Consulta: subjectFacets:"Molecular markers"
Registros recuperados: 181
Data/hora: 10/06/2024 06:41:05

Caracterización molecular de Laelia halbingeriana y su propagación in vitro.
Provedor de dados: 32
Autores: Raya Montaño, Yurixhi Atenea.
Laelia halbingeriana pertenece a la familia Orchidaceae y debido a su descubrimiento relativamente reciente, no se ha caracterizado morfológica, cromosómica ni molecularmente. Con la finalidad de conocer la diversidad de esta especie y su posible relación con L. anceps, L. superbiens y L. autumnalis, la caracterización molecular  mediante el análisis de RAPDs se utilizaron 16 iniciadores, cuatro iniciadores mostraron productos de amplificación aceptable. Se observó 60.97 % de polimorfismo que denota gran variabilidad entre las especies de Laelia. Por otra parte se observó que el número cromosómico más común es de 2n=36. Además, en un tercer estudio se desarrolló una metodología para la propagación in vitro de L. halbingeriana se utilizó el medio de MS...
Palavras-chave: Laelia halbingeriana; Marcadores moleculares; Cromosomas; Micropropagación; Aclimatación; Molecular markers; Chromosomae; Micropropagation; Genética; Doctorado; Acclimation.
Ano: 2013
URL: http://hdl.handle.net/10521/2049

Caracterización morfológica, estudio molecular y determinación de azúcares en zapote mamey (Pouteria sapota Jacq. H.E. Moore &amp; Stearn).
Provedor de dados: 32
Autores: Arellano Durán, Nely.
La importancia del zapote mamey radica en el valor comercial de sus frutos por su uso en la alimentación humana debido a sus características organolépticas y nutricionales. México es centro de origen de este frutal, distribuyéndose en 15 estados de la república, su propagación ha sido por semilla por lo cual existe gran diversidad genética. El objetivo del presente estudio fue hacer la caracterización morfológica de 12 selecciones provenientes de Puebla, Veracruz y Yucatán, así como el estudio molecular mediante la técnica AFLP`s incluyendo siete materiales de Guerrero y cuatro especies de la familia de las Sapotáceas (Pouteria campechiana, P. fossicola, Manilkara zapota y Chrisophyllum cainito. También se determinó el perfil y concentración de azúcares en...
Palavras-chave: Pouteria sapota; Diversidad; Análisis de conglomerados; Marcadores moleculares; Azúcares; Estaquiosa; Diversity; Cluster analysis; Molecular markers; Sugar; Stachyose; Fruticultura; Maestría.
Ano: 2012
URL: http://hdl.handle.net/10521/1091

Huella genética de siete razas de maíz de Valles Altos de México.
Provedor de dados: 32
Autores: Rocandio Rodríguez, Mario.
Dentro de la diversidad del maíz (Zea mays L.) de México, uno de los grupos de mayor interés es el del complejo piramidal que se cultiva en Valles Altos centrales de México. Con la finalidad de caracterizar la diversidad genética de siete razas de maiz de Valles Altos, se realizó un estudio utilizando variables morfológicas y marcadores moleculares (SSRs). Se hizo, además, un análisis simultáneo de los datos disponibles sobre dos líneas de evidencia, para hacer clasificaciones más robustas. Se incluyeron entre 8 y 26 accesiones que han sido consideradas por diferentes autores como representativas de las razas Arrocillo Amarillo, Cónicos, Elotes Cónicos, Palomero Toluqueño, Chalqueño y Purépecha. La adquisición de semillas se realizó mediante solicitud a...
Palavras-chave: Zea mays L.; Diversidad genética; Marcadores moleculares; Teocintle; Análisis conjunto; Genetic diversity; Molecular markers; Teosinte; Joint analysis; Genética; Doctorado.
Ano: 2013
URL: http://hdl.handle.net/10521/2092

Molecular description and similarity relationships among native germplasm potatoes (Solanum tuberosum ssp. tuberosum L.) using morphological data and AFLP markers
Provedor de dados: 69
Autores: Solano Solis,Jaime; Morales Ulloa,Daniza; Anabalón Rodríguez,Leonardo.
Chile is considered to be a sub-center of origin for the cultivated potato, with native and introduced genetic material coexisting in the country. Thus, the different varieties present in Chiloe Island are characterized by a rich diversity of forms, sizes, colours and phenological characteristics. In the present work, the level of polymorphism and the genetic relationship were studied by means of molecular markers using the amplified fragment length polymorphism (AFLP) technique and twenty-seven morphological characters. Twenty varieties of potatoes from the Chiloe Island were analyzed. The commercial variety Desirée and one specie from the Etuberosa series, Solanum fernandezianum, collected in the Juan Fernandez Island were included as controls. A...
Tipo: Journal article
Palavras-chave: Cluster analysis; Molecular markers; Solanum tuberosum ssp. tuberosum.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300011

A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis
Provedor de dados: 69
Autores: Sarwat,Maryam; Singh Negi,Madan; Lakshmikumaran,Malathi; Kumar Tyagi,Akhilesh; Das,Sandip; Shankar Srivastava,Prem.
For studying genetic diversity in natural populations of Terminalia, a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to isolate DNA from Terminalia arjuna. The DNA isolated using this standardized protocol was high in quality and suitable for restriction digestion and generation of random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).
Tipo: Journal article
Palavras-chave: DNA extraction; Medicinal plants; Molecular markers; Terminalia species.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100011

RAPD and freezing resistance in Eucalyptus globulus
Provedor de dados: 69
Autores: Fernández R.,Marta; Valenzuela A.,Sofía; Balocchi L.,Claudio.
Eucalyptus globulus is the second most important forest species in Chile, after Pinus radiata. The main advantages of E. globulus are its fast growth (25 m³/ha/year) and its excellent wood quality for kraft pulp production. On the negative side, its low freezing tolerance has been an obstacle for the expansion of plantations, specifically on the foothills of the Andes. The difference in the freezing resistance between clones of E. globulus has a genetic base and, therefore, it could be detected through DNA molecular markers. Fifteen clones of E. globulus, eight classified as freezing resistant and seven as freezing sensitive were analyzed using the technique of RAPD, in order to obtain molecular markers that could differentiate between freezing sensitive...
Tipo: Journal article
Palavras-chave: Eucalyptus globulus; Freezing resistance; Molecular markers; RAPD.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300025

Genetic diversity of breeding popcorn lines determined by SSR markers
Provedor de dados: 69
Autores: Ribeiro Trindade,Ana Paula; Barth Pinto,Ronald José; Amaral Júnior,Antonio Teixeira do; Mangolin,Claudete Aparecida; Silva Machado,Maria de Fátima Pires da; Scapim,Carlos Alberto.
Information about genetic dissimilarity is very important to corroborate genealogical relationships and to predict the most heterozygotic hybrid combinations. Eight popcorn S6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers. Of a total of 51 evaluated polymorphic primers, 15 were used for polymerase chain reaction (PCR) amplification. The genetic distance was estimated by Rogers&amp;#8217; modified distance. The different popcorn breeding programs in Brazil are possibly using highly similar base-populations. The genetic similarity of lines P1-3 and P8-1 was lowest, while P3-3 and P8-2 were genetically more similar. The cophenetic correlation showed that the Unweighted Pair-Group Method Using Arithmetic Averages...
Tipo: Journal article
Palavras-chave: Advanced generations; Genetic variability; Molecular markers; Zea mays.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100004

Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi
Provedor de dados: 69
Autores: Lourdes Rocha,Carla Maria; Vellicce,Gabriel Ricardo; García,María Gabriela; Pardo,Esteban Mariano; Racedo,Josefina; Perera,María Francisca; de Lucía,Adrian; Gilli,Javier; Bogado,Noelia; Bonnecarrère,Victoria; German,Silvia; Marcelino,Francismar; Ledesma,Fernando; Reznikov,Sebastián; Ploper,Leonardo Daniel; Welin,Bjorn; Castagnaro,Atilio Pedro.
Background Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. &amp;amp; Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of...
Tipo: Journal article
Palavras-chave: Asian soybean rust; Genetic variation; Glycine max; Molecular markers.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600009

Chromosomal localization of selected SCARs converted from new RAPD, ISSR and R-ISSR markers linked to rye (Secale cereale L.) tolerance to nutrient deprivation stress identified using bulk segregant analysis
Provedor de dados: 69
Autores: Smolik,Milosz.
Background: An adaptive mechanism in plant roots is initiated in the event of nitrogen and potassium deficiency, and it facilitates the active uptake of these elements in order to ensure plant growth and survival in stress conditions. Signaling and transduction of signals in response to changing nitrogen and potassium concentrations is a complex process, affected by interactions between various gene expression products, and often subjected to modifications. Results: In order to identify genotypic differences between phenotypes of two populations of recombinant inbred rye lines (153/79-1 x Ot1-3 and Ot0-6 x Ot1-3) in response to nutrition stress caused by nitrogen and potassium deficiency at the seedling stage, bulk segregant analysis was utilized....
Tipo: Journal article
Palavras-chave: Molecular markers; Nutrient deficiencies; Orthologs; Response; RILs; Rye; TIGR; TOGA.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500001

Analysis of genetic variability by ISSR markers in Calibrachoa caesia
Provedor de dados: 69
Autores: Pérez de la Torre,Mariana; García,Martín; Heinz,Ruth; Escandón,Alejandro.
Background: Calibrachoa Cerv. (ex La Llave &amp;amp; Lexarza) is a genus of the Solanaceae family (La Llave and Lexarza, 1825). This genus has a high ornamental and economic value due to its intrinsic variability and multiplicity of flower colours. In Argentina there are eight native species, and one of them is Calibrachoa caesia. The genetic diversity among 35 accessions of C. caesia, from five departments in the province of Misiones, was analyzed using ISSR markers. Results: Thirteen ISSR primers yielded a reproducible banding pattern, with 701 amplified loci and 98% of polymorphism. The ISSR primers 5’CT, 5’CA, 5’GA, 5’GACA, 3’CAC, 3’TG and 3’TC generated 100% polymorphic patterns. The Rp values ranged from 23.20 to 10.29 for 5’GACA and 3’AG primers,...
Tipo: Journal article
Palavras-chave: Anchored microsatellites; Molecular markers; Ornamental plants.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500008

The genetic dissection of quantitative traits in crops
Provedor de dados: 69
Autores: Semagn,Kassa; Bjørnstad,Åsmund; Xu,Yunbi.
Most traits of interest in plant breeding show quantitative inheritance, which complicate the breeding process since phenotypic performances only partially reflects the genetic values of individuals. The genetic variation of a quantitative trait is assumed to be controlled by the collective effects of quantitative trait loci (QTLs), epistasis (interaction between QTLs), the environment, and interaction between QTL and environment. Exploiting molecular markers in breeding involve finding a subset of markers associated with one or more QTLs that regulate the expression of complex traits. Many QTL mapping studies conducted in the last two decades identified QTLs that generally explained a significant proportion of the phenotypic variance, and therefore, gave...
Tipo: Journal article
Palavras-chave: Association mapping; Linkage disequilibrium; Markers assisted selection; Molecular breeding; Molecular markers; Quantitative trait; QTL mapping; QTL analysis.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500016

Soft computing model on genetic diversity and pathotype differentiation of pathogens: A novel approach
Provedor de dados: 69
Autores: Gürüler,Hüseyin; Peker,Musa; Baysal,Ömür.
Background Identifying and validating biomarkers' scores of polymorphic bands are important for studies related to the molecular diversity of pathogens. Although these validations provide more relevant results, the experiments are very complex and time-consuming. Besides rapid identification of plant pathogens causing disease, assessing genetic diversity and pathotype formation using automated soft computing methods are advantageous in terms of following genetic variation of pathogens on plants. In the present study, artificial neural network (ANN) as a soft computing method was applied to classify plant pathogen types and fungicide susceptibilities using the presence/absence of certain sequence markers as predictive features. Results A plant pathogen,...
Tipo: Journal article
Palavras-chave: Computational biology; Genetic diversity; Molecular markers; Plant pathogens; Predictive information; Soft computing.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500004

Assessment of genetic diversity among Pakistani wheat (Triticum aestivum L.) advanced breeding lines using RAPD and SDS-PAGE
Provedor de dados: 69
Autores: Ahmed,Muhammad Farooq; Iqbal,Muhammad; Masood,Muhammad Shahid; Rabbani,Malik Ashiq; Munir,Muhammad.
Genetic diversity was assessed among 32 advanced wheat breeding lines included in the National Uniform Wheat Yield Trials (2006-07) of Pakistan using molecular (DNA) and biochemical (SDS-PAGE) markers. Of the 72 RAPD primers used for initial screening, 15 were found polymorphic. A total of 140 bands (61.4% polymorphic) were generated by the 15 random decamer primers. Genetic similarity coefficients ranged from 0.81 to 0.94 for rainfed and from 0.70 to 0.93 for the normal seeding date group. Cluster analysis using the unweighted pair group method of arithmetic averages (UPGMA) clustered the 32 advanced wheat breeding lines into one major and three small groups. Maximum level of polymorphism (90%) was observed for the primer OPA-05. Lines N9 and N11 showed...
Tipo: Journal article
Palavras-chave: Genetic diversity; Molecular markers; Polymorphism; RAPD; SDS-PAGE; Wheat.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300001

Genetic diversity in two variants of Orobanche gracilis Sm. [var. gracilis and var. deludens (Beck) A. Pujadas] (Orobanchaceae) from different regions of Spain
Provedor de dados: 69
Autores: Román,Belén; Hernández,Rosabell; Pujadas-Salvá,Antonio José; Cubero,José Ignacio; Rubiales,Diego; Satovic,Zlatko.
The pattern of genetic variation among populations of two Orobanche gracilis Sm. taxa (var. gracilis and var. deludens (Beck) A. Pujadas) from Northern and Southern Spain growing on different hosts was analysed using RAPD markers. The diversity analysis within populations revealed a higher level of diversity in the populations from the North when compared to the Southern ones. The results of principal co-ordinate analysis (PCoA) based on Dice distances among samples clearly established the separation of samples according to the taxonomical variety and the geographical origin of each population. The Southern populations of both var. gracilis and var. deludens were more differentiated among them than those of var. gracilis from the North. The analysis of...
Tipo: Journal article
Palavras-chave: AMOVA; Molecular markers; Orobanche gracilis; Parasitic plants; Population genetics.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200006

Extent and structure of genetic diversity in a collection of the tropical multipurpose shrub legume Cratylia argentea (Desv.) O. Kuntze as revealed by RAPD markers
Provedor de dados: 69
Autores: Andersson,Meike S; Schultze-Kraft,Rainer; Peters,Michael; Duque,Myriam C; Gallego,Gerardo.
The tropical multipurpose shrub legume Cratylia argentea is well adapted to acid soils of low to medium fertility and has excellent drought-tolerance. Due to its high nutritive value it is particularly suited as forage for dry-season supplementation. A collection of 47 C. argentea accessions in a collection, derived from seed replicating of original accessions with differing geographic origin and morphological and agronomic characteristics was investigated using molecular markers (RAPD (random amplified polymorphic DNA)). Genetic diversity (H T = 0.145) in the collection was low, with 30% of differentiation among groups and high genetic similarity among accessions (GS = 0.805). Within-accession variability was high. One taxonomic mismatch and five possible...
Tipo: Journal article
Palavras-chave: Agroforestry; Molecular markers; MPT (multipurpose shrubs and trees); Tropics.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300006

Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines
Provedor de dados: 69
Autores: González,Germán Andrés; Pacheco,María Gabriela; Oneto,Cecilia Décima; Etchart,Valeria J; Kandus,Mariana V; Salerno,Juan Carlos; Eyherabide,Guillermo; Presello,Daniel; Lewi,Dalia Marcela.
Somatic embryogenesis, which is still the method of choice for tissue culture, regeneration and transformation of maize, is largely considered highly genotype-dependent. The Hi II, a highly embryogenic genotype, has been extensively used in transformation protocols. However, this is not an inbred line; instead, it has a proportion of the undesirable A-188 background, and the progeny segregates for phenotypic characteristics and shows poor agronomic performance. In an effort to identify genotypes that combine a high somatic embryogenic response and good agronomic performance, we evaluated 48 advanced inbred lines developed at INTA. Callus development and somatic embryogenesis capacity were measured in 200 immature embryos per line. Embryogenic capacity [EC...
Tipo: Journal article
Palavras-chave: Callus; Inbred lines; Maize; Molecular markers; Regeneration; Somatic embryogenesis.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100009

Genetic variability for waxy genes in Argentinean bread wheat germplasm
Provedor de dados: 69
Autores: Vanzetti,Leonardo Sebastián; Pflüger,Laura Alicia; Rodríguez-Quijano,Marta; Carrillo,José Maria; Helguera,Marcelo.
Amylose and amylopectin are the two polysaccharides that constitute starch in bread wheat and the enzyme GBSSI (Granule-bound starch synthase I), also known as waxy protein, is responsible for amylose synthesis in storage tissues. Decrease of the amylose content in starch has been associated with the lack of waxy protein(s). In this work, different sets of PCR markers were used to characterize the genetic variability of waxy loci from 103 Argentinean bread wheat cultivars. For the Wx-A1 locus, Wx-A1a and a novel molecular allele designed Wx-A1g were detected. Wx-B1 locus showed three alleles (Wx-B1a, Wx-B1b, Wx-B1e), and Wx-D1 locus showed only the Wx-D1a allele. Novel single-locus allele specific markers for Wx-A1b, Wx-B1b and Wx-D1b null alleles were...
Tipo: Journal article
Palavras-chave: Characterization; Molecular markers; Triticum aestivum L.; Starch; Waxy genes; Wheat.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100004

Efficiency of RAPD, ISSR, AFLP and ISTR markers for the detection of polymorphisms and genetic relationships in camote de cerro (Dioscorea spp.)
Provedor de dados: 69
Autores: Velasco-Ramírez,Ana Paulina; Torres-Morán,Martha Isabel; Molina-Moret,Sandy; Sánchez-González,José de Jesús; Santacruz-Ruvalcaba,Fernando.
Background At present, species known as camote de cerro (Dioscorea spp.) are found only in the wilderness in Mexico, but their populations are extremely depleted because they are indiscriminately collected, it is urgent to evaluate the conservation status of these plants in order to design conservation genetics programs. In this study, genetic diversity parameters along with cluster analysis based on Jaccard's coefficient were estimated with the objective to assess the efficiency of Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR), Amplified Fragment Length Polymorphism (AFLP) and Inverse Sequence Tagged Repeat (ISTR) molecular DNA markers in the Dioscorea genus. Results The polymorphic information contents were quite similar...
Tipo: Journal article
Palavras-chave: Dioscoreaceae; Genetic diversity; Molecular markers; Tuber.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200002

Analysis of genetic diversity among Indian niger [Guizotia abyssinica (L. f.) Cass.] cultivars based on randomly amplified polymorphic DNA markers
Provedor de dados: 69
Autores: Nagella,Praveen; Hosakatte,Niranjana Murthy; Ravishankar,K.V.; Hahn,Eun-Joo; Paek,Kee-Yoeup.
Randomly amplified polymorphic DNA (RAPD) markers were used to estimate genetic diversity among 18 cultivars of niger from India. Total genomic DNA was extracted and subjected to RAPD analysis using 80 arbitrary 10-mer primers; 17 primers were selected, which yielded a total of 124 bands, 41.20% of them polymorphic. None of the primers produced unique banding pattern for each cultivar. RAPD data were used to calculate a Squared-Euclidean Distance matrix which revealed a minimum genetic distance between cultivars JNC-6 and N-48 and a maximum distance between IGP-76 and JN-30. Based on the distance matrix, a cluster analysis was done using a minimum variance algorithm. The dendrogram generated, based on Ward&amp;#8217;s method, grouped 18 niger cultivars...
Tipo: Journal article
Palavras-chave: Genetic diversity; Guizotia abyssinica; Molecular markers; RAPD.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100014

A molecular marker approach using intron flanking EST-PCR to map candidate genes in peach (Prunus persica)
Provedor de dados: 69
Autores: Diez-de-Medina,Sergio; Silva,Herman.
In Peach (Prunus persica) several physiological changes, such as woolliness, triggered by chilling injury are involved in major production losses due to cold storage of the fruits during shipping. Additionally, the low level of polymorphisms among peach varieties is an important limitation in the search for new molecular markers that could be associated with economically important traits. Therefore, a functional approach was employed to associate candidate genes with an informative marker in peach. The data was obtained from the results of an in silico analysis of four different cold peach treatments. Thirty two candidate genes were selected that were aligned against Arabidopsis thaliana genomic sequences to design intron-flanking EST-PCR markers. These...
Tipo: Journal article
Palavras-chave: Bin mapping; Molecular markers; Prunus; Rosaceae.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500007

Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers
Provedor de dados: 69
Autores: Vanzetti,Leonardo Sebastián; Campos,Pablo; Demichelis,Melina; Lombardo,Lucio Andres; Aurelia,Paola Romina; Vaschetto,Luis María; Bainotti,Carlos Tomás; Helguera,Marcelo.
Leaf rust, caused by Puccinia triticina Eriks. is a common and widespread disease of bread wheat (Triticum aestivum L.), in Argentina. Host resistance is the most economical, effective and ecologically sustainable method of controlling the disease. Gene postulation helps to determine leaf rust resistance genes (Lr genes) that may be present in a large group of wheat germplasm. Additionally presence of Lr genes can be determined using associated molecular markers. The objective of this study was to identify Lr genes that condition leaf rust resistance in 66 wheat cultivars from Argentina. Twenty four differential lines with individual known leaf rust resistance genes were tested with 17 different pathotypes of leaf rust collected from Argentina. Leaf rust...
Tipo: Journal article
Palavras-chave: Genetic control of pathogens; Molecular markers; Plant breeding.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300009

Assessment of somaclonal variation in somatic embryo-derived plants of yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Robinson] using inter simple sequence repeat analysis and flow cytometry
Provedor de dados: 69
Autores: Viehmannova,Iva; Bortlova,Zuzana; Vitamvas,Jan; Cepkova,Petra Hlasna; Eliasova,Katerina; Svobodova,Eva; Travnickova,Martina.
Background Yacon (Smallanthus sonchifolius) is a root crop native to the Andean region. Low sexual reproductive capacity is a major constraint facing the genetic breeding of this crop. Biotechnological techniques offer alternative ways to widen genetic variability. We investigated somaclonal variation in regenerants of yacon derived from in vitro somatic embryogenesis using simple sequence repeat (ISSR) analysis and flow cytometry. Results Twenty tested ISSR primers provided a total of 7848 bands in 60 in vitro regenerants and control plant. The number of bands for each primer varied from 3 to 10, and an average of 6.95 bands was obtained per ISSR primer. Eight primers were polymorphic and generated 10 polymorphic bands with 7.19% mean polymorphism. ISSR...
Tipo: Journal article
Palavras-chave: DNA polymorphism; Molecular markers; Ploidy level; Proembryogenic callus.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200008

