Consulta: subjectFacets:"Microsatellite"
Registros recuperados: 81
Data/hora: 04/06/2024 18:09:46

Variabilidad genética del mildiu (Bremia lactucae Regel) en Salamanca y San Miguel de Allende, Guanajuato, mediante RAPD e ISSR
Provedor de dados: 32
Autores: Martínez Martínez, Susana.
Bremia lactucae Regel causa una de las enfermedades de mayor importancia en la lechuga a nivel mundial. Debido a que este pseudohongo presenta dos tipos de reproducción: sexual y asexual, su diversidad genética es amplia y puede dar origen a nuevas razas. Dicha situación se desconoce en México, por lo que es importante conocer este aspecto en las zonas productoras de esta hortaliza, así como también la influencia que ejercen los factores ambientales sobre la incidencia y severidad del patógeno. Por ello, fueron determinadas la incidencia y severidad de B. lactucae en 15 variedades de lechuga, además se registraron variables ambientales como la temperatura, humedad relativa, humedad de la hoja y precipitación en los municipios de Salamanca y San Miguel de...
Tipo: Tesis
Palavras-chave: Bremia lactucae; Mildiu; Diversidad genética; Microsatélites; Maestría; Fitopatología; Mildew; Genetic diversity; Microsatellite.
Ano: 2008
URL: http://hdl.handle.net/10521/1675

A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages
Provedor de dados: 69
Autores: Kundu,Avijit; Sarkar,Debabrata; Bhattacharjee,Amit; Topdar,Niladri; Sinha,Mohit Kumar; Mahapatra,Bikash Sinha.
A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-NaCl DNA extraction followed by ethanol-based removal of remnant mucilages from the DNA pellet, this simple miniprep consistently and reproducibly recovers high amounts of DNA with good spectral qualities at A260/A280 and A260/A230. The purified DNA is efficiently digested by restriction endonucleases, and is suitable for PCR amplification of nuclear microsatellites with expected allele sizes.
Tipo: Journal article
Palavras-chave: Corchorus; DNA extraction; Microsatellite; Mucilage; PCR; Restriction digestion.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100010

Assessment of genetic structure in Mexican charolais herds using microsatellite markers
Provedor de dados: 69
Autores: Sifuentes-Rincón,Ana María; Puentes-Montiel,Herlinda; Parra-Bracamonte,Gaspar Manuel.
Knowledge of livestock genetic diversity is an essential step to respond to commercial demands and reach production objectives in different environments and production systems. The evaluation of animal genetic diversity is achieved by using molecular markers. Microsatellites are the most used markers for studies of this type. Eleven microsatellites were used to evaluate the genetic variation from three populations of Charolais cattle located in northeast Mexico. The studied populations exhibited a high allelic variability with a mean heterozygosity of 0.5. A moderate genetic differentiation between the Charolais populations (F ST = 0.079; P &lt; 0.001) was observed. This suggests subdivisions in Charolais breed established in Mexico, due to genetic...
Tipo: Journal article
Palavras-chave: Charolais; Genetic diversity; Microsatellite.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400002

QTL affecting body weight in a candidate region of cattle chromosome 5
Provedor de dados: 74
Autores: Machado,Mariana B.B.; Alencar,Maurício M.; Pereira,Andréa P.; Oliveira,Henrique N.; Casas,Eduardo; Coutinho,Luis L.; Regitano,Luciana C.A.
The objective of this work was to identify QTLs for liveweight in a candidate region of bovine chromosome 5. Half-sib families from two lines, one traditional and the other new, of Canchim beef cattle (5/8 Charolais + 3/8 Zebu) were genotyped for four microsatellite markers, including the microsatellite in the IGF-1 (insulin-like growth factor-1) promoter region. Significant differences in allele distribution between the two lines were found for three markers. Interval mapping analyses in this region indicated the presence of a QTL controlling birth weight (p &lt; 0.05) and of a QTL influencing breeding value for yearling weight (p &lt; 0.01) in the newer line of the breed. The previously identified interaction between the IGF-1 genotype and genetic group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: QTL; Growth traits; Microsatellite; Bovine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300008

Genetic structure and conservation of Mountain Lions in the South-Brazilian Atlantic Rain Forest
Provedor de dados: 74
Autores: Castilho,Camila S.; Marins-Sá,Luiz G.; Benedet,Rodrigo C.; Freitas,Thales R.O.
The Brazilian Atlantic Rain Forest, one of the most endangered ecosystems worldwide, is also among the most important hotspots as regards biodiversity. Through intensive logging, the initial area has been reduced to around 12% of its original size. In this study we investigated the genetic variability and structure of the mountain lion, Puma concolor. Using 18 microsatellite loci we analyzed evidence of allele dropout, null alleles and stuttering, calculated the number of allele/locus, PIC, observed and expected heterozygosity, linkage disequilibrium, Hardy-Weinberg equilibrium, F IS, effective population size and genetic structure (MICROCHECKER, CERVUS, GENEPOP, FSTAT, ARLEQUIN, ONESAMP, LDNe, PCAGEN, GENECLASS software),we also determine whether there...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucaria Forest; Atlantic Rain Forest; Conservation; Genetic diversity; Microsatellite.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100009

Genetic characterization of the Neotropical catfish Pimelodus maculatus (Pimelodidae, Siluriformes) in the Upper Uruguay River
Provedor de dados: 74
Autores: Ribolli,Josiane; Melo,Cláudio Manoel Rodrigues de; Zaniboni-Filho,Evoy.
Freshwater fish present unique challenges when one attempts to understand the factors that determine the structure of their populations. Habitat fragmentation is a leading cause of population decline that threatens ecosystems worldwide. In this study, we investigated the conservation status of genetic variability in the Neotropical catfish (Pimelodus maculatus). Specifically, we examined the structure and genetic diversity of this species in a region of the Upper Uruguay River fragmented by natural barriers and dams. There was no genetic structure among the four sites analyzed, indicating the existence of only one population group. A combination of environmental management and genetic monitoring should be used to minimize the impact of impoundment on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Dams; Habitat fragmentation; Microsatellite.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500009

Comparison of the effectiveness of microsatellites and SNP panels for genetic identification, traceability and assessment of parentage in an inbred Angus herd
Provedor de dados: 74
Autores: Fernández,María E.; Goszczynski,Daniel E.; Lirón,Juan P.; Villegas-Castagnasso,Egle E.; Carino,Mónica H.; Ripoli,María V.; Rogberg-Muñoz,Andrés; Posik,Diego M.; Peral-García,Pilar; Giovambattista,Guillermo.
During the last decade, microsatellites (short tandem repeats or STRs) have been successfully used for animal genetic identification, traceability and paternity, although in recent year single nucleotide polymorphisms (SNPs) have been increasingly used for this purpose. An efficient SNP identification system requires a marker set with enough power to identify individuals and their parents. Genetic diagnostics generally include the analysis of related animals. In this work, the degree of information provided by SNPs for a consanguineous herd of cattle was compared with that provided by STRs. Thirty-six closely related Angus cattle were genotyped for 18 STRs and 116 SNPs. Cumulative SNPs exclusion power values (Q) for paternity and sample matching...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite; Single nucleotide polymorphism; Exclusion probability; Genetic identification; Bovine.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200008

Genetic characterization of Aberdeen Angus cattle using molecular markers
Provedor de dados: 74
Autores: Vasconcellos,Luciana Pimentel de Mello Klocker; Tambasco-Talhari,Daniella; Pereira,Andréa Pozzi; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida.
Aberdeen Angus beef cattle from the Brazilian herd were studied genetically using restriction fragment length polymorphism (RFLP) of the kappa-casein - HinfI (CSN3 - HinfI), beta-lactoglobulin - HaeIII (LGB - HaeIII) and growth hormone AluI (GH- AluI) genes, as well as four microsatellites (TEXAN15, CSFM50, BM1224 and BM7160). The RFLP genotypes were determined using the polymerase chain reaction (PCR) followed by digestion with restriction endonucleases and electrophoresis in agarose gels. With the exception of the microsatellite BM7160, which was analyzed in an automatic sequencer, the PCR products were genotyped by silver staining. The allele and genotype frequencies, heterozygosities and gene diversity were estimated. The values for these parameters of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aberdeen Angus; Bovine; DNA; Characterization; RFLP; Microsatellite.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200005

Genetic variability of 10 microsatellite markers in the characterization of Brazilian Nellore cattle (Bos indicus)
Provedor de dados: 74
Autores: Cervini,Marcelo; Henrique-Silva,Flávio; Mortari,Norma; Matheucci Jr,Euclides.
We assessed the polymorphism of 10 microsatellites in Brazilian Nellore cattle (Bos indicus) using a commercial multiplex system. Allele frequencies, polymorphism information content, heterozygosity and exclusion probability were calculated. Allele frequencies revealed that in the sample analyzed the markers were not equally polymorphic. The exclusion probabilities and the polymorphism information content of some loci in Nellore cattle were lower than in Bos taurus breeds. When all the microsatellites were considered the combined exclusion probability was 0.9989. This multiplex analysis can contribute toward pedigree information, adequate genetic improvements and breeding programs.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alleles; Frequencies; Microsatellite; Nellore; Polymorphism; Zebu.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300015

Genetic diversity in section Rhizomatosae of the genus Arachis (Fabaceae) based on microsatellite markers
Provedor de dados: 74
Autores: Angelici,Carla M.L.C.D.; Hoshino,Andrea Akemi; Nóbile,Paula Macedo; Palmieri,Dario Abel; Valls,José F. Montenegro; Gimenes,Marcos A.; Lopes,Catalina Romero.
The genus Arachis (Fabaceae) native to South America, contains 80 species divided into nine sections, three of which contain species of special economic importance such as the cultivated peanut (Arachis hypogaea), belonging to the section Arachis, and some perennial forage species from sections Caulorrhizae and Rhizomatosae. We used microsatellite markers to assay genetic variability among 77 accessions of four species from section Rhizomatosae, the diploid Arachis burkartii (2n = 2x = 20) and the tetraploid Arachis glabrata, Arachis pseudovillosa and Arachis nitida (2n = 4x = 40). A total of 249 alleles were found in the fifteen loci analyzed and a high degree of intra and interspecific polymorphism was detected. The lowest intraspecific variation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachis; Genetic diversity; Microsatellite; Peanut; Rhizomatosae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100016

