épidémiologie; PCR.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/00680/79233/81748.pdf

Détection et étude de la stabilité de l'ADN de virus de type herpès infectant les huîtres dans des échantillons d'eau
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Vigneron, Vassilia.
Depuis 1991, des mortalités massives associées à des infections à herpès virus sont rapportées chez des larves et des juvéniles de différentes espèces de bivalves dans différentes régions du globe. Les études ont porté essentiellement sur la mise en évidence de la présence de ce type de virus chez les coquillages d'intérêt économique. Actuellement, l'intérêt se porte sur la persistance du virus dans le milieu marin, et cela dans le but de déterminer si le milieu constitue ou non un moyen de contamination des animaux. Le travail a constitué en une étude sur la persistance de la détection de l'ADN d'herpès virus (OsHV-1) afin de mieux appréhender les modes de dissémination et de transmission du virus. Dans un premier temps, la stabilité de l'ADN purifié et...
Tipo: Text
Palavras-chave: Herpèsvirus; Eau de mer; Détection; PCR; Persistance; Stabilité.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00032/14350/11634.pdf

Étude de la capacité de toxicogenèse de la souche d'Escherichia coli H10407 introduite en eau de mer synthétique
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Jolivet Gougeon, Anne; Tamanai Shacoori, Zohreh; Pommepuy, Monique; Cormier, Michel.
Survival of Escherichia coli H10407 strain, introduced into artificial seawater during exponential and stationary growth phases, was studied for seven days. Pathogenicity of stressed bacteria in these conditions was estimated by detection of the thermolabile toxin LT (excreted in situ in artificial seawater) and the elt-coding gene (always detected). Emergence of viable but non-culturable bacteria appeared as an important stage in the pathogenic evolution of this strain; resuscitation experiments were attempted to confirm the expression of the elt-gene of virulence. This resuscitation was only possible during the first two or three days after stress when the bacteria are still virulent. More than three days after the stress, the bacteria were not able to...
Tipo: Text
Palavras-chave: Bactéries viables non cultivables; Gène elt; Digoxigénine; PCR; Escherichia coli entérotoxinogènes (ETEC); Viable non culturable bacteria; Elt gene; Digoxigenin; PCR; Enterotoxigenic Escherichia coli ETEC.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-524.pdf

Detection of Type 1 Ostreid Herpes variant (OsHV-1 mu var) with no associated mortality in French-origin Pacific cupped oyster Crassostrea gigas farmed in Italy
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Dundon, William G.; Arzul, Isabelle; Omnes, Emmanuelle; Robert, Maeva; Magnabosco, Cristian; Zambon, Michela; Gennari, Lorenzo; Toffan, Anna; Terregino, Calogero; Capua, Ilaria; Arcangeli, Giuseppe.
In April 2010, the sampling of juvenile Pacific cupped oysters originating from France was undertaken from a farm located off the coast of the Marche region in Italy. Samples were sent to the national reference laboratory for mollusc diseases for bacteriological, histological, electron microscopical and molecular analysis. Classical and Real-Time PCR indicated the presence of the microvariant (OsHV-1 mu var) of Type 1 Ostreid Herpes virus (OsHV-1) despite the absence of clinical and pathological signs normally associated with the presence of this variant. Further molecular and sequencing analysis revealed the presence of OsHV-1 par and OsHV-1 DNA in one oyster indicating that simultaneous detection of both viruses is possible. This is the first report of...
Tipo: Text
Palavras-chave: Pacific cupped oyster; Ostreid Herpes virus 1 mu var; PCR; Italy.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00035/14585/11968.pdf

Bonamia ostreae, parasite de l'huître plate, Ostrea edulis: sa position taxonomique parmi les parasites du groupe "microcell", analyses des interactions hôte/parasite chez plusieurs populations d'huîtres plates
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Besnard-cochennec, Nathalie.
Bonamia ostreae is the causative agent of hemocyte disease. It has been detected first during mortalities occured in Franc in 1979. Bonamiosis spread rapidly among oysters farming in France and Europ. Another species, Bonamia sp. has been detected in New Zealand and Australia. These paasites were both included in the "microcell" group. This group contains 2 others parasites, Mikrocytos mackini affecting Crassostrea gigas in British Columbia (Canada) and M. roughleyi affecing Saccostrea glomerata in Australia. Structural and molecular caracterisation of all these parasites were realised. Results show tha B. ostreae, B. sp., B. exitiosus. Molecular tools are available for genus detection, Bonamia spp. and Mikrocytos and species identification (B. ostreae, B....
Tipo: Text
Palavras-chave: Bonamia ostreae; Ostrea edulis; Taxonomie; Diagnotic; PCR; In situ hybridation; Hémocytes; Lectines; Phagocytose; Résistance; Bonamia ostreae; Ostrea edulis; Taxonomy; Diagnotic; PCR; In situ hybridization; Hemocytes; Lectins; Phagocytosis; Resistance.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00211/32223/30671.pdf

First report of a Mikrocytos-like parasite in European oysters Ostrea edulis from Canada after transport and quarantine in France
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Gagne, N; Cochennec, Nathalie; Stephenson, M; Mcgladdery, S; Meyer, G; Bower, S.
As part of a disease resistance experiment, 112 apparently healthy European flat oysters Ostrea edulis L. were exported from Canada (Nova Scotia) into France to test their susceptibility to Bonamia ostreae infection. Twelve oysters died in transit and 17 others died within 2 wk of laboratory quarantine acclimation. All oysters were examined histologically, and the 17 that died during quarantine were assayed for microcells (Bonamia sp. and Mikrocytos mackini) using molecular techniques. A microcell parasite was detected in the connective tissue of 5 of the 112 oysters. Morphological appearance, tissue affinity and molecular characterization through PCR, in situ hybridization (ISH), fluorescence in situ hybridization (FISH) and sequencing revealed a protist...
Tipo: Text
Palavras-chave: Histology; PCR; In situ hybridization; Ostrea edulis; Mikrocytos.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4475.pdf

Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan.
Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1...
Tipo: Text
Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf

New evidence for the involvement of Paracartia grani (Copepoda, Calanoida) in the life cycle of Marteilia refringens (Paramyxea)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boyer, S.; Chollet, Bruno; Bonnet, D.; Arzul, Isabelle.
The dynamics of the protozoan parasite Marteilia refringens was studied in Thau lagoon, an important French shellfish site, for 1 year in three potential hosts: the Mediterranean mussel Mytilus galloprovincialis (Mytiliidae) the grooved carpet shell Ruditapes decussatus (Veneriidae) and the copepod Paracartia grani (Acartiidae). Parasite DNA was detected by PCR in R. decussatus. In situ hybridisation showed necrotic cells of M. refringens in the digestive epithelia of some R. decussatus suggesting the non-involvement of this species in the parasite life cycle. In contrast, the detection of M. refringens in mussels using PCR appeared bimodal with two peaks in spring and autumn. Histological observations of PCR-positive mussels revealed the presence of...
Tipo: Text
Palavras-chave: Marteiliosis; Marteilia refringens; Parasite life cycle; Mytilus galloprovincialis; Ruditapes decussatus; Paracartia grani; PCR; In situ hybridisation.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00157/26795/25532.pdf

Vibrio nigripulchritudo monitoring and strain dynamics in shrimp pond sediments
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Walling, Emilie; Vourey, Elodie; Ansquer, Dominique; Beliaeff, Benoit; Goarant, C.
Aims: A description of bacterial pathogens in shrimp ponds is necessary to understand their pathogenesis. Vibrio nigripulchritudo was shown to contain saprophytic and pathogenic strains among New Caledonian isolates. We established a method to map the development of V. nigripulchritudo in pond sediments at three different genetic levels: the species level, then at the pathogenic cluster level and finally at the plasmid level, present only in all highly pathogenic isolates. Methods and Results: PCR methods were applied to shrimp pond sediments both before and after a mortality outbreak. Using crude samples, the species V. nigripulchritudo is not detected at first (0/42 samples at day 56 post stocking) but appears frequently in the sediments after the...
Tipo: Text
Palavras-chave: Detection; Diagnosis; Diseases; Ecology; PCR; Plasmids; Soil; Virulence.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00004/11484/8177.pdf

A la recherche de l'huître portuguaise: identification de marqueurs moléculaires discriminant Crassostrea angulata et Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Cornette, Florence.
L'étude historique de l'ostréiculture française monte que cette activité est caractérisée par des périodes de prospérités et de pénurie. Des épizooties ont été à l'origine de ces alternances. A chacune de ces période, on a pu constater des changements d'espèces : Ostrea edulis fut remplacée par Crassostrea angulata, de même celle-ci fut remplacée par Crassostrea gigas dans les années 1970. L'étude de la bibliographie montre que les techniques utilisées pour distinguer des espèces Crassostrea angulata et Crassostrea gigas sont insuffisantes. L'évolution des technologies en matière d'analyses génétiques ouvrent de nouvelles perspectives. La recherche de marqueurs moléculaires réalisée sur Crassostrea angulata et Crassostrea gigas, par amplification par PCR...
Tipo: Text
Palavras-chave: Crassostrea angulata; Crassostrea gigas; Marqueurs moléculaires; ADN mitochondrial; PCR; Enzymes de restrictions; Haplotypes.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00049/15979/13421.pdf

Rôle de la moule, Mytilus Edulis dans la transmission du parasite de l'huître plate, Marteilia Refringens
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Mathia, Olivier.
Marteilia refringens est l'un des parasites de l'huître plate, Ostrea edulis ; la marteiliose dont il est l'agent causal provoque une forte mortalité chez les mollusques bivalves. Le genre Marteilia comprend d'autres espèces telle que Marteilia maurini qui lui affecte la moule, Mytilus edulis. Il a été démontré, dans quelques rares cas que les deux espèces pouvaient co habiter au sein d'une même moule. Ainsi le mollusque pourrait être vecteur de Marteilia refringens. Certaines études ont montré par PCR et PCR-RFLP qu'il existait un dimorphisme dans la région ITS-1 des gènes ribosomaux chez Marteilia. Ces techniques m'ont donc permis d'observer la prévalence du phénomène de co-detection dans le milieu naturel et d'étudier l'implication de la moule dans le...
Tipo: Text
Palavras-chave: Marteilia refringens; Marteilia maurini; Mytilus edulis; Vecteur; PCR; PCR-RFLP.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00369/47981/48007.pdf

Needle in a haystack: involvement of the copepod Paracartia grani, in the life-cycle of the oyster pathogen Marteilia refringens
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Audemard, Corinne; Le Roux, Frédérique; Barnaud, Antoine; Collins, Catherine; Sautour, B; Sauriau, Pierre-guy; De Montaudouin, X; Coustau, C; Combes, C; Berthe, Franck.
Marteilia refringens is a major pathogen of the European flat oyster, Ostrea edulis Linnaeus. Since its description, the lifecycle of this protozoan parasite has eluded discovery. Attempts to infect oysters experimentally have been unsuccessful and led to the hypothesis of a complex life-cycle involving several hosts. Knowledge of this life-cycle is of central importance in order to manage oyster disease. However, the exploration of M. refringens life-cycle has been previously limited by the detection tools available and the tremendous number of species to be screened in enzootic areas. In this study, these two restrictions were circumvented by the use of both molecular detection tools and a mesocosm with low biodiversity. Screening of the entire fauna of...
Tipo: Text
Palavras-chave: Experimental transmission; In situ hybridization; PCR; Paracartia grani; Ostrea edulis; Marteilia refringens.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2778.pdf

Contribution à la validation de la technique de PCR pour la détection du parasite Bonamia ostreae chez l'huître plate, Ostrea edulis
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Dubois, Rodolphe.
Bonamia ostreae, l'agent de la bonamiose, est un parasite intracellulaire ayant pour cible les hémocytes (cellules du système de défense) de l'huître plate ostrea edulis. Il induit chez son hôte des lésions branchiales, telles que des perforations ou des indentations pouvant aller jusqu'à la mort du mollusque. Le travail réalisé au cours de ce stage s'inscrivait dans une démarche de validation de méthode de PCR (Polymerase Chain Reaction) pour la détection de ce parasite. Les analyses ont porté sur un lot de trois cent onze huîtres plates ostrea edulis adultes (2-3 ans), issus de captage naturel et provenant de Quiberon (Bretagne). Pour chaque individu, une extraction d'ADN a été réalisée sur un échantillon de coeur et de branchies. Afin de déterminer si...
Tipo: Text
Palavras-chave: Bonamia Ostreae; Agent pathogène; Bonamiose; Hémocytes; Huître plate; Ostrea edulis; PCR; Validation.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00369/47989/48017.pdf

Essais de caractérisation moléculaire des parasites du genre Marteilia (Phylum Paramyxea) à l'aide de l'ADNr
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rodriguez, David.
Les parasites du genre Marteilia sont responsables de graves épizooties et sont retrouvés dans une communauté très variable de mollusques bivalves marins. Toutefois ces protistes restent difficiles à classer à l'intérieur du genre du fait que les données morphologiques sont insuffisantes pour distinguer avec précision deux espèces données. Ceci est le cas pour Martilia refringens, affectant l'huître plate Ostrea edulis ou la moule (Mytilus edulis et Mytilus galloprovincialis), Marteilia sp. et Marteilia maurini affectant les deux espèces de moules. C'est pourquoi, nous avons tenté d'isoler, par PCR, le gène codant pour l'ARN 18S, les ITS1 et ITS2 ainsi que le gène codant pour l'ARN 5,8S des parasites présents chez l'huître plate et la moule (M. edulis),...
Tipo: Text
Palavras-chave: Parasite; Marteilia; PCR; Ostrea edulis; Mytilus edulis; Biologie moléculaire.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00032/14352/11636.pdf

Programme Herpèsvirus. Synthèse des travaux réalisés au Laboratoire IFREMER de La Tremblade - Mars 1996 - Février 1997
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe.
Ce rapport représente la synthèse des travaux réalisés de mars 1996 à février 1997 au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade, dans le cadre de l'étude du virus de type herpès observé chez les huîtres et de la mise au point de méthodes de diagnostic. La majorité des travaux rapportés ici de façon complète, a déjà fait l'objet de comptes rendus intermédiaires.
Tipo: Text
Palavras-chave: Pathologie; Herpèsvirus; Herpesviridae; Huître creuse; Crassostrea gigas; Huître plate; Ostrea edulis; Virus; Détection; ¨Protocole; ADN; PCR.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00165/27663/25838.pdf

Session de formation à l'utilisation de la technique PCR pour le diagnostic de l'infection à Herpèsvirus chez les huîtres
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile.
Parmi les maladies infectieuses observées chez les mollusques bivalves marins, les viroses sont souvent mal connues, en raison d'une certaine inadéquation des techniques d'identification et de diagnostic généralement mises en oeuvre lors de phénomènes de mortalité. En effet, la microscopie photonique a été et reste encore, dans de nombreux laboratoires travaillant sur les pathologies des mollusques, la technique de base pour l'analyse des échantillons. Cette technique reste insuffisante en cas de pathologie d'origine virale si elle n'est pas complétée par d'autres approches (microscopie électronique à transmission, recherche d'effets cytopathogènes sur monocouches cellulaires et réactifs de diagnostic spécifiques). Aucune lignée de mollusques bivalves...
Tipo: Text
Palavras-chave: Pathologie; Technique laboratoire; PCR; Diagnostic; Herpèsvirus; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15501/12870.pdf

A Case Report of a Botulism Outbreak in Beef Cattle Due to the Contamination of Wheat by a Roaming Cat Carcass: From the Suspicion to the Management of the Outbreak
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Le Maréchal, Caroline; Hulin, Olivier; Macé, Sabrina; Chuzeville, Cécile; Rouxel, Sandra; Poëzevara, Typhaine; Mazuet, Christelle; Pozet, Françoise; Sellal, Eric; Martin, Laure; Viry, Alain; Rubbens, Christine; Chemaly, Marianne.
We report a botulism outbreak in Charolais cattle fed with wheat flour contaminated by Clostridium botulinum type C and the management of the outbreak at each step from the clinical suspicion to the cleaning and disinfection operations. Diagnosis was based on typical suggestive clinical signs and detection of C. botulinum type C using real-time PCR in samples collected from three young affected bulls. All young exposed bulls and cows (18 animals) eventually died, but three young bulls and one cow were recovering when it was decided to euthanize them. C. botulinum type C was detected in the liver of these four animals. Analysis of the ration components demonstrated that wheat flour, wheat, and the mill used to make flour were positive for C. botulinum type...
Tipo: Text
Palavras-chave: Cattle botulism; Cat carcass; PCR; Wheat; Clostridium botulinum; Feed contamination.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00603/71516/69936.pdf

Recherche d'AND d'herpèsvirus infectant les bivalves dans des échantillons d'eau de claires ostréicoles
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Solliec, Gaëlle; Vigneron, Valérie; Montanie, Hélène; Renault, Tristan.
Viral particles, associated with high mortality rates, have been reported among larvae and juveniles in different marine bivalve specie and characterized as member of the Hespesviridae family. Most studies focused on the detection of such viral infections among economically important species. However, the persistence of bivalve herpes viruses in marine environment is poorly documented. This topic appears as an important research domain in order to clarify the natural infection cycle of the herpesvirus of marine bivalves.
Tipo: Text
Palavras-chave: Herpesviridae; Oyster; PCR; DNA; Herpesvirus; Huître; PCR; ADN.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-2856.pdf

Comparison of three methods of DNA extraction from cold-smoked salmon and impact of physical treatments
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Giacomazzi, Sophie; Leroi, Francoise; Joffraud, Jean-jacques.
Aims: To compare three bacterial DNA extraction procedures on cold-smoked salmon (CSS) and assess the impact on their efficiency of two physical treatments of the food matrix, ionizing irradiation and freezing.   Methods and Results: As molecular methods for bacterial detection have become an important analytical tool, we compared bacterial DNA extraction procedures on CSS. Working with frozen and irradiated CSS, we obtained negative responses from samples known to be highly contaminated. Thus, we decided to study the impact of these two physical treatments on bacterial DNA extraction procedures. The efficiency of bacterial DNA extraction directly from the fish matrix suspension was measured by an rpoB PCR-based reaction. The results demonstrated that the...
Tipo: Text
Palavras-chave: RpoB gene; PCR; Ionizing irradiation; Freezing; DNA extraction; Cold smoked salmon.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-1106.pdf

Ecologie du copépode calanoïde Paracartia grani. Implication dans le cycle de vie du parasite Marteilia refringens dans la lagune de Thau
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boyer, Séverine.
In this study, the life cycle of Paracartia grani, a calalloid copepod belonging to the Acartiidae family was determined in Thau lagoon. Indeed, the copepod involvement in the life cycle of the parasite Marteilia refringens affecting the bivalve productions is suspected. Mesozooplanktonic community .was monitored twice a month. over two years at a fixed station in the lagoon. Sampling has identified P. grani as the acartiid dominant species in summer. From April to January, the copepod is found in the water column while from February to early April it remains in the sediment as diapausing eggs. The analysis of the population structure (size spectmm, contribution of developmental stages and sex ratio) has revealed that there are in between 6 and 9...
Tipo: Text
Palavras-chave: Paracartia grani; Cycle de vie; Production d'oeufs; Oeufs de diapause; Mésozooplancton; Marteilia refringens; Mytilus galloprovincialisa; Ruditapes decussatus; PCR; Hybridation in situ; Lagune de Thau; Paracartia grani; Life cycle; Egg production; Diapause eggs; Mesozooplankton; Marteilia refringens; Mytilus galloprovincialis; Ruditapes decussatus; PCR; In situ hybridization; Thau lagoon.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00118/22931/21482.pdf

Detección Molecular de Estreptococos Cariogénicos en Saliva
Provedor de dados: International Journal of Morphology
Autores: Salazar,Luis A; Vásquez,Claudio; Almuna,Alejandro; Oporto,Gonzalo; Santana,Roberto; Herrera,Christian L; Sanhueza,Antonio.
Streptococcus mutans y Streptococcus sobrinus han sido indicados como los principales agentes etiológicos de la caries dental. Sin embargo, los métodos microbiológicos y bioquímicos, disponibles actualmente en Chile, no permiten la rápida detección e identificación de estas bacterias. El objetivo de este trabajo fue implementar la metodología de reacción en cadena de la polimerasa (PCR) para detectar la presencia de S. mutans y S. sobrinus en saliva. Participaron de este estudio 51 escolares (5 a 17 años), provenientes de cinco diferentes colegios de la ciudad de Temuco; a los cuales se les realizó recuento de estreptococos del grupo mutans en saliva por método microbiológico y la diferenciación de especies por la técnica de PCR. Los resultados mostraron...
Tipo: Journal article
Palavras-chave: Caries; Streptococcus mutans; PCR.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400027

Estudio de factores de riesgo asociados a la infección por Mycoplasma suis
Provedor de dados: Investigación Veterinaria
Autores: Pereyra,N.B.; Pérez,A.M.; Messick,J.B.; Cane,F.D.; Guglielmone,A.A.
Este estudio se propuso estimar la distribución de la infección por Mycoplasma suis en poblaciones de cerdos de Argentina e identificar factores de riesgo asociados. Se recolectaron 284 muestras de sangre de cerdos de diferentes categorías productivas en frigoríficos y granjas de las provincias de Santa Fe, Córdoba y Buenos Aires. Amplificando el gen del ARNr 16S de M. suis a través de la reacción en cadena de la polimerasa (PCR), se calculó un porcentaje de infectados del 64%. Se estimó además que no existía asociación estadísticamente significativa (p&amp;gt;0,1) entre un resultado positivo a la PCR y el sexo del animal muestreado, los antecedentes de anemia en la granja y las condiciones de alojamiento. Contrariamente se encontró asociación...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma suis; PCR; Factores de riesgo; Cerdos.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982010000200001

Leptospira spp. infection in sheep herds in southeast Brazil
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Barbante,Priscila; Shimabukuro,Fabio H; Langoni,Helio; Richini-Pereira,Virgínia B; Lucheis,Simone B.
Background With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp.Results MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Ovine; Serology; Culture; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200325

Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Marconi,C.; Cunha,M. L. R. S.; Araújo Jr,J. P.; Rugolo,L. M. S. S.
Coagulase-negative staphylococci (CNS), components of the normal flora of neonates, have emerged as important opportunistic pathogens of nosocomial infections that occur in neonatal intensive care units. Some authors have reported the ability of some CNS strains, particularly Staphylococcus epidermidis, to produce a toxin similar to S. aureus delta toxin. This toxin is an exoprotein that has a detergent action on the membranes of various cell types resulting in rapid cell lysis. The objectives of the present study were to standardize the Polymerase Chain Reaction (PCR) technique for the detection of the gene responsible for the production of delta toxin (hld gene) in staphylococcal species isolated from catheters and blood cultures obtained from neonates,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Delta toxin; PCR; Staphylococcus; Coagulase-negative staphylococci.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200004

Molecular detection ofLeishmaniaspp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Richini-Pereira,Virginia Bodelão; Marson,Pamela Merlo; Hayasaka,Enio Yoshinori; Victoria,Cassiano; Silva,Rodrigo Costa da; Langoni,Hélio.
Background Road-killed wild animals have been classified as sentinels for detecting such zoonotic pathogens asLeishmaniaspp., offering new opportunities for epidemiological studies of this infection.Methods This study aimed to evaluate the presence ofLeishmaniaspp. andLeishmania chagasiDNA by PCR in tissue samples (lung, liver, spleen, kidney, heart, mesenteric lymph node and adrenal gland) from 70 road-killed wild animals.Results DNA was detected in tissues of oneCavia aperea(Brazilian guinea pig), fiveCerdocyon thous(crab-eating fox), oneDasypus septemcinctus(seven-banded armadillo), twoDidelphis albiventris(white-eared opossum), oneHydrochoerus hydrochoeris(capybara), twoMyrmecophaga tridactyla(giant anteater), oneProcyon cancrivorus(crab-eating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Road-killed animal; Leishmaniaspp; Leishmania chagasi; PCR; Zoonosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200331

Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Costa,VM; Langoni,H.
The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Parasite in milk; Female rats; PCR; Bioassay.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200016

Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Langoni,H.; Das Dores,C. B.; Silva,R. C.; Pezerico,S. B.; Castro,A. P. B.; Da Silva,A. V.
Detection of Toxoplasma gondii (T. gondii) DNA in blood can help to diagnose the disease in its acute phase; however, it must be considered that hemoglobin, present in blood, can inhibit polymerase activity, making impracticable the detection of DNA in samples. Mice were experimentally infected via oral route with ME49 and BTU2 strains cysts and RH strain tachyzoites; polymerase chain reaction was used to detect T. gondii DNA in mice sera 18, 24, 48, 96, and 192 hours post infection (PI). Toxoplama gondii DNA was detected in only one animal infected with BTU2 strain, genotype III (isolated from a dog with neurological signs) 18 hours PI. The agent's DNA was not detected in any sample of the other experimental groups. New studies must be carried out to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmosis; PCR; Serum; Experimental infection; Mice.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200004

Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Richini-Pereira,VB; Bosco,SMG; Theodoro,RC; Barrozo,L; Bagagli,E.
Road-killed wild animals have been for years used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies. In the current study, fungal infection was evaluated by PCR and nested-PCR in tissue samples collected from 19 road-killed wild animals. The necropsies were carried out and samples were collected for DNA extraction. Results, using PCR with a panfungal primer and nested PCR with specific primers, indicated that some animals are naturally infected with Amauroascus aureus, Metarhizium anisopliae, Aspergillus flavus, Aspergillus oryzae, Emmonsia parva, Paracoccidioides brasiliensis or Pichia stipitis. The approach employed herein proved useful for detecting the environmental occurrence of several...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Road-killed animals; Fungal pathogens; Eco-epidemiology; PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400011

Association among H. pylori virulence markers dupA, cagA and vacA in Brazilian patients
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Pereira,Weendelly Nayara; Ferraz,Mariane Avante; Zabaglia,Luanna Munhoz; Labio,Roger William de; Orcini,Wilson Aparecido; Ximenez,João Paulo Bianchi; Caleman Neto,Agostinho; Payão,Spencer Luiz Marques; Rasmussen,Lucas Trevizani.
Background:Only a few Helicobacter pylori-infected individuals develop severe gastric diseases and virulence factors of H. pyloriappear to be involved in such clinical outcomes. Duodenal ulcer promoting gene A (dupA) is a novel virulence factor ofHelicobacter pylori that is associated with duodenal ulcer development and reduced risk for gastric carcinoma in some populations. The aims of the present study were to determine the presence ofdupA gene and evaluate the association amongdupA and other virulence factors includingcagA and vacA in Brazilian patients. Gastric biopsies were obtained from 205 dyspeptic patients (100 children and 105 adults). DNA was extracted and analyzed for the presence of H. pylori and its virulence factors using the polymerase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; DupA; CagA; VacA; Gastroduodenal diseases; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200319

Polymerase chain reaction in detecting Leishmania sp in symptomatic and asymptomatic seropositive dogs
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Soares,M. J. V.; Moraes,J. R. E.; Roselino,A. M. F.
In human and canine renal histological studies of visceral leishmaniasis (VL), the etiological agent is rarely found in situ. The objective of this study was to evaluate PCR in identifying the etiological agent in spleen, liver, lymph node, and kidneys of VL-seropositive dogs. Twenty-five symptomatic (case group) and 15 asymptomatic (control group) VL-seropositive dogs of different breeds, sexes, and ages from Teresina, Piauí State, Brazil, were used. Serologic diagnosis was made by enzyme-linked immunosorbent assay and indirect immunofluorescence test. Animals were subjected to euthanasia and necropsy. Renal fragments were immersed in buffered formaldehyde solution. Spleen, liver, lymph node, and kidney samples were collected and frozen at -70ºC until DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dogs; Leishmania; PCR; Kidney.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400011

Diagnosis of Giardia infections by PCR-based methods in children of an endemic area
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: David,EB; Coradi,ST; Oliveira-Sequeira,TCG; Ribolla,PEM; Katagiri,S; Guimarães,S.
The present study was designed to estimate the prevalence of Giardia infection in preschool- and school-aged children living in an endemic area. Fecal samples from 573 children were processed by zinc sulfate centrifugal flotation, centrifugal sedimentation (using a commercial device for fecal concentration - TF-Test kit®) and polymerase chain reaction (PCR)-based methods. Of the stool samples assessed, 277 (48.3%) were positive for intestinal parasites and/or commensal protozoa. Centrifugal flotation presented the highest diagnostic sensitivity for Giardia infections. The kappa index revealed that both coproparasitological techniques closely agreed on the Giardia diagnosis (86%) versus satisfactory (72%) and poor (35%) concordances for commensal protozoan...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Giardia duodenalis; Children; Diagnosis; PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200012

Characterization of Clostridium perfringens isolated from mammals and birds from Guwahati city, India
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Rahman,Mafruza S; Sharma,RK; Borah,P; Chakraborty,A; Devi,Mandakini RK; Longjam,N.
Of the 102 samples collected from mammals and birds, both domestic and captive wild, 48 were found to be positive for Clostridium perfringens. Most of the mammal isolates (84.38%) appeared to have been collected from clinically affected animals, while 33.33% of the bird samples were from clinically affected and 21.43% from apparently healthy birds infected with C. perfringens. Isolates revealed high sensitivity to ciprofloxacin, enrofloxacin and norfloxacin. Among the isolated C. perfringens, 30 (62.50%) showed DNase production. Hemolytic activity was recorded in 14 (24.16%) of the isolates and 28 (58.33%) showed phospholipase C production. All the phospholipase C positive isolates revealed the presence of cpa gene encoding alpha (α) toxin. Of the 102...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; PCR; Α toxin; Cpa gene; Polymerase chain reaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100010

Detection of bovine Clostridium perfringens by polymerase chain reaction
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Piatti,R. M.; Ikuno,A. A.; Baldassi,L.
A polymerase chain reaction (PCR) assay to detect Clostridium perfringens alpha toxin gene (cpa) was used to identify eighty-nine C. perfringens strains obtained from bovine clinical material. The strains were biochemically characterized as C. perfringens. The isolated strains were cultured on plates containing brain heart infusion agar with 5% sheep blood under anaerobic conditions. DNA extraction was performed by boiling. The 324 bp amplification product of cpa was observed in all isolates. C. sordellii, C. botulinum, C. novyi, and C. septicum were also tested but did not produce any alpha toxin gene amplification. These findings suggest that PCR is a useful assay in identifying C. perfringens toxin types.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; PCR; Alpha toxin; Polymerase chain reaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200005

Organ tropism during the acute and chronic phases of Trypanosoma cruzi infection in BALB/c mice
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Oliveira,LRC; Picka,MCM; Nicolete,VC; Calvi,SA; Marcondes-Machado,J.
The aim of the present study was to investigate the presence of Trypanosoma cruzi in the heart, liver, lung, and kidneys, using hemoculture and PCR analysis, of mice infected with different parasite strains during the acute and chronic phases of infection. Parasitemia curves revealed strain-specific biological behaviors. For the Y and JLP strains, the acute phase of infection started at days six and ten post-infection, parasitemia peaked at days seven and 15 post-infection, the chronic phase started at days nine and 28 post-infection, and animals started dying at days 19 and 120 post-infection, respectively. When the two strains were compared, the JLP strain exhibited reduced and slower replication rates associated with a delayed peak of parasitism and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; PCR; Hemoculture; Organs; Distribution.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100005

Genotyping of Clostridium perfringens associated with sudden death in cattle
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Miyashiro,S; Baldassi,L; Nassar,AFC.
Toxigenic types of Clostridium perfringens are significant causative agents of enteric disease in domestic animals, although type E is presumably rare, appearing as an uncommon cause of enterotoxemia of lambs, calves and rabbits. We report herein the typing of 23 C. perfringens strains, by the polymerase chain reaction (PCR) technique, isolated from small intestine samples of bovines that have died suddenly, after manifesting or not enteric or neurological disorders. Two strains (8.7%) were identified as type E, two (8.7%) as type D and the remainder as type A (82.6%). Commercial toxoids available in Brazil have no label claims for efficacy against type E-associated enteritis; however, the present study shows the occurrence of this infection. Furthermore,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Iota toxin; Sudden death; PCR; Cattle.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300010

A study on the genetic diversity of Androctonus crassicauda (Olivier, 1807; Scorpiones: Buthidae) from Turkey
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Ozkan,O; Ahmet,C; Zafer,K.
Mitochondrial DNA (mtDNA) has been widely employed in phylogeographic and phylogenetic studies. In the present study, the genetic identification of the scorpion Androctonus crassicauda (Olivier, 1807) was carried out by using the 16S mitochondrial gene, since this scorpion represents the most important species in Turkey regarding scorpionism and antivenom production. Two genetic groups were found according to the sequence analysis results, while five different loci at the nucleotide level presented genetic variation in the 16S region when compared to a known A. crassicauda sequence data (GenBank, AJ277598). Nucleotide variations found in the current work constitute the first descriptive report for A. crassicauda. Moreover, future studies may enlighten the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion; Androctonus crassicauda; PCR; Genotype; Turkey.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400010

Relationship between Helicobacter pylori detection and an increased risk of infection in childhood
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Venerando,R; Rasmussen,LT; de Labio,RW; Gatti,LL; Francisco,O; Viani,GA; Rivera,LML; Payão,SLM.
The risk of developing gastric cancer is believed to be related to differences among Helicobacter pylori strains and the inflammatory responses mediated by host genetic factors. H. pylori infection is acquired at an early age and in the absence of appropriate antibiotic therapy, it generally persists for life. Tp53 gene regulates the transcription of several cytokines and chemokines involved in innate immunity and its action may be influenced by the presence of different H. pylori strains. The present study aimed to detect H. pylori in pediatric patients, to access Tp53 polymorphism at codon 72 and to correlate such findings with age and histopathological results. Three hundred and forty-two patients were analyzed. DNA from their gastric biopsies was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Childhood; Tp53 polymorphims; PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400004

Polyphasic re-examination of Debaryomyces hansenii strains and reinstatement of D. hansenii, D. fabryi and D. subglobosus
Provedor de dados: Naturalis Digital Academic Repository
Autores: Groenewald, M.; Daniel, H.-M.; Robert, V.; Poot, G.A.; Smith, M.Th.
The type species of the genus Debaryomyces, Debaryomyces hansenii, is a highly heterogeneous species. It has been isolated from a large diversity of natural sources including fruit, air, water, soil, but most frequently from processed food products. The species delineation of this yeast species urgently needs clarification. The heterogeneity in taxonomic characteristics has resulted in the description of varieties linked to D. hansenii. The aim of this study was to re-examine and classify D. hansenii using a polyphasic approach. A total of 65 D. hansenii isolates were examined, 57 representing the variety hansenii and nine the variety fabryi. The selection of strains for DNA reassociation and phylogenetic analysis was based on polymerase chain reaction...
Tipo: Article / Letter to the editor
Palavras-chave: ACT1; Debaryomyces fabryi; Debaryomyces hansenii; Debaryomyces subglobosus; DNA reassociation; Maximal growth temperature; PCR; Ingerprinting; Taxonomy.
Ano: 2008
URL: http://www.repository.naturalis.nl/record/531832

Effect of Baculovirus spodoptera isolates in Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD
Provedor de dados: Neotropical Entomology
Autores: Barreto,Marliton R.; Guimaraes,Cláudia T.; Teixeira,Flávia F.; Paiva,Edilson; Valicente,Fernando H.
The total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th and 7th day after virus ingestion. The 22 Baculovirus isolates were used in six concentrations (from 10³ to 10(8) polyhedra/ml) and one check treatment with water. Mortality rate, larval period, pupal period, pupa weight and lethal concentration (LC50) were determined for all isolates. Significant differences were found among all isolates and different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insect pathology; Fall armyworm larvae; PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100010

Entomological characterization and natural infection of anophelines in an area of the Atlantic Forest with autochthonous malaria cases in mountainous region of Espírito Santo State, Brazil
Provedor de dados: Neotropical Entomology
Autores: Rezende,Helder R; Soares,Renata M; Cerutti Jr,Crispim; Alves,Isabel C; Natal,Delsio; Urbinatti,Paulo R; Yamasaki,Tasciane; Falqueto,Aloísio; Malafronte,Rosely dos S.
Autochthonous malaria cases in the state of Espírito Santo, Brazil, are distributed in mountainous regions surrounded by the Atlantic Forest. While some aspects of this disease are unclear, detection of possible vector species can help to elucidate epidemiological uncertainties. Entomological and natural infection studies were carried out using anophelines (Diptera: Culicidae) captured in the municipality of Santa Tereza, ES. Monthly captures were made from March 2004 to February 2006. CDC-CO2 traps were used from dusk (6:00 P.M.) to dawn (6:00 A.M.) to capture anophelines in the following habitats: near the houses, in open areas (at ground level) and inside, and at the margins of the forest (canopy and ground level). Shannon light traps were also used at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anopheles cruzii; Autochthonous malaria; Plasmodium; PCR; Ecology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2009000200017

Comparison of PCR with Parasitology and Serology in the Diagnosis of a Low Virulent Strain of Trypanosoma brucei gambiense in mice
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Mbati, P. A.; Hirumi, K.; Inoue, Noboru; Situakibanza, N. H.; Hirumi, Hiroyuki; 井上, 昇.
Palavras-chave: Trypanosoma brucei gambiense; Mice; Mel-B; Diagnosis; PCR.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/310

Diagnosis of Babesia caballi Infection in Horses by Polymerase Chain Reaction
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇.
A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi,  and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction（PCR）method．The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001％. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting...
Palavras-chave: Babesia caballi; PCR; IFAT.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281

Experimental inoculation of beagle dogs with Ehrlichia species detected from Ixodes ovatus
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Watanabe, Malaika; Oikawa, T; Hiraoka, Hiroko; Kaneko, N; Itamoto, Kazuhito; Mizuno, Tohru; Okuda, Masaru; Inokuma, Hisashi; 猪熊, 壽.
Three beagle dogs were inoculated with mice spleen/liver homogenate infected with Ehrlichia species detected from Ixodes ovatus (EIO) and one dog was used as a control. All three infected dogs did not show clinical signs of disease except for mild pyrexia throughout the 41-day study period. Splenomegaly was observed from Day 7 post-inoculation (p.i.) in two of the dogs. Hematological and biochemical abnormalities included mild thrombocytopenia, hypoproteinaemia, hypoalbuminaemia and increased C-reactive protein values. One of the dogs' splenic aspirate sample was PCR-positive for Ehrlichia Day 7 p.i. and another dogs' blood and bone marrow aspirate sample was PCR-positive Day 41 p.i. Sequence analysis of the PCR products showed 100% homology with the...
Palavras-chave: CANINE MONOCYTIC EHRLICHIOSIS; YAMAGUCHI PREFECTURE; INFECTION; JAPAN; DIAGNOSIS; KINETICS; TICKS; PCR.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/65

Construction of Polymerase Chain Reaction Primer to Detect Cryptosporidium parvum or C. muris
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Zhiliang, Wu; Iseki, Motohiro; Kimata, Isao; Dong, Xu; Nagano, Isao; Takahashi, Yuzo.
Palavras-chave: Cryptosporidium parvum; Cryptosporidium muris; PCR; Primer.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/236

Seroprevalence of Babesia caballi and Theileria equi in horses and mules  from Northern Thailand
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Kamyingkird, K.; Yangtara, S.; Desquesnes, M.; Cao, S.; Adjou Moumouni, P.K.; Jittapalapong, S.; Nimsupan, S.; Terkawi, M.A.; Masatani, T.; Nishikawa, Y.; Igarashi, I.; Xuan, X.
Palavras-chave: Babesia caballi; ELISA; IFAT; PCR; Theileria equi.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4092

Prenatal Infections with Theileria sergenti in Calves
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Onoe, Sadao; Sugimoto, Chihiro; Tanaka, Masayuki; Kubota, Shuichi; Hirai, Tsunao; Yonemichi, Hiromi; Mori, Kiyokazu; Onuma, Misao.
Palavras-chave: Theileria seregenti; Piroplasma; PCR; Prenatal infection.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/213

Molecular Diagnosis of Infective Endocarditis Caused by Streptococcus canis in a Dog
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 田川, 道人; 大橋, 英二; 本村, 絹代; 千葉, 史織; 堀内, 雅之; 三好, 雅史; 猪熊, 壽; 松本, 高太郎; TAGAWA, Michihito; OHASHI, Eiji; MOTOMURA, Kinuyo; CHIBA, Shiori; HORIUCHI, Noriyuki; MIYOSHI, Masahumi; INOKUMA, Hisashi; MATSUMOTO, Kotaro.
10歳の雄の雑種犬が，3週間前から持続する発熱及び元気消失を主訴に帯広畜産大学動物医療センターを受診した．心雑音及び心エコー検査において僧帽弁の疣贅物が確認され，抗生物質等により加療したが，第7病日より心不全の悪化を認め，第10病日に肺水腫により斃死した．病理解剖では，軽度の心拡大及び僧帽弁における多中心性疣贅物が認められた．血液培養検査では，Staphylococcus haemolyticusが検出されたが，疣贅物を用いたPCR検査ではStreptococcus canisと100％一致する塩基配列が得られた．血液培養分離菌はコンタミネーションであった可能性が高く，本症例の心内膜炎はStreptococcus canisによるものと考えられた．血液培養結果と実際の心内膜炎起因菌が異なる場合があることを考慮する必要がある．
Palavras-chave: 心内膜炎; PCR; Streptococcus canis.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4232

Epidemiological Survey of Theileria Parasite Infection of Cattle in Northeast China by Allele-Specific PCR
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: YU, Longzheng; ZHANG, Shoufa; LIANG, Wanfeng; JIN, Chunmei; JIA, Lijun; LUO, Yuzi; LI, Yan; CAO, Shinuo; YAMAGISHI, Junya; NISHIKAWA, Yoshifumi; KAWANO, Suguru; FUJISAKI, Kozo; XUAN, Xuenan; 西川, 義文; 玄, 学南.
An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China.
Palavras-chave: Major piroplasm surface protein; PCR; Theileria.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3586

PCR Detection and Genetic Diversity of Bovine Hemoprotozoan Parasites in Vietnam
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: SIVAKUMAR, Thillaiampalam; LAN, Dinh Thi Bich; LONG, Phung Thang; YOSHINARI, Takeshi; TATTIYAPONG, Muncharee; GUSWANTO, Azirwan; OKUBO, Kazuhiro; IGARASHI, Ikuo; INOUE, Noboru; XUAN, Xuenan; YOKOYAMA, Naoaki; 井上, 昇; 玄, 学南; 横山, 直明.
Hemoprotozoan infections often cause serious production losses in livestock. In the present study, we conducted a PCR-based survey of Babesia bovis, Babesia bigemina, Theileria annulata, Theileria orientalis, Trypanosoma evansi and Trypanosoma theileri, using 423 DNA samples extracted from blood samples of cattle (n=202), water buffaloes (n=43), sheep (n=51) and goats (n=127) bred in the Hue and Hanoi provinces of Vietnam. With the exception of T. annulata and T. evansi, all other parasite species (B. bovis, B. bigemina, T. orientalis and T. theileri) were detected in the cattle populations with B. bovis being the most common among them. Additionally, four water buffaloes and a single goat were infected with B. bovis and B. bigemina, respectively. The Hue...
Palavras-chave: Epidemiology; Hemoprotozoa; Livestock; PCR; Vietnam.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4015

Specific Molecular Detection and Characterization of Anaplasma marginale in Mongolian Cattle
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: YBAÑEZ, Adrian Patalinghug; SIVAKUMAR, Thillaiampalam; BATTSETSEG, Badgar; BATTUR, Banzragch; ALTANGEREL, Khukhuu; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
Anaplasma marginale is an etiologic agent of bovine anaplasmosis. This study aimed to molecularly detect and characterize A. marginale that is prevalent in Mongolian cattle populations. A highly specific and sensitive nested PCR (nPCR) method based on the Msp5 gene was developed to detect A. marginale (Msp5 nPCR). The method detected A. marginale from the positive DNA samples obtained from different countries, while no amplicons were observed from DNA samples of several other bovine blood pathogens tested. The detection limit of Msp5 nPCR was determined to be 2 copies/μl. The method was tested against field blood DNA samples prepared from 300 Mongolian cattle in 2010. Results indicated a prevalence rate of 8.7% (26 of 300). On the other hand, partial DNA...
Palavras-chave: Anaplasma marginale; Cattle; Mongolia; Msp5; PCR.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3867

PCR detection of Babesia microti and Babesia divergens in ticks
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Skotarczak, Bogumila; ACichocka, Gnieszka.
We investigated the presence of Babesia microti and B. divergens in Ixodes ricinus ticks collected in a Lyme borreliosis endemic area of Northern Poland. We used as the PCR target the fragment from a gene encoding the nuclear small-subunit ribosomal RNA (SS-rDNA). We have made a thorough analysis of the quantitative and rate per cent occurrence Ixodes ricinus from two areas of forest in province of Szczecin, known as highly recreative and frequented of many people resort. A total of 533 specimens collected in 13.3% was detected DNA of B. microti and in 3.0% of B. divergens.
Palavras-chave: Babesia microti; Babesia divergens; Ixodes ricinus; PCR.
Ano: 2001
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/135

First Molecular Characterization of Anaplasma marginale in Cattle and Rhipicephalus (Boophilus) microplus Ticks in Cebu, Philippines
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: YBAÑEZ, Adrian Patalinghug; SIVAKUMAR, Thillaiampalam; YBAÑEZ, Rochelle Haidee Daclan; RATILLA, Jowarren Catingan; PEREZ, Zandro Obligado; GABOTERO, Shirleny Reyes; HAKIMI, Hassan; KAWAZU, Shin-ichiro; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 河津, 信一郎; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
Anaplasma marginale has been detected in the Philippines only by peripheral blood smear examination and serological methods. This study generally aimed to molecularly detect and characterize A. marginale in cattle and ticks in Cebu, Philippines. A total of 12 bovine blood samples and 60 Rhipicephalus (Boophilus) microplus ticks were collected on the Cebu Island in 2011. 16S rRNA-based screening-PCR and DNA sequencing revealed 8 cattle (66.7%) and 8 ticks (13.3%) to be positive for A. marginale, and 1 tick (1.7%) to be positive for A. centrale. Selected positive DNA samples were further characterized based on 16S rRNA (longer sequence), Msp5, Msp1α,gltA and groEL genes for phylogenetic analyses. Sequence identities of partial DNA fragments of A. marginale...
Palavras-chave: Anaplasma marginale; Cattle; PCR; Philippines; Rhipicephalus (Boophilus) microplus.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3866

犬におけるパピローマウイルス関連皮膚疾患の病理学的並びに分子病理学的解析
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 古林, 与志安; KOBAYASHI, YOSHIYASU.
2007年度～2008年度科学研究費補助金基盤研究(C) 研究成果報告書19580365
Palavras-chave: 病理・病態; 犬; 色素性表皮母斑; 表皮過誤腫; パピローマウイルス; パラフィン材料; PCR; In situ Hybridization.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2751

