Consulta: subjectFacets:"AFLP"
Registros recuperados: 66
Data/hora: 29/05/2024 03:57:46

Análisis preliminar de la variabilidad genética de Escallonia alpina y E. rubra (Escalloniaceae)
Provedor de dados: 66
Autores: Morello,Santiago; Giussani,Liliana M; Sede,Silvana M.
Escallonia alpina y E. rubra (Escalloniaceae) comparten su área de distribución en el sur de los Andes y se diferencian por pocos caracteres morfológicos diagnósticos, tales como la disposición de las flores y la presencia y tipo de glándulas; sin embargo, en algunas poblaciones no es posible asignar los individuos a una u otra especie. Además, se han reconocido y descrito como híbridos varias formas de transición entre ambas. El objetivo de este trabajo es examinar la variabilidad genética poblacional de Escallonia alpina y E. rubra, y analizar si se corresponde con la variabilidad morfológica observada. Se estudió la morfología en 39 individuos de cinco poblaciones sobre los que también se realizó un análisis de AFLP. Se investigó la relación entre los...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Escallonia; Patagonia; Sudamérica austral.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0011-67932013000200004

Varianza genética y mapeo molecular de rendimiento y calidad nutricional en familias de medios hermanos en Medicago sativa.
Provedor de dados: 32
Autores: Galicia Juárez, Marisol.
La alfalfa (Medicago sativa L.) es  considerada como el cultivo forrajero de mayor importancia en la nutrición de animales domésticos, y se siembra en todo el mundo. A pesar de existir una amplia gama de variedades de alfalfa  sembradas en México hay pocos estudios acerca de la variabilidad genética dentro y entre poblaciones criollas, por tanto esta investigación tuvo el objetivo de estimar la variabilidad genética de caracteres cuantitativos agronómicos y de calidad de nutrientes considerando ocho poblaciones de alfalfa bajo un diseño de familias de medios hermanos a partir de marcadores AFLPs (EcoRI y Mse). Se empleó un diseño lattice rectangular 8 x 10 con tres repeticiones y mediciones repetidas a través del tiempo. El análisis de los componentes de...
Palavras-chave: Medicago sativa; Familia de medios hermanos; AFLP; Variabilidad genética; Maestría; Ganadería.
Ano: 2012
URL: http://hdl.handle.net/10521/769

Evaluation of cassava plants generated by somatic embryogenesis at different stages of development using molecular markers
Provedor de dados: 69
Autores: Osorio,Mayra; Gámez,Elizabeth; Molina,Sandy; Infante,Diógenes.
Background: Cassava (Manihot esculenta Crantz) is a crop that is high in carbohydrates in the roots and in protein in the leaves, important for both human consumption and animal feed, and also has a significant industrial use for its starches. In this study we evaluated the genetic variability with molecular markers in different stages in micropropagated plants from somatic embryos of Venezuelan native clone 56. Results: Three markers were used: ISTR, AFLP and SSR, finding that ISTR showed the highest polymorphism among individuals tested. With AFLP a high similarity between the evaluated individuals was observed and with SSR total monomorphism was seen. Using cluster analysis it was found that individuals from an embryo labeled as fasciated at the...
Tipo: Journal article
Palavras-chave: AFLP; Genetic stability; ISTR; SSR; UPGMA.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400003

Assessment of genetic diversity in sesame (Sesamum indicum L.) detected by Amplified Fragment Length Polymorphism markers
Provedor de dados: 69
Autores: Ali,Ghulam M; Yasumoto,Sirato; Seki-Katsuta,Masumi.
Sesame (Sesamum indicum L.) is one of the oldest oil crops and is widely cultivated in Asia and Africa. To determine the level of genetic diversity in relation to geographical origins and morphological characteristics, a total of 96 accessions have been collected from different parts of the world and were analyzed using AFLP techniques. Twenty-one primer pairs generated a total of 445 bands and among them 157 (35%) were polymorphic. Using UPGMA clustering analysis method based on the similarity coefficient, accessions were separated into two major groups. The first group mostly consists of Eastern Asian origin and another group consists of South Asian origin. Sub-clusters separated the accessions and form distinct diversity among groups. Considering the...
Tipo: Journal article
Palavras-chave: AFLP; Genetic diversity; Geographical origin; Morphology; Sesame.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100002

Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker
Provedor de dados: 69
Autores: Tiwari,Jagesh Kumar; Saurabh,Shaumaya; Chandel,Poonam; Singh,Bir Pal; Bhardwaj,Vinay.
Background: Genetic and epigenetic changes (DNA methylation) were examined in the tissue-culture propagated interspecific potato somatic hybrids between dihaploid Solanum tuberosum and S. pinnatisectum. Amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP) were applied to detect the genetic and epigenetic changes, respectively in the somatic hybrids mother plants (1st cycle) and their regenerants (30th cycles sub-cultured). Results: To detect genetic changes, eight AFLP primer combinations yielded a total of 329 scorable bands of which 49 bands were polymorphic in both mother plants and regenerants. None of the scorable bands were observed in term of loss of original band of mother plant or gain of novel band...
Tipo: Journal article
Palavras-chave: AFLP; DNA methylation; In vitro propagation; MSAP; Potato; Somatic hybrids.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600005

Cotton genetic diversity study by AFLP markers
Provedor de dados: 69
Autores: Murtaza,Naveed.
Amplified fragmentlength polymorphism (AFLP) markers have been used to ascertain the intensity of inherent diversity and relatedness in cotton (Gossypium spp.) plants. The effectiveness of this method to distinguish inter and intra specific difference in cotton could be handy in cultivar recognition and in marker assisted parental selection tool for plant breeders. Twenty cotton cultivars belonging to Gossypium hirsutum L., and G. arborium L. from the Pakistan and US origin were used for AFLP based genetic diversity estimates. The objective of this study was to assess the level of genetic variation among some cotton cultivars belonging to the old and new world species of cotton. Four EcoRI-MseI primer-pair combinations were used forthe AFLP analysis. The...
Tipo: Journal article
Palavras-chave: AFLP; Cluster analysis; Cotton; Genetic diversity; PCR.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400013

Agronomic and molecular analyses for the characterisation of accessions in Tunisian olive germplasm collections
Provedor de dados: 69
Autores: Taamalli,Wael; Geuna,Filippo; Banfi,Riccardo; Bassi,Daniele; Daoud,Douja; Zarrouk,Mokhtar.
In this study, a total of 14 agronomic traits, five AFLP primer combinations and ten SSR loci were used to describe and to classify a group of Tunisian olive genotypes into groups based on molecular profiles and agronomic traits. The analysis of variance of the agronomical data revealed significant differences among accessions for all measured traits. The mean phenotypic dissimilarity (0.34 with a range of 0.08-0.6) was low in comparison to dissimilarity calculated using AFLP (0.50 with a range of 0.16-0.70) and SSR markers (0.76 with a range 0.35-0.94). The correlation between the agronomical dissimilarity matrix and the matrices of genetic dissimilarity based on SSR and AFLP markers was very weak: 0.156 (p = 0.05) and 0.185 (p = 0.05), respectively. The...
Tipo: Journal article
Palavras-chave: Agronomic traits; AFLP; Olea europaea; SSR.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500002

WITHIN-POPULATION GENETIC DIVERSITY OF CLIMBING PLANTS AND TREES IN A TEMPERATE FOREST IN CENTRAL CHILE
Provedor de dados: 73
Autores: Torres-Díaz,Cristian; Ruiz,Eduardo; Salgado-Luarte,Cristian; Molina-Montenegro,Marco A; Gianoli,Ernesto.
The climbing habit is a key innovation in angiosperm evolution: climbing plant taxa have greater species richness than their non-climbing sister groups. It is considered that highly diversified clades should show increased among-population genetic differentiation. Less clear is the expected pattern regarding within-population genetic diversity in speciose lineages. We tested the hypothesis of greater within-population genetic diversity in climbing plants compared to trees in a temperate forest in central Chile. The assumption underlying this hypothesis is that higher among-population differentiation in climbers compared to trees should reflect higher genetic diversity as well. AFLP markers from 167 individual plants from 14 species (seven climbers and...
Tipo: Journal article
Palavras-chave: AFLP; Climbers; Evolution; Genetic variability; Key innovation.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432013000100005

