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Molecular biology of the kallikrein-kinin system: from structure to function
Provedor de dados: 56
Autores: Pesquero,J.B.; Bader,M.
The participation of the kallikrein-kinin system, comprising the serine proteases kallikreins, the protein substrates kininogens and the effective peptides kinins, in some pathological processes like hypertension and cardiovascular diseases is still a matter of controversy. The use of different experimental set-ups in concert with the development of potent and specific inhibitors and antagonists for the system has highlighted its importance but the results still lack conclusivity. Over the last few years, transgenic and gene-targeting technologies associated with molecular biology tools have provided specific information about the elusive role of the kallikrein-kinin system in the control of blood pressure and electrolyte homeostasis. cDNA and genomic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kallikrein; Kinins; Bradykinin; Molecular biology; Transgenics.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900013

Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera
Provedor de dados: 56
Autores: Santana,A.N.C.; Trindade-Filho,E.M.; Cunha,R.B.; Sousa,M.V.; Cavalheiro,E.A.; Carvalho,K.M.
In this study, the behavioral and electroencephalographic (EEG) analysis of seizures induced by the intrahippocampal injection in rats of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera, was determined. The first alterations occurred during microinjection of granulitoxin (8 µg) into the dorsal hippocampus and consisted of seizure activity that began in the hippocampus and spread rapidly to the occipital cortex. This activity lasted 20-30 s, and during this period the rats presented immobility. During the first 40-50 min after its administration, three to four other similar short EEG seizure periods occurred and the rats presented the following behavioral alterations: akinesia, facial automatisms, head tremor, salivation,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bunodosoma granulifera; Granulitoxin; Sea anemones; Toxic peptides; Seizure; Electroencephalographic assay.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600016

APOA1/C3/A4 gene cluster variability and lipid levels in Brazilian children
Provedor de dados: 56
Autores: França,E. de; Alves,J.G.B.; Hutz,M.H.
Genetic studies have suggested that polymorphisms of genes coding for apolipoproteins are significant determinants of serum lipoprotein and lipid levels in adults. However, only a few studies have investigated the association of these polymorphisms in children. Therefore, in the present investigation we studied the distribution of APOA1 -75 G&gt;A, +83 C&gt;T, APOC3 -482 C&gt;T, -455 T&gt;C and 3238 C&gt;G, and APOA4 Q360H and T347S polymorphisms and their influence on plasma lipoprotein levels in children from a Brazilian northeastern admixed population. The seven polymorphic sites were genotyped in 414 children aged 5 to 15 years (mean 8.9 ± 2.9). The genotypes of the seven polymorphic sites were assessed by PCR-RFLP methods. The frequencies of the less...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Apolipoproteins; Gene variants; Lipid levels; Children.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400006

Prevalence of metabolic syndrome and its association with educational inequalities among Brazilian adults: a population-based study
Provedor de dados: 56
Autores: Gronner,M.F.; Bosi,P.L.; Carvalho,A.M.; Casale,G.; Contrera,D.; Pereira,M.A.; Diogo,T.M.; Torquato,M.T.C.G.; Souza,G.M.D.; Oishi,J.; Leal,A.M.O.
The present study estimated the prevalence of metabolic syndrome (MS) according to the criteria established by the National Cholesterol Education Program-Adult Treatment Panel III (NCEP-ATPIII) and the International Diabetes Federation (IDF) and analyzed the contribution of social factors in an adult urban population in the Southeastern region of Brazil. The sample plan was based on multistage probability sampling according to family head income and educational level. A random sample of 1116 subjects aged 30 to 79 years was studied. Participants answered a questionnaire about socio-demographic variables and medical history. Fasting capillary glucose (FCG), total cholesterol, high-density lipoprotein cholesterol (HDL-C), and triglycerides were determined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic syndrome; Prevalence; Brazil; Diabetes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700016

Zinc and water intake in rats: investigation of adrenergic and opiatergic central mechanisms
Provedor de dados: 56
Autores: Fregoneze,J.B.; Luz,C.P.; Castro,L.; Oliveira,P.; Lima,A.K.S.; Souza,F.; Maldonado,I.; Macêdo,D.F.; Ferreira,M.G.; Bandeira,I.P.V.; Rocha Jr.,M.A.; Carvalho,F.L.Q.; De-Castro-e-Silva,E.
We have demonstrated that central administration of zinc in minute amounts induces a significant antidipsogenic action in dehydrated rats as well as in rats under central cholinergic and angiotensinergic stimulation. Here we show that acute third ventricle injections of zinc also block water intake induced by central ß-adrenergic stimulation in Wistar rats (190-250 g). Central inhibition of opioid pathways by naloxone reverses the zinc-induced antidipsogenic effect in dehydrated rats. After 120 min, rats receiving third ventricle injections of isoproterenol (160 nmol/rat) exhibited a significant increase in water intake (5.78 ± 0.54 ml/100 g body weight) compared to saline-treated controls (0.15 ± 0.07 ml/100 g body weight). Pretreatment with zinc (3.0,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Zinc; Isoproterenol; Opioids; Thirst; Naloxone; Rats.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000007

Mesenchymal stem cells from patients with chronic myeloid leukemia do not express BCR-ABL and have absence of chimerism after allogeneic bone marrow transplant
Provedor de dados: 56
Autores: Carrara,R.C.V.; Orellana,M.D.; Fontes,A.M.; Palma,P.V.B.; Kashima,S.; Mendes,M.R.; Coutinho,M.A.; Voltarelli,J.C.; Covas,D.T.
Bone marrow is a heterogeneous cell population which includes hematopoietic and mesenchymal progenitor cells. Dysregulated hematopoiesis occurs in chronic myelogenous leukemia (CML), being caused at least in part by abnormalities in the hematopoietic progenitors. However, the role of mesenchymal stem cells (MSCs) in CML has not been well characterized. The objectives of the present study were to observe the biological characteristics of MSCs from CML patients and to determine if MSCs originate in part from donors in CML patients after bone marrow transplantation (BMT). We analyzed MSCs from 5 untreated patients and from 3 CML patients after sex-mismatched allogeneic BMT. Flow cytometry analysis revealed the typical MSC phenotype and in vitro assays showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem cells; Chimerism; Allogeneic hematopoietic stem cell transplantation; Chronic myeloid leukemia; Bcr-abl gene.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100008

Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800)
Provedor de dados: 56
Autores: Artico,L.O.; Bianchini,A.; Grubel,K.S.; Monteiro,D.S.; Estima,S.C.; de Oliveira,L.R.; Bonatto,S.L.; Marins,L.F.
The South American sea lion, Otaria flavescens, is widely distributed along the Pacific and Atlantic coasts of South America. However, along the Brazilian coast, there are only two nonbreeding sites for the species (Refúgio de Vida Silvestre da Ilha dos Lobos and Refúgio de Vida Silvestre do Molhe Leste da Barra do Rio Grande), both in Southern Brazil. In this region, the species is continuously under the effect of anthropic activities, mainly those related to environmental contamination with organic and inorganic chemicals and fishery interactions. This paper reports, for the first time, the genetic diversity of O. flavescens found along the Southern Brazilian coast. A 287-bp fragment of the mitochondrial DNA control region (D-loop) was analyzed. Seven...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Otaria flavescens; MtDNA; D-loop; Genetic variability; New haplotypes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900002

Reference values for lung function tests: III. Carbon monoxide diffusing capacity (transfer factor)
Provedor de dados: 56
Autores: Neder,J.A.; Andreoni,S.; Peres,C.; Nery,L.E.
Carbon monoxide diffusing capacity (DLCO) or transfer factor (TLCO) is a particularly useful test of the appropriateness of gas exchange across the lung alveolocapillary membrane. With the purpose of establishing predictive equations for DLCO using a non-smoking sample of the adult Brazilian population, we prospectively evaluated 100 subjects (50 males and 50 females aged 20 to 80 years), randomly selected from more than 8,000 individuals. Gender-specific linear prediction equations were developed by multiple regression analysis with single breath (SB) absolute and volume-corrected (VA) DLCO values as dependent variables. In the prediction equations, age (years) and height (cm) had opposite effects on DLCOSB (ml min-1 mmHg-1), independent of gender (-0.13...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung diffusing capacity; Pulmonary function tests; Maximal oxygen consumption.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600008

Risk factors for pulmonary hypertension in patients receiving maintenance peritoneal dialysis
Provedor de dados: 56
Autores: Zeng,Y.; Yang,D.D.; Feng,S.; Shen,H.Y.; Wang,Z.; Jiang,S.; Shi,Y.B.; Fu,J.X.
We investigated the risk factors for pulmonary hypertension (PH) in patients receiving maintenance peritoneal dialysis (MPD). A group of 180 end-stage renal disease patients (124 men and 56 women; mean age: 56.43±8.36) were enrolled in our study, which was conducted between January 2009 and June 2014. All of the patients received MPD treatment in the Dialysis Center of the Second Affiliated Hospital of Soochow University. Clinical data, laboratory indices, and echocardiographic data from these patients were collected, and follow-ups were scheduled bi-monthly. The incidence and relevant risk factors of PH were analyzed. The differences in measurement data were compared by t-test and enumeration data were compared with the χ2 test. Among the 180 patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maintenance peritoneal dialysis; Pulmonary hypertension; End-stage renal disease; Internal arteriovenous fistula; Hypertensive nephropathy; Ejection fraction.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300703

p16INK4 expression in precursor lesions of squamous cell cervical cancer related to the presence of HPV-DNA
Provedor de dados: 56
Autores: Godoy,A.E.G.; Mandelli,J.; Oliveira,F.H.; Calegari,S.; Moura,L.B.; Serafini,E.P.
The purpose of the present study was to identify the expression of p16INK4 in cervical cancer precursor lesions by immunohistochemistry and to correlate it with lesion grade and presence of human papillomavirus (HPV) infection. Cervical specimens from 144 women seen consecutively at the gynecology outpatient clinic of our institution from December 2003 to May 2005 were analyzed by cytopathology, histopathology, polymerase chain reaction for HPV-DNA, and p16INK4 immunostaining. Histologically normal biopsies, HPV-DNA negative by polymerase chain reaction, were used as control. HPV-DNA prevalence, including the control group, was 68.1% and the prevalence of p16INK4 expression was 55.0%. The percentage of cells stained by p16INK4 ranged from 10 to 100%, both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P16INK4; Cervical cancer; Human papillomavirus; Immunohistochemistry.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700006

Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons
Provedor de dados: 56
Autores: Santos,D.V.V.; Costa,K.M.; Vaz,M.C.G.; Silva Filho,M. Da.
The cortical layer 1 contains mainly small interneurons, which have traditionally been classified according to their axonal morphology. The dendritic morphology of these cells, however, has received little attention and remains ill defined. Very little is known about how the dendritic morphology and spatial distribution of these cells may relate to functional neuronal properties. We used biocytin labeling and whole cell patch clamp recordings, associated with digital reconstruction and quantitative morphological analysis, to assess correlations between dendritic morphology, spatial distribution and membrane properties of rat layer 1 neurons. A total of 106 cells were recorded, labeled and subjected to morphological analysis. Based on the quantitative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biocytin; Patch-clamp; Dendritic morphology; Layer 1; Neuronal variability.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200016

Transient elastography and APRI score: looking at false positives and false negatives. Diagnostic performance and association to fibrosis staging in chronic hepatitis C
Provedor de dados: 56
Autores: Mendes,L.C.; Ferreira,P.A.; Miotto,N.; Zanaga,L.; Gonçales,E.; Lazarini,M.S.; Gonçales Júnior,F.L.; Stucchi,R.S.B.; Vigani,A.G.
Although long regarded as the gold standard for liver fibrosis staging in chronic hepatitis C (CHC), liver biopsy (LB) implies both the risk of an invasive procedure and significant variability. The aim of this study was to evaluate the diagnostic performance for transient elastography (TE) and aspartate aminotransferase to platelet index (APRI) used alone and in combination compared to liver biopsy and to analyze false positive/negative results. Patients with CHC, and no previous clinical diagnosis of cirrhosis were enrolled to undergo liver biopsy, TE and APRI. A total of 182 adult patients with a median age of 55 years and median body mass index of 26.71 kg/m2 were analyzed. On LB, 56% of patients had significant levels of fibrosis (METAVIR F≥2) and 28%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver biopsy; Noninvasive tests; APRI; Transient elastography; Accuracy; False results.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900702

Association of Alport's syndrome with HLA-DR2 antigen in a group of unrelated patients
Provedor de dados: 56
Autores: Donadi,E.A.; Voltarelli,J.C.; Paula-Santos,C.M.; Kimachi,T.; Ferraz,A.S.
A few family studies have evaluated HLA antigens in Alport's syndrome; however, there are no large population studies. In the present report, we studied 40 unrelated white patients with Alport's syndrome seen at the Unit of Renal Transplantation, Faculty of Medicine of Ribeirão Preto, São Paulo, Brazil. HLA-A, -B, -DR and -DQ antigens were typed using a complement-dependent microlymphocytotoxicity assay. A control white population (N = 403) from the same geographical area was also typed for HLA antigens. Although the frequencies of HLA-A and -B antigens of patients were not statistically different from controls, the frequency of HLA-DR2 antigen observed in patients (65%) was significantly increased in relation to controls (26%; P&amp;lt;0.001). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA antigens; Alport's syndrome; MHC; Immunogenetics.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400010

Induction of Fos protein immunoreactivity by spinal cord contusion
Provedor de dados: 56
Autores: Del-Bel,E.A.; Borges,C.A.G.; Defino,H.L.A.; Guimarães,F.S.
The objective of the present study was to identify neurons in the central nervous system that respond to spinal contusion injury in the rat by monitoring the expression of the nuclear protein encoded by the c-fos gene, an activity-dependent gene, in spinal cord and brainstem regions. Rats were anesthetized with urethane and the injury was produced by dropping a 5-g weight from 20.0 cm onto the exposed dura at the T10-L1 vertebral level (contusion group). The spinal cord was exposed but not lesioned in anesthetized control animals (laminectomy group); intact animals were also subjected to anesthesia (intact control). Behavioral alterations were analyzed by Tarlov/Bohlman scores, 2 h after the procedures and the animals were then perfused for...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fos immunohistochemistry; Intermediolateral column neurons; Reticular formation; Area postrema; Dorsal horn; Solitary tract nuclei; Laminectomy; Spinal cord injury.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500006

Psychometric properties of the Portuguese version of the State-Trait Anxiety Inventory applied to college students: factor analysis and relation to the Beck Depression Inventory
Provedor de dados: 56
Autores: Andrade,L.; Gorenstein,C.; Vieira Filho,A.H.; Tung,T.C.; Artes,R.
The psychometric properties of the Portuguese version of the trait form of the State-Trait Anxiety Inventory (STAI-T) and its relation to the Beck Depression Inventory (BDI) were evaluated in a large Brazilian college student sample containing 845 women and 235 men. STAI-T scores tended to be higher for women, singles, those who work, and subjects under 30 years. Factor analysis of the STAI-T for total sample and by gender yielded two factors: the first representing a mood dimension and the second being related to worrying or cognitive aspects of anxiety. In order to study the relation between anxiety and depression measures, factor analysis of the combination of the 21 BDI items and the 20 STAI-T items was also carried out. The analysis resulted in two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Strait-Trait Anxiety Inventory; Factor analysis; Psychometric properties; Beck Depression Inventory; Students.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300011

Normal expression of IFN-gammaR in four patients with uncommon mycobacterial infection phenotypes
Provedor de dados: 56
Autores: Rugeles,M.T.; Rincón,B.; Rugeles,C.; Montoya,C.J.; Hernández,M.; Estrada,C.; Olivares,M.M.; Patiño,P.J.
Several primary immunodeficiency diseases affecting the interleukin 12/interferon gamma (IFN-gamma) pathway have been identified, most of them characterized by recurrent and protracted infections produced by intracellular microorganisms, particularly by several species of mycobacteria. In the present study we analyzed the expression of IFN-gamma receptor (IFN-gammaR) and signal transducer and activator of transcription 1 (STAT-1) in 4 children with Mycobacterium tuberculosis infection of uncommon clinical presentation. These molecules were evaluated by flow cytometry and Western blotting in B cells transformed with Epstein-Barr virus and mutations were scanned by single-strand conformational polymorphisms and DNA sequencing. The expression of IFN-gammaR1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterial disease; IFN-gamma; IFN-gamma receptor; STAT-1; SSCP-PCR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900010

Schizandrin A exerts anti-tumor effects on A375 cells by down-regulating H19
Provedor de dados: 56
Autores: Bi,Yiming; Fu,Yan; Wang,Shuyan; Chen,Xingxiu; Cai,Xiaoping.
Malignant melanoma (MM) is one of the malignant tumors with highly metastatic and aggressive biological actions. Schizandrin A (SchA) is a bioactive lignin compound with strong anti-oxidant and anti-aging properties, which is stable at room temperature and is often stored in a cool dry place. Hence, we investigated the effects of SchA on MM cell line A375 and its underlying mechanism. A375 cells were used to construct an in vitro MM cell model. Cell viability, proliferation, apoptosis, and migration were detected by Cell Counting Kit-8, BrdU assay, flow cytometry, and transwell two-chamber assay, respectively. The cell cycle-related protein cyclin D1 and cell apoptotic proteins (Bcl-2, Bax, cleaved-caspase-3, and cleaved-caspase-9) were analyzed by western...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malignant melanoma; Schizandrin A; H19; PI3K/AKT.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000610

Bone mineral density of the lumbar spine of Brazilian children and adolescents aged 6 to 14 years
Provedor de dados: 56
Autores: Fonseca,A.S.M.; Szejnfeld,V.L.; Terreri,M.T.; Goldenberg,J.; Ferraz,M.B.; Hilário,M.O.E.
The authors performed a study of bone mass in eutrophic Brazilian children and adolescents using dual-energy X-ray absorptiometry (DXA) in order to obtain curves for bone mineral content (BMC) and bone mineral density (BMD) by chronological age and correlate these values with weight and height. Healthy Caucasian children and adolescents, 120 boys and 135 girls, 6 to 14 years of age, residents of São Paulo, Brazil, were selected from the Pediatric Department outpatient clinic of Hospital São Paulo (Universidade Federal de São Paulo). BMC, BMD and the area of the vertebral body of the L2-L4 segment were obtained by DXA. BMC and BMD for the lumbar spine (L2-L4) presented a progressive increase between 6 and 14 years of age in both sexes, with a distribution...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone mineral density; Lumbar spine densitometry; Children; Adolescents.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300008

Effects of high- and moderate-intensity exercise on central hemodynamic and oxygen uptake recovery kinetics in CHF-COPD overlap
Provedor de dados: 56
Autores: Mazzuco,A.; Souza,A.S.; Medeiros,W.M.; Sperandio,P.A.; Alencar,M.C.N.; Arbex,F.F.; Neder,J.A.; Borghi-Silva,A.
The oxygen uptake (V˙O2) kinetics during onset of and recovery from exercise have been shown to provide valuable parameters regarding functional capacity of both chronic obstructive pulmonary disease (COPD) and chronic heart failure (CHF) patients. To investigate the influence of comorbidity of COPD in patients with CHF with reduced ejection fraction on recovery from submaximal exercise, 9 CHF-COPD male patients and 10 age-, gender-, and left ventricle ejection fraction (LVEF)-matched CHF patients underwent constant-load exercise tests (CLET) at moderate and high loads. The V˙O2, heart rate (HR), and cardiac output (CO) recovery kinetics were determined for the monoexponential relationship between these variables and time. Within-group analysis showed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic heart failure; Chronic obstructive pulmonary disease; Oxygen uptake; Heart rate; Recovery kinetics; Exercise test.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300602

Purinergic signalling: past, present and future
Provedor de dados: 56
Autores: Burnstock,G.
The discovery of non-adrenergic, non-cholinergic neurotransmission in the gut and bladder in the early 1960's is described as well as the identification of adenosine 5'-triphosphate (ATP) as a transmitter in these nerves in the early 1970's. The concept of purinergic cotransmission was formulated in 1976 and it is now recognized that ATP is a cotransmitter in all nerves in the peripheral and central nervous systems. Two families of receptors to purines were recognized in 1978, P1 (adenosine) receptors and P2 receptors sensitive to ATP and adenosine diphosphate (ADP). Cloning of these receptors in the early 1990's was a turning point in the acceptance of the purinergic signalling hypothesis and there are currently 4 subtypes of P1 receptors, 7 subtypes of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Purinoceptors; P1; P2X; P2Y ATP release and breakdown Purinergic neuropathology Pain Neurodegenerative diseases.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100002

Distribution of AMPA-type glutamate receptor subunits in the chick visual system
Provedor de dados: 56
Several glutamate receptor (GluR) subunits have been characterized during the past few years. In the present study, subunit-specific antisera were used to determine the distribution of the AMPA-type glutamate receptor subunits GluR1-4 in retinorecipient areas of the chick brain. Six white leghorn chicks (Gallus gallus, 7-15 days old, unknown sex) were deeply anesthetized and perfused with 4% buffered paraformaldehyde and brain sections were stained using immunoperoxidase techniques. The AMPA-type glutamate receptor subunits GluR1, GluR2/3 and GluR4 were present in several retinorecipient areas, with varying degrees of colocalization. For example, perikarya in layers 2, 3, and 5 of the optic tectum contained GluR1, whereas GluR2/3 subunits appeared mainly...
Palavras-chave: Glutamate; Receptors; Neurotransmitters; Visual system; Visual pathways.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100012

Women with recurrent spontaneous abortion have decreased 25(OH) vitamin D and VDR at the fetal-maternal interface
Provedor de dados: 56
Autores: Li,N.; Wu,H.M.; Hang,F.; Zhang,Y.S.; Li,M.J.
Immunological mechanisms have been proposed to underlie the pathogenesis of recurrent spontaneous abortion (RSA). Vitamin D has a potent immunomodulatory effect, which may affect pregnancy outcome. The objective of this study was to investigate 25-hydroxyvitamin D [25(OH) D] concentration and vitamin D receptor (VDR) expression in the decidual tissues of RSA patients. Thirty women with RSA (RSA group) and thirty women undergoing elective abortion (control group) were recruited during 2016 from gynecology outpatient clinics. We measured 25(OH) D, interleukin (IL)-17, IL-23, transforming growth factor β (TGF-β), VDR and 1-α-hydroxylase (CYP27B1) in decidual tissues collected during the abortion procedure. In the RSA group, 25(OH) D and TGF-β were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recurrent spontaneous abortion (RSA); Treg/Th17 cells; Maternal-fetal interface; 25(OH) D; Vitamin D metabolism.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100606

Long non-coding RNA CHCHD4P4 promotes epithelial-mesenchymal transition and inhibits cell proliferation in calcium oxalate-induced kidney damage
Provedor de dados: 56
Autores: Zhang,C.; Yuan,J.; Hu,H.; Chen,W.; Liu,M.; Zhang,J.; Sun,S.; Guo,Z.
Kidney stone disease is a major cause of chronic renal insufficiency. The role of long non-coding RNAs (lncRNAs) in calcium oxalate-induced kidney damage is unclear. Therefore, we aimed to explore the roles of lncRNAs in glyoxylate-exposed and healthy mouse kidneys using microarray technology and bioinformatics analyses. A total 376 mouse lncRNAs were differentially expressed between the two groups. Using BLAST, 15 lncRNA homologs, including AU015836 and CHCHD4P4, were identified in mice and humans. The AU015836 expression in mice exposed to glyoxylate and the CHCHD4P4 expression in human proximal tubular epithelial (HK-2) cells exposed to calcium oxalate monohydrate were analyzed, and both lncRNAs were found to be upregulated in response to calcium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney calculi; Long non-coding RNAs; Fibrosis; Cell proliferation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100610

Application of various antioxidants in the treatment of influenza
Provedor de dados: 56
Autores: Dolganova,A.; Sharonov,B.P.
We determined the effect of the antioxidants superoxide dismutase, desferrioxamine and allopurinol on the survival of male CBA mice infected intranasally with 2-5 LD50 lung influenza virus A/Aichi/2/68. Survival for at least 20 days was observed for 45% of the mice that received 1000 U/day superoxide dismutase prepared from red blood cells on days 5, 6, 7 and 8 after infection, and 75% survival was observed for mice that received the same dose on days 4, 5, 6, 7 and 8. Desferrioxamine, 25 mg/kg per day and 100 mg/kg per day injected subcutaneously, resulted in survival rates of 5 and 0%, respectively, compared to 10% survival observed for saline-injected controls. Allopurinol at doses of 5 to 50 mg/kg per day had no effect on mouse survival. These data...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Free radicals; Influenza; Antioxidant; Desferrioxamine; Superoxide dismutase; Allopurinol.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100012

Histopathological analysis of rat mesentery as a method for evaluating neutrophil migration: differential effects of dexamethasone and pertussis toxin
Provedor de dados: 56
Autores: Brito,G.A.C.; Falcão,J.L.A.A.; Saraiva,S.N.R.; Lima,A.A.M.; Flores,C.A.; Ribeiro,R.A.
In the present study, histopathological analysis of rat mesentery was used to quantify the effect of two anti-inflammatory agents, dexamethasone (Dex) and pertussis toxin (Ptx), on leukocyte migration. The intravenous injection of Dex (1 mg/kg) and Ptx (1,200 ng) 1 h prior to the intraperitoneal injection of the inflammatory stimuli lipopolysaccharide (LPS) or formyl-methionyl-leucyl-phenylalanine (fMLP) significantly reduced the neutrophil diapedesis (LPS: Ptx = 0.86 ± 0.19 and Dex = 0.35 ± 0.13 vs saline (S) = 2.85 ± 0.59; fMLP: Ptx = 0.43 ± 0.09 and Dex 0.01 ± 0.01 vs S = 1.08 ± 0.15 neutrophil diapedesis/field) and infiltration (LPS: Ptx = 6.29 ± 1.4 and Dex = 3.06 ± 0.76 vs S = 15.94 ± 3.97; fMLP: Ptx = 3.85 ± 0.56 and Dex = 0.40 ± 0.16 vs S = 7.15 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutrophil migration; Pertussis toxin; LPS; Dexamethasone; Mesentery; Histopathological approach.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000013

p16 (INK4a) has clinicopathological and prognostic impact on oropharynx and larynx squamous cell carcinoma
Provedor de dados: 56
Autores: Silva,S.D.; Nonogaki,S.; Soares,F.A.; Kowalski,L.P.
CDKN2A encodes proteins such as p16 (INK4a), which negatively regulate the cell-cycle. Molecular genetic studies have revealed that deletions in CDKN2A occur frequently in cancer. Although p16 (INK4a) may be involved in tumor progression, the clinical impact and prognostic implications in head and neck squamous cell carcinoma (HNSCC) are controversial. The objective of this study was to evaluate the frequency of the immunohistochemical expression of p16 (INK4a) in 40 oropharynx and 35 larynx from HNSCC patients treated in a single institution and followed-up at least for 10 years in order to explore potential associations with clinicopathological outcomes and prognostic implications. Forty cases (53.3%) were positive for p16 (INK4a) and this expression was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Head and neck cancer; Squamous cell carcinoma; Prognostic value; P16.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200032

Smoking and psychiatric disorders: a comorbidity survey
Provedor de dados: 56
Autores: Lopes,F.L.; Nascimento,I.; Zin,W.A.; Valença,A.M.; Mezzasalma,M.A.; Figueira,I.; Nardi,A.E.
Epidemiological and clinical studies have shown a positive correlation between smoking and psychiatric disorders. To investigate the prevalence of cigarette smoking, 277 psychiatric outpatients with anxiety or depressive disorders (DSM-IV) answered a self-evaluation questionnaire about smoking behavior and were compared with a group of 68 control subjects. The diagnoses (N = 262) were: 30.2% (N = 79) major depressive disorder, 23.3% (N = 61) panic disorder, 15.6% (N = 41) social anxiety disorder, 7.3% (N = 19) other anxiety disorders, and 23.7% (N = 62) comorbidity disorders. Among them, 26.3% (N = 69) were smokers, 23.7% (N = 62) were former smokers and 50.0% (N = 131) were nonsmokers. The prevalence of nicotine dependence among the smokers was 59.0%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smoking; Panic disorder; Social anxiety disorder; Depression; Depressive disorder.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800013

Antinociception synergy between the peripheral and spinal sites of the heme oxygenase-carbon monoxide pathway
Provedor de dados: 56
Autores: Nascimento,C.G.O.; Branco,L.G.S.
We have shown that the peripheral and spinal cord heme oxygenase (HO)-carbon monoxide (CO)-soluble guanylate cyclase-cGMP pathways play an important role in antinociception in the rat experimental formalin model. Our objective was to determine if there is synergism between peripheral (paw) and spinal HO-CO pathways in nociception. Rats were handled and adapted to the experimental environment for a few days before the formalin test, in which 50 µL of a 1% formalin was injected subcutaneously into the dorsal surface of the right hind paw. The animals were then observed for 1 h and the frequency of flinching behavior was taken to represent the nociceptive response. Thirty minutes before the test, rats were pretreated with intrathecal injections of the HO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nociception; Carbon monoxide; Formalin; Hypernociception; Antinociception.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100020

Effect of asthma severity on symptom perception in childhood asthma
Provedor de dados: 56
Autores: Cabral,A.L.B.; Conceição,G.M.; Saldiva,P.H.N.; Martins,M.A.
Individual ability to perceive airway obstruction varies substantially. The factors influencing the perception of asthma are probably numerous and not well established in children. The present study was designed to examine the influence of asthma severity, use of preventive medication, age and gender on the association between respiratory symptoms (RS) and peak expiratory flow (PEF) rates in asthmatic children. We followed 92 asthmatic children, aged 6 to 16 years, for five months. Symptom scores were recorded daily and PEF was measured twice a day. The correlations among variables at the within-person level over time were analyzed for each child and for the pooled data by multivariate analysis. After pooling the data, there was a significant (P&lt;0.05)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Asthma severity; Pulmonary function tests; Symptom perception; Childhood asthma.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300006

Biological activity of Serratia marcescens cytotoxin
Provedor de dados: 56
Autores: Carbonell,G.V.; Amorim,C.R.N.; Furumura,M.T.; Darini,A.L.C.; Fonseca,B.A.L.; Yano,T.
Serratia marcescens cytotoxin was purified to homogeneity by ion-exchange chromatography on a DEAE Sepharose Fast Flow column, followed by gel filtration chromatography on a Sephadex G100 column. The molecular mass of the cytotoxin was estimated to be about 50 kDa. Some biological properties of the cytotoxin were analyzed and compared with well-characterized toxins, such as VT1, VT2 and CNF from Escherichia coli and hemolysin produced by S. marcescens. The sensitivity of the cell lines CHO, HeLa, HEp-2, Vero, BHK-21, MA 104 and J774 to the cytotoxin was determined by the cell viability assay using neutral red. CHO and HEp-2 were highly sensitive, with massive cellular death after 1 h of treatment, followed by BHK-21, HeLa, Vero and J774 cells, while MA 104...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell culture; Biological activity; Serratia marcescens; Cytotoxin; Virulence factors.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300010

Expression of merlin, NDRG2, ERBB2, and c-MYC in meningiomas: relationship with tumor grade and recurrence
Provedor de dados: 56
Autores: Ongaratti,B.R.; Silva,C.B.O.; Trott,G.; Haag,T.; Leães,C.G.S.; Ferreira,N.P.; Oliveira,M.C.; Pereira-Lima,J.F.S.
Meningiomas are common, usually benign tumors of the central nervous system that have a high rate of post-surgical recurrence or regrowth. We determined expression of the proteins merlin, NDRG2, ERBB2, and c-MYC in meningiomas using immunohistochemistry and assessed relationships between protein expression and gender, age, tumor grade, and recurrence or regrowth. The study sample comprised 60 patients, (44 women and 16 men) with a mean age of 53.2±12.7 years. Tumors were classified as grade I (n=48) or grades II and III (n=12). Expression of merlin, NDRG2, ERBB2, and c-MYC was not significantly different statistically with relation to gender, age, or meningioma recurrence or regrowth. Merlin was expressed in 100% of the cases. No statistically significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meningioma; Merlin; NDRG2; ERBB2; C-MYC; Immunohistochemistry.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400706

Downregulation of lncRNA CCDC26 contributes to imatinib resistance in human gastrointestinal stromal tumors through IGF-1R upregulation
Provedor de dados: 56
Autores: Yan,Jingyi; Chen,Didi; Chen,Xiaolei; Sun,Xuecheng; Dong,Qiantong; Hu,Changyuan; Zhou,Feng; Chen,Wei.
Imatinib is the first line of therapy for patients with metastatic or gastrointestinal stromal tumors (GIST). However, drug resistance limits the long-term effect of imatinib. Long non-coding RNAs (lncRNAs) are emerging as key players in regulating drug resistance in cancer. In this study, we investigated the association between lncRNA CCDC26 and IGF-1R in GIST and their involvement in drug resistance. Considering the key role of lncRNAs in drug resistance in cancer, we hypothesized that IGF-1R is regulated by lncRNAs. The expression of a series of reported drug resistance-related lncRNAs, including CCDC26, ARF, H19, NBR2, NEAT1, and HOTAIR, in GIST cells treated with imatinib H19 was examined at various time-points by qRT-PCR. Based on our results and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Long non-coding RNA CCDC26; Gastrointestinal stromal tumors; GIST; IGF-1R; Imatinib resistance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600610

Agonistic-like responses from the torus semicircularis dorsalis elicited by GABA A blockade in the weakly electric fish Gymnotus carapo
Provedor de dados: 56
Autores: Duarte,T.T.; Corrêa,S.A.L.; Santana,U.J.; Pereira,A.S.F.; Hoffmann,A.
Findings by our group have shown that the dorsolateral telencephalon of Gymnotus carapo sends efferents to the mesencephalic torus semicircularis dorsalis (TSd) and that presumably this connection is involved in the changes in electric organ discharge (EOD) and in skeletomotor responses observed following microinjections of GABA A antagonist bicuculline into this telencephalic region. Other studies have implicated the TSd or its mammalian homologue, the inferior colliculus, in defensive responses. In the present study, we explore the possible involvement of the TSd and of the GABA-ergic system in the modulation of the electric and skeletomotor displays. For this purpose, different doses of bicuculline (0.98, 0.49, 0.245, and 0.015 mM) and muscimol (15.35...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electric fish; Torus semicircularis dorsalis; GABA-ergic system; Electromotor modulation; Agonistic behavior.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700013

Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU 145 human prostate cancer cells
Provedor de dados: 56
Autores: Vucic,V.; Isenovic,E.R.; Adzic,M.; Ruzdijic,S.; Radojcic,M.B.
Gamma-irradiation (gamma-IR) is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of 60Co gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD), specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma-irradiation; Prostate cancer; Proliferation; Cell death; Cell cycle arrest; Superoxide dismutase.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200009

Protective effects of phosphodiesterase inhibitors on lung function and remodeling in a murine model of chronic asthma
Provedor de dados: 56
Autores: Campos,H.S.; Xisto,D.G.; Oliveira,M.B.G.; Teixeira,I.; Negri,E.M.; Mauad,T.; Carnielli,D.; Lima,L.M.; Barreiro,E.J.; Faffe,D.S.; Zin,W.A.; Lapa e Silva,J.R.; Rocco,P.R.M.
The aim of the present study was to compare the efficacy of a novel phosphodiesterase 4 and 5 inhibitor, LASSBio596, with that of dexamethasone in a murine model of chronic asthma. Lung mechanics (airway resistance, viscoelastic pressure, and static elastance), histology, and airway and lung parenchyma remodeling (quantitative analysis of collagen and elastic fiber) were analyzed. Thirty-three BALB/c mice were randomly assigned to four groups. In the asthma group (N = 9), mice were immunized with 10 µg ovalbumin (OVA, ip) on 7 alternate days, and after day 40 they were challenged with three intratracheal instillations of 20 µg OVA at 3-day intervals. Control mice (N = 8) received saline under the same protocol. In the dexamethasone (N = 8) and LASSBio596...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Asthma; Lung function; Steroid; Phosphodiesterase inhibitors; BALB/c mice.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200016

Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells
Provedor de dados: 56
Autores: Xiao,J.B.; Chen,X.Q.; Zhang,Y.W.; Jiang,X.Y.; Xu,M.
The cytotoxicity of three extracts (petroleum ether, ethyl acetate and n-butanol) from a plant used in folk medicine, Marchantia convoluta, to human non-small cell lung carcinoma (H1299) and liver carcinoma (HepG2) cell lines was tested. After 72-h incubation of lung and liver cancer cell cultures with varying concentrations of extracts (15 to 200 µg/mL), cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and reported in terms of cell viability. The extracts that showed a significant cytotoxicity were subjected to gas chromatography-mass spectrometry analysis to identify the components. The ethyl acetate, but not the petroleum ether or n-butanol extract, had a significant cytotoxicity against lung and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marchantia convoluta extract; Cytotoxicity assay; HepG2 and H1299 carcinoma cell lines; Phytol; Caryophyllene oxide.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600005

Interleukin-10-dependent down-regulation of interferon-gamma response to Leishmania by Mycobacterium leprae antigens during the clinical course of a coinfection
Provedor de dados: 56
Autores: Azeredo-Coutinho,R.B.G.; Matos,D.C.S.; Nery,J.A.C.; Valete-Rosalino,C.M.; Mendonça,S.C.F.
We have described a case of a patient with an intriguing association of mucocutaneous leishmaniasis with lepromatous leprosy, two opposite polar forms of these spectral diseases. In the present follow-up study, we investigated the effect of the addition of Mycobacterium leprae antigens on interferon-gamma (IFN-γ) production in Leishmania antigen-stimulated cultures of peripheral blood mononuclear cells (PBMC) from this patient. For this purpose, PBMC cultures were stimulated with crude L. braziliensis and/or M. leprae whole-cell antigen extracts or with concanavalin A. In some experiments, neutralizing anti-human interleukin (IL)-10 antibodies were added to the cultures. IFN-γ and IL-10 levels in culture supernatants were measured by ELISA. During active...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Interferon-gamma; Interleukin-10; Mucocutaneous leishmaniasis; Lepromatous leprosy; Leishmania braziliensis; Mycobacterium leprae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700010

Effect of practice and span length on the dual-task coordination executive test
Provedor de dados: 56
Autores: Sylwan,R.P.; Rosin,F.M.; Galera,C.
The measure "mu", proposed as an index of the ability to coordinate concurrent box-crossing (BC) and digit-span (DS) tasks in the dual task (DT), should reflect the capacity of the executive component of the working memory system. We investigated the effect of practice in BC and of a change in the digit span on mu by adding previous practice trials in BC and diminishing, maintaining or increasing the digit sequence length. The mu behavior was evaluated throughout three trials of the test. Reported strategies in digit tasks were also analyzed. Subjects with diminished span showed the best performance in DT due to a stable performance in DS and BC in the single- and dual-task conditions. These subjects also showed a more stable performance throughout trials....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dual task; Working memory; Central executive; Digit span; Practice.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000013

The six-minute walk test and body weight-walk distance product in healthy Brazilian subjects
Provedor de dados: 56
Autores: Iwama,A.M.; Andrade,G.N.; Shima,P.; Tanni,S.E.; Godoy,I.; Dourado,V.Z.
We assessed the 6-min walk distance (6MWD) and body weight x distance product (6MWw) in healthy Brazilian subjects and compared measured 6MWD with values predicted in five reference equations developed for other populations. Anthropometry, spirometry, reported physical activity, and two walk tests in a 30-m corridor were evaluated in 134 subjects (73 females, 13-84 years). Mean 6MWD and 6MWw were significantly greater in males than in females (622 ± 80 m, 46,322 ± 10,539 kg.m vs 551 ± 71 m, 36,356 ± 8,289 kg.m, P &lt; 0.05). Four equations significantly overestimated measured 6MWD (range, 32 ± 71 to 137 ± 74 m; P &lt; 0.001), and one significantly underestimated it (-36 ± 86 m; P &lt; 0.001). 6MWD significantly correlated with age (r = -0.39), height (r =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Walking; Exercise; Rehabilitation; 6MWD; 6MWw.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100013

Food intake and weight of lactating rats maintained on different protein-calorie diets, and pup growth
Provedor de dados: 56
Autores: Cambraia,R.P.B.; Vannucchi,H.; De-Oliveira,L.M.
Studies on rats maintained on low-protein-calorie diets during the lactation period show that food intake decreases. This process results in weight loss and a delay in litter development. The purpose of the present study was to determine the alterations in food intake, maternal weight and litter growth during lactation when dams were exposed to diets with different levels of protein and carbohydrate. Female Wistar rats receiving one of 4 different diets, A (N = 14), B (N = 14), C (N = 9) and D (N = 9), were used. Diet A contained 16% protein and 66% carbohydrate; diet B, 6% protein and 77% carbohydrate; diet C, 6% protein and 66% carbohydrate; diet D, 16% protein and 56% carbohydrate. Thus, C and D diets were hypocaloric, while A and B were isocaloric. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactation; Malnutrition; Low-protein-calorie diet; Feeding behavior; Weight and growth of rats.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800012

Monoterpenoids (thymol, carvacrol and S-(+)-linalool) with anesthetic activity in silver catfish (Rhamdia quelen): evaluation of acetylcholinesterase and GABAergic activity
Provedor de dados: 56
Autores: Bianchini,A.E.; Garlet,Q.I.; da Cunha,J.A.; Bandeira Junior,G.; Brusque,I.C.M.; Salbego,J.; Heinzmann,B.M.; Baldisserotto,B.
This study evaluated the anesthetic potential of thymol and carvacrol, and their influence on acetylcholinesterase (AChE) activity in the muscle and brain of silver catfish (Rhamdia quelen). The AChE activity of S-(+)-linalool was also evaluated. We subsequently assessed the effects of thymol and S-(+)-linalool on the GABAergic system. Fish were exposed to thymol and carvacrol (25, 50, 75, and 100 mg/L) to evaluate time for anesthesia and recovery. Both compounds induced sedation at 25 mg/L and anesthesia with 50–100 mg/L. However, fish exposed to carvacrol presented strong muscle contractions and mortality. AChE activity was increased in the brain of fish at 50 mg/L carvacrol and 100 mg/L thymol, and decreased in the muscle at 100 mg/L carvacrol....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AChE; Anesthesia; Benzodiazepine; Fish; GABAA; Terpenoids.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200610

Rhein induces apoptosis of human gastric cancer SGC-7901 cells via an intrinsic mitochondrial pathway
Provedor de dados: 56
Autores: Li,Yiwen; Xu,Yuqing; Lei,Bo; Wang,Wenxiu; Ge,Xin; Li,Jingrui.
Rhein is a primary anthraquinone found in the roots of a traditional Chinese herb, rhubarb, and has been shown to have some anticancer effects. The aim of the present study was to investigate the effect of rhein on the apoptosis of the human gastric cancer line SGC-7901 and to identify the mechanism involved. SGC-7901 cells were cultured and treated with rhein (0, 50, 100, 150, and 200 µM) for 24, 48, or 72 h. Relative cell viability assessed by the MTT assay after treatment was 100, 99, 85, 79, 63% for 24 h; 100, 98, 80, 51, 37% for 48 h, and 100, 97, 60, 36, 15% for 72 h, respectively. Cell apoptosis was detected with TUNEL staining and quantified with flow cytometry using annexin FITC-PI staining at 48 h after 100, 200 and 300 µm rhein. The percentage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhein; SGC-7901 cells; MTT; TUNEL; Flow cytometry; Apoptosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100009

Performance of a Brazilian population sample in the Boston Diagnostic Aphasia Examination: A pilot study
Provedor de dados: 56
Autores: Radanovic,M.; Mansur,L.L.
Brazilian researchers and health professionals often face the challenge of having to use tests developed in foreign languages and standardized for populations of other countries, especially in the fields of Neuropsychology and Neurolinguistics. This fact promotes a feeling that some scoring systems may be inadequate for our sociocultural reality. In the present study, we describe the performance of a Brazilian population sample submitted to a translated and adapted version of the Boston Diagnostic Aphasia Examination (BDAE). Sixty normal volunteers (21 men and 39 women), all Portuguese native speakers, ranging in age from 15 to 78 years (average 43.7) and with an educational level of 2 to 16 years (average 9.9), were tested using a translated and adapted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aphasia; Diagnosis; Portuguese.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300005

Ethanol-induced hypothermia in rats is antagonized by dexamethasone
Provedor de dados: 56
The effect of dexamethasone on ethanol-induced hypothermia was investigated in 3.5-month old male Wistar rats (N = 10 animals per group). The animals were pretreated with dexamethasone (2.0 mg/kg, ip; volume of injection = 1 ml/kg) 15 min before ethanol administration (2.0, 3.0 and 4.0 g/kg, ip; 20% w/v) and the colon temperature was monitored with a digital thermometer 30, 60 and 90 min after ethanol administration. Ethanol treatment produced dose-dependent hypothermia throughout the experiment (-1.84 ± 0.10, -2.79 ± 0.09 and -3.79 ± 0.15oC for 2.0, 3.0 and 4.0 g/kg ethanol, respectively, 30 min after ethanol) but only the effects of 2.0 and 3.0 g/kg ethanol were significantly antagonized (-0.57 ± 0.09 and -1.25 ± 0.10, respectively, 30 min after ethanol)...
Palavras-chave: Ethanol; Dexamethasone; Hypothermia; Glucocorticoids; Rats.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100011

No evidence of association of MUC-1 genetic polymorphism with embryo implantation failure
Provedor de dados: 56
Autores: Dentillo,D.B.; Souza,F.R.P.; Meola,J.; Vieira,G.S.; Yazlle,M.E.H.D.; Goulart,L.R.; Martelli,L.
Pregnancy loss can be caused by several factors involved in human reproduction. Although up to 50% of cases remain unexplained, it has been postulated that the major cause of failed pregnancy is an error of embryo implantation. Transmembrane mucin-1 (MUC-1) is a glycoprotein expressed on the endometrial cell surface which acts as a barrier to implantation. The gene that codes for this molecule is composed of a polymorphic tandem repeat of 60 nucleotides. Our objective was to determine if MUC-1 genetic polymorphism is associated with implantation failure in patients with a history of recurrent abortion. The study was conducted on 10 women aged 25 to 35 years with no history of successful pregnancy and with a diagnosis of infertility. The control group...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mucin-1; Female infertility; Implantation failure; Variable number of tandem repeats; Genetic polymorphism.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600007

CTLA4 enhances the osteogenic differentiation of allogeneic human mesenchymal stem cells in a model of immune activation
Provedor de dados: 56
Autores: Dai,F.; Zhang,F.; Sun,D.; Zhang,Z.H.; Dong,S.W.; Xu,J.Z.
Allogeneic mesenchymal stem cells (allo-MSCs) have recently garnered increasing interest for their broad clinical therapy applications. Despite this, many studies have shown that allo-MSCs are associated with a high rate of graft rejection unless immunosuppressive therapy is administered to control allo-immune responses. Cytotoxic T-lymphocyte-associated protein 4 (CTLA4) is a co-inhibitory molecule expressed on T cells that mediates the inhibition of T-cell function. Here, we investigated the osteogenic differentiation potency of allo-MSCs in an activated immune system that mimics the in vivo allo-MSC grafting microenvironment and explored the immunomodulatory role of the helper T cell receptor CTLA4 in this process. We found that MSC osteogenic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CTLA4; Mesenchymal stem cells; Immunomodulatory; Bone tissue engineering.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700629

Education, tobacco smoking, alcohol consumption, and IL-2 and IL-6 gene polymorphisms in the survival of head and neck cancer
Provedor de dados: 56
Autores: López,R.V.M.; Zago,M.A.; Eluf-Neto,J.; Curado,M.P.; Daudt,A.W.; da Silva-Junior,W.A.; Zanette,D.L.; Levi,J.E.; de Carvalho,M.B.; Kowalski,L.P.; Abrahão,M.; de Góis-Filho,J.F.; Boffetta,P.; Wünsch-Filho,V.
The association of education, tobacco smoking, alcohol consumption, and interleukin-2 (IL-2 +114 and -384) and -6 (IL-6 -174) DNA polymorphisms with head and neck squamous cell carcinoma (HNSCC) was investigated in a cohort study of 445 subjects. IL-2 and IL-6 genotypes were determined by real-time PCR. Cox regression was used to estimate hazard ratios (HR) and 95% confidence intervals (95%CI) of disease-specific survival according to anatomical sites of the head and neck. Mean age was 56 years and most patients were males (87.6%). Subjects with 5 or more years of schooling had better survival in larynx cancer. Smoking had no effect on HNSCC survival, but alcohol consumption had a statistically significant effect on larynx cancer. IL-2 gene +114 G/T (HR =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smoking; Alcohol; Interleukin; Head and neck cancer; Cancer prognosis; Survival analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000005

Mesenteric lymph reperfusion exacerbates spleen injury caused by superior mesenteric artery occlusion shock
Provedor de dados: 56
Autores: Li,L.L.; Zhang,C.H.; Liu,J.C.; Yang,L.N.; Niu,C.Y.; Zhao,Z.G.
The intestinal lymph pathway plays an important role in the pathogenesis of organ injury following superior mesenteric artery occlusion (SMAO) shock. We hypothesized that mesenteric lymph reperfusion (MLR) is a major cause of spleen injury after SMAO shock. To test this hypothesis, SMAO shock was induced in Wistar rats by clamping the superior mesenteric artery (SMA) for 1 h, followed by reperfusion for 2 h. Similarly, MLR was performed by clamping the mesenteric lymph duct (MLD) for 1 h, followed by reperfusion for 2 h. In the MLR+SMAO group rats, both the SMA and MLD were clamped and then released for reperfusion for 2 h. SMAO shock alone elicited: 1) splenic structure injury, 2) increased levels of malondialdehyde, nitric oxide (NO), intercellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenteric lymph reperfusion; Superior mesenteric artery; Shock; Spleen injury; Endotoxin; Inflammation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500376

Influence of exercise modality on agreement between gas exchange and heart rate variability thresholds
Provedor de dados: 56
Autores: Cunha,F.A.; Montenegro,R.A.; Midgley,A.W.; Vasconcellos,F.; Soares,P.P.; Farinatti,P.
The main purpose of this study was to investigate the level of agreement between the gas exchange threshold (GET) and heart rate variability threshold (HRVT) during maximal cardiopulmonary exercise testing (CPET) using three different exercise modalities. A further aim was to establish whether there was a 1:1 relationship between the percentage heart rate reserve (%HRR) and percentage oxygen uptake reserve (   %    V ˙ O 2 R) at intensities corresponding to GET and HRVT. Sixteen apparently healthy men 17 to 28 years of age performed three maximal CPETs (cycling, walking, and running). Mean heart rate and       V ˙ O 2 at GET and HRVT were 16 bpm (P&lt;0.001) and 5.2 mL·kg-1·min-1 (P=0.001) higher in running than cycling, but no significant differences were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aerobic exercise; Autonomic nervous system; Heart rate reserve; Oxygen uptake reserve; Poincare plot; Ramp protocol.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800706

BAFF promotes regulatory T-cell apoptosis and blocks cytokine production by activating B cells in primary biliary cirrhosis
Provedor de dados: 56
Autores: Zhang,Bo; Hu,Mintao; Zhang,Peng; Cao,Hong; Wang,Yongzhen; Wang,Zheng; Su,Tingting.
Primary biliary cirrhosis (PBC) is a chronic and slowly progressive cholestatic liver disease of autoimmune etiology. A number of questions regarding its etiology are unclear. CD4+CD25+ regulatory T cells (Tregs) play a critical role in self-tolerance and, for unknown reasons, their relative number is reduced in PBC patients. B-cell-activating factor (BAFF) is a key survival factor during B-cell maturation and its concentration is increased in peripheral blood of PBC patients. It has been reported that activated B cells inhibit Treg cell proliferation and there are no BAFF receptors on Tregs. Therefore, we speculated that excessive BAFF may result in Treg reduction via B cells. To prove our hypothesis, we isolated Tregs and B cells from PBC and healthy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Primary biliary cirrhosis; Regulatory T cells; BAFF; B cells; Bezafibrate.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500433

Does the correlation between EBNA-1 and p63 expression in breast carcinomas provide a clue to tumorigenesis in Epstein-Barr virus-related breast malignancies?
Provedor de dados: 56
Autores: Ribeiro-Silva,A.; Ramalho,L.N.Z.; Garcia,S.B.; Zucoloto,S.
Several investigators have identified Epstein-Barr virus (EBV) particles in breast carcinomas, a fact that supports a role for EBV in mammary tumorigenesis. The possible mechanism involved in this process is not clear. The present study was carried out in an attempt to determine whether there is a relationship between latent infection with EBV and p53 and p63 expression in breast carcinomas. Immunohistochemistry developed with 3.3-diaminobenzidine tetrahydrochloride was performed in 85 formalin-fixed paraffin-embedded breast carcinomas using anti-EBV EBNA-1, anti-p63, anti-p53, anti-estrogen receptor (ER) and anti-progesterone receptor (PR) antibodies. The cases were selected to represent each of the various histologic types: intraductal carcinoma (N =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EBNA-1; P63; P53; Breast; Carcinoma; Tumorigenesis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100013

Effect of aging and oral tolerance on dendritic cell function
Provedor de dados: 56
Autores: Simioni,P.U.; Fernandes,L.G.R.; Gabriel,D.L.; Tamashiro,W.M.S.C.
Oral tolerance can be induced in some mouse strains by gavage or spontaneous ingestion of dietary antigens. In the present study, we determined the influence of aging and oral tolerance on the secretion of co-stimulatory molecules by dendritic cells (DC), and on the ability of DC to induce proliferation and cytokine secretion by naive T cells from BALB/c and OVA transgenic (DO11.10) mice. We observed that oral tolerance could be induced in BALB/c mice (N = 5 in each group) of all ages (8, 20, 40, 60, and 80 weeks old), although a decline in specific antibody levels was observed in the sera of both tolerized and immunized mice with advancing age (40 to 80 weeks old). DC obtained from young, adult and middle-aged (8, 20, and 40 weeks old) tolerized mice were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Oral tolerance; Dendritic cell; Antibody; Proliferation; Cytokine secretion.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100010

Predictors of restenosis after percutaneous coronary intervention using bare-metal stents: a comparison between patients with and without dysglycemia
Provedor de dados: 56
Autores: Lima-Filho,M.O.; Figueiredo,G.L.; Foss-Freitas,M.C.; Foss,M.C.; Marin-Neto,J.A.
The objective of this study was to identify intravascular ultrasound (IVUS), angiographic and metabolic parameters related to restenosis in patients with dysglycemia. Seventy consecutive patients (77 lesions) selected according to inclusion and exclusion criteria were evaluated by the oral glucose tolerance test and the determination of insulinemia after a successful percutaneous coronary intervention (PCI) with a bare-metal stent. The degree of insulin resistance was calculated by the homeostasis model assessment of insulin resistance (HOMA-IR). Six-month IVUS and angiogram follow-up were performed. Thirty-nine patients (55.7%) had dysglycemia. The restenosis rate in the dysglycemic group was 37.2 vs 23.5% in the euglycemic group (P = 0.299). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary stents; Coronary restenosis; Insulin resistance; Diabetes mellitus; Dysglycemia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600008

Food and the circadian activity of the hypothalamic-pituitary-adrenal axis
Provedor de dados: 56
Autores: Leal,A.M.O.; Moreira,A.C.
Temporal organization is an important feature of biological systems and its main function is to facilitate adaptation of the organism to the environment. The daily variation of biological variables arises from an internal time-keeping system. The major action of the environment is to synchronize the internal clock to a period of exactly 24 h. The light-dark cycle, food ingestion, barometric pressure, acoustic stimuli, scents and social cues have been mentioned as synchronizers or" zeitgebers". The circadian rhythmicity of plasma corticosteroids has been well characterized in man and in rats and evidence has been accumulated showing daily rhythmicity at every level of the hypothalamic-pituitary-adrenal (HPA) axis. Studies of restricted feeding in rats are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feeding; Glucocorticoids; CRH; ACTH; Circadian rhythm.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200003

A calcineurin inhibitory protein overexpressed in Down's syndrome interacts with the product of a ubiquitously expressed transcript
Provedor de dados: 56
Autores: Silveira,H.C.S.; Sommer,C.A.; Soares-Costa,A.; Henrique-Silva,F.
The Down's syndrome candidate region 1 (DSCR1) protein, encoded by a gene located in the human chromosome 21, interacts with calcineurin and is overexpressed in Down's syndrome patients. As an approach to clarifying a putative function for this protein, in the present study we used the yeast two-hybrid system to identify DSCR1 partners. The two-hybrid system is a method that allows the identification of protein-protein interactions through reconstitution of the activity of the yeast GAL 4 transcriptional activator. The gene DSCR1 fused to the GAL 4 binding domain (BD) was used to screen a human fetal brain cDNA library cloned in fusion with the GAL 4 activation domain (AD). Three positive clones were found and sequence analysis revealed that all the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Down's syndrome; Down's syndrome candidate region 1; Calcineurin; Two-hybrid system; Ubiquitously expressed transcript; Protein interactions.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600002

Blockade of the action of nitric oxide in human septic shock increases systemic vascular resistance and has detrimental effects on pulmonary function after a short infusion of methylene blue
Provedor de dados: 56
Autores: Weingartner,R.; Oliveira,E.; Oliveira,E.S.; Sant'Anna,U.L.; Oliveira,R.P.; Azambuja,L.A.; Friedman,G.
To investigate the role of nitric oxide in human sepsis, ten patients with severe septic shock requiring vasoactive drug therapy and mechanical ventilation were enrolled in a prospective, open, non-randomized clinical trial to study the acute effects of methylene blue, an inhibitor of guanylate cyclase. Hemodynamic and metabolic variables were measured before and 20, 40, 60, and 120 min after the start of a 1-h intravenous infusion of 4 mg/kg of methylene blue. Methylene blue administration caused a progressive increase in mean arterial pressure (60 [55-70] to 70 [65-100] mmHg, median [25-75th percentiles]; P&amp;lt;0.05), systemic vascular resistance index (649 [479-1084] to 1066 [585-1356] dyne s-1 cm-5 m-2; P&amp;lt;0.05) and the left ventricular stroke...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vasodilation; Myocardial depression; Septic shock; Methylene blue; Nitric oxide; Guanylate cyclase.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200009

Sex-dependent differences in the activities of acetylsalicylic acid-esterases in mouse kidneys
Provedor de dados: 56
Autores: Benedito,M.A.C.
Acetylsalicylic acid (ASA), the most used drug worldwide, is hydrolyzed to salicylic acid and acetate by esterases present in tissues of several species including humans. Sex differences in drug metabolism by rodent liver are documented in the literature. In this paper we report a difference in the activities of the esterases (ASA-esterase I and II) in the kidneys of male and female mice. In this species there is no difference between males and females in liver ASA-esterases (ASA-esterase I: males 38.5 ± 7.9 (N = 5) and females 31.6 ± 7.6 (N = 5) nmol of salicylic acid formed min-1 mg protein-1, P&amp;gt;0.05; ASA-esterase II: males 77.3 ± 17.4 (N = 5) and females 61.4 ± 15.1 (N = 5) nmol of salicylic acid formed min-1 mg protein-1, P&amp;gt;0.05)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylsalicylic acid; Esterases; Mice; Sex differences; Kidneys; Liver.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300004

The role of micro-ribonucleic acids in normal hematopoiesis and leukemic T-lymphogenesis
Provedor de dados: 56
Autores: Slavov,S.N.; Gimenes Teixeira,H.L.; Rego,E.M.
Micro-ribonucleic acids (microRNAs) are small molecules containing 20-23 nucleotides. Despite their small size, it is likely that almost every cellular process is regulated by them. Moreover, aberrant microRNA expression has been involved in the development of various diseases, including cancer. Although many data are available about the role of microRNAs in various lymphoproliferative disorders, their impact on the development of acute lymphoblastic leukemia of T-cell progenitors is largely unknown. In this review, we present recent information about how specific microRNAs are expressed and regulated during malignant T-lymphopoiesis and about their role during normal hematopoiesis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNAs; Gene expression; Leukemia; Acute lymphoblastic leukemia; T cells.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700003

A 28-fold increase in secretory protein synthesis is associated with DNA puff activity in the salivary gland of Bradysia hygida (Diptera, Sciaridae)
Provedor de dados: 56
When the first group of DNA puffs is active in the salivary gland regions S1 and S3 of Bradysia hygida larvae, there is a large increase in the production and secretion of new salivary proteins demonstrable by [3H]-Leu incorporation. The present study shows that protein separation by SDS-PAGE and detection by fluorography demonstrated that these polypeptides range in molecular mass from about 23 to 100 kDa. Furthermore, these proteins were synthesized mainly in the S1 and S3 salivary gland regions where the DNA puffs C7, C5, C4 and B10 are conspicuous, while in the S2 region protein synthesis was very low. Others have shown that the extent of amplification for DNA sequences that code for mRNA in the DNA puffs C4 and B10 was about 22 and 10 times,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gene amplification; Secretory polypeptide synthesis; DNA puffs; Salivary glands of Sciaridae.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500006

Do early life factors influence body mass index in adolescents?
Provedor de dados: 56
Autores: Goldani,M.Z.; Haeffner,L.S.B.; Agranonik,M.; Barbieri,M.A.; Bettiol,H.; Silva,A.A.M.
The association between early life factors and body mass index (BMI) in adulthood has been demonstrated in developed countries. The aim of the present study was to assess the influence of early life factors (birth weight, gestational age, maternal smoking, and social class) on BMI in young adulthood with adjustment for adult socioeconomic position. A cohort study was carried out in 1978/79 with 6827 mother-child pairs from Ribeirão Preto city, located in the most developed economic area of the country. Biological, economic and social variables and newborn anthropometric measurements were obtained shortly after delivery. In 1996, 1189 males from this cohort, 34.3% of the original male population, were submitted to anthropometric measurements and were asked...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adolescence; Body mass index; Perinatal factors; Life stile.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900010

An optically coupled power stimulus isolation unit with high voltage and fast rise time output
Provedor de dados: 56
Autores: Brasil,R.O.; Leal-Cardoso,J.H.
Recent technological developments have created new devices that could improve and simplify the construction of stimulus isolators. HEXFET transistors can switch large currents and hundreds of volts in nanoseconds. The newer opto-isolators can give a pulse rise time of a few nanoseconds, with output compatible with MOSFET devices, in which delays are reduced to nanoseconds. Integrated DC/DC converters are now available. Using these new resources we developed a new electrical stimulus isolator circuit with selectable constant-current and constant-voltage modes, which are precise and easy to construct. The circuit works like a regulated power supply in both modes with output switched to zero or to free mode through an opto-isolator device. The isolator...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Electrical isolation; Electrical stimulation; Pulse stimulation; Stimulus isolator.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600013

Effect of inhibitory avoidance training on [3H]-glutamate binding in the hippocampus and parietal cortex of rats
Provedor de dados: 56
Autores: Schröder,N.; de-Paris,F.; Roesler,R.; Medina,J.H.; Souza,D.O.; Izquierdo,I.
Glutamate receptors have been implicated in memory formation. The aim of the present study was to determine the effect of inhibitory avoidance training on specific [3H]-glutamate binding to membranes obtained from the hippocampus or parietal cortex of rats. Adult male Wistar rats were trained (0.5-mA footshock) in a step-down inhibitory avoidance task and were sacrificed 0, 5, 15 or 60 min after training. Hippocampus and parietal cortex were dissected and membranes were prepared and incubated with 350 nM [3H]-glutamate (N = 4-6 per group). Inhibitory avoidance training induced a 29% increase in glutamate binding in hippocampal membranes obtained from rats sacrificed at 5 min (P&amp;lt;0.01), but not at 0, 15, or 60 min after training, and did not affect...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glutamate receptor hippocampus parietal cortex long-term potentiation emotional learning; Memory.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200011

Impact of cigarette smoke and aerobic physical training on histological and molecular markers of prostate health in rats
Provedor de dados: 56
Autores: Veras,A.S.C.; Baptista,D.B.; dos Santos,N.J.; Thorpe,H.H.A.; Seraphim,P.M.; Florido Neto,A.R.; Teixeira,G.R.
Recent evidence suggests that aerobic physical training may attenuate the deleterious effects of cancer risk factors, including smoking. We investigated the effects of cigarette smoke inhalation and aerobic physical training on the expression of steroid receptors and inflammatory and apoptotic proteins in the prostate. Forty male Wistar rats were distributed in four groups: control (CO), exercise (EXE), cigarette smoke exposure (CS), and cigarette smoke exposure with exercise (CS+EXE). For eight weeks, animals were repeatedly exposed to cigarette smoke for 30 min or performed aerobic physical training either with or without the cigarette smoke inhalation protocol. Following these experiments, we analyzed prostate epithelial morphology and prostatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Androgen receptor; Growth factors; Endurance exercise; BAX; BCL-2.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500601

Low frequency of p53 mutations in cervical carcinomas among Brazilian women
Provedor de dados: 56
Autores: Pinheiro,N.A.; Villa,L.L.
Human papillomavirus (HPV) infections of the high-risk types are strongly linked to the development of cervical carcinoma. The HPV oncoproteins E6 and E7 are thought to play a crucial role in this process through their interactions with the p53 protein and the retinoblastoma susceptibility gene product pRb, respectively. E6 binds to p53 protein promoting its degradation. This is considered to contribute to the oncogenesis of HPV-associated anogenital cancer. On the other hand, in HPV-negative cervical carcinoma, p53 mutations are thought to have a role in the transformation process. A total of 122 HPV-positive cervical carcinoma tissue samples were evaluated for the presence of mutations in exons 5-8 of the p53 gene by single-stranded conformation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single-stranded conformation polymorphism; SSCP; Suppressor gene; HPV; PCR; DNA sequencing.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600005

MALAT1 is involved in type I IFNs-mediated systemic lupus erythematosus by up-regulating OAS2, OAS3, and OASL
Provedor de dados: 56
Autores: Gao,Fei; Tan,Yuan; Luo,Hong.
Systemic lupus erythematosus (SLE) is an autoimmune disease associated with an aberrant activation of immune cells partly due to the dysfunction of cytokines such as type I interferons (IFNs). Long non-coding RNA MALAT1 has been found to play a pathogenic role in SLE; however, the underlying mechanisms are still poorly understood. Bioinformatics analysis showed the up-regulation of type I IFN downstream effectors OAS2, OAS3, and OASL (OAS-like) in CD4+ T cells, CD19+ B cells, and CD33+ myeloid cells in patients with active SLE compared to healthy participants. In this study, peripheral blood mononuclear cells (PBMCs), CD19+ B, and CD4+ T cells were isolated from active SLE patients and healthy participants. PCR was performed to quantify MALAT1, OAS2, OAS3,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MALAT1; OAS2; OAS3; OASL; Systemic lupus erythematosus; Type I IFNs.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500603

Distribution of biglycan and decorin in rat dental tissue
Provedor de dados: 56
Autores: Tenório,D.M.H.; Santos,M.F.; Zorn,T.M.T.
Biglycan and decorin are small leucine-rich proteoglycans that play several biological and structural roles in different tissues and organs. Several reports have indicated that biglycan participates in odontoblast and ameloblast differentiation and in the calcification process. In the present study we show that the expression of biglycan changes from within the ameloblasts and odontoblasts to the extracellular space according to the stage of animal development. In predentin and in the pulp space, however, biglycan was continually expressed throughout the period of investigation. In contrast, decorin was absent in odontoblasts and in ameloblasts and was exclusively expressed in predentin throughout the period of observation. In young rats, however, decorin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Decorin; Biglycan; Odontoblasts; Predentin; Immunohistochemistry.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800012

Comparison of the neurobiological effects of attribution retraining group therapy with those of selective serotonin reuptake inhibitors
Provedor de dados: 56
Autores: Wang,C.; Zhang,N.; Zhang,Y.L.; Zhang,J.; Yang,H.; Timothy,T.C.
The aim of this study was to compare the effectiveness of attribution retraining group therapy (ARGT) with selective serotonin reuptake inhibitors (SSRIs) in the treatment of major depressive disorder (MDD), generalized anxiety disorder (GAD), and obsessive-compulsive disorder (OCD). Subjects were sequentially recruited and randomized into two groups, one receiving ARGT (n = 63) and the other SSRIs (n = 66) for 8 weeks. Fifty-four ARGT outpatients with MDD (n = 19), GAD (n = 19), and OCD (n = 16) and 55 SSRI outpatients with MDD (n = 19), GAD (n = 19), and OCD (n = 17) completed the study. All subjects were assessed using the Hamilton Depression Scale and Hamilton Anxiety Scale before and after treatment. The 10-item Yale-Brown Obsessive Compulsive Scale...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attribution retraining group therapy; Selective serotonin reuptake inhibitors; Neurobiological effects; Serotonin; Cortisol.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300318

Respiratory panic disorder subtype and sensitivity to the carbon dioxide challenge test
Provedor de dados: 56
Autores: Valença,A.M.; Nardi,A.E.; Nascimento,I.; Zin,W.A.; Versiani,M.
The aim of the present study was to verify the sensitivity to the carbon dioxide (CO2) challenge test of panic disorder (PD) patients with respiratory and nonrespiratory subtypes of the disorder. Our hypothesis is that the respiratory subtype is more sensitive to 35% CO2. Twenty-seven PD subjects with or without agoraphobia were classified into respiratory and nonrespiratory subtypes on the basis of the presence of respiratory symptoms during their panic attacks. The tests were carried out in a double-blind manner using two mixtures: 1) 35% CO2 and 65% O2, and 2) 100% atmospheric compressed air, 20 min apart. The tests were repeated after 2 weeks during which the participants in the study did not receive any psychotropic drugs. At least 15 of 16 (93.7%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CO2; Anxiety; Breathing; Respiration.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700004

Paraquat poisoning: an experimental model of dose-dependent acute lung injury due to surfactant dysfunction
Provedor de dados: 56
Autores: Silva,M.F.R.; Saldiva,P.H.N.
Since the most characteristic feature of paraquat poisoning is lung damage, a prospective controlled study was performed on excised rat lungs in order to estimate the intensity of lesion after different doses. Twenty-five male, 2-3-month-old non-SPF Wistar rats, divided into 5 groups, received paraquat dichloride in a single intraperitoneal injection (0, 1, 5, 25, or 50 mg/kg body weight) 24 h before the experiment. Static pressure-volume (PV) curves were performed in air- and saline-filled lungs; an estimator of surface tension and tissue works was computed by integrating the area of both curves and reported as work/ml of volume displacement. Paraquat induced a dose-dependent increase of inspiratory surface tension work that reached a significant two-fold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paraquat; Experimental model; Acute lung injury; Pressure-volume curve; Respiratory work.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300018

Targeting PPM1D by lentivirus-mediated RNA interference inhibits the tumorigenicity of bladder cancer cells
Provedor de dados: 56
Autores: Wang,W.; Zhu,H.; Zhang,H.; Zhang,L.; Ding,Q.; Jiang,H.
Protein phosphatase magnesium/manganese-dependent 1D (PPM1D) is a p53-induced phosphatase that functions as a negative regulator of stress response pathways and has oncogenic properties. However, the functional role ofPPM1D in bladder cancer (BC) remains largely unknown. In the present study, lentivirus vectors carrying small hairpin RNA (shRNA) targeting PPM1D were used to explore the effects ofPPM1D knockdown on BC cell proliferation and tumorigenesis. shRNA-mediated knockdown of PPM1D significantly inhibited cell growth and colony forming ability in the BC cell lines 5637 and T24. Flow cytometric analysis showed that PPM1D silencing increased the proportion of cells in the G0/G1 phase. Downregulation of PPM1Dalso inhibited 5637 cell tumorigenicity in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein phosphatase magnesium/manganese-dependent 1D; Bladder cancer; Gene silencing; RNA interference; Proliferation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201044

Epilepsy-induced electrocardiographic alterations following cardiac ischemia and reperfusion in rats
Provedor de dados: 56
Autores: Tavares,J.G.P.; Vasques,E.R.; Arida,R.M.; Cavalheiro,E.A.; Cabral,F.R.; Torres,L.B.; Menezes-Rodrigues,F.S.; Jurkiewicz,A.; Caricati-Neto,A.; Godoy,C.M.G.; Gomes da Silva,S.
The present study evaluated electrocardiographic alterations in rats with epilepsy submitted to an acute myocardial infarction (AMI) model induced by cardiac ischemia and reperfusion. Rats were randomly divided into two groups: control (n=12) and epilepsy (n=14). It was found that rats with epilepsy presented a significant reduction in atrioventricular block incidence following the ischemia and reperfusion procedure. In addition, significant alterations were observed in electrocardiogram intervals during the stabilization, ischemia, and reperfusion periods of rats with epilepsy compared to control rats. It was noted that rats with epilepsy presented a significant increase in the QRS interval during the stabilization period in relation to control rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epilepsy; Heart; Acute myocardial infarction; Ischemia; Reperfusion.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200140

Gross proteinuria is a strong risk predictor for cardiovascular mortality in Brazilian type 2 diabetic patients
Provedor de dados: 56
Autores: Cardoso,C.R.L.; Salles,G.F.
Increased proteinuria is recognized as a risk predictor for all-cause and cardiovascular mortality in diabetic patients; however, no study has evaluated these relationships in Brazilian patients. The aim of this study was to investigate the prognostic value of gross proteinuria for all-cause and cardiovascular mortalities and for cardiovascular morbidity in a cohort study of 471 type 2 diabetic individuals followed for up to 7 years. Several clinical, laboratory and electrocardiographic variables were obtained at baseline. The relative risks for all-cause, cardiovascular and cardiac mortalities and for cardiovascular and cardiac events associated with the presence of overt proteinuria (&gt;0.5 g/24 h) were assessed by Kaplan-Meier survival curves and by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: All-cause mortality; Cardiovascular mortality; Cardiovascular morbidity; Proteinuria; Type 2 diabetes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800006

The novel p.E89K mutation in the SRY gene inhibits DNA binding and causes the 46,XY disorder of sex development
Provedor de dados: 56
Autores: Cunha,J.L.; Soardi,F.C.; Bernardi,R.D.; Oliveira,L.E.C.; Benedetti,C.E.; Guerra-Junior,G.; Maciel-Guerra,A.T.; de Mello,M.P.
Male sex determination in humans is controlled by the SRY gene, which encodes a transcriptional regulator containing a conserved high mobility group box domain (HMG-box) required for DNA binding. Mutations in the SRY HMG-box affect protein function, causing sex reversal phenotypes. In the present study, we describe a 19-year-old female presenting 46,XY karyotype with hypogonadism and primary amenorrhea that led to the diagnosis of 46,XY complete gonadal dysgenesis. The novel p.E89K missense mutation in the SRY HMG-box was identified as a de novo mutation. Electrophoretic mobility shift assays showed that p.E89K almost completely abolished SRY DNA-binding activity, suggesting that it is the cause of SRY function impairment. In addition, we report the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gonadal dysgenesis; HMG-box; Missense mutation; Sex reversal SRY; Streak gonads; Testis determination.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400014

Cytoadhesion of Plasmodium falciparum-infected erythrocytes and the infected placenta: a two-way pathway
Provedor de dados: 56
Autores: Costa,F.T.M.; Avril,M.; Nogueira,P.A.; Gysin,J.
Malaria is undoubtedly the world's most devastating parasitic disease, affecting 300 to 500 million people every year. Some cases of Plasmodium falciparum infection progress to the deadly forms of the disease responsible for 1 to 3 million deaths annually. P. falciparum-infected erythrocytes adhere to host receptors in the deep microvasculature of several organs. The cytoadhesion of infected erythrocytes to placental syncytiotrophoblast receptors leads to pregnancy-associated malaria (PAM). This specific maternal-fetal syndrome causes maternal anemia, low birth weight and the death of 62,000 to 363,000 infants per year in sub-Saharan Africa, and thus has a poor outcome for both mother and fetus. However, PAM and non-PAM parasites have been shown to differ...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plasmodium falciparum; Cytoadhesion; Pregnancy-associated malaria; Var2CSA gene.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200003

Acute but not chronic metabolic acidosis potentiates the acetylcholine-induced reduction in blood pressure: an endothelium-dependent effect
Provedor de dados: 56
Autores: Celotto,A.C.; Ferreira,L.G.; Capellini,V.K.; Albuquerque,A.A.S.; Rodrigues,A.J.; Evora,P.R.B.
Metabolic acidosis has profound effects on vascular tone. This study investigated the in vivo effects of acute metabolic acidosis (AMA) and chronic metabolic acidosis (CMA) on hemodynamic parameters and endothelial function. CMA was induced by ad libitum intake of 1% NH4Cl for 7 days, and AMA was induced by a 3-h infusion of 6 M NH4Cl (1 mL/kg, diluted 1:10). Phenylephrine (Phe) and acetylcholine (Ach) dose-response curves were performed by venous infusion with simultaneous venous and arterial blood pressure monitoring. Plasma nitrite/nitrate (NOx) was measured by chemiluminescence. The CMA group had a blood pH of 7.15±0.03, which was associated with reduced bicarbonate (13.8±0.98 mmol/L) and no change in the partial pressure of arterial carbon dioxide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acid-base balance; Metabolic acidosis; Hemodynamic failure; Endothelium; Nitric oxide.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200604

Identification of a novel UMOD mutation (c.163G&amp;gt;A) in a Brazilian family with autosomal dominant tubulointerstitial kidney disease
Provedor de dados: 56
Autores: Lopes,L.B.; Abreu,C.C.; Souza,C.F.; Guimaraes,L.E.R.; Silva,A.A.; Aguiar-Alves,F.; Kidd,K.O.; Kmoch,S.; Bleyer,A.J.; Almeida,J.R.
Autosomal dominant tubulointerstitial kidney disease (ADTKD) is characterized by autosomal dominant inheritance, progressive chronic kidney disease, and a bland urinary sediment. ADTKD is most commonly caused by mutations in the UMOD gene encoding uromodulin (ADTKD-UMOD). We herein report the first confirmed case of a multi-generational Brazilian family with ADTKD-UMOD, caused by a novel heterozygous mutation (c.163G&gt;A, GGC→AGC, p.Gly55Ser) in the UMOD gene. Of 41 family members, 22 underwent genetic analysis, with 11 individuals found to have this mutation. Three affected individuals underwent hemodialysis, one peritoneal dialysis, and one patient received a kidney transplant from a family member later found to be genetically affected. Several younger...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic kidney disease; Uromodulin kidney disease; Genetic mutation; Uromodulin; UMOD; Genetic kidney diseases.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300616

Lymphocyte subpopulations during cytomegalovirus disease in renal transplant recipients
Provedor de dados: 56
Autores: Castro,S.M.; Sporleder,H.; Schröeder,R.; Santos,A.; Garcia,V.; Neumann,J.; Costa,S.C.B.
We have determined the number of circulating T, B and natural killer cells in renal transplant recipients in order to detect changes during cytomegalovirus (CMV) infections. Serial blood samples were taken from 61 patients on standard triple immunosuppression therapy (cyclosporin A, azathioprine and prednisone). Using two-color flow cytometry analysis, the absolute number of CD3+, CD4+, CD8+, CD19+, CD3+HLA-DR+ and CD16+56+ cells was determined. Forty-eight patients (78.7%) developed active CMV infection, and all of them subsequently recovered. Twenty of the infected patients (32.8%) presented symptoms compatible with CMV disease during the infectious process. The number of lymphocytes and their main subpopulations were normal before the onset of CMV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transplantation; Cytomegalovirus; Antigenemia; T lymphocytes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600016

Rimonabant improves metabolic parameters partially attributed to restoration of high voltage-activated Ca2+ channels in skeletal muscle in HFD-fed mice
Provedor de dados: 56
Autores: Chen,B.; Hu,N.
Cannabinoid type 1 receptor (CB1R) inhibition tends to be one of the promising strategies for the treatment of obesity and other related metabolic disorders. Although CB1R inhibition may cause adverse psychiatric effects including depression and anxiety, the investigation of the role of peripheral CB1R on weight loss and related metabolic parameters are urgently needed. We first explored the effect of rimonabant, a selective CB1R antagonist/inverse agonist, on some metabolic parameters in high fat-diet (HFD)-induced obesity in mice. Then, real-time PCR and electrophysiology were used to explore the contribution of high voltage-activated Ca2+ channels (HVACCs), especially Cav1.1, on rimonabant's effect in skeletal muscle (SM) in HFD-induced obesity....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cannabinoid receptor type 1; Obesity; Rimonabant; Skeletal Muscle; HVA calcium channel.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600602

Mitochondria, calcium and pro-apoptotic proteins as mediators in cell death signaling
Provedor de dados: 56
Autores: Smaili,S.S.; Hsu,Y.-T.; Carvalho,A.C.P.; Rosenstock,T.R.; Sharpe,J.C.; Youle,R.J.
Cellular Ca2+ signals are crucial in the control of most physiological processes, cell injury and programmed cell death through the regulation of a number of Ca2+-dependent enzymes such as phospholipases, proteases, and nucleases. Mitochondria along with the endoplasmic reticulum play pivotal roles in regulating intracellular Ca2+ content. Mitochondria are endowed with multiple Ca2+ transport mechanisms by which they take up and release Ca2+ across their inner membrane. During cellular Ca2+ overload, mitochondria take up cytosolic Ca2+, which in turn induces opening of permeability transition pores and disrupts the mitochondrial membrane potential (&lt;FONT FACE=Symbol&gt;Dy&lt;/FONT&gt;m). The collapse of &lt;FONT FACE=Symbol&gt;Dy&lt;/FONT&gt;m along...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ca2+; Mitochondrial Ca2+ uptake; Mitochondrial Ca2+ efflux; Permeability transition; Apoptosis; Bcl-2 family; Bax and apoptosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200004

