Consulta: subjectFacets:"Detection"
Registros recuperados: 57
Data/hora: 13/06/2024 00:44:47

PCR-based sensitive detection of the edible fungus Boletus edulis from rDNA ITS sequences
Provedor de dados: Electronic Journal of Biotechnology
Autores: Lian,Bin; Zang,Jin-ping; Hou,Wei-guo; Yuan,Sheng; Smith,Donald L.
Identification of commercially important fungi, such as the valuable edible fungus Boletus edulis can be difficult considering visual or metabolic approaches. Based on phylogenetic analysis of the rDNA ITS sequence, a pair of specific primers was designed for differentiating B. edulis from other mushrooms by PCR. PCR was performed with total DNA as a template at an annealing temperature between 56-60ºC. Positive amplicons were obtained from B. edulis with all DNA templates from fruit bodies and cultured mycelium, but not from other fungal species at an annealing temperature of 60ºC. The result indicated that B. edulis could be clearly distinguished from other fungi by PCR, and there were no misidentifications under the reaction conditions used. The primers...
Tipo: Journal article
Palavras-chave: Boletus edulis; Detection; Edible fungi; Internal transcribed spacer; PCR; Specific primers.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300011

Deteccao de virus em sementes.
Provedor de dados: Informação Tecnológica em Agricultura (Infoteca-e)
Autores: ALMEIDA, A. M. R.
Detecção de virus em sementes. Técnicas de detecção. Teste de observação visual.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Deteccao; Seed.; Semente; Quarentena; Vírus.; Detection; Quarantine.
Ano: 1999
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/460704

Rapid and sensitive detection of ostreid Herpesvirus 1 in oyster samples by real-time PCR (Poster)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Pepin, Jean-francois; Riou, Antoine; Renault, Tristan.
Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region, which is present in two copies in the OsHV-1 genome and encodes two proteins of unknown function. The assay was applied successfully to rapid diagnosis (100 minutes)...
Tipo: Text
Palavras-chave: Crassostrea gigas; PCR; Oyster; OsHV 1; Detection; Herpes virus 1.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3653.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - Final Report 4th January 1999 to 3rd January 2002
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Little information is available on viral infections that affect bivalve molluscs. Such a lack of data is due ta a certain inadequacy of the diagnosis methods that are employed when massive mortality events occur. Most laboratories involved in mollusc pathology still analyse samples through light microscopy. World-wide, there is thus currently a lack of information concerning the occurrence of bivalve herpesvituses. This is probably due to the lack of suitable diagnostic tools. The basic method for identification and examination of suspect samples is predominantly histopathology. This enables the identification of any cellular changes, but is not conclusive identification of bivalve herpesviruses. This technique doesn't allow, by itself, to detect viruses...
Tipo: Text
Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00044/15525/12912.pdf

Detection of ostreid herpesvirus 1 (OsHV-1) DNA in seawater by PCR: influence of water parameters in bioassays
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Vigneron, Vassilia; Solliec, Gaelle; Montanie, Hélène; Renault, Tristan.
Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted...
Tipo: Text
Palavras-chave: Temperature; PCR; Detection; Seawater; Viral DNA; Oyster herpesvirus 1.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2909.pdf

Molecular detection of betanodavirus from the farmed fish, Platax orbicularis (Forsskal) (Ephippidae), in French Polynesia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: David, R.; Treguier, Cathy; Montagnani, Caroline; Belliard, Corinne; Levy, Peva; Nedelec, Georges; Joufoques, Vaiana; Remoissenet, G.; Gueguen, Yannick; Cochennec-laureau, Nathalie.
Tipo: Text
Palavras-chave: Betanodavirus; Detection; French Polynesia; Genotype; Platax orbicularis.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00003/11380/7972.pdf

Assessment of biochemical methods to detect enzymatic depolymerization of polysaccharides
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Rigouin, Coraline; Delbarre Ladrat, Christine; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, M.
To find biocatalyst allowing depolymerization of new polysaccharides, one needs to get a sensitive and well adapted method to a screening program. This led us to compare biochemical methods of detection of the depolymerization. Currently used methods such as reducing sugars assays, double bond monitoring or molecular weight determination were tested to follow the kinetic of depolymerization with different enzyme / polysaccharide couples. The range of concentrations of assorted enzymes allowed us to identify the most sensitive and appropriate method to detect polysaccharide degradation. Reducing sugars assays are quantitative, sensitive and almost usable with all kind of polysaccharide but some compounds may interfere with them. When polysaccharide is...
Tipo: Text
Palavras-chave: Detection; Enzyme; Depolymerization; Carbohydrate.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2008/publication-4723.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - First Periodic Progress Report 4th January 1999 to 3rd January 2000
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using techniques developed by the partners to characterise viruses by studying their genomes and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpes-like virus DNA sequence with determination of the genome structure. 2 - Comparing oyster herpes-like viruses with other viruses belonging to the Helpesviridae family on the basis of sequence data and...
Tipo: Text
Palavras-chave: Pathology; Diagnosis; Oyster; Herpes like virus; Herpesviridae; Immunology; Detection; Virus; Bivalves.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00044/15523/12910.pdf

First record of Marteilia sp in mussels Mytilus galloprovincialis in Croatia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Zrncic, Snjevana; Le Roux, Frédérique; Oraic, Drazen; Sostaric, Branko; Berthe, Franck.
Marteiliosis is a disease of molluscs caused by Marteilia refringens in Europe and M. sydneyi in Australia. During routine examination of cultured mussels Mytilus galloprovinciallis in the northern Adriatic, the occurrence of Marteilia sp. was recorded with a prevalence of 5 %. This parasite was not detected in flat oysters reared in the same area. The affiliation of the detected parasite in M. galloprovinciallis was confirmed by in situ hybridization using a M. refringens probe, specific at the genus level. DNA of these infected mussels originating from the same area will be used to clarify the taxonomic position of this species within the genus Marteilia using a molecular approach.
Tipo: Text
Palavras-chave: Taxonomy; Detection; Mytilus galloprovincialis; Parasite; Marteilia maurini.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-817.pdf