AFLP marker linked to water-stress-tolerant bulks in barley (Hordeum vulgare L.)
Provedor de dados: 74
Autores: Altinkut,A.; Kazan,K.; Gozukirmizi,N.
The amplified fragment length polymorphism (AFLP) assay is an efficient method for the identification of molecular markers, useful in the improvement of numerous crop species. Bulked Segregant Analysis (BSA) was used to identify AFLP markers associated with water-stress tolerance in barley, as this would permit rapid selection of water-stress tolerant genotypes in breeding programs. AFLP markers linked to water-stress tolerance was identified in two DNA pools (tolerant and sensitive), which were established using selected F2 individuals resulting from a cross between water-stress-tolerant and sensitive barley parental genotypes, based on their paraquat (PQ) tolerance, leaf size, and relative water content (RWC). All these three traits were previously shown...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bulked segregant analysis; Drought; Hordeum vulgare; AFLP; Paraquat.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100013

SSR and AFLP based genetic diversity of soybean germplasm differing in photoperiod sensitivity
Provedor de dados: 74
Autores: Singh,Ram K.; Bhatia,Virendra S.; Bhat,K.V.; Mohapatra,Trilochan; Singh,Nagendra K.; Bansal,Kailash C.; Koundal,K.R.
Forty-four soybean genotypes with different photoperiod response were selected after screening of 1000 soybean accessions under artificial condition and were profiled using 40 SSR and 5 AFLP primer pairs. The average polymorphism information content (PIC) for SSR and AFLP marker systems was 0.507 and 0.120, respectively. Clustering of genotypes was done using UPGMA method for SSR and AFLP and correlation was 0.337 and 0.504, respectively. Mantel's correlation coefficients between Jaccard's similarity coefficient and the cophenetic values were fairly high in both the marker systems (SSR = 0.924; AFLP = 0.958) indicating very good fit for the clustering pattern. UPGMA based cluster analysis classified soybean genotypes into four major groups with fairly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photoperiod response; SSR; AFLP; Genetic diversity; Soybean.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200019

Association between molecular markers and behavioral phenotypes in the immatures of a butterfly
Provedor de dados: 74
Autores: De Nardin,Janaína; Buffon,Vanessa; Revers,Luís Fernando; de Araújo,Aldo Mellender.
Abstract Newly hatched caterpillars of the butterfly Heliconius erato phyllis routinely cannibalize eggs. In a manifestation of kin recognition they cannibalize sibling eggs less frequently than unrelated eggs. Previous work has estimated the heritability of kin recognition in H. erato phyllis to lie between 14 and 48%. It has furthermore been shown that the inheritance of kin recognition is compatible with a quantitative model with a threshold. Here we present the results of a preliminary study, in which we tested for associations between behavioral kin recognition phenotypes and AFLP and SSR markers. We implemented two experimental approaches: (1) a cannibalism test using sibling eggs only, which allowed for only two behavioral outcomes (cannibal and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kin discrimination; Caterpillar-egg cannibalism; Lepidoptera; Heliconius; AFLP.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200243

Patterns of genetic diversity in southern and southeastern Araucaria angustifolia (Bert.) O. Kuntze relict populations
Provedor de dados: 74
Autores: Souza,Maria Isabel Ferreira de; Salgueiro,Fabiano; Carnavale-Bottino,Mariana; Félix,Durvalina Benedita; Alves-Ferreira,Marcio; Bittencourt,Juliana Vitoria Messias; Margis,Rogério.
Habitat fragmentation and a decrease in population size may lead to a loss in population genetic diversity. For the first time, the reduction in genetic diversity in the northernmost limit of natural occurence (southeastern Brazil) of Araucaria angustifolia in comparison with populations in the main area of the species continuous natural distribution (southern Brazil), was tested. The 673 AFLPs markers revealed a high level of genetic diversity for the species (Ht = 0.27), despite anthropogenic influence throughout the last century, and a decrease of H in isolated populations of southeastern Brazil (H = 0.16), thereby indicating the tendency for higher genetic diversity in remnant populations of continuous forests in southern Brazil, when compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucariaceae; Relict populations; Araucaria forest; Population genetics; AFLP.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300018

AFLP marker analysis revealing genetic structure of the tree Parapiptadenia rigida (Benth.) Brenan (Leguminosae-Mimosoideae) in the southern Brazilian Tropical Rainforest
Provedor de dados: 74
Autores: Souza,Laís Bérgamo de; Ruas,Eduardo A.; Rodrigues,Luana A.; Ruas,Claudete F.; Ruas,Paulo M.
Parapiptadenia rigida is a tropical early secondary succession tree characteristic of the Tropical Atlantic Rainforest. This species is of great ecological importance in the recovery of degraded areas. In this study we investigated the variability and population genetic structure of eight populations of P. rigida. Five AFLP primer combinations were used in a sample of 159 individuals representing these eight populations, rendering a total of 126 polymorphic fragments. The averages of percentage of polymorphic loci, gene diversity, and Shannon index were 60.45%, 0.217, and 0.322, respectively. A significant correlation between the population genetic variability and the population sizes was observed. The genetic variability within populations (72.20%) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tropical tree; Genetic diversity; Population genetics; Conservation; AFLP.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400012

Genetic relationships among Arachis species based on AFLP
Provedor de dados: 74
Autores: Gimenes,Marcos A.; Lopes,Catalina R.; Valls,Jose F.M.
Amplified Fragment Length Polymorphism (AFLP) was used to establish the genetic relationships among 20 species from seven of the nine sections of genus Arachis. The level of polymorphism among nine accessions of the cultivated peanut, A. hypogaea L., was also evaluated. Three combinations of primers were used to amplify the AFLPs. The fragments were separated in 6% denaturing acrylamide gels. A total of 408 fragments were analyzed. An average of 135.3 fragments per primer combination were scored, and the largest number of fragments was 169 using primer combination Eco RI - ACC / Mse I - CTG, while the lowest was 108, with Eco RI - ACT / Mse I - CTT. In general, the genetic relationships established using AFLPs agreed with the classification established...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Arachis; Peanut; Taxonomic relationship.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300017

Comparison of similarity coefficients used for cluster analysis with dominant markers in maize (Zea mays L)
Provedor de dados: 74
Autores: Meyer,Andréia da Silva; Garcia,Antonio Augusto Franco; Souza,Anete Pereira de; Souza Jr.,Cláudio Lopes de.
The objective of this study was to evaluate whether different similarity coefficients used with dominant markers can influence the results of cluster analysis, using eighteen inbred lines of maize from two different populations, BR-105 and BR-106. These were analyzed by AFLP and RAPD markers and eight similarity coefficients were calculated: Jaccard, Sorensen-Dice, Anderberg, Ochiai, Simple-matching, Rogers and Tanimoto, Ochiai II and Russel and Rao. The similarity matrices obtained were compared by the Spearman correlation, cluster analysis with dendrograms (UPGMA, WPGMA, Single Linkage, Complete Linkage and Neighbour-Joining methods), the consensus fork index between all pairs of dendrograms, groups obtained through the Tocher optimization procedure and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic divergence; Heterotic groups; AFLP; RAPD; Multivariate analysis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100014

Genetic similarity among soybean (Glycine max (L) Merrill) cultivars released in Brazil using AFLP markers
Provedor de dados: 74
Autores: Bonato,Ana Lídia V.; Calvo,Eberson S.; Geraldi,Isaias O.; Arias,Carlos Alberto A.
Genetic similarity among soybean genotypes was studied by applying the amplified fragment length polymorphism (AFLP) technique to 317 soybean cultivars released in Brazil from 1962 to 1998. Genetic similarity (GS) coefficients were estimated using the coefficient of Nei and Li (Nei and Li 1979), and the cultivars were clustered using the unweighted pair-group method with averages (UPGMA). The parentage coefficients of 100 cultivars released between 1984 and 1998 were calculated and correlated with the genetic similarity obtained by the markers. The genetic similarity coefficients varied from 0.17 to 0.97 (x = 0.61), with 56.8% of the coefficients being above 0.60 and only 9.7% equal to or less than 0.50. The similarity coefficients have remained constant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Genetic similarity; Molecular markers; Parentage coefficient; Soybean.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400019

Use of AFLP and RAPD molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the Patagonian Bromus setifolius complex
Provedor de dados: 74
Autores: García,Ana M.; Schrauf,Gustavo E.; González,Graciela; Poggio,Lidia; Naranjo,Carlos A.; Dupal,Marck P.; Spangenberg,Germán C.; Forster,John W.
Bromus setifolius var. pictus (Hook) Skottsb., B. setifolius var. setifolius Presl. and B. setifolius var. brevifolius Ness are three native Patagonian taxa in the section Pnigma Dumort of the genus Bromus L. AFLP and RAPD analysis, in conjunction with genetic distance measurements and statistical techniques, revealed variation within this group and indicated that B. setifolius var. brevifolius was closely related to B. setifolius var. pictus, with both taxa being more distantly related to B. setifolius var. setifolius. Cytogenetic analysis confirmed the chromosomal number of B. setifolius var. pictus (2n = 70) and B. setifolius var. setifolius (2n = 28) and showed for the first time that B. setifolius var. brevifolius had 2n = 70. The combination of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Argentina; Bromus setifolius complex; Cytogenetics; Patagonia; RAPD.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200018