Liver lipid peroxidation and antioxidant capacity in cerulein-induced acute pancreatitis
Provedor de dados: 56
Autores: Batcioglu,K.; Gul,M.; Uyumlu,A.B.; Esrefoglu,M.
The aim of this study was to evaluate the role of oxidative damage in pancreatitis-induced hepatic injury. Thirty-five rats were divided into five groups (each of 7 rats): control, cerulein (100 µg/kg body weight), cerulein and pentoxifylline (12 mg/kg body weight), cerulein plus L-NAME (10 mg/kg body weight) and cerulein plus L-arginine (160 mg/kg body weight). The degree of hepatic cell degeneration differed significantly between groups. Mean malondialdehyde levels were 7.00 ± 2.29, 20.89 ± 10.13, 11.52 ± 4.60, 18.69 ± 8.56, and 8.58 ± 3.68 nmol/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively. Mean catalase activity was 3.20 ± 0.83, 1.09 ± 0.35, 2.05 ± 0.91, 1.70 ± 0.60, and 2.85 ± 0.47 U/mg protein for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute pancreatitis; Liver injury; Oxidative damage; Pentoxifylline; L-NAME; L-arginine.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900001

Excavation of attractor modules for nasopharyngeal carcinoma via integrating systemic module inference with attract method
Provedor de dados: 56
Autores: Jiang,T.; Jiang,C.-Y.; Shu,J.-H.; Xu,Y.-J.
The molecular mechanism of nasopharyngeal carcinoma (NPC) is poorly understood and effective therapeutic approaches are needed. This research aimed to excavate the attractor modules involved in the progression of NPC and provide further understanding of the underlying mechanism of NPC. Based on the gene expression data of NPC, two specific protein-protein interaction networks for NPC and control conditions were re-weighted using Pearson correlation coefficient. Then, a systematic tracking of candidate modules was conducted on the re-weighted networks via cliques algorithm, and a total of 19 and 38 modules were separately identified from NPC and control networks, respectively. Among them, 8 pairs of modules with similar gene composition were selected, and 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nasopharyngeal carcinoma; Protein-protein interaction; Attract; Module; Cell division.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800602

Advances in biliary atresia: from patient care to research
Provedor de dados: 56
Autores: Santos,J.L.; Carvalho,E.; Bezerra,J.A.
Biliary atresia, the most common cause of liver transplantation in children, remains a challenge for clinicians and investigators. The development of new therapeutic options, besides the typical hepatoportoenterostomy, depends on a greater understanding of its pathogenesis and how it relates to the clinical phenotypes at diagnosis and the rate of disease progression. In this review, we present a perspective of how recent research has advanced the understanding of the disease and has improved clinical care protocols. Molecular and morphological analyses at diagnosis point to the potential contributions of polymorphism in the CFC1 and VEGF genes to the pathogenesis of the disease, and to an association between the degree of bile duct proliferation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biliary atresia; Etiology; Prognosis; Therapeutics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600001

Gender differences in serum CK-MB mass levels in healthy Braziliansubjects
Provedor de dados: 56
Autores: Araki,L.M.; Nogueira,A.A.R.; Mansur,A.P.
The creatine kinase-isoenzyme MB (CK-MB) mass assay is one of the laboratory tests used for the diagnosis of myocardial infarction. It is recommended, however, that reference limits should take gender and race into account. In the present study, we analyzed the plasma CK-MB mass and troponin levels of 244 healthy volunteers without a personal history of coronary artery disease and with no chronic diseases, muscular trauma or hypothyroidism, and not taking statins. The tests were performed with commercial kits, CK-MB mass turbo kit and Troponin I turbo kit, using the Immulite 1000 analyzer from Siemens Healthcare Diagnostic. The values were separated according to gender and showed significant differences by the Mann-Whitney test. Mean (± SD) CK-MB mass...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CK-MB mass; Gender; Healthy volunteers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300010

Construction of a recombinant adenovirus co-expressing truncated human prostate-specific membrane antigen and mouse 4-1BBL genes and its effect on dendritic cells
Provedor de dados: 56
Autores: Weng,Xiaodong; Kuang,Youlin; Liu,Xiuheng; Chen,Zhiyuan; Zhu,Hengcheng; Chen,Hui; Jiang,Botao; Shen,Hao.
Our aim was to construct a recombinant adenovirus co-expressing truncated human prostate-specific membrane antigen (tPSMA) and mouse 4-1BBL genes and to determine its effect on dendritic cells (DCs) generated from bone marrow suspensions harvested from C57BL/6 mice for which the effect of 4-1BBL on DCs is not clear, especially during DCs processing tumor-associated antigen. Replication deficient adenovirus AdMaxTM Expression System was used to construct recombinant adenovirus Ad-tPSMA-internal ribosome entry site-mouse 4-1BBL (Ad-tPSMA-IRES-m4-1BBL) and Ad-enhanced green fluorescent protein. Day 7 proliferating DC aggregates generated from C57BL/6 mice were collected as immature DCs and further mature DCs were obtained by lipopolysaccharide activated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; PSMA; Co-stimulatory molecule; 4-1BBL; Adenovirus; Dendritic cells.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300002

HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
Provedor de dados: 56
Autores: Rossini,M.A.A.; Diaz,R.S.; Caseiro,M.; Turcato,G.; Accetturi,C.A.; Sabino,E.C.
In order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility analysis. Of the 95 samples, 12 (12.6%) were PCR negative (6 samples from each group); low DNA concentration was the reason for non-amplification in half of these cases. Of the 42 typed cases from São Paulo, 34 (81%, 95% confidence limits 74.9 to 87.0%) were B and 8 (19%, 95% confidence limits 12.9 to 25.0%) were F, whereas of the 41 typed cases from Santos, 39 (95%, 95% confidence limits 91.6 to 98.4%)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HIV-1 subtypes; Intravenous drug users; Molecular epidemiology; Heteroduplex mobility analysis; Brazil.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100005

Impact of acute exposure to air pollution on the cardiorespiratory performance of military firemen
Provedor de dados: 56
Autores: Oliveira,R.S.; Barros Neto,T.L.; Braga,A.L.F.; Raso,V.; Pereira,L.A.A.; Morette,S.R.; Carneiro,R.C.
The objective of the present study was to determine the impact of acute short-term exposure to air pollution on the cardiorespiratory performance of military fireman living and working in the city of Guarujá, São Paulo, Brazil. Twenty-five healthy non-smoking firemen aged 24 to 45 years had about 1 h of exposure to low and high levels of air pollution. The tests consisted of two phases: phase A, in Bertioga, a town with low levels of air pollution, and phase B, in Cubatão, a polluted town, with a 7-day interval between phases. The volunteers remained in the cities (Bertioga/Cubatão) only for the time required to perform the tests. Cumulative load 10 ± 2 min-long exertion tests were performed on a treadmill, consisting of a 2-min stage at a load of 7 km/h,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Air pollution; Firemen; Cardiorespiratory performance; Cardiopulmonary exercise testing.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200016

Functional and morphological effects of resistance exercise on disuse-induced skeletal muscle atrophy
Provedor de dados: 56
Autores: Nicastro,H.; Zanchi,N.E.; da Luz,C.R.; Lancha Jr.,A.H.
Abstract The reduction of skeletal muscle loss in pathological states, such as muscle disuse, has considerable effects in terms of rehabilitation and quality of life. Since there is no currently effective and safe treatment available for skeletal muscle atrophy, the search for new alternatives is necessary. Resistance exercise (RE) seems to be an important tool in the treatment of disuse-induced skeletal muscle atrophy by promoting positive functional (strength and power) and structural (hypertrophy and phenotypic changes) adaptive responses. Human and animal studies using different types of resistance exercise (flywheel, vascular occlusion, dynamic, isometric, and eccentric) have obtained results of great importance. However, since RE is a complex...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Resistance exercise; Vascular occlusion; Eccentric exercise; Disuse; Atrophy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100001

Experimental model of heterotopic ossification in Wistar rats
Provedor de dados: 56
Autores: Zotz,T.G.G.; Paula,J.B. de; Moser,A.D.L.
Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues adjacent to large joints, resulting in joint mobility deficit. In order to determine which treatment techniques are more appropriate for such condition, experimental models of induced heterotopic bone formation have been proposed using heterologous demineralized bone matrix implants and bone morphogenetic protein and other tissues. The objective of the present experimental study was to identify a reliable protocol to induce HO in Wistar rats, based on autologous bone marrow (BM) implantation, comparing 3 different BM volumes and based on literature evidence of this HO induction model in larger laboratory animals. Twelve male Wistar albino...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heterotopic ossification; Autologous bone marrow; Serum alkaline phosphatase.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600004

Duodenal-jejunal bypass normalizes pancreatic islet proliferation rate and function but not hepatic steatosis in hypothalamic obese rats
Provedor de dados: 56
Autores: Cantelli,K.R.; Soares,G.M.; Ribeiro,R.A.; Balbo,S.L.; Lubaczeuski,C.; Boschero,A.C.; Araújo,A.C.F.; Bonfleur,M.L.
Modifications in life-style and/or pharmacotherapies contribute to weight loss and ameliorate the metabolic profile of diet-induced obese humans and rodents. Since these strategies fail to treat hypothalamic obesity, we have assessed the possible mechanisms by which duodenal-jejunal bypass (DJB) surgery regulates hepatic lipid metabolism and the morphophysiology of pancreatic islets, in hypothalamic obese (HyO) rats. During the first 5 days of life, male Wistar rats received subcutaneous injections of monosodium glutamate (4 g/kg body weight, HyO group), or saline (CTL). At 90 days of age, HyO rats were randomly subjected to DJB (HyO DJB group) or sham surgery (HyO Sham group). HyO Sham rats were morbidly obese, insulin resistant, hypertriglyceridemic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Duodenal-jejunal bypass; Hepatic fatty acid metabolism; Hypothalamic obesity; Ki67; Insulin secretion.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500601

A novel polar-based human face recognition computational model
Provedor de dados: 56
Autores: Zana,Y.; Mena-Chalco,J.P.; Cesar Jr.,R.M.
Motivated by a recently proposed biologically inspired face recognition approach, we investigated the relation between human behavior and a computational model based on Fourier-Bessel (FB) spatial patterns. We measured human recognition performance of FB filtered face images using an 8-alternative forced-choice method. Test stimuli were generated by converting the images from the spatial to the FB domain, filtering the resulting coefficients with a band-pass filter, and finally taking the inverse FB transformation of the filtered coefficients. The performance of the computational models was tested using a simulation of the psychophysical experiment. In the FB model, face images were first filtered by simulated V1- type neurons and later analyzed globally...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Face processing; Visual perception; Cognitive processes; Computational modeling.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700008

Michel Abu Jamra (1916-1999)
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/other
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200001

Presence of age- and sex-related differences in heart rate variability despite the maintenance of a suitable level of accelerometer-based physical activity
Provedor de dados: 56
Autores: Spina,G.D.; Gonze,B.B.; Barbosa,A.C.B.; Sperandio,E.F.; Dourado,V.Z.
There is currently a lack of information adjacent on the influence of sex and age in heart rate variability (HRV), adjusted according to accelerometer-based physical activity (PADL). We hypothesized that the effect of sex and age on the HRV should be reduced or absent in individuals with a suitable PADL level. We aim to evaluate the influence of sex and age on HRV, adjusted for the confounding effects of the PADL level. A total of 485 age-stratified subjects (18–39, 40–59, and ≥60 years) underwent HRV analyses at rest and 7-day assessments of accelerometer-based PADL. Multivariate analyses of covariance were done using log-transformed HRV indices as outcomes, age and sex as fixed factors, and PADL, cardiovascular risk, fat body mass, and heart rate (HR) at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autonomic balance; HRV; Aging.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800605

Genetic potential for an acute inflammatory response in IgA glomerulonephritis in mice
Provedor de dados: 56
Autores: Kurihara,R.S.; Yokoo,M.; Domingues,W.V.; Cabrera,W.H.; Ribeiro,O.G.; Ibañez,O.M.; Malheiros,D.A.; Barros,R.T.; de Almeida Prado,E.B.
Mice selected on the basis of an acute inflammatory response (AIR) can provide information about the immunopathological mechanisms of glomerulonephritis. We studied the differences between mice selected for a maximal AIR (AIRmax that attract more polymorphonuclear cells to the site of injury) or a minimal AIR (AIRmin that attract more mononuclear cells) in an experimental model of IgA nephropathy in order to investigate the effect of genetic background on glomerular disease progression and the participation of the monocyte chemoattractant protein-1 (MCP-1) chemokine. IgA nephropathy was induced by intraperitoneal ovalbumin injection and bile duct ligation in AIRmax and AIRmin mice. Histological changes, urinary protein/creatinine ratio, serum IgA levels,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monocyte chemoattractant protein-1; IgA; Glomerulonephritis; Acute inflammatory response; Chemokine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200009

Therapy of tacrolimus combined with corticosteroids in idiopathic membranous nephropathy
Provedor de dados: 56
Autores: Cui,W.; Lu,X.; Min,X.; Liu,M.; Guan,S.; Wang,Y.; Luo,M.; Li,W.; Li,Q.; Dong,W.; Miao,L.; Luo,P.
We evaluated the efficacy and safety of tacrolimus (TAC) combined with corticosteroids in treating patients with idiopathic membranous nephropathy (IMN). One hundred seventy-seven biopsy-proven IMN patients were recruited in this retrospective clinical study. Sixty patients received TAC (target blood concentration of 4–8 ng/mL) and 117 patients received daily cyclophosphamide (CYC, 100 mg) combined with prednisone. Remission rates at the end of the first, second and third month in the TAC group were significantly higher than that in the CYC group (1st: 35.0 vs 19.7%, P&lt;0.05; 2nd: 56.7 vs 38.5%, P&lt;0.05; 3rd: 76.7 vs 59.0%, P&lt;0.05). In the first 3 months, daily urinary protein and serum albumin in the TAC group obtained a better improvement than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclophosphamide; Efficacy; Idiopathic membranous nephropathy; Retrospective clinical study; Tacrolimus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400703

The validity and 4-year test-retest reliability of the Brazilian version of the Eating Attitudes Test-26
Provedor de dados: 56
Autores: Nunes,M.A.; Camey,S.; Olinto,M.T.A.; Mari,J.J.
In a cross-sectional study conducted four years ago to assess the validity of the Brazilian version of the Eating Attitudes Test-26 (EAT-26) for the identification of abnormal eating behaviors in a population of young females in Southern Brazil, 56 women presented abnormal eating behavior as indicated by the EAT-26 and the Edinburgh Bulimic Investigation Test. They were each matched for age and neighborhood to two normal controls (N = 112) and were re-assessed four years later with the two screening questionnaires plus the Composite International Diagnostic Interview (CIDI). The EAT results were then compared to diagnoses originating from the CIDI. To evaluate the temporal stability of the two screening questionnaires, a test-retest design was applied to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Validity coefficients; Eating Attitudes Test-26 (EAT-26); Abnormal eating behavior; Eating disorders; Anorexia; Bulimia.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100013

Bone mineral density of Brazilian girls with juvenile dermatomyositis
Provedor de dados: 56
Autores: Castro,T.C.M. de; Terreri,M.T.R.A.; Szejnfeld,V.L.; Len,C.; Fonseca,A.S.M. da; Hilário,M.O.E.
We measured bone mineral density (BMD) in girls with juvenile dermatomyositis (JDM) considering multiple factors in order to determine if it could be used as a predictor of reduction in bone mass. A cross-sectional study of lumbar spine BMD (L2-L4) was conducted on 10 girls aged 7-16 years with JDM. A group of 20 age-matched healthy girls was used as control. Lumbar spine BMD was measured by dual-energy X-ray absorptiometry. Weight, height and pubertal Tanner stage were determined in all patients and controls. Duration of disease and mean daily and cumulative steroid doses were calculated for all patients on the basis of their medical charts. JDM activity was determined on the basis of the presence of muscle weakness, cutaneous vasculitis and/or elevation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatomyositis; Adolescent girls; Bone mineral density; Osteoporosis; Corticosteroids.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200020

Predictors of early treatment discontinuation and severe anemia in a Brazilian cohort of hepatitis C patients treated with first-generation protease inhibitors
Provedor de dados: 56
Autores: Miotto,N.; Mendes,L.C.; Zanaga,L.P.; Goncales,E.S.L.; Lazarini,M.S.K.; Pedro,M.N.; Goncales Jr.,F.L.; Stucchi,R.S.B.; Vigani,A.G.
The aim of this study was to determine risk factors for adverse events (AE)-related treatment discontinuation and severe anemia among patients with chronic hepatitis C virus (HCV) genotype 1 infection, treated with first-generation protease inhibitor (PI)-based therapy. We included all patients who initiated treatment with PI-based therapy at a Brazilian university hospital between November 2013 and December 2014. We prospectively collected data from medical records using standardized questionnaires and used Epi Info 6.0 for analysis. Severe anemia was defined as hemoglobin ≤8.5 mg/dL. We included 203 patients: 132 treated with telaprevir (TVR) and 71 treated with boceprevir (BOC). AE-related treatment discontinuation rate was 19.2% and anemia was the main...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C treatment; Protease inhibitor; Anemia; Adverse events; Treatment interruption.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700702

Antinociception induced by stimulating amygdaloid nuclei in rats: changes produced by systemically administered antagonists
Provedor de dados: 56
Autores: Oliveira,M.A.; Prado,W.A.
The antinociceptive effects of stimulating the medial (ME) and central (CE) nuclei of the amygdala in rats were evaluated by the changes in the latency for the tail withdrawal reflex to noxious heating of the skin. A 30-s period of sine-wave stimulation of the ME or CE produced a significant and short increase in the duration of tail flick latency. A 15-s period of stimulation was ineffective. Repeated stimulation of these nuclei at 48-h intervals produced progressively smaller effects. The antinociception evoked from the ME was significantly reduced by the previous systemic administration of naloxone, methysergide, atropine, phenoxybenzamine, and propranolol, but not by mecamylamine, all given at the dose of 1.0 mg/kg. Previous systemic administration of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antinociception; Amygdala; Medial nucleus of the amygdala; Central nucleus of the amygdala; Tail flick test; Stimulation-produced antinociception.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500013

Cardiovascular and respiratory responses to microinjection of L-glutamate into the caudal pressor area in conscious and anesthetized rats
Provedor de dados: 56
Autores: Silva,N.F.; Pires,J.G.P.; Campos,R.R.; Futuro Neto,H.A.
The role of the caudal pressor area (CPA) in the maintenance of vasomotor tonus in anesthetized and decerebrate animals has been clearly established. In conscious animals, however, the participation of CPA in the cardiovascular control remains to be fully elucidated. In the present study, unilateral L-glutamate (L-Glu) (10 and/or 20 nmol/70 nl) microinjection into CPA, in conscious male Wistar rats (250-280 g) caused a significant increase in mean arterial blood pressure (MAP; control: 112 ± 1.9 mmHg; after 20 nmol L-Glu: 139 ± 4.5 mmHg, N = 12, P&lt;0.05) and respiratory rate (control: 81 ± 3.5 breaths/min; after 10 nmol L-Glu: 92 ± 3 breaths/min, P&lt;0.05; after 20 nmol L-Glu: 104 ± 5 breaths/min, N = 6, P&lt;0.05). The subsequent anesthesia with...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Caudal pressor area; Ventrolateral medulla; Blood pressure; L-glutamate; Respiration.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200013

Dose-related effects of propericiazine in rats
Provedor de dados: 56
Autores: Cechin,E.M.; Quevedo,J.; Barichello,T.; Machado-Vieira,R.; Gentil,V.; Kapczinski,F.
We evaluated the effects of the neuroleptic agent propericiazine on animal models of anxiety and memory. Adult male Wistar rats (250 to 350 g) received intraperitoneal injections of propericiazine (0.05, 0.075 and 0.1 mg/kg), diazepam (1 mg/kg), saline, or diazepam vehicle (20% propylene glycol and 80% saline) 30 min prior to the experimental procedure. Animals (10-15 for each task) were tested for step-down inhibitory avoidance (0.3-mA footshock) and habituation to an open-field for memory assessment, and submitted to the elevated plus-maze to evaluate the effects of propericiazine in a model of anxiety. Animals treated with 0.075 mg/kg propericiazine showed a reduction in anxiety measures (P&lt;0.05) similar to that observed in those treated with...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Propericiazine; Memory; Anxiety; Diazepam; Elevated plus-maze.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200010

Effects of piperitenone oxide on the intestinal smooth muscle of the guinea pig
Provedor de dados: 56
We investigated the effects of piperitenone oxide (PO), a major constituent of the essential oil of Mentha x villosa, on the guinea pig ileum. PO (30 to 740 µg/ml) relaxed basal tonus without significantly altering the resting membrane potential. In addition, PO relaxed preparations precontracted with either 60 mM K+ or 5 mM tetraethylammonium in a concentration-dependent manner. At concentrations from 0.1 to 10 µg/ml PO potentiated acetylcholine-induced contractions, while higher concentrations (&amp;gt;30 µg/ml) blocked this response. These higher PO concentrations also inhibited contractions induced by 60 mM K+. PO also blocked the components of acetylcholine contraction which are not sensitive to nifedipine or to solutions with nominal zero Ca2+ and...
Palavras-chave: Piperitenone oxide; Mentha x villosa; Ileum; Essential oil; Muscle relaxation; Acetylcholine; K+-induced contraction.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600014

Protective roles of pulmonary rehabilitation mixture in experimental pulmonary fibrosis in vitro and in vivo
Provedor de dados: 56
Autores: Zhang,L.; Ji,Y.X.; Jiang,W.L.; Lv,C.J.
Abnormal high mobility group protein B1 (HMGB1) activation is involved in the pathogenesis of pulmonary fibrosis. Pulmonary rehabilitation mixture (PRM), which combines extracts from eight traditional Chinese medicines, has very good lung protection in clinical use. However, it is not known if PRM has anti-fibrotic activity. In this study, we investigated the effects of PRM on transforming growth factor-β1 (TGF-β1)-mediated and bleomycin (BLM)-induced pulmonary fibrosis in vitro and in vivo. The effects of PRM on TGF-β1-mediated epithelial-mesenchymal transition (EMT) in A549 cells, on the proliferation of human lung fibroblasts (HLF-1) in vitro, and on BLM-induced pulmonary fibrosis in vivo were investigated. PRM treatment resulted in a reduction of EMT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High mobility group protein B1; Epithelial-mesenchymal transition; Pulmonary rehabilitation mixture; Receptor for advanced glycation end-product.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600545

Distribution of liposome-encapsulated antimony in dogs
Provedor de dados: 56
Autores: Schettini,D.A.; Costa Val,A.P.; Souza,L.F.; Demicheli,C.; Rocha,O.G.F.; Melo,M.N.; Michalick,M.S.M.; Frézard,F.
The achievement of complete cure in dogs with visceral leishmaniasis is currently a great challenge, since dogs are the main reservoir for the transmission of visceral leishmaniasis to humans and they respond poorly to conventional treatment with pentavalent antimonials. In order to improve the efficacy of treatment, we developed a novel formulation for meglumine antimoniate based on the encapsulation of this drug in freeze-dried liposomes (LMA). The aim of the present study was to evaluate the biodistribution of antimony (Sb) in dogs following a single intravenous bolus injection of LMA. Four healthy male mongrel dogs received LMA at 3.8 mg Sb/kg body weight and were sacrificed 3, 48 and 96 h and 7 days later. Antimony was determined in the blood, liver,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Liposomes; Meglumine antimoniate; Biodistribution; Leishmaniasis; Dogs.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200015

Use of inflammatory molecules to predict the occurrence of fever in onco-hematological patients with neutropenia
Provedor de dados: 56
Autores: Ribeiro,A.F. Tibúrcio; Nobre,V.; Neuenschwander,L.C.; Teixeira,A.L.; Xavier,S.G.; Paula,F.D.F.; Teixeira,M.M.; Teixeira,J.C.A.; Bittencourt,H.
Febrile neutropenia remains a frequent complication in onco-hematological patients, and changes in the circulating level of inflammatory molecules (IM) may precede the occurrence of fever. The present observational prospective study was carried out to evaluate the behavior of plasma tumor necrosis factor alpha (TNF-α), soluble TNF-α I and II receptors (sTNFRI and sTNFRII), monocyte chemoattractant protein-1 [MCP-1 or chemokine (c-c motif) ligand 2 (CCL2)], macrophage inflammatory protein-1α (MIP-1α or CCL3), eotaxin (CCL11), interleukin-8 (IL-8 or CXCL8), and interferon-inducible protein-10 (IP-10 or CXCL10) in 32 episodes of neutropenia in 26 onco-hematological patients. IM were tested on enrollment and 24-48 h before the onset of fever and within 24 h of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutropenia; Cytokines; Oncological hematology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200200

Frequency of Fanconi anemia in Brazil and efficacy of screening for the FANCA 3788-3790del mutation
Provedor de dados: 56
Autores: Magdalena,N.; Pilonetto,D.V.; Bitencourt,M.A.; Pereira,N.F.; Ribeiro,R.C.; Jeng,M.; Pasquini,R.
Fanconi anemia (FA) is an autosomal recessive genetic disease characterized by progressive bone marrow failure, susceptibility to cancer and multiple congenital anomalies. There is important clinical variability among patients and the knowledge of factors which might predict outcome would greatly help the decision making regarding the choices of treatment and the appropriate time to start it. Future studies of the possible correlation between specific mutations with specific clinical presentations will provide the answer to one of these factors. At our Center we standardized a rapid and precise screening test using a mismatch PCR assay for a specific mutation (3788-3790del in exon 38 of gene FANCA) in Brazilian FA patients. We present the results obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fanconi anemia; FANCA; 3788-3790del mutation; Genetic screening.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500003

Propofol attenuates sepsis-induced acute kidney injury by regulating miR-290-5p/CCL-2 signaling pathway
Provedor de dados: 56
Autores: Zheng,Guodong; Qu,Hong; Li,Fen; Ma,Weiquan; Yang,Hong.
Previous studies have indicated that propofol has immunomodulatory and antioxidative properties. However, the renoprotection effect and the precise mechanisms of propofol in sepsis-induced renal injury remain unclear. The purpose of the present study was to investigate the role of miR-290-5p/CCL-2 signaling in septic mice treatment with propofol. Mice were treated with propofol (50 mg/kg) twice within 24 h. Survival outcome was monitored within 48 h. The mRNA and protein levels were assayed by qRT-PCR and western blotting, respectively. Mouse podocytes (MPC5) were treated with lipopolysaccharide (LPS) to establish the cell model in vitro. The proliferation of MPC5 was monitored using the MTS assay. Cell apoptosis was analyzed by flow cytometry. Propofol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propofol; Sepsis; MiR-290-5p; CCL-2; Acute kidney injury.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100612

Association of CYP7A1 -278A&gt;C polymorphism and the response of plasma triglyceride after dietary intervention in dyslipidemic patients
Provedor de dados: 56
Autores: Barcelos,A.L.V.; Chies,R.; Almeida,S.E.M.; Fiegenbaum,M.; Schweigert,I.D.; Chula,F.G.L.; Rossetti,M.L.; Silva,C.M.D.
We investigated the effect of the -278A&gt;C polymorphism in the CYP7A1 gene on the response of plasma lipids to a reduced-fat diet for 6 to 8 weeks in a group of 82 dyslipidemic males with a mean age of 46.0 ± 11.7 years. Individuals who presented at least one high alteration in total cholesterol, low-density lipoprotein cholesterol or triglyceride values were considered to be dyslipidemic. Exclusion criteria were secondary dyslipidemia due to diabetes mellitus, renal, liver, or thyroid disease. None of the subjects were using lipid-lowering medication. Baseline and follow-up lipid concentrations were measured. The genotypes were determined by the digestion of PCR products with the BsaI restriction endonuclease. There were statistically significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dyslipidemia; Polymorphisms; Diet; CYP7A1; Southern Brazil.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600003

Effect of risperidone on proliferation and apoptosis of MC3T3-E1 cells
Provedor de dados: 56
Autores: Zheng,Lei; Yang,Lixia; Zhao,Xin; Long,Niya; Li,Peifan; Wang,Yiming.
This aim of this study was to assess the molecular mechanism of osteoporosis in schizophrenia patients with risperidone use. Here, we investigated the effects of risperidone on cellular proliferation and apoptosis of a preosteoblast cell line, MC3T3-E1. Cell viability and apoptotic rate of MC3T3-E1 were detected by cell counting kit-8 and flow cytometry at a serial dose of risperidone and at different time points, respectively. Bone transformation relevant gene serum osteocalcin (BGP), collagen 1, tumor necrosis factor-α (TNF-α), osteoprotegerin (OPG), and receptor activator of nuclear factor-κB ligand (RANKL) mRNA levels were determined by real-time PCR (qPCR). Their protein expression patterns were evaluated using western blot. The results revealed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Risperidone; MC3T3-E1 cells; Collagen 1; Tumor necrosis factor-α; Receptor activator of nuclear factor-κB ligand.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300605

Metabolic markers following beta-adrenoceptor agonist infusion in footshock-stressed rats
Provedor de dados: 56
Autores: Verago,J.L.; Grassi-Kassisse,D.M.; Spadari-Bratfisch,R.C.
Stress hormones can alter metabolic functions in adipose tissue and liver, as well as the sensitivity of rat white adipocytes and rat atrial responses to ß-adrenergic agonists. In this study, we examined the effects of three daily footshock stress sessions on the plasma corticosterone, glucose, glycerol and triacylglycerol levels of fed, conscious male rats, and on the plasma glucose, glycerol and triacylglycerol levels of the same rats following iv infusions of ß-adrenergic agonists (isoproterenol: 0.4 nmol kg-1 min-1, noradrenaline: 5.0 µg kg-1 day-1, and BRL 37344 ([±]-[4-(2-[(2-[3-chlorophenyl]-2-hydroxyethyl)amino]propyl)phenoxy]acetic acid), a selective ß3-adrenoceptor agonist: 0.4 nmol kg-1 min-1). Plasma corticosterone levels increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucose; Glycerol; Triacylglycerol; Triglycerides; Beta-adrenergic agonist; Corticosterone; Infusion.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900014

Collaborative care model improves self-care ability, quality of life and cardiac function of patients with chronic heart failure
Provedor de dados: 56
Autores: Hua,C.Y.; Huang,Y.; Su,Y.H.; Bu,J.Y.; Tao,H.M.
Chronic heart failure (CHF) is a common chronic disease that requires much care. This study aimed to explore the effects of collaborative care model (CCM) on patients with CHF. A total of 114 CHF patients were enrolled in this study, and were randomly and equally divided into two groups: control and experimental. Patients in the two groups received either usual care or CCM for 3 continuous months. The impacts of CCM on the self-care ability and quality of life were assessed using self-care of heart failure index and short form health survey 12, respectively. Further, cardiac function was assessed by measuring left ventricular ejection fraction (LVEF) and the level of N-terminal pro-B-type natriuretic peptide (NT-proBNP), and by the 6-min walking test....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collaborative care model; Chronic heart failure; Self-care; Quality of life; Cardiac function.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100609

Reference value of brachial-ankle pulse wave velocity for the eastern Chinese population and potential influencing factors
Provedor de dados: 56
Autores: Zi-Sheng,Ai; Jue,Li; Zhong-Min,Liu; Hui-Min,Fan; Zhang,Dai-Fu; Yun,Zhu; Zhang,Li-Juan; Zhu,Wen-Qing; Bao,Yan.
The present study was conducted to obtain reference values for brachial-ankle pulse wave velocity (baPWV) and to evaluate influencing factors of baPWV according to gender. Using automatic devices, baPWV was measured simultaneously in 2095 subjects. A total of 647 healthy subjects, none of whom presented atherosclerotic risk factors, were analyzed in the present study. Two different statistical methods were used to obtain reference values for baPWV according to subject gender and age. The association between baPWV value and gender, as well as other features, were analyzed. For male subjects, multiple stepwise analysis showed that age, systolic blood pressure (SBP), heart rate (HR), and plasma levels of triglycerides (TG) were independent predictors of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulse wave velocity; Reference value; Age; Gender; Influencing factor.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000004

Solubilization of Na,K-ATPase from rabbit kidney outer medulla using only C12E8
Provedor de dados: 56
Autores: Santos,H.L.; Lamas,R.P.; Ciancaglini,P.
SDS, C12E8, CHAPS or CHAPSO or a combination of two of these detergents is generally used for the solubilization of Na,K-ATPase and other ATPases. Our method using only C12E8 has the advantage of considerable reduction of the time for enzyme purification, with rapid solubilization and purification in a single chromatographic step. Na,K-ATPase-rich membrane fragments of rabbit kidney outer medulla were obtained without adding SDS. Optimum conditions for solubilization were obtained at 4ºC after rapid mixing of 1 mg of membrane Na,K-ATPase with 1 mg of C12E8/ml, yielding 98% recovery of the activity. The solubilized enzyme was purified by gel filtration on a Sepharose 6B column at 4ºC. Non-denaturing PAGE revealed a single protein band with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Na; K-ATPase Rabbit kidney medulla Membrane solubilization C12E8; (alphaß)2 Dimer.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300002

Challenges in the research and development of new human vaccines
Provedor de dados: 56
Autores: Barbosa,T.; Barral-Netto,M.
The field of vaccinology was born from the observations by the fathers of vaccination, Edward Jenner and Louis Pasteur, that a permanent, positive change in the way our bodies respond to life-threatening infectious diseases can be obtained by specific challenge with the inactivated infectious agent performed in a controlled manner, avoiding the development of clinical disease upon exposure to the virulent pathogen. Many of the vaccines still in use today were developed on an empirical basis, essentially following the paradigm established by Pasteur, “isolate, inactivate, and inject” the disease-causing microorganism, and are capable of eliciting uniform, long-term immune memory responses that constitute the key to their proven efficacy. However, vaccines...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human vaccines; Vaccine issues; Vaccine efficacy; Immunization strategies.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200103

Cannabidiol, a Cannabis sativa constituent, as an antipsychotic drug
Provedor de dados: 56
Autores: Zuardi,A.W.; Crippa,J.A.S.; Hallak,J.E.C.; Moreira,F.A.; Guimarães,F.S.
A high dose of delta9-tetrahydrocannabinol, the main Cannabis sativa (cannabis) component, induces anxiety and psychotic-like symptoms in healthy volunteers. These effects of delta9-tetrahydrocannabinol are significantly reduced by cannabidiol (CBD), a cannabis constituent which is devoid of the typical effects of the plant. This observation led us to suspect that CBD could have anxiolytic and/or antipsychotic actions. Studies in animal models and in healthy volunteers clearly suggest an anxiolytic-like effect of CBD. The antipsychotic-like properties of CBD have been investigated in animal models using behavioral and neurochemical techniques which suggested that CBD has a pharmacological profile similar to that of atypical antipsychotic drugs. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cannabidiol; D9-Tetrahydrocannabinol; Cannabinoid; Anxiety; Antipsychotic; Schizophrenia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400001

The role of autolysis loop in determining the specificity of coagulation proteases
Provedor de dados: 56
Autores: Yang,L.; Manithody,C.; Rezaie,A.R.
We recently demonstrated that the substitution of the autolysis loop (residues 143 to 154 in the chymotrypsin numbering system) of activated protein C (APC) with the corresponding loop of factor Xa (fXa) renders the APC mutant (APC/fX143-154) susceptible to inhibition by antithrombin (AT) in the presence of pentasaccharide. Our recent results further indicated, that in addition to an improvement in the reactivity of APC/fX143-154 with AT, both the amidolytic and anti-factor Va activities of the mutant APC have also been significantly increased. Since the autolysis loop of APC is five residues longer than the autolysis loop of fXa, it could not be ascertained whether this loop in the mutant APC specifically interacts with the activated conformation of AT or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Activated protein C; Factor Va; Antithrombin; Factor Xa; Serpins.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800005

Optimization of the RNeasy Mini Kit to obtain high-quality total RNA from sessile cells of Staphylococcus aureus
Provedor de dados: 56
Autores: Beltrame,C. O.; Côrtes,M. F.; Bandeira,P. T.; Figueiredo,A. M. S.
Biofilm formed by Staphylococcus aureus is considered an important virulence trait in the pathogenesis of infections associated with implantable medical devices. Gene expression analyses are important strategies for determining the mechanisms involved in production and regulation of biofilm. Obtaining intact RNA preparations is the first and most critical step for these studies. In this article, we describe an optimized protocol for obtaining total RNA from sessile cells of S. aureus using the RNeasy Mini Kit. This method essentially consists of a few steps, as follows: 1) addition of acetone-ethanol to sessile cells, 2) lysis with lysostaphin at 37°C/10 min, 3) vigorous mixing, 4) three cycles of freezing and thawing, and 5) purification of the lysate in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA preparation; MRSA; Biofilm; Sessile cells; Staphylococcus aureus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201071

Potential virulence of Klebsiella sp. isolates from enteral diets
Provedor de dados: 56
Autores: Pereira,S.C.L.; Vanetti,M.C.D.
We aimed to evaluate the potential virulence of Klebsiella isolates from enteral diets in hospitals, to support nosocomial infection control measures, especially among critical-care patients. Phenotypic determination of virulence factors, such as capsular expression on the external membrane, production of aerobactin siderophore, synthesis of capsular polysaccharide, hemolytic and phospholipase activity, and resistance to antibiotics, which are used therapeutically, were investigated in strains of Klebsiella pneumoniae and K. oxytoca. Modular industrialized enteral diets (30 samples) as used in two public hospitals were analyzed, and Klebsiella isolates were obtained from six (20%) of them. The hypermucoviscous phenotype was observed in one of the K....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella; Enteral diets; Pathogenicity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900782

Advancement in liver laparoscopic resection – development of a new surgical device
Provedor de dados: 56
Autores: Vavra,P.; Karnik,L.; Skrobankova,M.; Jurcikova,J.; Ihnat,P.; Zonca,P.; Peteja,M.; El-Gendi,A.; Czudek,S.
Liver resection is the standard treatment for any liver lesion. Laparoscopic liver resection is associated with lower intra-operative blood loss and fewer complications than open resection. Access to the posterior part of the right liver lobe is very uncomfortable and difficult for surgeons due the anatomic position, especially when employing laparoscopic surgery. Based on these experiences, a new laparoscopic device was developed that is capable of bending its long axis and allowing the application of radiofrequency energy in areas that were not technically accessible. The device is equipped with four telescopic needle electrodes that cause tissue coagulation after the delivery of radiofrequency energy. Ex vivo testing was performed in 2012 and 2014 at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laparoscopic surgery; Liver; Radiofrequency energy; Posterior liver segments.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400617

Normal range values for thromboelastography in healthy adult volunteers
Provedor de dados: 56
Autores: Scarpelini,S.; Rhind,S.G.; Nascimento,B.; Tien,H.; Shek,P.N.; Peng,H.T.; Huang,H.; Pinto,R.; Speers,V.; Reis,M.; Rizoli,S.B.
Thromboelastography (TEG®) provides a functional evaluation of coagulation. It has characteristics of an ideal coagulation test for trauma, but is not frequently used, partially due to lack of both standardized techniques and normal values. We determined normal values for our population, compared them to those of the manufacturer and evaluated the effect of gender, age, blood type, and ethnicity. The technique was standardized using citrated blood, kaolin and was performed on a Haemoscope 5000 device. Volunteers were interviewed and excluded if pregnant, on anticoagulants or having a bleeding disorder. The TEG® parameters analyzed were R, K, α, MA, LY30, and coagulation index. All volunteers outside the manufacturer’s normal range underwent extensive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulation; Thromboelastography; Normal values.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200015

An update on the tools for creating transgenic animal models of human diseases – focus on atherosclerosis
Provedor de dados: 56
Autores: Volobueva,A.S.; Orekhov,A.N.; Deykin,A.V.
Animal models of diseases are invaluable tools of modern medicine. More than forty years have passed since the first successful experiments and the spectrum of available models, as well as the list of methods for creating them, have expanded dramatically. The major step forward in creating specific disease models was the development of gene editing techniques, which allowed for targeted modification of the animal's genome. In this review, we discuss the available tools for creating transgenic animal models, such as transgenesis methods, recombinases, and nucleases, including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and CRISPR/Cas9 systems. We then focus specifically on the models of atherosclerosis, especially...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal models; Gene editing; Atherosclerosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500301

Evaluation of two 99mTc-DTPA radioaerosols with different characteristics in lung ventilation studies
Provedor de dados: 56
Autores: Silveira,C.M.; Castiglioni,M.L.V.; Torres,I.C.G.; Medeiros,R.B.; Jardim,J.R.
Two radioaerosol preparations, TechneScan®-DTPA (99mTc-DTPA, 40 mCi/3 ml; IPEN-CNEN, São Paulo, SP, Brazil) and TechneScan®-DTPA/AEROSOL (99mTc-DTPA/A, 15 mCi/1.5 ml with 0.5 ml ethanol; Mallinckrodt Medical, St. Louis, MO, USA), were compared in pulmonary ventilation studies in terms of total radiocounts and clearance after inhalation. An aerosol with ethanol is supposed to better distribute the radioparticles in the lungs. Twenty normal nonsmoking volunteers (10 men and 10 women), mean age of 23.2 years (range: 20 to 35 years), were studied. Images were obtained immediately and 30, 60 and 90 min after inhalation. Total and regional counts were obtained and the clearance half-lives of both lungs were determined. There was no difference in total counts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulmonary ventilation; Pulmonary clearance; Radioaerosol; 99mTc-DTPA.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000009

Specific alterations in plasma proteins during depressed, manic, and euthymic states of bipolar disorder
Provedor de dados: 56
Autores: Song,Y.R.; Wu,B.; Yang,Y.T.; Chen,J.; Zhang,L.J.; Zhang,Z.W.; Shi,H.Y.; Huang,C.L.; Pan,J.X.; Xie,P.
Bipolar disorder (BD) is a common psychiatric mood disorder affecting more than 1-2% of the general population of different European countries. Unfortunately, there is no objective laboratory-based test to aid BD diagnosis or monitor its progression, and little is known about the molecular basis of BD. Here, we performed a comparative proteomic study to identify differentially expressed plasma proteins in various BD mood states (depressed BD, manic BD, and euthymic BD) relative to healthy controls. A total of 10 euthymic BD, 20 depressed BD, 15 manic BD, and 20 demographically matched healthy control subjects were recruited. Seven high-abundance proteins were immunodepleted in plasma samples from the 4 experimental groups, which were then subjected to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bipolar disorder; Plasma; Proteomic; Carbonic anhydrase 1; Apolipoprotein.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001100973

Calcium antagonism and the vasorelaxation of the rat aorta induced by rotundifolone
Provedor de dados: 56
Autores: Guedes,D.N.; Silva,D.F.; Barbosa-Filho,J.M.; Medeiros,I.A.
The vasorelaxing activity of rotundifolone (ROT), a major constituent (63.5%) of the essential oil of Mentha x villosa, was tested in male Wistar rats (300-350 g). In isolated rat aortic rings, increasing ROT concentrations (0.3, 1, 10, 100, 300, and 500 µg/ml) inhibited the contractile effects of 1 µM phenylephrine and of 80 or 30 mM KCl (IC50 values, reported as means ± SEM = 184 ± 6, 185 ± 3 and 188 ± 19 µg/ml, N = 6, respectively). In aortic rings pre-contracted with 1 µM phenylephrine, the smooth muscle-relaxant activity of ROT was inhibited by removal of the vascular endothelium (IC50 value = 235 ± 7 µg/ml, N = 6). Furthermore, ROT inhibited (pD2 = 6.04, N = 6) the CaCl2-induced contraction in depolarizing medium in a concentration-dependent manner....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ca2+ mobilization; Rat aortic rings; Rotundifolone; Vasorelaxation; Mentha x villosa.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200014

Effects of a physical fitness program on memory and blood viscosity in sedentary elderly men
Provedor de dados: 56
Autores: Antunes,H.K.; De Mello,M.T.; Santos-Galduróz,R.F.; Galduróz,J.C.F.; Lemos,V.Aquino; Tufik,S.; Bueno,O.F.A.
The aim of this study was to investigate the effects of a 6-month exercise program on cognitive function and blood viscosity in sedentary elderly men. Forty-six healthy inactive men, aged 60–75 years were randomly distributed into a control group (n=23) and an experimental group (n=23). Participants underwent blood analysis and physical and memory evaluation, before and after the 6-month program of physical exercise. The control group was instructed not to alter its everyday activities; the experimental group took part in the fitness program. The program was conducted using a cycle ergometer, 3 times per week on alternate days, with intensity and volume individualized at ventilatory threshold 1. Sessions were continuous and maximum duration was 60 min...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cognition; Elderly adults; Physical exercise.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900805

Cyclosporin inhibits hyperalgesia and edema in arthritic rats: role of the central nervous system
Provedor de dados: 56
Since arthritis induced by Mycobacterium products (adjuvant) in rats is considered to be immunologically driven, the objective of the present study was to determine if the immunosuppressor drug cyclosporin could affect hindpaw edema and joint hyperalgesia simultaneously. Female Holtzman rats (140-170 g) presented hyperalgesia and edema on the 8th and 12th day following adjuvant injection. Daily systemic (oral or intramuscular) administration of cyclosporin (0.5-5.0 mg kg-1 day-1) or dexamethasone (0.01-0.1 mg kg-1 day-1) for 15 days starting on day zero dose-dependently inhibited the hindpaw edema and hyperalgesia in arthritic rats. However, hyperalgesia but not edema could be detected two days after cyclosporin withdrawal. We concluded that a) the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cyclosporin; Hyperalgesia; Edema; Adjuvant arthritis.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100016

Abnormalities of glucose metabolism in spontaneously hypertensive rats
Provedor de dados: 56
Autores: Gouveia,L.M.F.B.; Kettelhut,I.C.; Foss,M.C.
Abnormalities in glucose metabolism and insulin action are frequently detected in patients with essential hypertension. Spontaneously hypertensive rats (SHR) have been used as an experimental model to understand this pathological condition. The objective of the present study was to assess glucose metabolism and insulin action in SHR and Wistar rats under fed and fasting conditions. Peripheral glucose utilization was estimated by kinetic studies with [6-³H]-glucose and gluconeogenetic activity was measured during continuous [14C]-bicarbonate infusion. Plasma glucose levels were higher in the SHR group. Plasma insulin levels in the fed state were higher in the SHR group (99.8 ± 6.5 µM) than in the control group (70.4 ± 3.6 µM). Muscle glycogen content was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peripheral glucose utilization; Gluconeogenesis; Insulin resistance; Spontaneously hypertensive rats.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100015

Gender-related differences in the effects of nitric oxide donors on neuroleptic-induced catalepsy in mice
Provedor de dados: 56
Autores: Pires,J.G.P.; Costa,P.G.; Saraiva,F.P.; Bonikovski,V.; Futuro Neto,H.A.
It has been suggested that nigrostriatal dopaminergic transmission is modulated by nitric oxide (NO). Since there is evidence that gonadal hormones can affect extrapyramidal motor behavior in mammals, we investigated the effects of isosorbide dinitrate (ISD), linsidomine (SIN-1) and S-nitroso-N-acetylpenicillamine (SNAP), three pharmacologically different NO donors, on neuroleptic-induced catalepsy in 60- to 80-day-old male and female albino mice. Catalepsy was induced with haloperidol (1 mg/kg, ip) and measured at 30-min intervals by means of a bar test. Drugs (or appropriate vehicle) were injected ip 30 min before haloperidol, with each animal being used only once. ISD (5, 20 and 50 mg/kg) caused a dose-dependent inhibition of catalepsy in male mice...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuroleptic-induced catalepsy; Nitric oxide donors; Gender differences; Linsidomine; SNAP; Isosorbide dinitrate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200012

Density, proportion, and dendritic coverage of retinal ganglion cells of the common marmoset (Callithrix jacchus jacchus)
Provedor de dados: 56
Autores: Gomes,F.L.; Silveira,L.C.L.; Saito,C.A.; Yamada,E.S.
We performed a quantitative analysis of M and P cell mosaics of the common-marmoset retina. Ganglion cells were labeled retrogradely from optic nerve deposits of Biocytin. The labeling was visualized using horseradish peroxidase (HRP) histochemistry and 3-3'diaminobenzidine as chromogen. M and P cells were morphologically similar to those found in Old- and New-World primates. Measurements were performed on well-stained cells from 4 retinas of different animals. We analyzed separate mosaics for inner and outer M and P cells at increasing distances from the fovea (2.5-9 mm of eccentricity) to estimate cell density, proportion, and dendritic coverage. M cell density decreased towards the retinal periphery in all quadrants. M cell density was higher in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnocellular pathway; Parvocellular pathway; Visual system; Parallel processing; New World primates.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600014

Collagen arrangement in hepatic granuloma in mice infected with Schistosoma mansoni: dependence on fiber radiation centers
Provedor de dados: 56
Autores: Lenzi,H.L.; Kimmel,E.; Schechtman,H.; Pelajo-Machado,M.; Vale,B.S.; Panasco,M.S.; Lenzi,J.A.
The collagen structure of isolated and in situ liver granuloma from Swiss Webster mice infected with Schistosoma mansoni was sequentially and three-dimensionally analyzed during different times of infection (early acute, acute, transitional acute-chronic, and chronic phases) by laser scanning confocal microscopy and electron scanning variable vacuum microscopy. The initial granuloma structure is characterized by vascular collagen residues and by anchorage points (or fiber radiation centers), from where collagenous fibers are angularly shed and self-assembled. During the exudative-productive stage, the self-assembly of these fibers minimizes energy and mass through continuous tension and focal compression. The curvature or angles between collagen fibers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Granuloma; Schistosoma mansoni; Collagen; Extracellular matrix; Confocal microscopy.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500018

Ischemic preconditioning vs adenosine vs prostaglandin E1 for protection against liver ischemia/reperfusion injury
Provedor de dados: 56
Autores: Radojkovic,M.; Stojanovic,M.; Stanojevic,G.; Radojkovic,D.; Gligorijevic,J.; Ilic,I.; Stojanovic,N.
Ischemia/reperfusion injury is still a major cause of morbidity and mortality during liver surgery and transplantation. A variety of surgical and pharmacological therapeutic strategies have been investigated to minimize the effects of ischemia/reperfusion. The aim of our study was to analyze and compare preventive influences of ischemic preconditioning, adenosine and prostaglandin E1 in the experimental model of hepatic ischemia/reperfusion injury. Adult chinchilla rabbits were divided into four groups: 10 rabbits subjected to liver ischemic preconditioning (3-min period of inflow occlusion followed by a 5-min period of reperfusion) followed by 45 min of Pringle maneuver; 10 rabbits subjected to pre-treatment with intraportal injection of adenosine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver ischemia/reperfusion injury; Ischemic preconditioning; Adenosine; Prostaglandin E1; Protection.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800611

Early responses of the STAT3 pathway to platinum drugs are associated with cisplatin resistance in epithelial ovarian cancer
Provedor de dados: 56
Autores: Sheng,W.J.; Jiang,H.; Wu,D.L.; Zheng,J.H.
Cisplatin resistance remains one of the major obstacles when treating epithelial ovarian cancer. Because oxaliplatin and nedaplatin are effective against cisplatin-resistant ovarian cancer in clinical trials and signal transducer and activator of transcription 3 (STAT3) is associated with cisplatin resistance, we investigated whether overcoming cisplatin resistance by oxaliplatin and nedaplatin was associated with the STAT3 pathway in ovarian cancer. Alamar blue, clonogenic, and wound healing assays, and Western blot analysis were used to compare the effects of platinum drugs in SKOV-3 cells. At an equitoxic dose, oxaliplatin and nedaplatin exhibited similar inhibitory effects on colony-forming ability and greater inhibition on cell motility than cisplatin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epithelial ovarian cancer; Cisplatin; Oxaliplatin; Nedaplatin; STAT3.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800650

Involvement of dopamine receptors in diethylpropion-induced conditioning place preference
Provedor de dados: 56
Autores: Planeta,C.S.; DeLucia,R.
Diethylpropion (DEP) is an amphetamine-like agent used as an anorectic drug. Abuse of DEP has been reported and some restrictions of its use have been recently imposed. The conditioning place preference (CPP) paradigm was used to evaluate the reinforcing properties of DEP in adult male Wistar rats. After initial preferences were determined, animals weighing 250-300 g (N = 7 per group) were conditioned with DEP (10, 15 or 20 mg/kg). Only the dose of 15 mg/kg produced a significant place preference (358 ± 39 vs 565 ± 48 s). Pretreatment with the D1 antagonist SCH 23390 (0.05 mg/kg, sc) 10 min before DEP (15 mg/kg, ip) blocked DEP-induced CPP (418 ± 37 vs 389 ± 31 s) while haloperidol (0.5 mg/kg, ip), a D2 antagonist, 15 min before DEP was ineffective in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diethylpropion; Conditioning place preference; Reinforcing effect; Dopamine receptors.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400014

Decreased platelet responsiveness to clopidogrel correlates with CYP2C19 and PON1 polymorphisms in atherosclerotic patients
Provedor de dados: 56
Autores: Marchini,J.F.M.; Pinto,M.R.; Novaes,G.C.; Badran,A.V.; Pavão,R.B.; Figueiredo,G.L.; Lago,I.M.; Lima-Filho,M.O.; Lemos,D.C.; Tonani,M.; Antloga,C.M.; Oliveira,L.; Lorenzi,J.C.; Marin-Neto,J.A.
Clopidogrel and aspirin are the most commonly used medications worldwide for dual antiplatelet therapy after percutaneous coronary intervention. However, clopidogrel hyporesponsiveness related to gene polymorphisms is a concern. Populations with higher degrees of genetic admixture may have increased prevalence of clopidogrel hyporesponsiveness. To assess this, we genotyped CYP2C19, ABCB1, and PON1 in 187 patients who underwent percutaneous coronary intervention. Race was self-defined by patients. We also performed light transmission aggregometry with adenosine diphosphate (ADP) and arachidonic acid during dual antiplatelet therapy. We found a significant difference for presence of the CYP2C19*2 polymorphism between white and non-white patients. Although 7%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Platelet function tests; Single nucleotide polymorphism; Percutaneous coronary intervention; Aspirin; Clopidogrel.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100702

Quality of life on hemodialysis and inflammation: a descriptive analysis
Provedor de dados: 56
Autores: Bacci,M.R.; Adami,F.; Figueiredo,F.W.S.; Alves,B.C.A.; da Veiga,G.L.; Fonseca,F.L.A.
Chronic kidney disease (CKD) is highly prevalent worldwide. Patients with CKD on hemodialysis are more likely to present behavioral changes and worse quality of life as a result of their routine and complications. They also have higher levels of cytokines. The aim of this study is to assess the relationship between the inflammatory profile and quality of life measured by KDOQL-SF36 in hemodialysis outpatients. Patients older than 21 years of age and on routine hemodialysis for at least 6 months with treatment on a regular weekly basis were included and their anthropometric parameters and serum inflammatory markers were evaluated. Thirty patients consented to participate. Homocysteine (Hcy) levels were correlated with worse glomerular filtration rate (GFR;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quality of life; Hemodialysis; Inflammation; Homocysteine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600608

High expression of human carboxypeptidase M in Pichia pastoris: Purification and partial characterization
Provedor de dados: 56
Autores: Craveiro,R.B.; Ramalho,J.D.; Chagas,J.R.; Wang,P.H.M.; Casarini,D.E.; Pesquero,J.L.; Araújo,R.C.; Pesquero,J.B.
Carboxypeptidase M (CPM) is an extracellular glycosylphosphatidyl-inositol-anchored membrane glycoprotein, which removes the C-terminal basic residues, lysine and arginine, from peptides and proteins at neutral pH. CPM plays an important role in the control of peptide hormones and growth factor activity on the cell surface. The present study was carried out to clone and express human CPM in the yeast Pichia pastoris in order to evaluate the importance of this enzyme in physiological and pathological processes. The cDNA for the enzyme was amplified from total placental RNA by RT-PCR and cloned in the vector pPIC9, which uses the methanol oxidase promoter and drives the expression of high levels of heterologous proteins in P. pastoris. The cpm gene, after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kinins; Human carboxypeptidase M; Recombinant protein; Pichia pastoris.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200007

Glutamatergic transmission in the nucleus tractus solitarii: from server to peripherals in the cardiovascular information superhighway
Provedor de dados: 56
Afferent nerves carrying signals from mechanoreceptors in the aortic arch and carotid sinus terminate predominantly in the nucleus tractus solitarii (NTS). Signal transduction and neurotransmission in the NTS are critical for central cardiovascular reflex control, but little was known about either until the late 1970's. None of the numerous neuroactive chemicals found in the NTS had met strict criteria as a neurotransmitter in the baroreflex arc until data suggested that the excitatory amino acid L-glutamate (GLU) might be released from baroreceptor afferent terminals in the NTS. In anesthetized animals microinjection into the NTS of GLU, which can be demonstrated in terminals in the NTS, produces cardiovascular responses like those seen with activation of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glutamate; Nucleus tractus solitarii; Baroreceptor reflex; Cardiovascular; Vagus; Mechanoreceptors.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100001

A conjugate of an anti-midkine single-chain variable fragment to doxorubicin inhibits tumor growth
Provedor de dados: 56
Autores: Zhao,Shuli; Zhao,Guangfeng; Xie,Hao; Huang,Yahong; Hou,Yayi.
Doxorubicin (DOX) was conjugated to a single-chain variable fragment (scFv) against human midkine (MK), and the conjugate (scFv-DOX) was used to target the chemotherapeutic agent to a mouse solid tumor model in which the tumor cells expressed high levels of human MK. The His-tagged recombinant scFv was expressed in bacteria, purified by metal affinity chromatography, and then conjugated to DOX using oxidative dextran (Dex) as a linker. The molecular formula of this immunoconjugate was scFv(Dex)1.3(DOX)20. In vitro apoptosis assays showed that the scFv-DOX conjugate was more cytotoxic against MK-transfected human adenocarcinoma cells (BGC823-MK) than untransfected cells (55.3 ± 2.4 vs 22.4 ± 3.8%) for three independent experiments. Nude mice bearing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single-chain variable fragments; Midkine; Tumor targeting; Doxorubicin.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300008

Contractile profile of esophageal and gastric fundus strips in experimental doxorubicin-induced esophageal atresia
Provedor de dados: 56
Autores: Capeto,F.A.; Lima,F.J.B.; Okoba,W.; Ramos,F.L.; Messias,T.F.A.; Rigonatto,G.A.; Sbragia,L.; Magalhães,P.J.C.; Melo-Filho,A.A.
Esophageal atresia (EA) is characterized by esophageal and gastric motility changes secondary to developmental and postsurgical damage. This study evaluated the in vitro contractile profile of the distal esophagus and gastric fundus in an experimental model of EA induced by doxorubicin (DOXO). Wistar pregnant rats received DOXO 2.2 mg/kg on the 8th and 9th gestational days. On day 21.5, fetuses were collected, sacrificed, and divided into groups: control, DOXO without EA (DOXO-EA), and DOXO with EA (DOXO+EA). Strips from the distal esophagus and gastric fundus were mounted on a wire myograph and isolated organ-bath system, respectively, and subjected to increasing concentrations of carbamylcholine chloride (carbachol, CCh). The isolated esophagus was also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esophageal atresia; Doxorubicin; Experimental; Esophageal contractility.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500458

Cat ownership is associated with increased asthma prevalence and dog ownership with decreased spirometry values
Provedor de dados: 56
Autores: Simoneti,C.S.; Ferraz,E.; Menezes,M.B.; Icuma,T.R.; Vianna,E.O.
The association between pet ownership and the development of allergic and respiratory diseases has been the aim of several studies, however, the effects of exposure in adults remain uncertain. The aims of the present study were to investigate the prevalence of asthma and lung function status among dog and cat owners. This cross-sectional study was performed at two universities with students and workers who were allocated into 3 groups according to pet ownership in the previous year: cat owners, dog owners, and no pets (control group). Subjects underwent spirometry, bronchial challenge test with mannitol, skin prick tests, and questionnaires about animal exposures and respiratory symptoms. Control group comprised 125 subjects; cat owner group, 51 subjects;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Respiratory tract diseases; Animals; Bronchial hyperreactivity; Lung function; Pets.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200601

Pharmacokinetic/pharmacodynamic relationships of FTY720 in kidney transplant recipients
Provedor de dados: 56
Autores: Park,S.I.; Felipe,C.R.; Machado,P.G.; Garcia,R.; Skerjanec,A.; Schmouder,R.; Tedesco-Silva Jr.,H.; Medina-Pestana,J.O.
FTY720 is a new and effective immunosuppressive agent, which produces peripheral blood lymphopenia through a lymphocyte homing effect. We investigated the relationship between the dose of FTY720 or blood concentration (pharmacokinetics, PK) and peripheral lymphopenia (pharmacodynamics, PD) in 23 kidney transplant recipients randomized to receive FTY720 (0.25-2.5 mg/day) or mofetil mycophenolate (2 mg/day) in combination with cyclosporine and steroids. FTY720 dose, blood concentrations and lymphocyte counts were determined weekly before and 4 to 12 weeks after transplantation. The effect of PD was calculated as the absolute lymphocyte count or its reductions. PK/PD modeling was used to find the best-fit model. Mean FTY720 concentrations were 0.36 ± 0.05...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FTY720; Lymphopenia; Pharmacokinetics; Pharmacodynamics; Immunosuppression; Renal transplants.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500005

Experimental chronic entrapment of the sciatic nerve in adult hamsters: an ultrastructural and morphometric study
Provedor de dados: 56
Autores: Prinz,R.A.D.; Nakamura-Pereira,M.; De-Ary-Pires,B.; Fernandes,D.S.; Fabião-Gomes,B.D.S.V.; Bunn,P.S.; Martinez,A.M.B.; Pires-Neto,M.A.; Ary-Pires,R.
Entrapment neuropathy is a group of clinical disorders involving compression of a peripheral nerve and interference with nerve function mostly through traction injury. We have investigated the chronic compression of peripheral nerves as an experimental procedure for detecting changes in ultrastructural nerve morphology. Adult hamsters (Mesocricetus auratus, N = 30) were anesthetized with a 25% pentobarbital solution and received a cuff around the right sciatic nerve. Left sciatic nerves were not operated (control group). Animals survived for varying times (up to 15 weeks), after which they were sacrificed and both sciatic nerves were immediately fixed with a paraformaldehyde solution. Experimental nerves were divided into segments based upon their distance...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nerve entrapment; Ultrastructure; Morphometry; Hamster; Renaut body.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900015

The ERBB2 gene polymorphisms rs2643194, rs2934971, and rs1058808 are associated with increased risk of gastric cancer
Provedor de dados: 56
Autores: Vázquez-Ibarra,K.C.; Bustos-Carpinteyro,A.R.; García-Ruvalcaba,A.; Magaãa-Torres,M.T.; Gutiérrez-Aguilar,R.; Marín-Contreras,M.E.; Santiago-Luna,E.; Sánchez-López,J.Y.
Gastric cancer (GC) is the third most lethal type of cancer worldwide. Single nucleotide polymorphisms (SNPs) in regulatory sites or coding regions can modify the expression of genes involved in gastric carcinogenesis, as ERBB2, which encodes for the tyrosine-kinase receptor HER-2. The aim of this work was to analyze the association of the polymorphisms: rs2643194, rs2517951, rs2643195, rs2934971, and rs1058808 with GC, as they have not yet been analyzed in GC patients, as well as to report their frequency in the general Mexican population (GMP). We studied genomic DNA from subjects with GC (n=74), gastric inflammatory diseases (GID, n=76 control subjects), and GMP (n=102). Genotypes were obtained by means of real-time PCR and DNA-sequencing. The risks for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ERBB2 gene; Gastric cancer; HER2; Polymorphisms.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500611

Repercussions of adjuvant-induced arthritis on body composition, soleus muscle, and heart muscle of rats
Provedor de dados: 56
Autores: Pita,L.M.; Spadella,M.A.; Montenote,M.C.; Oliveira,P.B.; Chies,A.B.
This study investigated the repercussions of adjuvant-induced arthritis (AIA) on body composition and the structural organization of the soleus and cardiac muscles, including their vascularization, at different times of disease manifestation. Male rats were submitted to AIA induction by intradermal administration of 100 μL of Mycobacterium tuberculosis (50 mg/mL), in the right hind paw. Animals submitted to AIA were studied 4 (AIA4), 15 (AIA15), and 40 (AIA40) days after AIA induction as well as a control group of animals not submitted to AIA. Unlike the control animals, AIA animals did not gain body mass throughout the evolution of the disease. AIA reduced food consumption, but only on the 40th day after induction. In the soleus muscle, AIA reduced the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental arthritis; Cachexia; Musculoskeletal; Microcirculation; Cardiovascular; Body composition.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300607

Long-term loss of color vision after exposure to mercury vapor
Provedor de dados: 56
Autores: Feitosa-Santana,C.; Costa,M.F.; Lago,M.; Ventura,D.F.
We evaluated the color vision of 24 subjects (41.6 ± 6.5 years; 6 females) who worked in fluorescent lamp industries. They had been occupationally exposed to mercury vapor (10.6 ± 5.2 years) and had been away from the source of exposure for 6.4 ± 4.04 years. Mean urinary concentration of mercury was 40.6 ± 36.4 µg/g creatinine during or up to 1 year after exposure and 2.71 ± 1.19 µg/g creatinine at the time of color vision testing or up to 1 year thereafter. All patients were diagnosed with chronic mercury intoxication, characterized by clinical symptoms and neuropsychological alterations. A control group (N = 36, 48.6 ± 11.9 years, 10 females, 1.5 ± 0.47 µg mercury/g creatinine) was subjected to the same tests. Inclusion criteria for both groups were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Color vision; Chromatic discrimination; Color discrimination; Psychophysics; Mercury intoxication; Occupational exposure.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300017

Retraction of the paper: "Niemann-Pick type C1 protein influences the delivery of cholesterol to the SREBP:SCAP complex"
Provedor de dados: 56
Autores: Guo,Yong-Jun; Li,Wei-Hua; Wu,Rong; Xie,Qiang; Zhang,Zu-Huang; Cui,Lian-Qun.
Tipo: Info:eu-repo/semantics/article
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500014

In vitro cultivation of canine multipotent mesenchymal stromal cells on collagen membranes treated with hyaluronic acid for cell therapy and tissue regeneration
Provedor de dados: 56
Autores: Wodewotzky,T.I.; Lima-Neto,J.F.; Pereira-Júnior,O.C.M.; Sudano,M.J.; Lima,S.A.F.; Bersano,P.R.O.; Yoshioka,S.A.; Landim-Alvarenga,F.C.
Support structures for dermal regeneration are composed of biodegradable and bioresorbable polymers, animal skin or tendons, or are bacteria products. The use of such materials is controversial due to their low efficiency. An important area within tissue engineering is the application of multipotent mesenchymal stromal cells (MSCs) to reparative surgery. The combined use of biodegradable membranes with stem cell therapy may lead to promising results for patients undergoing unsuccessful conventional treatments. Thus, the aim of this study was to test the efficacy of using membranes composed of anionic collagen with or without the addition of hyaluronic acid (HA) as a substrate for adhesion and in vitro differentiation of bone marrow-derived canine MSCs. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collagen scaffolds; Biomaterials; Hyaluronic acid; Mesenchymal stromal cells; Tissue engineering; Cell culture.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200008

Expression of the chemokine receptor CXCR4 on lymphocytes of leprosy patients
Provedor de dados: 56
Autores: Mendonça,V.A.; Alvim de Melo,G.E.B.; Araújo,M.G.; Borges,V.O.; Costa,R.D.; Martins-Filho,O.A.; Teixeira- Carvalho,A.; Sathler-Avelar,R.; Teixeira,M.M.; Teixeira,A.L.
Leprosy is caused by Mycobacterium leprae, which induces chronic granulomatous infection of the skin and peripheral nerves. The disease ranges from the tuberculoid to the lepromatous forms, depending on the cellular immune response of the host. Chemokines are thought to be involved in the immunopathogenesis of leprosy, but few studies have investigated the expression of chemokine receptors on leukocytes of leprosy patients. In the present study, we evaluated 21 leprosy patients (M/F: 16/5) with a new diagnosis from the Dermatology Outpatient Clinic of the University Hospital, Federal University of Minas Gerais. The control group was composed of 20 healthy members (M/F: 15/5) of the community recruited by means of announcements. The expression of CCR2,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leprosy; Chemokine receptors; CXCR4; Leukocytes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200009

Different levels of brain-derived neurotrophic factor and cortisol in healthy heavy smokers
Provedor de dados: 56
Autores: Neves,C.D.C.; Lacerda,A.C.R.; Lima,L.P.; Lage,V.K.S.; Balthazar,C.H.; Leite,H.R.; Mendonça,V.A.
Studies suggest that brain-derived neurotrophic factor (BDNF) and the hypothalamic-pituitary-adrenal (HPA) axis modulate dopaminergic activity in response to nicotine and that the concentrations of BDNF and cortisol seem to be dependent on the amount and duration of smoking. Therefore, we investigated BDNF and cortisol levels in smokers ranked by daily cigarette consumption. Twenty-seven adult males (13 non-smokers and 14 smokers) participated in the study. The smokers were divided in two groups: light (n=7) and heavy smokers (n=7). Anthropometric parameters and age were paired between the groups, and plasma BDNF and salivary cortisol levels were measured. Saliva samples were collected on awakening, 30 min after awakening, at 10:00 and 12:00 am, 5:00 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BDNF; Nicotine consumption; HPA axis; Addiction; Reward system.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200608

A moderate decrease in temperature inhibits the calcium signaling mechanism(s) of the regulatory volume decrease in chick embryo cardiomyocytes
Provedor de dados: 56
Autores: Souza,M.M.; Boyle,R.T.
Chick cardiomyocytes, when submitted to hyposmotic swelling, exhibit a partial regulatory volume decrease (RVD). A Ca2+ influx by stretch-activated channels signals a taurine efflux and the RVD at 37°C. We evaluated the cell's performance at room temperature. Cardiomyocytes isolated and cultured from 11-day-old chick embryos were submitted to a hyposmotic solution (180 mOsm/kg H2O) at 37°C and at room temperature (26°C). Under these conditions we measured the changes in cell volume as well as the intracellular free Ca2+ (using fura-2). During hyposmotic swelling, cells at 37°C displayed a peak relative volume of 1.61 ± 0.03 and recovery to 1.22 ± 0.04 (N = 14), while cells at 26°C presented a peak swell relative volume of 1.74 ± 0.06 and did not recover...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cell volume; RVD; Room temperature; High free calcium.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100018

Electrical field stimulation improves bone mineral density in ovariectomized rats
Provedor de dados: 56
Autores: Lirani-Galvão,A.P.R.; Bergamaschi,C.T.; Silva,O.L.; Lazaretti-Castro,M.
Osteoporosis and its consequent fractures are a great social and medical problem mainly occurring in post-menopausal women. Effective forms of prevention and treatment of osteoporosis associated with lower costs and the least side effects are needed. Electrical fields are able to stimulate osteogenesis in fractures, but little is known about their action on osteoporotic tissue. The aim of the present study was to determine by bone densitometry the effects of electrical stimulation on ovariectomized female Wistar rats. Thirty rats (220 ± 10 g) were divided into three groups: sham surgery (SHAM), bilateral ovariectomy (OVX) and bilateral ovariectomy + electrical stimulation (OVX + ES). The OVX + ES group was submitted to a 20-min session of a low-intensity...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rats; Osteoporosis; Ovariectomy; Electrical stimulation; Bone mineral density; Osteogenesis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100014

Mechanisms involved in the vasorelaxant effects produced by the acute application of amfepramone in vitro to rat aortic rings
Provedor de dados: 56
Autores: López-Canales,J.S.; Lozano-Cuenca,J.; Muãoz-Islas,E.; Aguilar-Carrasco,J.C.; López-Canales,O.A.; López-Mayorga,R.M.; Castillo-Henkel,E.F.; Valencia-Hernández,I.; Castillo-Henkel,C.
Amfepramone (diethylpropion) is an appetite-suppressant drug used for the treatment of overweight and obesity. It has been suggested that the systemic and central activity of amfepramone produces cardiovascular effects such as transient ischemic attacks and primary pulmonary hypertension. However, it is not known whether amfepramone produces immediate vascular effects when applied in vitro to rat aortic rings and, if so, what mechanisms may be involved. We analyzed the effect of amfepramone on phenylephrine-precontracted rat aortic rings with or without endothelium and the influence of inhibitors or blockers on this effect. Amfepramone produced a concentration-dependent vasorelaxation in phenylephrine-precontracted rat aortic rings that was not affected by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular pharmacology; Amfepramone; Obesity; Rat aorta; Nitric oxide; Potassium channels.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600537

Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae)
Provedor de dados: 56
Autores: Silva-Pinhati,A.C.O.; Bacci Jr.,M.; Hinkle,G.; Sogin,M.L.; Pagnocca,F.C.; Martins,V.G.; Bueno,O.C.; Hebling,M.J.A.
Leaf-cutting ants of the genera Atta and Acromyrmex (tribe Attini) are symbiotic with basidiomycete fungi of the genus Leucoagaricus (tribe Leucocoprineae), which they cultivate on vegetable matter inside their nests. We determined the variation of the 28S, 18S, and 5.8S ribosomal DNA (rDNA) gene loci and the rapidly evolving internal transcribed spacers 1 and 2 (ITS1 and ITS2) of 15 sympatric and allopatric fungi associated with colonies of 11 species of leafcutter ants living up to 2,600 km apart in Brazil. We found that the fungal rDNA and ITS sequences from different species of ants were identical (or nearly identical) to each other, whereas 10 GenBank Leucoagaricus species showed higher ITS variation. Our findings suggest that Atta and Acromyrmex...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atta; Acromyrmex; Internal transcribed spacer; Leaf-cutting ants; Leucoagaricus; Leucoagaricus gongylophorus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000004

Reduced hippocampal GABAergic function in Wistar audiogenic rats
Provedor de dados: 56
Autores: Drumond,L.E.; Kushmerick,C.; Guidine,P.A.M.; Doretto,M.C.; Moraes,M.F.D.; Massensini,A.R.
Epilepsy is a neurological disorder associated with excitatory and inhibitory imbalance within the underlying neural network. This study evaluated inhibitory γ-amino-butyric acid (GABA)ergic modulation in the CA1 region of the hippocampus of male Wistar rats and Wistar audiogenic rats (aged 90 ± 3 days), a strain of inbred animals susceptible to audiogenic seizures. Field excitatory postsynaptic potentials and population spike complexes in response to Schaffer collateral fiber stimulation were recorded in hippocampal slices before and during application of picrotoxin (50 µM, 60 min), a GABA A antagonist, and the size of the population spike was quantified by measuring its amplitude and slope. In control audiogenic-resistant Wistar rats (N = 9), picrotoxin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epilepsy; Extracellular recording; GABA; Hippocampus; Wistar audiogenic rats; Picrotoxin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000011

CorrectionBraz J Med Biol Res 2011; 44(8): 793-800Candidate gene linkage analysis indicates genetic heterogeneity in Marfan syndrome
Provedor de dados: 56
Autores: Teixeira,L.V.S.; Lezirovitz,K.; Pereira,L.V.; Perez,A.B.A.
Tipo: Info:eu-repo/semantics/other
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000014

Resistance of multicellular aggregates to pharmorubicin observed in human hepatocarcinoma cells
Provedor de dados: 56
Autores: Jianmin,Z.; Hongfang,W.; Meifu,F.
The objective of the present study was to investigate the multicellular resistance of human hepatocarcinoma cells BEL-7402 to pharmorubicin. Cells (1 x 10(4)) and 200 microcarrier Cytodex-3 beads were seeded onto a 24-well plate and cultured in RPMI 1640 medium. After the formation of multicellular aggregates, morphology and cell viability were analyzed by scanning electron microscopy, transmission electron microscopy and flow cytometry, respectively. The IC50 was determined by flow cytometry and MTT assay after the cells cultured in aggregates and monolayers were treated with pharmorubicin. The culture products exhibited structural characteristics somewhat similar to those of trabecular hepatocarcinoma in vivo. Among the microcarriers, cells were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; Spheroids; Drug resistance; Microcarrier; Epirubicin.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200015

Erythrocyte glucose-6-phosphate dehydrogenase from Brazilian opossum Didelphis marsupialis
Provedor de dados: 56
Autores: Barretto,O.C. de O.; Oshiro,M.; Oliveira,R.A.G.; Fedullo,J.D.L.; Nonoyama,K.
In a comparative study of erythrocyte metabolism of vertebrates, the specific activity of glucose-6-phosphate dehydrogenase (G6PD) of the Brazilian opossum Didelphis marsupialis in a hemolysate was shown to be high, 207 ± 38 IU g-1 Hb-1 min-1 at 37ºC, compared to the human erythrocyte activity of 12 ± 2 IU g-1 Hb-1 min-1 at 37ºC. The apparent high specific activity of the mixture led us to investigate the physicochemical properties of the opossum enzyme. We report that reduced glutathione (GSH) in the erythrocytes was only 50% higher than in human erythrocytes, a value lower than expected from the high G6PD activity since GSH is maintained in a reduced state by G6PD activity. The molecular mass, determined by G-200 Sephadex column chromatography at pH 8.0,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Erythrocyte glucose-6-phosphate dehydrogenase; Didelphis marsupialis; Erythrocyte glutathione.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500007

Analysis of HLA-A antigens and C282Y and H63D mutations of the HFE gene in Brazilian patients with hemochromatosis
Provedor de dados: 56
Autores: Bittencourt,P.L.; Palácios,S.A.; Couto,C.A.; Cançado,E.L.R.; Carrilho,F.J.; Laudanna,A.A.; Kalil,J.; Gayotto,L.C.C.; Goldberg,A.C.
The hemochromatosis gene, HFE, is located on chromosome 6 in close proximity to the HLA-A locus. Most Caucasian patients with hereditary hemochromatosis (HH) are homozygous for HLA-A3 and for the C282Y mutation of the HFE gene, while a minority are compound heterozygotes for C282Y and H63D. The prevalence of these mutations in non-Caucasian patients with HH is lower than expected. The objective of the present study was to evaluate the frequencies of HLA-A antigens and the C282Y and H63D mutations of the HFE gene in Brazilian patients with HH and to compare clinical and laboratory profiles of C282Y-positive and -negative patients with HH. The frequencies of HLA-A and C282Y and H63D mutations were determined by PCR-based methods in 15 male patients (median...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemochromatosis; HFE mutations; Iron overload; HLA-A3 mutation; C282Y mutation; H63D mutation; Chromosome 6.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300007

Cytokine expression patterns and mesenchymal stem cell karyotypes from the bone marrow microenvironment of patients with myelodysplastic syndromes
Provedor de dados: 56
Autores: Xiong,H.; Yang,X.Y.; Han,J.; Wang,Q.; Zou,Z.L.
The purpose of this study was to explore cytokine expression patterns and cytogenetic abnormalities of mesenchymal stem cells (MSCs) from the bone marrow microenvironment of Chinese patients with myelodysplastic syndromes (MDS). Bone marrow samples were obtained from 30 cases of MDS (MDS group) and 30 healthy donors (control group). The expression pattern of cytokines was detected by customized protein array. The karyotypes of MSCs were analyzed using fluorescence in situ hybridization. Compared with the control group, leukemia inhibitory factor, stem cell factor (SCF), stromal cell-derived factor (SDF-1), bone morphogenetic protein 4, hematopoietic stem cell (HSC) stimulating factor, and transforming growth factor-β in the MDS group were significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow microenvironment; Cytokine; Mesenchymal stem cell; Chromosome; Pathogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300207

Propranolol inhibits myocardial infarction-induced brown adipose tissue D2 activation and maintains a low thyroid hormone state in rats
Provedor de dados: 56
Autores: Seara,F.A.C.; Araujo,I.G.; Império,G.E.; Marassi,M.P.; Silva,A.C.M.; Mecawi,A.S.; Reis,L.C.; Olivares,E.L.
Considering the recognized role of thyroid hormones on the cardiovascular system during health and disease, we hypothesized that type 2 deiodinase (D2) activity, the main activation pathway of thyroxine (T4)-to-triiodothyronine (T3), could be an important site to modulate thyroid hormone status, which would then constitute a possible target for β-adrenergic blocking agents in a myocardial infarction (MI) model induced by left coronary occlusion in rats. Despite a sustained and dramatic fall in serum T4 concentrations (60–70%), the serum T3 concentration fell only transiently in the first week post-infarction (53%) and returned to control levels at 8 and 12 weeks after surgery compared to the Sham group (P&lt;0.05). Brown adipose tissue (BAT) D2 activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial infarction; Thyroid hormone; Deiodinase; Beta-blocker; Rats.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000609