Sequence of ITS-2 Amplified from Pearl Millet Downy Mildew Samples
Provedor de dados: Open Acess Agriculture Research Repository
Autores: Viswanathan, A.
Palavras-chave: DNA; PCR; Genetic techniques; Sampling; Ribosomes; Fungi; Genetic variation; Genes; Millets; Cloning.
Ano: 2005
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3262

Working together on groundnut virus diseases
Provedor de dados: Open Acess Agriculture Research Repository
Autores: D.V.R.
Palavras-chave: Viruses; Groundnuts; RNA; Genes; ELISA; Genotypes; Transgenics; PCR; Genetic structures.
Ano: 1994
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4178

Working Together on Groundnut Virus Diseases
Provedor de dados: Open Acess Agriculture Research Repository
Autores: D.V.R.( editor).
Palavras-chave: Viruses; Groundnuts; RNA; Genes; ELISA; Genotypes; Transgenics; PCR; Genetic structures.
Ano: 1994
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3121

Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea)
Provedor de dados: Open Acess Agriculture Research Repository
Autores: Luu M Cuc.
Palavras-chave: Genomes; Genetic structures; Polymorphism; Alleles; DNA; Genotypes; PCR; Cloning; Locus; Groundnuts.
Ano: 2008
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3317

Development of new simple sequence repeat markers for pearl millet
Provedor de dados: Open Acess Agriculture Research Repository
Autores: Yadav, O.P.
Palavras-chave: Locus; DNA; Genetic structures; PCR; Millets; Polymorphism; Genomes; Biotechnology; Breeds (animals); Melting.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3309

Genetic structure, diversity, and allelic richness in composite collection and reference set in chickpea (Cicer arietinum L.)
Provedor de dados: Open Acess Agriculture Research Repository
Autores: Upadhyaya, H.D.
Palavras-chave: Alleles; Chickpeas; Locus; Genomes; Polymorphism; Genes; Genetic structures; DNA; PCR; Peas.
Ano: 2008
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3334

Groundnut bacterial wilt: past, present, and future
Provedor de dados: Open Acess Agriculture Research Repository
Autores: V.K.
Palavras-chave: Bacteria; Groundnuts; Pathogens; PCR; DNA; Enzymes; Genotypes; Oil crops; Mutants.
Ano: 1994
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4181

An effective method for cloning of partial MADS-box genes related to flower development in groundnut
Provedor de dados: Open Acess Agriculture Research Repository
Autores: Yuan, M.
Palavras-chave: Genes; PCR; DNA; Cloning; Transcription factors; Genomes; Transcription; Genetic structures; Nucleotides; Molecular cloning.
Ano: 2005
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3213

Fatores de risco na transmissão do lentivírus caprino pelo sêmen
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Andrioli,Alice; Gouveia,Aurora Maria Guimarães; Martins,Almir de Sousa; Pinheiro,Raymundo Rizaldo; Santos,Diones Oliveira.
O objetivo deste trabalho foi avaliar a presença do DNA pró-viral do lentivírus caprino (LVC) em ejaculados de machos infectados naturalmente, e verificar a influência da lavagem do sêmen e da presença de inflamação testicular sobre a carga viral. Foram realizadas oito coletas de sêmen de sete reprodutores soropositivos para o LVC: quatro antes dos animais sofrerem dano testicular e quatro depois. Entre as coletas realizadas na mesma semana, em uma, o ejaculado era lavado, para retirada do plasma seminal, e na outra, não. O DNA pró-viral do LVC foi identificado pela reação em cadeia da polimerase Nested (PCR Nested), e pelo isolamento viral. O vírus foi isolado em 7,1% das amostras. A PCR identificou o DNA pró-viral em 35,7% do total das amostras: 17,9%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reprodução; PCR; Artrite-encefalite caprina; Transmissão.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000800015

Amplification of 16S rRNA gene sequences to differentiate two highly related bradyrhizobia species
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Giongo,Adriana; Ambrosini,Adriana; Freire,João Ruy Jardim; Zanettini,Maria Helena Bodanese; Passaglia,Luciane Maria Pereira.
A 16S rRNA gene PCR-based assay was developed aiming at a fast molecular diagnostic method to differentiate the two phylogenetically closely related species Bradyrhizobium japonicum and B. elkanii, isolated from soybean nodules, in order to identify those more competitive and comprising greater nitrogen fixation ability for use in the formulation of commercial inoculants. The assay used was able to discriminate ten reference strains belonging to these two Bradyrhizobium species, as well as to efficiently identify 37 strains isolated from fields cultivated with soybean.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Rhizobial diversity; Commercial inoculants.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000900019

Identificação dos genes Ty-2 e Ty-3 de resistência a begomovírusem genótipos de tomateiro
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Aguilera,Jorge González; Hurtado,Francisco Dueñas; Xavier,Cesar Augusto Diniz; Laurindo,Bruno Soares; Nick,Carlos; Gil,Marta Álvarez; Silva,Derly José Henriques da; Zerbini,Francisco Murilo.
O objetivo deste trabalho foi identificar a presença dos genes Ty-2 e Ty-3, de resistência a begomovírus, em acessos de tomateiro do Banco de Germoplasma de Hortaliças da Universidade Federal de Viçosa. Os oligonucleotídeos TO302 F/R e FLUW25 F/R foram utilizados em reações de PCR, para verificar a presença de marcadores relacionados aos genes Ty-2 e Ty-3, respectivamente. Observou-se a presença do gene Ty-2, em heterozigose na subamostra BGH-6881 (Solanum peruvianum), e do gene Ty-3, em homozigose nas subamostras BGH-6878, BGH-6897 (S. lycopersicum) e em heterozigose na subamostra BGH-6881. A identificação dos genes de resistência, com reações de PCR, representa um avanço para os programas de melhoramento de tomateiro no Brasil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum lycopersicum; Solanum peruvianum; Geminivírus; PCR; Recursos genéticos.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000700014

Alterações microbianas no solo durante o ciclo do milho nos sistemas plantio direto e convencional
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Vargas,Luciano Kayser; Selbach,Pedro Alberto; Sá,Enílson Luiz Saccol de.
A disponibilidade de resíduos de aveia-preta, com relação C:N elevada, resulta em imobilização microbiana de nitrogênio no solo, exigindo cuidados no manejo da adubação nitrogenada da cultura subseqüente. O objetivo deste trabalho foi avaliar as alterações na estrutura da comunidade microbiana ao longo do ciclo do milho, na presença de resíduos de aveia-preta e da aplicação de nitrogênio. Foram coletadas amostras de um Argissolo Vermelho distrófico no dia da semeadura do milho e 46, 62, 88 e 112 dias após a semeadura. O nitrogênio foi aplicado 25 dias e 49 dias após a semeadura. As alterações na comunidade microbiana foram avaliadas mediante relações entre carbono (C) e nitrogênio (N), nitrogênio reativo com ninidrina (N-Nin) e carboidratos (CHO) da...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomassa microbiana; PCR; Preparos de solo; Imobilização de nitrogênio; Zea mays.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000800004

Clasificación y estimación de la diversidad genética de Nopal Opuntia spp en base a descriptores fenotípicos y marcadores genético moleculares
Provedor de dados: Phyton: Revista Internacional de Botánica Experimental
Autores: García-Zambrano,Eduardo A; Gutiérrez-Díez,Adriana; Salinas-García,Gilberto E; Cárdenas-Cerda,Elizabeth; Vázquez-Alvarado,Rigoberto E; Zavala-García,Francisco; Martínez de la Cerda,Jesús.
El objetivo de esta investigación fue desarrollar y aplicar marcadores moleculares de tipo RAPD para la estimación de la diversidad genética de 100 accesiones de nopal que forman el Banco de Germoplasma de la FAUANL. Los datos moleculares fueron evaluados por medio del análisis multivariado, utilizando los métodos de Ward y UPGMA; estos resultados se compararon con datos fenotípicos tomados de las mismas accesiones, evaluados por los métodos ya mencionados. Para lograr este objetivo se modificó el protocolo para la extracción del DNA de nopal con el fin de reducir la cantidad de polisacáridos y su influencia en la calidad de la extracción. Posteriormente, se desarrolló un protocolo para la generación de marcadores moleculares del tipo RAPD para nopal y así...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marcadores moleculares; RAPD; Accesión; PCR.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572006000100013

Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation
Provedor de dados: Planta Daninha
Autores: Osuna,M.D.; Goulart,I.C.G.R.; Vidal,R.A.; Kalsing,A.; Ruiz Santaella,J.P.; De Prado,R.
Eleusine indica (goosegrass) is a diploid grass weed which has developed resistance to ACCase inhibitors during the last ten years due to the intensive and frequent use of sethoxydim to control grass weeds in soybean crops in Brazil. Plant dose-response assays confirmed the resistant behaviour of one biotype obtaining high resistance factor values: 143 (fenoxaprop), 126 (haloxyfop), 84 (sethoxydim) to 58 (fluazifop). ACCase in vitro assays indicated a target site resistance as the main cause of reduced susceptibility to ACCase inhibitors. PCR-generated fragments of the ACCase CT domain of the resistant and sensitive reference biotype were sequenced and compared. A point mutation was detected within the triplet of aspartate at the amino acid position 2078...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goosegrass; Cross-resistance; Enzyme; PCR; Herbicide.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582012000300025

Transmissão de begomovírus de plantas daninhas para tomateiros pela mosca-branca
Provedor de dados: Planta Daninha
Autores: Silva,A.K.F.; Santos,C.D.G.; Nascimento,A.K.Q.
Dentre numerosas enfermidades do tomateiro (Lycopersicon esculentum), destacam-se as viroses causadas por begomovírus, os quais são transmitidos pelo vetor Bemisia tabaci biótipo B. Na Chapada da Ibiapaba-CE, os begomovírus têm sido encontrados em várias áreas onde o tomateiro é cultivado, causando sérios danos à produção. Este trabalho teve por objetivos investigar a transmissão de begomovírus a partir de tomateiros infectados para plantas daninhas e verificar seu retorno das plantas daninhas para o tomateiro. Mudas sadias de tomateiro 'Santa Clara' e das plantas daninhas bredo-de-espinho (Amaranthus spinosus), caruru-de-mancha (Amaranthus viridis), mentrasto (Ageratum conyzoides) e picão-preto (Bidens pilosa) foram submetidas à inoculação por dois...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ageratum conyzoides; Amaranthus spinosus; Amaranthus viridis; Bidens pilosa; Lycopersicon esculentum; PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582010000300007

Diversidade genética de Begomovirus que infectam plantas invasoras na região nordeste
Provedor de dados: Planta Daninha
Autores: Asssunção,I.P.; Listik,A.F.; Barros,M.C.S.; Amorin,E.P.R.; Silva,S.J.C.; O. Silva,Izael; Ramalho-Neto,C.E.; Lima,G.S.A.
Os Begomovirus fazem parte de uma família numerosa de fitovírus denominada Geminiviridae. Eles infectam ampla gama de hospedeiras, incluindo muitas espécies cultivadas, como tomate (Lycopersicon esculentum), feijão (Phaseolus vulgaris), pimentão (Capsicum annuum), caupi (Vigna unguiculata), mandioca (Manihot esculenta) etc., além de plantas invasoras de várias espécies. Em alguns casos, plantas invasoras podem funcionar como reservatórios desses vírus para plantas cultivadas, mediante transmissão pelo inseto-vetor. No presente trabalho, plantas invasoras com sintomas de mosaico amarelo, deformação do limbo foliar e redução do crescimento foram avaliadas no tocante à presença de Begomovirus mediante a técnica de PCR, empregando-se oligonucleotídeos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Geminiviridae; Detecção; PCR; Hospedeiras daninhas.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582006000200005

MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPD
Provedor de dados: Planta Daninha
Autores: SILVA,D.R.O.; AGOSTINETTO,D.; VARGAS,L.
ABSTRACT With the increase of glyphosate resistance evolution in hairy fleabane, the knowledge of genetic diversity is essential to assist in the weed management. The objective was to characterize the genetic diversity of glyphosate resistant Conyza species in Rio Grande do Sul. Hairy fleabane biotype seeds were collected in fields of glyphosate-resistant soybean, generating seedlings used for extracting DNA from the leaves to perform a genetic diversity analysis, using the RAPD technique. The DNA was used in polymerase chain reaction and DNA fragments were assessed for polymorphism bands in electrophoresis. Biotypes were identified as Conyza bonariensis and microcephala varieties. In molecular analysis, among 25 primers RAPD, eight were reproducible...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conyza bonariensis; Polymorphism; Glyphosate; PCR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000300433

Laboratory Protocols CIMMYT Applied Molecular Genetics Laboratory
Provedor de dados: Research in Agricultural & Applied Economics (AgEcon Searh)
Autores: Warburton,   Marilyn.
Third Edition
Tipo: Report
Palavras-chave: Chemiluminescence immunoassays; DNA; DNA hybridization; Molecular markers; PCR; RFLPs; Research and Development/Tech Change/Emerging Technologies; F30.
Ano: 2005
URL: http://purl.umn.edu/56184

Laboratory Protocols 2008: Maize Nutrition Quality and Plant Tissue Analysis Laboratory
Provedor de dados: Research in Agricultural & Applied Economics (AgEcon Searh)
Autores: Gallicia,   L.; Nurit,   E.; Rosales,   A.; Palacios-Rojas,   N.
Tipo: Report
Palavras-chave: Zea mays; Maize; Wheats; Triticum; Transgenic plants; Genetic engineering; Gene transfer; Genetic transformation; DNA hybridization; Phenotypes; Selection; Tissue culture; In vitro culture; Biolistics; Research projects; Methods; Molecular cloning; PCR; Crop Production/Industries; F02; F30.
Ano: 2009
URL: http://purl.umn.edu/56177

Protocolos De Laboratorio - Laboratorio De Genética Molecular Aplicada Del CIMMYT
Provedor de dados: Research in Agricultural & Applied Economics (AgEcon Searh)
Tercera Edicion
Tipo: Report
Palavras-chave: Inmunoensayos de quimioluminiscencia; ADN; Hibridación del ADN; Marcadores moleculares; PCR; RAPD; RFLP; Research and Development/Tech Change/Emerging Technologies; F30.
Ano: 2006
URL: http://purl.umn.edu/56183

Diagnosis of Gallibacterium Anatis in Layers: First Report in Turkey
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Yaman,S; Sahan Yapicier,O.
ABSTRACT Gallibacterium anatis, a member of the Pasteurellaceae family, leads to decrease in egg-production, animal welfare and increase in mortality. This study aimed to diagnose G. Anatis, which caused economic losses in laying hens by using conventional and molecular techniques. In this study, G. anatis was examined from a total of 200 dead chicken tissues (heart, liver, lung, spleen and trachea) in laying hen farms that observed a decrease in egg production with respiratory system infection. Conventional methods based on colony morphology, sugar fermentation tests and hemolytic properties and molecular conformation using 16S rRNA-23S rRNA specific primers were performed to identify G. anatis. G. anatis was isolated in 20 (10%) of the examined samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallibacterium anatis; Isolation and identification; Layers; PCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300307

Infectious bronchitis in Brazilian chickens: current data and observations of field service personnel
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Silva,EN.
The infectious bronchitis virus (IBV) was detected for the first time in Brazil by Hipólito in 1957 in chickens sold life in the municipal market of Belo Horizonte, MG, when commercial poultry production was just starting in that country. The Massachusetts (Mass) serotype was identified. However, the clinical disease was only observed in 1975, when poultry production was intensely growing. The extensive outbreak produced the classical condition in layers and breeders, affecting egg production and quality, whereas broilers presented respiratory and "nephritis-nephrosis" signs. The disease rapidly spread to all poultry-producing regions in the country, and in 1979, both the imports and the manufacturing of live vaccines against IB strains Mass, H120 and H52,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Genotyping; Infectious Bronchitis; PCR; Vaccination; Variants.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000300009

Comparative Evaluation of the Effects of Binzalkonium Chloride, Iodine, Gluteraldehyde and Hydrogen Peroxide Disinfectants against Avian Salmonellae Focusing on Genotypic Resistance Pattern of the Salmonellae Serotypes toward Benzalkonium Chloride
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Aksoy,A; El Kahlout,KEM; Yardimci,H.
ABSTRACT Using disinfectants in poultry houses is a common practice to ban the zoonotic pathogens like Salmonella. A major concern in using disinfectants is the emergence of bacteria strains that resist some disinfectants. This phenomenon is manifested in the resistance of some Salmonella serotypes against quaternary ammonium compounds. Such resistance is attributed to qacEΔ1 gene which may be possessed by some Salmonella serotypes. This work aimed to evaluate the resistance of Salmonella serotypes (S. Typhimurium, S. Infantis and S. Entiridis) against different disinfectants (benzalkonium chloride, iodine, gluteraldehyde and hydrogen peroxide). The effect of the disinfectants were evaluated by treatment of the bacteria with different concentrations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disinfectants; PCR; Poultry; Resistance; Salmonella.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100301

Detection of Mycoplasma gallisepticum in dead captive psittacines in Belo Horizonte, Brazil
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Gomes,AM; Costa,LL; Vilela,DAR; Marques,MVR; Carvalhaes,AG; Marin,SY; Costa,MP; Horta,RS; Resende,JS; Martins,NRS.
Mycoplasma gallisepticum (Mg) infection of wild native Brazilian psittacines (Psittaciformes) which died of any cause during sorting, rehabilitation, or conservation, was investigated by PCR. Two previously described PCR methodologies using Mg specific primers were employed for the analyses of 140 swab samples (cloaca, trachea, or palatine cleft). Average positive Mg detection in cloacal swabs was 51.9%, with 80.0% (n=5) of Blue-and-yellow Macaws (Ara ararauna), 60.0% (n=3) Dusky Parrots (Pionus fuscus), 52.5% (n=59) Amazon Parrots (Amazona aestiva), 50.0% (n=2) Orange-winged Parrots (Amazona amazonica), 50.0% (n=2) Jandaya Parakeetsor Jandaya Conures (Aratinga jandaya), 0% (n=2) Golden Conures or Golden Parakeets (Guarouba guarouba), and 0% (n=2) Hyacinth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazona aestiva; Amazona amazonica; Anodorhynchus hyacinthinus; Aratinga jandaya; Brazilian psittacine fauna; Guarouba guarouba; Mycoplasma gallisepticum; PCR; Pionus fuscus; Psittaciformes; Psittacidae.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200001

Salmonella Enteritidis in Layer Farms of Different Sizes Located in Northern China: On-Farm Sampling and Detection by the PCR Method
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Li,X; Liu,L; Li,Q; Xu,G; Zheng,J.
ABSTRACT Salmonella enterica subspecies enterica serotype Enteritidis (SE) has caused foodborne infections over decades. It is transmitted mainly from contaminated eggs to humans. SE is commonly present in layer houses, and closely interacts with environmental factors. The objective of the present study was to develop a viable PCR method to identify SE in environmental samples collected in layer farms of different sizes, and to evaluate SE contamination status in four main egg-production provinces of northern China. After specificity retrieval using Primer-BLAST against the NCBI database, three SE specific oligonucleotide primers were selected as candidate primers. The primers targeting Prot6e gene were adopted and primers targeting Sdf I were also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Environment; Layer farm; PCR; Salmonella Enteritidis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300377

Occurrence of infectious Laryngotracheitis Virus (ILTV) in 2009-2013 in the State of São Paulo - Brazil
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Parra,SHS; Nuñez,LFN; Astolfi-Ferreira,CS; Ferreira,JP.
Infectious laryngotracheitis is a very important respiratory disease because it causes significant economic losses in the poultry industry. The target of ILTV infections is the respiratory system, and the main organ in which the virus remains latent is the trigeminal ganglia. However, the virus has demonstrated tropism for other organs as well. The present study was conducted to determine the presence of Infectious Laryngotracheitis Virus (ILTV) in the state of São Paulo. Samples submitted to LABOR- USP during the last four years (2009-2013) analyzed by a nested/PCR technique. Out of the 682 samples from layers tested for LTIV, 12.46 % were positive, and derived from in both traditional (trachea and trigeminal ganglion) and untraditional (cecal tonsils,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious Laryngotracheitis; PCR; Detection; Trachea; Trigeminal.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000100117

Antimicrobial resistance of bacterial strains isolated from avian cellulitis
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Santos,MM; Alcântara,ACM; Perecmanis,S; Campos,A; Santana,AP.
Avian cellulitis is an inflammatory process in the subcutaneous tissue, mainly located in the abdomen and thighs. This problem is commonly observed in poultry at slaughter and it is considered one of the major causes of condemnation of carcasses in Brazil. The aim of this study was to perform the microbial isolation of lesions of avian cellulitis from a processing plant located in the State of Goiás in order to analyze antimicrobial resistance by antibiogram test and to detect resistance genes by polymerase chain reaction. A total of 25 samples of avian cellulitis lesions were analyzed, from which 30 bacterial strains were isolated. There were eleven (44%) strains of Escherichia coli, nine (36%) strains of Staphylococcus epidermidis, seven (28%) strains of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Antibiotic susceptibility test; Poultry carcasses.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000100002

Detection and molecular characterization of infectious laryngotracheitis virus in laying hens in Brazil
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Villarreal,LYB; Brandão,PEB; Chacón,JLV; Doretto Junior,L; Ito,N; Gama,NS; Ishizuka,MM; Luchese,A; Buchala,F; Astolfi-Ferreira,CS; Ferreira,AJP.
Avian Infectious Laryngotracheitis, caused by Infectious Laryngotracheitis Virus (ILTV), has been reported for decades in Brazilian laying and broiler flocks. More recently, outbreaks have occurred in São Paulo State. This study reports the application of PCR and DNA sequencing targeted to the p32 gene of ILTV using laying chicken samples from Bastos, São Paulo, Brazil. Three out of four field samples were positive by PCR. DNA sequencing of two samples evidenced homology of the amplified fragments with the p32 gene of ILTV. The results definitely confirmed the presence of ILTV in the birds during the outbreak. Further studies are needed to establish the sources of infection and to determine whether the detected virus was originated from vaccine or field...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian infectious laryngotracheitis; Brazil; P32; PCR; Sequencing.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000400011

Test profiles of broiler breeder flocks housed in farms with endemic Mycoplasma synoviae infection
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Fiorentin,L; Mores,MAZ; Trevisol,IM; Antunes,SC; Costa,JLA; Soncini,RA; Vieira,ND.
There is a need for a better understanding of the epidemiology of Mycoplasma synoviae (MS) infection in broiler breeders in Brazil. Many features of the infection remain unrecognizable, because there are no clinical signs of the disease. A detailed testing was performed at each 6 to 8 weeks in three MS-free flocks introduced in farms with endemic MS infection for a follow-up epidemiological study. Every flock was monitored by polymerase chain reaction (PCR), by serum plate agglutination (SPA) and hemagglutination inhibition (HI) for serology studies, and isolation of mycoplasmas from tracheal swabs. PCR was found to be the most sensitive test, detecting early MS infection. Serology was positive in less than 50% of the sera and MS was isolated only between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler breeders; Mycoplasma synoviae; PCR; Serology; Survey; Transmission.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000100005

Molecular Diagnostic of Chicken Parvovirus (ChPV) Affecting Broiler Flocks in Ecuador
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Torre,D De la; Nuñez,LFN; Puga,B; Parra,SHS; Astolfi-Ferreira,CS; Ferreira,AJP.
ABSTRACT Enteric diseases affect poultry and cause important economic losses in many countries worldwide. Avian parvovirus has been linked to enteric conditions, such as malabsorption and runting-stunting syndrome (RSS), characterized by diarrhoea, and reduced weight gain and growth retardation. In 2013 and 2016, 79 samples were collected from different organs of chickens in Ecuador that exhibited signs of diarrhea and stunting syndrome, and analysed for the presence of chicken parvovirus (ChPV). The detection method of ChPV applied was Polymerase Chain Reaction (PCR), using primers designed from the conserved region of the viral genome that encodes the non-structural protein NS1. Out of the 79 samples, 50.6% (40/79) were positive for ChPV, and their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken parvovirus; Enteric diseases; Molecular diagnostic; PCR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400643

Molecular analysis of the bacterial diversity in a specialized consortium for diesel oil degradation
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Paixão,Douglas Antonio Alvaredo; Dimitrov,Mauricio Rocha; Pereira,Rodrigo Matheus; Accorsini,Fábio Raphael; Vidotti,Maria Benincasa; Lemos,Eliana Gertrudes de Macedo.
Diesel oil is a compound derived from petroleum, consisting primarily of hydrocarbons. Poor conditions in transportation and storage of this product can contribute significantly to accidental spills causing serious ecological problems in soil and water and affecting the diversity of the microbial environment. The cloning and sequencing of the 16S rRNA gene is one of the molecular techniques that allows estimation and comparison of the microbial diversity in different environmental samples. The aim of this work was to estimate the diversity of microorganisms from the Bacteria domain in a consortium specialized in diesel oil degradation through partial sequencing of the 16S rRNA gene. After the extraction of DNA metagenomics, the material was amplified by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Cloning; 16S rRNA; Metagenomics; Soil; Biosurfactants.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000300019

Diversidade genética e eficiência simbiótica de rizóbios noduladores de acácia-negra de solos do Rio Grande do Sul
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Vargas,Luciano Kayser; Lisboa,Bruno Brito; Scholles,Dércio; Silveira,José Ricardo Pfeifer; Jung,Gabriela Cardoso; Granada,Camille Eichelberger; Neves,Andrei Gibbon; Braga,Marcos Martins; Negreiros,Tatiana.
A acácia-negra é a terceira espécie florestal mais cultivada no Brasil. Além de sua importância econômica, é utilizada na recuperação de áreas degradadas, nas quais o solo geralmente apresenta pH baixo e altos teores de Al. O presente trabalho objetivou avaliar a diversidade genética de rizóbios naturais de solos do Rio Grande do Sul e selecionar isolados eficientes na fixação de N2 em condições de pH baixo. Um total de 50 isolados de Bradyrhizobium sp. foi obtido, os quais, juntamente com as estirpes recomendadas BR 3067 e BR 3068, foram caracterizados com o marcador BOX A 1-R. O padrão de bandas dos isolados foi utilizado na construção de um dendrograma, a partir do qual se calculou o índice de diversidade de Shannon. Dez isolados foram testados quanto à...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fixação biológica de nitrogênio; PCR; Rhizobium; Acacia mearnsii.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832007000400005

Caracterização do dna ribossômico do isolado de Scleroderma UFSMSc1 de Eucalyptus grandis W. Hill ex-maiden
Provedor de dados: Revista Brasileira de Ciência do Solo
Autores: Lupatini,Manoeli; Mello,Andrea Hentz de; Antoniolli,Zaida Inês.
O ácido desoxiribunocléico ribossomal (rDNA) é utilizado como uma ferramenta importante para caracterizar o polimorfismo entre os fungos. Existem muitas cópias de rDNA as quais são arranjadas por espaços não codificados. Essas cópias são altamente conservadas entre espécies de fungos. O objetivo deste trabalho foi estudar a região do Espaço Interno Transcrito (ITS) e analisar as diferenças no polimorfismo da seqüência dessa região no fungo Scleroderma UFSMSc1 com seqüências dos isolados de Scleroderma e Pisolithus do banco de dados GenBank. O DNA do isolado de Scleroderma UFSMSc1 foi extraído por meio da solução de extração à base de CTAB. A partir do DNA, foram feitas reações de PCR com os oligonucleotídeos iniciadores universais ITS1 e ITS4, cujo produto...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caracterização do DNA; PCR; Scleroderma.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832008000700010

Detecção de Begomovirus em maracujazeiro (Passiflora edulis Sims) no Estado de Alagoas
Provedor de dados: Revista Brasileira de Fruticultura
Autores: Silva,Sarah Cavalcanti da; Assunção,Iraildes Pereira; Carnaúba,Juliana Paiva; Lima,Joyce Silva; Amorim,Edna Peixoto da Rocha; Lima,Gaus de Andrade.
O maracujazeiro é uma cultura de grande importância econômica nos países tropicais, destacando-se o Brasil como o maior produtor. No presente trabalho, é relatada a infecção de plantas de maracujazeiro por um vírus do gênero Begomovirus, família Geminiviridae, no município de Anadia, Estado de Alagoas. As plantas infectadas apresentavam sintomas de mosaico amarelo, redução drástica de crescimento e da área foliar. DNA extraído de plantas sintomáticas foi empregado como molde em PCRs, contendo os pares de primers PAL1v1978 e par1C496 ou PBL1v2040 e PCRc1 que direcionam, respectivamente, a amplificação de regiões de aproximadamente 1,2 kb do DNA-A e 0,6 kb do DNA-B, do genoma dos begomovírus. Fragmentos de tamanho esperado foram amplificados a partir de...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Geminividae; Diagnose; PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452006000300037

AVALIAÇÃO DE PLANTAS MATRIZES DE ABACAXIZEIRO CULTIVAR SMOOTH CAYENNE UTILIZANDO MARCADORES RAPD E PADRÕES ISOENZIMÁTICOS
Provedor de dados: Revista Brasileira de Fruticultura
Autores: GOTTARDI,MARIA VITÓRIA CECCHETTI; LEMOS,ELIANA GERTRUDES MACEDO; RUGGIERO,CARLOS.
Foram coletadas, em área comercial da fazenda Córrego dos Bois, município de Canápolis -- MG, 20 plantas matrizes de abacaxizeiro cultivar Smooth Cayenne, para avaliação de similaridade e padrões genotípicos através de marcadores moleculares RAPD e padrões isoenzimáticos. As plantas matrizes foram selecionadas mediante as seguintes características: planta sadia, frutos cilíndricos, ausência de fasciação, pedúnculo curto, ausência de espinhos nas folhas, "olho" do fruto chato e peso dos frutos entre 1,6 a 2,2kg. Para análise de marcadores RAPD, foram testados 100 "primers", dos quais, 43 foram eficientes na amplificação das amostras, onde foram observados padrões de bandas diferentes entre as plantas matrizes utilizadas, indicando a existência de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus; PCR; Polimorfismo; Variabilidade genética.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452001000300002

Avaliação por RAPD de plantas de abacaxizeiro cultivar Smooth Cayenne derivadas do seccionamento do talo e cultura de tecidos
Provedor de dados: Revista Brasileira de Fruticultura
Autores: GOTTARDI,MARIA VITÓRIA CECCHETTI; LEMOS,ELIANA GERTRUDES MACEDO; RUGGIERO,CARLOS.
Foram coletadas, em área comercial da fazenda Córrego dos Bois, município de Canápolis -- MG, plantas de abacaxizeiro cultivar Smooth Cayenne, para serem avaliadas quanto à propagação pelo método do seccionamento do talo e cultura de tecidos, bem como análise por RAPD das mudas decorrentes destes dois processos de propagação. A propagação pelo seccionamento do talo foi eficiente na produção de mudas, tanto em quantidade como em qualidade, em um curto espaço de tempo, além de apresentar a mesma característica genotípica (análise por RAPD) das plantas-matrizes de origem. Já no processo de produção de mudas por cultura de tecidos, não foi obtida uma quantidade suficiente de mudas que comprovasse a utilização de uma metodologia mais sofisticada. Além da perda...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus; PCR; Polimorfismo; Variabilidade genética.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000100002

Caracterização molecular dos alelos-S de incompatibilidade gametofítica em Prunus salicina Lindl.
Provedor de dados: Revista Brasileira de Fruticultura
Autores: Mota,Monalize Salete; Bianchi,Valmor João; Carvalho,Alexandre Zanardo de; Braga,Eugenia Jacira Bolacel; Peters,José Antônio.
Pomares comerciais de ameixeira-japonesa devem conter pelo menos duas cultivares para obter boa fertilização, devido à presença do sistema de incompatibilidade gametofítica, que inibe a autofecundação da grande maioria das cultivares. No presente trabalho, buscou-se identificar e caracterizar molecularmente os alelos-S de 11 cultivares de ameixeira-japonesa (Prunus salicina Lindl.) e verificar a compatibilidade entre os genótipos avaliados. As cultivares Santa Rosa, Santa Rita, Reubennel, Pluma 7, América, Rosa Mineira, Amarelinha, The First, Gulfblaze (Clone São Paulo), Gulfblaze (Clone Guaiba) e Harry Pickstone foram analisadas por meio de Reação em Cadeia da Polimerase (PCR) com três pares de iniciadores específicos para alelos-S. As condições da PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ameixeira-japonesa; Autoincompatibilidade gametofítica; PCR; Alelos-S.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000300020

Análise de RAPD na identificação de cultivares: uma metodologia útil?
Provedor de dados: Revista Brasileira de Sementes
Autores: Binneck,Eliseu; Nedel,Jorge Luiz; Dellagostin,Odir A.
Caracteres morfológicos têm sido utilizados tradicionalmente como assinaturas da identidade e pureza varietal e genética. Esses descritores se constituem em uma base pobre, por ser uma medida indireta da composição genética do material. Uma vez que caracteres moleculares revelam diferenças genéticas mais rapidamente, com maior precisão e sem o obscurecimento causado pelo efeito ambiental, oferecem vantagens significantes em termos de discriminação, confiabilidade, rapidez e custo reduzido. Um método molecular relativamente novo, DNA polimórfico amplificado ao acaso (RAPD), baseado na reação em cadeia da polimerase (PCR), tem sido adotado por alguns pesquisadores envolvidos no desenvolvimento de métodos de identificação de cultivares principalmente por ser...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA polimórfico amplificado ao acaso (RAPD); Reprodutibilidade; Marcadores moleculares; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222002000100027

Efeito do gene do estresse suíno sobre características de quantidade e qualidade de carcaça
Provedor de dados: Revista Brasileira de Zootecnia
Autores: Bastos,Reginaldo Gaspar; Federizzi,Joreci; Deschamps,João Carlos; Cardellino,Ricardo Alberto; Dellagostin,Odir Antonio.
O gene do estresse suíno (gene hal), em homozigose recessiva (nn), está associado com a ocorrência da Síndrome do Estresse Porcino (PSS) e com a ocorrência da carne pálida, mole e exudativa (PSE). Em heterozigose (Nn), está relacionado com diminuição na qualidade da carne porém, com maior peso de carcaça. Neste estudo, foi caracterizado o genótipo do gene hal de 160 suínos por meio de análise do DNA extraído de um único folículo piloso. Estes animais foram abatidos e, após, mensuradas características de carcaça de cada um. Entre os 160 animais analisados, 82 (52,58%) foram caracterizados como NN, 67 (41,80%) como Nn e nove (5,62%) como nn. A variação das características de carcaça entre os genótipos foi avaliada por análise de variância, usando o SAS. Os...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carcaça suína; Gene halotano; PCR; PSE; PSS.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982001000100007

Neospora caninum: Detección de ADN en sangre durante la primera gestación de vaquillas infectadas naturalmente
Provedor de dados: Revista Veterinaria México
Autores: Santana,Omar Iván; Cruz-Vázquez,Carlos; Medina-Esparza,Leticia; Ramos Parra,Miguel; Castellanos Morales,Ciro; Quezada Gallardo,Daniel.
El objetivo del trabajo fue detectar la presencia de ADN de N. caninum en animales infectados naturalmente, en dos tiempos de su primera gestación y al parto, así como registrar la presentación de abortos. Se seleccionó, en un establo, con presencia de parasitosis, un lote de 20 hembras de entre 12 y 14 meses de edad, seropositivas en ELISA, las hembras fueron inseminadas artificialmente y se tomaron muestras de sangre en el primero y segundo tercios de gestación y al parto; se aisló ADN y se sometió a PCR anidado en un solo tubo con iniciadores específicos. En el muestreo correspondiente al primer tercio de la gestación, se observaron 7/20 casos positivos (35%), en el segundo 15/20 (75%) y al parto 10/20 casos positivos (50%). De los animales incluidos en...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neospora caninum; ADN; Sangre; PCR; Ganado lechero; México.
Ano: 2010
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922010000200006

Caracterización de los tipos capsulares de Pasteurella multocida en exudado faríngeo de bovinos productores de carne clínicamente sanos en el estado de Querétaro
Provedor de dados: Revista Veterinaria México
Autores: Villegas Vázquez,Ana Lilia; Campuzano-Ocampo,Víctor Manuel; Hernández-Castro,Rigoberto; Suárez-Güemes,Francisco; Trigo Tavera,Francisco José; Jaramillo-Arango,Carlos Julio.
El objetivo del presente estudio fue identificar y caracterizar los tipos capsulares de P. multocida en exudado faríngeo en bovinos destinados a la producción de carne en el estado de Querétaro. Se obtuvieron, mediante hisopo, 227 muestras de exudado faríngeo de animales clínicamente sanos en una planta de sacrificio ubicada en el municipio de Ezequiel Montes, Querétaro. Las muestras se sembraron en agar sangre y se incubaron a 37°C por 24 h en aerobiosis. Las cepas aisladas fueron identificadas mediante características morfológicas, pruebas bioquímicas convencionales y el microsistema comercial API 20NE. La tipificación de los grupos capsulares A y D se realizó por medio de una PCR múltiple para la amplificación de los genes hyaD-hyaC y dcbF,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pasteurella multocida; Tipos capsulares; Caracterización bioquímica; Caracterización molecular; Exudado faríngeo; Bovinos; PCR.
Ano: 2014
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922014000200003

Caracterización fenotípica y molecular de cepas de Pasteurella multocida aisladas de exudado nasal de bovinos, en dos cuencas lecheras de México
Provedor de dados: Revista Veterinaria México
Autores: Campuzano Ocampo,Víctor Manuel; González Rodríguez,Alma Delia; Hernández Castro,Rigoberto; Suárez Güemes,Francisco; Trigo Tavera,Francisco José; Jaramillo Arango,Carlos Julio.
Se obtuvieron 250 cepas de P. multocida aisladas de exudado nasal, 182 cepas de bovinos clínicamente sanos y 68 cepas de bovinos clínicamente enfermos de neumonía, de dos complejos lecheros, uno en la región de Tizayuca estado de Hidalgo (n = 81), y otro en la Región Lagunera de los estados de Coahuila y Durango (n = 169), México. Las cepas fueron identificadas mediante pruebas bioquímicas convencionales y el sistema comercial API 20NE. La tipificación capsular se realizó por medio de las pruebas de hiauloronidasa y acrifavina, así como por medio de una PCR múltiple para la amplificación de los genes hyaD-hyaC y dcbF. Los resultados globales mediante la prueba de hialuronidasa mostraron que 90.4% (226/250) de las cepas fueron del tipo capsular A y por...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pasterurella multocida; Tipos capsulares; Caracterización fenotípica; Caracterización molecular; Exudado nasal; Bovinos; PCR.
Ano: 2011
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922011000100001

Diagnóstico molecular e parasitológico de Ehrlichia Canis em cães no município de Ilhéus-BA
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Rocha, Josiane Moreira; Carvalho, Fabio Santos; Oliveira, Haniel Cedraz de; Munhoz, Alexandre Dias; Wenceslau, Amauri Arias.
Objetivou-se diagnosticar Erlichia canis com o emprego de dois métodos: i) molecular (PCR) e; ii) parasitológico (imprint em lâmina). Para tanto, foram selecionados cães com alterações clínicas, hematológicas e bioquímicas (febre, petéquias, alterações de linfonodos periféricos, apatia, trombocitopenia, anemia e pancitopenia e presença de carrapatos). Foram coletadas amostras de 10ml de sangue de 287 cães de seis bairros do município de Ilhéus, Bahia. Foi realizado o esfregaço de sangue de ponta de orelha em lâminas coradas por kit Panótico rápido, hemograma completo, realizado em contador automático ABCVet (Animal Blood Counter) utilizando kit ABX Vetpack (HURIBA). Parte do sangue foi centrifugado para obtenção do soro e verificação dos níveis de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erliquiose; PCR; Cão.
Ano: 2013
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/21342

Análise comparativa da PCR e HA no diagnóstico de cães suspeitos de parvovirose
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Dantas, Liliane de Oliveira; Garcia, Rita de Cássia N. Cubel; Miranda, Suzana Carvalho de; Haanwinckel, Maria Cristina Santos; Menini, Rodrigo; Megid, Jane.
O presente estudo teve por objetivo diagnosticar a parvovirose em cães clinicamente suspeitos, utilizando-se a reação em cadeia pela polimerase (PCR) e hemaglutinação (HA) para identificação do agente. Foram examinadas 38 amostras fecais de cães SRD, de ambos os sexos, com idade entre três a sete meses, atendidos no hospital veterinário da FMVZ-UNESP/Botucatu, apresentando suspeita clínica de parvovirose, em presença de quadro de diarreia sanguinolenta, êmese, anorexia, apatia, hipertermia e leucopenia. As amostras fecais foram coletadas por meio de sonda, armazenadas em eppendorfs e congeladas até o processamento. Entre as amostras examinadas, 23 foram positivas para PCR e 21 positivas para HA, independente do animal ter ou não recebido pelo menos uma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cães; Parvovirose; PCR; HA.
Ano: 2013
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/18635

Diagnóstico de leishmaniose visceral canina em animais do Centro de Controle de Zoonoses da Bahia
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Carvalho, Fábio Santos; Rocha, Josiane Moreira; Wenceslau, Amauri Arias; Oliveira, Haniel Cedraz de; Santos, Ivanildo dos Anjos; Gross, Eduardo.
A Bahia é o estado da região Nordeste com maior número de registros de leishmaniose em humanos.Os cães também podem se infectar e tem importante papel na manutenção da doença no ambiente urbano. Sendo assim, objetivou-se investigar a ocorrência de leishmaniose visceral canina em animais das unidades de Centro de Controle de Zoonoses (CCZ) da Bahia utilizando técnicas sorológicas e moleculares. Foram examinados 100 cães dos CCZ dos municípios Barreiras, Eunápolis, Ilhéus, Itabuna e Feira de Santana. Após exame clínico dos animais coletou-se 10 mL de sangue da veia jugular para avaliação hematológica, extração de DNA e obtenção de soro. O DNA genômico foi extraído utilizando-se fenol:clorofórmio:álcool isoamilico (25:24:1). O diagnóstico sorológico foi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L. infantum; ELISA; PCR; Cães.
Ano: 2015
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/25304

Detecção da infecção pelo vírus da anemia infecciosa equina (EIAV): IDGA, NESTED-PCR E RT-PCR
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Cruz, Antonio Joselito Oliveira; Brandão, Camila Fonseca Lopes; Campos, Gubio Soares; Tigre, Dellane Martins; Sardi, Silvia Ines.
A Anemia Infecciosa Equina (AIE) é uma doença infecciosa viral que atinge todos os membros da família Equidae. A AIE é causada pelo Vírus da Anemia Infecciosa Equina (EIAV), um vírus RNA de fita dupla, pertencente à família Retrovirídae e gênero Lentivirus. As doenças causadas pelos retrovírus apresentam- se como infecções persistentes, com período de latência que pode se estender por toda a vida do animal, dificultando assim o diagnóstico da infecção. A Imunodifusão em Gel de Agarose (IDGA) é o teste sorológico mais empregado e oficialmente reconhecido para o EIAV em vários países, porém exige altos títulos de anticorpos para a reação antígeno–anticorpo, visualizado a olho nu pela formação de linhas de precipitação no gel. A soroconversão do animal pode...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AIE; PCR; IDGA AIE; PCR; IDGA.
Ano: 2013
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/21334

Saúde e produção de ruminantes e equídeos P-187 Detecção molecular de Ehrlichia spp. em animais domésticos do pantanal matogrossense
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Ziliani, Thaysa Felfili; Aguiar, Daniel Moura de; Melo, Andréia Lima Tomé; Azevedo, Jaqueline Bruning; Figueiredo, Thaiza Cristina Fonseca de.
O gênero Ehrlichia agrupa patógenos de importância em medicina veterinária e saúde pública. Até 2011, quatro espécies do gênero Ehrlichia haviam sido relatadas no Brasil por isolamento e PCR:Ehrlichia canis, E. ewingii e E. chaffeensis além de uma espécie filogeneticamente próxima à E. ruminantium. Em 2012, descreveu-se o isolamento da espécie E. mineirensis em Minas Gerais. O presente trabalho procurou obter novas informações sobre a epidemiologia das erliquioses em Mato Grosso; propondo a identificação e caracterização genética e ocorrência de espécies do gênero Ehrlichia a partir de sangue de bovinos, equinos e ovinos pela amplificação parcial do gene dsb de Ehrlichia pela Reação em Cadeia pela Polimerase em dois tempos (hnestedPCR). Foram efetuadas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erliquiose; PCR; DSB.
Ano: 2014
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/23863

Saúde e produção de ruminantes e equídeos P-191 Diagnóstico de patógenos envolvidos na mastite subclínica em vacas utilizando as técnicas convencional e molecular
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Farias, Elaine Cristina; Carvalho, Fábio Santos; Oliveira, Haniel Cedraz de; Amorim, Lucimar Souza; Costa, Sonia Carmen Lopo; Wenceslau, Amauri Arias.
A mastite é a doença infecciosa mais comum que afeta vacas leiteiras, sendo a sua forma a subclínica mais prevalente (70%). Testes como California Mastitis Test (CMT) são recomendados para detectar animais positivos, pois essa forma da doença apresenta-se assintomática no animal. Objetivou-se com este estudo realizar um levantamento de casos de mastite subclínica no município de Ibicaraí-Ba. Foi realizada a identificação de vacas positivas ao teste CMT com posterior caracterização dos patógenos pela técnica da PCR (Reação em Cadeia da Polimerase), utilizando-se primers específicos. Foram analisadas 112 vacas em lactação pelo teste CMT.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CMT; Leite bovino; Infecção; PCR.
Ano: 2014
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/23855

Leptospirose em ovinos mestiços nativos abatidos em uma região semiárida do Brasil
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Costa, D. F.; Silva, A. F.; Brasil, A. W. L.; Loureiro, A. P.; Santos, F. A.; Azevedo, S. S.; Lilenbaum, W.; Alves, C.
A criação de ovinos é uma atividade econômica de grande valor para a indústria pecuária brasileira. As doenças infecciosas podem comprometer significativamente a sua produtividade. O objetivo deste estudo foi detectar Leptospira spp. em ovinos sem raça definida a partir de uma região semiárida do Estado da Paraíba, Nordeste do Brasil. Quarenta e nove amostras de sangue e tecido renal, bem como amostras de urina de 25 (49 ovelhas) foram coletadas na linha de abate. A presença de anticorpos antiLeptospira spp. foi verificada com o teste de aglutinação microscópica (SAM). As amostras de urina e rins foram processadas por PCR e cultura bacteriológica. Sororreatividade foi observada em 8,2% (4/49) dos animais. Em relação à PCR, três amostras renais foram...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolamento; Leptospirose; Matadouro; Ruminantes; PCR; Sorologia.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/32183