Analysis of genetic population structure in Acacia caven (Leguminosae, Mimosoideae), comparing one exploratory and two Bayesian-model-based methods
Provedor de dados: 74
Autores: Pometti,Carolina L.; Bessega,Cecilia F.; Saidman,Beatriz O.; Vilardi,Juan C.
Bayesian clustering as implemented in STRUCTURE or GENELAND software is widely used to form genetic groups of populations or individuals. On the other hand, in order to satisfy the need for less computer-intensive approaches, multivariate analyses are specifically devoted to extracting information from large datasets. In this paper, we report the use of a dataset of AFLP markers belonging to 15 sampling sites of Acacia caven for studying the genetic structure and comparing the consistency of three methods: STRUCTURE, GENELAND and DAPC. Of these methods, DAPC was the fastest one and showed accuracy in inferring the K number of populations (K = 12 using the find.clusters option and K = 15 with a priori information of populations). GENELAND in turn, provides...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acacia caven; AFLP; GENELAND; DAPC.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100012

Towards an optimal sampling strategy for assessing genetic variation within and among white clover (Trifolium repens L.) cultivars using AFLP
Provedor de dados: 74
Autores: Khanlou,Khosro Mehdi; Vandepitte,Katrien; Asl,Leila Kheibarshekan; Bockstaele,Erik Van.
Cost reduction in plant breeding and conservation programs depends largely on correctly defining the minimal sample size required for the trustworthy assessment of intra- and inter-cultivar genetic variation. White clover, an important pasture legume, was chosen for studying this aspect. In clonal plants, such as the aforementioned, an appropriate sampling scheme eliminates the redundant analysis of identical genotypes. The aim was to define an optimal sampling strategy, i.e., the minimum sample size and appropriate sampling scheme for white clover cultivars, by using AFLP data (283 loci) from three popular types. A grid-based sampling scheme, with an interplant distance of at least 40 cm, was sufficient to avoid any excess in replicates. Simulations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sampling strategy; White clover; AFLP; Genetic diversity parameters.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200015

Somaclonal variation in Asparagus officinalis plants regenerated by organogenesis from long-term callus cultures
Provedor de dados: 74
Autores: Pontaroli,Ana Clara; Camadro,Elsa Lucila.
Somaclonal variation in plants regenerated by organogenesis from long-term cultured calluses of two diploid staminate genotypes of Asparagus officinalis cv. Argenteuil was characterized by plant phenotype, ploidy, meiotic behavior, pollen viability, fruit and seed set, and AFLP profiles. Phenotypic deviations from the donors were detected in foliage color, flower size, and cladode and flower morphology. Ploidy changes were observed in 37.8% of the 37 regenerants studied. Meiotic alterations in 12 out of 21 regenerants included laggards, dicentric bridges, micronuclei, restitution nuclei and polyads. Of the 408 AFLP markers screened in 43 regenerants and the donors, 2.94% showed polymorphism. High pollen viability was observed in the 22 regenerants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Fertility; Plant morphology; Meiotic behavior; Ploidy.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300015

Genetic changes in grapevine genomes after stress induced by in vitro cultivation, thermotherapy and virus infection, as revealed by AFLP
Provedor de dados: 74
Autores: Baránek,Miroslav; Raddová,Jana; Krizan,Bretislav; Pidra,Miroslav.
The Amplification Fragment Length Polymorphism (AFLP) technique was employed to study genetic variations which can be induced in vines by the stress occurring during different aspects of viticulture (in vitro cultivation, in vitro thermotherapy and virus infection). Analysis of AFLP banding patterns, generated by using 15 primer combinations, pointed to negligible genetic variation among plants exposed to individual stress. The average of similarity coefficients between differently stressed plants of the cultivars Müller Thurgau and Riesling were 0.984 and 0.991, respectively, as revealed by AFLP analysis. The low incidence of observed polymorphism demonstrates the high level of genome uniformity in plants reproduced by in vitro micropropagation via nodes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grapevine; Stress; Somaclonal variation; Virus infection; AFLP.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400026

Caracterização molecular da coleção de germoplasma de ora-pro-nóbis (Pereskia spp.) da Embrapa.
Provedor de dados: 14
Autores: MORETZSOHN, M. de C.; SILVA, D. B. da; INGLIS, P. W.; CUSTÓDIO, A. R.; MADEIRA, N. R.; VIEIRA, R. F.
bitstream/item/216009/1/Boletim-Orapronobis-FINAL.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Unconventional food plant; AFLP; Hortaliça não convencional; Variabilidade genética; Genetic variation.
Ano: 2020
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1124941

Génomique de l'adaptation de l'huître creuse du Pacifique, Crassostrea gigas, dans le cadre de son expansion géographique
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).  Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci...
Tipo: Text
Palavras-chave: Pacific oyster; Invasive; AFLP; Genome scan; Crassostrea gigas.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4542.pdf

A first generation genetic linkage map of the European flat oyster Ostrea edulis (L.) based on AFLP and microsatellite markers
Provedor de dados: 5
Autores: Lallias, Delphine; Beaumont, A.r.; Haley, C.s.; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
This study presents the first genetic linkage map for the European flat oyster Ostrea edulis. AFLP and 20 microsatellite markers were genotyped in a three-generation pedigree comprised of 2 grand-parents, 2 parents and 92 progeny. Chi-square goodness of fit tests revealed high segregation distortion, which was significant for 32.8% of markers. Sixteen microsatellites and 235 AFLPs (170 AFLPs type 1:1 and 65 AFLPs type 3:1) were used to build sex-specific linkage maps using CriMap software. The first parental map (P1) consisted of 104 markers grouped in nine linkage groups, spanning 471.2 cM with an average spacing of 4.86 cM. The second parental map (P2) consisted of 117 markers grouped in ten linkage groups (which equals the haploid chromosome number),...
Tipo: Text
Palavras-chave: Microsatellite; AFLP; Ostrea edulis; Flat oyster; Genetic linkage map.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2642.pdf

AFLP-based genetic linkage maps of the blue mussel (Mytilus edulis)
Provedor de dados: 5
Autores: Lallias, Delphine; Lapegue, Sylvie; Hecquet, Celine; Boudry, Pierre; Beaumont, A.
We report the construction of the first genetic linkage map in the blue mussel, Mytilus edulis. AFLP markers were used in 86 full-sib progeny from a controlled pair mating, applying a double pseudo-test cross strategy. Thirty-six primer pairs generated 2354 peaks, of which 791 (33.6%) were polymorphic in the mapping family. Among those, 341 segregated through the female parent, 296 through the male parent (type 1:1) and 154 through both parents (type 3:1). Chi-square goodness-of-fit tests revealed that 71% and 73% of type 1:1 and 3:1 markers respectively segregated according to Mendelian inheritance. Sex-specific linkage maps were built with mapmaker 3.0 software. The female framework map consisted of 121 markers ordered into 14 linkage groups, spanning...
Tipo: Text
Palavras-chave: Mytilus edulis; Genetic linkage map; Blue mussel; AFLP.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2830.pdf

Genetic diversity analysis of isolates belonging to the Photobacterium phosphoreum species group collected from salmon products using AFLP fingerprinting
Provedor de dados: 5
Autores: Jerome, Marc; Mace, Sabrina; Dousset, Xavier; Pot, Bruno; Joffraud, Jean-jacques.
An accurate amplified fragment length polymorphism (AFLP) method, including three primer sets for the selective amplification step, was developed to display the phylogenetic position of Photobacterium isolates collected from salmon products. This method was efficient for discriminating the three species Photobacterium phosphoreum, Photobacterium iliopiscarium and Photobacterium kishitanii, until now indistinctly gathered in the Photobacterium phosphoreum species group known to be strongly responsible for seafood spoilage. The AFLP fingerprints enabled the isolates to be separated into two main clusters that, according to the type strains, were assigned to the two species P. phosphoreum and P. iliopiscarium. P. kishitanii was not found in the collection....
Tipo: Text
Palavras-chave: Photobacterium phosphoreum; AFLP; GyrB-gene; LuxA-gene; Salmon products; Seafood spoilage.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00286/39712/38167.pdf