Health in early adulthood: the contribution of the 1978/79 Ribeirão Preto birth cohort
Provedor de dados: 56
Autores: Barbieri,M.A.; Bettiol,H.; Silva,A.A.M.; Cardoso,V.C.; Simões,V.M.F.; Gutierrez,M.R.P.; Castro,J.A.S.; Vianna,E.S.O.; Foss,M.C.; Dos Santos,J.E.; Queiroz,R.G.P.
The increase in non-communicable chronic diseases of adults is due to demographic changes and changes in the risk factors related to physical activity, smoking habits and nutrition. We describe the methodology for the evaluation of persons at 23/25 years of age of a cohort of individuals born in Ribeirão Preto in 1978/79. We present their socioeconomic characteristics and the profile of some risk factors for chronic diseases. A total of 2063 participants were evaluated by means of blood collection, standardized questionnaires, anthropometric and blood pressure measurements, and methacholine bronchoprovocation tests. The sexes were compared by the chi-square test, with alpha = 0.05. Obesity was similar among men and women (12.8 and 11.1%); overweight was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-communicable chronic diseases of adults; Metabolic syndrome; Obesity; Arterial hypertension Asthma; Dyslipidemia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800007

The study of genetic polymorphisms related to serotonin in Alzheimer's disease: a new perspective in a heterogenic disorder
Provedor de dados: 56
Autores: Oliveira,J.R.M.; Zatz,M.
Genetic and environmental factors have been implicated in the development of Alzheimer's disease (AD), the most common form of dementia in the elderly. Mutations in 3 genes mapped on chromosomes 21, 14 and 1 are related to the rare early onset forms of AD while the &lt;FONT FACE="Symbol"&gt;e&lt;/font&gt;4 allele of the apolipoprotein E (APOE) gene (on chromosome 19) is the major susceptibility locus for the most common late onset AD (LOAD). Serotonin (5-hydroxytryptamine or 5-HT) is a key neurotransmitter implicated in the control of mood, sleep, appetite and a variety of traits and behaviors. Recently, a polymorphism in the transcriptional control region upstream of the 5-HT transporter (5-HTT) gene has been studied in several psychiatric diseases and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alzheimer's disease; Allelic association; Serotonin polymorphisms.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400014

Is arterial stiffness in HIV-infected individuals associated with HIV-related factors?
Provedor de dados: 56
Autores: Monteiro,P.; Miranda-Filho,D.B.; Bandeira,F.; Lacerda,H.R.; Chaves,H.; Albuquerque,M.F.P.M.; Montarroyos,U.R.; Ximenes,R.A.A.
We investigated the association between pulse wave velocity (PWV) and HIV infection, antiretroviral treatment-related characteristics, viral load, immune status, and metabolic changes in a cross-sectional study nested in a cohort of HIV/AIDS patients who have been followed for metabolic and cardiovascular changes since 2007. The study included patients recruited from the cohort (N = 261) and a comparison group (N = 82) of uninfected individuals, all enrolled from April to November 2009. Aortic stiffness was estimated using the carotid-femoral PWV (Complior-Artech, Paris, France). The groups were similar with respect to age, metabolic syndrome, diabetes mellitus, Framingham score, and use of antihypertensive and hypolipidemic medications. Hypertension was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; AIDS; Antiretroviral therapy; Arterial stiffness; Pulse wave velocity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900005

The photodynamic and non-photodynamic actions of porphyrins
Provedor de dados: 56
Autores: Afonso,S.G.; Enríquez de Salamanca,R.; Batlle,A.M. del C.
Porphyrias are a family of inherited diseases, each associated with a partial defect in one of the enzymes of the heme biosynthetic pathway. In six of the eight porphyrias described, the main clinical manifestation is skin photosensitivity brought about by the action of light on porphyrins, which are deposited in the upper epidermal layer of the skin. Porphyrins absorb light energy intensively in the UV region, and to a lesser extent in the long visible bands, resulting in transitions to excited electronic states. The excited porphyrin may react directly with biological structures (type I reactions) or with molecular oxygen, generating excited singlet oxygen (type II reactions). Besides this well-known photodynamic action of porphyrins, a novel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic; Porphyrias; Porphyrins; Protein alterations.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300002

Early effects of estrogen on the rat ventral prostate
Provedor de dados: 56
Autores: García-Flórez,M.; Oliveira,C.A.; Carvalho,H.F.
Complex interactions between androgen and estrogen (E2) regulate prostatic development and physiology. We analyzed the early effects of a high single dose of E2 (25 mg/kg body weight) and castration (separately or combined) on the adult 90-day-old male Wistar rat ventral prostate. Androgen levels, prostate weight, and the variation in the relative and absolute volume of tissue compartments and apoptotic indices were determined for 7 days. Castration and exogenous E2 markedly reduced ventral prostate weight (about 50% of the control), with a significant reduction in the epithelial compartment and increased stroma. The final volume of the epithelium was identical at day 7 for all treatments (58.5% of the control). However, E2 had an immediate effect, causing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Estrogen; Prostate; Stereology; Tissue kinetics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400002

Factors associated with infant and adolescent mortality
Provedor de dados: 56
Autores: Oliveira,Z.A.R.; Bettiol,H.; Gutierrez,M.R.P.; Silva,A.A.M.; Barbieri,M.A.
Few studies have described factors associated with infant and adolescent mortality since birth. We report here mortality during a 20-year period in a birth cohort from Ribeirão Preto in order to identify birth variables that influenced mortality among infants and children between 10 and 19 years of age, the main causes of death, and the influence of social inequality at birth on death. Mothers were interviewed shortly after delivery. Social, biological and demographic information was collected, and mortality up to 19 years of age was investigated in registry systems. Of the 6748 liveborn singletons born in the municipality from 1978 to 1979, 343 died before or when 19 years of age were completed. Most of the cohort mortality (74.9%) occurred during the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infant mortality; Adolescence; Causes of death; External causes; Socioeconomic factors; Social inequality; 20-year Brazilian cohort study.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900012

Effect of penehyclidine hydrochloride on β-arrestin-1 expression in lipopolysaccharide-induced human pulmonary microvascular endothelial cells
Provedor de dados: 56
Autores: Zhan,J.; Xiao,F.; Zhang,Z.Z.; Wang,Y.P.; Chen,K.; Wang,Y.L.
β-arrestins are expressed proteins that were first described, and are well-known, as negative regulators of G protein-coupled receptor signaling. Penehyclidine hydrochloride (PHC) is a new anti-cholinergic drug that can inhibit biomembrane lipid peroxidation, and decrease cytokines and oxyradicals. However, to date, no reports on the effects of PHC on β-arrestin-1 in cells have been published. The aim of this study was to investigate the effect of PHC on β-arrestin-1 expression in lipopolysaccharide (LPS)-induced human pulmonary microvascular endothelial cells (HPMEC). Cultured HPMEC were pretreated with PHC, followed by LPS treatment. Muscarinic receptor mRNAs were assayed by real-time quantitative PCR. Cell viability was assayed by the methyl thiazolyl...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penehyclidine hydrochloride; Human pulmonary microvascular endothelial cells; Β-arrestin-1.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201040

Human group C rotavirus in children with diarrhea in the Federal District, Brazil
Provedor de dados: 56
Autores: Teixeira,J.M.S.; Camara,G.N.N.L.; Pimentel,P.F.V.; Ferreira,M.N.R.; Ferreira,M.S.R.; Alfieri,A.A.; Gentsch,J.R.; Leite,J.P.G.
Group C rotaviruses are fastidious in their in vitro cell culture requirements. Recent serosurveys indicate that antibody to group C rotavirus is present in 3-45% of the human population in certain geographic locations, suggesting that rotavirus group C infection is more prevalent than previously believed and that the low rate of detection of these agents is probably due to the lack of sensitive diagnostic assays. From March to December 1994, 406 fecal specimens were collected from children under five years of age who were outpatients at the emergency services of nine public hospitals in Brasília, Federal District, Brazil. In addition to the samples from children, one public outpatient unit requested virological investigation of a stool sample from an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Group C rotavirus; Diarrhea; HIV; RT-PCR; Probe hybridization.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100005

Correlation of ultrasonography synovitis with disease activity and clinical response to etanercept treatment in juvenile idiopathic arthritis patients
Provedor de dados: 56
Autores: Zhou,Li; Gu,Xiaojie.
This study aimed to investigate the correlation of ultrasonography (US) of synovitis with disease activity and clinical response to etanercept (ETN) in juvenile idiopathic arthritis (JIA) patients. Eighty-two JIA patients who underwent ETN treatment for 24 weeks were consecutively enrolled. US evaluations of 28 joints (shoulder, elbow, wrist, metacarpophalangeal, and proximal interphalangeal of hands and knee) at baseline were performed using grey-scale US and power doppler (PD) US, and US synovitis was defined as grey-scale abnormalities or PD abnormalities. Clinical response was assessed according to the ACRpedi 50 response criteria. In total, 2296 joints were scanned and 608 (26.5%) joints presented US synovitis, which was numerically higher than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ultrasonography Synovitis; Disease activity Clinical response Etanercept.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200603

Leptin is not associated independently with hypertension in Japanese-Brazilian women
Provedor de dados: 56
Autores: Almeida-Pititto,B.; Gimeno,S.G.A.; Freire,R.D.; Ribeiro-Filho,F.F.; Ferreira,S.R.G.
We evaluated the relationship of leptin with hypertension adjusted for body mass index (BMI) and/or waist circumference in a population of Japanese-Brazilian women aged  &gt; or = 30 years with centrally distributed adiposity. After excluding diabetic subjects, the study subjects - who participated in a population-based study on the prevalence of metabolic syndrome - showed prevalence rates of obesity (BMI  &gt; or = 25 kg/m²) and central adiposity (waist  &gt; or = 80 cm) of 32.0 and 37.8%, respectively. The hypertensive group (N = 162) was older, had higher BMI (24.9 ± 4.2 vs 23.3 ± 3.4 kg/m², P &lt; 0.001), waist circumference (81.1 ± 10.1 vs 76.3 ± 8.2 cm, P &lt; 0.001) and insulin levels (8.0 ± 6.2 vs 7.1 ± 4.9 µU/mL, P &lt; 0.05) than the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Body fat distribution; Insulin; Leptin; Centrally obese; Metabolic syndrome.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100012

FAMLF is a target of miR-181b in Burkitt lymphoma
Provedor de dados: 56
Autores: Li,J.G.; Ding,Y.; Huang,Y.M.; Chen,W.L.; Pan,L.L.; Li,Y.; Chen,X.L.; Chen,Y.; Wang,S.Y.; Wu,X.N.
Burkitt lymphoma (BL) is a highly malignant non-Hodgkin's lymphoma that is closely related to the abnormal expression of genes. Familial acute myelogenous leukemia related factor (FAMLF; GenBank accession No. EF413001.1) is a novel gene that was cloned by our research group, and miR-181b is located in the intron of the FAMLF gene. To verify the role of miR-181b and FAMLF in BL, RNAhybrid software was used to predict target site of miR-181b on FAMLF and real-time quantitative PCR (RQ-PCR) was used to detect expression of miR-181b and FAMLF in BL patients, Raji cells and unaffected individuals. miR-181b was then transfected into Raji and CA46 cell lines and FAMLF expression was examined by RQ-PCR and western blotting. Further, Raji cells viability and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Burkitt lymphoma; FAMLF; MiR-181b; Gene expression; RQ-PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600701

In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics
Provedor de dados: 56
Autores: Sun,Dao-Cai; Li,De-Hua; Ji,Hui-Cang; Rao,Guo-Zhou; Liang,Li-Hua; Ma,Ai-Jie; Xie,Chao; Zou,Gui-Ke; Song,Ying-Liang.
In order to understand the mechanisms of poor osseointegration following dental implants in type 2 diabetics, it is important to study the biological properties of alveolar bone osteoblasts isolated from these patients. We collected alveolar bone chips under aseptic conditions and cultured them in vitro using the tissue explants adherent method. The biological properties of these cells were characterized using the following methods: alkaline phosphatase (ALP) chemical staining for cell viability, Alizarin red staining for osteogenic characteristics, MTT test for cell proliferation, enzyme dynamics for ALP contents, radio-immunoassay for bone gla protein (BGP) concentration, and ELISA for the concentration of type I collagen (COL-I) in the supernatant....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alveolar bone; Cell culture; Implant; Osteoblast; Type 2 diabetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600005

Simultaneous allergen inactivation and detoxification of castor bean cake by treatment with calcium compounds
Provedor de dados: 56
Autores: Fernandes,K.V.; Deus-de-Oliveira,N.; Godoy,M.G.; Guimarães,Z.A.S.; Nascimento,V.V.; Melo,E.J.T. de; Freire,D.M.G.; Dansa-Petretski,M.; Machado,O.L.T.
Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor oil has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, as a component of plastic products, as a fungicide or in the synthesis of biodiesel fuels. After oil extraction, a castor cake with a large amount of protein is obtained. However, this by-product cannot be used as animal feed due to the presence of toxic (ricin) and allergenic (2S albumin) proteins. Here, we propose two processes for detoxification and allergen inactivation of the castor cake. In addition, we establish a biological test to detect ricin and validate these detoxification processes. In this test, Vero cells were treated with ricin, and cell death...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ricinus communis; 2S albumin; Ricin; Biodiesel fuel; Solid-state fermentation; Vero cell.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100002

Comparison of serum hormone levels of captive and free-living maned wolves Chrysocyon brachyurus
Provedor de dados: 56
Autores: Maia,O.B.; Jácomo,A.T.A.; Bringel,B.A.; Kashivakura,C.K.; Oliveira,C.A.; Teodoro,L.O.F.; Silveira,L.; Teixeira da Costa,M.E.L.; Malta,M.C.C.; Furtado,M.M.; Torres,N.M.; Mattos,P.S.R.; Viau,.P; Lima,T.F.G.; Morato,R.G.
Serum hormone levels were compared between captive and free-living maned wolves and seasonal variations of sex hormones were studied. Blood samples were collected from 16 male and 26 female adult animals from Brazilian zoos, and from 30 male and 24 female free-living adults to determine serum progesterone and testosterone by radioimmunoassay. Serum testosterone concentrations varied (P &lt; 0.05) across seasons for 16 captive males, being higher in autumn (2184.7 ± 355.1 pg/mL) than in summer (1080.7 ± 205.4 pg/mL), winter (1270.1 ± 276.6 pg/mL) and spring (963.9 ± 248.1 pg/mL), although they did not differ between summer, winter and spring. Testosterone concentration of 30 free-living males differed (P &lt; 0.05) between autumn (824.1 ± 512.2 pg/mL),...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Maned wolf Chrysocyon brachyurus; Progesterone; Testosterone; Radioimmunoassay; Reproduction.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200015

A study on the short-term effect of cafeteria diet and pioglitazone on insulin resistance and serum levels of adiponectin and ghrelin
Provedor de dados: 56
Autores: Colombo,G.; Bazzo,M.L.; Nogueira,C.L.; Colombo,M.D.H.P.; Schiavon,L.L.; d’Acampora,A.J.
The interaction between ghrelin and adiponectin is still controversial. We investigated the effect of cafeteria diet and pioglitazone on body weight, insulin resistance, and adiponectin/ghrelin levels in an experimental study on male Wistar rats. The animals were divided into four groups of 6 rats each, and received balanced chow with saline (CHOW-O) or pioglitazone (CHOW-P), or a cafeteria diet with saline (CAFE-O) or pioglitazone (CAFE-P). The chow/cafeteria diets were administered for 35 days, and saline/pioglitazone (10 mg·kg body weight-1·day-1) was added in the last 14 days prior to euthanasia. CAFE-O animals had a higher mean final weight (372.5 ± 21.01 g) than CHOW-O (317.66 ± 25.11 g, P = 0.017) and CHOW-P (322.66 ± 28.42 g, P = 0.035) animals....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diet; Insulin resistance; Adiponectin; Ghrelin; Thiazolidinediones.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000008

Interleukin-15 as a potential regulator of the innate immune response
Provedor de dados: 56
Autores: Carson,W.; Caligiuri,M.A.
Interleukin-15 (IL-15) is a newly-discovered cytokine that is produced by activated monocytes early in the course of the innate immune response. IL-15 is able to bind to components of the interleukin-2 receptor (IL-2R) despite the fact that it has no sequence homology with IL-2. IL-15 stimulates human natural killer cell proliferation, cytotoxicity, and cytokine production and can substitute for IL-2 under most conditions. In vitro studies indicate that monocyte-derived IL-15 may be an important determinant of IFN-gamma production by NK cells. In addition, IL-15 is able to promote the survival of natural killer cells under serum-free conditions. The IL-15 receptor is a heterotrimeric complex which is composed of the IL-2Rß and &lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interleukin-15; Interleukin-12; Gamma interferon; Natural killer cell; Innate immunity.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100001

Mechanisms involved in calcium oxalate endocytosis by Madin-Darby canine kidney cells
Provedor de dados: 56
Autores: Campos,A.H.; Schor,N.
Calcium oxalate (CaOx) crystals adhere to and are internalized by tubular renal cells and it seems that this interaction is related (positively or negatively) to the appearance of urinary calculi. The present study analyzes a series of mechanisms possibly involved in CaOx uptake by Madin-Darby canine kidney (MDCK) cells. CaOx crystals were added to MDCK cell cultures and endocytosis was evaluated by polarized light microscopy. This process was inhibited by an increase in intracellular calcium by means of ionomycin (100 nM; N = 6; 43.9% inhibition; P&amp;lt;0.001) or thapsigargin (1 µM; N = 6; 33.3% inhibition; P&amp;lt;0.005) administration, and via blockade of cytoskeleton assembly by the addition of colchicine (10 µM; N = 8; 46.1% inhibition;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium oxalate; DCK cells; Mechanisms of endocytosis; Renal stone.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100015

Radioautographology: the proposal of a new concept
Provedor de dados: 56
Autores: Nagata,T.
A new concept termed "radioautographology" is advocated. This term was synthesized from "radioautography" and "ology", expressing a new science derived from radioautography. The concept of radioautographology (RAGology) is that of a science whose objective is to localize radioactive substances in the biological structure of objects and to analyze and study the significance of these substances in the biological structure. On the other hand, the old term radioautography (RAG) is the technique used to demonstrate the pattern of localization of various radiolabeled compounds in specimens. The specimens used in biology and medicine are cells and tissues. They are fixed, sectioned and placed in contact with the radioautographic emulsions, which are exposed and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Light microscopy; Electron microscopy; Radioautography; Technique; Application.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800001

Proteolytic release and partial characterization of human sperm-surface glycopeptides
Provedor de dados: 56
Sperm-surface glycopeptides were obtained from intact sperm membranes after proteolytic release by different enzymatic treatments such as autoproteolysis, trypsin, papain and pronase. Glycopeptides were isolated, their properties and composition were examined, and their monosaccharide and amino acid constituents were characterized. The monosaccharides identified were fucose, mannose, galactose, N-acetylglucosamine, and N-acetylgalactosamine, which form part of more than one type of oligosaccharide units. Autoproteolytic treatment mainly provided O-glycosidic type oligosaccharides, while a mixture of O- and N-glycosidic oligosaccharides was obtained in variable proportions when treated with trypsin, papain or pronase. The highest degree of peptide cleavage...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Carbohydrate; Glycoconjugate; Proteolysis; Human sperm; Peptide.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300013

Characterization of bacteriophages infecting clinical isolates of Pseudomonas aeruginosa stored in a culture collection
Provedor de dados: 56
Autores: Zanetti,C.C.S.; Mingrone,R.C.C.; Kisielius,J.J.; Ueda-Ito,M.; Pignatari,A.C.C.
Some clinical isolates of Pseudomonas aeruginosa stored in our culture collection did not grow or grew poorly and showed lysis on the culture plates when removed from the collection and inoculated on MacConkey agar. One hypothesis was that bacteriophages had infected and killed those clinical isolates. To check the best storage conditions to maintain viable P. aeruginosa for a longer time, clinical isolates were stored at various temperatures and were grown monthly. We investigated the presence of phage in 10 clinical isolates of P. aeruginosa stored in our culture collection. Four strains of P. aeruginosa were infected by phages that were characterized by electron microscopy and isolated to assess their ability to infect. The best condition to maintain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteriophages; Pseudomonas aeruginosa.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800689

Inhibition of rat microsomal lipid peroxidation by the oral administration of D002
Provedor de dados: 56
Autores: Menéndez,R.; Amor,A.M.; González,R.M.; Jiménez,S.; Más,R.
The effect of D002, a defined mixture of higher primary alcohols purified from bee wax, on in vivo and in vitro lipid peroxidation was studied. The extent of lipid peroxidation was measured on the basis of the levels of thiobarbituric acid reactive substances (TBARS). When D002 (5-100 mg/kg body weight) was administered orally to rats for two weeks, a partial inhibition of the in vitro enzymatic and non-enzymatic lipid peroxidation was observed in liver and brain microsomes. Maximal protection (46%) occurred at a dose of 25 mg/kg. D002 behaved differently depending on both the presence of NADPH and the integrity of liver microsomes, which suggests that under conditions where microsomal metabolism was favored the protective effect of D002 was increased....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: D002; Higher aliphatic alcohols; Microsomes; Lipids; Peroxidation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100012

Transplantation and survival of mouse inner ear progenitor/stem cells in the organ of Corti after cochleostomy of hearing-impaired guinea pigs: preliminary results
Provedor de dados: 56
Autores: Barboza Jr.,L.C.M.; Lezirovitz,K.; Zanatta,D.B.; Strauss,B.E.; Mingroni-Netto,R.C.; Oiticica,J.; Haddad,L.A.; Bento,R.F.
In mammals, damage to sensory receptor cells (hair cells) of the inner ear results in permanent sensorineural hearing loss. Here, we investigated whether postnatal mouse inner ear progenitor/stem cells (mIESCs) are viable after transplantation into the basal turns of neomycin-injured guinea pig cochleas. We also examined the effects of mIESC transplantation on auditory functions. Eight adult female Cavia porcellus guinea pigs (250-350g) were deafened by intratympanic neomycin delivery. After 7 days, the animals were randomly divided in two groups. The study group (n=4) received transplantation of LacZ-positive mIESCs in culture medium into the scala tympani. The control group (n=4) received culture medium only. At 2 weeks after transplantation, functional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cochlea; Hearing loss; Stem cells; Cell transplantation; Ototoxicity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400603

Simultaneous changes in the function and expression of beta 1 integrins during the growth arrest of poorly differentiated colorectal cells (LISP-1)
Provedor de dados: 56
Autores: Roela,R.A.; Brentani,M.M.; Katayama,M.L.H.; Reis,M.; Federico,M.H.H.
Cells usually lose adhesion and increase proliferation and migration during malignant transformation. Here, we studied how proliferation can affect the other two characteristics, which ultimately lead to invasion and metastasis. We determined the expression of ß1 integrins, as well as adhesion and migration towards laminin-1, fibronectin, collagens type I and type IV presented by LISP-1 colorectal cancer cells exposed to 2.5% dimethyl sulfoxide (DMSO), an agent capable of decreasing proliferation in this poorly differentiated colorectal cell line. Untreated cells (control), as shown by flow cytometry and monoclonal antibodies, expressed alpha2 (63.8 ± 11.3% positive cells), alpha3 (93.3 ± 7.0%), alpha5 (50.4 ± 12.0%) and alpha6 (34.1 ± 4.9%) integrins but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell adhesion molecules; Cell migration; Integrin; Cell cycle; DMSO.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800016

Effect of 17ß-estradiol or alendronate on the bone densitometry, bone histomorphometry and bone metabolism of ovariectomized rats
Provedor de dados: 56
Autores: da Paz,L.H.B.C.; de Falco,V.; Teng,N.C.; dos Reis,L.M.; Pereira,R.M.R.; Jorgetti,V.
The objective of the present study was to evaluate the effect of 17ß-estradiol or alendronate in preventing bone loss in 3-month-old ovariectomized Wistar rats. One group underwent sham ovariectomy (control, N = 10), and the remaining three underwent double ovariectomy. One ovariectomized group did not receive any treatment (OVX, N = 12). A second received subcutaneous 17ß-estradiol at a dose of 30 µg/kg for 6 weeks (OVX-E, N = 11) and a third, subcutaneous alendronate at a dose of 0.1 mg/kg for 6 weeks (OVX-A, N = 8). Histomorphometry, densitometry, osteocalcin and deoxypyridinoline measurements were applied to all groups. After 6 weeks there was a significant decrease in bone mineral density (BMD) at the trabecular site (distal femur) in OVX rats. Both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat; 17ß-estradiol; Alendronate; Densitometry; Histomorphometry.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800007

Financial support of graduate programs in Brazil: quo vadis?
Provedor de dados: 56
Autores: Helene,A.F.; Xavier,G.F.
Graduate programs provide the highest level of formal education and thus are crucial for the development of any country. However, official Brazilian data clearly show a dramatic decrease in the number and values of scholarships available to graduate programs in Brazil over the last few years, despite the importance and growth of such programs. Between 1995 and 2004, investment by the Coordenadoria de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES, subordinate to the Ministry of Education and Culture) in funding scholarships, corrected for inflation in the period, actually decreased by 51%. In addition, during the period between 1994 and 2004, there was a loss of about 60% in the purchasing power of the graduate scholarships provided by CAPES and the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Graduate programs; Scholarships; Financial support; PhD program; Doctoral degree; Master's degree.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700001

Enhanced anti-tumor efficacy of 5-aminolevulinic acid-gold nanoparticles-mediated photodynamic therapy in cutaneous squamous cell carcinoma cells
Provedor de dados: 56
Autores: Chi,Yu-fei; Qin,Jing-jing; Li,Zhi; Ge,Qin; Zeng,Wei-hui.
The objective of this study was to investigate whether the conjugation of gold nanoparticles (GNPs) to 5-aminolevulinic acid (5-ALA) could enhance the anti-tumor efficiency of photodynamic therapy (PDT) in epidermoid carcinoma cells. The mRNA and protein expression levels were determined by quantitative real-time PCR and western blot, respectively. Cell viability, apoptosis, invasion, and migration were determined by MTT assay, flow cytometry, transwell invasion assay, and migration assay, respectively. Singlet oxygen generation was detected by the singlet oxygen sensor green reagent assay. Our results showed that PDT with 5-ALA and GNPs-conjugated 5-ALA (5-ALA-GNPs) significantly suppressed cell viability, increased cell apoptosis and singlet oxygen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic therapy; 5-ALA-GNPs; Cell viability; Cell apoptosis; Cell invasion and migration; Cutaneous squamous cell carcinoma.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500608

Effect of sildenafil citrate on the cardiovascular system
Provedor de dados: 56
Autores: Shinlapawittayatorn,K.; Chattipakorn,S.; Chattipakorn,N.
Sildenafil citrate is a drug commonly used to manage erectile dysfunction. It is designated chemically as 1-[[3-(6,7-dihydro-1-methyl-7-oxo-3-propyl-1H -pyrazolo[4,3-d]pyrimidin-5-yl)-4 ethoxyphenyl] sulfonyl]-4-methylpiperazine citrate (C22H30N6O4 S). It is a highly selective inhibitor of cyclic guanine monophosphate-specific phosphodiesterase type 5. In late March through mid-November 1998, the US Food and Drug Administration (FDA) published a report on 130 confirmed deaths among men (mean age, 64 years) who received prescriptions for sildenafil citrate, a period during which &gt;6 million outpatient prescriptions (representing about 50 million tablets) were dispensed. The US FDA recently reported that significant cardiovascular events, including sudden...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sildenafil citrate; Erectile dysfunction; Sudden cardiac death; Cardiovascular disease; Phosphodiesterase inhibitor; Cardioprotection.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900003

Serum antigliadin antibody levels as a screening criterion before jejunal biopsy indication for celiac disease in a developing country
Provedor de dados: 56
Autores: Bahia,M.; Rabello,A.; Brasileiro Filho,G.; Penna,F.J.
The objective of the present study was to determine the efficacy of detection of antigliadin immunoglobulins G and A (IgG and IgA) for the diagnosis of celiac disease in a developing country, since other enteropathies might alter the levels of these antibodies. Three groups were studied: 22 patients with celiac disease (mean age: 30.6 months), 61 patients with other enteropathies (mean age: 43.3 months), and 46 patients without enteropathies (mean age: 96.9 months). Antigliadin IgG and IgA ELISA showed sensitivity of 90.9 and 95.5%, respectively. With the hypothetical values of prevalence ranging from 1:500 to 1:2000 liveborns, the positive predictive value varied from 8.5 to 2.3% for IgG and from 4.8 to 1.1% for IgA. Considering the patients without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Celiac disease; Enteropathies; Antigliadin antibodies.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100007

Epidemiological and functional implications of molecular variants of human papillomavirus
Provedor de dados: 56
Autores: Sichero,L.; Villa,L.L.
Human papillomavirus genomes are classified into molecular variants when they present more than 98% of similarity to the prototype sequence within the L1 gene. Comparative nucleotide sequence analyses of these viruses have elucidated some features of their phylogenetic relationship. In addition, human papillomavirus intratype variability has also been used as an important tool in epidemiological studies of viral transmission, persistence and progression to clinically relevant cervical lesions. Until the present, little has been published concerning the functional significance of molecular variants. It has been shown that nucleotide variability within the long control region leads to differences in the binding affinity of some cellular transcriptional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; DNA sequencing; Oncogenic potential; Cervical cancer; Immunity; Epidemiology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600002

Alternative pathways for angiotensin II generation in the cardiovascular system
Provedor de dados: 56
Autores: Becari,C.; Oliveira,E.B.; Salgado,M.C.O.
The classical renin-angiotensin system (RAS) consists of enzymes and peptides that regulate blood pressure and electrolyte and fluid homeostasis. Angiotensin II (Ang II) is one of the most important and extensively studied components of the RAS. The beneficial effects of angiotensin converting enzyme (ACE) inhibitors in the treatment of hypertension and heart failure, among other diseases, are well known. However, it has been reported that patients chronically treated with effective doses of these inhibitors do not show suppression of Ang II formation, suggesting the involvement of pathways alternative to ACE in the generation of Ang II. Moreover, the finding that the concentration of Ang II is preserved in the kidney, heart and lungs of mice with an ACE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin-angiotensin system; Angiotensin II; Rat elastase-2; Alternative pathway; Angiotensin-converting enzyme.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900013

Nucleotide excision repair pathway assessment in DNA exposed to low-intensity red and infrared lasers
Provedor de dados: 56
Autores: Fonseca,A.S.; Campos,V.M.A.; Magalhães,L.A.G.; Paoli,F.
Low-intensity lasers are used for prevention and management of oral mucositis induced by anticancer therapy, but the effectiveness of treatment depends on the genetic characteristics of affected cells. This study evaluated the survival and induction of filamentation of Escherichia coli cells deficient in the nucleotide excision repair pathway, and the action of T4endonuclease V on plasmid DNA exposed to low-intensity red and near-infrared laser light. Cultures of wild-type (strain AB1157) E. coli and strain AB1886 (deficient in uvrA protein) were exposed to red (660 nm) and infrared (808 nm) lasers at various fluences, powers and emission modes to study bacterial survival and filamentation. Also, plasmid DNA was exposed to laser light to study DNA lesions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Enzyme; Escherichia coli; Filamentation; Laser.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000011

Evaluation of stunned and infarcted canine myocardium by real time myocardial contrast echocardiography
Provedor de dados: 56
Autores: Dourado,P.M.M.; Tsutsui,J.M.; Mathias Jr.,W.; Andrade,J.L.; da Luz,P.L.; Chagas,A.C.P.
Differentiation between stunned and infarcted myocardium in the setting of acute ischemia is challenging. Real time myocardial contrast echocardiography allows the simultaneous assessment of myocardial perfusion and function. In the present study we evaluated infarcted and stunned myocardium in an experimental model using real time myocardial contrast echocardiography. Sixteen dogs underwent 180 min of coronary occlusion followed by reperfusion (infarct model) and seven other dogs were submitted to 20 min of coronary occlusion followed by reperfusion (stunned model). Wall motion abnormality and perfusional myocardial defect areas were measured by planimetry. Risk and infarct areas were determined by tissue staining. In the infarct model, the wall motion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial perfusion; Contrast echocardiography; Myocardial stunning; Myocardial infarction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100009

Role of chemokines in promoting instability of coronary atherosclerotic plaques and the underlying molecular mechanism
Provedor de dados: 56
Autores: Zhong,Z.X.; Li,B.; Li,C.R.; Zhang,Q.F.; Liu,Z.D.; Zhang,P.F.; Gu,X.F.; Luo,H.; Li,M.J.; Luo,H.S.; Ye,G.H.; Wen,F.L.
Our aim was to investigate the role of chemokines in promoting instability of coronary atherosclerotic plaques and the underlying molecular mechanism. Coronary angiography and intravascular ultrasound (IVUS) were performed in 60 stable angina pectoris (SAP) patients and 60 unstable angina pectoris (UAP) patients. The chemotactic activity of monocytes in the 2 groups of patients was examined in Transwell chambers. High-sensitivity C-reactive protein (hs-CRP), monocyte chemoattractant protein-1 (MCP-1), regulated on activation in normal T-cell expressed and secreted (RANTES), and fractalkine in serum were examined with ELISA kits, and expression of MCP-1, RANTES, and fractalkine mRNA was examined with real-time PCR. In the SAP group, 92 plaques were detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Chemokine; Plaque; MCP-1; RANTES.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200161

Primary cultures of mouse small intestinal epithelial cells using the dissociating enzyme type I collagenase and hyaluronidase
Provedor de dados: 56
Autores: Ren,H.J.; Zhang,C.L.; Liu,R.D.; Li,N.; Li,X.G.; Xue,H.K.; Guo,Y.; Wang,Z.Q.; Cui,J.; Ming,L.
The epithelium is a highly dynamic system, which plays a crucial role in the homeostasis of the intestinal tract. However, studies on the physiological and pathophysiological functions of intestinal epithelial cells (IECs) have been hampered due to lack of normal epithelial cell models. In the present study, we established a reproducible method for primary culture of mouse IECs, which were isolated from the viable small intestinal crypts of murine fetuses (on embryonic day 19), using type I collagenase and hyaluronidase in a short span of time (≤20 min). With this method, continuously growing mouse IECs, which can be subcultured over a number of passages, were obtained. The obtained cell lines formed a tight cobblestone-like arrangement, displayed long and...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Intestinal epithelial cells; Crypt; Isolation; Primary culture; Collagenase I; Hyaluronidase.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500604

MicroRNA-143 inhibits cell growth by targeting ERK5 and MAP3K7 in breast cancer
Provedor de dados: 56
Autores: Zhou,L.L.; Dong,J.L.; Huang,G.; Sun,Z.L.; Wu,J.
This study aimed to investigate the function and mechanism of microRNA-143 (miR-143) in the occurrence and development of breast cancer (BC). A total of 30 BC tissues, 30 corresponding noncancerous tissues, and 10 normal control (NC) breast tissues were obtained to detect the levels of miR-143, extracellular signal-regulated kinase 5 (ERK5) and mitogen-activated protein 3 kinase 7 (MAP3K7) using RT-qPCR, western blotting or immunohistochemistry. The correlation of miR-143 with ERK5 or MAP3K7 was evaluated using Pearson correlation analysis. MCF-7 cells were transiently transfected with miR-143 mimic, miR-143 inhibitor, miR-143 mimic/inhibitor + si-ERK5, si-MAP3K7 or si-cyclin D1. Then, cell growth was evaluated by MTT assay and the expressions of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular signal-regulated kinase 5; Mitogen-activated protein 3 kinase 7; MiRNA-143; Breast cancer; Cyclin D1.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800608

Exploration of gene functions for esophageal squamous cell carcinoma using network-based guilt by association principle
Provedor de dados: 56
Autores: Wu,Wei; Huang,Bo; Yan,Yan; Zhong,Zhi-Qiang.
Gene networks have been broadly used to predict gene functions based on guilt by association (GBA) principle. Thus, in order to better understand the molecular mechanisms of esophageal squamous cell carcinoma (ESCC), our study was designed to use a network-based GBA method to identify the optimal gene functions for ESCC. To identify genomic bio-signatures for ESCC, microarray data of GSE20347 were first downloaded from a public functional genomics data repository of Gene Expression Omnibus database. Then, differentially expressed genes (DEGs) between ESCC patients and controls were identified using the LIMMA method. Afterwards, construction of differential co-expression network (DCN) was performed relying on DEGs, followed by gene ontology (GO) enrichment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esophageal squamous cell carcinoma; Gene oncology; Guilt by association; Differentially expressed genes; Area under the curve.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600601

Immunophenotype of normal and leukemic bone marrow B-precursors in a Brazilian population. A comparative analysis by quantitative fluorescence cytometry
Provedor de dados: 56
Autores: Rego,E.M.; Garcia,A.B.; Carneiro,J.J.; Falcão,R.P.
The distinction between normal and leukemic bone marrow (BM) B-precursors is essential for the diagnosis and treatment monitoring of acute lymphoblastic leukemia (ALL). In order to evaluate the potential use of quantitative fluorescence cytometry (QFC) for this distinction, we studied 21 normal individuals and 40 patients with CD10+ ALL. We characterized the age-related changes of the CD10, CD19, TdT, CD34 and CD79a densities in normal and leukemic BM. Compared to normal adults, the B-precursors from normal children expressed significantly lower values of CD34-specific antibody binding capacity (SABC) (median value of 86.6 vs 160.2 arbitrary units (a.u.) in children and adults, respectively). No significant age-related difference was observed in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute lymphoblastic leukemia; Lymphocytes; Quantitative fluorescence cytometry; Flow cytometry; Age-related changes.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200005

Effect of sildenafil (Viagra®) on the genital reflexes of paradoxical sleep-deprived male rats
Provedor de dados: 56
Autores: Andersen,M.L.; Bignotto,M.; Tufik,S.
Since there is evidence that paradoxical sleep deprivation (PSD) elicits penile erection (PE) and ejaculation (EJ), and that the erectile response of rats is mediated by nitric oxide, the present study sought to extend the latter finding by assessing the effects of sildenafil on the genital reflexes of male Wistar rats subjected to PSD. We also determined the influence of sildenafil on hormone concentrations. In the first experiment, sildenafil at doses ranging from 0.08 to 0.32 mg/kg was administered intraperitoneally to rats that had been deprived of sleep for 4 days and to home cage controls (N = 8-10/group). The frequency of PE and EJ was measured for 60 min. PSD alone induced PE in 50% of the animals; however, a single injection of sildenafil did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paradoxical sleep deprivation; Sildenafil; Genital reflexes; Dopamine; Testosterone; Progesterone.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100008

Chronotype ontogeny related to gender
Provedor de dados: 56
Autores: Duarte,L.L.; Menna-Barreto,L.; Miguel,M.A.L.; Louzada,F.; Araújo,J.; Alam,M.; Areas,R.; Pedrazzoli,M.
Chronotype is an established concept designed to identify distinct phase relationships between the expression of circadian rhythms and external synchronizers in humans. Although it has been widely accepted that chronotype is subjected to ontogenetic modulation, there is no consensus on the interaction between age and gender. This study aimed to determine the relationship between age- and gender-related changes in the morningness-eveningness character in a large sample of people. A total of 14,650 volunteers were asked to complete the Brazilian version of the Horne and Östberg chronotype questionnaire. The data demonstrated that, on average, women were more morning-oriented than men until the age of 30 and there were no significant differences between men...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronotype; Gender; Ontogeny; Circadian rhythms; Sleep timing.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400316

Extralymphatic disease due to bancroftian filariasis
Provedor de dados: 56
Autores: Dreyer,G.; Dreyer,P.; Piessens,W.F.
Infection with Wuchereria bancrofti, Brugia malayi, or B. timori not only affects the structure and function of lymphatic vessels but is also associated with extralymphatic pathology and disease. Because it is now possible to detect living adult worms by ultrasonography, much emphasis is placed on lymphatic pathology. However, the finding of renal damage in asymptomatic microfilaremic carriers has led to increased recognition of the importance of extralymphatic clinical manifestation in bancroftian filariasis. The authors present a number of clinical syndromes that may be manifestations of extralymphatic filarial disease and discuss possible mechanisms that cause these conditions. The main purpose of this paper is to raise the awareness of students and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wuchereria bancrofti; Microfilaria; Extralymphatic disease; Tropical pulmonary eosinophilia.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200003

The role of reelin in the development and evolution of the cerebral cortex
Provedor de dados: 56
Autores: Tissir,F.; Lambert de Rouvroit,C.; Goffinet,A.M.
Reelin is an extracellular matrix protein that is defective in reeler mutant mice and plays a key role in the organization of architectonic patterns, particularly in the cerebral cortex. In mammals, a "reelin signal" is activated when reelin, secreted by Cajal-Retzius neurons, binds to receptors of the lipoprotein receptor family on the surface of cortical plate cells, and triggers Dab1 phosphorylation. As reelin is a key component of cortical development in mammals, comparative embryological studies of reelin expression were carried out during cortical development in non-mammalian amniotes (turtles, squamates, birds and crocodiles) in order to assess the putative role of reelin during cortical evolution. The data show that reelin is present in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reeler; Dab1; VLDLR; ApoER2.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001200007

Use of molecular epidemiology to monitor the nosocomial dissemination of methicillin-resistant Staphylococcus aureus in a University Hospital from 1991 to 2001
Provedor de dados: 56
Autores: Beretta,A.L.R.Z.; Trabasso,P.; Stucchi,R.B.; Moretti,M.L.
Methicillin-resistant Staphylococcus aureus (MRSA) has been the cause of major outbreaks and epidemics among hospitalized patients, with high mortality and morbidity rates. We studied the genomic diversity of MRSA strains isolated from patients with nosocomial infection in a University Hospital from 1991 to 2001. The study consisted of two periods: period I, from 1991 to 1993 and period II from 1995 to 2001. DNA was typed by pulsed-field gel electrophoresis and the similarity among the MRSA strains was determined by cluster analysis. During period I, 73 strains presented five distinctive DNA profiles: A, B, C, D, and E. Profile A was the most frequent DNA pattern and was identified in 55 (75.3%) strains; three closely related and four possibly related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methicillin-resistant Staphylococcus aureus; Molecular epidemiology; Hospital infection; Pulsed-field gel electrophoresis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900009

Pim-1 kinase inhibits the activation of reporter gene expression in Elk-1 and c-Fos reporting systems but not the endogenous gene expression: an artifact of the reporter gene assay by transient co-transfection
Provedor de dados: 56
Autores: Yan,B.; Wang,H.; Kon,T.; Li,C.-Y.
We have studied the molecular mechanism and signal transduction of pim-1, an oncogene encoding a serine-threonine kinase. This is a true oncogene which prolongs survival and inhibits apoptosis of hematopoietic cells. In order to determine whether the effects of Pim-1 occur by regulation of the mitogen-activated protein kinase pathway, we used a transcriptional reporter assay by transient co-transfection as a screening method. In this study, we found that Pim-1 inhibited the Elk-1 and NFkappaB transcriptional activities induced by activation of the mitogen-activated protein kinase cascade in reporter gene assays. However, Western blots showed that the induction of Elk-1-regulated expression of endogenous c-Fos was not affected by Pim-1. The phosphorylation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pim-1 kinase; Signal transduction; Mitogen-activated protein kinase pathway; Elk-1; Reporter assay.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200002

The effect of an aerobic training program on the electrical remodeling of the heart: high-frequency components of the signal-averaged electrocardiogram are predictors of the maximal aerobic power
Provedor de dados: 56
Autores: Marocolo,M.; Nadal,J.; Benchimol-Barbosa,P.R.
Increased heart rate variability (HRV) and high-frequency content of the terminal region of the ventricular activation of signal-averaged ECG (SAECG) have been reported in athletes. The present study investigates HRV and SAECG parameters as predictors of maximal aerobic power (VO2max) in athletes. HRV, SAECG and VO2max were determined in 18 high-performance long-distance (25 ± 6 years; 17 males) runners 24 h after a training session. Clinical visits, ECG and VO2max determination were scheduled for all athletes during thew training period. A group of 18 untrained healthy volunteers matched for age, gender, and body surface area was included as controls. SAECG was acquired in the resting supine position for 15 min and processed to extract average RR interval...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Long-distance runners; Signal-averaged electrocardiogram; Maximal aerobic power; Mathematical model.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200006

Glutamatergic neurotransmission modulates hypoxia-induced hyperventilation but not anapyrexia
Provedor de dados: 56
Autores: Paula,P.M. de; Branco,L.G.S.
The interaction between pulmonary ventilation (V E) and body temperature (Tb) is essential for O2 delivery to match metabolic rate under varying states of metabolic demand. Hypoxia causes hyperventilation and anapyrexia (a regulated drop in Tb), but the neurotransmitters responsible for this interaction are not well known. Since L-glutamate is released centrally in response to peripheral chemoreceptor stimulation and glutamatergic receptors are spread in the central nervous system we tested the hypothesis that central L-glutamate mediates the ventilatory and thermal responses to hypoxia. We measured V E and Tb in 40 adult male Wistar rats (270 to 300 g) before and after intracerebroventricular injection of kynurenic acid (KYN, an ionotropic glutamatergic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kynurenic acid --&gt; Alpha-Methyl-4-carboxyphenyl-glycine (MCPG) Hypoxia Ventilation Body temperature.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000019

Incorporation of dietary trans monounsaturated fatty acids into tissues of Walker 256 tumor-bearing rats
Provedor de dados: 56
Autores: Moreira,N.X.; Curi,R.; Padovese,R.; Mancini-Filho,J.
The correlation between dietary trans fatty acids and neoplasia was examined in the present study. Walker 256 tumor-bearing and control rats were fed a trans monounsaturated fatty acid (MUFA)-rich diet for 8 weeks and the incorporation of trans fatty acids by tumor tissue was examined. Also, the effect of tumor growth on trans fatty acid composition of plasma and liver, and the content of thiobarbituric acid-reactive substances (TBARS) was determined. Walker 256 tumor cells presented both trans and cis MUFAs given in the diet. The equivalent diet proportions were 0.66 for trans and 1.14 for cis. Taking into consideration the proportion of trans MUFAs in plasma (11.47%), the tumor incorporated these fatty acids in a more efficient manner (18.27%) than the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trans fatty acids; Walker 256 tumor; Fatty acid composition; Lipid peroxidation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400010

Comparison of three PET methods to assess peritoneal membrane transport
Provedor de dados: 56
Autores: Romani,RF.; Waniewski,J.; Kruger,L.; Lindholm,B.; Nascimento,M.M.
The peritoneal equilibration test (PET) is the most widespread method for assessing water and solute transport across the peritoneal membrane. This study compared three methods: traditional PET (t-PET), mini-PET, and modified PET (mod-PET). Non-diabetic adults (n=21) who had been on peritoneal dialysis (PD) for at least three months underwent t-PET (glucose 2.5%-4 h), mini-PET (glucose 3.86%-1 h), and mod-PET (glucose 3.86%-4 h) to determine dialysate-to-plasma concentration ratio (D/P) for creatinine and dialysate-to-baseline dialysate concentration ratio (D/D0) for glucose. Agreement between methods regarding D/P creatinine and D/D0 glucose was assessed using analysis of variance (ANOVA), Pearson's correlation coefficient, and Bland-Altman analysis. D/P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peritoneal dialysis; Peritoneal membrane; Peritoneal equilibration test; Dialysis adequacy; Peritoneal solute transport.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800607

Laboratory animal: biological reagent or living being?
Provedor de dados: 56
Autores: Cardoso,C.V.P.; Almeida,A.E.C.C. de.
The duties of humans toward non-human animals and their rights in society have been debated for a long time. However, a discussion on the terminology used for the identification of laboratory animals is usually not considered, although the employment of inadequate terminology may generate disastrous consequences for the animals before, during, and after the experiment. This study intends to defend the use of appropriate terminology, call attention to an unethical attitude of certain professionals when dealing with experimental animals, and also propose operational mechanisms, which allow for those distortions to be corrected.
Palavras-chave: Ethics; Laboratory animal; Terminology; Animal experimentation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100019

The effects of cyclopiazonic acid on intracellular Ca2+ in aortic smooth muscle cells from DOCA-hypertensive rats
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/other
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200016

Pollen mother cells of Tradescantia clone 4430 and Tradescantia pallida var. purpurea are equally sensitive to the clastogenic effects of X-rays
Provedor de dados: 56
Autores: Suyama,F.; Guimarães,E.T.; Lobo,D.-J.A.; Rodrigues,G.S.; Domingos,M.; Alves,E.S.; Carvalho,H.A.; Saldiva,P.H.N.
The Tradescantia micronucleus test is a sensitive bioassay for mutagenesis that may be employed both under field and laboratory conditions. This test has been standardized mostly on the basis of the results obtained with clone 4430. However, this clone is not well adapted to tropical weather, frequently showing problems with growth and flowering. In addition, it is attacked by parasites and insects, a fact that limits its use in field studies aiming at the biomonitoring of air pollution. In the city of São Paulo, Tradescantia pallida (Rose) Hunt. var. purpurea Boom is widely distributed as an ornamental plant in gardens and along roadsides and streets, mostly because of its natural resistance and its easy propagation. In this report, we present...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tradescantia; Micronucleus; Environmental mutagenesis; Clastogenesis; X-Radiation; Pollution biomonitoring.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100018

HBV vaccination of HCV-infected patients with occult HBV infection and anti-HBc-positive blood donors
Provedor de dados: 56
Autores: Pereira,J.S.F.; Gonçales,N.S.L.; Silva,C.; Lazarini,M.S.K.; Pavan,M.H.P.; Fais,V.C.; Gonçales Júnior,F.L.
Anti-HBc positivity is a frequent cause of donation rejection at blood banks. Hepatitis B virus (HBV) infection may also occur in HBsAg-negative patients, a situation denoted occult infection. Similarly, very low levels of HBV-DNA have also been found in the sera of patients with chronic hepatitis C virus (HCV) infection, even in the absence of serum HBsAg. Initially we searched for HBV-DNA in serum of 100 blood donors and 50 HCV-infected patients who were HBsAg negative/anti-HBc positive by nested-PCR and by an HBV monitor commercial test for HBV-DNA. Anti-HBs seroconversion rates were measured in 100 blood donors and in 22 patients with chronic HCV infection after HBV vaccination to determine if the HBV vaccination could eliminate an occult HBV infection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymerase chain reaction; Anti-HBc-positive patients; HCV co-infection; HBV vaccination; Occult HBV infection.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400013

Neuroprotection by flavonoids
Provedor de dados: 56
Autores: Dajas,F.; Rivera-Megret,F.; Blasina,F.; Arredondo,F.; Abin-Carriquiry,J.A.; Costa,G.; Echeverry,C.; Lafon,L.; Heizen,H.; Ferreira,M.; Morquio,A.
The high morbidity, high socioeconomic costs and lack of specific treatments are key factors that define the relevance of brain pathology for human health and the importance of research on neuronal protective agents. Epidemiological studies have shown beneficial effects of flavonoids on arteriosclerosis-related pathology in general and neurodegeneration in particular. Flavonoids can protect the brain by their ability to modulate intracellular signals promoting cellular survival. Quercetin and structurally related flavonoids (myricetin, fisetin, luteolin) showed a marked cytoprotective capacity in in vitro experimental conditions in models of predominantly apoptotic death such as that induced by medium concentrations (200 µM) of H2O2 added to PC12 cells in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flavonoids; Quercetin; Focal ischemia; Neuroprotection.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200002

Antimicrobial peptides and nitric oxide production by neutrophils from periodontitis subjects
Provedor de dados: 56
Autores: Mariano,F.S.; Campanelli,A.P.; Nociti Jr.,F.H.; Mattos-Graner,R.O.; Gonçalves,R.B.
Neutrophils play an important role in periodontitis by producing nitric oxide (NO) and antimicrobial peptides, molecules with microbicidal activity via oxygen-dependent and -independent mechanisms, respectively. It is unknown whether variation in the production of antimicrobial peptides such as LL-37, human neutrophil peptides (HNP) 1-3, and NO by neutrophils influences the pathogenesis of periodontal diseases. We compared the production of these peptides and NO by lipopolysaccharide (LPS)-stimulated neutrophils isolated from healthy subjects and from patients with periodontitis. Peripheral blood neutrophils were cultured with or without Aggregatibacter actinomycetemcomitans-LPS (Aa-LPS), Porphyromonas gingivalis-LPS (Pg-LPS) and Escherichia coli-LPS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial peptides; Nitric oxide; Neutrophils; Periodontitis; Innate immunity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100004

Antidepressant-like effect of lead in adult mice
Provedor de dados: 56
Autores: Mantovani,M.; Matteussi,A.S.; Rodrigues,A.L.S.
It has been reported that lead can cause behavioral impairment by inhibiting the N-methyl-D-aspartate (NMDA) receptor complex. MK-801, a noncompetitive NMDA receptor antagonist, exhibits an antidepressant-like action in the forced swimming test. The purpose of the present study was to determine whether subacute lead exposure in adult male Swiss mice weighing 30-35 g causes an antidepressant-like action in a forced swimming test. Mice were injected intraperitoneally (ip) with 10 mg/kg lead acetate or saline daily for 7 consecutive days. Twenty-four hours after the last treatment, the saline and lead-treated mice received an injection of MK-801 (0.01 mg/kg, ip) or saline and were tested in forced swimming and in open-field tests. Immobility time was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lead acetate; Forced swimming test; Depression; Open-field.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200017

Endothelium-dependent vasodilation in response to Pseudomonas aeruginosa lipopolysaccharide: an in vitro study on canine arteries
Provedor de dados: 56
Autores: Evora,P.R.B.; Ekin,S.; Pearson,P.J.; Schaff,H.V.
Early systemic arterial hypotension is a common clinical feature of Pseudomonas septicemia. To determine if Pseudomonas aeruginosa endotoxin induces the release of endothelium-derived nitric oxide (EDNO), an endogenous nitrovasodilator, segments of canine femoral, renal, hepatic, superior mesenteric, and left circumflex coronary arteries were suspended in organ chambers (physiological salt solution, 95% O2/5% CO2, pH 7.4, 37oC) to measure isometric force. In arterial segments contracted with 2 µM prostaglandin F2&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;, Pseudomonas endotoxin (lipopolysaccharide (LPS) serotype 10(Habs) from Pseudomonas aeruginosa (0.05 to 0.50 mg/ml)) induced concentration-dependent relaxation of segments with endothelium (P&amp;lt;0.05)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelium-derived relaxing factor; EDRF; Nitric oxide; Hypotension; Sepsis.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000014

Renovascular hypertension increases serum TNF and CX3CL1 in experimental Trypanosoma cruzi infection
Provedor de dados: 56
Autores: Silva,M.C.; Azevedo,M.A.; Figueiredo,V.P.; Moura Junior,M.R.; Coelho Junior,D.; Martinelli,P.M.; Machado,R.P.; Alzamora,A.C.; Talvani,A.
Trypanosoma cruzi triggers a progressive inflammatory response affecting cardiovascular functions in humans and experimental models. Angiotensin II, a key effector of the renin-angiotensin system, plays roles in mediating hypertension, heart failure, and inflammatory responses. T. cruzi and AngII can induce inflammatory responses by releasing inflammatory mediators. The aim of this study was to evaluate systemic AngII, tumor necrosis factor (TNF), and CX3CL1 mediators in a two-kidney one-clip (2K1C) renovascular hypertension model using Wistar rats infected with T. cruzi. Our data showed an increase in serum AngII in uninfected and T. cruzi-infected rats 1 week after 2K1C surgery compared to non-2K1C (Sham) animals. The baseline systolic blood pressure was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Hypertension; Tumor necrosis factor; CX3CL1; Cardiac inflammation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500614

Examining life-course influences on chronic disease: the Ribeirão Preto and São Luís birth cohort studies (Brazil)
Provedor de dados: 56
Autores: Batty,G.D.
More than any other low- and middle-income country, Brazil has the longest research tradition of establishing, maintaining and exploiting birth cohort studies. This research pedigree is highlighted in the present issue of the Brazilian Journal of Medical and Biological Research, which contains a series of twelve papers from the Ribeirão Preto and São Luis birth cohort studies from the Southeast and Northeast of Brazil, respectively. The topics covered in this raft of reports vary and include predictors of perinatal health and maternal risk factors, early life determinants of cardiovascular risk factors in childhood and adolescence, use of health services, and a description of dietary characteristics of young adults, amongst other topics. There is also a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Birth cohort; Chronic disease; Developing country; Epidemiology; Life-course.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900001

Antinociceptive properties in mice of a lectin isolated from the marine alga Amansia multifida Lamouroux
Provedor de dados: 56
Autores: Neves,S.A.; Freitas,A.L.P.; Souza,B.W.S.; Rocha,M.L.A.; Correia,M.V.O.; Sampaio,D.A.; Viana,G.S.B.
The antinociceptive effects of a lectin (LEC) isolated from the marine alga Amansia multifida were determined in Swiss mice. The LEC (1, 5, and 10 mg/kg) inhibited acetic acid-induced abdominal writhings in a dose-dependent manner after intraperitoneal or oral administration. A partial but significant inhibition of writhings was observed after the combination of LEC (10 mg/kg) with avidin (1 mg/kg), a potent inhibitor of the hemmaglutinant activity of the lectin. However, total writhing inhibition was demonstrable in the group of mice treated with LEC plus mannose (1 mg/kg), as compared to LEC alone or to control groups. Furthermore, avidin and mainly mannose also play a role in antinociception, somehow facilitating the interaction of LEC with its active...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Red algae; Amansia multifida; Lectin; Antinociceptive effects; Mannose-specific lectin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100016

Ultrasound method applied to characterize healthy femoral diaphysis of Wistar rats in vivo
Provedor de dados: 56
Autores: Fontes-Pereira,A.; Matusin,D.P.; Rosa,P.; Schanaider,A.; von Krüger,M.A.; Pereira,W.C.A.
A simple experimental protocol applying a quantitative ultrasound (QUS) pulse-echo technique was used to measure the acoustic parameters of healthy femoral diaphyses of Wistar rats in vivo. Five quantitative parameters [apparent integrated backscatter (AIB), frequency slope of apparent backscatter (FSAB), time slope of apparent backscatter (TSAB), integrated reflection coefficient (IRC), and frequency slope of integrated reflection (FSIR)] were calculated using the echoes from cortical and trabecular bone in the femurs of 14 Wistar rats. Signal acquisition was performed three times in each rat, with the ultrasound signal acquired along the femur's central region from three positions 1 mm apart from each other. The parameters estimated for the three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone; Tissue characterization; Quantitative ultrasound; Clinical research protocols; Animal model.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500403

Prevalence of Y chromosome deletions in a Brazilian population of nonobstructive azoospermic and severely oligozoospermic men
Provedor de dados: 56
Autores: SãoPedro,S.L.; Fraietta,R.; Spaine,D.; Porto,C.S.; Srougi,M.; Cedenho,A.P.; Avellar,M.C.W.
We determined the prevalence of Y chromosome deletions in a population of 60 Brazilian nonobstructive azoospermic and severely oligozoospermic men. PCR-based screening of microdeletions was performed on lymphocyte DNA for the presence of 14 sequence-tagged sites (STS) located in the azoospermic factor (AZF) on the Yq chromosome. All STS were amplified efficiently in samples from 12 fertile men tested, but failed to be amplified in samples from fertile women, indicating the specificity of PCR conditions for Yq screening. Overall, 4 of the 60 infertile patients tested (6.7%) exhibited deletion of the Y chromosome, 2 of them being severely oligozoospermic patients (P10 and P32) and 2 azoospermic men (patients P47 and P57). Patients P47 and P57 presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Y chromosome; Microdeletions; Severe oligozoospermia; Azoospermia; Male infertility; Azoospermia factor; AZF region.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600015

Lipolysis of emulsion models of triglyceride-rich lipoproteins is altered in male patients with abdominal aorta aneurysm
Provedor de dados: 56
Autores: Hosni,J.J.; Vinagre,C.G.; Mady,C.; Maranhão,R.C.
Disorders of the lipid metabolism may play a role in the genesis of abdominal aorta aneurysm. The present study examined the intravascular catabolism of chylomicrons, the lipoproteins that carry the dietary lipids absorbed by the intestine in the circulation in patients with abdominal aorta aneurysm. Thirteen male patients (72 ± 5 years) with abdominal aorta aneurysm with normal plasma lipid profile and 13 healthy male control subjects (73 ± 5 years) participated in the study. The method of chylomicron-like emulsions was used to evaluate this metabolism. The emulsion labeled with 14C-cholesteryl oleate and ³H-triolein was injected intravenously in both groups. Blood samples were taken at regular intervals over 60 min to determine the decay curves. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Abdominal aortic aneurysm; Lipoproteins; Chylomicrons; Emulsions.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300004

Why are the rates of cesarean section in Brazil higher in more developed cities than in less developed ones?
Provedor de dados: 56
Autores: Ribeiro,V.S.; Figueiredo,F.P.; Silva,A.A.M.; Bettiol,H.; Batista,R.F.L.; Coimbra,L.C.; Lamy,Z.C.; Barbieri,M.A.
The objective of the present study was to investigate factors associated with cesarean sections in two cities located in different regions of Brazil and to determine factors that explain the higher cesarean section rate in the more developed city, Ribeirão Preto, compared to the less developed one, São Luís. Data from two cohort studies comprising 2846 women in Ribeirão Preto in 1994, and 2443 women in São Luís in 1997/1998 were used. Adjusted and non-adjusted risk estimates were calculated using a Poisson regression model. The cesarean section rate was 33.7% in São Luís and 50.8% in Ribeirão Preto. Adjusted analysis in a joint sequential model revealed a 51% higher risk of cesarean section in Ribeirão Preto compared to São Luís (prevalence rate ratio...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cesarean section; Epidemiology; Obstetrics; Health services; Risk factors.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900008

Increased production of tumor necrosis factor-alpha in whole blood cultures from children with primary malnutrition
Provedor de dados: 56
Autores: Azevedo,Z.M.A.; Luz,R.A.; Victal,S.H.; Kurdian,B.; Fonseca,V.M.; Fitting,C.; Câmara,F.P.; Haeffner-Cavaillon,N.; Cavaillon,J.-M.; Gaspar Elsas,M.I.C.; Xavier Elsas,P.
Because low tumor necrosis factor-alpha (TNF-alpha) production has been reported in malnourished children, in contrast with high production of TNF-alpha in experimental protein-energy malnutrition, we reevaluated the production of TNF-alpha in whole blood cultures from children with primary malnutrition free from infection, and in healthy sex- and age-matched controls. Mononuclear cells in blood diluted 1:5 in endotoxin-free medium released TNF-alpha for 24 h. Spontaneously released TNF-alpha levels (mean ± SEM), as measured by enzyme immunoassay in the supernatants of unstimulated 24-h cultures, were 10,941 ± 2,591 pg/ml in children with malnutrition (N = 11) and 533 ± 267 pg/ml in controls (N = 18) (P &lt; 0.0001). TNF-alpha production was increased by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TNF-alpha; Malnutrition; LPS.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200005

Investigation of single-strand conformational polymorphism of the TP53 gene in women with a family history of breast cancer
Provedor de dados: 56
Autores: Burbano,R.R.; Medeiros,A.C.; Mello,A.A.; Lemos,J.A.; Bahia,M.O.; Casartelli,C.
Breast cancer in families with germ line mutations in the TP53 gene has been described in the medical literature. Mutation screening for susceptibility genes should allow effective prophylactic and preventive measures. Using single-strand conformational polymorphism, we screened for mutations in exons 5, 6, 7 and 8 of gene TP53 in the peripheral blood of 8 young non-affected members (17 to 36 years old) of families with a history of breast cancer. Studies of this type on young patients (mean age, 25 years) are very rare in the literature. The identification of these mutations would contribute to genetic counseling of members of families with predisposition to breast cancer. The results obtained did not show any polymorphism indicating mutation. In our...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: TP53; Family history of breast cancer; Mutation screening; Early breast cancer.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100010

Role of nuclear factor kappa B and reactive oxygen species in the tumor necrosis factor-a-induced epithelial-mesenchymal transition of MCF-7 cells
Provedor de dados: 56
Autores: Dong,R.; Wang,Q.; He,X.L.; Chu,Y.K.; Lu,J.G.; Ma,Q.J.
The microenvironment of the tumor plays an important role in facilitating cancer progression and activating dormant cancer cells. Most tumors are infiltrated with inflammatory cells which secrete cytokines such as tumor necrosis factor-a (TNF-a). To evaluate the role of TNF-a in the development of cancer we studied its effects on cell migration with a migration assay. The migrating cell number in TNF-a -treated group is about 2-fold of that of the control group. Accordingly, the expression of E-cadherin was decreased and the expression of vimentin was increased upon TNF-a treatment. These results showed that TNF-a can promote epithelial-mesenchymal transition (EMT) of MCF-7 cells. Further, we found that the expression of Snail, an important transcription...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tumor necrosis factor-a; Nuclear factor kappa B; Reactive oxygen species; Snail; E-cadherin; Epithelial-mesenchymal transition.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800007

Protein 3-nitrotyrosine formation during Trypanosoma cruzi infection in mice
Provedor de dados: 56
Autores: Naviliat,M.; Gualco,G.; Cayota,A.; Radi,R.
Nitric oxide (·NO) is a diffusible messenger implicated in Trypanosoma cruzi resistance. Excess production of ·NO and oxidants leads to the generation of nitrogen dioxide (·NO2), a strong nitrating agent. Tyrosine nitration is a post-translational modification resulting from the addition of a nitro (-NO2) group to the ortho-position of tyrosine residues. Detection of protein 3-nitrotyrosine is regarded as a marker of nitro-oxidative stress and is observed in inflammatory processes. The formation and role of nitrating species in the control and myocardiopathy of T. cruzi infection remain to be studied. We investigated the levels of ·NO and protein 3-nitrotyrosine in the plasma of C3H and BALB/c mice and pharmacologically modulated their production during...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peroxynitrite; Nitric oxide; Nitrotyrosine; Free radicals; Trypanosoma cruzi; Chagas' disease.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200011

Lack of antidiabetic effect of a Eugenia jambolana leaf decoction on rat streptozotocin diabetes
Provedor de dados: 56
Autores: Pepato,M.T.; Folgado,V.B.B.; Kettelhut,I.C.; Brunetti,I.L.
Streptozotocin-diabetic rats were treated for 17 days with a decoction of Eugenia jambolana (Myrtaceae) leaves (15%, w/v) as a substitute for water. Body weight, food and fluid intake, urine volume, glycemia, urinary glucose and urea were evaluated every 5 days. The animals were sacrificed by decapitation and blood samples collected for the determination of glycemia, serum cholesterol, HDL-cholesterol, triglycerides and angiotensin-converting enzyme. The weight of adipose and muscle tissues was also determined. There were no statistically significant differences between treated and untreated rats for any of the biochemical or physiological parameters. We conclude that, at least in this experimental model, Eugenia jambolana leaf decoction has no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antidiabetic plant; Hyperglycemia; Diabetic rats; Eugenia jambolana.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300014

Swimming training increases cardiac vagal activity and induces cardiac hypertrophy in rats
Provedor de dados: 56
Autores: Medeiros,A.; Oliveira,E.M.; Gianolla,R.; Casarini,D.E.; Negrão,C.E.; Brum,P.C.
The effect of swimming training (ST) on vagal and sympathetic cardiac effects was investigated in sedentary (S, N = 12) and trained (T, N = 12) male Wistar rats (200-220 g). ST consisted of 60-min swimming sessions 5 days/week for 8 weeks, with a 5% body weight load attached to the tail. The effect of the autonomic nervous system in generating training-induced resting bradycardia (RB) was examined indirectly after cardiac muscarinic and adrenergic receptor blockade. Cardiac hypertrophy was evaluated by cardiac weight and myocyte morphometry. Plasma catecholamine concentrations and citrate synthase activity in soleus muscle were also determined in both groups. Resting heart rate was significantly reduced in T rats (355 ± 16 vs 330 ± 20 bpm). RB was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swimming-training exercise; Cardiac autonomic balance; Hemodynamics; Hypertrophic effect; Parasympathetic system; Resting bradycardia.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200018

Oculo-facio-cardio-dental syndrome in three succeeding generations: genotypic data and phenotypic features
Provedor de dados: 56
Autores: Lozić,B.; Ljubković,J.; Pandurić,D. Gabrić; Saltvig,I.; Kutsche,K.; Krželj,V.; Zemunik,T.
Oculo-facio-cardio-dental (OFCD) syndrome is a rare X-linked disorder mainly manifesting in females. Patients show ocular, facial, cardiac, and dental abnormalities. OFCD syndrome is caused by heterozygous mutations in the BCOR gene, located in Xp11.4, encoding the BCL6 co-repressor. We report a Croatian family with four female members (grandmother, mother and monozygotic female twins) diagnosed with OFCD syndrome who carry the novel BCOR mutation c.4438C&gt;T (p.R1480*). They present high intrafamilial phenotypic variability with special regard to cardiac defect and cataract that showed more severe disease expression in successive generations. Clinical and radiographic examination of the mother of the twins revealed a talon cusp involving the permanent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: OFCD syndrome; BCOR gene; Talon cusp; Intrafamilial phenotypic variability.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200030

Three days of intermittent stretching after muscle disuse alters the proteins involved in force transmission in muscle fibers in weanling rats
Provedor de dados: 56
Autores: Gianelo,M.C.S.; Polizzelo,J.C.; Chesca,D.; Mattiello-Sverzut,A.C.
The aim of this study was to determine the effects of intermittent passive manual stretching on various proteins involved in force transmission in skeletal muscle. Female Wistar weanling rats were randomly assigned to 5 groups: 2 control groups containing 21- and 30-day-old rats that received neither immobilization nor stretching, and 3 test groups that received 1) passive stretching over 3 days, 2) immobilization for 7 days and then passive stretching over 3 days, or 3) immobilization for 7 days. Maximal plantar flexion in the right hind limb was imposed, and the stretching protocol of 10 repetitions of 30 s stretches was applied. The soleus muscles were harvested and processed for HE and picrosirius staining; immunohistochemical analysis of collagen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Vimentin; Desmin; Intermittent stretching; Collagen.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200603

Ingestion of a moderate dose of alcohol enhances physical exercise-induced changes in blood lactate concentration
Provedor de dados: 56
Autores: Teixeira-Coelho,F.; Santos,D.F.C.; Santos,G.A.; Sousa,T.F.; Moreira,S.R.; Souza,M.V.C.; Wanner,S.P.
The consumption of alcoholic beverages influences carbohydrate and lipid metabolism, although it is not yet clear whether metabolism during physical exercise at different intensities is also affected. This was the objective of the present study. Eight young and healthy volunteers performed a treadmill test to identify the running speed corresponding to a lactate concentration of 4 mM (S4mM). At least 48 h later, they were subjected to two experimental trials (non-alcohol or alcohol) in which they performed two 1-km running sessions at the following intensities: 1) S4mM; 2) 15% above S4mM. In both trials, blood lactate, triglycerides, and glucose concentrations were measured before and after exercise. The acute alcohol intake increased triglycerides, but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endurance; Ethanol; Glucose; Lactate; Metabolism; Treadmill running.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400601

Ox-LDL increases OX40L in endothelial cells through a LOX-1-dependent mechanism
Provedor de dados: 56
Autores: Dong,Q.; Xiang,R.; Zhang,D.Y.; Qin,S.
Oxidative low-density lipoprotein (Ox-LDL) is a key risk factor for the development of atherosclerosis, and it can stimulate the expression of a variety of inflammatory signals. As a new and highly sensitive inflammation index, OX40L may be a key to understanding the mechanisms that regulate interactions between cells within the vessel wall and inflammatory mediators during the development of atherosclerosis. To investigate whether Ox-LDL regulates OX40L expression through an oxidized LDL-1 receptor (LOX-1)-mediated mechanism, we investigated the effect of different concentrations of Ox-LDL (50, 100, 150 µg/mL) on endothelial cell proliferation and apoptosis. Stimulation with Ox-LDL increased OX40L protein 1.44-fold and mRNA 4.0-fold in endothelial cells,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Ox-LDL; LOX-1; OX40/OX40L; Polyinosinic acid.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900765

Sleep, ageing and night work
Provedor de dados: 56
Autores: Pires,M.L.N.; Teixeira,C.W.; Esteves,A.M.; Bittencourt,L.R.A.; Silva,R.S.; Santos,R.F.; Tufik,S.; Mello,M.T.
Studies have shown that the frequency or worsening of sleep disorders tends to increase with age and that the ability to perform circadian adjustments tends to decrease in individuals who work the night shift. This condition can cause consequences such as excessive sleepiness, which are often a factor in accidents that occur at work. The present study investigated the effects of age on the daytime and nighttime sleep patterns using polysomnography (PSG) of long-haul bus drivers working fixed night or day shifts. A total of 124 drivers, free of sleep disorders and grouped according to age (&lt;45 years, N = 85, and ≥45 years, N = 39) and PSG timing (daytime (D) PSG, N = 60; nighttime (N) PSG, N = 64) participated in the study. We observed a significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep; Shift work; Night work; Aging; Polysomnography; REM; Slow wave sleep (SWS).
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900011

Trypan blue exclusion assay by flow cytometry
Provedor de dados: 56
Autores: Avelar-Freitas,B.A.; Almeida,V.G.; Pinto,M.C.X.; Mourão,F.A.G.; Massensini,A.R.; Martins-Filho,O.A.; Rocha-Vieira,E.; Brito-Melo,G.E.A.
Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the expertise of the analyst, can affect the results. We propose an alternative assay for evaluating cell viability that combines the TB exclusion test and the high sensitivity of the flow cytometry technique. Previous studies have demonstrated the ability of TB to emit fluorescence when complexed with proteins. According to our results, TB/bovine serum albumin and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypan blue; Fluorescence; Flow cytometry; Cytotoxicity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400307

Ventilation with high tidal volume induces inflammatory lung injury
Provedor de dados: 56
Autores: Bueno,P.C.S.; Bueno,C.E.; Santos,M.L.; Oliveira-Júnior,I.; Salomão,R.; Pinheiro,B.V.; Beppu,O.S.
Mechanical ventilation with high tidal volumes (V T) has been shown to induce lung injury. We examined the hypothesis that this procedure induces lung injury with inflammatory features. Anesthetized male Wistar rats were randomized into three groups: group 1 (N = 12): V T = 7 ml/kg, respiratory rate (RR) = 50 breaths/min; group 2 (N = 10): V T = 21 ml/kg, RR = 16 breaths/min; group 3 (N = 11): V T = 42 ml/kg, RR = 8 breaths/min. The animals were ventilated with fraction of inspired oxygen of 1 and positive end-expiratory pressure of 2 cmH2O. After 4 h of ventilation, group 3, compared to groups 1 and 2, had lower PaO2 [280 (range 73-458) vs 517 (range 307-596), and 547 mmHg (range 330-662), respectively, P&lt;0.05], higher wet lung weight [3.62 ± 0.91 vs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung injury; Mechanical ventilation; Inflammation; TNF-alpha.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200007

Diabetes mellitus affects the biomechanical function of the callus and the expression of TGF-beta1 and BMP2 in an early stage of fracture healing
Provedor de dados: 56
Autores: Xu,M.T.; Sun,S.; Zhang,L.; Xu,F.; Du,S.L.; Zhang,X.D.; Wang,D.W.
Transforming growth factor beta 1 (TGF-β1) and bone morphogenetic protein-2 (BMP-2) are important regulators of bone repair and regeneration. In this study, we examined whether TGF-β1 and BMP-2 expressions were delayed during bone healing in type 1 diabetes mellitus. Tibial fractures were created in 95 diabetic and 95 control adult male Wistar rats of 10 weeks of age. At 1, 2, 3, 4, and 5 weeks after fracture induction, five rats were sacrificed from each group. The expressions of TGF-β1 and BMP2 in the fractured tibias were measured by immunohistochemistry and quantitative reverse-transcription polymerase chain reaction, weekly for the first 5 weeks post-fracture. Mechanical parameters (bending rigidity, torsional rigidity, destruction torque) of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Biomechanical function; TGF-beta1; BMP-2; Fracture healing; Mechanical parameters.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100604

Antioxidant activity of the microalga Spirulina maxima
Provedor de dados: 56
Autores: Miranda,M.S.; Cintra,R.G.; Barros,S.B.M.; Mancini-Filho,J.
Spirulina maxima, which is used as a food additive, is a microalga rich in protein and other essential nutrients. Spirulina contains phenolic acids, tocopherols and ß-carotene which are known to exhibit antioxidant properties. The aim of the present study was to evaluate the antioxidant capacity of a Spirulina extract. The antioxidant activity of a methanolic extract of Spirulina was determined in vitro and in vivo. The in vitro antioxidant capacity was tested on a brain homogenate incubated with and without the extract at 37oC. The IC50 (concentration which causes a 50% reduction of oxidation) of the extract in this system was 0.18 mg/ml. The in vivo antioxidant capacity was evaluated in plasma and liver of animals receiving a daily dose of 5 mg for 2 and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Microalgae antioxidant activity Spirulina maxima phenolic compounds ß-carotene &lt; FONT FACE=Symbol&gt; A&lt; /FONT&gt; -tocopherol.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800007

Notch ligand Delta-like 1 promotes the metastasis of melanoma by enhancing tumor adhesion
Provedor de dados: 56
Autores: Zhang,J.P.; Li,N.; Bai,W.Z.; Qiu,X.C.; Ma,B.A.; Zhou,Y.; Fan,Q.Y.; Shan,L.Q.
Notch signaling plays a vital role in tumorigenicity and tumor progression by regulating proliferation, invasion, and the tumor microenvironment. Previous research by our group indicated that Notch ligand Delta-like 1 (Dll1) is involved in angiogenesis in melanoma, and we noticed that it took a longer time to trypsinize Dll1-expressing B16 melanoma cells than the control cells. In this article, we extended our study to investigate the effects of Dll1 on tumor cell adhesion and metastasis. Dll1 overexpression activated Notch signaling in B16 tumor cells and significantly enhanced the adhering capacity of B16 tumor cells both in vitro and in vivo. B16-Dll1 cells also had a higher metastatic potential than their counterpart in the mouse model of lung...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Delta-like 1; Notch signaling; Melanoma; Metastasis; Adhesion; N-cadherin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400299

Immunization by subcutaneous implants of polyester-polyurethane sponges coupled with antigen
Provedor de dados: 56
Autores: Lima,S.L.; Machado,C.B.; Pereira,M.A.C.; Cara,D.C.; Velarde,D.T.; Andrade,S.P.; Gontijo,C.M.
A new protocol is described for immunization of outbred Swiss mice. The procedure is based on subcutaneous implantation of antigen-coupled polyester-polyurethane sponges cut into disks of 10 mm in diameter vs 2 mm in thickness. Antigen coupling was performed by overnight incubation of the sponge with a solution of ovalbumin (Ova) (2 mg/ml) diluted in sodium carbonate buffer, pH 9.6. The amount of ovalbumin that was taken up by the sponge was between 71.4 to 82.5 µg. This was estimated by comparing the Ova absorbance at 280 nm in coating buffer solutions before and after incubation. To compare the efficiency of the proposed method, experimental groups immunized with the antigen in the presence of adjuvants (10 µg in Al(OH)3 or 100 µg in complete Freund's...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sponge; Implant; Immunization; Vaccine.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400011

Long non-coding RNA-ROR aggravates myocardial ischemia/reperfusion injury
Provedor de dados: 56
Autores: Zhang,Weiwei; Li,Ying; Wang,Peng.
Long non-coding RNAs (lncRNAs) play an important role in the pathogenesis of cardiovascular diseases, especially in myocardial infarction and ischemia/reperfusion (I/R). However, the underlying molecular mechanism remains unclear. In this study, we determined the role and the possible underlying molecular mechanism of lncRNA-ROR in myocardial I/R injury. H9c2 cells and human cardiomyocytes (HCM) were subjected to either hypoxia/reoxygenation (H/R), I/R or normal conditions (normoxia). The expression levels of lncRNA-ROR were detected in serum of myocardial I/R injury patients, H9c2 cells, and HCM by qRT-PCR. Then, levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) were measured by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LncRNA; Ischemia/reperfusion (I/R); Hypoxia/reoxygenation (H/R); Cell viability; Apoptosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600609

The immunomodulator glatiramer acetate influences spinal motoneuron plasticity during the course of multiple sclerosis in an animal model
Provedor de dados: 56
Autores: Marques,K.B.; Scorisa,J.M.; Zanon,R.; Freria,C.M.; Santos,L.M.B.; Damasceno,B.P.; Oliveira,A.L.R.
The immunomodulador glatiramer acetate (GA) has been shown to significantly reduce the severity of symptoms during the course of multiple sclerosis and in its animal model - experimental autoimmune encephalomyelitis (EAE). Since GA may influence the response of non-neuronal cells in the spinal cord, it is possible that, to some extent, this drug affects the synaptic changes induced during the exacerbation of EAE. In the present study, we investigated whether GA has a positive influence on the loss of inputs to the motoneurons during the course of EAE in rats. Lewis rats were subjected to EAE associated with GA or placebo treatment. The animals were sacrificed after 15 days of treatment and the spinal cords processed for immunohistochemical analysis and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental autoimmune encephalomyelitis; Spinal cord; Synapse elimination; Motoneuron; Immunomodulator; Glatiramer acetate.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200006

Angiotensin II induces NF-κB, JNK and p38 MAPK activation in monocytic cells and increases matrix metalloproteinase-9 expression in a PKC- andRho kinase-dependent manner
Provedor de dados: 56
Autores: Yaghooti,H.; Firoozrai,M.; Fallah,S.; Khorramizadeh,M.R.
Angiotensin II (ANG II), the main effector of the renin-angiotensin system, is implicated in endothelial permeability, recruitment and activation of the immune cells, and also vascular remodeling through induction of inflammatory genes. Matrix metalloproteinases (MMPs) are considered to be important inflammatory factors. Elucidation of ANG II signaling pathways and of possible cross-talks between their components is essential for the development of efficient inhibitory medications. The current study investigates the inflammatory signaling pathways activated by ANG II in cultures of human monocytic U-937 cells, and the effects of specific pharmacological inhibitors of signaling intermediates on MMP-9 gene (MMP-9) expression and activity. MMP-9 expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; Signaling; MMP-9; Monocytic cell.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300003

