Consulta: subjectFacets:"Karyotype"
Registros recuperados: 94
Data/hora: 09/06/2024 18:03:15

QUANTITATIVE KARYOTYPE OF DIPLODON CHILENSIS (GRAY 1828) (BIVALVIA:HYRIIDAE)
Provedor de dados: Gayana
Autores: Jara-Seguel,Pedro; Peredo,Santiago; Palma-Rojas,Claudio; Parada,Esperanza; Lara,Gladys.
The karyotype of a freshwater population of Diplodon chilensis located in La Poza area of the Villarrica Lake (39º18'S; 72º05'W), Southern Chile, was studied. The chromosomes were obtained by squash of cleaving embryos, previously treated with colchicine and fixed in ethanol-acetic acid 3:1 at 4ºC and stained using the Feulgen reaction. Diplodon chilensis showed a diploid karyotype of 2n = 34, with metacentric and submetacentric chromosomes. This work is the first approach to cytogenetic characterization of Chilean populations of this species.
Tipo: Journal article
Palavras-chave: Hyriidae; Diplodon chilensis; Karyotype; Freshwater; Bivalves; Southern Chile; Hyriidae; Diplodon chilensis; Cariotipo; Bivalvos dulceacuícolas; Sur de Chile.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200008

Karyological study of Amphisbaena ridleyi (Squamata, Amphisbaenidae), an endemic species of the Archipelago of Fernando de Noronha, Pernambuco, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Laguna,Marcia Maria; Amaro,Renata Cecília; Mott,Tamí; Yonenaga-Yassuda,Yatiyo; Rodrigues,Miguel Trefaut.
The karyotype of Amphisbaena ridleyi, an endemic species of the archipelago of Fernando de Noronha, in State of Pernambuco, Brazil, is described after conventional staining, Ag-NOR impregnation and fluorescence in situ hybridization (FISH) with a telomeric probe. The diploid number is 46, with nine pairs of macrochromosomes (three metacentrics, four subtelocentrics and two acrocentrics) and 14 pairs of microchromosomes. The Ag-NOR is located in the telomeric region of the long arm of metacentric chromosome 2 and FISH revealed signals only in the telomeric region of all chromosomes. Further cytogenetic data on other amphisbaenians as well as a robust phylogenetic hypothesis of this clade is needed in order to understand the evolutionary changes on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphisbaena ridleyi; Karyotype; Fernando de Noronha; Ag-NOR; FISH with telomeric probes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100011

Karyotypic description of four species of Trigona (Jurine, 1807) (Hymenoptera, Apidae, Meliponini) from the State of Mato Grosso, Brazil
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Kleber França; Brito,Rute Magalhães; Miyazawa,Carlos Suetoshi.
The genus Trigona contains at least 31 species, but there have been few cytogenetic studies of this group. In this work, four species of Trigona (T. branneri, T. chanchamayoensis, T. hyalinata, and T. recursa) from the municipality of Cuiabá, in the State of Mato Grosso, Brazil, were studied. In all of the species, the females had 2n = 34 chromosomes and the males had n = 17. The C-banding patterns showed that the karyotypes of these species consisted mainly of acrocentric and pseudoacrocentric chromosomes. These cytogenetic findings should useful in future phylogenetic studies of this group.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Karyotype; Meliponini; Stingless bees; Trigona.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200010

Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products
Provedor de dados: Genetics and Molecular Biology
Autores: Schneider,Marielle Cristina; Carraro,Bianca Pierina; Cella,Doralice Maria; Matiello,Rodrigo Rodrigues; Artoni,Roberto Ferreira; Almeida,Mara Cristina.
In this work we describe the cytogenetic analyses performed in specimens of Astylus variegatus (Germar, 1824) collected in two localities: one area of natural vegetation and one of agricultural crops, where agrochemical products were used. Astylus variegatus had karyotypes with 2n(male) = 16+Xy p and 2n (female) = 16+XXp, with exclusively metacentric chromosomes. Pachytene spermatocytes showed synapsed autosomal bivalents and non-associated sex chromosomes. In diplotene, the autosomal bivalents exhibited one or two terminal chiasmata and the Xy p had a typical parachute configuration. In meiotic cells of some specimens, an extra chromosome, interpreted as a B chromosome, was observed. C-banding showed constitutive heterochromatin in the pericentromeric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B chromosome; Constitutive heterochromatin; Karyotype; Meiosis; Ag-NORs.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400023

Chromosomal diversification of diploid number, heterochromatin and rDNAs in two species of Phanaeus beetles (Scarabaeidae, Scarabaeinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Arcanjo,Amanda; Cabral-de-Mello,Diogo Cavalcanti; Martins,Cesar; Moura,Rita de Cássia de; Souza,Maria José de.
The genus Phanaeus is included in the tribe Phanaeini, one of the most diverse tribes within the subfamily Scarabaeinae in terms of chromosomal characteristics. However, so far the species of this genus were not studied with differential cytogenetic techniques, limiting any inference of the probable mechanisms responsible for this diversity. In this work, several techniques were applied with the aim of cytogenetically characterizing two Phanaeus species. The karyotype found for Phanaeus (Notiophanaeus) chalcomelas was 2n = 12, neo-XY, and that of P. (N.) splendidulus was 2n = 20, Xy p, considered primitive for the family Scarabaeidae. The chromosomes of both species showed a high amount of constitutive heterochromatin (CH), with blocks rich in base pairs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Chromosome evolution; Repetitive DNA; FISH.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300007

Cytogenetic study of the giant otter Pteronura brasiliensis Zimmermann 1780 (Carnivora, Mustelidae, Lutrinae)
Provedor de dados: Genetics and Molecular Biology
Autores: Franco-de-Sá,Jorge Felipe Oliveira; Rosas,Fernando César Weber; Feldberg,Eliana.
The giant otter, Pteronura brasiliensis Zimmermann 1780 (Carnivora, Mustelidae, Lutrinae), was widely distributed in South America but stable populations are now only found in the Pantanal and Amazon regions and the species is classified as endangered. There is only one recognized species of giant otter, although two subspecies of doubtful value have also been cited in the literature. We present the first karyotype of four captive P. brasiliensis specimens, all of which posses 2n = 38 chromosomes as 14M+8SM+6ST+8A and one pair of sexual chromosomes. An heteromorphic secondary constriction, associated with the nucleolar organizer region (NOR), was seen on the long arms of chromosome pair 17. The C-banding technique revealed heterochromatin in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon; Aquatic mammals; Ariranha; Karyotype.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600011

Combined association of Presenilin-1 and Apolipoprotein E polymorphisms with maternal meiosis II error in Down syndrome births
Provedor de dados: Genetics and Molecular Biology
Autores: Bhaumik,Pranami; Ghosh,Priyanka; Ghosh,Sujay; Feingold,Eleanor; Ozbek,Umut; Sarkar,Biswanath; Dey,Subrata Kumar.
Abstract Alzheimer's disease and Down syndrome often exhibit close association and predictively share common genetic risk-factors. Presenilin-1 (PSEN-1) and Apolipoprotein E (APOE) genes are associated with early and late onset of Alzheimer's disease, respectively. Presenilin −1 is involved in faithful chromosomal segregation. A higher frequency of the APOE ε4 allele has been reported among young mothers giving birth to Down syndrome children. In this study, 170 Down syndrome patients, grouped according to maternal meiotic stage of nondisjunction and maternal age at conception, and their parents were genotyped for PSEN-1 intron-8 and APOE polymorphisms. The control group consisted of 186 mothers of karyotypically normal children. The frequencies of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome; Genetic polymorphism; Karyotype; Meiosis; Microsatellite markers.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400577

Cytogenetic analysis of five species of the subfamily Corydoradinae (Teleostei: Siluriformes: Callichthyidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Shimabukuro-Dias,Cristiane Kioko; Oliveira,Claudio; Foresti,Fausto.
In the present study, five callichthyid species belonging to the subfamily Corydoradinae were karyotyped: three species of Aspidoras and two of Corydoras. The three species of Aspidoras had the same diploid number, 2n = 46 chromosomes, similar karyotypic formulae, with most chromosomes metacentric or submetacentric, single interstitial Ag-NORs and C-band positive segments mainly found in the centromeric position. The comparative analysis of cytogenetic data available for the genus Aspidoras and other species of Corydoradinae suggest that several events of centric fusion occurred in the origin of the species of Aspidoras. The two analyzed species of Corydoras showed high diploid numbers, 2n = 74 in C. sodalis and 2n = 90 in C. britskii. While C. sodalis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish cytogenetic; Karyotype; NOR; C-banding; Karyotypic evolution.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400014

Cytogenetic data of Partamona peckolti (Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/CMA3 and DA/DAPI
Provedor de dados: Genetics and Molecular Biology
Autores: Brito,Rute Magalhães; Caixeiro,Ana Paula de Almeida; Pompolo,Silvia das Graças; Azevedo,Giselle Garcia.
The stingless bees of the Partamona genus have been studied taxonomically, ecologically and behaviourally, but cytogenetic studies are still rare. The objective of this study was to obtain cytogenetic data to contribute to Partamona peckolti species characterization. Heterochromatin was localized in all chromosome pericentromeric regions but some blocks could be visualized on some large chromosomes arms. A large heterozygous DA-CMA3-positive band was observed on one large chromosome arm, but was completely absent when C banding was applied before fluorochrome staining, with only one small positive band being visualized. Sequential DA-CMA3-NOR staining of interphase nuclei provided coincident positive responses. This suggests that DA-CMA3-positive bands of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meliponini; Partamona; Karyotype; C-banding; Fluorochromes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100009

Karyotypes of two cytotypes of Paspalum quadrifarium Lam. (Poaceae): an alternative technique for small chromosomes in plants
Provedor de dados: Genetics and Molecular Biology
Autores: Speranza,Pablo; Vaio,Magdalena; Mazzella,Cristina.
Paspalum quadrifarium Lam. is a bunchgrass native to Uruguay, Argentina, and southern Brazil. Diploid, triploid, tetraploid and hexaploid cytotypes have been reported for this species of the Quadrifaria group of Paspalum. In this group, a high degree of cytogenetic homology between the genomes of several diploid species has been reported, based on meiotic pairing in interspecific hybrids; multivalent associations would thus be expected in polyploid hybrids. Karyotype analysis could provide useful information about the genomic architecture of polyploid plants; however, the fully condensed mitotic chromosomes of Paspalum do not provide enough morphological features for such an analysis. In this paper, we used mitotic prometaphase chromosomes treated with 70%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Meiosis; Prometaphase; Grasses; Paspalum.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400013

Cytogenetics of two hylid frogs from Brazilian Cerrado
Provedor de dados: Genetics and Molecular Biology
Autores: Targueta,Cíntia Pelegrineti; Guerra,Vinícius; Gambale,Priscilla Guedes; Bastos,Rogério Pereira; Silva,Daniela de Melo e; Telles,Mariana Pires de Campos.
Abstract Cytogenetic data can be useful for taxonomic and phylogenetic studies, as well as to provide information about chromosome evolution. Therefore, it may help design conservation priorities for some threatened species, such as anurans. Herein, we describe the karyotypes of Scinax constrictus and Ololygon centralis, native endemic species from the Brazilian Cerrado. Chromosome preparations for both species were stained with Giemsa for morphological analyses and then impregnated by the Ag-NOR method for localization of the nucleolar organizer region (NOR). Both species had 24 chromosomes, as confirmed by meiotic analyses, which showed 12 bivalents. Chromosome morphologies presented the same pattern for Scinax and Ololygon compared to species already...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anura; Karyotype; Ololygon; Scinax.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500814