Development of genomic probes for detection of hepatitis A virus and enteroviruses in shellfish
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Apaire, M; Leguyader, F; Besse, B; Billaudel, S.
The development of molecular biology techniques has modified research procedures for certain viruses, particularly hepatitis A virus (HAV) and enteroviruses. Accordingly, we designed cDNA and RNA genomic probes to detect viruses in shellfish in the environment. Better sensitivity was noted with RNA than cDNA probes: only 17/83 shellfish samples were positive with HAV and enterovirus DNA probes, where as 42/83 were positive with HAV and enterovirus RNA probes.
Tipo: Text
Palavras-chave: Mollusca; Enterovirus; Hepatitis A virus; Picornaviridae; RNA probes; DNA probes; Seafood; Biology; Sensors; DNA; RNA; Viruses; Detection; Marine molluscs; Shellfish.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1613.pdf

Development of a new diagnostic tool (Mini-Array) for ostreid Herpesvrus 1 (OsHV-1) detection in the Pacific oyster Crassostre gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Varsamos, S.; Pepin, Jean-francois; Sauvage, Christopher; Renault, Tristan.
OsHV-1 was the first herpesvirus to be identified in an invertebrate host and is associated to mortalities in the Pacific oyster (Crassostrea gigas) and other economically important bivalve species. Infections due to OsHV-1 are frequently reported and cause high mortality rates in larvae and spat of C. gigas. Detection of this pathogen is usually performed by PCR and/or in situ hybridization. This presentation will focus on the development of a new molecular diagnostic tool for OsHV-1 (and OsHV-1 variant 1) detection: the mini-array. This detection system, which allows multiple pathogens detection, combines viral DNA amplification by PCR and a specific hybridisation on a nylon membrane, revealed by a colorimetric reaction (DNA chip). Multiplex PCR involves...
Tipo: Text
Palavras-chave: Diagnostic; Crassotrea gigas; Oyster; Detection; Herpesviridae; OsHV 1.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3652.pdf

Project ROMEO : E. C. n° FA-S2 9052 - Research objectives : Mortality in European Oysters - Final Report
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Renault, Tristan; Arzul, Isabelle.
Crassostrea gigas is increasingly being cultured in a great number of countries from animaIs originally introduced from Japan, and is one of the most exploited bivalve species in the EU (FAO, 1989). Although no serious pathogen was detected previously in Japanese oysters in Europe, mortalities have been observed since the introduction of this bivalve species into the EU. Thus, oyster cultivation may be endangered by the occurrence of epizootics, especially virus diseases, which are considered one of the maj or risks to production. Indeed, mortalities have been described among different species of ostreids and are associated with the presence of viruses belonging to various families. The first description of a virus was reported in adult Eastern oyters,...
Tipo: Text
Palavras-chave: Pathology; Virology; Herpes-like infection; Detection; Diagnosis; Herpesviridae; Oyster; Crassostrea gigas; Mortality.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00044/15536/12915.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" Third Periodic Progress Report : 4th January 2001 to 3rd January 2002
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Herpesvirus infections have been reported in different bivalves species around the world. Specific diagnostic tools are needed in order ta assess the causative raIe of these agents in mortalities observed among bivalves There is currently a lack of information concerning the occurrence of bivalve herpesviruses. This is probably due ta the lack of suitable diagnostic tools. The basic method for examination of bivalve samples is predominantly histology. This enables the identification of any cellular changes, but is not conclusive for virus identification. Transmission electron microscopy is a necessity for visual confirmation of viral infections. However, histology and electron microscopy are time consuming and inadequate for epidemiological studies. The...
Tipo: Text
Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Virus; Oyster; Detection; OsHV-1; Herpesviridae.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00044/15524/12911.pdf

Rapid detection and quantification of the marine toxic algae, Alexandrium minutum, using a super-paramagnetic immunochromatographic strip test
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Gas, Fabienne; Baus, Beatrice; Quere, Julien; Chapelle, Annie; Dreanno, Catherine.
The dinoflagellates of Alexandrium genus are known to be producers of paralytic shellfish toxins that regularly impact the shellfish aquaculture industry and fisheries. Accurate detection of Alexandrium including A. minutum is crucial for environmental monitoring and sanitary issues. In this study, we firstly developed a quantitative lateral flow immunoassay (LFIA) using super-paramagnetic nanobeads for A. minutum whole cells. This dipstick assay relies on two distinct monoclonal antibodies used in a sandwich format and directed against surface antigens of this organism. No sample preparation is required. Either frozen or live cells can be detected and quantified. The specificity and sensitivity are assessed by using phytoplankton culture and field samples...
Tipo: Text
Palavras-chave: Alexandrium minutum; Detection; Quantification; Dipstick lateral flow immunoassay; Super-paramagnetic.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00286/39695/38152.pdf

Use of ROV's in operation of EAN underwater installation in the North Sea.
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Ricci, F.
The purpose of the paper is to present the state of the Art in use of ROV for assistance in offshore operations. This is done through a review of operations and gained experience during East Frigg Field diverless installation in 1988 and 10 years or under water Inspection, Maintenance and Repair (IMR) on all Fields operated by Elf Aquitaine Norge A/S (EAN). Basic requirements to the types of ROVs are given and the cost-efficiency of the operations are discussed. The potential for further development of the ROVs to replace divers for a large part of annual IMR tasks below water is addressed.
Tipo: Text
Palavras-chave: Steel structures; Detection; Cracks; Surveillance and enforcement; Pipelines; Offshore structures; Underwater inspection; Offshore operations; Marine technology; Offshore; Unmanned vehicles.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/1990/acte-1164.pdf

Diagnosis of oyster herpes-like virus: development and validation of molecular immunological and cellular tools "VINO". Second periodic progress report: 4th January 2000 at 3rd January 2001
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using teclmiques developed by the patiners to characterise viruses by studying their genome and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpesvirus (OsHV-1) DNA sequence with determination of the genome structure. 2 - Comparing OsHV -1 with viruses belonging to the Herpesviridae family on the basis of sequence data and genome structure. 3 -...
Tipo: Text
Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00048/15947/13377.pdf

Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan.
Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1...
Tipo: Text
Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf

Observing and Studying Extreme Low Pressure Events with Altimetry
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Carrere, Loren; Mertz, Francoise; Dorandeu, Joel; Quilfen, Yves; Patoux, Jerome.
The ability of altimetry to detect extreme low pressure events and the relationship between sea level pressure and sea level anomalies during extra-tropical depressions have been investigated. Specific altimeter treatments have been developed for tropical cyclones and applied to obtain a relevant along-track sea surface height (SSH) signal: the case of tropical cyclone Isabel is presented here. The S- and C-band measurements are used because they are less impacted by rain than the Ku-band, and new sea state bias (SSB) and wet troposphere corrections are proposed. More accurate strong altimeter wind speeds are computed thanks to the Young algorithm. Ocean signals not related to atmospheric pressure can be removed with accuracy, even within a Near Real Time...
Tipo: Text
Palavras-chave: Barotropic model; Sea level pressure; Extra tropical depressions; Tropical cyclones; Detection; Altimetry.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6350.pdf

