Consulta: subjectFacets:"Cytokines"
Registros recuperados: 102
Data/hora: 04/06/2024 14:25:06

Neoangiogenesis Induced by Progenitor Endothelial Cells: Effect of Fucoidan from Marine Algae
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boisson-vidal, C; Zemani, F; Calliguiri, G; Galy-fauroux, I; Colliec-jouault, Sylvia; Helley, D; Fischer, A-m.
Fucoidans -- sulphated polysaccharides extracted from brown algae - could be beneficial in patients with ischemic diseases. Their antithrombotic and proangiogenic properties promote in animals, neovascularization and angiogenesis which prevent necrosis of ischemic tissue. In 1997, endothelial progenitor cells were first identified in human peripheral blood. They are recruited from bone marrow and contribute to neovascularization after ischemic injury. Mobilization of these cells in ischemic sites is an important step in new vessel formation. It is thought that the progenitors interact with endothelial cells, then extravasate and reach ischemic sites, where they proliferate and differentiate into new blood vessels. Although chemokines, cytokines and...
Tipo: Text
Palavras-chave: Growth factors; Cytokines; Angiogenesis; Neovascularization; Endothelial progenitor cells; Sulphated polysaccharides; Fucoidan.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2168.pdf

THE SYSTEMIC INFLAMMATORY RESPONSE SYNDROME RELATED TO THE RELEASE OF CYTOKINES FOLLOWING SEVERE ENVENOMATION
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: VORONOV,E.; APTE,R. N.; SOFER,S.
Envenomation by insects, snakes, scorpions, and spiders involves the activation of the inflammatory system with the release and activation of pro-inflammatory cytokines, chemotactic mediators, cellular infiltration, and other vasoactive mediators. Activation of the inflammatory system and its cascade of events play a major role in the pathogenesis of envenomation, its clinical picture, and outcome. Additional clinical and laboratory studies are required to characterize the exact mechanisms by which the inflammatory system affects the pathophysiology, clinical course, and complications following envenomations. A better understanding of the involvement of the inflammatory cascade in different envenoming syndromes may have future therapeutic benefits.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic inflammatory response; Cytokines; Pro-inflammatory cytokines; Chemotactic mediators; Vasoactive mediators.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301999000100002

ACUTE-PHASE REACTIONS, INCLUDING CYTOKINES, IN PATIENTS BITTEN BY BOTHROPS AND CROTALUS SNAKES IN BRAZIL
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: BARRAVIERA,B.; LOMONTE,B.; TARKOWSKI,A.; HANSON,L. Å.; MEIRA,D.A.
Thirty-one patients bitten by venomous snakes in Botucatu area (State of São Paulo - Brazil), sixteen by Bothrops spp. and fifteen by Crotalus durissus terrificus, were studied. The group comprised twenty-nine males and two females, ranging from fourteen to sixty-three years of age (mean 33 ± 15). Leukocytosis with neutrophilia and lymphopenia, increase of mucoproteins and C-reactive protein, decrease of total serum protein and albumin, were observed on the first day after the accident. In addition, increased serum levels of the cytokines IL-6 and IL-8, but not of IL-1&lt;FONT FACE="Symbol"&gt;b&lt;/FONT&gt; and TNF-&lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;, were observed. The alterations were generally more intense in patients bitten by Crotalus durissus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Acute-phase reactions; Cytokines; C-reactive protein; Inflammation.
Ano: 1995
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301995000100003

Intestinal parasitic infections in HIV/AIDS patients: epidemiological, nutritional and immunological aspects
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Amâncio,FAM; Pascotto,VM; Souza,LR; Calvi,SA; Pereira,PCM.
This study applied a socioeconomic questionnaire designed to evaluate the frequency of intestinal parasites and characterize epidemiological, nutritional, and immunological variables in 105 HIV/AIDS patients - with and without parasitic infections, attending the Day Hospital in Botucatu, UNESP, from 2007 to 2008. Body mass index was calculated and the following tests performed: parasitological stool examinations; eosinophil, IgE, CD4+ T and CD8+ T lymphocyte cell counts; albumin test; viral load measure; and TNF-α, IFN-γ, IL-2, IL-5 and IL-10 cytokine levels. Results were positive for parasitic intestinal infections in 12.4% of individuals. Most patients had good socioeconomic conditions with basic sanitation, urban dwellings, treated water supply and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV/AIDS; Enteroparasites; Nutrition; Immunology; Cytokines.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200013

Antibody and cytokine serum levels in patients subjected to anti-rabies prophylaxis with serum-vaccination
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Ayres,J. A.; Barraviera,B.; Calvi,S. A.; Carvalho,N. R.; Peraçoli,M. T. S.
Rabies is considered a fatal disease once clinical symptoms have developed. The aim of this study was to evaluate epidemiological aspects and immune response in patients attacked by domestic and wild animals and subjected to post-exposure rabies treatment with equine serum and associated vaccine. Thirty-three patients were evaluated; they were between 13 and 65 years old, 75.8% were male and 24.2% female, and from the Botucatu neighborhood. Twenty healthy control individuals with the same age range were also studied. Specific antibodies to equine immunoglobulins and IFN-gamma, IL-2, IL-4, and IL-10 production were evaluated by ELISA. IgM, IgE, IgG and subclasses, and rabies virus antibodies serum levels were determined by nephelometry and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Anti-rabies prophylaxis; Immunoglobulins; Cytokines.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300008

Cytokine profile and clinical metabolic alterations in HIV-1 infected individuals with and without lipodistrophy
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Pontes-Cardoso,L. C. R.; Souza,L. R.; Peraçoli,M. T. S.; Pereira,P. C. M.
The extensive use of Highly Active Antiretroviral Therapy (HAART) has transformed HIV infection into a chronic condition. Thus, metabolic alterations including lipodystrophy and dyslipidemia have been associated with the use of such medications. The objective of the present study was to analyze clinical metabolic alterations and the profile of TNF-alpha, IFN-Y, IL-2, IL-10, and TNF-alpha type II soluble receptor in serum of HIV-1 individuals with and without lipodystrophy. Eighty-four adults were evaluated, 42 males and 42 females, mean age 37 years, and HAART time of at least 15 months. Two groups were formed, G1: 42 individuals with lipodystrophy, and G2: 42 without lipodistropy. From the HAART used, stavudine was more associated with the lipodystrophy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipodystrophy; HIV-1; Hypertriglyceridemia; Cytokines.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200009

Cytokine and antibody production during murine leptospirosis
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Marinho,M.; Silva,C.; Lima,V. M. F.; Peiró,J. R.; Perri,S. H. V.
The aim of the present study was to investigate the kinetics of humoral and cellular responses during leptospirosis. We observed that the presence of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) was associated with antibody production and bacterial recovery, and the compromising of both TNF-alpha and IL-6 in the immunopathogenesis of leptospirosis during an experimental infection of BALB/c mice inoculated with Leptospira interrogans serovar Canicola was verified. Results showed higher levels of TNF-alpha and IL-6 in the initial phase of infection, in which the greatest bacterial clearance was observed. However, when the bacterial recovery was compared with the kinetics of the production of antibodies, the results revealed a kinetics...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Cellular immune response; BALB/c mice; Leptospirosis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400006

Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Gatto,Mariana; Oliveira,Larissa Ragozo Cardoso; Dias,Fernanda De Nuzzi; Araújo Júnior,João Pessoa; Lima,Carlos Roberto Gonçalves; Lordelo,Eliana Peresi; Santos,Rodrigo Mattos dos; Kurokawa,Cilmery Suemi.
Abstract  Background The present study evaluated the effect of treatment with benznidazole on mRNA expression of IFN-γ, IL-17, IL-10, TGF-β and FoxP3 in spleen and heart tissue of BALB/c mice in the acute phase of an experimental infection with Trypanosoma cruzi, strains JLP or Y.  Methods The mRNA expression of cytokines and parasite load were assessed by q-PCR. Dependent groups were compared using Student's paired t-test and independent groups were compared using Student's unpaired t-test.  Results Infection with the JLP or Y strains increased expression of IFN-γ in the heart and of IL-10 and IL-17 in the spleen and heart compared to uninfected animals. Treatment increased the expression of IFN-γ and decreased the expression of IL-17, IL-10, TGF- β and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chagas disease; Trypanosoma cruzi; Immune response; Cytokines; Q-PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100320

Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Marinho,M.; Langoni,H.; Oliveira,S. L.; Lima,V. M. F.; Peiró,J. R.; Perri,S. H. V.; Carreira,R.
Immune response to leptospirosis is mainly humorally mediated, and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. However, some aspects are still unknown. For a more detailed analysis of the cellular immune response to leptospirosis infection, trials were carried out in order to determine the hydrogen peroxide and nitric oxide (H2O2 and NO) production stimulated or not by Interferon-gamma. The participation of some specific cytokines, such as Tumor Necrosis Factor-alpha (TNF-alpha); Interferon-gamma (IFN-gamma); Interleukin-6 (IL-6); and Interleukin-4 (IL-4), in the immunopathology of this infection was also investigated. For this purpose, we analyzed the supernatant from peritoneal macrophage cell culture and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Cytokines; Cellular immunity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200009

Immune reconstitution in HIV-1 infected patients treated for two years with highly active antiretroviral therapy
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Almeida,R. A. M. B.; Souza,L. R.; Calvi,S. A.; Ikoma,M. R. V.; Silva,V. A.; Curi,P. R.; Meira,D. A.
The aim of this paper was to evaluate the immune reconstitution of HIV-1 patients subjected to highly active antiretroviral therapy (HAART) for two years or more according to CD45RA and CD45RO cell count; determination of IL-2, IFN-gamma, IL-4, IL-10 and TNF-alpha serum levels; CD4+ T and CD8+ T lymphocyte count; and plasma viral load (VL) determination. For this purpose, a cross sectional study was carried out in the Tropical Diseases Area, Botucatu School of Medicine, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil. Between June 2001 and April 2002, 37 HIV-1 infected patients were evaluated, 13 with treatment indication but untreated (G1), 9 subjected to HAART for 5-7 months (G2), and 15 treated for two years or more (G3); both treated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Immune reconstitution; Antiretroviral; HAART; Cytokines; CD45RA; CD45RO.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100008

Effects of atropine and propranolol on lung inflammation in experimental envenomation: comparison of two buthidae venoms
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Saidi,Hadjer; Adi-Bessalem,Sonia; Hammoudi-Triki,Djelila; Laraba-Djebari,Fatima.
Background Previous works had shown that scorpion venom induced neurotransmitter elevation and an inflammatory response associated with various anatomo-pathological modifications. The most dangerous scorpions species in Algeria responsible for these effects are Androctonus australis hector (Aah) and Androctonus amoreuxi (Aam). Results Comparison of the physiopathological effects induced by the two venoms showed differences in the kinetic of cytokine release and in lung injury. The lung edema was only observed in response to Aah venom and it was correlated with cell infiltration. In order to better understand the involved mechanism in inflammatory response, we used two antagonists, atropine (non-selective muscarinic antagonist) and propranolol (β adrenergic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venoms; Cytokines; Lung inflammation; Acetylcholine; Atropine; Propranolol.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100305

Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Meira,DA; Almeida,RAMB; Barbosa,AN; de Souza,LR; Olivo,TET; Henriques,RMS; Golim,MA; Araújo Jr,JP; Nagoshi,LR; Orikaza,CM; Calvi,SA.
A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G1 = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G2 = 27); and patients on HAART with undetectable VL for at least the past six months (G3 = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G4 = 20), which was the control group. Serum cytokine levels (values in pg/mL) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RT-PCR; ELISA; Cytokines; HIV; AIDS; HAART; Apoptosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400011

Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Pereira,Andreia Vieira; Barros,Gustavo de; Pinto,Erika Gracielle; Tempone,Andre Gustavo; Orsi,Ricardo de Oliveira; Santos,Lucilene Delazari dos; Calvi,Sueli; Ferreira Jr,Rui Seabra; Pimenta,Daniel Carvalho; Barraviera,Benedito.
Abstract  Background Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases.  Methods  Africanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages was evaluated through mitochondrial oxidative activity. The production of anti- and pro-inflammatory cytokines, NO and H2O2 by macrophages was determined.  Results  Promastigotes and intracellular amastigotes were susceptible to melittin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melittin; Apis mellifera; Leishmania; Leishmaniasis; Peptides; Toxins; Antiparasitic; Cytokines.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100301