Ocorrência de dna leptospírico em fluído vaginal de bovinos
Provedor de dados: Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP
Autores: Oliveira, Fernanda Santana; Medeiros, Marcos Alberto; Hanmond, Camila; Pinna, Melissa Hanzen; Lilenbaum, Walter.
A leptospirose é uma doença cosmopolita de ocorrência frequente em bovinos, é considerada uma importante zoonose, sendo responsável por significativas perdas econômicas para a produção pecuária. As manifestações clínicas apresentadas por um animal com leptospirose são infertilidade, abortamentos, diminuição da produção de leite e natimortalidade. O presente estudo teve por objetivo a detecção de DNA leptospírico em 67 amostras de fluido vaginal de bovinos abatidos no Rio de Janeiro-Brasil. A coleta foi realizada por meio de swab estéril, coletado diretamente do assoalho da vagina, armazenada em tubo Falcon de 15ml contendo 2ml de solução tampão até a chegada ao laboratório de Bacteriologia Veterinária da Universidade Federal Fluminense. Posteriormente as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirose; Bovinos; PCR.
Ano: 2013
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/22133

Genetic variability of HIV-1 isolates from Minas Gerais, Brazil
Provedor de dados: Revista de Microbiologia
Autores: Proietti,Anna Bárbara de Freitas Carneiro; Barbosa,Edel Figueiredo; Silva,Janaína Guernica; Carvalho,Alex Fiorini de; Kroon,Erna Geessien; Ferreira,Paulo César Peregrino.
We report results of nucleotide sequencing and phylogenetic analysis of the env gene of 11 HIV-1 isolates, in Belo Horizonte, Brazil. Ten isolates belonged to HIV-1 subtype B and one was a probable B/F mosaic. This putative B/F recombinant is similar but not identical in its nucleotide sequence to other B/F mosaics described in Brazil.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HIV-1; Genome; PCR; AIDS; Hemophiliacs.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200010

Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction
Provedor de dados: Revista de Microbiologia
Autores: Sircili,Marcelo Palma; Roxo,Eliana; Leão,Sylvia Cardoso.
Mycobacterium avium complex (MAC) species cannot be discriminated by the usual methods of biochemical identification of mycobacteria. This study showed that amplification by PCR of DT1 and DT6, two single copy sequences identified in the genome of M. avium serotype 2, the insertion sequence IS1245, found to be consistently present in M. avium strains and the heat-shock protein gene hsp65, followed by restriction polymorphism analysis, are rapid and accurate tests for the differentiation of the species M. avium, M. intracellulare, and M. scrofulaceum.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Amplification; PCR; MAC; Mycobacterium; Identification.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200011

Diversidad molecular de begomovirus en el cultivo del pimiento (Capsicum annuum L.) en Cuba
Provedor de dados: Revista de Protección Vegetal
Autores: Quiñones Pantoja,Madelaine; Castillo Urquiza,Gloria; Alfenas Zerbini,Poliane; Murilo Zerbini,Francisco.
El objetivo de este trabajo fue determinar la diversidad molecular de begomovirus asociados al cultivo del pimiento en Cuba. Para ello, en el periodo 2006- 2010 se realizó una prospección de begomovirus en las principales áreas de producción de pimiento en Cuba. Se colectaron 600 muestras foliares de plantas que mostraron síntomas típicos de enfermedades virales y de estas se seleccionaron al azar 50 por región para su evaluación. El ADN total extraído se evaluó mediante una PCR con cebadores universales a begomovirus (Palv1978/PARc496). A partir de muestras positivas se amplificó su ADN genómico circular completo mediante la amplificación en círculo rodante. Los productos se caracterizaron mediante análisis de restricción, utilizando las enzimas Sac I,...
Tipo: Journal article
Palavras-chave: Begomovirus; PCR; Amplificación en Círculo Rodante; Polimorfismo de longitud de fragmentos de restricción; Diversidad molecular.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000100007

AISLAMIENTO E IDENTIFICACIÓN MORFOLÓGICA, BIOQUÍMICA Y MOLECULAR DE Leifsonia xyli subsp. xyli EN CUBA
Provedor de dados: Revista de Protección Vegetal
Autores: Iglesia,Aleika; González,Raiza; Martín,Dayamí; Díaz,Maricela; Álvarez,Elba.
En este trabajo se obtiene el cultivo puro de Leifsonia xyli subsp. xyli a partir de variedades cubanas de caña de azúcar y se logra disminuir las dificultades presentadas hasta el momento con el aislamiento de esta fitobacteria de naturaleza fastidiosa. Se evidencia el pleomorfismo típico de esta especie bacteriana y la total coincidencia de las características bioquímicas y moleculares que la identifican. Se cuenta por primera vez en Cuba con aislados caracterizados que pueden ser empleados en futuras investigaciones y servicios, así como en la obtención de antisueros para el diagnóstico serológico de esta importante enfermedad.
Tipo: Journal article
Palavras-chave: Leifsonia xyli subp. xyli; Morfología; Bioquímica; PCR; Identificación.
Ano: 2007
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522007000100004

Coexistencia de potyvirus y begomovirus en el cultivo del pimiento (Capsicum annuum L.) en Cuba
Provedor de dados: Revista de Protección Vegetal
Autores: Quiñones,Madelaine; Martínez,Yamila; Arana,F; Martínez,María de los A; Zamora,Loidy; Miranda,Ileana; Zerbini,F.M.
El objetivo del trabajo fue determinar la distribución de potyvirus y begomovirus en áreas productoras de pimiento (Capsicum annuum L.) en Cuba. Se colectaron 600 muestras con síntomas virales durante el periodo 2006-2010. Las muestras se evaluaron mediante el método UMELISA-DAS utilizando un anticuerpo policlonal a PVY para detectar su presencia y de estas se evaluaron 50 plantas positivas con los cebadores universales (PolyT/Poty 4) en una RT-PCR para confirmar el resultado. También se extrajo el ADN total de las plantas y se analizó mediante la técnica de HAN no radiactiva con una sonda de la región intergénica del TYLCV, cuyos resultados se confirmaron en 50 muestras positivas utilizando una PCR con cebadores genéricos (Palv1978/PARc496). La evaluación...
Tipo: Journal article
Palavras-chave: PVY; TYLCV; UMELISA-DAS; PCR; HAN.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522013000100005

DESCUBRIENDO LA ETIOLOGÍA DE ENFERMEDADES DE LA FRUTA BOMBA EN CUBA, REPÚBLICA DEMOCRÁTICA DEL CONGO (DRC) y ETIOPÍA
Provedor de dados: Revista de Protección Vegetal
Autores: Arocha,Y; Almeida,R; Vigheri,N; Nkoy-Florent,; Betts,P; Monger,W. A.; Harju,V; Mumford,R.A; Bekele,B; Tadesse8,Dereje; Jones,P.
La fruta bomba se encuentra entre los frutales más comunes que se cultivan en Etiopía. Está siendo afectada por un desorden tipo declinamiento, recientemente informado en las principales parcelas, y causando pérdidas superiores al 30%. En North Kivu, DRC, es el principal cultivo de supervivencia, cultivado para la obtención de látex que se exporta a Europa, para la producción de papaína. Desde 1997, la enfermedad se ha expandido gradualmente, afectando seriamente los niveles de látex. En Cuba, la fruta bomba se ha convertido en uno de los más importantes cultivos de exportación, principalmente para los mercados canadiense y europeo. Recientemente, la enfermedad similar al cogollo arrepollado, papaya bunchy top like-disease (PBT-like) ha sido asociada con...
Tipo: Journal article
Palavras-chave: Fruta bomba; Fitoplasmas; Virus de la mancha anular; Potyvirus; Virus del mosaico del melón de Marruecos; Ricketsias; PCR.
Ano: 2008
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522008000100004

CARACTERIZACIÓN MOLECULAR DE AISLADOS DE CAMPO DEL VIRUS DEL ENCRESPAMIENTO AMARILLO DE LA HOJA DEL TOMATE (TYLCV)
Provedor de dados: Revista de Protección Vegetal
Autores: Quiñones,Madelaine; Fonseca,D; Martínez,Yamila.
Los begomovirus constituyen «virus emergentes» de plantas. Debido a las infecciones persistentes en los campos de tomate, las pérdidas ocasionadas por estos virus a la agricultura mundial y a la gran variabilidad informada para muchos de sus miembros, por lo que el objetivo de este trabajo fue caracterizar la variabilidad genética de la población natural del TYLCV predominante en Cuba. Para esto, se secuenciaron 21 aislados de campo del TYLCV, obtenidos de diferentes años y regiones del país. Se utilizaron cebadores específicos para la amplificación y secuenciación de la región intergénica (RI) y del extremo 5` terminal del gen c1 o proteína de la replicación (rep). Las secuencias obtenidas se analizaron mediante programas bioinformáticos para determinar...
Tipo: Journal article
Palavras-chave: TYLCV; Begomovirus; Tomate; Variabilidad genética; PCR; Secuenciación; Estructura secundaria.
Ano: 2007
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522007000100007

Uso del microinjerto in vitro de ápices caulinares para eliminar ´Candidatus Liberibacter asiaticus´ en cultivares de cítricos en Cuba
Provedor de dados: Revista de Protección Vegetal
Autores: Zamora-Rodríguez,Victoria; Luís-Pantoja,Maritza; Peña-Bárzaga,Inés; Ferriol-Marchena,Xenia; Hernández-Rodríguez,Lester.
El objetivo del estudio fue optimizar la técnica de microinjerto in vitro de ápices caulinares (MIV) para eliminar Candidatus Liberibacter asiaticus (CLas) en cultivares de cítricos infectados. Como fuentes de brotes se emplearon plantas de cinco cultivares con síntomas de huanglongbing (HLB): mandarinos Satsuma Tropical y Parson's Special, los híbridos de mandarinos Sunburst IVIA 200-C-1 y tangelo Nova IVIA 74-7 y limero Persa SRA-58. Para estandarizar el tamaño de ápice, suficiente para la eliminación de CLas, se ensayaron tres dimensiones compuestas por el meristemo apical con 2, 3 y 4 primordios foliares (pf) (0,1 - 0,4 mm). La presencia de CLas se constató mediante PCR dúplex en las plantas sintomáticas y PCR anidada en las plantas obtenidas por MIV....
Tipo: Journal article
Palavras-chave: Microinjerto in vitro; Saneamiento; Huanglongbing; PCR; NPCR.
Ano: 2015
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522015000200006

DIAGNÓSTICO MOLECULAR DE LA MARCHITEZ BACTERIANA
Provedor de dados: Revista de Protección Vegetal
Autores: Iglesia,Aleika; Alvarez,Elba; Martínez,Yamila; García,A.
La marchitez bacteriana causada por Ralstonia solanacearum, es una de las enfermedades más importante que afecta a una amplia gama de cultivos en las regiones tropicales y subtropicales. En Cuba esta enfermedad se encuentra bajo estrictas medidas de cuarentena, sin embargo el riesgo de entrada crece cada año debido a la importación de material vegetal procedente de zonas de altos niveles de infestación. En este trabajo se introduce y desarrolla el uso de la reacción en cadena de la polimerasa (PCR) para la detección y confirmación de R. solanacearum en materiales importados, así como en la vigilancia de la enfermedad en el sistema nacional de sanidad vegetal. La metodología utilizada facilitó la detección de R. solanacearum en cultivos de células hasta...
Tipo: Journal article
Palavras-chave: Cuarentena; Diagnóstico molecular; Ralstonia solanacearum; PCR.
Ano: 2008
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522008000200002

EXPRESIÓN in vitro EN Escherichia coli, DE UN FRAGMENTO DEL GEN vlhA.5.02 DE LA PRINCIPAL FAMILIA DE HEMAGLUTININAS DE Mycoplasma gallisepticum
Provedor de dados: Revista de Salud Animal
Autores: Agüero,J.A; Hotzel,H; Aguilar,Lisandra; Lima,Claudia; Sachse,K; Martínez,Siomara.
Mycoplasma gallisepticum es el agente etiológico fundamental del síndrome respiratorio crónico de los pollos; constituye el micoplasma más importante desde el punto de vista económico para la avicultura. Un fragmento de ADN de aproximadamente 800pb de este microorganismo, amplificado mediante la reacción en cadena de la polimerasa a partir del plásmido pTTQ18 recombinante, se clonó en el vector de expresión pQE-32. El polipéptido se expresó fusionado a una secuencia de seis histidinas, por lo que la talla final fue de 31kDa, y se detectó mediante inmunotransferencia con un antisuero policlonal contra M. gallisepticum y con un anticuerpo monoclonal contra histidina. El polipéptido se purificó mediante cromatografía de afinidad con quelatos metálicos...
Tipo: Journal article
Palavras-chave: Mycoplasma gallisepticum; Clonaje; Expresión; Purificación; PCR; Cromatografía de afinidad por quelatos metálicos níquel-ácido nitrilotriacético (Ni-NTA).
Ano: 2011
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2011000100005

DETECCIÓN DE PESTIVIRUS POR REVERSO TRANSCRIPCIÓN ACOPLADA A REACCIÓN EN CADENA DE LA POLIMERASA DE LA REGIÓN 5' cDNA NO TRADUCIDA
Provedor de dados: Revista de Salud Animal
Autores: Díaz de Arce,Heidy; Pérez,L.J.
El género Pestivirus de la familia Flaviviridae incluye diferentes especies virales que causan pérdidas importantes a la industria ganadera en todo el mundo afectando bovino, ovino/caprinos y cerdos. La detección rápida, sensible y específica de estos agentes es por lo tanto esencial para el diagnóstico y control de estas infecciones virales. Se desarrolló y estandarizó un ensayo de RT-PCR para la detección específica de pestivirus en suero basado en una pareja de cebadores que amplifica un fragmento de 119 pb de una región altamente conservada en el extremo 5´UTR del genoma ARN del virus. Este ensayo mostró ser propósito específico, sensible y altamente repetible.
Tipo: Journal article
Palavras-chave: Diagnóstico; Pestivirus; PCR.
Ano: 2008
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2008000300002

DETECCCIÓN DE Anaplasma marginale EN BOVINOS, MEDIANTE LA AMPLIFICACIÓN POR PCR DEL GEN msp5
Provedor de dados: Revista de Salud Animal
Autores: Corona,Belkis; Martínez,Siomara.
Anaplasma marginale es una rickettsia del genogrupo II de las Ehrlichias, que parasita los eritrocitos maduros del ganado bovino. Hasta el momento no se cuenta con un método de control eficaz contra la enfermedad, por lo que resulta de gran importancia desarrollar un inmunógeno capaz de prevenir la infección con este patógeno. A esto se le agrega la necesidad de contar con técnicas de diagnóstico más sensibles para ser utilizadas en el movimiento internacional de ganado hacia zonas libres de la enfermedad, que permitan la detección de animales portadores, así como para conocer la prevalencia de la enfermedad en las regiones tropicales y subtropicales. El gen msp5 está representado en el genoma como una simple copia, altamente conservado entre todas las...
Tipo: Journal article
Palavras-chave: Anaplasma marginale; Diagnóstico; PCR; Gen msp5.
Ano: 2011
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2011000100004

Antimicrobial Resistance and genes associated to the host-microbe interaction of Pasteurella multocida isolates from swine in Western Cuba
Provedor de dados: Revista de Salud Animal
Autores: Espinosa,Ivette; Báez,M; Vichi,J; Martínez,Siomara.
Pasteurella multocida is an important veterinary pathogen that causes a range of animal diseases, including fowl cholera, hemorrhagic septicemia in cattle and atrophic rhinitis in swine. P. multocida is generally recognized as a secondary invader contributing substantially to respiratory diseases in pigs by aggravation of lung lesions. Five capsule serogroups are routinely identified in P. multocida (A, B, D, E, and F) and each is generally associated with, but not completely restricted, to a specific host. A total of 16 isolates of Pasteurella multocida capsular genotype A and two isolates capsular genotype D were characterized for their susceptibilities to 10 antibiotics and the presence of four genes for virulence factors associated to adherence. The...
Tipo: Journal article
Palavras-chave: P. multocida; Virulence factor; PCR; Antimicrobial susceptibility.
Ano: 2012
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2012000300003

Tendencias en el diagnóstico de la anaplasmosis bovina
Provedor de dados: Revista de Salud Animal
Autores: González,Belkis Corona; Obregón,Dasiel; Alemán,Yousmel; Alfonso,Pastor; Vega,Ernesto; Díaz,Adrian; Martínez,Siomara.
Anaplasma marginale es una Rickettsia que parasita los eritrocitos maduros del ganado bovino, provocando grandes pérdidas económicas en las regiones tropicales y subtropicales. La enfermedad se caracteriza por marcada anemia hemolítica, disminución del peso, aborto y en ocasiones la muerte. Los animales que logran recuperarse de la fase aguda de la enfermedad permanecen persistentemente infectados y se convierten en reservorio para la transmisión de la misma a los animales sanos, por lo que se hace necesario poder contar con técnicas de diagnóstico más sensibles para ser utilizadas en el movimiento internacional de ganado hacia zonas libres de la enfermedad, que permitan la detección de animales portadores, así como para conocer la prevalencia de la...
Tipo: Journal article
Palavras-chave: Anaplasma marginale; Diagnóstico; ELISA; PCR.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000200001

Metodología diagnóstica para hemoparásitos dentro de la ganadería bovina con énfasis en la reacción en cadena de la polimerasa y su variante múltiple
Provedor de dados: Revista de Salud Animal
Autores: Bolívar,Ana María.
En la detección de algunos agentes hemáticos que comprometen la salud del ganado bovino, se emplean con mayor frecuencia, métodos parasitológicos directos y serológicos, muchas veces cuestionados dadas sus bajas especificidad y sensibilidad, adicional del tiempo consumido en la realización de los mismos. Por estas razones se discute en la presente reseña, las alternativas diagnósticas PCR y su variante múltiple como sistemas de detección alternativos con alto grado de confiabilidad y ahorros adicionales de tiempo y recursos en el entendimiento de la dinámica de infecciones hemotrópicas.
Tipo: Journal article
Palavras-chave: Diagnóstico; Trypanosoma vivax; Anaplasma marginale; Babesia bigemina; Babesia bovis; PCR; PCR multiple.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000100001

Detección de agentes hemotrópicos en una explotación ganadera utilizando PCR y DGGE
Provedor de dados: Revista de Salud Animal
Autores: Bolívar,Ana María; Rojas,Agustina; Rosales,Datty; Torres,Yzoleth; García-Lugo,Pablo.
La siguiente investigación presenta los resultados de las evaluaciones diagnósticas, realizadas por PCR, para agentes hemotrópicos, en ganado bufalino y bovino (n=77), coexistentes en una misma explotación agropecuaria. Se obtuvo una positividad de 74,03% de infección hemoparásita para el total de la población estudiada, con predominio de Anaplasma marginale sobre Trypanosoma theileri, Trypanosoma vivax y Babesia bigemina, respectivamente. Adicionalmente, algunos de los ADN problema fueron ensayados por DGGE, revelando la presencia de A. marginale y Mycoplasma wenyonii. Los resultados obtenidos corroboran la clínica presuntiva y el valor del método molecular.
Tipo: Journal article
Palavras-chave: Ganado vacuno; Ganado bufalino; Hemotrópicos; PCR; DGGE.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000100009

Detección de Mycobacterium avium paratuberculosis en caprinos ubicados en una zona semi-árida en el municipio de Tecozautla Hidalgo
Provedor de dados: Revista de Salud Animal
Autores: Méndez Olvera,Estela Teresita; Ramírez Lorenzo,Ingrid Nerina; Rojas Serranía,Nora; Olivares Orozco,Javier Lorenzo; Martínez Gómez,Daniel.
La paratuberculosis o «Enfermedad de Johne» es una enfermedad infecciosa que afecta a los rumiantes domésticos principalmente ovinos, bovinos y caprinos. El agente etiológico de dicha enfermedad es Mycobacterium avium paratuberculosis (Map) y los principales signos de esta enfermedad son pérdida de peso y diarrea crónica, ocasionados como consecuencia de la enteritis granulomatosa producida por Map. Esta enfermedad ocasiona grandes pérdidas económicas en el sector pecuario. En Estados Unidos se estimó para el 2001, una pérdida anual de 150,000 millones de dólares; en México no hay estudios económicos de las pérdidas ocasionadas por esta enfermedad y son pocos los trabajos sobre la presencia del agente etiológico en distintas áreas. El objetivo de este...
Tipo: Journal article
Palavras-chave: Mycobacterium avium paratuberculosis; Cabras; Diagnóstico; PCR.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000300006

IDENTIFICACIÓN DEL GEN TOX A EN AISLAMIENTOS CUBANOS DE Pasteurella multocida PROCEDENTES DE CERDOS MEDIANTE LA REACCIÓN EN CADENA DE LA POLIMERASA (PCR)
Provedor de dados: Revista de Salud Animal
Autores: Espinosa,Ivette; Alfonso,P; Martínez,Siomara.
Pasteurella multocida es parte de la flora comensal del tracto respiratorio superior de cerdos. Usualmente, las cepas de P. multocida del serotipo A se asocian a neumonía en animales de crecimiento y las del serotipo D codifican para una toxina de 145 kDa asociada a la rinitis progresiva. En este trabajo se realizó la caracterización bioquímica y molecular de 30 cepas de P.multocida aisladas a partir de cerdos con clínica respiratoria. Todos los aislamientos presuntivos de P.multocida fueron positivos para los cebadores KMT1SP6 y KMT1T7, amplificando un producto de 460 pb. La presencia del gen tox A solo se detectó en 4 muestras, demostrando la baja frecuencia de cepas toxigénicas y mayor prevalencia de cepas de P.multocida asociadas a procesos neumónicos...
Tipo: Journal article
Palavras-chave: Pasteurella multocida; PCR; Rinitis atrófica; Cerdos.
Ano: 2009
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000300010

EVALUACIÓN DE DIFERENTES MÉTODOS DE EXTRACCIÓN DE ADN DE MICOPLASMAS PARA SU EMPLEO EN EL DIAGNÓSTICO POR PCR
Provedor de dados: Revista de Salud Animal
Autores: Hernández,Yenney; Lobo,Evelyn; Martínez,Siomara; Zamora,Loidy.
El objetivo del presente trabajo fue evaluar diferentes métodos de extracción de ADN de micoplasmas, para su empleo en el diagnóstico por PCR. La caracterización de la cepa de Mycoplasma arginini utilizada como control positivo, se realizó con el empleo de los estudios morfológicos y las pruebas bioquímicas y enzimáticas. A continuación se procedió a la obtención de un cultivo homogéneo de la cepa de Mycoplasma arginini, que permitiera evaluar tres métodos de extracción de ADN de micoplasmas para su utilización en el ensayo de PCR. Se probaron los métodos de extracción fenol _cloroformo, centrifugación y centrifugación con choque térmico en muestras clínicas de cultivos celulares, sueros y productos biofarmacéuticos, que habían sido testadas por el método...
Tipo: Journal article
Palavras-chave: Micoplasmas; Diagnóstico; Cultivos celulares; PCR.
Ano: 2009
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000200006

IDENTIFICACIÓN DEL GEN syl EN AISLAMIENTOS CUBANOS Streptococcus suis PROCEDENTES DE CERDOS MEDIANTE LA REACCIÓN EN CADENA DE LA POLIMERASA (PCR)
Provedor de dados: Revista de Salud Animal
Autores: Espinosa,Ivette; Domínguez,Patricia; Lobo,Evelyn; Alfonso,P; Martínez,Siomara.
Streptococcus suis es un coco anaerobio facultativo, considerado un importante agente asociado a meningitis, artritis, endocarditis y neumonía en cerdos. En este trabajo se realizó el aislamiento de S.suis a partir de diferentes condiciones clínicas como neumonía, abscesos en articulaciones y tonsilas de animales asintomáticos. Se identificaron 14 cepas de S. suis: 11 a partir de pulmones, 2 de abscesos y 1 de tonsila. Las cepas mostraron diferentes patrones de pruebas bioquímicas. El gen que codifica para la hemolisina denominados suilisina (syl) se detectó por PCR solamente en 5 cepas de las cuales 4 proceden de pulmón y una de tonsila. Sin embargo, en los aislamientos procedentes de abscesos y asociados a artritis no se detectó la presencia de este gen,...
Tipo: Journal article
Palavras-chave: Streptococcus suis; PCR; Suilisina; Cerdos; Streptococcu suis; PCR; Suilysine; Pigs.
Ano: 2010
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2010000100007

DISEÑO DE UN ENSAYO DE REACCIÓN EN CADENA DE LA POLIMERASA PARA LA DETECCIÓN DE Herpesvirus bovino tipo 1
Provedor de dados: Revista de Salud Animal
Autores: Rodríguez,Majela; Avila,Mislay; Díaz de Arce,Heidy; Barrera,Maritza.
Se describe el diseño de un ensayo de reacción en cadena de la polimerasa (PCR) para la detección de herpesvirus bovino tipo 1 (HVB-1). Este se basó en la amplificación de una porción del gen tk viral, para lo cual se ensayaron diferentes concentraciones de los cebadores, de cloruro de magnesio y diferentes temperaturas de alineamiento. Además, se evaluaron los parámetros de desempeño analítico, sensibilidad y especificidad, con resultados satisfactorios. Bajo las condiciones óptimas se logró la amplificación de la banda de 202 pb esperada y se demostró la utilidad del ensayo para la identificación de aislados.
Tipo: Journal article
Palavras-chave: Herpesvirus bovino tipo 1; Diagnóstico; PCR.
Ano: 2007
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2007000100003

Priorities in seed pathology research
Provedor de dados: Scientia Agricola
Autores: Nameth,S.T.
Seed pathology as a subdisipline of plant pathology is relatively new. Paul Neergard is considered the father of seed pathology. Recent developments in the area of seed pathology technology allow for more ecofriendly seed treatments and more reliable seed health testing. Due to economics and new interest in environmental issues, research into the viability of biological seed treatments is becoming more common. The use of sophisticated DNA amplification technologies allows for the detection of seedborne pathogens that might go undetected using more conventional means. These types of research will be fundamental in guaranteeing seed health quality standards and achieving phytosanitary requirements throughout the world in the new millennium.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seedborne pathogens; Pathogen detection; Seed treatments; PCR; DNA amplification.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000500017

Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping
Provedor de dados: Scientia Agricola
Autores: Clasen,Bárbara Estevão; Silveira,Andressa de Oliveira; Baldoni,Daiana Bortoluzzi; Montagner,Daiane Fiuza; Jacques,Rodrigo Josemar Seminoti; Antoniolli,Zaida Inês.
ABSTRACT: Mycorrhizae are mutualistic associations between fungi and plant roots. These symbiotic associations are abundant and occur in 75 to 80 % of plants. Ectomycorrhizal fungi are very important in ecosystems, because their mutualistic association with plants of different species helps nutrients and water absorption, as well as protection of the host plant against pathogens and abiotic stresses. Most ectomycorrhizal fungi belong to the Basidiomycota class, such as the following genera: Amanita, Hebeloma, Hysterangium, Laccaria, Lactarius, Rhizopogon, Russula, Scleroderma, Suillus, Tricholoma, among others. Morphological studies on ectomycorrhizae report important results in understanding the species biodiversity. However, the use of molecular biology...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Ectomycorrhizal fungi; Molecular identification; Morphotype.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000300246

Bacillus cereus in Brazilian Ultra High Temperature milk
Provedor de dados: Scientia Agricola
Autores: Pacheco-Sanchez,Cristiana de Paula; Massaguer,Pilar Rodriguez de.
Brazilian Ultra High Temperature (UHT) milk consumption has increased during the last decade from 187 to 4,200 million liters. In the continuous UHT process, milk is submitted for 2-4 s to 130-150ºC, in a continuous flow system with immediate refrigeration and aseptical packing in hermetic packages. This research had the purpose to verify the incidence of B. cereus species from the B. cereus group, in UHT milk. In 1998 high indexes of these organisms were reported, reaching 34.14% of the analyzed samples. Beyond this fact, there was the need to establish methods and processes adjusted for correct identification of B. cereus. Thus, commercial sterility tests of 6,500 UHT milk packages were investigated in two assays, after ten days incubation at 37ºC and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FTIR; PCR; Commercial sterility; Statistical analyses.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000200008

การสกัดดีเอ็นเอจากข้าวโพดในระดับเล็กสำหรับทำพีซีอาร์
Provedor de dados: Thai Agricultural Research Repository
Autores: Wanchai Yenpetch; Chaba Jampatong; Sanon Suksatan.
This method for instant DNA extraction from fresh corn leaf based on a simple DNA extraction with CTAB modified from Doyle and Doyle (1987) in small scale. After extraction steps, the extracted DNA was checked the quality and quantity by using a spectrophotometer to measure the absorbance at A260/A280. The result showed the feasibility of this method which was confirmed by using PCR method. The PCR product showed an expected band. Without the addition of the phenol or 2-beta-mercaptoethanol, this method could properly use in routine laboratory where DNA is used for PCR detection. In addition, this method requires less plant tissue to extract that is useful for other plant experiments.
Palavras-chave: Corn; Leaf; DNA extraction; PCR; ข้าวโพด; ใบสด; ดีเอ็นเอ; การสกัด; พีซีอาร์; คุณภาพ; สเปคโตรโฟโตมิเตอร์; อัตราการดูดกลืนแสง.
Ano: 2010
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4690

Caracterização da agressividade de isolados de Colletotrichum de maracujá amarelo com marcadores bioquímico, fisiológico e molecular
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: Almeida,Luiz Carlos C.; Coêlho,Rildo S.B.
A antracnose é a doença pós-colheita mais importante do maracujá amarelo, cujo agente etiológico, no Brasil, foi identificado como Colletotrichum gloeosporioides. Visando caracterizar o patógeno, foram obtidos 33 isolados de três regiões produtoras do estado de Pernambuco. Critérios morfológicos como cor de colônia, forma e dimensão de conídios, a produção de peritécio e o uso de primers específicos para C. acutatum, C. gloeosporioides e "Colletotrichum de Passiflora" permitiram identificar Glomerella cingulata patótipo 1, G. cingulata patótipo 2, Colletotrichum sp. de Passiflora e Colletotrichum sp. de maracujá amarelo. Inoculações em maracujá amarelo possibilitaram separar os isolados em dois grupos, um de agressividade alta (GA-1) e outro de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora edulis f. flavicarpa; Antracnose; Pós-colheita; RAPD; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400006

Distribuição do elemento transponível impala em isolados de fusarium oxysporum patogênicos e não-patogênicos ao feijoeiro
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: Zanotti,Michele G. S.; Santos,Jildete K.; Reis,Kledna C. P.; Araújo,Elza F.; Dhingra,Onkar Dev; Queiroz,Marisa V.
A variabilidade genética de 20 isolados de Fusarium oxysporum, nove não-patogênicos e 11 patogênicos ao feijoeiro (Phaseouls vulgaris), foi determinada com base na distribuição do elemento transponível impala. A presença de impala das subfamílias D e E foi determinada por experimentos de PCR, empregando oligonucleotídeos específicos para cada subfamília. Foi observada a presença de representantes das duas subfamílias na maioria dos isolados, sugerindo, portanto, que impala é um antigo componente do genoma de F. oxysporum f. sp. phaseoli. A hibridização do DNA total de cada isolado, clivado com a enzima EcoRI, com um fragmento do elemento impala da subfamíla E, mostrou uma variação nos padrões de bandas dos isolados não-patogênicos, indicando a possível...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transposon; Murcha de feijoeiro; PCR; Variabilidade genética; Patogenicidade.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000300005

Freqüência de biovares de ralstonia solanacearum em diferentes cultivares e épocas de cultivo de batata no rio grande do sul
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: MACIEL,JOÃO L.N.; DUARTE,VALMIR; SILVEIRA,JOSÉ R.P.; VAN DER SAND,SUELI T.
A presença das biovares I e/ou II de Ralstonia solanacearum em uma lavoura de batatas (Solanum tuberosum) tem influência direta no sucesso das medidas adotadas para controlar a murcha bacteriana. As biovares diferem entre si em relação à agressividade, latência e sobrevivência. Assim, um experimento de campo foi conduzido em uma área naturalmente infestada em duas épocas de cultivo com os objetivos de verificar (1) a incidência de biovares I e/ou II, (2) a relação entre biovar e época de plantio e (3) a relação entre biovar e cultivar de batata. Os isolados obtidos de plantas das cultivares Achat, Baronesa, Elvira, Macaca, Monte Bonito e Trapeira foram identificados como biovar I ou II através da PCR, utilizando os oligonucleotídeos iniciadores T3A e T5A....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Murcha bacteriana; Solanum tuberosum; PCR.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000400009

Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: Ríos,Melina Ocampo; Fernández,Paula; Carmona,Marcelo.
Leaf scald of barley caused by Rhynchosporium secalis is an important disease in Argentina. The fungus is a necrotrophic pathogen which survives in stubble, seeds and weeds. Isolation of R. secalis from seeds on artificial media usually has not been successful due to the slow growth rate of the pathogen and strong inhibition by contaminants. The objective in this work was to detect R. secalis in different genotypes of barley seeds in Argentina using the polymerase chain reaction (PCR)-based diagnostic assay. Four barley genotypes were tested in 2004: Quilmes Ayelén, Quilmes Alfa, Barke and Maltería Pampa 1004. The previously described RS8 and RS9 primers were used for the detection of R. secalis in barley seeds. A 264-bp single band was obtained for each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hordeum vulgare; Diagnosis; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000500007

Development of a scar marker for Pierce's Disease strains of Xylella fastidiosa
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: Travensolo,Regiane F.; Ciapina,Luciane P.; Lemos,Eliana G. M.
The objective of this research was to develop a primer for a polymerase chain reaction specific for Xylella fastidiosa strains that cause Pierce's Disease (PD) in grapes (Vitis vinifera). The DNA amplification of 23 different strains of X. fastidiosa, using a set of primers REP1-R (5'-IIIICGICGIATCCIGGC-3') and REP 2 (5'-ICGICTTATCIGGCCTAC-3') using the following program: 94 ºC/2 min; 35 X (94 ºC/1 min, 45 ºC/1 min and 72 ºC/1 min and 30 s) 72 ºC/5 min, produced a fragment of 630 bp that differentiated the strains that cause disease in grapes from the other strains. However, REP banding patterns could not be considered reliable for detection because the REP1-R and REP 2 primers correspond to repetitive sequences, which are found throughout the bacterial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oligonucleotide; Polymerase chain reaction; PCR; REP-PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200002

Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: FAJARDO,THOR V. M.; NISHIJIMA,MARTA; BUSO,JOSÉ A.; TORRES,ANTÔNIO C.; ÁVILA,ANTÔNIO C.; RESENDE,RENATO O.
Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: OYDV-G; LYSV; GCLV; Detection; Coat protein; PCR; Probes; Epidemiology.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300007

Estudo da interação de um begomovírus isolado de tomateiro com a mosca branca
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: Santos,Carmem D. G.; Ávila,Antonio C. de; Resende,Renato de O.
No estudo da interação de um begomovírus isolado de tomateiro (Lycopersicon esculentum) em Anápolis-GO, denominado GO-ANPL (taxonomicamente relacionado ao Tomato rugose mosaic virus, ToRMV) com o vetor Bemisia tabaci biótipo B, determinaram-se o período de acesso de aquisição do vírus (PAA), o período de acesso de inoculação (PAI), o período de latência (PL), a sua retenção e transmissão à progênie. Nos experimentos empregaram-se plantas de tomateiro 'Santa Clara' e cinco insetos por planta. Constatou-se um PAA mínimo de 15 min com o qual 6% dos tomateiros foram infetados. Este percentual aumentou para 65% quando o PAA foi estendido para 24 h. O PAI mínimo foi de 30 min registrando-se 18% de infecção, o qual foi elevado para 67% com 24 h de PAI....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interação vírus-vetor; Geminiviridae; Lycopersicon esculentum; PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000600013

Análisis filogenético del murciélago hematófago Desmodus rotundus en el Valle del Cauca Colombia
Provedor de dados: Acta Agronómica
Autores: Castro Castro,Fernando Favian; Muñoz Flores,Jaime Eduardo; Uieda,Wilson.
En los municipios La Victoria, Águila, Obando, Cartago, Zarzal, San Pedro, Cerrito y Palmira del departamento Valle del Cauca; Mercaderes en el Cauca; y Puerto Nariño en el departamento del Amazonas, se realizó el análisis de filogenia del murciélago hematófago Desmodus rotundus. Las muestras de tejido epitelial fueron tomadas en el patagio del quiróptero, amplificadas por PCR utilizando cebadores 16SL y 16SH, se amplificó ADN mitocondrial, y se secuenciaron 50 individuos de murciélagos hematófagos. Después de la secuenciación se encontraron ocho haplotipos y se encontró que el más frecuente se presenta en 43 individuos. Se utilizaron dos métodos, máxima verisimilitud y parsimonia. Utilizando el Genbank se revisaron secuencias de marcadores mitocondriales...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ADN; Clados; Genbank; Haplotipos; Patagio; PCR; 16SL; 16SH.
Ano: 2016
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122016000100010

Quality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of development
Provedor de dados: Acta Agronómica
Autores: Henao Ramírez,Ana María; Salazar Duque,Héctor Jaime; Urrea Trujillo,Aura Inés.
Abstract Theobroma cacao L. and its products are consumed worldwide. Those products are of great research interest due to antioxidant properties of some of their polyphenolic constituents. The amount of these polyphenols and polysaccharides has shown that can interfere with the high quality and quantity of nucleic acids for molecular research. Therefore, cocoa DNA extraction protocols can require a large amount of plant material and optimization time according with plant material source. The aim of this study was to evaluate the quality and quantity of DNA isolated from field plant leaves at different developmental stages from TSH565 genotype using different DNA extraction protocols. In addition, DNA extraction protocol was evaluated for small amount of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA isolation; Field cocoa plants; In vitro cocoa plantlets; Leaf development; PCR; SSRs.
Ano: 2018
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000200311

Occurrence and dístríbutíon of Bemisia tabaci and Trialeurodes vaporariorum (Hemiptera: Aleyrodídae) on tomato crops in Panama
Provedor de dados: Acta Agronómica
Autores: Herrera Vásquez,José Angel; Jaén Sanjur,José Natividad; Zachrisson Salamina,Bruno Alexis; Rubio Miguélez,Luis; Barba Alvarado,Anovel Amet; Aguilera Cogley,Vidal Antonio; Valdespino,Randy Atencio; Torregrosa 0,Luis Galipienso; Mejía Franco,Luis Carlos.
Abstract The main open-field and greenhouse producer regions of tomato in Panama (Los Santos, Herrera, Veraguas and Chiriquí provinces) were surveyed to determine the occurrence and distribution of Bemisia tabaci and Trialeurodes vaporariorum during the growing seasons of 2017 and 2018. Twenty-two plots were surveyed, and DNA extracts of 112 adult whiteflies were analyzed by multiplex PCR, using a set of specific primers of B. tabaci MEAM1, B. tabaci NW, and T. vaporariorum. DNA extracts corresponding to 57 individual whiteflies (50.9 %) rendered PCR products of expected size with B. tabaci-specific primers (37 belonged to B. tabaci MEAM1 and 20 to B. tabaci NW), whereas the remaining DNA extracts corresponding to 55 individual whiteflies (49.1 %) rendered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Altitude; PCR; Phylogenetic; Sequencing; Weather conditions; Whiteflies.
Ano: 2022
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122022000100096

IDENTIFICACIÓN MOLECULAR DE MICROORGANISMOS ASOCIADOS A LA RIZOSFERA DE PLANTAS DE VAINILLA EN COLOMBIA
Provedor de dados: Acta Biológica Colombiana
Autores: ÁLVAREZ,CLAUDIA L; OSORIO,NELSON W; MARÍN MONTOYA,MAURICIO.
El cultivo de vainilla es altamente promisorio en Colombia, pero se requiere mayor conocimiento de su manejo agronómico y de los microorganismos que crecen asociados a su rizosfera, de los cuales depende esta planta para su nutrición y crecimiento. En este trabajo se realizaron aislamientos de microorganismos de la rizosfera de plantas de vainilla en un cultivo piloto ubicado en el municipio de Sopetrán (Antioquia, Colombia). Los microorganismos se aislaron en medios selectivos de crecimiento para evaluar su capacidad para descomponer celulosa, proteínas, solubilizar fosfato inorgánico y orgánico (fitato) y fijar nitrógeno en forma asimbiótica. Una vez aislados y purificados, se obtuvieron un total de 109 aislamientos, de los cuales se seleccionaron 52...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ADNr; Filogenia; PCR; PGPR; Vanilla planifolia.
Ano: 2013
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2013000200007

IDENTIFICACIÓN MOLECULAR DE HONGOS AISLADOS DE TEJIDOS DE FRÍJOL CON SÍNTOMAS DE ANTRACNOSIS
Provedor de dados: Acta Biológica Colombiana
Autores: VANEGAS BERROUET,KATHERIN M.; GUTIÉRREZ SANCHEZ,PABLO A.; MARÍN MONTOYA,MAURICIO A.
En este estudio se realizó el aislamiento de hongos en tejidos foliares y vainas de fríjol con síntomas de antracnosis, procedentes de cultivos de diferentes municipios del departamento de Antioquia (Colombia). La identificación de los aislamientos se realizó con base en la secuenciación de las regiones ITS del ADN ribosomal y se confirmó por observación microscópica de estructuras reproductivas en aquellos aislamientos que esporulaban en medios de cultivo. En todas las muestras sintomáticas, se logró el aislamiento del agente causal de la antracnosis, .Colletotrichum lindemuthianum, siendo confirmada su identidad por PCR dúplex con cebadores específicos CD1/CD2 y CY1/CY2. En adición se obtuvieron 17 hongos endófitos, 14 de los cuales no esporularon en...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ADNr; Endófitos; PCR; Phaseolus vulgaris.
Ano: 2014
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2014000200002

DETECCIÓN Y DIFERENCIACIÓN DE Mycoplasma gallisepticum Y Mycoplasma synoviae MEDIANTE LA TÉCNICA DE PCR A PARTIR DE HISOPOS TRAQUEALES DE AVES CON SÍNTOMAS RESPIRATORIOS
Provedor de dados: Acta Biológica Colombiana
Autores: VENTURA,CESAR E; RAMÍREZ,GLORIA; VERA,VÍCTOR.
Los micoplasmas son importantes patógenos en las aves por ser responsables de cuadros respiratorios que ocasionan grandes pérdidas económicas a la industria avícola alrededor del mundo. Existen principalmente dos especies de micoplasmas como causantes de enfermedad en aves comerciales, el Mycoplasma gallisepticum(MG) y el Mycoplasma synoviae(MS). Teniendo en cuenta su importancia y la necesidad de conocer y diferenciar las diferentes especies de micoplasmas presentes en las explotaciones avícolas, se tomaron 91 muestras de hisopos traqueales de aves con síntomas respiratorios, provenientes de igual número de granjas de pollo de engorde, ponedoras comerciales y reproductoras pesadas ubicadas en los departamentos de Cundinamarca y Boyacá, Colombia, y se...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma gallisepticum; Mycoplasma synoviae; PCR.
Ano: 2012
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000300005

Chlamydia psittaci EN AVES PSITACIDAS EN DOS PARQUES ZOOLÓGICOS DE VENEZUELA
Provedor de dados: Acta Biológica Colombiana
Autores: RODRÍGUEZ-LEO,Carlos; HERNÁNDEZ,Vianellys; ABOU ORM,Sandra; DÍAZ,Yender; CAMACHO,Daria; ARRAIZ,Nailet; USECHE,Elianee.
RESUMEN La determinación de Chlamydia psittaci (Cp) en aves psitácidas en parques zoológicos de Venezuela representa una estrategia de conservación y preservación para este grupo de aves, donde múltiples especies se encuentran amenazadas de extinción y otras han perdido su capacidad de reincorporación a su hábitat natural. A través de la reacción en cadena de la polimerasa anidada (PCR) fue amplificada la subunidad 16S del ADNr de Cp en 50 muestras de hisopado cloacal de aves psitácidas, reportando una frecuencia de 62 %. El trabajo fue realizado en el Parque Zoológico Las Delicias (PZD) 8 % y el Aquarium de Valencia (AV) 54 %. La elevada frecuencia fue asociada a un genotipo de baja concentración y virulencia debido a la ausencia de signos clínicos de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlamydia psittaci; Psitácidas; PCR.
Ano: 2017
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2017000300394

Importância da aplicação de critérios morfológicos não-clássicos para o diagnóstico citopatológico de Papillomavirus humano (HPV) previamente detectado por PCR
Provedor de dados: Acta Bioquímica Clínica Latinoamericana
Autores: Nobuyoshi Kaneshima,Edilson; Suzuki,Linda Emiko; Taguti Irie,Mary Mayumi; Yoshida,Celina Shizue; Francieli Maia da Silva,Sônia; Lopes Consolaro,Márcia Edilaine.
Existe atualmente grande preocupação com a melhoria no diagnóstico citopatológico para HPV e para isto tem-se estudado a introdução de critérios morfológicos não-clássicos, tendo em vista a elevada freqüência desta infecção viral bem como o seu potencial carcinogênico. Desta forma, este trabalho objetivou uma releitura de 41 casos positivos para HPV por PCR, cujos critérios inicialmente utilizados foram apenas os clássicos, e introduzir os critérios não clássicos. Os critérios citopatológicos mais observados foram os não clássicos, sendo bi ou multinucleação o mais freqüente, com n=37 (90,24%), seguido de núcleo hipercromático, com n=35 (85,36%) e núcleo em borrão, com n=24 (58,54%). Quanto aos critérios clássicos, a disqueratose foi observada em n=17...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV; PCR; Critérios não clássicos.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572005000100010

Optimização da detecção do polimorfismo Ala 677 - Val do gene que codifica a metilenotetrahidrofolato redutase (MTHFR)
Provedor de dados: Acta Bioquímica Clínica Latinoamericana
Autores: Persuhn,Darlene Camati; Soares Melo,Sandra; Maccagnan,Carlos Leonardo; Besen,Pinheiro; Sansão,Fabiano; Mangrich,Iriane Eger.
A mutação (C677T) no gene que codifica a enzima metilenotetrahidrofolato redutase (MTHFR) tem sido associada a hiperhomocisteinemia e possivelmente ao risco elevado para doenças vasculares. O objetivo deste trabalho foi otimizar sua detecção por PCR para faciliar estudos populacionais. Foram testadas metodologias alternativas de purificação do DNA genômico humano e os resultados obtidos permitiram padronizar uma técnica com redução de reagentes para extração de DNA por precipitação com NaCl. Foram realizados experimentos variando a concentração dos componentes da reação de PCR que permitiram definir que reações contendo metade do volume inicial de deoxinucleotídeos e iniciadores (primers), assim como 0,5U de Taq DNA polimerase produzem essencialmente a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metilenotetrahidrofolato redutase; C677T; PCR.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572006000100015