Genetic diversity of Morato's Digger Toad, Proceratophrys moratoi: spatial structure, gene flow, effective size and the need for differential management strategies of populations
Provedor de dados: 74
Autores: Arruda,Mauricio P.; Costa,William P.; Recco-Pimentel,Shirlei M.
Abstract The Morato's Digger Toad, Proceratophrys moratoi, is a critically endangered toad species with a marked population decline in southern Brazilian Cerrado. Despite this, new populations are being discovered, primarily in the northern part of the distribution range, which raises a number of questions with regard to the conservation status of the species. The present study analyzed the genetic diversity of the species based on microsatellite markers. Our findings permitted the identification of two distinct management units. We found profound genetic structuring between the southern populations, on the left margin of the Tietê River, and all other populations. A marked reduction was observed in the contemporary gene flow among the central populations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proceratophrys moratoi; Microsatellite; Population structure; Management unit; Conservation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300502

Microsatellite studies on an isolated population of African descent in the Brazilian state of Bahia
Provedor de dados: 74
Autores: Barbosa,Ana Angélica Leal; Sousa,Sandra Mara Bispo; Abé-Sandes,Kiyoko; Alonso,Carlos Alberto; Schneider,Vicente; Costa,Denise C.C.; Cavalli,Iglenir João; Azevêdo,Eliane Elisa Souza.
The African descent population of the Bananal community in the Brazilian state of Bahia (BA) was characterized as a genetic isolate and analyzed for some short tandem repeat (STR) microsatellite autosomic polymorphic loci (CSF1PO, TH01, TPOX, F13A1, FESFPS and vWA). These genetic variants were further compared to data obtained from an urban sample from the town of Jequié (BA) regarding demographic and anthropogenetic aspects. The Bananal sample comprised 32 unrelated individuals whereas Jequié was represented by 76 individuals. The Bananal Negroid Phenotypic Index (NPI) was 0.98 and the Negroid Cultural Index (NCI) 0.24. Consanguineous marriages occurred at a frequency of 34.61% and the F value was 0.0126. All six loci studied were in Hardy-Weinberg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite; African-descendant isolate.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100004

Genetic diversity and population structure of the Guinea pig (Cavia porcellus, Rodentia, caviidae) in Colombia
Provedor de dados: 74
Autores: Burgos-Paz,William; Cerón-Muñoz,Mario; Solarte-Portilla,Carlos.
The aim was to establish the genetic diversity and population structure of three guinea pig lines, from seven production zones located in Nariño, southwest Colombia. A total of 384 individuals were genotyped with six microsatellite markers. The measurement of intrapopulation diversity revealed allelic richness ranging from 3.0 to 6.56, and observed heterozygosity (Ho) from 0.33 to 0.60, with a deficit in heterozygous individuals. Although statistically significant (p &lt; 0.05), genetic differentiation between population pairs was found to be low. Genetic distance, as well as clustering of guinea-pig lines and populations, coincided with the historical and geographical distribution of the populations. Likewise, high genetic identity between improved and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food security; Microsatellite; Population structure.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400027

Genetic variability of two populations of Pseudoplatystoma reticulatum from the Upper Paraguay River Basin
Provedor de dados: 74
Autores: Abreu,Marcia Matos de; Pereira,Luiz Henrique Garcia; Vila,Vilma Barretto; Foresti,Fausto; Oliveira,Claudio.
Catfishes of the genus Pseudoplatystoma are very important species due to both their high commercial value and their ecological role as voracious predators. They undertake lengthy migratory movements during their life-cycle, this including reproductive migration which occurs from October to December in the rainy season. In the present study, seven microsatellite loci were analyzed to access genetic variability in two samples of P. reticulatum from the Upper Paraguay Basin. The loci were highly polymorphic (mean = 7.28). According to all analysis, the two samples of P. reticulatum revealed pronounced genetic differentiation. Fst value was 0.2290, Rst value 0.1067 and AMOVA 22.90% (Fst) and 10.67% (Rst), all being highly significant (p &lt; 0.001). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Population genetics; Microsatellite; Homing; Population structure.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400032

Demarcation of informative chromosomes in tropical sweet corn inbred lines using microsatellite DNA markers
Provedor de dados: 74
Autores: Kashiani,Pedram; Saleh,Ghizan; Panandam,Jothi Malar; Abdullah,Nur Ashikin Psyquay; Selamat,Ahmad.
A study of genetic variation among 10 pairs of chromosomes extracted from 13 tropical sweet corn inbred lines, using 99 microsatellite markers, revealed a wide range of genetic diversity. Allelic richness and the number of effective alleles per chromosome ranged from 2.78 to 4.33 and 1.96 to 3.47, respectively, with respective mean values of 3.62 and 2.73. According to the Shannon's information index (I) and Nei's gene diversity coefficient (Nei), Chromosome 10 was the most informative chromosome (I = 1.311 and Nei = 0.703), while Chromosome 2 possessed the least (I = 0.762 and Nei = 0.456). Based on linkage disequilibrium (LD) measurements for loci less than 50 cM apart on the same chromosome, all loci on Chromosomes 1, 6 and 7 were in equilibrium. Even...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite; Inbred line; Chromosome; Genetic variation; Linkage disequilibrium.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400012

LEI0258 microsatellite variability and its relationship to B-F haplotypes in Brazilian (blue-egg Caipira) chickens
Provedor de dados: 74
Autores: Lima-Rosa,Carlos André da Veiga; Canal,Cláudio Wageck; Fallavena,Paulo Roberto Vargas; Freitas,Loreta Brandão de; Salzano,Francisco Mauro.
A total of 149 chickens from two different sources (one non-commercial, the other commercial) was tested for variability of the LEI0258 microsatellite. Fifty- three genotypes, explainable by 15 alleles, were found. There are clear allele and heterozygosity differences between the two samples. One of them was simultaneously studied for the MHC B-F haplotypes. Strong genetic disequilibrium was observed between the variants of the two systems, therefore providing a cheap alternative for MHC genotyping.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Microsatellite; LEI0258; B-F haplotypes; Brazilian chickens.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300008

Genetic diversity and population structure of bocachico Prochilodus magdalenae (Pisces, Prochilodontidae) in the Magdalena River basin and its tributaries, Colombia
Provedor de dados: 74
Autores: Berdugo,Gilberto Orozco; Narváez Barandica,Juan C.
Prochilodus magdalenae is an endemic freshwater fish that occurs in the Magdalena, Sinú and Atrato hydrographic basins. It has an important economic role and is a food resource for the artisanal fishing communities. Its socioeconomic importance contrasts with the current status of its fisheries, where stocks are being depleted. Considering its importance and lack of information on its genetic structure, we used seven microsatellite markers to assess the genetic structure of wild populations of P. magdalenae. The genetic diversity was assessed and the population genetic structure was estimated through Fst, analysis of molecular variance and Bayesian analysis. A total of 290 alleles were found in all loci throughout all population. The high polymorphism...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neotropical fish; Microsatellite; Conservation genetics; Population genetics.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100008

Comparative analysis of microsatellite variability in five macaw species (Psittaciformes, Psittacidae): application for conservation
Provedor de dados: 74
Autores: Presti,Flavia T.; Oliveira-Marques,Adriana R.; Caparroz,Renato; Biondo,Cibele; Miyaki,Cristina Y.
Cross-amplification was tested and variability in microsatellite primers (designed for Neotropical parrots) compared, in five macaw species, viz., three endangered blue macaws (Cyanopsitta spixii [extinct in the wild], Anodorhynchus leari [endangered] and Anodorhynchus hyacinthinus [vulnerable]), and two unthreatened red macaws (Ara chloropterus and Ara macao). Among the primers tested, 84.6% successfully amplified products in C. spixii, 83.3% in A. leari, 76.4% in A. hyacinthinus, 78.6% in A. chloropterus and 71.4% in A. macao. The mean expected heterozygosity estimated for each species, and based on loci analyzed in all the five, ranged from 0.33 (A. hyacinthinus) to 0.85 (A. macao). As expected, the results revealed lower levels of genetic variability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Cross-amplification; Macaw; Microsatellite; Psittaciformes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200030

Characterization of six microsatellite loci in  Myrica faya  (Myricaceae)   and cross amplification in the endangered endemic  M. rivas-martinezii    in Canary Islands, Spain
Provedor de dados: 74
Autores: González-Pérez,Miguel A.; Newton,Craig; Sosa,Pedro A.; Rivero,Elizabeth; González-González,Edna A.
Six novel polymorphic microsatellite markers were isolated from enriched libraries in  Myrica faya  Ait., recently renamed  Morella faya , (fayatree, firetree, or firebush) in order to examine the genetic diversity in natural populations. Also, test cross-specific amplification and genetic diversity in  Myrica rivas-martinezii,  which is endemic on the Canary islands. Microsatellite loci were screened in 225 individuals of both species from different islands of the Canarian archipelago. All markers were successfully amplified from both  Myrica  species, with an average number of 6.5 and 9.3 alleles per locus in  M. rivas-martinezii  and  M. faya , respectively. There was no evidence for linkage disequilibrium between loci, and the probability of null...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myrica rivas-martinezii; Myrica faya; Microsatellite; Canary Islands; Genetic diversity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100017

Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
Provedor de dados: 74
Autores: Lopes,Denilce Meneses; Campos,Lúcio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.
Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melipona; Stingless bees; Microsatellite; Transferability.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200030

Lack of association between genetic polymorphisms in IGF1 and IGFBP3 with twin births in a Brazilian population (Cândido Godói, Rio Grande do Sul)
Provedor de dados: 74
Autores: Oliveira-Klein,Mariana de; Cardoso-dos-Santos,Augusto César; Tagliani-Ribeiro,Alice; Fagundes,Nelson Rosa; Matte,Ursula; Schuler-Faccini,Lavinia.
Abstract Insulin-like growth factor (IGF-1) is an important peptide hormone involved in the reproduction and fetal development of mammals, and it is suggested that it may influence the human twinning rate. This study aimed to test such possible association, investigating the genetic polymorphisms IGF1 (CA)n and IGFBP3 rs2854744 in the population from Candido Godoi (CG), a small city located in the South of Brazil that has a high prevalence of twin births. A case-control study was performed comprising a total of 39 cases (representing about 40% of the mothers of twins who were born in CG after 1995) and 214 controls (mothers of non-twin children), 97 of whom were living in CG while 117 were living in Porto Alegre. DNA was extracted from blood leucocytes and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin-like growth factor; Founder effect; Microsatellite; Reproduction; Twinning.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500775