Analytical Methods for Virus Detection in Water and Food
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bosch, Albert; Sanchez, Gloria; Abbaszadegan, Morteza; Carducci, Annalaura; Guix, Susana; Le Guyader, Soizick; Netshikweta, Rembuluwani; Pinto, Rosa M.; Van Der Poel, Wim H. M.; Rutjes, Saskia; Sano, Daisuke; Taylor, Maureen B.; Van Zyl, Walda B.; Rodriguez-lazaro, David; Kovac, Katarina; Sellwood, Jane.
Potential ways to address the issues that relate to the techniques for analyzing food and environmental samples for the presence of enteric viruses are discussed. It is not the authors' remit to produce or recommend standard or reference methods but to address specific issues in the analytical procedures. Foods of primary importance are bivalve molluscs, particularly, oysters, clams, and mussels; salad crops such as lettuce, green onions and other greens; and soft fruits such as raspberries and strawberries. All types of water, not only drinking water but also recreational water (fresh, marine, and swimming pool), river water (irrigation water), raw and treated sewage are potential vehicles for virus transmission. Well over 100 different enteric viruses...
Tipo: Text
Palavras-chave: Enteric viruses; Gastroenteritis; Hepatitis; Detection; Concentration.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00031/14191/11465.pdf

Vibrio nigripulchritudo monitoring and strain dynamics in shrimp pond sediments
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Walling, Emilie; Vourey, Elodie; Ansquer, Dominique; Beliaeff, Benoit; Goarant, C.
Aims: A description of bacterial pathogens in shrimp ponds is necessary to understand their pathogenesis. Vibrio nigripulchritudo was shown to contain saprophytic and pathogenic strains among New Caledonian isolates. We established a method to map the development of V. nigripulchritudo in pond sediments at three different genetic levels: the species level, then at the pathogenic cluster level and finally at the plasmid level, present only in all highly pathogenic isolates. Methods and Results: PCR methods were applied to shrimp pond sediments both before and after a mortality outbreak. Using crude samples, the species V. nigripulchritudo is not detected at first (0/42 samples at day 56 post stocking) but appears frequently in the sediments after the...
Tipo: Text
Palavras-chave: Detection; Diagnosis; Diseases; Ecology; PCR; Plasmids; Soil; Virulence.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00004/11484/8177.pdf

Detection de fissure sur structure en mer en presence de salissure marine projet "Crabe"
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Daste, S.
The "CRABE" method is a crack detection technique based on the use of low electromagnetic frequencies. This (patented) method relies on a global injection of current within the structure under inspection. The presence of a crack shall cause a disruption in the current flow, and consequently of the magnetic field off the surface in the vicinity of a defect. It is this magnetic signature which is detected by the magnetic sensor used with "CRABE". The major advantage of this process, developed by TRAVOCEAN and the LETI Division of CEA with the help of IFREMER, appears clearly: No preliminary cleaning of the welds to be inspected is required, since no contact is necessary. Similarly, it shall be possible to inspect the whole of structures to a total cost all...
Tipo: Text
Palavras-chave: Offshore structures; Fouling; Underwater exploration; Steel structures; Sensors; Cracks; Detection; Unmanned vehicles; Marine technology.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/1990/acte-1161.pdf

Foodborne viruses: Detection, risk assessment, and control options in food processing
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Bosch, Albert; Gkogka, Elissavet; Le Guyader, Soizick; Loisy-hamon, Fabienne; Lee, Alvin; Van Lieshout, Lilou; Marthi, Balkumar; Myrmel, Mette; Sansom, Annette; Schultz, Anna Charlotte; Winkler, Anett; Zuber, Sophie; Phister, Trevor.
In a recent report by risk assessment experts on the identification of food safety priorities using the Delphi technique, foodborne viruses were recognized among the top rated food safety priorities and have become a greater concern to the food industry over the past few years. Food safety experts agreed that control measures for viruses throughout the food chain are required. However, much still needs to be understood with regard to the effectiveness of these controls and how to properly validate their performance, whether it is personal hygiene of food handlers or the effects of processing of at risk foods or the interpretation and action required on positive virus test result. This manuscript provides a description of foodborne viruses and their...
Tipo: Text
Palavras-chave: Virus; Detection; Risk assessment; Food; Processing technologies.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00443/55507/57057.pdf

Detection of ostreid herpesvirus-1 (OsHV-1) by PCR using a rapid and simple method of DNA extraction from oyster larvae
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Batista, Frederico; Taris, Nicolas; Boudry, Pierre; Renault, Tristan.
A DNA extraction procedure was developed for the detection of ostreid herpesvirus-1 (OsHV-1) using the polymerase chain reaction (PCR) in oyster larvae. The DNA extraction procedure developed was tested on 8 larval samples. Abnormal nuclei with characteristic features associated with OsHV-1 infections were only observed in samples in which the viral DNA was detected by PCR. A previously described competitive PCR method was applied to detect inhibition during PCR reactions. The results show that the method can be used on small amounts of oyster larvae (3 mg) for the detection of OsHV-1 DNA by PCR.
Tipo: Text
Palavras-chave: DNA extraction; Detection; Larvae; Oyster; OsHV 1; Herpesvirus.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2916.pdf

Application du laser a la detection sous-marine: Experimentation d'un imageur
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Kervern, G; Legall, A.
Nowadays, side-looking sonar is the only equipment able to carry out long-range underwater imaging. The use of optics is limited to short-range identification by means of conventional video-cameras. However, active optical systems (lidars) manufacturing, using a blue-green laser, will enable optics to hold its place in the field of long-range underwater imaging. After a short comparison between acoustics and optics in underwater detection and a description of the proper features of underwater optical propagation, we present the different conceivable imaging principles and propose a method to determine their performances. The experimentations described concern a lidar imager mock-up using one of the imaging principles proposed. The pictures obtained deal...
Tipo: Text
Palavras-chave: Optics; Lasers; Detection; Acoustics; Underwater cameras; Sonar imagery; Imaging techniques; Underwater photography; Marine technology; Side scan sonar.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/1990/acte-1139.pdf