Genetic diversity of Chondrostereum purpureum (Pers.) Pouzar causing silverleaf disease on blueberries in Chile
Provedor de dados: 73
Autores: Rojo,Carmen; Becerra,Viviana; France,Andrés; Paredes,Mario; Buddie,Alan; Balzarini,Monica.
ABSTRACT Blueberry (Vaccinium corymbosum L.) in Chile is being affected by silver disease whose causative agent is Chondrostereum purpureum (Pers.) Pouzar. This fungus causes a decrease in the yield of the fruit and the death of the plant. So far, this disease has not been reported in the literature attacking blueberry plants in Chile. The main objective of this research was to characterize genetically the populations of Ch. purpureum at molecular level using the ITS-RFLP markers. The results of the ITS-RFLP indicate a low level of diversity in the Chilean populations of Ch. purpureum. When analyzing the isolates collected in blueberry, two large groups were configured in the dendrogram. The first one grouped 49 isolates with the same molecular profile and...
Tipo: Journal article
Palavras-chave: Chondrostereum purpureum; ITS-RFLP diversity; Molecular markers.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432017000100176

Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers
Provedor de dados: 74
Autores: Palmieri,Darío A.; Bechara,Marcelo D.; Curi,Rogério A.; Monteiro,Jomar P.; Valente,Sérgio E.S.; Gimenes,Marcos A.; Lopes,Catalina R.
Diversity in 26 microsatellite loci from section Caulorrhizae germplasm was evaluated by using 33 accessions of A. pintoi Krapov. &amp;amp; W.C. Gregory and ten accessions of Arachis repens Handro. Twenty loci proved to be polymorphic and a total of 196 alleles were detected with an average of 9.8 alleles per locus. The variability found in those loci was greater than the variability found using morphological characters, seed storage proteins and RAPD markers previously used in this germplasm. The high potential of these markers to detect species-specific alleles and discriminate among accessions was demonstrated. The set of microsatellite primer pairs developed by our group for A. pintoi are useful molecular tools for evaluating Section Caulorrhizae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachis; Genetic diversity; Germplasm; Microsatellites; Molecular markers.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100019

Tandem repeat distribution of gene transcripts in three plant families
Provedor de dados: 74
Autores: Maia,Luciano Carlos da; Souza,Velci Queiróz de; Kopp,Mauricio Marini; Carvalho,Fernando Irajá Félix de; Oliveira,Antonio Costa de.
Tandem repeats (microsatellites or SSRs) are molecular markers with great potential for plant genetic studies. Modern strategies include the transfer of these markers among widely studied and orphan species. In silico analyses allow for studying distribution patterns of microsatellites and predicting which motifs would be more amenable to interspecies transfer. Transcribed sequences (Unigene) from ten species of three plant families were surveyed for the occurrence of micro and minisatellites. Transcripts from different species displayed different rates of tandem repeat occurrence, ranging from 1.47% to 11.28%. Both similar and different patterns were found within and among plant families. The results also indicate a lack of association between genome size...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; EST; Comparative genomics; Molecular markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400025

Molecular marker heterozygosities and genetic distances as correlates of production traits in F1 bovine crosses
Provedor de dados: 74
Autores: Tambasco-Talhari,Daniella; Alencar,Maurício Mello de; Paz,Cláudia Cristina Paro de; Cruz,Geraldo Maria da; Rodrigues,Armando de Andrade; Packer,Irineu Umberto; Coutinho,Luiz Lehmann; Regitano,Luciana Correia de Almeida.
Several studies have investigated the relationship between heterozygosity, genetic distance and production traits. The objective of the present study was to evaluate the influence of the degree of heterozygosity and genetic distance on growth, carcass and reproductive related features in F1 bovine crosses. We tested 10 polymorphic markers in 330 purebred cattle (Nelore, Canchim, Aberdeen Angus and Simental) and 256 crossbred cattle belonging to four crossbred groups. Individual heterozygosities (Hi) and multilocus genetic similarity (Dm) were estimated and used in correlation analysis against individual phenotypic measurements. Significant (p &lt; 0.05) Hi effects occurred for birth weight, 15 to 18 month weight, hot carcass weight and longissimus rib eye...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine crosses; Molecular markers; Heterozygosity; Genetic distance; Production traits.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200007

Association of the bovine major histocompatibility complex (BoLA) BoLA-DRB3 gene with fat and protein production and somatic cell score in Brazilian Gyr dairy cattle (Bos indicus)
Provedor de dados: 74
Autores: Nascimento,Carlos Souza do; Machado,Marco Antonio; Martinez,Mario Luiz; Silva,Marcos Vinícius G. Barbosa da; Guimarães,Marta Fonseca Martins; Campos,Ana Lúcia; Azevedo,Ana Luisa Sousa; Teodoro,Roberto Luiz; Verneque,Rui da Silva; Guimarães,Simone Eliza Facioni; Oliveira,Denise Aparecida Andrade.
The effect of the bovine major histocompatibility complex (BoLA) locus on animal health may be due to a direct action of its alleles on immune functions, whereas its indirect effect on production traits might be explained by the better general health conditions of more productive animals. In the present study, the BoLA-DRB3 gene was investigated in 1058 cows belonging to seven Brazilian Gyr Dairy herds (Bos indicus, Zebu cattle). A total of 37 alleles were identified, 15 of them described for the first time in a Zebu breed. A highly significant association (p &lt; 0.02) was observed between allele *54 and a decrease (-26.1 kg) in milk protein yield and there was a significant association (p &lt; 0.05) between this allele and lower (-26.07 kg) milk fat...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Marker-assisted selection; Molecular markers; PCR-RFLP.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400011

Skewed RAPD markers in linkage maps of Citrus
Provedor de dados: 74
Autores: Oliveira,Roberto Pedroso de; Aguilar-Vildoso,Carlos Ivan; Cristofani,Mariângela; Machado,Marcos Antônio.
The objective of this work was to analyze the effects of RAPD markers with skewed segregation on genetic linkage maps. Segregation data for 123 Citrus sinensis (L.) Osbeck cv. Pêra markers and 53 C. reticulata Blanco cv. Cravo markers in F1 progeny composed of 94 hybrids were used. Genetic linkage maps of the two varieties were constructed with non-skewed markers (p &lt; 0.05 and p &lt; 0.01) using the program MAPMAKER 3.0 and a pseudo-testcross strategy. The maps were compared to those constructed with all markers. Alterations in the genetic distances were observed based on the location of the skewed markers within the linkage groups. Generally, the skewed markers were located at the end of the linkage groups, sometimes forming entire linkage groups,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic distance; Mendelian segregation; Molecular markers; Segregation distortion.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300021

Genetic diversity of Hirsutella thompsonii isolates from Thailand based on AFLP analysis and partial beta-tubulin gene sequences
Provedor de dados: 74
Autores: Tigano,Myrian S.; Adams,Byron; Maimala,Saowanit; Boucias,Drion.
Amplified fragment length polymorphism (AFLP) was used to investigate polymorphism among 43 Hirsutella thompsonii isolates (33 from Thailand) obtained from various mite species. The outgroups were an unidentified Hirsutella isolate along with Hirsutella nodulosa and Hirsutella kirchneri. Phylogenetic analyses of the AFLP data showed significant variation among isolates and the existence of three H. thompsonii clades. We also investigated the isolates using PCR with specific primers for the Hirsutella exotoxin gene Hirsutelin A (HtA) and 18 of these isolates were used for sequencing of the partial beta-tubulin gene. Phylogenetic analyses of beta-tubulin sequences showed two distinct H. thompsonii clades, one of which included AFLP clades I and II. For both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogenic fungus; Mites; Molecular markers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400022

Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)
Provedor de dados: 74
Autores: Novelli,Valdenice M.; Cristofani,Mariangela; Souza,Alessandra A.; Machado,Marcos A.
The aims of this study were to develop simple sequence repeat markers (SSRs or microsatellite markers) in citrus and to evaluate the efficiency of these markers for characterization of sweet orange. We developed SSRs from a genomic library of 'Pêra IAC' sweet orange enriched for AG/TC, GT/CA, TCA/AGT and AAC/TTG sequence repeats. We selected 279 sequences from which 171 primer pairs were designed of which 113 with the best banding patterns were selected. Characterization of sweet orange microsatellite loci revealed that AG/TC was the most abundant (69%) microsatellite class isolated, followed by GT/CA (15.9%), TCA/AGT (8%) and AAC/TTG (6.2%). The number of alleles ranged from 1 to 4, with a mean of 2 alleles per locus. Four microsatellite loci developed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus species; Germplasm bank; Polymorphism; Molecular markers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100018

Mapping QTLs for drought tolerance in a SEA 5 x AND 277 common bean cross with SSRs and SNP markers
Provedor de dados: 74
Autores: Briñez,Boris; Perseguini,Juliana Morini Küpper Cardoso; Rosa,Juliana Santa; Bassi,Denis; Gonçalves,João Guilherme Ribeiro; Almeida,Caléo; Paulino,Jean Fausto de Carvalho; Blair,Matthew Ward; Chioratto,Alisson Fernando; Carbonell,Sérgio Augusto Morais; Valdisser,Paula Arielle Mendes Ribeiro; Vianello,Rosana Pereira; Benchimol-Reis,Luciana Lasry.
Abstract The common bean is characterized by high sensitivity to drought and low productivity. Breeding for drought resistance in this species involves genes of different genetic groups. In this work, we used a SEA 5 x AND 277 cross to map quantitative trait loci associated with drought tolerance in order to assess the factors that determine the magnitude of drought response in common beans. A total of 438 polymorphic markers were used to genotype the F8 mapping population. Phenotyping was done in two greenhouses, one used to simulate drought and the other to simulate irrigated conditions. Fourteen traits associated with drought tolerance were measured to identify the quantitative trait loci (QTLs). The map was constructed with 331 markers that covered all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic stress; Interpopulation gene-pool; Molecular markers; QTL mapping; Water deficit.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500813

RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean
Provedor de dados: 74
Autores: Martins Filho,Sebastião; Sediyama,Carlos Sigueyuki; Moreira,Maurilio Alves; Barros,Everaldo Gonçalves de.
The soybean (Glycine max (L.) Merrill) frogeye leaf spot is caused by the fungus Cercospora sojina Hara and is a widespread disease in Brazil and other countries, causing severe losses in grain yield and also affecting seed quality. The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated. Bulked segregant analysis was applied to 3 F2 populations derived from crosses between the resistant cultivars Parana, Cristalina and Uberaba, and the susceptible cultivar Bossier. In the cross 'Parana' x 'Bossier', 2 RAPD markers were identified, CSOPA1(800C) and CSOPA2(1,250C), located at 4.4 ± 1.8 centiMorgans (cM) and 3.4 ± 1.7 cM respectively from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; RAPD; SCAR; Soybean; Cercospora sojina; Frogeye leaf spot.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300012

Analysis of genetic diversity and population structure in Argentine and Bolivian Creole cattle using five loci related to milk production
Provedor de dados: 74
Autores: Lirón,J.P.; Ripoli,M.V.; De Luca,J.C.; Peral-García,P.; Giovambattista,G.
Data from five protein-coding loci related to dairy production were used to study the genetic diversity and population structure of Argentine and Bolivian Creole cattle breeds. Genomic DNA was extracted from blood samples of six Creole cattle breeds: Argentine (n = 230), Patagonian (n = 25); "Saavedreño" (n = 140), "Chaqueño Boliviano" (n = 30), "Yacumeño" (n = 27), and "Chusco" (n = 11). kappa-casein, beta-lactoglobulin, growth hormone and prolactin were measured by PCR-RFLP, while alphaS1-casein was typed by PCR-ASO. The results are discussed, focusing on: historical origin, recent differentiation and selection events, Zebu gene introgression, and population structure. This work shows that: (i) For the studied genes, the observed gene frequency profiles...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Creole cattle; Polymorphism; Genetic diversity; Molecular markers.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400010

Characterization and genetic diversity analysis of cotton cultivars using microsatellites
Provedor de dados: 74
Autores: Bertini,Cândida H.C. de Magalhães; Schuster,Ivan; Sediyama,Tocio; Barros,Everaldo Gonçalves de; Moreira,Maurílio Alves.
Genetic diversity and the relationship between varieties are of great importance for cotton breeding. Our work was designed to estimate the informativeness of the cotton (Gossypium hirsutum L.) simple sequence repeat (SSR) microsatellite locus and to estimate the genetic distance between 53 cotton cultivars as well as to select a set of SSR primers able to differentiate between the 53 cotton cultivars studied. After extracting DNA from the 53 cultivars and characterized it using 31 pairs of SSR primers we obtained a total of 66 alleles with an average of 2.13 alleles per SSR locus and values of polymorphism information content (PIC) varying from 0.18 to 0.62, the dissimilarity coefficient varying from zero to 0.41. Statistical analysis using the unweighted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fingerprinting; Gossypium hirsutum L.; Genealogy; Molecular markers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200021

Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines
Provedor de dados: 74
Autores: Garcia,Antonio A. F.; Benchimol,Luciana L.; Barbosa,Antônia M. M.; Geraldi,Isaias O.; Souza Jr.,Cláudio L.; Souza,Anete P. de.
In order to compare their relative efficiencies as markers and to find the most suitable marker for maize diversity studies we evaluated 18 inbred tropical maize lines using a number of different loci as markers. The loci used were: 774 amplified fragment length polymorphisms (AFLPs); 262 random amplified polymorphic DNAs (RAPDs); 185 restriction fragment length polymorphisms (RFLPs); and 68 simple sequence repeats (SSR). For estimating genetic distance the AFLP and RFLP markers gave the most correlated results, with a correlation coefficient of r = 0.87. Bootstrap analysis were used to evaluate the number of loci for the markers and the coefficients of variation (CV) revealed a skewed distribution. The dominant markers (AFLP and RAPD) had small CV values...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diversity studies; Genetic distances; Maize; Molecular markers.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400019

Characterization of Brazilian soybean cultivars using microsatellite markers
Provedor de dados: 74
Autores: Priolli,Regina Helena Geribello; Mendes-Junior,Celso Teixeira; Arantes,Neylson Eustáquio; Contel,Eucleia Primo Betioli.
Microsatellite markers or SSR (Simple Sequence Repeats) have proved to be an excellent tool for cultivar identification, pedigree analysis and the evaluation of genetic distance among organisms. Soybean cultivars have been characterized mainly by morphological and biochemical traits. However, these traits have not been sufficient to characterize the large number of cultivars eligible to receive protection under the Brazilian Cultivar Protection Act. In order to define new soybean cultivar markers, the alleles of twelve SSR loci of 186 Brazilian soybean cultivars were studied by estimating the variation in their size range and their respective frequencies. On average, 5.3 alleles per locus were detected, with a mean genetic diversity of 0.64 ± 0.12. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max (L.) Merrill; Simple sequence repeat; Microsatellites; Molecular markers; Soybean elite cultivars.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200012

Genetic polymorphisms at the leptin receptor gene in three beef cattle breeds
Provedor de dados: 74
Autores: Almeida,Sabrina E.M.; Santos,Luciana B.S.; Passos,Daniel T.; Corbellini,Ângela O.; Lopes,Beatriz M.T.; Kirst,Cláudia; Terra,Gustavo; Neves,Jairo P.; Gonçalves,Paulo B.D.; Moraes,José C.F.; Weimer,Tania de Azevedo.
The genetic diversity of a single nucleotide polymorphism (SNP) at the exon 20 (T945M) of the leptin receptor gene (LEPR) and of three short tandem repeats (STRs BM7225, BMS694, and BMS2145) linked to LEPR was investigated in three beef cattle herds (Brangus Ibagé, Charolais, and Aberdeen Angus). A cheap and effective new method to analyze the T945M polymorphism in cattle populations was developed and the possible role of these polymorphisms in reproduction and weight gain of postpartum cows was evaluated. High levels of genetic diversity were observed with the average heterozygosity of STRs ranging from 0.71 to 0.81. No significant association was detected between LEPR markers and reproductive parameters or daily weight gain. These negative results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Genetic diversity; Cattle production; LEPR gene polymorphisms.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400013

Metatranscriptomic analysis of small RNAs present in soybean deep sequencing libraries
Provedor de dados: 74
Autores: Molina,Lorrayne Gomes; Cordenonsi da Fonseca,Guilherme; Morais,Guilherme Loss de; de Oliveira,Luiz Felipe Valter; Carvalho,Joseane Biso de; Kulcheski,Franceli Rodrigues; Margis,Rogerio.
A large number of small RNAs unrelated to the soybean genome were identified after deep sequencing of soybean small RNA libraries. A metatranscriptomic analysis was carried out to identify the origin of these sequences. Comparative analyses of small interference RNAs (siRNAs) present in samples collected in open areas corresponding to soybean field plantations and samples from soybean cultivated in greenhouses under a controlled environment were made. Different pathogenic, symbiotic and free-living organisms were identified from samples of both growth systems. They included viruses, bacteria and different groups of fungi. This approach can be useful not only to identify potentially unknown pathogens and pests, but also to understand the relations that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Next generation sequencing; Small RNA; SiRNA; Molecular markers.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200010

Simulation of population size and genome saturation level for genetic mapping of recombinant inbred lines (RILs)
Provedor de dados: 74
Autores: Silva,Luciano da Costa e; Cruz,Cosme Damião; Moreira,Maurilio Alves; Barros,Everaldo Gonçalves de.
Various population sizes and number of markers have been used to obtain genetic maps. However, the precise number of individuals and markers needed for obtaining reliable maps is not known. We used data simulation to determine the influence of population size, the effect of the degree of marker saturation of the genome, and the number of individuals required for mapping of recombinant inbred lines (RILs). Three genomes with 11 linkage groups were generated with saturation levels of 5, 10 and 20 cM. For each saturation level populations were generated with 50, 100, 154, 200, 300, 500 and 800 individuals with 100 replications for each population size. A total of 2100 populations was generated and mapped. Small marker numbers and small population sizes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recombinant inbred lines; Molecular markers; Number of individuals; Computer simulation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600013

Genetic variability in Brazilian wheat cultivars assessed by microsatellite markers
Provedor de dados: 74
Autores: Schuster,Ivan; Vieira,Elisa Serra Negra; Silva,Glacy Jaqueline da; Franco,Francisco de Assis; Marchioro,Volmir Sérgio.
Wheat (Triticum aestivum) is one of the most important food staples in the south of Brazil. Understanding genetic variability among the assortment of Brazilian wheat is important for breeding. The aim of this work was to molecularly characterize the thirty-six wheat cultivars recommended for various regions of Brazil, and to assess mutual genetic distances, through the use of microsatellite markers. Twenty three polymorphic microsatellite markers (PMM) delineated all 36 of the samples, revealing a total of 74 simple sequence repeat (SSR) alleles, i.e. an average of 3.2 alleles per locus. Polymorphic information content (PIC value) calculated to assess the informativeness of each marker ranged from 0.20 to 0.79, with a mean of 0.49. Genetic distances among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Germplasm; Cultivar characterization; Cluster analysis; Molecular markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300019

5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers
Provedor de dados: 74
Autores: Alves-Costa,Fernanda A.; Martins,Cesar; Matos,Fernanda Del Campos de; Foresti,Fausto; Oliveira,Claudio; Wasko,Adriane P.
In order to extend the genetic data on the Sciaenidae fish family, the present study had the purpose to characterize PCR-generated 5S rDNA repeats of twelve species of this group through PAGE (Polyacrylamide Gel Electrophoresis) analysis. The results showed the occurrence of at least two different 5S rDNA size classes in all the species. Moreover, 5S rDNA repeats of one of the studied species - Isopisthus parvipinnis - were cloned and subjected to nucleotide sequencing and Southern blot membrane hybridization analyses, which permitted to confirm the existence of two major 5S rDNA classes. Phylogenetic analysis based on the nucleotide sequences of different 5S rDNA repeats of I. parvipinnis lead to their separation into two major clusters. These results may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sciaenidae; Fish; Isopisthus parvipinnis; 5S rDNA; Molecular markers.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200025

Heterologous microsatellite primer pairs informative for the whole genus Arachis
Provedor de dados: 74
Autores: Hoshino,Andrea Akemi; Bravo,Juliana Pereira; Angelici,Carla M.L.C.D.; Barbosa,Andréa V. Gobbi; Lopes,Catalina Romero; Gimenes,Marcos A.
The genus Arachis currently comprises 69 described species, some of which have potential and real value as human and animal foods. These Arachis species have been collected and maintained in germplasm banks to provide material that can be used as sources of genes in breeding programs and for the selection of new cultivars. One of the principal objectives of germplasm conservation is the evaluation of genetic variability, which is best conducted using molecular markers. We investigated the use of heterologous primers to amplify microsatellite loci that could be used to evaluate genetic variability in Arachis germplasm. Fifteen microsatellite primer pairs were tested in 76 accessions of 34 species from the nine Arachis sections. The data indicated that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peanut; Genetic relationship; Molecular markers; Wild relatives.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400016

Comparative linkage mapping of Oryza glumaepatula and Oryza sativa interspecific crosses based on microsatellite and expressed sequence tag markers
Provedor de dados: 74
Autores: Rangel,Priscila Nascimento; Brondani,Rosana Pereira Vianello; Coelho,Alexandre Siqueira Guedes; Rangel,Paulo Hideo Nakano; Brondani,Claudio.
Molecular linkage maps representing the rice genome have been an important tool for breeding programs because they allow the elucidation of polygenic traits and are an efficient tool for monitoring wild introgressions in interspecific crosses. Common markers among rice genetic maps are important in defining the homology of chromosomes and the synteny between genomic target regions. We used 148 markers (expressed sequence tags, microsatellites and single nucleotide polymorphisms) to construct a molecular linkage map based on co-dominant markers for an interspecific backcross population using a wild rice (Oryza glumaepatula) from Brazil and performed a comparative analysis with other interspecific maps. The comparative analysis revealed a Spearman...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comparative linkage map; Molecular markers; Oryza glumaepatula; Rice.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400019

Challenges and prospects of population genetic studies in terns (Charadriiformes, Aves)
Provedor de dados: 74
Autores: Faria,Patrícia J.; Baus,Erika; Morgante,João S.; Bruford,Michael W.
Little information is available about the population structure of communally nesting terns (Sternidae) and skimmers (Rynchopidae) throughout the world. In order to fill this gap, a survey of molecular markers was carried out for six species of terns (Anous stolidus, Sterna hirundinacea, S. fuscata, S. superciliaris, Thalasseus maximus and Phaetusa simplex) and one species of skimmer (Rynchops niger). First, we describe the results of the construction of genomic DNA libraries and document problems encountered during this procedure. Secondly, we tested the cross-amplification of 18 microsatellite loci previously described for related species (the number of polymorphic loci ranged from three to seven). Thirdly, we tested the usefulness of mtDNA (control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Microsatellites; Mitochondrial DNA; Terns.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400029

Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum
Provedor de dados: 74
Autores: Podio,Maricel; Rodríguez,María P.; Felitti,Silvina; Stein,Juliana; Martínez,Eric J.; Siena,Lorena A.; Quarin,Camilo L.; Pessino,Silvina C.; Ortiz,Juan Pablo A.
In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen molecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence mapping...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apomixis; Chromosome walking; Gene mapping; Molecular markers.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500018

Genetic divergence in Tetragonisca angustula Latreille, 1811 (Hymenoptera, Meliponinae, Trigonini) based on rapd markers
Provedor de dados: 74
Autores: Oliveira,Rosana de Cássia; Nunes,Francis de Morais Franco; Campos,Ana Paula Soraggi; Vasconcelos,Soraya Matos de; Roubik,David; Goulart,Luiz Ricardo; Kerr,Warwick Estevam.
One of the commonest neotropical stingless bees is Tetragonisca angustula (Latreille, 1811), popularly known in Portuguese as jataí, which occurs in variable nesting sites from Mexico to Argentina. We used 18 primers to generate 218 RAPD markers which we used to determined the genetic distance between T. angustula populations from 25 localities in three different Latin America countries, using Tetragonisca weyrauchi from the Brazilian state of Acre and the common honey bee (Apis mellifera) as outgroups. Genetic distance, calculated as the Percentage of Dissimilarity (14%), based on all markers divided the T. angustula population into eastern (group 1) and western (group 2) groups. However, we were able to separate the two groups by using only two primers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tetragonisca angustula; Genetic divergence; Molecular markers; UPGMA.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200009

Reciprocal recurrent selection effects on the genetic structure of tropical maize populations assessed at microsatellite loci
Provedor de dados: 74
Autores: Pinto,Luciana Rossini; Vieira,Maria Lucia Carneiro; Souza Jr.,Claudio Lopes de; Souza,Anete Pereira de.
A modified reciprocal recurrent selection (RRS) method, which employed one cycle of high-intensity selection, was applied to two tropical maize (Zea mays L.) populations, BR-105 and BR-106, originating the improved synthetics IG-3 and IG-4, respectively. In the present study the effects of this kind of selection on the genetic structure of these populations and their synthetics were investigated at 30 microsatellite (SSR) loci. A total of 125 alleles were revealed. A reduction in the number of alleles was observed after selection, as well as changes in allele frequencies. In nearly 13% (BR-105) and 7% (BR-106) of the loci evaluated, the changes in allele frequencies were not explained, exclusively due to the effects of genetic drift. The effective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population genetics; Plant breeding; Molecular markers.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300022

Differential expression of the KLK2 and KLK3 genes in peripheral blood and tissues of patients with prostate cancer
Provedor de dados: 74
Autores: Meola,Juliana; Goulart,Luiz R.; Oliveira,Jaqueline D.D.; Neves,Adriana F.; Oliveira Jr.,Waldesse P.; Saraiva,Ana C.M.; Capaneli,Andréia C.; Cardoso,Alexandra M.; Prado,Lindolfo D.; Borba,Sebastião A.; Silva,Heyder D.
We used the multiplex semi-quantitative reverse-transcriptase PCR (RT-PCR) to investigate kallikrein 2 and 3 (KLK2 and KLK3) mRNA levels in prostate tissue from 42 prostate cancer patients, 33 of whom were also assessed for peripheral blood KLK2 expression by qualitative semi-nested RT-PCR. We found that KLK2 was an important tissue biomarker for distinguishing between prostate cancer patients and those with benign prostatic hyperplasia, particularly when KLK2 expression was &gt; 60% of that of the beta2-microglobulin constitutive gene. Patients with an average relative expression value &gt; 0.6 (cutoff value) had an eleven-fold higher chance of having prostate cancer. When one or two tissues samples were evaluated for KLK2 expression using the cutoff...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kallikrein II; Molecular markers; Prostate cancer; PSA; Semi-quantitative RT-PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200001

Association between genetic distances in wheat (Triticum aestivum L.) as estimated by AFLP and morphological markers
Provedor de dados: 74
Autores: Vieira,Eduardo Alano; Carvalho,Fernando Irajá Félix de; Bertan,Ivandro; Kopp,Mauricio Marini; Zimmer,Paulo Dejalma; Benin,Giovani; Silva,José Antônio Gonzalez da; Hartwig,Irineu; Malone,Gaspar; Oliveira,Antônio Costa de.
Molecular and morphological data analyses matrices are very informative tools for the estimation of genetic distances. We used AFLP markers, morphological traits and combined analysis to estimate the genetic distances between wheat genotypes and ascertain any associations between the two techneques. Nineteen wheat (Triticum aestivum L.) genotypes were analyzed using amplified fragment length polymorphism (AFLP) markers and field evaluated for two years. The matrices obtained by morphological and molecular marker data analyses revealed a significant but moderate correlation (r = 0.47), indicating that such techniques sample distinct genome regions. The combined analysis was found to be highly correlated with AFLP (r = 0.97) and moderately correlated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reeding; Genetic divergence; Molecular markers; Morphological traits.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300016

Genetic divergence between populations of the stingless bee uruçu amarela (Melipona rufiventris group, Hymenoptera, Meliponini): is there a new Melipona species in the Brazilian state of Minas Gerais?
Provedor de dados: 74
Autores: Tavares,Mara Garcia; Dias,Luiz Antonio dos Santos; Borges,Andréia Arantes; Lopes,Denilce Meneses; Busse,Angélica Helene Pereira; Costa,Ronaldo Guimarães; Salomão,Tânia Maria Fernandes; Campos,Lucio Antonio de Oliveira.
Allozyme, microsatellite and random amplified polymorphic DNA (RAPD) molecular markers were used to investigate the within and between population genetic variability and between population genetic differentiation of the Brazilian stingless bee uruçu amarela (nominally Melipona rufiventris Lepeletier, 1836) present in savanna and Atlantic forest habitats of the Brazilian state of Minas Gerais (MG). We found low levels of within population variability, although there were a large number of private alleles that specifically characterized these populations. The F ST values indicated a high level of genetic diversity between populations. Analysis of molecular variance (AMOVA) showed a high degree of population differentiation between the savanna and Atlantic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endangered species; Genetic divergence; Molecular markers; Stingless bees; Uruçu amarela.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400027

Genetic diversity of Bemisia tabaci (Genn.) Populations in Brazil revealed by RAPD markers
Provedor de dados: 74
Autores: Lima,L.H.C.; Campos,L.; Moretzsohn,M.C.; Návia,D.; Oliveira,M.R.V. de.
Bemisia tabaci (Genn.) was considered a secondary pest in Brazil until 1990, despite being an efficient geminivirus vector in beans and soybean. In 1991, a new biotype, known as B. tabaci B biotype (=B. argentifolii) was detected attacking weed plants and causing phytotoxic problems in Cucurbitaceae. Nowadays, B. tabaci is considered one of the most damaging whitefly pests in agricultural systems worldwide that transmits more than 60 different plant viruses. Little is known about the genetic variability of these populations in Brazil. Knowledge of the genetic variation within whitefly populations is necessary for their efficient control and management. The objectives of the present study were to use RAPD markers (1) to estimate the genetic diversity of B....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Whitefly; Molecular markers; UPGMA; AMOVA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200016

Genetic structure of Argentinean hexaploid wheat germplasm
Provedor de dados: 74
Autores: Vanzetti,Leonardo S.; Yerkovich,Nadia; Chialvo,Eugenia; Lombardo,Lucio; Vaschetto,Luis; Helguera,Marcelo.
The identification of genetically homogeneous groups of individuals is an ancient issue in population genetics and in the case of crops like wheat, it can be valuable information for breeding programs, genetic mapping and germplasm resources. In this work we determined the genetic structure of a set of 102 Argentinean bread wheat (Triticum aestivum L.) elite cultivars using 38 biochemical and molecular markers (functional, closely linked to genes and neutral ones) distributed throughout 18 wheat chromosomes. Genetic relationships among these lines were examined using model-based clustering methods. In the analysis three subpopulations were identified which correspond largely to the origin of the germplasm used by the main breeding programs in Argentina.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; Genetic structure; Molecular markers; Genetic diversity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300014

Taxonomic and evolutionary analysis of Zaprionus indianus and its colonization of Palearctic and Neotropical regions
Provedor de dados: 74
Autores: Commar,Leliane Silva; Galego,Luis Gustavo da Conceição; Ceron,Carlos Roberto; Carareto,Claudia Marcia Aparecida.
Zaprionus indianus is a dipteran (Drosophilidae) with a wide distribution throughout the tropics and temperate Palearctic and Nearctic regions. There have been proposals to reclassify the genus Zaprionus as a subgenus or group of the genus Drosophila because various molecular markers have indicated a close relationship between Zaprionus species and the immigrans-Hirtodrosophila radiation within Drosophila. These markers, together with alloenzymes and quantitative traits, have been used to describe the probable scenario for the expansion of Zaprionus indianus from its center of dispersal (Africa) to regions of Asia (ancient dispersal) and the Americas (recent dispersal). The introduction of Z. indianus into Brazil was first reported in 1999 and the current...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alloenzyme; Bioinvasion; Molecular markers; Phylogenetic analysis; Quantitative traits.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300003

Genetic similarity among soybean (Glycine max (L) Merrill) cultivars released in Brazil using AFLP markers
Provedor de dados: 74
Autores: Bonato,Ana Lídia V.; Calvo,Eberson S.; Geraldi,Isaias O.; Arias,Carlos Alberto A.
Genetic similarity among soybean genotypes was studied by applying the amplified fragment length polymorphism (AFLP) technique to 317 soybean cultivars released in Brazil from 1962 to 1998. Genetic similarity (GS) coefficients were estimated using the coefficient of Nei and Li (Nei and Li 1979), and the cultivars were clustered using the unweighted pair-group method with averages (UPGMA). The parentage coefficients of 100 cultivars released between 1984 and 1998 were calculated and correlated with the genetic similarity obtained by the markers. The genetic similarity coefficients varied from 0.17 to 0.97 (x = 0.61), with 56.8% of the coefficients being above 0.60 and only 9.7% equal to or less than 0.50. The similarity coefficients have remained constant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Genetic similarity; Molecular markers; Parentage coefficient; Soybean.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400019

Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrot
Provedor de dados: 74
Autores: Santos,Carlos A.F.; Simon,Philipp W.
Amplified fragment length polymorphism (AFLP) is a fast and reliable tool to generate a large number of DNA markers. In two unrelated F2 populations of carrot (Daucus carota L.), Brasilia x HCM and B493 x QAL (wild carrot), it was hypothesized that DNA 1) digested with the same restriction endonuclease enzymes and amplified with the same primer combination and 2) sharing the same position in polyacrylamide gels should be conserved sequences. To test this hypothesis AFLP fragments from polyacrylamide gels were eluted, reamplified, separated in agarose gels, purified, cloned and sequenced. Among thirty-one paired fragments from each F2 population, twenty-six had identity greater than 91% and five presented identity of 24% to 44%. Among the twenty-six...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Daucus carota; Evolution; Molecular markers; SCAR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200013

Developing a common bean core collection suitable for association mapping studies
Provedor de dados: 74
Autores: Perseguini,Juliana Morini Küpper Cardoso; Silva,Gliciane Micaele Borges; Rosa,João Ricardo Bachega Feijó; Gazaffi,Rodrigo; Marçal,Jéssica Fernanda; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando; Zucchi,Maria Imaculada; Garcia,Antonio Augusto Franco; Benchimol-Reis,Luciana Lasry.
Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Genetic diversity; Genetic structure; Microsatellites; Phaseolus vulgaris L.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100067

Quantitative trait loci (QTL) mapping for growth traits on bovine chromosome 14
Provedor de dados: 74
Autores: Miyata,Marcelo; Gasparin,Gustavo; Coutinho,Luiz Lehmann; Martinez,Mario Luiz; Machado,Marco Antonio; Silva,Marcos Vinicius G. Barbosa da; Campos,Ana Lucia; Sonstegard,Tad S.; Rosário,Millor Fernandes do; Regitano,Luciana Correia de Almeida.
Quantitative trait loci (QTL) mapping in livestock allows the identification of genes that determine the genetic variation affecting traits of economic interest. We analyzed the birth weight and weight at 60 days QTL segregating on bovine chromosome BTA14 in a F2 resource population using genotypes produced from seven microsatellite markers. Phenotypes were derived from 346 F2 progeny produced from crossing Bos indicus Gyr x Holstein Bos taurus F1 parents. Interval analysis to detect QTL for birth weight revealed the presence of a QTL (p &lt; 0.05) at 1 centimorgan (cM) from the centromere with an additive effect of 1.210 ± 0.438 kg. Interval analysis for weight at 60 days revealed the presence of a QTL (p &lt; 0.05) at 0 cM from the centromere with an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TA14; Cattle; F2; Growth traits; Molecular markers; QTL.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300011

Polymorphic heterologous microsatellite loci for population genetics studies of the white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae)
Provedor de dados: 74
Autores: Souza,Andiara Silos Moraes de Castro e; Miño,Carolina Isabel; Lama,Silvia Nassif Del.
We screened 44 heterologous microsatellites isolated in species of the families Threskiornithidae, Ciconiidae and Ardeidae for their use in a migratory waterbird, the white-faced ibis Plegadis chihi (Vieillot, 1817) (Threskiornithidae). Of the screened loci, 57% amplified successfully and 24% were polymorphic. In two breeding colonies from southern Brazil (N = 131) we detected 32 alleles (2-10 alleles/ locus). Average He over all loci and colonies was 0.55, and the combined probability of excluding false parents, 98%. There was no departure from HWE in any loci or population. Eru6 and Eru4 loci were in non-random association in the Alvorada colony, and NnNF5 and Eru5 in both populations. AMOVA analysis indicated that most of the genetic diversity was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cross-species amplification; Genetic diversity; Molecular markers; White-faced ibis; SSR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100010

Predicting performance of soybean populations using genetic distances estimated with RAPD markers
Provedor de dados: 74
Autores: Barroso,Paulo Augusto Vianna; Geraldi,Isaias Olívio; Vieira,Maria Lúcia Carneiro; Pulcinelli,Carlos Eduardo; Vencovsky,Roland; Dias,Carlos Tadeu dos Santos.
In order to verify whether genetic distance (GD) is associated with population mean (PM), genetic variance (GV) and the proportion of superior progenies generated by each cross in advanced generations of selfing (PS), the genetic distances between eight soybean lines (five adapted and three non-adapted) were estimated using 213 polymorphic RAPD markers. The genetic distances were partitioned according to Griffing's Model I Method 4 for diallel analysis, i.e., GDij = GD+ GGDi+ GGDj + SGDij. Phenotypic data were recorded for seed yield and plant height for 25 out of 28 populations of a diallel set derived from the eight soybean lines and evaluated from F2:8 to F2:11 generations. No significant correlation for seed yield was detected between GD and GV, while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean; Genetic distance; Molecular markers; RAPD; Prediction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300020

Population structures of Brazilian Tall coconut (Cocos nucifera L.) by microsatellite markers
Provedor de dados: 74
Autores: Ribeiro,Francisco E.; Baudouin,Luc; Lebrun,Patricia; Chaves,Lázaro J.; Brondani,Claudio; Zucchi,Maria I.; Vencovsky,Roland.
Coconut palms of the Tall group were introduced to Brazil from the Cape Verde Islands in 1553. The present study sought to evaluate the genetic diversity among and within Brazilian Tall coconut populations. Samples were collected of 195 trees from 10 populations. Genetic diversity was accessed by investigating 13 simple sequence repeats (SSR) loci. This provided a total of 68 alleles, ranging from 2 to 13 alleles per locus, with an average of 5.23. The mean values of gene diversity (He) and observed heterozygosity (Ho) were 0.459 and 0.443, respectively. The genetic differentiation among populations was estimated at θ = 0.1600 and the estimated apparent outcrossing rate was t a = 0.92. Estimates of genetic distances between the populations varied from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coconut; Genetic diversity; Genetic variability; Molecular markers; SSR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400016

Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves)
Provedor de dados: 74
Autores: Faria,Patrícia J.; Miyaki,Cristina Y.
The selection of molecular markers for population studies is an important tool for biodiversity conservation. The family Psittacidae contains many endangered and vulnerable species and we tested three kinds of molecular markers for their potential use in population studies of five psitacid species: 43 hyacinth macaws (Anodorhynchus hyacinthinus), 42 blue-and-yellow macaws (Ara ararauna), 23 red-and-green macaws (Ara chloroptera), 19 red-spectacled amazons (Amazona pretrei); and 18 red-tailed amazons (Amazona brasiliensis). We tested 21 clones from a genomic library of golden conure (Guarouba guarouba) minisatellites and 12 pairs of microsatellite primers developed for the domestic chicken (Gallus gallus) and A. hyacinthinus. We also tested seven...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Minisatellites; Molecular markers; Parrots; Population studies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200007

Genetic diversity of carotenoid-rich bananas evaluated by Diversity Arrays Technology (DArT)
Provedor de dados: 74
Autores: Amorim,Edson P.; Vilarinhos,Alberto D.; Cohen,Kelly O.; Amorim,Vanusia B.O.; Santos-Serejo,Janay A. dos; Silva,Sebastião Oliveira e; Pestana,Kátia N.; Santos,Vânia J. dos; Paes,Norma S.; Monte,Damares C.; Reis,Ronaldo V. dos.
The aim of this work was to evaluate the carotenoid content and genetic variability of banana accessions from the Musa germplasm collection held at Embrapa Cassava and Tropical Fruits, Brazil. Forty-two samples were analyzed, including 21 diploids, 19 triploids and two tetraploids. The carotenoid content was analyzed spectrophotometrically and genetic variability was estimated using 653 DArT markers. The average carotenoid content was 4.73 µg.g-1, and ranged from 1.06 µg.g-1 for the triploid Nanica (Cavendish group) to 19.24 µg.g-1 for the triploid Saney. The diploids Modok Gier and NBA-14 and the triploid Saney had a carotenoid content that was, respectively, 7-fold, 6-fold and 9-fold greater than that of cultivars from the Cavendish group (2.19 µg.g-1)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diploids; Germplasm; Molecular markers; Musa sp.; Variability.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100014

Genetic diversity of tropical-adapted onion germplasm assessed by RAPD markers
Provedor de dados: 75
Autores: Santos,Maria do Desterro M dos; Ragassi,Carlos Francisco; Fonseca,Maria Esther de N; Buzar,Anne Gizelle R; Oliveira,Valter R; Melo,Paulo César T de; Boiteux,Leonardo S.
Onion is a crop of significant socioeconomic importance to Brazil. Onion germplasm with adaptation to tropical and sub-tropical conditions has played an important role in the development of this crop in the country. In this context, we studied the genetic diversity in a germplasm collection potentially useful for the development of cultivars for tropical and subtropical regions. The genetic variability of 21 accessions/cultivars that have been used as germplasm and/or were developed by onion breeding programs in Brazil was evaluated via RAPD markers. The following accessions were included in the study :'Red Creole', 'Roxa IPA-3', 'Valenciana 14', 'Beta Cristal', 'Diamante', 'Composto IPA-6', 'Aurora', 'Bojuda Rio Grande', 'Alfa Tropical', 'Pêra IPA-4',...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Molecular markers; Plant breeding.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000100019

Yam: a neglected and underutilized crop in Brazil
Provedor de dados: 75
Autores: Siqueira,MVBM.
In Brazil current studies and investments on yams are incipient. Similarly, the literature in recent decades lacks adequate information on this group of plants. The existing literature, on its turn, requires more than ever to be revised and organized. Yams have joined the so-called "neglected" group of crops for several reasons, but particularly because they are associated with poor and traditional communities. Many vegetables introduced in Brazil during the colonization period have adapted to different cropping systems, yams being an excellent example. This diversity resulted very widespread, yet poorly recognized in the country. In turn, the gardens using traditional farming systems continue to maintain and enhance yam local varieties. Studies from other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dioscorea spp.; Tubers; Traditional agriculture; Agrobiodiversity; Molecular markers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000100003

Genetic diversity among natural populations of Mandevilla velutina
Provedor de dados: 75
Autores: Bertoni,Bianca Waléria; Souza,Ana Valéria de; Biondo,Ronaldo; França,Suzelei de C; Telles,Mariana PC; Pereira,Ana Maria S.
Mandevilla velutina (Mart. Ex Stadelm) Woodson (Apocynaceae) is an endemic species in the Cerrado (Brazilian Savannah), whose alcoholic extracts and root infusions are extensively used in the popular medicine to treat inflammatory diseases and against snake bites. Currently, this species has been pointed out as crucial in conservation programs. Therefore, studies on its genetic diversity, as well as the development of methodology for in vitro conservation in germplasm banks are imperative to avoid its extinction. The objective of this work was to investigate the intra- and inter-populational genetic variability of M. velutina to enhance the representativeness of germplasm banks. To this aim, we used RAPD molecular markers. The analysis of molecular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mandevilla velutina; Medicinal plant; Extinction risk; Molecular markers.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362010000200012

Comparison of chili pepper breeding populations for agronomic traits and polygenic resistance to Phytophthora blight
Provedor de dados: 75
Autores: Arpaci,Bekir Bülent; Karataş,Kerim.
ABSTRACT Belonging to the Oomycete class, Phytophthora capsici has wide range of host profile and is responsible for many devastating diseases in many countries. In addition to time consuming problem for transferring resistance to susceptible varieties, backcrossing method causes losing of genes providing resistance to susceptible varieties. In this study transferring P. capsici resistance genes to susceptible chili pepper lines was aimed during the extensive breeding period and resistant lines were confirmed by marker assistance. Two different breeding populations from CM334 and PM217 were compared by stem inoculation test to determine receptivity, inducibility, and stability resistance component. CM334 was found more effective for transferring all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum annuum; Phytophthora capsici; Resistance component; Molecular markers; Crop breeding.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000100012

Caracterização de germoplasma e melhoramento genético do maracujazeiro assistidos por marcadores moleculares - fase 2: resultados de pesquisa 2008-2012.
Provedor de dados: 14
Autores: FALEIRO, F. G.; JUNQUEIRA, N. T. V.; OLIVEIRA, E. J. de; MACHADO, C. de F.; PEIXOTO, J. R.; COSTA, A. M.; GUIMARAES, T. G.; JUNQUEIRA, K. P.
bitstream/item/128192/1/doc-324.pdf
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Lançamento de variedade; Passion fruit; Molecular markers; Cultivar evaluation.; Maracujá; Passiflora edulis; Recurso Genético; Transferência de Tecnologia.; Plant breeding.
Ano: 2014
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1019176