Description of novel microsatellite loci in the Neotropical fish Prochilodus argenteus and cross-amplification in P. costatus and P. lineatus
Provedor de dados: 74
Autores: Barbosa,Anna C.D.R.; Galzerani,Felipe; Corrêa,Thaís C.; Galetti Jr.,Pedro M.; Hatanaka,Terumi.
Prochilodus is one of the most important fish resources of South America, in addition to the important role it plays in nutrient cycling of Neotropical rivers. In the present study, we describe the isolation and characterization of nine novel microsatellite loci in Prochilodus argenteus. The number of alleles per polymorphic locus varied from 5 (Par76) to 21 (Par85), revealing a total of 116 alleles. The values of observed and expected heterozygosities ranged from 0.629 (Par69) to 0.926 (Par85 and Par86) and from 0.643 (Par66) to 0.931 (Par80), respectively. Furthermore, the ability of these and other previously described microsatellite markers to amplify orthologous loci was tested in two related species, Prochilodus costatus and Prochilodus lineatus....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cross-species amplification; Enrichment libraries; Microsatellite; Prochilodus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200032

Parentage testing and effect of misidentification on the estimation of breeding value in Gir cattle
Provedor de dados: 74
Autores: Baron,Erica E.; Martinez,Mário L.; Verneque,Rui S.; Coutinho,Luiz L.
A correct relationship among sires is essential for an efficient breeding program. Microsatellite markers were used in progeny tests, to assess the paternity of seventy-four probable offspring of nine Gir dairy sires. A 36% misidentification rate was observed; however, these errors had minimal effects on the ranking of the nine bulls with regard to their genetic values. The results suggest that paternity tests should be performed in breeding programs, in order to prevent inappropriate paternities from influencing the genetic value of bulls in the future.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite; Gir; Cattle; Breeding; Parentage; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400006

Structure and genetic relationships between Brazilian naturalized and exotic purebred goat domestic goat (Capra hircus) breeds based on microsatellites
Provedor de dados: 74
Autores: Oliveira,Joelliton Domingos de; Igarashi,Maria Luiza Silveira de Paiva; Machado,Théa Mírian Medeiros; Miretti,Marcos Mateo; Ferro,Jesus Aparecido; Contel,Eucleia Primo Betioli.
The genetic relationships and structure of fourteen goat (Capra hircus) populations were estimated based on genotyping data from 14 goat populations (n = 410 goats) at 13 microsatellite loci. We used analysis of molecular variance (AMOVA), principal component analysis (PCA) and F statistics (F IS, F IT and F ST) to evaluate the genetic diversity (Ho, He and ad) of the goats. Genetic distances between the 14 goat populations were calculated from allelic frequency data for the 13 microsatellite markers. Moderate differentiation was observed for the populations of the undefined breeds (including the Anglo-Nubian-M breed), the naturalized Brazilian breeds (Moxotó, Canindé), the exotic purebred breeds (Alpine, Saanen, Toggenbourg and Anglo-Nubian) and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goat; Microsatellite; Genetic distance; PCA; AMOVA; Genetic relationships; Genetic diversity; F statistics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300010

Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
Provedor de dados: 74
Autores: Jamsari,Amirul Firdaus Jamaluddin; Min-Pau,Tan; Siti-Azizah,Mohd Nor.
Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.120 to 0.880 and 0.117 to 0.698, respectively. A single locus (CS1-C07) was significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These novel markers would be useful for population genetic studies of the C. striata.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Channa striata; Population genetics; Microsatellite; Multiplex PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200029

A hybrid zone of the genus Ctenomys: a case study in southern Brazil
Provedor de dados: 74
Autores: Castilho,Camila S.; Gava,Adriana; Freitas,Thales R.O. de.
We describe variation at microsatellite loci and the chromosomal polymorphisms of a hybrid population, and hybridizing populations of Ctenomys minutus (the minor tuco-tuco) from the coastal plain of Rio Grande do Sul, southern Brazil. Cytogenetic analysis and a survey of six microsatellite loci included 101 specimens of C. minutus from the parental populations (2n/AN = 42/74 and 48a/76) and their contact zone. Cytogenetic analysis recorded 26 different karyotypes exhibited by 50 individuals from the hybrid population. Of the 26 karyotypes, only 14% presented a parental-like configuration, and none had the combinations of 2n and AN expected for an F1 hybrid. The remaining karyotypes were alternative hybrid forms, with 2n varying from 42 to 46 and AN from 68...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome polymorphism; Subterranean rodent; Karyotypes; Microsatellite.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600014

Genetic diversity analysis of Cuban traditional rice (Oryza sativa L.) varieties based on microsatellite markers
Provedor de dados: 74
Autores: Alvarez,Alba; Fuentes,Jorge Luis; Puldón,Violeta; Gómez,Pedro Julio; Mora,Leonor; Duque,Miriam C.; Gallego,Gerardo; Tohme,Joe M.
Microsatellite polymorphism was studied in a sample of 39 traditional rice (Oryza sativa L.) varieties and 11 improved varieties widely planted in Cuba. The study was aimed at assessing the extent of genetic variation in traditional and improved varieties and to establish their genetic relationship for breeding purposes. Heterozygosity was analyzed at each microsatellite loci and for each genotype using 10 microsatellite primer pairs. Between varieties genetic relationship was estimated. The number of alleles per microsatellite loci was 4 to 8, averaging 6.6 alleles per locus. Higher heterozygosity (H) was found in traditional varieties (H TV = 0.72) than in improved varieties (H IV = 0.42), and 68% of the total microsatellite alleles were found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Microsatellite; Rice; Traditional germplasm.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600014

Microsatellite analysis to estimate genetic relationships among five bulgarian sheep breeds
Provedor de dados: 74
Autores: Kusza,Szilvia; Dimov,Doytcho; Nagy,István; Bõsze,Zsuzsanna; Jávor,András; Kukovics,Sándor.
Herein, genetic relationships among five breeds of Bulgarian sheep were estimated using microsatellite markers. The total number of alleles identified was 226 at the 16 loci examined. D A distance values were used for phylogenetic tree construction with the UPGMA algorithm. The two Tsigai and two Maritza populations were found to be geneticallvery closely related to each other y (0.198, and 0.258 respectively). The Pleven Black Head population was distinct from the other four. These results could be useful for preserving genes in these breeds, thereby ensuring their preservation in Bulgaria.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic relationships; Bulgarian sheep breeds; Microsatellite.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100010

In silico characterization of microsatellites in Eucalyptus spp.: abundance, length variation and transposon associations
Provedor de dados: 74
Autores: Rabello,Edenilson; Souza,Adriane Nunes de; Saito,Daniel; Tsai,Siu Mui.
This study assessed the abundance of microsatellites, or simple sequence repeats (SSR), in 19 Eucalyptus EST libraries from FORESTs, containing cDNA sequences from five species: E. grandis, E. globulus, E. saligna, E. urophylla and E. camaldulensis. Overall, a total of 11,534 SSRs and 8,447 SSR-containing sequences (25.5% of total ESTs) were identified, with an average of 1 SSR/2.5 kb when considering all motifs and 1 SSR/3.1 kb when mononucleotides were not included. Dimeric repeats were the most abundant (41.03%), followed by trimerics (36.11%) and monomerics (19.59%). The most frequent motifs were A/T (87.24%) for monomerics, AG/CT (94.44%) for dimerics, CCG/CGG (37.87%) for trimerics, AAGG/CCTT (18.75%) for tetramerics, AGAGG/CCTCT (14.04%) for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus; EST; Microsatellite; Simple sequence repeat (SSR); Molecular marker.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400013

Isolamento e caracterização de marcadores microssatélites para Araucaria angustifólia (Araucariaceae).
Provedor de dados: 14
Autores: SCHMIDT, A. B.; CIAMPI, A. Y.; GUERRA, M. P.; NODARI, R. O. N.
bitstream/CENARGEN/29551/1/ct053.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Microsatellite; Diversidade genética.; Araucária Angustifólia; Conservação.
Ano: 2007
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/189437

High dispersal capacity of Culicoides obsoletus (Diptera: Ceratopogonidae), vector of bluetongue and Schmallenberg viruses, revealed by landscape genetic analyses
Provedor de dados: 5
Autores: Mignotte, Antoine; Garros, Claire; Dellicour, Simon; Jacquot, Maude; Gilbert, Marius; Gardès, Laetitia; Balenghien, Thomas; Duhayon, Maxime; Rakotoarivony, Ignace; De Wavrechin, Maïa; Huber, Karine.
Background In the last two decades, recurrent epizootics of bluetongue virus and Schmallenberg virus have been reported in the western Palearctic region. These viruses affect domestic cattle, sheep, goats and wild ruminants and are transmitted by native hematophagous midges of the genus Culicoides (Diptera: Ceratopogonidae). Culicoides dispersal is known to be stratified, i.e. due to a combination of dispersal processes occurring actively at short distances and passively or semi-actively at long distances, allowing individuals to jump hundreds of kilometers. Methods Here, we aim to identify the environmental factors that promote or limit gene flow of Culicoides obsoletus, an abundant and widespread vector species in Europe, using an innovative framework...
Tipo: Text
Palavras-chave: Culicoides obsoletus; Landscape genetics; Microsatellite; Dispersal; Palearctic region.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00678/79022/81428.pdf

Characterization of thirteen new polymorphic microsatellite markers from the honeycomb grouper Epinephelus merra
Provedor de dados: 5
Autores: Muths, Delphine; Bourjea, Jerome.
Epinephelus merra (Serranidae) is the most common and widespread species of this genus, mostly found in reef habitats from South Africa to Central Pacific. Thirteen polymorphic microsatellite loci were isolated and characterized from this species. Polymorphism was studied in two localities from the Comoros archipelago-Moh,li and Moroni. Allelic diversity was 7.5, polymorphism ranging from 3 to 13 alleles. Overall heterozygosity was high with an average observed heterozygosity of 0.587. Three of these loci were characterised by heterozygote deficiencies for both populations. Finally, ten of the 13 developed primers could be used in future population genetics study of E.merra that might be useful in a context of marine biodiversity conservation.
Tipo: Text
Palavras-chave: Microsatellite; Epinephelus merra; Reef fish; Indian ocean.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00044/15529/12957.pdf