Description of the karyotype of Rhagomys rufescens Thomas, 1886 (Rodentia, Sigmodontinae) from Southern Brazil Atlantic forest
Provedor de dados: Genetics and Molecular Biology
Autores: Testoni,André Filipe; Althoff,Sérgio Luiz; Nascimento,André Paulo; Steiner-Souza,Francisco; Sbalqueiro,Ives José.
Rhagomys rufescens (Rodentia: Sigmodontinae) is an endemic species of the Atlantic forest from Southern and Southeastern Brazil. Some authors consider Rhagomys as part of the tribe Thomasomyini; but its phylogenetic relationships remain unclear. Chromosomal studies on eight specimens of Rhagomys rufescens revealed a diploid number of 2n = 36 and a number of autosome arms FN = 50. GTG, CBG and Ag-NOR banding and CMA3/DAPI staining were performed on metaphase chromosomes. Eight biarmed and nine acrocentric pairs were found in the karyotype of this species. The X and Y chromosomes were both acrocentric. Most of the autosomes and the sex chromosomes showed positive C-bands in the pericentromeric region. The X chromosome showed an additional heterochromatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhagomys rufescens; Thomasomyini; Rodentia; Atlantic forest; Karyotype.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300017

Comparative karyotype analysis of populations in the Alstroemeria presliana Herbert (Alstroemeriaceae) complex in Chile
Provedor de dados: Genetics and Molecular Biology
Autores: Baeza,Carlos; Finot,Víctor L.; Ruiz,Eduardo.
Alstroemeria L., one of the most diverse genera of the Chilean flora and of high floricultural value, is represented by 35 species, most of them distributed between 28–38° S in the Mediterranean zone of Central Chile. There are 24 complex-forming taxa, of which 18 have conservation problems (8 are considered “endangered” and 10 as “vulnerable”). One of these complexes is Alstroemeria presliana Herb. with two subspecies: subsp. presliana and subsp. australis Bayer. Alstroemeria presliana grows in Chile and Argentina: subsp. presliana is distributed from Reserva Nacional Siete Tazas (35°27′ S, Region of Maule) to Antuco, (37°25′ S, Region of Bío-Bío), and is also found in Neuquén, Argentina; subsp. australis is endemic to the Cordillera of Nahuelbuta. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alstroemeria; Asymmetry; Chile; Karyotype; Species complex.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200199

Chromosomal similarity between the Scaly-headed parrot (Pionus maximiliani), the Short-tailed parrot (Graydidascalus brachyurus) and the Yellow-faced parrot (Salvatoria xanthops) (Psittaciformes: Aves): a cytotaxonomic analysis
Provedor de dados: Genetics and Molecular Biology
Autores: Caparroz,Renato; Duarte,José Maurício Barbanti.
Behavior, morphology, allozyme studies and DNA hybridization and sequencing data all suggest the independent evolution of the Old and New World parrots and support tribe status for the American species, although the phylogenetic relationships within this tribe are still poorly understood. A previous study has shown that the Yellow-faced parrot (Amazona xanthops Spix 1824) exhibits large karyotypic differences compared to the other Amazona species and suggested that this species should be renamed Salvatoria xanthops, although the relationships between S. xanthops and the other New World parrots remain unclear. In the present work, we describe the karyotype of the Scaly-headed parrot (Pionus maximiliani, Kuhl 1820) and the karyotype and C-banding pattern of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome; Karyotype; Cytotaxonomy; Psittaciformes; Parrots.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400010

Meiotic chromosomes and nucleolar behavior in testicular cells of the grassland spittlebugs Deois flavopicta, Mahanarva fimbriolata and Notozulia entreriana (Hemiptera, Auchenorrhyncha)
Provedor de dados: Genetics and Molecular Biology
Autores: Castanhole,Márcia Maria Urbanin; Pereira,Luis Lênin Vicente; Souza,Hederson Vinicius de; Valério,José Raul; Barbosa,Leonardo Rodrigues; Itoyama,Mary Massumi.
Spittlebugs annually infest pastures and cause severe damage, representing a serious problem for the tropical American beef cattle industry. Spittlebugs are an important biotic constraint to forage production and there is a lack of cytogenetic data for this group of insects. For these reasons, we conducted this work, in which the spermatogenesis and nucleolar behavior of Deois flavopicta, Mahanarva fimbriolata and Notozulia entreriana were studied. The males possessed testes in the shape of a "bunch of grapes"; a variable number of testicular lobes per individual and polyploid nuclei composed of several heteropycnotic bodies. A heteropycnotic area was located in the periphery of the nucleus (prophase I); the chiasmata were terminal or interstitial;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal characterization; Karyotype; Cytogenetics; Spermatogenesis; Holocentric chromosomes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200008

Nuclear DNA content and karyotype of Rosewood (Aniba rosaeodora)
Provedor de dados: Genetics and Molecular Biology
Autores: Contim,Luis Antônio Serrão; Carvalho,Carlos Roberto de; Martins,Franciele Alline; Freitas,Danival Vieira de.
Rosewood (Aniba rosaeodora Ducke, Lauraceae) is ecologically and economically important to the Amazon region. As a consequence of its economic importance, rosewood populations have been decimated in the Amazon forest. Species of nine genera of the Lauraceae family have characterized karyotypes with n = x = 12 chromosomes in the gametophytic phase but the genus Aniba is one of the least studied Lauraceae genera with a previously undescribed genome. We used cytogenetic techniques to determine that the A. rosaeodora karyotype contained 12 pairs (2n = 24) of relatively small submetacentric chromosomes with lengths ranging from 1.34 to 2.25 mum and a nucleolar organizer region (NOR) in the short arm of chromosome 7. Flow cytometry gave 2C = 2.32 pg of DNA,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosewood; Aniba rosaeodora; Lauraceae; Karyotype; Flow cytometry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500017

Cytogenetic studies of three Lycosidae species   from Argentina (Arachnida, Araneae)
Provedor de dados: Genetics and Molecular Biology
Autores: Chemisquy,María A.; Gil,Sergio G. Rodríguez; Scioscia,Cristina L.; Mola,Liliana M.
Cytogenetic studies of the family Lycosidae (Arachnida: Araneae) are scarce. Less than 4% of the described species have been analyzed and the male haploid chromosome numbers ranged from 8+X 1  X  2  to 13+X 1  X  2 . Species formerly classified as  Lycosa  were the most studied ones. Our aim in this work was to perform a comparative analysis of the meiosis in " Lycosa "  erythrognatha  Lucas, " Lycosa "  pampeana  Holmberg and  Schizocosa malitiosa  (Tullgren). We also compared male and female karyotypes and characterized the heterochromatin of " L. "  erythrognatha . The males of the three species had 2n = 22, n = 10+X 1 X 2 , all the chromosomes were telocentric and there was generally a single chiasma per bivalent. In " Lycosa "  pampeana , which is...
Palavras-chave: Meiosis; C-banding; Fluorochrome staining; Karyotype; Spiders; "Lycosa"  and Schizocosa.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500010

Cytogenetic comparison of Podocnemis expansa and Podocnemis unifilis: a case of inversion and duplication involving constitutive heterochromatin
Provedor de dados: Genetics and Molecular Biology
Autores: Gunski,Ricardo José; Cunha,Isabel Souza; Degrandi,Tiago Marafiga; Ledesma,Mario; Garnero,Analía Del Valle.
Podocnemis expansa and P. unifilis present 2n = 28 chromosomes, a diploid number similar to those observed in other species of the genus. The aim of this study was to characterize these two species using conventional staining and differential CBG-, GTG and Ag-NOR banding. We analyzed specimens of P. expansa and P. unifilis from the state of Tocantins (Brazil), in which we found a 2n = 28 and karyotypes differing in the morphology of the 13th pair, which was submetacentric in P. expansa and telocentric in P. unifilis. The CBG-banding patterns revealed a heterochromatic block in the short arm of pair 13 of P. expansa and an interstitial one in pair 13 of P. unifilis, suggesting a pericentric inversion. Pair 14 of P. unifilis showed an insterstitial band in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome banding; Ag-NORs; Karyotype; Chelonids.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300009

Karyotypic analyses and morphological comments on the endemic and endangered Brazilian painted tree rat Callistomys pictus (Rodentia, Echimyidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Ventura,Karen; Ximenes,Gilson Evaristo Iack; Pardini,Renata; Sousa,Marcos A. Nóbrega de; Yonenaga-Yassuda,Yatiyo; Silva,Maria José de J.
The genus Callistomys belongs to the rodent family Echimyidae, subfamily Echimyinae, and its only living representative is Callistomys pictus, a rare and vulnerable endemic species of the state of Bahia, Brazil. Callistomys has been previously classified as Nelomys, Loncheres, Isothrix and Echimys. In this paper we present the karyotype of Callistomys pictus, including CBG and GTG-banding patterns and silver staining of the nucleolus organizer regions (Ag-NORs). Comments on Callistomys pictus morphological traits and a compilation of Echimyinae chromosomal data are also included. Our analyses revealed that Callistomys can be recognized both by its distintinctive morphology and by its karyotype.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Callistomys; Karyotype; Banding patterns; Echimyinae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400016

Cytogenetic analysis and chromosomal characteristics of the polymorphic 18S rDNA in the fish Prochilodus lineatus (Characiformes, Prochilodontidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Vicari,Marcelo Ricardo; Almeida,Mara Cristina de; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando; Artoni,Roberto Ferreira.
We used differential staining techniques (BSG, GTG, AgNO3, DAPI and CMA3 banding) and fluorescent in situ hybridization (FISH) with 5S and 18S probes to investigated the karyotypic and cytogenetic chracteristics of Prochilodus lineatus specimens from a population in Vila Velha state park (Parque Estadual de Vila Velha, Ponta Grossa, Paraná state, southern Brazil). The specimens studied showed the same karyotype as that found in other P. lineatus populations, i.e. 2n = 54 biarmed chromosomes (40m + 14 sm) and c-positive heterochromatin preferentially located pericentromerically in all chromosomes. The presence of partial or totally heterochromatic supernumerary chromosomes with numeric intra-individual variation was confirmed in the analyzed population. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish cytogenetics; Prochilodus; Karyotype; Minor and major rDNA; NOR polymorphism.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400008

Cytogenetic analysis of three catfish species of the family Pseudopimelodidae (Teleostei, Siluriformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Martinez,Emanuel Ricardo Monteiro; Nirchio,Mauro; Granado,Angel; Foresti,Fausto; Oliveira,Claudio.
Cytogenetic analyses performed in Cephalosilurus apurensis, Microglanis aff. cottoides and Pseudopimelodus bufonius revealed that the three species have 2n = 54 chromosomes: C. apurensis presented six metacentric (M), 28 submetacentric (SM), 14 subtelolocentric (ST), and six acrocentric (A) chromosomes, while M. aff. cottoides showed 10M, 32SM, 10ST and 2A, and P. bufonius had 12M, 30SM and 12ST. The nucleolus organizer regions (NORs) were present on the short arm of a middle-sized ST pair, identified as pair 19, in C. apurensis NORs were found on the short arm of a middle-sized ST (pair 23) and on the long arm of a middle-sized ST (pair 22) in M. aff. cotttoides and on the short arm of three middle-sized ST pairs, identified as pairs 9, 10 and 11, in P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Chromosomes; C-banding; Ag-NOR; Fish.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400015

Detection of diploid males in a natural colony of the cleptobiotic bee Lestrimelitta sp (Hymenoptera, Apidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Tavares,Mara Garcia; Carvalho,Carlos Roberto; Soares,Fernanda Aparecida Ferrari; Fernandes,Anderson.
When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to be diploid, since, through cytometric analysis, it was demonstrated that the mean genome size of both males and females was the same (C = 0.463 pg), and, furthermore, cytogenetic analysis demonstrated that both had 2n = 28 chromosomes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetic; Flow cytometry; Genome size; Karyotype; Stingless bee.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300019