Extração de DNA genômico de Brachiaria e Panicum maximum.
Provedor de dados: 14
Autores: BONATO, A. L. V.; VALLE, C. B. do; JANK, L.; RESENDE, R. M. S.; LEGUIZAMON, G. O. de C.
bitstream/CNPGC/10572/1/COT79.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Molecular markers; Processing.; Brachiaria; DNA; Marcador Molecular; Melhoramento Genético Vegetal; Panicum Maximum; Processamento.; Plant breeding.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/319897

Extração de DNA genômico de Stylosanthes spp.
Provedor de dados: 14
Autores: BONATO, A. L. V.; VERZIGNASSI, J. R.; RESENDE, R. M. S.; FERNANDES, C. D.; LEGUIZAMON, G. O. de C.
Protocolo. Considerações sobre os procedimentos.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Molecular markers; Processing.; DNA; Marcador Molecular; Melhoramento Genético Vegetal; Processamento.; Stylosanthes; Plant breeding.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/318167

De novo assembly, characterization and functional annotation of Senegalese sole (Solea senegalensis) and common sole (Solea solea) transcriptomes: integration in a database and design of a microarray
Provedor de dados: 5
Autores: Benzekri, Hicham; Armesto, Paula; Cousin, Xavier; Rovira, Mireia; Crespo, Diego; Alejandro Merlo, Manuel; Mazurais, David; Bautista, Rocio; Guerrero-fernandez, Dario; Fernandez-pozo, Noe; Ponce, Marian; Infante, Carlos; Zambonino, Jose Luis; Nidelet, Sabine; Gut, Marta; Rebordinos, Laureana; Planas, Josep V.; Begout, Marie-laure; Gonzalo Claros, M.; Manchado, Manuel.
Background Senegalese sole (Solea senegalensis) and common sole (S. solea) are two economically and evolutionary important flatfish species both in fisheries and aquaculture. Although some genomic resources and tools were recently described in these species, further sequencing efforts are required to establish a complete transcriptome, and to identify new molecular markers. Moreover, the comparative analysis of transcriptomes will be useful to understand flatfish evolution. Results A comprehensive characterization of the transcriptome for each species was carried out using a large set of Illumina data (more than 1,800 millions reads for each sole species) and 454 reads (more than 5 millions reads only in S. senegalensis), providing coverages ranging from...
Tipo: Text
Palavras-chave: Soles; Transcriptome; Microarray; Orthology; Molecular markers; SoleaDB.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00238/34918/33626.pdf

Recent invasion of the Japanese oyster drill along the French Atlantic coast: identification of specific molecular markers that differentiate Japanese, Ocinebrellus inornatus, and European, Ocenebra erinacea, oyster drills
Provedor de dados: 5
Autores: Garcia Meunier, Pascale; Martel, Corine; Pigeot, Jacques; Chevalier, Guillemette; Blanchard, Gérard; Goulletquer, Philippe; Robert, Stephane; Sauriau, Pierre-guy.
The direct amplification of length polymorphism technique (DALP) has been used to distinguish species-specific banding patterns in two marine gastropod oyster drills Ocenebra erinacea (Linnaeus, 1758) and Ocinebrellus inornatus (Recluz, 1851). Ocenebra erinacea is the European oyster drill, common along all European coasts. Ocinebrellus inornatus, the Japanese oyster drill, was recorded in oyster growing areas of the Marennes-Oleron Bay (SW France) for the first time in 1995. This new biological invasion could lead to an increase, which must be evaluated, in the predation risk for cultivated species i.e. oysters and blue mussels, and for littoral fishing resources along the French Atlantic coasts. As a result, since specific identification of early life...
Tipo: Text
Palavras-chave: French Atlantic coast; Ocenebra erinacea; Ocinebrellus inornatus; Marine bioinvasions; Molecular markers.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-569.pdf

Molecular markers as a tool to study genetic resources in oysters
Provedor de dados: 5
Autores: Boudry, Pierre.
Despite the world economic importance of oyster farming, genetic improvement has not yet had a great impact on these species. Oyster farming is traditionnally based on wild stocks, whose natural populations are often overexploited, and frequently does not fulfil market demande....
Tipo: Text
Palavras-chave: Molecular markers; Genetic resources; Oysters.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/acte-3212.pdf

Molecular and morphological data from Thoosidae in favour of the creation of a new suborder of Tetractinellida
Provedor de dados: 5
Autores: Luis Carballo, Jose; Bautista-guerrero, Eric; Cardenas, Paco; Cruz-barraza, Jose Antonio; Aguilar-camacho, Jose Maria.
The Thoosidae (Porifera, Demospongiae, Tetractinellida) currently includes the genera Thoosa, Alectona, and Delectona. To this date, molecular data are only available for Alectona. In this study, the phylogenetic affinities of the genera Thoosa and Alectona have been investigated with the species T. mismalolli, T. calpulli, and T. purpurea from the Mexican Pacific using morphology and three molecular loci: the mitochondrial cytochrome oxidase subunit 1 (CO1 mtDNA), 28S rRNA (fragment D2), and 18S rRNA. Morphology and embryology showed that these genera are quite different from the rest of the tetractinellids because larvae of Alectona and Thoosa have unique features in sponges, such as the presence of monaxonic discs in Thoosa and tetraxonic discs in...
Tipo: Text
Palavras-chave: Astrophorina; Molecular markers; Porifera; Systematics; Synapomorphy; Thoosidae.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00688/79996/83503.pdf

Diversité et différentiation génétique chez l'huître plate européenne : de la population à l'individu (Actes)
Provedor de dados: 5
Autores: Boudry, Pierre; Launey, Sophie; Diaz-almela, A.; Naciri-graven, Yamama; Ledu, Christophe; Mira, S.; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie.
PCR-based DNA markers enable the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European fiat oyster (Ostrea edulis) populations aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Similar patterns of variation were found using allozymes, microsatellites and mitochondrial DNA, aIl which support an isolation by distance model. High variability was observed in the range of diversity between populations using a mitochondrial marker, a reflection of small effective population sizes in sorne locations. A 10fold quantitative difference was observed in Fst,...
Tipo: Text
Palavras-chave: Recruitment; Diversity; Molecular markers; Population genetics; Ostrea edulis; Flat oyster; Dynamique de recrutement; Diversité génétique; Microsatellites; Marqueurs moléculaires; Génétique des populations; Ostrea edulis; Huître plate.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-2851.pdf

Bovine &amp;#956;-calpain (CAPN1) gene polymorphisms in Brangus and Brahman bulls
Provedor de dados: 46
Autores: Soria,LA; Corva,PM; Huguet,MJ; Miño,S; Miquel,MC.
The bovine CAPN1 gene encodes the large subunit of &amp;#956;-calpain, which is thought to be one of the most important enzymes involved in postmortem beef tenderization. Three SNPs in CAPN1 (SNP 316, SNP 530 and SNP 4751) have been associated with beef tenderness in different beef cattle breeds. The objective of this work was to implement genotyping strategies for CAPN1 markers as part of a project pursuing the identifi cation and validation of molecular markers associated with bovine meat quality and composition. Three PCR-RFLP methods were designed to determine genotypes of 64 bulls (11 Angus, 43 Brangus and 10 Brahman). Unexpected patterns resulting from the PCR-RFLP analysis at SNP 316 and SNP 530 were resolved by cloning and sequencing and lead to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Meat tenderness; U-calpain; Molecular markers; Single nucleotide polymorphism.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332010000100007

Terrestrial planarians (Platyhelminthes, Tricladida, Terricola) from the Iberian peninsula: first records of the family Rhynchodemidae, with the description of a new Microplana species
Provedor de dados: 16
Autores: Mateos, Eduardo; Giribet, Gonzalo; Carranza, Salvador.
The first records of terrestrial planarians belonging to the family Rhynchodemidae are reported for the Iberian Peninsula. A new endemic species from the Spanish Pyrenees, Microplana nana sp. nov., is described. The characteristic features of this species are: i) small size (8-10 mm) in adult individuals, ii) very long conical penis papilla and iii) absence of seminal vesicle, bursa copulatrix, genito-intestinal duct, and well-developed penial bulb. Moreover, the widespread common European land planarian Microplana terrestris (Müller, 1774) is reported for the first time from the Iberian Peninsula. The two species, M. nana sp. nov. and M. terrestris, are described by means of external morphology using histological sections, and have been characterized by...
Tipo: Article / Letter to the editor
Palavras-chave: New species; ITS-1; Molecular markers; Iberian peninsula; Microplana; Rhynchodemidae; Terricola.
Ano: 1998
URL: http://www.repository.naturalis.nl/record/534286

Estrutura genética populacional de Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) utilizando marcadores microssatélites
Provedor de dados: 82
Autores: Valle,GE do; Zucchi,MI; Stabellini,NS; Lourenção,AL; Pinheiro,JB.
We aimed to characterize the population genetic structure within and among five Bemisia tabaci (Gennadius) populations collected from different host plants and geographic regions by using microssatelites as a molecular marker. Each population was represented by 19 specimens. The host plants and geographic origins of these populations were described as follows: Pop 1: Squash Barreiras (BA); Pop 2: Cotton Barreiras (BA); Pop 3: Soybean Campinas (SP); Pop 4: Tomato Cruz das Almas (BA); and Pop 5: Soybean Rondonópolis (MT). Six polymorphic loci were observed, which discriminated 31 different alleles in the studied populations, with a mean number of alleles per population of 3.30 (2.67 - 4.00). Using Fisher's Exact test, it was observed that at least three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Molecular markers; Whitefly.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000200008

Molecular identification of intergenus crosses involving catfish hybrids: risks for aquaculture production
Provedor de dados: 83
Autores: Hashimoto,Diogo T.; Prado,Fernanda D.; Foresti,Fausto; Porto-Foresti,Fábio.
ABSTRACT Monitoring of the interspecific hybrid production and trade is essential for the appropriate management of these animals in fish farms. The identification of catfish hybrids by morphological analysis is unreliable, particularly of juveniles and post-F1 individuals. Therefore, in the present study, we used five molecular markers (four nuclear genes and one mitochondrial gene) to detect hybrids in the trade of pimelodid juvenile fish from different stocks purchased of five seed producers in Brazil. Samples commercialized as pintado (pure species Pseudoplatystoma corruscans ) from three fish farms were genetically identified as hybrid cachapinta (♀ P. reticulatum x ♂ P. corruscans ). In the stocks purchased as cachandiá (hybrid between ♀ P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish farm; Hybridization; Molecular markers; Nuclear genes; Pimelodidae.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200213

Development and characterization of fifteen polymorphic microsatellite loci in Bryconamericus aff. iheringii (Teleostei: Characidae) and cross-amplification in related Characidae species
Provedor de dados: 83
Autores: Apolinário-Silva,Caroline; Ferreira,Dhiego G.; Cavenagh,Analiza F.; Aprígio,Nícollas G. O.; Galindo,Bruno A.; Carlsson,Jens; Sofia,Silvia H.
ABSTRACT Data on 15 novel microsatellite loci from the Neotropical fish Bryconamericus aff. iheringii are presented here. Analyses of 32 individuals from four different streams revealed 192 different alleles, ranging from four to 32 alleles per locus (mean of 12.8 per locus). Observed and expected heterozygosities ranged from 0.094 to 0.813 and 0.205 to 0.952, respectively. These loci showed high polymorphic information content and will be a resource for genetic studies of B. aff. iheringii. Furthermore, several loci also amplified other small Neotropical Characidae (Piabarchus stramineus and Piabina argentea) and should be useful for these species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Lambarizinho; Molecular markers; Neotropical fish; SSR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000100218

Microsatellite loci development for three catfish species from northwestern South America
Provedor de dados: 83
Autores: Restrepo-Escobar,Natalia; Márquez,Edna J.
ABSTRACT The Neotropical catfish species Ageneiosus pardalis, Pimelodus grosskopfii, and Sorubim cuspicaudus are important fishery resources in Colombia that show historical declines in their capture. This study used next-generation sequencing with 454 FLX technology (Roche Applied Science) and bioinformatics analysis to develop between 18 and 24 microsatellite loci for these species. The novel microsatellite loci showed high values of polymorphic information content -PIC (A. pardalis: 0.601-0.903, P. grosskopfii: 0.748-0.946 and S. cuspicaudus: 0.383-0.876), and the average number of alleles/locus ranged from 7-15 for A. pardalis, 9-30 for P. grosskopfii and 5-14 for S. cuspicaudus. The average observed and expected heterozygosities were respectively,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater fish; Molecular markers; Next-generation sequencing; Siluriformes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000100210

Genetic diversity and population structure of Brycon nattereri (Characiformes: Bryconidae): a Neotropical fish under threat of extinction
Provedor de dados: 83
Autores: Galindo,Bruno A.; Ferreira,Dhiego G.; Apolinário-Silva,Caroline; Terra,Mariana C.; Aprígio,Nícollas G. O.; Ota,Renata R.; Ohara,Willian M.; Almeida,Fernanda S.; Sofia,Silvia H.
ABSTRACT Brycon nattereri is an endangered Neotropical fish reported along conserved stretches of the upper Paraná, Tocantins and São Francisco rivers. Populations of this species have been very rare in some Paraná River sub basins. This study analyzes the genetic diversity and population structure of B. nattereri in a restricted area of occurrence recently identified in upper Paraná River basin. Seven microsatellite loci and 497 bp of D-Loop mitochondrial region were examined in 92 individuals from four points along the area of occurrence. Both molecular markers indicated a single population distributed along a stretch of the river approximately 80 km long. Although some of the data suggest an ancient bottleneck, current levels of genetic diversity (H  E...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Endangered species; Molecular markers; Population genetics; Pirapitinga.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000100208

Genetic identification of interspecific hybrid of Neotropical catfish species (Pseudoplatystoma corruscans vs. Pseudoplatystoma reticulatum) in rivers of Mato Grosso do Sul State, Brazil
Provedor de dados: 83
Autores: Vaini,Jussara Oliveira; Grisolia,Alexéia Barufatti; Prado,Fernanda Dotti do; Porto-Foresti,Fábio.
Hybrids of the species Pseudoplatystoma corruscansvs.Pseudoplatystoma reticulatum are extensively traded because they are more docile and precocious compared to their parents. This study aimed to identify the occurrence of hybrid 'Surubim' in rivers of the State of Mato Grosso do Sul, applying molecular techniques. Pseudoplatystoma corruscans, Pseudoplatystoma reticulatum and hybrid 'Surubim' were detected using multiplex PCR and PCR-RFLP in RAG2, GLOBIN, EF1α, 18S rRNA nuclear genes, and mitochondrial 16S rRNA gene. The results revealed the presence of F1 hybrids in rivers and the occurrence of genetic introgression, evidenced by the Post-F1 hybrids. Furthermore, discrepancies between the morphological and genetic identification were observed,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Fish; Hybridization; Introgression; Molecular markers.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000300635

Genetic diversity of Brazilian Pantaneiro horse and relationships among horse breeds
Provedor de dados: 86
Autores: Sereno,Fabiana Tavares Pires de Souza; Sereno,José Robson Bezerra; Vega-Pla,Jose Luis; Kelly,Lucy; Bermejo,Juan Vicente Delgado.
The objective of this work was to evaluate the genetic diversity of Brazilian Pantaneiro horse by microsatellite markers, investigate the effect of genetic bottlenecks and estimate genetic differentiation among four horse breeds. Genetic variation was estimated through allele frequencies and mean breed heterozygosity. Nei's genetic distances among the breeds Pantaneiro, Thoroughbred, Arabian, Spanish Pure Breed (Andalusian), and Uruguay Creole were calculated, and it was used to construct an UPGMA dendrogram. Clustering at different K values was calculated to infer population structure and assign individuals to populations. Nei's distances showed a minimum distance between Pantaneiro horse and Spanish Pure Breed (0.228), and similar distances from Spanish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equus caballus; Animal conservation; Molecular markers; Microsatellites; Genetic resources conservation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500007

QTLs for resistance to soybean cyst nematode, races 3, 9, and 14 in cultivar Hartwig
Provedor de dados: 86
Autores: Ferreira,Marcia Flores da Silva; Cervigni,Gerardo Domingo Lucio; Ferreira,Adésio; Schuster,Ivan; Santana,Fernanda Abreu; Pereira,Waldir Dias; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves.
The objective of this work was to identify major and minor-effect quantitative trait loci (QTL) for resistance to races 3, 9, and 14 of soybean cyst nematode (SCN) in Hartwig cultivar; to map new resistance QTLs for these races; and to check for the existence of epistatic interactions between QTLs. Cultivar Hartwig is an important resistance source to SCN. Recombinant inbred lines (RIL) obtained from a cross between 'Hartwig' (resistant) and Y23 (susceptible) were evaluated regarding resistance to the three races. New genomic regions for resistance to SCN were identified by microsatellites. Four QTLs, which explained between 12 and 34% of phenotypic variance, were detected for resistance to race 3 in linkage groups (LG) A2, G, J, and M. The QTL in LG G is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Breeding; Marker-assisted selection; Molecular markers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000400012

Genetic distance estimated by RAPD markers and its relationship with hybrid performance in maize
Provedor de dados: 86
Autores: Bruel,Daniela Cristina; Carpentieri-Pípolo,Valéria; Gerage,Antonio Carlos; Fonseca Júnior,Nelson da Silva; Prete,Cássio Egídio Cavenaghi; Ruas,Claudete de Fátima; Ruas,Paulo Maurício; Souza,Silvia Graciele Hülse de; Garbuglio,Deoclécio Domingos.
The objective of this work was to evaluate the genetic diversity of 16 maize inbred lines, and to determine the correlation between genetic distance and hybrid performance, using random amplified polymorphic DNA (RAPD) molecular markers. Twenty-two different random primers were used, which resulted in the amplification of 265 fragments, 237 (84.44%) of them being polymorphic. A genetic similarity matrix was created from the RAPD data, using Jaccard coefficient, and a dendrogram was constructed. Hybrid analyses were carried out using random block design and Griffing method VI for diallel crossings. The genetic associations showed five distinct heterotic groups. Correlations between genetic divergences detected by RAPD, as well as the means observed in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Diallel; Heterotic patterns; Molecular markers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006001000006

Quantification of the diversity among common bean accessions using Ward-MLM strategy
Provedor de dados: 86
Autores: Cabral,Pablo Diego Silva; Soares,Taís Cristina Bastos; Gonçalves,Leandro Simões Azeredo; Amaral Júnior,Antonio Teixeira do; Lima,Andreia Barcelos Passos; Rodrigues,Rosana; Matta,Frederico de Pina.
The present work aimed at evaluating the divergence among common bean accessions by their agronomic, morphological and molecular traits, based on the Ward-MLM procedure. A collection of 57 accessions from the gene bank of Universidade Federal do Espírito Santo was used in this study, from which: 31 were landraces belonging to the community Fortaleza, in the municipality of Muqui, ES, Brazil; 20 accessions were provided by Embrapa Trigo; and 6 were commercial cultivars. Five agronomic traits (plant cycle, number of seeds per pod, number of pods per plant, weight of 100 seeds, and grain yield), five morphological traits (growth habit, plant size, seed shape, seed color, and commercial group) and 16 microsatellite primers were evaluated. High genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Genetic diversity; Germplasm characterization; Joint analysis; Molecular markers; Morphological markers.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001000011

Genetic variability of rice recurrent selection populations as affected by male sterility or manual recombination
Provedor de dados: 86
Autores: Pinheiro,Letícia da Silveira; Rangel,Paulo Hideo Nakano; Vianello,Rosana Pereira; Brondani,Claudio.
The objective of this work was to determine the effect of male sterility or manual recombination on genetic variability of rice recurrent selection populations. The populations CNA-IRAT 4, with a gene for male sterility, and CNA 12, which was manually recombined, were evaluated. Genetic variability among selection cycles was estimated using14 simple sequence repeat (SSR) markers. A total of 926 plants were analyzed, including ten genitors and 180 individuals from each of the evaluated cycles (1, 2 and 5) of the population CNA-IRAT 4, and 16 genitors and 180 individuals from each of the cycles (1 and 2) of CNA 12. The analysis allowed the identification of alleles not present among the genitors for both populations, in all cycles, especially for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa; Allele variation; Rice breeding; Molecular markers; Population improvement.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000600011

Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers
Provedor de dados: 86
Autores: Oliveira,Roberto Pedroso de; Cristofani,Mariângela; Machado,Marcos Antônio.
The objective of this work was to construct linkage maps of 'Pêra' sweet orange [Citrus sinensis (L.) Osbeck] and 'Cravo' mandarin (Citrus reticulata Blanco) using RAPD markers and the pseudo-testcross strategy. The parents were chosen according to the resistance/susceptibility to citrus variegate chlorosis (CVC). The segregation of 176 markers was analyzed in 94 progeny of F1 hybrids, which were obtained from controlled crossings. The linkage map of 'Pêra' sweet orange had 117 markers defined by 12 linkage groups, which spanned 612.1 cM. Only six markers could not be linked to the linkage group and 48.7% of the markers showed segregation distortion. The linkage map of 'Cravo' mandarin had 51 markers defined by 12 linkage groups, which spanned 353.3 cM....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Dominant markers; Molecular markers; Segregation; Breeding methods.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000200009

Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance
Provedor de dados: 86
Autores: Pinto,Fernanda de Oliveira; Maluf,Mirian Perez; Guerreiro-Filho,Oliveiro.
The objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'-UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leucoptera coffeella; Microsatellites; Molecular markers.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000300011

Morphoagronomic and molecular characterization of ornamental passion fruit cultivars
Provedor de dados: 86
Autores: Fonseca,Kenia Gracielle da; Faleiro,Fábio Gelape; Junqueira,Nilton Tadeu Vilela; Barth,Mariana; Feldberg,Nelson Pires.
Abstract: The objective of this work was to validate the morphoagronomic descriptors used in the protection processes of plant cultivars in Brazil, by characterizing six cultivars of ornamental passion fruit. The BRS Rubiflora, BRS Rosea Púrpura, BRS Céu do Cerrado, BRS Roseflora, BRS Estrela do Cerrado, and BRS Pérola do Cerrado cultivars, 33 morphoagronomic descriptors, and two molecular markers were used. The categorical morphoagronomic descriptors were analyzed by frequency distribution and multivariate analyses. The quantitative morphoagronomic descriptors were subjected to the analysis of variance and to the comparison of the means of each cultivar. The random amplified polymorphic DNA (RAPD) and the inter-simple sequence repeats (ISSR) markers were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora; Molecular markers; Multivariate analysis; Plant cultivar protection.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001000849

Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD
Provedor de dados: 86
Autores: Andrade-Rodríguez,María; Villegas-Monter,Angel; Carrillo-Castañeda,Guillermo; García-Velázquez,Armando.
The objectives of this work were to evaluate the frequency of polyembryony, and to identify zygotic and nucellar seedlings of Citrus volkameriana using RAPD. Twenty-five polyembryonic and eight monoembryonic seeds were cultivated in vitrofor six months. DNA from seedlings was extracted and used in combination with five RAPD primers to identify zygotic or nucellar origin of the seedlings. Environmental conditions of the year affected significantly (P&lt;0.05) the morphological characteristics of fruitsand the number ofembryos per seed. Polyembryonic seeds ranged from 30.9%, 44.8% to 54.4% over three years. Morphological characteristic was not correlated with polyembryony. In vitro culture enable all embryos of each seed to grow, favoring the percentage of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus volkameriana; Rootstock; Embryo culture; Molecular markers; Polymorphism.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000600006

New microsatellite markers developed from an enriched microsatellite common bean library
Provedor de dados: 86
Autores: Cardoso,Juliana Morini Kupper; Oblessuc,Paula Rodrigues; Campos,Tatiana de; Sforça,Danilo Augusto; Carbonell,Sérgio Augusto Moraes; Chioratto,Alisson Fernando; Formighieri,Eduardo Fernandes; Souza,Anete Pereira de; Benchimol,Luciana Lasry.
The objective of this work was to develop new microsatellite markers in common bean. Ninety nine new microsatelitte loci were developed from a microsatellite enriched library for (CT)8 and (GT)8 motifs, from CAL-143 line. The majority of microsatellite sequences (51%) was related to cellular metabolism. The remaining sequences were associated to transcription functions. Only 17.2% of the sequences presented some level of similarity with other plant species genes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Molecular markers; Plant breeding; QTL; SSR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700019

Microsatellites for genetic studies and breeding programs in common bean
Provedor de dados: 86
Autores: Campos,Tatiana de; Benchimol,Luciana Lasry; Carbonell,Sérgio Augusto Moraes; Chioratto,Alisson Fernando; Formighieri,Eduardo Fernandes; Souza,Anete Pereira de.
Twenty microsatelitte loci were identified and characterized in common bean. Microsatellites were tested in 14 genotypes. The allele number ranged from 1 to 3, and the polymorphism information content (PIC) was between 0.14 and 0.65. These polymorphic markers are available to be used for breeding programs.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; SSR; Molecular markers.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000400018

Genetic variability of the Brazilian hair sheep breeds
Provedor de dados: 86
Autores: Paiva,Samuel Rezende; Silvério,Vanessa Chaves; Egito,Andréa Alves; McManus,Concepta; Faria,Danielle Assis de; Mariante,Arthur da Silva; Castro,Sílvia Ribeiro; Albuquerque,Maria do Socorro Maués; Dergam,Jorge Abdala.
The objectives of this work were to investigate the genetic structure of the Brazilian hair sheep breeds and to determine the origin of the Santa Inês breed. Molecular similarity was determined using Randomly Amplified Polymorphic DNA - Polymerase Chain Reaction markers in 238 individuals from five naturalized sheep breeds: Santa Inês (48 animals), Rabo Largo (48), Somali (48), Morada Nova (48) and Bergamasca (46), collected in Goiás, Sergipe, Bahia, and Ceará States as well as in the Federal District. Fifty-four loci were selected from 19 primers, after a pilot test using 140 primers. Qualitative analyses indicate diagnostic markers for all breeds. All breeds were significantly different from each other. Interbreed differences were explained by 14.92% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovis aries; Conservation genetics; Domestic animal diversity; Molecular markers; RAPD.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000900008

Molecular characterization of grapevine from Santa Catarina, Brazil, using microsatellite markers
Provedor de dados: 86
Autores: Schuck,Mariane Ruzza; Moreira,Flavia Maia; Guerra,Miguel Pedro; Voltolini,José Afonso; Grando,Maria Stella; Silva,Aparecido Lima da.
The objective of this work was to characterize the grape germplasm in Santa Catarina, Brazil, using microsatellite DNA markers (simple sequence repeats - SSR). The DNA samples were collected from leaves and shoots of accessions of public and private collections from the counties Urussanga, Nova Trento, Rodeio, São Joaquim, Campos Novos, Videira, and Água Doce. Ten SSR loci (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79, VVMD25, VVMD28, VVMD31, and VVMD32) were analysed by capillary electrophoresis. Molecular profiling was conducted for 190 grapevines (European, American, and hybrids), and 67 genotypes were obtained. The data were compared with each other and with those from the literature and from online databases, in order to identify varieties and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitis spp.; Cultivar identification; Genetic variability; Germplasm collection; Molecular markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000500008

Yield QTL analysis of Oryza sativa x O. glumaepatula introgression lines
Provedor de dados: 86
Autores: Rangel,Priscila Nascimento; Vianello,Rosana Pereira; Melo,Arthur Tavares Oliveira; Rangel,Paulo Hideo Nakano; Mendonça,João Antônio; Brondani,Claudio.
The objective of this work was to evaluate the yield performance of two generations (BC2F2 and BC2F9) of introgression lines developed from the interspecific cross between Oryza sativa and O. glumaepatula, and to identify the SSR markers associated to yield. The wild accession RS‑16 (O. glumaepatula) was used as donor parent in the backcross with the high yielding cultivar Cica‑8 (O. sativa). A set of 114 BC2F1 introgression lines was genotyped with 141 polymorphic SSR loci distributed across the whole rice genome. Molecular analysis showed that in average 22% of the O. glumaepatula genome was introgressed into BC2F1 generation. Nine BC2F9 introgression lines had a significantly higher yield than the genitor Cica‑8, thus showing a positive genome...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AB‑QTL; Breeding; Genetic pool; Molecular markers; Target genome region; Yield performance.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000300006

Artificial neural networks compared with Bayesian generalized linear regression for leaf rust resistance prediction in Arabica coffee
Provedor de dados: 86
Autores: Silva,Gabi Nunes; Nascimento,Moysés; Sant’Anna,Isabela de Castro; Cruz,Cosme Damião; Caixeta,Eveline Teixeira; Carneiro,Pedro Crescêncio Souza; Rosado,Renato Domiciano Silva; Pestana,Kátia Nogueira; Almeida,Dênia Pires de; Oliveira,Marciane da Silva.
Abstract: The objective of this work was to evaluate the use of artificial neural networks in comparison with Bayesian generalized linear regression to predict leaf rust resistance in Arabica coffee (Coffea arabica). This study used 245 individuals of a F2 population derived from the self-fertilization of the F1 H511-1 hybrid, resulting from a crossing between the susceptible cultivar Catuaí Amarelo IAC 64 (UFV 2148-57) and the resistant parent Híbrido de Timor (UFV 443-03). The 245 individuals were genotyped with 137 markers. Artificial neural networks and Bayesian generalized linear regression analyses were performed. The artificial neural networks were able to identify four important markers belonging to linkage groups that have been recently mapped,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica; Hemileia vastatrix; Artificial intelligence; Molecular markers; Prediction.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000300186

QTL mapping for protein content in soybean cultivated in two tropical environments
Provedor de dados: 86
Autores: Soares,Taís Cristina Bastos; Good-God,Pedro Ivo Vieira; Miranda,Fábio Demolinari de; Soares,Janaína Bastos; Schuster,Ivan; Piovesan,Newton Deniz; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves.
The objectives of this study were to detect quantitative trait loci (QTL) for protein content in soybean grown in two distinct tropical environments and to build a genetic map for protein content. One hundred eighteen soybean recombinant inbred lines (RIL), obtained from a cross between cultivars BARC 8 and Garimpo, were used. The RIL were cultivated in two distinct Brazilian tropical environments: Cascavel county, in Paraná, and Viçosa county, in Minas Gerais (24º57'S, 53º27'W and 20º45'S, 42º52'W, respectively). Sixty-six SSR primer pairs and 65 RAPD primers were polymorphic and segregated at a 1:1 proportion. Thirty poorly saturated linkage groups were obtained, with 90 markers and 41 nonlinked markers. For the lines cultivated in Cascavel, three QTL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Linkage map; Molecular markers; Recombinant inbred lines; Quantitative trait loci.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001100012

Genetic variability of hull-less barley accessions based on molecular and quantitative data
Provedor de dados: 86
Autores: Sayd,Ricardo Meneses; Amabile,Renato Fernando; Faleiro,Fabio Gelape; Bellon,Graciele.
The objective of this work was to characterize and quantify the genetic, molecular, and agronomic variability of hull-less barley genotypes, for the selection of parents and identification of genotypes adapted to the irrigated production system in the Brazilian Cerrado. Eighteen hull-less barley accessions were evaluated, and three covered barley accessions served as reference. The characterization was based on 157 RAPD molecular markers and ten agronomic traits. Genetic distance matrices were obtained based on molecular markers and quantitative traits. Graphic grouping and dispersion analyses were performed. Genetic, molecular, and agronomic variability was high among genotypes. Ethiopian accessions were genetically more similar, and the Brazilian ones...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hordeum vulgare; Genetic diversity; Molecular markers; Quantitative traits.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000200160

Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars
Provedor de dados: 86
Autores: Ochoa,Elisa del Carmen Martínez; Andrade-Rodríguez,María; Rodríguez,Mario Rocandio; Monter,Angel Villegas.
The objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangifera indica; Embryo culture; Funiculus; Molecular markers; Polymorphism.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012001100010

Optimization of heterologous microsatellites in Piracanjuba
Provedor de dados: 86
Autores: Carmo,Fausto Moreira da Silva; Polo,Érico Macedo; Silva,Marcos Antônio da; Yazbeck,Gabriel de Menezes.
Abstract: The objective of this work was to evaluate 41 microsatellite markers for heterologous amplifications in piracanjuba (Brycon orbignyanus). Some markers were tested for the first time. Loci were optimized for PCR conditions and applied to a sample of 49 individuals. Thirty-one loci resulted in PCR product formation, whereas ten loci yielded intelligible polymorphic patterns in the evaluated sample and can be used for amplifications in this species. From the evaluated markers, four loci (BoM1, BoM13, Bh6, and Bh16) are valid to be applied in the study of piracanjuba.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brycon orbignyanus; Aquaculture; Hatchery; Molecular markers.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015001201236

Molecular markers to study the variability within the Eragrostis curvula complex
Provedor de dados: 85
Autores: Zappacosta,D; Meier,M; Carrera,A; Pacheco,G; Cardone,S; Selva,JP; Echenique,V.
Weeping lovegrass is well adapted for forage production and useful for soil conservation in semiarid regions, constituting a morphologically diverse group. Diploid genotypes are unfrequent and reproduce sexually, whereas the tetraploids and plants of higher ploidy levels reproduce by apomixis. In the present work RAPD, AFLP and EST-SSR were used in order to assess the reproductive mode through progeny tests, to determine intracultivar homogeneity or seed purity, to establish genetic relationships among the cultivars within the complex and to characterize the new materials obtained by our group. Eight commercial cultivars and three new plant materials were analyzed. Uniform and variable patterns were observed in progenies of apomictic and sexual plants,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eragrostis; Molecular markers; Apomixis; Seed purity; Cultivar identification.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572011000200014

Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil
Provedor de dados: 87
Autores: Rocha,D.C.; Rodella,R.A.; Marino,C.L.; Martins,D.
This work aims to carry out a comparative analysis using RAPD molecular markers in four Commelina weed species from the state of Paraná and C. benghalensis populations from the states of Paraná and São Paulo, Brazil. The genomic plant DNA sample was extracted from the leaves, separated, randomly fragmented and amplified by PCR. Random amplified polymorphic DNA fragments (RAPD markers) were analyzed by using POPGENE statistical program. Eighty-five primer sequences were tested but only three were suitable as molecular markers producing 37 DNA polymorphic fragments for comparisons among four Commelina species and 22 polymorphic fragments for comparisons among C. benghalensis populations. The results showed that there were inter-specific and intra-specific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotype; Commelinaceae; Molecular markers; Weeds.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582009000300001

Laboratory Protocols CIMMYT Applied Molecular Genetics Laboratory
Provedor de dados: 31
Autores: Warburton,   Marilyn.
Third Edition
Tipo: Report
Palavras-chave: Chemiluminescence immunoassays; DNA; DNA hybridization; Molecular markers; PCR; RFLPs; Research and Development/Tech Change/Emerging Technologies; F30.
Ano: 2005
URL: http://purl.umn.edu/56184

Assessment of silver-stained AFLP markers for studying DNA polymorphism in proso millet (Panicum miliaceum L.)
Provedor de dados: 88
Autores: Karam,Décio; Westra,Philip; Niessen,Scott J.; Ward,Sarah M.; Figueiredo,José Edson Fontes.
Proso millet (Panicum miliaceum L.) is a serious weed in North America. A high number of wild proso millet biotypes are known but the genetic basis of its phenotypic variation is poorly understood. In the present study, a non-radioactive silver staining method for PCR-Amplified Fragment Length Polymorphism (AFLP) was evaluated for studying genetic polymorphism in American proso millet biotypes. Twelve biotypes and eight primer combinations with two/three and three/three selective nucleotides were used. Pair of primers with two/three selective nucleotides produced the highest number of amplified DNA fragments, while pair of primers with three/three selective nucleotides were more effective for revealing more polymorphic DNA fragments. The two better primer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Genetic diversity; Molecular markers; Proso-millet.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042006000400011

Characterization of selection effects on broiler lines using DNA fingerprinting
Provedor de dados: 89
Autores: Schmidt,GS; Hellmeister Filho,P; Zanella,EL.
The objective of this study was to evaluate the effect of selection for body weight on the genetic variability and diversity in broiler lines. Two paternal broiler lines (LL and LLc) were used. LL line was selected for 12 generations for growth and carcass and reproduction characteristics. The LLc line was established from LL line in 1985 and mated at random. Blood samples from six chickens per line were collected and used for molecular analysis. Also, a DNA pool was made for each line to compare effects between lines. Data were analyzed considering the collected information on the presence or absence of DNA bands. Band sharing scores were calculated using the DICE coefficient. The pattern of the 21 most representative bands was used. DNA fingerprinting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Body weight; Fingerprinting; Molecular markers; Variability.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000200005

Exon-primed intron-crossing (EPIC) markers as a tool for ant phylogeography
Provedor de dados: 92
Autores: Ströher,Patrícia R.; Li,Chenhong; Pie,Marcio R.
Exon-primed intron-crossing (EPIC) markers as a tool for ant phylogeography. Due to their local abundance, diversity of adaptations and worldwide distribution, ants are a classic example of adaptive radiation. Despite this evolutionary and ecological importance, phylogeographical studies on ants have relied largely on mitochondrial markers. In this study we design and test exon-primed intron-crossing (EPIC) markers, which can be widely used to uncover ant intraspecific variation. Candidate markers were obtained through screening the available ant genomes for unlinked conserved exonic regions interspersed with introns. A subset of 15 markers was tested in vitro and showed successful amplification in several phylogenetically distant ant species. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Formicidae; Molecular markers; Phylogeny; Population genetics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262013000400014

PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae)
Provedor de dados: 92
Autores: Calado,Daniéla; Navarro-Silva,Mario Antonio; Sallum,Maria Anice Mureb.
The aim of the present study was to examine genetic variability in populations of An. cruzii by employing PCR-RAPD and PCR-RFLP markers. All analyses were carried out using individuals of the F1 generation of wild caught females obtained in Santa Catarina State (Florianópolis and São Francisco do Sul), Paraná State (Morretes, Paranaguá and Guaratuba) and São Paulo State (Cananéia). In the PCR-RAPD experiments, seven primers were used for comparisons within and among populations. The restriction profile of the ITS2 including a fragment of both 5.8S and 28S regions of the rDNA was obtained with the enzymes BstUI, HaeIII, TaqI, HhaI, Sau96I, HinfI, HincII and NruI. The PCR-RAPD method detected a large number of polymorphic bands. Genetic distance among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anophelinae; Genetic variability; ITS2; Molecular markers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262006000300014

Advances in research and development of mango industry
Provedor de dados: 94
Autores: Bally,Ian S. E.
World mango production is spread over 100 countries that produce over 34.3 million tons of fruit annually. Eighty percent of this production is based in the top nine producing nations that also consume upward of 90% of their production domestically. One to 2 percent of fruit is traded internationally in to markets in the European Community, USA, Arabian Peninsula and Asia. This paper outlines some of the recent research and development advances in mango breeding and genomics, rootstock development, disease management and harvest technologies that are influencing the production and quality of mango fruit traded domestically and internationally.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Australia; Breeding; Molecular markers; Rootstocks; Anthracnose; Harvesting; Sap-burn.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000500008

Morphologic and molecular characterization of Myrciaria spp species
Provedor de dados: 94
Autores: Pereira,Márcio; Oliveira,Antonio Luis de; Pereira,Regina Eli de Almeida; Sena,Janete Apparecida Desidério; Costa,Juliana Regina Vieira da; Almeida,Marcílio de; Gonçalves,Antonio Natal.
The jabuticaba tree is considered one of the most typical Brazilian fruit trees. However, few studies of this plant are found in the literature and even its botanical classification is very controversial. The present research reports some comparisons between jabuticaba species, using morphologic (organography) and molecular markers techniques. The morphologic characteristics of the plant used as markers were compared with specimens of the herbaria from São Paulo and Minas Gerais States and with the descriptions reported in the literature. Molecular differences between the species were identified by the use of RAPD markers. The experiment was made in Piracicaba, Jaboticabal and Ituverava municipal districts in São Paulo State, Brazil. Morphologic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jabuticaba trees; Biological identification; Morphologic markers; Molecular markers; RAPD.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452005000300040

GENETIC DIVERSITY AND STRUCTURE OF Oenocarpus mapora GERMPLASM CONSERVED AT EASTERN AMAZON
Provedor de dados: 94
Autores: MOURA,ELISA FERREIRA; OLIVEIRA,MARIA DO SOCORRO PADILHA DE; SILVA,DIEHGO TULOZA DA; PONTES,LÍGIA CRISTINE GONÇALVES.
ABSTRACT  The aim of this study was to evaluate the genetic diversity and structure in the germoplasm of Oenocarpus mapora conserved at Eastern Amazon. Thus, 88 individuals were genotyped with five microsatellite loci. These individuals belong to 24 accessions that were sampled in eight sample places of three Brazilian Amazon states conserved at the Active Germplasm Bank (AGB) of Embrapa Eastern Amazon. All loci were polymorphic and they generated 85 alleles with an average of 17 alleles per loci. Total genetic diversity (HE) was 0.48. Sample places were considered genetically distinct, with ?p = 0.354. The analysis of molecular variance (AMOVA) identified that the genetic portion among areas was of 36.14% and within 63.86%. The Nei distances varied from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arecaceae; Molecular markers; Microsatellites; Plant genetic resources.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452015000400984

Sequence-characterized amplified region (SCAR) technique used for variety discrimination in vinca (Catharanthus roseus (L.) G.Don)
Provedor de dados: 95
Autores: Menezes,Carlos C.E; Vantoai,Tara; McDonald,Miller B; Sediyama,Tocio.
Sequence-Characterized Amplified Region (SCAR) appears as a useful technique for genetic purity testing and variety discrimination, applicable to species in which some other techniques have failed. In particular, this technique is very attractive with species in which RAPD results were not consistent. The RAPD polymorphic bands were cloned, sequenced and from the sequence information, primers pairs for normal PCR were developed. Since the probability of obtaining successful SCAR primers from RAPD polymorphic bands was about 50%, a larger number of RAPD polymorphic bands are needed to develop sufficient SCAR primers for varietal discrimination in vinca. In addition, the efficiency of the SCAR technique is strongly affected by the quality of DNA extracted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vinca; Seed; Variety discrimination; Molecular markers; SCAR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222002000100041

Hybrids between Pseudoplatystoma corruscans and P. reticulatum (Siluriformes: Pimelodidae) previously reported in the Upper Paraná River are likely escapes from aquaculture farms: evidence from microsatellite markers
Provedor de dados: 103
Autores: Baggio,Rafael A.; Moretti,Caroline B.; Bialetzki,Andréa; Boeger,Walter A.
ABSTRACT The production of hybrids of the 'pintado', Pseudoplatystoma corruscans (Spix &amp; Agassiz, 1829) and 'cachara', Pseudoplatystoma reticulatum (Eigenmann &amp; Eigenmann, 1889) in captivity has generated many concerns about the possibility of introduction of farmed hybrids into natural environments. In the last decade, hybrids between these species, known as 'pintachara' or 'cachapinta', were reported from different regions of the Upper Paraná River basin. Prospection of these hybrids is important in order to orient conservation programs for the species involved. Knowledge of the presence of these hybrids will direct conservation strategies towards prevention and/or mitigation of the effects of cross breeding in natural populations of P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; Fish larvae; Interspecific hybrids; Introgression; Molecular markers.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702016000201303

HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle
Provedor de dados: 96
Autores: Baena,Marielle Moura; Tizioto,Polyana Cristiane; Meirelles,Sarah Laguna Conceição; Regitano,Luciana Correia de Almeida.
ABSTRACT The purpose of this study was to access and characterize single nucleotide polymorphisms (SNP) located within the HSF1 and HSPA6 candidate genes for adaptability in Angus breed raised in subtropical climate. Samples of DNA from 20 animals representing extreme phenotypes for adaptability traits were obtained. Sequence variations in the candidate genes were described by sequencing target regions. We identified 12 SNP located in the HSF1 gene. Moreover, four of the six SNP found in the HSPA6 gene cause amino acid substitutions in protein-coding regions. We also identified a representative SNP (called tag SNP) in a region of the HSF1 gene with high linkage disequilibrium (r2 = 0.87) that may represent 11 SNP located in this gene. Minor allele...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angus; Molecular markers; Thermoregulation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100402

Genetics and breeding of sheep in Brazil
Provedor de dados: 96
Autores: McManus,Concepta; Paiva,Samuel Rezende; Araújo,Ronyere Olegário de.
Studies in genetics and breeding of sheep in Brazil have increased significantly in recent years. These involve research in characterization, breeding and crossing sheep using new technologies available incorporating both classical quantitative and molecular genetics. Improvements in statistical techniques, computational resources as well as analysis of DNA and gaps in present knowledge and opportunities for possible research are pointed out. There is a need for greater interaction between various groups working in the country as well as interactions with other disciplines such as Geographical Information Systems, Statistics, Bioinformatics, as well as biological studies such as physiology and proteomics.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breed; Disease; Heat tolerance; History; Molecular markers; Ovis aries.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300026