Diazonium Salt-Based Surface-Enhanced Raman Spectroscopy Nanosensor: Detection and Quantitation of Aromatic Hydrocarbons in Water Samples
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Tijunelyte, Inga; Betelu, Stephanie; Moreau, Jonathan; Ignatiadis, Ioannis; Berho, Catherine; Lidgi-guigui, Nathalie; Guenin, Erwann; David, Catalina; Vergnole, Sebastien; Rinnert, Emmanuel; Lamy De La Chapelle, Marc.
Here, we present a surface-enhanced Raman spectroscopy (SERS) nanosensor for environmental pollutants detection. This study was conducted on three polycyclic aromatic hydrocarbons (PAHs): benzo[a]pyrene (BaP), fluoranthene (FL), and naphthalene (NAP). SERS substrates were chemically functionalized using 4-dodecyl benzenediazonium-tetrafluoroborate and SERS analyses were conducted to detect the pollutants alone and in mixtures. Compounds were first measured in water-methanol (9:1 volume ratio) samples. Investigation on solutions containing concentrations ranging from 10−6 g L−1 to 10−3 g L−1 provided data to plot calibration curves and to determine the performance of the sensor. The calculated limit of detection (LOD) was 0.026 mg L−1 (10−7 mol L−1) for...
Tipo: Text
Palavras-chave: Polynuclear aromatic hydrocarbon (PAH); Surface-enhanced Raman spectroscopy (SERS); Nanosensor; Diazonium salt; Surface functionalization; Detection.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00386/49772/50316.pdf

Molecular detection and quantification of the protozoan Bonamia ostreae in the flat oyster, Ostrea edulis
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Robert, Maeva; Garcia, Celine; Chollet, Bruno; Lopez-flores, Inmaculada; Ferrand, Sylvie; Francois, Cyrille; Joly, Jean-pierre; Arzul, Isabelle.
Bonamia ostreae is an intracellular protozoan which is recognized as a cause of mortality in European populations of flat oysters (Ostrea edulis). Based on the recent characterization of actin genes of B. ostreae, specific primers were designed for real-time PCR using SYBR® Green chemistry. Specificity was demonstrated by the unique melting temperature peak observed in positive samples and by the lack of amplification in samples of oysters infected by closely related parasites, including Bonamia exitiosa. A calibration curve using a cloned template was defined to estimate copy number. The assay had a 6 log- dynamic range, mean inter- and intra-assay variation coefficients of &lt;1% and a minimum detection limit of 50 gene copies per reaction. Using...
Tipo: Text
Palavras-chave: Heart imprints; Ostrea edulis; Quantification; Detection; Real time PCR; Bonamia ostreae.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6934.pdf

Molecular identification of Vibrio tapetis, the causative agent of the brown ring disease of Ruditapes philippinarum
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Paillard, Christine; Gausson, Sabrina; Nicolas, Jean-louis; Le Pennec, Jean Paul; Haras, Dominique.
Vibrio tapetis is the marine bacterium responsible for the brown ring disease (BRD) affecting die manila clam, Ruditapes philippinarum. Identification of V tapelis has been previously performed using biochemical criteria and serological procedures. All of these methods are time consuming and ill-adapted to individual screening. This study describes an oligonucleotidic probe (Vt446) and two PCR primers, deduced from the 16S rDNA sequence, allowing a fast and specific V tapetis identification using dot blot hybridisation and species-specific primed PCR (SSP-PCR). The probe and primers have been tested on 60 strains, including referenced Vibrio sp., Gram negative and positive bacteria, marine bacteria samples and isolated clam bacteria. For all the 19 V...
Tipo: Text
Palavras-chave: DOT BLOT hybridisation; SSP PCR; Vibriosis; Bivalve; Detection; Diagnostic.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1692.pdf

A long term study of bonamiosis in Quiberon bay, France
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Berthe, Franck.
Bonamiosis was first reported in association with mass mortality of flat oysters, Ostrea edulis, in June 1979 in L'Ile Tudy, Brittany. The disease rapidly spread to all of the oyster farming areas in France but also in other European countries. The French flat oyster production, which already suffered from another protozoan disease, marteiliosis, decreased from 5500 mt in 1979 to less than 2000 mt after 1980. In 2001, about 350 French farms sell 1650 mt of flat oysters. This production mainly relies on natural spat collection which specially occurs in Quiberon bay. One third of this spat is transferred from South to North Brittany for further growth. Quiberon bay constitutes an interesting site regarding the surveillance of bonamiosis because of the...
Tipo: Text
Palavras-chave: Detection; Bonamia ostreae; Protozoan; Diseases; Pathology; Ostrea edulis; Oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3314.pdf

An intercomparison study of the determination of sulfite in tropical shrimps by the West European Fish Technologists' Association (WEFTA)
Provedor de dados: Open Marine Archive
Autores: Luten, J.; Bouquet, W.; Oehlenschläger, J.; Meetschen, U.; Etienne, M.; Stroud, G.; Bykowski, P.; Batista, I.; Vyncke, W.; Stefansson, G.
Eight WEFTA laboratories participated in two series of intercomparison exercises on sulfite determination in tropical shrimps. Samples of tropical shrimps were spiked with sodium metabisulfite (Na&lt;sub&gt;2&lt;/sub&gt;S&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;5&lt;/sub&gt;) and hydroxymethylsulfonate (HMS) at a level of 25-90 mg SO&lt;sub&gt;2&lt;/sub&gt;/kg. Most of the laboratories determined the sulfite content with (modified) methods of Monier-Williams or De Vries et al. The overall mean recovery of sulfite was rather low (47-60%), which may be attributed to an irreversible reaction of sulfite with the tropical shrimps. The repeatability of the methods within the participating laboratories was good. However, reproducibility among the laboratories was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detection; Fishery products; Food technology; International cooperation; Quality control; Recovery; Shrimp fisheries; Sulphites.
Ano: 1997
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=5742