A first genetic linkage map of the blue mussel Mytilus edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Hecquet, Céline; Boudry, Pierre; Lapegue, Sylvie; Beaumont, Andy.
The blue mussel, Mytilus edulis, is widely distributed in the northern hemisphere, from the White Sea to the Atlantic coast of southern France, and is an economically important species (1 million tons per year, FAO, 2002). At present however, little domestication has been achieved with bivalves, in contrast to agricultural crops, livestock and horticultural species like tomatoes, where selection and production is entirely controlled by man. For bivalves, despite their economic importance, no lines with selected characteristics have been taken to the production scale. There is therefore a need to make up for lost time. Our interest in genetic mapping sprang from some results showing a genetic component to some economically important traits like growth and...
Tipo: Text
Palavras-chave: QTLs; AFLP; Genetic; Mussel; Mytilus edulis; Genetic linkage map.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3465.pdf

Genetic linkage mapping in the blue mussel Mytilus edulis and the European flat oyster Ostrea edulis, and the search for Quantitative Trait Loci of resistance to a disease in O. edulis.
Provedor de dados: 5
Autores: Lallias, Delphine.
Genetic linkage maps consist of ordering molecular markers across the genome and require a high number of markers for a good coverage of the genome. Such maps represent a framework which enables the identification and localisation of Quantitative Trait Loci (QTLs) for traits of interest, such as growth or disease resistance, with the final aim of achieving genetic improvement through marker-assisted selection (MAS). Data on bivalves are scarce. No genetic map has yet been constructed in any mussel or flat oyster species. We report construction of a preliminary genetic linkage map in the blue mussel, Mytilus edulis (n=14). Amplified Fragment Length Polymorphism (AFLP) markers were used in a mapping family containing 86 full-sibs issued from a controlled...
Tipo: Text
Palavras-chave: Microsatellite; AFLP; Mussel; Flat oyster; QTL; Genetic mapping; Microsatellite; AFLP; Moule; Huître plate; QTL; Cartographie génétique.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/these-2603.pdf

Scanning the genome of the Pacific cupped oyster, Crassostrea gigas, for adaptative differentiation during geographic range expansion
Provedor de dados: 5
Autores: Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci...
Tipo: Text
Palavras-chave: Invasive; AFLP; Genome scan; Crassotrea gigas; Pacific oyster.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4805.pdf

Genetic diversity and adaptability of two species of Mugilidae (Teleostei : Perciformes) of the Po river delta coastal lagoons
Provedor de dados: 5
Autores: Papa, R; Nonnis Marzano, F; Rossi, V; Gandolfi, G.
Molecular polymorphisms (allozyme and amplified fragment length polymorphisms (AFLP)) were investigated in the mullet specie's Liza ramada and Liza saliens to determine levels of genetic variability and assess possible correlations between the population genetic structure and the specie's adaptability to shallow water environments. The thinlipped (L. ramada) and the sharpnose (L saliens) mullets are diadromous species-that enter coastal lagoons during early life stages to complete growth previous to sexual maturation. Bi-monthly samples (n = 30-50) were collected in the period 2000-2002 in different sites of the Sacca di Goro (Po river delta) and a nearby aquaculture lagoon (Valle Bertuzzi). Biochemical analyses were carried out by means of starch gel...
Tipo: Text
Palavras-chave: AFLP; Systèmes enzymatiques; Electrophorèse capillaire; Liza spp; Polymorphisme moléculaire; AFLP; Allozyme; Capillary electrophoresis; Liza spp.; Molecular polymorphisms.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00322/43292/43027.pdf

A multivariate approach to explore the genetic variability in the F2 segregating population of a tomato second cycle hybrid
Provedor de dados: 46
Autores: Cabodevila,V.G; Picardi,L.A; Pratta,G.R.
Segregating progeny from the tomato Second Cycle Hybrids (SCH) that were obtained from crossing RIL (Recombinant Inbred Lines) allows the detection of new genetic combinations that could increase genetic variability in F2 populations. The objectives of the present study were to evaluate eleven tomato quality traits in a segregating F2 population obtained from a SCH and, then, to characterize the molecular diversity by six AFLP (Amplified Fragment Length Polymorphism) primer combinations. Different multivariate analyses were used to assess the degree of concordance among these two approaches to detect genetic variability. Sixty-nine F2 plants were obtained by selfing the SCH (ToUNR18xToUNR1). The parental RIL were derived from an interspecific cross between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant breeding; Plant genetic resources; Fruit quality; AFLP; Multivariate analysis.
Ano: 2017
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332017000100001

On the identity of broad-shelled mussels (Mollusca, Bivalvia, Mytilus) from the Dutch delta region
Provedor de dados: 16
Autores: Groenenberg, D.S.J.; Wesselingh, F.P.; Rajagopal, S.; Jansen, J.M.; Bos, M.M.; Velde, G. van der; Gittenberger, E.; Hoeksema, B.W.; Raad, H.; Hummel, H.
Late Quaternary (Eemian) deposits of the Netherlands contain shells that resemble those of living Mytilus galloprovincialis. Similar broad-shelled mytilids also occur in estuaries of the southwestern Netherlands together with slender individuals typical of M. edulis. We sampled living mussels along a depth gradient in the Oosterschelde to a) investigate whether a relation exists between shell shape and depth, b) test if the broadshelled specimens might represent M. galloprovincialis (or a hybrid with M. edulis) and c) assess by inference if the Quaternary specimens might be attributed to M. galloprovincialis as well. In order to do so, we compared genetic (length polymorphism of Me 15/16, COIII sequences and AFLPs) and shellmorphological characteristics...
Tipo: Article / Letter to the editor
Palavras-chave: AFLP; COIII; Me 15/16; Mytilus; Shell morphology; The Netherlands; 42.73.
Ano: 2011
URL: http://www.repository.naturalis.nl/record/376595

Análise da estrutura e diversidade molecular de populações de Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) associadas às culturas de milho e arroz no Rio Grande do Sul
Provedor de dados: 82
Autores: Busato,Gustavo R.; Grützmacher,Anderson D.; Oliveira,Antônio C. de; Vieira,Eduardo A.; Zimmer,Paulo D.; Kopp,Maurício M.; Bandeira,Juliana de M.; Magalhães,Taís R.
O objetivo do trabalho foi analisar a estrutura e a diversidade molecular de quatro populações de Spodoptera frugiperda (J.E. Smith) associadas às culturas de milho e arroz no Rio Grande do Sul. Foram coletadas lagartas de quatro populações em áreas isoladas (distanciadas em mais de 300 km) nos municípios de Santa Rosa (milho) e Uruguaiana (arroz irrigado) e em áreas adjacentes, no município de Pelotas (milho e arroz irrigado). A análise de 40 lagartas (10 de cada população), com cinco combinações de oligonucleotídios iniciadores geraram o total de 241 locos, dentre os quais 229 (95%) foram polimórficos. O dendrograma gerado pelo método de agrupamento UPGMA, baseado na matriz de similaridade de Dice, mostrou sobreposições entre as lagartas das populações...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Lagarta-do-cartucho; Lagarta-da-folha; AFLP; Biótipo.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000600008

Correlação da heterose de híbridos de milho com divergência genética entre linhagens
Provedor de dados: 86
Autores: Guimarães,Paula de Souza; Paterniani,Maria Elisa Ayres Guidetti Zagatto; Lüders,Reginaldo Roberto; Souza,Anete Pereira de; Laborda,Prianda Rios; Oliveira,Karine Miranda.
O objetivo deste trabalho foi avaliar a produtividade de híbridos simples de milho obtidos de cruzamentos dialélicos entre linhagens divergentes, estimar a capacidade de combinação das linhagens e confirmar se a divergência genética entre as linhagens, obtida por marcadores moleculares, é correlacionada com a heterose dos híbridos simples no campo. Trinta e seis híbridos resultantes do dialelo parcial e as 12 linhagens parentais foram avaliadas em Campinas em blocos ao acaso, com três repetições e duas testemunhas. A capacidade de combinação das linhagens foi estimada de acordo com o modelo 4 de Griffing. Estimaram-se a correlação matricial, mediante a estatística de Mantel, entre heterose, produtividade e capacidade específica de combinação com...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Marcadores moleculares; Dialelo parcial; Capacidade de combinação; AFLP; SSR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000600007