Caracterização morfofisiológica e identificação molecular de isolados de Guignardia citricarpa, agente patogênico da mancha preta dos citros - DOI: 10.4025/actasciagron.v30i5.5962
Provedor de dados: Acta Scientiarum: Agronomy
Autores: Caixeta, Marilda Pereira; UEM; Corazza, Maria Júlia; UEM; Oliveira, Ricardo Ribeiro de; UEM; Zanutto, Carlos Alexandre; UEM; Nunes, William Mário de Carvalho; UEM; Vida, João Batista; UEM.
O presente trabalho teve como objetivo identificar 11 isolados de Guignardia citricarpa, agente causal da mancha preta dos citros (MPC), obtidos de frutas cítricas sintomáticas de diferentes regiões geográficas, por meio da PCR e caracterização morfofisiológica das estruturas propagativas, esporulação e crescimento micelial em diferentes meios de cultura, temperaturas e regimes de luz, nas condições de laboratório.  Pelo teste de PCR, todos os isolados foram identificados como o patógeno G. citricarpa. Os isolados caracterizados foram submetidos às temperaturas de 20, 25 e 30ºC, em regime de luz contínua, escuro contínuo e fotoperíodos de 12 horas, durante 24 dias. Utilizaram-se os meios de cultura aveia-ágar (AA), batata-dextrose-ágar (BDA) e...
Palavras-chave: 5.01.02.00-1 Fitossanidade G. citricarpa; PCR; Esporulação; Crescimento micelial; Temperatura; Meio de cultura; Fotoperíodo. G. citricarpa; PCR; Sporing; Mycelial growth; Temperature; Mean of culture; Photoperiod.
Ano: 2008
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5962

Genetic and taxonomic relationships of five species of Rallidae (Aves: Gruiformes) based on mitochondrial cytochrome oxidase subunit I sequences
Provedor de dados: Acta Scientiarum: Animal Sciences
Autores: Deef, Lamiaa Elsayed Mokhtar.
This is the first study to detection the genetic relationship between Porphyrio alleni and four Rallidae species: Fulica atra, Gallinulla angulata, Gallinulla chloropus and Porphyrio porphyrio. Mitochondrial cytochrome oxidase subunit I (COI) sequences were used as an effective marker in this study. DNA of Rallidae species were extracted, amplified using polymerase chain reaction (PCR) and then sequenced. The results obtained from information based on COI sequences revealed that Gallinulla angulata and Gallinulla chloropus fall into two separate clades and they are not monophyletic. This suggests that, two moorhens could not be laid into the same genus. In addition, Porphyrio porphyrio was included in the same genus with Porphyrio alleni but they were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birds; Gruiformes; PCR; Phylogeny; Sequencing; Species.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/37184

Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis - doi: 10.4025/actascibiolsci.v32i4.6276
Provedor de dados: Acta Scientiarum: Biological Sciences
Autores: Costa, Geraldo Márcio da; UFLA; Paiva, Luciano Vilela; UFLA; Piccoli, Roberta Hilsdorf; Universidade Federal de Lavras; Figueiredo, Demétrio Junqueira; Universidade Federal de Lavras; Pereira, Ulisses de Pádua; UFLA; Silva, Nivaldo da; EV-UFMG.
Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Three hundred fourty four strains of coagulase-positive Staphylococcus (CPS), isolated from mastitis cases, underwent phenotypic and genotypic tests to evaluate the efficiency of a simplified key, based on phenotypic tests for the discrimination of these microorganisms. The tests consisted of amplification of the femA gene and hemolysis in blood agar, production of acetoin and fermentation of maltose, mannitol and trehalose. Strains that showed negative results in the amplification test of the femA gene or that were not identified as Staphylococcus aureus (S. aureus) by phenotypic tests were tested with the APISTAPH kit...
Tipo: Pesquisa laboratorial
Palavras-chave: 5.05.02.03-4 mastitis pathogens; Characterization; Coagulase test; PCR; Biochemical tests Doenças Infecciosas dos Animais mastitis pathogens; Characterization; Coagulase test; PCR; Biochemical tests.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6276

Identificação do gene mcyA em florações naturais de Radiocystis fernandoi, em um tributário do reservatório de Rosana, Brasil - doi: 10.4025/actascibiolsci.v33i3.6802
Provedor de dados: Acta Scientiarum: Biological Sciences
Autores: Fonseca, Iraúza Arroteia; UEM; Maniglia, Thiago Cintra; UEM; Rodrigues, Liliana; UEM; Prioli, Alberto José; UEM; Prioli, Sônia Maria Alves Pinto; UEM.
As cianobactérias são conhecidamente produtoras de toxinas. Dentro de uma mesma espécie, podemos encontrar variedades tóxicas e não-tóxicas, impossíveis de diferenciação apenas pela morfologia. A principal toxina produzida pelas cianobactérias é a microcistina. Esta proteína é biossintetizada por um grupo de genes conhecidos como mcy. A detecção destes genes a partir de PCR permite a distinção das variedades tóxicas e não-tóxicas. Desse modo, o objetivo desse trabalho foi investigar a ocorrência de florações produtoras de toxinas em um rio tributário do reservatório de Rosana, via amplificação do gene mcyA por PCR. Foram coletadas duas amostras de água da subsuperfície. As duas amostras coletadas no rio do Corvo foram dominadas pela espécie Radiocystis...
Tipo: Toxicidade
Palavras-chave: Ecologia Cianobactéria; Toxina; PCR; Radiocystis fernandoi; McyA Genética Cyanobacteria; Toxins; PCR; Radiocystis fernandoi; McyA.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6802

Dados clínicos, laboratoriais e epidemiológicos de pacientes chagásicos residentes no noroeste do Paraná, Brazil
Provedor de dados: Acta Scientiarum: Biological Sciences
Autores: Gomes, Mônica Lúcia; UEM; Cassarotti, Dirceu José; 15ª Regional do Paraná; Ramos, Miria; UEM; Zanzarini, Paulo Donizete; UEM; Galvão, Lúcia Maria Cunha; UFMG; Chiari, Egler; UFMG; Marques-Araújo, Silvana; UEM.
Neste trabalho, foram avaliados os dados clínicos, laboratoriais e epidemiológicos de 48 pacientes chagásicos residentes na região noroeste do Paraná. No estudo clínico, o eletrocardiograma e o raio-X de tórax estavam alterados em 59,5% e 27% dos pacientes, respectivamente. Dos testes laboratoriais realizados antes de se propor o tratamento etiológico, a IFI e ELISA apresentaram resultados positivos em todos os pacientes, exceto um caso com título da IFI 20. A hemocultura, LMCo e PCR foram positivas em 41,7%, 89,6% e 83,3%, respectivamente. Observamos que 52,1% da população era feminina e 47,1% masculina com idade média de 46,9 &amp;plusmn; 10,1 anos. Quanto à procedência, 70,9% eram procedentes de Minas Gerais e São Paulo e 18,8% relataram ser...
Palavras-chave: 2.00.00.00-6 Ciências Biológicas doença de Chagas; Hemocultura; PCR; Anticorpos líticos; Dados epidemiológicos; Noroeste do Paraná 2.00.00.00-6 Ciências Biológicas.
Ano: 2000
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2967

DETECCIÓN DE UN DEFECTO GENÉTICO EN BOVINOS MEDIANTE UNA PRUEBA DE ADN.
Provedor de dados: Agricultura Técnica
Autores: Felmer D.,Ricardo; Butendieck B.,Norberto; Butendieck B.,Bárbara; Villegas M.,Juana.
La deficiencia en la capacidad de unión de leucocitos bovinos a los antígenos, más conocida como BLAD, es una enfermedad hereditaria que resulta letal para el ganado de la raza Holstein. Su principal característica es ser una enfermedad autosómica recesiva que puede ser transmitida a la descendencia. El objetivo del trabajo fue estandarizar la metodología para realizar un diagnóstico de la enfermedad mediante técnicas moleculares y lograr una primera aproximación sobre la frecuencia génica del alelo mutado en algunas poblaciones de toros de la IX y X Región. En base a la amplificación del ADN mediante reacción en cadena de polimerasa (PCR) y posterior digestión con enzimas de restricción Taq I y Hae III, fue posible visualizar, mediante electroforesis en...
Tipo: Journal article
Palavras-chave: Deficiencia de adhesión leucocitaria bovina; BLAD; PCR; RFLP; Holstein; Enfermedad.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072001000100005

Evaluación de Cepas Nativas de Bacillus thuringiensis Como una Alternativa de Manejo Integrado de la Polilla del Tomate (Tuta absoluta Meyrick; Lepidoptera: Gelechiidae) en Chile
Provedor de dados: Agricultura Técnica
Autores: Niedmann Lolas,Lorena; Meza-Basso,Luis.
La polilla del tomate (Tuta absoluta Meyrick; Lepidoptera: Gelechiidae) es la plaga más devastadora del cultivo del tomate (Lycopersicon esculentum Mill.) en Chile, produciendo pérdidas del 60 al 100% en campos no tratados con insecticidas. Puesto que las plagas de insectos están desarrollando niveles de resistencia a los insecticidas convencionales, existe interés por estrategias de control que incluyen el empleo de biopesticidas. En el presente trabajo se estudió el potencial de aislados nativos de Bacillus thuringiensis (Bt) con actividad tóxica contra esta plaga. Los aislados de Bt fueron colectados de muestras de suelo provenientes de la VII Región del país, y fueron caracterizados empleando diferentes criterios: morfología de la colonia y de la...
Tipo: Journal article
Palavras-chave: Bioensayos; PCR.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072006000300002

Selección de Genotipos Chilenos de Trigo Portadores del Alelo Glutenina HMW Glu-D1 x5 Mediante Reacción en Cadena de la ADN Polimerasa
Provedor de dados: Agricultura Técnica
Autores: Zúñiga,Javier; Rathgeb,Paola; Jobet,Claudio; Campos,Hugo.
La calidad panadera es un importante elemento para el desarrollo de nuevas variedades de trigo (Triticum aestivum L.) y se asocia principalmente con un conjunto de proteínas conocidas como gluteninas de elevado peso molecular (HMW), aún cuando existen otras proteínas relacionadas con la calidad panadera. Los procesos actuales de selección de genotipos con calidad panadera se basan en el contenido de gluten de la masa o la identificación de alelos codificadores de gluteninas HMW mediante electroforesis. Se reporta un método basado en la identificación, mediante la reacción en cadena de la ADN polimerasa (PCR), del alelo codificador de la glutenina HMWx5, la cual se asocia con calidad panadera. Mediante el uso de partidores apropiados se amplificó de forma...
Tipo: Journal article
Palavras-chave: Mejoramiento genético; Trigo; Triticum aestivum L.; Biotecnología; PCR.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072004000300001

Actividad in vitro del extracto acuoso del Bonellia flammea contra hongos fitopatógenos
Provedor de dados: Agrociencia
Autores: Moo-Koh,F. Amalia; Cristóbal Alejo,J.; Reyes-Ramírez,Arturo; Tun-Suárez,J. María; Sandoval-Luna,Ruth; Ramírez-Pool,J. Abrahán.
Las enfermedades causadas por hongos en las plantas disminuyen la calidad y el rendimiento de los cultivos; el control de estas enfermedades demanda investigación enfocada a reducir el uso de los fungicidas sintéticos, los cuales generan resistencia de cepas y contaminación al medio ambiente. El extracto acuoso de Bonellia flammea es una alternativa natural de control ya que actúa como fungicida. El objetivo del presente estudio fue evaluar in vitro el efecto del extracto acuoso de B. flammea contra siete hongos fitopatógenos. El diseño experimental fue completamente al azar, los tratamientos fueron cada hongo expuesto al extracto y el hongo sin extracto, la unidad experimental fueron cuatro cajas petri, con cuatro repeticiones, los datos se analizaron...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inhibición; Control; Fungosis; PCR.
Ano: 2014
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952014000800006

Infection by the microsporidium of Clado Nosema/Vairimorpha in pupal parasitoids
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: PAES,JOÃO P.P.; CARVALHO,VANESSA R.; SOUZA,AMANDA R. DE; WILCKEN,CARLOS F.; BUENO,REGIANE C.O. F.
Abstract: The sugarcane borer, Diatraea saccharalis is one of the hosts more used for parasitoid pupal multiplication in Brazil. The parasitoids pupal of Trichospilus diatraeae and Palmistichus elaeisis are generalist natural enemies with potential to suppress populations of diverse families of lepidopteran pests. The success in the utilization of these natural enemies in the field is directly related to the capacity of search of the host, this capacity might be affected by the presence of the pathogens. In this context, the aim of this essay was to detect the presence of intracellular parasites of Phylum Microsporidia. These pathogens may cause morphological and behavioral alterations. The presence of infection was verified by microscopy and was confirmed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Microsporidium; Pupal parasitoids; PCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500857

Real-time PCR for detection of Brucella ovis and Histophilus somni in ovine urine and semen
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Moustacas,V.S.; Silva,T.M.A.; Costa,É.A.; Costa,L.F.; Paixão,T.A.; Santos,R.L.
RESUMO A epididimite infecciosa ovina é uma das principais enfermidades reprodutivas de carneiros. O presente estudo teve por objetivo desenvolver protocolos de PCR em tempo real para B. ovis e H. somni e avaliar sua aplicabilidade em amostras de sêmen e urina de carneiros. Delinearam-se primers e sondas espécie-específicos para cada agente. As sondas foram delineadas com o sistema TaqMan incorporando um marcador FAM para B. ovis e Cy5 para H. somni na extremidade 5' e um quencher na extremidade 3'. A PCR em tempo real para B. ovis e H. somni foi altamente sensível, uma vez que a amplificação de DNA ocorreu com até 0,2ng de DNA/reação. A especificidade dos iniciadores e sondas foi avaliada com amostras de DNA de outros agentes causadores de epididimite...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious epididymitis; Ovine; Diagnosis; PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601751

Detecção dos genes da toxina citoletal distensiva em estirpes de Campylobacter jejuni isoladas de carcaças de frangos
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Carvalho,A.F.; Silva,D.M.; Azevedo,S.S.; Piatti,R.M.; Genovez,M.E.; Scarcelli,E.
Foram analisadas 80 amostras de sobrecoxas de frangos de corte resfriados provenientes de feiras livres e hipermercados do município de São Paulo, SP. Treze estirpes de Campylobacter spp. foram isoladas em 10 (12,5%) sobrecoxas, sendo cinco amostras originárias de feiras livres e cinco de hipermercados. Onze estirpes foram identificadas como Campylobacter jejuni e duas como Campylobacter coli. As 11 estirpes foram confirmadas como C. jejuni pela PCR do gene da hipuricase (hip), e destas, quatro (36,4%) apresentaram os três genes (cdtA, cdtB e cdtC) codificantes da toxina citoletal distensiva pela multiplex-PCR, sendo três estirpes provenientes de hipermercados e uma de feira livre. Observou-se a presença de estirpes virulentas de C. jejuni, portadoras do...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Frango de corte; Campylobacter jejuni; Toxina citoletal distensiva; PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000500006

Detecção de Listeria monocytogenes pela técnica de PCR em leite contaminado artificialmente
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Peres,N.D.; Lange,C.C.; Brito,M.A.V.P.; Brito,J.R.F.; Arcuri,E.F.; Cerqueira,M.M.O.P.
Avaliou-se a técnica de PCR como opção para reduzir o tempo de detecção de Listeria monocytogenes no leite. Para tanto, amostras de leite desnatado esterilizado e de leite cru integral - com baixa, média e alta contagem de microrganismos aeróbios mesófilos - foram inoculadas experimentalmente com diversas concentrações de L. monocytogenes. Os resultados da reação de PCR foram comparados com os da cultura da amostra empregando-se metodologia padronizada tradicional. Não se detectou L. monocytogenes pela reação de PCR quando esta foi realizada a partir do caldo de enriquecimento de Listeria (LEB) após 24 horas de incubação, nem no leite desnatado esterilizado, nem no leite cru integral. Após 48 horas de enriquecimento em LEB, a bactéria foi detectada por PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Patógeno alimentar; Método de detecção; PCR; Segurança do leite.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400029

Detection of papillomavirus DNA in formalin-fixed paraffin-embedded equine aural plaque samples
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Zakia,L.S.; Basso,R.M.; Olivo,G.; Herman,M.; Araujo Jr.,J.P.; Borges,A.S.; Oliveira-Filho,J.P.
A placa aural é uma dermatopatia associada à quatro Equus caballus papillomavirus (EcPVs). Até o momento, o DNA de EcPVs não foi identificado em amostras de placa aural fixadas em formalina e embebidas em parafina (FFPE). O objetivo deste estudo foi otimizar um método para a detecção dos quatro tipos de EcPVs em 21 amostras FFPE usando a PCR. O DNA dos EcPVs foram detectados em 11 amostras (52.4%). O DNA do EcPV4 foi detectado em 38.1% (8/21) e do EcPV3 em 4.8% (1/21) das amostras. Coinfecção foi identificada em duas amostras (9.5%); EcPV4 e 5 foram detectados simultaneamente em uma amostra, enquanto o DNA dos EcPV4 e 6 foi detectado em outra. A especificidade do DNA dos papilomavírus equinos foi avaliada por sequenciamento gênico direto, que confirmou a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Horses; Formalin-fixed and paraffin-embedded samples; Papillomaviruses; PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000401193

Detection of Toxoplasma gondii by PCR and mouse bioassay in commercial cuts of pork from experimentally infected pigs
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Tsutsui,V.S.; Freire,R.L.; Garcia,J.L.; Gennari,S.M.; Vieira,D.P.; Marana,E.R.M.; Prudêncio,L.B.; Navarro,I.T.
The distribution of T. gondii in commercial cuts of pork (ham, tenderloin, spareribs and arm picnic) by PCR and bioassay from experimentally infected pigs, was evaluated. Eighteen mixed breed pigs were divided into two groups (G). The G1 animals (n=10) were infected with 4 x10(4) oocysts of the T. gondii VEG strain and the G2 animals (n=8) were used as control. Pigs of both groups were slaughtered at 59th day after infection, and meat samples were collected for bioassay and PCR. All animals from G1 were positive by at least one or both tests, and all control animals were negative. T. gondii was identified in pork by mouse bioassay and PCR in 27/40 (67.5%) and in 9/40 (22.5%) of the evaluated samples, respectively. There were no statistical differences in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pork meat; Mouse bioassay; PCR; Toxoplasma gondii.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000100006

Isolamento de Campylobacter jejuni em feto ovino abortado: relato de caso
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Vargas,A.C.; Cecim,M.; Viana,L.R.; Spricigo,D.A.; Costa,M.M.
Relata-se o isolamento de Campylobacter jejuni de um feto ovino abortado no terço final da gestação. Em uma semana, observaram-se quatro casos de aborto em ovelhas com quatro meses de gestação. Imediatamente após o terceiro aborto, um dos fetos foi submetido a exame patológico, quando se realizou a colheita do conteúdo do abomaso para análise bacteriológica. À necropsia, somente foram evidenciadas alterações autolíticas. Apenas na microaerofilia encontrou-se crescimento bacteriano puro com características morfo-tintoriais de Campylobacter sp., identificado fenotípica e molecularmente como C. jejuni. Alerta-se para a possibilidade de quadros de abortos em ovinos serem causados por esse patógeno.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter; Jejuni; Ovino; Aborto; PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000300007

Cytological and molecular detection of Leishmania spp. in different biological tissues of dogs in areas endemic for visceral leishmaniasis
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Dias,A.F.L.R.; Almeida,A.B.P.F.; Rodrigues,J.Y.; Nakazato,L.; Fujimori,M.; Sousa,V.R.F.
RESUMO Devido à ampla distribuição da leishmaniose visceral (LV) no Brasil e à importância dos cães no ciclo de transmissão dessa zoonose, o presente estudo teve como objetivo avaliar a ocorrência de Leishmania spp. e caracterizar a espécie circulante em diferentes tecidos biológicos de cães da Baixada Cuiabana, Mato Grosso, Brasil. Amostras de sangue, linfonodo e medula óssea foram coletadas de 205 cães para realização de análise parasitológica por citologia e análise molecular por meio da nested PCR (nPCR) e do sequenciamento . Dos 205 cães estudados, 34 (16,58%) animais foram positivos pela nPCR, dos quais 12 possuíam formas amastigotas de Leishmania spp. na citologia. Amostras positivas na nPCR foram sequenciadas e caracterizadas como Leishmania...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Visceral canine leishmaniosis; Leishmania infantum; PCR; Parasitology; Kala-azar.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000602103

Molecular detection of bovine immunodeficiency virus (BIV) in bovines from the state of Minas Gerais, Brazil
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Rodrigues,A.P.S.; Fonseca Júnior,A.A.; Lima,G.K.; Bicalho,J.M.; Leite,R.C.; Reis,J.K.P.
RESUMO O vírus da imunodeficiência bovina é o agente causador da imunodeficiência viral bovina que é conhecido por infectar bovinos em todo o mundo. Como em outras infecções por retrovírus, os hospedeiros desenvolvem uma infecção de longo prazo e a maioria dos animais infectados permanece assintomática. O objetivo deste estudo foi detectar DNA proviral BIV em amostras de sangue de bovinos e estimar a ocorrência de infecção no estado de Minas Gerais, Brasil. Amostras de sangue de 391 bovinos foram coletadas de duas regiões do estado, Zona da Mata e Central. O DNA proviral foi detectado por reação em cadeia da polimerase semi-nested (SN-PCR). Os resultados de SN-PCR indicaram uma ocorrência de BIV de 12,5% no estado. Os produtos amplificados foram...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bovine immunodeficiency vírus; PCR; Minas Gerais; Brazil.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200711

Alterações histológicas da pars esophagea de suínos e sua relação com Helicobacter spp.
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Yamasaki,L.; Boselli-Grotti,C.C.; Alfieri,A.A.; Silva,E.O.; Oliveira,R.L.; Camargo,P.L.; Bracarense,A.P.F.R.L.
A relação entre Helicobacter spp. e a presença de alterações histológicas na pars esophagea de suínos foi avaliada em 67 estômagos de animais em idade de abate. Para a identificação das helicobactérias, utilizou-se a técnica da PCR com primers específicos para o gênero Helicobacter. As alterações histológicas foram identificadas e classificadas como ulceração, erosão, degeneração epitelial, alongamento de papilas, hiperplasia, paraqueratose, intensidade do infiltrado inflamatório e aumento do número de folículos linfoides. As alterações mais frequentemente encontradas na pars esophagea foram a degeneração epitelial e o alongamento de papilas, observadas em 83,5% (n=56) das amostras analisadas. Em 77,5% (n=52) das amostras, observou-se paraqueratose e em...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suíno; Estômago; Úlcera gástrica; Helicobacter spp.; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000300005

Employment of the 18s rRNA screening PCR technique in the detection of Equine Piroplasmosis, in horses of sports and military operations, of the Brazilian Army
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Sousa,C.P.; Soares,J.G.B.
ABSTRACT The present work had the objective of detecting the occurrence of Equine Piroplasmosis in horses housed in the 3rd Guards Cavalry Regiment (GCR) - Brazilian Army (BA) ˗ Porto Alegre, RS-Brazil, as well as to demonstrate the proactivity of PCR (Polymerase Chain Reaction) technique, aiming at the judicious use of the resources involved in the training and employment of Equines in the Brazilian Army. Fifty horses of the 3rd GCR - Porto Alegre ˗ RS, which are employed for Sport, Military Ceremonial, Law and Order Guarantee Operations (LOGO), were evaluated by means of the 18s r RNA screening with PCR technique, thirty eight horses with Babesia Caballi and Theileria Equi were detected, which corresponds to an incidence of 76% of the horses effective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horses; Babesia Caballi; Detection; PCR; LOGO; GCR; BA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601680

Vírus da artrite encefalite caprina: isolamento e caracterização de parte do gene gag
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Lima,P.P.; Rocha,M.A.; Stancek,D.; Gouveia,A.M.G.; Oliveira,G.D.R.
Amostras de sangue de 12 animais soropositivos pelo teste de imunodifusão em gel de agarose e que não apresentavam sinais clínicos sugestivos de infecção pelo vírus da artrite-encefalite caprina (CAEV) foram coletadas para isolamento viral. Mácrofagos derivados de monócitos foram co-cultivados com células de membrana sinovial caprina (MSC), resultando em cinco amostras que apresentaram efeito citopático característico do tipo persistente, semelhante ao observado para o CAEV. Uma técnica de reação em cadeia de polimerase (PCR) foi padronizada para amplificar parte do gene gag do genoma pró-viral, codificante para a proteína do capsídeo viral (p25). As cinco amostras foram amplificadas pela PCR e três delas, BR-UFMG/PL1, BR-UFMG/PL2 e BR-UFMG/PL3, foram...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Vírus da artrite-encefalite caprina; Isolamento; PCR; Análise filogenética.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000200001

Aplicação da técnica de PCR na detecção de Yersinia enterocolitica em suínos abatidos sem inspeção
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Teodoro,V.A.M.; Pinto,P.S.A.; Vanetti,M.C.D.; Bevilacqua,P.D.; Moraes,M.P.; Pinto,M.S.
Avaliou-se a contaminação de carcaças e tonsilas de suínos por Y. enterocolitica em estabelecimentos de abate não inspecionados, comparando a pesquisa microbiológica convencional com a técnica da reação em cadeia de polimerase (PCR) e o tipo de amostra analisada (de tonsila ou de carcaça), como subsídio ao monitoramento microbiano em sistemas de análise de perigos e pontos críticos de controle. Calcularam-se os custos dos dois testes. Não se detectou Y. enterocolitica pela técnica microbiológica convencional, mas, pela técnica de PCR foi possível detectar a bactéria em 40% das carcaças e em 43% das tonsilas, incluindo cepas patogênicas nas tonsilas. Não houve diferença entre os resultados positivos para as amostras de tonsilas e esfregaços de superfície...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suíno; Contaminação; Yersinia enterocolitica; PCR; APPCC.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000100002

Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Resende,M.V.; Lucio,A.C.; Perini,A.P.; Oliveira,L.Z.; Almeida,A.O.; Alves,B.C.A.; Moreira-Filho,C.A.; Santos,I.W.; Hossepian de Lima,V.F.M.
The objective of the present study was to determine the sperm enrichment with X-bearing spermatozoa, after one centrifugation in a Percoll or OptiPrep continuous density gradient, using quantitative real-time polymerase chain reaction (qPCR) of sperm DNA and resultant in vitro-produced bovine embryos by PCR. Frozen/thawed sperm was layered on density gradients and the tubes were centrifuged. Supernatants were gently aspirated and the sperm recovered from the bottom of the tubes. Cleavage and blastocyst rates were determined through in vitro production of embryos and PCR was performed to identify the embryos' genetic sex. A difference in blastocyst rate was found in the Percoll treatment compared to OptiPrep (P&lt;0.05). The percentage of female embryos in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Sperm sexing; Centrifugation; Embryo sexing; QPCR; PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300002

Winter dysentery in cows associated with Bovine Coronavirus (BCoV)
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Brandão,P.E.; Gregori,F.; Sforsin,A.J.; Villarreal,L.Y.B.; Jerez,J.A.
Descreve-se a pesquisa de BCoV e rotavírus em 13 mostras fecais de vacas de surtos de disenteria utilizando uma nested PCR dirigida ao gene RdRp e PAGE, respectivamente. Todas as amostras fecais foram positivas para BCoV e nenhuma delas apresentou-se positiva para rotavírus em PAGE. O encontro de coronavírus bovino em amostras fecais de vacas com disenteria sugere que este vírus possa ser o agente primário envolvido na etiologia dos casos aqui relatados.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bovine; Winter dysentery; Coronavirus; PCR; Diagnosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000400038

Detecção de ácidos nucléicos de Brucella spp., Leptospira spp., herpesvirus bovino e vírus da diarréia viral bovina, em fetos bovinos abortados e em animais mortos no perinatal
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Cortez,A.; Castro,A.M.G.; Heinemann,M.B.; Soares,R.M.; Leite,R.C.; Scarcelli,E.; Genovez,M.E.; Alfieri,A.A.; Richtzenhain,L.J.
Samples of 114 bovine fetuses and 10 calves, which dead in perinatal period, were examined for detection of DNA. The most common detected agent was Brucella spp. in 17 samples (13.7%) followed by Leptospira spp. in 4 cases (3.2%),bovine herpesvirus (BHV) and bovine viral diarrhea (BVDV) in 3 animals (2.4%) each, and 1 for the association of BVDV and BHV. In 77.4 % (96/124) of the samples it was not possible to detect any agent.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Aborto; Feto; PCR; RT-PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600036

Detecção de Theileria equi e Babesia caballi e anticorpos anti-Ehrlichia spp. em equídeos do Pantanal Mato-Grossense, Brasil
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Barros,E.M.; Braga,Í.A.; Santos,L.G.F.; Ziliani,T.F.; Melo,A.L.T.; Borges,A.M.C.M.; Silva,L.G.; Aguiar,D.M.
O presente estudo avaliou equídeos de 19 fazendas da região do Pantanal Mato-Grossense, sendo 121 equídeos testados pela reação em cadeia pela polimerase (PCR), para detectar fragmentos dos genes dos seguintes gêneros: Babesia, Theileria, Anaplasma, Ehrlichia, e Neorickettsia, e pela reação de imunofluorescência indireta (RIFI), para detectar anticorpos anti-Ehrlichia spp. Das amostras testadas na PCR, 17 (14,0%) animais de nove (47,3%) fazendas foram positivos. Das amostras positivas, 16 foram 100% idênticas a sequencias de Theileria equi e uma foi 99% similar à sequência de Babesia caballi, todas disponíveis no GenBank. Pela RIFI, 48 (39,6%) equídeos foram soropositivos para antígenos de E. canis, sendo 40 (83,3%) amostras com títulos de 40 e oito...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cavalo; Carrapatos; PCR; RIFI; Muares.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300716

Sensibilidade da PCR na amplificação do DNA bovino em diluição seriada e mistura de amostra macho e fêmea
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Cruz,A.S.; Silva,D.C.; Costa,E.O.A.; Silva,D.M.; Abreu,D.A.; Cruz,A.D.
A mixture of bovine DNA from a male and a female Jersey (Bos taurus taurus) bred in different proportions was used to determine the sensitivity of PCR to amplify and discriminate the bovine DNA samples. Samples were obtained from the peripheral blood of a bull and a heifer and DNA was isolated using a commercial kit for extraction and purification of nucleic acids. Two primers sets were designed to flank genomic regions: one autosomal and one Y-specific. DNA samples were diluted in water to a final concentration of 4x10-14 ng. The results showed positive amplification of the samples diluted to a concentration of 4x10-10ng and 4x10-4ng for the autosomal and Y-specific regions, respectively. PCR was able to discriminate the male DNA in a mixture of 99:1 (DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; DNA bovino; Determinação sexual; Sensibilidade.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400030

Ocorrência de Cryptosporidium spp. em animais exóticos de companhia no Brasil
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Souza,M. S. de; Vieira,B. R.; Riva,H. G.; Homem,C. G.; Silva,D. C. da; Nakamura,A. A.; Meireles,M. V.
RESUMOA infecção por algumas espécies ou genótipos de Cryptosporidiumrepresenta um risco em potencial para a saúde pública, principalmente por causa de morbidade e mortalidade em crianças de zero a cinco anos de idade e em pacientes imunodeprimidos. Embora existam alguns relatos de infecção por Cryptosporidiumem animais de companhia, sua participação na epidemiologia da criptosporidiose humana é incerta, e a literatura sobre esse tema ainda é bastante escassa. O objetivo deste estudo foi determinar a ocorrência e realizar a classificação molecular deCryptosporidiumspp. em amostras fecais de animais exóticos criados como animais de estimação no Brasil. Um total de 386 amostras de seis espécies de animais foi colhido e armazenado em solução de dicromato de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Saúde pública; Zoonoses.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000501321

Análise de polimorfismos do gene da beta-lactoglobulina em vacas da raça Nelore e efeitos sobre o peso à desmama de suas progênies
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Faria,F.J.C.; Guimarães,S.E.F.; Mourão,G.B.; Lima,R.M.G.; Pinheiro,L.E.L.
Informações sobre peso à desmama de bezerros Nelore foram utilizadas após ajuste para idade padrão aos 205 dias, sexo, idade da mãe, touro e mês de desmama, para separar as reprodutrizes em dois grupos, segundo o peso de suas crias. As médias de peso dos bezerros ajustadas pelo método dos quadrados mínimos e erros-padrão (LSM± SE) foram para os grupos pesados (P) e leves (L) 163,21± 2,18kg e 134,44± 2,18kg, respectivamente, com 41 animais em cada grupo. Essas reprodutrizes foram submetidas a coleta de sangue para estudo de polimorfismos do gene da beta-lactoglobulina, por meio da técnica de PCR-RFLP. A amplificação e a digestão de um fragmento do gene da beta-lactoglobulina entre o éxon II e III identificou os genótipos 1AA, 24AB e 56BB, com as freqüências...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Nelore; Beta-lactoglobulina; PCR; RFLP; Polimorfismo genético.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000300016

Análise filogenética do gene da miogenina
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Schierholt,A.S.; Fonseca,I.; Silva,P.V.; Paiva,S.R.; Chaves,L.C.S.; Lopes,P.S.; Faria,D.A.; Guimarães,S.E.F.
Estudou-se a filogenia do gene da miogenina, um membro da família MyoD, reguladora da miogênese, que ocorre durante o desenvolvimento embrionário, e sua história evolutiva em espécies domésticas que apresentem seqüências de DNA depositadas no Genbank, comparando-se o índice de substituição de nucleotídeos não-sinônimos pelo índice de substituição sinônima. Valores maiores do que um (1) indicaram que o gene sofreu mudanças que tornaram o organismo mais adaptado ao ambiente. As árvores filogenéticas foram obtidas por máxima verossimilhança, e os índices de substituição sinônima e não-sinônima foram analisadas pelo método de parcimônia. Os resultados indicaram que, provavelmente, o gene sofreu evolução adaptativa no grupo Ruminantia, Bos taurus e Ovis aries,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Miogênese; Seqüenciamento de DNA; PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000100022

Comparison of three laboratorial tests for diagnosis of canine parvovirus infection
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Silva,M.M.O.; Castro,T.X.; Costa,E.M.; Trancoso,T.A.L.; Mendes-de-Almeida,F.; Labarthe,N.V.; Cubel Garcia,R.C.N.
The aim of this study was to evaluate the rapid tests currently used for canine parvovirus (CPV) diagnosis: hemagglutination test (HA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). A total of 112 fecal samples collected from diarrheic puppies up to one year of age were tested. The EIA was able to detect CPV antigen in 44 samples. By HA, 32 samples tested highly positive with titers &amp;gt;128, eight tested weakly positive (titers 32 and 64) and 72 were negative (titers &lt;16). Using PCR, 57 samples were found positive including 13 EIA-negative and 19 HA-negative samples. The best correlation was observed between EIA and PCR (88.4%). These tests were able to detect all types of CPV, including CPV-2c. Considering that 23%-33% of dogs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine parvovirus; Enteritis; HA; EIA; PCR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100023

Freqüência do gene Miostatina (GDF-8) em rebanhos brasileiros da raça Marchigiana
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Teixeira,C.S.; Oliveira,D.A.A.
Identificou-se e determinou-se a freqüência do gene miostatina (GDF-8) normal e mutante em rebanhos Marchigiana, em 377 bovinos da raça Marchigiana, criados nos estados de São Paulo e Paraná. Identificaram-se 37,9% de animais normais, 55,2% de portadores e 6,9% homozigotos afetados para musculatura dupla. Estes resultados indicam que os criadores têm interesse na característica musculatura dupla, promovendo, ainda que aleatoriamente, selecão a favor da mutação.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Marchigiana; DNA; Miostatina; Musculatura dupla; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300036

Avaliação da reação em cadeia da polimerase e do isolamento bacteriológico convencional na detecção de Salmonella Dublin em amostras de fezes de bezerros infectados experimentalmente
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Silva,D.G.; Silva,D.R.; Silva,P.R.L.; Cícero,E.A.S.; Ferraz,A.L.J.; Lemos,M.V.F.; Fagliari,J.J.
The efficiency of the Polymerase Chain Reaction (PCR) combined with selective enrichment broth was compared with the standard microbiological techniques for detection of Salmonella Dublin in fecal samples of 10 to 15-days-old Holstein calves, experimentally infected with 10(8) CFU of Salmonella Dublin. Seventy-six fecal samples were analyzed using PCR associated with selenite cystine (SC) and Muller-Kauffmann tetrathionate (TMK) broths and standard microbiological techniques. Regardless of the selective enrichment broth used, the standard microbiological techniques were significantly better than PCR in detection of positive samples of Salmonella Dublin. However, the simultaneous use of both techniques provided detection of a larger number of positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calves; Feces; Salmonella Dublin; PCR; Culture.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000300035

Wolbachia spp. interfere na detecção molecular de Ehrlichia canis e Anaplasma platys em cães com microfilaremia
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Xavier,M.S.; Fonseca,C.N.; Ferreira,R.F.; Castro,T.X.; Neves,F.P.G.; Barbosa,A.V.; Cerqueira,A.M.F.; Almosny,N.R.P.
This report aimed to study the interference in molecular testing for Ehrlichia canis and Anaplasma platys in blood of 155 dogs from the coastal region of Rio de Janeiro. Five Anaplasmataceae positive samples but negative for E. canis and A. platys, from microfilaremic animals, were chosen for sequencing. These sequences, when compared to Gen et Bank database, showed 88% to 100% similarity with Wolbachia spp. denoting an interference in the detection of DNA from other members of Anaplasmataceae, possibly due to a high concentration of Wolbachia spp. DNA.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cão; Erlichiose; Sequenciamento; Microfilarêmico; PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000401197

Detecção de Mycoplasma spp. e Ureaplasma diversum em vacas com distúrbios reprodutivos
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Buzinhani,M.; Metiffogo,E.; Timenetsky,J.
Foram utilizadas 112 amostras de muco vulvovaginal, coletados de vacas com distúrbios reprodutivos, para pesquisa de Mycoplasma e Ureaplasma. Para isolamentos, foram usados meios específicos para micoplasmas (SP-4) e para ureaplasmas. PCR genérica, PCR específica para Mycoplasma bovis e nested-PCR em tubo único para Ureaplasma diversum foram realizados com os DNAs extraídos das amostras. Mycoplasma spp. e U. diversum foram detectados em 12,5 e 25,0%, respectivamente. A PCR genérica resultou em reações positivas em 63,4% das amostras transportadas em SP-4 e em 69,6% das transportadas em meio de ureaplasma. M. bovis foi detectado, na PCR específica, em 9,8% das amostras e U. diversum, na nested-PCR, em 37,5%. Houve maior sensibilidade na metodologia da PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaca; Mycoplasma bovis; Ureaplasma diversum; Cultura; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000600003

Toxoplasma gondii in milk of naturally infected dairy ewes on west mesoregion of Santa Catarina state, Brazil
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Ossani,R.A.; Borges,H.A.T.; Souza,A.P.; Sartor,A.A.; Miletti,L.C.; Federle,M.; Moura,A.B.
ABSTRACT This study aimed to detect Toxoplasma gondii in the milk of dairy sheep in the Western mesorregion of state of Santa Catarina by bioassay (22 milk samples from eight ewes seropositive; IFA ≥256) and PCR [for the detection of agent in the brains of mice inoculated on bioassay and directly from milk (108 samples from 42 seropositive ewes (IFA, ≥64) in different lactation periods)]. T. gondii DNA was detected in mice brains inoculated with milk from eight sheep (a sample of the 45th day of lactation and seven in the collection of 90th day) and directly from the milk in samples of the second collection (90 days) in five animals. Taking into account both assays, from a total of 42 ewes in lactation and seropositive for T. gondii, 30.95% (13/42) of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Milk sheep; PCR; Santa Catarina state.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501294

Presença do genoma de BoHV-5 no líquido cefalorraquidiano de bovinos com meningoencefalite herpética
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Massitel,J.L.; Wesgueber,J.; Oliveira,R.A.M.; Queiroz,G.R.; Fritzen,J.T.T.; Alfieri,A.A.; Lisbôa,J.A.N.
The diagnosis of bovine herpesvirus 5 (BoHV-5) encephalitis is confirmed after death by laboratory methods applied to brain fragments. Alternative methods to confirm ante-mortem diagnosis are important because the disease is not always lethal. The aim of this study was to investigate whether the presence of the virus genome in the cerebrospinal fluid (CSF) detected by polymerase chain reaction (PCR) might be admitted as a method for ante-mortem diagnosis. CSF samples were taken from 14 animals suffering from BoHV-5 encephalitis, diagnosed by characteristic histopathological lesions in the brain and by identification of the virus genome by PCR in different portions of the brain. Virus DNA was detected in the CSF of 21.42% (3/14) of the evaluated animals....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Herpesvírus; Diagnóstico; PCR; Líquor.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200548

Elevada frequência de Aeromonas spp. e genes de virulência em cultivos de tilápia-do-nilo (Oreochromis niloticus) em tanques-rede, na região semiárida de Pernambuco, Brasil
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Kim,F.J.P.; Silva,A.E.M; Silva,R.V.S.; Kim,P.C.P.; Acosta,A.C.; Silva,S.M.B.C.; Sena,M.J.; Mota,R.A.
RESUMO Objetivou-se avaliar a ocorrência de Aeromonas spp. em peixes e amostras de água na região semiárida de Pernambuco e avaliar a frequência de aerolissina (aerA), enterotoxina citotóxica (act), enterotoxina citotônica (alt) e serina protease (ahp) nesses isolados. Foram analisados 70 peixes vivos e oito mortos com sinais clínicos de aeromoniose e 16 amostras de água. Aeromonas spp. foram identificadas por análises microbiológicas (provas bioquímicas) e molecular, usando-se primers específicos para a região 16S rRNA, e a distribuição dos quatro fatores de virulência (aerA, alt, act e ahp) foi investigada por ensaio de PCR. Cento e cinquenta e cinco (84,7%) isolados foram confirmados como Aeromonas spp. na análise molecular. Os genes de virulência mais...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aeromoniose; Fatores de virulência; Oreochromis niloticus; PCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000501609

Ocorrência do subtipo B do vírus da imunodeficiência felina em gatos domésticos da região sul do estado do Rio Grande do Sul, Brasil
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Silva,F.S.; Castro,C.C.; Finger,P.F.; Silva,D.S.; Taniwaki,S.A.; Ullmann,L.S.; Fischer,G.; Vargas,G.D.; Lima,M.; Araújo Jr,J.P.; Hübner,S.O.
No Brasil existem poucos estudos sobre a ocorrência da infecção pelo vírus da imunodeficiência felina (FIV), assim como a determinação dos subtipos circulantes, o que é indispensável para o desenvolvimento de vacinas e novos testes diagnósticos. O presente trabalho investigou a ocorrência da infecção pelo FIV entre os anos de 2010 e 2011 em gatos domésticos submetidos a atendimento clínico na cidade de Pelotas e região. Amostras de sangue total de 70 animais, incluindo suspeitos (28) ou não suspeitos (42) da infecção pelo FIV, foram submetidas à reação de PCR nested. Os resultados indicaram uma frequência de infecção de 15,7% (11/70) e a análise dos fatores associados (sexo, idade e condição clínica) evidenciou uma maior ocorrência em gatos com idade...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gato; Vírus da imunodeficiência felina; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000100001

Padronização de uma PCR para diagnóstico molecular de Microsporum canis em amostras de pelos e crostas de cães e gatos
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Leal,C.A.S.; Silva,G.G.; Silva,G.M.; Silva,L.B.G.; Pinheiro Júnior,J.W.; Mota,R.A.
RESUMO Objetivou-se neste estudo padronizar um protocolo de reação em cadeia da polimerase (PCR) para detecção de Microsporum canis em amostras de pelos e/ou crostas de cães e gatos. Foram selecionadas 48 amostras previamente identificadas por meio de cultura. Destas, 23 foram positivas para dermatófitos no cultivo. Padronizou-se a PCR a partir de primers desenhados para o alvo M. canis. Sessenta e um por cento (14/23) das amostras positivas para dermatófitos foram identificadas como M. canis em cultura. Desse total, 71,4% (10/14) apresentaram um fragmento de 218pb compatível com o esperado para a espécie fúngica alvo dessa reação. Observou-se uma sensibilidade de 71,4% e especificidade de 100% na PCR, além de uma boa concordância entre essas técnicas de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cães; Gatos; Dermatófitos; PCR; Pelos.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000401143

Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Souza,D.M.B.; Barros,M.G.O.; Silva,J.S.C.; Silva,M.B.; Coleto,Z.F.; Jimenez,G.C.; Adrião,M.; Wischral,A.
Fifteen female canines with mammary tumors and 6 normal females were used to study mutations in exons 4 to 8 of the p53 gene. DNA samples from the tumors, respective adjacent normal mammary tissue and mammary glands from healthy animals were sequenced and analyzed for the presence of mutations. Mutations were found in 71.8% of the samples and the most frequent were missense mutations. The most attacked exons in the mammary tumor were 5, 7 and 8, with 23.4, 31.6 and 23.4% mutations, respectively. Canine mammary tumors are related to mutations in gene p53 and mutations mostly occur in the region of the protein that is linked to the DNA in the cell nucleus, which can change the functionality of the cell and propitiate tumor growth. Despite being...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bitch; DNA; Mamma; Molecular biology; Neoplasm; PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200013

Identificação de Mycobacterium bovis em carcaças de bovinos abatidos no estado da Bahia, Brasil, por métodos bacteriológico e molecular
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Alzamora Filho,F.; Reis,V.M.; Fehlberg,I.; Alcântara,A.C. de; Cavalcante,M.P.; Rocha,V.C.F.; Costa,J.N.
O objetivo do presente trabalho foi utilizar métodos bacteriológicos e moleculares para a identificação do Mycobacteriumbovis em lesões observadas em carcaças de bovinos durante a inspeção post mortem de rotina em matadouros-frigoríficos com serviço de inspeção oficial. Foram acompanhados o abate e a inspeção de 825.394 bovinos, sadios, ao exame antemortem pelo serviço de inspeção oficial em 10 matadouros-frigoríficos do estado da Bahia, entre abril de 2009 e abril de 2012. Cento e oitenta bovinos apresentaram lesões sugestivas de tuberculose e outras linfadenites, as quais foram avaliadas quanto à presença de Mycobacterium bovis por exame bacteriológico e pela PCR multiplex. A maioria das lesões estava localizada em linfonodos do trato respiratório e 71%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium bovis; PCR; Bacteriologia; BAAR; Bovino.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501585