Genetic structure of the reef grouper Epinephelus merra in the West Indian Ocean appears congruent with biogeographic and oceanographic boundaries
Provedor de dados: 5
Autores: Muths, Delphine; Tessier, Emmanuel; Bourjea, Jerome.
The reef fauna connectivity of the West Indian Ocean (WIO) is one of the least studied globally. Here we use genetic analyses of the grouper Epinephelus merra (Bloch 1793) to determine patterns of connectivity and to identify barriers to dispersal in this WIO marine area. Phylogeographic and population-level analyses were conducted on cytochrome b sequences and microsatellites (13 loci) from 557 individuals sampled in 15 localities distributed across the West Indian Ocean. Additional samples from the Pacific Ocean were used to benchmark the WIO population structure. The high level of divergence revealed between Indian and Pacific localities (of about 4.5% in sequences) might be the signature of the major tectonic and climatic changes operating at the...
Tipo: Text
Palavras-chave: Cytochrome b; Marine connectivity; Microsatellite; Reef fish; West Indian Ocean.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00189/30046/28566.pdf

Characterisation of 22 polymorphic microsatellite loci for the Broadbill Swordfish, Xiphias gladius
Provedor de dados: 5
Autores: Bradman, H.; Grewe, P.; Bourjea, Jerome; Muths, Delphine; Appleton, B.
The Broadbill Swordfish is harvested by fisheries throughout the world. In order to devise more effective management strategies, we need a clear understanding of the population structure of the species. From a library of 29 tetranucleotide repeats, 22 microsatellite markers were characterised for 94 swordfish samples captured from eastern and western Australia. The number of alleles ranged from 2 to 26 and observed heterozygosity from 0.066 to 0.923. We have identified 18 microsatellites that will be valuable in the examination of swordfish population structure.
Tipo: Text
Palavras-chave: Swordfish; Xiphias gladius; Microsatellite; Genetic markers.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00037/14805/12428.pdf

Utilisation d'un marqueur microsatellite pour l'étude des contributions parentales chez l'huître creuse Crassostrea gigas
Provedor de dados: 5
Autores: Hervouet, Veronique.
Les marqueurs génétiques très polymorphes sont des outils particulièrement intéressants pour l'analyse de contributions parentales. Dans notre étude, nous avons utilisé un marqueur microsatellite pour analyser les contributions parentales dans deux croisements contrôlés chez l'huître creuse Crassostrea gigas. 5 mâles et 5 femelles, hétérozygotes à ce locus, et portant chacun des allèles différents (soit 20 allèles ségrégés au total) ont été croisés de deux manières différents: soit en mélangeant toutes les gamètes mâles d'une part, toutes les gamètes femelles d'autre part, et en réalisant un croisement entre ces deux mélanges; soit en fécondant séparément chacune des 5 femelles par chacun de 5 mâles, soit 25 croisement séparés. Pour le deuxième type de...
Tipo: Text
Palavras-chave: Contributions parentales; Compétition; Microsatellite; Crassostrea gigas.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00032/14372/11661.pdf

Hybridation naturelle entre les deux sous-espèces d'huîtres creuses Crassostrea gigas et Crassostrea angulata au sud de l'Europe
Provedor de dados: 5
Autores: Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie; Boudry, Pierre.
Crassostrea angulata and Crassostrea gigas, two cupped oysters of Asian origin (O'Foighil et al, 1998), have been introduced successively in Europe during the XVlth and the XXth century (Grizel et Héral, 1991). The present populations of C. angulata appear to be limited to the south of Spain and Portugal, whereas the distribution of C. gigas in Europe is rising because of the development of oyster farming. The distinction between the two subspecies in based on a RFLP marker of the mitochondrial genome (COI) which shows a strong genetic differentiation between the two subspecies populations (Boudry et al., 1998). On the contrary, the nuclear markers (microsatellites) showed a smaller genetic differentiation between them (Huvet et al, under press). The...
Tipo: Text
Palavras-chave: Microsatellite; Hybridation; Crassostrea angulata; Crassostrea gigas; Huîtres; Génétique.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3282.pdf

Mise en évidence expérimentale de la variance du succès reproducteur chez l'huître creuse Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Kotoulas, G.; Bonhomme, Francois.
Oysters, like many other marine species, are characterized by very high fertilities. Natural or hatchery populations, often present very low Ne/N ratios (Hedgecock, 1994). It can be explained by very high variances of their breeding success (Li and Hedgecock, 1998), the effective population size being estimated through the temporal variance of the allelic frequencies in natural populations (Hedgecock et al., 1992) or, more directly, through the estimated number of founders in hatchery populations (Launey, 1998). This document is a presentation of the results of a number of experiments, realised in the scope of the European program "GENEPHYS" (FAIR 95-421), and using microsatellite markers to analyse the parental contributions in the flat oyster Crassostrea...
Tipo: Text
Palavras-chave: Microsatellite; Succès reproducteur; Contributions parentales; Génétique; Crassostrea gigas; Huîtres.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/acte-3454.pdf

Développement de marqueurs moléculaires liés à la résistance à la mortalité estivale chez l'huître creuse Crassostrea gigas - Approche QTL
Provedor de dados: 5
Autores: Sauvage, Christopher.
The Pacific oyster Crassostrea gigas is the most cultivated marine species in the world (4.2 million tonnes for a turnover of 3.5 billion US dollars according to the FAO 2005). Flat oyster culture is one of the components of the French economic activity. However, for more than 15 years, farms have had to face some summer mortality episodes (30 %-60 %), that could jeopardize European mollusc culture competitivity. More than the economical importance of C. gigas, which justifies a great research effort, oysters constitute a study model of the complex physiological and genetic basis (i.e. growth, reproduction, and survival) strongly correlated to the oyster's response to different environmental conditions. One of the considered solutions is the selection of...
Tipo: Text
Palavras-chave: Crassostrea gigas; Huître; Microsatellite; SNP (Single Nucleotide Polymorphism); QTL (Quantitative Trait Locus); Phénotype; Génotype; Cartographie; Marqueurs moléculaires; Sélection Génétique; Génétique.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/these-4544.pdf

Evidence for natural hybrtidisation between the two oyster sub-species Crassostrea gigas and Crassostrea angulata in Southern Europe
Provedor de dados: 5
Autores: Boudry, Pierre; Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie.
Crassostrea angulata and Crassostrea gigas, two cupped oyster taxa of Asian origin (O'Foighil et al, 1998), were successively introduced into Europe  during the XVIth  and the XXth century respectively (Héral and Grizel, 1991). To date, populations presumed to be C. angulata remain only in the south of Spain and Portugal, while the geographic range of C. gigas is expending, notably due to aquaculture. The two taxa can be distinguished by RFLP analysis of the mitochondrial Cytochrome Oxydase I locus (COI).  This marker revealed strong genetic differentiation between Asian populations of the two taxa (Boudry et al., 1998). In contrast, nuclear markers showed a much lower genetic differentiation (Huvet et al, in press). The absence of any reproductive barrier...
Tipo: Text
Palavras-chave: Microsatellite; Genetic; Crassostrea angulata; Crassostrea gigas; Oysters.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3453.pdf

A first generation genetic linkage map of the European flat oyster Ostrea edulis (L.) based on AFLP and microsatellite markers
Provedor de dados: 5
Autores: Lallias, Delphine; Beaumont, A.r.; Haley, C.s.; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
This study presents the first genetic linkage map for the European flat oyster Ostrea edulis. AFLP and 20 microsatellite markers were genotyped in a three-generation pedigree comprised of 2 grand-parents, 2 parents and 92 progeny. Chi-square goodness of fit tests revealed high segregation distortion, which was significant for 32.8% of markers. Sixteen microsatellites and 235 AFLPs (170 AFLPs type 1:1 and 65 AFLPs type 3:1) were used to build sex-specific linkage maps using CriMap software. The first parental map (P1) consisted of 104 markers grouped in nine linkage groups, spanning 471.2 cM with an average spacing of 4.86 cM. The second parental map (P2) consisted of 117 markers grouped in ten linkage groups (which equals the haploid chromosome number),...
Tipo: Text
Palavras-chave: Microsatellite; AFLP; Ostrea edulis; Flat oyster; Genetic linkage map.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2642.pdf

Structuration génétique mitochondriale chez l'huître plate Ostrea edulis L. le long des côtes européennes, et comparaison avec la différentiation nucléaire
Provedor de dados: 5
Autores: Diaz Almela, Elena.
La structuration géographique des populations naturelles d'huître plate (Ostrea edulis) a été étudiée à partir de l'analyse du polymorphisme de conformation simple brin (SSCP), d'un fragment d'environ 300 pb du gène de l'ARNr mitochondrial 12S. les échantillons (13 localités, 507 individus) utilisés avaient fait l'objet d'une étude antérieure de structuration des populations avec 5 locus microsatellites. Il ont été analysés ici dans le but d'une comparaison des structurations observées sur marqueurs mitochondrial et nucléaire. La diversité moyenne intrapopulation du gène de l'ARNr 12S (Hn.b = 0,494) est presque la moitié de celle estimée avec les microsatellites (Hn.b = 0,904) bien que la variance entre populations soit beaucoup plus forte pour l'ADN...
Tipo: Text
Palavras-chave: Génétique population; Huître plate; Ostrea edulis; Microsatellite; ARNr mitochondrial 12S; Diversité génétique.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00033/14381/11671.pdf

Development of four EST-SSR multiplex PCRs in the Pacific oyster (Crassostrea gigas) and their validation in parentage assignment
Provedor de dados: 5
Autores: Li, Ronghua; Li, Qi; Cornette, Florence; Degremont, Lionel; Lapegue, Sylvie.
We report four highly informative multiplex PCRs developed from twelve previously described ESTSSRs in Crassostrea gigas. We evaluated and validated these multiplex PCRs in twelve full-sib families. The average allelic richness and the polymorphism information content (PIC) were 11.1 and 0.811 respectively. The combined power of exclusion was greater than 99.99% using all four multiplex assays. A hundred and forty three tests of segregation ratios revealed 11 significant departures from expected Mendelian ratios. The frequency of null alleles was estimated as 4.9% of all the alleles segregating based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 97% of all offspring were unambiguously...
Tipo: Text
Palavras-chave: Pacific oyster; Crassostrea gigas; Microsatellite; EST; Multiplex PCR; Parentage assignment.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00013/12419/9999.pdf