Avian cytokines in health and disease
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Wigley,P; Kaiser,P.
Cytokines are proteins secreted by cells that play an important role in the activation and regulation of other cells and tissues during inflammation and immune responses. Although well described in several mammalian species, the role of cytokines and other related proteins is poorly understood in avian species. Recent advances in avian genetics and immunology have begun to allow the exploration of cytokines in health and disease. Cytokines may be classified in a number of ways, but may be conveniently arranged into four broad groups on the basis of their function. Proinflammatory cytokines such as interleukin-6 and interleukin-1beta play a role in mediating inflammation during disease or injury. Th1 cytokines, including interleukin-12 and interferon-gamma,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Immunity; Infectious disease; Inflammation; Vaccination.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000100001

Effects of Gaba on the Thymus Cytokines of Wenchang Chickens Submitted to Heat Stress
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Xie,XZ; Liang,C; Li,MH; Chen,Z.
ABSTRACT Under routine feeding conditions, 1-day-old male chicks were randomly divided into control group (CK), heat-stress group (HS), and GABA + heat stress group (GABA+HS). The thymus contents of IL-1, IL-2, TGF-β1, IFN-γ, GH and HSP70 were determined by enzyme-linked immunosorbent assays. The results showed that: (1) IL-1 and TGF-β1 contents of HS group were significantly lower than those of the CK group (p&lt;0.05), and those of the GABA+HS group were significantly higher relative to the HS group (p&lt;0.05); (2) IL-2 and IFN-γ contents of the HS group were significantly higher than those of the CK group (p&lt;0.05), and those of the GABA+HS group were significantly lower relative to the HS group (p&lt;0.05);(3) the thymus GH content of all three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heat stress; GABA; Cytokines; Thymus; Chicks.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100143

Influence of Covering Reused Broiler Litter with Plastic Canvas on Litter Characteristics and Bacteriology and the Subsequent Immunity and Microbiology of Broilers
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Mesa,D; Lourenço,M; Souza,A; Bueno,A; Pereira,A; Sfeir,M; Santin,E.
ABSTRACT In broiler production, the litter is reused for consecutives flocks, and it is treated during down time between flocks to reduce its microbial load. Although covering the litter with a plastic canvas is a common litter treatment in the field, there is little scientific information available on its efficacy. The aim of this study was to evaluate the effects of covering broiler litter with a plastic canvas for eight days on litter microbiological, physical, and chemical parameters, and on the intestinal microbiota and immunity of broilers. In the first trial, reused litter from a previous flock was distributed into three treatments, with six replicates each: L1 (negative control, litter free from Salmonella Enteritidis (SE) and Eimeria maxima (EM)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ammonia; Cytokines; Lactobacillus; Macrophages; Salmonella.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400563

Soybean β-conglycinin Induces Intestinal Immune Responses in Chicks
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Kang,DR; Belal,SA; Song,KD; Yoon,C; Park,BY; Shim,KS.
ABSTRACT β-conglycinin from soybean has been recognized as one of the major feed allergens. This study investigated the effects of β-conglycinin-induced allergic sensitization on chicks’ small intestines. A total of 40 7-day-old (100 g) chicks were divided into four groups as control, β-conglycinin 1 h, β-conglycinin 6 h, and β-conglycinin 12 h. All treatment groups were administered 60 mg of β-conglycinin/chick and small intestine samples were collected. β-conglycinin-induced allergic sensitization marginally damages the epithelium lining of the duodenum villi and, in addition, significantly increases the accumulation of mast cells in the lamina propria and crypt of the duodenum. Moreover, the TNF-α level significantly increased in all β-conglycinin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-conglycinin; Mucosal immune response; Cytokines; Chick.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000200313

Neurotrophins, cytokines, oxidative parameters and funcionality in Progressive Muscular Dystrophies
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: COMIM,CLARISSA M.; MATHIA,GISIANE B.; HOEPERS,ANDREZA; TUON,LISIANE; KAPCZINSKI,FLÁVIO; DAL-PIZZOL,FELIPE; QUEVEDO,JOÃO; ROSA,MARIA I.
We investigated the levels of brain derived-neurotrophic factor (BDNF), cytokines and oxidative parameters in serum and tried to correlate them with the age and functionality of patients with Progressive Muscle Dystrophies (PMD). The patients were separated into six groups (case and controls pared by age and gender), as follows: Duchenne Muscular Dystrophy (DMD); Steinert Myotonic Dystrophy (SMD); and Limb-girdle Muscular Dystrophy type-2A (LGMD2A). DMD patients (±17.9 years old) had a decrease of functionality, an increase in the IL-1β and TNF-α levels and a decrease of IL-10 levels and superoxide dismutase activity in serum. SMD patients (±25.8 years old) had a decrease of BDNF and IL-10 levels and superoxide dismutase activity and an increase of IL-1β...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Progressive Muscular Dystrophies; BNDF; Cytokines; Oxidative stress.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401809

Balanced cytokine-producing pattern in mice immunized with an avirulent Trypanosoma cruzi
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Soares,Milena B. P.; Gonçalves,Renata; Pyrrho,Alexandre S.; Costa,Deise A.; Paiva,Claudia N.; Gattass,Cerli R.
We have previously demonstrated that inoculation of BALB/c mice with trypomastigotes of CL-14, an avirulent Trypanosoma cruzi clone, prevents the development of parasitemia and mortality after challenge with virulent CL strain. In this report, we investigated the cytokine and antibody profiles induced by inoculation with CL-14 clone. Groups of mice were inoculated with trypomastigotes of CL-14 clone and challenged with infective CL strain. Challenged CL-14-inoculated mice had lower levels of IFN-&lt;FONT FACE=Symbol&gt;g&lt;/font&gt; and higher production of IgG1 antibodies as compared to CL strain-infected mice. Previous inoculation with CL-14 clone partially prevented the suppression of IL-2 production caused by CL strain infection. No significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Protection; Cytokines; Humoral response.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000200005

Understanding the mechanisms of glutamine action in critically ill patients
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Oliveira,Gisele P.; Dias,Cristina M.; Pelosi,Paolo; Rocco,Patricia R.M.
Glutamine (Gln) is an important energy source and has been used as a supplementary energy substrate. Furthermore, Gln is an essential component for numerous metabolic functions, including acid-base homeostasis, gluconeogenesis, nitrogen transport and synthesis of proteins and nucleic acids. Therefore, glutamine plays a significant role in cell homeostasis and organ metabolism. This article aims to review the mechanisms of glutamine action during severe illnesses. In critically ill patients, the increase in mortality was associated with a decreased plasma Gln concentration. During catabolic stress, Gln consumption rate exceeds the supply, and both plasma and skeletal muscle pools of free Gln are severely reduced. The dose and route of Gln administration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heat shock protein; Apoptosis; Cytokines; Glutamine.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000200018

Effects of Aliskiren, an RAAS inhibitor, on a carrageenan-induced pleurisy model of rats
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: BAYIR,YASIN; UN,HARUN; CADIRCI,ELIF; AKPINAR,EROL; DIYARBAKIR,BUSRA; CALIK,ILKNUR; HALICI,ZEKAI.
Abstract Our aim is to investigate the potentially preventive effects of Aliskiren in a carrageenan-induced lung pleurisy model and to compare the standard anti-inflammatory agents, indomethacin and dexamethasone. The pleurisy model was induced through the injection of carrageenan (0.2 ml-%2) into the pleural cavity. After the experiment, serum and lung tissues were collected and biochemical, molecular and pathological examinations were performed. In our study, pleural inflammation decreased superoxide dismutase activity and the glutathione level and increased the malondialdehyde level in the lung of rats, while Aliskiren increased the superoxide dismutase activity and glutathione level and decreased the malondialdehyde level. In addition,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aliskiren; Cytokines; Lung injury; Oxidative stress; Pleurisy; Rat.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100801

In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive review
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: CECHIM,GIOVANA; CHIES,JOSÉ A.B.
Abstract: Dendritic cells (DCs) initiate and shape both innate and adaptive immune responses. They are specialized in antigen presentation to naive T cells, thereby orchestrating the T cell immune responses. Human peripheral blood and tissues contain several subsets of phenotypically and functionally distinct DCs, which promote interactions between the external environment and lymphoid organs. Because of the difficulty in purifying these cells, in vitro studies only became more frequent when Frederica Sallusto and Antonio Lanzavecchia developed a method to generate DCs from blood monocytes in vitro. Nowadays a wide range of biotechnological innovations has allowed the study of DCs and their precursors in the most diverse situations faced by the immune...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Dendritic cell; Differentiation; Monocytes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700614

Early fragmentation of polyester urethane sheet neither causes persistent oxidative stress nor alters the outcome of normal tissue healing in rat skin
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: SGROTT,SANDRO M.; NEVES,RODRIGO D.; D’ACAMPORA,ARMANDO J.; BERNARDES,GERALDO J.S.; BELMONTE,LUIZ; MARTINS,THIAGO C.; BOBINSKI,FRANCIANE; CARGNIN-FERREIRA,EDUARDO; HOEPERS,ANDREZA; COMIM,CLARISSA M.; MARTINS,DANIEL F.; PIOVEZAN,ANNA P.
Abstract Silicone breast implant is associated with complications inherent to the surgical procedure. Prosthesis coating with polyurethane, however, commonly reduces the incidence of such complications. In this paper, the authors evaluated the inflammatory histomorphometric profile and oxidative damage associated to the implant of polyester urethane sheets. Forty-eight Wistar rats were divided into Sham or polyester urethane groups (n = 8/group) and underwent a polyester urethane implant in the dorsal skinfold. Tissue samples were collected on days seven, 30, and 90 after surgery and subjected to histomorphometric analysis and biochemical tests. Results were analyzed by one-way ANOVA (p ≤ 0.05). Peri-implant tissue samples exhibited characteristic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyurethane; Inflammation; Cytokines; Oxidative stress; Wistar rats.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502211

Clinical and laboratory aspects of common variable immunodeficiency
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Kokron,Cristina M.; Errante,Paolo R.; Barros,Myrthes T.; Baracho,Gisele V.; Camargo,Maristela M.; Kalil,Jorge; Rizzo,Luiz V.
Common variable immunodeficiency (CVID) is an immunological disorder characterized by defective antibody production, recurrent infections, most notably of the respiratory tract, autoimmune phenomena and cancer. Some CVID patients may also present disturbances of the cellular immune response such as a decrease in the number and proportion of different lymphocyte populations, diminished lymphoproliferative response to mitogens and antigens, altered production of cytokines, and deficient expression of cell-surface molecules. Most Brazilian CVID patients included in this study show a decrease in T and B lymphocyte counts in the peripheral blood. Furthermore, their lymphocytes are more susceptible to apoptosis following activation than normal individuals, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunodeficiency; Humans; Antibody; Cytokines; Clinical features.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000400007

Saccharomyces boulardii modulates and improves the immune response to Bovine Herpesvirus type 5 Vaccine
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Roos,T.B.; Avila,L.F.C.; Sturbelle,R.T.; Leite,F.L.L.; Fischer,G.; Leite,F.P.L.
ABSTRACT There have been significant efforts towards the development of more efficient vaccines for animal health. A strategy that may be used to improve vaccine efficacy is the use of probiotics to enhance the immune response of the host, leading to increased immunogenicity of antigen preparations. Bovine herpesvirus 5 (BoHV-5) is an example of an important animal pathogen for which vaccines have provided only limited protection. In this study, we examined the use of the probiotic Saccharomyces boulardii (Sb) as a potential adjuvant to improve vaccine efficiency. We found that the supplemented animals exhibited an enhanced systemic IgG antibody response toward a Th1 response in favor of IgG2a and increased mRNA expression levels of the cytokines IFN-y,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immune modulation; Probiotic; Cytokines; Vaccine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000200375

Interleukin-1&amp;#946; regulates metalloproteinase activity and leptin secretion in a cytotrophoblast model
Provedor de dados: Biocell
Autores: Fontana,Vanina Andrea; Sanchez,Melisa; Cebral,Elisa; Calvo,Juan Carlos.
Implantation is one of the most regulated processes in human reproduction, by endocrine and immunological systems. Cytokines are involved in embryo-maternal communication and an impaired balance could result in pregnancy loss. Here we investigated the effect of interleukin 1-&amp;#946; on the activity of two important metalloproteinases (MMP-2 and MMP-9) that are involved in extracellular matrix remodeling as well as the secretion of leptin, one of the reproductive hormones actively regulating their activity and secretion. We found that IL-1&amp;#946; activates matrix metalloproteinase activity as well as increases leptin secretion. We propose that this interleukin, through the regulation of leptin, in turn activates matrix metalloproteinases which results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Embryo implantation; Placenta; Extracellular matrix.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100005