Mapping of ribosomal genes and chromosomal markers in three species of the genus  Serrasalmus  (Characidae, Serrasalminae) from the Amazon basin
Provedor de dados: Genetics and Molecular Biology
Autores: Nakayama,Celeste M.; Feldberg,Eliana; Bertollo,Luiz Antonio C.
Karyotypic characteristics of three species of the genus  serrasalmus  ( S. altispinnis ,  S. gouldingi  and  S. Serrulatus ) from the middle and lower Negro River, Amazon Basin, were investigated using different staining techniques and Fluorescent  in situ  hybridization with 5S and 18S rDNA probes. The diploid number was invariably 2n = 60 and the fundamental number was FN = 110. Nevertheless, the karyotypes differed from each other in composition: 24m, 20sm, 6st, 10a in  S. altispinnis ; 22m, 22sm, 6st, 10a in  S. gouldingi  and 20m, 22sm, 8st, 10a in  S. serrulatus.  The karyotype of  S. altispinnis  differed from the one previously described in a population from the Pitinga River. C-positive constitutive heterochromatin was mainly pericentromeric in...
Palavras-chave: Chromosome banding; Cytotaxonomy of piranhas; FISH; Karyotype; Ribosomal DNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500011

Esterase-D and chromosome patterns in Central Amazon piranha (Serrasalmus rhombeus Linnaeus, 1766) from Lake Catalão
Provedor de dados: Genetics and Molecular Biology
Autores: Teixeira,Aylton Saturnino; Nakayama,Celeste Mutuko; Porto,Jorge Ivan Rebelo; Feldberg,Eliana.
This study presents additional genetic data on piranha (Serrasalmus rhombeus Linnaeus, 1766) complex previously diagnosed due to the presence of distinct cytotypes 2n = 58 and 2n = 60. Three esterase-D enzyme loci (Est-D1, Est-D2 and Est-D3) were examined and complemented with chromosomal data from 66 piranha specimens collected from Lake Catalão. For all specimens the Est-D1 and Est-D2 loci were monomorphic. In contrast, the Est-D3 locus was polymorphic with genotypes and alleles being differentially distributed in the previously described cytotypes and served as the basis for detecting a new cytotype (2n = 60 B). In cytotype 2n = 58 the Est-D3 locus was also polymorphic and presented Mendelian allelic segregation with four genotypes (Est-D3(11),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian Amazon basin; Esterase enzymes; Serrasalmus rhombeus species complex; Karyotype.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300018

Comparative cytogenetics of Hoplias malabaricus (Pisces, Erythrinidae): a population analysis in adjacent hydrographic basins
Provedor de dados: Genetics and Molecular Biology
Autores: Vicari,Marcelo Ricardo; Artoni,Roberto Ferreira; Bertollo,Luiz Antonio Carlos.
The chromosomes of specimens from four Hoplias malabaricus populations from headwaters of adjacent river basins at Ponta Grossa, southern Brazil, were investigated using differential staining techniques (C-banding, AgNO3 and CMA3) and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. The diploid chromosome number in representatives of all four populations was invariably 2n = 42, with karyotypes composed of 12 pairs of metacentrics and 9 pairs of submetacentrics, without heteromorphic sex chromosomes. This kind of karyotype represents cytotype A in regard to cytotypes identified previously in H. malabaricus, exhibiting however, at the same time, some differences in the distribution of constitutive heterochromatin segments and in the locations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome banding; FISH; Cytotaxonomy of trahiras; Karyotype; NOR phenotype.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100018

Cytogenetic and morphological diversity in populations of Astyanax fasciatus (Teleostei, Characidae) from Brazilian northeastern river basins
Provedor de dados: Genetics and Molecular Biology
Autores: Medrado,Aline Souza; Figueiredo,Alba Vivian Amaral; Waldschmidt,Ana Maria; Affonso,Paulo Roberto Antunes de Mello; Carneiro,Paulo Luiz Souza.
In the present work, morphometric and cytogenetic analyses were carried out in populations of the fish Astyanax fasciatus (Characidae) from Contas and Recôncavo Sul River basins (State of Bahia, Brazil), providing new data on the genetic structure of this species along the region. Based on morphologic measurements, we observed that populations from the same hydrographic basin were more similar to each other (Contas and Preto do Costa Rivers), and remarkably divergent from Recôncavo Sul (Mineiro Stream), as indicated by clustering analysis. Cytogenetic data revealed a same diploid number for all populations (2n = 48), but distinct karyotype formulae (8M+24SM+12ST+4A, FN = 92 in Contas River, 8M+24SM+10ST+6A, FN = 90 in Preto do Costa River, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canonic variables; Karyotype; Morphometry; NOR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200007

The karyotype of the critically endangered Lear's macaw, Anodorhynchus leari Bonaparte 1856 (Aves, Psittaciformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Nogueira,Denise Monnerat; Souza,Lucia Moreno de; Goldschmidt,Beatriz; Silva,Christiano Pinheiro da; Monsores,Denise Wilches.
We used conventional chromosomal staining to describe the karyotype of the critically endangered Lear's macaw, Anodorhynchus leari Bonaparte 1856. A diploid number of 2n = 70 and a karyotype similar to that of its congener Anodorhynchus hyacinthinus suggests that chromosomal rearrangements were not the main evolutionary mechanism in the genus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birds; Karyotype; Chromosomes; Psittaciformes; Endangered species.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400014

Karyotype, C-and fluorescence banding pattern, NOR location and FISH study of five Scarabaeidae (Coleoptera) species
Provedor de dados: Genetics and Molecular Biology
Autores: Bione,Edgar; Moura,Rita de Cássia de; Carvalho,Reginaldo de; Souza,Maria José de.
Meiotic chromosomes obtained from members of the coleopteran subfamilies Rutelinae and Dynastinae were studied using standard and silver nitrate staining, C-banding, base-specific fluorochromes and fluorescent in situ hybridization (FISH). The study presents detailed karyotipic descripitions of three Rutelinae species (Geniates borelli, Macraspis festiva and Pelidnota pallidipennis), and two Dynastinae species (Lygirus ebenus and Strategus surinamensis hirtus) with special emphasis on the distribution and variability of constitutive heterochromatin and the nucleolar organizer region (NOR). We found that for G. borelli, P. pallidipennis, L. ebenus and S. s hirtus the karyotype was 2n = 20 (9II + Xy p), with G. borelli, P. pallidipennis and L. ebenus showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Constitutive heterochromatin; NORs; RDNA sequences.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300006

Description of the karyotypes of Stejneger's beaked whale (Mesoplodon stejnegeri) and Hubbs’ beaked whale (M. carlhubbsi)
Provedor de dados: Genetics and Molecular Biology
Autores: Kurihara,Nozomi; Tajima,Yuko; Yamada,Tadasu K.; Matsuda,Ayaka; Matsuishi,Takashi.
Abstract The genus Mesoplodon (Cetacea: Odontoceti: Ziphiidae) is one of the few cetacean genera with the karyotype 2n = 42. The 2n = 42 karyotype of M. europaeus and M. carlhubbsi is largely consistent with the general cetacean karyotype 2n = 44, although other 2n = 42 karyotypes do not exhibit clear homologies with the general cetacean karyotype. Therefore, the chromosomes of Mesoplodon species may be the key to understanding cetacean karyological evolution. In the present study, the male karyotypes of M. stejnegeri and M. carlhubbsi were examined. In both species, the diploid number of the male karyotype was 42. Both species had the following characteristics: 1) a huge subtelocentric X chromosome with a large C-block; 2) a small metacentric Y...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Chromosome; Mesoplodon stejnegeri; Mesoplodon carlhubbsi.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500803

Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera)
Provedor de dados: Genetics and Molecular Biology
Autores: Costa,Laiana Cristina da; Azeredo-Oliveira,Maria Tercília Vilela de; Tartarotti,Ester.
Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triatoma klugi; Triatomine; Cytogenetics; Nucleolus; Karyotype; Meiosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300008

Karyotype and nuclear DNA content of Trichomycterus areolatus (Siluriformes, Trichomycteridae)
Provedor de dados: Genetics and Molecular Biology
Autores: Colihueque,Nelson; Corrales,Olga; Parraguez,Margarita.
Cytogenetic analysis of Trichomycterus areolatus, collected from the Tijeral and Huilma Rivers in southern Chile has shown a diploid chromosome number of 2n = 54, a fundamental number of FN = 106, and a karyotypic formula of 44m + 8sm + 2st. Intra-individual polymorphism of chromosome number (2n = 54, 55 and 56) in specimens from the Huilma River has also been documented, providing further evidence of the occurrence of this phenomenon in Trichomycterus. The karyotype exhibited large chromosome pairs: metacentric pairs 1 (relative length 7.54%), 2 (5.75%) and 3 (5.09%), submetacentric pair 23 (5.25%), and subtelocentic pair 27 (5.28%). Nuclear DNA content analysis showed an average value of 5.04 ± 1.09 pg/nucleus. This DNA content is higher than the mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Nuclear DNA content; Trichomycterus; Siluriformes.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200014

Multiple interstitial ribosomal sites (NORs) in the Brazilian squirrel Sciurus aestuans ingrami (Rodentia, Sciuridae) with 2n = 40: an overview of Sciurus cytogenetics
Provedor de dados: Genetics and Molecular Biology
Autores: Fagundes,Valéria; Christoff,Alexandre Uarth; Amaro-Ghilard,Renata Cecília; Scheibler,Daniel R.; Yonenaga-Yassuda,Yatiyo.
This is the first time the karyotype of the Brazilian squirrel Sciurusaestuans ingrami, with 2n = 40, is described. The karyotype of this species comprises 18 pairs of biarmed and one minute pair of acrocentric autosomes, a medium-sized submetacentric X and a medium-sized acrocentric Y. Four pairs have an interstitial secondary constriction, co-located with nucleolar organizer regions (NORs), identified by silver-staining technique and fluorescent in situ hybridization (FISH) with ribosomal 18S/28S probes. The occurrence of multiple interstitial NORs is rare in rodents, and this is one of the few examples, identified by a molecular cytogenetics approach.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Squirrels; Chromosomes; Karyotype; NORs; Rodentia.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300007

Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation
Provedor de dados: Genetics and Molecular Biology
Autores: Mittmann,Josane; Kerkis,Irina; Kawashima,Cinthia; Sukoyan,Marina; Santos,Enrico; Kerkis,Alexandre.
We studied the karyotypes of three hybrid clones of mouse embryonic stem cells and murine splenocytes (two having near diploid and one having near tetraploid chromosome numbers) and the characteristics of their differentiation during the formation of embryoid bodies. The X chromosome originating from embryonic stem cells may be lost in hybrids with a near diploid chromosome number and reprogramming of the "somatic" X may occur. The morphological data we obtained using light and electron microscopy revealed a correlation between the karyotype constitution of hybrid cells and their differentiation during the formation of embryoid bodies. At the beginning of development, the embryoid bodies derived from hybrid cells already showed an advanced degree of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mouse ES-somatic cell hybrids; Karyotype; Pluriopotency; Differentiation; Embryoid bodies.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100019

Cytotaxonomic study of the Chilean endemic complex Alstroemeria magnifica Herb. (Alstroemeriaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Baeza,Carlos M.; Finot,Víctor; Ruiz,Eduardo; Carrasco,Pedro; Novoa,Patricio; Rosas,Marcelo; Toro-Núñez,Oscar.
Abstract Alstroemeria L. (Alstroemeriaceae) represents one of the most diverse genera of vascular plants in Chile. It contains approximately 54 taxa, 40 of which are endemic. The “complex” Alstroemeria magnifica is endemic to Chile, and it comprises four varieties: A. magnifica var. magenta, A. magnifica var. magnifica, A. magnifica var. sierrae, and A. magnifica var. tofoensis. It is distributed from Coquimbo to the Valparaíso Region. We analyzed karyotypes of 10 populations along its natural distribution. All the populations presented an asymmetric karyotype, with 2n = 16 chromosomes but with three different karyotypic formulae. Alstroemeria magnifica var. magnifica and A. magnifica var. sierrae presented the same karyotypic fomula, and A. magnifica var....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alstroemeria; Karyotype; Species complex; Asymmetry; Chile.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300434