Caracterização bioquímica e molecular de Cryptococcus spp. isolados de excretas ambientais de pombos (Columba livia domestica)
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Colombo,G.; Táparo,C.V.; Araújo Júnior,E.C.; Makatu,M.Y.; Santos,F.S.; Marinho,M.
Os pombos possuem uma rica diversidade de microrganismo, entre eles fungos sapróbios, como do gênero Cryptococcus, que podem atuar como agentes patogênicos para o homem e animais. Objetivou-se o isolamento, a caracterização bioquímica e a molecular de amostras de Cryptococcus spp. de excretas ambientas de pombos. Foram colhidas 100 amostras ambientais de pontos equidistantes e representativos da área da cidade de Araçatuba, São Paulo. As amostras foram rasteladas do solo de vias públicas, armazenadas em frasco coletor e encaminhadas para o Laboratório de Bacteriologia e Micologia da FMVA, onde foram processadas e cultivadas em duplicata, em placas de Petri contendo ágar Sabouraud dextrose a 4% e Niger. Em seguida, foram incubadas à temperatura ambiente e a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptococcus neoformans; Pombos; Bioquímico; PCR; Saúde pública.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601639

PCR Multiplex para detecção dos principais herpesvírus neurológicos de ruminantes
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Fonseca Jr.,A.A.; Costa,E.A.; Oliveira,T.S.; Sales,E.B.; Sales,M.L.; Leite,R.C.; Heneimann,M. B.; Reis,J.K.P.
Desenvolveu-se uma PCR multiplex (mPCR) para diagnóstico diferencial de encefalite bovina causada por herpesvírus suíno 1 (SuHV-1), herpesvírus bovino 1 (BoHV-1), herpesvírus bovino 5 (BoHV-5) e herpesvírus ovino 2 (OvHV-2). Os iniciadores foram projetados após alinhamento de sequências disponíveis no banco de genomas (GenBank) e a reação foi padronizada levando-se em consideração a concentração dos reagentes e os tipos diferentes de DNA polimerase. Após determinação da especificidade e sensibilidade, 65 amostras de encéfalo de bovinos com síndrome neurológica foram submetidas à análise. A sensibilidade analítica para detecção de BoHV-1, BoHV-5 e SuHV-1 foi, respectivamente, 10(1,2) TCID50/50µL, 10(1,0) TCID50/50µL, 10(1,3) TCID50/50µL na reação multiplex....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Encefalite; Herpesvírus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600018

Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Carvalho,R.; Passos,L.M.F.; Oliveira,A.M.; Henry,M.; Martins,A.S.
The genome of one equine embryo and three equine semen specimens collected from a Brazilian farm were tested by polymerase chain reaction (PCR) for the presence of EHV-1 and EHV-4-specific timidine kinase (TK) sequences. The PCR detected specific EHV-1 TK gene sequences in all samples tested. The peripheral blood leukocytes (PBL) of the embryo donor mare also was amplified by EHV-1 TK primers. Infectious virus was not recovered from any specimens. The animals did not show any clinical signal of EHV-1 or EHV-4 infections. EHV-4 was not detected in the studied specimens. The results indicate that PCR was more sensitive than virus isolation in cell culture for detecting EHV-1 in semen of carrier horses.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine; Semen; Embryo; EHV-1; EHV-4; PCR.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400002

Associação à positividade da Lawsonia intracellularis com a expressão clínico-patológica da infecção em suínos da região metropolitana de Bucaramanga (Santander, Colômbia)
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Jiménez,A.P.; Duarte,L.Z.; Cortez,L.S.; Granados,M.; Florez,C.; Villamizar,A.; Parra-Martin,J.A.
RESUMO Porções de íleo terminal foram coletados de 100 suínos com sinais de doença gastrointestinal na área metropolitana de Bucaramanga, a fim de se estudar a eficiência do diagnóstico de enteropatia proliferativa suína (PPE) pela técnica de PCR aninha (PCRa) empregando sequências específicas (primers) para L. intracellularis: 16S ARN região (270pb) e sua correlação com achados clínicos e patológicos. Todas as amostras foram processadas para se determinar a associação entre positividade por PCR, os sinais clínicos, os achados de necropsia e as lesões histológicas. Cinquenta e seis por cento das amostras foram positivas para L. intracellularis pela PCRa. Só 2% exibiram resultados positivos pela técnica Warthin-Starry. Trinta e um de 100 animais com sinais...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ileíte suína; PCR; Warthin-Starry.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100181

Avaliação in vitro da atividade inibitória de Lactobacillus spp., isolados do inglúvio e cecos de aves sobre Salmonella
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Barros,M.R.; Andreatti Filho,R.L.; Lima,E.T.; Crocci,J.A.
Inglúvios e cecos de reprodutoras comerciais de frangos de corte foram utilizados para o isolamento de Lactobacillus spp. As estirpes isoladas foram caracterizadas como Gram-positivo, catalase negativo, produtoras de gás em glicose, não produtoras de H2S em triple sugar iron e identificadas pela reação em cadeia da polimerase como Lactobacillus reuteri e Lactobacillus salivarius. A utilização da técnica spot-on-the-lawn para avaliação da inibição in vitro permitiu a análise de vários microrganismos simultaneamente. Todas as estirpes isoladas inibiram in vitro S. Enteritidis fagotipo 4, S. Enteritidis fagotipo 28, S. Typhimurium, S. Pullorum, S. Agona, S. Anatum, S. Dublin e S. Senftenberg.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reprodutora de frango de corte; Identificação; Lactobacillus; Salmonella; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000400013

In vitro culture, PCR , and nested PCR for the detection of Theileria equi in horses submitted to exercise
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Baldani,C.D.; Canola,P.A.; Neto,J.C.L.; Machado,R.Z.
This study compared the usefulness of in vitro culture, PCR, and nested PCR for the diagnosis of Theileria equi in horses submitted to stress during exercise. Blood samples from 15 apparently healthy horses, previously conditioned to a high-speed equine treadmill, were taken prior to and after exercise. The animals were divided into two experimental groups: 30-day training schedule (G1) and 90-day training schedule (G2). Statistical analysis was performed using a chi-square test and kappa statistic was used in order to assess agreement. No significant difference was observed between samples collected at resting or after exercise. In G1, merozoites of T. equi were detected in the blood smears of four horses before in vitro culture, whereas 14 samples were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eqüino; Theileria equi; Diagnóstico; Cultivo in vitro; PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300005

A VP3/VP1 gene polymerase chain reaction assay for detection of chicken anemia virus in broiler samples
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Nogueira,E.O.; Brentano,L.; Ferreira,A.J.P.
A PCR assay was designed for amplification of the highly conserved VP3 gene and a 5' region of the VP1 gene, for the diagnosis of CAV in organ samples of broiler flocks suspected of chicken infectious anemia. A comparison of the VP3/VP1 PCR with in vivo virus isolation revealed 100% agreement of the results, with 13 positive and 3 negative samples in both assays, indicating that the VP3/VP1 PCR is a specific diagnostic method. Tissues from additional 24 broiler chicken flocks, with CAV-like lesions and clinical history were then tested only by the VP3/VP1 PCR and a reference PCR with published primers for the VP1 gene. Nineteen samples resulted positive and one negative in both PCR, while another 4 samples were positive only in the VP3/VP1 PCR. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Chicken anemia virus; CAV; PCR; Diagnostic test.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000800001

Ocorrência do vírus da imunodeficiência felina e do vírus da leucemia felina em gatos domésticos mantidos em abrigos no município de Belo Horizonte
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Teixeira,B.M.; Rajão,D.S.; Haddad,J.P.A.; Leite,R.C.; Reis,J.K.P.
Investigou-se a ocorrência da infecção pelo vírus da imunodeficiência felina (FIV) e pelo vírus da leucemia felina (FeLV) em gatos domésticos, provenientes de dois abrigos, no município de Belo Horizonte. Amostras de sangue de 145 animais foram coletadas e testadas para detecção do FIV pela reação em cadeia da polimerase (PCR). Destas amostras, 40 foram testadas para o antígeno p26 de FeLV por meio de ELISA. Observaram-se duas fêmeas (1,4%) e quatro machos (2,8%) positivos para FIV e nove fêmeas (22,5%) e quatro machos (10,0%) positivos para FeLV.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gato; Vírus da imunodeficiência felina; Vírus da leucemia felina; PCR; ELISA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000400019

Identification of equine herpesvirus type 1 as cause of abortion in mares in Southern Brazil
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Estima-Silva,P.; Riet-Correa,F.; Coelho,A.C.B.; Echenique,J.V.Z.; Marcolongo-Pereira,C.; Lima,M.; Diel,D.G.; Schild,A.L.
RESUMO Foi realizado um estudo retrospectivo dos casos suspeitos de aborto por herpesvírus equino observados na região sul do Rio Grande do Sul entre 1978 e 2016. Foram revisados os protocolos de necropsia do Laboratório Regional de Diagnóstico da Faculdade de Veterinária da Universidade Federal de Pelotas resgatando-se os dados epidemiológicos, lesões macroscópicas e histológicas de cada caso. Foram observados dois surtos da enfermidade com prevalência entre 5,7% e 50% nos diferentes estabelecimentos, e dois casos individuais. Em todos os casos foram enviados fragmentos de órgãos fetais formolizados. Histologicamente, em todos os casos foram observados focos de necrose no fígado, pulmão e baço e presença de corpúsculos de inclusão acidofílico em...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Abortion; Equine herpesvirus-1; Immunohistochemistry; PCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000401421

Infecção em cão por Brucella abortus: relato de caso
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Megid,J.; Salgado,V.R.; Keid,L.B.; Siqueira,A.K.; Meirelles,C.E.; Moretti,D.M.
Brucella abortus infection is reported in a dog from a rural area that presented at clinical evaluation left testicular enlargement and right testicular decrease. Serum resulted negative to rapid agglutination test and agar gel immunodifusion with Brucella ovis antigen but positive to buffered plate agglutination test, tube agglutination test and 2- Mercapthoetanol with B. abortus antigen. Brucella isolation was negative in blood, testicular material, semen and urine. Brucella DNA was detected in PCR from urine and blood.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cão; Orquite; Brucella abortus; Sorologia; Microbiologia; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000600036

Comparação entre método bioquímico e reação em cadeia de polimerase para identificação de Lactobacillus spp., isolados de aves
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Barros,M.R.; Andreatti Filho,R.L.; Oliveira,D.E.; Lima,E.T.; Crocci,A.J.
Lactobacilos foram isolados do inglúvio e cecos de reprodutoras pesadas e caracterizados como Gram-positivo, catalase negativo, produtores de gás em glicose e não produtores de H2S em triple sugar iron e pela fermentação de carboidratos. Utilizaram-se os iniciadores: Lac 1/23-10C para detecção de Lactobacillus acidophilus, L. crispatus, L. amylovorus, L. gasseri, L. helveticus e L. jensenii; Lac 2/LU-1' para L. acidophilus; Fer 3/Fer 4 para L. fermentum; Reu 1/Reu 2 para L. reuteri e Sal 1 e Sal 2 para L. salivarius. L. reuteri e L. salivarius foram identificados pela reação em cadeia de polimerase (PCR) e pelo teste bioquímico, enquanto L. acidophilus, L. fermentum e Lactobacillus sp. somente pelo teste bioquímico. Os resultados obtidos na PCR foram mais...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galinha; Reprodutora de frango de corte; Identificação bioquímica; Lactobacillus; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000200006

Análise de polimorfismo do gene da somatotropina em vacas Nelore e seu efeito sobre o peso à desmama de suas progênies
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Faria,F.J.C.; Guimarães,S.E.F.; Lima,R.M.G.; Mourão,G.B.; Pinheiro,L.E.L.
Informações sobre peso à desmama de um rebanho Nelore foram utilizadas após ajuste para idade padrão de 205 dias, sexo da cria, idade da mãe, touro e mês de desmama, para separar as reprodutrizes em dois grupos, cujos filhos diferiam nesse peso. As médias ajustadas pelo método dos quadrados mínimos foram para os grupos pesado (P) e leve (L) de 163,21± 2,18kg e 134,44± 2,18kg, respectivamente, com 41 animais em cada grupo. Essas reprodutrizes foram submetidas à coleta de sangue para estudo de polimorfismos do gene da somatotropina bovina, pela técnica de PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). A amplificação de uma região entre o éxon III e V do gene da somatotropina permitiu analisar dois sítios de restrição. Para o...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Somatotropina; PCR; RFLP; Polimorfismo genético.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000600011

Utilização de parte da região codificadora da glicoproteína b na diferenciação do herpesvírus bovino 1.1, herpesvírus bovino 1.2 e herpesvírus bovino 5
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Costa,E.A.; Barbosa-Stancioli,E.F.; Leite,R.C.; Oliveira,G.D.R.; Rocha,M.A.
Utilizou-se uma reação em cadeia de polimerase (PCR) previamente desenvolvida para a amplificação de parte da região única longa 27 (UL27) do genoma de herpesvírus bovino 1.1 (BoHV-1), que codifica a glicoproteína B, buscando a diferenciação entre isolados de BoHV-1.1 e BoHV-5. Os produtos de PCR gerados a partir de isolados de BoHV-1.1 e BoHV-5 mostraram padrão de amplificação diferenciado em seus tamanhos moleculares. Analisando as seqüências de nucleotídeos dos produtos de PCR obtidos de dois isolados de BoHV-5, juntamente com as seqüências dos produtos de PCR obtidos de um isolado de BoHV-1.1 e de três isolados de BoHV-1.2, previamente depositados no GenBank, verificou-se que a diferença observada na amplificação se deve ao número de repetições de G-C...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-1.1; BoHV-1.2; BoHV-5; GB; PCR; Seqüências-motivo.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000200001

Detecção da infecção pelo vírus da artrite-encefalite caprina: imunodifusão em ágar e reação em cadeia da polimerase com "primers" degenerados
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Rutkoski,J.K.; Werenicz,R.; Reischak,D.; Wendelstein,A.C.; Moojen,V.; Ravazzolo,A.P.
O objetivo deste trabalho foi analisar amostras de soro e de células sangüíneas de caprinos para detecção de anticorpos e DNA proviral do vírus da artrite-encefalite caprina (CAEV), respectivamente. Utilizou-se a técnica de imunodifusão em ágar (AGID) e a reação em cadeia da polimerase (PCR) com "primers" degenerados. Foram analisadas amostras de diferentes procedências: 39 de Mato Grosso do Sul (MS), 19 de São Paulo (SP) e 22 do Ceará (CE), dessas últimas, 12 oriundas de animais importados do Canadá. Os resultados de AGID e PCR foram discordantes, pois o primeiro permitiu a detecção de 25 animais soropositivos, enquanto a PCR detectou DNA proviral de CAEV em 16 amostras. Pela PCR foi possível identificar animais infectados cujos testes sorológicos foram...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vírus da artrite-encefalite caprina; CAEV; PCR; "primers" degenerados; AGID.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000600001

Infecção sistêmica por Salmonella Typhimurium em papagaio-verdadeiro (Amazona aestiva)
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Souza,M.L.; Azuaga,L.B.S.; Coutinho Netto,C.R.M.; Gomes,D.C.; Ramos,C.A.N.; Leal,C.R.B.
ABSTRACT The maintenance of wild animals in captivity can be a source for transmission of infectious and zoonotic diseases. In February 2016, blue-fronted amazon parrots that were kept at the Centro de Reabilitação de Animais Silvestres (CRAS) of Campo Grande - MS died suddenly. The specimens were sent to the Universidade Federal de Mato Grosso do Sul (UFMS), Faculdade de Medicina Veterinária e Zootecnia (FAMEZ) to be necropsied. Anatomopathological exams were performed and organ fragments from all systems were collected for histopathological exams. Liver and spleen fragments were also collected for bacteriological exams. Histopathological exam revealed bacterial aggregates distributed through the organs. Bacteriological exam isolated Salmonella spp. from...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: PCR; Psittaciformes; Salmonelose.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000200637

Detecção dos genes de Staphylococcus aureus, enterotoxinas e de resistência à meticilina em leite
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Dias,N.L.; Silva,D.C.B.; Oliveira,D.C.B.S.; Fonseca Junior,A.A.; Sales,M.L.; Silva,N.
Realizou-se a detecção do gene de Staphylococcus aureus, de enterotoxinas e de resistência à meticilina com extração de DNA feita diretamente de amostras de leite. Das 200 amostras estudadas, 145 (72,5%) amplificaram o gene femA, e estas foram analisadas quanto à presença dos genes sea, seb, sec e mecA. Os genes das enterotoxinas mais prevalentes foram: sea (60%), seb (37,9%) e sec (6,9%). Foram encontradas 18 amostras de leite (11,0 %) com S. aureus portadores do gene mecA. A detecção de S. aureus diretamente do leite, sem a necessidade de isolamento bacteriano e a caracterização do potencial enterotoxigênico, demonstra que a técnica de PCR é muito útil para estudos epidemiológicos das infecções estafilocócicas da glândula mamária. O alto percentual...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Enterotoxinas; MRSA; PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600036

Aspectos clínicos e terapêuticos de infecção por Mycobacterium bovis em gato doméstico - relato de caso
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Alves,A.S.; Araújo,S.B.; Ferreira,J.S.; Medeiros,L.K.G.; Oliveira,E.L.; Dantas,A.F.M.; Souza,A.P.
RESUMO Este artigo tem como objetivo relatar um caso de infecção por Mycobacterium bovis em um gato doméstico, com seis meses de idade, enfatizando seus aspectos clínicos e terapêuticos. O felino apresentava como histórico a ingestão de leite de vaca não fervido e posteriormente desenvolveu sinais de anorexia, tosse não produtiva e linfadenomegalia. Para elucidação do diagnóstico, foram realizados exames complementares de imagem, hematológicos, citológicos e reação em cadeia da polimerase (PCR). Diante da confirmação de infecção por Mycobacterium, o tratamento foi instituído por meio da associação de três antimicrobianos (enrofloxacino 5mg/kg, claritromicina 10mg/kg e rifampicina 15mg/kg), por via oral, a cada 12 horas, aos quais o animal respondeu...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobianos; Diagnóstico; Felino; PCR; Tuberculose.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601896

Eficácia do dipropionato de imidocarb, da enrofloxacina e do cloridrato de oxitetraciclina no tratamento de bovinos naturalmente infectados por Anaplasma marginale
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Alberton,L.R.; Orlandini,C.F.; Zampieri,T.M.; Nakamura,A.Y.; Gonçalves,D.D.; Piau Júnior,R.; Zaniolo,M.M.; Cardim,S.T.; Vidotto,O.; Garcia,J.L.
O agente de maior importância, em relação à anaplasmose bovina, é o Anaplasma marginale. Os principais sinais clínicos dessa enfermidade são anemia hemolítica, icterícia, dispneia, taquicardia, febre, fadiga, lacrimejamento, sialorreia, micção frequente, anorexia, perda de peso, aborto e morte. A terapia antimicrobiana é o principal protocolo terapêutico. O objetivo do presente trabalho foi avaliar a eficácia do dipropionato de imidocarb, da enrofloxacina e do cloridrato de oxitetraciclina no tratamento de bovinos leiteiros naturalmente infectados por Anaplasma marginale. Para isso, foram avaliados 48 zebuínos mestiços que apresentavam os sinais clínicos sugestivos da doença. Os animais foram submetidos à coleta de sangue para a realização de hemograma e à...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anaplasmaspp.; Bovídeos; PCR; Terapêutica.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000401056

Diagnosis of Brucella ovis infection by serology and PCR in urine samples from naturally infected rams in the state of Piauí
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Costa,E.A.; Sant'Ana,F.M; Carvalho,C.J.S.; Moustacas,V.S.; Silva,S.M.M.S.; Paixão,T.A.; Santos,R.L.
A brucelose ovina causada pela Brucella ovis é uma doença reprodutiva de carneiros caracterizada por epididimite, orquite, com consequente diminuição da fertilidade e prejuízos econômicos significativos. O presente trabalho teve por objetivo avaliar a aplicabilidade da técnica de PCR como um método de diagnóstico em campo, comparado-a com a técnica de IDGA. Foram coletadas amostras de urina e soro de 90 carneiros oriundos de 31 rebanhos localizados no Estado do Piauí. Quatro das 31 (12,9%) propriedades avaliadas apresentaram animais positivos. Dezoito (20%) amostras de urina foram positivas pela PCR, enquanto o método de IDGA identificou 16 (17,8%) carneiros soropositivos. Embora os métodos tenham apresentado concordância baixa na estatística Kappa...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucella ovis; Sheep; Diagnosis; PCR; AGID.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300029

Chronic Chagas disease: PCR-xenodiagnosis without previous microscopic observation is a useful tool to detect viable Trypanosoma cruzi
Provedor de dados: Biological Research
Autores: Saavedra,Miguel; Zulantay,Inés; Apt,Werner; Martínez,Gabriela; Rojas,Antonio; Rodríguez,Jorge.
We evaluate the elimination of the microscopic stage of conventional xenodiagnosis (XD) to optimize the parasitological diagnosis of Trypanosoma cruzi in chronic Chagas disease. To this purpose we applied under informed consent two XD cages to 150 Chilean chronic chagasic patients. The fecal samples (FS) of the triatomines at 30, 60 and 90 days post feeding were divided into two parts: in one a microscopic search for mobile trypomastigote and/or epimastigote forms was performed. In the other part, DNA extraction-purification for PCR directed to the conserved region of kDNA minicircles of trypanosomes (PCR-XD), without previous microscopic observation was done. An XD was considered positive when at least one mobile T. cruzi parasite in any one of three...
Tipo: Journal article
Palavras-chave: Chronic Chagas disease; PCR; Triatoma infestans; Trypanosoma cruzi; Xenodiagnosis.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300011

Identificação de genes cry de Bacillus thuringiensis no controle de Sphenophorus levis, o bicudo da cana-de-açúcar
Provedor de dados: Bragantia
Autores: Cícero,Elaine Aparecida Silva; Ferraudo,Antonio Sergio; Lemos,Manoel Victor Franco.
A bactéria B. thuringiensis caracteriza-se pela produção de proteínas tóxicas a representantes de diversas ordens de insetos, as quais são codificadas por genes cry. Este trabalho foi realizado com objetivo de selecionar isolados de B. thuringiensis, por meio da caracterização morfológica e molecular, identificando as diferentes subclasses dos genes cry3 e cry35 e determinar a patogenicidade contra Sphenophorus levis, uma das mais importantes pragas da cultura da cana-de-açúcar. Foram utilizados 1163 isolados de B. thuringiensis e com a observação em microscópio com contraste de fases foram confirmadas como pertencentes à espécie de B. thuringiensis. O material genético foi purificado pela matriz de troca iônica "Instagene Matrix" e submetido a PCR com...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Bioensaio; Controle biológico.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052009000400001

Molecular systematics of the Phyllachorales (ascomycota, fungi) based on 18S ribosomal DNA sequences
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Wanderlei-Silva,Denise; Ramalho Neto,Eduardo; Hanlin,Richard.
In order to evaluate the monophyly of the Phyllachorales from a molecular standpoint and elucidate its phylogenetic relationships with other orders, a segment of the 18S rRNA gene from several representatives of the Phyllachorales, including species of Glomerella, Phyllachora, Coccodiella (=Coccostroma), Sphaerodothis, Ophiodothella, as well as Magnaporthe was sequenced. Maximum Parsimony analysis revealed that the Phyllachorales was a polyphyletic assemblage of taxa. None of the other members of the Phyllachorales, which produced either a clypeus or stroma, clustered with Glomerella. Of the taxa examined, was Coccodiella the closest relative of Phyllachora. Magnaporthe was closely related to the Diaporthales. Our 18S rDNA data highly supported Glomerella...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA sequencing; 18S ribosomal DNA; PCR; Phyllachorales.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300002

Polymerase chain reaction (PCR) identification of terverticillate Penicillium species isolated from agricultural soils in eskişehir province
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Demirel,Rasime; Sariozlu,Nalan Yilmaz; İlhan,Semra.
In the present study, nine terverticillate Penicillium isolates (P. griseofulfum, P. puberulum, P. crustosum, P. aurantiogriseum, P. chrysogenum, P. primulinum, P. expansum, P. viridicatum, Eupenicillium egyptiacum) from 56 soil samples were characterized genetically by a PCR method. The DNAs of the strains were isolated using the glass beads and vortexing extraction method and then used for PCR amplification with the internal transcribed spacer 1 (ITS1) and ITS4 universal fungal specific primers. The ITS regions of fungal ribosomal DNA (rDNA) were sequenced through the CEQ 8000 Genetic Analysis System. ITS-5.8S sequences obtained were compared with those deposited in the GenBank Database. The results indicated that the identification of Penicillium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicillium; Identification methods; PCR; ITS.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600013

Detection of Lsr2 gene of Mycobacterium leprae in nasal mucus
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Custodio,Luiz Antonio; Saito,Alexandre; Amarante,Marla Karine; Fujita,Thiago Cezar; Perim,Aparecida de Lourdes; Costa,Ivete Conchon; Felipe,Ionice; Jankevicius,Shiduca Itow.
In the present study, nasal mucus from patients with leprosy were analyzed by PCR using specific primers for Lsr2 gene of Mycobacterium leprae. The presence of Lsr2 gene in the nasal mucus was detected in 25.80% of patients with paucibacillari leprosy, and 23.07% of contacts. Despite the absence of clinical features in the contact individuals, it was possible to detect the presence of Lsr2 gene in the nasal mucus of these individuals. Therefore, PCR detection of M. leprae targeting Lsr2 gene using nasal mucus samples could contribute to early diagnosis of leprosy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium leprae; Leprosy; Hanseniase; Nasal mucus; PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300007

High quality DNA from human papillomavirus (HPV) for PCR/RFLPs
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Wanderlei-Silva,Denise; Nobre,Mariana; Gonzaga,Rosa Silva; Viana,Luciana Silva; Ramalho Neto,Eduardo.
The analysis of DNA in clinical samples for a secure diagnostic has become indispensable nowadays. Techniques approaching isolation of high molecular weigth DNA of HPV could lead to efficient amplification and early clinical diagnosis of the virus DNA by PCR (polymerase chain reaction). We describe a fast, non-toxical, efficient and cheap method for DNA isolation of human papilloma virus (HPV) from cervical smears using guanidine (DNAzol solution). A 450 bp DNA band correponding to the late region (L1) of the virus genome was detected by PCR, showing that the DNAzol extraction soluction generated a good viral DNA yield. The electrophoretic pattern after digestion with restriction endonucleases (RFLPs/PCR) revealed the predominance of HPV-16 and HPV-33 in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; DNA purification; PCR; RFLP.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100006

Incidence of group A rotavirus in urban and rural areas of the city of Londrina-Brazil, from 1995 to 1997
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Meneghetti,Ângelo Cesar; Bolognini,Andrea Maria; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Santos,Norma; Nozawa,Carlos Mitihiko.
Rotaviruses are common pathogens and the causal agents of acute diarrhea among children and young animals. The involvement of rotavirus in human diarrheal disease among population of urban and rural areas of the city of Londrina, Parana was evaluated. Nine hundred and five fecal specimens from persons with diarrhea were studied, being 686 and 219 from urban and rural areas, respectively. Thirty-eight samples (4,2%) were positive for rotavirus by polyacrylamide gel electrophoresis of viral RNA and latex agglutination test of which 36 were from urban and two from rural areas. Out of the positive specimens, 17 strains were further characterized by RT-PCR typing assay, resulting in 16 strains of G1 genotype while one sample was found to be a mixture of G1 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Diarrhea; PAGE; LA; PCR.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300006

Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers Analyses
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Deef,Lamiaa Elsayed Mokhtar.
ABSTRACT  Quail is an important and interesting group of galliform birds. The Common quail (Coturnix coturnix); the Japanese quail (Coturnix japonica); the Panda quail (Coturnix japonica); the Dotted white quail (Coturnix japonica) and the Bobwhite quail (Colinus virginianus) were used in this study. PCR-RFLP and SDS-proteins were performed to reveal the genetic characterization and genetic relationship of the studied quails. Analysis of fragments generated by digestion of PCR product with restriction enzyme NlaIII recorded highly polymorphic restriction profiles. There is a wide intraspecific COI, SEMA3E and TLX genes variability among the studied quails. Protein bands varied from10 to 18 between quails with minimum number of bands were in the Dotted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birds; PCR; Quail; SDS-PAGE; Galliformes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100425

Validation of a PCR Assay for Chlamydophila abortus rRNA gene detection in a murine model
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Silva-Zacarias,Francielle Gibson da; Alfieri,Amauri Alcindo; Spohr,Kledir Anderson Hofstaetter; Lima,Bruna Azevedo de Carvalho; Negrão,Fábio Juliano; Lunardi,Michele; Freitas,Julio Cesar de.
Chlamydophila abortus (C. abortus) is associated with reproductive problems in cattle, sheep, and goats. Diagnosis of C. abortus using embryonated chicken eggs or immortalized cell lines has a very low sensitivity. Polymerase chain reaction (PCR) assays have been used to detect C. abortus infection in clinical specimens and organ fragments, such as placenta, fetal organs, vaginal secretions, and semen. The aim of this study was to develop a PCR assay for the amplification of an 856-bp fragment of the rRNA gene of the Chlamydiaceae family. The PCR assay was evaluated using organs from 15 mice experimentally infected with the S26/3 reference strain of C. abortus. The results of the rRNA PCR were compared to the results from another PCR system (Omp2 PCR) that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Ovine; Caprine; Reproductive failures; Chlamydophila abortus; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700014

Phenotypic and molecular characterization of Staphylococcus xylosus: technological potential for use in fermented sausage
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Fiorentini,Ângela Maria; Sawitzki,Maristela Cortez; Bertol,Teresinha Marisa; Brod,Fábio Cristiano Angonesi; Pelisser,Márcia Regina; Arisi,Ana Carolina Maisonnave; Sant'Anna,Ernani Sebastião.
Micrococcaceae strains are applied to fermented sausage as starter cultures, where several members of this family are naturally found. The aim of the present work was to isolate and characterize Staphylococcus xylosus from artisanal sausages produced in South Region of Brazil. From 89 isolates presenting catalase positive and coagulase negative activities, 25 strains were selected for phenotypic characterization. Nine strains identified as Staphylococcus xylosus by API-STAPH were evaluated for their nitrate reduction capacity, which showed satisfactory growth of the strains in the presence of nitrite and sodium chloride, demonstrating their potential for use as starter cultures in fermented sausage. The strains were also evaluated through genus and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus xylosus; Ermented sausage; Starter cultures; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300026

Quality Control of Biotechnological Inputs DetectingMycoplasma
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Netto,Cristiane; Soccol,Vanete Thomaz; Sepulveda,Lya; Garcia,Gabriel Henrique Oliveira; Timenetsky,Jorge.
The aim of this work was to study the Polymerase Chain Reaction (PCR) as a tool of quality control of bovine sera and cellular cultures used in the biotechnological industry. A total of 46 samples of bovine sera derived from two slaughterhouses and 33 samples of BHK21 cells derived from two biotechnological industries were evaluated using the primers GPO-3 (sense) and MGSO (antisense). The PCR technique sensibility analysis showed that 280 bp were amplified for the quantities of 50 ng to 0.006 ng of Micoplasma DNA. The primers specificity was confirmed in the test using Staphylococcus aureus, Escherichia coli, Bacillus subtilisand Candida albicans; except by the positive control, none of the samples showed amplification. The presence of Mycoplasma in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Bovine serum; BHK21 cells; Mycoplasma; Cellular culture.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200239

An Alternative and Rapid Method for the Extraction of Nucleic Acids from Ixodid Ticks by Potassium Acetate Procedure
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Rodríguez,Islay; Fraga,Jorge; Noda,Angel Alberto; Mayet,Milagro; Duarte,Yanisia; Echevarria,Eduardo; Fernández,Carmen.
Four variants of the potassium acetate procedure for DNA extraction from ixodid ticks at different stage of their life cycles were evaluated and compared with phenol-chloroform and ammonium hydroxide methods. The most rapid and most efficient variant was validated in the DNA extraction procedure from the engorged ticks collected from bovine, canine as well as from house ticks for the screening of Borrelia burgdorferi sensu lato, Anaplasma spp. and Babesia spp. The ammonium hydroxide procedure was used for non-engorged ticks. All the variants were efficient and allowed obtaining PCR-quality material according to the specific amplification of 16S rRNA gene fragment of the original tick. DNA extracted from the ticks under the study was tested by multiplex PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ticks; PCR; DNA extraction.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400542

Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Rebouças,Emanuela de Lima; Costa,José Jackson do Nascimento; Passos,Maria Juliane; Passos,José Renato de Sousa; Hurk,Robert van den; Silva,José Roberto Viana.
The aim of this review was to evaluate the importance of the real-time PCR (qRT-PCR) as a technique for mRNA expression analysis in different tissues. Real-time PCR is widely used for quantification of mRNA levels and is a fundamental tool for basic research, molecular medicine and biotechnology.Genes of references are expressed in a wide variety of tissues and cells with minimal variations in their expression levels, and thus are used to normalize data of mRNA quantification. Software programs, such as geNorm, BestKeeper and NormFinder, have been developed to perform the normalization of data, which help to choose the most stable reference gene. Several genes, such as GAPDH, β-actin, β-tubulin, PGK, UBQ, RPL-19 and 18S rRNA have been suggested as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Normalization; Reference genes; Gene expression; MRNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100019

A simplified DNA extraction method for PCR analysis of Camarotella spp.
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Vitória,Nadja Santos; Bezerra,José Luiz; Gramacho,Karina Peres.
This work aimed to optimize an efficient and simple protocol for DNA extraction of Camarotella species, an obligate plant pathogen that cause verrucosis or "lixa" on coconut tree and other palms, facilitating the molecular studies of these biotrophic microorganisms. The method proposed enabled a fast, reproducible and reliable DNA extraction from Camarotella species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Camarotella torrendiella; C. acrocomiae; Verrucosis on coconut; DNA; PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200001

Sexagem de embriões bovinos fecundados in vitro pela técnica de PCR multiplex
Provedor de dados: Brazilian Jounarl of Veterinary
Autores: LUZ,Marcelo Rezende; WATANABE,Yeda Fumie; FERRO,Jesus Aparecido; FERRO,Maria Inês T.; MAURO,Sônia Marli Singaretti de; HOSSEPIAN DE LIMA,Vera Fernanda Martins; FRANCESCHINI,Paulo Henrique.
Neste trabalho, a técnica de PCR ("polymerase chain reaction") foi utilizada para a sexagem de 92 embriões bovinos fertilizados in vitro. Os embriões originaram-se de fertilização in vitro de oócitos aspirados de ovários de fêmeas bovinas, provenientes de abatedouros comerciais. Os oócitos foram maturados, fertilizados e cultivados até o estádio de blastocisto. Os embriões foram lavados em solução de PBS, transferidos para tubos de polipropileno contendo água ultrapura, e imediatamente congelados a -196ºC. Os embriões foram descongelados sobre isopor contendo gelo picado e tratados com proteinase K. Para a reação de PCR, utilizaram-se alíquotas de 34 µl de cada tudo, onde foram acrescidos dois pares de primers, seqüência BC1.2 e seqüência satélite 1.715,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sexagem; Embrião; Bovino; PCR.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000600006

Bovine carcass sexing by PCR method
Provedor de dados: Brazilian Jounarl of Veterinary
Autores: Curi,Rogério Abdallah; Mota,Lígia Souza Lima Silveira da; Amarante,Mônica Regina Vendrame; Lopes,Catalina Romeiro.
The objective of the present study was to develop a methodology that would permit sexing bovine meat ready for commercialization. A male-specific primer sequence was used, followed by analysis of the amplified product. The method proved to be efficient for sex verification and is of practical utility in the prevention of fraud in beef sale.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Carcass; Sexing; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000300007

Survey of Mycoplasma pneumoniae in Iranian children with acute lower respiratory tract infections
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Hadi,Nahal; Kashef,Sara; Moazzen,Mohammad; Pour,Michel Shamoon; Rezaei,Nima.
OBJECTIVES: Mycoplasma pneumoniae is an atypical pathogen, which is one of the major causes of lower respiratory tract infections (LRTIs) worldwide. This study was performed to determine the role of M. pneumoniae in acute LRTIs in children, who were referred to main pediatric hospitals in Shiraz, Iran, with the diagnosis of LRTI. Polymerase chain reaction method on a throat-swab specimen was utilized to detect M. pneumoniae. RESULTS: One hundred patients with acute LRTIs were investigated in this study. There were 10 positive PCR for M. pneumoniae (10%), including 6 of 62 hospitalized patients and 4 of 38 outpatients. All patients with LRTIs due to M. pneumoniae had cough. Fever, flu like symptoms, dyspnea, pulmonary rales, wheezing, and conjunctivitis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atypical bacteria; Mycoplasma pneumoniae; PCR; Respiratory infection.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200001

The investigation of parvovirus B19 infection in patients with haematological disorders by using PCR and ELISA techniques
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Us,Tercan; Ozune,Leyla; Kasifoglu,Nilgun; Akgun,Yurdanur.
Parvovirus B19 has a marked tropism for erythroid progenitor cells. This may lead to chronic anemia in predisposed individuals. The purpose of the study was to investigate the frequency of parvovirus B19 infections in patients with diagnosis of haematological disorders. In order to determine the diagnostic use of different markers of parvovirus B19 infection, serum specimens obtained from 79 patients with haematological disorders were tested for specific antibodies and viral DNA through the use of ELISA and PCR techniques. Evidence of parvovirus B19 infection was found in 23/79 (29.1%) patients by demonstrating viral DNA and/or specific IgM antibody. B19 infection was established in 3 of 11 patients with chronic myeloid leukemia, in 3 of 11 acute myeloid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; ELISA; Haematological disorder.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300006

Rapid detection of Vancomycin-Resistant Enterococci (VRE) in rectal samples from patients admitted to intensive care units
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: d'Azevedo,Pedro Alves; Santiago,Kelly Aline de Souza; Furtado,Guilherme Henrique Campos; Xavier,Diego Batista; Pignatari,Antonio Carlos Campos; Titze-de-Almeida,Ricardo.
The reduction in time required to identify vancomycin-resistant enterococci (VRE) has gained increased importance during hospital outbreaks. In the present study, we implemented a laboratory protocol to speed up the VRE screening from rectal samples. The protocol combines a medium for selective VRE isolation (VREBAC®, Probac, São Paulo) and a multiplex PCR for detection and identification of vanA and vanB resistance genes. The screening performance was analyzed in 114 specimens collected from four intensive care units. The swabs were collected at two periods: (1) during a VRE outbreak (February 2006, n=83 patients) and (2) at the post-outbreak period, after adoption of infection control measures (June 2006, n=31 patients). Forty-one/83 VRE (49.4%) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterococcus; VRE; Vancomycin; Diagnostic; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000400010

Western blot seroindeterminate individuals for Human T-lymphotropic Virus 1/2 (HTLV-1/2) in Fortaleza (Brazil): a serological and molecular diagnostic and epidemiological approach
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Santos,Terezinha de Jesus Teixeira; Costa,Carlos Maurício de Castro; Goubau,Patrick; Vandamme,Anne-Mieke; Desmyter,Jan; Van Dooren,Sonia; Mota,Rosa M. S.; Costa,Francine Bovy de Castro; Oliveira,Ana C. S.; Gomes,Vânia Barreto A.F.; Carneiro-Proietti,Anna B.; Bruin,Veralice Meireles Sales de; Sousa,Francisca C. F. de; Oriá,Reinaldo Barreto.
How to handle Western blot (WB) seroindeterminate individuals for Human T-lymphotropic Virus 1/2 (HTLV-1/2) constitutes a challenge for blood banks and fam ilies. We made a cross-sectional study of 191 enzyme linked immunoassay (EIA) reactive individuals from the hematological center (HEMOCE) of Fortaleza (Brazil), examining their serological (WB) and molecular (PCR) diagnosis, and demographic profiles, as well as a possible association of their condition with other infectious pathologies and risk factors. Ethical institutional approval and personal consent were obtained. Out of 191 EIA reactive individuals, 118 were WB seroindeterminate and 73 were seropositive for HTLV-1/2. In the PCR analysis of 41 WB seroindeterminate individuals, 9 (22%) were positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiology; PCR; HTLV-1/2; Seroindeterminate individuals; Hepatitis; Syphilis; Blood transfusion.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000300005

Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatment
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Cavalheiro,Norma de Paula; Filgueiras,Thelma Cristina; Melo,Carlos Eduardo; Morimitsu,Suzana Rie; Araújo,Evaldo Stanislau Affonso de; Tengan,Fátima Mitiko; Barone,Antonio Alci.
Although hepatitis C is mainly hepatotropic, some studies suggest that hepatitis C virus (HCV) infects peripheral blood mononuclear cells (PBMC), using them as a reservoir, which might contribute to the development of resistance to treatment. Fifty-four hepatitis-C patients, who had been submitted to treatment, were selected. Blood samples were collected on the same day for the detection of HCV RNA in serum and PBMC by PCR, using the Amplicor HCV 2.0 assay (Roche Diagnostics). HCV genotyping was performed using the INNO-LiPA HCV kit (Versant, Bayer Diagnostics). HCV RNA was detected in both serum and PBMC in 35 (64%) patients and no RNA in 16 (29.6%). Disagreement between the serum and PBMC results was observed for three patients (5.6%), with HCV RNA being...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Hepatitis C; PBMC; Treatment.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500006

Comparative performances of serologic and molecular assays for detecting human T lymphotropic virus type 1 and type 2 (HTLV-1 and HTLV-2) in patients infected with human immunodeficiency virus type 1 (HIV-1)
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Campos,Karoline Rodrigues; Gonçalves,Maria Gisele; Costa,Nadia Aparecida; Caterino-de-Araujo,Adele.
ABSTRACT The present study evaluated several techniques currently available (commercial kits and in-house assays) for diagnosing human T lymphotropic viruses types 1 and 2 in two groups of patients enrolled at HIV/AIDS specialized care services in São Paulo: Group 1 (G1), n = 1608, 1237 male/371 female, median age 44.3 years old, majority using highly active antiretroviral therapy (HAART); G2, n = 1383, 930 male/453 female, median age of 35.6 years old, majority HAART naïve. Enzyme immunoassays [(EIA) Murex and Gold ELISA] were employed for human T lymphotropic viruses types 1 and 2 screening; Western blotting (WB), INNO-LIA (LIA), real-time PCR pol (qPCR), and nested-PCR-RFLP (tax) were used to confirm infection. Samples were considered human T...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HTLV-1/2; HIV-1; Coinfection; Diagnosis; Immunoblotting; PCR; HAART.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300297

Detection of hepatitis B virus A1762T/G1764A mutant by amplification refractory mutation system
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Wang,Yong-Zhong; Zhu,Zhen; Zhang,Hong-Yu; Zhu,Min-Zhi; Xu,Xin; Chen,Chun-Hua; Liu,Long-Gen.
OBJECTIVE: To study the role of hepatitis B virus with A1762T/G1764A double mutation in liver cirrhosis and hepatocellular carcinoma, and create a sensitive, fast, accurate assay for detection of A1762T/G1764A double mutation.METHODS: We developed an accurate and fast real-time amplification refractory mutation system to detect A1762T/G1764A double mutation. Cloned hepatitis B virus genome was used as a control. Assay sensitivity was determined by serial dilution and mixed template experiments. Specificity was determined by cross experiments with wild and mutant hepatitis B virus. Fifty clinical samples were tested by the real-time amplification refractory mutation system and the results were compared with sequencing.RESULTS: The real-time amplification...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B virus; Basal core promoter mutations; Amplification refractory mutation system; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300261

Molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in two metropolitan areas of São Paulo State, southeast Brazil
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Cury,Gisele Gentile; Mobilon,Cristiane; Stehling,Eliana Guedes; Lancellotti,Marcelo; Ramos,Marcelo de Carvalho; Martinez,Roberto; Brocchi,Marcelo; Silveira,Wanderley Dias da.
One hundred and fifty-one methicillin-resistant z (MRSA) strains have been isolated from patients admitted in tertiary care hospitals in two metropolitan areas (Campinas City and Ribeirão Preto City) in the southeast region of Brazil and analyzed through PCR-based techniques [(PCR amplification of spa, coa, and housekeeping genes (arcC, aroE, gmk, pta, tpi, yqiL)] and further restriction fragment typing of coa and of housekeeping genes. The heterogeneity of spa gene was determined directly by agarose gel electrophoresis migration. The results obtained indicate the existence of three (A, B, C) main clusters. Since the strain distribution in these three clusters is much characteristic, it denotes the existence of three main clones. All strains isolated in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Nosocomial; Molecular characterization; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300002

Diagnosis of human papillomatosis by polymerase chain reaction in cases of divergence between results of hybrid capture and papanicolaou cytology
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Novaes,Luiz Carlos Garcez; Novaes,Maria Rita Carvalho Garbi; Simões-Barbosa,Augusto.
As various types of human papillomavirus (HPV) are involved in the pathogenesis of cervical cancer, correct diagnosis is of fundamental importance for screening programs. We evaluated the divergence of results between Papanicolaou cytology and hybrid capture by PCR detection of HPV DNA . A transversal study was conducted on 70 women attending private gynecological clinics in Brasilia, Brazil. PCRs were conducted with specific primers for general and high-risk HPV DNA. Based on the PCR results, hybrid capture was a superior diagnostic technique. When Papanicolaou was compared with the molecular biology methods, it was found that a positive Papanicolaou result does not necessarily indicate the presence of HPV. The agreement between PCR and hybrid capture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV; Diagnosis; Hybrid capture; PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000300003

Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Akçay,Emine; Çarhan,Ahmet; Hondur,Gözde; Tufan,Zeliha Koçak; Duru,Necati; Kılıç,Selçuk; Ensari,Ezgi Naz; Uğurlu,Nagihan; Çağıl,Nurullah.
Abstract  Background: Viral conjunctivitis are the most frequent infections in ophthalmology clinics. The diagnosis is usually relying on clinical findings and medical history. However, topical antibiotics are often used unnecessarily addition to symptomatic treatment because of unsure agents. We aimed to detect the Adenovirus, Coxsackievirus and Enterovirus from conjunctiva and pharyngeal samples of patients.  Methods: The conjunctiva and pharyngeal samples of the patients with conjunctivitis were taken by Virocult transport media and kept at -80 ºC up to study day. Adenovirus spp, Enterovirus 70 and Enterovirus 71, Coxsackie A24 and Coxsackie A16 were detected by real-time PCR. Samples from healthy health care workers of ophthalmology clinic were used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adenovirus; Conjunctivitis; Viral; PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400391