Dendritic thickness: a morphometric parameter to classify mouse retinal ganglion cells
Provedor de dados: 56
Autores: Loopuijt,L.D.; Silva Filho,M. da; Hirt,B.; Vonthein,R.; Kremers,J.
To study the dendritic morphology of retinal ganglion cells in wild-type mice we intracellularly injected these cells with Lucifer yellow in an in vitro preparation of the retina. Subsequently, quantified values of dendritic thickness, number of branching points and level of stratification of 73 Lucifer yellow-filled ganglion cells were analyzed by statistical methods, resulting in a classification into 9 groups. The variables dendritic thickness, number of branching points per cell and level of stratification were independent of each other. Number of branching points and level of stratification were independent of eccentricity, whereas dendritic thickness was positively dependent (r = 0.37) on it. The frequency distribution of dendritic thickness tended...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ganglion cell morphology; Retinal circuitry; Intracellular injection of neurotracers; Lucifer yellow; Dendritic thickness.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000010

Epigallocatechin-3-gallate ameliorates lipopolysaccharide-induced acute lung injury by suppression of TLR4/NF-κB signaling activation
Provedor de dados: 56
Autores: Wang,Jia; Fan,Shi Ming; Zhang,Jiong.
Acute lung injury (ALI) is a serious clinical syndrome with a high rate of mortality. The activation of inflammation is well-recognized as a vital factor in the pathogenesis of lipopolysaccharide (LPS)-induced ALI. Therefore, suppression of the inflammatory response could be an ideal strategy to prevent ALI. Epigallocatechin-3-gallate (EGCG), mainly from green tea, has been shown to have an anti-inflammatory effect. The aim of the study was to explore whether EGCG alleviates inflammation in sepsis-related ALI. Male BALB/C mice were treated with EGCG (10 mg/kg) intraperitoneally (ip) 1 h before LPS injection (10 mg/kg, ip). The results showed that EGCG attenuated LPS-induced ALI as it decreased the changes in blood gases and reduced the histological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epigallocatechin-3-gallate; Acute lung injury; Inflammation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700605

Apoptosis of sensory neurons and satellite cells after sciatic nerve transection in C57BL/6J mice
Provedor de dados: 56
Autores: Oliveira,A.L.R.
The rate of axonal regeneration, after sciatic nerve lesion in adult C57BL/6J mice, is reduced when compared to other isogenic strains. It was observed that such low regeneration was not due just to a delay, since neuronal death was observed. Two general mechanisms of cell death, apoptosis and necrosis, may be involved. By using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) technique, we demonstrated that a large number of sensory neurons, as well as satellite cells found in the dorsal root ganglia, were intensely labeled, thus indicating that apoptotic mechanisms were involved in the death process. Although almost no labeled neurons or satellite cells were observed one week after transection, a more than ten-fold increase in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: TUNEL; Apoptosis; Axotomy; Neuronal death; Satellite cells; Isogenic mice.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300012

Serum cytokines in patients with Brazilian pemphigus foliaceus (fogo selvagem)
Provedor de dados: 56
Autores: Zeoti,D.M.; Figueiredo,J.F.C.; Chiossi,M.P.V.; Roselino,A.M.F.
Pemphigus foliaceus (PF) is characterized by acantholysis determined by IgG4 binding to desmoglein I, a 160-kDa desmosomal glycoprotein. To investigate the immunopathological aspects of Brazilian PF, we determined levels of serum cytokines in patients with PF. Twenty-five patients with PF and a control group consisting of 10 healthy individuals were studied. Serum IL-2, IL-4, IL-5, IL-10, IL-12 and IFN-gamma were measured in the two groups by ELISA. The median concentration of IL-2 was lower in PF patients compared to the control group (0.45 and 9.50 pg/ml, respectively), as also was the concentration of IL-4 (0.26 and 10.16 pg/ml, respectively). The same was observed for IL-5 (7.94 and 15.74 pg/ml, respectively) and for IFN-gamma (5.90 and 8.58 pg/ml,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Pemphigus; Pemphigus foliaceus; Fogo selvagem; Bullous dermatoses.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900012

The nucleolus: a paradigm for cell proliferation and aging
Provedor de dados: 56
Autores: Comai,L.
The nucleolus is the cellular site of ribosome biosynthesis. At this site, active ribosomal DNA (rDNA) genes are rapidly transcribed by RNA polymerase I (pol I) molecules. Recent advances in our understanding of the pol I transcription system have indicated that regulation of ribosomal RNA (rRNA) synthesis is a critical factor in cell growth. Importantly, the same signaling networks that control cell growth and proliferation and are deregulated in cancer appear to control pol I transcription. Therefore, the study of the biochemical basis for growth regulation of pol I transcription can provide basic information about the nuclear signaling network. Hopefully, this information may facilitate the search for drugs that can inhibit the growth of tumor cells by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nucleolus; RNA pol I; Aging; Transcription; Werner syndrome.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200004

Hormone replacement therapy increases levels of antibodies against heat shock protein 65 and certain species of oxidized low density lipoprotein
Provedor de dados: 56
Autores: Uint,L.; Gebara,O.C.E.; Pinto,L.B.; Wajngarten,M.; Boschcov,P.; Luz,P.L. da; Gidlund,M.
Hormone replacement therapy (HRT) reduces cardiovascular risks, although the initiation of therapy may be associated with transient adverse ischemic and thrombotic events. Antibodies against heat shock protein (Hsp) and oxidized low density lipoprotein (LDL) have been found in atherosclerotic lesions and plasma of patients with coronary artery disease and may play an important role in the pathogenesis of atherosclerosis. The aim of the present study was to assess the effects of HRT on the immune response by measuring plasma levels of antibodies against Hsp 65 and LDL with a low and high degree of copper-mediated oxidative modification of 20 postmenopausal women before and 90 days after receiving orally 0.625 mg equine conjugate estrogen plus 2.5 mg...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hormone replacement therapy; Postmenopause; Oxidized LDL; Heat shock protein; Immune response; Antibodies.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400011

Cholesterol changes the fatty acid composition of rat enterocytes
Provedor de dados: 56
Autores: Gazzola,J.; Martins,E.F.; Miyasaka,C.K.; Palanch,A.C.; Vecchia,M.G.; Curi,R.
The effect of free cholesterol on the fatty acid composition and growth of rat fetal enterocytes was investigated in the absence and presence of 10% (v/v) fetal calf serum. Cholesterol caused a significant reduction of cell number after 6 and 12 h in culture. The fatty acid composition of enterocytes cultured in the presence of serum was also changed by the presence of 20 µM cholesterol. The fatty acid profile was determined by HPLC using fluorescence detection (325 nm excitation and 395 nm emission). Cholesterol (20 µM) increased the proportion (given in percentage of the total fatty acids) of the following fatty acids in cultured cells: lauric (by 42%), oleic (by 34%), linoleic (by 44%) and gamma-linolenic (by 20%) acids and reduced the proportion of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enterocytes; Cholesterol; Fatty acid composition; Cell proliferation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100019

Construction of a fusion plasmid containing the PSCA gene and cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and its anti-tumor effect in an animal model of prostate cancer
Provedor de dados: 56
Autores: Mai,T.J.; Ma,R.; Li,Z.; Bi,S.C.
Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is a negative regulator of T cell activation, which competes with CD28 for B7.1/B7.2 binding, and which has a greater affinity. Fusion of specific antigens to extracellular domain of CTLA4 represents a promising approach to increase the immunogenicity of DNA vaccines. In this study, we evaluated this interesting approach for CTLA4 enhancement on prostate stem cell antigen (PSCA)-specific immune responses and its anti-tumor effects in a prostate cancer mouse model. Consequently, we constructed a DNA vaccine containing the PSCA and the CTLA-4 gene. Vaccination with the CTLA4-fused DNA not only induced a much higher level of anti-PSCA antibody, but also increased PSCA-specific T cell response in mice. To...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; DNA vaccine; PSCA; CTLA-4.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100601

Partial purification of tightly bound mitochondrial hexokinase from maize (Zea mays L.) root membranes
Provedor de dados: 56
Autores: Rezende,G.L.; Logullo,C.; Meyer,L.; Machado,L.B.; Oliveira-Carvalho,A.L.; Zingali,R.B.; Cifuentes,D.; Galina,A.
In mammals, hexokinase (HK) is strategically located at the outer membrane of mitochondria bound to the porin protein. The mitochondrial HK is a crucial modulator of apoptosis and reactive oxygen species generation. In plants, these properties related to HK are unknown. In order to better understand the physiological role of non-cytosolic hexokinase (NC-HK) in plants, we developed a purification strategy here described. Crude extract of 400 g of maize roots (230 mg protein) contained a specific activity of 0.042 µmol G6P min-1 mg PTN-1. After solubilization with detergent two fractions were obtained by DEAE column chromatography, NC-HK 1 (specific activity = 3.6 µmol G6P min-1 mg PTN-1 and protein recovered = 0.7 mg) and NC-HK 2. A major purification...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrophobic interaction chromatography; Maize; Mitochondrial hexokinase; Non-cytosolic hexokinase; Purification.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900003

Clinical classification of tetanus patients
Provedor de dados: 56
Autores: Miranda-Filho,D.B.; Ximenes,R.A.A.; Barone,A.A.; Vaz,V.L.; Vieira,A.G.; Albuquerque,V.M.G.
The authors propose a clinical classification to monitor the evolution of tetanus patients, ranging from grade I to IV according to severity. It was applied on admission and repeated on alternate days up to the 10th day to patients aged  &gt; or = 12 years admitted to the State University Hospital, Recife, Brazil. Patients were also classified upon admission according to three prognostic indicators to determine if the proposed classification is in agreement with the traditionally used indicators. Upon admission, the distribution of the 64 patients among the different levels of the proposed classification was similar for the groups of better and worse prognosis according to the three indicators (P &gt; 0.05), most of the patients belonging to grades I and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tetanus; Progression; Classification; Clinical.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001000009

Effects of pentoxifylline treatment before freezing on motility, viability and acrosome status of poor quality human spermatozoa cryopreserved by the liquid nitrogen vapor method
Provedor de dados: 56
Autores: Esteves,S.C.; Spaine,D.M.; Cedenho,A.P.
The objective of the present study was to investigate the effects of the direct addition of pentoxifylline (PF) to the ejaculates of men with poor sperm quality before freezing on post-thaw sperm motility, viability, acrosome integrity, and agonist-induced acrosome reaction. Semen specimens from 16 infertile men with impaired sperm count and motility (oligoasthenozoospermia) were divided into two equal aliquots: one received no treatment (control) while the other was incubated with 5 mM PF (treated). Both aliquots were cryopreserved by the liquid nitrogen vapor method. Motility was assessed according to WHO criteria. Acrosome integrity and spontaneous and calcium ionophore-induced acrosome reactions were assessed with fluorescein isothiocyanate-conjugated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrosome; Cryopreservation; Human spermatozoa; Ionophore; Pentoxifylline; Oligoasthenozoospermia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700014

Circadian time-dependent effects of fencamfamine on inhibition of dopamine uptake and release in rat striatal slices
Provedor de dados: 56
Fencamfamine (FCF) is a central stimulant that facilitates central dopaminergic transmission through inhibition of dopamine uptake and enhanced release of the transmitter. We evaluated the changes in the inhibition of uptake and the release of striatal [3H]-dopamine at 9:00 and 21:00 h, times corresponding to maximal and minimal behavioral responses to FCF, respectively. Adult male Wistar rats (200-250 g) maintained on a 12-h light/12-h dark cycle (lights on at 7:00 h) were used. In the behavioral experiments the rats (N = 8 for each group) received FCF (3.5 mg/kg, ip) or saline at 9:00 or 21:00 h. Fifteen minutes after treatment the duration of activity (sniffing, rearing and locomotion) was recorded for 120 min. The basal motor activity was higher (28.6...
Palavras-chave: Fencamfamine; Chronopharmacology; Behavior; Dopamine uptake; Dopamine release.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500011

Association of apolipoprotein E polymorphism with plasma lipids and Alzheimer's disease in a Southern Brazilian population
Provedor de dados: 56
Autores: de-Andrade,F.M.; Larrandaburu,M.; Callegari-Jacques,S.M.; Gastaldo,G.; Hutz,M.H.
Apolipoprotein E (protein: apo E; gene: APOE) plays an important role in the multifactorial etiology of both Alzheimer's disease (AD) and lipid level concentrations. The polymerase chain reaction (PCR) was used to investigate the APOE gene polymorphism in 446 unrelated Caucasians, among them 23 AD patients, and 100 Afro-Brazilians living in Porto Alegre, Brazil. The frequencies of the APOE*2, APOE*3 and APOE*4 alleles were 0.075, 0.810 and 0.115 in Caucasians and 0.075, 0.700 and 0.225 in Afro-Brazilians, respectively (c2 = 8.72, P = 0.013). A highly significant association was observed between the APOE*4 allele and AD in this population-based sample. The APOE*4 frequency in AD patients (39%) was about four times higher than in the general Caucasian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; APOE; Lipoproteins; Apoprotein; Alzheimer's disease.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500007

Cyclin D1 expression in prostate carcinoma
Provedor de dados: 56
Autores: Pereira,R.A.; Ravinal,R.C.; Costa,R.S.; Lima,M.S.; Tucci,S.; Muglia,V.F.; Dos Reis,R.B.; Silva,G.E.B.
The purpose of this study was to investigate the relationship between cyclin D1 expression and clinicopathological parameters in patients with prostate carcinoma. We assessed cyclin D1 expression by conventional immunohistochemistry in 85 patients who underwent radical prostatectomy for prostate carcinoma and 10 normal prostate tissue samples retrieved from autopsies. We measured nuclear immunostaining in the entire tumor area and based the results on the percentage of positive tumor cells. The preoperative prostate-specific antigen (PSA) level was 8.68±5.16 ng/mL (mean±SD). Cyclin D1 staining was positive (cyclin D1 expression in &gt;5% of tumor cells) in 64 cases (75.4%) and negative (cyclin D1 expression in ≤5% of tumor cells) in 21 cases (including 15...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; Cyclin D1; Immunohistochemistry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600515

The leaves of green plants as well as a cyanobacterium, a red alga, and fungi contain insulin-like antigens
Provedor de dados: 56
Autores: Silva,L.B.; Santos,S.S.S.; Azevedo,C.R.; Cruz,M.A.L.; Venâncio,T.M.; Cavalcante,C.P.; Uchôa,A.F.; Astolfi Filho,S.; Oliveira,A.E.A.; Fernandes,K.V.S.; Xavier-Filho,J.
We report the detection of insulin-like antigens in a large range of species utilizing a modified ELISA plate assay and Western blotting. We tested the leaves or aerial parts of species of Rhodophyta (red alga), Bryophyta (mosses), Psilophyta (whisk ferns), Lycopodophyta (club mosses), Sphenopsida (horsetails), gymnosperms, and angiosperms, including monocots and dicots. We also studied species of fungi and a cyanobacterium, Spirulina maxima. The wide distribution of insulin-like antigens, which in some cases present the same electrophoretic mobility as bovine insulin, together with results recently published by us on the amino acid sequence of an insulin isolated from the seed coat of jack bean (Canavalia ensiformis) and from the developing fruits of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Insulin-like; Antigens; Evolution; Plants; Cyanobacteria; Algae; Fungi.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300004

Lack of evidence for the pathogenic role of iron and HFE gene mutations in Brazilian patients with nonalcoholic steatohepatitis
Provedor de dados: 56
Autores: Deguti,M.M.; Sipahi,A.M.; Gayotto,L.C.C.; Palácios,S.A.; Bittencourt,P.L.; Goldberg,A.C.; Laudanna,A.A.; Carrilho,F.J.; Cançado,E.L.R.
The hypothesis of the role of iron overload associated with HFE gene mutations in the pathogenesis of nonalcoholic steatohepatitis (NASH) has been raised in recent years. In the present study, biochemical and histopathological evidence of iron overload and HFE mutations was investigated in NASH patients. Thirty-two NASH patients, 19 females (59%), average 49.2 years, 72% Caucasians, 12% Mulattoes and 12% Asians, were submitted to serum aminotransferase and iron profile determinations. Liver biopsies were analyzed for necroinflammatory activity, architectural damage and iron deposition. In 31 of the patients, C282Y and H63D mutations were tested by PCR-RFLP. Alanine aminotransferase levels were increased in 30 patients, 2.42 ± 1.12 times the upper normal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatty liver; Nonalcoholic steatohepatitis; Iron overload; HFE gene; Alanine aminotransferase.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600009

Hemodynamic mechanisms of the attenuated blood pressure response to mental stress after a single bout of maximal dynamic exercise in healthy subjects
Provedor de dados: 56
Autores: Neves,F.J.; Carvalho,A.C.G.; Rocha,N.G.; Silva,B.M.; Sales,A.R.K.; Castro,R.R.T. de; Rocha,J.D.; Thomaz,T.G.; Nóbrega,A.C.L.
To determine the hemodynamic mechanisms responsible for the attenuated blood pressure response to mental stress after exercise, 26 healthy sedentary individuals (age 29 ± 8 years) underwent the Stroop color-word test before and 60 min after a bout of maximal dynamic exercise on a treadmill. A subgroup (N = 11) underwent a time-control experiment without exercise. Blood pressure was continuously and noninvasively recorded by infrared finger photoplethysmography. Stroke volume was derived from pressure signals, and cardiac output and peripheral vascular resistance were calculated. Perceived mental stress scores were comparable between mental stress tests both in the exercise (P = 0.96) and control (P = 0.24) experiments. After exercise, the blood pressure...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular system; Blood pressure; Exercise test; Stroop test.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700007

Selection of patients for myocardial perfusion scintigraphy based on fuzzy sets theory applied to clinical-epidemiological data and treadmill test results
Provedor de dados: 56
Autores: Duarte,P.S.; Mastrocolla,L.E.; Farsky,P.S.; Sampaio,C.R.E.P.S.; Tonelli,P.A.; Barros,L.C.; Ortega,N.R.; Pereira,J.C.R.
Coronary artery disease (CAD) is a worldwide leading cause of death. The standard method for evaluating critical partial occlusions is coronary arteriography, a catheterization technique which is invasive, time consuming, and costly. There are noninvasive approaches for the early detection of CAD. The basis for the noninvasive diagnosis of CAD has been laid in a sequential analysis of the risk factors, and the results of the treadmill test and myocardial perfusion scintigraphy (MPS). Many investigators have demonstrated that the diagnostic applications of MPS are appropriate for patients who have an intermediate likelihood of disease. Although this information is useful, it is only partially utilized in clinical practice due to the difficulty to properly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fuzzy model; Coronary disease; Scintigraphy; Myocardial perfusion.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100002

Effect of skilled and unskilled training on nerve regeneration and functional recovery
Provedor de dados: 56
Autores: Pagnussat,A.S.; Michaelsen,S.M.; Achaval,M.; Ilha,J.; Hermel,E.E.S.; Back,F.P.; Netto,C.A.
The most disabling aspect of human peripheral nerve injuries, the majority of which affect the upper limbs, is the loss of skilled hand movements. Activity-induced morphological and electrophysiological remodeling of the neuromuscular junction has been shown to influence nerve repair and functional recovery. In the current study, we determined the effects of two different treatments on the functional and morphological recovery after median and ulnar nerve injury. Adult Wistar male rats weighing 280 to 330 g at the time of surgery (N = 8-10 animals/group) were submitted to nerve crush and 1 week later began a 3-week course of motor rehabilitation involving either "skilled" (reaching for small food pellets) or "unskilled" (walking on a motorized treadmill)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peripheral nerve injury; Functional recovery; Median nerve; Ulnar nerve; Nerve morphometry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800010

A high-carbohydrate diet induces greater inflammation than a high-fat diet in mouse skeletal muscle
Provedor de dados: 56
Autores: Antunes,M.M.; Godoy,G.; de Almeida-Souza,C.B.; da Rocha,B.A.; da Silva-Santi,L.G.; Masi,L.N.; Carbonera,F.; Visentainer,J.V.; Curi,R.; Bazotte,R.B.
We previously reported that both the high-carbohydrate diet (HCD) and high-fat diet (HFD) given for two months promote lipid deposition and inflammation in the liver and brain of mice. The results obtained indicate a tissue-specific response to both diets. Herein, we compared the effects of HCD and HFD on fatty acid (FA) composition and inflammation in the gastrocnemius muscle. Male Swiss mice were fed with HCD or HFD for 1 or 2 months. Saturated FA (SFA), monounsaturated FA (MUFA), n-3 polyunsaturated FA (n-3 PUFA), and n-6 PUFA were quantified. The activities of stearoyl-CoA desaturase 1 (SCD-1), Δ-6 desaturase (D6D), elongase 6, and de novo lipogenesis (DNL) were estimated. As for indicators of the inflammatory tissue state, we measured myeloperoxidase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saturated fatty acids; Monounsaturated fatty acids; Polyunsaturated fatty acids; N-6/n-3 PUFA ratio; SFA/n-3 PUFA ratio.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300603

Noroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazil
Provedor de dados: 56
Autores: Gallimore,C.I.; Barreiros,M.A.B.; Brown,D.W.G.; Nascimento,J.P.; Leite,J.P.G.
Noroviruses (Norwalk-like viruses) are an important cause of gastroenteritis worldwide. They are the most common cause of outbreaks of gastroenteritis in the adult population and occur in nursing homes for the elderly, geriatric wards, medical wards, and in hotel and restaurant settings. Food-borne outbreaks have also occurred following consumption of contaminated oysters. This study describes the application of a reverse transcription-polymerase chain reaction (RT-PCR) assay using random primers (PdN6) and specific Ni and E3 primers, directed at a small region of the RNA-dependent RNA polymerase-coding region of the norovirus genome, and DNA sequencing for the detection and preliminary characterisation of noroviruses in outbreaks of gastroenteritis in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Noroviruses; Day care center; Gastroenteritis; Genotypes; Introduction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300005

Regulation of intercellular coupling in acute and chronic heart disease
Provedor de dados: 56
Autores: Saffitz,J.E.
Effective pump function of the heart depends on the precise control of spatial and temporal patterns of electrical activation. Accordingly, the distribution and function of gap junction channels are important determinants of the conduction properties of myocardium and undoubtedly play other roles in intercellular communication crucial to normal cardiac function. Recent advances have begun to elucidate mechanisms by which the heart regulates intercellular electrical coupling at gap junctions in response to stress or injury. Although responses to increased load or injury are generally adaptive in nature, remodeling of intercellular junctions under conditions of severe stress creates anatomic substrates conducive to the development of lethal ventricular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gap junctions; Cardiac hypertrophy; Heart failure; Arrhythmias.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400006

A carbohydrate-based mechanism of species recognition in sea urchin fertilization
Provedor de dados: 56
Autores: Mourão,P.A.S.
In the present review, we describe a systematic study of the sulfated polysaccharides from marine invertebrates, which led to the discovery of a carbohydrate-based mechanism of sperm-egg recognition during sea urchin fertilization. We have described unique polymers present in these organisms, especially sulfated fucose-rich compounds found in the egg jelly coat of sea urchins. The polysaccharides have simple, linear structures consisting of repeating units of oligosaccharides. They differ among the various species of sea urchins in specific patterns of sulfation and/or position of the glycosidic linkage within their repeating units. These polysaccharides show species specificity in inducing the acrosome reaction in sea urchin sperm, providing a clear-cut...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrosome reaction; Fertilization; Sulfated fucans; Glycosaminoglycans; Sulfated galactans; Species diversity; Sperm-egg recognition.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100002

Aberrant signaling in T-cell acute lymphoblastic leukemia: biological and therapeutic implications
Provedor de dados: 56
Autores: Cardoso,B.A.; Gírio,A.; Henriques,C.; Martins,L.R.; Santos,C.; Silva,A.; Barata,J.T.
T-cell acute lymphoblastic leukemia (T-ALL) is a biologically heterogeneous disease with respect to phenotype, gene expression profile and activation of particular intracellular signaling pathways. Despite very significant improvements, current therapeutic regimens still fail to cure a portion of the patients and frequently implicate the use of aggressive protocols with long-term side effects. In this review, we focused on how deregulation of critical signaling pathways, in particular Notch, PI3K/Akt, MAPK, Jak/STAT and TGF-ß, may contribute to T-ALL. Identifying the alterations that affect intracellular pathways that regulate cell cycle and apoptosis is essential to understanding the biology of this malignancy, to define more effective markers for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: T-cell acute lymphoblastic leukemia; Notch; PI3K/Akt; MAPK; Jak/STAT; TGF-ß.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500001

Selegiline increases heme oxygenase-1 expression and the cytotoxicity produced by dopamine treatment of neuroblastoma SK-N-SH cells
Provedor de dados: 56
Autores: Rieder,C.R.M.; Williams,A.C.; Ramsden,D.B.
Increased dopamine catabolism may be associated with oxidative stress and neuronal cell death in Parkinson's disease. The present study was carried out to examine the effect of dopamine on the expression of heme oxygenase-1 and -2 (HO-1 and HO-2) in human neuroblastomas (SK-N-SH cell line) and the effects of selegiline and antioxidants on this expression. Cells were kept with close control of pH and were incubated with varying concentrations of dopamine (0.1-100 µM) for 24 h. HO-1 and HO-2 cDNA probes were prepared by reverse transcription-polymerase chain reaction amplification. The mRNA expression of HO-1 and HO-2 was measured by Northern blot analysis. The levels of HO-1 mRNA increased after dopamine treatment, in a dose-dependent manner, in all cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Parkinson's disease; Dopamine; Selegiline.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700015

Prevalence of vascular-endothelial growth factor, matrix metalloproteinases and tissue inhibitors of metalloproteinases in primary breast cancer
Provedor de dados: 56
Autores: Jobim,F.C.; Xavier,N.L.; Uchoa,D.M.; Cruz,D.B.; Saciloto,M.; Chemello,N.; Schwartsmann,G.
Our objective was to determine the presence of vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2) and MMP-9 and specific tissue inhibitors of matrix metalloproteinase (TIMP-1 and TIMP-2) in tumor samples obtained from patients with primary breast cancer. We attempted to correlate these findings with the status of the sentinel lymph node (SLN) and clinical-pathological characteristics such as age, tumor size, histological type, histological grade, and vascular invasion. Tumor samples from 88 patients with primary breast cancer were analyzed. The immunoreactivity of VEGF, MMP-2, MMP-9, TIMP-1, and TIMP-2 in tumors was correlated with clinical and pathological features, as well as SLN status. Nonparametric, Mann-Whittney,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Vascular endothelial growth factor; Matrix metalloproteinase; Metalloproteinase tissue inhibitors; Sentinel lymph node.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000018

PPARγ induces growth inhibition and apoptosis through upregulation of insulin-like growth factor-binding protein-3 in gastric cancer cells
Provedor de dados: 56
Autores: Kim,S.Y.; Kim,M.S.; Lee,M.K.; Kim,J.S.; Yi,H.K.; Nam,S.Y.; Lee,D.Y.; Hwang,P.H.
Peroxisome proliferator activator receptor-gamma (PPARγ) is a ligand-activated transcriptional factor involved in the carcinogenesis of various cancers. Insulin-like growth factor-binding protein-3 (IGFBP-3) is a tumor suppressor gene that has anti-apoptotic activity. The purpose of this study was to investigate the anticancer mechanism of PPARγ with respect to IGFBP-3. PPARγ was overexpressed in SNU-668 gastric cancer cells using an adenovirus gene transfer system. The cells in which PPARγ was overexpressed exhibited growth inhibition, induction of apoptosis, and a significant increase in IGFBP-3 expression. We investigated the underlying molecular mechanisms of PPARγ in SNU-668 cells using an IGFBP-3 promoter/luciferase reporter system. Luciferase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PPARγ; IGFBP-3; Apoptosis; Gastric cancer.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300226

DNA extraction and quantification from touch and scrape preparations obtained from autopsy liver cells
Provedor de dados: 56
Autores: Ribeiro,C.N.M.; Peres,L.C.; Pina-Neto,J.M.
The objective of the present study was to develop a simplified low cost method for the collection and fixation of pediatric autopsy cells and to determine the quantitative and qualitative adequacy of extracted DNA. Touch and scrape preparations of pediatric liver cells were obtained from 15 cadavers at autopsy and fixed in 95% ethanol or 3:1 methanol:acetic acid. Material prepared by each fixation procedure was submitted to DNA extraction with the Wizard® genomic DNA purification kit for DNA quantification and five of the preparations were amplified by multiplex PCR (azoospermia factor genes). The amount of DNA extracted varied from 20 to 8,640 µg, with significant differences between fixation methods. Scrape preparation fixed in 95% ethanol provided...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autopsy; Congenital anomalies; DNA; Touch preparative; Scrape preparative; Polymerase chain reaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500002

The antioxidant action of Polypodium leucotomos extract and kojic acid: reactions with reactive oxygen species
Provedor de dados: 56
Autores: Gomes,A.J.; Lunardi,C.N.; Gonzalez,S.; Tedesco,A.C.
Two natural products Polypodium leucotomos extract (PL) and kojic acid (KA) were tested for their ability to scavenge reactive oxygen species (·OH, ·O2-, H2O2, ¹O2) in phosphate buffer. Hydroxyl radicals were generated by the Fenton reaction, and the rate constants of scavenging were 1.6 x 10(9) M-1 s-1 for KA and 1.0 x 10(9) M-1 s-1 for PL, similar to that of ethanol (1.4 x 10(9) M-1 s-1). With superoxide anions generated by the xanthine/hypoxanthine system, KA and PL (0.2-1.0 mg/ml) inhibited ·O2-dependent reduction of nitroblue tetrazolium by up to 30 and 31%, respectively. In the detection of ¹O2 by rose bengal irradiation, PL at 1.0 mg/ml quenched singlet oxygen by 43% relative to azide and KA by 36%. The present study demonstrates that PL showed an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polypodium leucotomos extract; Kojic acid; Antioxidant; Reactive oxygen species.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100018

Familial history of hypertension-induced impairment on heart rate variability was not observed in strength-trained subjects
Provedor de dados: 56
Autores: Santa-Rosa,F.A.; Shimojo,G.L.; Sartori,M.; Rocha,A.C.; Francica,J.V.; Paiva,J.; Irigoyen,M.C.; De Angelis,K.
Family history of hypertension is an important predictive factor for hypertension and is associated with hemodynamic and autonomic abnormalities. Previous studies reported that strength training might reduce arterial blood pressure (AP), as well as improve heart rate variability (HRV). However, the benefits of strength training in the offspring of hypertensive parents have not been fully evaluated. Here, we analyzed the impact of strength training on hemodynamics and autonomic parameters in offspring of hypertensive subjects. We performed a cross-sectional study with sedentary or physically active offspring of normotensives (S-ON and A-ON) or hypertensives (S-OH and A-OH). We recorded RR interval for analysis of HRV. AP was similar between groups....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Family history; Arterial blood pressure; Cardiac autonomic modulation; Sympathetic modulation; Strength training.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200605

Development of two potential diagnostic monoclonal antibodies against human cytomegalovirus glycoprotein B
Provedor de dados: 56
Autores: Jun,Fan; Minhuan,Li; Yadan,Ma; Yaping,Huang; Hanying,Liang; Jianhua,Hu; Hangping,Yao; Weihang,Ma.
Human cytomegalovirus glycoprotein B (gB) represents a target for diagnosis and treatment in view of the role it plays in virus entry and spread. Nevertheless, to our knowledge, rare detection of a gB antigen has been reported in transplant patients and limited information is available about diagnostic gB monoclonal antibodies (mAbs). Our aim was to develop gB mAbs with diagnostic potential. Hydrophilic gB peptides (ST: amino acids 27-40, SH: amino acids 81-94) of favorable immunogenicity were synthesized and used to immunize BALB/c mice. Two mAbs, named ZJU-FH6 and ZJU-FE6, were generated by the hybridoma technique and limited serial dilution and then characterized by indirect ELISA, Western blotting, immunoprecipitation, and immunohistochemical staining....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human cytomegalovirus; Glycoprotein B; Monoclonal antibody; Antigen detection; Transplantation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700004

Salvaged single-unit cord blood transplantation for 26 patients with hematologic malignancies not in remission
Provedor de dados: 56
Autores: Yao,W.; Zheng,C.C.; Liu,H.L.; Geng,L.Q.; Tang,B.L.; Tong,J.; Zhu,X.Y.; Song,K.D.; Qiang,P.; Sun,Z.M.
Treatments for patients with hematologic malignancies not in remission are limited, but a few clinical studies have investigated the effects of salvaged unrelated cord blood transplantation (CBT). We retrospectively studied 19 patients with acute leukemia, 5 with myelodysplastic syndrome (MDS with refractory anemia with excess blasts [RAEB]), and 2 with non-Hodgkin's lymphoma who received 1 CBT unit ≤2 loci human leukocyte antigen (HLA)-mismatched after undergoing myeloablative conditioning regimens between July 2005 and July 2014. All of them were in non-remission before transplantation. The infused total nucleated cell (TNC) dose was 4.07 (range 2.76-6.02)×107/kg and that of CD34+ stem cells was 2.08 (range 0.99-8.65)×105/kg. All patients were engrafted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Umbilical cord blood transplantation; Hematologic malignancies; Non-remission.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000002

Immunohistochemical detection of virus through its nuclear cytopathic effect in idiopathic interstitial pneumonia other than acute exacerbation
Provedor de dados: 56
Autores: Santos,G.C. dos; Parra,E.R.; Stegun,F.W.; Cirqueira,C.S.; Capelozzi,V.L.
Idiopathic interstitial pneumonias include complex diseases that have a strong interaction between genetic makeup and environmental factors. However, in many cases, no infectious agent can be demonstrated, and these clinical diseases rapidly progress to death. Theoretically, idiopathic interstitial pneumonias could be caused by the Epstein-Barr virus, cytomegalovirus, adenovirus, hepatitis C virus, respiratory syncytial virus, and herpesvirus, which may be present in such small amounts or such configuration that routine histopathological analysis or viral culture techniques cannot detect them. To test the hypothesis that immunohistochemistry provides more accurate results than the mere histological demonstration of viral inclusions, this method was applied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrosing interstitial pneumonia; Viral infections; Immunohistochemistry; Tissue microarray.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100985

Vascular adaptive responses to physical exercise and to stress are affected differently by nandrolone administration
Provedor de dados: 56
Autores: Bruder-Nascimento,T.; Cordellini,S.
Androgenic anabolic steroid, physical exercise and stress induce cardiovascular adaptations including increased endothelial function. The present study investigated the effects of these conditions alone and in combination on the vascular responses of male Wistar rats. Exercise was started at 8 weeks of life (60-min swimming sessions 5 days per week for 8 weeks, while carrying a 5% body-weight load). One group received nandrolone (5 mg/kg, twice per week for 8 weeks, im). Acute immobilization stress (2 h) was induced immediately before the experimental protocol. Curves for noradrenaline were obtained for thoracic aorta, with and without endothelium from sedentary and trained rats, submitted or not to stress, treated or not with nandrolone. None of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress; Physical exercise; Nandrolone; Vascular reactivity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400011

Social disability of Brazilian mood disorder patients
Provedor de dados: 56
Autores: Tucci,A.M.; Kerr-Corrêa,F.; Dias,R.S.
Mood disorders cause many social problems, often involving family relationships. Few studies are available in the literature comparing patients with bipolar, unipolar, dysthymic, and double depressive disorders concerning these aspects. In the present study, demographic and disease data were collected using a specifically prepared questionnaire. Social adjustment was assessed using the Disability Adjustment Scale and family relationships were evaluated using the Global Assessment of Relational Functioning Scale. One hundred patients under treatment for at least 6 months were evaluated at the Psychiatric Outpatient Clinic of the Botucatu School of Medicine, UNESP. Most patients were women (82%) more than 50 (49%) years old with at least two years of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mood disorders; Depression; Bipolar; Social adjustment; Unipolar.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100017

Epidemiological studies in the information and genomics era: experience of the Clinical Genome of Cancer Project in São Paulo, Brazil
Provedor de dados: 56
Autores: Wünsch-Filho,V.; Eluf-Neto,J.; Lotufo,P.A.; da Silva Jr.,W.A.; Zago,M.A.
Genomics is expanding the horizons of epidemiology, providing a new dimension for classical epidemiological studies and inspiring the development of large-scale multicenter studies with the statistical power necessary for the assessment of gene-gene and gene-environment interactions in cancer etiology and prognosis. This paper describes the methodology of the Clinical Genome of Cancer Project in São Paulo, Brazil (CGCP), which includes patients with nine types of tumors and controls. Three major epidemiological designs were used to reach specific objectives: cross-sectional studies to examine gene expression, case-control studies to evaluate etiological factors, and follow-up studies to analyze genetic profiles in prognosis. The clinical groups included...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multicenter studies; Large-scale studies; Molecular epidemiology; Data control quality; Cancer epidemiological studies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400016

Involvement of catecholaminergic medullary pathways in cardiovascular responses to acute changes in circulating volume
Provedor de dados: 56
Autores: Cravo,S.L.; Lopes,O.U; Pedrino,G.R.
Water deprivation and hypernatremia are major challenges for water and sodium homeostasis. Cellular integrity requires maintenance of water and sodium concentration within narrow limits. This regulation is obtained through engagement of multiple mechanisms and neural pathways that regulate the volume and composition of the extracellular fluid. The purpose of this short review is to summarize the literature on central neural mechanisms underlying cardiovascular, hormonal and autonomic responses to circulating volume changes, and some of the findings obtained in the last 12 years by our laboratory. We review data on neural pathways that start with afferents in the carotid body that project to medullary relays in the nucleus tractus solitarii and caudal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baroreceptors; A1 noradrenergic neurons; AV3V; Renal vascular conductance; Blood pressure; Hypertonic saline.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900008

Analysis of fracture healing in osteopenic bone caused by disuse: experimental study
Provedor de dados: 56
Autores: Paiva,A.G.; Yanagihara,G.R.; Macedo,A.P.; Ramos,J.; Issa,J.P.M.; Shimano,A.C.
Osteoporosis has become a serious global public health issue. Hence, osteoporotic fracture healing has been investigated in several previous studies because there is still controversy over the effect osteoporosis has on the healing process. The current study aimed to analyze two different periods of bone healing in normal and osteopenic rats. Sixty, 7-week-old female Wistar rats were randomly divided into four groups: unrestricted and immobilized for 2 weeks after osteotomy (OU2), suspended and immobilized for 2 weeks after osteotomy (OS2), unrestricted and immobilized for 6 weeks after osteotomy (OU6), and suspended and immobilized for 6 weeks after osteotomy (OS6). Osteotomy was performed in the middle third of the right tibia 21 days after tail...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoporosis; Osteotomy; Fracture healing; Immobilization; Rats.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300708

Interrelationship between adipocytes and fibroblasts during acute damage to the subcutaneous adipose tissue of rats: an ultrastructural study
Provedor de dados: 56
Ultrastructural phenotypic transitional features were noted between adult adipocytes and fibroblasts in the subcutaneous adipose tissue of the dorsal pad of normal adult Wistar rats of both sexes, weighing 180-260 g, after acute injury either by the implantation of small (1.8 x 1 x 0.4 cm) perforated plastic boxes or by local heat application. Soon after the inflicted damage, fat-containing cells presented variable shapes. Early after damage, some of these cells were round, adipocyte-like, with numerous and large cytoplasmic fat droplets. A few days later, fat-containing cells became elongated, with the fat droplets in their cytoplasm becoming smaller and less numerous. The cells also showed a prominent active rough endoplasmic reticulum and newly formed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipocytes; Adipocyte potential for differentiation; Adipose tissue damage.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500009

Growth inhibitory effect and Chk1-dependent signaling involved in G2/M arrest on human gastric cancer cells induced by diallyl disulfide
Provedor de dados: 56
Autores: Ling,H.; Wen,L.; Ji,X.X.; Tang,Y.L.; He,J.; Tan,H.; Xia,H.; Zhou,J.G.; Su,Q.
Diallyl disulfide (DADS) inhibits growth and induces cell cycle G2/M arrest in human gastric cancer MGC803 cells. In this study, 15 mg/L DADS exerted similar effects on growth and cell cycle arrest in human gastric cancer BGC823 cells. Due to the importance of cell cycle redistribution in DADS-mediated anti-carcinogenic effects, we investigated the role of checkpoint kinases (Chk1 and Chk2) during DADS-induced cell cycle arrest. We hypothesized that DADS could mediate G2/M phase arrest through either Chk1 or Chk2 signal transduction pathways. We demonstrated that DADS induced the accumulation of phosphorylated Chk1, but not of Chk2, and that DADS down-regulated Cdc25C and cyclin B1. The expression of mRNA and total protein for Chkl and Chk2 was unchanged....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diallyl disulfide; Cell cycle G2/M arrest; Checkpoint kinase-1; Gastric cancer.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300008

The RecX protein interacts with the RecA protein and modulates its activity in Herbaspirillum seropedicae
Provedor de dados: 56
Autores: Galvão,C.W.; Souza,E.M.; Etto,R.M.; Pedrosa,F.O.; Chubatsu,L.S.; Yates,M.G.; Schumacher,J.; Buck,M.; Steffens,M.B.R.
DNA repair is crucial to the survival of all organisms. The bacterial RecA protein is a central component in the SOS response and in recombinational and SOS DNA repairs. The RecX protein has been characterized as a negative modulator of RecA activity in many bacteria. The recA and recX genes of Herbaspirillum seropedicae constitute a single operon, and evidence suggests that RecX participates in SOS repair. In the present study, we show that the H. seropedicae RecX protein (RecX Hs) can interact with the H. seropedicaeRecA protein (RecA Hs) and that RecA Hs possesses ATP binding, ATP hydrolyzing and DNA strand exchange activities. RecX Hs inhibited 90% of the RecA Hs DNA strand exchange activity even when present in a 50-fold lower molar concentration than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RecA; RecX; Herbaspirillum seropedicae; SOS repair.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200004

The increased but non-predominant expression of Th17- and Th1-specific cytokines in Hashimoto’s thyroiditis but not in Graves’ disease
Provedor de dados: 56
Autores: Qin,Qiu; Liu,Ping; Liu,Lin; Wang,Rong; Yan,Ni; Yang,Jing; Wang,Xuan; Pandey,Madhu; Zhang,Jin-an.
Hashimoto’s thyroiditis (HT) is considered to be mediated mainly by Th1 cells, but it is not known whether Graves’ disease (GD) is associated with Th1 or Th2 predominance. Th17 cells, a novel subset of Th cells, play a crucial role in the pathogenesis of various autoimmune disorders. In the present study, the expression of IL-17A and IFN-γ was investigated in patients with HT or GD. mRNA expression of IL-17A and IFN-γ in peripheral blood mononuclear cells (PBMC) from 43 patients with autoimmune thyroid disease (AITD) and in thyroid tissues from 40 AITD patients were measured by real-time qRT-PCR. The protein expression of IL-17A and IL-23p19 was examined by immunohistochemistry in thyroid tissues from 28 AITD patients. The mRNA levels of IL-17A and IFN-γ...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Graves’ disease; Hashimoto’s thyroiditis; Interleukin-17; Interferon-γ.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200013

Where do we aspire to publish? A position paper on scientific communication in biochemistry and molecular biology
Provedor de dados: 56
Autores: Baptista,M.S.; Alves,M.J.M.; Arantes,G.M.; Armelin,H.A.; Augusto,O.; Baldini,R.L.; Basseres,D.S.; Bechara,E.J.H.; Bruni-Cardoso,A.; Chaimovich,H.; Colepicolo Neto,P.; Colli,W.; Cuccovia,I.M.; Da-Silva,A.M.; Di Mascio,P.; Farah,S.C.; Ferreira,C.; Forti,F.L.; Giordano,R.J.; Gomes,S.L.; Gueiros Filho,F.J.; Hoch,N.C.; Hotta,C.T.; Labriola,L.; Lameu,C.; Machini,M.T.; Malnic,B.; Marana,S.R.; Medeiros,M.H.G.; Meotti,F.C.; Miyamoto,S.; Oliveira,C.C.; Souza-Pinto,N.C.; Reis,E.M.; Ronsein,G.E.; Salinas,R.K.; Schechtman,D.; Schreier,S.; Setubal,J.C.; Sogayar,M.C.; Souza,G.M.; Terra,W.R.; Truzzi,D.R.; Ulrich,H.; Verjovski-Almeida,S.; Winck,F.V.; Zingales,B.; Kowaltowski,A.J.
The scientific publication landscape is changing quickly, with an enormous increase in options and models. Articles can be published in a complex variety of journals that differ in their presentation format (online-only or in-print), editorial organizations that maintain them (commercial and/or society-based), editorial handling (academic or professional editors), editorial board composition (academic or professional), payment options to cover editorial costs (open access or pay-to-read), indexation, visibility, branding, and other aspects. Additionally, online submissions of non-revised versions of manuscripts prior to seeking publication in a peer-reviewed journal (a practice known as pre-printing) are a growing trend in biological sciences. In this...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Scientific journals; Scientific editing; Pre-prints; Open access; Peer review.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900401

Cytokine accumulation in osteitis fibrosa of renal osteodystrophy
Provedor de dados: 56
Autores: Duarte,M.E.L.; Carvalho,E.F.; Cruz,E.A.S.; Lucena,S.B.G.; Andress,D.L.
Bone marrow fibrosis occurs in association with a number of pathological states. Despite the extensive fibrosis that sometimes characterizes renal osteodystrophy, little is known about the factors that contribute to marrow accumulation of fibrous tissue. Because circulating cytokines are elevated in uremia, possibly in response to elevated parathyroid hormone levels, we have examined bone biopsies from 21 patients with end-stage renal disease and secondary hyperparathyroidism. Bone sections were stained with antibodies to human interleukin-1alpha (IL-1alpha), IL-6, IL-11, tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-ß (TGF-ß) using an undecalcified plastic embedding method. Intense staining for IL-1alpha, IL-6, TNF-alpha and TGF-ß...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Osteitis fibrosa; Renal osteodystrophy; Chronic renal failure; Dialysis; Secondary hyperparathyroidism.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100004

Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts
Provedor de dados: 56
Autores: Zhang,L.; Ding,Y.; Rao,G.Z.; Miao,D.
The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P&lt;0.05), both in a concentration-dependent manner. Glucose stimulation had the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human periodontal ligament fibroblasts; Interleukin-10; Glucose; Osteoprotegerin; Receptor activator of nuclear factor-κB ligand.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400605

Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis
Provedor de dados: 56
Autores: Bedendo,J.; Pignatari,A.C.C.
Polymerase chain reaction (PCR) with JB1 or REP consensus oligonucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. Eleven ATCC strains, representative of 11 species of Enterococcus, were initially tested by JB1-PCR, revealing that Enterococcus malodoratus and Enterococcus hirae presented identical banding patterns. Eight Enterococcus faecium isolates from Stanford University and 12 from São Paulo Hospital were studied by JB1-PCR, REP-PCR 1/2R and PFGE. Among the isolates from Stanford University, 5 genotypes were defined by JB1-PCR, 7 by REP-PCR 1/2R and 4 by PFGE. Among the isolates from São Paulo Hospital, 9 genotypes were identified by JB1-PCR, 6 by REP-PCR and 5 by PFGE. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular typing; Enterococcus faecium; PCR-PFGE.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100002

Genetic engineering of baker's and wine yeasts using formaldehyde hyperresistance-mediating plasmids
Provedor de dados: 56
Autores: Schmidt,M.; Cömer,A.; Grey,M.; Brendel,M.
Yeast multi-copy vectors carrying the formaldehyde-resistance marker gene SFA have proved to be a valuable tool for research on industrially used strains of Saccharomyces cerevisiae. The genetics of these strains is often poorly understood, and for various reasons it is not possible to simply subject these strains to protocols of genetic engineering that have been established for laboratory strains of S. cerevisiae. We tested our vectors and protocols using 10 randomly picked baker's and wine yeasts all of which could be transformed by a simple protocol with vectors conferring hyperresistance to formaldehyde. The application of formaldehyde as a selecting agent also offers the advantage of its biodegradation to CO2 during fermentation, i.e., the selecting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Transformation; Hyperresistance to formaldehyde.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200004

Leptin and adiponectin in the female life course
Provedor de dados: 56
Autores: Lecke,S.B.; Morsch,D.M.; Spritzer,P.M.
Adipose tissue secretes a variety of adipokines, including leptin and adiponectin, which are involved in endocrine processes regulating glucose and fatty metabolism, energy expenditure, inflammatory response, immunity, cardiovascular function, and reproduction. The present article describes the fluctuations in circulating leptin and adiponectin as well as their patterns of secretion in women from birth to menopause. During pregnancy, leptin and adiponectin seem to act in an autocrine/paracrine fashion in the placenta and adipose tissue, playing a role in the maternal-fetal interface and contributing to glucose metabolism and fetal development. In newborns, adiponectin levels are two to three times higher than in adults. Full-term newborns have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Female; Adiposity; Leptin; Adiponectin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500001

Glycosphingolipid antigens from Leishmania (L.) amazonensis amastigotes: Binding of anti-glycosphingolipid monoclonal antibodies in vitro and in vivo
Provedor de dados: 56
Specific glycosphingolipid antigens of Leishmania (L.) amazonensis amastigotes reactive with the monoclonal antibodies (MoAbs) ST-3, ST-4 and ST-5 were isolated, and their structure was partially elucidated by negative ion fast atom bombardment mass spectrometry. The glycan moieties of five antigens presented linear sequences of hexoses and N-acetylhexosamines ranging from four to six sugar residues, and the ceramide moieties were found to be composed by a sphingosine d18:1 and fatty acids 24:1 or 16:0. Affinities of the three monoclonal antibodies to amastigote glycosphingolipid antigens were also analyzed by ELISA. MoAb ST-3 reacted equally well with all glycosphingolipid antigens tested, whereas ST-4 and ST-5 presented higher affinities to...
Palavras-chave: Glycosphingolipids; Monoclonal antibodies; Leishmania (L.) amazonensis; Fast atom bombardment mass spectrometry; Carbohydrate antigens.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300014

Biological X-ray irradiator characterization for use with small animals and cells
Provedor de dados: 56
Autores: Bruno,A. Colello; Mazaro,S.J.; Amaral,L.L.; Rego,E.M.; Oliveira,H.F.; Pavoni,J.F.
This study presents the characterization of an X-ray irradiator through dosimetric tests, which confirms the actual dose rate that small animals and cells will be exposed to during radiobiological experiments. We evaluated the linearity, consistency, repeatability, and dose distribution in the positions in which the animals or cells are placed during irradiation. In addition, we evaluated the performance of the X-ray tube (voltage and tube operating current), the radiometric survey (leakage radiation) and safety devices. The irradiator default setting was established as 160 kV and 25 mA. Tests showed that the dose rate was linear overtime (R2=1) and remained stable for long (constant) and short (repeatability) intervals between readings. The mean dose rate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: X-ray irradiator; Dosimetric characterization; Radiotherapy; Dosimetry; Radiochromic film.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300607

Relation of cervical length at 22-24 weeks of gestation to demographic characteristics and obstetric history
Provedor de dados: 56
Autores: Palma-Dias,R.S.; Fonseca,M.M.; Stein,N.R.; Schmidt,A.P.; Magalhães,J.A.
Preterm delivery is the main cause of neonatal death and ultrasonographic cervical assessment has been shown to be more accurate than digital examination in recognizing a short cervix. This is a cross-sectional study, involving 1131 women at 22-24 weeks of pregnancy, designed to determine the distribution of cervical length and to examine which variables of demographic characteristics and obstetric history increase the risk of a short cervix (15 mm or less). The distribution of maternal demographic and obstetric history characteristics among patients with cervical length £15 mm was analyzed and compared to the findings for the general population. Risk ratios (RR) between subgroups were generated from this comparison. Median cervical length was 37 mm and in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical length; Preterm delivery screening; Transvaginal sonography; Perinatal medicine.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500016

Developmental norms for the Gardner Steadiness Test and the Purdue Pegboard: a study with children of a metropolitan school in Brazil
Provedor de dados: 56
Autores: Brito,G.N.O.; Santos-Morales,T.R.
Norms for the Gardner Steadiness Test and the Purdue Pegboard were developed for the neuropsychological assessment of children in the metropolitan area of Rio de Janeiro. A computer-generated unbiased sample of 346 children with a mean age of 9.4 years (SD = 2.76), who were attending a large normal public school in this urban area, was the subject of this study. Two boys were removed from the study, one for refusing to participate and the other due to severe strabismus. Therefore, the final sample contained 344 children (173 boys and 171 girls). Sex and age of the child and hand preferred for writing, but not ethnic membership or social class, had significant effects on performance in the Gardner Steadiness Test and the Purdue Pegboard. Girls outperformed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuropsychology; Assessment instruments; Norms; Development; Cross-cultural differences; Manual differences; Sex differences; Brazil; Metropolitan children.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800011

Extracellular matrix molecules as targets for brown spider venom toxins
Provedor de dados: 56
Autores: Veiga,S.S.; Zanetti,V.C.; Braz,A.; Mangili,O.C.; Gremski,W.
Loxoscelism, the term used to describe lesions and clinical manifestations induced by brown spider's venom (Loxosceles genus), has attracted much attention over the last years. Brown spider bites have been reported to cause a local and acute inflammatory reaction that may evolve to dermonecrosis (a hallmark of envenomation) and hemorrhage at the bite site, besides systemic manifestations such as thrombocytopenia, disseminated intravascular coagulation, hemolysis, and renal failure. The molecular mechanisms by which Loxosceles venoms induce injury are currently under investigation. In this review, we focused on the latest reports describing the biological and physiopathological aspects of loxoscelism, with reference mainly to the proteases recently...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brown spider; Venom; Extracellular matrix; Proteolytic effect.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700002

Genotyping of group A rotavirus samples from Brazilian children by probe hybridization
Provedor de dados: 56
Autores: Cardoso,D.D.P.; Rácz,M.L.; Azevedo,M.S.P.; Martins,R.M.B.; Soares,C.M.A.
The G genotyping of 74 group A rotavirus samples was done by RNA-DNA hybridization (dot-blot) using oligonucleotide probes for the VP7 gene region of the human rotavirus serotypes/genotypes 1, 2, 3 and 4. Thirty-one samples could be genotyped by dot-blot showing the following results: G1 = 16, G4 = 6, G3 = 5, and G2 = 4. The data show circulation of genotypes G1-G4 and the predominance of G1. The knowledge of genotypes provides important information concerning rotavirus circulation in Central Brazil.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rotavirus; G genotyping; VP7; Oligonucleotides.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400005

Stretch-induced nerve injury: a proposed technique for the study of nerve regeneration and evaluation of the influence of gabapentin on this model
Provedor de dados: 56
Autores: Machado,J.A.; Ghizoni,M.F.; Bertelli,J.; Teske,Gabriel C.; Teske,Guilherme C.; Martins,D.F.; Mazzardo-Martins,L.; Cargnin-Ferreira,E.; Santos,A.R.S.; Piovezan,A.P.
The rat models currently employed for studies of nerve regeneration present distinct disadvantages. We propose a new technique of stretch-induced nerve injury, used here to evaluate the influence of gabapentin (GBP) on nerve regeneration. Male Wistar rats (300 g; n=36) underwent surgery and exposure of the median nerve in the right forelimbs, either with or without nerve injury. The technique was performed using distal and proximal clamps separated by a distance of 2 cm and a sliding distance of 3 mm. The nerve was compressed and stretched for 5 s until the bands of Fontana disappeared. The animals were evaluated in relation to functional, biochemical and histological parameters. Stretching of the median nerve led to complete loss of motor function up to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nerve regeneration; Median nerve; Motor activity; Rat model.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100929

Indomethacin attenuates mechanical allodynia during the organization but not the maintenance of the peripheral neuropathic pain induced by nervus ischiadicus chronic constriction injury
Provedor de dados: 56
Autores: Medeiros,P.; dos Santos,I.R.; Medeiros,A.C.; da Silva,J.A.; Ferreira,S.H.; de Freitas,R.L.; Coimbra,N.C.
The neurochemical mechanisms underlying neuropathic pain (NP) are related to peripheral and central sensitization caused by the release of inflammatory mediators in the peripheral damaged tissue and ectopic discharges from the injured nerve, leading to a hyperexcitable state of spinal dorsal horn neurons. The aim of this work was to clarify the role played by cyclooxygenase (COX) in the lesioned peripheral nerve in the development and maintenance of NP by evaluating at which moment the non-steroidal anti-inflammatory drug indomethacin, a non-selective COX inhibitor, attenuated mechanical allodynia after placing one loose ligature around the nervus ischiadicus, an adaptation of Bennett and Xie's model in rodents. NP was induced in male Wistar rats by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-steroidal anti-inflammatory drugs; Indomethacin; Chronic constriction injury of the nervus ischiadicus; Neuropathic pain; Mechanical allodynia; Von Frey test.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500606

Silibinin improves palmitate-induced insulin resistance in C2C12 myotubes by attenuating IRS-1/PI3K/Akt pathway inhibition
Provedor de dados: 56
Autores: Li,H.B.; Yang,Y.R.Y.; Mo,Z.J.; Ding,Y.; Jiang,W.J.
The present study investigated the effect of silibinin, the principal potential anti-inflammatory flavonoid contained in silymarin, a mixture of flavonolignans extracted from Silybum marianum seeds, on palmitate-induced insulin resistance in C2C12 myotubes and its potential molecular mechanisms. Silibinin prevented the decrease of insulin-stimulated 2-NBDG (2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose) uptake and the downregulation of glutamate transporter type 4 (GLUT4) translocation in C2C12 myotubes induced by palmitate. Meanwhile, silibinin suppressed the palmitate-induced decrease of insulin-stimulated Akt Ser473 phosphorylation, which was reversed by wortmannin, a specific inhibitor of phosphatidylinositol-3-kinase (PI3K). We also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silibinin; Insulin resistance; Palmitate; C2C12.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500440

Diabetic retinopathy is associated with early autonomic dysfunction assessed by exercise-related heart rate changes
Provedor de dados: 56
Autores: Kramer,C.K.; Leitão,C.B.; Azevedo,M.J.; Valiatti,F.B.; Rodrigues,T.C.; Canani,L.H.; Gross,J.L.
Diabetic retinopathy has been associated with cardiac autonomic dysfunction in both type 1 and type 2 diabetes mellitus (DM) patients. Heart rate (HR) changes during exercise testing indicate early alterations in autonomous tonus. The aim of the present study was to investigate the association of diabetic retinopathy with exercise-related HR changes. A cross-sectional study was performed on 72 type 2 and 40 type 1 DM patients. Autonomic dysfunction was assessed by exercise-related HR changes (Bruce protocol). The maximum HR increase, defined as the difference between the peak exercise rate and the resting rate at baseline, and HR recovery, defined as the reduction in HR from the peak exercise to the HR at 1, 2, and 4 min after the cessation of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes mellitus; Type 1 diabetes mellitus; Heart rate changes; Diabetic retinopathy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200011

Partial baroreceptor dysfunction and low plasma nitric oxide bioavailability as determinants of salt-sensitive hypertension: a reverse translational rat study
Provedor de dados: 56
Autores: Rodríguez-Pérez,A.S.; López-Rodríguez,J.F.; Calvo-Turrubiartes,M.Z.; Saavedra-Alanís,V.M.; Llamazares-Azuara,L.; Rodríguez-Martínez,M.
This study determined whether clinical salt-sensitive hypertension (cSSHT) results from the interaction between partial arterial baroreceptor impairment and a high-sodium (HNa) diet. In three series (S-I, S-II, S-III), mean arterial pressure (MAP) of conscious male Wistar ChR003 rats was measured once before (pdMAP) and twice after either sham (SHM) or bilateral aortic denervation (AD), following 7 days on a low-sodium (LNa) diet (LNaMAP) and then 21 days on a HNa diet (HNaMAP). The roles of plasma nitric oxide bioavailability (pNOB), renal medullary superoxide anion production (RMSAP), and mRNA expression of NAD(P)H oxidase and superoxide dismutase were also assessed. In SHM (n=11) and AD (n=15) groups of S-I, LNaMAP-pdMAP was 10.5±2.1 vs 23±2.1 mmHg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salt-sensitive hypertension; Aortic barodenervation; Nitric oxide bioavailability.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000868

Considerations about the present and future use of autoradiography
Provedor de dados: 56
Autores: Stumpf,W.E.
Tipo: Info:eu-repo/semantics/other
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200001

Hospital strain colonization by Staphylococcus epidermidis
Provedor de dados: 56
Autores: Blum-Menezes,D.; Bratfich,O.J.; Padoveze,M.C.; Moretti,M.L.
The skin and mucous membranes of healthy subjects are colonized by strains of Staphylococcus epidermidis showing a high diversity of genomic DNA polymorphisms. Prolonged hospitalization and the use of invasive procedures promote changes in the microbiota with subsequent colonization by hospital strains. We report here a patient with prolonged hospitalization due to chronic pancreatitis who was treated with multiple antibiotics, invasive procedures and abdominal surgery. We studied the dynamics of skin colonization by S. epidermidis leading to the development of catheter-related infections and compared the genotypic profile of clinical and microbiota strains by pulsed field gel electrophoresis. During hospitalization, the normal S. epidermidis skin...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Staphylococcus epidermidis; Microbiota; Biofilm; Central venous catheter.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300011

Regulation of gap junctions by protein phosphorylation
Provedor de dados: 56
Autores: Sáez,J.C.; Martínez,A.D.; Brañes,M.C.; González,H.E.
Gap junctions are constituted by intercellular channels and provide a pathway for transfer of ions and small molecules between adjacent cells of most tissues. The degree of intercellular coupling mediated by gap junctions depends on the number of gap junction channels and their activity may be a function of the state of phosphorylation of connexins, the structural subunit of gap junction channels. Protein phosphorylation has been proposed to control intercellular gap junctional communication at several steps from gene expression to protein degradation, including translational and post-translational modification of connexins (i.e., phosphorylation of the assembled channel acting as a gating mechanism) and assembly into and removal from the plasma membrane....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gap junctions; Connexins; Protein phosphorylation; Sites of phosphorylation; Protein kinases; Cell-to-cell communication.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500001

Involvement of hippocampal NMDA receptors in retention of shuttle avoidance conditioning in rats
Provedor de dados: 56
Autores: Roesler,R.; Kuyven,C.R.; Kruel,A.V.S.; Quevedo,J.; Ferreira,M.B.C.
The purpose of this research was to evaluate the role of hippocampal N-methyl-D-aspartate (NMDA) receptors in acquisition and consolidation of memory during shuttle avoidance conditioning in rats. Adult male Wistar rats were surgically implanted with cannulae aimed at the CA1 area of the dorsal hippocampus. After recovery from surgery, animals were trained and tested in a shuttle avoidance apparatus (30 trials, 0.5-mA footshock, 24-h training-test interval). Immediately before or immediately after training, animals received a bilateral intrahippocampal 0.5-µl infusion containing 5.0 µg of the NMDA competitive receptor antagonist aminophosphonopentanoic acid (AP5) or vehicle (phosphate-buffered saline, pH 7.4). Infusion duration was 2 min per side....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: NMDA receptor; Hippocampus; Memory; Conditioning; Fear.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200014

Effect of exercise training and carvedilol treatment on cardiac function and structure in mice with sympathetic hyperactivity-induced heart failure
Provedor de dados: 56
Autores: Medeiro,A.; Vanzelli,A.S.; Rosa,K.T.; Irigoyen,M.C.; Brum,P.C.
The present investigation was undertaken to study the effect of β-blockers and exercise training on cardiac structure and function, respectively, as well as overall functional capacity in a genetic model of sympathetic hyperactivity-induced heart failure in mice (α2A/α2CArKO). α2A/α2CArKO and their wild-type controls were studied for 2 months, from 3 to 5 months of age. Mice were randomly assigned to control (N = 45), carvedilol-treated (N = 29) or exercise-trained (N = 33) groups. Eight weeks of carvedilol treatment (38 mg/kg per day by gavage) or exercise training (swimming sessions of 60 min, 5 days/week) were performed. Exercise capacity was estimated using a graded treadmill protocol and HR was measured by tail cuff. Fractional shortening was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Exercise training; Carvedilol treatment; Ventricular function; Cardiac remodeling; Α2A/α2CArKO mice.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900012

Whole-body vibration decreases the proliferativeb response of TCD4+ cells in elderly individuals with knee osteoarthritis
Provedor de dados: 56
Autores: Tossige-Gomes,R.; Avelar,N.C.P.; Simão,A.P.; Neves,C.D.C.; Brito-Melo,G.E.A.; Coimbra,C.C.; Rocha-Vieira,E.; Lacerda,A.C.R.
The aim of this study was to investigate the effect of adding whole-body vibration (WBV; frequency = 35 to 40 Hz; amplitude = 4 mm) to squat training on the T-cell proliferative response of elderly patients with osteoarthritis (OA) of the knee. This study was a randomized controlled trial in which the selected variables were assessed before and after 12 weeks of training. Twenty-six subjects (72 ± 5 years of age) were divided into three groups: 1) squat training with WBV (WBV, N = 8); 2) squat training without WBV (N = 10), and 3) a control group (N = 8). Women who were ≥60 years of age and had been diagnosed with OA in at least one knee were eligible. The intervention consisted of 12 uninterrupted weeks of squatting exercise training performed 3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vibratory platform; Lymphocyte; Proliferation; Elderly; Osteoarthritis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200022

EEG spike source localization before and after surgery for temporal lobe epilepsy: a BOLD EEG-fMRI and independent component analysis study
Provedor de dados: 56
Autores: Sercheli,M.S.; Bilevicius,E.; Alessio,A.; Ozelo,H.; Pereira,F.R.S.; Rondina,J.M.; Cendes,F.; Covolan,R.J.M.
Simultaneous measurements of EEG-functional magnetic resonance imaging (fMRI) combine the high temporal resolution of EEG with the distinctive spatial resolution of fMRI. The purpose of this EEG-fMRI study was to search for hemodynamic responses (blood oxygen level-dependent - BOLD responses) associated with interictal activity in a case of right mesial temporal lobe epilepsy before and after a successful selective amygdalohippocampectomy. Therefore, the study found the epileptogenic source by this noninvasive imaging technique and compared the results after removing the atrophied hippocampus. Additionally, the present study investigated the effectiveness of two different ways of localizing epileptiform spike sources, i.e., BOLD contrast and independent...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Interictal epileptiform spike; Epilepsy surgery; EEG-fMRI; Temporal lobe epilepsy; ICA; Dipole model.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600017

Correction
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/article
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700014

Trans-sialidase delivered as a naked DNA vaccine elicits an immunological response similar to a Trypanosoma cruzi infection
Provedor de dados: 56
Autores: Costa,F.; Pereira-Chioccola,V.L.; Ribeirão,M.; Schenkman,S.; Rodrigues,M.M.
Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease, does not synthesize sialic acid, but expresses a trans-sialidase (TS) that catalyzes the transfer of sialic acid from host glycoconjugates to the parasite surface. Here, we review studies that characterize the immune response to the catalytic domain of the enzyme in humans during Chagas' disease or in mice following immunization with the TS gene. In both cases, there are antibodies that strongly inhibit the enzymatic activity and generation of interferon-&lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt;-producing T cells.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA vaccination; Trans-sialidase; Chagas' disease.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200013

Occult hepatitis B virus infection in liver transplant patients in a Brazilian referral center
Provedor de dados: 56
Autores: Ferrari,T.C.A.; Xavier,M.A.P.; Vidigal,P.V.T.; Amaral,N.S.; Diniz,P.A.; Resende,A.P.; Miranda,D.M.; Faria,A.C.; Lima,A.S.; Faria,L.C.
Estimates of occult hepatitis B virus (HBV) infection prevalence varies among different studies depending on the prevalence of HBV infection in the study population and on the sensitivity of the assay used to detect HBV DNA. We investigated the prevalence of occult HBV infection in cirrhotic patients undergoing liver transplantation in a Brazilian referral center. Frozen liver samples from 68 adults were analyzed using a nested polymerase chain reaction assay for HBV DNA. The specificity of the amplified HBV sequences was confirmed by direct sequencing of the amplicons. The patient population comprised 49 (72.1%) males and 19 (27.9%) females with a median age of 53 years (range=18-67 years). Occult HBV infection was diagnosed in three (4.4%) patients. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Occult hepatitis B virus infection; Hepatitis B virus; HBV DNA; Liver transplantation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100990

Pitfalls in the assessment of murine atherosclerosis
Provedor de dados: 56
Autores: Catanozi,S.; Rocha,J.C.; Passarelli,M.; Chiquito,F.C.; Quintão,E.C.R.; Nakandakare,E.R.
This review provides examples of the fact that different procedures for the measurement of atherosclerosis in mice may lead to interpretation of the extent of atherosclerosis having markedly different biological and clinical significance for humans: 1) aortic cholesterol measurement is highly sensitive for the detection of early and advanced atherosclerosis lesions, but misses the identification of the location and complexity of these lesions that are so critical for humans; 2) the histological analysis of the aortic root lesions in simvastatin-treated and control mice reveals similar lesion morphology in spite of the remarkable simvastatin-induced reduction of the aortic cholesteryl ester content; 3) in histological analyses, chemical fixation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Animal models; Disease models; Aorta; Pathology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600001

Opioidergic orofacial antinociception induced by electroacupuncture at acupoint St36
Provedor de dados: 56
Autores: Almeida,R.T.; Perez,A.C.; Francischi,J.N.; Castro,M.S.; Duarte,I.D.G.
The participation of opioids in the antinociceptive effect of electroacupuncture was evaluated in terms of nociception produced by thermal stimuli applied to the face of male Wistar rats, weighing 180-230 g. Electrical stimulation (bipolar and asymmetric square wave with 0.5 mA intensity for 20 min) of acupoint St36, located in the anterior tibial muscle 10 mm distal to the knee joint, induced antinociception in the present model, which was maintained for 150 min. Acupoint LI4, located in the junction of the first and second metacarpal bones, did not achieve antinociception at any frequency studied (5 Hz: 1.7 ± 0.1; 30 Hz: 1.8 ± 0.1; 100 Hz: 1.7 ± 0.1 vs 1.4 ± 0.2). The antinociception obtained by stimulation of acupoint St36 was only achieved when high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acupuncture; St36 acupoint; Opioid; Orofacial antinociception.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700012

Role of high mobility group box-1 and protection of growth hormone and somatostatin in severe acute pancreatitis
Provedor de dados: 56
Autores: Wang,Y.F.; Wu,M.; Ma,B.J.; Cai,D.A.; Yin,B.B.
In this study, we investigated the potential role of high-mobility group box 1 (HMGB1) in severe acute pancreatitis (SAP) and the effects of growth hormone (G) and somatostatin (S) in SAP rats. The rats were randomly divided into 6 groups of 20 each: sham-operated, SAP, SAP+saline, SAP+G, SAP+S and SAP+G+S. Ileum and pancreas tissues of rats in each group were evaluated histologically. HMGB1 mRNA expression was measured by reverse transcription-PCR. Levels of circulating TNF-α, IL-1, IL-6, and endotoxin were also measured. In the SAP group, interstitial congestion and edema, inflammatory cell infiltration, and interstitial hemorrhage occurred in ileum and pancreas tissues. The levels of HMGB1, TNF-α, IL-1, IL-6 and endotoxin were significantly up-regulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Severe acute pancreatitis; High-mobility group box 1; Growth hormone; Somatostatin; Inflammatory response.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201075

IGF2, LEPR, POMC, PPARG, and PPARGC1 gene variants are associated with obesity-related risk phenotypes in Brazilian children and adolescents
Provedor de dados: 56
Autores: Queiroz,E.M.; Cândido,A.P.C.; Castro,I.M.; Bastos,A.Q.A.; Machado-Coelho,G.L.L.; Freitas,R.N.
Association studies of genetic variants and obesity and/or obesity-related risk factors have yielded contradictory results. The aim of the present study was to determine the possible association of five single-nucleotide polymorphisms (SNPs) located in the IGF2, LEPR, POMC, PPARG, and PPARGC1 genes with obesity or obesity-related risk phenotypes. This case-control study assessed overweight (n=192) and normal-weight (n=211) children and adolescents. The SNPs were analyzed using minisequencing assays, and variables and genotype distributions between the groups were compared using one-way analysis of variance and Pearson's chi-square or Fisher's exact tests. Logistic regression analysis adjusted for age and gender was used to calculate the odds ratios (ORs)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Association study; Obesity; Genetic polymorphisms; Brazilian population.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700595

Evaluation by blue native polyacrylamide electrophoresis colorimetric staining of the effects of physical exercise on the activities of mitochondrial complexes in rat muscle
Provedor de dados: 56
Autores: Molnar,A.M.; Alves,A.A.; Pereira-da-Silva,L.; Macedo,D.V.; Dabbeni-Sala,F.
Blue native polyacrylamide electrophoresis (BN-PAGE) is a technique developed for the analysis of membrane complexes. Combined with histochemical staining, it permits the analysis and quantification of the activities of mitochondrial oxidative phosphorylation enzymes using whole muscle homogenates, without the need to isolate muscle mitochondria. Mitochondrial complex activities were measured by emerging gels in a solution containing all specific substrates for NADH dehydrogenase and cytochrome c oxidase enzymes (complexes I and IV, respectively) and the colored bands obtained were measured by optique densitometry. The objective of the present study was the application of BN-PAGE colorimetric staining for enzymatic characterization of mitochondrial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BN-PAGE; Colorimetric staining; Exhaustive physical exercise; Mitochondrial complexes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700001

Ischemic post-conditioning attenuates the intestinal injury induced by limb ischemia/reperfusion in rats
Provedor de dados: 56
Autores: Leng,Y.F.; Zhang,Y.; Zhang,Y.; Xue,X.; Wang,T.; Kang,Y.Q.
The purpose of this study was to investigate the protective effects of ischemic post-conditioning on damage to the barrier function of the small intestine caused by limb ischemia-reperfusion injury. Male Wistar rats were randomly divided into 3 groups (N = 36 each): sham operated (group S), lower limb ischemia-reperfusion (group LIR), and post-conditioning (group PC). Each group was divided into subgroups (N = 6) according to reperfusion time: immediate (0 h; T1), 1 h (T2), 3 h (T3), 6 h (T4), 12 h (T5), and 24 h (T6). In the PC group, 3 cycles of reperfusion followed by ischemia (each lasting 30 s) were applied immediately. At all reperfusion times (T1-T6), diamine oxidase (DAO), superoxide dismutase (SOD), and myeloperoxidase (MPO) activity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Post-conditioning; Limb ischemia-reperfusion injury; Small intestine.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500005

The effect of down-regulation of Smad3 by RNAi on hepatic stellate cells and a carbon tetrachloride-induced rat model of hepatic fibrosis
Provedor de dados: 56
Autores: Wang,Z.R.; Wang,J.H.; Hu,C.L.; Cao,W.G.; Shen,X.J.; Wu,M.Y.; Shen,L.; Wu,S.L.
Searching for effective Smad3 gene-based gene therapies for hepatic fibrosis, we constructed siRNA expression plasmids targeting the rat Smad3 gene and then delivered these plasmids into hepatic stellate cells (HSCs). The effect of siRNAs on the mRNA levels of Smad2, Smad3, Smad4, and collagens I-α1, III-α1 and IV-α1 (Colα1, Col3α1, Col4α1, respectively) was determined by RT-PCR. Eighty adult male Sprague-Dawley rats were randomly divided into three groups. Twice a week for 8 weeks, the untreated hepatic fibrosis model (N = 30) and the treated group (N = 20) were injected subcutaneously with 40% (v/v) carbon tetrachloride (CCl4)-olive oil (3 mL/kg), and the normal control group (N = 30) was injected with olive oil (3 mL/kg). In the 4th week, the treated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smad3; SiRNA; Hepatic fibrosis; Carbon tetrachloride.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200002

Measurement of the ultrasonic properties of human coronary arteries in vitro with a 50-MHz acoustic microscope
Provedor de dados: 56
Autores: Machado,J.C.; Foster,F.S.; Gotlieb,A.I.
Ultrasonic attenuation coefficient, wave propagation speed and integrated backscatter coefficient (IBC) of human coronary arteries were measured in vitro over the -6 dB frequency bandwidth (36 to 67 MHz) of a focused ultrasound transducer (50 MHz, focal distance 5.7 mm, f/number 1.7). Corrections were made for diffraction effects. Normal and diseased coronary artery sub-samples (N = 38) were obtained from 10 individuals at autopsy. The measured mean ± SD of the wave speed (average over the entire vessel wall thickness) was 1581.04 ± 53.88 m/s. At 50 MHz, the average attenuation coefficient was 4.99 ± 1.33 dB/mm with a frequency dependence term of 1.55 ± 0.18 determined over the 36- to 67-MHz frequency range. The IBC values were: 17.42 ± 13.02 (sr.m)-1 for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acoustic microscopy; Coronary artery wall; Ultrasound tissue characterization; Integrated backscatter coefficient.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800006

Loss to follow-up in anti-HCV-positive patients in a Brazilian regional outpatient clinic
Provedor de dados: 56
Autores: Mendes,L.C.; Ralla,S.M.; Vigani,A.G.
Loss to follow-up (LF), which refers to patients who started care but voluntary stopped it, is a problem for patients with chronic disease. We aimed to estimate the rate of LF among patients seropositive for hepatitis C virus (HCV) and identify possible demographic and lifestyle risk factors associated with LF. From January 2009 through December 2012, 1010 anti-HCV-positive patients were included in the study. Among participants, 223 (22.1%) met the case definition for LF (more than 1-year elapsed since the last clinical appointment). Among 787 patients who remained in follow-up, 372 (47.2%) were discharged after undetectable HCV RNA, 88 (11.1%) were transferred (and remained on regular follow-up at the destination), and 25 (3.1%) died. According to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; Follow-up management; Adherence.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000702

Effects of Rab7 gene up-regulation on renal fibrosis induced by unilateral ureteral obstruction
Provedor de dados: 56
Autores: Xu,Qing; Liu,Lei; Yang,Yiqiong; Wang,Zhi; Cai,Yingying; Hong,Tingting; Chen,Pingsheng.
Rab7, an important member of the Rab family, is closely related to autophagy, endocytosis, apoptosis, and tumor suppression but few studies have described its association with renal fibrosis. In the early stage, our group studied the effects of Rab7 on production and degradation of extracellular matrix in hypoxic renal tubular epithelial cells. Because cell culture in vitro is different from the environment in vivo, it is urgent to understand the effects in vivo. In our current study, we established a renal fibrosis model in Rab7-knock-in mice (prepared by CRISPR/Cas9 technology) and wild type (WT) C57BL/6 mice using unilateral ureteral obstruction (UUO). Seven and 14 days after UUO, the expression of the Rab7 protein in WT mice, as well as the autophagic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rab7; Autophagy; Renal fibrosis; Crispr/cas9; Endocytosis; Lipophagy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400603

Dimensionality of the premenstrual syndrome: confirmatory factor analysis of premenstrual dysphoric symptoms among college students
Provedor de dados: 56
Autores: Wang,Y.-P.; Teng,C.-T.; Vieira Filho,A.H.G.; Gorenstein,C.; Andrade,L.H.
Premenstrual syndrome and premenstrual dysphoric disorder (PMDD) seem to form a severity continuum with no clear-cut boundary. However, since the American Psychiatric Association proposed the research criteria for PMDD in 1994, there has been no agreement about the symptomatic constellation that constitutes this syndrome. The objective of the present study was to establish the core latent structure of PMDD symptoms in a non-clinical sample. Data concerning PMDD symptoms were obtained from 632 regularly menstruating college students (mean age 24.4 years, SD 5.9, range 17 to 49). For the first random half (N = 316), we performed principal component analysis (PCA) and for the remaining half (N = 316), we tested three theory-derived competing models of PMDD by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Premenstrual dysphoric disorder; Premenstrual syndrome; Dysphoria; Factor analysis; Psychometric properties.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500006

MicroRNA-4290 suppresses PDK1-mediated glycolysis to enhance the sensitivity of gastric cancer cell to cisplatin
Provedor de dados: 56
Autores: Qian,Yan; Wu,Xu; Wang,Haixiao; Hou,Guowei; Han,Xiao; Song,Wei.
The development of chemotherapy resistance significantly impairs the efficiency of chemotherapy, but the underlying mechanisms of chemotherapy resistance in gastric cancer (GC) are complicated and still need to be further explored. Here, we aimed to reveal the effects of miR-4290/PDK1 (pyruvate dehydrogenase kinase 1) axis on chemotherapy resistance of GC in vitro. The expression patterns of miR-4290 in GC tissues and cell lines were determined by real-time quantitative PCR. Kaplan-Meier was used to assess the relationship between miR-4290 expression levels and patients' overall survival. CCK-8 and flow cytometry technologies were applied to detect cell proliferation and apoptosis. The luciferase gene reporter assay was used to evaluate the interaction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-4290; PDK1; Cisplatin; Chemoresistance; Gastric cancer.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500604