Cytotaxonomy and karyoevolution of the genus Crenicichla (Perciformes, Cichlidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Benzaquem,Denise Corrêa; Feldberg,Eliana; Porto,Jorge Ivan Rebelo; Gross,Maria Claudia; Zuanon,Jansen Alfredo Sampaio.
Karyotypes of six cichlid species of the genus Crenicichla were investigated. The species C. cincta, C. inpa, C. reticulata, C. lugubris, and C. cf. johanna were collected from Amazon basin, and C. britskii was collected from the Paraná-Paraguai basin. All of the analysed species showed 2n = 48 chromosomes; however, C. cincta, C. lugubris, C. cf. johanna, and C. britskii had a karyotype formula of 8M-SM+40ST-A, FN = 56, while C. inpa and C. reticulata exhibited the formula 6M-SM+42ST-A, FN = 54. Analysis of active Ag-NORs revealed two NOR-bearing chromosomes in all species; however, theses cistrons were located on different chromosome pairs and/or in different chromosome locations in each species. This condition bears evolutionary significance, since it is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Pike cichlid; Chromosome marker; Amazon.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200016

Genetic identification of bucktooth parrotfish Sparisoma radians (Valenciennes, 1840) (Labridae, Scarinae) by chromosomal and molecular markers
Provedor de dados: Genetics and Molecular Biology
Autores: Paim,Fabilene Gomes; Brandão,José Henrique Souza Galdino; Sampaio,Iracilda; Affonso,Paulo Roberto Antunes de Mello; Diniz,Débora.
Parrotfishes (Labridae, Scarinae) comprise a large marine fish group of difficult identification, particularly during juvenile phase when the typical morphology and coloration of adults are absent. Therefore, the goal of this study was to test cytogenetic markers and DNA barcoding in the identification of bucktooth parrtotfish Sparisoma radians from the northeastern coast of Brazil. Sequencing of cytochrome c oxidase subunit I (COI) confirmed all studied samples as S. radians, and all showed high similarity (99-100%) with Caribbean populations. The karyotype of this species was divergent from most marine Perciformes, being composed of 2n = 46 chromosomes. These consisted of a large number of metacentric and submetacentric pairs with small amounts of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COI; Karyotype; South Atlantic fish; Ribossomal genes; Robertsonian rearrangements.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500007

Novel insights into chromosome evolution of Charadriiformes: extensive genomic reshuffling in the wattled jacana (Jacana jacana, Charadriiformes, Jacanidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Kretschmer,Rafael; Souza,Marcelo Santos de; Barcellos,Suziane Alves; Degrandi,Tiago Marafiga; Pereira,Jorge C.; O’Brien,Patricia C.M.; Ferguson-Smith,Malcolm A.; Gunski,Ricardo José; Garnero,Analía del Valle; Oliveira,Edivaldo Herculano Correa de; Freitas,Thales Renato Ochotorena de.
Abstract The order Charadriiformes comprises three major clades: Lari and Scolopaci as sister group to Charadrii. Until now, only three Charadriiformes species have been studied by chromosome painting: Larus argentatus (Lari), Burhinus oedicnemus and Vanellus chilensis (Charadrii). Hence, there is a lack of information concerning the third clade, Scolapaci. Based on this, and to gain a better understanding of karyotype evolution in the order Charadriiformes, we applied conventional and molecular cytogenetic approaches in a species belonging to clade Scolopaci - the wattled jacana (Jacana jacana) - using Gallus gallus and Zenaida auriculata chromosome-specific probes. Cross-species evaluation of J. jacana chromosomes shows extensive genomic reshuffling...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Charadrii; Karyotype; Avian genome; Comparative mapping.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100601

Karyotype and nuclear DNA content of hexa-, octo-, and duodecaploid lines of Bromus subgen. Ceratochloa
Provedor de dados: Genetics and Molecular Biology
Autores: Klos,Joanna; Sliwinska,Elwira; Kula,Adam; Golczyk,Hieronim; Grabowska-Joachimiak,Aleksandra; Ilnicki,Tomasz; Szostek,Krzysztof; Stewart,Alan; Joachimiak,Andrzej J.
The subgenus Ceratochloa of the genus Bromus includes a number of closely related allopolyploid forms or species that present a difficult taxonomic problem. The present work combines data concerning chromosome length, heterochromatin distribution and nuclear genome size of different 6x, 8x and 12x accessions in this subgenus. Special attention is paid to the karyotype structure and genomic constitution of duodecaploid plants recently found in South America. Hexaploid lineages possess six almost indistinguishable genomes and a nuclear DNA content between 12.72 pg and 15.10 pg (mean 1Cx value = 2.32 pg), whereas octoploid lineages contain the same six genomes (AABBCC) plus two that are characterized by longer chromosomes and a greater DNA content (1Cx = 4.47...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; C-banding; Heterochromatin; Flow cytometry; Genome size.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300016

Cytogenetics of three Brazilian species of Eleutherodactylus (Anura, Leptodactylidae) with 22 chromosomes and re-analysis of multiple translocations in E. binotatus
Provedor de dados: Genetics and Molecular Biology
Autores: Siqueira-Jr.,Sérgio; Ananias,Fernando; Recco-Pimentel,Shirlei M.
In this paper, we provide a cytogenetic analysis of Eleutherodactylus guentheri, E. parvus and E. binotatus. All of the species had a diploid chromosomal number of 2n = 22. The karyotypes of E. guentheri and E. parvus were very similar and differed only slightly in the morphology of pair 2. These two species also had an NOR-bearing secondary constriction on the long arms of pair 6. The karyotype of E. binotatus differed from those of E. guentheri and E. parvus in the morphology and size of the chromosomes, in the number of chromosomal arms, in the NOR location (detected on the short arms of pair 1), and in the pattern of heterochromatin. These results reinforce the differences between E. guentheri and E. binotatus and support the existence of two species...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eleutherodactylus; Heterochromatin; Karyotype; Nucleolus organizing region (NOR); Reciprocal translocation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300010

Chromosome mapping of 5S rRNA genes differentiates Brazilian populations of Leporellus vittatus (Anostomidae, Characiformes)
Provedor de dados: Genetics and Molecular Biology
Autores: Aguilar,Cecilia Teixeira de; Galetti Junior,Pedro Manoel.
Among the anostomid fishes, the genus Leporellus is represented by only three species: L. nattereri, endemic of the Amazon River, L. retropinnis, endemic of the Piracicaba River, and L. vittatus, widely distributed in rivers from Peru, Colombia, Guianas, and different major hydrographic basins of Brazil. A cytogenetic study carried out on specimens of Leporellus vittatus from three major Brazilian hydrographic basins evidenced a karyotype of 54 metacentric and submetacentric chromosomes. C-banding analysis revealed the presence of large pericentromeric heterochromatic segments in all chromosomes and a telomeric block coincident with the NOR sites. Ag, CMA3 or MM staining, and FISH with ribosomal probes located the 45S ribosomal genes on the terminal region...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; C-bands; 45S rDNA; 5S rDNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200004

Comparative karyotypic analysis in the Alstroemeria hookeri Lodd. (Alstroemeriaceae) complex sensu Bayer (1987)
Provedor de dados: Genetics and Molecular Biology
Autores: Baeza,Carlos; Ruiz,Eduardo; Negritto,María.
Alstroemeria L. (Alstroemeriaceae) is an American genus of monocots with two principal distribution centers in Chile and Brazil. In Chile, it is represented by about 32 species, most of them in central Chile, an area known for its high level of endemism. The "complex" Alstroemeria hookeri is endemic to Chile, where it is distributed from the Coquimbo to the Bío-Bío Region. We analyzed the karyotypes of 36 populations of this complex along its natural distribution. Ten metaphases per population were used for chromosome measurements. All analyzed subspecies presented a well defined asymmetric karyotype. The populations of A. hookeri subsp. hookeri collected in the coastal range of the Bío-Bío Region and the populations from the Central Valley of this Region...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alstroemeria hookeri; Complex; Karyotype; Polymorphisms; Chile.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100020

Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Guilherme Messias da; Bione,Edgar Guimarães; Cabral-de-Mello,Diogo Cavalcanti; Moura,Rita de Cássia de; Simões,Zilá Luz Paulino; Souza,Maria José de.
Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy p type in D. nisus and D. semisquamosus and of the Xyr type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome rearrangements; Heterochromatin; Karyotype; NORs; FISH.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200012

Chromatin differentiation between Theobroma cacao L. and T. grandiflorum Schum
Provedor de dados: Genetics and Molecular Biology
Autores: Dantas,Liliane G.; Guerra,Marcelo.
A comparative analysis of mitotic chromosomes of Theobroma cacao (cacao) and T. grandiflorum (cupuaçu) was performed aiming to identify cytological differences between the two most important species of this genus. Both species have symmetric karyotypes, with 2n = 20 metacentric chromosomes ranging in size from 2.00 to 1.19 µm (cacao) and from 2.21 to 1.15 mm (cupuaçu). The interphase nuclei of both species were of the arreticulate type, displaying up to 20 chromocentres, which were more regularly shaped in cacao than in cupuaçu. Prophase chromosomes of both species were more condensed in the proximal region, sometimes including the whole short arm. Both species exhibited only one pair of terminal heterochromatic bands, positively stained with chromomycin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cacao; Cupuaçu; Heterochromatin; Karyotype; RDNA sites; Theobroma.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100017

Cytogenetic study in natural hybrids of Callithrix (Callitrichidae: Primates) in the Atlantic forest of the state of Rio de Janeiro, Brazil
Provedor de dados: Iheringia Série Zoologia
Autores: Nogueira,Denise M.; Ferreira,Ana Maria R.; Goldschmidt,Beatriz; Pissinatti,Alcides; Carelli,Juliane B.; Verona,Carlos E.
In the Atlantic forest of Rio de Janeiro, Callithrix aurita (É. Geoffroy in Humboldt, 1812) is a native species vulnerable to extinction and C. jacchus (Linnaeus, 1758) and C. penicillata (É. Geoffroy, 1812) are invasive species. The major threats to the native species are habitat degradation and hybridization, although there are currently no genetic data about natural hybrids available. Previous studies have revealed that species of the Callithrix genus are extremely homogeneous in their karyotypes with the exceptions of the morphology and size of the Y chromosome and its nucleolar organizer region (NOR) banding pattern. Three male marmosets captured in the wild in Guapimirim municipality, Rio de Janeiro, Brazil, considered as possible hybrids between C....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marmosets; Y-chromosome; Karyotype; Hybridization.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212011000200002

A new karyotype of Calomys (Rodentia, Sigmodontinae)
Provedor de dados: Iheringia Série Zoologia
Autores: Lima,J. Fernando de S.; Kasahara,Sanae.
The genus Calomys Waterhouse, 1837 is widely distributed within South America, being found in Venezuela, Colombia, Peru, Bolivia, Brazil, Paraguay, Uruguay and Argentina. Specimens of Calomys were collected in Formoso do Araguaia, Tocantins, Brazil. For chromosome characterization standard staining techniques and as G-banding and nucleolar organizer region were used. The karyotype was 2n=46 and AN=66. The X chromosome is a medium metacentric and the Y chromosome a small acrocentric chromosome. Chromosome homologies with other species were observed. Probably, karyotype differences were basically due to Robertsonian rearrangements.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calomys; Karyotype; Chromosome; Tocantins; Brazil.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212001000200019

Comparative cytogenetics in different populations of the cavernicolous diplopod Pseudonannolene strinatii (Diplopoda, Pseudonannolenidae)
Provedor de dados: Iheringia Série Zoologia
Autores: Campos,Kleber A.; Fontanetti,Carmem S.
Different populations of Pseudonannolene strinatii Mauriès, 1974 collected from three caves in Iporanga, state of São Paulo, were cytogenetically compared using techniques of conventional coloration, C-banding and silver nitrate impregnation. Specimens were morphologically similar and small cytogenetic differences were observed between the populations with relation to the distribution of constitutive heterochromatin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Millipedes; C-banding; Karyotype; AgNOR; Chromosomes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212013000100006