Morphophysiogenetic characterization of wild poinsettia biotypes with low resistance to glyphosate
Provedor de dados: 86
Autores: Agostinetto,Dirceu; Ulguim,André da Rosa; Vargas,Leandro; Silva,Jessica Dias Gomes da; Langaro,Ana Claudia.
Abstract: The objective of this work was to compare wild poinsettia (Euphorbia heterophylla) biotypes as to their morphological characteristics, as well as to determine their genetic similarity in the state of Rio Grande do Sul, Brazil, and to confront the light compensation point of susceptible biotypes and of biotypes with low-resistance level to glyphosate. Besides the morphology analysis, the study included the genetic characterization, by AFLP, of susceptible (GR50= 58.65 g ha-1 a.e.) biotypes and of biotypes with low resistance (GR50= 310.36 g ha-1 a.e.) to glyphosate. The competitive ability of the biotypes was inferred according to their light compensation point. The observed morphological characteristics do not allow to differentiate biotypes as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euphorbia heterophylla; AFLP; Competitive ability; EPSPs inhibitor herbicide; Light compensation point; Resistance to herbicide.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001100987

Potato cultivar identification using molecular markers
Provedor de dados: 86
Autores: Rosa,Patricia Marchezi; Campos,Tatiana de; Sousa,Adna Cristina Barbosa de; Sforça,Danilo Augusto; Torres,Gisele Abigail Montan; Souza,Anete Pereira de.
The objective of this work was to evaluate a set of microsatellite markers for varietal identification and characterization of the most widespread potato cultivars in Brazil. The DNA from 14 potato cultivars was genotyped using microsatellite markers and the alleles were scored in silver-stained polyacrylamide gel. Twenty-four microsatellite markers were evaluated, and only one locus was monomorphic. Based on band patterns, a set of two microsatellites that were able to identify and differentiate all examined cultivars was obtained.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum; AFLP; Genetic certification; Varietal protection; Variety identification; RAPD.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000100015

Agrupamiento de genotipos de nopal (Opuntia spp.) de México por medio de la técnica de AFLPs y características del fruto
Provedor de dados: 85
Autores: Espinoza Sánchez,EA; Silos Espino,H; Flores Benitez,S; Valera Montero,LL; Rodríguez Salazar,E; Gallegos Vázquez,C; Guevara Lara,F; González Chavira,M; Guzmán Maldonado,HS.
Para contribuir al conocimiento de la diversidad genética del nopal se analizaron 85 genotipos representativos de Opuntia (silvestres y cultivados) por medio de la técnica de AFLPs y características del fruto. El análisis de AFLPs con los iniciadores Mse ICAC/Eco RI-AAG generaron más fragmentos polimórficos (57,5 en promedio) por genotipo; se obtuvo un dendrograma que no mostró una clara relación de acuerdo a la taxonomía conocida. En complemento al perfil genético, y para permitir una referencia morfológica o química directa, agrupamos a los genotipos de acuerdo al contenido de °Brix de sus frutos, debido a que esta es una característica primordial para su identificación visual y aprovechamiento de consumo directo. Se encontró que los genotipos que...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caracterización Molecular; Opuntia; AFLP; Dendrograma; Morfología del fruto.
Ano: 2014
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572014000200010

AFLP polymorphism in restored provenances of Ceiba aesculifolia within an urban heat island
Provedor de dados: 85
Autores: Olvera-Mendoza,EI; Lara-Cabrera,SI; Sáenz-Romero,C; Lindig-Cisneros,R.
To quantify the effect on genetic diversity of restoring tree populations in an area under the influence of an urban heat island, we evaluated the genetic diversity of 72 Ceiba aesculifolia individuals, from a restoration experiment established from 2170 to 2260 m a.s.l. Reintroduced individuals were compared with the provenance from which the seeds were obtained, and two external provenances, for a total of 123 individuals. Samples were analyzed with three AFLP primer combinations. Polymorphisms of 38.4 to 62.5% were obtained. Genetic diversity estimated with the Simpson index ranged from 0.14 to 0.2. The provenances in the restoration site had higher diversity than the provenance that originated the seeds. Groups formed with provenances with similar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; AFLP; Restoration ecology; Ceiba aesculifolia; Protected Natural Area.
Ano: 2016
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200001

Estimación de la diversidad genética del Nopal, usando marcadores moleculares tipo AFLP
Provedor de dados: 85
Autores: García-Zambrano,EA; Zavala-García,F; Gutiérrez-Diez,A; Ojeda-Zacarías,MC; Cerda- Hurtado,I.
El presente estudio tiene por objetivo aplicar la técnica de los marcadores moleculares tipo AFLP para la estimación de la diversidad genética en nopal dentro del Banco de Germoplasma de la FAUANL, donde se han reportado 12 accesiones como posibles duplicados por medio de marcadores moleculares tipo RAPD. El método utilizado para la extracción de ADN fue el del ruptor celular. El ADN fue digerido y ligado, para posteriormente realizar una preamplificación y después la amplificación selectiva. Los fragmentos amplificados fueron luego separados y analizados. Se concluyó que ninguna de las accesiones estaba duplicada. Esto se debió a que en el dendograma generado al usar el método de UPGMA se formaron 8 grupos. Dos de estos grupos lo integraron 3 accesiones...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marcadores moleculares; AFLP; Accesiones.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572009000200006

Variability and Genetic Structure in Populations of Arrabidaea bilabiata (Sprague) Sandwith in the Amazonas State
Provedor de dados: 87
Autores: Souza,L.S.A.; Silva,J.F.; Lopes,M.T.G.; Bentes,J.L.S.; Valente,M.S.F.; Costa Neto,P.Q.
Weeds in pastures can intoxicate animals, and Arrabidaea bilabiata is the most important species for herbivores in floodplain areas in the Amazon Basin. Genetic diversity studies in natural populations may contribute to the better understanding of the range of toxicity and the genetic variability organization in this species. The objective of this study was to assess the variability and genetic structure in six populations of A. bilabiata sampled in floodplain areas in three municipalities of the Amazonas State, from the AFLP markers analysis. AFLP markers were efficient to characterize the genetic variability of the 65 individuals analyzed. From four combinations of oligonucleotides, a total of 309 AFLP fragments was obtained, where 304 (98.38%) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arrabidaea bilabiata; Pasture weeds; Poisonous plants; AFLP; Genetic diversity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000200213

Genetic diversity among proso millet (Panicum miliaceum) biotypes assessed by AFLP technique
Provedor de dados: 87
Autores: Karam,D.; Westra,P.; Nissen,S.J.; Ward,S.M.; Figueiredo,J.E.F.
The Amplified Fragment Length Polymorphism (AFLP) technique was used to access genetic diversity between three domestic and nine wild proso millet biotypes from the United States and Canada. Eight primer combinations detected 39 polymorphic DNA fragments, with the genetic distance estimates among biotypes ranging from 0.02 to 0.04. Colorado-Weld County black seeded and Wyoming-Platte County were the most distinct biotypes according to the dissimilarity level. A UPGMA cluster analysis revealed two distinct groups of proso millet without any geographic association. Six weed biotypes exhibiting some characters of cultivated plants were grouped together with domesticated biotypes of proso millet while the three typical wild phenotypes were clearly clustered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proso millet; AFLP; Taxonomic relationship; Genetic diversity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582004000200001

Assessment of silver-stained AFLP markers for studying DNA polymorphism in proso millet (Panicum miliaceum L.)
Provedor de dados: 88
Autores: Karam,Décio; Westra,Philip; Niessen,Scott J.; Ward,Sarah M.; Figueiredo,José Edson Fontes.
Proso millet (Panicum miliaceum L.) is a serious weed in North America. A high number of wild proso millet biotypes are known but the genetic basis of its phenotypic variation is poorly understood. In the present study, a non-radioactive silver staining method for PCR-Amplified Fragment Length Polymorphism (AFLP) was evaluated for studying genetic polymorphism in American proso millet biotypes. Twelve biotypes and eight primer combinations with two/three and three/three selective nucleotides were used. Pair of primers with two/three selective nucleotides produced the highest number of amplified DNA fragments, while pair of primers with three/three selective nucleotides were more effective for revealing more polymorphic DNA fragments. The two better primer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Genetic diversity; Molecular markers; Proso-millet.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042006000400011

Inter-relações genéticas entre espécies do gênero Spondias com base em marcadores AFLP
Provedor de dados: 94
Autores: Santos,Carlos Antônio Fernandes; Oliveira,Viseldo Ribeiro de.
O objetivo deste trabalho foi construir um fenograma de trinta indivíduos de seis espécies de Spondias, amostradas em três diferentes locais, baseado em 120 marcadores AFLP das combinações de primers EcoR1/Mse1 para esclarecer as inter-relações entre as espécies, subsidiar a exploração das espécies como porta-enxerto e fornecer informações genômicas dessas espécies. O fenograma UPGMA do coeficiente de Jaccard apresentou boa definição, com valor co-fenético de 0,925 e localização externa de dois "outgroups" das espécies Mangifera indica e Schinopsis brasiliensis da família Anacardeaceae. Todos os indivíduos de S. cytherea, S. tuberosa e S. purpurea foram agrupados dentro de cada espécie, independentemente do local de amostragem, enquanto quatro dos seis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spondias tuberosa; Fenograma; Marcadores moleculares; DNA; AFLP; Genética.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452008000300028