Toll-like Receptors are Key Participants in Innate Immune Responses
Provedor de dados: Biological Research
Autores: ARANCIBIA,SERGIO A; BELTRÁN,CAROLL J; AGUIRRE,ISABEL M; SILVA,PAULINA; PERALTA,ALEXIS L; MALINARICH,FRANO; HERMOSO,MARCELA A.
During an infection, one of the principal challenges for the host is to detect the pathogen and activate a rapid defensive response. The Toll-like family of receptors (TLRs), among other pattern recognition receptors (PRR), performs this detection process in vertebrate and invertebrate organisms. These type I transmembrane receptors identify microbial conserved structures or pathogen-associated molecular patterns (PAMPs). Recognition of microbial components by TLRs initiates signaling transduction pathways that induce gene expression. These gene products regulate innate immune responses and further develop an antigen-specific acquired immunity. TLR signaling pathways are regulated by intracellular adaptor molecules, such as MyD88, TIRAP/Mal, between others...
Tipo: Journal article
Palavras-chave: Toll-like receptors; Innate Immunity; Cytokines; MyD88; NFkB.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200001

Caveolin-1-mediated post-transcriptional regulation of inducible nitric oxide synthase in human colon carcinoma cells
Provedor de dados: Biological Research
Autores: FELLEY-BOSCO,EMANUELA; BENDER,FLORENT; QUEST,ANDREW F.G.
Reactive oxygen species are now widely recognized as important players contributing both to cell homeostasis and the development of disease. In this respect nitric oxide (NO) is no exception. The discussion here will center on regulation of the inducible form of nitric oxide synthase (iNOS) for two reasons. First, only iNOS produces micromolar NO concentrations, amounts that are high by comparison with the picomolar to nanomolar concentrations resulting from Ca2+-controlled NO production by endothelial eNOS or neuronal nNOS. Second, iNOS is not constitutively expressed in cells and regulation of this isoenzyme, in contrast to endothelial eNOS or neuronal nNOS, is widely considered to occur at the transcriptional level only. In particular, we were...
Tipo: Journal article
Palavras-chave: Inducible nitric oxide synthase; Proteosome_mediated proteolysis; Nitroxysomes; Cytokines; Caveolae; Caveolin-1.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200007

Higher than normal plasma Iinterleukin-6 concentrations in brazilian patients with mood disorders
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Miranda,Helen Cristina; Nunes,Sandra Odebrecht Vargas; Reiche,Edna Maria Vissoci; Oda,Julie Massayo Maeda; Watanabe,Maria Angelica Ehara.
The aim of this work was to study the plasma concentration of IL-6 by ELISA in the patients with mood disorders and normal healthy donors. The plasma concentration of IL-6 was higher in the patients than in the control healthy group. Results suggested that IL-6 could serve as an immunological marker in mood disorder pathogenesis as well.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IL-6; Cytokines; Mood disorder.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400010

Propionibacterium acnes-killed attenuates the inflammatory response and protects mice from sepsis by modulating inflammatory factors
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Silva,José Bruno Nunes Ferreira da; Oliveira,Samara Kelly Mendonça de; Campos,Ingrid Araújo; Carvalho-Júnior,Carlson Helder Reis de; Coutinho,Thiago da Cunha; Silva,Teresinha Gonçalves.
BACKGROUND: Sepsis is a systemic inflammation associated with infection caused by pathogenic micro-organisms with high mortality rates. OBJECTIVE: In this study, we investigated the protective effect of Propionibacterium acnes-killed against polymicrobial sepsis induced by cecal ligation and puncture. METHODS: The mice were treated by intramuscular route in 1, 3, 5, and 7 days before the cecal ligation and puncture induction. The control group animals received vehicle (saline solution 0.9%) and the animals of the treated group received the P. acnes-killed (0.4 mg/animal). After anesthesia, midline laparotomy was performed with exposure of cecum followed by ligature and one transverse perforation of the same, with a 18 G needle, for induction of lethal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propionibacterium acnes; Immunomodulation; Sepsis; Cytokines.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000100004

Correlation between serum tumor necrosis factor alpha levels and clinical severity of tuberculosis
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Andrade Júnior,Dahir Ramos de; Santos,Sânia Alves dos; Castro,Isac de; Andrade,Dahir Ramos de.
This study verified the correlation between the serum levels of TNF alpha and different clinical forms of tuberculosis. We described a group of 24 patients presenting several clinical forms of tuberculosis and a control group of 13 healthy individuals. The levels of TNF alpha were measured by bioassay method. The levels of TNF-alpha had significant differences between the tuberculosis and control groups. The patients with abnormal chest X-Ray findings had higher TNF alpha levels (15328.48 ± 4602.19 pg/mL) when compared to patients with normal X-Rays (3353.18 ± 1495.29 pg/mL) (p&lt;0.05). Patients that lost weight had higher TNF alpha levels (15468.54 ± 4580.54 pg/mL) than those that didn't loose weight (2904.98 ± 1367.89) (p&lt;0.05). The levels of TNF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TNF alpha; Tuberculosis; Cytokines; Clinical severity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300013

Propolis derivatives inhibit the systemic inflammatory response and protect hepatic and neuronal cells in acute septic shock
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Korish,Aida Abdelhamid; Arafa,Maha Mohamed.
BACKGROUND: Severe pathogenic infection triggers excessive release of cytokines as part of the massive inflammatory response associated with septic shock. OBJECTIVES: To investigate the protective effect of caffeic acid phenethye ester (CAPE) against lipopolysaccharide (LPS) induced endotoxemia, hepatic and neuronal damage and the associated systemic inflammatory response (SIR). METHODS: Fifty male Wister rats were divided into: control, LPS, and CAPE+LPS groups. Plasma concentrations of various cytokines, including TNF-α, IL-1α, IL-1β, IL-6, IL-4, IL-10, and sICAM-1 were evaluated. In addition, the histopathological changes in the hepatic and neural cells were assessed. RESULTS: The LPS group showed high inflammatory cytokines and sICAM-1 levels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipopolysaccharides; Septic shock; Systemic inflammatory response syndrome; Cytokines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400006

Immune cellular response to HPV: current concepts
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Gonçalves,Maria Alice Guimarães; Donadi,Eduardo Antonio.
Although cellular immunity is essential for the elimination of human papillomavirus (HPV), the mechanisms involved are still poorly understood. We summarize the main mechanisms involved in cellular immune response to infections caused by HPV. Immunotherapies for HPV-related cancers require the disruption of T-cell response control mechanisms, associated with the stimulation of the Th1 cytokine response.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus (HPV); Cervical intraepithelial neoplasia (CIN); Uterine cervical cancer; Cellular immune response; Cytokines.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100001

T helper type cytokines in sepsis: time-shared variance and correlation with organ dysfunction and hospital mortality
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Costa,Ramon Teixeira; Araújo,Orlei Ribeiro de; Brunialti,Milena Karina Coló; Assunção,Murilo Santucci Cesar; Azevedo,Luciano César Pontes; Freitas,Flávio; Salomão,Reinaldo.
ABSTRACT  Objective: We evaluated the kinetics of cytokines belonging to the T helper1 (Th1), Th2, and Th17 profiles in septic patients, and their correlations with organ dysfunction and hospital mortality.  Methods: This was a prospective observational study in a cohort of septic patients admitted to the intensive care units (ICU) of three Brazilian general hospitals. A total of 104 septic patients and 53 health volunteers (controls) were included. Plasma samples were collected within the first 48 h of organ dysfunction or septic shock (0D), after seven (D7) and 14 days (D14) of follow-up. The following cytokines were measured by flow cytometry: Interleukin-1β (IL-1β), IL-2, IL-6, IL-8, IL-10, IL-12/23p40, IL-17, IL-21, tumor necrosis factor-α (TNF-α),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sepsis; Cytokines; Th response; Organ dysfunction; SOFA.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000200079

Dexmedetomidine decreases the inflammatory response to myocardial surgery under mini-cardiopulmonary bypass
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bulow,N.M.H.; Colpo,E.; Pereira,R.P.; Correa,E.F.M.; Waczuk,E.P.; Duarte,M.F.; Rocha,J.B.T.
Cardiopulmonary bypass (CPB) with extracorporeal circulation produces changes in the immune system accompanied by an increase in proinflammatory cytokines and a decrease in anti-inflammatory cytokines. We hypothesize that dexmedetomidine (DEX) as an anesthetic adjuvant modulates the inflammatory response after coronary artery bypass graft surgery with mini-CPB. In a prospective, randomized, blind study, 12 patients (4 females and 8 males, age range 42-72) were assigned to DEX group and compared with a conventional total intravenous anesthesia (TIVA) group of 11 patients (4 females and 7 males). The endpoints used to assess inflammatory and biochemical responses to mini-CPB were plasma interleukin (IL)-1, IL-6, IL-10, interferon (INF)-γ, tumor necrosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Systemic inflammatory response syndrome; Total intravenous anesthesia; Dexmedetomidine; Coronary artery bypass grafting surgery; Mini-cardiopulmonary bypass.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400702

Serum cytokine levels in autoimmune and non-autoimmune hyperthyroid states
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ward,L.S.; Fernandes,G.A.
Although the role of interleukin-2 (IL-2) and interferon gamma (&lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt;IFN) is still poorly understood in hyperthyroid diseases, it is reasonable to assume that these cytokines may be present at higher levels in Graves' disease (GD) than in other primarily non-autoimmune thyroid diseases. In order to look for an easy method to distinguish GD from primarily non-autoimmune causes of hyperthyroidism, we compared 13 healthy individuals with 21 treated and untreated hyperthyroid GD patients and with 19 patients with hyperthyroidism due to other etiologies: 7 cases of multinodular goiter, 5 cases of excessive hormone replacement and 7 cases of amiodarone-associated hyperthyroidism. All patients presented low TSH levels and a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytokines; IL-2; Hyperthyroidism; Graves' disease; Diagnosis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100009

Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) R.M. King &amp;amp; H. Rob. aqueous extract on human lymphocyte proliferation and IFN-γ and TNF-α production in vitro
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Almeida,V.G.; Avelar-Freitas,B.A.; Santos,M.G.; Costa,L.A.; Silva,T.J.; Pereira,W.F.; Amorim,M.L.L.; Grael,C.F.F.; Gregório,L.E.; Rocha-Vieira,E.; Brito-Melo,G.E.A.
Pseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. brasiliensis aqueous extract and its effects on pro-inflammatory cytokine production and lymphocyte proliferation. The extracts were prepared by sequential maceration of P. brasiliensis leaves in ethanol, ethyl acetate, and water. Extract cytotoxicity was evaluated by trypan blue exclusion assay, and apoptosis and necrosis were measured by staining with annexin V-FITC and propidium iodide. The ethanolic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Lymphocyte proliferation; Luteolin; Medicinal plant; Pseudobrickellia brasiliensis; Quinic acid.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800603

Cytokine profile and nitric oxide levels in sera from patients with brucellosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Refik,M.; Mehmet,N.; Durmaz,R.; Ersoy,Y.
The aims of this study were to investigate the serum levels of some cytokines [tumor necrosis factor-alpha (TNF-alpha), interleukin 1ß (IL-1ß), IL-2R, IL-6, and IL-8] and nitric oxide (NO) levels in patients with untreated brucellosis and to test the correlation of these parameters with each other. The study was conducted on 67 subjects, 37 patients with brucellosis and 30 healthy individuals with no history of Brucella infection. Brucellosis was identified by a positive blood culture and/or increased Brucella antibodies in serological tests in addition to compatible clinical symptoms. Cytokine profile analysis was performed by the immulite chemiluminescent enzyme immunometric assay whose inter- and intra-assay coefficients of variance were 2.6-3.6 and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Human brucellosis; Cytokines; Nitric oxide; Interleukin-2; Interleukin-6; Interleukin-8.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100010

Immune response to respiratory syncytial virus in young Brazilian children
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Queiróz,D.A.O.; Durigon,E.L.; Botosso,V.F.; Ejzemberg,B.; Vieira,S.E.; Mineo,J.R.; Yamashita,C.; Hein,N.; Lopes,C.L.; Cacharo,A.L.; Stewien,K.E.
We have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients &lt;=12 months of age (39 males and 26 females, 28 cases &lt;3 months and 37 cases  &gt; or = 3 months; median 3 ± 3.9 months) were tested for anti-RSV IgG and IgG subclass antibodies by EIA. Flow cytometry was used to characterize cell surface markers expressed on peripheral blood mononuclear cells (PBMC) from 29 RSV-infected children. There was a low rate of seroconversion in children &lt;3 months of age, whose acute-phase PBMC were mostly T lymphocytes (63.0 ± 9.0%). In contrast, a higher rate of seroconversion was observed in children &gt;3 months of age, with predominance of B lymphocytes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Respiratory syncytial virus; Peripheral blood mononuclear cells; Young children; Surface antigens; Cytokines.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000011