A note on the distribution of genetic diversity of Anglo-Nubian goats in central-northern farms of Piauí, Brazil
Provedor de dados: 96
Autores: Silvestre,Ellida de Aguiar; Costa,Márcio da Silva; Silva,Pollyana Oliveira da; Bajay,Miklos Maximiliano; Pinheiro,José Baldin; Zucchi,Maria Imaculada; Campelo,José Elivalto Guimarães; Britto,Fábio Barros.
The objective of this study was to characterize the genetic variability of Anglo-Nubian goats using microsatellite markers. The study was conducted using herds from four municipalities of Central-Northern Piauí (Teresina, José de Freitas, Campo Maior, and Angical), where technical information is scarce. Seven markers suggested by FAO were used (ILSTS11, McM527, INRA23, ETH10, OarfCB304, OarfCB48, and MAF209). The samples were genotyped using a 7% polyacrylamide gel. The average number of alleles per locus was 4.0, with observed and expected heterozygosity of 0.38 and 0.55, respectively. Few deviations from the Hardy-Weinberg equilibrium were observed for each population. Only two loci deviated significantly in two localities. The coefficient of gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endogamy; Genetic structure; Microsatellite; Molecular markers.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015000400155

Molecular characterisation and similarity relationships among iranian basil (Ocimum basilicum L.) accessions using inter simple sequence repeat markers
Provedor de dados: 124
Autores: Aghaei,Mohammad; Darvishzadeh,Reza; Hassani,Abbas.
The study of genetic relationships is a prerequisite for plant breeding activities as well as for conservation of genetic resources. In the present study, genetic diversity among 50 Iranian basil (Ocimum basilicum L.) accessions was determined using inter simple sequence repeat (ISSR) markers. Thirty-eight alleles were generated at 12 ISSR loci. The number of alleles per locus ranged from 1 to 5 with an average of 3.17. The maximum number of alleles was observed at the A7, 818, 825 and 849 loci, and their size ranged from 300 to 2500 bp. A similarity matrix based on Jaccard's coefficient for all 50 basil accessions gave values from 1.00-0.60. The maximum similarity (1.00) was observed between the "Urmia" and "Shahr-e-Rey II" accessions as well as between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Basil; Cluster analysis; Molecular markers; Plant genetic diversity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902012000200014

SSR markers to detect gene flow from upland to mocó cotton
Provedor de dados: 124
Autores: Dantas,Ana Carolina Assis; Barroso,Paulo Augusto Vianna; Hoffmann,Lúcia Vieira; Alves,Milena Ferreira; Andrade,Francisco Pereira de.
Mocó cotton was the most important crop in Northeastern Brazil. Local varieties had been developed before governmental breeding programs, which should be identified and preserved as a genetic resource. The aim of this work was to select SSR markers which can be used to monitor gene flow from herbaceous to mocó cotton plants, using non transgenic upland cotton as a pollen source. Unique mocó cotton feral populations were identified in the state of Rio Grande do Norte, and mother plants and their seeds were collected. Allele frequencies were studied among fifteen plants from these populations using 64 SSR primer pairs. Ten SSR markers, with alleles exclusive to mocó or upland genotypes, were selected to monitor gene flow by paternity analysis (BNL3627; 2960;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosafety; Molecular markers; Genetic resources.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902012000100020

Genetic diversity of cultivated Coffea arabica inbred lines assessed by RAPD, AFLP and SSR marker systems
Provedor de dados: 63
Autores: Maluf,Mirian Perez; Silvestrini,Milene; Ruggiero,Luciana Machado de Campos; Guerreiro Filho,Oliveiro; Colombo,Carlos Augusto.
One of the greatest problems in Coffea arabica breeding is identifying precisely any inbred line, based only on botanical and agronomical descriptors, because of the reduced genetic variability of the species, close pedigree origin, which results in small phenotypic variation. Recently, molecular markers have been used for plant germplasm characterization and identification in several commercial species. This work evaluates the reliability of three marker systems: RAPD, AFLP and SSR, to characterize the genetic variability of commercially-used Coffea inbred lines developed by the Instituto Agronômico (IAC), and their potential for cultivar identification. All methods identified polymorphisms among the cultivars. The genetic diversity recognized by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Cultivar identification; Molecular markers.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000400010

Pilocarpine content and molecular diversity in jaborandi
Provedor de dados: 63
Autores: Sandhu,Sardul Singh; Abreu,Ilka Nacif; Colombo,Carlos Augusto; Mazzafera,Paulo.
Pilocarpine is an imidazol alkaloid exclusively found in Pilocarpus genus and P. microphyllus accumulates its highest content in the leaves. There is no report in the literature on the variability of the pilocarpine content in this genus. A population of 20 genotypes of P. microphyllus from the state of Maranhão, Brazil, was analyzed for Random Amplification of Polymorphic DNA (RAPD) markers and pilocarpine content. Although it was not possible to establish any correlation between these features, the absence or presence of some markers could indicate in some genotypes a possible association with the content of the alkaloid.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RAPD; Pilocarpus jaborandi; Molecular diversity; Molecular markers.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000500010

Agronomic and molecular characterization of wild germplasm Saccharum spontaneum for sugarcane and energycane breeding purposes
Provedor de dados: 63
Autores: Silva,Jorge Alberto Gonçalves da; Costa,Paulo Mafra de Almeida; Marconi,Thiago Gibbin; Barreto,Everton Jean da Silva; Solís-Gracia,Nora; Park,Jon-Won; Glynn,Neil Charles.
ABSTRACT: Among the species related to sugarcane, Saccharum spontaneum (L.) is a wild species with the greatest potential as a source of genetic variation to cope with biomass production in harsh environments. Due to its high yield, early vigor, ratooning ability, low input requirements and tolerance to various biotic and abiotic stresses, sugarcane breeders have shown interest in its contribution, as a donor of genes, to the development of high biomass energy canes. The conservation, evaluation and utilization of the genetic variability of S. spontaneum available in germplasm collections are critical for breeding, but, given the aggressive rhizomatous growth habit and the ability to propagate via seed dispersal, S. spontaneum is classified as a noxious...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Yield; Bioenergy; Abiotic stress; Core collection.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000400329

Association of CSSM066 and ILSTS011 microsatellite markers and thyroglobulin gene SNP with backfat in Canchim cattle
Provedor de dados: 63
Autores: Veneroni,Gisele Batista; Meirelles,Sarah Laguna; Oliveira,Henrique Nunes de; Alencar,Maurício Mello de; Gasparin,Gustavo; Gouveia,João José de Simoni; Cervini,Marcelo; Regitano,Luciana Correia de Almeida.
Canchim, a synthetic breed of cattle derived from the Charolais and Zebu group has been used in the beef-cattle industry in Brazil as an alternative for intensifying production. One of the main concerns with this breed is its poor fat deposition and consequently, there is an effort to increase the performance for this trait. The thyroglobulin gene is located in a QTL region for fat deposition, and reports describe the influence of a polymorphism in the 5´ leader sequence of that gene on marbling and subcutaneous fat thickness. This study analyzed the association of this polymorphism in the thyroglobulin gene, as well as of two flanking microsatellite markers, CSSM066 and ILSTS011, with backfat thickness in 987 Canchim beef cattle. The CSSM066 and ILSTS011...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canchim breed; Fat deposition; Molecular markers; Candidate gene.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000100001

Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers
Provedor de dados: 63
Autores: Morales,Rafael Gustavo Ferreira; Resende,Juliano Tadeu Vilela; Faria,Marcos Ventura; Andrade,Marcela Carvalho; Resende,Luciane Vilela; Delatorre,Carla Andrea; Silva,Paulo Roberto da.
Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fragaria× ananassa; Plant breeding; Genetic divergence; Molecular markers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000600010

Genetic structure of annual ryegrass (Lolium multiflorum) populations estimated by RAPD
Provedor de dados: 63
Autores: Vieira,Eduardo Alano; Castro,Caroline Marques; Oliveira,Antônio Costa de; Carvalho,Fernando Irajá Félix de; Zimmer,Paulo Dejalma; Martins,Luís Fernando.
Annual ryegrass is a temperate climate annual foraging grass, grown mostly in the South of Brazil, especially in the state of Rio Grande do Sul. Despite its importance, little is known about its genetic diversity, both within and among the populations cultivated. This knowledge is of fundamental importance for developing breeding and conservation strategies. The aim of this study was to characterize the genetic diversity and structure of four populations of annual ryegrass. Three of the populations were located in Rio Grande do Sul and the fourth in Uruguay. RAPD markers were used to study the genetic diversity and structure of these populations. Analysis of 375 individuals sampled from the populations, using six RAPD primers, generated a total of 82...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Foraging; Genetic diversity; Molecular markers.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400009

Receptor-Like Kinase (RLK) as a candidate gene conferring resistance to Hemileia vastatrix in coffee
Provedor de dados: 63
Autores: Almeida,Dênia Pires de; Castro,Isabel Samila Lima; Mendes,Tiago Antônio de Oliveira; Alves,Danúbia Rodrigues; Barka,Geleta Dugassa; Barreiros,Pedro Ricardo Rossi Marques; Zambolim,Laércio; Sakiyama,Ney Sussumu; Caixeta,Eveline Teixeira.
ABSTRACT: The biotrophic fungus Hemileia vastatrix causes coffee leaf rust (CLR), one of the most devastating diseases in Coffea arabica . Coffee, like other plants, has developed effective mechanisms to recognize and respond to infections caused by pathogens. Plant resistance gene analogs (RGAs) have been identified in certain plants as candidates for resistance ( R ) genes or membrane receptors that activate the R genes. The RGAs identified in different plants possess conserved domains that play specific roles in the fight against pathogens. Despite the importance of RGAs, in coffee plants these genes and other molecular mechanisms of disease resistance are still unknown. This study aimed to sequence and characterize candidate genes from coffee plants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica; Coffee leaf rust; Resistance gene analogs; Molecular markers; Plant breeding.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000601101

Microsatellite markers for identification of a group of italian olive accessions
Provedor de dados: 63
Autores: Muzzalupo,Innocenzo; Stefanizzi,Francesca; Salimonti,Amelia; Falabella,Rosanna; Perri,Enzo.
Cultivar characterization for fruit trees certification requires fast, efficient and reliable techniques. Microsatellite markers (SSR) were used in the molecular characterization of 23 genotypes of Olea europaea subsp europaea. The DNA from the olive cultivars was analyzed using nine pre-selected SSR primers (GAPU59, GAPU71A, GAPU71B, GAPU103A, UDO99-01, UDO99-12, UDO99-28 and UDO99-39) and revealed 29 alleles, which allowed each genotype to be identified. In the dendrogram, the nine primers allowed the 23 olive genotypes to be grouped into subgroups corresponding to the same cultivar denominations. SSR markers proved to be efficient and reliable for the molecular characterization of Italian olive cultivars.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olea europaea; Fingerprinting; Genetic identity; Molecular markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500014

Diversity and genetic structure of jenipapo (Genipa americana L.) Brazilian accessions
Provedor de dados: 63
Autores: Silva,Ana Veruska Cruz da; Freire,Karla Cristina Santos; Lédo,Ana da Silva; Rabbani,Allívia Rouse Carregosa.
Usually known as jenipapo, Genipa americana L. is a Rubiaceae Brazilian native species. It is an important species in the restoration of Brazilian riparian forests and is one of the most promising fruit trees for sustainable harvesting programs. In this study we provide the first data on the genetic diversity and structure of a Jenipapo Germplasm Bank using inter simple sequence repeat (ISSR) markers. We evaluated 160 accessions from wild populations, and the data generated from 12 ISSR primers were used to determine genetic variability via a model-based Bayesian procedure (Structure) and molecular variance analysis. In addition, Shannon index, genetic diversity and Jaccard coefficients were estimated. A total of 12 primers were used, which generated 123...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tropical fruits; Molecular markers; Germplasm; Conservation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000500007

Microsatellite analysis of pacu broodstocks used in the stocking program of Paranapanema River, Brazil
Provedor de dados: 63
Autores: Povh,Jayme Aparecido; Ribeiro,Ricardo Pereira; Lopera-Barrero,Nelson Mauricio; Jacometo,Carolina Bespalhok; Vargas,Lauro; Gomes,Patrícia Cristina; Lopes,Taís da Silva.
Monitoring the genetic diversity has fundamental importance for fish stocking programs. This experiment aims to evaluate the genetic diversity in two hatchery stations (A and B) with pacu Piaractus mesopotamicus (Holmberg, 1887) in Andirá, state of Paraná, Brazil used in stocking programs of Paranapanema River. Six microsatellite loci were amplified using DNA extracted from 60 fin-clipping samples. The broodstock B had the average number of alleles and the mean heterozygosity (alleles: 3.7 and H O: 0.628) higher than the broodstock A (alleles: 3.5 and H O: 0.600). Alleles with low frequency levels were observed in the both broodstocks. The positive coefficients of endogamy in the locus Pme2 (broodstock A: F IS = 0.30 and broodstock B: F IS = 0.20), Pme5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piaractus mesopotamicus; Genetic variability; Hatchery stations; Molecular markers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300006

Use of molecular marker techniques in seed testing by Brazilian seed companies
Provedor de dados: 63
Autores: Della Vecchia,P.T.; Silva,C.A.R. da; Terenciano-Sobrinho,P.
Seed market is becoming global and globalization is growing very fast. To compete favourably in this new global seed world, quality and cost are and will be certanly the key issues. High seed quality can only be obtained by a thorough control of the entire seed production process, step by step from planning to final delivery. That requires science, technology, expertise, experience, good management and certanly, the most important, an absolute and unconditional commitment with quality. Seed testing for quality assurance is one important step in the process of production of high quality seed. In the late years a considerable amount of research has been published, particularly on the use of some Polymerase Chain Reaction DNA based new technologies (RAPD,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Seed testing; Seed companies; Genetic purity.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000500014

Use of AFLPS to distinguish landraces of pejibaye (Bactris gasipaes) in brazilian Amazonia
Provedor de dados: 63
Autores: Clement,Charles R.; Sousa,Nelcimar Reis; Rodrigues,Doriane Picanço; Astolfi-Filho,Spártaco; Moreno,Yolanda Núñez; Pascual,Vicente Torres; Rodríguez,Francisco Javier Gallego.
Although the first inhabitants of western Amazonia domesticated pejibaye (Bactris gasipaes Kunth, Palmae) or peach palm for its fruits, today it is widely planted for its heart-of-palm. Like other domesticates, pejibaye presents a complex hierarchy of landraces developed before the conquest of the Americas. The existence of three landraces (Pará, Solimões, Putumayo) was proposed along the Amazonas and Solimões Rivers, Brazil, based on morphological characteristics. There are some questions remaining about the intermediate landrace being an artifact of the morphometric analysis. AFLPs were used to evaluate the relationships among samples of these putative landraces. DNA was extracted from 99 plants representing 13 populations maintained in the Pejibaye...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Population differentiation; Genetic resources; Genetic analysis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000400019

Variance additivity of genetic populational parameter estimates obtained through bootstrapping
Provedor de dados: 63
Autores: Carlini-Garcia,Luciana Aparecida; Vencovsky,Roland; Coelho,Alexandre Siqueira Guedes.
Studying the genetic structure of natural populations is very important for conservation and use of the genetic variability available in nature. This research is related to genetic population structure analysis using real and simulated molecular data. To obtain variance estimates of pertinent parameters, the bootstrap resampling procedure was applied over different sampling units, namely: individuals within populations (I), populations (P), and individuals and populations simultaneously (I, P). The considered parameters were: the total fixation index (F or F IT), the fixation index within populations (f or F IS) and the divergence among populations or intrapopulation coancestry (theta or F ST). The aim of this research was to verify if the variance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population structure; Resampling; Molecular markers; Natural populations; Simulation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000100015

การจัดทำลายพิมพ์เอกลักษณ์ดีเอ็นเอข้าวพื้นเมืองไทยที่เก็บอยู่ในธนาคารเชื้อพันธุ์ข้าวแห่งชาติ
Provedor de dados: 106
Autores: Payorm Cobelli; Varapong Chamarerk; Poonsak Mekwatanakarn; Phikul Leelagud; Somsong Chotechuen; Sommai Seewisut.
SSR molecular markers SSR are tandem arranged repeats of short DNA motifs (1-6 bp in length) distributed throughout the genome that frequently exhibit variation in the number of repeats at the locus of each rice variety. The SSR markers, therefore, are suitable for detecting polymorphisms between closely related genotypes of Thai local rice varieties from the National Rice Germplasm Bank. In this research, DNA fingerprinting was conducted using 8 Thai local rice varieties. i.e., Niaw Sanpahtawng (NSPT_GB), Khao Gaw Diaw (KGD_GB), Niaw Dam (ND_GB), Khao tah Haeng 17 (KTH17_GB), Jao’ Haw (JH_GB), Pin Gaew 56 (PG56_GB), Jek Chuey Gahb Khiaw (JCGK_GB), Sang Yod Pattalung (SYPL_GB), and 5 standard varieties, i.e., Khao Dawk Mali 105 (KDML105_GB, KDML105_C),...
Tipo: PhysicalObject
Palavras-chave: Thai local rice; DNA fingerprinting; Molecular markers; National Rice Germplasm Bank; ข้าวพื้นเมืองไทย; ลายพิมพ์เอกลักษณ์ดีเอ็นเอ; เครื่องหมายโมเลกุล; การเรียงตัวของดีเอ็นเอ; ความใกล้ชิดทางพันธุกรรม; พันธุ์.
Ano: 2012
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5225

การปรับปรุงพันธุ์ข้าวให้ทนดินเค็มและมีคุณภาพการหุงต้มดี
Provedor de dados: 106
Autores: Duangjai Suriyaarunroj; Apichart Vanavichit; Somvong Tragoonrung; Theerayut Toojinda.
Breeding for salt tolerant rice has been done for a decade. However, most of salt tolerant lines which have been released carry inferior cooking qualities compared to KDML105, an aroma good cooking quality rice of Thailand. To develop salt tolerant and good cooking quality rice, molecular marker assisted selection in backcross breeding program was initiated in 2001. Two RILs, FL496 and FL530 selected from mapping population of IR29 x Pokkali were used as salt tolerant donors. KDML105 as recipient was cross pollinated with these 2 donors to develop F1 and cross back to KDML105 to develop BC1F1. Three markers ; RM140, B1.1-1 and B1.1-11 flanking salt tolerant QTLs spanning 33 cM and two markers; RM00 and 10L03FW for amylose content and aroma scent were used...
Tipo: Collection
Palavras-chave: Salt tolerant rice; Molecular markers; Cooking quality; Yield; ข้าวทนเค็ม; โมเลกุลเครื่องหมาย; คุณภาพการหุงต้ม; ผลผลิต; การปรับปรุงพันธุ์.
Ano: 2007
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5301

Molecular characterization of Brazilian Dicyma pulvinata isolates
Provedor de dados: 102
Autores: Tavares,Eder T.; Tigano,Myrian S.; Mello,Sueli C. M.; Martins,Irene; Cordeiro,Celia M. T.
Forty-nine Brazilian Dicyma pulvinata isolates were examined by morphological traits and RFLP, RAPD and AFLP analyses. This fungus is a mycoparasite of Microcyclus ulei, the causal agent of the most devastating rubber (Hevea brasiliensis) disease, known as "South American Leaf Blight" (SALB). These isolates were compared with an Indian isolate from Cercosporidium sp., and a French isolate from Cladosporium fulvum. They were also compared with Dicyma ampullifera from Papua New Guinea. The morphological parameters analyzed confirmed the identification of the Brazilian isolates. The graphic representations of the distance matrices of each molecular marker showed similar results. Dicyma pulvinata isolates from M. ulei were closely related, whereas the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungi; Molecular markers; Biological control.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000200005

Identification of rapd marker linked to blast resistance gene in a somaclone of rice cultivar Araguaia
Provedor de dados: 102
Autores: ARAÚJO,LEILA G.; PRABHU,ANNE S.; FILIPPI,MARTA C.
The gene Pi-ar confers resistance to Pyricularia grisea race IB-45 in a somaclone derived from immature panicles of the susceptible rice (Oryza sativa) cultivar Araguaia. RAPD technique was used to identify molecular markers linked to this gene utilizing bulked segregant analysis. Initially, the two parental DNAs from the resistant donor SC09 and 'Araguaia' were analyzed using random primers. Of the 240 primers tested, 203 produced amplification products. The two parental DNAs along with the resistant and susceptible bulks of F2 population were screened using 48 primers that differentiated resistant and susceptible parents. Even though eight primers differentiated the resistant bulk from the susceptible bulk, as well as somaclone SC09 and 'Araguaia', only...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Disease resistance; Somaclonal variation; Pyricularia grisea.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000200010

Variability in ribosomal DNA genic and spacer regions in Verticillium dahliae isolates from different hosts
Provedor de dados: 102
Autores: Otero,Laura; Ducasse,Daniel; Miller,Robert N. G.
Using PCR-based assays with specific primers for amplification of the ribosomal DNA intergenic spacer region (IGS) and a portion of the mitochondrial DNA small subunit ribosomal RNA gene (mtDNA SSU rRNA), the genetic variability among Verticillium dahliae isolates from olive (Olea europaea) and other host species from Argentina and Brazil was estimated. The derived UPGMA-generated phenograms based upon the restriction fingerprinting data of rDNA IGS products revealed genetic differences, correlating with the host of origin. Isolates infecting olive genetically distinct from those from cocoa (Theobroma cacao) and sunflower (Helianthus annuus). Digestion of mitochondrial DNA SSU rRNA PCR products revealed less variability, distinguishing only one isolate...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Olea europaea; Molecular markers; Characterization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000400015

AFLP markers differentiate isolates of Colletotrichum gossypii from C. gossypii var. cephalosporioides
Provedor de dados: 102
Autores: Silva-Mann,Renata; Vieira,Maria G. G. Carvalho; Machado,José C.; Bernardino Filho,José Roberto; Salgado,Kalinka C. C.; Stevens,Mikel R.
Fungal diseases in cotton (Gossypium hirsutum), such as anthracnose caused by Colletotrichum gossypii and ramulose caused by C. gossypii var. cephalosporioides, are responsible for large yield losses. These pathogens are seed borne and morphologically similar although they induce different symptoms, which can lead to misdiagnosis using the blotter testing method. The present study was carried out to assess the viability of using Amplified Fragment Length Polymorphism (AFLP) markers to differentiate these pathogens. Five isolates, for each pathogen, were classified according to pathogenicity on cotton plants, and mycelial growth morphology. Conidial suspensions were sprayed on 30-day-old cotton plants and the symptoms assessed ten and 40 days after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Anthracnose; Ramulose; Cotton diseases; Seed borne pathogens.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200011