Analysis of acylcarnitine profiles in umbilical cord blood and during the early neonatal period by electrospray ionization tandem mass spectrometry
Provedor de dados: 56
Autores: Vieira Neto,E.; Fonseca,A.A.; Almeida,R.F.; Figueiredo,M.P.; Porto,M.A.S.; Ribeiro,M.G.
Acylcarnitine profiling by electrospray ionization tandem mass spectrometry (ESI-MS/MS) is a potent tool for the diagnosis and screening of fatty acid oxidation and organic acid disorders. Few studies have analyzed free carnitine and acylcarnitines in dried blood spots (DBS) of umbilical cord blood (CB) and the postnatal changes in the concentrations of these analytes. We have investigated these metabolites in healthy exclusively breastfed neonates and examined possible effects of birth weight and gestational age. DBS of CB were collected from 162 adequate for gestational age neonates. Paired DBS of heel-prick blood were collected 4-8 days after birth from 106 of these neonates, the majority exclusively breastfed. Methanol extracts of DBS with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acylcarnitine; Carnitine Cord blood Electrospray ionization mass spectrometry Newborn infants Reference values.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600011

A Portuguese version of the Christo Inventory for Substance-Misuse Services: a simple outcome evaluation tool
Provedor de dados: 56
Autores: Christo,G.; Silva,V.A.
The Christo Inventory for Substance-Misuse Services (CISS) is a single page outcome evaluation tool completed by drug alcohol service workers either on the basis of direct client interviews or of personal experience of their client supplemented by existing assessment notes. It was developed to assist substance misuse services to empirically demonstrate the effectiveness of their treatments to their respective funding bodies. Its 0 to 20 unidimensional scale consists of 10 items reflecting clients' problems with social functioning, general health, sexual/injecting risk behavior, psychological functioning, occupation, criminal involvement, drug/alcohol use, ongoing support, compliance, and working relationships. Good reliability and validity has already been...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Substance misuse; Alcohol; Treatment outcome; Evaluation; Clinical audit.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000001

IV Miguel R. Covian Symposium: new challenges for physiologists
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/other
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100001

Estrogen and progesterone receptors in human papilloma virus-related cervical neoplasia
Provedor de dados: 56
Autores: Coelho,F.R.G.; Prado,J.C.M.; Pereira Sobrinho,J.S.; Hamada,G.; Landman,G.; Pinto,C.A.; Nonogaki,S.; Villa,L.L.
Estrogen (ER) and progesterone (PR) receptors in the normal uterine cervix, cervical intraepithelial neoplasia and invasive carcinoma were studied in consecutive samples from Hospital do Câncer, São Paulo, between 1996 and 1997. Tissue was collected by removing a fragment of the tumoral area using a 5-mm diameter biopsy punch, followed by removal of a macroscopically normal area as close as possible from the tumor. Histopathological confirmation was obtained for all specimens analyzed. A total of 24 normal tissues, 17 cases of cervical intraepithelial neoplasia and 7 of invasive carcinomas were studied. The ER/PR ratio was determined by immunohistochemistry using monoclonal antibodies specific for each receptor. Adjacent tissue slides were submitted to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Estrogen receptor; Progesterone receptor; Cervical cancer; Cervical intraepithelial neoplasia; Human papillomavirus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100012

Comparison of I-FISH and G-banding for the detection of chromosomal abnormalities during the evolution of myelodysplastic syndrome
Provedor de dados: 56
Autores: Pinheiro,R.F.; Chauffaille,M.L.L.F.
Myelodysplastic syndrome (MDS) patients with a normal karyotype constitute a heterogeneous group from a biological standpoint and their outcome is often unpredictable. Interphase fluorescence in situ hybridization (I-FISH) studies could increase the rate of detection of abnormalities, but previous reports in the literature have been contradictory. We performed I-FISH and conventional karyotyping (G-banding) on 50 MDS patients at diagnosis, after 6 and 12 months or at any time if a transformation to acute myeloid leukemia (AML) was detected. Applying a probe-panel targeting the centromere of chromosomes 7 and 8, 5q31, 5p15.2 and 7q31, we observed one case with 5q deletion not identified by G-banding. I-FISH at 6 and 12 months confirmed the karyotype...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myelodysplastic syndrome; FISH; G-banding.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100018

Attenuation of phosphamidon-induced oxidative stress and immune dysfunction in rats treated with N-acetylcysteine
Provedor de dados: 56
Autores: Suke,S.G.; Ahmed,R.S.; Pathak,R.; Tripathi,A.K.; Banerjee,B.D.
The effect of N-acetylcysteine, a thiolic antioxidant, on attenuation of phosphamidon-induced oxidative stress and immune dysfunction was evaluated in adult male Wistar rats weighing 200-250 g. Rats were divided into four groups, 8 animals/group, and treated with phosphamidon, N-acetylcysteine or the combination of both for 28 days. Oral administration of phosphamidon (1.74 mg/kg), an organophosphate insecticide, increased serum malondialdehyde (3.83 ± 0.18 vs 2.91 ± 0.24 nmol/mL; P &lt; 0.05) and decreased erythrocyte superoxide dismutase (567.8 ± 24.36 vs 749.16 ± 102.61 U/gHb; P &lt; 0.05), catalase activity (1.86 ± 0.18 vs 2.43 ± 0.08 U/gHb; P &lt; 0.05) and whole blood glutathione levels (1.25 ± 0.21 vs 2.28 ± 0.08 mg/gHb; P &lt; 0.05) showing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Antioxidant; Organophosphate; Oxidative stress; Immunotoxicity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900004

Early remodeling of rat cardiac muscle induced by swimming training
Provedor de dados: 56
Autores: Verzola,R.M.M.; Mesquita,R.A.; Peviani,S.; Ramos,O.H.P.; Moriscot,A.S.; Perez,S.E.A.; Selistre-de-Araújo,H.S.
The aim of the present investigation was to study the effect of acute swimming training with an anaerobic component on matrix metallopeptidase (MMP) activity and myosin heavy chain gene expression in the rat myocardium. Animals (male Wistar rats, weighing approximately 180 g) were trained for 6 h/day in 3 sessions of 2 h each for 1 to 5 consecutive days (N = 5 rats per group). Rats swam in basins 47 cm in diameter and 60 cm deep filled with water at 33 to 35ºC. After the training period a significant increase (P &lt; 0.05) was observed in the heart weight normalized to body weight by about 22 and 35% in the groups that trained for 96 and 120 h, respectively. Blood lactate levels were significantly increased (P &lt; 0.05) in all groups after all training...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myosin heavy chain expression; Training; Real time PCR; Rat myocardium; Muscle hypertrophy.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500009

Statistical analysis of event-related potential elicited by verb-complement merge in Brazilian Portuguese
Provedor de dados: 56
Autores: Cagy,M.; Infantosi,A.F.C.; França,A.I.; Lemle,M.
An interesting fact about language cognition is that stimulation involving incongruence in the merge operation between verb and complement has often been related to a negative event-related potential (ERP) of augmented amplitude and latency of ca. 400 ms - the N400. Using an automatic ERP latency and amplitude estimator to facilitate the recognition of waves with a low signal-to-noise ratio, the objective of the present study was to study the N400 statistically in 24 volunteers. Stimulation consisted of 80 experimental sentences (40 congruous and 40 incongruous), generated in Brazilian Portuguese, involving two distinct local verb-argument combinations (nominal object and pronominal object series). For each volunteer, the EEG was simultaneously acquired at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Event-related brain potentials; Verb-complement merges; Averaging; N400 identification.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100010

Influence of schooling and age on cognitive performance in healthy older adults
Provedor de dados: 56
Autores: Bento-Torres,N.V.O.; Bento-Torres,J.; Tomás,A.M.; Costa,V.O.; Corrêa,P.G.R.; Costa,C.N.M.; Jardim,N.Y.V.; Picanço-Diniz,C.W.
Few studies have examined the influence of a low level of schooling on age-related cognitive decline in countries with wide social and economic inequalities by using the Cambridge Automated Neuropsychological Test Battery (CANTAB). The aim of the present study was to assess the influence of schooling on age-related cognitive decline using unbiased cognitive tests. CANTAB allows cognitive assessment across cultures and education levels with reduced interference of the examiner during data acquisition. Using two-way ANOVA, we assessed the influences of age and education on test scores of old adults (61–84 years of age). CANTAB tests included: Visual Sustained Attention, Reaction Time, Spatial Working Memory, Learning and Episodic Memory. All subjects had a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Age-related cognitive decline; Primary prevention; Education; Neuropsychological tests; Memory; Neuroscience; CANTAB.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400702

Paradoxical increase of exploratory behavior in the elevated plus-maze by rats exposed to two kinds of aversive stimuli
Provedor de dados: 56
Autores: Morato,S.; Brandão,M.L.
Albino rats were submitted to a 24-h period of social isolation (individual housing) combined with 0, 1, 2 or 3 twenty-four-hour periods of exposure to different vivaria (novelty) and tested in the elevated plus-maze. Results, reported as mean ± SEM for N = 12, show that the time (in seconds) spent in the open arms by rats exposed to novelty for 0, 1, 2 and 3 days was 28.3 ± 4.4, 31.6 ± 3.2, 29.1 ± 3.5 and 25.0 ± 3.3, respectively, when grouped in the same vivarium; 29.6 ± 2.7, 7.6 ± 2.1, 9.6 ± 4.4 and 28.5 ± 3.7 when grouped in different vivaria; 2.9 ± 1.1, 1.8 ± 1.0, 2.7 ± 1.1 and 0 ± 0 when isolated in the same vivarium, and 2.6 ± 1.1, 31.5 ± 8.2, 24.8 ± 4.2 and 0 ± 0 when isolated in different vivaria. The number of entries into the open and closed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elevated plus-maze; Social isolation; Novelty; Anxiety; Exploratory behavior; Aversive stimuli.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900010

Detection of the basement membrane-degrading proteolytic activity of Paracoccidioides brasiliensis after SDS-PAGE using agarose overlays containing Abz-MKALTLQ-EDDnp
Provedor de dados: 56
Autores: Puccia,R.; Juliano,M.A.; Juliano,L.; Travassos,L.R.; Carmona,A.K.
We have characterized, in the Paracoccidioides brasiliensis yeast phase, an exocellular SH-dependent serine proteinase activity against Abz-MKRLTL-EDDnp and analogous fluorescent-quenched peptides, and showed that it is also active against constituents of the basement membrane in vitro. In the present study, we separated the components of P. brasiliensis culture filtrates by electrophoresis and demonstrated that the serine-thiol exocellular proteinase has a diffuse and heterogeneous migration by SDS-PAGE, localizing in a region between 69 and 43 kDa. The hydrolytic activity was demonstrable after SDS-PAGE using buffered agarose overlays of Abz-MKALTLQ-EDDnp, following incubation at 37oC, and detection of fluorescent bands with a UV transilluminator....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P. brasiliensis; Serine-thiol proteinase; SDS-PAGE; Fluorescent-quenched peptides.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500019

Transient outward potassium current and Ca2+ homeostasis in the heart: beyond the action potential
Provedor de dados: 56
Autores: Bassani,R.A.
Normal central nervous system development relies on accurate intrinsic cellular programs as well as on extrinsic informative cues provided by extracellular molecules. Migration of neuronal progenitors from defined proliferative zones to their final location is a key event during embryonic and postnatal development. Extracellular matrix components play important roles in these processes, and interactions between neurons and extracellular matrix are fundamental for the normal development of the central nervous system. Guidance cues are provided by extracellular factors that orient neuronal migration. During cerebellar development, the extracellular matrix molecules laminin and fibronectin give support to neuronal precursor migration, while other molecules...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Central nervous system; Cerebellum; Neuron; Migration.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300010

DNA methylation patterns of candidate genes regulated by thymine DNA glycosylase in patients with TP53 germline mutations
Provedor de dados: 56
Autores: Fortes,F.P.; Kuasne,H.; Marchi,F.A.; Miranda,P.M.; Rogatto,S.R.; Achatz,M.I.
Li-Fraumeni syndrome (LFS) is a rare, autosomal dominant, hereditary cancer predisposition disorder. In Brazil, the p.R337H TP53 founder mutation causes the variant form of LFS, Li-Fraumeni-like syndrome. The occurrence of cancer and age of disease onset are known to vary, even in patients carrying the same mutation, and several mechanisms such as genetic and epigenetic alterations may be involved in this variability. However, the extent of involvement of such events has not been clarified. It is well established that p53 regulates several pathways, including the thymine DNA glycosylase (TDG) pathway, which regulates the DNA methylation of several genes. This study aimed to identify the DNA methylation pattern of genes potentially related to the TDG...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Li-Fraumeni syndrome; TP53 gene; TDG; Methylation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700610

Comparison of methods for urinary albumin determination in patients with type 1 diabetes
Provedor de dados: 56
Autores: Khawali,C.; Andriolo,A.; Ferreira,S.R.G.
We tested the correlation of the albumin-to-creatinine ratio (A/C) in an early-morning urine sample, measured with a commercial kit (DCA 2000®), with the conventional immunoturbidimetric determination in the laboratory and with overnight albumin excretion rate (reference method). Fifty-five type 1 diabetic adolescents had their first-morning urine collected on the 1st and 8th day of the period. Urinary albumin and creatinine were determined immediately using the DCA 2000® kit. Samples were also stored for laboratory analysis. To evaluate the correlation between early-morning urinary A/C ratio and overnight albumin excretion rate, 16 subjects had a timed overnight urine collection. A/C ratios determined with the DCA 2000® kit and by the laboratory method...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetic nephropathy; Microalbuminuria; Screening.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300008

Linear competitive inhibition of human tissue kallikrein by 4-aminobenzamidine and benzamidine and linear mixed inhibition by 4-nitroaniline and aniline
Provedor de dados: 56
Autores: Sousa,M.O.; Miranda,T.L.S.; Costa,E.B.; Bittar,E.R.; Santoro,M.M.; Figueiredo,A.F.S.
Hydrolysis of D-valyl-L-leucyl-L-arginine p-nitroanilide (7.5-90.0 µM) by human tissue kallikrein (hK1) (4.58-5.27 nM) at pH 9.0 and 37ºC was studied in the absence and in the presence of increasing concentrations of 4-aminobenzamidine (96-576 µM), benzamidine (1.27-7.62 mM), 4-nitroaniline (16.5-66 µM) and aniline (20-50 mM). The kinetic parameters determined in the absence of inhibitors were: Km = 12.0 ± 0.8 µM and k cat = 48.4 ± 1.0 min-1. The data indicate that the inhibition of hK1 by 4-aminobenzamidine and benzamidine is linear competitive, while the inhibition by 4-nitroaniline and aniline is linear mixed, with the inhibitor being able to bind both to the free enzyme with a dissociation constant Ki yielding an EI complex, and to the ES complex with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kinetics of human tissue kallikrein inhibition; Tissue kallikrein; 4-nitroaniline; Aniline; Benzamidine; 4-aminobenzamidine; Enzyme inhibition.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100004

Retrospective analysis of risk factors for low 1-minute Apgar scores in term neonates
Provedor de dados: 56
Autores: Yang,Congmei; Chen,Xia; Zu,Shuiling; He,Fangjie.
The current study was designed to investigate the perinatal risk factors for low 1-min Apgar scores in term neonates. We retrospectively analyzed the maternal and neonatal clinical data of 10,550 infants who were born through vaginal delivery from 37 weeks 0 days to 41 weeks 6 days of single gestation from January 2013 to July 2018. Because the 1-min Apgar score reflects neonatal status at birth, we analyzed the risk factors for low (score &lt;7) 1-min Apgar scores through logistic regression. Among these 10,550 neonates, 339 (3.2%) had low (score &lt;7) 1-min Apgar scores. Among them, 321 (94.7%) were admitted to the neonatology department for further observation or treatment. Multivariate analysis revealed that educational background, body mass index,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 1-min Apgar scores; Term neonates; Second stage of labor; Vaginal delivery; Meconium-stained amniotic fluid.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200606

FANCD2 Western blot as a diagnostic tool for Brazilian patients with Fanconi anemia
Provedor de dados: 56
Autores: Pilonetto,D.V.; Pereira,N.F.; Bitencourt,M.A.; Magdalena,N.I.R.; Vieira,E.R.; Veiga,L.B.A.; Cavalli,I.J.; Ribeiro,R.C.; Pasquini,R.
Fanconi anemia is a rare hereditary disease showing genetic heterogeneity due to a variety of mutations in genes involved in DNA repair pathways, which may lead to different clinical manifestations. Phenotypic variability makes diagnosis difficult based only on clinical manifestations, therefore laboratory tests are necessary. New advances in molecular pathogenesis of this disease led researchers to develop a diagnostic test based on Western blot for FANCD2. The objective of the present study was to determine the efficacy of this method for the diagnosis of 84 Brazilian patients with Fanconi anemia, all of whom tested positive for the diepoxybutane test, and 98 healthy controls. The FANCD2 monoubiquitinated isoform (FANCDS+/FANCD2L-) was not detected in 77...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fanconi anemia; FANCD2 Western blot; Diepoxybutane test; Monoubiquitination; Immunoblotting.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300004

IS1245 restriction fragment length polymorphism typing of Mycobacterium avium from patients admitted to a reference hospital in Campinas, Brazil
Provedor de dados: 56
Autores: Panunto,A.C.; Villares,M.C.B.; Ramos,M.C.
Mycobacterium avium is an important pathogen among immunodeficient patients, especially patients with AIDS. The natural history of this disease is unclear. Several environmental sources have been implicated as the origin of this infection. Polyclonal infection with this species is observed, challenging the understanding of its pathogenesis and treatment. In the present study 45 M. avium strains were recovered from 39 patients admitted to a reference hospital between 1996 and 1998. Species identification was performed using a species-specific nucleic acid hybridization test (AccuProbe®) from Gen-Probe®. Strains were genotyped using IS1245 restriction fragment length polymorphism typing. Blood was the main source of the organism. In one patient with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium avium; IS1245 RFLP; Mycobacteria; AIDS; Genotyping.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000017

Adaptive changes in thyroid function of female rats fed a high-fat and low-protein diet during gestation and lactation
Provedor de dados: 56
Autores: Brito,P.D.; Ramos,C.F.; Passos,M.C.F.; Moura,E.G.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High-lipid diet; Low-protein diet; Thyroid function; Lactation; Gestation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600015

Bone mineral density in juvenile systemic lupus erythematosus
Provedor de dados: 56
Autores: Castro,T.C.M.; Terreri,M.T.; Szejnfeld,V.L.; Castro,C.H.M.; Fisberg,M.; Gabay,M.; Hilário,M.O.E.
We evaluated spine bone mineral density (BMD) in Brazilian children with juvenile systemic lupus erythematosus (JSLE) in order to detect potential predictors of reduction in bone mass. A cross-sectional study of BMD at the lumbar spine level (L2-L4) was conducted on 16 female JSLE patients aged 6-17 years. Thirty-two age-matched healthy girls were used as control. BMD at the lumbar spine was measured by dual-energy X-ray absorptiometry. Weight, height and pubertal Tanner stage were determined in patients and controls. Disease duration, mean daily steroid doses, mean cumulative steroid doses and JSLE activity measured by the systemic lupus erythematosus disease activity index (SLEDAI) were determined for all JSLE patients based on their medical charts. All...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic lupus erythematosus; Children; Adolescents; Bone mineral density; Osteoporosis; Corticosteroids.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000008

Maternal serum progesterone, estradiol and estriol levels in successful dinoprostone-induced labor
Provedor de dados: 56
Autores: Konopka,C.K.; Morais,E.N.; Naidon,D.; Pereira,A.M.; Rubin,M.A.; Oliveira,J.F.; Mello,C.F.
Hormone-mediated quiescence involves the maintenance of a decreased inflammatory responsiveness. However, no study has investigated whether labor induction with prostanoids is associated with changes in the levels of maternal serum hormones. The objective of this study was to determine whether labor induction with dinoprostone is associated with changes in maternal serum progesterone, estradiol, and estriol levels. Blood samples were obtained from 81 pregnant women at term. Sixteen patients had vaginal birth after spontaneous labor, 12 required cesarean section after spontaneous labor and 16 underwent elective cesarean. Thirty-seven patients had labor induction with dinoprostone. Eligible patients received a vaginal insert of dinoprostone (10 mg) and were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Labor induction Dinoprostone; PGE2 Progesterone Estriol Estradiol.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100091

Dietary cellulose has no effect on the regeneration of hemoglobin in growing rats with iron deficiency anemia
Provedor de dados: 56
Autores: Catani,M.; Amâncio,O.M.S.; Fagundes-Neto,U.; Morais,M.B.
The objective of the present study was to determine the effect of cellulose on intestinal iron absorption in rats during recovery from iron deficiency anemia. Twenty-one-day-old male Wistar-EPM rats were fed an iron-free ration for two weeks to induce anemia. At 5 weeks of age, the rats were divided into two groups (both groups receiving 35 mg of elemental iron per kg diet): cellulose group (N = 12), receiving a diet containing 100 g of cellulose/kg and control (N = 12), receiving a diet containing no cellulose. The fresh weight of the feces collected over a 3-day period between the 15th and 18th day of dietary treatment was 10.7 ± 3.5 g in the group receiving cellulose and 1.9 ± 1.2 g in the control group (P&lt;0.001). Total food intake was higher in the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Iron deficiency; Dietary fiber; Cellulose; Iron absorption.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600002

Cytotoxic activity of BCG-activated macrophages against L929 tumor cells is nitric oxide-dependent
Provedor de dados: 56
Autores: Nascimento,F.R.F.; Ribeiro-Dias,F.; Russo,M.
The tumoricidal activity of activated macrophages has been attributed largely to the release of tumor necrosis factor (TNF), or to the production of reactive oxygen or nitrogen intermediates. The L929 tumor cell line (a murine fibroblast-like cell) when treated with actinomycin D (ActD) has been used to measure TNF&lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt; cytotoxicity. In the present study, we determined the cytotoxic activity of BCG-activated peritoneal macrophages against ActD-untreated L929 tumor cells. Furthermore, we measured the production of hydrogen peroxide (H2O2), nitric oxide (NO) and TNF by macrophages cultured in the presence or absence of L929 cells. As expected, BCG-activated macrophages produced significant amounts of H2O2 (16.0 ± 3.0 µM),...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytotoxicity; Macrophages; L929 tumor cells; Nitric oxide; TNF; Hydrogen peroxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200012

Heart rate and body weight alterations in juvenile specimens of the tropical land snail Megalobulimus sanctipauli during dormancy
Provedor de dados: 56
Autores: Rizzatti,A.C.S.; Romero,S.M.B.
The time course of heart rate and body weight alterations during the natural period of dormancy were determined in active feeding and dormant juvenile specimens of Megalobulimus sanctipauli. In both groups, heart rate markedly decreased during the first 40 days of dormancy, tending to stabilize thereafter. This time period coincided with the decrease in environmental temperature during autumn-winter. At the end of the dormancy period, surviving active feeding and dormant snails showed a significant decrease in heart rate which, however, was significantly greater in the latter group. Total body weight decreased concomitantly with heart rate in dormant snails but remained constant in active feeding snails. Body hydration induced significant increases in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dehydration; Dormancy; Heart rate; Snail; Starvation; Megalobulimus sanctipauli.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700015

Comorbidity of psychiatric disorders and symmetric distal polyneuropathy among type II diabetic outpatients
Provedor de dados: 56
Autores: Moreira,R.O.; Papelbaum,M.; Fontenelle,L.F.; Appolinario,J.C.; Ellinger,V.C.M.; Coutinho,W.F.; Zagury,L.
The objective of the present study was to establish the frequency of psychiatric comorbidity in a sample of diabetic patients with symmetric distal polyneuropathy (SDPN). Sixty-five patients with type 2 diabetes mellitus were selected consecutively to participate in the study at Instituto Estadual de Diabetes e Endocrinologia. All patients were submitted to a complete clinical and psychiatric evaluation, including the Portuguese version of the structured clinical interview for DSM-IV, the Beck Depression Inventory, the Neuropathy Symptom Score, and Neuropathy Disability Score. SDPN was identified in 22 subjects (33.8%). Patients with and without SDPN did not differ significantly regarding sociodemographic characteristics. However, a trend toward a worse...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Anxiety; Depressive symptoms; Diabetic neuropathy; Symmetric distal polyneuropathy; Psychiatric interview; Psychometric scales.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200015

Translation into Brazilian Portuguese, cultural adaptation and evaluation of the reliability of the Disabilities of the Arm, Shoulder and Hand Questionnaire
Provedor de dados: 56
Autores: Orfale,A.G.; Araújo,P.M.P.; Ferraz,M.B.; Natour,J.
The objective of the present study was to translate, adapt and validate a Brazilian Portuguese version of the Disabilities of the Arm, Shoulder and Hand (DASH) Questionnaire. The study was carried out in two steps. The first was to translate the DASH into Portuguese and to perform cultural adaptation and the second involved the determination of the reliability and validity of the DASH for the Brazilian population. For this purpose, 65 rheumatoid arthritis patients of either sex (according to the classification criteria of the American College of Rheumatology), ranging in age from 18 to 60 years and presenting no other diseases involving the upper limbs, were interviewed. The patients were selected consecutively at the rheumatology outpatient clinic of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disabilities of the Arm; Shoulder and Hand (DASH); Translation into Brazilian Portuguese; Disability; Questionnaire; Arthritis; Upper limb; Validation; Arm.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200018

Association of ApoE polymorphisms with prevalent hypertension in 1406 older adults: the Bambuí Health Aging Study (BHAS)
Provedor de dados: 56
Autores: Fuzikawa,A.K.; Peixoto,S.V.; Taufer,M.; Moriguchi,E.H.; Lima-Costa,M.F.
Tipo: Info:eu-repo/semantics/article
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700015

Ca2+ dependence of gluconeogenesis stimulation by glucagon at different cytosolic NAD+-NADH redox potentials
Provedor de dados: 56
The influence of Ca2+ on hepatic gluconeogenesis was measured in the isolated perfused rat liver at different cytosolic NAD+-NADH potentials. Lactate and pyruvate were the gluconeogenic substrates and the cytosolic NAD+-NADH potentials were changed by varying the lactate to pyruvate ratios from 0.01 to 100. The following results were obtained: a) gluconeogenesis from lactate plus pyruvate was not affected by Ca2+-free perfusion (no Ca2+ in the perfusion fluid combined with previous depletion of the intracellular pools); gluconeogenesis was also poorly dependent on the lactate to pyruvate ratios in the range of 0.1 to 100; only for a ratio equal to 0.01 was a significantly smaller gluconeogenic activity observed in comparison to the other ratios. b) In the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rat liver perfusion; Glucagon; Gluconeogenesis; Ca2+; NAD+-NADH redox potential.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700002

Antibody response to pneumococcal capsular polysaccharide vaccine in Down syndrome patients
Provedor de dados: 56
Autores: Costa-Carvalho,B.T.; Martinez,R.M.A.; Dias,A.T.N.; Kubo,C.A.; Barros-Nunes,P.; Leiva,L.; Solé,D.; Carneiro-Sampaio,M.M.S.; Naspitz,C.K.; Sorensen,R.U.
The majority of children with Down syndrome (DS) tend to have frequent bacterial infections including recurrent respiratory infections. Our objective was to evaluate the production of antibodies to pneumococcal polysaccharide antigens after active immunization in DS subjects. IgG antibodies to pneumococcal serotypes (1, 3, 6B, 9V, and 14) were measured before and 6 weeks after immunization with a 23-valent pneumococcal vaccine (Pneumo23®, Pasteur-Merrieux) in 6- to 13-year-old DS children (N = 17) and in aged-matched normal controls (N = 30). An adequate response was defined as a 4-fold increase over baseline or a post-immunization level of specific pneumococcal serotype antibody  &gt; or = 1.3 µg/mL. After immunization, all DS children had an increase in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Down syndrome; Immunoglobulin; IgG subclass; Polysaccharide vaccine; Streptococcus pneumoniae; Pneumococcal antibodies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200010

Community-based transconjunctival marginal rotation for cicatricial trachoma in Indians from the Upper Rio Negro basin
Provedor de dados: 56
Autores: Soares,O.E.; Cruz,A.A.V.
The objective of the present study was to describe, for the first time in Brazil, the use by a non-ophthalmologist of a community-based marginal rotation procedure by a posterior approach in the indigenous population from the Upper Rio Negro basin. Seventy-three upper eyelids of 46 Indians (11 males and 35 females) with cicatricial upper eyelid entropion and trichiasis were operated in the Indian communities using a marginal rotational procedure by a posterior approach by a non-ophthalmologist physician who had general surgery experience but only an extremely short period (one week) of ophthalmic training. Subjects were reevaluated 6 months after surgery. Results were classified according to the presence and location of residual trichiasis and symptoms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trachoma; Community-based surgery; Marginal rotation; Trichiasis; Entropion.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500007

Single nucleotide polymorphisms predisposing to asthma in children of Mauritian Indian and Chinese Han ethnicity
Provedor de dados: 56
Autores: Ramphul,K.; Lv,J.; Hua,L.; Liu,Q.H.; Fang,D.Z.; Ji,R.X.; Bao,Y.X.
Our objective was to investigate the distributions of six single nucleotide polymorphisms (SNPs) MS4A2 E237G, MS4A2 C-109T, ADRB2 R16G, IL4RA I75V,IL4 C-590T, and IL13 C1923T in Mauritian Indian and Chinese Han children with asthma. This case-control association study enrolled 382 unrelated Mauritian Indian children, 193 with asthma and 189 healthy controls, and 384 unrelated Chinese Han children, 192 with asthma and 192 healthy controls. The SNP loci were genotyped using polymerase chain reaction (PCR)-restriction fragment length polymorphism for the Chinese Han samples and TaqMan real-time quantitative PCR for the Mauritian Indian samples. In the Mauritian Indian children, there was a significant difference in the distribution of IL13 C1923T between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Single nucleotide polymorphisms; Mauritian Indian; Chinese Han.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500394

Different methods of calculating ankle-brachial index in mid-elderly men and women: the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil)
Provedor de dados: 56
Autores: Miname,M.; Bensenor,I.M.; Lotufo,P.A.
The ankle-brachial index (ABI) is a marker of subclinical atherosclerosis related to health-adverse outcomes. ABI is inexpensive compared to other indexes, such as coronary calcium score and determination of carotid artery intima-media thickness (IMT). Our objective was to identify how the ABI can be applied to primary care. Three different methods of calculating the ABI were compared among 13,921 men and women aged 35 to 74 years who were free of cardiovascular diseases and enrolled in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). The ABI ratio had the same denominator for the three categories created (the highest value for arm systolic blood pressure), and the numerator was based on the four readings for leg systolic blood pressure: the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peripheral artery disease; Risk factors; Atherosclerosis; Cardiovascular risk; Primary care.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200701

Phenotyping and genotyping methods applied to investigate the relatedness of Brazilian isolates of Enterobacter cloacae
Provedor de dados: 56
Autores: Darini,A.L.C.; Magalhães,V.D.; Levy,C.L.; Barth,A.L.; Coscina,A.L.
In order to evaluate the resolving power of several typing methods to identify relatedness among Brazilian strains of Enterobacter cloacae, we selected twenty isolates from different patients on three wards of a University Hospital (Orthopedics, Nephrology, and Hematology). Traditional phenotyping methods applied to isolates included biotyping, antibiotic sensitivity, phage-typing, and O-serotyping. Plasmid profile analysis, ribotyping, and macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) were used as genotyping methods. Sero- and phage-typing were not useful since the majority of isolates could not be subtyped by these methods. Biotyping, antibiogram and plasmid profile permitted us to classify the samples into different groups...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enterobacter cloacae; Phage-typing; Serotyping; Ribotyping; Pulsed-field gel electrophoresis; Nosocomial infection.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900004

Loss of resting bradycardia with detraining is associated with intrinsic heart rate changes
Provedor de dados: 56
Autores: Evangelista,F.S.; Martuchi,S.E.D.; Negrão,C.E.; Brum,P.C.
The mechanisms underlying the loss of resting bradycardia with detraining were studied in rats. The relative contribution of autonomic and non-autonomic mechanisms was studied in 26 male Wistar rats (180-220 g) randomly assigned to four groups: sedentary (S, N = 6), trained (T, N = 8), detrained for 1 week (D1, N = 6), and detrained for 2 weeks (D2, N = 6). T, D1 and D2 were treadmill trained 5 days/week for 60 min with a gradual increase towards 50% peak VO2. After the last training session, D1 and D2 were detrained for 1 and 2 weeks, respectively. The effect of the autonomic nervous system in causing training-induced resting bradycardia and in restoring heart rate (HR) to pre-exercise training level (PET) with detraining was examined indirectly after...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Exercise training; Cardiac autonomic balance; Heart rate; Peak oxygen uptake; Detraining.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700018

Normative data for the Brazilian population in the Boston Diagnostic Aphasia Examination: influence of schooling
Provedor de dados: 56
Autores: Radanovic,M.; Mansur,L.L.; Scaff,M.
In Neurolinguistics, the use of diagnostic tests developed in other countries can create difficulties in the interpretation of results due to cultural, demographic and linguistic differences. In a country such as Brazil, with great social contrasts, schooling exerts a powerful influence on the abilities of normal individuals. The objective of the present study was to identify the influence of schooling on the performance of normal Brazilian individuals in the Boston Diagnostic Aphasia Examination (BDAE), in order to obtain reference values for the Brazilian population. We studied 107 normal subjects ranging in age from 15 to 84 years (mean ± SD = 47.2 ± 17.6 years), with educational level ranging from 1 to 24 years (9.9 ± 4.8 years). Subjects were compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Boston Diagnostic Aphasia Examination; Schooling; Brazilian population.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100019

Recombinant 60-kDa heat shock protein from Paracoccidioides brasiliensis: is it a good antigen for serological diagnosis of paracoccidioidomycosis?
Provedor de dados: 56
Autores: Peron,G.; Fernandes,F.F.; Landgraf,T.N.; Martinez,R.; Panunto-Castelo,A.
Paracoccidioides brasiliensis and P. lutzii are fungi that cause paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in South America. For serological diagnosis, although 43-kDa glycoprotein (gp43) is regarded as highly specific for PCM, the occurrence of false negative reactions in sera from patients infected with P. lutzii suggests that preparation with only one antigen is not recommended. Heat shock proteins are feasible alternatives as a second antigen because they are often highly immunogenic. In this study, we evaluated the usefulness of recombinant 60-kDa heat shock protein from P. brasiliensis (rPbHsp60) for the serological diagnosis of PCM. Using western blotting assay, we observed that 77.3% of the sera from PCM patients were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paracoccidioidomycosis; Heat shock protein; Serodiagnosis; ELISA; Western blotting.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400705

Early detection of metabolic and energy disorders by thermal time series stochastic complexity analysis
Provedor de dados: 56
Autores: Lutaif,N.A.; Palazzo Jr,R.; Gontijo,J.A.R.
Maintenance of thermal homeostasis in rats fed a high-fat diet (HFD) is associated with changes in their thermal balance. The thermodynamic relationship between heat dissipation and energy storage is altered by the ingestion of high-energy diet content. Observation of thermal registers of core temperature behavior, in humans and rodents, permits identification of some characteristics of time series, such as autoreference and stationarity that fit adequately to a stochastic analysis. To identify this change, we used, for the first time, a stochastic autoregressive model, the concepts of which match those associated with physiological systems involved and applied in male HFD rats compared with their appropriate standard food intake age-matched male controls...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Time series temperature; Thermal homeostasis; High-fat diet; Metabolic disorder predictor; Autoregressive models.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100070

Acute electrophysiologic consequences of pyridostigmine inhibition of cholinesterase in humans
Provedor de dados: 56
Autores: Zimerman,L.I.; Liberman,A.; Castro,R.R.T.; Ribeiro,J.P.; Nóbrega,A.C.L.
The cardiovascular electrophysiologic basis for the action of pyridostigmine, an acetylcholinesterase inhibitor, has not been investigated. The objective of the present study was to determine the cardiac electrophysiologic effects of a single dose of pyridostigmine bromide in an open-label, quasi-experimental protocol. Fifteen patients who had been indicated for diagnostic cardiac electrophysiologic study underwent two studies just before and 90-120 min after the oral administration of pyridostigmine (45 mg). Pyridostigmine was well tolerated by all patients. Wenckebach nodal anterograde atrioventricular point and basic cycle were not altered by pyridostigmine. Sinus recovery time (ms) was shorter during a 500-ms cycle stimulation (pre: 326 ± 45 vs post:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac electrophysiology; Parasympathetic nervous system; Cholinesterase inhibitors; Cardiovascular disease; Pyridostigmine bromide; Autonomic nervous system.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200013

Anticonvulsant and antiarrhythmic effects of nifedipine in rats prone to audiogenic seizures
Provedor de dados: 56
Autores: Damasceno,D.D.; Ferreira,A.J.; Doretto,M.C.; Almeida,A.P.
Calcium ion participates in the regulation of neural transmission and the presynaptic release of neurotransmitters. It is also involved in epileptic events, cardiac arrhythmias and abnormal conduction of stimuli. The purpose of the present study was to evaluate the effects of nifedipine, a calcium channel blocker, on epileptic seizures and on reperfusion arrhythmias in rats prone to audiogenic epileptic seizures (Wistar audiogenic rats, WAR) and in normal Wistar rats (N = 6/group). The seizure severity index was applied after an intraperitoneal injection of 20 or 40 mg/kg nifedipine (N20 and N40 groups, respectively). The Langendorff technique was used to analyze cardiac function, as well as the incidence and severity of the reperfusion arrhythmias after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nifedipine; Calcium channel antagonism; Audiogenic seizures; Epilepsy; Arrhythmias.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100010

Rapid, reliable and inexpensive quality assessment of biotinylated cRNA
Provedor de dados: 56
Autores: Zander,T.; Yunes,J.A.; Cardoso,A.A.; Nadler,L.M.
The interpretation of oligonucleotide array experiments depends on the quality of the target cRNA used. cRNA target quality is assessed by quantitative analysis of the representation of 5' and 3' sequences of control genes using commercially available Test arrays. The Test array provides an economically priced means of determining the quality of labeled target prior to analysis on whole genome expression arrays. This manuscript validates the use of a duplex RT-PCR assay as a faster (6 h) and less expensive (&lt;US$10) but equally accurate alternative to the Test arrays in determining biotinylated cRNA quality. Forty-one different cRNA samples were hybridized to HG-U133A microarrays from Affymetrix. Ten cRNA samples with a ß-actin 3'/5' ratio &gt;6 were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Microarray; Biotinylated cRNA; Probe quality; 5' Representation; 3'/5' Ratio.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500004

Role of non-nitric oxide non-prostaglandin endothelium-derived relaxing factor(s) in bradykinin vasodilation
Provedor de dados: 56
Autores: Resende,A.C.; Ballejo,G.; Salgado,M.C.O.
The most conspicuous effect of bradykinin following its administration into the systemic circulation is a transient hypotension due to vasodilation. In the present study most of the available evidence regarding the mechanisms involved in bradykinin-induced arterial vasodilation is reviewed. It has become firmly established that in most species vasodilation in response to bradykinin is mediated by the release of endothelial relaxing factors following the activation of B2-receptors. Although in some cases the action of bradykinin is entirely mediated by the endothelial release of nitric oxide (NO) and/or prostacyclin (PGI2), a large amount of evidence has been accumulated during the last 10 years indicating that a non-NO/PGI2 factor accounts for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostaglandin; Bradykinin; Nitric oxide; Cytochrome P450; Potassium channels; Endothelium-dependent vasodilation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900017

The biological effects of high-pressure gas on the yeast transcriptome
Provedor de dados: 56
Autores: Matsuoka,H.; Suzuki,Y.; Iwahashi,H.; Arao,T.; Suzuki,Y.; Tamura,K.
The aim of the present study was to examine the feasibility of DNA microarray technology in an attempt to construct an evaluation system for determining gas toxicity using high-pressure conditions, as it is well known that pressure increases the concentration of a gas. As a first step, we used yeast (Saccharomyces cerevisiae) as the indicator organism and analyzed the mRNA expression profiles after exposure of yeast cells to nitrogen gas. Nitrogen gas was selected as a negative control since this gas has low toxicity. Yeast DNA microarray analysis revealed induction of genes whose products were localized to the membranes, and of genes that are involved in or contribute to energy production. Furthermore, we found that nitrogen gas significantly affected the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA microarray; Yeast; Hydrostatic pressure; Gas pressure; Nitrogen gas.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800016

Cissampelos sympodialis Eichl (Menispermaceae) leaf extract induces interleukin-10-dependent inhibition of Trypanosoma cruzi killing by macrophages
Provedor de dados: 56
Autores: Alexandre-Moreira,M.S.; Freire-de-Lima,C.G.; Trindade,M.N.; Castro-Faria-Neto,H.C.; Piuvezam,M.R.; Peçanha,L.M.T.
The aqueous fraction of the ethanolic extract (AFL) of Cissampelos sympodialis Eichl (Menispermaceae), popularly known as milona, has been shown to have both immunosuppressive and anti-inflammatory effects. In the present study we investigated the modulation of macrophage antimicrobicidal activity by in vitro treatment with the extract from C. sympodialis. Normal and thioglycolate-elicited mouse peritoneal macrophages were infected in vitro with the protozoan Trypanosoma cruzi DM28c clone. We observed that the AFL (used at doses ranging from 13 to 100 µg/ml) increased T. cruzi growth and induced a 75% reduction in nitric oxide production. This inhibition could be mediated by the stimulation of macrophage interleukin-10 (IL-10) secretion since the in vitro...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cissampelos sympodialis; Macrophage; Trypanosoma cruzi; Nitric oxide; Interleukin-10.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200006

Effect of selective β-adrenoceptor blockade and surgical resection of the celiac-superior mesenteric ganglion complex on delayed liquid gastric emptying induced by dipyrone, 4-aminoantipyrine, and antipyrine in rats
Provedor de dados: 56
Autores: Vinagre,A.M.; Collares,E.F.
There is evidence for participation of peripheral β-adrenoceptors in delayed liquid gastric emptying (GE) induced in rats by dipyrone (Dp), 4-aminoantipyrine (AA), and antipyrine (At). The present study aimed to determine whether β-adrenoceptors are involved in delayed GE induced by phenylpyrazole derivatives and the role of the prevertebral sympathetic nervous system in this condition. Male Wistar rats weighing 220-280 g were used in the study. In the first experiment rats were intravenously pretreated with vehicle (V), atenolol 30 mg/kg (ATE, β1-adrenergic antagonist), or butoxamine 25 mg/kg (BUT, β2-adrenergic antagonist). In the second experiment, rats were pretreated with V or SR59230A 2 mg/kg (SRA, β3-adrenergic antagonist). In the third experiment,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Dipyrone; 4-aminoantipyrine; Antipyrine; Β2-Adrenoceptor; Prevertebral sympathetic ganglia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300603

Electrocardiographic changes during low-dose, short-term therapy of cutaneous leishmaniasis with the pentavalent antimonial meglumine
Provedor de dados: 56
Autores: Ribeiro,A.L.P.; Drummond,J.B.; Volpini,A.C.; Andrade,A.C.; Passos,V.M.A.
The pentavalent antimonial (Sb5+) meglumine is the drug of choice for the treatment of cutaneous leishmaniasis (CL) in Brazil. Although the cardiotoxicity of high-dose, long-term Sb5+ therapy is well known, the use of low-dose, short-term meglumine has been considered to be safe and relatively free from significant cardiac effects. In order to investigate the cardiotoxicity of low-dose, short-term therapy with meglumine in cutaneous leishmaniasis, 62 CL patients treated with meglumine were studied. A standard ECG was obtained before and immediately after the first cycle of treatment (15 mg Sb5+ kg-1 day-1). The electrocardiographic interpretation was carried out blindly by two investigators using the Minnesota Code. There were no significant differences in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cutaneous leishmaniasis; Electrocardiography; QT interval.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300008

Modulation of the perception of temporal order by attentional and pre-attentional factors
Provedor de dados: 56
Autores: Haddad Jr.,H.; Carreiro,L.R.R.; Baldo,M.V.C.
When two stimuli are presented simultaneously to an observer, the perceived temporal order does not always correspond to the actual one. In three experiments we examined how the location and spatial predictability of visual stimuli modulate the perception of temporal order. Thirty-two participants had to report the temporal order of appearance of two visual stimuli. In Experiment 1, both stimuli were presented at the same eccentricity and no perceptual asynchrony between them was found. In Experiment 2, one stimulus was presented close to the fixation point and the other, peripheral, stimulus was presented in separate blocks in two eccentricities (4.8º and 9.6º). We found that the peripheral stimulus was perceived to be delayed in relation to the central...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Temporal order judgment; Attention; Visual perception; Psychophysics.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800016

Percent body fat is a better predictor of cardiovascular risk factors than body mass index
Provedor de dados: 56
Autores: Zeng,Qiang; Dong,Sheng-Yong; Sun,Xiao-Nan; Xie,Jing; Cui,Yi.
The objective of the present study was to evaluate the predictive values of percent body fat (PBF) and body mass index (BMI) for cardiovascular risk factors, especially when PBF and BMI are conflicting. BMI was calculated by the standard formula and PBF was determined by bioelectrical impedance analysis. A total of 3859 ambulatory adult Han Chinese subjects (2173 males and 1686 females, age range: 18-85 years) without a history of cardiovascular diseases were recruited from February to September 2009. Based on BMI and PBF, they were classified into group 1 (normal BMI and PBF, N = 1961), group 2 (normal BMI, but abnormal PBF, N = 381), group 3 (abnormal BMI, but normal PBF, N = 681), and group 4 (abnormal BMI and PBF, N = 836). When age, gender, lifestyle,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Body composition; Body mass index; Cardiovascular disease; Risk factor; Percent body fat; Bioelectrical impedance.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700005

Laminin concentration in ascites of patients with hepatic cirrhosis and peritoneal carcinomatosis
Provedor de dados: 56
Autores: Catarino,R.M.; Lopes,J.D.; Forones,N.M.; Parise,E.R.
Laminin levels in ascitic fluid have been proposed as a marker for neoplastic ascites. We compared the concentration of laminin in serum and in ascitic fluid from patients with hepatic cirrhosis and peritoneal carcinomatosis and assessed the diagnostic value of serum laminin levels in differentiating neoplastic from benign ascites. Laminin concentrations were determined by ELISA with antibodies against laminin extracted from the human placenta, in patients with ascites due to peritoneal carcinomatosis (N = 20) and hepatic cirrhosis (N = 33). Patients with infected or hemorrhagic ascites were excluded. The receiver operating characteristic curve was used to determine the sensitivity and specificity of serum laminin for the diagnosis of neoplastic ascites....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascites laminin; Serum laminin; Peritoneal carcinomatosis; Cirrhosis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200016

Nitrergic modulation of vasopressin, oxytocin and atrial natriuretic peptide secretion in response to sodium intake and hypertonic blood volume expansion
Provedor de dados: 56
Autores: Ventura,R.R.; Gomes,D.A.; Reis,W.L.; Elias,L.L.K.; Castro,M.; Valença,M.M.; Carnio,E.C.; Rettori,V.; McCann,S.M.; Antunes-Rodrigues,J.
The central nervous system plays an important role in the control of renal sodium excretion. We present here a brief review of physiologic regulation of hydromineral balance and discuss recent results from our laboratory that focus on the participation of nitrergic, vasopressinergic, and oxytocinergic systems in the regulation of water and sodium excretion under different salt intake and hypertonic blood volume expansion (BVE) conditions. High sodium intake induced a significant increase in nitric oxide synthase (NOS) activity in the medial basal hypothalamus and neural lobe, while a low sodium diet decreased NOS activity in the neural lobe, suggesting that central NOS is involved in the control of sodium balance. An increase in plasma concentrations in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vasopressin; Oxytocin; ANP; Nitric oxide; Blood volume expansion; Sodium diet.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900011

Erythrocyte phosphoglucomutase activity of bipolar I patients currently using lithium or carbamazepine
Provedor de dados: 56
Autores: Montero-Lomelí,M.; Galvão,D.; Morais,B.B.; Nardi,A.E.
Lithium has been used for the last five decades to treat bipolar disorder, but the molecular basis of its therapeutic effect is unknown. Phosphoglucomutase is a key enzyme in the metabolism of glycogen. In yeast, rabbit and human HEK293 cells, it is inhibited by lithium in the therapeutic concentration range. We measured the phosphoglucomutase activity in erythrocytes and the inhibitor constant for lithium in a population of healthy subjects and compared them to those of bipolar patients treated with lithium or carbamazepine. The specific activity of phosphoglucomutase measured in vitro in erythrocytes from control subjects presented a normal distribution, with the difference between the lowest and the highest activity being approximately 2-fold (0.53-1.10...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phosphoglucomutase; Lithium; Carbamazepine; Bipolar disorder.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100003

Bone marrow mesenchymal stem cells overexpressing human basic fibroblast growth factor increase vasculogenesis in ischemic rats
Provedor de dados: 56
Autores: Zhang,J.C.; Zheng,G.F.; Wu,L.; Ou Yang,L.Y.; Li,W.X.
Administration or expression of growth factors, as well as implantation of autologous bone marrow cells, promote in vivo angiogenesis. This study investigated the angiogenic potential of combining both approaches through the allogenic transplantation of bone marrow-derived mesenchymal stem cells (MSCs) expressing human basic fibroblast growth factor (hbFGF). After establishing a hind limb ischemia model in Sprague Dawley rats, the animals were randomly divided into four treatment groups: MSCs expressing green fluorescent protein (GFP-MSC), MSCs expressing hbFGF (hbFGF-MSC), MSC controls, and phosphate-buffered saline (PBS) controls. After 2 weeks, MSC survival and differentiation, hbFGF and vascular endothelial growth factor (VEGF) expression, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiogenesis; Basic fibroblast growth factor; Bone marrow mesenchymal stem cells; Gene transfection; Ischemia.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000886

Effect of neurotrophic factor, MDP, on rats’ nerve regeneration
Provedor de dados: 56
Autores: Fornazari,A.A.; de Rezende,M.R.; Mattar Jr.,R.; Taira,R.I.; dos Santos,G.B.; Paulos,R.G.
Our objective was to determine the immune-modulating effects of the neurotrophic factor N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) on median nerve regeneration in rats. We used male Wistar rats (120-140 days of age, weighing 250-332 g) and compared the results of three different techniques of nerve repair: 1) epineural neurorrhaphy using sutures alone (group S - 10 rats), 2) epineural neurorrhaphy using sutures plus fibrin tissue adhesive (FTA; group SF - 20 rats), and 3) sutures plus FTA, with MDP added to the FTA (group SFM - 20 rats). Functional assessments using the grasp test were performed weekly for 12 weeks to identify recovery of flexor muscle function in the fingers secondary to median nerve regeneration. Histological analysis was also...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Grasping test; Neurotrophic factor; N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP).
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400009

Enhancement of declarative memory associated with emotional content in a Brazilian sample
Provedor de dados: 56
Autores: Frank,J.E.; Tomaz,C.
Several studies have documented that emotional arousal may enhance long-term memory. This is an adaptation of a paradigm previously used in North American and European samples in investigations of the influence of emotion on long-term retention. A sample of 46 healthy adults of high and low educational levels watched a slide presentation of stories. A randomly assigned group watched a story with an arousing content and another group watched a neutral story. The stories were matched for structure and comprehensibility and the set and order of the 11 slides were the same in both conditions. Immediately after viewing the slide presentation, the participants were asked to rate the emotionality of the narrative. The arousing narrative was rated as being more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Memory; Emotion; Stories; Brazilian.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200013

Factors associated with the development of renal complications of diabetes mellitus in São Paulo city
Provedor de dados: 56
The incidence of diabetic end-stage renal failure (ESRF) varies worldwide and risk factors have been demonstrated in several populations. The objective of the present study was to identify possible factors associated with the risk of development of ESRF in patients with diabetes mellitus (DM). Two groups of diabetic subjects were included in a case-control study: 1) one group was submitted to renal replacement therapies, attending dialysis centers in São Paulo city and 2) the same number of controls without clinical nephropathy (two negative dipstick tests for urine protein), matched for duration of DM, were obtained from an outpatient clinic. A standardized questionnaire was used by a single investigator and additional data were obtained from the medical...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chronic renal failure; Diabetes mellitus; Diabetic nephropathy; Associated factors.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600006

The mechanism of gentisic acid-induced relaxation of the guinea pig isolated trachea: the role of potassium channels and vasoactive intestinal peptide receptors
Provedor de dados: 56
Autores: Cunha,J.F.; Campestrini,F.D.; Calixto,J.B.; Scremin,A.; Paulino,N.
We examined some of the mechanisms by which the aspirin metabolite and the naturally occurring metabolite gentisic acid induced relaxation of the guinea pig trachea in vitro. In preparations with or without epithelium and contracted by histamine, gentisic acid caused concentration-dependent and reproducible relaxation, with mean EC50 values of 18 µM and Emax of 100% (N = 10) or 20 µM and Emax of 92% (N = 10), respectively. The relaxation caused by gentisic acid was of slow onset in comparison to that caused by norepinephrine, theophylline or vasoactive intestinal peptide (VIP). The relative rank order of potency was: salbutamol 7.9 &gt; VIP 7.0 &gt; gentisic acid 4.7 &gt; theophylline 3.7. Gentisic acid-induced relaxation was markedly reduced (24 ± 7.0, 43...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gentisic acid; Potassium channels; Vasoactive intestinal peptide; VIP; Trachea; Smooth muscle.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300013

The use of polymerase chain reaction for early diagnosis of tuberculosis in Mycobacterium tuberculosis culture
Provedor de dados: 56
Autores: Chagas,M.; Silva,R.M. da; Bazzo,M.L.; Santos,J.I. dos.
Early diagnosis plays a vital role in controlling tuberculosis. The conventional methodology is slow, with results taking several weeks, in addition to having low sensitivity, especially in clinical paucibacillary samples. The objective of this study was to evaluate the use of polymerase chain reaction (PCR) on solid medium culture for a rapid diagnosis of tuberculosis, mainly in cases of negative sputum smears. Forty sputum samples were collected from inpatients with tuberculosis treated for less than 2 days. Bacilloscopy, PCR for sputum, culture on Löwestein-Jensen (LJ) solid medium, and daily PCR from culture were performed on each sample. DNA extracted from the BCG vaccine, which contains attenuated bacillus Calmette-Guérin, was used as the positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Mycobacteria; PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600004

Expression of myo-inositol cotransporters in the sciatic nerve and dorsal root ganglia in experimental diabetes
Provedor de dados: 56
Autores: Farias,V.X.; Uchoa,P.N.; Aquino,C.P.; Britto,L.R.G.; Fonteles,M.C.; Leal-Cardoso,J.H.; Silva-Alves,K.S.; Havt,A.; Prata,M.M.G.; Heimark,D.B.; Nascimento,N.R.F.; Santos,C.F.
The transport of myo-inositol is the main mechanism for the maintenance of its high intracellular levels. We aimed to measure the mRNA and protein levels of myo-inositol cotransporters in the sciatic nerve (SN) and dorsal root ganglia (DRG) during experimental diabetes. Streptozotocin-induced (STZ; 4, 8, and 12 weeks; 65 mg/kg; ip) diabetic rats (DB) and age-matched euglycemic (E) rats were used for the analysis of mRNA and protein levels of sodium myo-inositol cotransporters 1, 2 (SMIT1, SMIT2) or H+/myo-inositol cotransporter (HMIT). There was a significant reduction in the mRNA levels for SMIT1 in the SN and DRG (by 36.9 and 31.0%) in the 4-week DB (DB4) group compared to the E group. SMIT2 was not expressed in SN. The mRNA level for SMIT2 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetic neuropathy; Inositol metabolism; Peripheral nervous tissues.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600609

Effect and the probable mechanisms of silibinin in regulating insulin resistance in the liver of rats with non-alcoholic fatty liver
Provedor de dados: 56
Autores: Yao,Jiayin; Zhi,Min; Gao,Xiang; Hu,Pinjin; Li,Chujun; Yang,Xiaobo.
Our previous study has shown that reduced insulin resistance (IR) was one of the possible mechanisms for the therapeutic effect of silibinin on non-alcoholic fatty liver disease (NAFLD) in rats. In the present study, we investigated the pathways of silibinin in regulating hepatic glucose production and IR amelioration. Forty-five 4- to 6-week-old male Sprague Dawley rats were divided into a control group, an HFD group (high-fat diet for 6 weeks) and an HFD + silibinin group (high-fat diet + 0.5 mg kg-1·day-1 silibinin, starting at the beginning of the protocol). Both subcutaneous and visceral fat was measured. Homeostasis model assessment-IR index (HOMA-IR), intraperitoneal glucose tolerance test and insulin tolerance test (ITT) were performed. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-alcoholic fatty liver disease; Insulin resistance; Silibinin; Visceral obesity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300270

Reduction of blood nitric oxide levels is associated with clinical improvement of the chronic pelvic pain related to endometriosis
Provedor de dados: 56
Autores: Rocha,M.G.; Gomes,V.A.; Tanus-Santos,J.E.; Rosa-e-Silva,J.C.; Candido-dos-Reis,F.J.; Nogueira,A.A.; Poli-Neto,O.B.
The objective of this prospective study was to determine the plasma levels of nitric oxide (NO) in women with chronic pelvic pain secondary to endometriosis (n=24) and abdominal myofascial pain syndrome (n=16). NO levels were measured in plasma collected before and 1 month after treatment. Pretreatment NO levels (μM) were lower in healthy volunteers (47.0±12.7) than in women with myofascial pain (64.2±5.0, P=0.01) or endometriosis (99.5±12.9, P&lt;0.0001). After treatment, plasma NO levels were reduced only in the endometriosis group (99.5±12.9 vs 61.6±5.9, P=0.002). A correlation between reduction of pain intensity and reduction of NO level was observed in the endometriosis group [correlation = 0.67 (95%CI = 0.35 to 0.85), P&lt;0.0001]. Reduction of NO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic pelvic pain; Endometriosis; Abdominal myofascial pain syndrome; Nitric oxide; Inflammation; Central sensitization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400363

Newborn screening for biotinidase deficiency in Brazil: biochemical and molecular characterizations
Provedor de dados: 56
Autores: C. Neto,E.; Schulte,J.; Rubim,R.; Lewis,E.; DeMari,J.; Castilhos,C.; Brites,A.; Giugliani,R.; Jensen,K.P.; Wolf,B.
Biotinidase deficiency is an inherited metabolic disorder characterized by neurological and cutaneous symptoms. Fortunately, it can be treated and the symptoms prevented by oral administration of the vitamin biotin. Using dried blood-soaked filter paper cards, biotinidase activity was determined in the sera of 225,136 newborns in Brazil. Mutation analysis performed on DNA from 21 babies with low serum biotinidase activity confirmed that 3 had profound biotinidase deficiency (less than 10% of mean normal sera biotinidase activity), 10 had partial biotinidase deficiency (10 to 30% of mean normal serum activity), 1 was homozygous for partial biotinidase deficiency, 4 were heterozygous for either profound or partial deficiency, and 3 were normal. Variability...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Newborn screening; Biotinidase deficiency; Biotinidase; Mutations; Enzyme assay.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300001

Identification and in vitro production of Lactobacillus antagonists from women with or without bacterial vaginosis
Provedor de dados: 56
Autores: Branco,K.M.G.R.; Nardi,R.M.D.; Moreira,J.L.S.; Nunes,A.C.; Farias,L.M.; Nicoli,J.R.; Carvalho,M.A.R.
Lactobacilli isolated from the vaginal tract of women with and without bacterial vaginosis (BV) were identified and characterized for the production of antagonists. Bacterial samples were isolated from healthy women (N = 16), from patients with clinical complaints but without BV (N = 30), and from patients with BV (N = 32). Identification was performed using amplified ribosomal DNA restriction analysis. Production of antagonistic compounds was evaluated by the double-layer diffusion technique using Gram-positive (N = 9) and Gram-negative bacteria (N = 6) as well as yeast (N = 5) as indicator strains. Of a total of 147 isolates, 133 were identified as pertaining to the genus Lactobacillus. Lactobacillus crispatus was the species most frequently recovered,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus; Bacterial vaginosis; Antagonism; Hydrogen peroxide; Identification.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400003

Preliminary data for performance in hue ordering tests during pregnancy
Provedor de dados: 56
Autores: Calandrini,T.S.S.; Miquilini,L.; Laranjeiras-Neto,M.R.; Tongu,M.T.S.; Silva,M.P.; Souza,G.S.; Cortes,M.I.T.
The visual system of women changes during pregnancy. Few reports have addressed the effects of pregnancy on color vision. We aimed to compare the color vision of women in the first, second, and third trimesters of pregnancy. Fifty women were divided into first (n=10), second (n=10), third trimester pregnancy groups (n=10), and non-pregnant group (n=20). We used the Farnsworth D15 and Lanthony desaturated D15 (D15d) tests. The hue ordering quantified the amount of error (C-index) and the chromatic selectivity of the errors (S-index). Bland-Altman analysis was applied to the hue ordering data. No difference was found for Farnsworth D15 test results obtained from the pregnant groups and the non-pregnant group (P&lt;0.0083). For the Lanthony D15 desaturated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pregnancy; Visual system; Color vision; Psychophysics.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200606

Aurapten, a coumarin with growth inhibition against Leishmania major promastigotes
Provedor de dados: 56
Autores: Napolitano,H.B.; Silva,M.; Ellena,J.; Rodrigues,B.D.G.; Almeida,A.L.C.; Vieira,P.C.; Oliva,G.; Thiemann,O.H.
Several natural compounds have been identified for the treatment of leishmaniasis. Among them are some alkaloids, chalcones, lactones, tetralones, and saponins. The new compound reported here, 7-geranyloxycoumarin, called aurapten, belongs to the chemical class of the coumarins and has a molecular weight of 298.37. The compund was extracted from the Rutaceae species Esenbeckia febrifuga and was purified from a hexane extract starting from 407.7 g of dried leaves and followed by four silica gel chromatographic fractionation steps using different solvents as the mobile phase. The resulting compound (47 mg) of shows significant growth inhibition with an LD50 of 30 µM against the tropical parasite Leishmania major, which causes severe clinical manifestations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coumarin; Aurapten (7-geranyloxycoumarin); X-rays; Leishmania; Inhibitor.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200010

In vivo and in vitro effect of imipramine and fluoxetine on Na+,K+-ATPase activity in synaptic plasma membranes from the cerebral cortex of rats
Provedor de dados: 56
Autores: Zanatta,L.M.; Nascimento,F.C.; Barros,S.V.T.; Silva,G.R.R.S.; Zugno,A.I.; Netto,C.A.; Wyse,A.T.S.
The effects of in vivo chronic treatment and in vitro addition of imipramine, a tricyclic antidepressant, or fluoxetine, a selective serotonin reuptake inhibitor, on the cortical membrane-bound Na+,K+-ATPase activity were studied. Adult Wistar rats received daily intraperitoneal injections of 10 mg/kg of imipramine or fluoxetine for 14 days. Twelve hours after the last injection rats were decapitated and synaptic plasma membranes (SPM) from cerebral cortex were prepared to determine Na+,K+-ATPase activity. There was a significant decrease (10%) in enzyme activity after imipramine but fluoxetine treatment caused a significant increase (27%) in Na+,K+-ATPase activity compared to control (P&lt;0.05, ANOVA; N = 7 for each group). When assayed in vitro, the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Imipramine fluoxetine Na+; K+-ATPase cerebral cortex.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000005

A low concentration of ouabain (0.18 µg/kg) enhances hypertension in spontaneously hypertensive rats by inhibiting the Na+ pump and activating the renin-angiotensin system
Provedor de dados: 56
Autores: Siman,F.D.M.; Stefanon,I.; Vassallo,D.V.; Padilha,A.S.
We investigated the effects of low ouabain concentrations on systolic (SAP) and diastolic (DAP) arterial pressures and on pressor reactivity in 3-month-old male spontaneously hypertensive rats (SHR). Arterial blood pressure (BP) and pressor reactivity to phenylephrine (PHE) were investigated before and after 0.18 μg/kg ouabain administration (N = 6). The influence of hexamethonium (N = 6), canrenone (N = 6), enalapril (N = 6), and losartan (N = 6) on ouabain actions was evaluated. Ouabain increased BP (SAP: 137 ± 5.1 to 150 ± 4.7; DAP: 93.7 ± 7.7 to 116 ± 3.5 mmHg; P&lt;0.05) but did not change PHE pressor reactivity. Hexamethonium reduced basal BP in control but not in ouabain-treated rats. However, hexamethonium + ouabain increased DAP sensitivity to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ouabain; Hypertension; Na+ pump; Renin-angiotensin system.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800010

Ethnicity as a risk factor for obstructive sleep apnea: comparison of Japanese descendants and white males in São Paulo, Brazil
Provedor de dados: 56
Autores: Genta,P.R.; Marcondes,B.F.; Danzi,N.J.; Lorenzi-Filho,G.
Some studies showed that Asians with obstructive sleep apnea (OSA) are thinner than Caucasians. Because obesity is a major risk factor for OSA, it was concluded that Asians are predisposed to OSA. However, body fat composition varies for a same body mass index (BMI) according to ethnicity. We firstly compared anthropometric characteristics, symptoms and associated disorders in all consecutive male Japanese descendants and white males with OSA referred for polysomnography. In a second analysis, all Japanese descendants were compared to a subgroup of white males, matched for apnea/hypopnea index and age. In the first analysis, age, symptoms, OSA severity and co-morbidities were similar among Japanese descendants (N = 54) and white patients (N = 466)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asians; Obesity; Obstructive sleep apnea; Japanese; Ethnicity; Body mass index.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800015

Physical exercise prevents motor disorders and striatal oxidative imbalance after cerebral ischemia-reperfusion
Provedor de dados: 56
Autores: Sosa,P.M.; Schimidt,H.L.; Altermann,C.; Vieira,A.S.; Cibin,F.W.S.; Carpes,F.P.; Mello-Carpes,P.B.
Stroke is the third most common cause of death worldwide, and most stroke survivors present some functional impairment. We assessed the striatal oxidative balance and motor alterations resulting from stroke in a rat model to investigate the neuroprotective role of physical exercise. Forty male Wistar rats were assigned to 4 groups: a) control, b) ischemia, c) physical exercise, and d) physical exercise and ischemia. Physical exercise was conducted using a treadmill for 8 weeks. Ischemia-reperfusion surgery involved transient bilateral occlusion of the common carotid arteries for 30 min. Neuromotor performance (open-field and rotarod performance tests) and pain sensitivity were evaluated beginning at 24 h after the surgery. Rats were euthanized and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stroke; Striatum; Locomotion; Oxidative stress; Antioxidants; Running.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900798

Expression of c-erbB-2, p53 and c-myc proteins in male breast carcinoma: Comparison with traditional prognostic factors and survival
Provedor de dados: 56
Autores: Mourão Netto,M.; Logullo,A.F.; Nonogaki,S.; Brentani,R.R.; Brentani,M.M.
There are few data evaluating biological markers for men with breast cancer. The purpose of the present study was to analyze the expression of the oncogenes c-erbB-2 and c-myc and of the suppressor gene p53 by immunohistochemical techniques in archival paraffin-embedded tissue blocks of 48 male breast cancer patients, treated at the A.C. Camargo Cancer Hospital, São Paulo, SP, Brazil. The results were compared with clinicopathological prognostic features. Immunopositivity of c-erbB-2, p53 and c-myc was detected in 62.5, 16.7 and 20.8% of the cases analyzed, respectively. Estrogen and progesterone receptors were positive in 75 and 69% of the cases, respectively. Increasing staging was statistically associated with c-erbB-2 (P = 0.04) and weakly related to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P53; C-myc; C-erbB-2; Male breast cancer.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700008

Actions of Crotalus durissus terrificus venom and crotoxin on the isolated rat kidney
Provedor de dados: 56
Autores: Monteiro,H.S.A.; Silva,I.M.S.C. da; Martins,A.M.C.; Fonteles,M.C.
Many studies have reported the occurrence of lethal acute renal failure after snakebites. The aim of the present investigation was to determine alterations in renal function produced by Crotalus durissus terrificus venom and crotoxin as well as the histological alterations induced by these venoms. Isolated kidneys from Wistar rats weighing 240 to 280 g were perfused with Krebs-Henseleit solution containing 6 g% of previously dialyzed bovine serum albumin. The effects of Crotalus durissus terrificus venom and crotoxin were studied on glomerular filtration rate (GFR), urinary flow (UF), perfusion pressure (PP) and percentage sodium tubular transport (%TNa+). The infusion of Crotalus durissus terrificus venom (10 µg/ml) and crotoxin (10 µg/ml) increased GFR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus venom; Crotoxin; Kidney.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000017

Organic solvent exposure and contrast sensitivity: comparing men and women
Provedor de dados: 56
Autores: Oliveira,A.R.; Campos Neto,A.A.; de Andrade,M.J.O.; de Medeiros,P.C.B.; dos Santos,N.A.
The goal of this study was to compare the visual contrast sensitivity (CS) of men and women exposed and not exposed to organic solvents. Forty-six volunteers of both genders aged between 18 and 41 years (mean±SD=27.72±6.28) participated. Gas station attendants were exposed to gas containing 46.30 ppm of solvents at a temperature of 304±274.39 K, humidity of 62.25±7.59% and ventilation of 0.69±0.46 m/s (a passive gas chromatography-based sampling method was used considering the microclimate variables). Visual CS was measured via the psychophysical method of two-alternative forced choice using vertical sinusoidal gratings with spatial frequencies of 0.2, 0.5, 1.0, 2.0, 5.0, 10.0, and 16.0 cpd (cycles per degree) and an average luminance of 34.4 cd/m2. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visual perception; Spatial frequencies; Neurotoxic agents; Chronic exposure; Gender.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300603

Reduction of the amount of intestinal secretory IgA in fulminant hepatic failure
Provedor de dados: 56
Autores: Dong-yan,Liu; Weiguo,Jiang; Pei,Liu.
Intestinal barrier dysfunction plays an important role in spontaneous bacterial peritonitis. In the present study, changes in the intestinal barrier with regard to levels of secretory immunoglobulin A (SIgA) and its components were studied in fulminant hepatic failure (FHF). Immunohistochemistry and double immunofluorescent staining were used to detect intestinal IgA, the secretory component (SC) and SIgA in patients with FHF (20 patients) and in an animal model with FHF (120 mice). Real-time PCR was used to detect intestinal SC mRNA in the animal model with FHF. Intestinal SIgA, IgA, and SC staining in patients with FHF was significantly weaker than in the normal control group (30 patients). Intestinal IgA and SC staining was significantly weaker in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fulminant hepatic failure; Secretory IgA; IgA; Secretory component; Intestine.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500014

Polydatin protects H9c2 cells from hypoxia-induced injury via up-regulating long non-coding RNA DGCR5
Provedor de dados: 56
Autores: Dai,Jinhua; Ma,Jianbo; Liao,Yufeng; Luo,Xianhai; Chen,Guofang.
Polydatin (PD), a monocrystalline polyphenolic drug mainly found in the roots of Polygonum cuspidatum, has various pharmacological activities. Long non-coding RNAs (lncRNA) DiGeorge syndrome critical region gene 5 (DGCR5) was found to participate in the suppression of multiple cancers. Here, we proposed to study the effect of PD on myocardial infarction (MI) by inducing DGCR5. CCK-8 assay was performed to detect the viability of H9c2 cells. Flow cytometry was utilized to test apoptosis of H9c2 cells. These results determined the optimal concentration and effect time of hypoxia as well as PD. Si-DGCR5 was transfected into cells and the expression level was determined by qRT-PCR. Western blot was utilized to evaluate the expression of apoptosis-related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial infarction; Apoptosis; Autophagy; PI3K/AKT/mTOR pathway; MEK/ERK pathway.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200613

Pediatric pain: prevalence, assessment, and management in a teaching hospital
Provedor de dados: 56
Autores: Linhares,M.B.M.; Doca,F.N.P.; Martinez,F.E.; Carlotti,A.P.P.; Cassiano,R.G.M.; Pfeifer,L.I.; Funayama,C.A.; Rossi,L.R.G.; Finley,G.A.
The goal of this study was to examine the prevalence, assessment and management of pediatric pain in a public teaching hospital. The study sample consisted of 121 inpatients (70 infants, 36 children, and 15 adolescents), their families, 40 physicians, and 43 nurses. All participants were interviewed except infants and children who could not communicate due to their clinical status. The interview included open-ended questions concerning the inpatients’ pain symptoms during the 24 h preceding data collection, as well as pain assessment and pharmacological/non-pharmacological management of pain. The data were obtained from 100% of the eligible inpatients. Thirty-four children/adolescents (28%) answered the questionnaire and for the other 72% (unable to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pain; Inpatients; Policy; Clinical audit.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200026

Dialysis water treated by reverse osmosis decreases the levels of C-reactive protein in uremic patients
Provedor de dados: 56
Autores: Thomé,F.S.; Senger,M.; Garcez,C.; Garcez,J.; Chemello,C.; Manfro,R.C.
Atherosclerosis is a major complication of chronic renal failure. Microinflammation is involved in atherogenesis and is associated with uremia and dialysis. The role of dialysate water contamination in inducing inflammation has been debated. Our aim was to study inflammatory markers in patients on chronic dialysis, before and 3 to 6 months after switching the water purification system from deionization to reverse osmosis. Patients had demographic, clinical and nutritional information collected and blood drawn for determination of albumin, ferritin, C-reactive protein (CRP), interleukin-6, and tumor necrosis factor-alpha in both situations. Acceptable levels of water purity were less than 200 colony-forming units of bacteria and less than 1 ng/ml of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemodialysis; C-reactive protein; Dialysate water; Reverse osmosi; Inflammation; Uremia.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500018

Coronary endothelial dysfunction after ischemia and reperfusion: a new therapeutic target?
Provedor de dados: 56
Autores: Laude,K.; Thuillez,C.; Richard,V.
Although cardiac ischemia is usually characterized as a disease of the myocyte, it is clear that the vasculature, and especially endothelial cells, is also a major target of this pathology. Indeed, using a rat model of ischemia/reperfusion, we were able to detect severe endothelial dysfunction (assessed as a decreased response to acetylcholine) after acute or chronic reperfusion. Given the essential role of the endothelium in the regulation of vascular tone, as well as platelet and leukocyte function, such a severe dysfunction could lead to an increased risk of vasospasm, thrombosis and accelerated atherosclerosis. This dysfunction can be prevented by free radical scavengers and by exogenous nitric oxide. Endothelial dysfunction can also be prevented by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary endothelium; Ischemia/reperfusion; Preconditioning.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100001

Cardiovascular complications and increased levels of circulating modified low density lipoprotein in HIV patients and patients with lipodystrophy
Provedor de dados: 56
Autores: Ronchini,K.R.O.M.; Duarte,A.J.S.; Casseb,J.S.R.; Gidlund,M.
The introduction of highly active antiretroviral therapy (HAART) for patients infected with HIV has significantly prolonged the life expectancy and to some extent has restored a functional immune response. However, the premature introduction of HAART has led to a significant and alarming increase in cardiovascular complications, including myocardial infarction and the appearance of abnormal distribution of body fat seen as lipodystrophy. One key element in the development of ischemic coronary artery disease is the presence of circulating and tissue-fixed modified low density lipoprotein (mLDL) that contributes to the initiation and progression of arterial lesions and to the formation of foam cells. Even though not completely elucidated, the most likely...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HIV; Oxidized LDL; Modified low density lipoprotein; Lipodystrophy; Coronary artery disease.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100016

Influence of eNOS gene polymorphism on cardiometabolic parameters in response to physical training in postmenopausal women
Provedor de dados: 56
Autores: Esposti,R.D.; Sponton,C.H.G.; Malagrino,P.A.; Carvalho,F.C.; Peres,E.; Puga,G.M.; Novais,I.P.; Albuquerque,D.M.; Rodovalho,C.; Bacci,M.; Zanesco,A.
The health-promoting effects of exercise training (ET) are related to nitric oxide (NO) production and/or its bioavailability. The objective of this study was to determine whether single nucleotide polymorphism of the endothelial NO synthase (eNOS) gene at positions -786T&gt;C, G894T (Glu298Asp) and at the variable number of tandem repeat (VNTR) Intron 4b/a would interfere with the cardiometabolic responses of postmenopausal women submitted to physical training. Forty-nine postmenopausal women were trained in sessions of 30-40 min, 3 days a week for 8 weeks. Genotypes, oxidative stress status and cardiometabolic parameters were then evaluated in a double-blind design. Both systolic and diastolic blood pressure values were significantly reduced after ET,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise training; ENOS polymorphism; Oxidative stress; Nitric oxide; Women.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900005

Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm
Provedor de dados: 56
Autores: Brito,C.F.A.; Oliveira,G.C.; Oliveira,S.C.; Street,M.; Riengrojpitak,S.; Wilson,R.A.; Simpson,A.J.G.; Correa-Oliveira,R.
Sm14 is a 14-kDa vaccine candidate antigen from Schistosoma mansoni that seems to be involved in cytoplasmic trafficking of fatty acids. Although schistosomes have a high requirement for lipids, they are not able to synthesize fatty acids and sterols de novo. Thus, they must acquire host lipids. In order to determine whether Sm14 is present in different stages of the life cycle of the parasite, we performed RT-PCR. Sm14 mRNA was identified in all stages of the life cycle studied, mainly schistosomulum, adult worm and egg. Additionally, we used a rabbit anti-Sm14 polyclonal antibody in an indirect immunofluorescence assay to localize Sm14 in adult worm sections. The basal lamella of the tegument and the gut epithelium were strongly labeled. These tissues...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sm14; Fatty acid-binding protein; FABP; Immunolocalization; RT-PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300014

An extract of Polygonum multiflorum protects against free radical damage induced by ultraviolet B irradiation of the skin
Provedor de dados: 56
Autores: Hwang,I.K.; Yoo,K.-Y.; Kim,D.W.; Jeong,S.J.; Won,C.-K.; Moon,W.-K.; Kim,Y.S.; Kwon,D.Y.; Won,M.H.; Kim,D.-W.
Over the last decades, the incidence of ultraviolet B (UVB)-related skin problems has been increasing. Damages induced by UVB radiation are related to mutations that occur as a result of direct DNA damage and/or the production of reactive oxygen species. We investigated the anti-oxidant effects of a Polygonum multiflorum thumb extract against skin damage induced by UVB irradiation. Female SKH-1 hairless mice were divided into three groups: control (N = 7), distilled water- (N = 10), and P. multiflorum extract-treated (PM, N = 10) groups. The PM (10 g) was extracted with 100 mL distilled water, cryo-dried and 9.8 g was obtained. The animals received a topical application of 500 µL distilled water or PM extract (1, 2, 4, 8, and 16%, w/v, dissolved in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polygonum multiflorum extract Skin Ultraviolet B irradiation Free radicals Cu; Zn-superoxide dismutase Hairless mouse.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900005

Erythropoietin reduces the expression of myostatin in mdx dystrophic mice
Provedor de dados: 56
Autores: Feder,D.; Rugollini,M.; Santomauro Jr,A.; Oliveira,L.P.; Lioi,V.P.; dos Santos,R.; Ferreira,L.G.; Nunes,M.T.; Carvalho,M.H.; Delgado,P.O.; Carvalho,A.A.S.; Fonseca,F.L.A.
Erythropoietin (EPO) has been well characterized as a renal glycoprotein hormone regulating red blood cell production by inhibiting apoptosis of erythrocyte progenitors in hematopoietic tissues. EPO exerts regulatory effects in cardiac and skeletal muscles. Duchenne muscular dystrophy is a lethal degenerative disorder of skeletal and cardiac muscle. In this study, we tested the possible therapeutic beneficial effect of recombinant EPO (rhEPO) in dystrophic muscles in mdx mice. Total strength was measured using a force transducer coupled to a computer. Gene expression for myostatin, transforming growth factor-β1 (TGF-β1), and tumor necrosis factor-α (TNF-α) was determined by quantitative real time polymerase chain reaction. Myostatin expression was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscular dystrophy; Erythropoietin; Myostatin; Skeletal muscle; Quadriceps.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100966

Correction
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/other
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600018

Ischemic preconditioning reduces peripheral oxidative damage associated with brain ischemia in rats
Provedor de dados: 56
Autores: Frassetto,S.S.; Schetinger,M.R.C.; Webber,A.; Sarkis,J.J.F.; Netto,C.A.
Brain ischemia followed by reperfusion causes neuronal death related to oxidative damage. Furthermore, it has been reported that subjects suffering from ischemic cerebrovascular disorders exhibit changes in circulating platelet aggregation, a characteristic that might be important for their clinical outcome. In the present investigation we studied tert-butyl hydroperoxide-initiated plasma chemiluminescence and thiol content as measures of peripheral oxidative damage in naive and preconditioned rats submitted to forebrain ischemia produced by the 4-vessel occlusion method. Rats were submitted to 2 or 10 min of global transient forebrain ischemia followed by 60 min or 1, 2, 5, 10 or 30 days of reperfusion. Preconditioned rats were submitted to a 10-min...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain ischemia; Ischemic preconditioning; Oxidative damage; Hydroperoxide-initiated chemiluminescence; Plasma thiols.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000017

Relationship between salt consumption measured by 24-h urine collection and blood pressure in the adult population of Vitória (Brazil)
Provedor de dados: 56
Autores: Rodrigues,S.L.; Souza Júnior,P.R.; Pimentel,E.B.; Baldo,M.P.; Malta,D.C.; Mill,J.G.; Szwarcwald,C.L.
High salt intake is related to an increase in blood pressure and development of hypertension. However, currently, there are no national representative data in Brazil using the gold standard method of 24-h urine collection to measure sodium consumption. This study aimed to determine salt intake based on 24-h urine collection in a sample of 272 adults of both genders and to correlate it with blood pressure levels. We used a rigorous protocol to assure an empty bladder prior to initiating urine collection. We excluded subjects with a urine volume &lt;500 mL, collection period outside of an interval of 23-25 h, and subjects with creatinine excretion that was not within the range of 14.4-33.6 mg/kg (men) and 10.8-25.2 mg/kg (women). The mean salt intake was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sodium; Salt intake; Blood pressure; Hypertension; 24-h urine collection; Population-based study.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800728