Effect of chronic ethanol consumption in female rats subjected to experimental sepsis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Castro,C.L.; Aguiar-Nemer,A.S.; Castro-Faria-Neto,H.C.; Barros,F.R.; Rocha,E.M.S.; Silva-Fonseca,V.A.
The objective of this research was to evaluate the interference of ethanol consumption by female rats with cytokines involved in the sepsis process and its correlation with mortality, the main outcome of sepsis. Female Wistar rats in estrus phase were evaluated in three experiments. Experiment 1 (n=40) was performed to determine survival rates. Experiment 2 (n=69) was designed for biochemical analysis, measurement of cytokine and estrogen levels before and after sepsis, and experiment 3 (n=10) was performed to evaluate bacterial growth by colony counts of peritoneal fluid. In all experiments, treated animals were exposed to a 10% ethanol/water solution (v/v) as the single drinking source, while untreated animals were given tap water. After 4 weeks, sepsis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcohol; Sepsis; Cytokines; Estrogens.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201033

Immune and hormonal activity in adults suffering from depression
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Nunes,S.O.V.; Reiche,E.M.V.; Morimoto,H.K.; Matsuo,T.; Itano,E.N.; Xavier,E.C.D.; Yamashita,C.M.; Vieira,V.R.; Menoli,A.V.; Silva,S.S.; Costa,F.B.; Reiche,F.V.; Silva,F.L.V.; Kaminami,M.S.
An association between depression and altered immune and hormonal systems has been suggested by the results of many studies. In the present study we carried out immune and hormonal measurements in 40 non-medicated, ambulatory adult patients with depression determined by CID-10 criteria and compared with 34 healthy nondepressed subjects. The severity of the condition was determined with the Hamilton Depression Rating Scale. Of 40 depressed patients, 31 had very severe and 9 severe or moderate depression, 29 (72.5%) were females and 11 (27.5%) were males (2.6:1 ratio). The results revealed a significant reduction of albumin and elevation of alpha-1, alpha-2 and ß-globulins, and soluble IL-2 receptor in patients with depression compared to the values obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Depression; Immunity; Psychoneuroimmunology; Cytokines; Acute phase proteins.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500011

Cytokine production profile of heart-infiltrating T cells in Chagas' disease cardiomyopathy
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cunha-Neto,E.; Rizzo,L.V.; Albuquerque,F.; Abel,L.; Guilherme,L.; Bocchi,E.; Bacal,F.; Carrara,D.; Ianni,B.; Mady,C.; Kalil,J.
The hallmark of chronic Chagas' disease cardiomyopathy (CCC) is the finding of a T cell-rich inflammatory mononuclear cell infiltrate in the presence of extremely few parasites in the heart lesions. The scarcity of parasites in affected heart tissue casts doubt on the direct participation of Trypanosoma cruzi in CCC heart tissue lesions, and suggests the possible involvement of autoimmunity. The cells in the infiltrate are presumably the ultimate effectors of tissue damage, and there is evidence that such cells recognize cardiac myosin in molecular mimicry with T. cruzi proteins rather than primary reactivity to T. cruzi antigens (Cunha-Neto et al. (1996) Journal of Clinical Investigation, 98: 1709-1712). Recently, we have studied heart-infiltrating T...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Chagas' disease cardiomyopathy; Immunology; Cytokines; Gamma-interferon.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100018

Inflammatory cytokines and plasma redox status responses in hypertensive subjects after heat exposure
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Fonseca,S.F.; Mendonça,V.A.; Teles,M.C.; Ribeiro,V.G.C.; Tossige-Gomes,R.; Neves,C.D.C.; Rocha-Vieira,E.; Leite,L.H.R.; Soares,D.D.; Coimbra,C.C.; Lacerda,A.C.R.
Hypertension is characterized by a pro-inflammatory status, including redox imbalance and increased levels of pro-inflammatory cytokines, which may be exacerbated after heat exposure. However, the effects of heat exposure, specifically in individuals with inflammatory chronic diseases such as hypertension, are complex and not well understood. This study compared the effects of heat exposure on plasma cytokine levels and redox status parameters in 8 hypertensive (H) and 8 normotensive (N) subjects (age: 46.5±1.3 and 45.6±1.4 years old, body mass index: 25.8±0.8 and 25.6±0.6 kg/m2, mean arterial pressure: 98.0±2.8 and 86.0±2.3 mmHg, respectively). They remained at rest in a sitting position for 10 min in a thermoneutral environment (22°C) followed by 30 min...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Heat; Cytokines; Redox status.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300701

Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Lachtermacher,S.; Esporcatte,B.L.B.; Montalvão,F.; Costa,P.C.; Rodrigues,D.C.; Belem,L.; Rabischoffisky,A.; Faria Neto,H.C.C.; Vasconcellos,R.; Iacobas,S.; Iacobas,D.A.; Dohmann,H.F.R.; Spray,D.C.; Goldenberg,R.C.S.; Campos-de-Carvalho,A.C.
After myocardial infarction (MI), activation of the immune system and inflammatory mechanisms, among others, can lead to ventricular remodeling and heart failure (HF). The interaction between these systemic alterations and corresponding changes in the heart has not been extensively examined in the setting of chronic ischemia. The main purpose of this study was to investigate alterations in cardiac gene and systemic cytokine profile in mice with post-ischemic HF. Plasma was tested for IgM and IgG anti-heart reactive repertoire and inflammatory cytokines. Heart samples were assayed for gene expression by analyzing hybridization to AECOM 32k mouse microarrays. Ischemic HF significantly increased the levels of total serum IgM (by 5.2-fold) and total IgG (by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental post-ischemic heart failure; Anti-heart antibodies; Cytokines; Immunoarray; RNAm - Microarray.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400009

Lung hyperinflation stimulates the release of inflammatory mediators in spontaneously breathing subjects
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Malbouisson,L.M.S.; Szeles,T.F.; Barbalho,L.; Massoco,C.O.; Carmona,M.J.C.; Carvalho,C.R.R.; Pelosi,P.; Auler Jr.,J.O.C.
Lung hyperinflation up to vital capacity is used to re-expand collapsed lung areas and to improve gas exchange during general anesthesia. However, it may induce inflammation in normal lungs. The objective of this study was to evaluate the effects of a lung hyperinflation maneuver (LHM) on plasma cytokine release in 10 healthy subjects (age: 26.1 ± 1.2 years, BMI: 23.8 ± 3.6 kg/m²). LHM was performed applying continuous positive airway pressure (CPAP) with a face mask, increased by 3-cmH2O steps up to 20 cmH2O every 5 breaths. At CPAP 20 cmH2O, an inspiratory pressure of 20 cmH2O above CPAP was applied, reaching an airway pressure of 40 cmH2O for 10 breaths. CPAP was then decreased stepwise. Blood samples were collected before and 2 and 12 h after LHM....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung hyperinflation; Ventilator-induced lung injury; Inflammation; Cytokines.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200011

Effects of sciatic-conditioned medium on neonatal rat retinal cells in vitro
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Torres,P.M.M.; Guilarducci,C.V.V.; Araujo,E.G.
Schwann cells produce and release trophic factors that induce the regeneration and survival of neurons following lesions in the peripheral nerves. In the present study we examined the in vitro ability of developing rat retinal cells to respond to factors released from fragments of sciatic nerve. Treatment of neonatal rat retinal cells with sciatic-conditioned medium (SCM) for 48 h induced an increase of 92.5 ± 8.8% (N = 7 for each group) in the amount of total protein. SCM increased cell adhesion, neuronal survival and glial cell proliferation as evaluated by morphological criteria. This effect was completely blocked by 2.5 µM chelerythrine chloride, an inhibitor of protein kinase C (PKC). These data indicate that PKC activation is involved in the effect...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytokines; Neurotrophic factors; Retina; Schwann cells; Sciatic nerve.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100012

Human_Leishmaniasis@cytokines.bahia.br
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Barral-Netto,M.; Brodskyn,C.; Carvalho,E.M.; Barral,A.
The cell-mediated immune response is critical in the resistance to and recovery from leishmaniasis. Cytokines are central elements in mounting an immune response and have received a great deal of attention in both human and experimental leishmaniasis. IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; is responsible for macrophage activation leading to leishmanicidal mechanisms. Understanding the balance of cytokines that lead to enhanced production of or synergize with IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;, and those cytokines that counterbalance its effects is fundamental for developing rational immunotherapeutic or immunoprophylactic approaches to leishmaniasis. Here we focus on the cytokine balance in human leishmaniasis, particularly IL-10 as an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Human leishmaniasis; Immunoregulation; IL-12; IL-10.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100021

Clinical and immunological features of patients with atopy and concomitant HTLV-1 infection
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Gaspar-Sobrinho,F.P.; Souza-Machado,A.; Santos,S.B.; Orge,G.; Lessa,H.A.; Cruz,A.A.; Carvalho,E.M.
Human T-cell lymphotropic virus type 1 (HTLV-1) induces an exacerbated type 1 immune response characterized by high spontaneous IFN-γ and TNF-α production. Allergic rhinitis and asthma are associated with the type 2 immune response, with elevated secretion of IL-4 and IL-5. The aim of this study was to characterize the immune response in atopic HTLV-1 carriers. The cytokine profile of atopic HTLV-1 carriers (N = 10; all females) was compared with that of non-atopic HTLV-1 carriers (N = 14; 9 females and 5 males). Mean patient age of atopic and non-atopic groups was 45 ± 8 and 38 ± 11 years, respectively. All atopic HTLV-1 carriers had rhinitis with or without asthma and a skin prick test positive for Dermatophagoides pteronyssinus antigen 1 (Derp-1). There...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Atopy; Cytokines; HTLV-1; Rhinitis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200005

Ethanol-induced colitis prevents oral tolerance induction in mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Andrade,M.C.; Vaz,N.M.; Faria,A.M.C.
The gut mucosa is a major site of contact with antigens from food and microbiota. Usually, these daily contacts with natural antigens do not result in inflammatory reactions; instead they result in a state of systemic hyporesponsiveness named oral tolerance. Inflammatory bowel diseases (IBD) are associated with the breakdown of the immunoregulatory mechanisms that maintain oral tolerance. Several animal models of IBD/colitis are available. In mice, these include targeted disruptions of the genes encoding cytokines, T cell subsets or signaling proteins. Colitis can also be induced by intrarectal administration of chemical substances such as 2,4,6-trinitrobenzene sulfonic acid in 50% ethanol. We report here a novel model of colitis induced by intrarectal...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ethanol; Colitis; Oral tolerance; Cytokines.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900013

Peritoneal and serum interleukin-18 levels are not increased in women with minimum or mild endometriosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Glitz,C.; Souza,C.A.; Rodini,G.P.; Genro,V.; Bilibio,J.P.; Senger,M.; Cunha-Filho,J.S.
Interleukin-18 (IL-18) is a cytokine that belongs to the IL-1 family. Endometriosis is strongly associated with sub-fertility, and affects about 15% of women of reproductive age. IL-18 may favor the progression of endometriosis. The objective of the present study was to determine the concentration of IL-18 in the serum and peritoneal fluid of infertile women with endometriosis. Forty infertile and 25 fertile women were screened in a teaching hospital. Thirty-four infertile patients with minimal or mild endometriosis and 22 fertile controls were enrolled in the study. The primary outcome was the estimate of IL-18 levels and the secondary outcome was the correlation between serum and peritoneal levels of IL-18. There were no differences between the two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Endometriosis; IL-18; Peritoneal fluid; Infertility.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100007

DNA encoding individual mycobacterial antigens protects mice against tuberculosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Silva,C.L.; Bonato,V.L.D.; Lima,V.M.F.
Over the last few years, some of our experiments in which mycobacterial antigens were presented to the immune system as if they were viral antigens have had a significant impact on our understanding of protective immunity against tuberculosis. They have also markedly enhanced the prospects for new vaccines. We now know that individual mycobacterial protein antigens can confer protection equal to that from live BCG vaccine in mice. A critical determinant of the outcome of immunization appears to be the degree to which antigen-specific cytotoxic T cells are generated by the immune response. Our most recent studies indicate that DNA vaccination is an effective way to establish long-lasting cytotoxic T cell memory and protection against tuberculosis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; DNA vaccination; Cytokines; Th1/Th2.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200012

IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Maciel,M.; Fusaro,A.E.; Oliveira,C.R.; Futata,E.A.; Duarte,A.J.S.; Sato,M.N.
Induced oral tolerance to mucosal-exposed antigens in immunized animals is of particular interest for the development of immunotherapeutic approaches to human allergic diseases. This is a unique feature of mucosal surfaces which represent the main contact interface with the external environment. However, the influence of oral tolerance on specific and natural polyreactive IgA antibodies, the major defense mechanism of the mucosa, is unknown. We have shown that oral administration of an extract of the dust mite Dermatophagoides pteronyssinus (Dp) to primed mice caused down-regulation of IgE responses and an increase in tumor growth factor-ß secretion. In the present study, we observed that primed inbred female A/Sn mice (8 to 10 weeks old) fed by gavage a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucosa; Atural IgA antibodies; Oral tolerance; Cytokines; Allergy; Dermatophagoides pteronyssinus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600006

New strategies in hematopoietic stem cell transplantation: G-CSF-mobilized unprocessed whole blood
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Dräger,A.M.; Ossenkoppele,G.J.; Jonkhoff,A.R.; Schuurhuis,G.J.; Huijgens,P.C.
Transplantation of mobilized peripheral blood stem cells (PBSC) for rescue of bone marrow function after high-dose chemo-/radiotherapy is widely used in hematologic malignancies and solid tumors. Mobilization of stem cells to the peripheral blood can be achieved by cytokine treatment of the patients. The main advantage of autologous PBSC transplantation over bone marrow transplantation is the faster recovery of neutrophil and platelet counts. The threshold number of PBSC required for adequate rescue of bone marrow is thought to be about 2 x 106 CD34+ cells/kg, if the stem cells are collected by leukapheresis and subsequently cryopreserved. We show that this critical number could be further reduced to as few as 0.2 x 106 cells/kg. In 30 patients with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematopoietic stem cells; Transplantation; Cytokines; G-CSF; Lymphoma; Multiple myeloma.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100006

Immunological properties of gene vaccines delivered by different routes
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Oliveira,S.C.; Rosinha,G.M.S.; de-Brito,C.F.A.; Fonseca,C.T.; Afonso,R.R.; Costa,M.C.M.S.; Goes,A.M.; Rech,E.L.; Azevedo,V.
Gene vaccines represent a new and promising approach to control infectious diseases, inducing a protective immune response in the appropriate host. Several routes and methods of genetic immunization have been shown to induce antibody production as well as T helper (Th) cell and cytotoxic T lymphocyte activation. However, few studies have compared the nature of the immune responses generated by different gene vaccination delivery systems. In the present study we reviewed some aspects of immunity induced by gene immunization and compared the immune responses produced by intramuscular (im) DNA injection to gene gun-mediated DNA transfer into the skin of BALB/c mice. Using a reporter gene coding for ß-galactosidase, we have demonstrated that im injection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene vaccination; Naked DNA; Gene gun; Cytokines; Immune response.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200009

Effect of metabolic control on interferon-gamma and interleukin-10 production by peripheral blood mononuclear cells from type 1 and type 2 diabetic patients
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Foss-Freitas,M.C.; Foss,N.T.; Donadi,E.A.; Foss,M.C.
The objective of the present study was to evaluate the production of cytokines, interferon-g (INF-g) and interleukin-10 (IL-10), in cultures of peripheral blood mononuclear cells (PBMC) from type 1 and type 2 diabetic patients and to correlate it with inadequate and adequate metabolic control. We studied 11 type 1 and 13 type 2 diabetic patients and 21 healthy individuals divided into two groups (N = 11 and 10) paired by sex and age with type 1 and type 2 diabetic patients. The PBMC cultures were stimulated with concanavalin-A to measure INF-g and IL-10 supernatant concentration by ELISA. For patients with inadequate metabolic control, the cultures were performed on the first day of hospitalization and again after intensive treatment to achieve adequate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 1 diabetes mellitus; Type 2 diabetes mellitus; Cytokines; Interferon-gamma; Interleukin-10; Metabolic control.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500010

Chemokines, lymphocytes, and HIV
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Farber,J.M.
Chemokines are members of a family of more than 30 human cytokines whose best-described activities are as chemotactic factors for leukocytes and that are presumed to be important in leukocyte recruitment and trafficking. While many chemokines can act on lymphocytes, the roles of chemokines and their receptors in lymphocyte biology are poorly understood. The recent discoveries that chemokines can suppress infection by HIV-1 and that chemokine receptors serve, along with CD4, as obligate co-receptors for HIV-1 entry have lent urgency to studies on the relationships between chemokines and lymphocytes. My laboratory has characterized Mig and Crg-2/IP-10, chemokines that are induced by IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; and that specifically target...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Lymphoid cells; HIV-1; Receptors.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100002

How are antibodies involved in the protective mechanism of susceptible mice infected with T. cruzi?
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Umekita,L.F.; Mota,I.
Host resistance to Trypanosoma cruzi is dependent on both natural and acquired immune responses. During the acute phase of the infection the presence of IFN-&lt;FONT FACE=Symbol&gt;g&lt;/font&gt;, TNF-&lt;FONT FACE=Symbol&gt;a&lt;/font&gt;, IL-12 and GM-CSF has been closely associated with resistance, whereas TGF-ß and IL-10 have been associated with susceptibility. Several investigators have demonstrated that antibodies are responsible for the survival of susceptible animals in the initial phase of infection and for the maintenance of low levels of parasitemia in the chronic phase. However, how this occurs is not yet understood. Our results and other data in the literature support the hypothesis that the protective role of antibodies in the acute phase of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Trypanosoma cruzi; Chronic mouse antibodies; Clearance; Cytokines; Acute phase; Susceptible mice.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300001

Therapeutic effects of Sophora moorcroftiana alkaloids in combination with albendazole in mice experimentally infected with protoscolices of Echinococcus granulosus
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ma,X.M.; Bao,G.SH.; Wan,J.M.; Liao,D.J.; Yin,SH.F.; Meng,X.Q.; Zhou,G.K.; Lu,X.M.; Li,H.Y.
The objective of the present study was to determine if the combination of alkaloids from Sophora moorcroftiana seeds and albendazole might be effective in the treatment of experimental echinococcosisin female NIH mice (6 weeks old and weighing 18-20 g, N = 8 in each group) infected withprotoscolices of Echinococcus granulosus. Viable protoscolices (N = 6 x 103) were cultured in vitro in 1640 medium and mortality was calculated daily. To determine the in vivo efficacy, mice were inoculated intraperitoneally with viable protoscolices and then treated once daily by gavage for three months with the alkaloids (50 mg kg-1 day-1) and albendazole (50 mg kg-1 day-1), separately and in combination (both alkaloids at 25 mg kg-1 day-1 and albendazole at 25 mg kg-1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sophora moorcroftiana; Echinococcus granulosus; Alkaloids; Cytokines; Ethnobotany; Therapeutics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000014

Inflammatory and oxidative stress airway markers in premature newborns of hypertensive mothers
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Madoglio,R.J.; Rugolo,L.M.S.S.; Kurokawa,C.S.; Sá,M.P.A.; Lyra,J.C.; Antunes,L.C.O.
Although oxidative stress and inflammation are important mechanisms in the pathophysiology of preeclampsia and preterm diseases, their contribution to the respiratory prognosis of premature infants of hypertensive mothers is not known. Our objective was to determine the levels of oxidative stress and inflammation markers in the airways of premature infants born to hypertensive and normotensive mothers, in the first 72 h of life, and to investigate whether they are predictors of bronchopulmonary dysplasia (BPD)/death. This was a prospective study with premature infants less than 34 weeks’ gestation on respiratory support who were stratified into 2 groups: 32 premature infants of hypertensive mothers and 41 of normotensive women, with a mean gestational age...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infant; Premature; Pre-eclampsia; Oxidative stress; Cytokines; Bronchopulmonary dysplasia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900703

Extracts of Ascaris suum egg and adult worm share similar immunosuppressive properties
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Souza,V.M.O.; Faquim-Mauro,E.L.; Macedo,M.S.
Adult Ascaris suum body extract (Asc) prepared from male and female worms (with stored eggs) down-regulates the specific immune response of DBA/2 mice to ovalbumin (OA) and preferentially stimulates a Th2 response to its own components, which is responsible for the suppression of the OA-specific Th1 response. Here, we investigated the participation of soluble extracts prepared from male or female worms or from eggs (E-Asc) in these immunological events. Extracts from either sex (1 mg/animal) or E-Asc (0.35 or 1 mg protein/animal) suppressed the delayed-type hypersensitivity (DTH) reaction (60-85%), proliferative response (50-70%), IL-2 and IFN-gamma secretion (below detection threshold) and IgG1 antibody production (70-90%) of DBA/2 mice to OA. A dose of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascaris suum; Immunosuppression; Adult worms; Eggs; Cytokines.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100012

Cytokine profile and lymphocyte subsets in type 2 diabetes
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Francisco,C.O.; Catai,A.M.; Moura-Tonello,S.C.G.; Arruda,L.C.M.; Lopes,S.L.B.; Benze,B.G.; Del Vale,A.M.; Malmegrim,K.C.R.; Leal,A.M.O.
Type 2 diabetes mellitus (T2D) is a metabolic disease with inflammation as an important pathogenic background. However, the pattern of immune cell subsets and the cytokine profile associated with development of T2D are unclear. The objective of this study was to evaluate different components of the immune system in T2D patients' peripheral blood by quantifying the frequency of lymphocyte subsets and intracellular pro- and anti-inflammatory cytokine production by T cells. Clinical data and blood samples were collected from 22 men (51.6±6.3 years old) with T2D and 20 nonsmoking men (49.4±7.6 years old) who were matched for age and sex as control subjects. Glycated hemoglobin, high-sensitivity C-reactive protein concentrations, and the lipid profile were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes mellitus; Cytokines; Lymphocytes; Inflammation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400707

Attenuation of phosphamidon-induced oxidative stress and immune dysfunction in rats treated with N-acetylcysteine
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Suke,S.G.; Ahmed,R.S.; Pathak,R.; Tripathi,A.K.; Banerjee,B.D.
The effect of N-acetylcysteine, a thiolic antioxidant, on attenuation of phosphamidon-induced oxidative stress and immune dysfunction was evaluated in adult male Wistar rats weighing 200-250 g. Rats were divided into four groups, 8 animals/group, and treated with phosphamidon, N-acetylcysteine or the combination of both for 28 days. Oral administration of phosphamidon (1.74 mg/kg), an organophosphate insecticide, increased serum malondialdehyde (3.83 ± 0.18 vs 2.91 ± 0.24 nmol/mL; P &lt; 0.05) and decreased erythrocyte superoxide dismutase (567.8 ± 24.36 vs 749.16 ± 102.61 U/gHb; P &lt; 0.05), catalase activity (1.86 ± 0.18 vs 2.43 ± 0.08 U/gHb; P &lt; 0.05) and whole blood glutathione levels (1.25 ± 0.21 vs 2.28 ± 0.08 mg/gHb; P &lt; 0.05) showing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Antioxidant; Organophosphate; Oxidative stress; Immunotoxicity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900004

Use of inflammatory molecules to predict the occurrence of fever in onco-hematological patients with neutropenia
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ribeiro,A.F. Tibúrcio; Nobre,V.; Neuenschwander,L.C.; Teixeira,A.L.; Xavier,S.G.; Paula,F.D.F.; Teixeira,M.M.; Teixeira,J.C.A.; Bittencourt,H.
Febrile neutropenia remains a frequent complication in onco-hematological patients, and changes in the circulating level of inflammatory molecules (IM) may precede the occurrence of fever. The present observational prospective study was carried out to evaluate the behavior of plasma tumor necrosis factor alpha (TNF-α), soluble TNF-α I and II receptors (sTNFRI and sTNFRII), monocyte chemoattractant protein-1 [MCP-1 or chemokine (c-c motif) ligand 2 (CCL2)], macrophage inflammatory protein-1α (MIP-1α or CCL3), eotaxin (CCL11), interleukin-8 (IL-8 or CXCL8), and interferon-inducible protein-10 (IP-10 or CXCL10) in 32 episodes of neutropenia in 26 onco-hematological patients. IM were tested on enrollment and 24-48 h before the onset of fever and within 24 h of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutropenia; Cytokines; Oncological hematology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200200