Molecular characterization of group A rotavirus isolates obtained from hospitalized children in Salvador, Bahia, Brazil
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Serravalle,Karina; Santos,Norma; Sardi,Silvia Inês; Silva,Sarah Peregrino Santos; Ribeiro Junior,Hugo da Costa; Mattos,Ângela Peixoto de; Campos,Gúbio Soares.
Rotavirus is a major cause of infectious diarrhea in infants and young children. The objective of this study was to characterize the genotypes of Human Rotavirus found in children hospitalized with acute diarrhea in the Pediatric Hospital Prof. Hosannah de Oliveira of the UFBA in Salvador, Bahia, Brazil, during the years of 1999, 2000 and 2002. Fecal samples were analyzed (n=358) by methods EIARA and SDS-PAGE for detection of Rotavirus. Positive samples of one or two of these methods (n=168) were submitted to RT-PCR and Multiplex-Nested PCR to determine genotypes G and P. A hundred sixty-eight (46.9%) samples were positive and 190 (53.1%) negative. Only 17 (4.7%) samples had divergent results. The distribution of genotypes G during the first year, showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; PCR; Genotype; Children.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100010

Detection of Chlamydia trachomatis in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCR
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Santos,Cristina; Teixeira,Fabiane; Vicente,Ana; Astolfi-Filho,Spartaco.
Chlamydia trachomatis is now one of the most prevalent bacteria found in classic sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. We examined the prevalence of Chlamydia trachomatis, by polymerase chain reaction (PCR), in 121 sexually active women who sought treatment for STD in the Alfredo da Matta Institute of Dermatology and Venerology and the Institute of Tropical Medicine of Amazonas in Manaus, Brazil. These women were examined by a specific PCR for the chlamydial plasmid, and the nature of the amplicon was determined by restriction analysis and DNA sequencing. The PCR diagnosis revealed a prevalence of 20.7% infected women.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlamydia trachomatis; PCR; DST.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200001

Papular-purpuric "gloves and socks" syndrome caused by parvovirus B19 infection in Brazil: a case report
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Toyoshima,Marcos Tadashi Kakitani; Keller,Lilian Walsh; Barbosa,Maria Luisa; Durigon,Edison Luiz.
Papular-purpuric "gloves and socks" syndrome (PPGSS) is a novel, rare, self-limiting dermatosis caused by human parvovirus B19. It consists of pruritic edema and erythema of the hands and feet in a gloves-and-socks distribution, and it is associated with oral lesions and fever. We present a case of PPGSS in a 22-year-old Brazilian woman. Clinical and laboratory evaluation, including serological tests, PCR and gene sequencing, confirmed the presence of human parvovirus B19.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Dermatosis; Gloves and socks; Parvovirus B19; PCR; Sequencing.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000100013

Dengue: a review of the laboratory tests a clinician must know to achieve a correct diagnosis
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: De Paula,Sérgio Oliveira; Fonseca,Benedito Antônio Lopes da.
Dengue is the most important disease caused by an arbovirus (1, 2, 3 and 4 serotypes) worldwide, especially in the tropical and sub-tropical regions. Its clinical manifestations range from asymptomatic infections to a severe disease characterized by hemorrhage and shock. The incidence of dengue virus activity in the Americas has substantially increased from 1980 to 1994. In Brazil, the increase in the incidence of dengue is especially linked to the dissemination of Aedes aegypti. Thus, a rapid and accurate dengue diagnosis is of paramount importance for effective control of dengue outbreaks [8]. Five serological tests have been used for the diagnosis of dengue infection: hemagglutination-inhibition (HI), complement fixation (CF), neutralization test (NT),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dengue; Diagnosis; Serology; PCR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000600002

BKV-infection in kidney graft dysfunction
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Montagner,Juliana; Michelon,Tatiana; Fontanelle,Barbara; Oliveira,Alexandre; Silveira,Janaina; Schroeder,Regina; Neumann,Jorge; Keitel,Elizete; Alexandre,Claudio Osmar Pereira.
INTRODUCTION: BKV nephropathy (BKN) causes kidney graft loss, whose specific diagnosis is invasive and might be predicted by the early detection of active viral infection. OBJECTIVE: Determine the BKV-infection prevalence in late kidney graft dysfunction by urinary decoy cell (DC) and viral DNA detection in urine (viruria) and blood (viremia; active infection). METHODS: Kidney recipients with &gt;1 month follow-up and creatinine &gt;1.5 mg/dL and/or recent increasing &gt;20% (n = 120) had their urine and blood tested for BKV by semi-nested PCR, DC searching, and graft biopsy. PCR-positive patients were classified as 1+, 2+, 3+. DC, viruria and viremia prevalence, sensitivity, specificity, and likelihood ratio (LR) were determined (Table 2x2). Diagnosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BK virus; Decoy cells; Kidney transplantation; PCR; Viremia; Viruria.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200010

Hepatitis C virus detection in the semen of infected patients
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Cavalheiro,Norma de Paula; Santos,Ana Carolina de Oliveira; Melo,Carlos Eduardo; Morimitsu,Suzana Rie; Barone,Antonio Alci.
Though HCV infection is a serious public health problem, some aspects of its biology are still not well understood, such as its transmission through seminal fluid and sexual transmission. We looked for HCV in the semen of infected patients. Thirteen patients were included. Semen fractions (seminal plasma, leukocytes and spermatozoa) were separated with 45% and 90% Percoll gradients. The HCV-RNA in blood and semen fractions was extracted using the same protocol (AMPLICOR Roche) and was detected using the qualitative Roche Amplicor test and by agarose gel electrophoresis, with ethidium bromide staining. The mean age of the patients was 40.7 years. Risk factors for the acquisition of HCV included injectable and inhaled drug use in six (42.8%), blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; PCR; Sexual transmission; Semen.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500003

Polymerase chain reaction as a useful and simple tool for rapid diagnosis of tuberculous meningitis in a Brazilian tertiary care hospital
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Dora,José Miguel; Geib,Guilherme; Chakr,Rafael; Paris,Fernanda de; Mombach,Alice Beatriz; Lutz,Larissa; Souza,Carolina Fischinger Moura de; Goldani,Luciano Z.
Meningitis is a severe and potentially fatal form of tuberculosis. The diagnostic workup involves detection of acid-fast bacilli (AFB) in the cerebrospinal fluid (CSF) by microscopy or culture, however, the difficulty in detecting the organism poses a challenge to diagnosis. The use of the polymerase chain reaction (PCR) in the diagnostic approach to Mycobacterium tuberculosis (MTB) meningitis has been reported as a fast and accurate method, with several commercial kits available. As an alternative, some institutions have been developing inexpensive in house assays. In our institution, we use an in house PCR for tuberculosis. We analyzed the performance of our PCR for the diagnosis of MTB meningitis in 148 consecutive patients, using MTB culture as gold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculous meningitis; Diagnosis; Polymerase chain reaction; PCR; Mycobacterium tuberculosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300015

Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry and real-time PCR in a combined protocol for diagnosis of bloodstream infections: a turnaround time approach
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Quiles,Milene Gonçalves; Boettger,Bruno Cruz; Inoue,Fernanda Matsiko; Monteiro,Jussimara; Santos,Daniel Wagner; Ponzio,Vinicius; Carlesse,Fabianne; Cappellano,Paola; Carvalhaes,Cecilia Godoy; Pignatari,Antonio Carlos Campos.
ABSTRACT Bloodstream infections (BSIs) are serious infections associated with high rates of morbidity and mortality. Every hour delay in initiation of an effective antibiotic increases mortality due to sepsis by 7%. Turnaround time (TAT) for conventional blood cultures takes 48 h, forcing physicians to streamline therapy by exposing patients to broad-spectrum antimicrobials. Our objective was (1) to evaluate the accuracy and TAT of an optimized workflow combining direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and in-house real-time polymerase chain reaction (PCR) for bacterial identification and antimicrobial resistance profiling directly from positive blood bottles for diagnosing bloodstream infections...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bloodstream infections; Molecular diagnosis; Molecular panels; PCR; Mass spectrometry.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300164

Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Michelim,Lessandra; Muller,Gabriela; Zacaria,Jucimar; Delamare,Ana Paula Longaray; Costa,Sérgio Olavo Pinto da; Echeverrigaray,Sergio.
Proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (PFGE) or ribotyping, have been developed for this pathogen. However, these methods are labor intensive and time-consuming. We evaluated the discriminatory power of several PCR-based fingerprinting methods (RAPD, ISSR, ERIC-PCR, BOX-PCR and rep-PCR) for P. mirabilis clinical isolates. Typing patterns and clustering analysis indicated that RAPD, BOX-PCR and ERIC-PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proteus mirabilis; Molecular markers; Fingerprinting; PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500014

HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Abba,M.C.; Gómez,M.A.; Golijow,C.D.
In the present study we used a simple and reliable method for HLA-DQA1 allele typing based on the single-stranded conformation polymorphism (SSCP) properties of DNA molecules obtained by PCR. The technique consists of PCR amplification of a DNA fragment comprising the second exon of the HLA-DQA1 gene, amplicon denaturation using a low ionic strength solution (LIS), and electrophoresis on a small native polyacrylamide gel, followed by a rapid silver staining procedure. In order to validate the technique and to obtain the allele patterns for the DQA1 gene, 50 cervical samples were typed using this methodology and the commercial Amplitype® HLA DQA1 Amplification and Typing kit. All the alleles detected with the kit were characterized by the LIS-SSCP approach....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HLA-DQA1; PCR; LIS-SSCP.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700005

Low frequency of p53 mutations in cervical carcinomas among Brazilian women
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pinheiro,N.A.; Villa,L.L.
Human papillomavirus (HPV) infections of the high-risk types are strongly linked to the development of cervical carcinoma. The HPV oncoproteins E6 and E7 are thought to play a crucial role in this process through their interactions with the p53 protein and the retinoblastoma susceptibility gene product pRb, respectively. E6 binds to p53 protein promoting its degradation. This is considered to contribute to the oncogenesis of HPV-associated anogenital cancer. On the other hand, in HPV-negative cervical carcinoma, p53 mutations are thought to have a role in the transformation process. A total of 122 HPV-positive cervical carcinoma tissue samples were evaluated for the presence of mutations in exons 5-8 of the p53 gene by single-stranded conformation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single-stranded conformation polymorphism; SSCP; Suppressor gene; HPV; PCR; DNA sequencing.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600005

Genetic variability of hepatitis A virus isolates in Rio de Janeiro: implications for the vaccination of school children
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Villar,L.M.; Lampe,E.; Meyer,A.; Gaspar,A.M.C.
The epidemiology of hepatitis A virus (HAV) infection is shifting from high to intermediate endemicity in Brazil, resulting in increased numbers of susceptible individuals and a greater potential for the emergence of outbreaks. Universal vaccination against HAV has been recommended for children, but updated sero-epidemiological data are necessary to analyze the level of natural immunity and to identify candidates for preventive measures. In addition, more molecular studies are necessary to characterize the genotypes involved in HAV infections and outbreaks. Sera from 299 school children (5-15 years old) and 25 school staff members, collected during an outbreak of HAV at a rural public school in June 2000, were tested for IgM and total anti-HAV antibodies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis A virus (HAV); PCR; Molecular epidemiology; Sero-epidemiology; Vaccination.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200003

Detection of point mutations by non-isotopic single strand conformation polymorphism
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pinheiro,N.A.; Moura,R.P.; Monteiro,E.; Villa,L.L.
We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53. The protocol does not require any particular facilities or equipment, such as radioactive handling, large gel units for sequencing, or a semiautomated electrophoresis system. This technique consists of amplification of DNA fragments by PCR with specific oligonucleotide primers, denaturation, and electrophoresis on small neutral polyacrylamide gels, followed by silver staining. The sensitivity of this procedure is comparable to other described techniques and the method is easy to perform and applicable to a variety of tissue specimens.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: P53 gene; Human papillomavirus; PCR; SSCP; Cancer; Silver staining.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100008

Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: López-López,P.; Martínez-López,M.C.; Boldo-León,X.M.; Hernández-Díaz,Y.; González-Castro,T.B.; Tovilla-Zárate,C.A.; Luna-Arias,J.P.
Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dispar (non-pathogenic). The target for the PCR amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivity of the test. The study involved 62 stool samples that were collected from individuals with complaints of gastrointestinal discomfort. Of the 62 samples, 10 (16.1%) were positive for E. histolytica while 52 (83.9%) were negative. No sample was positive for E....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amebiasis; Neglected diseases; Diagnostic; PCR; DGGE; Adh112 gene.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400607

A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Devalle,S.; Niel,C.
Torque teno virus (TTV) is a circular, single-stranded DNA virus that chronically infects healthy individuals of all ages worldwide. TTV has an extreme genetic heterogeneity which is reflected in its current classification into five main phylogenetic groups (1-5). Using specific PCR assays, it has been shown that many individuals are co-infected with TTV isolates belonging to different phylogenetic groups. Here, a multiplex PCR assay was developed, using five recombinant plasmids. Each plasmid carried an insert of different size issued from a TTV isolate belonging to a different group. The assay was able to simultaneously amplify DNAs of TTV isolates belonging to all five phylogenetic groups. Multiplex PCR was then tested satisfactorily on DNAs extracted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazil; Co-infections; Multiplex; PCR; Torque teno virus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600006

Comparison of the specificity of PCR and the histopathological detection of leishmania for the diagnosis of American cutaneous leishmaniasis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Medeiros,A.C.R.; Rodrigues,S.S.; Roselino,A.M.F.
More precise and rapid diagnostic methods for American cutaneous leishmaniasis (ACL) are necessary because of the growing number of cases observed in Brazil, including the northeastern region of the State of São Paulo. We applied PCR to 54 skin or mucosal biopsies from patients with a clinical and/or laboratory diagnosis of ACL using primers 13A and 13B, with positive results being obtained for 82% of the samples. When the PCR results were compared to those of histopathological leishmania detection, PCR showed superior results with 81.5% sensitivity and 95% CI of 68.0-95.1%. The Montenegro skin test (MST) was positive in 88.7% of patients. Since MST cannot be used as a diagnostic tool in endemic areas, the present results strongly suggest the use of PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Cutaneous leishmaniasis; PCR; Histopathological detection; Leishmania detection.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400002

Detection of cytomegalovirus infections by PCR in renal transplant patients
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Costa,S.C.B.; Miranda,S.R.P.; Alves,G.; Rossi,C.L.; Figueiredo,L.T.M.; Costa,F.F.
Cytomegalovirus (CMV) is the single most important infectious agent affecting recipients of organ transplants. To evaluate the incidence and the clinical importance of CMV infection in renal transplants in Brazil, 37 patients submitted to renal allograft transplants were tested periodically for the presence of cytomegalovirus DNA in urine using the polymerase chain reaction (PCR), and for the presence of IgM and IgG antibodies against CMV by enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence (IIF). The PCR-amplified products were detected by gel electrophoresis and confirmed by dot-blot hybridization with oligonucleotide probes. Thirty-two of the 37 patients (86.4%) were positive by at least one of the three methods. In six patients,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transplantation; CMV; PCR; Kidney.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800004

Association of Bartonella spp bacteremia with Chagas cardiomyopathy, endocarditis and arrythmias in patients from South America
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Corrêa,F.G.; Pontes,C.L.S.; Verzola,R.M.M.; Mateos,J.C.P.; Velho,P.E.N.F.; Schijman,A.G.; Selistre-de-Araujo,H.S.
Infection with Bartonella spp may cause cardiac arrhythmias, myocarditis and endocarditis in humans. The aim of the present study was to evaluate a possible association between Bartonella spp bacteremia and endocarditis, arrhythmia and Chagas cardiomyopathy in patients from Brazil and Argentina. We screened for the presence of bacterial 16S rRNA in human blood by PCR using oligonucleotides to amplify a 185-bp bacterial DNA fragment. Blood samples were taken from four groups of subjects in Brazil and Argentina: i) control patients without clinical disease, ii) patients with negative blood-culture endocarditis, iii) patients with arrhythmias, and iv) patients with chronic Chagas cardiomyopathy. PCR products were analyzed on 1.5% agarose gel to visualize the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human; PCR; Bartonella spp; Chagas disease; Endocarditis; Arrhythmia.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700012

Use of conventional PCR and smear microscopy to diagnose pulmonary tuberculosis in the Amazonian rainforest area
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Carniel,F.; Dalla Costa,E.R.; Lima-Bello,G.; Martins,C.; Scherer,L.C.; Rossetti,M.L.
The diagnostic usefulness of Ziehl-Neelsen (ZN)-stained sputum smears combined with conventional polymerase chain reaction (ZN/PCR) to amplify IS6110 region DNA extracted from ZN slides was evaluated. The objective was to verify if this association could improve tuberculosis (TB) diagnosis in patients at remote sites. The study was carried out in 89 patients with culture-confirmed pulmonary TB as defined by the Brazilian Manual for TB Treatment. The participants were recruited in a reference unit for TB treatment in Rondônia, a state in the Amazonian area in northern Brazil. ZN, PCR, and culture performed in the sputum samples from these patients were analyzed in different combinations (i.e., ZN plus PCR and ZN plus culture). The prevalence rates of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Diagnosis; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201016

Use of the polymerase chain reaction to detect Mycobacterium leprae in urine
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Caleffi,K.R.; Hirata,R.D.C.; Hirata,M.H.; Caleffi,E.R.; Siqueira,V.L.D.; Cardoso,R.F.
Leprosy is an infectious disease caused by Mycobacterium leprae. The polymerase chain reaction (PCR) has been applied to detect M. leprae in different clinical samples and urine seems to be attractive for this purpose. PCR was used to improve the sensitivity for diagnosing leprosy by amplifying a 151-bp PCR fragment of the M. leprae pra gene (PCR-Pra) in urine samples. Seventy-three leprosy patients (39 males and 34 females, 14 to 78 years old) were selected for leprosy diagnosis at a reference laboratory in Maringá, PR, Brazil. Of these, 36 were under anti-leprosy multidrug therapy with dapsone and rifampicin for tuberculoid (TT) and dapsone, rifampicin and clofazimine for borderline (BB) and lepromatous (LL) forms. The control group contained 50 healthy...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: PCR; Leprosy; Mycobacterium leprae; Urine; TT leprosy; LL leprosy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200010

McFarland nephelometer as a simple method to estimate the sensitivity of the polymerase chain reaction using Mycobacterium tuberculosis as a research tool
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bollela,V.R.; Sato,D.N.; Fonseca,B.A.L.
Polymerase chain reaction (PCR) has been widely investigated for the diagnosis of tuberculosis. However, before this technique is applied on clinical samples, it needs to be well standardized. We describe the use of McFarland nephelometer, a very simple approach to determine microorganism concentration in solution, for PCR standardization and DNA quantitation, using Mycobacterium tuberculosis as a model. Tuberculosis is an extremely important disease for the public health system in developing countries and, with the advent of AIDS, it has also become an important public health problem in developed countries. Using Mycobacterium tuberculosis as a research model, we were able to detect 3 M. tuberculosis genomes using the McFarland nephelometer to assess...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tuberculosis; PCR.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900003

The use of polymerase chain reaction for early diagnosis of tuberculosis in Mycobacterium tuberculosis culture
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Chagas,M.; Silva,R.M. da; Bazzo,M.L.; Santos,J.I. dos.
Early diagnosis plays a vital role in controlling tuberculosis. The conventional methodology is slow, with results taking several weeks, in addition to having low sensitivity, especially in clinical paucibacillary samples. The objective of this study was to evaluate the use of polymerase chain reaction (PCR) on solid medium culture for a rapid diagnosis of tuberculosis, mainly in cases of negative sputum smears. Forty sputum samples were collected from inpatients with tuberculosis treated for less than 2 days. Bacilloscopy, PCR for sputum, culture on Löwestein-Jensen (LJ) solid medium, and daily PCR from culture were performed on each sample. DNA extracted from the BCG vaccine, which contains attenuated bacillus Calmette-Guérin, was used as the positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Mycobacteria; PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600004

The use of singleplex and nested PCR to detect Batrachochytrium dendrobatidis in free-living frogs
Provedor de dados: Brazilian Journal of Microbiology
Autores: Coutinho,Selene Dall'Acqua; Burke,Julieta Catarina; Paula,Catia Dejuste de; Rodrigues,Miguel Trefaut; Catão-Dias,José Luiz.
Many microorganisms are able to cause diseases in amphibians, and in the past few years one of the most reported has been Batrachochytrium dendrobatidis. This fungus was first reported in Brazil in 2005; following this, other reports were made in specimens deposited in museum collections, captive and free-living frogs. The aim of this study was to compare singleplex and nested-PCR techniques to detect B. dendrobatidis in free-living and apparently healthy adult frogs from the Brazilian Atlantic Forest. The sample collection area was a protected government park, with no general entrance permitted and no management of the animals there. Swabs were taken from the skin of 107 animals without macroscopic lesions and they were maintained in ethanol p.a. Fungal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chytridiomycosis; Batrachochytrium dendrobatidis; Frogs; Brazilian Atlantic Forest; PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200551

Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Rulík,Martin; Chaudhary,Prem Prashant.
Magnetotactic bacteria (MTB) are of considerable interest because of their importance in the manufacture of various bioinspired materials. In order to find out the status of magnetotactic bacteria at three different sediment in Czech Republic, samples collected from both standing and running freshwaters were subjected to molecular diversity analysis by using 16S rRNA gene approach. Total community DNA from sediment sample was isolated and used for PCR, cloning and sequence analysis. Of the 24 analyzed sequences, six clones are closely related to Magnetobacterium sp. affiliated with the Nitrospira phylum which showed the dominance of Magnetobacterium phylotypes in the sample. This study will provide useful insight about the community structure of MTB in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnetotactic bacteria; Sediment; PCR; Cloning; Phylogeny; 16S rRNA gene.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400016

Occurrence of methicillin resistant Staphylococcus aureus (MRSA) among clinical samples in tehran-iran and its correlation with polymorphism of specific accessory gene regulator (AGR) groups
Provedor de dados: Brazilian Journal of Microbiology
Autores: Azimian,Amir; Najar-pirayeh,Shahin; Mirab-Samiee,Siamak; Naderi,Mahmood.
Methicillin-Resistant Staphylococcus aureus (MRSA) is responsible for an increasing number of serious hospital and community acquired infections. Virulence gene expression in Staphylococcus aureus is orchestrated by regulators such as the accessory gene regulator (agr). Staphylococcal strains are divided into four major agr groups (agrI-IV) on the basis of agrD and agrC polymorphisms. The purpose of this study was to define the prevalence of MRSA strains in appointed Tehran's hospitals and then to define and compare the proportion of agr I, II, III, IV polymorphisms between MRSA and Methicillin Sensitive Staphylococcus aureus (MSSA) strains. A total of 235 isolates were evaluated by conventional antibiotic susceptibility tests and PCR for agr and mecA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methicillin Resistant Staphylococcus aureus; Agr; PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200043

Detection of Brucella abortus DNA in illegal cheese from São Paulo and Minas Gerais and differentiation of B19 vaccinal strain by means of the polymerase chain reaction (PCR)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Miyashiro,Simone; Scarcelli,Eliana; Piatti,Rosa M.; Campos,Fabíola R.; Vialta,Airton; Keid,Lara Borges; Dias,Ricardo Augusto; Genovez,Margareth E.
A total of 192 samples of illegal cheese from different regions of the states of São Paulo and Minas Gerais, Brazil, were analyzed for the isolation and detection of Brucella spp. DNA by means of microbiological culture and polymerase chain reaction (PCR), respectively. Samples that yielded positive results were submitted to the analysis of the occurrence of Brucella abortus (biovars 1, 2 e 4), as well as to the differentiation of DNA in B19 vaccinal strain or Brucella abortus field strain using PCR. Although the microorganism was not isolated from any sample, PCR detected 37 positive samples (19.27%) using genus-specific primers. From these, all (100%) were Brucella abortus. Differentiation of the strain showed that 30/37 samples (81.08%) were vaccinal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucella abortus; B19; PCR; Cheese.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100005

Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates
Provedor de dados: Brazilian Journal of Microbiology
Autores: Oliveira,Caio Fernando de; Salla,Adenilde; Lara,Valéria Maria; Rieger,Alexandre; Horta,Jorge André; Alves,Sydney Hartz.
The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production by clinical strains of Escherichia coli and Klebsiella sp. isolated from pus, urine and blood of patients at Hospital Universitário Santa Maria, Rio Grande Sul, RS, Brazil and the genotyping of the isolates based on bla SHV genes. The ESBL study was carried out using the Combined Disc Method, while Polymerase Chain Reaction (PCR) was used to study the bla SHV genes. Of the 90 tested isolates, 55 (61.1%) were identified as ESBL-producing by the combined disk...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ESBL; Enterobacteriaceae; Prevalence; PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200002

First identification of Mycobacterium avium paratuberculosis sheep strain in Argentina
Provedor de dados: Brazilian Journal of Microbiology
Autores: Travería,G.E.; Zumarraga,M.; Etchechoury,I.; Romano,M.I.; Cataldi,A.; Alvarado Pinedo,M.F.; Pavlik,I.; Pribylova,R.; Romero,J.R.
We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina. IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle (C) strains by using IS1311 PCR-restriction endonuclease analysis (PCR-REA), multiplex PCR and restriction fragment length polymorphism (RFLP) analysis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paratuberculosis; Sheep; Typing; PCR; PCR-REA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300037

Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR
Provedor de dados: Brazilian Journal of Microbiology
Autores: Gilardoni,Liliana Rosa; Fernández,Bárbara; Morsella,Claudia; Mendez,Laura; Jar,Ana María; Paolicchi,Fernando Alberto; Mundo,Silvia Leonor.
Abstract The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic separation and IS900 -PCR detection (IMS-IS1 PCR) was employed on milk samples from 265 lactating Holstein cows from Map infected and uninfected herds in Argentina. IMS-IS1 PCR results were analyzed and compared with those obtained from milk and fecal culture and serum ELISA. The extent of agreement between both tests was determined by the Kappa test. IMS-IS1 PCR showed a detection limit of 101 CFU of Map/mL of milk, when 50:50 mix of monoclonal and polyclonal antibodies were used to coat magnetic beads. All of the 118 samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium avium subsp. paratuberculosis; Immunomagnetic separation; PCR; Cow's milk.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200506

Performance of transport and selective media for swine Bordetella bronchiseptica recovery and it comparison to polymerase chain reaction detection
Provedor de dados: Brazilian Journal of Microbiology
Autores: Coutinho,Tania Alen; Bernardi,Mari Lourdes; Cardoso,Marisa Ribeiro de Itapema; Borowski,Sandra Maria; Moreno,Andrea Micke; Barcellos,David Emilio Santos Neves de.
Three comparative assays were performed seeking to improve the sensitivity of the diagnosis of Bordetella bronchiseptica infection analyzing swine nasal swabs. An initial assay compared the recovery of B. bronchiseptica from swabs simultaneously inoculated with B. bronchiseptica and some interfering bacteria, immersed into three transport formulations (Amies with charcoal, trypticase soy broth and phosphate buffer according to Soerensen supplemented with 5% of bovine fetal serum) and submitted to different temperatures (10ºC and 27ºC) and periods of incubation (24, 72 and 120 hours). A subsequent assay compared three selective media (MacConkey agar, modified selective medium G20G and a ceftiofur medium) for their recovery capabilities from clinical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bordetella bronchiseptica; Swine; Transport media; Selective media; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300009

Do DNA extraction methods and Taq polimerase quality improve the double repetitive element (DRE) PCR typing method for Mycobacterium tuberculosis strains?
Provedor de dados: Brazilian Journal of Microbiology
Autores: Cavalcanti,Hebe Rodrigues; Marques,Elizabeth; Fonseca,Leila de Souza; Saad,Maria Helena Féres.
Double repetitive element (DRE) PCR amplification is a simple Mycobacterium tuberculosis typing method, however amplification failure or poor resolution of bands commit its efficacy. In order to verify if whether or not these features could be minimized by improving DNA extraction procedures or Taq polymerise quality, DRE-PCR was performed on 24 M. tuberculosis DNA samples extracted by heat-shock, mechanical and enzymatic methods applying conventional and hot start Taq pol. We demonstrated that when dealing with the Mycobacterium tuberculosis DRE-PCR typing method, Taq pol of better quality might be more important to improve amplification than the DNA extraction method.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; PCR; Taq hot start; DRE-PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300004

Identification of Clostridium chauvoei in clinical samples cultures from blackleg cases by means of PCR
Provedor de dados: Brazilian Journal of Microbiology
Autores: Miyashiro,S.; Nassar,A.F.C.; Souza,M.C.A.M.; Carvalho,J.B.; Adegas,J.E.B.
C. chauvoei presence was detected by means of polymerase chain reaction (PCR) from supernatant of culture in cooked meat medium of liver, muscle and metatarsian bone marrow samples of seven calves with blackleg symptoms. The isolation under anaerobic conditions of one muscle sample revealed Clostridium perfringens in pure culture.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium chauvoei; Blackleg; Bovine; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300020

Comparison of two slide agglutination serotyping methods and PCR-based capsule typing for the characterization of Haemophilus influenzae serotypes
Provedor de dados: Brazilian Journal of Microbiology
Autores: Silva,Maria E.N. Bonifácio da; Silva,Paulo da; Medeiros,Marta I.C.; Neme,Suzel N.; Macedo,Claudia; Marin,José Moacir.
Ninety-three nasopharyngeal Haemophilus influenzae isolates were serotyped by two slide agglutination methods (SAST 1 and SAST 2) and the results compared with those obtained by capsule type-specific PCR. SAST 1 presented a low correlation with results obtained by PCR (75.2%) while SAST 2 showed a better agreement with the molecular technique results (93.5%). These findings suggest that SAST 2 could be an alternative method for adequate detection of H.influenzae type b.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Haemophilus influenzae; Serotyping methods; PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100007

Leptospira spp detection by Polymerase Chain Reaction (PCR) in clinical samples of captive black-capped Capuchin monkey (Cebus apella)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Scarcelli,Eliana; Piatti,Rosa Maria; Fedullo,José Daniel Luzes; Simon,Faiçal; Cardoso,Maristela Vasconcellos; Castro,Vanessa; Miyashiro,Simone; Genovez,Margareth Élide.
Leptospirosis is a widely distributed zoonosis that affects domestic and wild animals, and that has the man as the end point of its epidemiological chain. Leptospirosis diagnosis in primates is more difficult than in other animal species, as clinical signs and lesions are less evident and antibody response is detected only for short periods. The aim of this article was to describe the detection of Leptospira spp using polymerase chain reaction (PCR), in clinical samples from one captive black-capped Capuchin monkey (Cebus apella), which presented characteristics compatible with leptospirosis (jaundice and haemorrhagic kdney) in the macroscopic post-mortem examination. A friable kidney fragment and urine sample were cultured and submitted to experimental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Nonhuman primate; PCR; Cebus apella.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200010

Serological and molecular diversity of human rotavirus in São Paulo, Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Munford,Veridiana; Souza,Eloisa Correia; Caruzo,Thabata Alessandra Ramos; Martinez,Marina Baquerizo; Rácz,Maria Lúcia.
From a total of 187 fecal samples from children with ages between 0 and 5 years, collected in the Hospital Universitário -USP, Brazil, from 1994 to 1996, 54 (28.9%) were positive for rotavirus. Positive samples were characterized by electropherotyping, subgrouping, G serotype and genotype and P genotype. Rotavirus electropherotypes were characterized in four different long genome patterns (38.9%), one short genome pattern (34.0%) and 18.0% were characterized as an unusual pattern. Subgroup I was found in 38.9% strains, subgroup II in 50.0% and 7.7% was subgroup nonI-nonII. For G serotypes, G2 was found in 59.3%, G1 was identified in 33.3% of strains, two samples showed mixtures of G1+G2 and one sample was G1+G3. Ten samples characterized as serotype G2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Gastroenteritis; PCR; Serotypes; Genotypes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300014

Sensitivity, specificity and comparison of three commercially available immunological tests in the diagnosis of Cryptosporidium species in animals
Provedor de dados: Brazilian Journal of Microbiology
Autores: Danišová,Olga; Halánová,Monika; Valenčáková,Alexandra; Luptáková,Lenka.
ABSTRACT The study was conducted to compare the specificity of immunological diagnostic methods used for the diagnosis of Cryptosporidium species capable of causing life-threatening infection in both immunosuppressed and immunocompetent patients. For the detection of Cryptosporidium species in 79 animals with diarrhoea, we used three Copro-antigen tests: RIDASCREEN® Cryptosporidium test, Cryptosporidium 2nd Generation (ELISA) and RIDA®QUICK Cryptosporidium. For immunoassays we used positive and negative samples detected by means of polymerase chain reaction and validated by sequencing and nested polymerase chain reaction to confirm the presence six different species of Cryptosporidium species. Prevalence of cryptosporidiosis in the entire group determined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptosporidium spp.; Immunology tests; Comparison; PCR; Genetic diversity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100177

PCR and bioassays screening of Bacillus thuringiensis isolates from rice-fields of Rio Grande do Sul, specific to lepidopterans and coleopterans
Provedor de dados: Brazilian Journal of Microbiology
Autores: Pinto,Laura Massochin Nunes; Fiuza,Lidia Mariana.
Bacillus thuringiensis (Bt) isolates from soil samples of rice-fields in Rio Grande do Sul (RS) were tested through PCR, aiming at the screening of six groups of Bt cry genes, which codify active proteins for coleopterans and lepidopterans rice pests, and their bioinsecticide potential regarding their use in IPM system, as well. Forty six Bt isolates were grown in Agar Nutrient for 12 h and submitted to total DNA extraction. The amplified fragments were analyzed in agarose gel (1-1.5%). The screening isolates showed that 56.51% were potentially lepidopterans specific (cry1, cry2 and cry9) and 21.73% were coleopteran specific (cry3 and cry7/8), with a homogeneous distribution in the rice-field areas in Rio Grande do Sul. Cry2 genes were found just once and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus thuringiensis; PCR; Bioassay; Lepidoptera; Coleoptera.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400003

Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages
Provedor de dados: Brazilian Journal of Microbiology
Autores: Sawitzki,Maristela Cortez; Fiorentini,Ângela Maria; Brod,Fábio Cristiano Angonesi; Tagliari,Caroline; Bertol,Teresinha Marisa; Arisi,Ana Carolina Maisonnave; Sant'Anna,Ernani Sebastião.
The purpose of the present work was to characterize promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented artisanal sausage manufactured in the northwestern region of Rio Grande do Sul state, Brazil. From 127 isolates of homofermentative, Gram-positive and catalase-negative lactic acid bacteria, ten isolates were randomly selected and the phenotypic characterization and species-specific PCR were performed. Genomic DNA from each isolated strain and from the reference strains L. plantarum ATCC 8014 and L. pentosus ATCC 8041 were amplified using two pairs of L. plantarum species-specific primers (16/Lpl and LbP11/LbP12). The results of the phenotypic characterization and species-specific PCR indicated that five out of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fermented sausage; L. plantarum; Phenotypic characterization; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300031

PCR detection of four periodontopathogens from subgingival clinical samples
Provedor de dados: Brazilian Journal of Microbiology
Autores: Avila-Campos,Mario Julio.
In this study, A. actinomycetemcomitans, B. forsythus, P. gingivalis and F. nucleatum were identified from subgingival plaque from 50 periodontal patients and 50 healthy subjects. Subgingival clinical samples were collected with sterilized paper points and transported in VMGA III. From all the diluted clinical samples (1:10), DNA was obtained by boiling, and after centrifugation the supernatant was used as template. Specific primers for each bacterial species were used in PCR. PCR amplification was sensitive to identify these organisms. PCR products from each species showed a single band and can be used to identify periodontal organisms from clinical specimens. PCR detection odds ratio values for A. actinomycetemcomitans and B. forsythus were significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Putative periodontal pathogens; PCR; Periodontal disease.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100017

Detection of oral streptococci in dental biofilm from caries-active and caries-free children
Provedor de dados: Brazilian Journal of Microbiology
Autores: Silva,Andréa Cristina Barbosa da; Cruz,Jader dos Santos; Sampaio,Fábio Correia; Araújo,Demetrius Antônio Machado de.
This work correlated the presence of oral streptococci in dental biofilm with clinical indexes of caries and oral hygiene in caries-active and caries-free children. S. mutans and/or S. sobrinus in the dental biofilm does not indicate a direct risk for developing dental caries.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dental caries; Dental biofilm; Oral streptococci; PCR; Glucosyltranferases.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400009

Detection of Ehrlichia canis in domestic cats in the central-western region of Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Braga,Ísis Assis; Santos,Luana Gabriela Ferreira dos; Ramos,Dirceu Guilherme de Souza; Melo,Andréia Lima Tomé; Mestre,Gustavo Leandro da Cruz; Aguiar,Daniel Moura de.
Ehrlichiosis is a worldwide distributed disease caused by different bacteria of the Ehrlichia genus that are transmitted by arthropod vectors. Its occurrence in dogs is considered endemic in several regions of Brazil. Regarding cats, however, few studies have been done and, consequently, there is not enough data available. In order to detect Ehrlichia spp. in cats from the central-western region of Brazil, blood and serum samples were collected from a regional population of 212 individuals originated from the cities of Cuiabá and Várzea Grande. These animals were tested by the Immunofluorescence Assay (IFA) and the Polymerase Chain Reaction (PCR) designed to amplify a 409 bp fragment of the dsb gene. The results obtained show that 88 (41.5%) cats were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ehrlichiosis; Feline; PCR; IFA; Ticks.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200036

Detection of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Alves,Clebert José; Figueiredo,Salomão Moreira de; Azevedo,Sérgio Santos de; Clementino,Inácio José; Keid,Lara Borges; Vasconcellos,Sílvio Arruda; Batista,Carolina de Sousa Américo; Rocha,Vivianne Cambuí Mesquita; Higino,Severino Silvano.
To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucella ovis; Isolation; PCR; Ovine; Paraíba State.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200016

Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Rosário,Tatiana Reis do; Dib,Cristina Corsi; Roxo,Eliana; Pinheiro,Sônia Regina; Vasconcellos,Silvio Arruda; Benites,Nilson Roberti.
The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Mycobacterium bovis; Thin layer; PCR; Cattle and buffalo organs.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100031

Diagnosis of neonatal group B Streptococcus sepsis by nested-PCR of residual urine samples
Provedor de dados: Brazilian Journal of Microbiology
Autores: Cezarino,Bruno Nicolino; Yamamoto,Lidia; Del Negro,Gilda Maria Barbaro; Rocha,Daisy; Okay,Thelma Suely.
Group B streptococcus (GBS) remains the most common cause of early-onset sepsis in newborns. Laboratory gold-standard, broth culture methods are highly specific, but lack sensitivity. The aim of this study was to validate a nested-PCR and to determine whether residue volumes of urine samples obtained by non invasive, non sterile methods could be used to confirm neonatal GBS sepsis. The nested-PCR was performed with primers of the major GBS surface antigen. Unavailability of biological samples to perform life supporting exams, as well as others to elucidate the etiology of infections is a frequent problem concerning newborn patients. Nevertheless, we decided to include cases according to strict criteria: newborns had to present with signs and symptoms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GBS; Neonatal sepsis; Early-onset neonatal sepsis; Neonatal infection; PCR; Nested-PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100005

Gastric Helicobacter spp. infection in captive neotropical Brazilian feline
Provedor de dados: Brazilian Journal of Microbiology
Autores: Camargo,Pedro Luiz de; Uenaka,Simone Akemi; Motta,Maitê Bette; Adania,Cristina Harumi; Yamasaki,Letícia; Alfieri,Amauri A; Bracarense,Ana Paula F. R. L.
Ten captive neotropical Brazilian feline were submitted to gastroscopic examination and samples of gastric mucosa from fundus, corpus and pyloric antrum were evaluated for the presence of Helicobacter species. Warthin-Starry (WS) staining and PCR assay with species-specific primers and enzymatic cleavage were applied for bacterial detection and identification. Histological lesions were evaluated by haematoxylin and eosin staining. All animals showed normal gross aspect of gastric mucosa. Helicobacter heilmannii was confirmed in 100% of the samples by WS and PCR assay. Mild lymphocytic infiltrate in the lamina propria was observed in eight animals, mainly in the fundus region. Small lymphoid follicles were seen in three animals. No significant association...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter; Feline; Gastric diseases; PCR; Leopardus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100037

In vitro detection of pathogenic Listeria monocytogenes from food sources by conventional, molecular and cell culture method
Provedor de dados: Brazilian Journal of Microbiology
Autores: Khan,J.A.; Rathore,R.S.; Khan,S.; Ahmad,I.
Among current in vitro methods for identification of pathogenic Listeria monocytogenes (L. monocytogenes) rely on growth in culture media, followed by isolation, and biochemical and serological identification. Now PCR (Polymerase Chain Reaction) has been used for the rapid, sensitive and specific detection of pathogenic L. monocytogenes. The pathogenicity of the organism is highly correlated with haemolytic factor known as listeriolysin O (LLO). A total of 400 samples from meat and 250 samples from raw milk and their products were collected from various local dairy farms, dairy units and butcheries in Bareilly, India. Pure isolates of L. monocytogenes obtained after enrichment in Buffered Listeria enrichment broth (BLEB) followed by plating onto Listeria...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L. monocytogenes; Haemolysis; PCR; Vero cytotoxicity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300013

Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome
Provedor de dados: Brazilian Journal of Microbiology
Autores: Ozbey,Gokben; Aygun,Cem.
There is not much information available regarding the prevalence of the genotypes of Helicobacter pylori isolates in Turkey, particularly in eastern Turkey. The aims of this study were to detect the prevalence of different genotypes of H. pylori in Turkish patients with gastrointestinal complaints and to determine the relationship of these genotypes with clinical outcome and sex. One hundred forty H. pylori isolates were examined for the presence of its genotypes by the PCR. We found that the prevalence of vacA s1, vacA s2, cagA, cagE, iceA1, iceA2 and babA2 genes were 88.6%, 11.4%, 71.4%, 35.7%, 41.4%, 58.6%, and 62.1%, respectively. The most predominant vacA subtype was s1a (81.4%). The most vacA allelic combination detected were vacA s1m1 (65.2%)and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Prevalence; Patient; Genotypes; PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400014

16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
Provedor de dados: Brazilian Journal of Microbiology
Autores: Navarro-Noya,Yendi; Hernández-Rodríguez,César; Zenteno,Juan C; Buentello-Volante,Beatriz; Cancino-Díaz,Mario E; Jan-Roblero,Janet; Cancino-Díaz,Juan C.
Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophthalmitis; Staphylococcus epidermidis; PCR; DGGE.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100033

Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques
Provedor de dados: Brazilian Journal of Microbiology
Autores: Vieira-da-Motta,Olney; Folly,Márcio Manhães; Sakyiama,Cássia Camargo Hagen.
Contamination of fresh milk with Staphylococcus aureus was assessed comparatively through routine phenotypic (coagulase tube test and coagulase slide test) and genotypic (PCR) screening of 128 S. aureus strains isolated from 555 milk samples. These samples were collected from 362 cows with subclinical mastitis, hosted in different dairy herds at various locations of the Northern and Northeastern rural areas of the State of Rio de Janeiro, 39.7% of which were CMT-positive. All S. aureus isolates tested positive for the presence of the coagulase gene by PCR and the isolates could be grouped into four distinct classes according to the size of the PCR product. The strains also yielded variable results when assayed with coagulase test. Taken together, these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine mastitis; Staphylococcus aureus; Coagulase; PCR.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100007

Comparison of histological and molecular diagnosis of Helicobacter pylori in benign lesions and gastric adenocarcinoma
Provedor de dados: Brazilian Journal of Microbiology
Autores: César,Ana Cristina Gobbo; Cury,Patrícia Maluf; Payão,Spencer Luiz Marques; Liberatore,Paula Rahal; Silva,Ana Elizabete.
Helicobacter pylori colonization is associated with chronic gastritis, peptic ulcers, intestinal metaplasia, adenocarcinoma and lymphoma of the stomach. The objective of this study was to compare the results of the routinely used histology with molecular diagnosis for the detection of H. pylori. Eighty samples from gastric lesions (chronic gastritis, atrophic gastritis, gastric ulcer, and intestinal metaplasia), 18 gastric adenocarcinoma and 10 normal mucosa H. pylori-negative (control) samples were obtained. All samples were examined histologically (hematoxylin-eosin and Giemsa staining), and PCR amplifications of the species-specific antigen gene (H3H4) and urease A gene segment (H5H6) of H. pylori were made, using the human gene CYP1A1 for DNA quality...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Gastric lesions; Adenocarcinoma; Histological diagnosis; PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100003

Bacterial ecology of abattoir wastewater treated by an anaerobic digestor
Provedor de dados: Brazilian Journal of Microbiology
Autores: Jabari,Linda; Gannoun,Hana; Khelifi,Eltaief; Cayol,Jean-Luc; Godon,Jean-Jacques; Hamdi,Moktar; Fardeau,Marie-Laure.
Abstract Wastewater from an anaerobic treatment plant at a slaughterhouse was analysed to determine the bacterial biodiversity present. Molecular analysis of the anaerobic sludge obtained from the treatment plant showed significant diversity, as 27 different phyla were identified. Firmicutes, Proteobacteria, Bacteroidetes, Thermotogae, Euryarchaeota (methanogens), and msbl6 (candidate division) were the dominant phyla of the anaerobic treatment plant and represented 21.7%, 18.5%, 11.5%, 9.4%, 8.9%, and 8.8% of the total bacteria identified, respectively. The dominant bacteria isolated were Clostridium, Bacteroides, Desulfobulbus, Desulfomicrobium, Desulfovibrio and Desulfotomaculum. Our results revealed the presence of new species, genera and families of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial ecology; Biodiversity; Bacterial culture; SSCP; PCR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100073

Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with aggressive periodontitis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Rosalem Junior,Wilson; Souza,Rodrigo Carvalho de; Andrade,Arnaldo Feitosa Braga de; Colombo,Ana Paula Vieira.
Actinobacillus actinomycetemcomitans (Aa) is considered a major etiologic agent of aggressive periodontitis but this species has also been associated with other forms of periodontal disease. Further, highly leukotoxic strains are related to severity of disease. The purpose of this study was to determine the prevalence of Aa and the occurrence of the leukotoxin gene 530-bp deletion in patients with generalized aggressive periodontitis (GAP) from a Brazilian population. Thirty periodontally healthy and 29 GAP subjects participated in the study. Full-mouth periodontal examination including probing pocket depth (PPD), clinical attachment level (CAL), supragingival biofilm (SB) and bleeding on probing (BOP) was carried out at 6 sites/tooth for all subjects....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Generalized aggressive periodontitis; PCR; Actinobacillus actinomycetemcomitans; Saliva; Leukotoxin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200006

Action of phosphorylated mannanoligosaccharides on immune and hematological responses and fecal consistency of dogs experimentally infected with enteropathogenic Escherichia coli strains
Provedor de dados: Brazilian Journal of Microbiology
Autores: Gouveia,E.M.M.F.; Silva,I.S.; Nakazato,G.; Onselem,V.J.V.; Corrêa,R.A.C.; Araujo,F.R.; Chang,M.R.
The therapeutic action of phosphorylated mannanoligosaccharides (MOS) was investigated regarding its prebiotic activity on enteropathogenic Escherichia coli (EPEC). Diarrhea was induced in dogs by experimental infection with EPEC strains. Then MOS was supplied once a day, in water for 20 days. Immunological (IgA and IgG), hematological (lymphocytes, neutrophils and monocytes) and bacteriological variables (PCR detection of the eae gene of EPEC recovered from stool culture), as well as occurrence of diarrhea were evaluated. All strains caused diarrhea at 24, 48 and 72 h after infection. PCR results indicated that E. coli isolated from stool culture of all infected animals had the eae gene. There was no significant difference among groups as to number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteropathogenic E. coli; In vivo experiment; Dogs; Phosphorylated 31 mannanoligo saccharides; PCR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200027