Population Genetic Structure is Unrelated to Shell Shape, Thickness and Organic Content in European Populations of the Soft-Shell Clam Mya Arenaria
Provedor de dados: 5
Autores: De Noia, Michele; Telesca, Luca; Vendrami, David L. J.; Gokalp, Hatice K.; Charrier, Gregory; Harper, Elizabeth M.; Hoffman, Joseph I.
The soft-shell clam Mya arenaria is one of the most ancient invaders of European coasts and is present in many coastal ecosystems, yet little is known about its genetic structure in Europe. We collected 266 samples spanning a latitudinal cline from the Mediterranean to the North Sea and genotyped them at 12 microsatellite loci. In parallel, geometric morphometric analysis of shell outlines was used to test for associations between shell shape, latitude and genotype, and for a selection of shells we measured the thickness and organic content of the granular prismatic (PR), the crossed-lamellar (CL) and the complex crossed-lamellar (CCL) layers. Strong population structure was detected, with Bayesian cluster analysis identifying four groups located in the...
Tipo: Text
Palavras-chave: Mya arenaria; Soft-shell clam; Microsatellite; Population genetic structure; Phenotypic plasticity; Shell morphometrics.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00614/72600/71593.pdf

Depth-dependent gene flow in Gulf of Mexico cold seep Lamellibrachia tubeworms (Annelida, Siboglinidae)
Provedor de dados: 5
Autores: Cowart, Dominique; Halanych, Kenneth M.; Schaeffer, Stephen W.; Fisher, Charles R.
Lamellibrachia vestimentiferan tubeworms form aggregations at hydrocarbon cold seeps in the deep Gulf of Mexico (GoM), creating structures that provide living space for other fauna. In the GoM, three Lamellibrachia taxa vary in morphology and depth ranges: Lamellibrachia luymesi (300–950 m), Lamellibrachia sp. 1 (950–2,604 m), and Lamellibrachia sp. 2 (1,175–3,304 m). While Lamellibrachia sp. 2 is consistently identified as a separate species, L. luymesi and sp. 1 cannot be discriminated using barcoding markers cytochrome oxidase subunit 1 (COI) and large ribosomal subunit rDNA (16S). To determine if limited gene flow was a factor in the formation of these taxa, we employed more quickly evolving markers, including mitochondrial cytochrome B (CYTB),...
Tipo: Text
Palavras-chave: Deep sea; Hydrocarbon seep; Microsatellite; Population structure; Siboglinid; Vestimentiferan tubeworm.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00194/30494/29935.pdf

Restriction to large-scale gene flow vs. regional panmixia among cold seep Escarpia spp. (Polychaeta, Siboglinidae)
Provedor de dados: 5
Autores: Cowart, Dominique; Huang, Chunya; Arnaud-haond, Sophie; Carney, Susan L.; Fisher, Charles R.; Schaeffer, Stephen W.
The history of colonization and dispersal in fauna distributed among deep-sea chemosynthetic ecosystems remains enigmatic and poorly understood because of an inability to mark and track individuals. A combination of molecular, morphological and environmental data improves understanding of spatial and temporal scales at which panmixia, disruption of gene flow or even speciation may occur. Vestimentiferan tubeworms of the genus Escarpia are important components of deep -sea cold seep ecosystems, as they provide long-term habitat for many other taxa. Three species of Escarpia, Escarpia spicata [Gulf of California (GoC)], Escarpia laminata [Gulf of Mexico (GoM)] and Escarpia southwardae (West African Cold Seeps), have been described based on morphology, but...
Tipo: Text
Palavras-chave: Deep sea; Hydrocarbon seep; Microsatellite; Population structure; Siboglinid; Vestimentiferan tubeworm.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00152/26354/24515.pdf

Contrasted levels of genetic diversity in a benthic Mediterranean octocoral: Consequences of different demographic histories?
Provedor de dados: 5
Autores: Masmoudi, Moutassem Billah; Chaoui, Lamya; Topu, Nur Eda; Hammami, Pachka; Kara, Mohamed Hichem; Aurelle, Didier.
Understanding the factors explaining the observed patterns of genetic diversity is an important question in evolutionary biology. We provide the first data on the genetic structure of a Mediterranean octocoral, the yellow gorgonian Eunicella cavolini, along with insights into the demographic history of this species. We sampled populations in four areas of the Mediterranean Sea: continental France, Algeria, Turkey, and the Balearic and Corsica islands. Along French coasts, three sites were sampled at two depths (20 and 40 m). We demonstrated a high genetic structure in this species (overall F-ST = 0.13), and most pairwise differentiation tests were significant. We did not detect any difference between depths at the same site. Clustering analyses revealed...
Tipo: Text
Palavras-chave: Octocoral; Conservation Genetics; Demographic history; Mediterranean Sea; Eunicella cavolini; Microsatellite.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00375/48587/48919.pdf

Genetic linkage mapping in the blue mussel Mytilus edulis and the European flat oyster Ostrea edulis, and the search for Quantitative Trait Loci of resistance to a disease in O. edulis.
Provedor de dados: 5
Autores: Lallias, Delphine.
Genetic linkage maps consist of ordering molecular markers across the genome and require a high number of markers for a good coverage of the genome. Such maps represent a framework which enables the identification and localisation of Quantitative Trait Loci (QTLs) for traits of interest, such as growth or disease resistance, with the final aim of achieving genetic improvement through marker-assisted selection (MAS). Data on bivalves are scarce. No genetic map has yet been constructed in any mussel or flat oyster species. We report construction of a preliminary genetic linkage map in the blue mussel, Mytilus edulis (n=14). Amplified Fragment Length Polymorphism (AFLP) markers were used in a mapping family containing 86 full-sibs issued from a controlled...
Tipo: Text
Palavras-chave: Microsatellite; AFLP; Mussel; Flat oyster; QTL; Genetic mapping; Microsatellite; AFLP; Moule; Huître plate; QTL; Cartographie génétique.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/these-2603.pdf

Genetic differences for behaviour in juveniles from two strains of brown trout suggest an effect of domestication history
Provedor de dados: 5
Autores: Benhaim, David; Guyomard, Rene; Chatain, Beatrice; Quillet, Edwige; Begout, Marie-laure.
Because captivity constitutes a drastic environmental change, domestication is expected to induce a rapid genetic selection for behavioural traits. In this study, we searched for genetic differences in behaviour among brown trout juveniles from two strains differing for their domestication history, i.e. an almost pure native wild Mediterranean population (W) and an Atlantic domesticated strain (D). In order to assess pure genetic differences among strains, males from the two origins were mated with Mediterranean females to produce two experimental crosses (WW and WD). The swimming activity characteristics of individual WW and WD juveniles were compared before and after the application of a stress (light switched off suddenly, followed by a 5-min period of...
Tipo: Text
Palavras-chave: Domestication; Genotyping; Microsatellite; Swimming activity; Restocking.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00152/26301/24409.pdf

Ressources génétiques chez l'huître creuse Crassostrea gigas : variabilité, différenciation et adaptation aux conditions de production en Charente Maritime
Provedor de dados: 5
Autores: Huvet, Arnaud; Boudry, Pierre; Baud, Jean-pierre; Nourry, Max; Phelipot, Pascal; Gerard, Andre; Ledu, Christophe; Heurtebise, Serge; Cornette, Florence; Blouin, Frederic; Bedier, Edouard; Lapegue, Sylvie.
es marqueurs génétiques, particulièrement bien adaptés à ces problématiques car hypervariables, ont été mis au point récemment dans notre laboratoire en collaboration avec le laboratoire Génome et Populations de Montpellier (CNRS). Ils permettent désormais l'analyse approfondie et la discrimination fine de populations d'huîtres creuses Crassostrea gigas et de rechercher si certaines d'entre-elles n'auraient pas des caractéristiques intéressantes pour l'ostréiculture locale (croissance, résistance estivale, affinage ... ). La diffusion de telles populations auprès de la profession serait plus rapide car il s'agit de la même espèce. Selon les recommandations du CIEM, il faut effectuer une mise en quarantaine des huîtres importées (après contrôles sanitaires)...
Tipo: Text
Palavras-chave: Génétique; Génétique des populations; Marqueurs génétiques; Microsatellite; Huître creuse; Crassostrea gigas.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00044/15563/12952.pdf

Genetic diversity, multiplicity of infection and population structure of Schistosoma mansoni isolates from human hosts in Ethiopia
Provedor de dados: 5
Autores: Aemero, Mulugeta; Boissier, Jerome; Climent, Deborah; Mone, Helene; Mouahid, Gabriel; Berhe, Nega; Erko, Berhanu.
Background: Human intestinal schistosomiasis caused by Schistosoma mansoni and urinary schistosomiasis caused by Schistosoma haematobium are endemic in Ethiopia. Although schistosomes look morphologically uniform, there is variation in infectivity, egg productivity and virulence due to variation in their genetic make. Knowing the genetic diversity and population structure of S. mansoni isolates will enable to understand and consider the possible variability in terms of infectivity, egg productivity and virulence. Methods: Between 2010 and 2011, genetic diversity and population structure of Schistosoma mansoni isolates from four endemic areas of Ethiopia was assessed using previously published 11 polymorphic microsatellite loci. Miracidia were hatched from...
Tipo: Text
Palavras-chave: Schistosoma mansoni; Microsatellite; Genetic diversity; Population structure; Ethiopia.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00302/41369/71868.pdf

Identifying the viral genes encoding envelope glycoproteins for differentiation of Cyprinid herpesvirus 3 isolates
Provedor de dados: 5
Autores: Han,; Kim, Ji Hyung; Renault, Tristan; Choresca, Casiano, Jr.; Shin, Sang Phil; Jun, Jin Woo; Park, Se Chang.
Cyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116). In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA,...
Tipo: Text
Palavras-chave: Cyprinid herpes virus 3; Glycoprotein gene; Koi herpesvirus; Microsatellite; Polymorphism; Phylogenetic tree.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00118/22940/20768.pdf