In vivo and in vitro anti-inflammatory and anti-nociceptive activities of lovastatin in rodents
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Gonçalves,D.O.; Calou,I.B.F.; Siqueira,R.P.; Lopes,A.A.; Leal,L.K.A.; Brito,G.A.C.; Tomé,A.R.; Viana,G.S.B.
Statins are among the most prescribed drugs in recent clinical practice. They are also known for their pleiotropic actions, which are independent of their lipid-lowering properties. The effect of lovastatin was investigated against carrageenan-induced paw edema in male Wistar rats (200-250 g) and on leukocyte migration, as measured by carrageenan-induced peritonitis in male Swiss mice (20-25 g), which are models of acute inflammation. Lovastatin (administered 1 h prior to carrageenan), at oral doses of 2, 5, and 10 mg/kg, markedly attenuated paw edema formation in rats at the 4th hour after carrageenan injection (25, 43, and 37% inhibition, respectively). Inhibitions of 20, 45 and 80% were observed in the leukocyte migration, as evaluated by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Statin; Inflammation; Anti-nociception; Myeloperoxidase; Cytokines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200011

Oxidative stress and cellular immunity in patients with recurrent aphthous ulcers
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Avci,E.; Akarslan,Z.Z.; Erten,H.; Coskun-Cevher,S.
Recurrent aphthous ulcer (RAU) is an inflammatory condition of the oral mucosa characterized by painful, well-circumscribed, single or multiple round or ovoid ulcerations. The exact etiologic factor(s) of these ulcerations are not yet understood. The objective of this study was to evaluate inflammatory processes and free radical metabolism of 25 patients with RAUs compared to 25 healthy controls. The levels of malondialdehyde (MDA) and glutathione (GSH) were determined by high-performance liquid chromatography. Tumor necrosis factor-alpha (TNF-α), interleukin-2 (IL-2), IL-10, and IL-12 were determined by ELISA. Nitric oxide (NO), myeloperoxidase (MPO), total antioxidant status (TAS), and total oxidant status (TOS) levels were measured spectroscopically in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recurrent aphthous ulcer; Oxidative stress; Cytokines.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500355

Immunopathology of the duodenal mucosa of HIV-positive patients during combined antiretroviral therapy
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Machado,F.R.; Pagliari,C.; Caiafa,H.; Tapajós,R.; Duarte,M.I.S.
The objective of the present study was to evaluate the duodenal mucosa of HIV-infected patients during antiretroviral therapy. This was an observational study conducted on HIV-positive patients and a control group. Group 1 comprised 22 HIV-negative individuals while 38 HIV-positive individuals were classified according to the CDC 1993 classification into group 2 (A1 or A2) or group 3 (B2, A3, B3, C2, C3). All subjects were submitted to upper gastrointestinal endoscopy with duodenal biopsies. Qualitative, semi-quantitative and quantitative histological analyses were performed. Results were considered significant when P &lt; 0.05. A higher prevalence of inflammatory infiltrate and eosinophilia was observed in the HIV group, together with a reduction in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal histopathology; Cytokines; Human immunodeficiency virus; Acquired immunodeficiency syndrome; Antiretroviral therapy; CD4+.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100013

Understanding the mechanisms of lung mechanical stress
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Garcia,C.S.N.B.; Prota,L.F.M.; Morales,M.M.; Romero,P.V.; Zin,W.A.; Rocco,P.R.M.
Physical forces affect both the function and phenotype of cells in the lung. Bronchial, alveolar, and other parenchymal cells, as well as fibroblasts and macrophages, are normally subjected to a variety of passive and active mechanical forces associated with lung inflation and vascular perfusion as a result of the dynamic nature of lung function. These forces include changes in stress (force per unit area) or strain (any forced change in length in relation to the initial length) and shear stress (the stress component parallel to a given surface). The responses of cells to mechanical forces are the result of the cell's ability to sense and transduce these stimuli into intracellular signaling pathways able to communicate the information to its interior. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mechanical forces; Mechanosensors; Mechanotransduction; Cell-cell interactions; Cytokines; Extracellular matrix.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600001

Kinetics of TNF-alpha and IFN-gamma mRNA expression in islets and spleen of NOD mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ventura-Oliveira,D.; Vilella,C.A.; Zanin,M.E.; Castro,G.M.; Moreira Filho,D.C.; Zollner,R.L.
Insulin-dependent diabetes mellitus is caused by autoimmune destruction of pancreatic ß cells. Non-obese diabetic (NOD) mice spontaneously develop diabetes similar to the human disease. Cytokines produced by islet-infiltrating mononuclear cells may be directly cytotoxic and can be involved in islet destruction coordinated by CD4+ and CD8+ cells. We utilized a semiquantitative RT-PCR assay to analyze in vitro the mRNA expression of TNF-alpha and IFN-gamma cytokine genes in isolated islets (N = 100) and spleen cells (5 x 10(5) cells) from female NOD mice during the development of diabetes and from female CBA-j mice as a related control strain that does not develop diabetes. Cytokine mRNAs were measured at 2, 4, 8, 14 and 28 weeks of age from the onset of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; NOD mice; Cytokines; RT-PCR; Pancreatic islets; Time course.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100013

Early and late neuropathological features of meningoencephalitis associated with Maraba virus infection
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Maia-Farias,A.; Lima,C.M.; Freitas,P.S.L.; Diniz,D.G.; Rodrigues,A.P.D.; Quaresma,J.A.S.; Diniz,C.W. Picanço; Diniz,J.A.
Maraba virus is a member of the genus Vesiculovirus of the Rhabdoviridae family that was isolated in 1983 from sandflies captured in the municipality of Maraba, state of Pará, Amazônia, Brazil. Despite 30 years having passed since its isolation, little is known about the neuropathology induced by the Maraba virus. Accordingly, in this study the histopathological features, inflammatory glial changes, cytokine concentrations, and nitric oxide activity in the encephalon of adult mice subjected to Maraba virus nostril infection were evaluated. The results showed that 6 days after intranasal inoculation, severe neuropathological-associated disease signs appeared, including edema, necrosis and pyknosis of neurons, generalized congestion of encephalic vessels,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maraba virus; Vesiculovirus; Microglia; Astrocytes; Cytokines; Nitric oxide.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400610

Thalidomide protects mice against LPS-induced shock
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Moreira,A.L.; Wang,J.; Sarno,E.N.; Kaplan,G.
Thalidomide has been shown to selectively inhibit TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; production in vitro by lipopolysaccharide (LPS)-stimulated monocytes. TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; has been shown to play a pivotal role in the pathophysiology of endotoxic shock. Using a mouse model of LPS-induced shock, we investigated the effects of thalidomide on the production of TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; and other cytokines and on animal survival. After injection of 100-350 µg LPS into mice, cytokines including TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;, IL-6, IL-10, IL-1ß, GM-CSF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endotoxic shock; Thalidomide; Cytokines; Inflammation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000010

Fever induction pathways: evidence from responses to systemic or local cytokine formation
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Roth,J.; de Souza,G.E.P.
The immune and central nervous systems are functionally connected and interacting. The concept that the immune signaling to the brain which induces fever during infection and inflammation is mediated by circulating cytokines has been traditionally accepted. Administration of bacterial lipopolysaccharide (LPS) induces the appearance of a so-termed "cytokine cascade" in the circulation more or less concomitantly to the developing febrile response. Also, LPS-like fever can be induced by systemic administration of key cytokines (IL-1ß, TNF-alpha, and others). However, anti-cytokine strategies against IL-1ß or TNF-alpha along with systemic injections of LPS frequently lead to attenuation of the later stages of the febrile response but not of the initial phase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fever; Cytokines; Neuroimmunomodulation; Humoral factors; Afferent nerves; Central nervous system.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300003

Serum cytokines in patients with Brazilian pemphigus foliaceus (fogo selvagem)
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zeoti,D.M.; Figueiredo,J.F.C.; Chiossi,M.P.V.; Roselino,A.M.F.
Pemphigus foliaceus (PF) is characterized by acantholysis determined by IgG4 binding to desmoglein I, a 160-kDa desmosomal glycoprotein. To investigate the immunopathological aspects of Brazilian PF, we determined levels of serum cytokines in patients with PF. Twenty-five patients with PF and a control group consisting of 10 healthy individuals were studied. Serum IL-2, IL-4, IL-5, IL-10, IL-12 and IFN-gamma were measured in the two groups by ELISA. The median concentration of IL-2 was lower in PF patients compared to the control group (0.45 and 9.50 pg/ml, respectively), as also was the concentration of IL-4 (0.26 and 10.16 pg/ml, respectively). The same was observed for IL-5 (7.94 and 15.74 pg/ml, respectively) and for IFN-gamma (5.90 and 8.58 pg/ml,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Pemphigus; Pemphigus foliaceus; Fogo selvagem; Bullous dermatoses.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900012

Immune cells and oxidative stress in the endotoxin tolerance mouse model
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Melo,E.S.; Barbeiro,H.V.; Ariga,S.; Goloubkova,T.; Curi,R.; Velasco,I.T.; Vasconcelos,D.; Soriano,F.G.
Sepsis is a systemic inflammatory response that can lead to tissue damage and death. In order to increase our understanding of sepsis, experimental models are needed that produce relevant immune and inflammatory responses during a septic event. We describe a lipopolysaccharide tolerance mouse model to characterize the cellular and molecular alterations of immune cells during sepsis. The model presents a typical lipopolysaccharide tolerance pattern in which tolerance is related to decreased production and secretion of cytokines after a subsequent exposure to a lethal dose of lipopolysaccharide. The initial lipopolysaccharide exposure also altered the expression patterns of cytokines and was followed by an 8- and a 1.5-fold increase in the T helper 1 and 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sepsis; Cytokines; Immune tolerance; Lipopolysaccharide; Murine sepsis model; T helper cells.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100009

Possible in vivo mechanisms involved in photodynamic therapy using tetrapyrrolic macrocycles
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Filip,A.G.; Clichici,S.; Daicoviciu,D.; Ion,R.M.; Tatomir,C.; Rogojan,L.; Opris,I.; Mocan,T.; Olteanu,D.; Muresan,A.
Photodynamic therapy (PDT) mediated by oxidative stress causes direct tumor cell damage as well as microvascular injury. To improve this treatment new photosensitizers are being synthesized and tested. We evaluated the effects of PDT with 5,10,15,20-tetrakis(4-methoxyphenyl)-porphyrin (TMPP) and its zinc complex (ZnTMPP) on tumor levels of malondialdehyde (MDA), reduced glutathione (GSH) and cytokines, and on the activity of caspase-3 and metalloproteases (MMP-2 and -9) and attempted to correlate them with the histological alterations of tumors in 3-month-old male Wistar rats, 180 ± 20 g, bearing Walker 256 carcinosarcoma. Rats were randomly divided into five groups: group 1, ZnTMPP+irradiation (IR) 10 mg/kg body weight; group 2, TMPP+IR 10 mg/kg body...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic therapy; Oxidative stress; Apoptosis; Tetrapyrrolic macrocycles; Cytokines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100008

Amifostine reduces inflammation and protects against 5-fluorouracil-induced oral mucositis and hyposalivation
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Barbosa,S.C.M.; Pereira,V.B.M.; Wong,D.V.T.; Santana,A.P.M.; Lucetti,L.T.; Carvalho,L.L.; Barbosa,C.R.N.; Callado,R.B.; Silva,C.A.A.; Lopes,C.D.H.; Brito,G.A.C.; Alencar,N.M.N.; Lima-Júnior,R.C.P.
Oral mucositis (OM) is a common and dose-limiting side effect of cancer treatment, including 5-fluorouracil (5-FU) and radiotherapy. The efficacy of the therapeutic measures to prevent OM is limited and disease prevention is not fully observable. Amifostine is a cytoprotective agent with a described anti-inflammatory potential. It is clinically used to reduce radiotherapy and chemotherapy-associated xerostomia. This study investigated the protective effect of amifostine on an experimental model of OM. Hamsters were divided into six groups: saline control group (5 mL/kg), mechanical trauma (scratches) of the right cheek pouch; 5-FU (60 and 40 mg/kg, ip, respectively, administered on days 1 and 2); amifostine (12.5, 25, or 50 mg/kg) + 5-FU + scratches....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer chemotherapy toxicity; Oral mucositis; 5-Fluorouracil; Amifostine; Cytokines.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300601

Cytokine profile and pathology in human leishmaniasis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ribeiro-de-Jesus,A.; Almeida,R.P.; Lessa,H.; Bacellar,O.; Carvalho,E.M.
The clinical spectrum of leishmaniasis and control of the infection are influenced by the parasite-host relationship. The role of cellular immune responses of the Th1 type in the protection against disease in experimental and human leishmaniasis is well established. In humans, production of IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; is associated with the control of infection in children infected by Leishmania chagasi. In visceral leishmaniasis, an impairment in IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; production and high IL-4 and IL-10 levels (Th2 cytokines) are observed in antigen-stimulated peripheral blood mononuclear cells (PBMC). Moreover, IL-12 restores IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; production and enhances the cytotoxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Human leishmaniasis; Cytokines; Pathology; Immunological responses.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100020