Genetic and phenotypic characterization of isolates of Pyricularia grisea from the rice cultivars epagri 108 and 109 in the State of Tocantins
Provedor de dados: 102
Autores: PRABHU,ANNE S.; FILIPPI,MARTA C.; ARAUJO,LEILA G.; FARIA,JOSIAS C.
An epidemic of rice (Oryza sativa) blast occurred on cultivars Epagri 108 and 109 in the municipalities of Lagoa da Confusão and Duerê in the State of Tocantins, during the rice-growing season 1998-99. DNA fingerprinting and virulence phenotype analysis were utilized to determine the diversity of Pyricularia grisea isolates collected from these cultivars in one epidemic year. Rep-PCR analysis of isolates was done by using two primer sequences from Pot2. Two distinct fingerprint groups or lineages were identified among 53 isolates collected from nine different commercial fields. The virulence pattern of isolates retrieved from these two cultivars was analyzed in artificial inoculation tests utilizing 32 genotypes in the greenhouse. A dendrogram constructed...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Magnaporthe grisea; Molecular markers; Oryza sativa; Rep-PCR analysis; Rice blast.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000600002

Plant pre-breeding for increased protein content in soybean Glycine max (L.) Merrill
Provedor de dados: 109
Autores: Silva-Rodrigues,Josiane Isabela da; Antunes-Arruda,Klever Márcio; Deniz-Piovesan,Newton; Gonçalves-de Barros,Everaldo.
Abstract The aim of this research were to determine protein content and genetic divergence of soybean genotypes in QTL regions of the trait grain protein content for plant breeding purposes. Twenty-nine soybean genotypes were grown in the field in four environments (Viçosa-MG Dec/2009, Visconde do Rio Branco-MG Feb/2010, São Gotardo-MG Feb/2010, and São Gotardo-MG Oct/2011) using the randomized block design with three replicates. The grain protein content was quantified by the infrared spectrometry method. The genetic divergence was estimated by the analysis of 39 microsatellite markers from QTL regions for the soybean grain protein content. The pairs of genotypes with greater genetic distances and protein contents were as follows: BARC-8/CS3032PTA276-3-4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant breeding; Molecular markers; QTL; Soybean genotypes; Protein contein.
Ano: 2017
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000400618

Identification of duplicates of cassava accessions sampled on the North Region of Brazil using microsatellite markers
Provedor de dados: 36
Autores: Moura,Elisa Ferreira; Farias Neto,João Tomé de; Sampaio,José Edson; Silva,Diehgo Tuloza da; Ramalho,Girena Fernandes.
Duplicates are common in germplasm banks and their identification is needed to facilitate germplasm bank management and to reduce maintenance costs. The aim of this work was to identify duplicates of cassava from a germplasm bank in Eastern Amazon, which had been previously characterized both morphological and agronomically. In order to be genotyped with 15 microsatellite loci, 36 accessions were selected. These accessions were classified into 13 groups of similar morpho-agronomical characteristics. All loci were polymorphic, and 75 alleles were identified, with an average of five alleles per loci and H E = 0.66. There were determined 34 pairs of genotypes with identical multiloci profiles and the probability of genetic identity was 1.1x10-12 with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Germplasm; Molecular markers.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672013000400008

Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases
Provedor de dados: 39
Autores: Pati,Prakash G; Datta,Subhojit; Agbagwa,Ikechukwu O; Singh,Indra Prakash; Soren,Khela Ram; Das,Alok; Choudhary,Arbind Kumar; Chaturvedi,Sushil Kumar.
Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP-RGA; Cajanus cajan; Genetic diversity; Molecular markers; Polymorphism.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200007

Genetic diversity in populations of Maytenus dasyclada (Celastraceae) in forest reserves and unprotected Araucaria forest remnants
Provedor de dados: 39
Autores: Reichmann,Manuel Castilhos; Zanella,Camila Ângela; Valério Júnior,Cláudio; Borges,Ana Claudia Piovezan; Sausen,Tanise Luisa; Paroul,Natalia; Mielniczki-Pereira,Albanin Aparecida; Teixeira,Amito José; Budke,Jean Carlos; Mossi,Altemir José; Cansian,Rogério Luis.
ABSTRACT Understanding the genetic structure and diversity of plants is fundamental to their conservation and permits their sustainable use by local communities. The genus Maytenus (Celastraceae) is composed of plants possessing pharmacological and antioxidant properties. However, the genetic and economic properties of the species M. dasyclada, a typical species of Araucaria forests in Brazil and Uruguay, have been little studied. In this work, the genetic structure and diversity of natural populations of M. dasyclada located in unprotected and preserved forest remnants were investigated using RAPD and isozymes markers. The results demonstrated that in areas of preservation, populations of M. dasyclada possess a relatively high degree of polymorphism and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Forest reserves; Genetic diversity; Maytenus dasyclada; Molecular markers.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000100093

Genetic diversity among Brazilian okra landraces detected by morphoagronomic and molecular descriptors
Provedor de dados: 1
Autores: Massucato, Luana Rainieri; Nakamura, Karina Kazue; Ruas, Paulo Mauricio; Zefa, Douglas Mariani; Silva, Derly José Henrique da; Gonçalves, Leandro Simões Azeredo.
The conservation of okra landraces [Abelmoschus esculentus (L.) Moench] in gene banks is essential for the success of their use in breeding programmes. This study evaluated the genetic diversity among okra landraces in Brazil based on morphoagronomic descriptors and AFLP markers. We studied 30 accessions of the vegetable gene bank of the Universidade Federal de Viçosa. To this end, 17 morphoagronomic descriptors and five combinations of AFLP primers were used. Genetic parameters were estimated for the quantitative traits and the accessions were grouped by Ward’s method, using the Gower’s and Jaccard’s distance measures, respectively, for the morphoagronomic and molecular data. Polymorphisms were observed for all qualitative traits, while the quantitative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Molecular markers; Phenotype; Bayesian analysis.; Recursos Genéticos.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/43426

Agrupamento de acessos e cultivares de três espécies de Brachiaria por RAPD - DOI: 10.4025/actasciagron.v30i4.5298
Provedor de dados: 1
Autores: Ambiel, Ana Claudia; UNOESTE; Guaberto, Luciana Machado; UNOESTE; Vanderlei, Talita Marques; UNOESTE; Machado Neto, Nelson Barbosa; UNOESTE.
O propósito deste trabalho foi determinar a similaridade genética entre acessos de germoplasma e cultivares comerciais de três espécies de Brachiaria (inter e intraespecífica), por meio de marcadores RAPD. Sementes foram utilizadas como fonte de DNA. 10 “primers” decâmeros foram selecionados de 120 “primers” avaliados, produzindo 107 bandas polimórficas, as quais foram utilizadas para a análise de variância molecular (Amova), o coeficiente de similaridade de Jaccard e índices de fixação gênica. 24,40% da variabilidade genética total estão contidas entre as espécies e 75,60% dentro destas, com índice de fixação gênica (FST) 0,24. No dendrograma, houve a formação de dois ramos, um formado por P. maximum e B. arrecta e o outro subdividido em três: todas...
Palavras-chave: 5.02.01.03-4    Genética e Melhoramento Florestal Brachiaria; Marcadores moleculares; Variabilidade genética; Dendrograma; Índice de fixação gênica. Brachiaria; Molecular markers; Genetic variability; Dendrogram; Genetic fixation index.
Ano: 2008
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5298

GenomicLand: Software for genome-wide association studies and genomic prediction
Provedor de dados: 1
Autores: Azevedo, Camila Ferreira; Nascimento, Moysés; Fontes, Vitor Cunha; Silva, Fabyano Fonseca e; Resende, Marcos Deon Vilela de; Cruz, Cosme Damião.
 GenomicLand is free software intended for prediction and genomic association studies based on the R software. This computational tool has an intuitive interface and supports large genomic databases, without requiring the user to use the command line. GenomicLand is available in English, can be downloaded from the Internet (https://licaeufv.wordpress.com/), and requires the Windows or Linux operating system. The software includes statistical procedures based on mixed models, Bayesian inference, dimensionality reduction and artificial intelligence. Examples of data files that can be processed by GenomicLand are available. The examples are useful to learn about the operation of the modules and statistical procedures.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Statistical analysis; Genomic analysis; Molecular markers; Biometrics.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/45361

GenomicLand: Software for genome-wide association studies and genomic prediction
Provedor de dados: 1
Autores: Azevedo, Camila Ferreira; Nascimento, Moysés; Fontes, Vitor Cunha; Silva, Fabyano Fonseca e; Resende, Marcos Deon Vilela de; Cruz, Cosme Damião.
 GenomicLand is free software intended for prediction and genomic association studies based on the R software. This computational tool has an intuitive interface and supports large genomic databases, without requiring the user to use the command line. GenomicLand is available in English, can be downloaded from the Internet (https://licaeufv.wordpress.com/), and requires the Windows or Linux operating system. The software includes statistical procedures based on mixed models, Bayesian inference, dimensionality reduction and artificial intelligence. Examples of data files that can be processed by GenomicLand are available. The examples are useful to learn about the operation of the modules and statistical procedures.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Statistical analysis; Genomic analysis; Molecular markers; Biometrics.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/45361

AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
Provedor de dados: 1
Autores: Andrade, Felipe Aranha de; Gonçalves, Leandro Simões Azeredo; Miglioranza, Édison; Ruas, Claudete de Fátima; Ruas, Paulo Mauricio; Takahashi, Lúcia Sadayo Assari.
The present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. The resulting products were denatured and subjected to capillary electrophoresis. The Jaccard distance was used to estimate the genetic distances between accessions, and the Unweighted Pair...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genética e Melhoramento Phaseolus vulgaris L.; Molecular markers; Amplified Fragment Length Polymorphism; Gene bank; Genetic diversity Recursos Genéticos Phaseolus vulgaris L.; Molecular markers; Amplified fragment length polymorphism; Gene bank.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577

The genetic diversity of strawberry (Fragaria ananassa Duch.) hybrids based on ISSR markers - doi: 10.4025/actasciagron.v35i4.16737
Provedor de dados: 1
Autores: Nunes, Claudinéia Ferreira; Empresa de Pesquisa Agropecuária de Minas Gerais; Ferreira, Juliano Lino; Empresa de Pesquisa Agropecuária de Minas Gerais; Generoso, Andressa Leal; Empresa de Pesquisa Agropecuária de Minas Gerais; Carvalho, Mário Sérgio Dias; Empresa de Pesquisa Agropecuária de Minas Gerais; Pasqual, Moacir; Universidade Federal de Lavras; Cançado, Geraldo Magela de Almeida; Empresa de Pesquisa Agropecuária de Minas Gerais.
The strawberry is an important agricultural crop in Brazil. However, most of the commercial genotypes currently in cultivation in Brazil were developed in other countries with environmental adaptations often inadequate for the regional conditions. In this work, inter-simple sequence repeat markers were used to determine the genetic variability and the loci segregation profiles of 84 strawberry hybrids obtained from a genetic breeding program at the ‘Empresa de Pesquisa Agropecuária de Minas Gerais.’ The hybrids were produced from crosses involving the following progenitors: ‘Toyonoka’ x ‘Sweet Charlie’, ‘Camino Real’ x ‘Sweet Charlie’, ‘Oso Grande’ x ‘Sweet Charlie’, ‘Oso Grande’ x ‘Toyonoka’, ‘Dover’ x ‘Oso Grande’, and ‘Camino Real’ x ‘Toyonoka’....
Tipo: Marcadores moleculares
Palavras-chave: 5.01.03.05-9 genetic dissimilarity; Pre-breeding; Molecular markers Melhoramento Vegetal genetic dissimilarity; Pre-breeding; Molecular markers.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16737

Spar genetic analysis of two invasive species of Cichla (Tucunaré) (Perciformes: Cichlidae) in the Paraná river basin - doi: 10.4025/actascibiolsci.v33i1.4855
Provedor de dados: 3
Autores: Almeida-Ferreira, Giovanna Caputo; Cesumar; Oliveira, Alessandra Valéria de; CESUMAR; Prioli, Alberto José; UEM; Prioli, Sônia Maria Alves Pinto; UEM.
The introduction of exotic species in lake ecosystems has been greatly highlighted in the literature worldwide. Since introduction may threaten diversity of native fish, the issue turns up to be of paramount importance. Ecological effects may be predation, competition, parasitism or genetic, that is, changes in the genetic pool of populations owing to the occurrence of hybrids. Although the Tucunaré fish (Cichla) is native to the Amazon region, it can be found in other hydrographic basins in which it has been introduced. RAPD molecular marker research showed that there are two species (Cichla kelberi and C. piquiti) belonging to the genus Cichla in the rivers of the Paraná basin. Different morphotypes in the region may also be due to hybridization. Current...
Palavras-chave: 2.02.00.00-5 Hybridization; Fish introduction; Molecular markers Genética Hybridization; Fish introduction; Molecular markers.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/4855

Comparative study of sulfated polysaccharides from Caulerpa spp. (Chlorophyceae). Biotechnological tool for species identification? - doi: 10.4025/actascibiolsci.v34i4.8976
Provedor de dados: 3
Autores: Rodrigues, José Ariévilo Gurgel; Rede Nordeste de Biotecnologia, Universidade Estadual do Ceará; Quinderé, Ana Luíza Gomes; Universidade Federal do Ceará; Queiroz, Ismael Nilo Lino de; Universidade Federal do Ceará; Coura, Chistiane Oliveira; Universidade Federal do Ceará; Benevides, Norma Maria Barros; Universidade Federal do Ceará.
Studies on macromolecules isolated from marine algae suggested sulfated polysaccharides (SPs) as possible molecular markers for species. We evaluated isolated and fractionated SPs from the green marine algae Caulerpa cupressoides, C. prolifera and C. racemosa collected at Pacheco  Beach, as possible taxonomic molecular indicators. Total SPs were extracted with papain in 100 mM sodium acetate buffer (pH 5.0) containing cysteine and EDTA (both 5 mM), followed by ion-exchange chromatography on DEAE-cellulose using a NaCl gradient. The obtained fractions were analyzed by 0.5% agarose gel electrophoresis. Anticoagulant assays employing normal human plasma and standard heparin (193 IU mg-1) by the activated partial thromboplastin time (APTT) test were also...
Tipo: Bioquímica comparativa de algas
Palavras-chave: 2.08.01.00-9 chlorophyta; Sulfated polysaccharides; Molecular markers; APTT test 2.08.01.00-9.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8976

The exotic jumping snail Ovachlamys fulgens (Gude, 1900) (Gastropoda: Helicarionidae) in urban areas of the Upper-Paraná Atlantic Forest
Provedor de dados: 42
Autores: BELTRAMINO,ARIEL A.; VOGLER,ROBERTO E.; RUMI,ALEJANDRA; GUZMÁN,LEILA B.; MARTÍN,STELLA M.; PESO,JUANA G.
ABSTRACT The exotic land gastropod Ovachlamys fulgens (Gude, 1900), aka the jumping snail, was registered in Eldorado city, Misiones province, constituting the first documented record of that species in Argentina-and for all of South America as well. Identities of the individuals were confirmed through morphological examination, and by a DNA sequencing of the cytochrome oxidase subunit I gene. DNA sequences for the mitochondrial 16S-rRNA and the nuclear 18S-rRNA genes were also obtained from the morphologically-confirmed specimens to gain insights into the genetic background of this species in Argentina, and as additional markers for enhancing the rapidity of identification by governmental authorities who are responsible for managing the presence of exotic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Argentina; Introduced species; Misiones province; Molecular markers; Nonnative snail.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401591

Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement
Provedor de dados: 42
Autores: SANTOS,DALILHIA N. DOS; FERREIRA,JULIANO L.; SETOTAW,TESFAHUN A.; CANÇADO,GERALDO M.A.; PASQUAL,MOACIR; LONDE,LUCIANA C.N.; SATURNINO,HELOISA M.; VENDRAME,WAGNER A.
ABSTRACT Jatropha is a potential oilseed crop, which requires mitigating factors such as the low genetic variability of the species. The solution runs through the research of Brazilian germplasm. Attention should be given to the germplasm of jatropha the north of Minas Gerais, because this is the oldest national collection and because this region may be a regions of jatropha diversity due to selection pressure arising from environmental adversities. Therefore, the objective of this study was to investigate the genetic diversity of 48 accessions of collection from Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), using SSR and ISSR markers. The results showed low genetic diversity, but some individuals stood out as J. mollissima (48), J. podagrica...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Jatropha curcas; Molecular markers; Oilseed crop.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000602363

The use of microsatellite markers in Neotropical studies of wild birds: a literature review
Provedor de dados: 42
Autores: MOURA,RENAN F.; DAWSON,DEBORAH A.; NOGUEIRA,DENISE M.
ABSTRACT Despite extensive habitat fragmentation, the Neotropical region possesses 30% of the world´s bird species. Microsatellites have remained one of the most popular genetic markers and have been used in ecological and conservation studies since the 1990’s. We conducted a literature review comparing the number of papers published from January 1990 to July 2015 that used microsatellite markers for studies of wild birds in the Neotropical region, USA and some European countries. We assigned the articles to three categories of studies: population genetics, animal behavior/kinship analysis and the development of species-specific bird microsatellite markers. We also compared the studies in the Neotropics that used heterologous versus species-specific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AVES; Cross-species amplification; Biodiversity; Ecology; Molecular markers; STR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100145

Early molecular markers predictive of dengue hemorrhagic fever
Provedor de dados: 42
Autores: Calzavara-Silva,Carlos E.; Gomes,Ana L.V.; Maia,Rita C.C.; Acioli-Santos,Bartolomeu; Gil,Laura H.V.G.; Marques Jr.,Ernesto T.A.
The management of acute dengue patients during outbreaks is a challenging problem. Most of the dengue fever cases are benign, but some cases develop into a severe and possibly lethal vasculopathy, known as dengue hemorrhagic fever. Early symptoms of dengue and hemorrhagic fever are very similar. An early differential diagnosis is needed to predict which of these two clinical presentations is crucial to proper patient care and public health management. This study evaluates the predictive potential of specific mRNA expression markers of dengue hemorrhagic fever using quantitative real-time PCR assays. Six candidate "dengue hemorrhagic fever specific signature genes" were evaluated and all showed good correlation among their transcription levels at early days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Real-time PCR; Dengue hemorrhagic fever; Molecular markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000400006

RAPD analysis revealing polymorphism in egg parasitoids of soybean stink bugs (Hemiptera: Pentatomidae)
Provedor de dados: 44
Autores: Aljanabi,Salah M.; Loiácono,Marta S.; Lourenço,Rodrigo T.; Borges,Miguel; Tigano,Myrian S.
The solitary egg parasitoids (Hymenoptera: Scelionidae) of Pentatomidae species complex are potential candidates for soybean (Glycine max L.) integrated pest management programs in Brazil. The correct identification of species and strains of these parasitoids is necessary to implement the use of biological control. Polymerase chain reaction (PCR) was used to generate random amplification of polymorphic DNA (RAPD) from male individuals of Telenomus podisi Ashmead and Trissolcus spp. collected in a soybean field in Brasília, Brazil in 1996. The analysis of 18 primers used to screen Trissolcus spp. individuals revealed different patterns related to the species analyzed. These results indicate the potential of RAPD markers to differentiate T. teretis Johnson,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Microhymenoptera parasitoids; Genetic variability; Molecular markers; RAPD.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000300009

Polymorphism of the grasshopper Schistocerca pallens (Thunberg) (Orthoptera: Acrididae) and its natural pathogen Metarhizium flavoviride Gams &amp;amp; Rozsypal (Hyphomycetes), revealed by RAPD analysis
Provedor de dados: 44
Autores: Silveira,Eni B.; Al-Janabi,Salah M.; Magalhães,Bonifácio P.; Carvalho,Luiz J.C.B.; Tigano,Myrian S.
The genetic variability of 27 individuals from two distinct populations (Northeast and Central Brazil) of the grasshopper Schistocerca pallens (Thunberg) and eight isolates of its natural pathogen, the fungus Metarhizium flavoviride Gams &amp;amp; Rozsypal, was investigated using RAPD analysis. For the grasshoppers, ten different 10-mer oligonucleotide primers of arbitrary sequence were selected for analysis, resulting in 79 scorable binary characters. The program RAPDDIP applied to S. pallens revealed nucleotide diversity of 2.3 and 2.2% for the populations from Rio Grande do Norte (Northeast Brazil) and Federal District (Central Brazil), respectively. These values indicate the presence of high genetic variability within these populations. Conversely, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Genetic variability; Entomopathogenic fungus; Biological control; Molecular markers.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000100012

Assessment of genetic diversity on a sample of cocoa accessions resistant to witches' broom disease based on RAPD and pedigree data
Provedor de dados: 53
Autores: Santos,Ronaldo Carvalho dos; Pires,José Luís; Lopes,Uilson Vanderley; Gramacho,Karina Peres G.; Flores,Acassi Batista; Bahia,Rita de Cássia S.; Ramos,Helaine C. Cristine; Corrêa,Ronan Xavier; Ahnert,Dario.
Genetic diversity in cocoa (Theobroma cacao L.) has been assessed based on morphological and molecular markers for germplasm management and breeding purposes. Pedigree data is available in cocoa but it has not been used for assessing genetic relatedness. The geneitic diversity of 30 clonal cocoa accessions resistant to witche´ broom disease, from the CEPEC series, were studied on the basis of RAPD data and pedigree information. Twenty of these accessions descend from the TSA-644 clone, originated from a cross between the Upper Amazon germplasm called Scavina-6, the main source of resistance to witches' broom disease, and IMC-67. The ten remaining clones come from different sources including Amazon and Trinitario germplasm. RAPD data was collected using 16...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coefficient of parentage; Molecular markers.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052005000300005

Comparative analysis of genetic diversity among the maize inbred lines (Zea mays L.) obtained by RAPD and SSR markers
Provedor de dados: 52
Autores: Souza,Silvia Graciele Hülse de; Carpentieri-Pípolo,Valéria; Ruas,Claudete de Fátima; Carvalho,Valdemar de Paula; Ruas,Paulo Maurício; Gerage,Antônio Carlos.
The RAPD and SSR markers were used to compare the genetic diversity among the 16 maize inbred lines. Twenty-two primers were used in the RAPD reactions, resulting in the amplification of 265 fragments, while 16 pairs of SSR primers resulted in 75 fragments. The similarity based on Dice coefficient for the RAPD ranged from 53 to 84% and for the SSR from 11 to 82%. The dendrogram obtained by the RAPD showed five groups, while dendrogram obtained by the SSR showed three groups and one isolated line. The association constructed from the markers and the principal coordinate’s analysis separated lines into two groups according to endosperm color, either orange or yellow. The RAPD were effective to validate pedigree data, while the SSR were effective to recognize...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corn; Distance genetic; Microsatellites; Molecular markers; Polymorphism; RAPD.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100022