Detection by Rt-Pcr and Molecular Characterization of Tremovirus A Obtained from Clinical Cases of Avian Encephalomyelitis (AE) Outbreaks in Brazil
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Torre,D De la; Nuñez,LF; Parra,SHS; Astolfi-Ferreira,CS; Ferreira,AJP.
ABSTRACT This study determined the presence of Tremovirus A as the possible agent related to Avian Encephalomyelitis in broiler chicks from the states of São Paulo (SP) Paraná (PR), Goiás (GO), Santa Catarina (SC) and Rio Grande do Sul (RS), between the years 2006 and 2015. Samples of the nervous, digestive, respiratory, immune, and renal systems, plus muscular organs from broiler chicks with neurological problems such as ataxia and muscle tremors, and four (4) commercial vaccines as positive control, were tested by reverse-transcriptase (RT-PCR) amplification and DNA sequencing. A highly conserved region (P1) of the viral genome, was used to amplify a segment which encodes a structural protein VP4. Out of 112 samples, 46 were positive (42%) for Tremovirus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Tremovirus A; Avian Encephalomyelitis; Detection; Molecular characterization.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300527

Occurrence of infectious Laryngotracheitis Virus (ILTV) in 2009-2013 in the State of São Paulo - Brazil
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Parra,SHS; Nuñez,LFN; Astolfi-Ferreira,CS; Ferreira,JP.
Infectious laryngotracheitis is a very important respiratory disease because it causes significant economic losses in the poultry industry. The target of ILTV infections is the respiratory system, and the main organ in which the virus remains latent is the trigeminal ganglia. However, the virus has demonstrated tropism for other organs as well. The present study was conducted to determine the presence of Infectious Laryngotracheitis Virus (ILTV) in the state of São Paulo. Samples submitted to LABOR- USP during the last four years (2009-2013) analyzed by a nested/PCR technique. Out of the 682 samples from layers tested for LTIV, 12.46 % were positive, and derived from in both traditional (trachea and trigeminal ganglion) and untraditional (cecal tonsils,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious Laryngotracheitis; PCR; Detection; Trachea; Trigeminal.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000100117

Evaluation of the Direct and Enrichment Iso 10272 Methods for the Detection of Campylobacter Spp. in Chicken Meat
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Benetti,TM; Abrahão,WM; Ferro,ID; Macedo,REF; Oliveira,TCRM.
ABSTRACT In 2006, the International Organization for Standardization (ISO) published two standard protocols for the detection and enumeration of Campylobacter spp. in foods: the ISO 10272-2(direct method) and the ISO 10272-1 (enrichment method). The aim of this study was to compare the efficiency of these two methods in the detection of Campylobacter spp. and propose a modification in the enrichment method. Compared with the enrichment method, the direct method yielded a higher number of positive results for Campylobacter spp. and, consequently, presented higher sensitivity percentage. The isolation of Campylobacter spp. was more difficult when 10 mL of rinse was used in the enrichment method, which is currently recommended by the ISO protocol. Therefore,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter spp.; Detection; ISO 10272; Refrigerated chicken carcass.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100023

Infectious bronchitis virus: detection and vaccine Strain differentiation by semi-nested RT-PCR
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Okino,CH; Montassier,MFSM; Givisiez,PEN; Furuyama,CRAG; Brentano,L; Montassier,HJ.
A semi-nested reverse transcription-polymerase chain reaction (Semi-N-RT-PCR) was developed and used to detect the S glycoprotein gene of infectious bronchitis virus (IBV) strains and to discriminate H120 vaccine strain from other strains. Viral RNA was extracted from the allantoic fluid of chicken embryos and from tissues of chickens experimentally infected with different strains of IBV. Amplification and identification of the viral RNA was performed using two sets of primers complementary to a region of the S glycoprotein gene in the Semi-N-RT-PCR assay. The pair of primers used in the first PCR consisted of universal oligonucleotides flanking a more variable region of S1-S2 gene. The second primer pair was used in the Semi-N-RT-PCR and was comprised of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detection; Differentiation; Infectious bronchitis virus; Semi-nested RT-PCR; Vaccine strain.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000100010

First report of Pestalotiopsis diospyri causing canker on persimmon trees
Provedor de dados: Revista Brasileira de Fruticultura
Autores: Alves,Giselda; Verbiski,Francineia Silva; Michaelides,Themis J.; May-de Mio,Louise Larissa.
During 2006 to 2009 season symptoms of a canker disease were observed on twigs and branches of young and mature persimmon trees (Diospyros kaki L.) cv. Fuyu in the States of Santa Catarina and Paraná in the Southern Brazil. The cankers result in severe damage and reduced production. Isolations from the margins of these cankers revealed a genus of Pestalotiopsis. Koch's postulates were confirmed using two isolates of the pathogen which was identified as Pestalotiopsis diospyri.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disease; Inoculation; Detection; Diospyros kaki.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000300039

Low cost production and purification of polyclonal antibodies to staphylococcal enterotoxin A
Provedor de dados: Revista de Microbiologia
Autores: Oliveira,Tereza Cristina R.M. de; Hirooka,Elisa Yoko.
An immunization scheme for production of antiserum to staphylococcal enterotoxin A (SEA) is proposed. The reference method of Robbins and Bergdoll was modified to reduce the number of doses and the amount of toxin used per animal. The best immunization scheme used injections in days 0, 8, 24, 59, 62 and 67. The amount of toxin at each injection was 5, 6, 20, 50, 100 and 200&lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;g, respectively. The total amount of toxin was 381&lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;g, which corresponded to a reduction of 107&lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;g in the amount of toxin for each animal when compared to the reference method. The average antiserum titer using the Optimum Sensitivity Plate - OSP was 1:60 and using ELISA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Enterotoxins; Detection; Immunization.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200006

Molecular detection of Potato yellow vein virus in the natural whitefly vector Trialeurodes vaporariorum, Westwood
Provedor de dados: Revista de Protección Vegetal
Autores: Barragan,Carlos Eduardo; Guzmán-Barney,Mónica.
Potato yellow vein virus (PYVV) expresses yellowing symptoms on plants and it is transmitted by the whitefly vector Trialeurodes vaporariorum Westwood. No molecular viral detection from the vector extracts has been reported to date. The objectives of this study were I) amplification of the PYVV major coat protein (CP) gene from viruliferous vector extracts by RT-PCR using specific primers, II) cloning and III) sequencing and sequences analysis. Some Colombian Solanum phureja (Jus et Buk) field potato plants expressing yellowing symptoms and confirmed PYVV positive by RT-PCR were used as virus donor plants for feeding whiteflies. Viruliferous vectors were obtained by depositing aviruliferous whiteflies on symptomatic leaves and kept in a muslin...
Tipo: Journal article
Palavras-chave: PYVV; RNA; Whitefly; Detection; Vector.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000300002