Restricted dispersal of the reef fish Myripristis berndti at the scale of the SW Indian Ocean
Provedor de dados: 5
Autores: Muths, Delphine; Tessier, Emmanuel; Gouws, G.; Craig, M.; Mwale, M.; Mwaluma, J.; Mwandya, A.; Bourjea, Jerome.
The reef fish Myripristis berndti (Jordan &amp; Everman 1903) is a pantropical species. A genetic analysis was conducted on 353 individuals from 10 localities distributed across the SW Indian Ocean (SWIO) in order to determine patterns of connectivity in the SWIO. Both the mtDNA sequences (711-bp cytochrome b sequences) and the microsatellites (8 newly developed loci) reveal spatial patterns of differentiation within the SWIO. There is, however, a discrepancy between the structure observed with each kind of marker. MtDNA revealed that 3 peripheral populations (NW Kenya, SE Reunion, and SW Europa) were isolated from the 7 more central populations, which form a more densely connected population network, while microsatellite data indicated a more restricted...
Tipo: Text
Palavras-chave: MtDNA; Microsatellite; Marine fish; SW Indian Ocean; Connectivity.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00073/18444/15974.pdf

Microsatellites development in Ostrea edulis and Mytilus edulis
Provedor de dados: 5
Autores: Lallias, Delphine; Stockdale, Ruth; Lapegue, Sylvie; Boudry, Pierre; Beaumont, Andy.
The European flat oyster Ostrea edulis and the blue mussel Mytilus edulis are both very valuable commercial species across Europe. Despite their economical importance, only a few microsatellite markers have so far been developed in those species. The aim of this study was to develop new microsatellites for O. edulis and M. edulis that could be used for population genetics studies, genetic variability assessment of stocks, parentage analysis or genetic and QTL mapping studies. For Ostrea edulis, an enriched library was made by ecogenics GmbH (Zurich, Switzerland) from size selected genomic DNA ligated into SAULA/SAULB-linker and enriched by magnetic bead selection with biotin-labelled (GT)13 and (CT)13 oligonucleotide repeats (Gautschi et al. 2000a,b). Of...
Tipo: Text
Palavras-chave: M13 tail; Blue mussel; European flat oyster; Microsatellite.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4536.pdf

Additional results and discussion to the paper: Asymmetric viability of reciprocal-cross hybrids between crested and marbled newts (Triturus cristatus and T. marmoratus)
Provedor de dados: 16
Autores: Arntzen, J.W.; Jehle, R.; Bardakci, F.; Burke, T.; Wallis, G.P.
Hybridization between divergent lineages often results in reduced hybrid viability. Here we report findings from a series of independent molecular analyses over several seasons on four life stages of F1 hybrids between the newts Triturus cristatus and T. marmoratus. These two species form a bimodal hybrid zone of broad overlap in France, with F1 hybrids making up about 4% of the adult population. We demonstrate strong asymmetry in the direction of the cross, with one class (cristatus-mothered) making up about 90% of F1 hybrids. By analyzing embryos and hatchlings, we show that this asymmetry is not due to prezygotic effects, as both classes of hybrid embryos are present at similar frequencies, implicating differential selection on the two hybrid classes...
Tipo: Article / Letter to the editor
Palavras-chave: Allozyme; Bateson–Dobzhansky–Muller incompatibility; Cytonuclear incompatibility; Hybridization; Microsatellite; MtDNA; Postzygotic isolation asymmetry; Triturus; 42.82.
Ano: 2008
URL: http://www.repository.naturalis.nl/record/289893

Evidence of multiple paternity and cooperative parental care in the so called monogamous silver arowana Osteoglossum bicirrhosum (Osteoglossiformes: Osteoglossidae)
Provedor de dados: 83
Autores: Verba,Júlia Tovar; Rabello Neto,José Gurgel; Zuanon,Jansen; Farias,Izeni.
Monogamy is rare in fishes and is usually associated with elaborate parental care. When parental care is present in fishes, it is usually the male that is responsible, and it is believed that there is a relationship between the high energetic investment and the certainty of paternity (except in the case of sneaker males). Osteoglossum bicirrhosum is considered a monogamous fish, and has particular behavioral traits that permit the study of mating systems and parental care, such as male mouthbrooding. We investigated the genetic relationships of males with the broods found in their oral cavities in Osteoglossum samples collected in a natural environment in the lower Purus river basin, Amazonas, Brazil. Fourteen broods were analyzed for parentage (268 young...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cooperative parental care; Microsatellite; Monogamy; Mouthbrooding.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000100145

Genetic diversity of the species Leporinus elongatus (Teleostei: Characiformes) in the Canoas Complex - Paranapanema River
Provedor de dados: 83
Autores: Ramos,Juliano Vilas Boas; Sodré,Leda Maria Koelblinger; Orsi,Mário Luís; Almeida,Fernanda Simões de.
Dams constructed along waterways interrupt the dispersion and migration of aquatic organisms, affecting mainly the abundance of migratory fish species. Translocation mechanisms have been constructed at dams aiming to minimize their impact on fish species migration behavior. There is little information available about the effect of the construction of dams on the genetic structure of the Neotropical migratory fish fauna. Therefore, RAPD molecular markers and microsatellites were utilized to evaluate the diversity and genetic structure of the migratory species Leporinus elongatus (piapara) in the Canoas Complex - Paranapanema River - Brazil. Ten groups were sampled in the fish ladders of the hydroelectric dam Canoas I and Canoas II during the reproductive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dams; Fish ladder; Genetic structure; Microsatellite; RAPD.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000400015

The roles of marginal lagoons in the maintenance of genetic diversity in the Brazilian migratory fishes Prochilodus argenteus and P. costatus
Provedor de dados: 83
Autores: Melo,Bruno F.; Sato,Yoshimi; Foresti,Fausto; Oliveira,Claudio.
The rio São Francisco basin contains many endemic species, such as Prochilodus argenteus and P. costatus, which have great commercial importance. However, information about the main recruitment sites and genetic studies containing extensive sampling of these species are scarce. To investigate the roles of the marginal lagoons in the maintenance of genetic variability and in the population structure, we analyzed six microsatellite loci in nine sampling groups of P. argenteusand five sampling groups of P. costatus. Our results showed high levels of genetic variability and low values of genetic differentiation for P. argenteus (FST =0.008, P&lt; 0.05) and for P. costatus(FST =0.031, P &lt; 0.05). In addition, high values of gene flow combined with a small...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Fish migration; Microsatellite; Nursery area; Rio São Francisco basin.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300625

Mating system parameters in a high density population of andirobas in the Amazon forest
Provedor de dados: 86
Autores: Campos,Tatiana de; Cunha,Marcia Oliveira da; Sousa,Adna Cristina Barbosa de; Teixeira,Renata Beltrão; Raposo,Andrea; Sebbenn,Alexandre Magno; Wadt,Lucia Helena de Oliveira.
The objective of this work was to estimate the mating system parameters of a andiroba (Carapa guianensis) population using microsatellite markers and the mixed and correlated mating models. Twelve open‑pollinated progeny arrays of 15 individuals were sampled in an area with C. guianensis estimated density of 25.7 trees per hectare. Overall, the species has a mixed reproductive system, with a predominance of outcrossing. The multilocus outcrossing rate (t m = 0.862) was significantly lower than the unity, indicating that self‑pollination occurred. The rate of biparental inbreeding was substantial (t m ‑ t s = 0.134) and significantly different from zero. The correlation of selfing within progenies was high (r s = 0.635), indicating variation in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carapa guianensis; Allogamy; Conservation; Genetic breeding; Microsatellite; Population size.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000500006

Genetic variability of marine shrimp in the Brazilian industry
Provedor de dados: 86
Autores: Maggioni,Rodrigo; Coimbra,Maria Raquel Moura; Costa,Raimundo Bezerra da; Diniz,Fábio Mendonça; Molina,Wagner Franco; Oliveira,Diana Magalhães de; Puchnick-Legat,Angela.
The objective of this work was to estimate the genetic variability level and distribution in Brazilian broodstocks of marine shrimp (Litopenaeus vannamei). Nine of the country's largest hatcheries were evaluated using codominant and highly polymorphic microsatellite markers. The results obtained from genotyping of ten microsatellite loci are indicative of genetic variability that is compatible with that found in wild populations of L. vannamei in Mexico and Central America. A possible explanation is the highly diversified and relatively recent origin of the available broodstocks. Bayesian analysis detected a signal for five founding populations. The distribution of genetic distances partially reflects geographical location, and this information will be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Litopenaeus vannamei; Aquaculture; Microsatellite; Shrimp farming.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800026

Optimal use of SSR markers for varietal identification of upland cotton
Provedor de dados: 86
Autores: Pereira,Guilherme da Silva; Cazé,Ana Luíza Ramos; Silva,Michelle Garcia da; Almeida,Vanessa Cavalcante; Magalhães,Fernanda Oliveira da Cunha; Silva Filho,João Luís da; Barroso,Paulo Augusto Vianna; Hoffmann,Lúcia Vieira.
Abstract: The objective of this work was to identify polymorphic simple sequence repeat (SSR) markers for varietal identification of cotton and evaluation of the genetic distance among the varieties. Initially, 92 SSR markers were genotyped in 20 Brazilian cotton cultivars. Of this total, 38 loci were polymorphic, two of which were amplified by one primer pair; the mean number of alleles per locus was 2.2. The values of polymorphic information content (PIC) and discrimination power (DP) were, on average, 0.374 and 0.433, respectively. The mean genetic distance was 0.397 (minimum of 0.092 and maximum of 0.641). A panel of 96 varieties originating from different regions of the world was assessed by 21 polymorphic loci derived from 17 selected primer pairs....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gossypium hirsutum; Cultivar discrimination; Intraspecific polymorphism; Microsatellite.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000700571

Polymorphism in Litopenaeus vannamei genes and cross-species amplification in other shrimp species
Provedor de dados: 86
Autores: Santos,Camilla Alves; Farias,Flavio; Teixeira,Ana Karina; Rocha,João Luís; Guerrelhas,Ana Carolina; Freitas,Patrícia Domingues de.
Abstract: The objective of this work was to assess polymorphisms within Litopenaeus vannamei candidate genes related to growth performance, and a possible association between weight genotypes and phenotypes in two shrimp closed lines. Three EST-SSR (serine-arginine, 60S ribosomal, and troponin) and one EST-SNP (crustacyanin) were selected and evaluated in L. vannamei and seven other marine shrimp species, besides two freshwater species. Genotypes had no effect on total body weight. Although no association was observed in the sampled population, these markers showed amplification for most of the evaluated species and, therefore, may be useful in further genetic studies on shrimp species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candidate genes; Crustacyanin; Microsatellite; SNP; Weight-association; 60S ribosomal.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100121