Detecção de polimorfismo em porta-enxertos para citros
Provedor de dados: 94
Autores: Andrade,Renata Aparecida de; Martins,Antonio Baldo Geraldo; Lemos,Eliana Gertrudes de Macedo; Luz,Francisco Joaci de Freitas; Silva,Marco Túlio Habib.
Objetivando a verificação da existência de plântulas originadas de embrião zigótico em viveiros comerciais de mudas cítricas, realizou-se o presente trabalho, utilizando a técnica de fAFLP e quatro espécies de porta-enxertos para citros. Verificou-se uma base genética estreita entre as espécies testadas, além de grande variabilidade entre os materiais, independentemente do viveiro em que foram coletados, o que permite concluir que a seleção visual, comumente realizada nos viveiros, é ineficiente.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Citrus limonia; Citrus reshni; Citrus sunki; Citrus paradise x Poncirus trifoliata.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452007000200030

Diversidad genética de Trichoderma spp. en Venezuela, determinada mediante análisis combinado ITS-AFLP
Provedor de dados: 100
Autores: Sosa,Daynet; Pérez Martínez,Simón; Molina,Sandy; Demey,Jhonny; Gómez,Kelly; Domínguez,Diamarys; Istúriz,María; Rumbos,Raisa; Parra,Dercy.
Se estudió la diversidad genética entre 68 aislados de Trichoderma procedentes de diferentes cultivos, sustratos y localidades, mediante los análisis ITS-AFLP. Se secuenció la región ITS1-5.8S-ITS2 del ADN ribosomal amplificada con los cebadores ITS1 e ITS4 y en el caso de los AFLP se utilizaron cuatro combinaciones de oligonucleótidos. Las relaciones genéticas entre los aislados se analizaron mediante el uso combinado del Análisis de Coordenadas Principales, el Análisis de Conglomerados y el ajuste de un Biplot Logístico Externo sobre datos de disimilitud, utilizando los coeficientes de Jaccard, Emparejamiento simple, Dice y Rogers y Tanimoto. Se identificaron nueve especies y las más abundantes fueron Hypocrea lixii (anamorfoTrichoderma harzianum) y T....
Tipo: Journal article
Palavras-chave: Trichoderma; ITS; AFLP; Polimorfismo; Control biológico.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000100005

Morphological and molecular characterization of Puccinia kuehnii, the causal agent of sugarcane orange rust, in Cub
Provedor de dados: 63
Autores: Perera,María Francisca; Bertani,Romina Priscila; Arias,Marta Eugenia; Hechavarría,María de la Luz La O; Navarro,María de los Ángeles Zardón; Debes,Mario Alberto; Luque,Ana Catalina; Cuenya,María Inés; Rojas,Ricardo Acevedo; Castagnaro,Atilio Pedro.
ABSTRACT Sugarcane orange rust caused by Puccinia kuehnii has recently become an important disease in sugarcane crops and its spread is causing great concern to growers. In this study, we analyzed spores from symptomatic orange rust sugarcane leaves collected in multiple locations in Cuba in a 4-year-period in order to characterize morphological traits of P. kuehnii, establish an adequate molecular technique to characterize it, and determine its infection court in sugarcane. Orange rust caused by P. kuehnii was confirmed by polymerase chain reaction (PCR) and morphological characterization. AFLP markers detected high diversity in P. kuenhnii samples. Sequencing of rDNA regions, as expected, did not reveal differences and SSR markers designed for P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; RDNA; SSR; Infection courts.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201200

ความหลากหลายทางพันธุกรรมและชีวชนิดของแมลงบั่วในประเทศไทย
Provedor de dados: 106
Autores: Jirapong Jairin; Kanlaya Sansen; Sa-nguan Teangdeerith; Phikul Leelakud; Rungnapa Kawichai; Wantana Siratanasak; Nalinee Chiengwattana.
Amplified fragment length polymorphism (AFLP) and biotype-specific markers were used for identification of genetic diversity and biotypes of rice gall midge, Orseolia oryzae in Thailand. Pupae and adults of the gall midges were collected from 19 field locations of 12 provinces during 2006 - 2007. Total of 242 polymorphic AFLP bands were generated from 12 selective primer pairs. Cluster analysis, performed by the UPGMA, separated the gall midge populations into four distinct groups. To identify the biotype, five specific-biotype markers were used to characterize the collected gall midges. Two biotypes of the gall midges, biotype 2 and 5, were identified from all populations used in this research. The results showed that the genetic diversity was not...
Tipo: Collection
Palavras-chave: Orseolia oryzae; Rice gall midge; Biotype; Genetic diversity; AFLP; ข้าว; แมลงบั่ว; การจำแนกชีวชนิด; ความหลากหลายทางพันธุกรรม; เอเอฟแอลพี; โมเลกุลเครื่องหมาย; ประเทศไทย.
Ano: 2009
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5092

Separation of the genera in the subtribe Cassiinae (Leguminosae: Caesalpinioidae) using molecular markers
Provedor de dados: 39
Autores: Acharya,Laxmikanta; Mukherjee,Arup Kumar; Panda,Pratap Chandra.
Random amplified polymorphic DNA (RAPD), Inter simple sequence repeat (ISSR) and Amplified fragment length polymorphism (AFLP) markers were used to verify the segregation of the genus Cassia L. senso lato into three distinct genera namely Chamaecrista Moench., Senna P. Mill. and Cassia L. sensostricto Eighteen representatives of the three taxa were characterized using the molecular markers. 25 RAPD, six ISSR primers and six AFLP primer combinations resulted in the amplification of 612, 115 and 622 bands (loci) respectively. Most of the loci are found to be polymorphic, showing high degrees of genetic diversity among the different taxa studied. The dendrogram constructed on the basis of the RAPD, ISSR and AFLP data using SHAN clustering, divided Cassia L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Cassia; ISSR; Molecular phylogeny; RAPD.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062011000100026

Identificación de Cultivares y Líneas de Mejoramiento de Arroz de Chile Mediante Amplificación de Fragmentos Polimórficos (AFLP)
Provedor de dados: 113
Autores: Aguirre,Carlos; Alvarado,Roberto; Hinrichsen,Patricio.
Doce cultivares y líneas de arroz (Oryza sativa L.) desarrollados por el programa de fitomejoramiento del Instituto de Investigaciones Agropecuarias (INIA), Centro Regional de Investigación Quilamapu, fueron caracterizados genéticamente mediante amplificación de fragmentos polimórficos (AFLP). De 21 combinaciones ensayadas, sólo 16 fueron informativas, generando un total de 667 amplicones, 94 de ellos polimórficos (14,4%), con un rango entre 9 y 33% de polimorfismo por combinación. Esto indica que el material manejado por el programa de INIA presenta un bajo nivel de diversidad genética comparado tanto con germoplasma silvestre de la especie como con aquel usado por otros programas de fitomejoramiento de la especie. Los amplicones polimórficos detectados...
Tipo: Journal article
Palavras-chave: Diversidad genética; Fingerprinting; AFLP; Pedigrí; Cultivares chilenos de arroz.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072005000400002

Genetic characterization of pomegranate (Punica granatum L.) genotypes by AFLP markers
Provedor de dados: 48
Autores: Ercisli,Sezai; Kafkas,Ebru; Orhan,Emine; Kafkas,Salih; Dogan,Yildiz; Esitken,Ahmet.
The Coruh Valley, located in Northeastern Turkey, is one of the most important centers of diversity in pomegranate in Turkey. In this study, we attempted to characterize 19 promising pomegranate genotypes originating from the Coruh Valley in using fluorescent dye AFLP markers and capillary electrophoresis. Four AFLP primer combinations were used, generating a total of 297 fragments, 213 of which were polymorphic (73.0%). Resolving powers of the AFLP primers ranged from 0.700 to 1.018, with a total of 3.440, while polymorphism information contents ranged from 0.707 to 0.837 with an average of 0.764. UPGMA clustering of the genotypes showed two major groups. Most of the fruit characteristics of the genotypes within the same group were variable. Therefore,...
Tipo: Journal article
Palavras-chave: Genetic diversity; AFLP; Pomegranate.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400005