Molecular characterization of arabica and Conilon coffee plants genotypes by SSR and ISSR markers
Provedor de dados: 52
Autores: Motta,Ludymila Brandão; Soares,Taís Cristina Bastos; Ferrão,Maria Amélia Gava; Caixeta,Eveline Teixeira; Lorenzoni,Rodrigo Monte; Souza Neto,José Dias de.
The molecular characterization of ten genotypes of the Coffea arabica plants and of seven genotypes of C. canephora having interesting features for coffee breeding programs was carried to select the parents for breeding. A total of 40 SSR and 29 ISSR primers were used. The primers generated a total of 331 (307 polymorphic and 24 monomorphic) bands. Analysis of genetic diversity presented dissimilarity intervals ranging from 0.22 to 0.44 between the Conilon genotypes, from 0.02 to 0.28 between the Arabica genotypes, and from 0.49 to 0.60 between the genotypes of the two species in the joint analysis. Four groups were formed: I = genotypes of C. arabica, II = four progenies of C. canephora, Conilon group, and one non defined C. canephora (Conilon or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea Arabica; Coffea canephora; Genetic diversity; Molecular markers.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500728

Random amplified polymorphisms between two South American subspecies of rattlesnakes (Crotalus durissus collilineatus and Crotalus durissus terrificus)
Provedor de dados: 52
Autores: Echeverrigaray,Sergio; Grazziotin,Gilberto; Grazziotin,Felipe; Agostini,Gustavo.
The present work was made to determine the suitability of RAPD analysis for the identification of specimens of two subspecies of South American rattlesnakes (Crotalus durissus terrificus and Crotalus durissus collilineatus). The 11 arbitrary primer sequence tested amplified a total of 161 bands, of which 31% were polymorphics when compared with Crotalus specimens. The combining results from different primers allowed the identification of all the specimens under analysis. Several characteristic bands of each subspecies were identified. Dendrogram, based on RAPD markers, show three groups that corresponded to the subspecies of Crotalus, and to Bothrops jararaca specimen.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rattlesnake; RAPD; Genetic variability; Molecular markers.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300014

New method for early detection of two random amplified polymorphic DNA (RAPD) groups of Staphylococcus aureus causing bovine mastitis infection in Paraná State, Brazil
Provedor de dados: 52
Autores: Gonçalves,Dicezar; Gabriel,Jane Eire; Madeira,Humberto Maciel França; Schühli,Guilherme Schnell e; Vicente,Vânia Aparecida.
The aim of this work was to develop a fast and accurate molecular approach to allow early detection of two RAPD groups of S. aureus causing bovine mastitis. Seventy five S. aureus isolates from infected animals were characterized by RAPD. Genomic fragments isolated from the unique bands present in either group were cloned and sequenced. Based on the DNA sequences, specific primers were designed to allow for the simultaneous detection of either group by multiplex PCR of S. aureus DNA isolated from clinical and subclinical bovine mastitis. Results showed that these proposed primers set could be used to detect various clinical and subclinical S. aureus isolates as well as the detection of the microorganism in bulk milk. Their use as a specific method for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Molecular markers; Subclinical mastitis; Clinical mastitis; Mastitis detection.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200014

Genetic relationships among species of the genus Bothrops based on RAPD markers
Provedor de dados: 52
Autores: Grazziotin,Felipe; Echeverrigaray,Sergio.
The genetic relationships among 11 species of Bothrops found in Brazil were determined using random amplified fragment (RAPD) information. A total of 239 amplified bands were scored using 20 aleatory primers. Most of the bands (88.7%) were polymorphic. Phylogenetic analysis of the data determined three similarity groups within Bothrops: Group I- B. alternatus, B. neuwiedi, B. cotiara, and B. jararacusu; Group II- B. insularis, B. jararaca, and B. erythromelas; and Group III- B. moojeni, B. leucurus, and B. atrox. These groups coincided with those obtained by other methods, indicating that RAPD's could be a useful tool for the evaluation of genetic relationships at the interspecific level.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops; RAPD; Molecular markers; Genetic relationship.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300005

Design and evaluation of an AFLP molecular marker for the detection of Coccidioides spp. in biological samples
Provedor de dados: 55
Autores: Reyes-Montes,María del Rocío; Frías-De-León,María Guadalupe; Victoriano-Pastelín,Isai; Acosta-Altamirano,Gustavo; Duarte-Escalante,Esperanza.
ABSTRACT At present, there is no standardized marker that is routinely used in clinical laboratories to diagnose coccidioidomycosis. Thus, the goals of this study were to obtain a sequence characterized amplified region (SCAR) marker for the identification of Coccidioides spp., evaluate its specificity and sensitivity in fungal DNA-spiked blood and sputum samples, and compare it with previously described molecular markers. Specific amplified fragment length polymorphism (AFLP) amplicons for Coccidioides immitis and Coccidioides posadasii were cloned into the vector pGEM® -T Easy vector and sequenced to develop a SCAR marker. Oligonucleotides were designed to identify Coccidioides spp. by polymerase chain reaction (PCR), and the specificity and sensitivity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; SCAR; Coccidioidomycosis; Coccidioides spp.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000500322

Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates
Provedor de dados: 55
Autores: Michelim,Lessandra; Muller,Gabriela; Zacaria,Jucimar; Delamare,Ana Paula Longaray; Costa,Sérgio Olavo Pinto da; Echeverrigaray,Sergio.
Proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (PFGE) or ribotyping, have been developed for this pathogen. However, these methods are labor intensive and time-consuming. We evaluated the discriminatory power of several PCR-based fingerprinting methods (RAPD, ISSR, ERIC-PCR, BOX-PCR and rep-PCR) for P. mirabilis clinical isolates. Typing patterns and clustering analysis indicated that RAPD, BOX-PCR and ERIC-PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proteus mirabilis; Molecular markers; Fingerprinting; PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500014

Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level
Provedor de dados: 58
Autores: Delamare,Ana Paula Longaray; Artico,Liane de Oliveira; Grazziotin,Felipe Gobbi; Echeverrigaray,Sergio; Costa,Sérgio Olavo Pinto da.
Fifteen well-defined strains of Aeromonas of thirteen species were analyzed by SDS protein electrophoretic analysis (SDS-PAGE) and random amplified polymorphic DNA analysis (RAPD). The comparison between the patterns obtained by both methods allowed differentiating all the strains. Clusters formed by the unweighted pair group method with arithmetic averages applied to protein data correlates with the genetic and biochemical information about the species. The results show that protein fingerprinting has the potential to differentiate Aeromonas species, but the low qualitative variation indicates that this technique is not efficient for the characterization of strains within a species. Conversely, RAPD fingerprinting allows the identification of strains but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aeromonas; Molecular markers; Species identification.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400016

Genetic variability of Brazilian isolates of Alternaria alternata detected by AFLP and RAPD techniques
Provedor de dados: 58
Autores: Dini-Andreote,Francisco; Pietrobon,Vivian Cristina; Andreote,Fernando Dini; Romão,Aline Silva; Spósito,Marcel Bellato; Araújo,Welington Luiz.
The Alternaria brown spot (ABS) is a disease caused in tangerine plants and its hybrids by the fungus Alternaria alternata f. sp. citri which has been found in Brazil since 2001. Due to the recent occurrence in Brazilian orchards, the epidemiology and genetic variability of this pathogen is still an issue to be addressed. Here it is presented a survey about the genetic variability of this fungus by the characterization of twenty four pathogenic isolates of A. alternata f. sp. citri from citrus plants and four endophytic isolates from mango (one Alternaria tenuissima and three Alternaria arborescens). The application of two molecular markers Random Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) had revealed the isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternaria brown spot; Molecular markers; Citrus plants; PCA analysis; Genetic variability.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300032

Nucleotide diversity of a major carotenoid biosynthetic pathway gene in wild and cultivated Solanum (Section Lycopersicon) species
Provedor de dados: 61
Autores: Araújo,Ana H.; Fonseca,Maria Esther de N.; Boiteux,Leonardo S.
A polymerase chain reaction (PCR)-based approach was employed to isolate putative alleles of the chromoplast-specific lycopene beta-cyclase (CYCB) gene from wild and cultivated tomatoes [Solanum (Section Lycopersicon)]. The objective of this work was to establish an effective PCR protocol by testing DNA samples from distinct germplasm accessions with a primer pair designed to selectively target conserved regions present in the available CYCB sequences. This PCR optimization allowed the amplification of 1219 out 1666 bp of the gene in six taxa: S. cheesmaniae, S. peruvianum, S. neorickii, S. pennellii, S. pimpinellifolium and S. lycopersicum. Sixty-three mutation sites (31 transitions, 18 transversions and 14 single base deletions/insertions) were detected...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CYCB; Lycopene; Lycopene-beta-cyclase; Nucleotide diversity; Tomatoes; Molecular markers.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000300007

Molecular Tools for Rapid and Accurate Detection of Black Truffle (Tuber melanosporum Vitt.) in Inoculated Nursery Plants and Commercial Plantations in Chile
Provedor de dados: 117
Autores: Cordero,Cecilia; Cáceres,Pablo; González,Gloria; Quiroz,Karla; Carmen,Bravo; Ramírez,Ricardo; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando.
Truffle (Tuber melanosporum Vitt.) culture is an agroforestry sector in Chile of increasing interest due to the high prices that truffles fetch in the national market and the recent evidence that its commercial production is possible in Chilean climatic and soil conditions. In this study, the efficiency of three methods of DNA extraction from a mix of 5 g of soil and roots from both nursery and field plants of Quercus ilex L. mycorrhized with T. melanosporum were evaluated, and a simple and reproducible protocol was established. Detection of T. melanosporum was performed by the technique of cleaved amplified polymorphic sequence (CAPS) from amplicons generated with the primers ADL1 (5´-GTAACGATAAAGGCCATCTATAGG-3´) and ADL3 (5´-CGTTTTTCCTGAACTCTTCATCAC-3`),...
Tipo: Journal article
Palavras-chave: Ectomycorrhizas; Molecular markers; PCR; CAPS; Ectomicorrizas; Marcadores moleculares; PCR; CAPS.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300022

Genetic Variability of the Tomato Leaf Miner (Tuta absoluta Meirick; Lepidoptera: Gelechiidae), in Tunisia, Inferred from RAPD-PCR
Provedor de dados: 117
Autores: Bettaibi,Asma; Mezghani-Khemakhem,Maha; Bouktila,Dhia; Makni,Hanem; Makni,Mohamed.
The tomato leaf miner Tuta absoluta Meyrick has invaded tomato (Solanum lycopersicum L.) crop in Tunisia since 2008 and is representing today a major threat to the production of this crop. In this study, we used the Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technology to assess the genetic variability within and among seven populations of T. absoluta, collected on tomato from different regions in Tunisia. Using five RAPD-PCR primers and 108 individuals, 140 polymorphic fragments were recorded. From 335 different RAPD phenotypes generated, 71 were redundant and 264 unique to a specific population. The genetic structure of T. absoluta was investigated using analysis of molecular variance (AMOVA), genetic distances (Fst) and...
Tipo: Journal article
Palavras-chave: Invasive species; Molecular markers; Insect populations; Gene flow.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000200008

Generalized composite interval mapping offers improved efficiency in the analysis of loci influencing non-normal continuous traits
Provedor de dados: 118
Autores: Mora,Freddy; Scapim,Carlos Alberto; Baharum,Adam; Teixeira do Amaral Júnior,Antonio.
In genetic studies, most Quantitative Trait Loci (QTL) mapping methods presuppose that the continuous trait of interest follows a normal (Gaussian) distribution. However, many economically important traits of agricultural crops have a non-normal distribution. Composite interval mapping (CIM) has been successfully applied to the detection of QTL in animal and plant breeding. In this study we report a generalized CIM (GCIM) method that permits QTL analysis of non-normally distributed variables. GCIM was based on the classic Generalized Linear Model method. We applied the GCIM method to a F2 population with co-dominant molecular markers and the existence of a QTL controlling a trait with Gamma distribution. Computer simulations indicated that the GCIM method...
Tipo: Journal article
Palavras-chave: Bioinformatics; Generalized Linear Model; Molecular markers; Quantitative Trait Loci (QTL).
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000300007

Identification and characterization of an original grapevine cultivar (Vitis vinifera) found in Chile
Provedor de dados: 118
Autores: Gonzalez,Alvaro S; Massera,Franco; Moscoso,Daniel; Hinrichsen,Patricio; Montenegro,Gloria; Laucou,Valerie; Lacombe,Thierry; Boursiquot,Jean-Michel; Pszczólkowski,Philippo.
Currently, many monovarietal vineyards present a minority of mixed cultivars. Some of these cultivars are unknown and could represent an opportunity to discover new and potentially useful genotypes both for research and production purposes. In a 'Carmenère' vineyard planted in 1994 in Palmilla (Colchagua Valley, Chile), a minority presence of other cultivars was found. The present work covers for the first time the identification and characterization of one of these cultivars, which presented a very poor fruit set. Through classic ampelography, it was not possible to associate the studied cultivar with any known cultivar in Chile. However, through a simple sequence repeats (SSR) comparison against the database of the INRA Germplasm Repository "Domaine de...
Tipo: Journal article
Palavras-chave: Ampelography; Chile; Coulure; Molecular markers; Poor fruit set; SSR; Triploid.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000200016

Genetic variability in maize and teosinte populations estimated by microsatellites markers
Provedor de dados: 65
Autores: Terra,Tatiana de Freitas; Wiethölter,Paula; Almeida,Cícero Carlos de Souza; Silva,Sérgio Delmar dos Anjos e; Bered,Fernanda; Sereno,Maria Jane Cruz de Melo; Barbosa Neto,José Fernandes.
Wild species are important sources of genetic variability and may be exploited by breeding programs. Crosses between teosinte and maize occur freely and teosinte serves as genetic source of agronomic traits for introduction in maize. The objective of this study was to estimate genetic variability among and within maize and teosinte populations (Zea mays mexicana). Two sweet maize populations (BR400 and BR402), two common maize populations (Suwan and Pampa) and one teosinte population were analyzed using microsatellites markers. Results indicated that 64,5% of the variation was detected within the populations, suggesting the possibility of obtaining genetic progress by selection within each population. The analysis with 25 microsatellites loci enabled the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays mays; Zea mays mexicana; Genetic diversity; Flooding tolerance; Molecular markers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000200003

Cross-amplification of heterologous microsatellite markers in Piracanjuba
Provedor de dados: 65
Autores: Castro,Pedro Luiz de; Ribeiro,Ricardo Pereira; Santos,Silvio Carlos Alves dos; Goes,Elenice Souza dos Reis; Souza,Felipe Pinheiro de; Poveda-Parra,Angela Rocio; Vargas,Lauro; Urrea-Rojas,Angela Maria; Lopera-Barrero,Nelson Mauricio.
ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brycon orbignyanus; Conservation; Molecular markers; Heterologous primers.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200402

Biological insect control using Metarhizium anisopliae: morphological, molecular, and ecological aspects
Provedor de dados: 65
Autores: Tiago,Patricia Vieira; Oliveira,Neiva Tinti de; Lima,Elza Áurea de Luna Alves.
Microbial control of insects is based on the rational use of pathogens to maintain environmentally balanced pest population levels, and Metarhizium anisopliae has been the most studied and most utilized fungal species for that purpose. The natural genetic variability of entomopathogenic fungi is considered one of the principal advantages of microbial insect control. The inter- and intraspecific variability and the genetic diversity and population structures of Metarhizium and other entomopathogenic fungi have been examined using ITS-RFLP, ISSR, and ISSP molecular markers. The persistence of M. anisopliae in the soil and its possible effects on the structures of resident microbial communities must be considered when selecting isolates for biological insect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Metarhizium anisopliae; Molecular markers.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000400012

Relationship between heterosis and genetic divergence for phosphorus use efficiency and its components in tropical maize
Provedor de dados: 65
Autores: Caixeta,Débora Santos; Fritsche-Neto,Roberto; Batista,Lorena Guimarães; Carvalho,Humberto Fanelli; DoVale,Júlio César; Lanes,Éder Cristian Malta de; Miranda,Glauco Vieira.
The objective of this study was to determine the relationship between heterosis and genetic divergence for phosphorus use efficiency (PUE) in tropical maize. It was used two groups of genitors, each consisting of seven lines, contrasting with each other in the nitrogen and phosphorus use efficiency. It was obtained 41 hybrid combinations between these groups, which were evaluated in low phosphorus. Randomized complete block design with two replications was used. For obtaining the components of variance and the breeding values were used REML/BLUP method. In the genotyping of the parental lines were used 80 microsatellite markers. Through the correlation between genetic distance obtained by the markers and specific combining ability it was not possible to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dissimilarity; Abiotic stress; Molecular markers; Combining ability.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100011

Transferability of heterologous microsatellite primers in Brycon gouldingi
Provedor de dados: 65
Autores: Souza,Felipe Pinheiro de; Lima,Ed Christian Suzuki de; Leite,Natalia Gonçalves; Urrea-Rojas,Angela Maria; Yamachita,Andrei Lincoln; Pandolfi,Victor César Freitas; Lopera-Barrero,Nelson Mauricio.
ABSTRACT:  Brycon gouldingi is a species of neotropical fish of socioeconomic and environmental importance in the Tocantins-Araguaia Basin. Genetic studies on this species are still limited, making it difficult to evaluate the population structure and genetic diversity in natural and captive stocks. Here, we aimed to evaluate the transferability of heterologous microsatellite primers in B. gouldingi. A total of 30 primers for eight species were evaluated: Brycon hilarii, Brycon opalinus, Brycon cephalus, Brycon orbignyanus, Prochilodus lineatus, Prochilodus argenteus, Piaractus mesopotamicus, and Colossoma macropomum. The primers that showed the best amplification patterns were applied to 20 specimens of B. gouldingi, and their genetic parameters were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Cross-amplification; Molecular markers; SSR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100406

Identification of Pm24, Pm35 and Pm37 in thirteen Egyptian bread wheat cultivars using SSR markers
Provedor de dados: 64
Autores: Emara,Hassan Mahmoud; Omar,Ayman Feisal; El-Shamy,Moustafa Mahmoud; Mohamed,Mona Elsaid.
ABSTRACT Powdery mildew of wheat (Triticum spp.) caused by Blumeria graminis f.sp. tritici (DC) E.O. Speer Em. Marchal is one of the most important bread wheat diseases in Egypt. All the Egyptian common bread wheat cultivars are susceptible to that disease at seedling and adult stages. Breeding of resistant cultivars is the most economical and environmentally safe method to eliminate the disease and reduce crop losses. Combinations of two or more effective resistance genes may lead to better, more durable resistance to that disease. Eight Pm genes i.e. Pm2, Pm6, Pm12, Pm16, Pm24, Pm35, Pm36 and Pm37 out of 21 powdery mildew monogenic wheat lines (Pm) were resistant to 42 individual isolates of powdery mildew collected from different governorates in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Powdery mildew; Genetic control; DNA; Molecular markers.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542016000300279

Genetic diversity in Australian Cedar genotypes selected by mixed models
Provedor de dados: 64
Autores: Tavares,Rulfe; Viana,Alexandre Pio; Barroso,Deborah Guerra; Amaral Júnior,Antonio Teixeira do.
The increasing demand for raw material for multiple uses of forest products and by-products has attracted the interest for fast growing species, such as the Australian Cedar (Toona ciliata), which presents high productive and economic potential. The present work aimed at estimating genetic diversity by DNA markers and morphological traits supported for the mixed models. The following traits were measured and genotypes were sampled randomly in different areas: diameter at breast height, height, cylindrical volume, diameter, distance between nodes and crown diameter. Twelve RAPD primers were used and generated a total of 91 marks, 82 of which were polymorphic. The high percentage of polymorphic markers, 90.10%, demonstrated that discrimination in this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Genetic values; Woody plant.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000200005

Molecular characterization of elephantgrass accessions through RAPD markers
Provedor de dados: 64
Autores: Passos,Leônidas Paixão; Machado,Marco Antonio; Vidigal,Maria Coletta; Campos,Ana Lúcia.
Elephantgrass pastures are limited by yield variations and reductions in forage quality and availability, thus making the search for genotypes with reduced seasonality a major concern. In order to verify the extent of genetic variability among contrasting cultivars, ten elephantgrass accessions were analyzed through DNA amplification by RAPD technique. A total of 160 DNA bands were generated with the use of 44 random primers and 23% of these bands were monomorphic for all accessions. Gel-obtained binary data (1 for presence and 0 for absence) were used for generating a genetic distance matrix, which was utilized in a UPGMA grouping analysis. Elephantgrass cultivars Cameroon and Vruckwona were the accessions mostly divergent from the others, with an average...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Elephantgrass; RAPD; DNA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000300009

Performance of the double-cross tomato hybrids from a partial diallel
Provedor de dados: 64
Autores: Matos,Rafael de; Resende,Juliano Tadeu Vilela de; Zeist,André Ricardo; Corte,Lígia Erpen-Dalla; Da-Silva,Paulo Roberto; Zeffa,Douglas Mariani.
ABSTRACT Knowledge of the combining ability of genotypes, genetic diversity, and heterosis play the paramount role in obtaining double-cross hybrids with high productivity. This study was aimed at evaluating these parameters in tomato genotypes using partial diallel crosses among the commercial single-cross hybrids, which verified the possibility of using double-cross hybrids for commercial purposes. Tomato genotypes included 15 double-cross hybrids, their genitors, and two commercial genotypes as checks. Of the fifteen double-cross hybrids of tomato, two crosses exhibited superiority, such as Aguamiel × Compack and Dominador × Compack, mainly for the total and commercial fruit yields. The phenotypic expression of the trait is controlled by the genes with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum lycopersicum; ISSR; Molecular markers; Plant breeding; Heterosis.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100205

Characterization of trees, fruits and genetic diversity in natural populations of mangaba
Provedor de dados: 64
Autores: Silva,Ana Veruska Cruz da; Amorim,Julie Anne Espíndola; Vitória,Marina Ferreira da; Ledo,Ana da Silva; Rabbani,Allivia Rouse Carregosa.
ABSTRACT The state of Sergipe is the largest mangaba producer, which is a fruit native to Brazil, and has cultural, social and economic importance in its area of occurrence. It is an endangered species due to human actions, and despite its economic potential, there are still no commercial plantations. The study was carried out in order to characterize trees, fruits and the genetic diversity of natural populations of mangaba in Sergipe, Brazil. Fruits from Abaís Beach/Estância (AB) presented, on average, twice the vitamin C content (414.81 mg of vit. C/100g), when compared with the others. The use of ISSR primers was efficient in estimating the genetic similarity of populations. The primers clustered the populations of mangaba according to their origin,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hancornia speciosa Gomes; Postharvest; Molecular markers; Variability.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542017000300255