Mapping of quantitative trait loci for thermosensitive genic male sterility in indica rice
Provedor de dados: 86
Autores: Alcochete,Antonio Alberto Neves de; Rangel,Paulo Hideo Nakano; Ferreira,Marcio Elias.
The objective of this work was to select and use microsatellite markers, to map genomic regions associated with the genetic control of thermosensitive genic male sterility (TGMS) in rice. An F2 population, derived from the cross between fertile and TGMS indica lines, was used to construct a microsatellite-based genetic map of rice. The TGMS phenotype showed a continuous variation in the segregant population. A low level of segregation distortion was detected in the F2 (14.65%), whose cause was found to be zygotic selection. There was no evidence suggesting a cause-effect relationship between zygotic selection and the control of TGMS in this cross. A linkage map comprising 1,213.3 cM was constructed based on the segregation data of the F2 population....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa; Microsatellite; Hybrid rice; Genetic map.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001200004

Association between microsatellites and resistance to Mal de Río Cuarto in maize by discriminant analysis
Provedor de dados: 85
Autores: Bonamico,NC; Balzarini,MG; Arroyo,AT; Ibañez,MA; Díaz,DG; Salerno,JC; Di Renzo,MA.
Resistance to Mal de Río Cuarto (MRC) disease in maize (Zea mays L.) is important in Argentina because the crop area involves a wide region where the disease is endemic. Molecular marker-assisted selection could be used as an additional selection tool to enhance precision of the genotype selection for resistance. It demands the identification of informative markers. Microsatellite (SSR) markers linked to gene(s) associated with resistance to MRC have been reported from previous QTL analyses. These analyses have been made on linkage maps derived from a relatively early mapping population. In advanced populations, where highly distinct genotypes are easily classified, discriminant analysis (DA) represents a complementary strategy to marker identification;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maize; Microsatellite; Mal de Río Cuarto; Discriminant analysis.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000100006

Mating system in Myracrodruon urundeuva (Anarcadiaceae): implications for conservation genetics
Provedor de dados: 88
Autores: Gaino,Ana Paula Silva Campos; Moraes,Mario Luiz Teixeira de; Moreira,Juliana Prado; Cardin,Laila Toniol; Moraes,Marcela Aparecida; Silva,Alexandre Marques da; Freitas,Miguel Luiz Menezes; Sebbenn,Alexandre Magno.
The aims of this study were to investigate the mating system of a fragmented population of the dioecious tropical tree Myracrodruon urundeuva Allemão, using five microsatellite loci and the mixed mating and correlated mating models. The study was conducted in the Estação Ecológica de Paulo de Farias (436 ha), where the population occupies about 142 ha. The mating system was estimated using 514 open-pollinated offspring, collected from 30 seed-trees. Estimates of the multilocus outcrossing rate confirm that the species is dioecious (t m = 1.0). Low levels of mating among relatives were detected in the population (1 - t s = 0.020). The estimate of paternity correlation (r p(m)) indicated that offsprings were composed of mixtures of half-sibs and full-sibs,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Correlated matings; Fragmentation; Microsatellite; Variance effective population size.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042011000400008

Characterization of Hilly Chickens in Consideration of Climate Change Factors: Light and Heat
Provedor de dados: 89
Autores: Khan,MKI; Ali,M; Akter,MA; Zaman,S; Miah,G.
ABSTRACT Hilly chickens were characterized from January 2015 to September 2016 considering climate factors (light and heat). The experimental birds were divided into three groups (heat stress; light and control). The heat was generated in the chicken’s shelter by a black shaded light (Lantern) for two to three hours more after sunset. A lighting device (Lantern) was kept in the chicken’s shelter for the same period for increasing daylight duration. The individual chicken’s egg production, egg weight and mature live weight were studied from onset of egg production. It was observed that reddish brown hilly type chicken produced more (100.80 no/year/chicken) and larger sized (43.21g) eggs than spotted chicken (83.4 no/year/chicken and 40.46g). Among the three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Climatic factors; Microsatellite; Genetic diversity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400731

Genetic diversity of four cattle breeds using microsatellite markers
Provedor de dados: 96
Autores: Machado,Marco Antonio; Schuster,Ivan; Martinez,Mário Luiz; Campos,Ana Lúcia.
Four cattle breeds (Gyr, Nellore, Guzerat and Holstein) were analyzed by amplification of genomic DNA using microsatellite loci to evaluate the genetic diversity within and among them. DNA samples of 18 animals from each breed were collected to access the genetic content of them. Allele frequencies were calculated and used to generate a Nei's genetic distance matrix what was used to build a dendrogram following UPGMA clustering. As expected, Holstein breed was the most distinct from the other breeds: 1.15 in relation to Gyr, 1.12 in relation to Nellore and 0.94 in relation to Guzerat. The closest genetic distance was 0.25 between Guzerat and Nellore. A total of 64 alleles in all four breeds were detected using nine microsatellite primers. Each breed showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; DNA; Genetic diversity; Marker; Microsatellite.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982003000100012

A note on the distribution of genetic diversity of Anglo-Nubian goats in central-northern farms of Piauí, Brazil
Provedor de dados: 96
Autores: Silvestre,Ellida de Aguiar; Costa,Márcio da Silva; Silva,Pollyana Oliveira da; Bajay,Miklos Maximiliano; Pinheiro,José Baldin; Zucchi,Maria Imaculada; Campelo,José Elivalto Guimarães; Britto,Fábio Barros.
The objective of this study was to characterize the genetic variability of Anglo-Nubian goats using microsatellite markers. The study was conducted using herds from four municipalities of Central-Northern Piauí (Teresina, José de Freitas, Campo Maior, and Angical), where technical information is scarce. Seven markers suggested by FAO were used (ILSTS11, McM527, INRA23, ETH10, OarfCB304, OarfCB48, and MAF209). The samples were genotyped using a 7% polyacrylamide gel. The average number of alleles per locus was 4.0, with observed and expected heterozygosity of 0.38 and 0.55, respectively. Few deviations from the Hardy-Weinberg equilibrium were observed for each population. Only two loci deviated significantly in two localities. The coefficient of gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endogamy; Genetic structure; Microsatellite; Molecular markers.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015000400155

Genetic diversity analysis among pigeonpea genotypes adapted to South American regions based on microsatellite markers
Provedor de dados: 63
Autores: Sousa,Adna Cristina Barbosa de; Godoy,Rodolfo; Sforça,Danilo Augusto; Campos,Tatiana de; Zucchi,Maria Imaculada; Jank,Liana; Souza,Anete Pereira de.
The pigeonpea [Cajanus cajan (L) Millspaugh] is one of the most important perennial legume crops utilized in the food, fodder, soil conservation, crop-livestock integrated systems, reclaiming of degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate the genetic diversity of 77 pigeonpea genotypes selected from the germplasm collections at Embrapa Cattle-Southeast and, to evaluate their transferability to Phaseolus vulgaris and Vigna unguiculata species. The number of alleles per locus ranged from 2 to12, with an average of 5.1 alleles. The PIC values ranged from 0.11 to 0.80 (average 0.49) and the D values from 0.23 to 0.91 (average 0.58). The averages of observed and expected heterozygosity were 0.25 and 0.47,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Legumes; Transferability; Microsatellite; Germplasm.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400007

Assessment of inter- and intra-cultivar variations in olive using SSR markers
Provedor de dados: 63
Autores: Ipek,Ahmet; Barut,Erdogan; Gulen,Hatice; Ipek,Meryem.
Olive (Olea europaea L.) production in the world has been made by using many cultivars, and the genetic uniformity of commercial cultivars is important for standard olive oil and table olive production. The genetic variation among and within commonly cultivated olive cultivars in Turkey was analyzed using SSR markers. A total of 135 leaf samples were collected from 11 commonly cultivated olive cultivars from 11 provinces in four geographical regions of Turkey. Seven SSR primer pairs generated 46 SSR markers, and the number of SSR markers per primer pair ranged from 4 (UDO-14) to 9 (GAPU-89) with an average of 6.57. This high level of SSR polymorphism suggests that olive production in Turkey has been made using genetically diverse olive cultivars and this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olea europaea L.; Microsatellite; Intra-varietal variation; Synonyms; Simple sequence repeats.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000500007

Genetic similarity among Tunisian cultivated olive estimated through SSR markers
Provedor de dados: 63
Autores: Abdelhamid,Sofiane; Grati-kamoun,Naziha; Marra,Francesco; Caruso,Tiziano.
Olive (Olea europaea L. subsp. europaea var. europaea) is one of the oldest fruit tree in the Mediterranean basin, and is cultivated for oil and canned fruit. Part of this interest is driven by the economic importance of olive oil which is increasing throughout the world due to its beneficial effect to human health. In Tunisia, olive has great socio-economic importance, with more than 60 millions olive trees cultivated for olive oil production including a wide range of cultivars which are widely extended from the north to the south regions of the country for its high economic value. Here, we applied microsatellites (SSRs) molecular markers to assess the genetic variability of the most important Tunisian olive cultivars. In total, the 10 simple sequence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olea europaea; Genetic diversity; Microsatellite; Genetic relationships.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000100006

GENETIC VARIABILITY OF THE ZEBU CATTLE BREED (Bos indicus) IN THE DEPARTAMENT OF HUILA, COLOMBIA USING MICROSATELLITE MOLECULAR MARKERS
Provedor de dados: 37
Autores: HERNÁNDEZ ESCOBAR,CARLOS; OLIVERA ÁNGEL,MARTHA; OSTOS ALFONSO,HENRY; GUERRA,MARIA TERESA.
The polymorphism of 11 microsatellites from zebu cattle (Bos indicus) was studied using a commercial multiplex system to estimate genetic variability. Allele frequencies polymorphism information content and heterozygosis were calculated. Allele frequencies revealed that in the analyzed sample the markers were not equally polymorphic. The average allele was 14.2 with the highest values for the TGLA122 microsatellites. The mean heterozygocity was 0.7056 and the polymorphism information content was 0.668. This multiplex analysis could be used for pedigree information and for adequate genetic improvements in breeding programs and paternity test.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alleles; Microsatellite; Polymorphism; Zebu.
Ano: 2009
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2009000300013