Comparative karyological study of cupped oyster species
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Leitao, Alexandra; Boudry, Pierre; Labat, Jean-philippe; Thiriot-quievreux, Catherine.
Chromosomes of six cupped oyster species were studied using karyometric analysis alter conventional Giemsa staining, and silver staining, Karyotypes of Crassotrea angulata (nine metacentric and one submetacentric chromosome pairs), C. sikamea (nine metacentric and one submetacentric chromosome pairs), C. virginica (eight metacentric and two submetacentric chromosome pairs), C, ariakensis (eight metacentric and two submetacentric chromosome pairs), C, gasar (six metacentric and four submetacentric chromosome pairs), and Saccostrea commercialis (eight metacentric and two submetacentric chromosome pairs) are distinguishable by the number and position of the submetacentric chromosome pair and by the location of nucleolus organizer regions, Comparative...
Tipo: Text
Palavras-chave: Bivalvia; Cytotaxonomy; Karyotype; Chromosome; Cupped oyster.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-2737.pdf

Evidence of differential chromosome loss in aneuploid karyotypes of the Pacific oyster, Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Leitao, Alexandra; Boudry, Pierre; Thiriot Quievreux, Catherine.
The G-banding technique was performed on aneuploid karyotypes from gill tissue of the Pacific oyster, Crassostrea gigas, to assess whether chromosome losses could be explained by differential chromosomal susceptibility and to clarify the negative correlation between aneuploidy and growth rate previously reported in different populations of this oyster. The study of 95 G-banded aneuploid karyotypes showed that only 4 of the 10 chromosome pairs (viz. 1, 5, 9, and 10) of C. gigas were affected by the loss of one homologous chromosome. Pairs 1, 9, and 10, which were lost in 56, 33, and 44% of cases, respectively, may be considered to be differentially affected. Hypotheses on this differential chromosomal susceptibility are discussed.
Tipo: Text
Palavras-chave: Oyster; Karyotype; G banding; Growth; Aneuploidy; Huître; Caryotype; Bandes G; Croissance; Aneuploïdie.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-742.pdf

Trans-Atlantic distribution of a mangrove oyster species revealed by 16S mtDNA and karyological analyses
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Heurtebise, Serge; Garcia, P; Thiriot Quievreux, C; Boudry, Pierre.
Three species of man.-rove oysters, Crassostrea rhizophorae, C. brasiliana, and C. gasar, have been described along the Atlantic shores of South America and Africa. Because the distribution of these molluscs is of great biological and commercial interest, their taxonomy and distribution deserve further clarification. Therefore, 15 populations were sampled from both continents. Their 16S mitochondrial polymorphism was studied by sequencing and PCR-RFLP analysis. Two haplotypes were identified. Haplotype a was the only one observed in Africa, but it was also observed in South America together with haplotype b. Because C. gasar is the only mangrove oyster identified on the west coast of Africa, haplotype a was attributed to this species, which has thus been...
Tipo: Text
Palavras-chave: Mangrove oyster; Crassostrea gasar; Karyotype; Phylogeography; 16S mitochondrial DNA.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-2790.pdf

Karyotype variation in neoplastic cells associated to severity of disseminated neoplasia in the cockle Cerastoderma edule
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Matias, A. M.; Diaz, S.; Teixeira De Sousa, Joana; Villalba, A.; Carballal, M. J.; Leitao, A.
Disseminated neoplasia (DN) is characterized by an abnormal cell proliferation, usually resulting in death of the affected mollusks. In the present study we performed, for the first time, karyotypes of two different stages of DN in the cockle Cerastoderma edule. Analysis of moderate (N2) and high (N3) severity neoplasic metaphases displayed a chromosome number ranging from 57 to 317, instead of the normal diploid number of 38. Cockles with N2 showed a lower percentage of microchromosomes and a higher ploidy than those with N3. The comparison of both cytogenetic profiles contributes to better understand the evolution of DN in cockles.
Tipo: Text
Palavras-chave: Karyotype; Disseminated neoplasia; Cerastoderma edule; Microchromosomes.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00184/29550/27876.pdf

Chilean plants cytogenetic database: coverage, features and usages
Provedor de dados: Journal of Basic and Applied Genetics (BAG)
Autores: Jara-Seguel,Pedro; Urrutia-Estrada,Jonathan.
Chilean Plants Cytogenetic Database (CPCD) is a resource available on line in electronic format, which provides a cytogenetics catalogue for continental and insular Chilean vascular plants. In this report, we made reference to the CPCD, discussing aspects on its coverage, features and usages. Currently, the database stores cytogenetic information for 247 species, 107 genera, and 55 families belonging to Pteridophyta, Pinophyta, and Magnoliophyta.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome number; Karyotype; Chilean Plants.
Ano: 2018
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332018000100006

Chromosomal characterization of Irenomys Tarsalis (Rodentia, Cricetidae, Sigmodontinae)
Provedor de dados: Mastozoología Neotropical
Autores: Ojeda,Agustina A.; Ríos,Carolina A.; Gallardo,Milton H.
The systematics of I. tarsalis is far from being understood. Morphological and molecular analyses have given contradictory results, pointing towards its inclusion or exclusion from the Phyllotini. The karyotype of Irenomys tarsalis, as well as its C-bands are reported for the first time. The species has 2n = 64 chromosomes (FN = 98) and a C-banding pattern characterized by small amounts of centromeric heterochromatin. Although the high diploid number and the C-bands of I. tarsalis support the notion of the species´ ancestral karyotypic condition, its affiliation within the sigmodontines cannot be discerned by the data.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Irenomys tarsalis; Karyotype; Sigmodontinae; Phyllotini.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-93832004000100011

Cytogenetics studies in thirteen Brazilian species of Phaneropterinae (Orthoptera: Tettigonioidea: Tettigoniidae): main evolutive trends based on their karyological traits
Provedor de dados: Neotropical Entomology
Autores: Ferreira,Amilton; Mesa,Alejo.
The thirteen species of Phaneropterinae here studied can be arranged in four different groups according to their basic karyological traits. All of them share the same kind of chromosomal sex determining mechanism with X0(male) and XX(female). The X chromosome differs among species and always appears heteropycnotic during prophase I, it is the largest in the set and segregates precociously during anaphase I. Among the species, the karyotypes varies in fundamental number between 31 to 21. The meaning of these significant changes in the karyotypes in relation to the phylogeny within some large taxonomic group of species is discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Chromosome; Evolution.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2007000400003

Cytogenetic studies in three species of Lutjanus (Perciformes: Lutjanidae: Lutjaninae) from the Isla Margarita, Venezuela
Provedor de dados: Neotropical Ichthyology
Autores: Nirchio,Mauro; Rondón,Rodolfo; Oliveira,Claudio; Ferreira,Irani A.; Martins,Cesar; Pérez,Julio; Sola,Luciana; Rossi,Anna Rita.
In the present study, three species of Lutjaninae, Lutjanus analis, L. griseus and L. synagris, were analyzed by conventional Giemsa staining, C-banding and silver staining, to reveal active Nucleolus Organizer Regions (NORs). Fluorescent in situ hybridization (FISH) was also applied to establish the number and location of the ribosomal gene clusters (18S and 5S rRNA genes). Counts of diploid metaphasic cells revealed a diploid modal chromosome complement composed of 48 acrocentric chromosomes in both L. analis and L. griseus. Two cytotypes were observed in L. synagris: cytotype I, with 2n=48 acrocentric chromosomes, found in 19 specimens, and cytotype II, with 46 acrocentric chromosomes and one large metacentric, found in two specimens. The large...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Ribosomal genes; NOR polymorphism; C-banding; Robertsonian rearrangement.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000100012

Astyanax aff. fasciatus Cuvier, 1819 (Teleostei; Characidae): evidences of a species complex in the upper rio Tibagi basin (Paraná, Brazil)
Provedor de dados: Neotropical Ichthyology
Autores: Artoni,Roberto Ferreira; Shibatta,Oscar Akio; Gross,Maria Claudia; Schneider,Carlos Henrique; Almeida,Mara Cristina de; Vicari,Marcelo Ricardo; Bertollo,Luiz Antonio Carlos.
Four populations of Astyanax aff. fasciatus of the upper rio Tibagi (municipal district of Ponta Grossa, Paraná State, Brazil), had their karyotypes and morphometry analyzed. The cytogenetic data show the occurrence of distinct karyotypes (cytotypes), here named cytotype A, with 2n=48 chromosomes (6m+18sm+14st+10a), cytotype B, with 2n=50 chromosomes (8m+18sm+14st+10a) and cytotype C, with 2n=50 chromosomes (8m+18sm+14st+10a). The distribution pattern of the constitutive heterochromatin was very similar between cytotypes A and B, but diverged in relation to cytotype C. Distinct cytotypes may occur in sympatry in the upper rio Tibagi region, with the exception of the Furna 2 sample, which presents cytotype A exclusively. In addition, a specimen with 2n=49...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tetra; Morphometrics; Karyotype; Sympatry; Cryptic species.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252006000200005

Comparative cytogenetics of Astyanax (Teleostei: Characidae) from the upper Paraguay basin
Provedor de dados: Neotropical Ichthyology
Autores: Teixeira,Thais K. S. S.; Venere,Paulo C.; Ferreira,Daniela C.; Mariotto,Sandra; Castro,Jonathan P.; Artoni,Roberto F.; Centofante,Liano.
ABSTRACT Astyanax is one of the most abundant and diverse taxa of fishes in the Neotropical region. In order to increase the amount of cytogenetic information for Astyanax as well as to exhibit data to subsidize future taxonomic studies, this work analyzed three species of Astyanax: two species are cryptic, and are here reported to live in syntopy (A. abramis and A. lacustris); the first karyotype description for A. pirapuan is also presented. Cytogenetic analyzes reveal a diploid number of 2n=50 chromosomes for three species, yet with differences in their karyotype morphology. The physical mapping of 18S rDNA showed up to thirteen sites in A. pirapuan and two in A. abramis and A. lacustris. The physical mapping of 5S rDNA has proven to be an effective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptic species; Fish; Karyotype; Species complex.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000100214

Chromosomal characterization of the bonytongue Arapaima gigas (Osteoglossiformes: Arapaimidae)
Provedor de dados: Neotropical Ichthyology
Autores: Marques,Debora Karla; Venere,Paulo Cesar; Galetti Junior,Pedro Manoel.
The mitotic chromosomes of the pirarucu Arapaima gigas inhabiting the middle Araguaia River and collected in the municipality of Araguaiana (MT, Brazil) were studied. The chromosomes were analyzed through Giemsa staining, C-banding, Ag-NOR staining and in situ hybridization using an 18S rRNA gene probe. The karyotype had 2n=56 comprising 14 biarmed and 14 uniarmed chromosome pairs in both sexes. No cytologically distinguishable sex chromosome was identified. A single NOR-bearing chromosome pair was detected by Ag-NOR staining and confirmed by 18S rDNA- FISH. Faint constitutive heterochromatin was C-banded in the centromeric region of some chromosomes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pirarucu; Karyotype; RDNA; Heterochromatin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252006000200007

Evidence of the color pattern variation in populations of Gymnotus pantanal (Gymnotiformes) from three streams in the upper Paraná River basin, Brazil
Provedor de dados: Neotropical Ichthyology
Autores: Frana,Vitor André; Graça,Weferson Júnio da; Gubiani,Éder André; Konerat,Jocicléia Thums; Margarido,Vladimir Pavan.
Color pattern is an important character in the systematics and alpha-taxonomy of electric fishes of the genus Gymnotus. This paper presents evidence of color variation in populations of G. pantanal found in the streams Jacutinga and Pinheirinho, in the upper Paraná River basin, southern Brazil. Color variations were corroborated for morphological and cytogenetic data. Our results show the importance of integrating morphologic and cytogenetic data in the taxonomy of the Gymnotus species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gymnotidae; Gymnotus pantherinus species-group; La Plata River basin; Karyotype.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000200011