Morphological and molecular characterization of common bean landraces cultivated in the semi-arid Mexican high plateau
Provedor de dados: 51
Autores: Gill Langarica,Homar Rene; Rosales Serna,Rigoberto; Hernandez Delgado,Sanjuana; Mayek Perez,Netzahualcoyotl.
The objective of the current research was to characterize the common bean in agricultural fields planted with common bean landraces from the semi-arid Mexican high plateau using both morphological and amplified fragment length polymorphism (AFLPs) data. The morphological traits were discriminating and exhibited the clustering of 150 accessions based on the geographic origin and seed coat color. AFLP primer combinations exhibited a polymorphic range between 0.292 (E-AGG + M-ACT) and 0.375 (E-ACA + M-AGA). The frequency and distribution of the polymorphic fragments allowed the detection of a larger number of rare fragments in accessions 121 and 111 (Flor de Mayo and black common beans, respectively). The analysis of genetic relationships, analysis of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Genetic variation; Landraces; Phaseolus vulgaris L.
Ano: 2014
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722014000400010

No differences ingenetic diversity of Cotoneaster franchetii (Rosaceae) shrubs betweennative and non-native ranges
Provedor de dados: 51
Autores: Lett,Irene; Hensen,Isabell; Hirsch,Heidi; Renison,Daniel.
It is commonly assumed that plants have more genetic diversity in their native range than in areas where they have been introduced due to founder effects. However, few studies have proven this assumption and included the comparison between non-native and native ranges. We analyzed AFLP fingerprint patterns of 149 individuals from five native (China) and five non-native (Argentina) populations of Cotoneaster franchetii, a shrub which successfully invades different habitats and forms extensive monospecific stands. We compared genetic diversity estimates and assessed genetic differentiation among populations by inspecting FST values and conducting a PCoA, an AMOVA and a Mantel test. No evidence was found for reduced genetic diversity in non-native populations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Argentina; Biological invasion; China; Genetic differentiation; Multiple introductions.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722015000300010

Genetic diversity in a natural population of the halophytic legume Prosopis strombulifera revealed by AFLP fingerprinting
Provedor de dados: 51
Autores: Llanes,Analia; Bonercarrere,Victoria; Capdevielle,Fabian; Vidal,Sabina; Luna,Virginia.
Prosopis strombulifera (Lam.) Benth. is a spiny shrub with the maximum tolerance limits reported for halophytic plants. This species is frequently found in the salinized areas in south-western of Córdoba and San Luis provinces, Argentina. Little is known about the genetic diversity within this species in a native population. Genetic diversity in 60 plants of P. strombulifera in south-western San Luis was investigated using AFLP analysis. Polymorphism was found among the samples tested. Four combinations of primers led to the identification of an average of 250 polymorphic bands and the data were used for cluster analysis. P. strombulifera genotypes are clearly separated in subclusters and reflect the diversity within the collection area. This study is a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prosopis strombulifera; AFLP; Genetic diversity; Polymorphism.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722011000200010

Evaluación del germoplasma de Ilex paraguariensis e Ilex dumosa (Aquifoliaceae)
Provedor de dados: 51
Autores: Gottlieb,Alexandra Marina; Giberti,Gustavo Carlos; Poggio,Lidia.
Ilex paraguariensis e I. dumosa constituyen dos especies de gran relevancia económica para nuestro país. Estas comparten muchas características de su biología, aunque se diferencian en composición fitoquímica y resistencia a factores bióticos y abióticos. El objetivo del presente trabajo es evaluar la estructura de la diversidad genética de plantas mantenidas en el Banco de Germoplasma de Yerba Mate y Té de la Estación Experimental del INTA en Cerro Azul (Misiones) y de materiales comerciales de yerba mate, mediante el re-análisis de datos AFLP, así como caracterizar las secuencias nucleotídicas de bandas potencialmente diagnósticas de especie. En I. dumosa se detectó, respecto de I. paraguariensis, un nivel de variación molecular dos veces mayor, aunque...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Yerba mate; Yerba señorita; Germoplasma; Diferenciación genética.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722011000100008

Comparação entre medidas de distância genealógica, morfológica e molecular em aveia em experimentos com e sem a aplicação de fungicida
Provedor de dados: 53
Autores: Vieira,Eduardo Alano; Carvalho,Fernando Irajá Félix de; Oliveira,Antônio Costa de; Benin,Giovani; Zimmer,Paulo Dejalma; Silva,José Antônio Gonzalez da; Martins,Andreza Figueirola; Bertan,Ivandro; Silva,Giovani Olegário da; Schmidt,Douglas André Mallman.
A estimativa da divergência genética entre cultivares pode ser útil em um programa de melhoramento para o direcionamento de hibridações e o conhecimento do germoplasma disponível. O objetivo do trabalho foi estimar a dissimilaridade genética entre genótipos de aveia (Avena sativa), por meio de marcadores AFLP e do coeficiente de parentesco, e correlacionar essas estimativas entre si e com a estimativa da dissimilaridade genética a partir de caracteres agronômicos em experimentos com e sem a aplicação de fungicida. Onze cultivares de aveia foram utilizadas na estimativa da distância genética a partir de marcadores AFLP. Desses 11 genótipos, nove foram avaliados no campo em experimento com e sem fungicida, para a estimativa da divergência genética a partir...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aveia-branca; Divergência genética; AFLP; Coeficiente de parentesco; Distância fenotípica.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052005000100006

Capacidade combinatória, divergência genética entre linhagens de milho e correlação com heterose
Provedor de dados: 53
Autores: Paterniani,Maria Elisa Ayres Guidetti Zagatto; Guimarães,Paula de Souza; Lüders,Reginaldo Roberto; Gallo,Paulo Boller; Souza,Anete Pereira de; Laborda,Prianda Rios; Oliveira,Karine Miranda.
Objetivou-se avaliar o desempenho de híbridos simples de milho (Zea mays L.), obtidos de cruzamentos dialélicos entre linhagens divergentes, estimar a capacidade de combinação das linhagens e verificar se a divergência genética entre as linhagens, obtida por marcadores moleculares, é correlacionada com a heterose dos híbridos simples no campo. Trinta e seis híbridos resultantes de um dialelo parcial foram avaliados em Campinas e em Mococa e as 12 linhagens parentais somente em Campinas, a fim de se calcular a heterose dos híbridos. O delineamento experimental empregado foi o de blocos ao acaso, com três repetições e 2 testemunhas comerciais. Avaliaram-se os caracteres: altura da planta (AP), altura da espiga (AE) e massa de grãos (MG). Análises de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Dialelo parcial; Produtividade; AFLP; SSR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052008000300012

Divergência genética entre genótipos de alface por meio de marcadores AFLP
Provedor de dados: 53
Autores: Sousa,Cristina Soares de; Bonetti,Ana Maria; Goulart Filho,Luiz Ricardo; Machado,Jane Rodrigues de Assis; Londe,Luciana Nogueira; Baffi,Milla Alves; Ramos,Renato Graciliano; Vieira,Carlos Ueira; Kerr,Warwick Estevam.
Considerando a restrita diversidade de espécies disponíveis para nutrir a carência de vitaminas no Brasil, Kerr e colaboradores, desde 1981, vêm desenvolvendo pesquisas para melhoramento genético de hortaliças ricas em vitamina A. Dentre elas, obtiveram uma cultivar de alface, denominada Uberlândia 10.000 com 10.200 UI de vitamina A em 100 gramas de folha fresca. Este trabalho objetivou comparar o grau de divergência genética entre a cultivar Uberlândia 10.000 e seus parentais para avaliar a eficiência da seleção utilizada, por meio da técnica AFLP. Foram utilizados os seguintes genótipos de alface: Maioba, Salad Bowl-Mimosa, Moreninha-de-Uberlândia, Vitória de Santo Antão, Uberlândia 10.000 lisa 8.ª e 9.ª geração e Uberlândia 10.000 crespa 8.ª e 9.ª...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Divergência genética; AFLP; Alface.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052007000100002

Genetic Diversity among Flue-cured Tobacco Cultivars Based on RAPD and AFLP Markers
Provedor de dados: 52
Autores: Zhang,Han Yao; Liu,Xiao Zhen; He,Chuan Sheng; Yang,Yu Ming.
The aim of this work was to study the genetic diversity among flue-cured tobacco cultivars. RAPD and AFLP analyses were used to assess the genetic similarity among selected accessions of flue-cured tobacco. Seventy eight RAPD and 154 AFLP polymorphic bands were obtained and used to assess the genetic diversity among 28 tobacco accessions. The cultivar relationships were estimated through the cluster analysis (UPGMA) based on RAPD data and AFLP data. The accessions were grouped into three major clusters and these shared common ancestry clustered together.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flue-cured tobacco; RAPD; AFLP; Genetic diversity; DNA polymorphism.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600003