Genotypic characterization of psittacid herpesvirus isolates from Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Luppi,Marcela Miranda; Luiz,Ana Paula Moreira Franco; Coelho,Fabiana Magalhães; Ecco,Roselene; Fonseca,Flávio Guimarães da; Resende,Mauricio.
Abstract Thirty-six isolates of psittacid herpesvirus (PsHV), obtained from 12 different species of psittacids in Brazil, were genotypically characterized by restriction fragment length polymorphism (RFLP) analysis and PCR amplification. RFLP analysis with the PstI enzyme revealed four distinct restriction patterns (A1, X, W and Y), of which only A1 (corresponding to PsHV-1) had previously been described. To study PCR amplification patterns, six pairs of primers were used. Using this method, six variants were identified, of which, variants 10, 8, and 9 (in this order) were most prevalent, followed by variants 1, 4, and 5. It was not possible to correlate the PCR and RFLP patterns. Twenty-nine of the 36 isolates were shown to contain a 419 bp fragment of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psittacid herpesvirus; PstI; RFLP; PCR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100217

Active human Cytomegalovirus infection and Glycoprotein B genotypes in Brazilian pediatric renal or hematopoietic stem cell transplantation patients
Provedor de dados: Brazilian Journal of Microbiology
Autores: Dieamant,Débora de Campos; Bonon,Sandra Helena Alves; Prates,Liliane Cury; Belangelo,Vera Maria Santoro; Pontes,Erika R.; Costa,Sandra Cecília Botelho.
A prospective analysis of active Human Cytomegalovirus infection (HCMV) was conducted on 33 pediatric renal or hematopoietic stem cell post-transplant patients. The HCMV-DNA positive samples were evaluated for the prevalence of different gB subtypes and their subsequent correlation with clinical signs. The surveillance of HCMV active infection was based on the monitoring of antigenemia (AGM) and on a nested polymerase chain reaction (N-PCR) for the detection of HCMV in the patients studied. Using restriction analysis of the gB gene sequence by PCR-RFLP (Restriction Fragment Length Polymorphism), different HCMV strains could be detected and classified in at least four HCMV genotypes. Thirty-three pediatric recipients of renal or bone marrow transplantation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytomegalovirus; Pediatric transplantation; GB genotypes; PCR; Antigenemia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100010

Diagnosis of the pulmonary tuberculosis by polymerase chain reaction: a comparative study between HIV - positive and - negative individuals
Provedor de dados: Brazilian Journal of Microbiology
Autores: Silva,Rosemeri Maurici da; Machado,Tairone; Bazzo,Maria Luiza.
This study was performed to assess the efficiency of polymerase chain reaction (PCR) directly from sputum for the diagnosis of pulmonary tuberculosis by comparison between HIV-positive and HIV-negative individuals. Sputum samples were collected from hospitalized patients admitted with a clinical diagnosis of pulmonary tuberculosis, and subjected to smear microscopy, culture on LJ medium and detection of M. tuberculosis by PCR. Sensitivity, specificity, and predictive values (positive and negative) were calculated using smear and/or culture at day 42 as the gold standard, by comparing the yield in HIV-positive and HIV-negative individuals. Regardless of serostatus, the technique's yield had 62% sensitivity, 70% specificity, 79% positive predictive value,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; HIV; PCR; Sputum.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100030

Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance
Provedor de dados: Brazilian Journal of Microbiology
Autores: Moraes,Paulo Ricardo de Souza; Chimara,Erica; Telles,Maria Alice da Silva; Ueki,Suely Yoko Misuka; Cunha,Eunice Atsuko Totumi; Honer,Michael Robin; Leão,Sylvia Cardoso.
Non-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as target (PRA-hsp65). With 15 of the 32 analysed isolates, results of both methods were concordant, being 8 Mycobacterium avium, 3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum and 1 Nocardia brasiliensis. TI of 12 isolates was inconclusive. Novel PRA-hsp65 patterns were observed with 11 isolates. Medical data were evaluated for inference of clinical relevance of these isolates.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium; PCR; Identification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200013

Borrelia burgdorferi sensu lato in humans in a rural area of Paraná State, Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Gonçalves,Daniela Dib; Moura,Rodrigo Assunção; Nunes,Mônica; Carreira,Teresa; Vidotto,Odilon; Freitas,Julio Cesar; Vieira,Maria Luísa.
This study describes the detection of Borrelia garinii and Borrelia burgdorferi sensu stricto (s.s.) in Brazilian individuals using PCR and DNA sequencing. Our results suggest that these species are emerging pathogens in this country, and additional studies are necessary to determine the epidemiological characteristics of this disease in Brazil.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Brazil; Human; Lyme borreliosis; PCR; Sequencing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200571

Molecular analysis of the rRNA genes of Babesia spp and Ehrlichia canis detected in dogs from Ribeirão Preto, Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Oliveira,L.P.; Cardozo,G.P.; Santos,E.V.; Mansur,M.A.B.; Donini,I.A.N.; Zissou,V.G.; Roberto,P.G.; Marins,M.
The partial DNA sequences of the 18S rRNA gene of Babesia canis and the 16S rRNA gene of Ehrlichia canis detected in dogs from Ribeirão Preto, Brazil, were compared to sequences from other strains deposited in GenBank. The E. canis strain circulating in Ribeirão Preto is identical to other strains previously detected in the region, whereas the subspecies Babesia canis vogeli is the main Babesia strain circulating in dogs from Ribeirão Preto.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Babesia canis vogeli; Ehrlichia canis; Ticks; RRNA gene; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200006

Human papillomavirus infection in women from Tlaxcala, Mexico
Provedor de dados: Brazilian Journal of Microbiology
Autores: Velázquez-Márquez,Noé; Jiménez-Aranda,Lucio Jaime; Sánchez-Alonso,Patricia; Santos-López,Gerardo; Reyes-Leyva,Julio; Vallejo-Ruiz,Verónica.
Cervical cancer is an important health problem in women living in developing countries. Infection with some genotypes of human papillomavirus (HPV) is the most important risk factor associated with cervical cancer. Little information exists about HPV genotype distribution in rural and suburban regions of Mexico. Thus, we determined the prevalence of HPV genotypes in women from Tlaxcala, one of the poorest states in central Mexico, and we evaluated age infection prevalence and risk factors associated with cervical neoplasm. A cross-sectional study was conducted in 236 women seeking gynecological care at the Mexican Institute for Social Security in Tlaxcala, Mexico. Cervical scrapings were diagnosed as normal, low-grade, and high-grade squamous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiology; Human papillomavirus; Mexico; PCR; Squamous intraepithelial lesions.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300027

PCR and ELISA (VIDAS ECO O157®) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO
Provedor de dados: Brazilian Journal of Microbiology
Autores: Carvalho,Rosangela Nunes; Oliveira,Antonio Nonato de; Mesquita,Albenones José de; Rezende,Cíntia Silva Minafra e; Mesquita,Adriano Queiroz de; Romero,Rolando Alfredo Mazzoni.
Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157:H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157®, bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157:H7 was detected in 6.67% samples collected in open air markets using ELISA,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Minas Frescal cheese; STEC/VTEC; PCR; VIDAS ECO O157®.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100002

Identification of Staphylococcus aureus, S. intermedius and S. hyicus by PCR amplification of coa and nuc genes
Provedor de dados: Brazilian Journal of Microbiology
Autores: Silva,Wladimir Padilha da; Silva,Jorge Adolfo; Macedo,Márcia Raquel Pegoraro de; Araújo,Márcia Ribeiro de; Mata,Márcia Magalhães; Gandra,Eliezer Avila.
Sixty-five strains of coagulase positive staphylococci (Staphylococcus aureus, S. intermedius and S. hyicus) were identified at species level by PCR amplification of the coa gene, specific for S. aureus, and of the nuc gene, specific for S. intermedius and for S. hyicus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulase positive staphylococci; PCR; Coa gene; Nuc gene.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500043

Evaluation of DNA extraction protocols for Brucella abortus pcr detection in aborted fetuses or calves born from cows experimentally infected with strain 2308
Provedor de dados: Brazilian Journal of Microbiology
Autores: Matrone,M.; Keid,L.B.; Rocha,V.C.M.; Vejarano,M.P.; Ikuta,C.Y.; Rodriguez,C.A.R.; Ferreira,F.; Dias,R.A.; Ferreira Neto,J.S.
The objective of the present study was to improve the detection of B. abortus by PCR in organs of aborted fetuses from infected cows, an important mechanism to find infected herds on the eradication phase of the program. So, different DNA extraction protocols were compared, focusing the PCR detection of B. abortus in clinical samples collected from aborted fetuses or calves born from cows challenged with the 2308 B. abortus strain. Therefore, two gold standard groups were built based on classical bacteriology, formed from: 32 lungs (17 positives), 26 spleens (11 positives), 23 livers (8 positives) and 22 bronchial lymph nodes (7 positives). All samples were submitted to three DNA extraction protocols, followed by the same amplification process with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Brucellosis; Abortion; PCR; DNA extraction.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300010

Detection of Salmonella sp. from porcine origin: a comparison between a PCR method and standard microbiological techniques
Provedor de dados: Brazilian Journal of Microbiology
Autores: Castagna,Sandra Maria Ferraz; Muller,Monika; Macagnan,Marisa; Rodenbusch,Carla Rosane; Canal,Cláudio Wageck; Cardoso,Marisa.
The aim of this study was to compare a polymerase chain reaction (PCR) method combined with selective enrichment in Rappaport-Vassiliadis broth (PCR-RVB) with standard microbiological techniques (SMT) for the generic detection of Salmonella in samples of porcine origin. Two hundred sixty eight field samples consisting of 42 sets of pooled porcine mandibular lymph nodes and tonsils, 44 samples of intestinal content, 38 pork sausage meat samples and 144 samples of feed collected from swine farms were submitted to the PCR-RVB and SMT protocols. Salmonella was detected in 54 samples using the PCR-RVB assay and in 42 samples by SMT, three of the SMT Salmonella-positive samples (one each of S. Derby, S. Panama and S. Typhimurium) being Salmonella-negative by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detection; PCR; Pork; Salmonella; Swine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400013

Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Maheshwar,P.K.; Moharram,S. Ahmed; Janardhana,G.R.
The study reports the occurrence of fumonisin producing Fusarium verticillioides in 90 samples of stored paddy (Oryza sativa L.)collected from different geographical regions ofKarnataka, India. Fumonisin producing F. verticillioides was identified based on micromorphological characteristics and PCR using two sets of primers. One set of primers was F. verticillioides species specific, which selectively amplified the intergenic space region of rDNA. The other set of primers was specific to fumonisin producing F. verticillioides. Eight paddy samples were positive for F. verticillioides. Eleven isolates obtained from these samples were capable of producing fumonisin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusarium verticillioides; Fumonisin; Paddy; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100023

Bovine Herpesvirus 4 in Parana State, Brazil: case report, viral isolation, and molecular identification
Provedor de dados: Brazilian Journal of Microbiology
Autores: Kruger,Ernesto Renato; Penha,Tania Regina; Stoffelo,Daura Regina Eira; Roehe,Paulo Michel; Ribeiro,Magda Costa; Soccol,Vanete Thomaz.
Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinaesub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-4 virus; PCR; RFLP; Fingerprint.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100279

Identification of Candida spp. by phenotypic tests and PCR
Provedor de dados: Brazilian Journal of Microbiology
Autores: Marinho,Sandra Aparecida; Teixeira,Alice Becker; Santos,Otávio Silveira; Cazanova,Ricardo Flores; Ferreira,Carlos Alexandre Sanchez; Cherubini,Karen; Oliveira,Sílvia Dias de.
The correct identification of Candida species is of great importance, as it presents prognostic and therapeutical significance, allowing an early and appropriate antifungical therapy. The purpose of this study was to identify isolates of Candida spp. from oral mucosa of 38 patients with oral candidosis evaluated in 2004 by phenotypic methods and PCR, discriminating C. albicans from the other Candida species. The tests used for phenotypic analysis were germ-tube and chlamydoconidia production, culture in CHROMAgarTM Candida, carbohydrate assimilation test, growth at 45ºC and culture in Tween 80 agar. Genotypic confirmation was performed by PCR. Phenotypic tests showed that 63.2% strains formed germ-tubes, 73.7% produced chlamydoconidia, and 63.2% showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida spp; Identification; PCR; Phenotypic tests.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200004

Bovine papillomavirus type 2 in reproductive tract and gametes of slaughtered bovine females
Provedor de dados: Brazilian Journal of Microbiology
Autores: Carvalho,Claudemir de; Freitas,Antonio Carlos de; Brunner,Olga; Góes,Luiz Gustavo Bentim; Cavalcante,Andréa Yaguiu; Beçak,Willy; Santos,Rita de Cassia Stocco dos.
Papillomaviruses are described selectively infecting epithelial tissues and are associated with many forms of cancer in different species. Considering the widespread dissemination of papillomatosis in livestock, interest is being centred on possible forms of viral transmission and respective mechanisms. In the present study, we report the detection of bovine papillomavirus (BPV) DNA sequences in female reproductive tract tissues, fluids and oocytes from slaughtered bovines not afflicted by cutaneous papillomatosis. BPV-2 DNA sequences were found in ovarian and uterine tissues as well as in oocytes, cumulus cells and uterine flushings. The presence of papillomavirus sequences in reproductive organ tissues and fluids shows that viral infection in organisms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BPV-2; Papillomatosis; Gametes; PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500028

Comparison of DNA-extraction methods and Selective Enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR)
Provedor de dados: Brazilian Journal of Microbiology
Autores: Freschi,Carla Roberta; Carvalho,Luiz Fernando de Oliveira e Silva; Oliveira,Celso José Bruno de.
The aim of this study was to compare different DNA-extraction methods and selective enrichment broths for their effectiveness to detect Salmonella Typhimurium in artificially inoculated swine feces samples (100 CFU/g) by polymerase chain reaction. After enrichment in Rappaport-Vassiliadis, selenite cystine or Müller-Kauffmann tetrathionate, aliquots were used for DNA extraction by three different methods: boiling-centrifugation, phenol-chloroform and salting-out. Aliquots of extracted DNA were then used as template in PCR. The selective enrichment broths had no effect on the efficiency of PCR when boiling-centrifugation and salting-out were used. On the other hand, phenol-chloroform was superior (P&lt;0.05) when combined to Rappaport-Vassiliadis....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; DNA; Feces; PCR; Salmonella Typhimurium.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400011

Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection
Provedor de dados: Brazilian Journal of Microbiology
Autores: Souto,Renata; Silva-Boghossian,Carina M.; Colombo,Ana Paula Vieira.
P. aeruginosa and Acinetobacter spp. are important pathogens associated with late nosocomial pneumonia in hospitalized and institutionalized individuals. The oral cavity may be a major source of these respiratory pathogens, particularly in the presence of poor oral hygiene and periodontal infection. This study investigated the prevalence of P. aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with periodontal disease or health. Samples were obtained from 55 periodontally healthy (PH) and 169 chronic periodontitis (CP) patients. DNA was obtained from the samples and detection of P. aeruginosa and Acinetobacter spp. was carried out by multiplex and nested PCR. P. aeruginosa and Acinetobacter spp. were detected in 40% and 45% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Acinetobacter spp; Subgingival biofilm; Saliva; Periodontitis; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200017

Molecular differentiation between Salmonella enterica subsp enterica serovar Pullorum and Salmonella enterica subsp enterica serovar Gallinarum
Provedor de dados: Brazilian Journal of Microbiology
Autores: Ribeiro,Simone Alves Mendes; Paiva,Jaqueline Boldrin de; Zotesso,Fábio; Lemos,Manoel Victor Franco; Berchieri Júnior,Ângelo.
S. Pullorum (SP) and S. Gallinarum (SG) are very similar. They are the agents of pullorum disease and fowl typhoid, respectively, and the two diseases are responsible for economic losses in poultry production. Although SP and SG are difficult to be differentiated in routine laboratory procedures, the ability to metabolize ornithine is a biochemical test that may be used to achieve this aim. While SP is able to decarboxylate this amino acid, SG is not. However, the isolation of strains showing atypical biochemical behavior has made this differentiation difficult. One of the genes associated with the metabolization of the amino acid ornithine is called speC, and is found in both serovars. The analysis of 21 SP and 15 SG strains by means of PCR did not enable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella Pullorum; Salmonella Gallinarum; Differentiation; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100032

Application of broad-range bacterial PCR amplification and direct sequencing on the diagnosis of neonatal sepsis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Ruppenthal,Rubia Denise; Pereira,Fabiana de Souza; Cantarelli,Vlademir Vicente; Schrank,Irene Silveira.
A broad-range bacterial PCR target to conserved regions of the 23S rDNA was applied to 306 blood culture samples from 295 infants (up to one year of age) admitted to a neonatal intensive care unit. Classic blood culture results were compared to DNA sequencing analysis of the PCR amplification products. Culture results were in agreement to DNA sequencing in 90.5% (277) of 306 samples tested, including 263 PCR and culture negative samples and 29 culture and PCR positive samples. The sensitivity of the PCR method combined with sequencing was 88%, and the specificity was 96.3%, with positive and negative predictive values of 74.3 e 98.5%, respectively. The PCR-based approach directly applied to blood culture samples, correlated well with blood culture results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sepsis; Molecular diagnosis; Neonatal; PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100006

Evaluation of methods for detection and identification of Mycobacterium species in patients suspected of having pulmonary tuberculosis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Marchi,A. M.; Juttel,I. D.; Kawacubo,E. M.; Dalmarco,E. M.; Blatt,S. L.; Cordova,C. M. M.
Tuberculosis control is a priority for the Ministry of Health policies in Brazil. In the present work, the detection of Mycobacterium tuberculosis by the Polymerase Chain Reaction (PCR) was standardized, and the laboratory diagnosis of pulmonary tuberculosis was evaluated comparing baciloscopy, culture and PCR tests. The study was carried out with 117 sputum samples from different patients suspected of having pulmonary tuberculosis, for whom physicians had ordered a baciloscopy test. Baciloscopy was performed using the Ziehl-Neelsen method, and culture was performed by incubation of treated samples in Lowenstein-Jensen's medium at 37ºC for eight weeks. For PCR, DNA was amplified with a specific pair of primers to the M. tuberculosis complex, with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Baciloscopy; Culture; PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400003

Combination of commercially available molecular assays and culture based methods in diagnosis of tuberculosis and drug resistant tuberculosis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Gkaravela,Lamprini; Papadimitriou-Olivgeris,Matthaios; Foka,Antigoni; Kolonitsiou,Fevronia; Spiliopoulou,Anastasia; Charokopos,Nikolaos; Voulgaridis,Apostolos; Tsiamita,Maria; Marangos,Markos; Anastassiou,Evangelos D.; Spiliopoulou,Iris.
ABSTRACT Early diagnosis of tuberculosis is of major clinical importance. Among 4733 clinical specimens collected from 3363 patients and subjected to Ziehl-Neelsen microscopy, 4109 were inoculated onto Löwenstein-Jensen slants and 3139 in Bactec/9000MB. Polymerase Chain Reaction (PCR) was performed in 3139 specimens, whereas, a genotypic assay was directly applied in 93 Mycobacterium tuberculosis complex PCR-positive for isoniazid and rifampicin resistance detection specimens (GenoType MTBDRplus). Recovered M. tuberculosis isolates (64) as well as, 21 more sent from Regional Hospitals were tested for antimycobacterial resistance with a phenotypic (manual MGIT-SIRE) and a genotypic assay (GenoType MTBDRplus). PCR in the clinical specimens showed excellent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; PCR; GenoType MTBDRplus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400785

Experimental infection of BHK21 and Vero cell lines with different Mycoplasma spp.
Provedor de dados: Brazilian Journal of Microbiology
Autores: Netto,Cristiane; Soccol,Vanete Thomaz; Sepulveda,Lya Madureira; Timenetsky,Jorge.
Mycoplasma spp, belongs to the class Mollicutes and is capable to produce alterations in cellular cultures causing damages to the biotechnological industry. Bioproducts generally require two essential inputs, bovine serum and cells. The study herein aims to evaluate the mycoplasma concentrations that affect the growing of BHK21 and Vero cells. The species used were: Mycoplasma orale, M. salivarium, M. arginini and M. hyorhinis, cultivated in a SP4 media. Two contamination tests were performed with BHK21 and Vero cells and one of them applied different concentrations of mycoplasma. In the first one, mycoplasma was applied at the day zero and, in the second one, the contamination was performed after the monolayer establishment. The both cellular cultures...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth reduction; PCR; Biotechnological products.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400048

Improved diagnosis of central nervous system tuberculosis by MPB64-Target PCR
Provedor de dados: Brazilian Journal of Microbiology
Autores: Dil-Afroze,; Mir,Abdul Waheed; Kirmani,Altaf; Shakeel-ul-Rehman,; Eachkoti,Rafiqa; Siddiqi,Mushtaq A.
Central nervous system (CNS) tuberculosis is a serious clinical problem, the treatment of which is sometimes hampered by delayed diagnosis. Clearly, prompt laboratory diagnosis is of vital importance as the spectrum of disease is wideand abnormalities of the cerebrospinal fluid (CSF) are incredibly variable. Since delayed hypersensitivity is the underlying immune response, bacterial load is very low. The conventional bacteriological methods rarely detect Mycobacterium tuberculosis in CSF and are of limited use in diagnosis of tuberculous meningitis (TBM). This double blind study was, therefore, directed to the molecular analysis of CNS tuberculosis by an in-house-developed PCR targeted for amplification of a 240bp nucleotidesequence coding for MPB64...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CNS tuberculosis; Tuberculous meningitis (TBM); Intracranial tuberculomas; PCR; Mycobacterium tuberculosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200002

Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb
Provedor de dados: Brazilian Journal of Microbiology
Autores: Sepová,H. Kiňová; Dubničková,M; Bilková,A; Bukovský,M; Bezáková,L.
The lactic acid bacterium E isolated from the stomach mucus of breast-fed lamb was identified by sequencing of 16S rDNA fragment and species-specific PCR as Lactobacillus reuteri. Its potential antimicrobial activity and ability to modulate immune system in vitro and in vivo was determined. The growth inhibition of potential pathogens decreased from Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica ser. Minnesota to Escherichia coli. The lowest inhibition activity was observed in the case of Candida albicans. The ability of L. reuteri E to modulate biological activities of human and mouse mononuclear cells was estimated in vitro and in vivo, respectively. The production of IL-1β by monocytes in vitro was significantly induced by L. reuteri...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus reuteri; Mononuclear cells; PCR; Antagonistic activity; Phagocytosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300044

Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Miraglia,Fabiana; Moreno,Andréa Mike; Gomes,Cleise Ribeiro; Paixão,Renata; Liuson,Esequiel; Morais,Zenaide Maria; Maiorka,Paulo; Seixas,Fabiana Kömmling; Dellagostin,Odir Antonio; Vasconcellos,Silvio Arruda.
With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers &gt; 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pomona; Swine; VNTR; Culture; PCR; Genital Tract.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017

Detection of virulence genes in Salmonella Enteritidis isolated from different sources
Provedor de dados: Brazilian Journal of Microbiology
Autores: Oliveira,Sílvia Dias de; Rodenbusch,Carla Rosane; Michael,Geovana B.; Cardoso,Marisa I.R.; Canal,Cláudio Wageck; Brandelli,Adriano.
The presence of three virulence genes, invA, spvR, and spvC, was determined in Salmonella Enteritidis isolated from poultry, pigs, humans and food. All isolates were positive for the invA gene, with 91.2% being positive for spvR and 90.2% for spvC. There was no significant difference in the prevalence of the virulence genes between isolates from different sources. The results indicate that there is a putative high virulence potential for the S. Enteritidis isolates characterized.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella Enteritidis; Virulence; Spv; PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500042

Molecular detection of Brucella species in patients suspicious of Brucellosis from Zanjan, Iran
Provedor de dados: Brazilian Journal of Microbiology
Autores: Garshasbi,Maryam; Ramazani,Ali; Sorouri,Rahim; Javani,Siamak; Moradi,Soheila.
Brucella is an intracellular pathogen capable of infecting animals and humans. The aim of this study was to identify Brucella spp in sera of high risk individuals by a polymerase chain reaction (PCR)-based method. A total of 180 patients suspected to have Brucellosis were examined by serological tests. To establish a PCR protocol for diagnosis of active brucellosis, DNA was extracted from the serum samples by using a commercial kit. PCR amplification was done for detection of Brocella DNA using BCSP31 target gene and IS711 locus. The PCR assay showed that an amplicon of 223 bp was obtained in 73.8% (133/180) of the tested sera using primers (B4/B5) derived from a gene encoding the 31-kDa Brucella abortus antigen. In another PCR, an amplicon of 498 bp was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Serologic tests; Brucella abortus; Human Brucellosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200022

Molecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms
Provedor de dados: Brazilian Journal of Microbiology
Autores: Souza,Viviane; Nader Filho,Antonio; Melo,Poliana de Castro; Ferraudo,Guilherme Moraes; Ferraudo,Antônio Sérgio; Conde,Sandra de Oliveira; Fogaça Junior,Flavio Augusto.
The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples' collection sites with the highest isolation frequency of Staphylococcus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Staphylococcus aureus; Bulk tank; PCR; PFGE.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400050

Epstein-Barr virus-associated gastric carcinoma in Brazil: comparison between in situ hybridization and polymerase chain reaction detection
Provedor de dados: Brazilian Journal of Microbiology
Autores: Lima,Marcos Antonio Pereira de; Ferreira,Márcia Valéria Pitombeira; Barros,Marcos Aurélio Pessoa; Pardini,Maria Inês de Moura Campos; Ferrasi,Adriana Camargo; Rabenhorst,Silvia Helena Barem.
Epstein-Barr virus (EBV) has been associated with 10% of gastric carcinomas. The aim of this study was to determine the frequency of EBV in gastric carcinomas in Brazil assessed by in situ hybridization (ISH) and PCR, which would contribute to the characterization of the clinical and pathological aspects of EBV-associated gastric carcinomas. One hundred and ninety-two gastric carcinoma cases were collected at hospitals in two Brazilian states. Seventy-three out of 151 cases were PCR(+), while 11/160 cases were ISH(+). Nine out of eleven ISH(+) cases displayed a diffuse staining pattern and 2 out of 11 a focal pattern. Both techniques showed that the EBV(+) cases were characterized by their association with males, older patients, lower gastric region,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epstein-Barr virus; Gastric carcinoma; PCR; ISH.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100048

Comparison of culture and PCR methods in the diagnosis of bacterial meningitis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Başpınar,Emel Ödemiş; Dayan,Saim; Bekçibaşı,Muhammed; Tekin,Recep; Ayaz,Celal; Deveci,Özcan; Hoşoğlu,Salih.
Abstract Our aim in this study is to compare the standard culture method with the multiplex PCR and the Speed-Oligo® Bacterial Meningitis Test (SO-BMT) – a hybridization-based molecular test method – during the CSF examination of the patients with the pre-diagnosis of acute bacterial meningitis. For the purposes of this study, patients with acute bacterial meningitis treated at the Dicle University Medical Faculty Hospital, Infectious Diseases and Clinical Microbiology Clinic between December 2009 and April 2012 were retrospectively evaluated. The diagnosis of bacterial meningitis was made based on the clinical findings, laboratory test anomalies, CSF analysis results, and the radiological images. Growth was observed in the CSF cultures of 10 out of the 57...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial meningitis; Culture; Speed-Oligo; PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200232

Detection of Leptospira spp. in the semen and urine of bulls serologically reactive to Leptospira interrogans serovar hardjo
Provedor de dados: Brazilian Journal of Microbiology
Autores: Magajevski,Fernanda Senter; Girio,Raul Jose Silva; Mathias,Luis Antonio; Myashiro,Simone; Genovez,Margareth Élide; Scarcelli,Eliana Pinheiro.
This study evaluated PCR for the detection of leptospires in semen and urine of ten serologically reactive bulls, comparing these results with those obtained with other diagnosis techniques. Two collections of materials were done in alternate days. Semen and urine samples were separated in aliquots for: direct visualization in dark field microscopy; inoculation in hamsters (for semen only); isolation in culture media; and PCR. No hamster was positive by the microscopic agglutination test (MAT); kidney and liver fragments from the hamsters were used in an isolation attempt in culture media, with one positive isolation from the kidney of a hamster inoculated with semen of one bull, and from liver of hamsters inoculated with semen of three bulls. Isolation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Semen; Urine; PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100009

Leptospirosis diagnosis using Nested-PCR
Provedor de dados: Brazilian Journal of Microbiology
Autores: Nassi,Fernanda; Seixas,Fabiana Kömmling; Jouglard,Sandra Denize Dorneles; Simionatto,Simone; Silva,Everton Fagonde; Seyffert,Núbia; Brod,Claudiomar Soares; Dellagostin,Odir Antonio.
Leptospirosis is a worldwide sanitary problem. Its clinical signs resemble that of other diseases like Dengue and Flu, and it is difficult to distinguish between them. Currently available diagnostic methods shown low sensitivity and specificity. Efforts have been made to develop simpler, faster and more efficient diagnostic methods. The aim of this work was to evaluate and optimize a Nested-PCR method for diagnosis of leptospirosis. Primers were designed to amplify a 264 bp region within the LipL32 gene. The sensitivity and specificity of the assay was evaluated using seven saprophytic serovars and 35 pathogenic serovars. This technique showed to be very specific for pathogenic serovars, however it lacked sensitivity. In order to enhance the sensitivity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500031

Detection of shigella in lettuce by the use of a rapid molecular assay with increased sensitivity
Provedor de dados: Brazilian Journal of Microbiology
Autores: Jiménez,Kenia Barrantes; McCoy,Clyde B.; Achí,Rosario.
A Multiplex Polymerase Chain Reaction (PCR) assay to be used as an alternative to the conventional culture method in detecting Shigella and enteroinvasive Escherichia coli (EIEC) virulence genes ipaH and ial in lettuce was developed. Efficacy and rapidity of the molecular method were determined as compared to the conventional culture. Lettuce samples were inoculated with different Shigella flexneri concentrations (from 10 CFU/ml to 10(7) CFU/ml). DNA was extracted directly from lettuce after inoculation (direct-PCR) and after an enrichment step (enrichment PCR). Multiplex PCR detection limit was 10(4) CFU/ml, diagnostic sensitivity and specificity were 100% accurate. An internal amplification control (IAC) of 100 bp was used in order to avoid false...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Shigella; Rapid method; Lettuce; Food.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400018

Prevalence of leukotoxic genotypes of Actinobacillus actinomycetemcomitans in Brazilians with chronic periodontitis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Rosalem Junior,Wilson; Andrade,Arnaldo Feitosa Braga de; Colombo,Ana Paula Vieira.
Actinobacillus actinomycetemcomitans is considered a major etiologic agent of aggressive periodontitis but this species has also been associated with other forms of periodontal disease. Further, highly leukotoxic strains are related to severity of disease. This investigation determined the prevalence of A. actinomycetemcomitans and the occurrence of the leukotoxin gene 530-bp deletion in Brazilian subjects with chronic periodontitis. Twenty periodontally healthy and 20 chronic periodontitis subjects were selected. Full-mouth clinical examination was carried out at 6 sites/tooth. Subgingival biofilm samples were collected from the 3 deepest sites and 3 healthy sites from periodontitis subjects, as well as from 3 sites of individuals with periodontal health....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic periodontitis; PCR; Actinobacillus actinomycetemcomitans; Subgingival biofilm; Leukotoxin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400036

Identification of bacterial agents of enteric diseases by multiplex PCR in growing-finishing pigs
Provedor de dados: Brazilian Journal of Microbiology
Autores: Baccaro,Maria Regina; Moreno,Andrea Micke; Shinya,Luciane Tieko; Dotto,Daniela Sabatini.
In Brazil, the most common bacterial enteric diseases affecting growing and finishing pigs are porcine proliferative enteritis, porcine intestinal spirochetosis, swine dysentery, and salmonellosis. The diagnosis of these diseases by routine culture techniques is expensive, difficult, time-consuming, and even impossible, in cases of porcine proliferative enteritis. The detection of pathogens by polymerase chain reaction is a highly sensitive and specific method that can be an useful tool in veterinary diagnosis. Two multiplex PCR (M-PCR) assays were tested for simultaneous detection and identification of bacterial agents associated with porcine proliferative enteritis, porcine intestinal spirochetosis, swine dysentery, and salmonellosis in diarrheic fecal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteritis; PCR; Pig; Bacteria; Diagnosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300008

Detection of enterotoxins genes in coagulase-negative staphylococci isolated from foods
Provedor de dados: Brazilian Journal of Microbiology
Autores: Cunha,Maria de Lourdes Ribeiro de Souza da; Peresi,Eliana; Calsolari,Regina Adriana Oliveira; Araújo Júnior,João Pessoa.
Staphylococcal food poisoning is caused by ingestion of enterotoxins preformed in the food contaminated essentially through human manipulation or raw material obtained from animals. Although coagulase-positive Staphylococcus aureus is the main agent responsible for food intoxication, some researches emphasise that coagulase-negative staphylococci (CNS) are able to produce staphylococcal enterotoxins and may be a potential cause of food poisoning. In the present study CNS were isolated from foods and the toxigenic capacity of the strains determined. A total of 88 food samples were analysed and 22.7% were positive for CNS strains. Staphylococcal counts ranged from 3.0 x 10² to 1.4 x 10(6) CFU/g or mL of food examined. S. epidermidis predominated among the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulase-negative staphylococci; PCR; Enterotoxins.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100013

Mycoplasmas hyorhinis in different regions of cuba: diagnosis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Lobo,Evelyn; Poveda,Carlos; Suarez,Alejandro; Hernández,Yenney; Ramírez,Ana; Poveda,José B.
M. hyorhinis is considered one of the etiological agents of arthritis in sucking pigs, but recently as seen, some strains can produce pneumonia that could not be distinguished from the mycoplasmosis caused by M. hyopneumoniae. The study was conducted to research the presence of Mycoplasma hyorhinis (M. hyorhinis ) in different regions of the country from exudates of pig lungs with typical EP lesions. Exudates from 280 pig lungs with typical EP lesions were studied using molecular techniques such as PCR, real time PCR and amplification of the 16S-23S rRNA. It was detected that the 66% of the samples studied resulted positive to M. hyorhinis, and the presence of this species was detected in all the provinces. Amplification and studies on the intergenic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Mycoplasma; Diagnostic; Enzootic pneumonia.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200039

Comparison of phenotypic and molecular tests to identify lactic acid bacteria
Provedor de dados: Brazilian Journal of Microbiology
Autores: Moraes,Paula Mendonça; Perin,Luana Martins; Silva Júnior,Abelardo; Nero,Luís Augusto.
Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lactic acid bacteria; Identification; 16sDNA; PCR; Biolog; API.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100015

Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples
Provedor de dados: Brazilian Journal of Microbiology
Autores: Singh,Harkanwaldeep; Rathore,R.S; Singh,Satparkash; Cheema,Pawanjit Singh.
In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon in DNA extracted from E. coli, Listeria, Salmonella and Staphylococcus confirmed the specificity of the primers. Of randomly collected 143 faecal samples comprising human diarrheic stools (43), cattle diarrheic faeces (48) and poultry faecal swabs (52) only 4, 3 and 8,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter jejuni; Isolation; PCR; Spiking.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100022

Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients
Provedor de dados: Brazilian Journal of Microbiology
Autores: Silva,Rosana Bellan de Oliveira; Fusco-Almeida,Ana Marisa; Matsumoto,Marcelo Teruyuki; Baeza,Lilian Cristiane; Benaducci,Tatiane; Mendes-Giannini,Maria José Soares.
Trichosporon asahii is an opportunistic pathogen, associated with a high mortality rate in immunocompromised patients. In this study, ten isolates, recovered from oral cavity and urine of patients in Intensive Care Units (ICU) over six months, were identified by classical and molecular methods, typed by RAPD and tested in vitro for susceptibility to fluconazole, itraconazole, 5-flucytosine and amphotericin B. A total agreement between the identification of Trichosporon sp by PCR based on sequences of the Internal Transcribed Spacer Regions (ITS) and on the sequences of small-subunit (SSU) ribosomal DNA (rDNA) was found. Randomly amplified of polymorphic DNA (RAPD), with primers P6 and M13, was used to determine the genomic profiles. The dendogram analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichosporon; PCR; RAPD; Susceptibility; Antifungal drugs.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300033

Analysis of the 16S rRNA gene of Anaplasma platys detected in dogs from Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Cardozo,G.P.; Oliveira,L.P.; Zissou,V.G.; Donini,I.A.N.; Roberto,P.G.; Marins,M.
Comparison of the partial DNA sequence of the 16S rRNA gene of Anaplasma platys detected in dogs from Ribeirão Preto, Brazil, to sequences of other strains previously deposited in GenBank showed that there are at least three A. platys strains circulating in South America.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anaplasma platys; 16S rRNA gene; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300017

Polymerase chain reaction and blood culture in blood donors screened by ELISA test for Chagas' disease
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Kinoshita-Yanaga,Andréa Tieko; Bértoli,Marta; Martins,Vanessa Aparecida; Mizutani,Angélica Sayuri; Toledo,Max Jean de Ornelas; Araújo,Silvana Marques de; Gomes,Mônica Lúcia.
The objective of this study was to evaluate, through blood culture and PCR, the results of the ELISA for Chagas' disease in the screening of blood donors in the public blood-supply network of the state of Paraná, Brazil, and to map the epidemiological profile of the donors with respect to their risk of infection by Trypanosoma cruzi. The negative and positive results of the ELISA were confirmed by blood culture and PCR for 190/191 individuals (99.5%). For one individual (0.5%), the ELISA was inconclusive, blood culture and IIF were negative, and IHA and PCR positive. Three individuals (1.6%) were positive for T. cruzi on all the tests. Donors were predominantly female, and natives of Paraná, of rural origin, had observed or been informed of the presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi/detection; ELISA/use for screening; Blood donors; Blood culture; PCR; Blood bank/screened donors.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100007

Molecular Tools for Rapid and Accurate Detection of Black Truffle (Tuber melanosporum Vitt.) in Inoculated Nursery Plants and Commercial Plantations in Chile
Provedor de dados: Chilean Journal of Agricultural Research
Autores: Cordero,Cecilia; Cáceres,Pablo; González,Gloria; Quiroz,Karla; Carmen,Bravo; Ramírez,Ricardo; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando.
Truffle (Tuber melanosporum Vitt.) culture is an agroforestry sector in Chile of increasing interest due to the high prices that truffles fetch in the national market and the recent evidence that its commercial production is possible in Chilean climatic and soil conditions. In this study, the efficiency of three methods of DNA extraction from a mix of 5 g of soil and roots from both nursery and field plants of Quercus ilex L. mycorrhized with T. melanosporum were evaluated, and a simple and reproducible protocol was established. Detection of T. melanosporum was performed by the technique of cleaved amplified polymorphic sequence (CAPS) from amplicons generated with the primers ADL1 (5´-GTAACGATAAAGGCCATCTATAGG-3´) and ADL3 (5´-CGTTTTTCCTGAACTCTTCATCAC-3`),...
Tipo: Journal article
Palavras-chave: Ectomycorrhizas; Molecular markers; PCR; CAPS; Ectomicorrizas; Marcadores moleculares; PCR; CAPS.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300022

Efficient protocols for the extraction of microbial DNA from the rhizosphere of hydrophilic forests in Chile
Provedor de dados: Ciencia e Investigación Agraria, Revista Latinoamericana en Ciencias de la Agricultura
Autores: Cáceres,Pablo; Cordero,Cecilia; González,Gloria; Quiroz,Karla; Bobadilla,Juan C; Bravo,Carmen; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando.
A lysis buffer-based protocol (Protocol BA), a modified lysis buffer-based protocol (Protocol BA Mod) and a commercial extraction kit (Protocol PS Kit) (Power Soil, Mo bio Laboratories, CA USA) were each evaluated for their ability to produce high-quality DNA with yields sufficient to allow its use in biodiversity studies. Similarly, the effect of liquid nitrogen on the process of cell disruption in all of the protocols that were studied. DNA yields ranged from 12.4 ng g-1 of processed soil to 9620 ng g-1 using the modified lysis buffer and commercial extraction kit, respectively. The quality of the DNA was determined by the ability of the DNA to produce efficient and reproducible polymerase chain reaction (PCR) products, using primers for universal 16S...
Tipo: Journal article
Palavras-chave: Hydrophilic forests; 16S ribosomal RNA; PCR; Rhizosphere; Soil DNA extraction.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300018

Sarcoide equino associado ao papilomavírus bovino BR-UEL-4
Provedor de dados: Ciência Rural
Autores: Anjos,Bruno Leite dos; Silva,Mariana Sá e; Diefenbach,Aline; Brito,Marilene de Farias; Seppa,Gilberto dos Santos; Brum,Mário Celso Sperotto.
Um equino, sem raça definida, macho com três anos de idade apresentou múltiplos nódulos na pele, em diversas regiões do corpo. As lesões localizavam-se predominantemente nos lábios, nas bochechas, na região submandibular e na região inguinal direita. Os tumores caracterizavam-se como sarcoides dos tipos misto, fibroblástico, verrucoso e oculto. Histologicamente apresentaram proliferação de fibroblastos dérmicos, muitas vezes ulcerado, com ou sem hiperplasia pseudoepiteliomatosa da epiderme e formação de pequenos grupos isolados de fibroblastos neoplásicos na derme superficial. Três amostras de tecido foram submetidas à extração de DNA e amplificação por PCR com oligonucleotídeos iniciadores genéricos direcionados para uma região interna do gene L1 dos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Doenças de equinos; Patologia; Papilomavírus bovino; BR-UEL-4; Sarcoide; PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000600037

Molecular demonstration of intermittent shedding of Leptospira in cattle and sheep and its implications on control
Provedor de dados: Ciência Rural
Autores: Rocha,Bruno Ribeiro; Narduche,Lorena; Oliveira,Clara Slade; Martins,Gabriel; Lilenbaum,Walter.
ABSTRACT: For a long time, it has been stated that urine leptospiral shedding is intermittent, which was observed primarily by culturing. However, culturing presents serious limitations, mainly low sensitivity, and failure on detection of leptospires cannot be neglected. PCR presents several advantages, mainly higher sensitivity. The present study aimed to analyze the occurrence of intermittency on leptospiral shedding by PCR in naturally and experimentally infected animals. In this study two experiments were conducted, the first with 60 cows naturally infected from an endemic herd. The second one was conducted in three sheep experimentally infected, each one with a different strain of Leptospira (strains Copenhageni L1-130, Canicola LO-4 and Pomona...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cows; Intermittent; PCR; Sheep; Strain.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800453

Ancillary techniques on the evaluation of canine cutaneous mast cell tumors from Brazil
Provedor de dados: Ciência Rural
Autores: Flores,Mariana Martins; Mazaro,Renata Dalcol; Langohr,Ingeborg Maria; Roy,Alma; Strother,Keith; Fighera,Rafael Almeida.
ABSTRACT: The use of histologic classification by a 2-tier grading system only, immunohistochemistry (IHC) for KIT and Ki-67 and polymerase chain reaction (PCR) for internal tandem duplications (ITD) on exon 11 has improved the prognostication of canine cutaneous mast cell tumors (CCMTs) particularly in the United States. However, these techniques are not commonly used in most Brazilian laboratories. Likewise, no studies, to date, have investigated the occurrence of ITD in CCMTs from the country. Thus, this study tested the 2-tier grading system, the immunohistochemistry for KIT and Ki-67 and the PCR for exon 11 in a group of Brazilian CCMTs with the goal of investigating the applicability of these tests in a Brazilian laboratory. Of the 39 CCMTs, 69.2%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunohistochemistry; Ki-67; Mutations; PCR; Mast cell tumor.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001804

Infecção por Hepatozoon canis em canino doméstico na região Sul do Brasil confirmada por técnicas moleculares
Provedor de dados: Ciência Rural
Autores: Lasta,Camila Serina; Santos,Andrea Pires dos; Mello,Fabíola Peixoto da Silva; Lacerda,Luciana de Almeida; Messick,Joanne Belle; Díaz González,Félix Hilário.
Hepatozoonose canina é uma doença transmitida por carrapatos e causada pelo protozoário Hepatozoon spp. No Brasil, existem poucos relatos da infecção e dados sobre sua epidemiologia, patogenicidade, seus vetores e sua caracterização genética. No presente estudo, são utilizadas técnicas moleculares para o diagnóstico e a caracterização do parasita. Foi estudado um canino que apresentava emagrecimento progressivo, anemia regenerativa, neutropenia e hiperglobulinemia. Gamontes de Hepatozoon spp. foram visualizados no esfregaço sanguíneo. DNA foi extraído do sangue, e o diagnóstico foi confirmado pela técnica da reação em cadeia da polimerase (PCR). O produto da PCR foi purificado a partir do gel e clonado, e o fragmento de 625pb foi sequenciado. A sequência...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatozoon canis; Hemoparasita; Cão; PCR; Brasil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000700028

Reação em Cadeia da Polimerase (PCR) baseada no gene cpx para detecção de Actinobacillus pleuropneumoniae em suínos natural e experimentalmente infectados
Provedor de dados: Ciência Rural
Autores: Souza,Karina Koerich de; Klein,Catia Silene; Kich,Jalusa Deon; Coldebella,Arlei; Alberton,Geraldo Camilo.
A pleuropneumonia suína é uma das mais importantes doenças respiratórias dos suínos, estando presente em todos os países produtores. Para o controle e o monitoramento da pleuropneumonia, é necessário o desenvolvimento de métodos rápidos e acurados de diagnóstico. Com o objetivo de validar a técnica da PCR, baseada no gene cpx de Actinobacillus pleuropneumoniae, em suínos sabidamente positivos, primeiramente foi realizada inoculação experimental com amostras de A. pleuropneumoniae sorotipo 5B e coletadas amostras por meio de suabe de tonsila, biópsia de tonsila e sangue para realização da técnica de PCR, isolamento bacteriológico e teste de ELISA, respectivamente. Posteriormente, estas técnicas foram aplicadas em suínos naturalmente infectados, em três...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suíno; Actinobacillus pleuropneumoniae; PCR; Diagnóstico; Gene cpx.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000700024