Surprising genomic diversity in the Neotropical fish Synbranchus marmoratus (Teleostei: Synbranchidae): how many species?
Provedor de dados: Neotropical Ichthyology
Autores: Torres,Rodrigo Augusto; Roper,James Joseph; Foresti,Fausto; Oliveira,Claudio.
Chromosomes and nuclear DNA content variation in the eel Synbranchus marmoratus were studied in seven samples taken from rio Paraguay and rio Paraná basins to examine whether karyotype and nuclear DNA content variations could offer useful information for the characterization of independent evolutionary lineages in the genus and construction of evolutionary and biogeographical hypotheses. The occurrence of different karyotypes was already described for this species; however, a new cytotype from rio Miranda was discovered. Nuclear DNA content showed a wide variation among samples and individuals, ranging from 5.2 to 9.1 pg of DNA/nuclei. An analysis of variance confirmed the occurrence of significant differences among samples. In a deeper series of analyses,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish DNA; Chromosomes; Genome diversity; Karyotype; Synbranchus marmoratus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252005000200005

First chromosome data on Steindachneridion doceanum (Siluriformes: Pimelodidae): a critically endangered catfish endemic of the Doce River basin, Brazil
Provedor de dados: Neotropical Ichthyology
Autores: Swarça,Ana Claudia; Orsi,Mário Luis; Takagui,Fábio Hiroshi; Dias,Ana Lucia; Dergam,Jorge Abdala; Fenocchio,Alberto Sérgio.
ABSTRACT The present report represents the first cytogenetic description of Steindachneridion doceanum, great catfish which is currently at high extinction risk and it is listed as threatened on the red list of the Brazilian Ministry of the Environment, also are suggested karyotype relationships with other species of the same genus endemic from other river basins. The results revealed a diploid number of 2n = 56 and the karyotype composed of 18 metacentric, 20 submetacentric, 10 subtelocentric and 8 acrocentric chromosomes (NF = 104). The AgNORs and CMA3 signals were coincident in location occupying the short arm of an acrocentric chromosome pair (25th), in a secondary constriction. The 5S rDNA genes were localized on the short arms of one subtelocentric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AgNORS; C band; Karyotype; South American Catfish; Threatened species.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000400203

A case of intersex occurrence in Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae) under captivity condition: a cytogenetic and morphological study
Provedor de dados: Neotropical Ichthyology
Autores: Honji,Renato M.; Caneppele,Danilo; Pandolfi,Matias; Nostro,Fabiana L. Lo; Moreira,Renata G.
ABSTRACT Little is known about reproductive biology of endangered Steindachneridion parahybae , a gonochoristic teleost species inhabiting the Paraíba do Sul River Basin, and herein is the first description of intersex in S. parahybae juvenile. The normal appearance of ovaries and testes in juvenile from the same lot of breeding were also described for comparison, even as cytogenetic analysis was performed in these juveniles. One specimen was a priori classified as female due to the macroscopic characteristic of ovaries, with small yellow oocytes, without fringes (a main characteristic of catfish male), and larger than testes; however the microscopic analysis revealed the presence of ovotestes, including the complete spermatogenesis. S. parahybae had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histology; Karyotype; Ovary; Ovotestes; Testicles.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000400206

Cytotaxonomic diagnosis of Trichomycterus diabolus (Teleostei: Trichomycteridae) with comments about its evolutionary relationships with co-generic species
Provedor de dados: Neotropical Ichthyology
Autores: Torres,Rodrigo A.; Oliveira,Claudio; Foresti,Fausto.
The karyotype and the Ag-NOR location of a sample of Trichomycterus diabolus collected in the córrego Hortelã (Botucatu, São Paulo, Brazil) are described. The species exhibited 2n=56 chromosomes (42 metacentrics, 12 submetacentrics and 2 subtelocentrics) and the nucleolus organizing region located near to the centromere on the long arm of the largest metacentric pair. The presence of 2n=56 chromosomes in T. diabolus is an interesting characteristic since until now all cis-Andean species karyotyped presented 2n=54 chromosomes while almost all trans-Andean species presented different diploid numbers. The possible origin of this unexpected karyotypic form is discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trichomycterinae; Karyotype; Chromosome evolution; Fish cytogenetic.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252004000300003

Karyotypic characterization of Prochilodus mariae, Semaprochilodus kneri and S. laticeps(Teleostei: Prochilodontidae) from Caicara del Orinoco, Venezuela
Provedor de dados: Neotropical Ichthyology
Autores: Oliveira,Claudio; Nirchio,Mauro; Granado,Ángel; Levy,Sara.
Fish of the family Prochilodontidae are considered one of the most important components of commercial and subsistence fishery in freshwater environments in South America. This family consists of 21 species and three genera. In the present study, the karyotypes of Prochilodus mariae, Semaprochilodus kneri, and S. laticeps from Caicara del Orinoco, Bolivar State, Venezuela were studied. The species P. mariae, S. kneri and S. laticeps exhibited 2n=54 chromosomes (40 metacentric and 14 submetacentric), a single chromosome pair with nucleolus organizer regions, and a large amount of heterochromatin found at centromeric and pericentromeric positions in almost all chromosomes. The P. mariae specimens studied displayed 0 to 3 supernumerary microchromosomes. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; C-band; NOR banding; Chromossome evolution; Characiformes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252003000100005

Cytogenetical and morphological features reveal significant differences among Venezuelan and Brazilian samples of Mugil curema (Teleostei: Mugilidae)
Provedor de dados: Neotropical Ichthyology
Autores: Nirchio,Mauro; Cipriano,Roger; Cestari,Margarete; Fenocchio,Alberto.
Karyotype of M. curema from the Gulf of Mexico and Brazil have been reported as possessing chromosome complement with 2n=28 and FN=48, whereas specimens from Venezuela has been reported as possessing a diploid number 2n=24 and a conserved FN (48). Although at first sight this variation suggests the presence of a chromosomal intraspecific (interpopulational) variability, the possibility that we are dealing with two different species was examined. This work revisit the karyotypes of M. curema from Venezuela and Brazil, including new data on C-banding, and NOR localization, and compares morphologic characteristics of samples from both localities. Thus, besides diploid number, the constitutive heterochromatin distribution and NORs location, mark other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; C-band; NOR-banding; Meristic and morphometric features; Species identification.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252005000100006

Cytogenetics of Gymnogeophagus setequedas (Cichlidae: Geophaginae), with comments on its geographical distribution
Provedor de dados: Neotropical Ichthyology
Autores: Paiz,Leonardo M.; Baumgärtner,Lucas; Graça,Weferson J. da; Margarido,Vladimir P.; Pavanelli,Carla S.
ABSTRACT We provide cytogenetic data for the threatened species Gymnogeophagus setequedas, and the first record of that species collected in the Iguaçu River, within the Iguaçu National Park’s area of environmental preservation, which is an unexpected occurrence for that species. We verified a diploid number of 2n = 48 chromosomes (4sm + 24st + 20a) and the presence of heterochromatin in centromeric and pericentromeric regions, which are conserved characters in the Geophagini. The multiple nucleolar organizer regions observed in G. setequedas are considered to be apomorphic characters in the Geophagini, whereas the simple 5S rDNA cistrons located interstitially on the long arm of subtelocentric chromosomes represent a plesiomorphic character. Because G....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomes; Conservation; Iguaçu River; Karyotype; Paraná River.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000200211

Karyotype and NOR-banding of mitotic chromosomes of some Vitis L. species
Provedor de dados: Revista Brasileira de Fruticultura
Autores: Pierozzi,Neiva Izabel.
Chromosome studies were performed in V. champinii, V. cinerea, V. girdiana, V. labrusca, V. rotundifolia, V. rupestris and V. vinifera with the purpose of species characterization using chromosome morphometric data and NOR banding. A median ideogram was obtained for each species. The karyotype formula obtained varied from 7m + 12sm to 9m + 11sm. The species showed moderate chromosome asymmetry values according to TF% form, Stebbins, Romero Zarco and Paszko indices. V. champinii and V. girdiana were apart from the other species by CVcl and CVci graphic representation and also formed a group apart in the dendrogram based on Euclidian distances. The chromosome pair number 3 harbors the secondary constriction and a satellite segment in all species analyzed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; NOR-banding; Mitotic chromosomes; Vitis; Vitaceae.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000500077

A new species of Cerradomys (Mammalia: Rodentia: Cricetidae) from Central Brazil, with remarks on the taxonomy of the genus
Provedor de dados: Revista Brasileira de Zoologia
Autores: Bonvicino,Cibele R.; Casado,Flávia; Weksler,Marcelo.
Cerradomys is a Neotropical genus of cricetid rodents with seven recognized species, Cerradomys subflavus, C. maracajuensis, C. marinhus, C. scotti, C. langguthi, C. vivoi, and C. goytaca. Species of the genus are distributed throughout the open vegetation belt across South America, from northeastern and southeastern Atlantic coast of Brazil to eastern Paraguay and Western Bolivia. Here we describe a new species of Cerradomys from the state of Tocantins in Central Brazil, based on morphological, karyological and mitochondrial DNA analyses. This species is characterized by a medium body size and long tail, dense dorsal pelage, overall dorsal color gray olive lined with yellow, color of head and dorsum continuous, ventral body color slightly yellowish, skull...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity; Cerrado; Karyotype; Phylogeny; Sigmodontinae.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702014000600002

A case of leucism in the burrowing owl Athene cunicularia (Aves: Strigiformes) with confirmation of species identity using cytogenetic analysis
Provedor de dados: Revista Brasileira de Zoologia
Autores: Nogueira,Denise M; Alves,Maria Alice S.
Leucism is an inherited disorder, characterized by the lack of pigments in part or all of the body, normal coloration of the eyes and, in birds, in naked parts such as the bill and legs. This kind of disorder is sometimes erroneously designated as albinism or partial albinism. In this study, we present a case of leucism in a wild owl. The studied individual presented completely white plumage, light-yellow coloration of legs and bill and normal coloration of eyes. According to morphological features, this owl is a specimen of burrowing owl, Athene cunicularia (Molina, 1782). To confirm the species identity, we used cytogenetic analyses for karyotypic determination, comparing it to the previously described one in the literature. We also studied a captive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aberrant plumage; Bird; Chromosomes; Karyotype.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702011000100008

Morphological characterization under different ecological habitats and physical mapping of 5S and 45S rDNA in Lilium distichum with fluorescence in situ hybridization
Provedor de dados: Revista Chilena de Historia Natural
Autores: Hwang,Yoon-Jung; Song,Chang-Min; Younis,Adnan; Kim,Chang-Kil; Kang,Yoon-Im; Lim,Ki-Byung.
BACKGROUND: This study was performed to investigate the phenotypic, karyomorphological, and habitat environment characteristics of Lilium distichum that grows naturally in South Korea. Currently, this species follows limited distribution areas and its natural populations are at the brink of extinction mainly due to fragmentation or destruction its natural habitat. RESULTS: This species was distributed between approximately 1,000 and 1,500 m above sea level with an average temperature of 22°C. The soil characteristics surrounding the natural habitats included loamy and silt loam soils having organic matter content (10.82%), pH (5.22), electrical conductivity (EC) (0.37 dS/m), total nitrogen (0.45%), and cation exchange capacity (34.3 cmol+/kg). The peak...
Tipo: Journal article
Palavras-chave: Chromosome; Karyotype; Habitat; Korea; 5S rDNA; 45S rDNA.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2015000100008

A new species of Calomys Waterhouse (Rodentia, Sigmodontinae)from the Cerrado of Central Brazil
Provedor de dados: Zoologia
Autores: Bonvicino,Cibele R.; Lima,José F. S.; Almeida,Francisca C.
A new Brazilian Calomys Waterhouse, 1837 species is described based on morphologic and karyologic data. This species is endemic to the Cerrado of Central Brazil and allopatric with all other species of the genus Calomys. Its chromosome complement (2n = 46, AN = 66) is different from those described in other Calomys species. Morphometric analysis significantly distinguished this new species from other Calomys of the Brazilian fauna like C. callosus (Renger, 1830), C. expulsus (Lund, 1841) and C. tener (Winge, 1887) and placed it among the large-sized Calomys.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian Cerrado; Calomys; Karyotype; Morphology; New species.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752003000200021