Identification of geneticaly modified soybean seeds resistant to glyphosate
Provedor de dados: Scientia Agricola
Autores: Tillmann,Maria Ângela André; West,Shirlie.
Advances in genetic engineering permit the modification of plants to be tolerant to certain herbicides that are usually not selective. For practical and commercial purposes, it is important to be able to detect the presence or absence of these traits in genotypes. The objective of this research was to develop a procedure for identifying genetically modified soybean (Glycine max L. Merr.) with resistance to the herbicide glyphosate. Two studies were conducted based on germination test. In the first study, soybean seeds were pre-imbibed in paper towel with the herbicide solutions, then transferred to moist paper towel for the germination test. In the second study, seeds were placed directly in herbicide solutions in plastic cups and tested for germination...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenic; Germination; Detection; Glyphosate.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000300017

วิธีทางซีรัมวิทยาในการตรวจหา Potato Virus X (PVX) จากพืชที่ได้จากการเพาะเลี้ยงเนื้อเยื่อและหัวพันธุ์มันฝรั่ง
Provedor de dados: Thai Agricultural Research Repository
Autores: Sumerng Kumthong.
Thesis (M.Sc. in Agriculture)
Tipo: PhysicalObject
Palavras-chave: Potato; Solanum tuberosum; Plant; Seed; Potato Virus X; PVX; Serological method; Detection; Tissue culture; มันฝรั่ง; พืช; หัวพันธุ์มันฝรั่ง; ท่อนพันธุ์; ไวรัส; วิธีทางซีรัมวิทยา; การตรวจเชื้อ; การเพาะเลี้ยงเนื้อเยื่อ.
Ano: 1987
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5474

Garlic viral complex: identification of Potyviruses and Carlavirus in Central Brazil
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: FAJARDO,THOR V. M.; NISHIJIMA,MARTA; BUSO,JOSÉ A.; TORRES,ANTÔNIO C.; ÁVILA,ANTÔNIO C.; RESENDE,RENATO O.
Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: OYDV-G; LYSV; GCLV; Detection; Coat protein; PCR; Probes; Epidemiology.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300007

Diurnal and seasonal variability in bird counts in a forest fragment in southeastern Brazil
Provedor de dados: Zoologia
Autores: Antunes,Alexsander Z.
Diurnal variation in detection can influence bird census results. I measured variation in daily and seasonal detections of birds in a forest fragment at Barreiro Rico ranch, Anhembi, state of São Paulo. Birds were recorded on transects of unlimited distance, sampled one day per month, dawn to dusk, between December 2000 and March 2001 (rainy season) and between May and August 2001 (dry season). Significant daily variation in detection occurred during the rainy season, with most detections between dawn and 9 h. In the dry season detections declined for total numbers of individuals and species and most detections occurred during late morning and mid afternoon. Detection patterns might reflect seasonal variation in bird behavior rather than seasonal variation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atlantic Forest; Census; Detection; Seasonal variation; Transects.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752008000200011

Employment of the 18s rRNA screening PCR technique in the detection of Equine Piroplasmosis, in horses of sports and military operations, of the Brazilian Army
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Sousa,C.P.; Soares,J.G.B.
ABSTRACT The present work had the objective of detecting the occurrence of Equine Piroplasmosis in horses housed in the 3rd Guards Cavalry Regiment (GCR) - Brazilian Army (BA) ˗ Porto Alegre, RS-Brazil, as well as to demonstrate the proactivity of PCR (Polymerase Chain Reaction) technique, aiming at the judicious use of the resources involved in the training and employment of Equines in the Brazilian Army. Fifty horses of the 3rd GCR - Porto Alegre ˗ RS, which are employed for Sport, Military Ceremonial, Law and Order Guarantee Operations (LOGO), were evaluated by means of the 18s r RNA screening with PCR technique, thirty eight horses with Babesia Caballi and Theileria Equi were detected, which corresponds to an incidence of 76% of the horses effective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horses; Babesia Caballi; Detection; PCR; LOGO; GCR; BA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601680

First molecular and serological detection of Epizootic Hemorrhagic Disease virus in white tailed deer ( Odocoileus virginianus ) from Tamaulipas, Mexico
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Merino,J.O.; Cruz,NI. De la; Galvan,G.; León,A.P. De; Burnes,J.
ABSTRACT Epizootic hemorrhagic disease viruses (EHDV) are dsRNA arboviruses transmitted by biting midges of the genus Culicoides that cause disease in domestic and wild ruminants. Epizootic hemorrhagic disease (EHD) is considered the most important infectious disease of white tailed deer (WTD) in North America, some studies in Northeast Mexico reported EHDV-seropositive WTD and EHDV-infected Culicoides vectors. The increasing population of WTD that share habitat with livestock in Northeast México highlights the importance of EHD for the livestock industry in the transboundary region with the U.S. One hundred and twenty two samples from WTD in Tamaulipas state, Mexico were tested by ELISA and RT-PCR for EHDV antibodies and nucleic acid, respectively. Twelve...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Deer; Detection; EHDV; RT-PCR; Serology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100077

Identification and characterization of Fusarium mangiferae as pathogen of mango malformation in India
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Kumar,Pradeep; Kamle,Madhu; Misra,Asok Kumar; O'Donovan,Anthonia; Pagano,Marcela; Modi,Dinesh Raj.
ABSTRACT Fusarium mangiferae (=F. subglutinans) isolates collect from malformed samples from major mango-growing area of North India. Molecular identification and characterization of eleven most virulent isolates of F. mangiferae, based on pathogenicity tests used for the present study. Species-specific, genus specific ITS-PCR and PCR-RFLP performed for the accurate and easy detection of F. mangiferae. The rDNA-ITS 28S region sequences used for phylogenetic analysis of Fusarium isolates from India and other countries for homology search between them. The phylogenetic tree divided the isolates into three clades (i.e., American, Asian and African) and showed the high level of sequence based similarity (69-99%) among all Fusarium sequences from Asia. Thus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusarium mangiferae; Detection; Phylogenetic analysis; Vegetative malformation; Floral malformation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100204