Molecular detection of protozoa of the Sarcocystidae family in sheep from the State of Rio Grande do Sul, Brazil
Provedor de dados: Ciência Rural
Autores: Portella,Luiza Pires; Cadore,Gustavo Cauduro; Sangioni,Luis Antonio; Alves,Marta Elena Machado; Chemeris,Raiza; Brum,Larissa Picada; Vogel,Fernanda Silveira Flores.
ABSTRACT: Infections caused by protozoa belonging to the Sarcocystidae family have worldwide distribution and are common in ruminants, leading to considerable economic losses. This study evaluates Sarcocystis spp.,  Toxoplasma gondii and Neospora caninum infections in sheep from Southwest region of Rio Grande do Sul, Brazil. Myocardium samples of 80 sheep raised on extensive system were collected. Tissue cysts were detected by direct examination and presence of infective agents was confirmed by PCR. Macroscopic evaluation did not reveal changes, but direct microscopic examination showed cysts in 76.2% (61/80, 95% CI: 66.9 - 85.9) samples, and all cysts were morphologically similar to those caused by  Sarcocystis tenella or Sarcocystis arieticanis. PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sarcocystis spp.; Toxoplasma gondii; Neospora caninum; PCR; Diagnosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901613

Detecção de Ornithobacterium rhinotracheale (ORT) por meio da reação em cadeia da polimerase (PCR)
Provedor de dados: Ciência Rural
Autores: Canal,Cláudio Wageck; Rocha,Silvio Luís da Silveira; Leão,Joice Aparecida; Fallavena,Luiz Cezar Bello; Oliveira,Silvia Dias de; Beltrão,Nilzane.
Ornithobacterium rhinotracheale (ORT) é uma bactéria Gram negativa recentemente descrita que se encontra associada às doenças do trato respiratório em criações de aves comerciais e silvestres em vários países do mundo. No Brasil, foram detectados anticorpos em um pequeno número de frangos de corte e suas matrizes dos Estados de São Paulo e Minas Gerais. Como a bactéria é fastidiosa, a Reação em Cadeia da Polimerase (PCR) torna-se útil para sua detecção e identificação. O presente trabalho visou verificar a ocorrência da ORT no Rio Grande do Sul pela detecção do DNA da bactéria. Foram coletadas 84 amostras de suabe de traquéia de aves pertencentes a 14 lotes de diferentes empresas avícolas. O DNA foi purificado e a PCR realizada com iniciadores específicos...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Ornithobacterium rhinotracheale; Patologia aviária; PCR; Detecção; Identificação.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000200032

Mannheimia haemolytica pleuropneumonia in goats associated with shipping stress
Provedor de dados: Ciência Rural
Autores: Taunde,Paula Augusto; Argenta,Fernando Froner; Bianchi,Ronaldo Michel; Cecco,Bianca Santana de; Vielmo,Andréia; Lopes,Bruna Corrêa; Siqueira,Franciele Maboni; Andrade,Caroline Pinto de; Snel,Gustavo Geraldo Medina; Barros,Claudio Severo Lombardo de; Sonne,Luciana; Pavarini,Saulo Petinatti; Driemeier,David.
ABSTRACT:  This study described the epidemiological, clinical, pathological and bacteriological aspects of Mannheimia haemolytica pleuropneumonia in goats associated with shipping stress. Forty goats transported from the Northeast to the Southern region of Brazil died during shipment, or 2-3 days after unloading. Clinical signs included dyspnea, mucopurulent nasal discharge, and coughing. All goats were necropsied, and multiple tissues were collected for histopathological analysis and involved agent identifications. All lungs showed pulmonary consolidation, predominantly affecting the cranioventral lobes, in addition to the marked fibrinous pleuritis, pleural thickening, and pleural adhesions, that affected 90% of the goats. Histologically, there was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goat diseases; Respiratory diseases; Pathology; PCR.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000100451

Otimização da técnica da PCR para a detecção de Actinobacillus pleuropneumoniae
Provedor de dados: Ciência Rural
Autores: Souza,Karina Koerich de; Klein,Catia Silene; Kich,Jalusa Deon; Coldebella,Arlei; Alberton,Geraldo Camilo.
A utilização de métodos moleculares baseados em PCR é fundamental na detecção do Actinobacillus pleuropneumoniae, sendo capaz de identificar a infecção antes do estabelecimento da doença no rebanho. Estes métodos apresentam maior sensibilidade quando comparados com métodos tradicionais de isolamento bacteriano, mas podem sofrer influência de substâncias que reduzem a especificidade do teste e proporcionam o aparecimento de amplificações inespecíficas. No intuito de reduzir as amplificações inespecíficas, observadas quando aplicada a PCR para o gene cpx em amostras de tecido tonsilar, procedeu-se a otimização da técnica, na qual foram analisados o efeito do pré-cultivo bacteriano e as diferentes temperaturas de anelamento dos iniciadores e foi introduzido,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Gene cpx; Tonsila; Actinobacillus pleuropneumoniae; Pleuropneumonia suína.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000800023

Diferenciação específica entre Taenia saginata e Taenia solium por ensaio de PCR e duplex-PCR
Provedor de dados: Ciência Rural
Autores: Jardim,Eurione Antônio Garcia da Veiga; Linhares,Guido Fontgalland Coelho; Torres,Fernando Araripe Gonçalves; Araújo,José Luiz de Barros; Barbosa,Silvia Minharro.
Este estudo teve como objetivo a padronização de protocolos e a seleção de novos primers para a identificação espécie-específica de Taenia saginata e Taenia solium através da reação em cadeia da polimerase (PCR) e duplex-PCR. Inicialmente, foram recuperadas seqüências depositadas no GenBank (acesso n° AB020399 para T. saginata e n° AB020395 para T. solium) referentes ao gene da subunidade maior do ribossomo (LSU RNAr) de tenídeos. A partir do alinhamento das seqüências, um primer genérico denominado TBR-3 (5'-ggcttgtttgaatggtttgacg- 3') foi selecionado de região conservada e, de diferentes regiões semi-conservadas, os primers específicos TBR-4 para T. saginata (5'-cgactcatgaagataaacaaggt-3') e TBR-5 (5'-cggtcgaacagaccataaatct-3') e TBR-6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Taenia saginata; Taenia solium; Teníase; Cisticercose; PCR; Duplex-PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100025

Molecular detection of Erwinia psidii in guava plants under greenhouse and field conditions
Provedor de dados: Ciência Rural
Autores: Silva,Claudênia Ferreira da; Uesugi,Carlos Hidemi; Blum,Luiz Eduardo Bassay; Marques,Abi Soares dos Anjos; Ferreira,Marisa Álvares da Silva Velloso.
ABSTRACT:  Erwinia psidii causes bacterial blight of guava ( Psidium guajava ), an important disease of this crop in Brazil. The pathogen affects branches and twigs of guava trees, reducing yield significantly. Bacterial dissemination often occurs through contaminated but asymptomatic propagating plant material. The objectives of this research were to evaluate the use of BIO-PCR and conventional PCR to detect E. psidii in inoculated guava plants grown in a greenhouse and in symptomatic and asymptomatic trees from guava orchards. Erwinia psidii strain IBSBF 1576 was inoculated (107CFU mL-1) into young guava shoots and plant tissue was analysed at 0, 5, 10, and 15 days after inoculation. Symptoms were observed after 5 days and all inoculated shoots were PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BIO-PCR; Diagnosis; Guava bacterial blight; PCR; Psidium guajava.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901528

Detecção molecular de Brucella canis em cães do Município de Cuiabá, Estado de Mato Grosso
Provedor de dados: Ciência Rural
Autores: Silva,Carla Patricia Amarante e; Almeida,Arleana do Bom Parto Ferreira de; Godoy,Isabela de; Araújo,Ana Carolina Pires de; Aguiar,Daniel Moura de; Sousa,Valéria Régia Franco; Nakazato,Luciano; Dutra,Valéria.
Este estudo teve como objetivo investigar a ocorrência da brucelose causada por Brucella canis em cães no município de Cuiabá, MT, e os possíveis fatores de risco associados durante o período de 2007 a 2008. Foram analisadas 327 amostras baseadas numa amostragem aleatória simples, abrangendo os quatro distritos do município. A ocorrência da B. canis foi de 24,1% (I.C. 95%: 19,7 - 29,0%). A análise estatística dos sinais clínicos demonstrou associação de conjuntivite e ceratite com a positividade por B. canis, com um valor de odds ratio de 4,72 (I.C.95%=1,30-17,76) e Exato de Fisher de 0,0103; e 16,69 (I.C.95%=1,86-385,55) e Exato de Fisher de 0,0036, respectivamente. Os resultados obtidos neste estudo demonstraram a ocorrência da B. canis no município de...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Brucelose; PCR; Brucella canis; Cães; Cuiabá.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600016

Leptospirosis in native mixed-breed sheep slaughtered in a semiarid region of Brazil
Provedor de dados: Ciência Rural
Autores: Costa,Diego Figueiredo da; Silva,Aline Ferreira da; Brasil,Arthur Willian de Lima; Loureiro,Ana Paula Pereira; Santos,Fabrine Alexandre dos; Azevedo,Sergio Santos de; Lilenbaum,Walter; Alves,Clebert José.
ABSTRACT: Sheep breeding is an economic activity of great value for the Brazilian livestock industry, and infectious diseases can significantly compromise its productivity. The aim of this study was to detect Leptospira spp. in mixed breed sheep from a semiarid region of the State of Paraíba, Northeast Brazil. In total, 49 blood and renal tissue samples, as well as 25 urine samples, were collected from 49 sheep in the slaughter line. The microscopic agglutination test (MAT) was used to verify the presence of anti -Leptospira spp. antibodies in blood samples. Renal tissue and urine samples were analyzed by both PCR and bacterial culture. Sero reactivity was observed in 8.2% (4/49) of animals. Three renal samples were positive for leptospiral DNA by PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Small ruminants; PCR; Serology; Isolation; Slaughterhouse.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000200451

Detection of Toxoplasma gondii in a free-ranging giant anteater
Provedor de dados: Ciência Rural
Autores: Morgado,Thais Oliveira; Kagueyama,Francielle Cristina; Rosa,Janaina Marcela Assunção; Belizário,Melissa Debesa; Pacheco,Richard de Campos; Dutra,Valéria; Corrêa,Sandra Helena Ramiro; Paz,Regina Celia Rodrigues da.
ABSTRACT: Toxoplasmosis is caused by Toxoplasma gondii, an obligatory intracellular protozoan, which establishes acute and chronic infections in birds and mammals, including humans. This note reports, for the first time, the detection and sequencing of DNA from T. gondii in the peripheral blood of a young free range giant anteater (Myrmecophaga tridactyla). For the diagnosis, the following methods were used: polymerase chain reaction (PCR) and positive serology (1:800) by means of the modified agglutination test (MAT). Since this species may be consumed by humans and predated by wild felids, its importance is emphasized as a probable source of zoonotic infection, in addition to its possible participation in the infection enzootic cycle. Although,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myrmecophaga tridactyla; PCR; Modified agglutination test; Blood.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800501

Pythium insidiosum colitis in a dog: treatment and clinical outcome
Provedor de dados: Ciência Rural
Autores: Fujimori,Mahyumi; Lopes,Erika Rondon; Lima,Samara Rosolem; Paula,Daphine Ariadne Jesus de; Almeida,Arleana do Bom Parto Ferreira de; Colodel,Edson Moleta; Pescador,Caroline Argenta; Néspoli,Pedro Eduardo Brandini; Nakazato,Luciano; Dutra,Valéria; Souza,Roberto Lopes de; Sousa,Valéria Régia Franco.
ABSTRACT: The aim of this report is to describe the clinical, pathological and imaging findings and treatment of colitis caused by Pythium insidiosum in a canine presenting haematochezia and progressive weight loss. Through imaging, a thickening of the transverse and descending colon was observed. Histopathological analysis of the large intestine fragment revealed the presence of hyphae, confirmed by immunohistochemistry and PCR as P. insidiosum. Antifungal treatment with itraconazole implemented after partial surgical resection, resulted in control of the disease.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal pythiosis; PCR; Immunohistochemistry; Canine.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300526

Detecção de genes do cluster egc em Staphylococcus aureus isolados de alimentos de origem animal
Provedor de dados: Ciência Rural
Autores: Zocche,Fernando; Bastos,Caroline Peixoto; Silva,Wladimir Padilha da.
Os objetivos deste estudo foram detectar, por PCR, genes codificadores de enterotoxinas estafilocócicas, pertencentes ao cluster egc (genes seg, sei, selm, seln e selo) em Staphylococcus aureus isolados em diferentes alimentos de origem animal, e relacionar sua presença com a fonte de isolamento. Quarenta e uma cepas de S. aureus de diferentes origens (carne de frango, leite cru, embutidos cárneos e queijo) foram avaliadas por PCR, por meio da amplificação de um fragmento de 3375pb (denominado egc parcial), que foi utilizado como marcador da presença do cluster, e fragmentos de cada um dos genes pertencentes ao cluster egc. Há presença de genes do cluster egc em isolados de S. aureus isoladas em alimentos de origem animal; entretanto, diferentes genótipos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cluster de genes; Enterotoxinas estafilocócicas; Contaminação; Produtos de origem animal; PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000500021

Aspectos recentes da patogênese e diagnóstico da pleuropneumonia suína
Provedor de dados: Ciência Rural
Autores: Vaz,Clarissa Silveira Luiz; Silva,Sérgio Ceroni da.
A pleuropneumonia suína, causada por Actinobacillus pleuropneumoniae, é uma doença caracterizada pela apresentação fibrino-hemorrágica com pleurite adesiva. A enfermidade está presente em todos os países produtores de suínos, sendo responsável por prejuízos econômicos elevados. No Brasil e no mundo, diversos grupos vêm conduzindo estudos na busca por um melhor entendimento da doença e de sua epidemiologia. Avanços importantes foram obtidos, entre os quais a caracterização dos fatores de virulência, implicados na apresentação clínica da enfermidade; e a aplicação de novos métodos de diagnóstico. A difusão das técnicas de biologia molecular como ferramenta diagnóstica em Medicina Veterinária tem contribuindo para a identificação de Actinobacillus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pleuropneumonia suína; Actinobacillus pleuropneumoniae; Diagnóstico; PCR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000200049

Isolamento e PCR para detecção de Mollicutes em muco vaginal e sua associação com problemas reprodutivos em ovinos criados na região de Piedade, São Paulo, Brasil
Provedor de dados: Ciência Rural
Autores: Rizzo,Huber; Meira Junior,Enoch Brandão de Sousa; Oliveira,Rosângela Claret de; Yamaguti,Maurício; Buzinhani,Melissa; Timenetsky,Jorge; Gregory,Lilian.
Pesquisou-se Mycoplasma spp, Ureaplasma spp e Acholeplasma laidlawiii em amostras de muco vaginal de 60 ovinos, criados na região de Piedade no Estado de São Paulo, Brasil, que apresentavam ou não vulvovaginite no exame específico do sistema genital. A caracterização desses microrganismos baseou-se no cultivo e detecção do respectivo DNA pela Reação da Polimerase em Cadeia (PCR) com os primers para classe Mollicutes (GPO e MGSO), para o gênero Ureaplasma (UGPF e UGPS) e a espécie Acholeplasma laidlawii (UNI e ACH3). A presença de micoplasmas não foi associada com distúrbios do trato reprodutivo dos animais, entretanto todos os isolados obtidos de Ureaplasma spp foram provenientes de animais com distúrbios reprodutivos, sugerindo o possível envolvimento...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Micoplasmas; Ureaplasma; Ovelhas; Vulvovaginite.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000200024

Variabilidade genética em um ensaio de progênies de erva- mate (Ilex paraguariensis St.Hil.)
Provedor de dados: Ciência Rural
Autores: Vidor,Mario Angelo; Ruiz,César Pérez; Moreno,Santiago Vázquez; Floss,Paulo Alfonso.
A Epagri (Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina S.A) possui uma das maiores coleções brasileiras de erva-mate (Ilex paraguariensis St.Hil.). A partir de ensaios agronômicos preliminares, apresentaram destaque as procedências Barão de Cotegipe e Água Doce. O objetivo deste trabalho foi o de caracterizar geneticamente a variabilidade existente nos germoplasmas oriundos destas procedências. A técnica utilizada foi a de marcadores moleculares para amplificar o DNA, do tipo RAPD (Random Amplified Polymorphic DNA). Os resultados indicaram não ser possível diferenciar geneticamente as procedências entre si. Em função da distância genética, foi formado um grupo com quatro materiais ou germoplasmas e outro com os demais. Os diferentes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ilex paraguariensis; Variabilidade genética; Marcadores moleculares; DNA; RAPD; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000400006

A refrigeração no diagnóstico de Salmonella spp. utilizando o método microbiológico tradicional e reação em cadeia da polimerase em carcaças de frango
Provedor de dados: Ciência Rural
Autores: Possebon,Fabio Sossai; Costa,Luis Felipe Zuccolo Paschoal da; Yamatogi,Ricardo Seiti; Rodrigues,Marianna Vaz; Sudano,Mateus José; Pinto,José Paes de Almeida Nogueira.
A pesquisa de Salmonella em carcaças de aves tem mostrado resultados discrepantes, dependendo se as amostras foram colhidas ainda na indústria, imediatamente após o chiller ou no comércio varejista, quando se encontram submetidas à refrigeração por vários dias. Técnicas mais sensíveis, tais como a Reação em Cadeia pela Polimerase (PCR), podem fornecer dados importantes sobre o efeito do resfriamento sobre as células do patógeno, comparando seus resultados com os da metodologia microbiológica convencional (MC). Foram colhidas 130 carcaças de frango, sendo que 65 foram obtidas ainda na indústria (pós-chiller) e imediatamente analisadas e 65 carcaças embaladas e estocadas a 5°C por 72 horas (simulando o varejo), sendo então realizada a pesquisa do patógeno...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Salmonella; Frango; PCR; Refrigeração.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000100021

Prevalência de Streptococcus suis tipo 2 por meio da técnica de reação em cadeia da polimerase em suínos abatidos no Estado do Mato Grosso
Provedor de dados: Ciência Rural
Autores: Faria,Ana Carolina Silva de; Silva,Maria Cristina da; Oliveira Filho,João Xavier; Oliveira,Juçara Tinasi de; Paula,Daphine Ariadne Jesus de; Chitarra,Cristiane Silva; Nakazato,Luciano; Dutra,Valéria.
Streptococcus suis é um patógeno que a afeta a produção industrial de suínos em todo o mundo. É de extrema importância, pois está associado a doenças em suínos e humanos. O objetivo deste estudo foi determinar a prevalência do Streptococcus suis tipo 2 em 201 amostras de tonsilas de animais clinicamente sadios a partir da técnica de PCR. As amostras positivas foram submetidas à pesquisa do gene codificador do fator extracelular (ef). Os resultados demonstraram que a prevalência (23,38%) foi maior que em outro estudo recentemente realizado no mesmo Estado, indicando que a PCR é um método mais sensível em relação ao isolamento bacteriano. Houve baixa ocorrência do gene ef* (1,49%), o que mostra uma grande importância para população analisada, pois cepas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus suis tipo 2; PCR; Ef; Suínos.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000100021

Polymerase Chain Reaction and blood culture for diagnosis of canine sepsis
Provedor de dados: Ciência Rural
Autores: Silveira,Marcelo Marques da; Cândido,Stéfhano Luis; Santos,Karin Rinaldi dos; Maia,Maerle Oliveira; Souza,Roberto Lopes de; Sousa,Valéria Régia Franco; Almeida,Arleana do Bom Parto Ferreira de; Dutra,Valeria; Nakazato,Luciano.
ABSTRACT:  Sepsis is characterized by the presence of organ dysfunction secondary to the dysregulated systemic inflammatory response associated with an infection, and has high mortality rates. Traditional diagnostic techniques based on non-microbiological isolation are time-consuming and may delay treatment. Thus, this study aimed to compare bacterial and fungal broad-range polymerase chain reaction (PCR) and blood culture for diagnosis of sepsis in dogs. Blood samples from 88 dogs with suspected sepsis were analyzed by blood culture, and PCR to detect bacterial and fungal DNA. On blood culture, 20 (22.7%) samples tested positive for bacterial isolates; however, none tested positive for fungi. Through PCR analysis, bacterial DNA was detected in 46 (52.3%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood culture; PCR; Bacteremia; Fungemia.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600452

Isolation and identification of Mycobacterium bovis in milk from cows in northeastern Brazil
Provedor de dados: Ciência Rural
Autores: Ramos,Joelson Marcolino; Heinemann,Marcos Bryan; Ferreira Neto,José Soares; Filho,Antônio Francisco de Souza; Cárdenas,Nicolás Céspedes; Alves,Clebert José; Azevedo,Sérgio Santos de.
ABSTRACT: Milk samples from 16 cows that tested positive on the tuberculin test in the state of Paraíba, northeastern Brazil, were used for mycobacteria isolation and identification. Mycobacteria were isolated from five (31.25%) of the 16 milk samples; three samples were classified as M. bovis, and two as belonging to the Mycobacterium genus. This is probably the first study of isolation and identification of M. bovis in milk from cows in Northeastern Brazil, which suggests that humans are at risk of contamination by ingestion.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Mycobacterium bovis; Isolation; Milk; PCR; Cattle.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202166

Marcadores moleculares em estudos de caracterização de erva-mate (Ilex paraguariensis St.Hil.): o sabor
Provedor de dados: Ciência Rural
Autores: Vidor,Mario Angelo; Ruiz,César Pérez; Moreno,Santiago Vázquez; Floss,Paulo Alfonso.
A Epagri (Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina S.A) possui uma das maiores coleções brasileiras de erva-mate (Ilex paraguariensis St.Hil.). A partir de ensaios agronômicos preliminares, apresentaram destaque as procedências Barão de Cotegipe e Água Doce. Dentre essas, verificaram-se diferentes sabores (mais amargo e menos amargo), aparentemente, em função do tipo de folha (CL - curta e larga; LE - longa e estreita). O objetivo deste trabalho foi o de tentar associar os padrões obtidos mediante marcadores moleculares, com as procedências ou acessos e características fenotípicas desejáveis - altura de planta, diâmetro de copa, densidade folhar, sobrevivência das plantas e comportamento de rebrota -, dentro do caráter sabor em...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ilex paraguariensis; Marcadores moleculares; Variabilidade genética; PCR; RAPD; DNA; Biotecnologia.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000300008

An outbreak of caprine toxoplasmosis - investigation and case report
Provedor de dados: Ciência Rural
Autores: Ferreira Neto,José Mauricio; Ferreira,Fernanda Pinto; Miura,Ana Carolina; Almeida,Jonatas Campos de; Martins,Felippe Danyel Cardoso; Souza,Marielen de; Bronkhorst,Dalton Evert; Romanelli,Paulo Roberto; Pasquali,Aline Kuhn Sbruzzi; Santos,Hannah Lia Ettiene Peruch Lemos dos; Benitez,Aline do Nascimento; Caldart,Eloiza Teles; Zanella,Luiz Francisco; Freire,Roberta Lemos; Navarro,Italmar Teodorico.
ABSTRACT: The present study aimed to investigate an abortion outbreak in a dairy goat herd in the municipality of Arapoti, Parana, Brazil. At the beginning of the outbreak, blood samples were collected from 33 goats with clinical signs; later, of the whole goat herd, two cats and two dogs. Milk samples were collected from 78 lactating goats. Four environmental soil samples and four samples of feed residue from goat feeders were collected too. Immunofluorescence antibody test (IFA) was used for serodiagnosis, the molecular analysis was conducted by means of the polymerase chain reaction (PCR), for the isolation of the etiological agent the bioassay was used. The results of the IFA revealed that 76.53% (137/179) of the goats, two dogs and two cats were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma; Goats. IFA; PCR; Bioassay.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500500

IDENTIFICAÇÃO DOS GENES QUE CODIFICAM PARA A ENTEROTOXINA TERMOLÁBIL LT-II EM AMOSTRAS DE ESCHERICHIA COLI ISOLADAS DE BEZERROS COM DIARRÉIA NA REGIÃO DE JABOTICABAL, SP, BRASIL
Provedor de dados: Ciência Rural
Autores: Ugrinovich,Leila Aidar; De Ávila,Fernando Antonio; Oliveira,Maria Natália; Castro,Antonio Fernando Pestana de.
Examinando 52 espécimes fecais de bezerros com diarréia de fazendas da região de Jaboticabal, SP, Brasil, uma das amostras de Escherichia coli isoladas, quando analisada pela Reação em Cadeia da Polimerase (PCR), demonstrou a presença dos genes que codificam para a enterotoxina termolábil do tipo LT-II. Este é o primeiro relato de amostra de E. coli enterotoxigênica, isolada de bezerros com diarréia no Brasil, contendo os genes para codificação da enterotoxina LT-II. Encontram-se apenas citações de isolamento no Brasil de amostras de E. coli LT-II+ de alimentos de origem animal.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: E.coli; Enterotoxina LT-II; Detecção do gene; PCR; Brasil.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000200017

Aspectos fenotípicos, genotípicos e de diagnóstico da bactéria A. pleuropneumoniae
Provedor de dados: Ciência Rural
Autores: Costa,Mateus Matiuzzi da; Klein,Cátia Silene; Collares,Roberta Mattos; Vaz,Clarissa Silveira Luiz; Balestrim,Raquel; Schrank,Augusto; Silva,Sergio Ceroni da; Piffer,Itamar Antônio; Shrank,Irene Silveira.
A pleuropneumonia suína (PPS) provoca prejuízos significativos na suinocultura no mundo. O agente etiológico é a bactéria Actinobacillus pleuropneumoniae (App), que apresenta 15 sorotipos descritos, os quais variam consideravelmente em relação a sua patogenicidade. Nesse sentido, a precisa caracterização patotípica desta bactéria é de grande importância para a adoção de medidas de controle e profilaxia. O diagnóstico e a sorotipificação deste patógeno são realizados pelas técnicas microbiológicas convencionais. Entretanto, problemas nestes esquemas podem ser observados, especialmente em isolados de rebanhos sem histórico de PPS. No Brasil, diversos esforços vêm sendo aplicados no sentido de desenvolver técnicas moleculares que auxiliem no diagnóstico da...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: A. pleuropneumoniae; Diagnóstico; PCR; RDNA 16S; RAPD.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000400057

Contaminação ambiental e perfil toxigênico de Bacillus cereus isolados em serviços de alimentação
Provedor de dados: Ciência Rural
Autores: Soares,Celina Mara; Valadares,Geórgio Freesz; Azeredo,Raquel Monteiro Cordeiro de; Kuaye,Arnaldo Yoshiteru.
A avaliação da contaminação ambiental por Bacillus cereus foi realizada em 90 amostras de ar ambiente e em 96 amostras de superfícies de bancadas e de equipamentos, de dois restaurantes institucionais. O microrganismo foi detectado em 84,4% e 44,8% das amostras de ar ambiente e de superfícies, respectivamente. O potencial enterotoxigênico dos isolados foi investigado através da reação da polimerase em cadeia (PCR) para os genes hblA, hblD e hblC (que codificam a hemolisina BL) e para os genes nheA, nheB e nheC (que codificam a enterotoxina não hemolítica - NHE). De um total de 70 isolados investigados, 14,3% foram positivos para os três genes da HBL e 12,8% foram positivos para os três genes da NHE. A produção de NHE também foi verificada através do...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Segurança alimentar; Hemolisina; Enterotoxina diarréica; PCR; ELISA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000200033

Leishmania infantum infection in dogs from maroon communities in the Eastern Amazon
Provedor de dados: Ciência Rural
Autores: Silva,Andréia Ferreira da; Damasceno,Ágatha Rossanni Alves; Prado,Welton Seabra; Caldeira,Rossela Damasceno; Sampaio-Junior,Francisco Dantas; Farias,Diana Maria de; Silva,Laís Cristina Oliveira da; Guimarães,Ricardo José de Paula Souza e; Góes-Cavalcante,Gustavo; Scofield,Alessandra.
ABSTRACT: This study was designed to detect L. infantum infection in dogs and to evaluate the factors associated with canine visceral leishmaniasis in the maroon communities of Menino Jesus de Petimandeua and Itaboca in the municipality of Inhangapi, Pará, Brazil. Whole blood and intact skin samples were collected from 143 dogs, and a questionnaire was applied. L. infantum DNA was detected by polymerase chain reaction (PCR) using primers RV1 and RV2. Collection sites were georeferenced to obtain a spatial distribution of the residences visited and infected dogs. L. infantum DNA was detected in 8.4% (12/143) of the skin samples and in 1.4% (2/143) of the blood samples. On the risk map, three clusters were observed in Itaboca and one was observed in Menino...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania infantum; Maroon communities; Dog; PCR; Eastern Amazon.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100501

Campylobacter fetus subespécie fetus: abortamento e natimortalidade em ovinos
Provedor de dados: Ciência Rural
Autores: Gressler,Letícia Trevisan; Kirinus,Jackeline Karsten; Machado,Gustavo; Libardoni,Felipe; Vargas,Agueda Castagna de.
A bactéria do gênero Campylobacter está comumente envolvida em surtos de abortos ovinos em muitos países. No Brasil, até o presente momento, ainda não houve relato sobre a ocorrência de aborto em ovinos causado pelo C. fetus, subespécie fetus (C. fetus ssp. fetus). No presente trabalho, relata-se a detecção deste agente em fetos e natimortos ovinos naturalmente infectados. Desse modo, alerta-se para a possibilidade de novos casos de aborto em ovinos causados por C. fetus ssp. fetus.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Aborto; Natimorto; Campylobacter fetus subesp. fetus; Ovino; PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000400020

Análise da contaminação por Salmonella em ovos do tipo colonial através da reação em cadeia da polimerase
Provedor de dados: Ciência Rural
Autores: Flôres,Maristela Lovato; Nascimento,Vladimir Pinheiro do; Kader,Ivonyr Irene Tróglio Abdel; Cardoso,Martha; Santos,Luciana Ruschel dos; Lopes,Rui Fernando Félix; Wald,Vera Beatriz; Barbosa,Taylor Marcelo Corrêa.
A identificação de poedeiras comerciais infectadas por salmonelas tem sido um dos pontos fortes da profilaxia e conseqüente redução de surtos de salmonelose em humanos associados ao consumo de ovos, sendo que a análise dos ovos pode ser mais um dos pontos de detecção da infecção, que, muitas vezes, cursa sem sinais clínicos. A Reação em Cadeia da Polimerase (PCR) parece ser uma estratégia útil para detecção de Salmonella, pois vários autores têm utilizado a PCR para verificar a presença da bactéria em carnes, fezes, tecidos, sangue, leite e ovos, com diferentes metodologias de manipulação das amostras. Foram analisados 360 ovos, procedentes de dez propriedades rurais, produtoras de ovos tipo colonial, no distrito de Camobi, em Santa Maria - RS. Os ovos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonelas; Ovos coloniais; PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000300025

Inquérito sorológico e molecular da brucelose canina no município de Natal, Estado do Rio Grande do Norte
Provedor de dados: Ciência Rural
Autores: Fernandes,Annielle Regina da Fonseca; Fernandes,Ademilde Gomes; Rotondano,Tereza Emmanuelle de Farias; Alves,Clebert José; Kim,Pomy de Cássia Peixoto; Mota,Rinaldo Aparecido; Azevedo,Sérgio Santos de.
O objetivo do presente trabalho foi determinar a ocorrência de anticorpos anti-Brucella rugosa e anti-Brucella lisa em cães do município de Natal, Estado do Rio Grande do Norte, Brasil, bem como identificar fatores de risco associados à positividade e realizar a detecção molecular em animais soropositivos. Foram utilizados soros sanguíneos de 416 cães atendidos em clínicas veterinárias durante o período de março a novembro de 2011. Para o diagnóstico sorológico da infecção por Brucella rugosa, foi empregada a prova de imunodifusão em gel de ágar (IDGA), utilizando antígeno de lipopolissacarídeos e proteínas de Brucella ovis, amostra Reo 198 e, para o diagnóstico da infecção por Brucella lisa, foi utilizado o teste do antígeno acidificado tamponado (AAT)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucelose canina; Cães; IDGA; PCR; Brasil.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000900015

Ocorrência e diferenciação de espécies de Listeria spp. em salsichas tipo hot dog a granel e em amostras de carne moída bovina comercializadas no Distrito Federal
Provedor de dados: Ciência Rural
Autores: Andrade,Rafael Rocha de; Silva,Patrícia Helena Caldeira da; Souza,Nara Rúbia; Murata,Luci Sayori; Gonçalves,Vitor Salvador Picão; Santana,Angela Patrícia.
Listeria monocytogenes é um patógeno relevante veiculado por alimentos. Sua identificação precisa é importante para a correta determinação do risco associado à ingestão do alimento. O objetivo deste trabalho foi verificar a ocorrência das espécies de Listeria spp. em amostras de salsichas tipo hot dog e carne moída bovina, comercializadas a granel no Distrito Federal. Foram analisadas 162 amostras, sendo 127 de salsichas tipo hot dog e 35 amostras de carne moída bovina. O isolamento e a identificação do gênero foram feitos por metodologia convencional e a distinção das espécies foi verificada por kit bioquímico específico (API-Listeria®) e por análise de restrição de fragmentos da reação em cadeia da polimerase (RFLP-PCR) do gene 23 rRNA. Foram isoladas 26...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Produtos cárneos; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000100024

Molecular detection of Trypanosoma evansi (Kinetoplastida: Trypanosomatidae) in procyonids (Carnivora: Procyonidae) in Eastern Amazon, Brazil
Provedor de dados: Ciência Rural
Autores: Magalhães-Matos,Paulo Cesar; Cunha-Santos,Rafaelle; Sousa,Paulo Geovani Silva; Sampaio-Júnior,Francisco Dantas; Barros,Flávia de Nazaré Leite; Mourão,Fábio Rodrigo Paixão; Oliveira,Wanessa Batista Lima; Gabriel,Áurea Martins; Monteiro,Silvia Gonzalez; Góes-Cavalcante,Gustavo; Scofield,Alessandra.
ABSTRACT: The present study aimed to diagnose the natural infection of captive and free-living procyonids with Trypanosoma evansi in the states of Amapá and Pará, Brazil. From February 2012 to August 2013, whole blood samples and blood smears were obtained from 45 free-living procyonids and from nine procyonids kept in captivity in wild life refuges and zoobotanical parks in the states of Amapá and Pará. Whole blood samples were collected and kept at -20ºC for the detection of T. evansi DNA by PCR using the RoTat 1.2 forward and RoTat 1.2 reverse primers. In addition, the blood smears were processed and examined for the presence of trypomastigote forms of T. evansi. T. evansi DNA was detected in 18.52% (10/54) of the procyonids, namely, in captive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma evansi; PCR; Procyonidae; Eastern Amazon.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400663

Leishmania chagasi in dogs from the city of Jaciara, Mato Grosso, Brazil
Provedor de dados: Ciência Rural
Autores: Lazari,Patrícia; Almeida,Arleana do Bom Parto Ferreira de; Dutra,Valéria; Nakazato,Luciano; Dias,Álvaro Felipe de Lima Ruy; Brito,Veruska Nogueira de; Oliveira,Cenita Maria; Sousa,Valéria Régia Franco.
ABSTRACT: The aim of this study was to identify the Leishmania species in Jaciara dogs; visceral leishmaniasis (VL) is endemic in the county of Jaciara. A comparison among the PCR results for the spleen, bone marrow, skin and blood of 101 dogs with VL-reactive serum were evaluated. Spleen tissue showed the highest detection percentage, followed by bone marrow. In 97 (96.04%) dogs, the Leishmania DNA detected was confirmed as L. chagasi, emphasizing the importance of aetiological identifications in sympatric areas with other trypanosomatids.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Visceral leishmaniasis; PCR; Different tissues.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200315

Expression analysis of genes involved in the synthesis of oleic and linoleic acids in Jatropha cinerea seeds from Northwestern Mexico
Provedor de dados: Ciência Rural
Autores: Lovio-Fragoso,Jose Pablo; Medina-Juárez,Luis Angel; Gamez-Meza,Nohemi; Martínez,Octavio; Hernández-Oñate,Miguel Ángel; Hayano-Kanashiro,Corina.
ABSTRACT:  Plants belonging to genus Jatropha has arisen interest because of its high oil content that could be used to produce biodiesel. It is also widely reported that the main fatty acids in Jatropha oilseed are oleic and linoleic acids. However, there are scarce studies related to native species of Jatropha from Northwestern Mexico which are adapted to arid conditions, and the expression of genes involved in fatty acid synthesis for these species is still unknown. Therefore, the aim of this study was to analyze the expression of five genes, ACP1, KASII, D9SD, FAD2-1 and FAD2-2, which are involved in the oleic and linoleic acids synthesis in mature wild-seeds of Jatropha cinerea, a native species from Sonoran Desert, using semi-quantitative RT-PCR. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatty acids biosynthesis; PCR; KASII; FAD2-1.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100405

MÉTODOS DE EXTRAÇÃO DE DNA PARA A DETECÇÃO DE Salmonella EM OVOS DE GALINHAS, COM E SEM CASCA, ATRAVÉS DA REAÇÃO EM CADEIA PELA POLIMERASE
Provedor de dados: Ciência Rural
Autores: Flôres,Maristela Lovato; Nascimento,Vladimir Pinheiro do; Kader,Ivonyr Irene Troglio Abdel; Santos,Luciana Ruschel dos; Pontes,Alexandre Pontes; Salle,Carlos Tadeu Pippi; Lopes,Rui Fernando Felix.
O diagnóstico microbiológico de Salmonella sp em amostras de alimentos é demorado, com cinco diferentes etapas, levando cerca de 120 horas até o resultado final. A utilização da técnica da Reação em Cadeia pela Polimerase (PCR) pode diminuir esse período, porém sofre influência de substâncias presentes na amostra que afetam a reação. O objetivo deste trabalho foi comparar dois métodos de extração de DNA, a extração por tratamento térmico e a pelo fenol-clorofórmio, em amostras de 100 ovos de galinhas domésticas artificialmente contaminados com uma cepa de Salmonella enterica sorovar typhimurium em fase estacionária. O material obtido com as extrações foi submetido à PCR, utilizando-se um par de iniciadores que amplificam um fragmento de 284pb do gene InvA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Ovos; Extração; DNA; PCR.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000200020

Distribuição de genes cry de Bacillus thuringiensis isolados de solos do Estado do Rio Grande do Sul, Brasil
Provedor de dados: Ciência Rural
Autores: Pinto,Laura Massochin Nunes; Fiuza,Lidia Mariana.
A bactéria Bacillus thuringiensis (Bt) caracteriza-se pela produção de proteínas tóxicas a representantes de diversas ordens de insetos, as quais são codificadas por genes cry. Devido a esta característica, mais de 40.000 cepas de Bt foram isoladas e cerca de 190 genes cry, caracterizados. Como os dados sobre Bt são limitados no Rio Grande do Sul, essa pesquisa objetivou avaliar a distribuição de seis famílias de genes cry de Bt, desse estado, que codificam proteínas ativas contra insetos-praga. O perfil dos 46 isolados de solos do Rio Grande do Sul foi avaliado, por PCR com os primers que detectam os genes cry1, cry2, cry3, cry7 cry8 e cry9 e suas respectivas proteínas foram analisadas por SDS-PAGE a 10%. A presença de genes cry9 foi detectada em 47,82%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Controle biológico; Entomopatógenos; Bactérias; PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000400018

An improved method for genomic DNA extraction from strawberry leaves
Provedor de dados: Ciência Rural
Autores: Nunes,Claudinéia Ferreira; Ferreira,Juliano Lino; Fernandes,Mônique Carolina Nunes; Breves,Sâmera de Souza; Generoso,Andressa Leal; Soares,Bárbara Dantas Fontes; Dias,Mário Sérgio Carvalho; Pasqual,Moacir; Borem,Aluízio; Cançado,Geraldo Magela de Almeida.
Several extraction methods of genomic DNA for identification and characterization of genetic diversity in different plant species are routinely applied during molecular analysis. However, the presence of undesirable compounds such as polyphenols and polysaccharides is one of the biggest problems faced during the isolation and purification of high quality DNA in plants. Therefore, achievement of fast and accurate methods for DNA extraction is crucial in order to produce pure samples. Leaves of strawberry genotypes (Fragaria ananassa) have high contents of polysaccharides and polyphenols which increase the sample viscosity and decrease the DNA quality, interfering with the PCR performance. Thereby, in this study we evaluated the quality and amount of genomic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fragaria ananassa; Genomic DNA; Polivinilpirrolidone; Lyophilization; PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800014

Identificação bioquímica e molecular de Lactobacillus spp. isolados do íleo de frangos de corte tratados ou não com antimicrobianos
Provedor de dados: Ciência Rural
Autores: Zanini,Surama Freitas; Mussi,Jamili Maria Suhet; Zanini,Marcos Santos; Sousa,Dyeime Ribeiro; Pessotti,Bruna Mirelly de Souza; Damasceno,João Damasceno Lopes Martins; Silva,Maria Aparecida da.
Objetivou-se caracterizar bioquimicamente e molecularmente as espécies de Lactobacillus spp. isoladas do íleo de frangos de corte tratados ou não com antimicrobianos. Utilizou-se 400 pintos de corte alojados em 25 boxes de 2m² (16 aves/boxe), distribuídos em um delineamento inteiramente casualizado, em grupos de cinco tratamentos e cinco repetições: dieta sem promotor de crescimento; dieta com promotor de crescimento; dieta com 0,4% de óleo de aroeira-vermelha (OAV); dieta com 200mg de vitamina E kg-1; dieta com 0,4% OAV e 200mg de vitamina E kg-1. Após a caracterização fenotípica do gênero Lactobacillus, foram identificadas, em ambas as metodologias, 100 amostras de Lactobacillus spp. sendo 20 amostras por tratamento. Os resultados bioquímicos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Frangos de corte; Antibióticos; Schinus terebinthifolius Raddi; Óleo essencial; PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000900021

Equine nasopharyngeal cryptococcoma due to Cryptococcus gattii
Provedor de dados: Ciência Rural
Autores: Cruz,Raquel Aparecida Sales da; Reis,Matheus de Oliveira; Leite Filho,Ronaldo Viana; Gonçalves,Maiara Aline; Spanamberg,Andréia; Sonne,Luciana; Ferreiro,Laerte; Pavarini,Saulo Petinatti; Driemeier,David.
ABSTRACT: Cryptococcus gattii is often associated with pulmonary and systemic infections in humans and animals. In this research we report a case of nasopharyngeal cryptococoma caused by C. gatti in an equine. A 10-year-old mare presented a mass obstructing the oropharynx. Macroscopically the mass was asymmetric, and was attached to the ethmoidal sinuses and obstructed the oropharynx. Histopathological examination of the mass revealed multiple yeast cells ranging from spherical to oval, 4-8μm in diameter, with some of them showing narrow base polar budding. Cryptococcus gattii growth in mycological culture (Sabouraud Dextrose Agar) and was L-canavanine-glycine-bromothymol blue Agar positive. The molecular identification confirmed the isolate as C. gattii...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycotic diseases; Crytococcosis; Equine; PCR; Cryptococcus gattii.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001000551

Distribuição e identificação de Phakopsora pachyrhizi, agente causal da ferrugem da soja no estado de Minas Gerais
Provedor de dados: Ciência e Agrotecnologia
Autores: Sousa,Patrícia Ferreira Cunha; Alves,Eduardo; Castro,Hilário Antônio de; Souza,Paulo Estevão de; Almeida,Álvaro Manuel Rodrigues de; Hochenbach,Silvana Regina.
A ferrugem da soja é causada por duas espécies: Phakopsora pachyrhizi e P. meibomiae. No Brasil, a doença tem sido responsável por grandes perdas dessa leguminosa nas últimas safras. Um levantamento na distribuição do patógeno e a identificação correta da espécie prevalecente nas regiões produtoras no Brasil, tornou-se necessário para orientar o planejamento das medidas de controle. A caracterização molecular baseada em PCR tem se mostrado uma ferramenta útil para atender a essa necessidade. Neste estudo, foi realizada a coleta de 86 amostras de folhas infectadas com urediniósporos de Phakopsora, nas principais regiões produtoras de soja do Estado de Minas Gerais (Triângulo, Alto Paranaíba, Noroeste, Sudoeste e Sul), envolvendo 22 municípios. A extração de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Phakopsora pachyrhizi; Levantamento.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542007000300011

Constatação de Xylella fastidiosa em pecíolos e hipocotilos de cafeeiro com sintomas de mancha manteigosa
Provedor de dados: Ciência e Agrotecnologia
Autores: Lins,Severina Rodrigues de Oliveira; Abreu,Mário Sobral de; Alves,Eduardo; Barbosa,Juliana Franco; Souza,Ricardo Magela de.
A mancha manteigosa tem afetado um grande número de plantas de cafeeiro em condições de campo. Sua causa tem sido atribuída a Colletotrichum gloeosporioides, entretanto a sintomatologia da doença na folha não tem sido reproduzida. Neste estudo, relata-se pela primeira vez a associação de Xylella fastidiosa, agente da atrofia dos ramos de cafeeiro, com pecíolo de folhas e hipocótilos obtidos a partir de sementes de plantas com sintomas da mancha manteigosa, através de estudos ultra-estruturais em Microscópio Eletrônico de Varredura (MEV), bem como por PCR (Polymerase Chain Reaction). Para o estudo foram realizados três ensaios. No primeiro, coletaram-se folhas com sintomas de mancha manteigosa e assintomáticas em duas localidades as quais foram preparadas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mancha manteigosa; Xylella fastidiosa; Microscopia eletrônica de varredura; PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000100006

Levantamento da incidência de Leifsonia xyli subsp. xyli em plantios de cana-de-açúcar do noroeste do Paraná
Provedor de dados: Ciência e Agrotecnologia
Autores: Marcuz,Fernanda Santos; Souto,Eliezer Rodrigues de; Vieira,Rafael Augusto; Marrafon,Marco Aurélio; Barboza,Antônio Augusto Lazarini; Daros,Edelclaiton.
O raquitismo-da-soqueira, causado por Leifsonia xyli subsp. xyli é considerado uma das mais importantes doenças bacterianas da cana-de-açúcar (Saccharum spp.). Para verificar a possível ocorrência de Leifsonia xyli subsp. xyli na região noroeste do Paraná, foi realizado um levantamento em 32 áreas de plantio de duas usinas, utilizando-se o método sorológico "Dot blot" e um protocolo baseado na reação em cadeia da polimerase. As amostras foram coletadas em áreas cultivadas com 8 variedades e 2 clones, a partir de plantas em diferentes épocas de corte, perfazendo um total de 1395 amostras. Constatou-se a presença da bactéria no fluído fibrovascular de plantas da variedade SP77-5181 coletado em uma das propriedades, com índice de incidência de 23%. Esses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Raquitismo-da-soqueira; Sorologia; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000700041