Updating the list of chromosome numbers for Philodendron (Araceae)
Provedor de dados: Acta Botanica Brasilica
Autores: Vasconcelos,Emanuelle V.; Brasileiro-Vidal,Ana C.; Benko-Iseppon,Ana M.; Vasconcelos,Santelmo.
ABSTRACT Aiming for a better understanding of karyotype evolution within Philodendron, we report chromosome counts for 23 species of the genus, of which 19 are being reported for the first time, thus increasing to 84 ( ca. 17 % of the genus) the total number of species with available chromosome counts. The diploid numbers 2 n = 32 and 2 n = 34 were the most common, with 10 and 11 species, respectively, whereas only two species presented different chromosome numbers ( P. giganteum with 2 n = 30 and P. adamantinum with 2 n = 36). The results are discussed in the context of previous analyses of karyotypes of Philodendron spp., taking into account bidirectional dysploidy as the main mechanism of chromosome number evolution within the genus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aroids; Diploid number; Dysploidy; Karyotype; Philodendron.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000200309

Karyotype and genome size analyses in species of Helichrysum (Asteraceae)
Provedor de dados: Acta Botanica Brasilica
Autores: Azizi,Narjes; Sheidai,Masoud; Mozaffarian,Valiollah; Nourmohammadi,Zahra.
Karyotype studies were performed in 18 populations of eight Helichrysum species in Iran. Those species showed chromosome numbers of 2n = 2x = 14; 2n = 4x = 24, 28 and 32; 2n = 6x = 36; 2n = 7x = 42; 2n = 8x = 48; 2n = 9x = 54; and 2n = 10x = 60. The chromosome numbers of H. davisianum, H. globiferum, H. leucocephalum and H. oocephalum are reported here for the first time. New ploidy levels are reported for H. oligocephalum (2n = 4x = 24) and H. plicatum (2n = 4x = 32). The chromosomes were metacentric and submetacentric. An ANOVA among H. globiferum and H. leucocephalum populations showed significant differences for the coefficient of variation for chromosome size, total form percentage and the asymmetry indices, indicating that changes in the chromosome...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-value; Helichrysum; Karyotype; Polyploidy level.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000300009

Karyotypic analysis in species of the genus Dasyprocta (Rodentia: Dasyproctidae) found in Brazilian Amazon
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: RAMOS,ROSEMAR S. L.; VALE,WILLIAM G.; ASSIS,FÁTIMA L.
A total of 30 animals of the genus Dasyprocta were cytogenetically studied. They belong to the following species: D. prymnolopha (N=20), D. leporina (N=6), D. fuliginosa (N=1) and Dasyprocta sp. (N=3) (Dasyproctidae, Hystricognathi). Cell suspensions were obtained by peripheral blood culture, besides bone marrow and spleen cells, from D. prymnolopha and D. leporina. The diploid number was 64/65 for all samples. The karyotypes showed similarity, and chromosomal polymorphism was not detected by Giemsa conventional staining and G banding. The constitutive heterochromatin distribution at the pericentromeric region of all the chromosomes was similar in all species. D. prymnolopha, D. leporina and Dasyprocta sp. presented variation in the heterochromatical block...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Hystricognathi; Dasyprocta; Karyotype.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000100007

Range distribution and contributions to taxonomy of Elops smithi (ELOPIFORMES: ELOPIDAE)
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: SOUSA,RODRIGO P.C. DE; SODRÉ,DENISE; COSTA,RAUQUÍRIO M. DA; VALLINOTO,MARCELO; OLIVEIRA,EDIVALDO H.C.; SILVA-OLIVEIRA,GLÁUCIA C.; SAMPAIO,IRACILDA; GUIMARÃES-COSTA,AURYCÉIA.
Abstract: This study presents the first record of Elops smithi for northern Brazil. The evidence suggests this species is being misidentified incorrectly as Elops saurus in estuaries of the Western Atlantic Ocean. Here, morphological, molecular, and cytogenetic ﻿evidence identified all ladyfish specimens from one estuary in the region as E. smithi. ﻿Thus, at least Elops smithi occurs in the northern coast of Brazil and it is recommended that specimens from this region identified as E. saurus be further investigated with genetic and cytogenetic tools in order to assure a correct species identification.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elopidae; Karyotype; Ladyfish; Malacho; Mitochondrial DNA.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700622

Cytogenetics and Molecular Genetic Analysis of Chimerism in Marmosets (Callithrix: Primates)
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: SILVA,MONIQUE O.M.; ARMADA,JORGE LUÍS A. DE; VERONA,CARLOS EDUARDO S.; HELIODORO,GABRIELA; NOGUEIRA,DENISE M.
ABSTRACT The birth of fraternal twins is a characteristic frequently observed in callitrichids. Cytogenetic studies have demonstrated hematopoietic chimerism in marmosets with the occurrence of two cell lines 2n=46,XX/46,XY in females and males co-twins, without phenotypic changes. Amplification by PCR have also been used to verify the presence of the SRY gene in female chimaeras. Our aim was to verify the occurrence of chimerism in Callithrix sp. individuals considered as hybrids according to their intermediate phenotypes between C. jacchus and C. penicillata. Blood samples from 37 Callithrix sp. individuals were collected. Hematopoietic chimerism 2n=46,XX/46,XY was detected by cytogenetic analysis in five individuals, three males and two females. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; SRY; Chimaeras; Hybridization.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602793

Karyological and electrophoretic differences between Pomacea flagellata and P. patula catemacensis: Caenogastropoda: Ampullariidae
Provedor de dados: Biocell
Autores: Diupotex-Chong,María Esther; Cazzaniga,Néstor J; Uribe-Alcocer,Manuel.
The widespread Mexican apple snail Pomacea flagellata (Say 1827) and the strictly endemic "tegogolo" P. patula catemacensis (Baker 1922) (restricted to Lake Catemaco), are the only known American Ampullariidae that have haploid complements n=13. Pomacea patula catemacensis has suffered a critical reduction in abundance due to immoderate fishing for human consumption. Chromosome slides were obtained from colchicine-injected Pomacea snails collected from nine locations along the coastal zone of the Gulf of Mexico, including Lake Catemaco, for use in principal component analysis (PCA). Total proteins in foot homogenates were analyzed through isoelectric focusing (IEF) and native-PAGE electrophoresis on polyacrylamide gels. The chromosome number 2n=26 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apple snails; Endangered species; Isoelectric focusing; Karyotype; Mexico; Native-PAGE electrophoresis.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300002

Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii
Provedor de dados: Biological Research
Autores: LIU,BO; LIWANG,QI; RUIYANG,CHEN; SONG,WENQIN.
The chromosomes (2n = 2x = 24) of Larix principis-rupprechtii are composed of six pairs of large metacentrics and six pairs of medium-sized submetacentrics. The identification of homologous pairs is hampered by their high degree of similarity at the morphological level in each group. As one of the most extensively used methods in molecular cytogenetics producing chromosome landmarks, fluorescence in situ hybridization (FISH) has significantly facilitated karyotype construction, especially in species with morphologically similar chromosomes. This study developed a simple but effective use of combinatorial labeling probes to distinguish chromosomes of Larix principis-rupprechtii by multicolor FISH. Three highly repetitive sequences in Larix were selected:...
Tipo: Journal article
Palavras-chave: Combinatorial labeling probes; Fluorescence in situ hybridization; Karyotype; Larix principis-rupprechtii; LPD repeats; 25S and 5S rDNA.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100003

KARYOLOGY OF MUGIL LIZA AND M. CUREMA FROM VENEZUELA
Provedor de dados: Boletín de Investigaciones Marinas y Costeras (INVEMAR)
Autores: Nirchio T.,Mauro; Cequea,Hernán.
Karyotypes of Mugil liza and M. curema from Venezuela were studied. M. liza karyotype is 2n=48 acrocentric chromosomes, but M. curema is 2n=24, containing one submetacentric and 11 metacentric pairs. The chromosomic complement described for M. liza constitutes the first report for the species and coincides with the modal karyotype (2n=48) in the Mugilidae family. Venezuelan M. curema karyotype differs in number and shape from M. curema from Louisiana (USA), which has been reported to have a complement 2n=28 (10 metacentric pairs, two subtelocentric pairs and two acrocentric pairs).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Chromosomes.
Ano: 1998
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97611998000100004

Karyotype analysis of Minuartia mesogitana subsp. mesogitana and Minuartia elmalia (Caryophyllaceae, Alsinoideae)
Provedor de dados: Boletín de la Sociedad Argentina de Botánica
Autores: Yüce,Oguz; Eroglu,Halil Erhan; Koç,Murat; Hamzaoglu,Ergin.
Mitotic metaphase chromosomes, karyotypic characters, monoploid karyograms and ideograms of Minuartia mesogitana subsp. mesogitana and Minuartia elmalia were investigated. Analysis of somatic metaphases showed that the chromosome numbers and the karyotype formula of these taxa were 2n = 2x = 24 = 8m + 8sm + 8st for Minuartia mesogitana subsp. mesogitana and 2n = 2x = 32 = 12m + 16sm + 4st for Minuartia elmalia. No satellites were observed in the karyotypes of the taxa. The intrachromosomal and interchromosomal karyotype asymmetries were estimated with Mean Centromeric Asymmetry (MCA) and Coefficient of Variation of Chromosome Length (CVCL).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Minuartia; Karyotype; Ideogram; Karyotype asymmetry.
Ano: 2016
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722016000300006

Karyotypic studies and morphological analysis of some reproductive features in five species of Conyza (Astereae: Asteraceae) from Northeastern Argentina
Provedor de dados: Boletín de la Sociedad Argentina de Botánica
Autores: Urdampilleta,Juan D.; Amat,Aníbal G.; Bidau,Claudio J.
The cosmopolitan genus Conyza Less. comprises about 100 species, 22 of which occur in Argentina. Current taxonomic treatments, largely based on exomorphological characters, are insufficient to characterize and circumscribe some of their polymorphic species. Interspecific variations in inflorescences typology and capitula structure, as well as karyotypic aspects, were studied in five species of Conyza that naturally occur in Misiones Province (Argentina): C. blakei (Cabrera) Cabrera, C. bonariensis (L.) Cronquist var. bonariensis, C. glandulitecta Cabrera, C. primulaefolia (Lam.) Cuatrec. &amp;amp; Lourteig, C. sumatrensis (Retz.) E. Walker var. sumatrensis and C. sumatrensis (Retz.) E. Walker var. floribunda (Kunth) J. B. Marshall. Chromosome numbers for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asteraceae; Astereae; Chromosomes; Conyza; Inflorescence; Interspecific variation; Karyotype.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722005000100010

Cytomolecular characterization of cultivars and landraces of wheat tolerant and sensitive to aluminum toxicity
Provedor de dados: Bragantia
Autores: Oliveira,Érica Cristina de; Pinto-Maglio,Cecília Alzira Ferreira.
ABSTRACT Karyotyping and chromosome complement knowledge are a prerequisite for cytogenetic mapping. The aim of this study was to characterize 1 common wheat cultivar (BH 1146) and 4 durum wheat genotypes (IAC 1003, Langdon, P19 and P33) from the breeding program of the Agronomic Institute of Campinas (IAC), using karyotype analysis together with Chromomycin A3/4’-6-Diamidino-2-phenylindole (CMA/DAPI) banding techniques for longitudinal characterization of chromosomes and fluorescence in situ hybridization (FISH) in the mapping of the 45S rDNA region. Durum wheat chromosome complement number was 2n = 4x = 28 with the following karyotype formula: 22m + 6sm. Common wheat chromosome complement number was 2n = 6x = 42 with the following karyotype formula: 34m...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Triticum turgidum ssp. durum; Karyotype; CMA/DAPI banding; FISH-rDNA; Cytomolecular mapping.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000400456