Species boundary and extensive hybridization and introgression in Petunia
Provedor de dados: 39
Autores: Turchetto,Caroline; Schnitzler,Carolina K.; Freitas,Loreta B.
ABSTRACT Studying the role of hybridization in the speciation of plants is one of the most thrilling areas of evolutionary biology. Hybridization in natural populations can act in opposition to divergence, contribute to adaptation through introgression or foster the emergence of new lineages via hybrid speciation. Species of the plant genus Petunia grow in open areas in southern South America. Some natural interspecific hybrid events have been described for the genus, such as between the endemic P. exserta and the widespread P. axillaris. Both species occur in sympatry in Serra do Sudeste (Brazil), where they occur in diverse habitats and exhibit floral divergence, which has been related to the attraction of different primary pollinators. The present study...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene exchange; Hybridization; Inbreeding; Introgression; Microsatellite; Petunia; Species limits.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000400724

Cytogenetic and molecular characterization of Speothos venaticus specimens - doi: 10.4025/actascibiolsci.v32i4.6775
Provedor de dados: 3
Autores: Carvalho, Thaís Lima; UNESP - IBB; Curi, Rogério Abdallah; UNESP - FMVZ; Santiloni, Valquíria; UNESP - IBB; Chieregatto, Cleyde Angélica Ferreira da Silva; Sociedade dos Zoológicos do Brasil; Rocha, Guaracy Tadeu; UNESP - IBB; Mota, Lígia Souza Lima Silveira da; UNESP - IBB.
The bush dog (Speothos venaticus) is a South American canid, included in the IBAMA (Brazilian Institute of Environment and Renewable Natural Resources) official list of animals threatened with extinction, in the vulnerable category. As a preservation and conservation strategy, specimens kept in captivity by Brazilian Institutions are monitored by a management plan. In order to characterize and analyze the genetic variability of bush dog specimens, a cytogenetic analysis was carried out, and microsatellite data were also obtained through the use of 15 primers, originally developed for the domestic dog (Canis familiaris). All tested primers showed transferability and amplified fragment sizes similar to those described for the canine genome. From the total...
Palavras-chave: 2.02.04.00-0 bush dog; Canids; Chromosome; Microsatellite; Mosaicism Genética Animal bush dog; Canids; Chromosome; Microsatellite; Mosaicism.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6775

Migratory connections among breeding grounds off the Eastern Pacific and feeding areas in the Antarctic Peninsula based on genotype matching
Provedor de dados: 50
Autores: Caballero,Susana; Steel,Debbie; Pallin,Logan; Botero-Acosta,Natalia; Felix,Fernando; Olavarría,Carlos; Diaz granados,Maria Claudia; Bessudo,Sandra; Friedlander,Ari; Scott Baker,C.
ABSTRACT We analysed 114 DNA samples collected in different locations within the stock G breeding grounds, including Ecuador and the northern and southern Pacific of Colombia. We genotyped 15 microsatellite loci, sexed all samples, and performed genotype comparisons. Genotype comparisons were done using a DNA register of previously genotyped individuals from the Colombian breeding ground and the Antarctic Peninsula feeding Area. We confirmed connectivity between feeding grounds in Antarctic Peninsula and breeding grounds in Colombia and Ecuador using microsatellite loci. We found recaptures within each sampling location in the same year, but we also found a few recaptures in the same locations between years both in breeding and feeding grounds....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Megaptera novaeangliae; Southeastern Pacific; Genotyping; Microsatellite; Migratory connectivity.
Ano: 2021
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612021000300031

Utilization of microsatellites for the analysis of genomic alterations in colorectal cancers in Brazil
Provedor de dados: 56
Autores: Fuzikawa,A.K.; Haddad,L.A.; da-Cunha-Melo,J.R.; Brasileiro-Filho,G.; Pena,S.D.J.
Two different pathogenetic mechanisms are proposed for colorectal cancers. One, the so-called "classic pathway", is the most common and depends on multiple additive mutational events (germline and/or somatic) in tumor suppressor genes and oncogenes, frequently involving chromosomal deletions in key genomic regions. Methodologically this pathway is recognizable by the phenomenon of loss of heterozygosity. On the other hand, the "mutator pathway" depends on early mutational loss of the mismatch repair system (germline and/or somatic) leading to accelerated accumulation of gene mutations in critical target genes and progression to malignancy. Methodologically this second pathway is recognizable by the phenomenon of microsatellite instability. The distinction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase chain reaction; Colorectal carcinoma; Microsatellite; P53; DCC; Microsatellite instability.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800001

Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance
Provedor de dados: 58
Autores: Reis,Vanda Renata; Antonangelo,Ana Teresa Burlamaqui Faraco; Bassi,Ana Paula Guarnieri; Colombi,Débora; Ceccato-Antonini,Sandra Regina.
Abstract Strains of Saccharomyces cerevisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cerevisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cerevisiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fermentation; Stresses; Yeasts; Microsatellite; Contaminants.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200268

Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers
Provedor de dados: 58
Autores: Brasileiro,Bereneuza Tavares Ramos Valente; Coimbra,Maria Raquel Moura; Morais Jr,Marcos Antonio de; Oliveira,Neiva Tinti de.
Fusarium solani fungus (teleomorph Haematonectria haematococca) is of relevance for agriculture, producing a disease that causes significant losses for many cultivars. Moreover, F. solani is an opportunistic pathogen to animals and humans. The complexity associated to its correct identification by traditional methods justifies the efforts of using molecular markers for isolates characterization. In this work, three PCR-based methods (one PCR-ribotyping and two PCR-fingerprinting) were used to investigate the molecular variability of eighteen F. solani isolates from four Brazilian States, collected from different substrates. Genetic analysis revealed the intraspecific variability within the F. solani isolates, without any correlation to their geographical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intron splice site; Fusarium solani; Microsatellite; PCR-fingerprinting; Ribosomal DNA.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200006

Genetic Structure and Diversity of Four Chilean Sheep Breeds
Provedor de dados: 117
Autores: de la Barra,Rodrigo; Uribe,Héctor; Latorre,Etel; San Primitivo,Fermín; Arranz,Juan.
Chilean sheep breeds have a significant founder effect since they descend from very few parents, in some cases population inbreeding has increased and in others there have been significant differentiation processes as compared to their original population. The objective of this study was to estimate the current status of genetic diversity in sheep herds, which comprise the main sheep breeds in Chile, using molecular methods. Animals from four breeding herds were sampled and analyzed. The breeds studied were Corriedale, Suffolk Down, Romney Marsh and Chilota, these are the predominant numerical pure breeds in Chile. From each breed population 40 animals were sampled. Genetic characterization was done using nine microsatellite molecular markers (SSR)...
Tipo: Journal article
Palavras-chave: Microsatellite; Diversity; Sheep; Chile.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000400016

Analysis of genetic diversity in Argentinian heterotic maize populations using molecular markers
Provedor de dados: 118
Autores: Morales,Marcelo; Decker,Viviana; Ornella,Leonardo.
Over the past three decades, traditional Argentinean Orange Flint maize cultivars have been replaced by the higher yielding U.S. Yellow Dent germplasms. However, fint cultivars are potentially resistant to biotic and/or abiotic stress. Thus, knowledge of genetic diversity and relationships among fint inbred lines would help reduce genetic vulnerability and broaden the genetic base of crops in national improvement programs. In this study, we report the analysis of 25 inbred Orange Flint germplasms and one dent using 21 microsatellite markers or Simple Sequence Repeats (SSR). The aim was to assess genetic diversity among these accessions and evaluate the usefulness of SSR markers for defning heterotic groups in temperate germplasm. Genetic diversity values...
Tipo: Journal article
Palavras-chave: Cluster analysis; Microsatellite; Zea mays.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000100015

Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines
Provedor de dados: 65
Autores: Possamai,Mari Helen Pagani; Battilana,Jaqueline; Paludo,Ediane; Herkenhoff,Marcos Edgar; Pértile,Fábio; Lima-Rosa,Carlos André da Veiga.
This study aimed to investigate the genetic variability of two Brazilian free range (Caipira) chickens lines using microsatellites analysis of ten loci. It was collected a total of 99 blood samples, which 49 were from Paraíso Pedrês (PP) and 50 were from Rubro Negra (RN) lines. The amplification of the DNA fragments was performed by polymerase chain reaction (PCR) and the genotyping was conduct using ABI 3130 sequencer. The allele number variation was among 3 (LEI0254) to 32 (LEI0212) in the PP line, and 4 (LEI0254) to 31 (LEI0212) in the RN line. The allelic average per locus was 13.3 and 13.1 in the PP and RN lines, respectively. The average observed and the expected heterozygosity were 0.650 and 0.820 in the PP line, and 0.671 and 0.804 in the RN line....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: (Caipira) broiler line; (Caipira) layer line; Microsatellite; Polymorphism.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500877

Genetics of two marine shrimp hatcheries of the Pacific white shrimp Litopenaeus vannamei (Boone, 1931) in Pernambuco, Brazil
Provedor de dados: 65
Autores: Lima,Ana Patrícia Souza de; Silva,Suzianny Maria Bezerra Cabral da; Oliveira,Karine Kelly Cavalcanti; Maggioni,Rodrigo; Coimbra,Maria Raquel Moura.
The shrimp industry has grown significantly over the past 10 years in Brazil, especially the farmed production of the exotic Pacific white shrimp, Litopenaeus vannamei. In 2004, this industry was marked by a productivity crisis, which stirred interest towards genetic improvement of shrimp stocks. Shrimp breeders importation was banned in Brazil by a govern Normative Instruction in 1997, as a sanitary precaution. Since then, broodstock replacement in hatcheries has been based on domestic stocks, raising concerns on the decline of genetic diversity and if the existing diversity would allow effective genetic improvement programs. In the present research, genetic parameters such as number of alleles, effective allele number, expected and observed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Litopenaeus vannamei; Population genetics; Genetic diversity; Microsatellite.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000200011