Use of single-enzyme amplified fragment length polymorphism for typing Clostridium perfringens isolated from diarrheic piglets
Provedor de dados: 58
Autores: Shinya,Luciane Tieko; Baccaro,Maria Regina; Moreno,Andrea Micke.
Clostridium perfringens is an important pathogen in human and veterinary medicine. In swine, the agent is responsible for necrotic enteritis and enterotoxemia characterized by diarrhea, weight loss, delayed development and, in some cases, death. In the present study amplified fragment length polymorphism analyses (AFLP) was used to characterize 54 C. perfringens strains isolated from swine presenting diarrhea. Analysis of the results showed 29 distinct profiles with discriminatory index equal to 0.97. Partial correlation between the origin of the isolates and groups was drawn, and correlation was possible in only 18.5% of the samples. Characterization of the strains in biotypes (A, B, C, D and E), production of beta-2 toxin and enterotoxin were performed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Diarrhea; Piglets; Clostridium perfringens.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300034

Genetic Characterization of Old Grapevines collected in Oases of the Atacama Desert
Provedor de dados: 117
Autores: Poblete,Ingrid; Pinto,Manuel; de Andrés,María Teresa; Hinrichsen,Patricio.
Old grapevine (Vitis vinifera L.) accessions are a source of genes that could be rescued for use per se or in modern breeding programs. The first step in this rescuing is collecting and characterizing the germplasm from a particular region. This study presents the genetic characterization of 21 grapevine accessions collected from the Atacama Desert in the far North of Chile. Characterization was based on 12 microsatellites (Simple Sequence Repeats, or SSRs) supplemented with Amplified Fragment Length Polymorphic (AFLP) markers. Most of the collected accessions produced red berries and shared the genetic characteristics of the cv. País, an old genotype found throughout America. However, among those red-berried accessions, one showed a severe abortive...
Tipo: Journal article
Palavras-chave: SSR; AFLP; Atacama Desert; Germplasm; ‘Listán Prieto’.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300020

Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods
Provedor de dados: 65
Autores: Mega,Nicolás Oliveira; Revers,Luís Fernando.
Here, it is presented a rapid and efficient method to obtain good quality DNA from small samples of arthropod tissues generating low quantities of hazardous wastes. This new method was compared with another homemade protocol using phenol and other two commercial kits. The quality of DNA obtained was checked by spectrophotometer and evaluated by an AFLP assay. Low shearing DNA was obtained from all samples and the best readings were observed to DNA recollected with the new method. The AFLP assay indicated that DNA obtained with all methods were suitable for use in molecular biology techniques sensitive to contaminants. However, homemade protocols were more efficient in recollect DNA than commercial kits, without lose any quality of samples. Also, they were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arthropod DNA; High quality DNA; Molecular biology; AFLP.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000900012

Variabilidade genética interespecífica em formigas cortadeiras do gênero Acromyrmex que ocorrem no Estado do Rio Grande do Sul
Provedor de dados: 65
Autores: Grutzmacher,Douglas Daniel; Loeck,Alci Enimar; Oliveira,Antônio Costa; Zimmer,Paulo Dejalma; Malone,Gaspar.
São poucos os taxonomistas que atuam na identificação de formigas cortadeiras. Os métodos morfométricos utilizados nem sempre permitem uma identificação confiável, em função do acentuado polimorfismo dentro do mesmo formigueiro. O objetivo do trabalho foi testar a possibilidade do uso da ferramenta da biologia molecular para auxiliar na identificação das espécies de formigas cortadeiras do gênero Acromyrmex que ocorrem no Estado do Rio Grande do Sul. O estudo foi realizado com seis espécies de formigas do gênero Acromyrmex coletadas em quatro regiões do RS. Utilizaram-se as técnicas RAPD e AFLP, testando-se inicialmente 50 primers, dos quais apenas 13 foram selecionados por terem amplificado com sucesso fragmentos de todas as espécies estudadas. Embora os...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quenquéns; RAPD; AFLP; Primers.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000400001

Pareamento de fungos cultivados por diferentes espécies de formigas cortadeiras no Rio Grande do Sul
Provedor de dados: 65
Autores: Borba,Regina da Silva; Loeck,Alci Enimar; Branco,Juliana Severo Castelo; Bonow,Juliane; Oliveira,Antônio Costa de.
Este trabalho teve por objetivo estudar a possível ocorrência de heterocariose por meio do pareamento de fungos provenientes de diferentes espécies de formigas cortadeiras coletados em diferentes regiões do Estado do Rio Grande do Sul, utilizando a técnica AFLP. Dez grupos de pareamentos foram realizados, sendo que em dois não ocorreu a fusão das colônias. Após ocorrerem os pareamentos, foram coletados discos de micélio da área de intersecção das colônias e das áreas extremas de pareamento, para a realização da extração do DNA e padronização. Para as reações de PCR, utilizaram-se 21 combinações de primers AFLP, dos quais foram selecionados três que apresentaram maior polimorfismo e consistência. Os dados moleculares foram transformados em matriz binária e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Compatibilidade vegetativa; Marcadores moleculares; Variabilidade; AFLP.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000500002

Polimorfismo do fungo simbionte de formigas cortadeiras submetido à luz ultravioleta
Provedor de dados: 65
Autores: Borba,Regina da Silva; Loeck,Alci Enimar; Branco,Juliana Severo Castelo; Kopp,Maurício Marini; Oliveira,Antônio Costa de.
O presente trabalho teve por objetivo verificar o efeito de diferentes períodos de luz ultravioleta sobre a similaridade genética do fungo simbionte de formigas cortadeiras pertencentes às espécies Acromyrmex ambiguus, Acromyrmex crassispinus e Acromyrmex lundi, através da técnica AFLP. Os fungos foram inoculados em meio de cultura Pagnocca e mantidos em câmaras climatizadas à temperatura de 25 + 1°C e escotofase de 24h, por um período de 15 dias. Posteriormente, foram expostos à luz ultravioleta por períodos de: 0, 5, 10, 15, 20, 25 e 30 minutos. Aos 45 dias após o tratamento, foi realizada a extração do DNA e padronização, realizando-se as reações de PCR por meio de 24 combinações de primers AFLP, das quais quatro foram selecionadas por apresentarem...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Marcadores moleculares; Simbiose.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000500001

Sexual reproduction in freshwater fairy shrimp, Dendrocephalus brasiliensis (Pesta, 1921) inferred by Amplified Fragment Length Polymorphism markers
Provedor de dados: 65
Autores: Silva,Suzianny Maria Bezerra Cabral da; Santos,Leilane Bruna Gomes dos; Lopes,José Patrocínio; Gálvez,Alfredo Olivera; Coimbra,Maria Raquel Moura.
Fluctuations in world harvests of Artemia sp cysts have increased prices sharply in the past years. Several organisms have been tested as alternative sources for a total or partial replacement of Artemia sp, among which the microcrustacean (Anostraca) Dendrocephalus brasiliensis stands out. The objective of this study was to investigate the reproductive strategy of D. brasiliensis by means of AFLP markers. The distinction between sexual and parthenogenetic reproduction was conducted using 7 EcoRI and MseI primer combinations in a full-sib family composed of 13 progenies. Forty polymorphic markers were obtained, of these, 35% were from paternal origin and 65% were maternally inherited. Results showed mendelian segregation in all markers through the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcrustacea; AFLP; Paternal inheritance; Sexual reproduction.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000600021

Variabilidade morfocultural e genética de fungos associados a podridão peduncular do mamão
Provedor de dados: 64
Autores: Peres,Ângela Pimenta; Silva-Mann,Renata; Vieira,Maria das Graças G. C.; Machado,José da Cruz.
Objetivou-se, com este trabalho, verificar a variabilidade morfocultural e genética de fungos associados à podridão peduncular do mamão (Carica papaya L.). As avaliações morfoculturais foram feitas pelo índice de crescimento micelial e características das colônias, como coloração e tipo de micélio, quando os isolados foram crescidos em diferentes meios de cultura (BDA, MDA e Czapek). A variabilidade genética foi verificada por meio de análises de AFLP. Os isolamentos foram feitos em frutos obtidos de diferentes regiões produtoras do Brasil. Foram estudados oito isolados de cada uma das três espécies mais incidentes (Colletotrichum gloeosporioides, Phoma caricae-papayae e Fusarium solani). Foi verificado que os meios BDA e MDA promoveram um maior e mais...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colletotrichum gloeosporioides; Phoma caricae-papayae; Fusarium solani; AFLP; Carica papaya.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000500012