Cyclophosphamide in the treatment of focal segmental glomerulosclerosis
Provedor de dados: 56
Autores: Martinelli,R.; Pereira,L.J.; Silva,O.M.M.; Okumura,A.S.; Rocha,H.
Prednisone is the initial treatment of primary focal segmental glomerulosclerosis. However, when immunosuppressive agents in combination with steroids are used in the treatment of prednisone-dependent and prednisone-resistant patients the remission rate is variable. We report a long-term trial using cyclophosphamide (2.0 to 3.0 mg/kg body weight for 12 weeks) in combination with prednisone (1.0 to 2.0 mg/kg body weight), as compared with prednisone alone for the treatment of prednisone-resistant and frequently relapsing nephrotic syndrome and focal segmental glomerulosclerosis. Fifty-four patients (34 males and 20 females) with a diagnosis of idiopathic nephrotic syndrome and focal segmental glomerulosclerosis, followed-up for an average of 86.1 ± 82.4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclophosphamide; Prednisone; Nephrotic syndrome; Primary glomerular disease; Focal segmental glomerulosclerosis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900011

Hypertension is associated with greater heat exchange during exercise recovery in a hot environment
Provedor de dados: 56
Autores: Fonseca,S. F.; Teles,M. C.; Ribeiro,V. G. C.; Magalhães,F. C.; Mendonça,V. A.; Peixoto,M. F. D.; Leite,L. H. R.; Coimbra,C. C.; Lacerda,A. C. R.
Individuals with systemic arterial hypertension have a higher risk of heat-related complications. Thus, the aim of this study was to examine the thermoregulatory responses of hypertensive subjects during recovery from moderate-intensity exercise performed in the heat. A total of eight essential hypertensive (H) and eight normotensive (N) male subjects (age=46.5±1.3 and 45.6±1.4 years, body mass index=25.8±0.8 and 25.6±0.6 kg/m2, mean arterial pressure=98.0±2.8 and 86.0±2.3 mmHg, respectively) rested for 30 min, performed 1 h of treadmill exercise at 50% of maximal oxygen consumption, and rested for 1 h after exercise in an environmental chamber at 38°C and 60% relative humidity. Skin and core temperatures were measured to calculate heat exchange...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Hot Environment; Thermoregulation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201122

Cyclosporin A preferentially attenuates skeletal slow-twitch muscle regeneration
Provedor de dados: 56
Autores: Miyabara,E.H.; Aoki,M.S.; Moriscot,A.S.
Calcineurin, a Ca2+/calmodulin-dependent phosphatase, is associated with muscle regeneration via NFATc1/GATA2-dependent pathways. However, it is not clear whether calcineurin preferentially affects the regeneration of slow- or fast-twitch muscles. We investigated the effect of a calcineurin inhibitor, cyclosporin A (CsA), on the morphology and fiber diameter of regenerating slow- and fast-twitch muscles. Adult Wistar rats (259.5 ± 9 g) maintained under standard conditions were treated with CsA (20 mg/kg body weight, ip) for 5 days, submitted to cryolesion of soleus and tibialis anterior (TA) muscles on the 6th day, and then treated with CsA for an additional 21 days. The muscles were removed, weighed, frozen, and stored in liquid nitrogen. Cryolesion did...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Calcineurin; Cyclosporin A; Regeneration; Fast and slow twitch muscles.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400009

Phagocytosis by macrophages mediated by receptors for denatured proteins - dependence on tyrosine protein kinases
Provedor de dados: 56
Autores: Hespanhol,M.R.; Mantovani,B.
Previous studies have demonstrated that some components of the leukocyte cell membrane, CR3 (Mac-1, CD11b/CD18) and p150/95, are able to bind to denatured proteins. Thus, it is of interest to know which effector functions of these cells can be triggered by these receptors when they interact with particles or surfaces covered with denatured proteins. In the present study we analyzed their possible role as mediators of phagocytosis of red cells covered with denatured bovine serum albumin (BSA) by mouse peritoneal macrophages. We observed that a) macrophages are able to recognize (bind to) these red cells, b) this interaction can be inhibited by denatured BSA in the fluid phase, c) there is no phagocytosis of these particles by normal macrophages, d)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phagocytosis; Denatured proteins; Macrophage; SS2 Integrins; Tyrosine protein kinases.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300015

Drugs and lifestyle for the treatment and prevention of coronary artery disease: comparative analysis of the scientific basis
Provedor de dados: 56
Autores: da Luz,P.L.; Nishiyama,M.; Chagas,A.C.P.
In this article, we compare two strategies for atherosclerosis treatment: drugs and healthy lifestyle. Statins are the principal drugs used for the treatment of atherosclerosis. Several secondary prevention studies have demonstrated that statins can significantly reduce cardiovascular events including coronary death, the need for surgical revascularization, stroke, total mortality, as well as fatal and non-fatal myocardial infarction. These results were observed in both men and women, the elderly, smokers and non-smokers, diabetics and hypertensives. Primary prevention studies yielded similar results, although total mortality was not affected. Statins also induce atheroma regression and do not cause cancer. However, many unresolved issues remain, such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Statins; Lifestyle; Coronary disease; Prevention.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000002

Etanercept protects rat cardiomyocytes against hypertrophy by regulating inflammatory cytokines secretion and cell apoptosis
Provedor de dados: 56
Autores: Li,Q.; Yu,Q.; Na,R.; Liu,B.
We aimed to investigate the effect of etanercept, a tumor necrosis factor-α (TNF-α) inhibitor, on rat cardiomyocyte hypertrophy and its underlying mechanism. Primary neonatal rat cardiomyocytes were isolated from Sprague-Dawley rats. The model of rat cardiomyocyte hypertrophy was induced by endothelin, and then treated with different concentrations of etanercept (1, 10, and 50 μM). After treatment, cell counts, viability and cell apoptosis were evaluated. The mRNA levels of myocardial hypertrophy marker genes, including atrial natriuretic factor (ANF), matrix metalloproteinase (MMP)-9 and MMP-13, were detected by qRT-PCR, and the expressions of apoptosis-related proteins (Bcl-2 and Bax) were measured by western blotting. The protein levels of transforming...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tumor necrosis factor-α inhibitor; Endothelin; Myocardial hypertrophy; Cell apoptosis; Inflammatory response.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600604

Role of endothelium in angiotensin II formation by the rat aorta and mesenteric arterial bed
Provedor de dados: 56
We investigated the angiotensin II (Ang II)-generating system by analyzing the vasoconstrictor effect of Ang II, angiotensin I (Ang I), and tetradecapeptide (TDP) renin substrate in the absence and presence of inhibitors of the renin-angiotensin system in isolated rat aortic rings and mesenteric arterial beds with and without functional endothelium. Ang II, Ang I, and TDP elicited a dose-dependent vasoconstrictor effect in both vascular preparations that was completely blocked by the Ang II receptor antagonist saralasin (50 nM). The angiotensin converting enzyme (ACE) inhibitor captopril (36 µM) completely inhibited the vasoconstrictor effect elicited by Ang I and TDP in aortic rings without affecting that of Ang II. In contrast, captopril (36 µM)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Angiotensin II; Angiotensin converting enzyme; Renin-angiotensin system; Endothelium; Blood vessels; Captopril.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500013

Comparison of the quantification of caffeine in human plasma by gas chromatography and ELISA
Provedor de dados: 56
Autores: Carregaro,A.B.; Woods,W.E.; Tobin,T.; Queiroz-Neto,A.
In the present study we evaluated the precision of the ELISA method to quantify caffeine in human plasma and compared the results with those obtained by gas chromatography. A total of 58 samples were analyzed by gas chromatography using a nitrogen-phosphorus detector and routine techniques. For the ELISA test, the samples were diluted to obtain a concentration corresponding to 50% of the absorbance of the standard curve. To determine whether the proximity between the I50 of the standard curve and that of the sample would bring about a more precise result, the samples were divided into three blocks according to the criterion of difference, in modulus, of the I50 of the standard curve and of the I50 of the sample. The samples were classified into three...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gas chromatography; ELISA; Caffeine; Plasma; Quantification.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600019

Monoassociation with Lactobacillus acidophilus UFV-H2b20 stimulates the immune defense mechanisms of germfree mice
Provedor de dados: 56
Autores: Neumann,E.; Oliveira,M.A.P.; Cabral,C.M.; Moura,L.N.; Nicoli,J.R.; Vieira,E.C.; Cara,D.C.; Podoprigora,G.I.; Vieira,L.Q.
Probiotics are formulations containing live microorganisms or microbial stimulants that have some beneficial influence on the maintenance of a balanced intestinal microbiota and on the resistance to infections. The search for probiotics to be used in prevention or treatment of enteric infections, as an alternative to antibiotic therapy, has gained significant impulse in the last few years. Several studies have demonstrated the beneficial effects of lactic acid bacteria in controlling infection by intestinal pathogens and in boosting the host's nonspecific immune response. Here, we studied the use of Lactobacillus acidophilus UFV-H2b20, a lactic acid bacterium isolated from a human newborn from Viçosa, Minas Gerais, Brazil, as a probiotic. A suspension...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus; Macrophages; Kupffer cells; Probiotics.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200009

Faster gastric emptying of a liquid meal in rats after hypothalamic dorsomedial nucleus lesion
Provedor de dados: 56
Autores: Denofre-Carvalho,S.; Collares,E.F.; Fernandes,G.A.
The effects of dorsomedial hypothalamic (DMH) nucleus lesion on body weight, plasma glucose levels, and the gastric emptying of a liquid meal were investigated in male Wistar rats (170-250 g). DMH lesions were produced stereotaxically by delivering a 2.0-mA current for 20 s through nichrome electrodes (0.3-mm tip exposure). In a second set of experiments, the DMH and the ventromedial hypothalamic (VMH) nucleus were lesioned with a 1.0-mA current for 10 s (0.1-mm tip exposure). The medial hypothalamus (MH) was also lesioned separately using a nichrome electrode (0.3-mm tip exposure) with a 2.0-mA current for 20 s. Gastric emptying was measured following the orogastric infusion of a liquid test meal consisting of physiological saline (0.9% NaCl, w/v) plus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Hypothalamus; Dorsomedial nucleus.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900011

Improved glycemic control by acarbose therapy in hypertensive diabetic patients: effects on blood pressure and hormonal parameters
Provedor de dados: 56
Autores: Rosenbaum,P.; Peres,R.B.; Zanella,M.T.; Ferreira,S.R.G.
A double-blind, randomized, placebo-controlled study was carried out on 44 hypertensive type 2 diabetic subjects previously treated by diet associated or not with sulfonylurea to assess the effects of acarbose-induced glycemic control on blood pressure (BP) and hormonal parameters. Before randomization and after a 22-week treatment period (100 to 300 mg/day), the subjects were submitted to a standard meal test and to 24-h ambulatory BP monitoring (ABPM) and had plasma glucose, glycosylated hemoglobin, lipid profile, insulin, proinsulin and leptin levels determined. Weight loss was found only in the acarbose-treated group (75.1 ± 11.6 to 73.1 ± 11.6 kg, P&lt;0.01). Glycosylated hemoglobin decreased only in the acarbose group (6.4 ± 1.7 to 5.6 ± 1.9%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes mellitus; Acarbose; Hypertension; Hyperinsulinemia.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800004

Hepatic inducible nitric oxide synthase expression increases upon exposure to hypergravity
Provedor de dados: 56
Autores: Kim,H.S.; Jung,Y.Y.; Do,S.I.
Stimulation by a number of conditions, including infection, cytokines, mechanical injury, and hypoxia, can upregulate inducible nitric oxide synthase (iNOS) in hepatocytes. We observed that exposure to hypergravity significantly upregulated the transcription of the hepatic iNOS gene. The aim of this study was to confirm our preliminary data, and to further investigate the distribution of the iNOS protein in the livers of mice exposed to hypergravity. ICR mice were exposed to +3 Gz for 1 h. We investigated the time course of change in the iNOS expression. Hepatic iNOS mRNA expression progressively increased in centrifuged mice from 0 to 12 h, and then decreased rapidly by 18 h. iNOS mRNA levels in the livers of centrifuged mice was significantly higher at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inducible nitric oxide synthase; Mouse; Liver; Hypergravity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100940

Effect of iodide on Fas, Fas-ligand and Bcl-w mRNA expression in thyroid of NOD mice pretreated with methimazole
Provedor de dados: 56
Autores: Boechat,L.H.B.; Vilella,C.A.; Zollner,R.L.
Nonobese diabetic (NOD) mice and a derived strain, NOD.H.2h4, have been used as a model for experimental spontaneous thyroiditis and thyroiditis induced by iodide excess after a goiter-inducing period. Some authors have proposed that iodide, given after methimazole or propylthiouracil, is capable of inducing apoptosis in thyroid cells and that anti-thyroid drugs can modulate the expression of apoptosis components such as Fas and its ligand (Fas-L). Here we evaluated the effect of potassium iodide (20 µg/animal for 4 days, ip) given to NOD mice at the 10th week of life after exposure to methimazole (1 mg/ml) in drinking water from the 4th to the 10th week of life. Fas, Fas-L and Bcl-w expression were analyzed semiquantitatively by RT-PCR immediately after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroiditis; Nonobese diabetic mice; Apoptosis; Methimazole; Iodide; Fas mRNA; Fas-L mRNA; Bcl-w mRNA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300003

Long-term effects of human amniotic membrane in a rat model of biliary fibrosis
Provedor de dados: 56
Autores: Sant'Anna,L.B.; Brito,F.S.; Barja,P.R.; Nicodemo,M.C.
Liver fibrosis is the most common outcome of chronic liver diseases, and its progression to cirrhosis can only be effectively treated with liver transplantation. The amniotic membrane (AM) has been studied as an alternative therapy for fibrosis diseases mainly for its favorable properties, including anti-inflammatory, anti-scaring and immunomodulatory properties. It was recently demonstrated that the AM reduces the progression of biliary fibrosis to its advanced stage, cirrhosis, when applied on the liver for 6 weeks after fibrosis induction. Here, we investigated the effects of AM on rat fibrotic liver, during a prolonged period of time. Fibrosis was induced by bile duct ligation (BDL), and at the same time, a fragment of AM was applied around the liver....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amniotic membrane; Bile duct ligation; Liver fibrosis; Long-term; Quantitative image analysis; Rat.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700611

Sequencing and promoter analysis of the nifENXorf3orf5fdxAnifQ operon from Azospirillum brasilense Sp7
Provedor de dados: 56
Autores: Potrich,D.P.; Bressel,T.A.; Schrank,I.S.; Passaglia,L.M.P.
A 40-kb DNA region containing the major cluster of nif genes has been isolated from the Azospirillum brasilense Sp7 genome. In this region three nif operons have been identified: nifHDKorf1Y, nifENXorf3orf5fdxAnifQ and orf2nifUSVorf4. The operons containing nifENX and nifUSV genes are separated from the structural nifHDKorf1Y operon by about 5 kb and 10 kb, respectively. The present study shows the sequence analysis of the 6045-bp DNA region containing the nifENX genes. The deduced amino acid sequences from the open reading frames were compared to the nif gene products of other diazotrophic bacteria and indicate the presence of seven ORFs, all reading in the same direction as that of the nifHDKorf1Y operon. Consensus sigma54 and NifA-binding sites are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Azospirillum brasilense; Nif genes; NifENX operon; Promoter activity.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100003

Upregulation of microRNA 142-3p in the peripheral blood and urinary cells of kidney transplant recipients with post-transplant graft dysfunction
Provedor de dados: 56
Autores: Domenico,T.D.; Joelsons,G.; Montenegro,R.M.; Manfro,R.C.
We analyzed microRNA (miR)-142-3p expression in leucocytes of the peripheral blood and urinary sediment cell samples obtained from kidney transplant recipients who developed graft dysfunction. Forty-one kidney transplant recipients with kidney graft dysfunction and 8 stable patients were included in the study. The groups were divided according to histological analysis into acute rejection group (n=23), acute tubular necrosis group (n=18) and stable patients group used as a control for gene expression (n=8). Percutaneous biopsies were performed and peripheral blood samples and urine samples were obtained. miR-142-3p was analyzed by real-time polymerase chain reaction. The group of patients with acute tubular necrosis presented significantly higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNA; Graft dysfunction; Renal transplantation; Gene expression; Biomarker.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400605

Plasma levels and placental expression of vaspin in pregnant women with diabetes mellitus
Provedor de dados: 56
Autores: Huo,Y.; Liu,S.X.; Song,G.Y.; Ren,L.P.; Wang,C.; Zhang,D.H.
The present study aimed to investigate visceral adipose tissue-specific serpin (vaspin) concentrations in serum and term placentas and relate these values to insulin resistance and lipid parameters in women with gestational diabetes mellitus (GDM). A total of 30 GDM subjects and 27 age-matched pregnant women with normal glucose tolerance (NGT, control) were included. Serum glucose, glycated hemoglobin (HbA1c), lipid profile, insulin, and vaspin were measured at the end of pregnancy, and homeostasis model of assessment-insulin resistance (HOMA-IR) values were calculated. Vaspin mRNA and protein levels in placentas were measured by real-time fluorescence quantitative reverse transcription polymerase chain reaction (RT-qPCR) and Western blotting,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaspin; Gestational diabetes; Placenta; Pregnancy; Insulin resistance.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300273

Differential effects of isoproterenol on the activity of angiotensin-converting enzyme in the rat heart and aorta
Provedor de dados: 56
Autores: Busatto,V.C.W.; Cunha,V.; Cicilini,M.A.; Mill,J.G.
The excessive stimulation of beta-adrenergic receptors in the heart induces myocardial hypertrophy. There are several experimental data suggesting that this hypertrophy may also depend, at least partially, on the increase of local production of angiotensin II secondary to the activation of the cardiac renin-angiotensin system. In this study we investigated the effects of isoproterenol on the activity of angiotensin-converting enzyme (ACE) in the heart and also in the aorta and plasma. Male Wistar rats weighing 250 to 305 g were treated with a dose of (±)-isoproterenol (0.3 mg kg-1 day-1, N = 8) sufficient to produce cardiac hypertrophy without deleterious effects on the pumping capacity of the heart. Control rats (N = 7) were treated with vehicle (corn...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Angiotensin-converting enzyme; Isoproterenol; Cardiac hypertrophy; Myocardial infarction; Heart failure.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300017

Crotoxin, the major toxin from the rattlesnake Crotalus durissus terrificus, inhibits ³H-choline uptake in guinea pig ileum
Provedor de dados: 56
Autores: Kattah,L.S.; Santoro,M.M.; Diniz,C.R.; De Lima,M.E.
We examined the effect of crotoxin, the neurotoxic complex from the venom of the South American rattlesnake Crotalus durissus terrificus, on the uptake of ³H-choline in minces of smooth muscle myenteric plexus from guinea pig ileum. In the concentration range used (0.03-1 µM) and up to 10 min of treatment, crotoxin decreased ³H-choline uptake by 50-75% compared to control. This inhibition was time dependent and did not seem to be associated with the disruption of the neuronal membrane, because at least for the first 20 min of tissue exposure to the toxin (up to 1 µM) the levels of lactate dehydrogenase (LDH) released into the supernatant were similar to those of controls. Higher concentrations of crotoxin or more extensive incubation times with this toxin...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Crotoxin; Choline uptake; Guinea pig ileum; Crotalus durissus terrificus; Phospholipase A2.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900017

Dynamic resistance training decreases sympathetic tone in hypertensive ovariectomized rats
Provedor de dados: 56
Autores: Shimojo,G.L.; Palma,R.K.; Brito,J.O.; Sanches,I.C.; Irigoyen,M.C.; De Angelis,K.
The aim of this study was to investigate the effects of resistance exercise training on hemodynamics and cardiac autonomic control in ovariectomized spontaneously hypertensive rats. Female rats were divided into 4 groups: sedentary control (SC), sedentary hypertensive (SH), sedentary hypertensive ovariectomized (SHO), and resistance-trained hypertensive ovariectomized (RTHO). Resistance exercise training was performed on a vertical ladder (5 days/week, 8 weeks) at 40-60% maximal load. Direct arterial pressure was recorded. Vagal and sympathetic tones were measured by heart rate (HR) responses to methylatropine (3 mg/kg, iv) and propranolol (4 mg/kg, iv). Ovariectomy resulted in additional increases in blood pressure in hypertensive rats and was associated...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Resistance exercise training; Menopause; Hypertension; Sympathetic tone; Blood pressure.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600523

Supraorganized collagen enhances Schwann cell reactivity and organization in vitro
Provedor de dados: 56
Autores: Maturana,L.G.; Zanon,R.G.; Pierucci,A.; Vidal,B.C.; Oliveira,A.L.R.
We investigated the reactivity and expression of basal lamina collagen by Schwann cells (SCs) cultivated on a supraorganized bovine-derived collagen substrate. SC cultures were obtained from sciatic nerves of neonatal Sprague-Dawley rats and seeded on 24-well culture plates containing collagen substrate. The homogeneity of the cultures was evaluated with an SC marker antibody (anti-S-100). After 1 week, the cultures were fixed and processed for immunocytochemistry by using antibodies against type IV collagen, S-100 and p75NTR (pan neurotrophin receptor) and for scanning electron microscopy (SEM). Positive labeling with antibodies to the cited molecules was observed, indicating that the collagen substrate stimulates SC alignment and adhesion (collagen IV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: S-100; P75NTR; Type IV collagen; Collagen; Cell culture; Supraorganized collagen.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700011

Effect of carbohydrate availability on time to exhaustion in exercise performed at two different intensities
Provedor de dados: 56
Autores: Lima-Silva,A.E.; De-Oliveira,F.R.; Nakamura,F.Y.; Gevaerd,M.S.
This study examined the effects of pre-exercise carbohydrate availability on the time to exhaustion for moderate and heavy exercise. Seven men participated in a randomized order in two diet and exercise regimens each lasting 3 days with a 1-week interval for washout. The tests were performed at 50% of the difference between the first (LT1) and second (LT2) lactate breakpoint for moderate exercise (below LT2) and at 25% of the difference between the maximal load and LT2 for heavy exercise (above LT2) until exhaustion. Forty-eight hours before each experimental session, subjects performed a 90-min cycling exercise followed by 5-min rest periods and a subsequent 1-min cycling bout at 125% VO2max/1-min rest periods until exhaustion to deplete muscle glycogen....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Time to exhaustion; Muscle glycogen; Metabolism; Lactate thresholds.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500002

Intrinsic bent DNA sites in the chromosomal replication origin of Xylella fastidiosa 9a5c
Provedor de dados: 56
Autores: Gimenes,F.; Gouveia,F. de S.; Fiorini,A.; Fernandez,M.A.
The features of the nucleotide sequences in both replication and promoter regions have been investigated in many organisms. Intrinsically bent DNA sites associated with transcription have been described in several prokaryotic organisms. The aim of the present study was to investigate intrinsic bent DNA sites in the segment that holds the chromosomal replication origin, oriC, of Xylella fastidiosa 9a5c. Electrophoretic behavior analyses, as well as in silico analyses of both the 2-D projection and helical parameters, were performed. The chromosomal segment analyzed contains the initial sequence of the rpmH gene, an intergenic region, the dnaA gene, the oriC sequence, and the 5' partial sequence of the dnaN gene. The analysis revealed fragments with reduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intrinsic bent DNA; Prokaryotic replication origin; OriC; Xylella fastidiosa.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400007

Short-lasting systemic and regional benefits of early crystalloid infusion after intravenous inoculation of dogs with live Escherichia coli
Provedor de dados: 56
Autores: Garrido,A.G.; Poli de Figueiredo,L.F.; Cruz Jr.,R.J.; Silva,E.; Rocha e Silva,M.
We investigated the systemic and regional hemodynamic effects of early crystalloid infusion in an experimental model of septic shock induced by intravenous inoculation with live Escherichia coli. Anesthetized dogs received an intravenous infusion of 1.2 x 10(10) cfu/kg live E. coli in 30 min. After 30 min of observation, they were randomized to controls (no fluids; N = 7), or fluid resuscitation with lactated Ringer's solution, 16 ml/kg (N = 7) or 32 ml/kg (N = 7) over 30 min and followed for 120 min. Cardiac index, portal blood flow, mean arterial pressure, systemic and regional oxygen-derived variables, blood lactate, and gastric PCO2 were assessed. Rapid and progressive cardiovascular deterioration with reduction in cardiac output, mean arterial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac output; Escherichia coli; Gas tonometry; Portal blood flow; Septic shock.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600009

Inspiratory flow-volume curve in snoring patients with and without obstructive sleep apnea
Provedor de dados: 56
Autores: Amado,V.M.; Costa,A.C.G.A.; Guiot,M.; Viegas,C.A.; Tavares,P.
We analyzed the flow-volume curves of 50 patients with complaints of snoring and daytime sleepiness in treatment at the Pneumology Unit of the University Hospital of Brasília. The total group was divided into snorers without obstructive sleep apnea (OSA) (N = 19) and snorers with OSA (N = 31); the patients with OSA were subdivided into two groups according to the apnea/hypopnea index (AHI): AHI&amp;lt;20/h (N = 14) and AHI&amp;gt;20/h (N = 17). The control group (N = 10) consisted of nonsmoking subjects without complaints of snoring, daytime sleepiness or pulmonary diseases. The population studied (control and patients) consisted of males of similar age, height and body mass index (BMI); spirometric data were also similar in the four groups. There was no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep; Flow-volume curve; Obstructive sleep apnea; Snoring; Upper airway resistance.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400005

Gene trio signatures as molecular markers to predict response to doxorubicin cyclophosphamide neoadjuvant chemotherapy in breast cancerpatients
Provedor de dados: 56
Autores: Katayama,M.L.H.; Brentani,H.; Abreu,A.P.S.; Barbosa,E.M.; Oliveira,C.T.; Góes,J.C.S.; Brentani,M.M.; Folgueira,M.A.A.K.
In breast cancer patients submitted to neoadjuvant chemotherapy (4 cycles of doxorubicin and cyclophosphamide, AC), expression of groups of three genes (gene trio signatures) could distinguish responsive from non-responsive tumors, as demonstrated by cDNA microarray profiling in a previous study by our group. In the current study, we determined if the expression of the same genes would retain the predictive strength, when analyzed by a more accessible technique (real-time RT-PCR). We evaluated 28 samples already analyzed by cDNA microarray, as a technical validation procedure, and 14 tumors, as an independent biological validation set. All patients received neoadjuvant chemotherapy (4 AC). Among five trio combinations previously identified, defined by nine...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Breast neoplasms; Discriminant analysis; Doxorubicin; Drug resistance; Neoadjuvant therapy; Reverse transcriptase-polymerase chain reaction.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200012

Effect of topical dorzolamide on rabbit central corneal thickness
Provedor de dados: 56
Autores: Almeida Jr.,G.C.; Faria e Souza,S.J.
Our objective was to study the effect of dorzolamide on corneal hydration in an 18-week controlled experiment using ultrasonic pachymetry. Twenty-eight male rabbits were divided randomly into four groups. The 7 rabbits in each group received eye drops containing either 2% (w/v) dorzolamide or placebo in their right eye, or in their left eye. The 2% dorzolamide rabbits were treated every 8 h. Fellow eyes are defined as eyes which did not receive either dorzolamide or placebo. The study was blind for both the person who applied the drug and the one who performed the pachymetry. The effect of treatments is reported on the basis of the percentage of pachymetric variation compared to the measurement made before drug application. There was no significant...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dorzolamide; Corneal thickness; Carbonic anhydrase inhibition; Ultrasonic pachymetry.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200015

An electronic pressure-meter nociception paw test for rats
Provedor de dados: 56
Autores: Vivancos,G.G.; Verri Jr.,W.A.; Cunha,T.M.; Schivo,I.R.S.; Parada,C.A.; Cunha,F.Q.; Ferreira,S.H.
The objective of the present investigation was to compare the sensitivity of an electronic nociceptive mechanical paw test with classical mechanical tests to quantify the intensity variation of inflammatory nociception. The electronic pressure-meter test consists of inducing the hindpaw flexion reflex by poking the plantar region with a polypropylene pipette tip adapted to a hand-held force transducer. This method was compared with the classical von Frey filaments test and with the rat paw constant pressure test, a modification of the Randall and Selitto test developed by our group. When comparing the three methods, the electronic pressure-meter and the rat paw constant pressure test, but not the von Frey filaments test, detected time vs treatment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperalgesia; Nociception; Dipyrone; Indomethacin; Von Frey filaments; Constant pressure test.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300017

Circulating endothelial cells are increased in chronic myeloid leukemia blast crisis
Provedor de dados: 56
Autores: Godoy,C.R.T.; Levy,D.; Giampaoli,V.; Chamone,D.A.F.; Bydlowski,S.P.; Pereira,J.
We measured circulating endothelial precursor cells (EPCs), activated circulating endothelial cells (aCECs), and mature circulating endothelial cells (mCECs) using four-color multiparametric flow cytometry in the peripheral blood of 84 chronic myeloid leukemia (CML) patients and 65 healthy controls; and vascular endothelial growth factor (VEGF) by quantitative real-time PCR in 50 CML patients and 32 healthy controls. Because of an increase in mCECs, the median percentage of CECs in CML blast crisis (0.0146%) was significantly higher than in healthy subjects (0.0059%, P&lt;0.01) and in the accelerated phase (0.0059%, P=0.01). There were no significant differences in the percentages of CECs in chronic- or active-phase patients and healthy subjects...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circulating endothelial cells; Vascular endothelial growth factor; Chronic myeloid leukemia; Flow cytometry; Angiogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600509

Effect of Camellia sinensis teas on left ventricular hypertrophy and insulin resistance in dyslipidemic mice
Provedor de dados: 56
Autores: Ferreira,M.C.L.; Lima,L.N.; Cota,L.H.T.; Costa,M.B.; Orsi,P.M.E.; Espíndola,R.P.; Albanez,A.V.; Rosa,B.B.; Carvalho,M.G.S.; Garcia,J.A.D.
The control of dyslipidemia using plants is an important subject of studies since it has numerous benefits in cardiovascular protection. The objective of this study was to evaluate the effect of three Camellia sinensis L. teas (green, red, and white) on left ventricular hypertrophy and insulin resistance in low-density lipoprotein receptor knockout (LDLr-/-) mice fed a high-fat diet. The LDLr-/- mice were divided into four experimental groups: Group C: standard feed; Group CT: standard feed and three teas, Group HL: high-fat feed; HLT Group: high-fat feed and three teas. The three types of tea (green, red, and white) originated from different processing of the Camellia sinensis L. plant, and were administered associated once a day at a dose of 25 mg/kg by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipids; Cardiovascular system; Tea; Glucose; Insulin.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500607

Skp2 inhibitor SKPin C1 decreased viability and proliferation of multiple myeloma cells and induced apoptosis
Provedor de dados: 56
Autores: Yang,Ying; Yan,Wei; Liu,Zhuogang; Wei,Minjie.
Multiple myeloma (MM) is a malignant neoplasm of plasma, and exhibits several harmful effects including osteolytic injuries, hypercalcemia, and immune dysfunction. Many patients with MM succumb to the underlying malignancy. An S-phase kinase-related protein 2 (Skp2) inhibitor, designated SKPin C1, has been developed and confirmed to have an inhibitory effect on metastatic melanoma cells. This study aimed to determine the effect of SKPin C1 on MM. Normal B lymphocytes, THP-1 cells, and MM U266 and RPMI 8226 cells were exposed to various dosages of SKPin C1 for 48 h. Cell proliferation was determined by MTT, EdU staining, and cell cycle assays. Western blot assays were performed to assess intracellular protein levels of Skp2, p27, and cleaved caspase-3. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Skp2; SKPin C1; Multiple myeloma; P27; Ubiquitination.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500605

Angiotensin and baroreflex control of the circulation
Provedor de dados: 56
Autores: Head,G.A.; Saigusa,T.; Mayorov,D.N.
There is a close association between the location of angiotensin (Ang) receptors and many important brain nuclei involved in the regulation of the cardiovascular system. The present review encompasses the physiological role of Ang II in the brainstem, particularly in relation to its influence on baroreflex control of the heart and kidney. Activation of AT1 receptors in the brainstem by fourth ventricle (4V) administration to conscious rabbits or local administration of Ang II into the rostral ventrolateral medulla (RVLM) of anesthetized rabbits acutely increases renal sympathetic nerve activity (RSNA) and RSNA baroreflex responses. Administration of the Ang antagonist Sarile into the RVLM of anesthetized rabbits blocked the effects of Ang II on the RSNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; AT1 receptors; Losartan; Renal sympathetic activity; Baroreflex; Rostral ventrolateral medulla; Blood pressure; Rabbit.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900005

Exogenous normal lymph alleviates microcirculation disturbances and abnormal hemorheological properties in rats with disseminated intravascular coagulation
Provedor de dados: 56
Autores: Niu,Chun-Yu; Zhao,Zi-Gang; Zhang,Yu-Ping; Hou,Ya-Li; Li,Jun-Jie; Jiang,Hua; Zhang,Jing.
Disturbances of the microcirculation and abnormal hemorheological properties are important factors that play an important role in disseminated intravascular coagulation (DIC) and result in organ dysfunction or failure. In the present study, we established an animal model of DIC using intravenous Dextran 500 in rats, and used exogenous normal lymph corresponding to 1/15 of whole blood volume for injection through the left jugular vein. We found that normal lymph could improve the blood pressure and survival time of rats with DIC. The results regarding the mesenteric microcirculation showed that the abnormality of the diameter of mesenteric microvessels and micro-blood flow speed in the DIC+lymph group was significantly less than in the DIC+saline group....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disseminated intravascular coagulation; Lymph; Mesenteric microcirculation; Hemorheology; Dextran 500.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200138

Regulation of transgene expression in genetic immunization
Provedor de dados: 56
Autores: Harms,J.S.; Oliveira,S.C.; Splitter,G.A.
The use of mammalian gene expression vectors has become increasingly important for genetic immunization and gene therapy as well as basic research. Essential for the success of these vectors in genetic immunization is the proper choice of a promoter linked to the antigen of interest. Many genetic immunization vectors use promoter elements from pathogenic viruses including SV40 and CMV. Lymphokines produced by the immune response to proteins expressed by these vectors could inhibit further transcription initiation by viral promoters. Our objective was to determine the effect of IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt; on transgene expression driven by viral SV40 or CMV promoter/enhancer and the mammalian promoter/enhancer for the major...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA vaccine; Gene therapy; Regulation; Transcription; Transgene.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200003

Effects of the conditioned medium of mesenchymal stem cells on mouse oocyte activation and development
Provedor de dados: 56
Autores: Feng,D.Q.; Zhou,Y.; Ling,B.; Gao,T.; Shi,Y.Y.; Wei,H.M.; Tian,Z.G.
Mesenchymal stem cells (MSCs) have been reported to secrete a variety of cytokines and growth factors acting as trophic suppliers, but little is known regarding the effects of conditioned medium (CM) of MSCs isolated from femurs and tibias of mouse on the artificial activation of mouse oocytes and on the developmental competence of the parthenotes. In the current study, we investigated the effect of CM on the events of mouse oocyte activation, namely oscillations of cytosolic calcium concentration ([Ca²+]i), meiosis resumption, pronucleus formation, and parthenogenetic development. The surface markers of MSCs were identified with a fluorescence-activated cell sorter. The dynamic changes of the spindle and formation of pronuclei were examined by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem cells; Oocyte activation; Parthenogenesis; Calcium; Cytochalasin B.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600006

Bone mineral density and inflammatory bowel disease severity
Provedor de dados: 56
Autores: Lima,C.A.; Lyra,A.C.; Mendes,C.M.C.; Lopes,M.B.; Coqueiro,F.G.; Rocha,R.; Santana,G.O.
Inflammatory bowel disease (IBD) is associated with low bone mineral density (BMD). In this study, the association between disease severity and BMD in patients with IBD was evaluated. Associations between BMD and the Montreal classification, disease activity and drug therapy were also tested. A cross-sectional prevalence study with a comparison group was conducted. One hundred and twenty-eight patients were evaluated: 68 patients with ulcerative colitis (UC), and 60 with Crohn's disease (CD). The control group consisted of 67 healthy subjects. All patients and controls had BMD measured and in IBD patients, current medications, hospitalization, and disease location, extent and phenotype, according to the Montreal classification, were recorded. Multiple...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone mineral density; Ulcerative colitis; Crohn’s disease; Inflammatory bowel disease; Osteoporosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200614

Cardiopulmonary exercise testing variables as predictors of long-term outcome in thoracic sarcoidosis
Provedor de dados: 56
Autores: Lopes,A.J.; Menezes,S.L.S.; Dias,C.M.; Oliveira,J.F.; Mainenti,M.R.M.; Guimarães,F.S.
Cardiopulmonary exercise testing (CPET) plays an important role in the assessment of functional capacity in patients with interstitial lung disease. The aim of this study was to identify CPET measures that might be helpful in predicting the vital capacity and diffusion capacity outcomes of patients with thoracic sarcoidosis. A longitudinal study was conducted on 42 nonsmoking patients with thoracic sarcoidosis (median age = 46.5 years, 22 females). At the first evaluation, spirometry, the measurement of single-breath carbon monoxide diffusing capacity (D LCOsb) and CPET were performed. Five years later, the patients underwent a second evaluation consisting of spirometry and D LCOsb measurement. After 5 years, forced vital capacity (FVC)% and D LCOsb% had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sarcoidosis; Exercise; Respiratory function tests; Respiratory mechanics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300012

Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, Curcumae rhizoma and Sparganii rhizoma
Provedor de dados: 56
Autores: Yu,Cheng Hao; Zhao,Jin Shuang; Zhao,Hui; Peng,Teng; Shen,Dong Cheng; Xu,Qiu Xia; Li,Yao; Webb,R. Clinton; Wang,Mong Heng; Shi,Xing Ming; Peng,Cheng; Ding,Wei Jun.
The aim of this study was to elucidate the concise effects of a traditional herb pair, Curcumae rhizoma-Sparganii rhizoma (CRSR), on uterine leiomyoma (UL) by analyzing transcriptional profiling. The UL rat model was made by intramuscular injection of progesterone and gavage administration of diethylstilbestrol. From 11 weeks of the establishment of the model, rats of the UL+CRSR group were gavaged daily with CRSR (6.67 g/kg). The serum concentrations of progesterone (P) and estradiol (E2) were determined by radioimmunoassay, the uterine index was measured by caliper measurement, and the pathological status was observed by hematoxylin and eosin stain. Gene expression profiling was checked by NimbleGen Rat Gene Expression Microarrays. The results indicated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uterine leiomyoma; Transcriptional profiling; Extracellular matrix; Curcumae rhizoma; Sparganii rhizoma.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600606

Sleep disorders, sleepiness and traffic safety: a public health menace
Provedor de dados: 56
Autores: Pandi-Perumal,S.R.; Verster,J.C.; Kayumov,L.; Lowe,A.D.; Santana,M.G.; Pires,M.L.N.; Tufik,S.; Mello,M.T.
Sleep disorders are not uncommon and have been widely reported throughout the world. They have a profound impact on industrialized 24-h societies. Consequences of these problems include impaired social and recreational activities, increased human errors, loss of productivity, and elevated risk of accidents. Conditions such as acute and chronic insomnia, sleep loss, excessive sleepiness, shift-work, jet lag, narcolepsy, and sleep apnea warrant public health attention, since residual sleepiness during the day may affect performance of daily activities such as driving a car. Benzodiazepine hypnotics and zopiclone promote sleep, both having residual effects the following day including sleepiness and reduced alertness. In contrast, the non-benzodiazepine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep disorders; Sleepiness; Automobile and truck drivers; Automobile traffic; Public health.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700003

Techniques of radioautography for medical and biological research
Provedor de dados: 56
Autores: Nagata,T.
Standard techniques for radioautography used in biological and medical research can be classified into three categories, i.e., macroscopic radioautography, light microscopic radioautography and electron microscopic radioautography. The routine techniques used in these three procedures are described. With regard to macroscopic radioautography, whole body radioautography is a standard technique which employs freezing and cryosectioning and can demonstrate organ distributions of both soluble and insoluble compounds. In contrast, in light and electron microscopic radioautography, soluble and insoluble techniques are separated. In order to demonstrate insoluble labeled compounds, conventional chemical fixations such as formalin for light microscopy or buffered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macroscopic radioautography; Light microscopic radioautography; Electron microscopic radioautography; Methodology.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200002

Blastocyst-endometrium interaction: intertwining a cytokine network
Provedor de dados: 56
Autores: Castro-Rendón,W.A.; Castro-Álvarez,J.F.; Guzmán-Martinez,C.; Bueno-Sanchez,J.C.
The successful implantation of the blastocyst depends on adequate interactions between the embryo and the uterus. The development of the embryo begins with the fertilized ovum, a single totipotent cell which undergoes mitosis and gives rise to a multicellular structure named blastocyst. At the same time, increasing concentrations of ovarian steroid hormones initiate a complex signaling cascade that stimulates the differentiation of endometrial stromal cells to decidual cells, preparing the uterus to lodge the embryo. Studies in humans and in other mammals have shown that cytokines and growth factors are produced by the pre-implantation embryo and cells of the reproductive tract; however, the interactions between these factors that converge for successful...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo implantation; Trophoblast; Cytokines; Prostaglandins; Aspirin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100001

Aspirin plus calcium supplementation to prevent superimposed preeclampsia: a randomized trial
Provedor de dados: 56
Autores: Souza,E.V.; Torloni,M.R.; Atallah,A.N.; dos Santos,G.M.S.; Kulay Jr,L.; Sass,N.
Preeclampsia is an important cause of maternal and perinatal morbidity and mortality. Previous studies have tested calcium supplementation and aspirin separately to reduce the incidence of preeclampsia but not the effects of combined supplementation. The objective of this study was to investigate the effectiveness of aspirin combined with calcium supplementation to prevent preeclampsia in women with chronic hypertension. A double-blind, placebo-controlled randomized clinical trial was carried out at the antenatal clinic of a large university hospital in São Paulo, SP, Brazil. A total of 49 women with chronic hypertension and abnormal uterine artery Doppler at 20-27 weeks gestation were randomly assigned to receive placebo (N = 26) or 100 mg aspirin plus 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preeclampsia; Hypertension; Ultrasonography; Doppler; Calcium carbonate; Aspirin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500419

Ethanol-induced colitis prevents oral tolerance induction in mice
Provedor de dados: 56
Autores: Andrade,M.C.; Vaz,N.M.; Faria,A.M.C.
The gut mucosa is a major site of contact with antigens from food and microbiota. Usually, these daily contacts with natural antigens do not result in inflammatory reactions; instead they result in a state of systemic hyporesponsiveness named oral tolerance. Inflammatory bowel diseases (IBD) are associated with the breakdown of the immunoregulatory mechanisms that maintain oral tolerance. Several animal models of IBD/colitis are available. In mice, these include targeted disruptions of the genes encoding cytokines, T cell subsets or signaling proteins. Colitis can also be induced by intrarectal administration of chemical substances such as 2,4,6-trinitrobenzene sulfonic acid in 50% ethanol. We report here a novel model of colitis induced by intrarectal...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ethanol; Colitis; Oral tolerance; Cytokines.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900013

Therapeutic concentration of morphine reduces oxidative stress in glioma cell line
Provedor de dados: 56
Autores: Almeida,M.B.; Costa-Malaquias,A.; Nascimento,J.L.M.; Oliveira,K.R.; Herculano,A.M.; Crespo-López,M.E.
Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphine; Glia; Oxidative stress; Hydrogen peroxide; Lipid peroxidation; Opiod.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500398

The 42-kDa coat protein of Andean potato mottle virus acts as a transcriptional activator in yeast
Provedor de dados: 56
Autores: Vidal,M.S.; Margis,R.
Interactions of viral proteins play an important role in the virus life cycle, especially in capsid assembly. Andean potato mottle comovirus (APMoV) is a plant RNA virus with a virion formed by two coat proteins (CP42 and CP22). Both APMoV coat protein open reading frames were cloned into pGBT9 and pGAD10, two-hybrid system vectors. HF7c yeast cells transformed with the p9CP42 construct grew on yeast dropout selection media lacking tryptophan and histidine. Clones also exhibited ß-galactosidase activity in both qualitative and quantitative assays. These results suggest that CP42 protein contains an amino acid motif able to activate transcription of His3 and lacZ reporter genes in Saccharomyces cerevisiae. Several deletions of the CP42 gene were cloned into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Andean potato mottle virus; Virus assembly; Coat protein; Transactivation; Yeast two-hybrid system.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400001

Integrins in vascular development
Provedor de dados: 56
Autores: Hynes,R.O.; Bader,B.L.; Hodivala-Dilke,K.
Many growth factors and their protein kinase receptors play a role in regulating vascular development. In addition, cell adhesion molecules, such as integrins and their ligands in the extracellular matrix, play important roles in the adhesion, migration, proliferation, survival and differentiation of the cells that form the vasculature. Some integrins are known to be regulated by angiogenic growth factors and studies with inhibitors of integrin functions and using strains of mice lacking specific integrins clearly implicate some of these molecules in vasculogenesis and angiogenesis. However, the data are incomplete and sometimes discordant and it is unclear how angiogenic growth factors and integrin-mediated adhesive events cooperate in the diverse cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vasculogenesis; Angiogenesis; Integrins; Knockout mice.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500002

The role of calcium, calcium-activated K+ channels, and tyrosine/kinase in psoralen-evoked responses in human melanoma cells
Provedor de dados: 56
Autores: Isoldi,M.C.; Pereira,E.A.; Visconti,M.A.; Castrucci,A.M.L.
8-Methoxy psoralen (8-MOP) exerts a short-term (24 h) mitogenic action, and a long-term (48-72 h) anti-proliferative and melanogenic action on two human melanoma cell lines, SK-Mel 28 and C32TG. An increase of intracellular calcium concentration was observed by spectrofluorometry immediately after the addition of 0.1 mM 8-MOP to both cell lines, previously incubated with calcium probe fluo-3 AM (5 µM). The intracellular Ca2+ chelator BAPTA/AM (1 µM) blocked both early (mitogenic) and late (anti-proliferative and melanogenic) 8-MOP effects on both cell lines, thus revealing the importance of the calcium signal in both short- and long-term 8-MOP-evoked responses. Long-term biological assays with 5 and 10 mM tetraethylammonium chloride (TEA, an inhibitor of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 8-[Methoxy]-psoralen; SK-Mel 28 cell line; C32TG cell line; Cell proliferation; Cell signaling; Melanoma.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400013

Effect of the aerobic capacity on the validity of the anaerobic threshold for determination of the maximal lactate steady state in cycling
Provedor de dados: 56
Autores: Denadai,B.S.; Figueira,T.R.; Favaro,O.R.P.; Gonçalves,M.
The maximal lactate steady state (MLSS) is the highest blood lactate concentration that can be identified as maintaining a steady state during a prolonged submaximal constant workload. The objective of the present study was to analyze the influence of the aerobic capacity on the validity of anaerobic threshold (AT) to estimate the exercise intensity at MLSS (MLSS intensity) during cycling. Ten untrained males (UC) and 9 male endurance cyclists (EC) matched for age, weight and height performed one incremental maximal load test to determine AT and two to four 30-min constant submaximal load tests on a mechanically braked cycle ergometer to determine MLSS and MLSS intensity. AT was determined as the intensity corresponding to 3.5 mM blood lactate. MLSS...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Anaerobic threshold; Lactate steady state; Aerobic capacity; Cycling.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000015

Morphological changes of the jejunal mucosa in protracted diarrhea and their correlation with disease duration, weight loss and serum albumin levels
Provedor de dados: 56
Autores: Péret-Filho,L.A.; Brasileiro-Filho,G.; Penna,F.J.
The pathogenesis of protracted diarrhea is multifactorial. In developing countries, intestinal infectious processes seem to play an important role in triggering the syndrome. Thirty-four children aged 1 to 14 months, mean 6.5 months, with protracted diarrhea were studied clinically and in terms of small intestinal mucosal morphology. Mild, moderate or severe hypotrophy of the jejunal mucosa was detected in 82% of cases, and mucosal atrophy was observed in 12%. The intensity of the morphological changes of the jejunal mucosa correlated negatively with serum albumin levels. No correlation was detected between mucosal grading and duration of diarrhea or between mucosal grading and weight reported as percentile. After nutritional support was instituted, serial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protracted diarrhea; Biopsy; Jejunal mucosa; Serum albumin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900004

Epidermal growth factor receptor (EGFR) mutations in lung cancer: preclinical and clinical data
Provedor de dados: 56
Autores: Jorge,S.E.D.C.; Kobayashi,S.S.; Costa,D.B.
Lung cancer leads cancer-related mortality worldwide. Non-small-cell lung cancer (NSCLC), the most prevalent subtype of this recalcitrant cancer, is usually diagnosed at advanced stages, and available systemic therapies are mostly palliative. The probing of the NSCLC kinome has identified numerous nonoverlapping driver genomic events, including epidermal growth factor receptor (EGFR) gene mutations. This review provides a synopsis of preclinical and clinical data on EGFR mutated NSCLC and EGFR tyrosine kinase inhibitors (TKIs). Classic somatic EGFR kinase domain mutations (such as L858R and exon 19 deletions) make tumors addicted to their signaling cascades and generate a therapeutic window for the use of ATP-mimetic EGFR TKIs. The latter inhibit these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutation; Lung cancer; Non-small-cell lung cancer; EGFR; EGFR inhibitor; Precision therapies; Resistance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100929

Effect of microRNA-21 on the proliferation of human degenerated nucleus pulposus by targeting programmed cell death 4
Provedor de dados: 56
Autores: Chen,B.; Huang,S.G.; Ju,L.; Li,M.; Nie,F.F.; Zhang,Y.; Zhang,Y.H.; Chen,X.; Gao,F.
This study aims to explore the effect of microRNA-21 (miR-21) on the proliferation of human degenerated nucleus pulposus (NP) by targeting programmed cell death 4 (PDCD4) tumor suppressor. NP tissues were collected from 20 intervertebral disc degeneration (IDD) patients, and from 5 patients with traumatic spine fracture. MiR-21 expressions were tested. NP cells from IDD patients were collected and divided into blank control group, negative control group (transfected with miR-21 negative sequences), miR-21 inhibitor group (transfected with miR-21 inhibitors), miR-21 mimics group (transfected with miR-21 mimics) and PDCD4 siRNA group (transfected with PDCD4 siRNAs). Cell growth was estimated by Cell Counting Kit-8; PDCD4, MMP-2,MMP-9 mRNA expressions were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intervertebral disc degeneration; MicroRNA-21; Human nucleus pulposus cells; Matrix metalloproteinase-2; Matrix metalloproteinase-9; Target gene.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600602

Familial predisposition to hypertension and the association between urinary sodium excretion and blood pressure in a population-based sample of young adults
Provedor de dados: 56
Autores: Moraes,R.S.; Fuchs,F.D.; Dalla Costa,F.; Moreira,L.B.
The reasons for the inconsistent association between salt consumption and blood pressure levels observed in within-society surveys are not known. A total of 157 normotensive subjects aged 18 to 35 years, selected at random in a cross-sectional population-based survey, answered a structured questionnaire. They were classified as strongly predisposed to hypertension when two or more first-degree relatives had a diagnosis of hypertension. Anthropometric parameters were obtained and sitting blood pressure was determined with aneroid sphygmomanometers. Sodium and potassium excretion was measured by flame spectrophotometry in an overnight urine sample. A positive correlation between blood pressure and urinary sodium excretion was detected only in the group of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Familial predisposition to hypertension; Urinary sodium excretion; Salt sensitivity.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700010

Differentiation of autonomic reflex control begins with cellular mechanisms at the first synapse within the nucleus tractus solitarius
Provedor de dados: 56
Autores: Andresen,M.C.; Doyle,M.W.; Bailey,T.W.; Jin,Y.-H.
Visceral afferents send information via cranial nerves to the nucleus tractus solitarius (NTS). The NTS is the initial step of information processing that culminates in homeostatic reflex responses. Recent evidence suggests that strong afferent synaptic responses in the NTS are most often modulated by depression and this forms a basic principle of central integration of these autonomic pathways. The visceral afferent synapse is uncommonly powerful at the NTS with large unitary response amplitudes and depression rather than facilitation at moderate to high frequencies of activation. Substantial signal depression occurs through multiple mechanisms at this very first brainstem synapse onto second order NTS neurons. This review highlights new approaches to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sensory; Vanilloid; Glutamate; Presynaptic modulation; Autonomic; Visceral; Potassium currents.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400012

Addressing conflicts of interest in the research paper: a societal demand in contemporary science?
Provedor de dados: 56
Autores: Vasconcelos,S.M.R.; Cassimiro,M.C.; Martins,M.F.M.; Palácios,M.
In the last decade, dialogue between science and society has found a forum in an increasing number of publications on topics such as public engagement with science and public trust in science. Concerning the latter, issues that include cases of research misconduct, accountability in research, and conflicts of interest (COIs) have shaped global discussions on the communication of science. In the publication setting, the perception that hiding COIs and/or not managing them well may affect public trust in the research record has grown among editors. We conducted a search for editorials addressing COIs between 1989 and 2011, using four major databases: Medline/PubMed, Embase, Scopus, and Web of Knowledge. We explored the content of these editorials and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conflicts of interest; Contemporary science; Science policy; Science education; Research integrity; Science and society.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201007

Use of magnitude-squared coherence to identify the maximum driving response band of the somatosensory evoked potential
Provedor de dados: 56
Autores: Infantosi,A.F.C.; Melges,D.B.; Tierra-Criollo,C.J.
The present study proposes to apply magnitude-squared coherence (MSC) to the somatosensory evoked potential for identifying the maximum driving response band. EEG signals, leads [Fpz'-Cz'] and [C3'-C4'], were collected from two groups of normal volunteers, stimulated at the rate of 4.91 (G1: 26 volunteers) and 5.13 Hz (G2: 18 volunteers). About 1400 stimuli were applied to the right tibial nerve at the motor threshold level. After applying the anti-aliasing filter, the signals were digitized and then further low-pass filtered (200 Hz, 6th order Butterworth and zero-phase). Based on the rejection of the null hypothesis of response absence (MSC(f) &gt; 0.0060 with 500 epochs and the level of significance set at a = 0.05), the beta and gamma bands, 15-66 Hz,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma oscillations; Somatosensory response detection; Tibial nerve; Magnitude-squared coherence.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200011

The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction
Provedor de dados: 56
Autores: de-Souza,W.; de-Carvalho,T.U.; de-Melo,E.T.; Soares,C.P.; Coimbra,E.S.; Rosestolato,C.T.; Ferreira,S.R.; Vieira,M.
In this communication we review the results obtained with the confocal laser scanning microscope to characterize the interaction of epimastigote and trypomastigote forms of Trypanosoma cruzi and tachyzoites of Toxoplasma gondii with host cells. Early events of the interaction process were studied by the simultaneous localization of sites of protein phosphorylation, revealed by immunocytochemistry, and sites of actin assembly, revealed by the use of labeled phaloidin. The results obtained show that proteins localized in the interaction sites are phosphorylated. The process of formation of the parasitophorous vacuole was monitored by labeling the host cell surface with fluorescent probes for lipids (PKH26), proteins (DTAF) and sialic acid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Confocal laser scanning microscopy; Host cell-parasite interaction; Trypanosoma cruzi; Toxoplasma gondii.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100015

Insights on the epigenetic mechanisms underlying pulmonary arterial hypertension
Provedor de dados: 56
Autores: Luna,R.C.P.; de Oliveira,Y.; Lisboa,J.V.C.; Chaves,T.R.; de Araújo,T.A.M.; de Sousa,E.E.; Miranda Neto,M.; Pirola,L.; Braga,V.A.; de Brito Alves,J.L.
Pulmonary arterial hypertension (PAH), characterized by localized increased arterial blood pressure in the lungs, is a slow developing long-term disease that can be fatal. PAH is characterized by inflammation, vascular tone imbalance, pathological pulmonary vascular remodeling, and right-sided heart failure. Current treatments for PAH are palliative and development of new therapies is necessary. Recent and relevant studies have demonstrated that epigenetic processes may exert key influences on the pathogenesis of PAH and may be promising therapeutic targets in the prevention and/or cure of this condition. The aim of the present mini-review is to summarize the occurrence of epigenetic-based mechanisms in the context of PAH physiopathology, focusing on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epigenetic; Pulmonary arterial hypertension; DNA Methylation; Histone acetylation; MiRNAs.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200301

Snake venom metalloproteinases and disintegrins: interactions with cells
Provedor de dados: 56
Autores: Kamiguti,A.S.; Zuzel,M.; Theakston,R.D.G.
Metalloproteinases and disintegrins are important components of most viperid and crotalid venoms. Large metalloproteinases referred to as MDC enzymes are composed of an N-terminal Metalloproteinase domain, a Disintegrin-like domain and a Cys-rich C-terminus. In contrast, disintegrins are small non-enzymatic RGD-containing cysteine-rich polypeptides. However, the disintegrin region of MDC enzymes bears a high degree of structural homology to that of the disintegrins, although it lacks the RGD motif. Despite these differences, both components share the property of being able to recognize integrin cell surface receptors and thereby to inhibit integrin-dependent cell reactions. Recently, several membrane-bound MDC enzymes, closely related to soluble venom MDC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venom; Metalloproteinase; Disintegrin; Platelet; Integrin; Signaling.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700001

The use of light-emitting diodes to stimulate mitochondrial function and liver regeneration of partially hepatectomized rats
Provedor de dados: 56
Autores: Castro-e-Silva,T.; Castro-e-Silva,O.; Kurachi,C.; Ferreira,J.; Zucoloto,S.; Bagnato,V.S.
The biostimulating effect of laser radiation has been observed in many areas of Medicine. However, there are still several questions to be answered, among them the importance of light coherence in the stimulatory process. In the present study, we used light-emitting diodes (LED) to promote the stimulation of liver regeneration after partial hepatectomy in rats. Fourteen male Wistar rats weighing 200-250 g were submitted to partial hepatectomy (70%) followed by LED light irradiation (630 nm) of the remaining part of the liver at two doses, i.e., 10 (N = 7) and 140 (N = 7) J/cm². A group irradiated with laser, 590 nm (N = 7, 15 J/cm²) was performed for the study of proliferating cell nuclear antigen-labeling index. Data are reported as mean ± SEM....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver; Mitochondria; Laser; Light-emitting diode; Liver regeneration; Proliferating cell nuclear antigen.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800006

Identification of IgE and IgG1 specific antigens in Echinococcus granulosus cyst fluid
Provedor de dados: 56
Autores: Li,S.; Qian,R.; Wang,S.; Ye,J.; Zheng,H.
Cystic echinococcosis (CE) is an anthropozoonotic disease with worldwide distribution and is caused by the cestode Echinococcus granulosus. Anaphylactic shock induced by CE rupture is a serious complication especially in patients with hydatid infections, as the resulting leakage of fluid contains highly toxic endogenous antigen. We aimed to isolate and identify the antigens of specific IgE and IgG1 (sIgE and sIgG1) in E. granulosus cyst fluid (EgCF). Crude antigen for EgCF was prepared from E. granulosus-infected sheep liver. Antigens were separated and identified by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D SDS-PAGE), two-dimensional gel electrophoresis (2-DE), and immunoblotting. Results of 1D SDS-PAGE and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cystic echinococcosis; Anaphylactic shock; Specific antigens; Specific antibody.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700701

Hereditary motor and autonomic neuronopathy 1 maps to chromosome 20q13.2-13.3
Provedor de dados: 56
Autores: Marques Jr.,W.; Davis,M.B.; Abou-Sleiman,P.M.; Marques,V.D.; Silva Jr.,W.A.; Zago,M.A.; Sobreira,C.S.; Barreira,A.A.
The spinal muscular atrophies (SMA) or hereditary motor neuronopathies result from the continuous degeneration and death of spinal cord lower motor neurons, leading to progressive muscular weakness and atrophy. We describe a large Brazilian family exhibiting an extremely rare, late-onset, dominant, proximal, and progressive SMA accompanied by very unusual manifestations, such as an abnormal sweating pattern, and gastrointestinal and sexual dysfunctions, suggesting concomitant involvement of the autonomic nervous system. We propose a new disease category for this disorder, `hereditary motor and autonomic neuronopathy', and attribute the term, `survival of motor and autonomic neurons 1' (SMAN1) to the respective locus that was mapped to a 14.5 cM region on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spinal muscular atrophy; Hereditary motor neuronopathies; Autonomic neuropathies.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100022

Relationship between the prevalence of anti-glutamic acid decarboxylase autoantibodies and duration of type 1 diabetes mellitus in Brazilian patients
Provedor de dados: 56
Autores: Rodacki,M.; Zajdenverg,L.; Albernaz,M.S.; Bencke-Gonçalves,M.R.; Milech,A.; Oliveira,J.E.P.
The objective of the present study was to determine whether the duration of disease has any influence on the prevalence of glutamic acid decarboxylase autoantibodies (GADA) in Brazilian patients with type 1 diabetes (T1D) and variable disease duration. We evaluated 83 patients with T1D. All participants were interviewed and blood was obtained for GADA measurement by a commercial radioimmunoassay (RSR Limited, Cardiff, UK). Four groups of patients were established according to disease duration: A) 1-5 years of disease (N = 24), B) 6-10 years of disease (N = 19), C) 11-15 years of disease (N = 25), and D) &gt;15 years of disease (N = 15). GADA prevalence and its titers were determined in each group. GADA was positive in 38 patients (45.8%) and its frequency...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-GAD65 auto-antibodies; Type 1 diabetes mellitus; Time from diagnosis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100008

Antimicrobial resistance among invasive Haemophilus influenzae strains: results of a Brazilian study carried out from 1996 through 2000
Provedor de dados: 56
Autores: Casagrande,S.T.; Landgraf,I.M.; Kobata,A.M.M.; Zanella,R.C.; Bokermann,S.
A total of 1712 strains of Haemophilus influenzae isolated from patients with invasive diseases were obtained from ten Brazilian states from 1996 to 2000. ß-Lactamase production was assessed and the minimum inhibitory concentrations (MIC) of ampicillin, chloramphenicol, ceftriaxone and rifampin were determined using a method for broth microdilution of Haemophilus test medium. The prevalence of strains producing ß-lactamase ranged from 6.6 to 57.7%, with an overall prevalence of 18.4%. High frequency of ß-lactamase-mediated ampicillin resistance was observed in Distrito Federal (25%), São Paulo (21.7%) and Paraná (18.5%). Of the 1712 strains analyzed, none was ß-lactamase negative, ampicillin resistant. A total of 16.8% of the strains were resistant to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haemophilus influenzae; Meningitis; Antimicrobial resistance.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100006

Genome features of Leptospira interrogans serovar Copenhageni
Provedor de dados: 56
Autores: Nascimento,A.L.T.O.; Verjovski-Almeida,S.; Van Sluys,M.A.; Monteiro-Vitorello,C.B.; Camargo,L.E.A.; Digiampietri,L.A.; Harstkeerl,R.A.; Ho,P.L.; Marques,M.V.; Oliveira,M.C.; Setubal,J.C.; Haake,D.A.; Martins,E.A.L.
We report novel features of the genome sequence of Leptospira interrogans serovar Copenhageni, a highly invasive spirochete. Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in mammals. Genomic sequence analysis reveals the presence of a competent transport system with 13 families of genes encoding for major transporters including a three-member component efflux system compatible with the long-term survival of this organism. The leptospiral genome contains a broad array of genes encoding regulatory system, signal transduction and methyl-accepting chemotaxis proteins, reflecting the organism's ability to respond to diverse environmental stimuli. The identification of a complete set...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira interrogans; Outer membrane proteins; Lipopolysaccharides; Transport; Regulatory systems.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400003

Complementarity of radioautographic and immunohistochemical techniques for localizing neuroreceptors at the light and electron microscopy level
Provedor de dados: 56
Autores: Beaudet,A.
To assess relationships between neuropeptide-binding sites and receptor proteins in rat brain, the distribution of radioautographically labeled somatostatin and neurotensin-binding sites was compared to that of immunolabeled sst2A and NTRH receptor subtypes, respectively. By light microscopy, immunoreactive sst2A receptors were either confined to neuronal perikarya and dendrites or diffusely distributed in tissue. By electron microscopy, areas expressing somatodendritic sst2A receptors displayed only low proportions of membrane-associated, as compared to intracellular, receptors. Conversely, regions displaying diffuse sst2A labeling exhibited higher proportions of membrane-associated than intracellular receptors. Furthermore, the former showed only low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurotensin; Somatostatin; Internalization; Neuropeptides; Autoradiography; Ultrastructure.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200005

Resistance exercise improves hippocampus-dependent memory
Provedor de dados: 56
Autores: Cassilhas,R.C.; Lee,K.S.; Venâncio,D.P.; Oliveira,M.G.M.; Tufik,S.; Mello,M.T.
It has been demonstrated that resistance exercise improves cognitive functions in humans. Thus, an animal model that mimics this phenomenon can be an important tool for studying the underlying neurophysiological mechanisms. Here, we tested if an animal model for resistance exercise was able to improve the performance in a hippocampus-dependent memory task. In addition, we also evaluated the level of insulin-like growth factor 1/insulin growth factor receptor (IGF-1/IGF-1R), which plays pleiotropic roles in the nervous system. Adult male Wistar rats were divided into three groups (N = 10 for each group): control, SHAM, and resistance exercise (RES). The RES group was submitted to 8 weeks of progressive resistance exercise in a vertical ladder apparatus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physical exercise; Passive avoidance; Insulin-like growth factor 1; Resistance training; Hippocampus; Memory.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200015

Collagen content, but not the ratios of collagen type III/I mRNAs, differs among hypertensive, alcoholic, and idiopathic dilated cardiomyopathy
Provedor de dados: 56
Autores: Soufen,H.N.; Salemi,V.M.C.; Aneas,I.M.S.; Ramires,F.J.A.; Benício,A.M.D.; Benvenuti,L.A.; Krieger,J.E.; Mady,C.
Cardiac interstitial fibrosis may contribute to ventricular dysfunction and the prognosis of patients with dilated cardiomyopathy. The objective of the present study was to determine if total myocardial collagen content and collagen type III/I (III/I ratio) mRNAs differ in hypertensive, alcoholic, and idiopathic dilated cardiomyopathy subjects. Echocardiography and exercise cardiopulmonary testing were performed in patients with idiopathic (N = 22), hypertensive (N = 12), and alcoholic (N = 11) dilated cardiomyopathy. Morphometric analysis of collagen was performed in fragments obtained by endomyocardial biopsy with picrosirius red staining. The collagen III/I ratio was determined by reverse transcription polymerase chain reaction. Samples of controls (N =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcohol; Heart failure; Hypertension; Dilated cardiomyopathy; Collagen.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200009

Central chemoreceptors and neural mechanisms of cardiorespiratory control
Provedor de dados: 56
Autores: Takakura,A.C.; Damasceno,R.S.; Falquetto,B.; Totola,L.T.; Sobrinho,C.R.; Ragioto,D.T.; Zolezi,F.P.
The arterial partial pressure (P CO2) of carbon dioxide is virtually constant because of the close match between the metabolic production of this gas and its excretion via breathing. Blood gas homeostasis does not rely solely on changes in lung ventilation, but also to a considerable extent on circulatory adjustments that regulate the transport of CO2 from its sites of production to the lungs. The neural mechanisms that coordinate circulatory and ventilatory changes to achieve blood gas homeostasis are the subject of this review. Emphasis will be placed on the control of sympathetic outflow by central chemoreceptors. High levels of CO2 exert an excitatory effect on sympathetic outflow that is mediated by specialized chemoreceptors such as the neurons...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemoreflex; Cardiorespiratory responses; Ventrolateral medulla; Sympathetic nerve activity; Phrenic nerve activity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900009

Chromatic spatial contrast sensitivity estimated by visual evoked cortical potential and psychophysics
Provedor de dados: 56
Autores: Barboni,M.T.S.; Gomes,B.D.; Souza,G.S.; Rodrigues,A.R.; Ventura,D.F.; Silveira,L.C.L.
The purpose of the present study was to measure contrast sensitivity to equiluminant gratings using steady-state visual evoked cortical potential (ssVECP) and psychophysics. Six healthy volunteers were evaluated with ssVECPs and psychophysics. The visual stimuli were red-green or blue-yellow horizontal sinusoidal gratings, 5° × 5°, 34.3 cd/m2 mean luminance, presented at 6 Hz. Eight spatial frequencies from 0.2 to 8 cpd were used, each presented at 8 contrast levels. Contrast threshold was obtained by extrapolating second harmonic amplitude values to zero. Psychophysical contrast thresholds were measured using stimuli at 6 Hz and static presentation. Contrast sensitivity was calculated as the inverse function of the pooled cone contrast threshold. ssVECP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contrast sensitivity; Color vision; Steady-state visual evoked cortical potential; Visual thresholds; Spatial vision; Psychophysics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200154

Biphasic modulation of insulin receptor substrate-1 during goitrogenesis
Provedor de dados: 56
Autores: Grozovsky,R.; Morales,M.M.; Carvalho,D.P.
Insulin receptor substrate-1 (IRS-1) is the main intracellular substrate for both insulin and insulin-like growth factor I (IGF-I) receptors and is critical for cell mitogenesis. Thyrotropin is able to induce thyroid cell proliferation through the cyclic AMP intracellular cascade; however, the presence of either insulin or IGF-I is required for the mitogenic effect of thyroid-stimulating hormone (TSH) to occur. The aim of the present study was to determine whether thyroid IRS-1 content is modulated by TSH in vivo. Strikingly, hypothyroid goitrous rats, which have chronically high serum TSH levels (control, C = 2.31 ± 0.28; methimazole (MMI) 21d = 51.02 ± 6.02 ng/mL, N = 12 rats), when treated with 0.03% MMI in drinking water for 21 days, showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid; Goiter; Insulin receptor substrate; Insulin; Thyrotropin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500011

Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line
Provedor de dados: 56
Autores: Gimba,E.R.P.; Chemale,G.; Farias,S.S.; Zaha,A.
We report here for the first time the structure and function of a promoter from a cestode. The ability of DNA fragments respectively encompassing the 935-bp and 524-bp regions upstream from the ATG codon from the EgactI and EgactII actin genes of Echinococcus granulosus to promote transcription was studied in the NIH3T3 mouse cell line. The results of transfection assays showed that both regions have strong promoter activity in these cells. The fragments were tested in both orientations and the 524-bp fragment of EgactII presented a bidirectional promoter activity. Deletion analysis of EgactI and EgactII promoters indicated the presence of regulatory regions containing putative silencer elements. These results indicate that both EgactI and EgactII...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Echinococcus granulosus; Actin; Promoters; Cestode; Gene expression; Deletions.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100004

Immunopathology of the duodenal mucosa of HIV-positive patients during combined antiretroviral therapy
Provedor de dados: 56
Autores: Machado,F.R.; Pagliari,C.; Caiafa,H.; Tapajós,R.; Duarte,M.I.S.
The objective of the present study was to evaluate the duodenal mucosa of HIV-infected patients during antiretroviral therapy. This was an observational study conducted on HIV-positive patients and a control group. Group 1 comprised 22 HIV-negative individuals while 38 HIV-positive individuals were classified according to the CDC 1993 classification into group 2 (A1 or A2) or group 3 (B2, A3, B3, C2, C3). All subjects were submitted to upper gastrointestinal endoscopy with duodenal biopsies. Qualitative, semi-quantitative and quantitative histological analyses were performed. Results were considered significant when P &lt; 0.05. A higher prevalence of inflammatory infiltrate and eosinophilia was observed in the HIV group, together with a reduction in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal histopathology; Cytokines; Human immunodeficiency virus; Acquired immunodeficiency syndrome; Antiretroviral therapy; CD4+.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100013

Heart rate recovery after exercise: relations to heart rate variability and complexity
Provedor de dados: 56
Autores: Javorka,M.; Zila,I.; Balhárek,T.; Javorka,K.
Physical exercise is associated with parasympathetic withdrawal and increased sympathetic activity resulting in heart rate increase. The rate of post-exercise cardiodeceleration is used as an index of cardiac vagal reactivation. Analysis of heart rate variability (HRV) and complexity can provide useful information about autonomic control of the cardiovascular system. The aim of the present study was to ascertain the association between heart rate decrease after exercise and HRV parameters. Heart rate was monitored in 17 healthy male subjects (mean age: 20 years) during the pre-exercise phase (25 min supine, 5 min standing), during exercise (8 min of the step test with an ascending frequency corresponding to 70% of individual maximal power output) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise recovery; Heart rate variability; Entropy; Spectral analysis; Parasympathetic system.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800018

Maturation of pig oocytes in vitro in a medium with pyruvate
Provedor de dados: 56
Autores: Gonzales-Figueroa,H.; Gonzales-Molfino,H.M.
The aim of in vitro maturation oocyte systems is to produce oocytes of comparable quality to those derived in vivo. The present study was designed to examine the surface morphological changes of the cumulus-oocyte complex (COC) and nuclear maturation in a culture system containing pyruvate. Ovaries were obtained from a slaughterhouseand transported to the laboratory within 2 h at 35-39ºC,and rinsed three times in 0.9% NaCl. The COCs were harvested from the ovaries and in vitro maturation was evaluated in San Marcos (SM) medium, a chemically defined culture system containing 22.3 mM sodium pyruvate. Oocytes were cultured in SM, SM + porcine follicular fluid (pFF) and in SM + pFF + gonadotropins (eCG and hCG) for 20-22 h and then without hormonal supplements...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pig oocytes; In vitro maturation; Pyruvate; Cumulus cells.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600008

Roles of monocyte chemotactic protein 1 and nuclear factor-κB in immune response to spinal tuberculosis in a New Zealand white rabbit model
Provedor de dados: 56
Autores: Guo,X.H.; Bai,Z.; Qiang,B.; Bu,F.H.; Zhao,N.
This study aimed to explore the roles of monocyte chemotactic protein 1 (MCP-1) and nuclear factor kappa B (NF-κB) in immune response to spinal tuberculosis in a New Zealand white rabbit model. Forty-eight New Zealand white rabbits were collected and divided into four groups: experimental group (n=30, spinal tuberculosis model was established), the sham group (n=15, sham operation was performed) and the blank group (n=3). The qRT-PCR assay and western blotting were applied to detect the mRNA and protein expressions of MCP-1 and NF-κB in peripheral blood. ELISA was used to measure serum levels of MCP-1, NF-κB, IFN-γ, IL-2, IL-4, and IL-10. Flow cytometry was adopted to assess the distributions of CD4+, CD8+ lymphocytes and CD4+ CD25+ Foxp3 lymphocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monocyte chemotactic protein 1; Nuclear factor-κB; Spinal tuberculosis; Immune response.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300602

Influence of cueing on the preparation and execution of untrained and trained complex motor responses
Provedor de dados: 56
Autores: Alouche,S.R.; Sant’Anna,G.N.; Biagioni,G.; Ribeiro-do-Valle,L.E.
This study investigated the influence of cueing on the performance of untrained and trained complex motor responses. Healthy adults responded to a visual target by performing four sequential movements (complex response) or a single movement (simple response) of their middle finger. A visual cue preceded the target by an interval of 300, 1000, or 2000 ms. In Experiment 1, the complex and simple responses were not previously trained. During the testing session, the complex response pattern varied on a trial-by-trial basis following the indication provided by the visual cue. In Experiment 2, the complex response and the simple response were extensively trained beforehand. During the testing session, the trained complex response pattern was performed in all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cueing; Cue-target interval; Response complexity; Response practice; Reaction time; Movement time.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500008

Identification of fungemia agents using the polymerase chain reaction and restriction fragment length polymorphism analysis
Provedor de dados: 56
Autores: Santos,M.S.; Souza,E.S.; Junior,R.M.S.; Talhari,S.; Souza,J.V.B.
Prompt and specific identification of fungemia agents is important in order to define clinical treatment. However, in most cases conventional culture identification can be considered to be time-consuming and not without errors. The aim of the present study was to identify the following fungemia agents: Candida albicans, Candida parapsilosis, Candida tropicalis, Candida glabrata, Cryptococcus neoformans, Cryptococcus gattii, and Histoplasma capsulatum using the polymerase chain reaction and restriction fragment length polymorphism analysis (PCR/RFLP). More specifically: a) to evaluate 3 different amplification regions, b) to investigate 3 different restriction enzymes, and c) to use the best PCR/RFLP procedure to indentify 60 fungemia agents from a culture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR/RFLP; Fungemia agents; Identification; Ddel.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800003

Inotropic effects of extracts of Psidium guajava L. (guava) leaves on the guinea pig atrium
Provedor de dados: 56
Autores: Conde Garcia,E.A.; Nascimento,V.T.; Santiago Santos,A.B.
Many pharmacological effects have been ascribed to extracts of Psidium guajava L. (guava) leaves. However, in spite of its widespread use in Brazilian folk medicine and a reasonable number of scientific reports about it, we could not find any study dealing with its action on the mammalian myocardium. In the present study, by measuring isometric force, we observed that the crude extract of P. guajava (water-alcohol extract obtained by macerating dry leaves) depresses the guinea pig atrial contractility in a concentration-dependent fashion (N = 8 hearts, 15 trials). The compound with cardiac activity was concentrated by extraction in a Soxhlet apparatus using 17 M glacial acetic acid after removing the less polar fractions (hexane, chloroform, acetone,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart contractility; Guinea pig; Myocardial inotropism; Psidium guajava L.; Folk medicine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500014

The mental health of graduate students at the Federal University of São Paulo: a preliminary report
Provedor de dados: 56
Autores: Nogueira-Martins,L.A.; Fagnani Neto,R.; Macedo,P.C.M.; Cítero,V.A.; Mari,J.J.
We present data regarding the care provided to graduate level health professionals at the mental health center of the Federal University of São Paulo. From September 1996 to September 2003, 146 graduate students (99 in the Master's degree program and 47 in the Doctoral program) were attended. This population was predominantly female (68.5%), with a mean (± SD) age of 28.6 ± 4.42 years, not married (71.9%). Most of the subjects were professionals who had not graduated from the Federal University (78.1%). The students who sought help for psychological and/or psychiatric problems were classified into two categories: situational-adaptive crises and psychopathological crises. The main diagnoses were depression and anxiety disorders (44%) causing 4.5% of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mental health; Graduate education; Burnout; Suicide; Occupational stress; Mental health program.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000011

Structural basis for the pathophysiology of lipoprotein(a) in the athero-thrombotic process
Provedor de dados: 56
Autores: Anglés-Cano,E.
Lipoprotein Lp(a) is a major and independent genetic risk factor for atherosclerosis and cardiovascular disease. The essential difference between Lp(a) and low density lipoproteins (LDL) is apolipoprotein apo(a), a glycoprotein structurally similar to plasminogen, the precursor of plasmin, the fibrinolytic enzyme. This structural homology endows Lp(a) with the capacity to bind to fibrin and to membrane proteins of endothelial cells and monocytes, and thereby to inhibit plasminogen binding and plasmin generation. The inhibition of plasmin generation and the accumulation of Lp(a) on the surface of fibrin and cell membranes favor fibrin and cholesterol deposition at sites of vascular injury. Moreover, insufficient activation of TGF-ß due to low plasmin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Apolipoprotein(a); Thrombosis; Atheroma; Plasminogen; Lipoprotein; Fibrin; Fibrinolysis.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100002

Retraction notice for: “Ginsenoside Rd inhibits IL-1β-induced inflammation and degradation of intervertebral disc chondrocytes by increasing IL1RAP ubiquitination” [Braz J Med Biol Res (2019) 52(9): e8525]
Provedor de dados: 56
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200951

Antinociceptive effects of the essential oil of Croton zehntneri in mice
Provedor de dados: 56
Autores: Oliveira,A.C.; Leal-Cardoso,J.H.; Santos,C.F.; Morais,S.M.; Coelho-de-Souza,A.N.
Croton zehntneri is an aromatic plant native to Northeastern Brazil, where it is often used in folk medicine. In the present study the antinociceptive effects of the essential oil of Croton zehntneri (EOCz) were evaluated in mice. EOCz administered orally at doses of 100 and 300 mg/kg reduced paw licking time in the second phase of the formalin test from the control value of 41.61 ± 8.62 to 12.01 ± 7.97 and 6.57 ± 3.42 s, respectively. During the first phase of the formalin test only 300 mg/kg induced a significant alteration (from 58.2 ± 7.02, control, to 28.7 ± 4.73 s). The number of contortions in response to intraperitoneal injections of acetic acid did not differ significantly between controls (80.6 ± 9.01) and experimental (300 mg/kg body weight)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Croton zehntneri; Essential oil; Analgesic; Antinociception; Formalin test; Hot-plate test.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100016