Aging reduces the primary humoral response and the in vitro cytokine production in mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Simioni,P.U.; Costa,E.H.; Tamashiro,W.M.S.C.
Aging is accompanied by a decrease in several physiological functions that make older individuals less responsive to environmental challenges. In the present study, we analyzed the immune response of female BALB/c mice (N = 6) of different ages (from 2 to 96 weeks) and identified significant age-related alterations. Immunization with hapten-protein (trinitrophenyl-bovine serum albumin) conjugates resulted in lower antibody levels in the primary and secondary responses of old mice (72 weeks old). Moreover, young mice (2, 16, and 32 weeks old) maintained specific antibodies in their sera for longer periods after primary immunization than did old mice. However, a secondary challenge efficiently induced memory in old mice, as shown by the increased antibody...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Cytokines; Antibody; Proliferation; T cell response; T cell subsets.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800012

Autoimmune diseases in the TH17 era
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Mesquita Jr.,D.; Cruvinel,W.M.; Câmara,N.O.S.; Kállas,E.G.; Andrade,L.E.C.
A new subtype of CD4+ T lymphocytes characterized by the production of interleukin 17, i.e., TH17 cells, has been recently described. This novel T cell subset is distinct from type 1 and type 2 T helper cells. The major feature of this subpopulation is to generate significant amounts of pro-inflammatory cytokines, therefore appearing to be critically involved in protection against infection caused by extracellular microorganisms, and in the pathogenesis of autoimmune diseases and allergy. The dynamic balance among subsets of T cells is important for the modulation of several steps of the immune response. Disturbances in this balance may cause a shift from normal immunologic physiology to the development of immune-mediated disorders. In autoimmune diseases,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autoimmune diseases; Cytokines; TH17 cells; IL-17; IL-22; IL-23.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600002

The neuroimmune-endocrine axis: pathophysiological implications for the central nervous system cytokines and hypothalamus-pituitary-adrenal hormone dynamics
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Licinio,J.; Frost,P.
Cytokines are molecules that were initially discovered in the immune system as mediators of communication between various types of immune cells. However, it soon became evident that cytokines exert profound effects on key functions of the central nervous system, such as food intake, fever, neuroendocrine regulation, long-term potentiation, and behavior. In the 80's and 90's our group and others discovered that the genes encoding various cytokines and their receptors are expressed in vascular, glial, and neuronal structures of the adult brain. Most cytokines act through cell surface receptors that have one transmembrane domain and which transduce a signal through the JAK/STAT pathway. Of particular physiological and pathophysiological relevance is the fact...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central nervous system; Cytokines; Immune system; Neuroendocrine regulation; Fever; Food intake.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000003

Immunity and immunosuppression in experimental visceral leishmaniasis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Goto,H.; Lindoso,J.A.L.
Leishmaniasis is a disease caused by protozoa of the genus Leishmania, and visceral leishmaniasis is a form in which the inner organs are affected. Since knowledge about immunity in experimental visceral leishmaniasis is poor, we present here a review on immunity and immunosuppression in experimental visceral leishmaniasis in mouse and hamster models. We show the complexity of the mechanisms involved and differences when compared with the cutaneous form of leishmaniasis. Resistance in visceral leishmaniasis involves both CD4+ and CD8+ T cells, and interleukin (IL)-2, interferon (IFN)- gamma, and IL-12, the latter in a mechanism independent of IFN- gamma and linked to transforming growth factor (TGF)-ß production. Susceptibility involves IL-10 but not IL-4,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Immunosuppression; T cell; Cytokines; Mice; Hamster.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400020

Morphometric evaluation of nitric oxide synthase isoforms and their cytokine regulators predict pulmonary dysfunction and survival in systemic sclerosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Parra,E.R.; Aguiar Junior,A.C.; Silva,L.O.; Souza,H.S.P.; Espinoza,J.D.; Capelozzi,V.L.
Because histopathological changes in the lungs of patients with systemic sclerosis (SSc) are consistent with alveolar and vessel cell damage, we presume that this interaction can be characterized by analyzing the expression of proteins regulating nitric oxide (NO) and plasminogen activator inhibitor-1 (PAI-1) synthesis. To validate the importance of alveolar-vascular interactions and to explore the quantitative relationship between these factors and other clinical data, we studied these markers in 23 cases of SSc nonspecific interstitial pneumonia (SSc-NSIP). We used immunohistochemistry and morphometry to evaluate the amount of cells in alveolar septa and vessels staining for NO synthase (NOS) and PAI-1, and the outcomes of our study were cellular and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic sclerosis; Nitric oxide synthase isoforms; Cytokines; Morphometry; Pulmonary function tests and survival.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000881

Immune factors and immunoregulation in tuberculosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ferraz,J.C.; Melo,F.B.S.; Albuquerque,M.F.P.M.; Montenegro,S.M.L.; Abath,F.G.C.
Pathogens causing tuberculosis and other chronic infectious diseases of major public health importance commonly have complex mechanisms involved in their persistence in the host despite specific and sometimes strong immune responses. These diseases are also associated with the lack of efficient vaccines, difficult therapeutics and a high mortality rate among susceptible individuals. Here, we will review features of the host immune response that contribute to the occurrence of disease. In addition, we propose that the immune responses observed in tuberculosis cannot be interpreted solely on the basis of a Th1-Th2 counter-regulatory paradigm since there is growing evidence that natural regulatory T cells may play an important role in the regulation of host...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Mycobacteria; Regulatory T cells; Cytokines.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100002

Decreased muscle strength is associated with proinflammatory cytokines but not testosterone levels in men with diabetes
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ferreira,J.P.; Leal,A.M.O.; Vasilceac,F.A.; Sartor,C.D.; Sacco,I.C.N.; Soares,A.S.; Salvini,T.F.
The aim of this study was to compare muscle strength in male subjects with type 2 diabetes mellitus (DM2) with and without low plasma testosterone levels and assess the relationship between muscle strength, testosterone levels, and proinflammatory cytokines. Males (75) aged between 18 and 65 years were divided into 3 groups: control group that did not have diabetes and had a normal testosterone plasma level (&gt;250 ng/dL), DnormalTT group that had DM2 with normal testosterone levels, and the DlowTT group that had DM2 and low plasma testosterone levels (&lt;250 ng/dL). The age (means±SD) of the groups was 48.4±10, 52.6±7, and 54.6±7 years, respectively. Isokinetic concentric and isometric torque of knee flexors and extensors were analyzed by an isokinetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Testosterone; Muscle strength; Cytokines.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900610

The role of T cell subsets and cytokines in the regulation of intracellular bacterial infection
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Oliveira,S.C.; Harms,J.S.; Rech,E.L.; Rodarte,R.S.; Bocca,A.L.; Goes,A.M.; Splitter,G.A.
Cellular immune responses are a critical part of the host's defense against intracellular bacterial infections. Immunity to Brucella abortus crucially depends on antigen-specific T cell-mediated activation of macrophages, which are the major effectors of cell-mediated killing of this organism. T lymphocytes that proliferate in response to B. abortus were characterized for phenotype and cytokine activity. Human, murine, and bovine T lymphocytes exhibited a type 1 cytokine profile, suggesting an analogous immune response in these different hosts. In vivo protection afforded by a particular cell type is dependent on the antigen presented and the mechanism of antigen presentation. Studies using MHC class I and class II knockout mice infected with B. abortus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: T cell subsets; Brucella abortus; Knockout mice; Cytokines; Intracellular pathogens; Genetic immunization.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100010

Expression and cytokine secretion in the states of immune reactivation in leprosy
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Sampaio,E.P.; Sarno,E.N.
Leprosy is a chronic inflammatory disease caused by Mycobacterium leprae. The human response to this pathogen exhibits intriguing aspects which are up to now not well understood. The present study discusses the probable mechanisms involved in T cell-specific unresponsiveness observed in lepromatous patients. Analysis of the cytokine profile either in blood leukocytes or in skin specimens taken from leprosy lesions indicates that some parameters of Th1 immune response are present in lepromatous patients under reactional states
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eprosy; Reactions; Cytokines.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100009

Th-1 and Th-2 cytokine production in infants with virus-associated wheezing
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pitrez,P.M.C.; Machado,D.C.; Jones,M.H.; Andrade,F.; Camozzato,C.; Stein,R.T.
Wheezing associated with respiratory viral infections in infancy is very common and results in high morbidity worldwide. The Th1/Th2 pattern of immune response in these patients remains unclear and previous studies have shown controversial results. The aim of the present study was to compare the type of Th1/Th2 cytokine response between infants with acute bronchiolitis, recurrent wheezing and upper respiratory infections from a developing country. Infants younger than 2 years of age admitted to Hospital São Lucas, Porto Alegre, RS, Brazil, between May and November 2001, with an acute episode of wheezing associated with viral respiratory infection were selected. Subjects with upper respiratory infections from the emergency department were selected for the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Acute bronchiolitis; Inflammation; Interferon-gamma; Interleukin-4; Cytokines.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100008

Cytokines produced by susceptible and resistant mice in the course of Paracoccidioides brasiliensis infection
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Calich,V.L.G.; Kashino,S.S.
Paracoccidioidomycosis (PCM) is the most prevalent deep mycosis in Latin America and presents a wide spectrum of clinical manifestations. We established a genetically controlled murine model of PCM, where A/Sn mice develop an infection which mimics the benign disease (immune responses which favor cellular immunity) and B10.A animals present the progressive disseminated form of PCM (preferential activation of B cells and impairment of cellular immune responses). To understand the immunoregulatory phenomena associated with resistance and susceptibility in experimental PCM, A/Sn and B10.A mice were studied regarding antigen-elicited secretion of monokines (TNF-&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; and TGF-ß) and type-1 (IL-2 and IFN-&lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Murine paracoccidioidomycosis; Genetic resistance; Cytokines; Type-1 cytokines; Type-2 cytokines; Monokines.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500003

Role of Th1 and Th2 cells in autoimmune demyelinating disease
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Nagelkerken,L.
Evidence is accumulating that Th1 cells play an important role in the development of multiple sclerosis (MS) and experimental allergic encephalomyelitis (EAE), whereas Th2 cells contribute to recovery from disease. A major determinant in the development of Th1 and Th2 cells is the type of antigen-presenting cell (APC) involved and its functional characteristics, e.g., the production of interleukin-12. Therefore, modulation of APC might interfere with the development of Th1 type responses and as such be beneficial for MS and EAE. The potential of cytokines, in particular interleukin-10, and glucocorticoids to exert a selective effect on APC, and as a consequence to affect the Th1-Th2 balance in EAE, is discussed
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiple sclerosis; Experimental allergic encephalomyelitis; Cytokines; Glucocorticoids; Th1 cells; Antigen-presenting cells.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100007

Blastocyst-endometrium interaction: intertwining a cytokine network
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Castro-Rendón,W.A.; Castro-Álvarez,J.F.; Guzmán-Martinez,C.; Bueno-Sanchez,J.C.
The successful implantation of the blastocyst depends on adequate interactions between the embryo and the uterus. The development of the embryo begins with the fertilized ovum, a single totipotent cell which undergoes mitosis and gives rise to a multicellular structure named blastocyst. At the same time, increasing concentrations of ovarian steroid hormones initiate a complex signaling cascade that stimulates the differentiation of endometrial stromal cells to decidual cells, preparing the uterus to lodge the embryo. Studies in humans and in other mammals have shown that cytokines and growth factors are produced by the pre-implantation embryo and cells of the reproductive tract; however, the interactions between these factors that converge for successful...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo implantation; Trophoblast; Cytokines; Prostaglandins; Aspirin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100001

Induction of cell-mediated immunity during early stages of infection with intracellular protozoa
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Gazzinelli,R.T.; Talvani,A.; Camargo,M.M.; Santiago,H.C.; Oliveira,M.A.P.; Vieira,L.Q.; Martins,G.A.; Aliberti,J.C.S.; Silva,J.S.
Toxoplasma gondii and Trypanosoma cruzi are intracellular parasites which, as part of their life cycle, induce a potent cell-mediated immunity (CMI) maintained by Th1 lymphocytes and IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;. In both cases, induction of a strong CMI is thought to protect the host against rapid parasite multiplication and consequent pathology and lethality during the acute phase of infection. However, the parasitic infection is not eliminated by the immune system and the vertebrate host serves as a parasite reservoir. In contrast, Leishmania sp, which is a slow growing parasite, appears to evade induction of CMI during early stages of infection as a strategy for surviving in a hostile environment (i.e., inside the macrophages which are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Toxoplasma gondii; Trypanosoma cruzi; Macrophages; Cytokines; Nitric oxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100012