Use of cardiopulmonary exercise testing to assess early ventilatory changes related to occupational particulate matter
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Chao,T.P.; Sperandio,E.F.; Ostolin,T.L.V.P.; Almeida,V.R.; Romiti,M.; Gagliardi,A.R.T.; Arantes,R.L.; Dourado,V.Z.
Spirometry has been used as the main strategy for assessing ventilatory changes related to occupational exposure to particulate matter (OEPM). However, in some cases, as one of its limitations, it may not be sensitive enough to show abnormalities before extensive damage, as seen in restrictive lung diseases. Therefore, we hypothesized that cardiopulmonary exercise testing (CPET) may be better than spirometry to detect early ventilatory impairment caused by OEPM. We selected 135 male workers with at least one year of exposure. After collection of self-reported socioeconomic status, educational level, and cardiovascular risk data, participants underwent spirometry, CPET, body composition assessment (bioelectrical impedance), and triaxial accelerometry (for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Occupational exposure to particulate matter; Cardiopulmonary exercise testing; Detection; Spirometry; Ventilatory impairment.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500612

Kidney health for everyone everywhere – from prevention to detection and equitable access to care
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Li,P. Kam-Tao; Garcia-Garcia,G.; Lui,Siu-Fai; Andreoli,S.; Fung,W. Wing-Shing; Hradsky,A.; Kumaraswami,L.; Liakopoulos,V.; Rakhimova,Z.; Saadi,G.; Strani,L.; Ulasi,I.; Kalantar-Zadeh,K.
The global burden of chronic kidney disease (CKD) is rapidly increasing with a projection of becoming the 5th most common cause of years of life lost globally by 2040. CKD is a major cause of catastrophic health expenditure. The costs of dialysis and transplantation consume up to 3% of the annual healthcare budget in high-income countries. However, the onset and progression of CKD is often preventable. In 2020, the World Kidney Day campaign highlights the importance of preventive interventions – be it primary, secondary, or tertiary. This article focuses on outlining and analyzing measures that can be implemented in every country to promote and advance CKD prevention. Primary prevention of kidney disease should focus on the modification of risk factors and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Kidney diseases; Prevention; Detection; Awareness.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300101

Zika detection: comparison of methodologies
Provedor de dados: Brazilian Journal of Microbiology
Autores: Colombo,Tatiana Elias; Terzian,Ana Carolina Bernardes; Araújo Júnior,João Pessoa; Parreira,Ricardo; Cabrera,Eliana Márcia Sotello; Santos,Izalco Nuremberg Penha dos; Reis,Andréia Francesli Negri; Costa,Fabiana Rodrigues; Cruz,Lilian Elisa Arão Antônio; Rombola,Patrícia Lopes; Nogueira,Maurício Lacerda.
ABSTRACT Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan® RT-qPCR assay. We used a SYBR® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan® RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR® Green RT-qPCR based on Tm comparison;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zika virus; Detection; Sensitivity; Specificity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100144

Rapid identification of Mycobacterium tuberculosis complex by a novel hybridization signal amplification method based on self-assembly of DNA-streptavidin nanoparticles
Provedor de dados: Brazilian Journal of Microbiology
Autores: Wang,Haihe; Zhao,Chunyan; Li,Fan.
Rapid detection of Mycobacterium tuberculosis complex (MTBC) is a critical step in controlling tuberculosis (TB). In this study, we used IS6110 as the specific identification target to develop a novel hybridization signal amplification method (HSAM) for the rapid and direct detection of MTBC from clinical sputum specimens. This system consists of magnetic bead-linked capture probes for target isolation, dextranbased nanoparticles for amplifying the reporter molecule (biotinylated-FITC), and detection probes (2B-DNA) for binding the nanoparticles. Both the capture and detection probes were specific to the IS6110 target sequence. Our results determined that as few as 10 copies of the IS6110 sequence or 10 M. tuberculosis bacteria could be detected,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis complex (MTBC); Detection; Diagnosis; Rapid methods; Identification.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300016

16s-23S rDNA: polymorphisms and their use for detection and identification of Xylella fastidiosa strains
Provedor de dados: Brazilian Journal of Microbiology
Autores: Martinati,Juliana Camargo; Pacheco,Flávia Tereza Hansen; Miranda,Vitor Fernandes Oliveira de; Tsai,Siu Mui.
Strains of Xylella fastidiosa from several hosts (coffee, citrus, grape, almond, oleander, peach, plum, etc.) were characterized by analyzing the content of the nucleotide sequences of 16S-23S rDNA (coding for a small subunit ribosomal RNA) spacer region (ITS). Current methods for sequencing the ITS region yields partial sequences that do not contribute with significant information. According to this fact, new primers have been designed in order to obtain a complete sequence and facilitate the sequencing. The complete 16S-23S sequences from 08 strains were amplified through PCR using the primers designed in our lab. The 16S-23S sequences obtained were compared with 52 others sequences entries in GenBank database. The results revealed a higher level of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; 16S-23S rDNA; Intergenic spacer; Identification; Detection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100033

Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene
Provedor de dados: Brazilian Journal of Microbiology
Autores: Wang,Jiashun; Li,Yi; Chen,Jia; Hua,Deping; Li,Yi; Deng,Hui; Li,Ying; Liang,Zhixuan; Huang,Jinhai.
Abstract Detection of Salmonella is very important to minimize the food safety risk. In this study, the recombinant PagC protein and PagC antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture Salmonella cells from pork and milk samples. And then the SYBR Green qualitative PCR was developed to detect the pathogenic Salmonella. The results showed that the PagC polyclonal antiserum is of good specificity and the capture rate of 0.1 mg IMBs for Salmonella tended to be stable at the range of 70-74% corresponding to the concentrations between 101 and 104 CFU/mL. The method developed demonstrated high specificity for the positive Salmonella samples when compared to non-specific DNA samples, such as Escherichia coli, Staphylococcus aureus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; IMBs-qPCR; PagC gene; Detection.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200320

Quick adaptation of Ralstonia Solanacearum to copper stress to recover culturability and growth in water and soil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Ascarrunz,Sergio Daniel Moreira; Natsuaki,Tomohide; Honjo,Hitoshi; Fukui,Ryo.
Cells of Ralstonia solanacearum were exposed to Cu in distilled water, and the resulting Cu-stressed non-culturable cells were inoculated to natural (non-pasteurized) and pasteurized soils in order to examine their culturability and recovery. Exposing the cells to 20 µM CuSO4 produced transitory non-culturable cells, which exhibited a remarkable recovery in culturability after incubation in the solution for 36 h, reaching a density near the initial level by 108 h. To determine whether such non-culturable cells actually "resuscitated" or multiplied after adapting to Cu toxicity, growth curves were constructed in order to contrast the rates of increase in culturable cell numbers between Cu-stressed or non-stressed inocula. Additionally, fresh non-stressed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ralstonia solanacearum; Non-culturable cells; Adaptation to copper; Survival; Detection.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200023

Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment
Provedor de dados: Brazilian Journal of Microbiology
Autores: Oliveira,I.C.; Almeida,R.C.C.; Hofer,E.; Almeida,P.F.
A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 10(8) pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The protocol was faster...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria; Bacteriophage A511; Laser light; Detection; Skimmed milk.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300040

Rhinovirus detection using different PCR-based strategies
Provedor de dados: Brazilian Journal of Microbiology
Autores: Silva,E.R.M.; Watanabe,A.S.A.; Carraro,E.; Perosa,A.H.S.; Granato,C.F.H.; Bellei,N.C.J.
Human rhinoviruses (HRVs) are the major cause of the common cold. HRVs were recently reclassified into the Enterovirus genus (HEV) in the Picornaviridae family. HRVs and other members of the HEV genus share many common features, including sense RNA genomes and partial nucleotide sequence identity. The aim of this study was to evaluate different HRV detection strategies. Samples from adults with acute respiratory infection (n = 291) who were treated in Sao Paulo Hospital (2001-2003) were tested using three assays. The first assay detected picornaviruses by RT-PCR and hybridization, the second detected rhinoviruses using RT-PCR/sequencing, and the third differentiated HRV from HEV using duplex semi-nested-RT-PCR. Analysis of the results obtained from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhinovirus; Enterovirus; Detection; Differentiation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200038

Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Souza,Carine Kunzler; Streck,André Felipe; Gonçalves,Karla Ratje; Pinto,Luciane Dubina; Ravazzolo,Ana Paula; Barcellos,David Emílio dos Santos Neves de; Canal,Cláudio Wageck.
Abstract Ungulate tetraparvovirus 2 (UTV2) , formerly known as porcine hokovirus due to its discovery in Hong Kong, is closely related to a Primate tetraparvovirus (human PARV-4) and Ungulate tetraparvovirus 1 (bovine hokovirus). Until now, UTV2 was detected in European, Asian and North American countries, but its occurrence in Latin America is still unknown. This study describes the first report of UTV2 in Brazil, as well as its phylogenetic characterization. Tissue samples (lymph node, lung, liver, spleen and kidney) of 240 piglets from eight different herds (30 animals each herd) were processed for DNA extraction. UTV2 DNA was detected by PCR and the entire VP1/VP2 gene was sequenced for phylogenetic analysis. All pigs from this study displayed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Porcine; Tetraparvovirus; Phylogeny; Hokovirus; Detection; Ungulate.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200513

Gene detection and toxin production evaluation of hemolysin BL of Bacillus cereus isolated from milk and dairy products marketed in Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Reis,Andre L.S.; Montanhini,Maike T.M.; Bittencourt,Juliana V.M.; Destro,Maria T.; Bersot,Luciano S.
Bacillus cereusis an ubiquitous, spore-forming bacteria that can survive pasteurization and the majority of the heating processes used in the dairy industry. Besides, it is a pathogen responsible for different types of food poisoning. One type of foodborne disease caused by B.cereusis the diarrheal syndrome, which is caused by the ingestion of vegetative cells producing toxins in the small intestine. One virulence factor for the diarrheal syndrome is the toxin hemolysin BL (HBL), a three-component protein formed by the L1, L2 and B components. In order to evaluate the presence of diarrheal strains isolated from milk and dairy products, 63 B. cereus isolates were obtained from 260 samples of UHT milk, pasteurized milk and powdered milk, sold in commercial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Milk; Dairy products; Bacillus cereus; Pathogenicity; Detection.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400024

Detection of Salmonella sp. from porcine origin: a comparison between a PCR method and standard microbiological techniques
Provedor de dados: Brazilian Journal of Microbiology
Autores: Castagna,Sandra Maria Ferraz; Muller,Monika; Macagnan,Marisa; Rodenbusch,Carla Rosane; Canal,Cláudio Wageck; Cardoso,Marisa.
The aim of this study was to compare a polymerase chain reaction (PCR) method combined with selective enrichment in Rappaport-Vassiliadis broth (PCR-RVB) with standard microbiological techniques (SMT) for the generic detection of Salmonella in samples of porcine origin. Two hundred sixty eight field samples consisting of 42 sets of pooled porcine mandibular lymph nodes and tonsils, 44 samples of intestinal content, 38 pork sausage meat samples and 144 samples of feed collected from swine farms were submitted to the PCR-RVB and SMT protocols. Salmonella was detected in 54 samples using the PCR-RVB assay and in 42 samples by SMT, three of the SMT Salmonella-positive samples (one each of S. Derby, S. Panama and S. Typhimurium) being Salmonella-negative by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detection; PCR; Pork; Salmonella; Swine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400013

Detection of Salmonella spp, Salmonella Enteritidis and Typhimurium in naturally infected broiler chickens by a multiplex PCR-based assay
Provedor de dados: Brazilian Journal of Microbiology
Autores: Paião,F.G.; Arisitides,L.G.A.; Murate,L.S.; Vilas-Bôas,G.T.; Vilas-Boas,L.A.; Shimokomaki,M.
The presence of Salmonella in the intestinal tract, on the chickens skin and among their feathers, may cause carcasses contamination during slaughtering and processing and possibly it is responsible by the introduction of this microorganism in the slaughterhouses. A rapid method to identify and monitor Salmonella and their sorovars in farm is becoming necessary. A pre-enriched multiplex polymerase chain reaction (m-PCR) assay employing specific primers was developed and used to detect Salmonella at the genus level and to identify the Salmonella enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterica serovar Typhimurium (S. Typhimurium) in broiler chicken swab samples. The method was validated by testing DNA extract from 90 fresh culture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aviculture; Bacteria; Detection.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100005

Capture immunoassay for LT detection produced by enterotoxigenic Escherichia coli in bacterial isolates
Provedor de dados: Brazilian Journal of Microbiology
Autores: Menezes,Caroline Anunciação; Gonçalves,Danielle Silva; Amianti,Jackeline; Fernandes,Irene; Taddei,Carla Romano; Koga,Paula Célia Mariko; Trabulsi,Luiz Rachid; Martinez,Marina Baquerizo; Piazza,Roxane Maria Fontes.
A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassayis a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detection; Polyclonal antibody; Monoclonal antibody; Thermo-labile toxin; E. coli.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500004