Nitro-fatty acids: novel anti-inflammatory lipid mediators
Provedor de dados: 56
Autores: Rubbo,H.
Nitro-fatty acids are formed and detected in human plasma, cell membranes, and tissue, modulating metabolic as well as inflammatory signaling pathways. Here we discuss the mechanisms of nitro-fatty acid formation as well as their key chemical and biochemical properties. The electrophilic properties of nitro-fatty acids to activate anti-inflammatory signaling pathways are discussed in detail. A critical issue is the influence of nitroarachidonic acid on prostaglandin endoperoxide H synthases, redirecting arachidonic acid metabolism and signaling. We also analyze in vivo data supporting nitro-fatty acids as promising pharmacological tools to prevent inflammatory diseases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitro-fatty acids; Antioxidants; Nitric oxide; Inflammation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900728

Involvement of hippocampal AMPA glutamate receptor changes and the cAMP/protein kinase A/CREB-P signalling pathway in memory consolidation of an avoidance task in rats
Provedor de dados: 56
Autores: Bernabeu,R.; Cammarota,M.; Izquierdo,I.; Medina,J.H.
Training in step-down inhibitory avoidance (0.3-mA footshock) is followed by biochemical changes in rat hippocampus that strongly suggest an involvement of quantitative changes in glutamate AMPA receptors, followed by changes in the dopamine D1 receptor/cAMP/protein kinase A (PKA)/CREB-P signalling pathway in memory consolidation. AMPA binding to its receptor and levels of the AMPA receptor-specific subunit GluR1 increase in the hippocampus within the first 3 h after training (20-70%). Binding of the specific D1 receptor ligand, SCH23390, and cAMP levels increase within 3 or 6 h after training (30-100%). PKA activity and CREB-P levels show two peaks: a 35-40% increase 0 h after training, and a second increase 3-6 h later (35-60%). The results correlate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutamate AMPA receptors; CAMP/PKA/CREB pathway; Memory formation; Rat hippocampus.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800008

The distribution of presumptive thoracic paraganglionic tissue in the common marmoset (Callithrix jacchus)
Provedor de dados: 56
Autores: Clarke,J.A.; Daly,M. de B.
The aortic-pulmonary regions (APR) of seven adult marmosets (Callithrix jacchus) and the region of the right subclavian artery of a further three marmosets were diffusion-fixed with 10% buffered formol-saline solution. In both regions serial 5-µm sections were cut and stained by the Martius yellow, brilliant crystal scarlet and soluble blue method. Presumptive thoracic paraganglionic (PTP) tissue was only observed in the APR. PTP tissue was composed of small groups of cells that varied in size and number. The distribution of the groups of cells was extremely variable, so much so that it would be misleading to attempt to classify their position; they were not circumscribed by a connective tissue capsule, but were always related to the thoracic branches of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Presumptive paraganglionic cells; Thorax; Marmoset.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400005

A tobacco cDNA reveals two different transcription patterns in vegetative and reproductive organs
Provedor de dados: 56
Autores: Silva,I. da; Angelo,P.C.S.; Molfetta,J.B.; Ferraz,M.T.; Silva,L.L.P. da; Goldman,G.H.; Goldman,M.H.S.
In order to identify genes expressed in the pistil that may have a role in the reproduction process, we have established an expressed sequence tags project to randomly sequence clones from a Nicotiana tabacum stigma/style cDNA library. A cDNA clone (MTL-8) showing high sequence similarity to genes encoding glycine-rich RNA-binding proteins was chosen for further characterization. Based on the extensive identity of MTL-8 to the RGP-1a sequence of N. sylvestris, a primer was defined to extend the 5' sequence of MTL-8 by RT-PCR from stigma/style RNAs. The amplification product was sequenced and it was confirmed that MTL-8 corresponds to an mRNA encoding a glycine-rich RNA-binding protein. Two transcripts of different sizes and expression patterns were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine-rich protein; Nicotiana tabacum; Pistil expression; RNA-binding protein; Stress conditions; Virus infection.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800001

Heterogeneity of glomerular perfusion and filtration induced by epinephrine and norepinephrine
Provedor de dados: 56
Autores: Boim,M.A.; Stella,S.R.; Pereira,A.B.; Schor,N.
The role of catecholamines in the distribution of intrarenal blood flow and in single-nephron glomerular filtration rate (SNGFR) was evaluated in anesthetized Wistar rats by the Hanssen technique. Epinephrine (EPI) and norepinephrine (NOR) were infused to produce elevations of 20-30 mmHg in mean arterial pressure. Superficial and juxtamedullary nephron perfusion and filtration were determined by the presence of Prussian blue dye. In the control group, 100% of the nephrons presented a homogeneous pattern of perfusion and filtration. In contrast, a heterogeneous distribution of the dye was found even in the larger arteries (arciform and radial), indicating variable perfusion and filtration in both superficial and juxtamedullary nephrons. The effects of EPI...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catecholamines; Intrarenal blood flow; Glomerular function; Hanssen technique; Micropuncture.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800017

High frequencies of plexiform neurofibromas, mental retardation, learning difficulties, and scoliosis in Brazilian patients with neurofibromatosis type 1
Provedor de dados: 56
Autores: Trovó-Marqui,A.B.; Goloni-Bertollo,E.M.; Valério,N.I.; Pavarino-Bertelli,E.C.; Muniz,M.P.; Teixeira,M.F.; Antonio,J.R.; Tajara,E.H.
A clinical study of Brazilian patients with neurofibromatosis type 1 (NF1) was performed in a multidisciplinary Neurofibromatosis Program called CEPAN (Center of Research and Service in Neurofibromatosis). Among 55 patients (60% females, 40% males) who met the NIH criteria for the diagnosis of NF1, 98% had more than six café-au-lait patches, 94.5% had axillary freckling, 45% had inguinal freckling, and 87.5% had Lisch nodules. Cutaneous neurofibromas were observed in 96%, and 40% presented plexiform neurofibromas. A positive family history of NF1 was found in 60%, and mental retardation occurred in 35%. Some degree of scoliosis was noted in 49%, 51% had macrocephaly, 40% had short stature, 76% had learning difficulties, and 2% had optic gliomas....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurofibromatosis type I; Plexiform neurofibroma; Mental retardation; Learning difficulties; Scoliosis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900020

Occupational therapy in spinocerebellar ataxia type 3: an open-label trial
Provedor de dados: 56
Autores: Silva,R.C.R.; Saute,J.A.M.; Silva,A.C.F.; Coutinho,A.C.O.; Saraiva-Pereira,M.L.; Jardim,L.B.
Occupational therapy (OT) is a profession concerned with promoting health and well-being through occupation, by enabling handicapped people to participate in the activities of everyday life. OT is part of the clinical rehabilitation of progressive genetic neurodegenerative diseases such as spinocerebellar ataxias; however, its effects have never been determined in these diseases. Our aim was to investigate the effect of OT on both physical disabilities and depressive symptoms of spinocerebellar ataxia type 3 (SCA3) patients. Genomically diagnosed SCA3 patients older than 18 years were invited to participate in the study. Disability, as evaluated by functional independence measurement and Barthel incapacitation score, Hamilton Rating Scale for Depression,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spinocerebellar ataxia 3; Occupational therapy; Rehabilitation; Depression; Machado-Joseph disease; Polyglutamine diseases.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600003

Variations in gastric emptying of liquid elicited by acute blood volume changes in awake rats
Provedor de dados: 56
Autores: Gondim,F. de-A.A.; Oliveira,G.R. de-; Graça,J.R.V.; Cavalcante,D.I.M.; Souza,M.A.N.; Santos,A.A.; Rola,F.H.
We have observed that acute blood volume expansion increases the gastroduodenal resistance to the flow of liquid in anesthetized dogs, while retraction decreases it (Santos et al. (1991) Acta Physiologica Scandinavica, 143: 261-269). This study evaluates the effect of blood volume expansion and retraction on the gastric emptying of liquid in awake rats using a modification of the technique of Scarpignato (1980) (Archives Internationales de Pharmacodynamie et de Therapie, 246: 286-294). Male Wistar rats (180-200 g) were fasted for 16 h with water ad libitum and 1.5 ml of the test meal (0.5 mg/ml phenol red solution in 5% glucose) was delivered to the stomach immediately after random submission to one of the following protocols: 1) normovolemic control (N =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood volume; Hypervolemia; Hemorrhage; Central venous pressure; Gastric emptying; Gastrointestinal motility.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700013

Duration-controlled swimming exercise training induces cardiac hypertrophy in mice
Provedor de dados: 56
Autores: Evangelista,F.S.; Brum,P.C.; Krieger,J.E.
Exercise training associated with robust conditioning can be useful for the study of molecular mechanisms underlying exercise-induced cardiac hypertrophy. A swimming apparatus is described to control training regimens in terms of duration, load, and frequency of exercise. Mice were submitted to 60- vs 90-min session/day, once vs twice a day, with 2 or 4% of the weight of the mouse or no workload attached to the tail, for 4 vs 6 weeks of exercise training. Blood pressure was unchanged in all groups while resting heart rate decreased in the trained groups (8-18%). Skeletal muscle citrate synthase activity, measured spectrophotometrically, increased (45-58%) only as a result of duration and frequency-controlled exercise training, indicating that endurance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise training; Swimming; Cardiac hypertrophy; Mice; Myocardium.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200018

Frequency of 8 CFTR gene mutations in cystic fibrosis patients in Minas Gerais, Brazil, diagnosed by neonatal screening
Provedor de dados: 56
Autores: Perone,C.; Medeiros,G.S.; del Castillo,D.M.; de Aguiar,M.J.B.; Januário,J.N.
The nature and frequency of cystic fibrosis mutations in Brazil is not uniform due to the highly varied ethnic composition of the population. The average frequency of the F508del mutation has been reported to be 48.6%. Other common mutations in Brazil are G542X, R1162X, and N1303K. The aim of this study was to analyze the frequency of 8 mutations (F508del, G542X, R1162X, N1303K, W1282X, G85E, 3120+1G&gt;A, and 711+1G&gt;T) in a sample of 111 newborn patients with cystic fibrosis diagnosed by the Cystic Fibrosis Neonatal Screening Program of Minas Gerais State. The mutations were tested by allele-specific oligonucleotide PCR with specially designed primers. An allele frequency of 48.2% was observed for the F508del mutation, and allele frequencies of 5.41,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CFTR gene; Cystic fibrosis; Mutations; Neonatal screening; F508del.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200003

Multigenerational Brazilian family with malignant hyperthermia and a novel mutation in the RYR1 gene
Provedor de dados: 56
Autores: Matos,A.R.; Sambuughin,N.; Rumjanek,F.D.; Amoedo,N.D.; Cunha,L.B.P.; Zapata-Sudo,G.; Sudo,R.T.
Malignant hyperthermia (MH) is a pharmacogenetic disease triggered in susceptible individuals by the administration of volatile halogenated anesthetics and/or succinylcholine, leading to the development of a hypermetabolic crisis, which is caused by abnormal release of Ca2+ from the sarcoplasmic reticulum, through the Ca2+ release channel ryanodine receptor 1 (RyR1). Mutations in the RYR1 gene are associated with MH in the majority of susceptible families. Genetic screening of a 5-generation Brazilian family with a history of MH-related deaths and a previous MH diagnosis by the caffeine halothane contracture test (CHCT) in some individuals was performed using restriction and sequencing analysis. A novel missense mutation, Gly4935Ser, was found in an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malignant hyperthermia; Mutation; Ryanodine receptor; Calcium channel.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200016

Late-life depression, heart failure and frontal white matter hyperintensity: a structural magnetic resonance imaging study
Provedor de dados: 56
Autores: Almeida,J.R.C.; Alves,T.C.T.F.; Wajngarten,M.; Rays,J.; Castro,C.C.; Cordeiro,Q.; Telles,R.M.S.; Fraguas,R.J.; Busatto,G.F.
The relevance of the relationship between cardiac disease and depressive symptoms is well established. White matter hyperintensity, a bright signal area in the brain on T2-weighted magnetic resonance imaging scans, has been separately associated with cardiovascular risk factors, cardiac disease and late-life depression. However, no study has directly investigated the association between heart failure, major depressive symptoms and the presence of hyperintensities. Using a visual assessment scale, we have investigated the frequency and severity of white matter hyperintensities identified by magnetic resonance imaging in eight patients with late-life depression and heart failure, ten patients with heart failure without depression, and fourteen healthy...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Major depressive disorder; Old age; Magnetic resonance imaging; Cardiac failure; Cerebrovascular disease; Cardiovascular risk factors.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300014

Role of Leishmania (Leishmania) amazonensis amastigote glycosphingolipids in macrophage infectivity
Provedor de dados: 56
Autores: Tanaka,A.K.; Gorin,P.A.J.; Takahashi,H.K.; Straus,A.H.
The role of glycosphingolipids (GSLs) present in amastigote forms of Leishmania (Leishmania) amazonensis during infection of macrophages was analyzed, with particular emphasis on GSLs presenting the terminal Galpß1-3Galpa disaccharide. Macrophage invasion by L. (L.) amazonensis amastigotes was reduced by 37% when the disaccharide Galpß1-3Galp (1 mM) was added to the culture medium. The putative macrophage receptor/lectin for ß-Gal-globotriaosylceramide (Galpß1-3Galpa1-4Galpß1-4Glc pß1-1Cer) and other structurally related GSLs from L. (L.) amazonensis amastigotes were analyzed by micelles and parasite binding assay to peritoneal macrophage proteins fractionated by SDS-PAGE under nonreducing conditions. Micelles containing purified amastigote GSLs or a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania (Leishmania) amazonensis; Amastigote; Glycosphingolipid; Macrophage binding protein.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600008

Behavioral meaningful opioidergic stimulation activates kappa receptor gene expression
Provedor de dados: 56
Autores: Teodorov,E.; Ferrari,M.F.R.; Fior-Chadi,D.R.; Camarini,R.; Felício,L.F.
The periaqueductal gray (PAG) has been reported to be a location for opioid regulation of pain and a potential site for behavioral selection in females. Opioid-mediated behavioral and physiological responses differ according to the activity of opioid receptor subtypes. The present study investigated the effects of the peripheral injection of the kappa-opioid receptor agonist U69593 into the dorsal subcutaneous region of animals on maternal behavior and on Oprk1 gene activity in the PAG of female rats. Female Wistar rats weighing 200-250 g at the beginning of the study were randomly divided into 2 groups for maternal behavior and gene expression experiments. On day 5, pups were removed at 7:00 am and placed in another home cage that was distant from their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Real-time reverse-transcription polymerase chain reaction; Opioid receptor; Gene expression; Opioid sensitivity; Kappa opioid receptor.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000015

Effects of metformin treatment on luteal phase progesterone concentration in polycystic ovary syndrome
Provedor de dados: 56
Autores: Meenakumari,K.J.; Agarwal,S.; Krishna,A.; Pandey,L.K.
The causes of luteal phase progesterone deficiency in polycystic ovary syndrome (PCOS) are not known. To determine the possible involvement of hyperinsulinemia in luteal phase progesterone deficiency in women with PCOS, we examined the relationship between progesterone, luteinizing hormone (LH) and insulin during the luteal phase and studied the effect of metformin on luteal progesterone levels in PCOS. Patients with PCOS (19 women aged 18-35 years) were treated with metformin (500 mg three times daily) for 4 weeks prior to the test cycle and throughout the study period, and submitted to ovulation induction with clomiphene citrate. Blood samples were collected from control (N = 5, same age range as PCOS women) and PCOS women during the late follicular (one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polycystic ovary syndrome; Metformin; Luteal phase defect; Progesterone.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100007

Effect of intraoperative lung-protective mechanical ventilation on pulmonary oxygenation function and postoperative pulmonary complications after laparoscopic radical gastrectomy
Provedor de dados: 56
Autores: Liu,Jing; Meng,Zhipeng; lv,Ran; Zhang,Yaping; Wang,Gaojian; Xie,Junran.
This study aimed to observe the effects of lung-protective ventilation (LPV) on oxygenation index (OI) and postoperative pulmonary complications (PPCs) after laparoscopic radical gastrectomy in middle-aged and elderly patients. A total of 120 patients who were scheduled to undergo laparoscopic radical gastrectomy with an expected time of &gt;3 h were randomly divided into conventional ventilation (CV group) with tidal volume (TV) of 10 mL/kg without positive end-expiratory pressure (PEEP), and lung-protective ventilation (PV group) with 7 mL/kg TV and personal level of PEEP with regular recruitment maneuver every 30 min. Measurements of OI, modified clinical pulmonary infection score (mCPIS), and PPCs were assessed during the perioperative period....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung-protective ventilation strategy; Laparoscopic radical gastrectomy; Middle-aged and elderly people; Lung oxygenation function; Postoperative pulmonary complication.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600611

Two novel Mg(II)-based and Zn(II)-based complexes: inhibiting growth of human liver cancer cells
Provedor de dados: 56
Autores: Liu,N.; Ding,D.; Wang,L.; Zhao,H.; Zhu,L.; Geng,X.
Two new Mg(II)-based and Zn(II)-based coordination polymers, {[Mg3(BTB)(DMA)4](DMA)2}n (1, H3BTB=1,3,5-benzenetrisbenzoic acid, DMA=N,N-dimethylacetamide) and {(H2NMe2)2[Zn3(BTB)2(OH)(Im)](DMF)9(MeOH)7}n (2, Im=imidazole, DMF=N,N-dimethylformamide), have been successfully synthesized and structurally characterized under solvothermal conditions. 1 contains a linear [Mg3(COO)6] cluster that connected by the fully deprotonated BTB3- ligands to give a kgd-type 2D bilayer structure; 2 represents a microporous 3D pillar-layered system based on the binuclear Zn units and pillared Im ligands, which shows a (3,5)-connected hms topological net. In addition, in vitro anticancer activities of compounds 1 and 2 on 4 human liver cancer cells (HB611, HHCC, BEL-7405 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coordination polymer; Pillar-layered; Liver cancer.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200614

Therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia using Fe2O3 nanoparticles
Provedor de dados: 56
Autores: Yan,S.Y.; Chen,M.M.; Fan,J.G.; Wang,Y.Q.; Du,Y.Q.; Hu,Y.; Xu,L.M.
This study aimed to investigate the therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia (MFH) using Fe2O3 nanoparticles. Hepatocarcinoma SMMC-7721 cells cultured in vitro were treated with ferrofluid containing Fe2O3 nanoparticles and irradiated with an alternating radio frequency magnetic field. The influence of the treatment on the cells was examined by inverted microscopy, MTT and flow cytometry. To study the therapeutic mechanism of the Fe2O3 MFH, Hsp70, Bax, Bcl-2 and p53 were detected by immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR). It was shown that Fe2O3 MFH could cause cellular necrosis, induce cellular apoptosis, and significantly inhibit cellular growth, all of which...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fe2O3; Magnetic nanoparticles; Magnetic fluid hyperthermia; Hepatocarcinoma cells; Liver cancer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100947

Treadmill exercise testing of asymptomatic men and women without evidence of heart disease
Provedor de dados: 56
Autores: Chalela,W.A.; Fukushima,R.B.; Araujo,F.; Lima,A.C.P.; Moffa,P.J.; Mansur,A.J.
The aim of this study was to test the hypothesis of differences in performance including differences in ST-T wave changes between healthy men and women submitted to an exercise stress test. Two hundred (45.4%) men and 241 (54.6%) women (mean age: 38.7 ± 11.0 years) were submitted to an exercise stress test. Physiologic and electrocardiographic variables were compared by the Student t-test and the chi-square test. To test the hypothesis of differences in ST-segment changes, data were ranked with functional models based on weighted least squares. To evaluate the influence of gender and age on the diagnosis of ST-segment abnormality, a logistic model was adjusted; P &lt; 0.05 was considered to be significant. Rate-pressure product, duration of exercise and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ST-T wave changes; Exercise stress test; Asymptomatic; Women.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200018

Enhanced anti-tumor effect of a gene gun-delivered DNA vaccine encoding the human papillomavirus type 16 oncoproteins genetically fused to the herpes simplex virus glycoprotein D
Provedor de dados: 56
Autores: Diniz,M.O.; Ferreira,L.C.S.
Anti-cancer DNA vaccines have attracted growing interest as a simple and non-invasive method for both the treatment and prevention of tumors induced by human papillomaviruses. Nonetheless, the low immunogenicity of parenterally administered vaccines, particularly regarding the activation of cytotoxic CD8+ T cell responses, suggests that further improvements in both vaccine composition and administration routes are still required. In the present study, we report the immune responses and anti-tumor effects of a DNA vaccine (pgD-E7E6E5) expressing three proteins (E7, E6, and E5) of the human papillomavirus type 16 genetically fused to the glycoprotein D of the human herpes simplex virus type 1, which was administered to mice by the intradermal (id) route...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gene gun; DNA vaccine; HPV-16; Anti-cancer vaccine.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500007

The challenge of vector development in gene therapy
Provedor de dados: 56
Autores: Dani,S.U.
Gene therapy is the treatment of diseases based on the transfer of genetic information. Agents that carry or deliver DNA to target cells are called vectors (Latin vector: carrier, deliverer). Ideally, a vector should accommodate an unlimited amount of inserted DNA, lack the ability of autonomous replication of its own DNA, be easily manufactured, and be available in concentrated form. Secondly, it should have the ability to target specific cell types or to limit its gene expression to specific cell types, and to achieve sustained gene expression in the long term or in a controlled fashion. Finally, it should not be toxic or immunogenic. Such a vector does not exist and none of the DNA delivery systems so far available for in vivo gene transfer is perfect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene therapy; Gene transfer; DNA delivery; Viral vectors; Diposomes; Vector development.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200001

N-acetylcysteine downregulates phosphorylated p-38 expression but does not reverse the increased superoxide anion levels in the spinal cord of rats with neuropathic pain
Provedor de dados: 56
Autores: Horst,A.; de Souza,J.A.; Santos,M.C.Q.; Riffel,A.P.K.; Kolberg,C.; Ribeiro,M.F.M.; de Fraga,L.S.; Partata,W.A.
We determined the effect of N-acetylcysteine (NAC) on the expression of the phosphorylated p38 (p-p38) protein and superoxide anion generation (SAG), two important players in the processing of neuropathic pain, in the lumbosacral spinal cord of rats with chronic constriction injury (CCI)-induced neuropathic pain. The sciatic functional index (SFI) was also measured to assess the functional recovery post-nerve lesion. Thirty-six male Wistar rats were divided equally into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right common sciatic nerve), which received 2, 4, or 8...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sciatic functional index; Chronic constriction; Sciatic nerve; Mitogen-activated protein kinase; Antinociception; Mechanical threshold.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200605

Protein-mediated surface structuring in biomembranes
Provedor de dados: 56
Autores: Maggio,B.; Rosetti,C.M.; Borioli,G.A.; Fanani,M.L.; Del Boca,M.
The lipids and proteins of biomembranes exhibit highly dissimilar conformations, geometrical shapes, amphipathicity, and thermodynamic properties which constrain their two-dimensional molecular packing, electrostatics, and interaction preferences. This causes inevitable development of large local tensions that frequently relax into phase or compositional immiscibility along lateral and transverse planes of the membrane. On the other hand, these effects constitute the very codes that mediate molecular and structural changes determining and controlling the possibilities for enzymatic activity, apposition and recombination in biomembranes. The presence of proteins constitutes a major perturbing factor for the membrane sculpturing both in terms of its surface...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipid monolayers; Sphingomyelinase; C-Fos; Segregated lipid domains; Lipid-protein interaction; Anphitropic proteins.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200002

Gene expression profiling of clinical stages II and III breast cancer
Provedor de dados: 56
Autores: Folgueira,M.A.A.K.; Brentani,H.; Katayama,M.L.H.; Patrão,D.F.C.; Carraro,D.M.; Mourão Netto,M.; Barbosa,E.M.; Caldeira,J.R.F.; Abreu,A.P.S.; Lyra,E.C.; Kaiano,J.H.L.; Mota,L.D.; Campos,A.H.J.F.M.; Maciel,M.S.; Dellamano,M.; Caballero,O.L.S.D.; Brentani,M.M.
Clinical stage (CS) is an established indicator of breast cancer outcome. In the present study, a cDNA microarray platform containing 692 genes was used to identify molecular differences between CSII and CSIII disease. Tumor samples were collected from patients with CSII or CSIII breast cancer, and normal breast tissue was collected from women without invasive cancer. Seventy-eight genes were deregulated in CSIII tumors and 22 in CSII tumors when compared to normal tissue, and 20 of them were differentially expressed in both CSII and CSIII tumors. In addition, 58 genes were specifically altered in CSIII and expression of 6 of them was tested by real time RT-PCR in another cohort of patients with CSII or CSIII breast cancer and in women without cancer....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; CDNA microarray; Clinical stage; Gene expression.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800013

Urinary secretory IgA after nutritional rehabilitation
Provedor de dados: 56
Autores: Teodósio,M.R.; Oliveira,E.C.M.
We studied the secretory IgA (sIgA) response of the mucosal urinary tract of malnourished children before and after nutritional rehabilitation. sIgA concentration (mg/l) was determined by ELISA in 187 children aged 3 months to 5 years. The children, who frequented a day care center, were divided into four groups, according to nutritional status: 57 were eutrophic, 49 were undergrown, 57 were moderately malnourished and 24 were severely malnourished. In addition, dip slide (Urotube, Roche) and dip-stick (Combur 9-Boehringer) tests showed that children had no bacteriuria or any other urinary abnormalities. Plasma albumin concentration (g/dl) was significantly lower (P&amp;lt;0.005) in the severely malnourished group (mean 3.0 ± 0.3 SD) than in the eutrophic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urinary tract; Local immune response; Secretory IgA; Nutritional status.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400007

Gender and age differences in polysomnography findings and sleep complaints of patients referred to a sleep laboratory
Provedor de dados: 56
Autores: Silva,A.; Andersen,M.L.; De Mello,M.T.; Bittencourt,L.R.A.; Peruzzo,D.; Tufik,S.
Our objective was to examine the effet of gender on the sleep pattern of patients referred to a sleep laboratory. The data (questionnaires and polysomnographic recordings) were collected from a total of 2365 patients (1550 men and 815 women). The polysomnography permits an objective assessment of the sleep pattern. We included only polysomnography exams obtained with no more than one recording system in order to permit normalization of the data. Men had a significantly higher body mass index than women (28.5 ± 4.8 vs 27.7 ± 6.35 kg/m²) and had a significantly higher score on the Epworth Sleepiness Scale (10.8 ± 5.3 vs 9.5 ± 6.0), suggesting daytime sleepiness. Women had a significantly higher sleep latency than men, as well as a higher rapid eye movement...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep; Gender; Polysomnography; Apnea; Insomnia; Database.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200005

Association of hepatic nuclear factor-4 in the apolipoprotein B promoter: a preliminary report
Provedor de dados: 56
Autores: Nóvak,E.M.; Dantas,K.C.; Charbel,C.E.; Bydlowski,S.P.
Previous studies have examined the arrangement of regulatory elements along the apolipoprotein B (apoB) promoter region (-3067 to +940) and a promoter fragment extending from nucleotides -150 to +124 has been demonstrated to be essential for transcriptional activation of the apoB gene in hepatic and intestinal cells. It has also been shown that transcriptional activation of apoB requires a synergistic interaction between hepatic nuclear factor-4 (HNF-4) and CCAAT/enhancer-binding protein a (C/EBPa) transcription factors. Here, we have examined the hypothesis that HNF-4 factor binding to DNA may induce a DNA helix bend, thus facilitating the communication with a C/EBPa factor located one helix turn from this HNF-4 factor in the apoB promoter. A gel...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Apolipoprotein B promoter; HNF-4; C/EBPa.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100006

Rapid eye movement (REM) sleep deprivation reduces rat frontal cortex acetylcholinesterase (EC 3.1.1.7) activity
Provedor de dados: 56
Rapid eye movement (REM) sleep deprivation induces several behavioral changes. Among these, a decrease in yawning behavior produced by low doses of cholinergic agonists is observed which indicates a change in brain cholinergic neurotransmission after REM sleep deprivation. Acetylcholinesterase (Achase) controls acetylcholine (Ach) availability in the synaptic cleft. Therefore, altered Achase activity may lead to a change in Ach availability at the receptor level which, in turn, may result in modification of cholinergic neurotransmission. To determine if REM sleep deprivation would change the activity of Achase, male Wistar rats, 3 months old, weighing 250-300 g, were deprived of REM sleep for 96 h by the flower-pot technique (N = 12). Two additional...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: REM sleep deprivation; Frontal cortex; Acetylcholinesterase.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500012

Bone marrow fibroblasts in patients with advanced lung cancer
Provedor de dados: 56
Autores: Chasseing,N.A.; Hofer,E.; Bordenave,R.H.; Shanley,C.; Rumi,L.S.
In a previous study we demonstrated that the incidence of fibroblast colony-forming units (CFU-F) was very low in bone marrow primary cultures from the majority of untreated advanced non-small lung cancer patients (LCP) compared to normal controls (NC). For this reason, we studied the ability of bone marrow stromal cells to achieve confluence in primary cultures and their proliferative capacity following four continuous subcultures in consecutive untreated LCP and NC. We also evaluated the production of interleukin-1ß (IL-1ß) and prostaglandin E2 (PGE2) by pure fibroblasts. Bone marrow was obtained from 20 LCP and 20 NC. A CFU-F assay was used to investigate the proliferative and confluence capacity. Levels of IL-1ß and PGE2 in conditioned medium (CM) of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibroblasts; Interleukin-1; Prostaglandin E2; Cancer.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100014

Analysis of the diagnostic presentation profile, parathyroidectomy indication and bone mineral density follow-up of Brazilian patients with primary hyperparathyroidism
Provedor de dados: 56
Autores: Oliveira,U.E.M.; Ohe,M.N.; Santos,R.O.; Cervantes,O.; Abrahão,M.; Lazaretti-Castro,M.; Vieira,J.G.H.; Hauache,O.M.
Primary hyperparathyroidism is an endocrine disorder with variable clinical expression, frequently presenting as asymptomatic hypercalcemia in Western countries but still predominantly as a symptomatic disease in developing countries. The objective of this retrospective study was to describe the diagnostic presentation profile, parathyroidectomy indication and post-surgical bone mineral density follow-up of patients with primary hyperparathyroidism seen at a university hospital. We found 115 patients (92 women, median age 56 years) with primary hyperparathyroidism diagnosed during the last 20 years. We defined symptomatic patients based on the presence of any classical symptom affecting bone, kidney or the neuromuscular system. Surgical criteria followed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Primary hyperparathyroidism; Hypercalcemia; Clinical presentation; Parathyroidectomy; Bone mineral density.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400010

Angiotensin-(1-7) potentiates the coronary vasodilatatory effect of bradykinin in the isolated rat heart
Provedor de dados: 56
Autores: Almeida,A.P.; Frábregas,B.C.; Madureira,M.M.; Santos,R.J.S.; Campagnole-Santos,M.J.; Santos,R.A.S.
It has been shown that angiotensin-(1-7) (Ang-(1-7)) infusion potentiates the bradykinin (BK)-induced hypotensive response in conscious rats. The present study was conducted to identify Ang-(1-7)-BK interactions in the isolated rat heart perfused according to the Langendorff technique. Hearts were excised and perfused through the aortic stump under a constant flow with Krebs-Ringer solution and the changes in perfusion pressure and heart contractile force were recorded. Bolus injections of BK (2.5, 5, 10 and 20 ng) produced a dose-dependent hypotensive effect. Ang-(1-7) added to the perfusion solution (2 ng/ml) did not change the perfusion pressure or the contractile force but doubled the hypotensive effect of the lower doses of BK. The BK-potentiating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin-(1-7); Bradykinin; Coronary artery; Rat heart; L-NAME; Prostaglandins; Bradykinin potentiation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600012

Long-term efficacy of a rural community-based integrated intervention for prevention and management of chronic obstructive pulmonary disease: a cluster randomized controlled trial in China's rural areas
Provedor de dados: 56
Autores: Yuan,X.; Tao,Y.; Zhao,J.P.; Liu,X.S.; Xiong,W.N.; Xie,J.G.; Ni,W.; Xu,Y.J.; Liu,H.G.
This study aimed to assess the efficacy of a rural community-based integrated intervention for early prevention and management of chronic obstructive pulmonary disease (COPD) in China. This 18-year cluster-randomized controlled trial encompassing 15 villages included 1008 patients (454 men and 40 women in the intervention group [mean age, 54 ± 10 years]; 482 men and 32 women in the control group [mean age, 53 ± 10 years]) with confirmed COPD or at risk for COPD. Villages were randomly assigned to the intervention or the control group, and study participants residing within the villages received treatment accordingly. Intervention group patients took part in a program that included systematic health education, smoking cessation counseling, and education on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic obstructive pulmonary disease; Randomized controlled trial; Integrated intervention; Rural area; Lung function; Smoking cessation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101023

Protective effects of organoselenium compounds against methylmercury-induced oxidative stress in mouse brain mitochondrial-enriched fractions
Provedor de dados: 56
Autores: Meinerz,D.F.; de Paula,M.T.; Comparsi,B.; Silva,M.U.; Schmitz,A.E.; Braga,H.C.; Taube,P.S.; Braga,A.L.; Rocha,J.B.T.; Dafre,A.L.; Farina,M.; Franco,J.L.; Posser,T.
We evaluated the potential neuroprotective effect of 1-100 µM of four organoselenium compounds: diphenyl diselenide, 3’3-ditri-fluoromethyldiphenyl diselenide, p-methoxy-diphenyl diselenide, and p-chloro-diphenyl diselenide, against methylmercury-induced mitochondrial dysfunction and oxidative stress in mitochondrial-enriched fractions from adult Swiss mouse brain. Methylmercury (10-100 µM) significantly decreased mitochondrial activity, assessed by MTT reduction assay, in a dose-dependent manner, which occurred in parallel with increased glutathione oxidation, hydroperoxide formation (xylenol orange assay) and lipid peroxidation end-products (thiobarbituric acid reactive substances, TBARS). The co-incubation with diphenyl diselenide (100 µM) completely...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylmercury; Mitochondria; Oxidative stress; Organoselenium compounds; Diphenyl diselenide.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100012

Perspectives of digestive pest control with proteinase inhibitors that mainly affect the trypsin-like activity of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae)
Provedor de dados: 56
Autores: Pereira,M.E.; Dörr,F.A.; Peixoto,N.C.; Lima-Garcia,J.F.; Dörr,F.; Brito,G.G.
The present study describes the main characteristics of the proteolytic activities of the velvetbean caterpillar, Anticarsia gemmatalis Hübner, and their sensitivity to proteinase inhibitors and activators. Midguts of last instar larvae reared on an artificial diet were homogenized in 0.15 M NaCl and centrifuged at 14,000 g for 10 min at 4ºC and the supernatants were used in enzymatic assays at 30ºC, pH 10.0. Basal total proteolytic activity (azocasein hydrolysis) was 1.14 ± 0.15 absorbance variation min-1 mg protein-1, at 420 nm; basal trypsin-like activity (N-benzoyl-L-arginine-p-nitroanilide, BApNA, hydrolysis) was 0.217 ± 0.02 mmol p-nitroaniline min-1 mg protein-1. The maximum proteolytic activities were observed at pH 10.5 using azocasein and at pH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticarsia gemmatalis (Lepidoptera: Noctuidae); Azocasein hydrolysis; BApNA hydrolysis; Proteinase inhibitors; Protein digestion; Serine-proteinases.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100010

Characterization of the interdependency between residues that bind the substrate in a β-glycosidase
Provedor de dados: 56
Autores: Tomassi,M.H.; Rozenfeld,J.H.K.; Gonçalves,L.M.; Marana,S.R.
The manner by which effects of simultaneous mutations combine to change enzymatic activity is not easily predictable because these effects are not always additive in a linear manner. Hence, the characterization of the effects of simultaneous mutations of amino acid residues that bind the substrate can make a significant contribution to the understanding of the substrate specificity of enzymes. In the β-glycosidase from Spodoptera frugiperda (Sfβgly), both residues Q39 and E451 interact with the substrate and this is essential for defining substrate specificity. Double mutants of Sfβgly (A451E39, S451E39 and S451N39) were prepared by site-directed mutagenesis, expressed in bacteria and purified using affinity chromatography. These enzymes were characterized...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Β-glycosidase; Substrate specificity; Site-directed mutagenesis; Spodoptera frugiperda.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100002

Autonomic processing of the cardiovascular reflexes in the nucleus tractus solitarii
Provedor de dados: 56
The nucleus tractus solitarii (NTS) receives afferent projections from the arterial baroreceptors, carotid chemoreceptors and cardiopulmonary receptors and as a function of this information produces autonomic adjustments in order to maintain arterial blood pressure within a narrow range of variation. The activation of each of these cardiovascular afferents produces a specific autonomic response by the excitation of neuronal projections from the NTS to the ventrolateral areas of the medulla (nucleus ambiguus, caudal and rostral ventrolateral medulla). The neurotransmitters at the NTS level as well as the excitatory amino acid (EAA) receptors involved in the processing of the autonomic responses in the NTS, although extensively studied, remain to be...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: L-glutamate; Excitatory amino acid receptors; NMDA receptors; Baroreflex; Chemoreflex; Bezold-Jarisch reflex; AP-5; Kynureic acid.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400015

Electroretinography in dogs using a fiber electrode prototype
Provedor de dados: 56
Autores: Pereira,A.L.; Montiani-Ferreira,F.; Santos,V.R.; Salomão,S.R.; Souza,C.; Berezovsky,A.
We compared two electroretinography (ERG) electrodes in dogs using ERG standards of the International Society for Clinical Electrophysiology of Vision (ISCEV). Ten healthy Yorkshire terrier dogs (mean age, 2.80 ± 1.42 years; 6 females) weighing 5.20 ± 1.56 kg were evaluated using an ERG system for veterinary use. Dark- and light-adapted ERG responses were recorded using an ERG-Jet electrode and a fiber electrode prototype. The examinations were performed during 2 visits, 3 weeks apart. Both electrodes (ERG-Jet or fiber prototype) were used on each animal and the first eye to be recorded (OD × OS) was selected randomly. Three weeks later the examination was repeated on the same animal switching the type of electrode to be used that day and the first eye to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electroretinography; Electrode; Retina; Canine; Prototype.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300257

Effect of different durations of overdistention on rat bladder function and morphology
Provedor de dados: 56
Autores: Jin,Xiao-Dong; Cao,Min; Zhou,Xie-Lai; Yin,Hong-Ping; Chen,Zhao-Dian; Xu,Ning; Jiang,Hai.
We investigated the contribution of the duration of overdistention (DOD) to rat bladder function and morphology and explored its possible molecular mechanisms. Bladder overdistention was induced in male Sprague-Dawley rats (200-250 g) by an infusion of saline. Forty rats were divided into 5 groups submitted to different DOD, i.e., 1, 2, 4, and 8 h, and control. Bladder function was evaluated by cystometry. Morphological changes were observed by light and transmission electron microscopy. Compared to control (44.567 ± 3.472 cmH2O), the maximum detrusor pressure of groups with 2-, 4- and 8-h DOD decreased significantly (means ± SEM): 32.774 ± 3.726, 31.321 ± 2.847, and 29.238 ± 3.724 cmH2O. With the increase of DOD, inflammatory infiltration and impairment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute urinary retention; Bladder overdistention; Urodynamics; Ultrastructure; Apoptosis; Rat.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000006

Cytotoxic effect of essential oil of thyme (Thymus broussonettii) on the IGR-OV1 tumor cells resistant to chemotherapy
Provedor de dados: 56
Autores: Ait M'Barek,L.; Ait Mouse,H.; Jaâfari,A.; Aboufatima,R.; Benharref,A.; Kamal,M; Bénard,J.; El Abbadi,N.; Bensalah,M.; Gamouh,A.; Chait,A.; Dalal,A.; Zyad,A.
The anti-tumor effect of the Moroccan endemic thyme (Thymus broussonettii) essential oil (EOT) was investigated in vitro using the human ovarian adenocarcinoma IGR-OV1 parental cell line OV1/P and its chemoresistant counterparts OV1/adriamycin (OV1/ADR), OV1/vincristine (OV1/VCR), and OV1/cisplatin (OV1/CDDP). All of these cell lines elicited various degrees of sensitivity to the cytotoxic effect of EOT. The IC50 values (mean ± SEM, v/v) were 0.40 ± 0.02, 0.39 ± 0.02, 0.94 ± 0.05, and 0.65 ± 0.03% for OV1/P, OV1/ADR, OV1/VCR, and OV1/CDDP, respectively. Using the DBA-2/P815 (H2d) mouse model, tumors were developed by subcutaneous grafting of tumor fragments of similar size obtained from P815 (murin mastocytoma cell line) injected in donor mouse....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essential oil; Thymus broussonettii; Ovarian cancer; Cytotoxicity; OV1/adriamycin; OV1/vincristine; OV1/cisplatin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100014

Telomere length analysis in monoclonal B-cell lymphocytosis and chronic lymphocytic leukemia Binet A
Provedor de dados: 56
Autores: Furtado,F.M.; Scheucher,P.S.; Santana,B.A.; Scatena,N.F.; Calado,R.T.; Rego,E.M.; Matos,D.M.; Falcão,R.P.
Monoclonal B-cell lymphocytosis (MBL) is an asymptomatic clinical entity characterized by the proliferation of monoclonal B cells not meeting the diagnosis criteria for chronic lymphocytic leukemia (CLL). MBL may precede the development of CLL, but the molecular mechanisms responsible for disease progression and evolution are not completely known. Telomeres are usually short in CLL and their attrition may contribute to disease evolution. Here, we determined the telomere lengths of CD5+CD19+ cells in MBL, CLL, and healthy volunteers. Twenty-one CLL patients, 11 subjects with high-count MBL, and 6 with low-count MBL were enrolled. Two hundred and sixty-one healthy volunteers aged 0 to 88 years were studied as controls. After diagnosis confirmation, a flow...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Chronic lymphocytic leukemia; Monoclonal B-cell lymphocytosis; Telomere length.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500603

Central injection of captopril inhibits the blood pressure response to intracerebroventricular choline
Provedor de dados: 56
Autores: Isbil-Buyukcoskun,N.; Gulec,G.; Ozluk,K.; Ulus,I.H.
In the present study, we investigated the involvement of the brain renin-angiotensin system in the effects of central cholinergic stimulation on blood pressure in conscious, freely moving normotensive rats. In the first step, we determined the effects of intracerebroventricular (icv) choline (50, 100 and 150 µg) on blood pressure. Choline increased blood pressure in a dose-dependent manner. In order to investigate the effects of brain renin-angiotensin system blockade on blood pressure increase induced by choline (150 µg, icv), an angiotensin-converting enzyme inhibitor, captopril (25 and 50 µg, icv), was administered 3 min before choline. Twenty-five µg captopril did not block the pressor effect of choline, while 50 µg captopril blocked it significantly....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Choline; Captopril; Intracerebroventricular choline; Blood pressure.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600018

Sleep patterns and sleep-related complaints of Brazilian interstate bus drivers
Provedor de dados: 56
Autores: Mello,M.T.; Santana,M.G.; Souza,L.M.; Oliveira,P.C.S.; Ventura,M.L.; Stampi,C.; Tufik,S.
Sleep-related complaints have become a highlight for physicians as well as public health administrators. Studies of sleep patterns and sleep-related complaints of shift workers have been useful in minimizing reduction in the quality of life due to the warping of the sleep-wake cycle. The objective of the present study was to assess patterns of sleep, sleep-related complaints as well as physical activity and scoring rates for depression and anxiety in interstate bus drivers. Data were obtained with a sleep questionnaire, with the Beck inventory for depression, and the State-Trait Anxiety Inventory (STAI). A total of 400 interstate bus drivers from the northern, southern, central-western and south-eastern regions of Brazil were interviewed. Sixty percent of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep; Sleep-related disorders; Shift workers; Depression; Anxiety; Physical activity.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100010

The miR-17-92 cluster regulates FOG-2 expression and inhibits proliferation of mouse embryonic cardiomyocytes
Provedor de dados: 56
Autores: Xiang,Rui; Lei,Han; Chen,Mianzhi; Li,Qinwei; Sun,Huan; Ai,Jianzhong; Chen,Tielin; Wang,Honglian; Fang,Yin; Zhou,Qin.
MicroRNAs (miRNAs) have gradually been recognized as regulators of embryonic development; however, relatively few miRNAs have been identified that regulate cardiac development. A series of recent papers have established an essential role for the miRNA-17-92 (miR-17-92) cluster of miRNAs in the development of the heart. Previous research has shown that the Friend of Gata-2 (FOG-2) is critical for cardiac development. To investigate the possibility that the miR-17-92 cluster regulates FOG-2 expression and inhibits proliferation in mouse embryonic cardiomyocytes we initially used bioinformatics to analyze 3’ untranslated regions (3’UTR) of FOG-2 to predict the potential of miR-17-92 to target it. We used luciferase assays to demonstrate that miR-17-5p and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNAs; MiR-17-92; FOG-2; Proliferation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200007

Anti-inflammatory and antispasmodic activity of Ipomoea imperati (Vahl) Griseb (Convolvulaceae)
Provedor de dados: 56
Autores: Paula,A.C.B.; Hayashi,L.S.S.; Freitas,J.C.
Ipomoea imperati (Convolvulaceae) lives on the sandy shores of the Brazilian coast and in other areas of the world. The anti-inflammatory activity of a methanol-water extract of the leaves of I. imperati was investigated in experimental models of acute and subchronic inflammation. Topical application of the extract (10 mg/ear) inhibited mouse ear edema induced by croton oil (89.0 ± 1.3% by the lipid fraction with an IC50 of 3.97 mg/ear and 57.0 ± 1.3% by the aqueous fraction with an IC50 of 3.5 mg/ear) and arachidonic acid (42.0 ± 2.0% with an IC50 of 4.98 mg/ear and 31.0 ± 2.0% with an IC50 of 4.72 mg/ear). Phospholipase A2, purified from Apis mellifera bee venom, was also inhibited by the extract (5.0 mg/ml lipid and aqueous fraction) in vitro in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-inflammatory activity; Antispasmodic activity; Arachidonic acid; Croton oil; Medicinal plants; Phospholipase A2.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100014

Cystatin C and renal function in pediatric kidney transplant recipients
Provedor de dados: 56
Autores: Franco,M.C.P.; Nagasako,S.S.; Machado,P.G.; Nogueira,P.C.K.; Pestana,J.O.M.; Sesso,R.
In clinical practice, the glomerular filtration rate (GFR) is often determined with serum creatinine. However, studies have shown cystatin C to be a better parameter for the diagnosis of impaired renal function. We compared GFR estimated by plasma cystatin C with GFR estimated by serum creatinine in a sample of 50 pediatric renal transplant recipients and 24 healthy children. The correlation between GFR estimated by serum creatinine and by cystatin C was significant (r = 0.75; P &lt; 0.001, Person’s correlation); however, in pediatric kidney transplant recipients, the GFR was 6.7 mL/min lower when determined using cystatin C rather than serum creatinine. Moreover, using GFR estimated by cystatin C we found that 42% of the pediatric kidney transplant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Children; Kidney transplant; Cystatin C; Creatinine; Glomerular filtration rate.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200017

Body fat and cholecalciferol supplementation in elderly homebound individuals
Provedor de dados: 56
Autores: Canto-Costa,M.H.S.; Kunii,I.; Hauache,O.M.
Vitamin D deficiency, observed mainly in the geriatric population, is responsible for loss of bone mass and increased risk of bone fractures. Currently, recommended doses of cholecalciferol are advised, but since there are few studies evaluating the factors that influence the serum levels of 25-hydroxyvitamin D (25(OH)D) following supplementation, we analyzed the relationship between the increase in serum 25(OH)D after supplementation and body fat. We studied a group of 42 homebound elderly subjects over 65 years old (31 women) in order to assess whether there is a need for adjustment of the doses of cholecalciferol administered to this group according to their adipose mass. Baseline measurements of 25(OH)D, intact parathyroid hormone and bone remodeling...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholecalciferol; Vitamin D insufficiency; Secondary hyperparathyroidism; Obesity; Body fat content.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100011

Thyroid hormone regulates protein expression in C6 glioma cells
Provedor de dados: 56
Autores: Trentin,A.G.; Alvarez-Silva,M.
Thyroid hormone (T3) is essential to normal brain development. Previously, we have shown that T3 induces cerebellar astrocyte proliferation. This effect is accompanied by alteration in glial fibrillary acidic protein (GFAP) and fibronectin organization. In the present study, we report that the C6 glioma cell line, which expresses GFAP and is classified as an undifferentiated astrocytic cell type, is a target for T3 action. The C6 monolayers were treated with 50 nM T3 for 3 days, after which the cells were maintained for 2 days without medium changes. In C6 cells, T3 induced the expression of proteins of 107, 73 and 62 kDa. The hormone also up-regulated protein bands of 100 (+50%), 37 (+50%) and 25.5 kDa (+50%) and down-regulated proteins of 94 (-100%),...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Thyroid hormone; Glioma; Protein synthesis; GFAP; Vimentin; Differentiation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000008

Animal model for age- and sex-related genotoxicity of diethylstilbestrol
Provedor de dados: 56
Autores: Fučić,A.; Stojkovic,R.; Katić,J.; Markovic,D.; Ferencic,Z.; Koršić,M.; Jazbec,A.M.; Gamulin,M.
Environmental xenoestrogens pose a significant health risk for all living organisms. There is growing evidence concerning the different susceptibility to xenoestrogens of developing and adult organisms, but little is known about their genotoxicity in pre-pubertal mammals. In the present study, we developed an animal model to test the sex- and age-specific genotoxicity of the synthetic estrogen diethylstilbestrol (DES) on the reticulocytes of 3-week-old pre-pubertal and 12-week-old adult BALB/CJ mice using the in vivo micronucleus (MN) assay. DES was administered intraperitoneally at doses of 0.05, 0.5, and 5 µg/kg for 3 days and animals were sampled 48, 72 and 96 h, and 2 weeks after exposure. Five animals were analyzed for each dose, sex, and age group....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diethylstilbestrol; Estrogen; Puberty; In vivo micronucleus assay; Sex; Genotoxicity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100015

Cyclic AMP increases the survival of ganglion cells in mixed retinal cell cultures in the absence of exogenous neurotrophic molecules, an effect that involves cholinergic activity
Provedor de dados: 56
Autores: Santos,R.C.C.; Araujo,E.G.
Natural cell death is a well-known degenerative phenomenon occurring during development of the nervous system. The role of trophic molecules produced by target and afferent cells as well as by glial cells has been extensively demonstrated. Literature data demonstrate that cAMP can modulate the survival of neuronal cells. Cultures of mixed retinal cells were treated with forskolin (an activator of the enzyme adenylyl cyclase) for 48 h. The results show that 50 µM forskolin induced a two-fold increase in the survival of retinal ganglion cells (RGCs) in the absence of exogenous trophic factors. This effect was dose dependent and abolished by 1 µM H89 (an inhibitor of protein kinase A), 1.25 µM chelerythrine chloride (an inhibitor of protein kinase C), 50 µM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ganglion cell survival; CAMP; Amacrine cholinergic cells; M1 receptor activity; Glial cells.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200011

Chlorophyll treatment combined with photostimulation increases glycolysis and decreases oxidative stress in the liver of type 1 diabetic rats
Provedor de dados: 56
Autores: Wunderlich,A.L.M.; Azevedo,S.C.S.F.; Yamada,L.A.; Bataglini,C.; Previate,C.; Campanholi,K.S.S.; Pereira,P.C.S.; Caetano,W.; Kaplum,V.; Nakamura,C.V.; Nakanishi,A.B.S.; Comar,J.F.; Pedrosa,M.M.D.; Godoi,V.A.F.
Photodynamic therapy (PDT) promotes cell death, and it has been successfully employed as a treatment resource for neuropathic complications of diabetes mellitus (T1DM) and hepatocellular carcinoma. The liver is the major organ involved in the regulation of energy homeostasis, and in pathological conditions such as T1DM, changes in liver metabolic pathways result in hyperglycemia, which is associated with multiple organic dysfunctions. In this context, it has been suggested that chlorophyll-a and its derivatives have anti-diabetic actions, such as reducing hyperglycemia, hyperinsulinemia, and hypertriglyceridemia, but these effects have not yet been proven. Thus, the biological action of PDT with chlorophyll-a on hepatic parameters related to energy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photopigment; Diabetes; Metabolism; Free Radicals; Phototherapy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100601

Amniotic fluid amino acid levels in non-immune hydrops fetalis: a case-control study
Provedor de dados: 56
Autores: Erdemoğlu,M.; Kuyumcuoglu,U.; Guzel,A.I.; Celik,Y.; Kale,E.
In a prospective case-control study, we compared the amniotic fluid amino acid levels in non-immune hydrops fetalis (NIHF) and normal fetuses. Eighty fetuses underwent amniocentesis for different reasons at the prenatal diagnosis unit of the Department of Obstetrics and Gynecology, Faculty of Medicine, Dicle University. Forty of these fetuses were diagnosed with NIHF. The study included 40 women each in the NIHF (mean age: 27.69 ± 4.56 years) and control (27.52 ± 5.49 years) groups, who had abnormal double- or triple-screening test values with normal fetuses with gestational ages of 23.26 ± 1.98 and 23.68 ± 1.49 weeks at the time of sample collection, respectively. Amniotic fluid amino acid concentrations (intra-assay variation: 2.26-7.85%; interassay...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-immune hydrops fetalis; Amniotic fluid; Amino acid levels.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700018

Volume and energy folding landscape of prion protein revealed by pressure
Provedor de dados: 56
Autores: Cordeiro,Y.; Kraineva,J.; Winter,R.; Silva,J.L.
The main hypothesis for prion diseases proposes that the cellular protein (PrP C) can be altered into a misfolded, ß-sheet-rich isoform, the PrP Sc (from scrapie). The formation of this abnormal isoform then triggers the transmissible spongiform encephalopathies. Here, we discuss the use of high pressure as a tool to investigate this structural transition and to populate possible intermediates in the folding/unfolding pathway of the prion protein. The latest findings on the application of high pressure to the cellular prion protein and to the scrapie PrP forms will be summarized in this review, which focuses on the energetic and volumetric properties of prion folding and conversion.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prion; High pressure; Structural conversion; Aggregation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800006

Production and characterization of a monoclonal antibody against an Ascaris suum allergenic component
Provedor de dados: 56
Autores: Pires,R.R.; Oshiro,T.M.; Itami,D.M.; Fernandes,I.; Macedo-Soares,M.F.
Ascaris suum allergenic components (PIII) separated by gel filtration chromatography of an adult worm extract were used to immunize BALB/c mice. Popliteal lymph node cells taken from the immunized animals were fused with SP2/O myeloma cells using polyethylene glycol (MW 1450) as fusogen. The hybridomas were cultured in HAT-containing medium and cloned at limiting dilutions. Supernatants from the growing hybrids were screened by ELISA using plates coated with PIII or the A. suum crude extract. The monoclonal antibody obtained, named MAC-3 (mouse anti-A. suum allergenic component), is an IgG1 kappa mouse immunoglobulin that specifically recognizes a 29,000 molecular weight protein (called allergenic protein) with an affinity constant of 1.7 x 10(9) M-1. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ascaris suum; Allergen; IgE; Monoclonal antibodies.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800009

Placental hydroxymethylation vsmethylation at the imprinting control region 2 on chromosome 11p15.5
Provedor de dados: 56
Autores: Magalhães,H.R.; Leite,S.B.P.; Paz,C.C.P. de; Duarte,G.; Ramos,E.S.
In addition to methylated cytosines (5-mCs), hydroxymethylcytosines (5-hmCs) are present in CpG dinucleotide-enriched regions and some transcription regulator binding sites. Unlike methylation, hydroxymethylation does not result in silencing of gene expression, and the most commonly used methods to study methylation, such as techniques based on restriction enzymatic digestion and/or bisulfite modification, are unable to distinguish between them. Genomic imprinting is a process of gene regulation where only one member of an allelic pair is expressed depending on the parental origin. Chromosome 11p15.5 has an imprinting control region (ICR2) that includes a differentially methylated region (KvDMR1) that guarantees parent-specific gene expression. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylation; Hydroxymethylation; Placenta; Genomic Imprinting; ICR2; KvDMR1.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100916

Hereditary angioedema with C1 inhibitor (C1-INH) deficit: the strength of recognition (51 cases)
Provedor de dados: 56
Autores: Fragnan,N.T.M.L.; Tolentino,A.L.N.; Borba,G.B.; Oliveira,A.C.; Simões,J.A.; Palma,S.M.U.; Constantino-Silva,R.N.; Grumach,A.S.
Hereditary angioedema (HAE) is a rare autosomal dominant disease due to C1 esterase inhibitor deficiency (C1-INH). The disease is characterized by subcutaneous and submucosal edema in the absence of urticaria due to the accumulation of bradykinin. This descriptive study aimed to evaluate the clinical characteristics of patients with a confirmed diagnosis of HAE referred to our Outpatient Clinic between December 2009 and November 2017. Fifty-one patients (38 F, 13 M) with a mean age of 32 years (range: 7–70 y) were included. Family history of HAE was reported in 70% (36/51) of the cases; 33/46 patients became symptomatic by 18 years of age. The median time between onset of symptoms and diagnosis was 13 years (3 mo–50 y). The most frequent triggering factors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hereditary angioedema; C1 esterase inhibitor; Diagnosis; Treatment; Complement.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200607

A cost-effective melting temperature assay for the detection of single-nucleotide polymorphism in the MBL2 gene of HIV-1-infected children
Provedor de dados: 56
Autores: Arraes,L.C.; de Souza,P.R.; Bruneska,D.; Castelo Filho,A.; de Souza Cavada,B.; de Lima Filho,J.L.; Crovella,S.
We report a fast (less than 3 h) and cost-effective melting temperature assay method for the detection of single-nucleotide polymorphisms in the MBL2 gene. The protocol, which is based on the Corbett Rotor Gene real time PCR platform and SYBR Green I chemistry, yielded, in the cohorts studied, sensitive (100%) and specific (100%) PCR amplification without the use of costly fluorophore-labeled probes or post-PCR manipulation. At the end of the PCR, the dissociation protocol included a slow heating from 60º to 95ºC in 0.2ºC steps, with an 8-s interval between steps. Melting curve profiles were obtained using the dissociation software of the Rotor Gene-3000 apparatus. Samples were analyzed in duplicate and in different PCR runs to test the reproducibility of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Melting temperature assay; Real time PCR; MBL2; Polymorphisms; HIV-1-positive children.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600003

Factors associated with Clostridium difficile diarrhea in a hospital in Beijing, China
Provedor de dados: 56
Autores: Lv,Z.; Peng,G.L.; Su,J.R.
Clostridium difficile is the most common cause of hospital-acquired diarrhea in patients treated with antibiotics, chemotherapeutic agents, and other drugs that alter the normal equilibrium of the intestinal flora. A better understanding of the risk factors for C. difficile-associated disease (CDAD) could be used to reduce the incidence of CDAD and the costs associated with its treatment. The aim of this study was to identify the risk factors for CDAD in a cohort of Chinese patients in a Beijing hospital. Medical charts of a total of 130 inpatients (62 males and 68 females) with hospital-acquired diarrhea (45 with CDAD; 85 without CDAD) were retrospectively reviewed. C. difficile toxins A and B were detected in fecal samples using enzyme-linked...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotics; Clostridium difficile; Glycopeptides.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201085

TNF-a and IL-10 levels in tracheobronchial lavage of ventilated preterm infants and subsequent lung function
Provedor de dados: 56
Autores: Corso,A.L.; Pitrez,P.M.C.; Machado,D.C.; Stein,R.T.; Jones,M.H.
The role of airway inflammation in ventilated preterm newborns and the risk factors associated with the development of chronic lung disease are not well understood. Our objective was to analyze the association of the airway inflammatory response in ventilated preterm infants by serial measurements of TNF-a and IL-10 in tracheobronchial lavage (TBL) with perinatal factors and lung function measured early in life. A series of TBL samples were collected from ventilated preterm infants (less than 32 weeks of gestational age) and concentrations of TNF-a and IL-10 were measured by ELISA. Pulmonary function tests were performed after discharge by the raised volume rapid compression technique. Twenty-five subjects were recruited and 70 TBL samples were obtained....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Newborn; Chronic lung disease; Interleukin-10; Tumor necrosis factor-a; Prematurity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400016

Transgenic animal models for the functional analysis of vasoactive peptides
Provedor de dados: 56
Autores: Bader,M.
The interplay of vasoactive peptide systems is an essential determinant of blood pressure regulation in mammals. While the endothelin and the renin-angiotensin systems raise blood pressure by inducing vasoconstriction and sodium retention, the kallikrein-kinin and the natriuretic-peptide systems reduce arterial pressure by eliciting vasodilatation and natriuresis. Transgenic technology has proven to be very useful for the functional analysis of vasoactive peptide systems. As an outstanding example, transgenic rats overexpressing the mouse Ren-2 renin gene in several tissues become extremely hypertensive. Several other transgenic rat and mouse strains with genetic modifications of components of the renin-angiotensin system have been developed in the past...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin-angiotensin system; Transgenic; Animal models; Knockout.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900011

Thiol-independent activity of a cholesterol-binding enterohemolysin produced by enteropathogenic Escherichia coli
Provedor de dados: 56
Autores: Figueirêdo,P.M.S.; Catani,C.F.; Yano,T.
Enterohemolysin produced by Escherichia coli associated with infant diarrhea showed characteristics similar to those of thiol-activated hemolysins produced by Gram-positive bacteria, including inactivation by cholesterol, lytic activity towards eukaryotic cells and thermoinstability. However, enterohemolysin activity was not inactivated by oxidation or by SH group-blocking agents (1 mM HgCl2, 1 mM iodoacetic acid) and the hemolysin (100 µg/ml) was not lethal to mice, in contrast to the lethality of the thiol-activated hemolysin family to animals. Earlier reports showed that intravenous injection of partially purified streptolysin O preparations (0.2 µg) was rapidly lethal to mice. These results suggest that E. coli enterohemolysin is not a thiol-activated...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Escherichia coli; Enterohemolysin; Thiol-independent activity; Cholesterol binding.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100008

Lamivudine therapy for hepatitis B in renal transplantation
Provedor de dados: 56
Autores: Santos,F.R.L.; Haiashi,A.R.; Araújo,M.R.T.; Abensur,H.; Romão Junior,J.E.; Noronha,I.L.
Antiviral therapies are associated with an increased risk of acute rejection in transplant patients. The aim of the present study was to evaluate the efficacy and safety of lamivudine therapy for hepatitis B virus (HBV) infection in renal transplant patients. Six patients were included in this study. They received 150 mg/day of lamivudine during a follow-up period of 24 months. The laboratory tests monitored were HBV DNA, HBsAg, HBeAg, ALT, gamma-GT, serum creatinine and blood cyclosporine levels. The HBV DNA became undetectable in four patients as early as in the third month of treatment. After six months, the viral load was also negative in the other two patients, and remained so until 18 months of follow-up. The medication was well tolerated with no...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lamivudine; Kidney transplantation; Hepatitis B.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200008

Salt and insulin sensitivity after short and prolonged high salt intake in elderly subjects
Provedor de dados: 56
Autores: Lima,N.K.C.; Tozetto,D.J.O.; Lima,L.G.; Nobre,F.; Moriguti,J.C.; Ferriolli,E.; Foss,M.C.
Salt sensitivity and insulin resistance are correlated with higher cardiovascular risk. There is no information about changes in salt sensitivity (SS) and insulin sensitivity (IS) after a chronic salt overload in humans. The aim of this study was to evaluate these parameters in the elderly. Seventeen volunteers aged 70.5 ± 5.9 years followed a low-salt diet (LSD) for 1 week and a high-salt diet (HSD) for 13 weeks. We evaluated SS after one week (HSD1) and after 13 weeks (HSD13), and subjects’ IS and lipids on their usual diet (UD) at HSD1, and at HSD13. Blood pressure (BP) was measured at each visit and ambulatory blood pressure monitoring (ABPM) was performed twice. SS was the same at HSD1 and HSD13. Systolic BP was lower on LSD than on UD (P = 0.01),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salt sensitivity; Insulin sensitivity; Elderly; Ambulatory blood pressure monitoring; Blood pressure.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800008

Participation of the NO/cGMP/K+ATP pathway in the antinociception induced by Walker tumor bearing in rats
Provedor de dados: 56
Autores: Barbosa,A.L.R.; Pinheiro,C.A.; Oliveira,G.J.; Torres,J.N.L.; Moraes,M.O.; Ribeiro,R.A.; Vale,M.L.; Souza,M.H.L.P.
Implantation of Walker 256 tumor decreases acute systemic inflammation in rats. Inflammatory hyperalgesia is one of the most important events of acute inflammation. The L-arginine/NO/cGMP/K+ATP pathway has been proposed as the mechanism of peripheral antinociception mediated by several drugs and physical exercise. The objective of this study was to investigate a possible involvement of the NO/cGMP/K+ATP pathway in antinociception induced in Walker 256 tumor-bearing male Wistar rats (180-220 g). The groups consisted of 5-6 animals. Mechanical inflammatory hypernociception was evaluated using an electronic version of the von Frey test. Walker tumor (4th and 7th day post-implantation) reduced prostaglandin E2- (PGE2, 400 ng/paw; 50 µL; intraplantar injection)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Walker 246 tumor-bearing rats; Hypernociception; Nitric oxide; Carrageenan.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600009

Long-term habituation (LTH) in the crab Chasmagnathus: a model for behavioral and mechanistic studies of memory
Provedor de dados: 56
A decade of studies on long-term habituation (LTH) in the crab Chasmagnathus is reviewed. Upon sudden presentation of a passing object overhead, the crab reacts with an escape response that habituates promptly and for at least five days. LTH proved to be an instance of associative memory and showed context, stimulus frequency and circadian phase specificity. A strong training protocol (STP) (&lt;FONT FACE="Symbol"&gt;³&lt;/font&gt;15 trials, intertrial interval (ITI) of 171 s) invariably yielded LTH, while a weak training protocol (WTP) (&lt;FONT FACE="Symbol"&gt;£&lt;/font&gt;10 trials, ITI = 171 s) invariably failed. STP was used with a presumably amnestic agent and WTP with a presumably hypermnestic agent. Remarkably, systemic administration of low...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Memory; Massed training; Spaced training; Cycloheximide; PKA; Angiotensin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700001

Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1α expression
Provedor de dados: 56
Autores: Wang,T.; Zhou,Y.T.; Chen,X.N.; Zhu,A.X.
Hypoxia-inducible factor-1α (HIF-1α) is one of the most potent angiogenic growth factors. It improves angiogenesis and tissue perfusion in ischemic skeletal muscle. In the present study, we tested the hypothesis that ischemic postconditioning is effective for salvaging ischemic skeletal muscle resulting from limb ischemia-reperfusion injury, and that the mechanism involves expression of HIF-1α. Wistar rats were randomly divided into three groups (n=36 each): sham-operated (group S), hindlimb ischemia-reperfusion (group IR), and ischemic postconditioning (group IPO). Each group was divided into subgroups (n=6) according to reperfusion time: immediate (0 h, T0), 1 h (T1), 3 h (T3), 6 h (T6), 12 h (T12), and 24 h (T24). In the IPO group, three cycles of 30-s...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypoxia-inducible factor-1α; Ischemic postconditioning; Skeletal muscle ischemia.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900738

Time course of changes in heart rate and blood pressure variability in rats with myocardial infarction
Provedor de dados: 56
Autores: Aires,R.; Pimentel,E.B.; Forechi,L.; Dantas,E.M.; Mill,J.G.
Our aim was to determine the time course of changes in autonomic balance in the acute (1 and 3 days), sub-acute (7 days) and chronic (28 days) phases of myocardial infarction (MI) in rats. Autonomic balance was assessed by temporal and spectral analyses of blood pressure variability (BPV) and heart rate variability (HRV). Pulsatile blood pressure (BP) recordings (30 min) were obtained in awake and unrestrained male Wistar rats (N = 77; 8-10 weeks old) with MI (coronary ligature) or sham operation (SO). Data are reported as means±SE. The high frequency (HF) component (n.u.) of HRV was significantly lower in MI-1- (P&lt;0.01) and MI-3-day rats (P&lt;0.05) than in their time-control groups (SO-1=68±4 vs MI-1=35.3±4.3; SO-3=71±5.8 vs MI-3=45.2±3.8), without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial infarction; Heart rate variability; Blood pressure variability; Autonomic nervous system; Heart failure.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100605

NMDA receptor blockade alters the intracellular distribution of neuronal nitric oxide synthase in the superficial layers of the rat superior colliculus
Provedor de dados: 56
Autores: Bittencourt-Navarrete,R.E. de; Nascimento,I.C.do; Santiago,M.F.; Mendez-Otero,R.
Nitric oxide (NO) is a molecular messenger involved in several events of synaptic plasticity in the central nervous system. Ca2+ influx through the N-methyl-D-aspartate receptor (NMDAR) triggers the synthesis of NO by activating the enzyme neuronal nitric oxide synthase (nNOS) in postsynaptic densities. Therefore, NMDAR and nNOS are part of the intricate scenario of postsynaptic densities. In the present study, we hypothesized that the intracellular distribution of nNOS in the neurons of superior colliculus (SC) superficial layers is an NMDAR activity-dependent process. We used osmotic minipumps to promote chronic blockade of the receptors with the pharmacological agent MK-801 in the SC of 7 adult rats. The effective blockade of NMDAR was assessed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superior colliculus; Nitric oxide synthase; NMDA receptor; Postsynaptic density targeting.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200007

Early myelin breakdown following sural nerve crush: a freeze-fracture study
Provedor de dados: 56
Autores: Martinez,A.M.B.; Canavarro,S.
In this study we describe the early changes of the myelin sheath following surgical nerve crush. We used the freeze-fracture technique to better evaluate myelin alterations during an early stage of Wallerian degeneration. Rat sural nerves were experimentally crushed and animals were sacrificed by transcardiac perfusion 30 h after surgery. Segments of the nerves were processed for routine transmission electron microscopy and freeze-fracture techniques. Our results show that 30 h after the lesion there was asynchrony in the pattern of Wallerian degeneration, with different nerve fibers exhibiting variable degrees of axon disruption. This was observed by both techniques. Careful examination of several replicas revealed early changes in myelin membranes...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myelin breakdown; Wallerian degeneration; Freeze-fracture; Crush lesion; Sural nerve.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200012

Trans fatty acid intake is associated with insulin sensitivity but independently of inflammation
Provedor de dados: 56
Autores: Angelieri,C.T.; Barros,C.R.; Siqueira-Catania,A.; Ferreira,S.R.G.
High saturated and trans fatty acid intake, the typical dietary pattern of Western populations, favors a proinflammatory status that contributes to generating insulin resistance (IR). We examined whether the consumption of these fatty acids was associated with IR and inflammatory markers. In this cross-sectional study, 127 non-diabetic individuals were allocated to a group without IR and 56 to another with IR, defined as homeostasis model assessment-IR (HOMA-IR) &amp;gt;2.71. Diet was assessed using 24-h food recalls. Multiple linear regression was employed to test independent associations with HOMA-IR. The IR group presented worse anthropometric, biochemical and inflammatory profiles. Energy intake was correlated with abdominal circumference and inversely...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin resistance; Inflammation; Fatty acids.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700009

Origin and pathogenesis of antiphospholipid antibodies
Provedor de dados: 56
Autores: Celli,C.M.; Gharavi,A.E.
Antiphospholipid antibodies (aPL) are a heterogeneous group of antibodies that are detected in the serum of patients with a variety of conditions, including autoimmune (systemic lupus erythematosus), infectious (syphilis, AIDS) and lymphoproliferative disorders (paraproteinemia, myeloma, lymphocytic leukemias). Thrombosis, thrombocytopenia, recurrent fetal loss and other clinical complications are currently associated with a subgroup of aPL designating the antiphospholipid syndrome. In contrast, aPL from patients with infectious disorders are not associated with any clinical manifestation. These findings led to increased interest in the origin and pathogenesis of aPL. Here we present the clinical features of the antiphospholipid syndrome and review the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiphospholipid antibodies; Antiphospholipid syndrome; Systemic lupus erythematosus; Syphilis; Cardiolipin; Beta-2-glycoprotein I.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600002

Heterologous protein secretion in Lactococcus lactis: a novel antigen delivery system
Provedor de dados: 56
Autores: Langella,P.; Le Loir,Y.
Lactic acid bacteria (LAB) are Gram-positive bacteria and are generally regarded as safe (GRAS) organisms. Therefore, LAB could be used for heterologous protein secretion and they are good potential candidates as antigen delivery vehicles. To develop such live vaccines, a better control of protein secretion is required. We developed an efficient secretion system in the model LAB, Lactococcus lactis. Staphylococcal nuclease (Nuc) was used as the reporter protein. We first observed that the quantity of secreted Nuc correlated with the copy number of the cloning vector. The nuc gene was cloned on a high-copy number cloning vector and no perturbation of the metabolism of the secreting strain was observed. Replacement of nuc native promoter by a strong...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein secretion; Staphylococcal nuclease; Lactococcus lactis; Propeptide; Bovine coronavirus; Epitope.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200007

Efficacy, acceptability, and tolerability of antidepressant treatments for patients with post-stroke depression: a network meta-analysis
Provedor de dados: 56
Autores: Qin,B.; Chen,H.; Gao,W.; Zhao,L.B.; Zhao,M.J.; Qin,H.X.; Chen,W.; Chen,L.; Yang,M.X.
The aim of this study was to investigate the efficacy, acceptability, and tolerability of antidepressants in treating post-stroke depression (PSD) by performing a network meta-analysis of randomized controlled trials of the current literature. Eligible studies were retrieved from online databases, and relevant data were extracted. The primary outcome was efficacy as measured by the mean change in overall depressive symptoms. Secondary outcomes included discontinued treatment for any reason and specifically due to adverse events. Fourteen trials were eligible, which included 949 participants and 9 antidepressant treatments. Few significant differences were found for all outcomes. For the primary outcome, doxepin, paroxetine, and nortriptyline were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antidepressants; Post-stroke depression; Randomized controlled trials; Network meta-analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700601

Immunohistochemical characterization of the chick marginal retina
Provedor de dados: 56
Autores: Lima,I.P.; Saito,K.; Hamassaki,D.E.; Yan,C.Y.I.
The retina is a highly differentiated tissue with a complex layered structure that has been extensively characterized. However, most of the previous studies focused on the histology of the central retina while little is known about the cellular composition, organization and function of the marginal retina. Recent research has identified a subpopulation of multipotential progenitor cells in the marginal regions of the retina, closest to the ciliary body ("ciliary marginal zone"). These cells are capable of differentiation in response to an appropriate stimulus. Thus, it is possible that the structure and composition of the marginal retina are distinct from those of the central retina to accommodate the potential addition of newly formed neurons. To...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marginal retina; Ciliary marginal zone; Calbindin; Calretinin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100006

Weight of preterm newborns during the first twelve weeks of life
Provedor de dados: 56
Autores: Anchieta,L.M.; Xavier,C.C.; Colosimo,E.A.; Souza,M.F.
A longitudinal and prospective study was carried out at two state-operated maternity hospitals in Belo Horizonte during 1996 in order to assess the weight of preterm appropriate-for-gestational-age newborns during the first twelve weeks of life. Two hundred and sixty appropriate-for-gestational-age preterm infants with birth weight &lt;2500 g were evaluated weekly. The infants were divided into groups based on birth weight at 250-g intervals. Using weight means, somatic growth curves were constructed and adjusted to Count's model. Absolute (g/day) and relative (g kg-1 day-1) velocity curves were obtained from a derivative of this model. The growth curve was characterized by weight loss during the 1st week (4-6 days) ranging from 5.9 to 13.3% (the greater...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Premature infants; Growth curves; Growth velocity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600012

Minimal residual disease detection in Tunisian B-acute lymphoblastic leukemia based on immunoglobulin gene rearrangements
Provedor de dados: 56
Autores: Besbes,S.; Hamadou,W.S.; Boulland,M.L.; Youssef,Y.B.; Achour,B.; Regaieg,H.; Khelif,A.; Fest,T.; Soua,Z.
IGH gene rearrangement and IGK-Kde gene deletion can be used as molecular markers for the assessment of B lineage acute lymphoblastic leukemia (B-ALL). Minimal residual disease detected based on those markers is currently the most reliable prognosis factor in B-ALL. The aim of this study was to use clonal IGH/IGK-Kde gene rearrangements to confirm B-ALL diagnosis and to evaluate the treatment outcome of Tunisian leukemic patients by monitoring the minimal residual disease (MRD) after induction chemotherapy. Seventeen consecutive newly diagnosed B-ALL patients were investigated by multiplex PCR assay and real time quantitative PCR according to BIOMED 2 conditions. The vast majority of clonal VH-JH rearrangements included VH3 gene. For IGK deletion, clonal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IGH gene; IGK gene; B-ALL minimal residual disease (MRD); RQ-PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100703

Effect of Lactobacillus delbrueckii on cholesterol metabolism in germ-free mice and on atherogenesis in apolipoprotein E knock-out mice
Provedor de dados: 56
Autores: Portugal,L.R.; Gonçalves,J.L.; Fernandes,L.R.; Silva,H.P.S.; Arantes,R.M.E.; Nicoli,J.R.; Vieira,L.Q.; Alvarez-Leite,J.I.
Elevated blood cholesterol is an important risk factor associated with atherosclerosis and coronary heart disease. Several studies have reported a decrease in serum cholesterol during the consumption of large doses of fermented dairy products or lactobacillus strains. The proposed mechanism for this effect is the removal or assimilation of intestinal cholesterol by the bacteria, reducing cholesterol absorption. Although this effect was demonstrated in vitro, its relevance in vivo is still controversial. Furthermore, few studies have investigated the role of lactobacilli in atherogenesis. The aim of the present study was to determine the effect of Lactobacillus delbrueckii on cholesterol metabolism in germ-free mice and the possible hypocholesterolemic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus; Atherosclerosis; Apolipoprotein E; Cholesterol; Germ-free mice.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500010

Social condition affects hormone secretion and exploratory behavior in rats
Provedor de dados: 56
Autores: Genaro,G.; Schmidek,W.R.; Franci,C.R.
Studies of behavior, endocrinology and physiology have described experiments in which animals housed in groups or in isolation were normally tested individually. The isolation of the animal from its group for testing is perhaps the most common situation used today in experimental procedures, i.e., there is no consideration of the acute stress which occurs when the animal is submitted to a situation different from that it is normally accustomed to, i.e., group living. In the present study, we used 90 male 120-day-old rats (Rattus norvegicus) divided into 5 groups of 18 animals, which were housed 3 per cage, in a total of 6 cages. The animals were tested individually or with their groups for exploratory behavior. Hormones were determined by radioimmunoassay...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolation; Group; Stress; Reproduction; ACTH; Luteinizing hormone; Corticosterone; Prolactin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600008

Angiotensin II-mediated vascular smooth muscle cell growth signaling
Provedor de dados: 56
Autores: Inagami,T.; Eguchi,S.
The mechanism by which Ang II stimulates the growth of vascular smooth muscle cells was investigated by measuring the phosphorylation of mitogen-activated protein kinases ERK 1 and ERK 2. Ca2+ ionophore was found to have effects practically analogous to Ang II. We found that the signaling pathway involves the activation of epidermal growth factor receptor (EGFR) kinase, activation of the adaptor proteins Shc and Grb2, and the small G-protein Ras. Although the mechanism of AT1- (or Ca2+)-induced activation of EGFR is not yet clear, we have found that calcium-dependent protein kinase CAKß/PYK2 and c-Src are involved in this process. These studies indicate a transactivation mechanism that utilizes EGFR as a bridge between a Gq-coupled receptor and activation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; Type 1 receptor; Epidermal growth factor receptor; Vascular smooth muscle cells.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600002

Protective effect of policosanol on atherosclerotic lesions in rabbits with exogenous hypercholesterolemia
Provedor de dados: 56
Autores: Arruzazabala,M.L.; Noa,M.; Menéndez,R.; Más,R.; Carbajal,D.; Valdés,S.; Molina,V.
Policosanol is a mixture of higher aliphatic alcohols purified from sugar cane wax, with cholesterol-lowering effects demonstrable in experimental models and in patients with type II hypercholesterolemia. The protective effects of policosanol on atherosclerotic lesions experimentally induced by lipofundin in rabbits and rats and spontaneously developed in stumptail monkeys have been described. The present study was conducted to determine whether policosanol administered orally to rabbits with exogenous hypercholesterolemia also protects against the development of atherosclerotic lesions. Male New Zealand rabbits weighing 1.5 to 2 kg were randomly divided into three experimental groups which received 25 or 200 mg/kg policosanol (N = 7) orally for 60 days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Policosanol; Atherosclerotic lesions; Exogenous hypercholesterolemia; Rabbits.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700015

Amifostine (WR-2721), a cytoprotective agent during high-dose cyclophosphamide treatment of non-Hodgkin's lymphomas: a phase II study
Provedor de dados: 56
Autores: De Souza,C.A.; Santini,G.; Marino,G.; Nati,S.; Congiu,A.M.; Vigorito,A.C.; Damasio,E.
Clinical trials indicate that amifostine may confer protection on various normal tissues without attenuating anti-tumor response. When administered prior to chemotherapy or radiotherapy, it may provide a broad spectrum of cytoprotection including against alkylating drugs. The mechanism of protection resides in the metabolism at normal tissue site by membrane-bound alkaline phosphatase. Toxicity of this drug is moderate with hypotension, nausea and vomiting, and hypocalcemia being observed. We report a phase II study using amifostine as a protective drug against high-dose cyclophosphamide (HDCY) (7 g/m2), used to mobilize peripheral blood progenitor cells (PBPC) and to reduce tumor burden. We enrolled 29 patients, 22 (75.9%) affected by aggressive and 7...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amifostine; Cytoprotection; Non-Hodgkin's lymphoma; High-dose cyclophosphamide; Peripheral blood progenitor cell mobilization.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700009