Cytokine profile in childhood-onset systemic lupus erythematosus: a cross-sectional and longitudinal study
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cavalcanti,A.; Santos,R.; Mesquita,Z.; Duarte,A.L.B.P.; Lucena-Silva,N.
Childhood-onset systemic lupus erythematosus (cSLE) exhibits an aggressive clinical phenotype and severe complications. This could be due to a pro-inflammatory cytokine milieu. Therefore, we determined plasma levels of Th1 (IL-2, IFN-γ, TNF), Th2 (IL-4), Th17 (IL-17A, IL-6), and Treg (IL-10) cytokines in a cohort of cSLE patients and healthy controls, and we evaluated the association between these cytokines and disease activity. We conducted a cross-sectional study with 51 cSLE patients from two pediatric rheumatology services. Ten cSLE patients participated in a longitudinal follow-up study. Blood samples were collected from the same patient during active and inactive disease. Disease activity was evaluated according to SLE Disease Activity Index 2000...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Childhood-onset systemic lupus erythematosus; Disease activity; SLEDAI-2K; Inflammation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400704

Aging alters the production of iNOS, arginase and cytokines in murine macrophages
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cecílio,C.A.; Costa,E.H.; Simioni,P.U.; Gabriel,D.L.; Tamashiro,W.M.S.C.
The limited amount of information on the primary age-related deficiencies in the innate immune system led us to study the production of inducible nitric oxide synthase (iNOS), arginase, and cytokines in macrophages of young (8 weeks old) and old (72 weeks old) female BALB/c mice. We first evaluated iNOS and arginase inducers on peritoneal (PMΦ) and bone marrow-derived (BMMΦ) macrophages of young BALB/c and C57BL/6 mice, and then investigated their effects on macrophages of old mice. Upon stimulation with lipopolysaccharide (LPS), resident and thioglycolate-elicited PMΦ from young mice presented higher iNOS activity than those from old mice (54.4%). However, LPS-stimulated BMMΦ from old mice showed the highest NO levels (50.1%). Identical NO levels were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; INOS; Arginase; Cytokines; Murine macrophages; Inflammatory stimuli.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700010

Interleukin-5 and interleukin-10 are major cytokines in cerebrospinal fluid from patients with active neurocysticercosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Rodrigues Jr.,V.; de-Mello,F.A.; Magalhães,E.P.; Ribeiro,S.B.F.; Marquez,J.O.
Neurocysticercosis (NCC) is a common neurological disorder especially in developing countries, caused by infection of the brain with encysted larvae of the tapeworm Taenia solium. Seizures are a common finding associated with this disease. The objective of the present study was to evaluate the correlation between the levels of various cytokines present in the cerebrospinal fluid (CSF) of patients with NCC and the severity of the disease. The levels of the cytokines IL-1ß, TNF-alpha, IL-5, IL-10 and IFN-gamma were determined in the CSF of 22 patients with active NCC, 13 patients with inactive NCC and 15 control subjects. CSF from patients with active NCC presented significantly higher IL-5 levels compared to control subjects. IL-5 and IL-10 levels in CSF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cysticercosis; Cytokines; Cerebrospinal fluid.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900011

The role of CD8+ T cells during allograft rejection
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bueno,V.; Pestana,J.O.M.
Organ transplantation can be considered as replacement therapy for patients with end-stage organ failure. The percent of one-year allograft survival has increased due, among other factors, to a better understanding of the rejection process and new immunosuppressive drugs. Immunosuppressive therapy used in transplantation prevents activation and proliferation of alloreactive T lymphocytes, although not fully preventing chronic rejection. Recognition by recipient T cells of alloantigens expressed by donor tissues initiates immune destruction of allogeneic transplants. However, there is controversy concerning the relative contribution of CD4+ and CD8+ T cells to allograft rejection. Some animal models indicate that there is an absolute requirement for CD4+ T...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transplantation; Rejection; T cells; Cytokines; Chemokines.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100001

Lactobacillus delbrueckii UFV-H2b20 induces type 1 cytokine production by mouse cells in vitro and in vivo
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Neumann,E.; Ramos,M.G.; Santos,L.M.; Rodrigues,A.C.P.; Vieira,E.C.; Afonso,L.C.C.; Nicoli,J.R.; Vieira,L.Q.
Lactobacillus delbrueckii UFV-H2b20 has been shown to increase clearance of bacteria injected into the blood of germ-free mice. Moreover, it induces the production of type 1 cytokines by human peripheral mononuclear cells. The objective of the present study was to investigate the production of inflammatory cytokines [interleukin-12 (IL-12 p40), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ)] triggered in vitro by live, heat-killed or lysozyme-treated L. delbrueckii UFV-H2b20 and in vivo by a live preparation. Germ-free, L. delbrueckii-monoassociated and lipopolysaccharide (LPS)-resistant C3H/HeJ mice were used as experimental models. UFV-H2b20 induced the production of IL-12 p40 and TNF-α by peritoneal cells and IFN-γ by spleen cells from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Probiotics; Cytokines; Macrophages; IFN-γ; IL-12; Lactobacillus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400008

Cytokine accumulation in osteitis fibrosa of renal osteodystrophy
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Duarte,M.E.L.; Carvalho,E.F.; Cruz,E.A.S.; Lucena,S.B.G.; Andress,D.L.
Bone marrow fibrosis occurs in association with a number of pathological states. Despite the extensive fibrosis that sometimes characterizes renal osteodystrophy, little is known about the factors that contribute to marrow accumulation of fibrous tissue. Because circulating cytokines are elevated in uremia, possibly in response to elevated parathyroid hormone levels, we have examined bone biopsies from 21 patients with end-stage renal disease and secondary hyperparathyroidism. Bone sections were stained with antibodies to human interleukin-1alpha (IL-1alpha), IL-6, IL-11, tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-ß (TGF-ß) using an undecalcified plastic embedding method. Intense staining for IL-1alpha, IL-6, TNF-alpha and TGF-ß...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Osteitis fibrosa; Renal osteodystrophy; Chronic renal failure; Dialysis; Secondary hyperparathyroidism.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100004

Conditioned medium from activated spleen cells supports the survival of rat retinal cells in vitro
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Sholl-Franco,A.; Araujo,E.G.
Cytokines are a heterogeneous group of molecules that have been associated with several functions in the nervous system, such as survival and differentiation of neuronal and glial cells. In the present study, we demonstrated that conditioned medium from spleen cells activated with concanavalin A increased neuritogenesis and survival of retinal cells, as measured by biochemical and morphological criteria. Our data showed that conditioned medium induced a five-fold increase in the amount of protein after 120 h in vitro. This effect was not inhibited by the blockade of voltage-dependent L-type calcium channels with 5.0 µM nifedipine. However, the use of an intracellular calcium chelator (15.0 µM BAPTA-AM) inhibited this effect. Our results support the idea...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Growth factor; Neuroimmunology; Retina; Development.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100006

Acute effect of passive cycle-ergometry and functional electrical stimulation on nitrosative stress and inflammatory cytokines in mechanically ventilated critically ill patients: a randomized controlled trial
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: França,E.E.T.; Gomes,J.P.V.; De Lira,J.M.B.; Amaral,T.C.N.; Vilaça,A.F.; Paiva Júnior,M.D.S.; Elihimas Júnior,U.F.; Correia Júnior,M.A.V.; Forgiarini Júnior,L.A.; Costa,M.J.C.; Andrade,M.A.; Ribeiro,L.C.; De Castro,C.M.M.B.
Early mobilization is beneficial for critically ill patients because it reduces muscle weakness acquired in intensive care units. The objective of this study was to assess the effect of functional electrical stimulation (FES) and passive cycle ergometry (PCE) on the nitrous stress and inflammatory cytometry in critically ill patients. This was a controlled, randomized, open clinical trial carried out in a 16-bed intensive care unit. The patients were randomized into four groups: Control group (n=10), did not undergo any therapeutic intervention during the study; PCE group (n=9), lower-limb PCE for 30 cycles/min for 20 min; FES group (n=9), electrical stimulation of quadriceps muscle for 20 min; and FES with PCE group (n=7), patients underwent PCE and FES,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Nitrosative stress; Intensive care unit; Physiotherapy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400605

Production of reactive oxygen (H2O2) and nitrogen (NO) intermediates and TNF-α in mice genetically selected for high (H) and low (L) antibody response and experimentally infected with Leptospira serovar pomona
Provedor de dados: Brazilian Journal of Microbiology
Autores: Haanwinckel,Maria Cristina Santos; Oliveira,Silvio Luis de.
The aim of the present study was to evaluate the activity of macrophages, and the production of TNF-α and antibodies against experimental infection by Leptospira serovar Pomona in mice genetically selected for High (H) or Low (L) humoral immune response. To evaluate macrophagic activity, peritoneal and splenic lavages were performed for determination of oxygen (H2O2) and nitrogen (NO) intermediates. The production of the tumor necrosis factor (TNF-α) was investigated through bioassays in serum and homogenates of splenic and hepatic cells of control and infected animals, as was as specific antibodies production. The immune response against serovar Pomona in those lines, was characterized by high antibody production, especially in later periods of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunological aspects; Biozzi mice; Leptospira; Macrophages; Cytokines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200041

Influence of isoniazid on T lymphocytes, cytokines, and macrophages in rats
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Cao,Bianchuan; Li,Qiuju; Huang,Zhe; Huang,Xiufang; Zhu,Yihong; Xiao,Ke; Huang,Fuli; Zhong,Li.
T lymphocytes, cytokines, and macrophages play important roles in the clearance of Mycobacterium tuberculosis (Mtb) by the immune system. This study aimed to investigate the effects of isoniazid on the functions of both innate and adaptive immune cells. Healthy rats were randomly divided into experimental and control groups. Each group was randomly divided into three subgroups and named according to the duration of drug feeding, 1, 3, and 3 months followed by drug withdrawal for 1 month. The experimental groups were fed with isoniazid (12 mg/mL) and the control groups with normal saline. The percentage of CD4+ and CD8+T lymphocytes, level of interleukin (IL)-12 and interferon (IFN)-γ, and function of macrophages were determined at these three time points....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Immune; Isoniazid; Macrophages; T lymphocytes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100573

Astrocytic expression of GFAP and serum levels of IL-1β and TNF-α in rats treated with different pain relievers
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Amaral,Gisele Ferreira; Dossa,Pietro Domingues; Viebig,Lígia Bocamino; Konno,Fabiana Toshie Camargo; Consoli,Amanda; Martins,Maria de Fátima Monteiro; Viani,Flávio Cesar; Bondan,Eduardo Fernandes.
ABSTRACT Pro-inflammatory cytokines and glial cells, especially microglial cells, have been implicated in persistent pain sensitization. Less is known about the role of astrocytes in pain regulation. This study aimed to observe the expression of the astrocytic biomarker glial fibrillary acidic protein (GFAP) and the serum levels of interleukin 1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) after short-term administration of central pain relievers in rats not submitted to noxious stimuli. Male Wistar rats were divided into five groups, receiving for nine days- (1) amitriptyline (Amt-10 mg/kg/day, by gavage); (2) gabapentin (Gb-60 mg/kg/day, by gavage; (3) methadone (Me-4.5 mg/kg/day, intraperitoneal route [IP]); (4) morphine (Mo-10 mg/kg/day, IP);...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuropathic pain/treatment; Astrocytes/pain regulation; Analgesics/tests/treatment; Cytokines; Glial Fibrillary Acidic Protein/study.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400623

Effect of acupuncture on TNF-alpha, IL-1b and IL-10 concentrations in the peritoneal exudates of carrageenan-induced peritonitis in rats
Provedor de dados: Ciência Rural
Autores: Scognamillo-Szabó,Márcia Valéria Rizzo; Bechara,Gervásio Henrique; Cunha,Fernando de Queiroz.
Acupuncture is an ancient and empirical therapeutic procedure known by its efficacy in the treatment of pain. However, the influence of acupuncture on inflammatory process is still poorly understood and additional research is needed. In this work, we investigated the mechanism of action of manual acupuncture on the inhibition of neutrophil migration to the peritoneal cavity induced by the inflammatory stimulus carrageenan in Wistar rats. Previous results from our laboratory showed that this anti-inflammatory effect is not due to endogenous corticoid release. Furthermore, the concentration of IL-&lt;FONT FACE=Symbol&gt;1b&lt;/FONT&gt;, but not of TNF-alpha or IL-10 in the carrageenan-induced exudates was reduced in the acupuncture group. Further research...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acupuncture; Inflammation; Cytokines; Neutrophils; Rats; Carrageenan.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000100016