Karyotipic asymmetry of both wild and cultivated species of Pennisetum
Provedor de dados: Bragantia
Autores: Techio,Vânia Helena; Davide,Lisete Chamma; Cagliari,Alexandro; Barbosa,Sandro; Pereira,Antônio Vander.
This study aimed the establishment of the relation between karyotipic asymmetry values obtained for different accessions of both wild and cultivated species of Pennisetum from Germplasm Bank of Embrapa Gado de Leite/Juiz de Fora-Minas Gerais State, Brazil. Conventional cell cycle synchronization protocols and Feulgen staining method were used to obtain metaphases plates. The wild-type accessions corresponded to the species P. setosum (2n=6x=54), P. nervosum (2n=4x=36), and P. orientale (2n=4x=36), and the cultivated to P. purpureum (2n=4x=28) and P. glaucum (2n=2x=14). No significant difference was found for the total length of chromosomes (p&gt;0.05) among the species. The analysis of intra-chromosomal asymmetry (A1) and inter-chromosomal asymmetry (A2)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pennisetum purpureum; Pennisetum glaucum; Germplasm; Karyotype.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052010000200003

Chromosomal studies of five species of the marine fishes from the Paranaguá Bay and the karyotypic diversity in the marine teleostei of the Brazilian coast
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Cipriano,Roger Raupp; Fenocchio,Alberto Sérgio; Artoni,Roberto Ferreira; Molina,Wagner; Noleto,Rafael Bueno; Kantek,Daniel Luis Zanella; Cestari,Marta Margarete.
In this study, five species of marine fishes from the Paranaguá Bay in the Brazilian coast were evaluated. Eucinostomus argenteus and Diapterus rhombeus (Gerreidae) presented 48 chromosomes, all of which more acrocentric (FN = 48); Strongylura timucu and S. marina (Belonidae) also presented 48 chromosomes, but with a higher karyotypic complexity than the Gerreidae, 10M+2SM+36A (FN = 60) and 4M+44A (FN = 52), respectively. The fifth species, Mugil curema (Mugilidae), different than the others, presented only 28 chromosomes 20M+4ST+4A (FN = 48). The species presented diversity in the karyotypic macro-structure, which should be relevant for the cytotaxonomy and the evolution of this group of the vertebrate.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine fishes; Karyotype; Cytotaxonomy; Karyotypic evolution.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200010

Cytogenetic studies in some species of Passiflora L. (Passifloraceae): a review emphasizing Brazilian species
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Souza,Margarete Magalhães; Pereira,Telma N. Santana; Vieira,Maria Lúcia Carneiro.
The Passifloraceae is represented by species of tropical and subtropical origin. The Passiflora is the richest genus with approximately 450 species, 200 of them being native to Brazil. Recent karyological studies have reported the basic chromosome number for the Passiflora genus as x = 6, whereas x = 9, x = 10 and x = 12 were established as secondary basic numbers. High rates of fertility occur in most Passiflora species, since both meiotic index and pollen viability are above 90%. Unusual meiotic behavior has been described in some taxa. Unviable pollen were observed in some diploids species. The genome size varies from 1.83 to 5.36 pg, and significant interspecific variance has been observed. Studies using the FISH methodology have shown that there are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Banding; Meiotic behavior; Pollen viability; Nuclear DNA content; FISH.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200003

Unexpected chromosomal alterations in Tayassu tajacu (Artiodactyla: Tayassuidae) in captivity
Provedor de dados: Brazilian Jounarl of Veterinary
Autores: Lima,José Fernando de Sousa; Guedes,Fátima Becker; Silva,Roxane Wirschum; Hass,Iris; Cavalli,Iglenir João; Silva,Juliana da; Freitas,Thales Renato de; Sbalqueiro,Ives José.
Wild animals have been used as bioindicators in situations in which the environment was exposed to chemical agents. In general, chemical agents may induce chromosomal aberrations, such as breaks and gaps. The peccary, Tayassu tajacu is a pig relative that exhibits a very stable karyotype with the only described alterations being of the form of the X chromosome. Chromosomal gaps and breaks were observed at high frequencies during cytogenetics analyses. These alterations were observed in the chromosomes autossomics. Reviews of the literature and of the data described herein suggests that an vermifuge, the ivermectin base, was the most likely cause of these chromosomal alterations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tayassu; Karyotype; Chromosome; Chromosomal alterations.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000100002

Karyotype of a termitophilic species of Diplopoda (Polydesmida, Chelodesmidae)
Provedor de dados: Brazilian Journal of Genetics
Autores: Fontanetti,Carmem Silvia.
This report describes the karyotype of a Brazilian Diplopod species (Arthropoda), Sandalodesmus gasparae (Schubart, Acta Zool. Lilloana Inst. "Miguel Lillo" 2: 321-440,1944), collected from a termite mound Cornitermes bequaerti. This species has 2n = 12. The sex pair could not be distinguished.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Termitophilic; Diplopoda.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400010

Karyotype of cryopreserved bone marrow cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Chauffaille,M.L.L.F.; Pinheiro,R.F.; Stefano,J.T.; Kerbauy,J.
The analysis of chromosomal abnormalities is important for the study of hematological neoplastic disorders since it facilitates classification of the disease. The ability to perform chromosome analysis of cryopreserved malignant marrow or peripheral blast cells is important for retrospective studies. In the present study, we compared the karyotype of fresh bone marrow cells (20 metaphases) to that of cells stored with a simplified cryopreservation method, evaluated the effect of the use of granulocyte-macrophage colony-stimulating factor (GM-CSF) as an in vitro mitotic index stimulator, and compared the cell viability and chromosome morphology of fresh and cryopreserved cells whenever possible (sufficient metaphases for analysis). Twenty-five bone marrow...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cryopreservation; Karyotype; Bone marrow cells.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700004

Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Santos,M.F.M.; Oliveira,F.C.A.C.; Kishimoto,R.K.; Borri,D.; Santos,F.P.S.; Campregher,P.V.; Silveira,P.A.A.; Hamerschlak,N.; Mangueira,C.L.P.; Duarte,F.B.; Crepaldi,A.H.; Salvino,M.A.; Velloso,E.D.R.P.
Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Karyotype; Leukemia; Myelodysplasia; Myeloproliferative disease.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200608

Somatic cytogenetic and azoospermia factor gene microdeletion studies in infertile men
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pina-Neto,J.M.; Carrara,R.C.V.; Bisinella,R.; Mazzucatto,L.F.; Martins,M.D.; Sartoratto,E.; Yamasaki,R.
The objective of the present study was to determine the frequency of somatic chromosomal anomalies and Y chromosomal microdeletions (azoospermia factor genes, AZF) in infertile males who seek assisted reproduction. These studies are very important because the assisted reproduction techniques (mainly intracytoplasmic sperm injection) bypass the natural selection process and some classical chromosomal abnormalities, microdeletions of AZF genes or some deleterious genic mutations could pass through generations. These genetic abnormalities can cause in the offspring of these patients male infertility, ambiguous external genitalia, mental retardation, and other birth defects. We studied 165 infertile men whose infertility was attributable to testicular problems...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Y microdeletions; Male infertility; Karyotype; Chromosome aberrations.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400017

Acute promyelocytic leukemia: the study of t(15;17) translocation by fluorescent in situ hybridization, reverse transcriptase-polymerase chain reaction and cytogenetic techniques
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Chauffaille,M.L.L.F.; Figueiredo,M.S.; Beltrani,R.; Antunes,S.V.; Yamamoto,M.; Kerbauy,J.
Acute promyelocytic leukemia (AML M3) is a well-defined subtype of leukemia with specific and peculiar characteristics. Immediate identification of t(15;17) or the PML/RARA gene rearrangement is fundamental for treatment. The objective of the present study was to compare fluorescent in situ hybridization (FISH), reverse transcriptase-polymerase chain reaction (RT-PCR) and karyotyping in 18 samples (12 at diagnosis and 6 after treatment) from 13 AML M3 patients. Bone marrow samples were submitted to karyotype G-banding, FISH and RT-PCR. At diagnosis, cytogenetics was successful in 10 of 12 samples, 8 with t(15;17) and 2 without. FISH was positive in 11/12 cases (one had no cells for analysis) and positivity varied from 25 to 93% (mean: 56%). RT-PCR was done...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute promyelocytic leukemia; Karyotype; FISH; RT-PCR; PML/RARA genem rearrangement.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600006

Cytogenetic studies of Brazilian pediatric myelodysplastic syndrome cases: challenges and difficulties in a large and emerging country
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Velloso,E.D.R.P.; Chauffaille,M.L.; Peliçario,L.M.; Tanizawa,R.S.S.; Toledo,S.R.C.; Gaiolla,R.D.; Lopes,L.F.
Myelodysplastic syndromes (MDS) and juvenile myelomonocytic leukemia (JMML) are rare hematopoietic stem cell diseases affecting children. Cytogenetics plays an important role in the diagnosis of these diseases. We report here the experience of the Cytogenetic Subcommittee of the Brazilian Cooperative Group on Pediatric Myelodysplastic Syndromes (BCG-MDS-PED). We analyzed 168 cytogenetic studies performed in 23 different cytogenetic centers; 84 of these studies were performed in patients with confirmed MDS (primary MDS, secondary MDS, JMML, and acute myeloid leukemia/MDS+Down syndrome). Clonal abnormalities were found in 36.9% of the MDS cases and cytogenetic studies were important for the detection of constitutional diseases and for differential diagnosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Childhood myelodysplastic syndromes; Juvenile myelomonocytic leukemia; Cytogenetics; Karyotype; Diagnosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100085

Cytogenetics of coffee
Provedor de dados: Brazilian Journal of Plant Physiology
Autores: Pinto-Maglio,Cecília A. F.
The genus Coffea L. has around 100 native species distributed in tropical and subtropical areas in Africa, and the most important economic species are C. arabica and C. canephora. C. arabica is exceptional in the genus since it is the only species so far analyzed which is self-compatible, and a natural polyploid with 2n=4x=44 chromosomes; it is considered to be a segmental allopolyploid because it presents a disomic inheritance and a regular meiotic behavior. All other species in the genus are self-incompatible diploids with 2n=2x=22. Cytogenetic studies in Coffea, undertaken since 1912, have followed various phases: initial studies were limited only to establishing chromosome counts. Subsequent studies characterized the karyotypes of various species using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea; Chromosomal characterization; Karyotype; Meiotic behavior; Chromomere pattern; Chromosome banding; Molecular cytogenetics.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100004

The Karyotype of Alstroemeria diluta Ehr. Bayer subsp. chrysantha (Alstroemeriaceae)
Provedor de dados: Chilean Journal of Agricultural Research
Autores: Baeza,Carlos M; Ruiz,Eduardo; Novoa,Patricio.
The karyotype of Alstroemeria diluta subsp. chrysantha Ehr. Bayer from Chile was examined. The species has 2n = 2x = 16 chromosomes, with 4m + 4sm + 2st-sat + 4t + 2t-sat. The reported karyotype is very asymmetrical (AsK % = 71.4 and Syi = 40.0%). This karyotype is similar to that published previously for Alstroemeria graminea Phil.
Tipo: Journal article
Palavras-chave: Karyotype; Ornamental plant; Chile.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000400018

Alstroemeria presliana Herb. (Alstroemeriaceae) in Chile from a Cytogenetic Perspective
Provedor de dados: Chilean Journal of Agricultural Research
Autores: Baeza,Carlos M; Schrader,Otto; Ruiz,Eduardo; Negritto,M.
Alstroemeria (Alstroemeriaceae) is an endemic genus of South America with two major distribution centers in the continent: Chile and Brazil. In Chile the genus is distributed from the North, near Iquique (20º13’ S, 70º09’ W) to the Chilean and Argentine Patagonia (53º10’ S, 70º54’ W). The central zone of Chile presents the highest number of species. A. presliana Herb. grows from Curicó (34º59’ S, 71º14’ W) to Cautín (38º45’ S, 72º34’ W) in Chile and Neuquén (36º50’ S, 71º05’ W), Argentina. A comparative karyotype study was made between a population of A. presliana subsp. presliana and a population of A. presliana subsp. australis Ehr. Bayer. Both populations presented asymmetric karyotypes, with 2n = 2x = 16 chromosomes, but with different chromosome...
Tipo: Journal article
Palavras-chave: Alstroemeriaceae; Karyotype; Chromosome numbers; Chile.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000400002