Baroreflex function in conscious rats submitted to iron overload
Provedor de dados: 56
Autores: Cardoso,L.M.; Pedrosa,M.L.; Silva,M.E.; Moraes,M.F.D.; Colombari,E.; Chianca-Jr.,D.A.
Our hypothesis is that iron accumulated in tissue, rather than in serum, may compromise cardiovascular control. Male Fischer 344 rats weighing 180 to 220 g were divided into 2 groups. In the serum iron overload group (SIO, N = 12), 20 mg elemental iron was injected ip daily for 7 days. In the tissue iron overload group (TIO, N = 19), a smaller amount of elemental iron was injected (10 mg, daily) for 5 days followed by a resting period of 7 days. Reflex heart rate responses were elicited by iv injections of either phenylephrine (0.5 to 5.0 µg/kg) or sodium nitroprusside (1.0 to 10.0 µg/kg). Baroreflex curves were determined and fitted to sigmoidal equations and the baroreflex gain coefficient was evaluated. To evaluate the role of other than a direct effect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baroreflex; Iron overload; Heart rate; Blood pressure; Iron dextran; Deferoxamine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200008

In vitro modulation of Bcl-2 levels in small cell lung cancer cells: effects on cell viability
Provedor de dados: 56
Autores: Santos,A.O.; Pereira,J.P.; Pedroso de Lima,M.C.; Simões,S.; Moreira,J.N.
Small cell lung cancer (SCLC) is an aggressive disease, representing 15% of all cases of lung cancer, has high metastatic potential and low prognosis that urgently demands the development of novel therapeutic approaches. One of the proposed approaches has been the down-regulation of BCL2, with poorly clarified and controversial therapeutic value regarding SCLC. The use of anti-BCL2 small interfering RNA (siRNA) in SCLC has never been reported. The aim of the present study was to select and test the in vitro efficacy of anti-BCL2 siRNA sequences against the protein and mRNA levels of SCLC cells, and their effects on cytotoxicity and chemosensitization. Two anti-BCL2 siRNAs and the anti-BCL2 G3139 oligodeoxynucleotide (ODN) were evaluated in SCLC cells by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Small cell lung cancer; Gene silencing; Bcl-2; SiRNA; Antisense ODN; Flow cytometry; Chemosensitization.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000013

Effects of microcystin-LR in isolated perfused rat kidney
Provedor de dados: 56
Autores: Nobre,A.C.L.; Jorge,M.C.M.; Menezes,D.B.; Fonteles,M.C.; Monteiro,H.S.A.
Microcystin is a hepatotoxic peptide which inhibits protein phosphatase types 1 and 2A. The objective of the present study was to evaluate the physiopathologic effects of microcystin-LR in isolated perfused rat kidney. Adult Wistar rats (N = 5) of both sexes (240-280 g) were utilized. Microcystin-LR (1 µg/ml) was perfused over a period of 120 min, during which samples of urine and perfusate were collected at 10-min intervals to determine the levels of inulin, sodium, potassium and osmolality. We observed a significant increase in urinary flow with a peak effect at 90 min (control (C) = 0.20 ± 0.01 and treated (T) = 0.32 ± 0.01 ml g-1 min-1, P&amp;lt;0.05). At 90 min there was a significant increase in perfusate pressure (C = 129.7 ± 4.81 and T = 175.0 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcystin-LR; Kidney; Perfused kidney.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800008

Repeatability of the “flash-replenishment” method in contrast-enhanced ultrasound for the quantitative assessment of hepatic microvascular perfusion
Provedor de dados: 56
Autores: Xie,Fang; Wan,Wen-Bo; Fei,Xiang; Zhang,Ming-Bo; Zhang,Yan; Wang,Hong-Wei; Tang,Jie; Tang,Wen-Bo; Luo,Yu-Kun.
This study aimed to evaluate the feasibility and repeatability of the flash-replenishment method in contrast-enhanced ultrasound (CEUS) perfusion imaging and assess quantitatively microvascular perfusion in the liver. Twenty healthy New Zealand rabbits were submitted to CEUS perfusion imaging with continuous intravenous infusion. Using flash-replenishment kinetics, the dynamic process of depletion and refilling of microbubble contrast agent was recorded. The hepatic microvascular perfusion parameters were calculated, including region of interest, peak intensity (PI), area under the curve (AUC), and hepatic artery to vein transit time (HA-HVTT). A consistency test was performed for multiple measurements by the same operator and blind measurements by two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flash-replenishment; Contrast-enhanced ultrasound; Liver ischemia-reperfusion injury; Microvascular perfusion; Rabbits.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400603

Risk factors for decreased bone density in premenopausal women
Provedor de dados: 56
Autores: Krahe,C.; Friedman,R.; Gross,J.L.
Osteoporosis is a major health problem. Little is known about the risk factors in premenopause. Sixty 40-50-year old patients with regular menses were studied cross-sectionally. None of the patients were on drugs known to interfere with bone mass. Patients answered a dietary inquiry and had their bone mineral density (BMD) measured. The Z scores were used for the comparisons. A blood sample was taken for the determination of FSH, SHBG, estradiol, testosterone, calcium and alkaline phosphatase. Calcium and creatinine were measured in 24-h urine. A Z score less than -1 was observed for the lumbar spine of 14 patients (23.3%), and for the femur of 24 patients (40%). Patients with a Z score less than -1 for the lumbar spine were older than patients with a Z...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoporosis; Bone loss; Premenopause; Osteopenia; Densitometry.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900003

Methylmercury inhibits prolactin release in a cell line of pituitary origin
Provedor de dados: 56
Autores: Maués,L.A.L.; Macchi,B.M.; Crespo-López,M.E.; Nasciutti,L.E.; Picanço-Diniz,D.L.W.; Antunes-Rodrigues,J.; Nascimento,J.L.M. do.
Heavy metals, such as methylmercury, are key environmental pollutants that easily reach human beings by bioaccumulation through the food chain. Several reports have demonstrated that endocrine organs, and especially the pituitary gland, are potential targets for mercury accumulation; however, the effects on the regulation of hormonal release are unclear. It has been suggested that serum prolactin could represent a biomarker of heavy metal exposure. The aim of this study was to evaluate the effect of methylmercury on prolactin release and the role of the nitrergic system using prolactin secretory cells (the mammosomatotroph cell line, GH3B6). Exposure to methylmercury (0-100 μM) was cytotoxic in a time- and concentration-dependent manner, with an LC50...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury; Methylmercury; Prolactin; Oxidative stress; Pituitary; Nitric oxide.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800691

Adeno-associated virus for cystic fibrosis gene therapy
Provedor de dados: 56
Autores: Martini,S.V.; Rocco,P.R.M.; Morales,M.M.
Gene therapy is an alternative treatment for genetic lung disease, especially monogenic disorders such as cystic fibrosis. Cystic fibrosis is a severe autosomal recessive disease affecting one in 2500 live births in the white population, caused by mutation of the cystic fibrosis transmembrane conductance regulator (CFTR). The disease is classically characterized by pancreatic enzyme insufficiency, an increased concentration of chloride in sweat, and varying severity of chronic obstructive lung disease. Currently, the greatest challenge for gene therapy is finding an ideal vector to deliver the transgene (CFTR) to the affected organ (lung). Adeno-associated virus is the most promising viral vector system for the treatment of respiratory disease because it...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene therapy; Adeno-associated virus; Cystic fibrosis; Cystic fibrosis transmembrane conductance regulator; Vectors.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100004

Texture analysis of computed tomography images of acute ischemic stroke patients
Provedor de dados: 56
Autores: Oliveira,M.S.; Fernandes,P.T.; Avelar,W.M.; Santos,S.L.M.; Castellano,G.; Li,L.M.
Computed tomography (CT) images are routinely used to assess ischemic brain stroke in the acute phase. They can provide important clues about whether to treat the patient by thrombolysis with tissue plasminogen activator. However, in the acute phase, the lesions may be difficult to detect in the images using standard visual analysis. The objective of the present study was to determine if texture analysis techniques applied to CT images of stroke patients could differentiate between normal tissue and affected areas that usually go unperceived under visual analysis. We performed a pilot study in which texture analysis, based on the gray level co-occurrence matrix, was applied to the CT brain images of 5 patients and of 5 control subjects and the results were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Texture analysis; Computed tomography; Thrombolysis; Discriminant analysis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100012

Acute myeloid leukemia in elderly patients: experience of a single center
Provedor de dados: 56
Autores: Rodrigues,C.A.; Chauffaille,M.L.L.F.; Pelloso,L.A.F.; Ghaname,F.S.; Kerbauy,D.M.B.; Campos,M.G.V.; Yamamoto,M.
Acute myeloid leukemia (AML) is a disease predominantly of older adults. Treatment of AML in the elderly is complicated not only by comorbidities but also by the high prevalence of poor prognosis markers. Thirty-one consecutive unselected patients with AML older than 60 years (representing 33% of all AML cases diagnosed at our institution during the same period) were followed over a period of 5 years (1997-2002). A high incidence of AML with multilineage dysplasia (45%) and no favorable cytogenetic abnormalities but 62% intermediate and 38% unfavorable karyotypes were found. Sixteen patients (52%) were selected for induction of intensive cytotoxic treatment and complete remission was achieved only by some of these intensively treated patients (7 of 16). Of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myeloid leukemia; Elderly patients; G-banding karyotype; Induction therapy; Overall survival.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600004

A pilot study on the identification of human papillomavirus genotypes in tongue cancer samples from a single institution in Ecuador
Provedor de dados: 56
Autores: Delgado Ramos,G.M.; Cotter,T.G.; Flor Ramos,L.; Torres Floril,V.; Ramos Martinez,G.A.; Ruiz-Cabezas,J.C.
The relationship between human papillomavirus (HPV) and oropharyngeal squamous cell carcinoma has been established. However, data from Ecuador is limited. The objective of this study was to characterize HPV infection in Ecuadorian patients with tongue cancer. Fifty-three patients with tongue cancer treated at the tertiary referral center Sociedad de Lucha Contra el Cancer (SOLCA), Guayaquil, between 2006 and 2011 were identified. Linear Array® HPV genotyping was used to identify the presence and types of HPV on formalin-fixed paraffin-embedded biopsy samples from these patients with tongue cancer. HPV was identified in 42% (n=22) and high-risk (HR) HPV in 17% (n=9), with 18 different HPV types identified. The most common types were the HR HPV 33 (14%) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Oropharyngeal; Oral; Ecuador; Head and neck cancer; Epidemiology.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100609

The superoxide anion donor, potassium superoxide, induces pain and inflammation in mice through production of reactive oxygen species and cyclooxygenase-2
Provedor de dados: 56
Autores: Maioli,N.A.; Zarpelon,A.C.; Mizokami,S.S.; Calixto-Campos,C.; Guazelli,C.F.S.; Hohmann,M.S.N.; Pinho-Ribeiro,F.A.; Carvalho,T.T.; Manchope,M.F.; Ferraz,C.R.; Casagrande,R.; Verri Jr,W.A.
It is currently accepted that superoxide anion (O2•−) is an important mediator in pain and inflammation. The role of superoxide anion in pain and inflammation has been mainly determined indirectly by modulating its production and inactivation. Direct evidence using potassium superoxide (KO2), a superoxide anion donor, demonstrated that it induced thermal hyperalgesia, as assessed by the Hargreaves method. However, it remains to be determined whether KO2 is capable of inducing other inflammatory and nociceptive responses attributed to superoxide anion. Therefore, in the present study, we investigated the nociceptive and inflammatory effects of KO2. The KO2-induced inflammatory responses evaluated in mice were: mechanical hyperalgesia (electronic version of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superoxide anion; Potassium superoxide; Pain; Inflammation; Oxidative stress.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400321

Adrenocortical tumors in children
Provedor de dados: 56
Autores: Ribeiro,R.C.; Michalkiewicz,E.L.; Figueiredo,B.C.; DeLacerda,L.; Sandrini,F.; Pianovsky,M.D.; Sampaio,G.; Sandrini,R.
Childhood adrenocortical tumors (ACT) are rare. In the USA, only about 25 new cases occur each year. In Southern Brazil, however, approximately 10 times that many cases are diagnosed each year. Most cases occur in the contiguous states of São Paulo and Paraná. The cause of this higher rate has not been identified. Familial genetic predisposition to cancer (p53 mutations) and selected genetic syndromes (Beckwith-Wiedemann syndrome) have been associated with childhood ACT in general but not with the Brazilian counterpart. Most of the affected children are young girls with classic endocrine syndromes (virilizing and/or Cushing). Levels of urinary 17-ketosteroids and plasma dehydroepiandrosterone sulfate (DHEA-S), which are abnormal in approximately 90% of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenocortical adenoma; Adrenocortical carcinoma; Adrenal gland disorders; Pediatric cancers; Childhood adrenocortical tumors.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000013

Effects of different frequencies of transcutaneous electrical nerve stimulation on venous vascular reactivity
Provedor de dados: 56
Autores: Franco,O.S.; Paulitsch,F.S.; Pereira,A.P.C.; Teixeira,A.O.; Martins,C.N.; Silva,A.M.V.; Plentz,R.D.M.; Irigoyen,M.C.; Signori,L.U.
Transcutaneous electrical nerve stimulation (TENS) is a type of therapy used primarily for analgesia, but also presents changes in the cardiovascular system responses; its effects are dependent upon application parameters. Alterations to the cardiovascular system suggest that TENS may modify venous vascular response. The objective of this study was to evaluate the effects of TENS at different frequencies (10 and 100 Hz) on venous vascular reactivity in healthy subjects. Twenty-nine healthy male volunteers were randomized into three groups: placebo (n=10), low-frequency TENS (10 Hz, n=9) and high-frequency TENS (100 Hz, n=10). TENS was applied for 30 min in the nervous plexus trajectory from the superior member (from cervical to dorsal region of the fist)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electric stimulation therapy; Vascular endothelium; Alpha-1 adrenergic receptors; Mechanical pain.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500411

Induction of oral tolerance and the effect of interleukin-4 on murine skin allograft rejection
Provedor de dados: 56
Autores: Dettino,A.L.A.; Duarte,A.J.S.; Sato,M.N.
We studied the effect of oral and portal vein administration of alloantigens on mouse skin allograft survival. Graft receptor BALB/c mice received spleen cells (30, 90, 150 or 375 x 10(6)) from donor C57BL/6 mice intragastrically on three successive days, starting seven days before the skin graft. Allograft survival was significantly increased with the feeding of 150 x 10(6) allogeneic spleen cells by one gavage (median survival of 12 vs 14 days, P &lt;= 0.005) or when 300 x 10(6) cells were given in six gavage (12 vs 14 days, P &lt; 0.04). A similar effect was observed when 150 x 10(6) spleen cells were injected into the portal vein (12 vs 14 days, P &lt;= 0.03). Furthermore, prolonged allograft survival was observed with subcutaneous (12 vs 16 days, P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mouse oral tolerance; Intraportal route; Skin transplantation; Interleukin-4; Alloantigen.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300022

Adhesion molecule profiles of B-cell non-Hodgkin's lymphomas in the leukemic phase
Provedor de dados: 56
Autores: Matos,D.M.; Rizzatti,E.G.; Garcia,A.B.; Gallo,D.A.P.; Falcão,R.P.
We evaluated the expression of 10 adhesion molecules on peripheral blood tumor cells of 17 patients with chronic lymphocytic leukemia, 17 with mantle-cell lymphoma, and 13 with nodal or splenic marginal B-cell lymphoma, all in the leukemic phase and before the beginning of any therapy. The diagnosis of B-cell non-Hodgkin's lymphomas was based on cytological, histological, immunophenotypic, and molecular biology methods. The mean fluorescence intensity of the adhesion molecules in tumor cells was measured by flow cytometry of CD19-positive cells and differed amongst the types of lymphomas. Comparison of chronic lymphocytic leukemia and mantle-cell lymphoma showed that the former presented a higher expression of CD11c and CD49c, and a lower expression of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-Hodgkin's lymphoma; Adhesion molecules; Flow cytometry; CD49c/CD49d.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001000011

Distribution of 131I-labeled Bothrops erythromelas venom in mice
Provedor de dados: 56
Autores: Vasconcelos,C.M.L.; Valença,R.C.; Araújo,E.A.; Modesto,J.C.A.; Pontes,M.M.; Brazil,T.K.; Guarnieri,M.C.
Bothrops erythromelas is responsible for many snake bites in northeastern Brazil. In the present study we determined the in vivo distribution of the venom following its subcutaneous injection into mice. B. erythromelas venom and albumin were labeled individually with 131I by the chloramine T method, and separated in a Sephacryl® S-200 column. The efficiency of labeling was 68%. Male Swiss mice (40-45 g), which had been provided with drinking water containing 0.05% KI over a period of 10 days prior to the experiment, were inoculated dorsally (sc) with 0.3 ml (2.35 x 105 cpm/mouse) of 131I-venom (N = 42), 131I-albumin or 131I (controls, N = 28 each). Thirty minutes and 1, 3, 6, 12, 18 and 24 h after inoculation, the animals were perfused with 0.85% NaCl and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops erythromelas; Snake venom; Protein iodination.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300017

Protective effects of tumor necrosis factor-α blockade by adalimumab on articular cartilage and subchondral bone in a rat model of osteoarthritis
Provedor de dados: 56
Autores: Ma,C.H.; Lv,Q.; Yu,Y.X.; Zhang,Y.; Kong,D.; Niu,K.R.; Yi,C.Q.
We aimed to investigate the effects of an anti-tumor necrosis factor-α antibody (ATNF) on cartilage and subchondral bone in a rat model of osteoarthritis. Twenty-four rats were randomly divided into three groups: sham-operated group (n=8); anterior cruciate ligament transection (ACLT)+normal saline (NS) group (n=8); and ACLT+ATNF group (n=8). The rats in the ACLT+ATNF group received subcutaneous injections of ATNF (20 μg/kg) for 12 weeks, while those in the ACLT+NS group received NS at the same dose for 12 weeks. All rats were euthanized at 12 weeks after surgery and specimens from the affected knees were harvested. Hematoxylin and eosin staining, Masson's trichrome staining, and Mankin score assessment were carried out to evaluate the cartilage status and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-tumor necrosis factor-α antibody (ATNF); Osteoarthrosis; Matrix metalloproteinase (MMP)-13; Articular cartilage; Subchondral bone.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000001

An improved experimental model for peripheral neuropathy in rats
Provedor de dados: 56
Autores: Dias,Q.M.; Rossaneis,A.C.; Fais,R.S.; Prado,W.A.
A modification of the Bennett and Xie chronic constriction injury model of peripheral painful neuropathy was developed in rats. Under tribromoethanol anesthesia, a single ligature with 100% cotton glace thread was placed around the right sciatic nerve proximal to its trifurcation. The change in the hind paw reflex threshold after mechanical stimulation observed with this modified model was compared to the change in threshold observed in rats subjected to the Bennett and Xie or the Kim and Chung spinal ligation models. The mechanical threshold was measured with an automated electronic von Frey apparatus 0, 2, 7, and 14 days after surgery, and this threshold was compared to that measured in sham rats. All injury models produced significant hyperalgesia in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic constriction injury; Chronic pain model; Neuropathy; Peripheral nerve lesion; Spinal nerve ligation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300253

Polymorphism of the aryl-hydrocarbon receptor gene in intron 10 of human cancers
Provedor de dados: 56
Autores: Rocas,M.; Jakubauskiene,E.; Kanopka,A.
Polychlorinated dibenzo-p-dioxins (PCDDs) and related halogenated aromatic hydrocarbons (e.g., PCDFs), often called "dioxins", are ubiquitously present environmental contaminants. Some of them, notably 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), are among the most toxic synthetic compounds known. The biological effects of dioxins are mediated via the aryl hydrocarbon receptor (AhR). Mutations in the AhR transactivation domain are linked to sensitivity to the acute lethality of TCDD. We present here a study of AhR gene polymorphism in normal and cancer human tissues affecting pre-mRNA splicing in the AhR gene-coding transactivation domain region (exon 10, intron 10, exon 11 region), previously shown to be associated with AhR dysfunction. We tested 126 pairs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ah receptor; Polymorphism; Splicing; Cancer; Dioxins.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100006

Are birth weight and maternal smoking during pregnancy associated with malnutrition and excess weight among school age children?
Provedor de dados: 56
Autores: Tomé,F.S.; Cardoso,V.C.; Barbieri,M.A.; Silva,A.A.M. da; Simões,V.M.F.; Garcia,C.A.; Bettiol,H.
In the late 1980's child malnutrition was still prevalent in Brazil, and child obesity was beginning to rise in the richest regions of the country. To assess the extent of the nutritional transition during the period and the influence of birth weight and maternal smoking on the nutritional condition of schoolchildren, we estimated the prevalence of excess weight and malnutrition in a cohort of Brazilian schoolchildren from 1987 to 1989. We calculated the body mass index (BMI) of 8- to 10-year-old schoolchildren born in Ribeirão Preto in 1978/79. We considered children with a BMI &lt;5th percentile (P5) to be malnourished, children with P5³BMI&lt;P85 to be thin and normal, and children with BMI ³P85 to be overweight. We evaluated the association of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Child malnutrition; Child obesity; Body mass index; Schoolchildren; Socioeconomic level.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900009

Striatal and extrastriatal atrophy in Huntington's disease and its relationship with length of the CAG repeat
Provedor de dados: 56
Autores: Ruocco,H.H.; Lopes-Cendes,I.; Li,L.M.; Santos-Silva,M.; Cendes,F.
Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder that affects the striatum most severely. However, except for juvenile forms, relative preservation of the cerebellum has been reported. The objective of the present study was to perform MRI measurements of caudate, putamen, cerebral, and cerebellar volumes and correlate these findings with the length of the CAG repeat and clinical parameters. We evaluated 50 consecutive patients with HD using MRI volumetric measurements and compared them to normal controls. Age at onset of the disease ranged from 4 to 73 years (mean: 43.1 years). The length of the CAG repeat ranged from 40 to 69 (mean: 47.2 CAG). HD patients presented marked atrophy of the caudate and putamen, as well as reduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurodegeneration; Dynamic mutation; Genotype-phenotype correlation; Basal ganglia; Magnetic resonance imaging; Huntington's disease.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800016

Prevalence of celiac disease in Brazilian children of short stature
Provedor de dados: 56
Autores: Queiroz,M.S.; Nery,M.; Cançado,E.L.; Gianella-Neto,D.; Liberman,B.
The aim of the present study was to determine the prevalence of celiac disease in children of short stature and to assess whether some of the routine laboratory examinations performed to determine the cause of short stature could suggest the presence of celiac disease. A total of 106 children of short stature and no gastrointestinal symptoms were studied. An extensive endocrine work-up had been negative for all of them and an additional investigation was performed by measuring the concentration of antiendomysial antibody. Patients who were positive for antiendomysial antibody ( &gt; or = 1:10) or who exhibited IgA deficiency (less than 5 mg/dl) were referred for an endoscopic intestinal biopsy. We detected a pathological titer of antiendomysial IgA in six...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Celiac disease; Short stature; Antiendomysial antibody; Growth failure; Celiac sprue.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100008

Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro
Provedor de dados: 56
Autores: Yang,Xia; Qian,Feng; He,Hai-Yang; Liu,Kai-Jun; Lan,Yuan-Zhi; Ni,Bing; Tian,Yi; Fu,Xiao-Lan; Zhang,Ji; Shen,Zi-Gang; Li,Jian; Yin,Yi; Li,Jin-Tao; Wu,Yu-Zhang.
Thymosin alpha 1 (Tα1) has been shown to have beneficial effects on numerous immune system parameters, but little is known about the effects of Tα1 on patients with gastric carcinoma. The objective of this study was to determine the effect of Tα1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro, and to evaluate its efficacy as an immunoregulatory factor in patients with gastric carcinoma. We compared the effect of Tα1 on the frequency of CD4+ and CD8+ T cells, especially the CD4+CD25+Foxp3+ Tregs in peripheral blood mononuclear cells (PBMCs) from gastric carcinoma patients (N = 35) and healthy donors (N = 22). We also analyzed the changes in the proliferation of PBMCs in response to treatment with Tα1, and examined the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tα1; Gastric carcinoma; Tregs; Th1; Th2; Th17.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100005

Genetic and environmental influences on blood pressure and physical activity: a study of nuclear families from Muzambinho, Brazil
Provedor de dados: 56
Autores: Forjaz,C.L.M.; Bartholomeu,T.; Rezende,J.A.S.; Oliveira,J.A.; Basso,L.; Tani,G.; Prista,A.; Maia,J.A.R.
Blood pressure (BP) and physical activity (PA) levels are inversely associated. Since genetic factors account for the observed variation in each of these traits, it is possible that part of their association may be related to common genetic and/or environmental influences. Thus, this study was designed to estimate the genetic and environmental correlations of BP and PA phenotypes in nuclear families from Muzambinho, Brazil. Families including 236 offspring (6 to 24 years) and their 82 fathers and 122 mothers (24 to 65 years) were evaluated. BP was measured, and total PA (TPA) was assessed by an interview (commuting, occupational, leisure time, and school time PA). Quantitative genetic modeling was used to estimate maximal heritability (h²), and genetic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise; Hypertension; Environmental influence; Heritability; Genetic epidemiology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200023

Classical and recent advances in the treatment of inflammatory bowel diseases
Provedor de dados: 56
Autores: Sales-Campos,H.; Basso,P.J.; Alves,V.B.F.; Fonseca,M.T.C.; Bonfá,G.; Nardini,V.; Cardoso,C.R.B.
Crohn's disease (CD) and ulcerative colitis (UC) are intestinal disorders that comprise the inflammatory bowel diseases (IBD). These disorders have a significant effect on the quality of life of affected patients and the increasing number of IBD cases worldwide is a growing concern. Because of the overall burden of IBD and its multifactorial etiology, efforts have been made to improve the medical management of these inflammatory conditions. The classical therapeutic strategies aim to control the exacerbated host immune response with aminosalicylates, antibiotics, corticosteroids, thiopurines, methotrexate and anti-tumor necrosis factor (TNF) biological agents. Although successful in the treatment of several CD or UC conditions, these drugs have limited...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammatory bowel disease; Crohn's disease; Ulcerative colitis; Treatment; Immune modulation; Cellular therapy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200096

Interaction between repeated restraint stress and concomitant midazolam administration on sweet food ingestion in rats
Provedor de dados: 56
Autores: Silveira,P.P.; Xavier,M.H.; Souza,F.H.; Manoli,L.P.; Rosat,R.M.; Ferreira,M.B.C.; Dalmaz,C.
Emotional changes can influence feeding behavior. Previous studies have shown that chronically stressed animals present increased ingestion of sweet food, an effect reversed by a single dose of diazepam administered before testing the animals. The aim of the present study was to evaluate the response of animals chronically treated with midazolam and/or submitted to repeated restraint stress upon the ingestion of sweet food. Male adult Wistar rats were divided into two groups: controls and exposed to restraint 1 h/day, 5 days/week for 40 days. Both groups were subdivided into two other groups treated or not with midazolam (0.06 mg/ml in their drinking water during the 40-day treatment). The animals were placed in a lighted area in the presence of 10 pellets...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feeding behavior; Chronic stress; Benzodiazepines; Midazolam; Sweet taste; Rats.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100013

Induction of a gloverin-like antimicrobial polypeptide in the sugarcane borer Diatraea saccharalis challenged by septic injury
Provedor de dados: 56
Autores: Silva,J.L.C.; Barbosa,J.F.; Bravo,J.P.; Souza,E.M. de; Huergo,L.F.; Pedrosa,F.O.; Esteves,E.; Daffre,S.; Fernandez,M.A.
Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) is an important pest for Brazilian sugarcane. In the present study, we detected two distinct spots in hemolymph from septic injured larvae (HDs1 and HDs2), which are separated by 2DE gel electrophoresis. Both spots were subjected to in-gel tryptic digestion and MALDI-TOF/TOF analysis, which revealed the sequence VFGTLGSDDSGLFGK present in both HDs1 and HDs2. This sequence had homology and 80% identity with specific Lepidoptera antimicrobial peptides called gloverins. Analyses using the ImageMaster 2D software showed pI 8.94 of the HDs1 spot, which is similar to that described to Hyalophora gloveri gloverin (pI 8.5). Moreover, the 14-kDa molecular mass of the spot HDs1 is compatible to that of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diatraea saccharalis; Lepidoptera; Immune response; Gloverin; Sugarcane borer; Hemolymph.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500003

FGFR1 is an independent prognostic factor and can be regulated by miR-497 in gastric cancer progression
Provedor de dados: 56
Autores: Xie,Gang; Ke,Qi; Ji,Yu Zu; Wang,An-qun; Jing,Meng; Zou,li-li.
Fibroblast growth factor receptor 1 (FGFR1) has been reported in gastric cancer to be a prognostic factor. However, miR-497-targeted FGFR1 has not been explored in the carcinogenesis of gastric cancer. The present study intended to revalidate the prognostic significance of FGFR1 in patients with gastric cancer, and the mechanism of miR-497-regulated FGFR1 was investigated in gastric cancer cell proliferation and apoptosis. The messenger RNA (mRNA) and protein levels were assayed by RT-qPCR and western blotting, respectively. The targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. Cell proliferation was analyzed by CCK-8 assay. Annexin V-FITC/PI staining was used to evaluate the apoptosis in AGS and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FGFR1; MiR-497; Apoptosis; Post-transcriptional; Gastric cancer.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100602

Galantamine protects against lipopolysaccharide-induced acute lung injury in rats
Provedor de dados: 56
Autores: Li,G.; Zhou,CL.; Zhou,QS.; Zou,HD.
Lipopolysaccharide (LPS)-induced endotoxemia triggers the secretion of proinflammatory cytokines and can cause acute lung injury (ALI). The high mobility group box 1 (HMGB1) protein plays an important role as a late mediator of sepsis and ALI. Galantamine (GAL) is a central acetylcholinesterase inhibitor that inhibits the expression of HMGB1. This study evaluated the effects of GAL by measuring levels of inflammatory mediators and observing histopathological features associated with LPS-induced ALI. Sixty 8-10 week old male Sprague-Dawley rats (200-240 g) were randomized into three groups as follows: control group, LPS group (7.5 mg/kg LPS), and LPS+GAL group (5 mg/kg GAL before LPS administration). Histopathological examination of lung specimens obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galantamine; Acute lung injury; Lipopolysaccharide; HMGB1.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200601

Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats
Provedor de dados: 56
Autores: Ribeiro,L.P.; Freitas-Lima,L.C.; Naumann,G.B.; Meyrelles,S.S.; Lunz,W.; Pires,S.F.; Andrade,H.M.; Carnielli,J.B.T.; Figueiredo,S.G.
In the present study, we successfully demonstrated for the first time the existence of cardiac proteomic differences between non-selectively bred rats with distinct intrinsic exercise capacities. A proteomic approach based on two-dimensional gel electrophoresis coupled to mass spectrometry was used to study the left ventricle (LV) tissue proteome of rats with distinct intrinsic exercise capacity. Low running performance (LRP) and high running performance (HRP) rats were categorized by a treadmill exercise test, according to distance run to exhaustion. The running capacity of HRPs was 3.5-fold greater than LRPs. Protein profiling revealed 29 differences between HRP and LRP rats (15 proteins were identified). We detected alterations in components involved in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inborn aerobic capacity; Proteomics; 2-DE; MS; Heart proteins.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300609

Reversal by methylene blue of tetanic fade induced in cats by nitric oxide
Provedor de dados: 56
Autores: Ambiel,C.R.; Alves-Do-Prado,W.
Previous data from our laboratory have indicated that nitric oxide (NO) acting at the presynaptic level increases the amplitude of muscular contraction (AMC) of the phrenic-diaphragm preparations isolated from indirectly stimulated rats, but, by acting at the postsynaptic level, it reduces the AMC when the preparations are directly stimulated. In the present study we investigated the effects induced by NO when tetanic frequencies of stimulation were applied to in vivo preparations (sciatic nerve-anterior tibial muscle of the cat). Intra-arterial injection of NO (0.75-1.5 mg/kg) induced a dose-dependent increase in the Wedensky inhibition produced by high frequencies of stimulation applied to the motor nerve. Intra-arterial administration of 7.2 µg/kg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wedensky inhibition; Nitric oxide; Methylene blue; Skeletal muscle.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300013

The eukaryotic Pso2/Snm1/Artemis proteins and their function as genomic and cellular caretakers
Provedor de dados: 56
Autores: Bonatto,D.; Revers,L.F.; Brendel,M.; Henriques,J.A.P.
DNA double-strand breaks (DSBs) represent a major threat to the genomic stability of eukaryotic cells. DNA repair mechanisms such as non-homologous end joining (NHEJ) are responsible for the maintenance of eukaryotic genomes. Dysfunction of one or more of the many protein complexes that function in NHEJ can lead to sensitivity to DNA damaging agents, apoptosis, genomic instability, and severe combined immunodeficiency. One protein, Pso2p, was shown to participate in the repair of DSBs induced by DNA inter-strand cross-linking (ICL) agents such as cisplatin, nitrogen mustard or photo-activated bi-functional psoralens. The molecular function of Pso2p in DNA repair is unknown, but yeast and mammalian cell line mutants for PSO2 show the same cellular responses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-homologous end joining; Double-strand breaks; V(D)J; PSO2; Artemis; Saccharomyces cerevisiae.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300002

Environmental color affects Nile tilapia reproduction
Provedor de dados: 56
Autores: Volpato,G.L.; Duarte,C.R.A.; Luchiari,A.C.
We investigated the effects of environmental color on the reproductive behavior of Nile tilapia, Oreochromis niloticus. Two environmental colors were tested by covering the aquarium (60 x 60 x 40 cm) with white (12 groups) or blue (13 groups) cellophane and observing reproductive behavior in groups of 2 males (10.27 ± 0.45 cm) and 3 females (10.78 ± 0.45 cm) each. After assignment to the respective environmental color (similar luminosity = 100 to 120 Lux), the animals were observed until reproduction (identified by eggs in the female's mouth) or up to 10 days after the first nest building. Photoperiod was from 6:00 h to 18:00 h every day. Food was offered in excess once a day and water quality was similar among aquaria. Daily observations were made at...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Reproduction; Environmental color; Fish; Nile tilapia; Oreochromis niloticus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400004

Secretory TAT-peptide-mediated protein transduction of LIF receptor α-chain distal cytoplasmic motifs into human myeloid HL-60 cells
Provedor de dados: 56
Autores: Sun,Q.; Xiong,J.; Lu,J.; Xu,S.; Li,Y.; Zhong,X.P.; Gao,G.K.; Liu,H.Q.
The distal cytoplasmic motifs of leukemia inhibitory factor receptor α-chain (LIFRα-CT3) can independently induce intracellular myeloid differentiation in acute myeloid leukemia (AML) cells by gene transfection; however, there are significant limitations in the potential clinical use of these motifs due to liposome-derived genetic modifications. To produce a potentially therapeutic LIFRα-CT3 with cell-permeable activity, we constructed a eukaryotic expression pcDNA3.0-TAT-CT3-cMyc plasmid with a signal peptide (ss) inserted into the N-terminal that codes for an ss-TAT-CT3-cMyc fusion protein. The stable transfection of Chinese hamster ovary (CHO) cells via this vector and subsequent selection by Geneticin resulted in cell lines that express and secrete...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukemia inhibitory factor; TAT-HIV1; Protein transduction domain; Acute myeloid leukemia; LIF receptor.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000005

Assessment of tidal volume and thoracoabdominal motion using volume and flow-oriented incentive spirometers in healthy subjects
Provedor de dados: 56
Autores: Parreira,V.F.; Tomich,G.M.; Britto,R.R.; Sampaio,R.F.
The objective of the present study was to evaluate incentive spirometers using volume- (Coach and Voldyne) and flow-oriented (Triflo II and Respirex) devices. Sixteen healthy subjects, 24 ± 4 years, 62 ± 12 kg, were studied. Respiratory variables were obtained by respiratory inductive plethysmography, with subjects in a semi-reclined position (45º). Tidal volume, respiratory frequency, minute ventilation, inspiratory duty cycle, mean inspiratory flow, and thoracoabdominal motion were measured. Statistical analysis was performed with Kolmogorov-Smirnov test, t-test and ANOVA. Comparison between the Coach and Voldyne devices showed that larger values of tidal volume (1035 ± 268 vs 947 ± 268 ml, P = 0.02) and minute ventilation (9.07 ± 3.61 vs 7.49 ± 2.58...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Incentive spirometry; Respiratory inductive plethysmography; Physiotherapy; Volume-oriented spirometer; Flow-oriented spirometer.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700014

The role of the SLAM-SAP signaling pathway in the modulation of CD4+ T cell responses
Provedor de dados: 56
Autores: Vilar,M.L.L.V.; Frutuoso,M.S.; Arruda,S.M.; Lima,D.M.; Bezerra,C.S.; Pompeu,M.M.L.
The signaling lymphocytic activation molecule (SLAM), present on the surface of hematopoietic cells, can regulate some events of the immune responses. This modulatory action is associated with the capacity of SLAM to interact with an intracytoplasmic adapter, such as SLAM-associated protein (SAP). SLAM is constitutively expressed in most of these cells, is rapidly induced after antigenic or inflammatory stimuli, and participates in the immunological synapse. Defects in the function of the SLAM-SAP pathway contribute to immunological abnormalities, resulting in autoimmune diseases, tumors of the lymphoid tissues and inadequate responses to infectious agents. Initially, the role of SLAM was investigated using an anti-SLAM monoclonal antibody (α-SLAM mAb)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SLAM; SAP; IFN-γ; Immunoreceptor; Immunomodulation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400002

Effect of one stretch a week applied to the immobilized soleus muscle on rat muscle fiber morphology
Provedor de dados: 56
Autores: Gomes,A.R.S.; Coutinho,E.L.; França,C.N.; Polonio,J.; Salvini,T.F.
We determined the effect of stretching applied once a week to the soleus muscle immobilized in the shortened position on muscle fiber morphology. Twenty-six male Wistar rats weighing 269 ± 26 g were divided into three groups. Group I, the left soleus was immobilized in the shortened position for 3 weeks; group II, the soleus was immobilized in the shortened position and stretched once a week for 3 weeks; group III, the soleus was submitted only to stretching once a week for 3 weeks. The medial part of the soleus muscle was frozen for histology and muscle fiber area evaluation and the lateral part was used for the determination of number and length of serial sarcomeres. Soleus muscle submitted only to immobilization showed a reduction in weight (44 ± 6%, P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscle; Stretching; Immobilization; Sarcomere number; Muscle fiber area.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000005

Intercellular contact-dependent survival of human A549, NCI-H596 and NCI-H520 non-small cell lung carcinoma cell lines
Provedor de dados: 56
Autores: Castro,M.A.A.; Schwartsmann,G.; Moreira,J.C.F.
In the present study, we examined the relationship between cell phenotype and cell survival of three human non-small cell lung carcinoma cell lines (A549, NCI-H596 and NCI-H520). Cells in exponential growth at various densities were incubated for 24 h at 37ºC in a 5% CO2 humidified atmosphere and then exposed to UV radiation for 1 min (256 nm, 40 W, source-to-target distance 100 cm). After two days the surviving cells were quantified by sulforhodamine ß staining and DNA fragmentation assay. The differences in UV sensitivity at 60 x 10³ cells/cm² among the cell lines were not related to the proliferative state of the cells but to the extent of intercellular contact. In contrast to A549 and NCI-H596, irradiated NCI-H520 cells presented lower DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-small cell lung carcinoma; Cell density; Intercellular contact; Cell phenotype; UV radiation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800006

Relationship of cyclosporin and sirolimus blood concentrations regarding the incidence and severity of hyperlipidemia after kidney transplantation
Provedor de dados: 56
Autores: Spinelli,G.A.; Felipe,C.R.; Machado,P.G.; Garcia,R.; Casarini,D.E.; Moreira,S.R.; Park,S.I.; Tedesco-Silva Jr.,H.; Medina-Pestana,J.O.
The influence of drug concentrations on the development of persistent posttransplant hyperlipidemia was investigated in 82 patients who received cyclosporin A (CsA) and prednisone plus sirolimus (SRL) (52) or azathioprine (AZA) (30) during the first year after transplantation. Blood levels of CsA and SRL, daily doses of AZA and prednisone, and cholesterol, triglyceride, and glucose concentrations were determined during each visit (pretransplant and 30, 60, 90, 120, 180, and 360 days posttransplant). Persistent hyperlipidemia was defined as one-year average steady-state cholesterol (CavCHOL) or triglyceride (CavTG) concentrations above 240 and 200 mg/dL, respectively. Mean cholesterol and triglyceride concentrations increased after transplantation (P &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperlipidemia; Cholesterol; Triglyceride; Kidney transplantation; Cyclosporin A; Sirolimus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100003

Fasting: a major limitation for resistance exercise training effects in rodents
Provedor de dados: 56
Autores: das Neves,W.; de Oliveira,L.F.; da Silva,R.P.; Alves,C.R.R.; Lancha Jr.,A.H.
Protocols that mimic resistance exercise training (RET) in rodents present several limitations, one of them being the electrical stimulus, which is beyond the physiological context observed in humans. Recently, our group developed a conditioning system device that does not use electric shock to stimulate rats, but includes fasting periods before each RET session. The current study was designed to test whether cumulative fasting periods have some influence on skeletal muscle mass and function. Three sets of male Wistar rats were used in the current study. The first set of rats was submitted to a RET protocol without food restriction. However, rats were not able to perform exercise properly. The second and third sets were then randomly assigned into three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Strength; Physical activity; Hypertrophy; Atrophy; Skeletal muscle wasting.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100602

Hypomagnesemia is a risk factor for nonrecovery of renal function and mortality in AIDS patients with acute kidney injury
Provedor de dados: 56
Autores: Biagioni Santos,M.S.; Seguro,A.C.; Andrade,L.
The objective of the present study was to determine the prevalence of electrolyte disturbances in AIDS patients developing acute kidney injury in the hospital setting, as well as to determine whether such disturbances constitute a risk factor for nephrotoxic and ischemic injury. A prospective, observational cohort study was carried out. Hospitalized AIDS patients were evaluated for age; gender; coinfection with hepatitis; diabetes mellitus; hypertension; time since HIV seroconversion; CD4 count; HIV viral load; proteinuria; serum levels of creatinine, urea, sodium, potassium and magnesium; antiretroviral use; nephrotoxic drug use; sepsis; intensive care unit (ICU) admission, and the need for dialysis. Each of these characteristics was correlated with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acquired immunodeficiency syndrome; Acute kidney failure; Water-electrolyte imbalance; Magnesium deficiency; Drug toxicity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300015

Risk factors for glucose intolerance in active acromegaly
Provedor de dados: 56
Autores: Kreze,A.; Kreze-Spirova,E.; Mikulecky,M.
In the present retrospective study we determined the frequency of glucose intolerance in active untreated acromegaly, and searched for risk factors possibly supporting the emergence of the diabetic condition. Among 43 patients, 8 (19%; 95% CI: 8-33%) had diabetes mellitus and 2 (5%; 1-16%) impaired glucose tolerance. No impaired fasting glycemia was demonstrable. The frequency of diabetes was on average 4.5 times higher than in the general Slovak population. Ten factors suspected to support progression to glucose intolerance were studied by comparing the frequency of glucose intolerance between patients with present and absent risk factors. A family history of diabetes and arterial hypertension proved to have a significant promoting effect (P&lt;0.05,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acromegaly; Diabetes mellitus; Impaired glucose tolerance; Impaired fasting glycemia; Risk factors; Inferential statistics.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100009

Time collection and storage conditions of lipid profile
Provedor de dados: 56
Autores: França,C.N.; Mendes,C.C.; Ferreira,C.E.S.
The stability of samples is crucial for getting reliable concentrations of many analytes, including lipid profile. Thus, the goal of this study was to analyze lipid profile under different storage and temperature conditions. This was a prospective study with 809 patients of both genders. Total cholesterol, triglycerides, high-density lipoprotein cholesterol, low density lipoprotein cholesterol and non-high-density lipoprotein were measured within 1 h from collection at room temperature, after 2-3 h of refrigeration (8°C) and after 4-5 h at room temperature. The processing time and storage conditions did not affect the analytes measured. These findings are important for multicenter studies, because of the difficulties related to centrifugation and freezing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipid profile; Storage; Temperature; Stability; Refrigerator; Room temperature.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300608

Pain-related diseases and sleep disorders
Provedor de dados: 56
Autores: Roizenblatt,M.; Rosa Neto,N.S.; Tufik,S.; Roizenblatt,S.
Pain and sleep share mutual relations under the influence of cognitive and neuroendocrine changes. Sleep is an important homeostatic feature and, when impaired, contributes to the development or worsening of pain-related diseases. The aim of the present review is to provide a panoramic view for the generalist physician on sleep disorders that occur in pain-related diseases within the field of Internal Medicine, such as rheumatic diseases, acute coronary syndrome, digestive diseases, cancer, and headache.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep disorders; Rheumatic diseases; Acute coronary syndrome; Irritable bowel syndrome; Cancer; Headache.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900001

Role of microRNAs in the treatment of type 2 diabetes mellitus with Roux-en-Y gastric bypass
Provedor de dados: 56
Autores: Zhu,Z.; Yin,J.; Li,D.C.; Mao,Z.Q.
The aim of this study was to investigate the effect of Roux-en-Y gastric bypass (RYGB) on the peripheral blood microRNAs (miRNAs) of patients with type 2 diabetes mellitus (T2DM). miRNAs are small 20- to 22-nucleotide (nt) noncoding RNAs. They constitute a novel class of gene regulators that negatively regulate gene expression at the post-transcriptional level. miRNAs play an important role in several biological processes. Twelve patients with T2DM who were scheduled to undergo laparoscopic RYGB surgery were separated into two groups, using a body mass index of 30 kg/m2 as a cut-off point. Venous blood was collected before operation and 12 months after operation. A significant change was observed in the peripheral blood miRNA expression profile of both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Expression profile; Methylation; MicroRNA; Roux-en-Y gastric bypass; Type 2 diabetes mellitus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300705

TRP channels, omega-3 fatty acids, and oxidative stress in neurodegeneration: from the cell membrane to intracellular cross-links
Provedor de dados: 56
Autores: Leonelli,M.; Graciano,M.F.R.; Britto,L.R.G.
The transient receptor potential channels family (TRP channels) is a relatively new group of cation channels that modulate a large range of physiological mechanisms. In the nervous system, the functions of TRP channels have been associated with thermosensation, pain transduction, neurotransmitter release, and redox signaling, among others. However, they have also been extensively correlated with the pathogenesis of several innate and acquired diseases. On the other hand, the omega-3 polyunsaturated fatty acids (n-3 fatty acids) have also been associated with several processes that seem to counterbalance or to contribute to the function of several TRPs. In this short review, we discuss some of the remarkable new findings in this field. We also review the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TRP channels; Calcium; Omega-3 fatty acids; Reactive oxygen species; G-protein coupled receptors; Neurodegeneration.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100003

Late emergence of A594V and L595W mutations related to ganciclovir resistance in a patient with HCMV retinitis and long-term HIV progression
Provedor de dados: 56
Autores: Slavov,S.N.; Vilar,F.C.; Wagatsuma,V.M.D.; Santana,R.C.; Machado,A.A.; Fonseca,B.A.L. da; Kashima,S.; Covas,D.T.
The emergence of ganciclovir (GCV) resistance during the treatment of human cytomegalovirus (HCMV) infection is a serious clinical challenge, and is associated with high morbidity and mortality. In this case report, we describe the emergence of two consecutive mutations (A594V and L595W) related to GCV resistance in a patient with HCMV retinitis and long-term HIV progression after approximately 240 days of GCV use. Following the diagnosis of retinitis, the introduction of GCV did not result in viral load reduction. The detected mutations appeared late in the treatment, and we propose that other factors (high initial HCMV load, previous GCV exposure, low CD4+ cell count), in addition to the presence of resistance mutations, may have contributed to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human cytomegalovirus; HCMV; Ganciclovir; Resistance; AIDS.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900777

Consequences of cerebroventricular insulin injection on renal sodium handling in rats: effect of inhibition of central nitric oxide synthase
Provedor de dados: 56
Autores: Oliveira,P.C.; Michelotto,J.B.; Zapparoli,A.; Boer,P.A.; Gontijo,J.A.R.
In the present study, we investigated the effects of acute intracerebroventricular (icv) insulin administration on central mechanisms regulating urinary sodium excretion in simultaneously centrally NG-nitro-L-arginine methylester (L-NAME)-injected unanesthetized rats. Male Wistar-Hannover rats were randomly assigned to one of five groups: a) icv 0.15 M NaCl-injected rats (control, N = 10), b) icv dose-response (1.26, 12.6 and 126 ng/3 µL) insulin-injected rats (N = 10), c) rats icv injected with 60 µg L-NAME in combination with NaCl (N = 10) or d) with insulin (N = 10), and e) subcutaneously insulin-injected rats (N = 5). Centrally administered insulin produced an increase in urinary output of sodium (NaCl: 855.6 ± 85.1 Δ%/min; 126 ng insulin: 2055 ± 310.6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central nervous system; Intracerebroventricular; Nitric oxide inhibition; Insulin; Natriuresis; Lithium clearance.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200013

Morphological changes induced by testosterone in the mammary glands of female Wistar rats
Provedor de dados: 56
Autores: Chambô-Filho,A.; Camargos,A.F.; Pereira,F.E.L.
Increased levels of androgens in postmenopausal women are considered to be a risk factor for breast cancer. Testosterone, alone or in combination with estrogen, induces epithelial dysplasia and mammary tumors in Noble rats. Since this model of hormone-induced neoplasia has not been reported in other rat strains, we studied the effect of testosterone on the mammary gland morphology of female Wistar rats. Sixty adult, non-castrated, female Wistar rats were implanted in the dorsum midline with a silicone tube containing 50 mg testosterone (testosterone propionate in 30 animals and non-esterified testosterone in the remaining 30 animals) and 20 additional animals were implanted with empty tubes and used as control. Five animals per group were killed 30, 60,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Testosterone; Androgens; Mammary gland; Mammary gland hyperplasia; Mammary gland tumors.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400008

Comparison of endoplasmic reticulum stress and mitochondrial biogenesis responses after 12 weeks of treadmill running and ladder climbing exercises in the cardiac muscle of middle-aged obese rats
Provedor de dados: 56
Autores: Kim,Kijin; Ahn,Nayoung; Jung,Suryun.
The purpose of the present study was to compare the influence of aerobic exercise (AE) lasting 12 weeks to that of resistance exercise (RE) of the same duration on endoplasmic reticulum (ER) stress and mitochondrial biogenesis in the cardiac muscle of middle-aged obese rats. Obesity was induced in thirty 50-week-old male Sprague Dawley rats over 6 weeks by administration of a high-fat diet. The rats were then subjected to treadmill-running (AE) and ladder-climbing (RE) exercises 3 times per week for 12 weeks. Rats in the AE group showed significantly lower increases in body weight and intraperitoneal fat than those in the sedentary control (SC) group (P&lt;0.05). The 12-week exercise regimes resulted in a significant increase in expression of mitochondrial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise training; Mitochondrial biogenesis; Endoplasmic reticulum stress; Obesity; Cardiac muscle; Aging.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000604

Macelignan inhibits bee pathogenic fungi Ascophaera apis growth through HOG1 pathway
Provedor de dados: 56
Autores: Shin,Y.K.; Kim,K.Y.
Ascosphaera apis is a bee pathogen that causes bee larvae infection disease, to which treatment is not yet well investigated. The aim of this study was to investigate antifungal susceptibility in vitro against A. apis and to identify a new antifungal agent for this pathogen through minimal inhibitory concentration (MIC) assay and western blot analysis. Macelignan had 1.56 and 3.125 μg/mL MIC against A. apis after 24 and 48 h, respectively, exhibiting the strongest growth inhibition against A. apis among the tested compounds (corosolic acid, dehydrocostus lactone, loganic acid, tracheloside, fangchinoline and emodin-8-O-β-D-glucopyranoside). Furthermore, macelignan showed a narrow-ranged spectrum against various fungal strains without any mammalian cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bee; Ascosphaera apis; Macelignan; Antifungal agent; HOG1.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700607

Ziyuglycoside II-induced apoptosis in human gastric carcinoma BGC-823 cells by regulating Bax/Bcl-2 expression and activating caspase-3 pathway
Provedor de dados: 56
Autores: Zhu,A.K.; Zhou,H.; Xia,J.Z.; Jin,H.C.; Wang,K.; Yan,J.; Zuo,J.B.; Zhu,X.; Shan,T.
Ziyuglycoside II is an active compound of Sanguisorba officinalis L. that has anti-inflammation, antioxidation, antibiosis, and homeostasis properties. We report here on the anticancer effect of ziyuglycoside II on human gastric carcinoma BGC-823 cells. We investigated the effects of ziyuglycoside II on cell growth, cell cycle, and cell apoptosis of this cell line. Our results revealed that ziyuglycoside II could inhibit the proliferation of BGC-823 cells by inducing apoptosis but not cell cycle arrest, which was associated with regulation of Bax/Bcl-2 expression, and activation of the caspase-3 pathway. Our study is the first to report the antitumor potential of ziyuglycoside II in BGC-823 gastric cancer cells. Ziyuglycoside II may become a potential...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ziyuglycoside II; BGC-823 cells; Apoptosis; Bax/Bcl-2; Caspase-3.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800670

The consensus sequence of FAMLF alternative splice variants is overexpressed in undifferentiated hematopoietic cells
Provedor de dados: 56
Autores: Chen,W.L.; Luo,D.F.; Gao,C.; Ding,Y.; Wang,S.Y.
The familial acute myeloid leukemia related factor gene (FAMLF) was previously identified from a familial AML subtractive cDNA library and shown to undergo alternative splicing. This study used real-time quantitative PCR to investigate the expression of the FAMLF alternative-splicing transcript consensus sequence (FAMLF-CS) in peripheral blood mononuclear cells (PBMCs) from 119 patients with de novo acute leukemia (AL) and 104 healthy controls, as well as in CD34+ cells from 12 AL patients and 10 healthy donors. A 429-bp fragment from a novel splicing variant of FAMLF was obtained, and a 363-bp consensus sequence was targeted to quantify total FAMLF expression. Kruskal-Wallis, Nemenyi, Spearman's correlation, and Mann-Whitney U-tests were used to analyze...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FAMLF; Gene expression; Leukemia; Real-time polymerase chain reaction; Alternative splicing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700603

Rankl expression predicts poor prognosis in gastric cancer patients: results from a retrospective and single-center analysis
Provedor de dados: 56
Autores: Zhang,X.; Song,Y.; Song,N.; Zhang,L.; Wang,Y.; Li,D.; Wang,Z.; Qu,X.; Liu,Y.
The receptor activator of nuclear factor κB ligand (RANKL)/RANK pathway plays an important role in the prognosis of several solid tumor types, but its role in gastric cancer prognosis has been poorly characterized. A total of 116 gastric cancer patients who underwent surgical resection were enrolled in this study. Expressions of RANKL and RANK in gastric cancer tissues were detected using immunohistochemical staining. Thirty-eight patients (33%) showed a high level of RANKL expression and 61 patients (53%) showed a high level of RANK expression. There was a positive correlation between expressions of RANKL and RANK (P=0.014, r=0.221). A high level of RANKL expression indicated shorter overall survival (OS) (P=0.008), and was associated with a higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RANKL; RANK; Gastric cancer; IHC; Overall survival.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300602

In vitro evaluation of the cytotoxic and trypanocidal activities of Ampelozizyphus amazonicus (Rhamnaceae)
Provedor de dados: 56
Autores: Rosas,L.V.; Cordeiro,M.S.C.; Campos,F.R.; Nascimento,S.K.R.; Januário,A.H.; França,S.C.; Nomizo,A.; Toldo,M.P.A.; Albuquerque,S.; Pereira,P.S.
Ampelozizyphus amazonicus Ducke is a tree commonly found in the Amazon region and an extract of its stem bark is popularly used as an antimalarial and anti-inflammatory agent and as an antidote to snake venom. Ursolic acid; five lupane type triterpenes: betulin, betulinic acid, lupenone, 3ß-hydroxylup-20(29)-ene-27,28-dioic acid, and 2a,3ß-dihydroxylup-20(29)-ene-27,28-dioic acid, and three phytosteroids: stigmasterol, sitosterol and campesterol, have been isolated from stem extracts of A. amazonicus Ducke. Their structures were characterized by spectral data including COSY and HMQC. In an in vitro biological screening of the isolated compounds, 3ß-hydroxylup-20(29)-ene-27,28-dioic acid was cytotoxic against the SKBR-3 human adenocarcinoma cell line (1 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ampelozizyphus amazonicus; Rhamnaceae; Pentacyclic triterpenes; Cytotoxic activity; Trypanocidal activity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500009

Diagnosis of Smith-Lemli-Opitz syndrome by ultraviolet spectrophotometry
Provedor de dados: 56
Autores: Scalco,F.B.; Cruzes,V.M.; Vendramini,R.C.; Brunetti,I.L.; Moretti-Ferreira,D.
Smith-Lemli-Opitz syndrome (SLOS) is an autosomal recessive disorder due to an inborn error of cholesterol metabolism, characterized by congenital malformations, dysmorphism of multiple organs, mental retardation and delayed neuropsychomotor development resulting from cholesterol biosynthesis deficiency. A defect in 3ß-hydroxysteroid-delta7-reductase (delta7-sterol-reductase), responsible for the conversion of 7-dehydrocholesterol (7-DHC) to cholesterol, causes an increase in 7-DHC and frequently reduces plasma cholesterol levels. The clinical diagnosis of SLOS cannot always be conclusive because of the remarkable variability of clinical expression of the disorder. Thus, confirmation by the measurement of plasma 7-DHC levels is needed. In the present...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smith-Lemli-Opitz; 3ß-Hydroxysteroid-delta-7-reductase; Cholesterol; 7-Dehydrocholesterol; Ultraviolet spectrophotometry.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000008

Obese obstructive sleep apnea patients with tonsil hypertrophy submitted to tonsillectomy
Provedor de dados: 56
Autores: Martinho,F.L.; Zonato,A.I.; Bittencourt,L.R.A.; Soares,M.C.M.; Silva,R.F.N.; Gregório,L.C.; Tufik,S.
The physiopathology of obstructive sleep apnea-hypopnea syndrome (OSAHS) is multifactorial and obesity has been shown to be one of the main factors correlated with its occurrence. In obese patients with anatomical alterations of the upper airways it is often difficult to predict success for surgical correction since obesity is a limiting factor. Therefore, the aim of the present study was to evaluate the results of tonsillectomy in a specific group of patients, i.e., obese OSAHS patients with tonsil hypertrophy. Seven OSAHS patients with moderate obesity with obstructive palatine tonsil hypertrophy were submitted to tonsillectomy. All patients were submitted to pre- and postoperative appraisal of body mass index, otorhinolaryngology examination and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep apnea syndrome; Obesity; Surgery; Tonsillectomy; Apnea and hypopnea index.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800017

A review of the application of autologous blood transfusion
Provedor de dados: 56
Autores: Zhou,J.
Autologous blood transfusion (ABT) has been gradually attracting more attention due to the increasingly prominent problem of blood transfusion safety and blood shortage in recent years. With the rapid development of blood conservation techniques, blood component separation technology, blood transfusion medicine and a constant increase in clinical needs, ABT technology has been expanded and innovated to a large degree. In this study, the development of preoperative autologous blood donation (PABD), acute normovolemic hemodilution (ANH), intraoperative and postoperative autotransfusion, and other new technologies and theories are reviewed and existing questions are analyzed. Challenges and applications are also discussed in order to provide reference for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autologous blood transfusion; Preoperative autologous blood donation; Acute normovolemic hemodilution; Intraoperative and postoperative autotransfusion.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900301

Respiration, oxidative phosphorylation, and uncoupling protein in Candida albicans
Provedor de dados: 56
Autores: Cavalheiro,R.A.; Fortes,F.; Borecký,J.; Faustinoni,V.C.; Schreiber,A.Z.
The respiration, membrane potential (&lt;FONT FACE=Symbol&gt;Dy&lt;/FONT&gt;), and oxidative phosphorylation of mitochondria in situ were determined in spheroplasts obtained from Candida albicans control strain ATCC 90028 by lyticase treatment. Mitochondria in situ were able to phosphorylate externally added ADP (200 µM) in the presence of 0.05% BSA. Mitochondria in situ generated and sustained stable mitochondrial &lt;FONT FACE=Symbol&gt;Dy&lt;/FONT&gt; respiring on 5 mM NAD-linked substrates, 5 mM succinate, or 100 µM N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride plus 1 mM ascorbate. Rotenone (4 µM) inhibited respiration by 30% and 2 µM antimycin A or myxothiazole and 1 mM cyanide inhibited it by 85%. Cyanide-insensitive respiration was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida albicans spheroplasts; Mitochondria; Respiratory chain; Mitochondrial membrane potential; Uncoupling protein.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000003

Transcriptional up-regulation of PHLDA1 by 17β-estradiol in MCF-7 breast cancer cells
Provedor de dados: 56
Autores: Marchiori,A.C.; Casolari,D.A.; Nagai,M.A.
Most breast cancer risk factors are associated with prolonged exposure of the mammary gland to high levels of estrogens. The actions of estrogens are predominantly mediated by two receptors, ERα and ERβ, which act as transcription factors binding with high affinity to estrogen response elements in the promoter region of target genes. However, most target genes do not contain the consensus estrogen response elements, but rather degenerated palindromic sequences showing one or more mutations and other ER-binding sites such as AP-1 and SP-1. Using the differential display reverse transcription-polymerase chain reaction technique, our group identified several genes differentially expressed in normal tissue and in ER-positive and ER-negative primary breast...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Breast cancer; Gene expression; Estrogen receptor; Estrogen response elements; PHLDA1.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700005

The physiological role of AT1 receptors in the ventrolateral medulla
Provedor de dados: 56
Autores: Tagawa,T.; Fontes,M.A.P.; Potts,P.D.; Allen,A.M.; Dampney,R.A.L.
Neurons in the rostral and caudal parts of the ventrolateral medulla (VLM) play a pivotal role in the regulation of sympathetic vasomotor activity and blood pressure. Studies in several species, including humans, have shown that these regions contain a high density of AT1 receptors specifically associated with neurons that regulate the sympathetic vasomotor outflow, or the secretion of vasopressin from the hypothalamus. It is well established that specific activation of AT1 receptors by application of exogenous angiotensin II in the rostral and caudal VLM excites sympathoexcitatory and sympathoinhibitory neurons, respectively, but the physiological role of these receptors in the normal synaptic regulation of VLM neurons is not known. In this paper we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin receptors; Central cardiovascular pathways; Blood pressure; Hypothalamus; Neurotransmitters.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600005

Comparison of the diagnosis of malaria by microscopy, immunochromatography and PCR in endemic areas of Venezuela
Provedor de dados: 56
Autores: Rodulfo,H; De Donato,M; Mora,R; González,L; Contreras,C.E.
Whole blood samples (N = 295) were obtained from different locations in Amazonas and Sucre States, in Venezuela. Malaria was diagnosed by microscopy, OptiMAL™ and polymerase chain reaction (PCR), with Plasmodium vivax, P. falciparum, and P. malariae being detected when possible. We identified 93 infections, 66 of which were caused by P. vivax, 26 by P. falciparum, and 1 was a mixed infection. No infection caused by P. malariae was detected. The sensitivity and specificity of each diagnostic method were high: 95.7 and 97.9% for microscopy, 87.0 and 97.9% for OptiMAL, and 98.0 and 100% for PCR, respectively. Most samples (72.2%) showed more than 5000 parasites/µL blood. The sensitivity of the diagnosis by microscopy and OptiMAL decreased with lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malaria; Molecular diagnosis of malaria; Microscopic diagnosis of malaria; Polymerase chain reaction; OptiMAL.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400012

Effects of caffeine on the electrophysiological, cognitive and motor responses of the central nervous system
Provedor de dados: 56
Autores: Deslandes,A.C.; Veiga,H.; Cagy,M.; Piedade,R.; Pompeu,F.; Ribeiro,P.
Caffeine is the most consumed psychoactive substance in the world. The effects of caffeine have been studied using cognitive and motor measures, quantitative electroencephalography (qEEG) and event-related potentials. However, these methods are not usually employed in combination, a fact that impairs the interpretation of the results. The objective of the present study was to analyze changes in electrophysiological, cognitive and motor variables with the ingestion of caffeine, and to relate central to peripheral responses. For this purpose we recorded event-related potentials and eyes-closed, resting EEG, applied the Stroop test, and measured reaction time. Fifteen volunteers took caffeine (400 mg) or placebo in a randomized, crossover, double-blind...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quantitative electroencephalography; P300; Caffeine; Cognitive and motor measures; Psychostimulants.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700011

Sida tuberculata (Malvaceae): a study based on development of extractive system and in silico and in vitro properties
Provedor de dados: 56
Autores: da Rosa,H.S.; Salgueiro,A.C.F.; Colpo,A.Z.C.; Paula,F.R.; Mendez,A.S.L.; Folmer,V.
Sida tuberculata (Malvaceae) is a medicinal plant traditionally used in Brazil as an antimicrobial and anti-inflammatory agent. Here, we aimed to investigate the different extractive techniques on phytochemical parameters, as well as to evaluate the toxicity and antioxidant capacity of S. tuberculata extracts using in silico and in vitro models. Therefore, in order to determine the dry residue content and the main compound 20-hydroxyecdysone (20E) concentration, extracts from leaves and roots were prepared testing ethanol and water in different proportions. Extracts were then assessed by Artemia salina lethality test, and toxicity prediction of 20E was estimated. Antioxidant activity was performed by DPPH and ABTS radical scavenger assays, ferric reducing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sida tuberculata; 20-hydroxyecdysone; Toxicity prediction; Antioxidant.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800602

Long-term melatonin treatment reduces ovarian mass and enhances tissue antioxidant defenses during ovulation in the rat
Provedor de dados: 56
Autores: Chuffa,L.G.A.; Amorim,J.P.A.; Teixeira,G.R.; Mendes,L.O.; Fioruci,B.A.; Pinheiro,P.F.F.; Seiva,F.R.F.; Novelli,E.L.B.; Mello Júnior,W.; Martinez,M.; Martinez,F.E.
Melatonin regulates the reproductive cycle, energy metabolism and may also act as a potential antioxidant indoleamine. The present study was undertaken to investigate whether long-term melatonin treatment can induce reproductive alterations and if it can protect ovarian tissue against lipid peroxidation during ovulation. Twenty-four adult female Wistar rats, 60 days old (± 250-260 g), were randomly divided into two equal groups. The control group received 0.3 mL 0.9% NaCl + 0.04 mL 95% ethanol as vehicle, and the melatonin-treated group received vehicle + melatonin (100 µg·100 g body weight-1·day-1) both intraperitoneally daily for 60 days. All animals were killed by decapitation during the morning estrus at 4:00 am. Body weight gain and body mass index...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melatonin; Ovary mass; Lipid peroxidation; Antioxidant defenses; Superoxide dismutase.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300007

Sex differences in angiotensin II- induced hypertension
Provedor de dados: 56
Autores: Xue,B.; Johnson,A.K.; Hay,M.
Sex differences in the development of hypertension and cardiovascular disease have been described in humans and in animal models. In this paper we will review some of our studies which have as their emphasis the examination of the role of sex differences and sex steroids in modulating the central actions of angiotensin II (ANG II) via interactions with free radicals and nitric oxide, generating pathways within brain circumventricular organs and in central sympathomodulatory systems. Our studies indicate that low-dose infusions of ANG II result in hypertension in wild-type male mice but not in intact wild-type females. Furthermore, we have demonstrated that ANG II-induced hypertension in males is blocked by central infusions of the androgen receptor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; Estrogen; Estrogen a and ß receptors; Nitric oxide; Reactive oxygen species; Testosterone.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500018

Developed pressure data may provide misinformation when used alone to evaluate systolic function in isovolumetric left ventricle preparations
Provedor de dados: 56
Autores: Murad,N.; Tucci,P.J.F.
We report data showing that developed pressure (DPmax) may lead to opposite conclusion with respect to maximal developed circumferential wall stress (&lt;FONT FACE="Symbol"&gt;s&lt;/FONT&gt;max) when used to assess contractile function in left ventricle isovolumic preparations. Isovolumetric left ventricle preparations of rats with cardiac hypertrophy (H; N = 10) induced by isoproterenol administration showed higher DPmax (174 ± 14 mmHg) than control (C; N = 8) animals (155 ± 12 mmHg) or rats with regression (R; N = 8) of hypertrophy (144 ± 11 mmHg). In contrast, the estimated &lt;FONT FACE="Symbol"&gt;s&lt;/FONT&gt;max for C (145 ± 26 kdynes/cm2) and R (133 ± 17 kdynes/cm2) was higher than for H (110 ± 13 kdynes/cm2). According to Laplace's law, the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Contractile function; Developed pressure; Wall stress; Cardiac hypertrophy; Laplace's law.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600014

Coronary artery plaque burden and calcium scores in healthy men adhering to long-term wine drinking or alcohol abstinence
Provedor de dados: 56
Autores: da Luz,P.L.; Coimbra,S.; Favarato,D.; Albuquerque,C.; Mochiduky,R.I.; Rochitte,C.E.; Hojaij,E.; Gonsalves,C.R.L.; Laurindo,F.R.
Observational studies suggest there are clinical benefits to moderate red wine (RW) consumption. However, the effects on coronary vasculature and overall lifestyle are unclear. We investigated whether a lifestyle of regular long-term RW consumption is associated with changes in coronary plaque burden, calcium score, carotid intima/media thickness, endothelial function, and metabolic variables, compared with alcohol abstinence. Healthy volunteers were evaluated by coronary computed tomography angiography (CTA) as well as carotid and brachial artery ultrasound. Nutritional status, psychological status, and metabolic variables were assessed. The study included 101 drinkers [aged 58.9±7.3 years (means±SD)], from wine brotherhoods, and 104 abstainers, from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Red wine; Lifestyle; Computer tomography angiography; Coronary calcium; Carotid plaques.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800697

Do socioeconomic factors explain why maternal smoking during pregnancy is more frequent in a more developed city of Brazil?
Provedor de dados: 56
Autores: Ribeiro,V.S.; Figueiredo,F.P.; Silva,A.A.M.; Batista,R.L.F.; Barbieri,M.A.; Lamy Filho,F.; Alves,M.T.S.S.B.; Santos,A.M.; Bettiol,H.
The prevalence of smoking during pregnancy in Ribeirão Preto, a rich Brazilian city, was significantly higher (21.4%) than in São Luís (5.9%), a less developed city. To assess which variables explain the difference in prevalence of smoking during pregnancy, data from two birth cohorts were used, including 2846 puerperae from Ribeirão Preto, in 1994, and 2443 puerperae from São Luís, in 1997/98. In multivariable analysis, risk of maternal smoking during pregnancy was higher in São Luís for mothers living in a household with five or more persons (OR = 1.72, 95%CI = 1.12-2.64), aged 35 years or older (OR = 1.98, 95%CI = 0.99-3.96), who had five or more children (OR = 2.10, 95%CI = 1.16-3.81), and whose companion smoked (OR = 2.20, 95%CI = 1.52-3.18). Age of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smoking; Pregnancy; Risk factors; Perinatal complications; Placenta previa.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900007

Effect of vitamin D therapy in addition to amitriptyline on migraine attacks in pediatric patients
Provedor de dados: 56
Autores: Cayir,A.; Turan,M.I.; Tan,H.
The purpose of this study was to investigate the effect of supplementary vitamin D therapy in addition to amitriptyline on the frequency of migraine attacks in pediatric migraine patients. Fifty-three children 8-16 years of age and diagnosed with migraine following the International Headache Society 2005 definition, which includes childhood criteria, were enrolled. Patients were classified into four groups on the basis of their 25-hydroxyvitamin D [25(OH)D] levels. Group 1 had normal 25(OH)D levels and received amitriptyline therapy alone; group 2 had normal 25(OH)D levels and received vitamin D supplementation (400 IU/day) plus amitriptyline; group 3 had mildly deficient 25(OH)D levels and received amitriptyline plus vitamin D (800 IU/day); and group 4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Children; Migraine; Vitamin D.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400349

Effect of conditioned medium of mesenchymal stem cells on the in vitro maturation and subsequent development of mouse oocyte
Provedor de dados: 56
Autores: Ling,B.; Feng,D.Q.; Zhou,Y.; Gao,T.; Wei,H.M.; Tian,Z.G.
Mesenchymal stem cells (MSCs) secrete a variety of cytokines and growth factors in addition to self-renewal and multiple forms of differentiation. Some of these secreted bioactive factors could improve meiotic maturation in vitro and subsequent embryo developmental potential. The aim of the present study was to determine whether in vitro maturation (IVM) of mouse oocyte with or without cumulus cells could be improved by contact with conditioned medium (CM) of MSCs as well as the efficiency of CM to support follicular growth and oocyte maturation in the ovarian organ of mice cultured on soft agar. The developmental potential of matured oocyte was assessed by blastocyst formation after in vitro fertilization (IVF). Germinal vesicle stage oocytes with or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem cells; In vitro maturation; Follicular development; Mouse oocyte development.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100006

Viral membrane fusion: is glycoprotein G of rhabdoviruses a representative of a new class of viral fusion proteins?
Provedor de dados: 56
Autores: Da Poian,A.T.; Carneiro,F.A.; Stauffer,F.
Enveloped viruses always gain entry into the cytoplasm by fusion of their lipid envelope with a cell membrane. Some enveloped viruses fuse directly with the host cell plasma membrane after virus binding to the cell receptor. Other enveloped viruses enter the cells by the endocytic pathway, and fusion depends on the acidification of the endosomal compartment. In both cases, virus-induced membrane fusion is triggered by conformational changes in viral envelope glycoproteins. Two different classes of viral fusion proteins have been described on the basis of their molecular architecture. Several structural data permitted the elucidation of the mechanisms of membrane fusion mediated by class I and class II fusion proteins. In this article, we review a number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Membrane fusion; Fusion proteins; Rhabdovirus; G protein; Glycoprotein.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600002

Antioxidant and antimicrobial activities of Bauhinia racemosa L. stem bark
Provedor de dados: 56
Autores: Kumar,R.S.; Sivakumar,T.; Sunderam,R.S.; Gupta,M.; Mazumdar,U.K.; Gomathi,P.; Rajeshwar,Y.; Saravanan,S.; Kumar,M.S.; Murugesh,K.; Kumar,K.A.
The present study was carried out to evaluate the antioxidant and antimicrobial activities of a methanol extract of Bauhinia racemosa (MEBR) (Caesalpiniaceae) stem bark in various systems. 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical, superoxide anion radical, nitric oxide radical, and hydroxyl radical scavenging assays were carried out to evaluate the antioxidant potential of the extract. The antioxidant activity of the methanol extract increased in a concentration-dependent manner. About 50, 100, 250, and 500 µg MEBR inhibited the peroxidation of a linoleic acid emulsion by 62.43, 67.21, 71.04, and 76.83%, respectively. Similarly, the effect of MEBR on reducing power increased in a concentration-dependent manner. In DPPH radical scavenging assays the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bauhinia racemosa; Antioxidant activity; Lipid peroxidation; Scavenging superoxide; DPPH assay; Antimicrobial activity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700004

Risk factors for alloimmunization by patients with sickle cell disease
Provedor de dados: 56
Autores: Murao,M.; Viana,M.B.
Blood transfusion in patients with sickle cell disease (SCD) is limited by the development of alloantibodies to erythrocytes. In the present study, the frequency and risk factors for alloimmunization were determined. Transfusion records and medical charts of 828 SCD patients who had been transfused and followed at the Belo Horizonte Blood Center, Belo Horizonte, MG, Brazil, were retrospectively reviewed. Alloimmunization frequency was 9.9% (95% CI: 7.9 to 11.9%) and 125 alloantibodies were detected, 79% of which belonged to the Rhesus and Kell systems. Female patients developed alloimmunization more frequently (P = 0.03). The median age of the alloimmunized group was 23.3 years, compared to 14.6 years for the non-alloimmunized group (P &lt; 0.0001)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sickle cell disease; Red cell alloimmunization; Blood cell transfusion; Transfusion reactions.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500004

Development and validation of a genotype 3 recombinant protein-based immunoassay for hepatitis E virus serology in swine
Provedor de dados: 56
Autores: van der Poel,W.H.M.; Pavio,N.; van der Goot,J.; van Es,M.; Martin,M.; Engel,B.
Hepatitis E virus (HEV) is classified within the family Hepeviridae, genus Hepevirus. HEV genotype 3 (Gt3) infections are endemic in pigs in Western Europe and in North and South America and cause zoonotic infections in humans. Several serological assays to detect HEV antibodies in pigs have been developed, at first mainly based on HEV genotype 1 (Gt1) antigens. To develop a sensitive HEV Gt3 ELISA, a recombinant baculovirus expression product of HEV Gt3 open reading frame-2 was produced and coated onto polystyrene ELISA plates. After incubation of porcine sera, bound HEV antibodies were detected with anti-porcine anti-IgG and anti-IgM conjugates. For primary estimation of sensitivity and specificity of the assay, sets of sera were used from pigs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis E virus; Zoonosis; Swine; ELISA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400334

Arsenic trioxide reduces the invasive and metastatic properties of nasopharyngeal carcinoma cells in vitro
Provedor de dados: 56
Autores: Du,C.W.; Wen,B.G.; Li,D.R.; Peng,X.; Hong,C.Q.; Chen,J.Y.; Lin,Z.Z.; Hong,X.; Lin,Y.C.; Xie,L.X.; Wu,M.Y.; Zhang,H.
Nasopharyngeal carcinoma (NPC) is notorious for the metastases, which are in close association with Epstein-Barr virus-encoded latent membrane protein 1 (LMP1). Arsenic trioxide (As2O3) has been shown to induce apoptosis and differentiation in NPC xenografts. Then, can it repress the cancer cells' metastasis potential? To elucidate this issue, the present study was performed. LMP1-negative cell line HNE1 and LMP1-positive cell line HNE1-LMP1 were used as in vitro model. Cells (1 x 10(5)/mL) were cultured with or without 3 µM As2O3 for 48 h. Then the survival cells were collected to investigate their potential of colony formation, attachment, invasion, and migration. Both confocal immunofluorescence staining and Western blot were used to detect the changes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arsenic trioxide (As2O3); Nasopharyngeal carcinoma; Metastases; Latent membrane protein 1; Metalloproteinase 9.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500015

Quenching of the intrinsic fluorescence of bovine serum albumin by chlorpromazine and hemin
Provedor de dados: 56
Autores: Silva,D.; Cortez,C.M.; Louro,S.R.W.
The binding of chlorpromazine (CPZ) and hemin to bovine serum albumin was studied by the fluorescence quenching technique. CPZ is a widely used anti-psychotic drug that interacts with blood components, influences bioavailability, and affects function of several biomolecules. Hemin is an important ferric residue of hemoglobin that binds within the hydrophobic region of albumin with high specificity. Quenching of the intrinsic fluorescence of bovine serum albumin (BSA) was observed by selectively exciting tryptophan residues at 290 nm. Emission spectra were recorded in the range from 300 to 450 nm for each quencher addition. Stern-Volmer graphs were plotted, and the quenching constant estimated for BSA solution titrated with hemin at 25ºC was 1.44 (± 0.05) x...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chlorpromazine; Hemin; Albumin; Fluorescence quenching.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700004

Baicalin reduces the severity of experimental autoimmune encephalomyelitis
Provedor de dados: 56
Autores: Zeng,Y.; Song,C.; Ding,X.; Ji,X.; Yi,L.; Zhu,K.
Scutellaria baicalensis Georgi is one of the important medicinal herbs widely used for the treatment of various inflammatory diseases in Asia. Baicalin (BA) is a bioactive anti-inflammatory flavone found abundantly in Scutellaria baicalensis Georgi. To explore the therapeutic potential of BA, we examined the effects of systemic administration of the flavone (5 and 10 mg/kg, ip) on relapsing/remitting experimental autoimmune encephalomyelitis (EAE) induced by proteolipid protein 139-151 in SJL/J mice, an experimental model of multiple sclerosis. The mice treated with PBS or BA at day -1 and for 3 consecutive days were observed daily for clinical signs of disease up to 60 days after immunization. In the PBS-EAE group, neurological scores were: incidence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental autoimmune encephalomyelitis; Baicalin; IFN-g; IL-4; Neurologic score.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700016

Age-related changes and effects of regular low-intensity exercise on gait, balance, and oxidative biomarkers in the spinal cord of Wistar rats
Provedor de dados: 56
Autores: Silveira,E.M.S.; Kroth,A.; Santos,M.C.Q.; Silva,T.C.B.; Silveira,D.; Riffel,A.P.K.; Scheid,T.; Trapp,M.; Partata,W.A.
The present study aimed to analyze age-related changes to motor coordination, balance, spinal cord oxidative biomarkers in 3-, 6-, 18-, 24-, and 30-month-old rats. The effects of low-intensity exercise on these parameters were also analyzed in 6-, 18-, and 24-month-old rats. Body weight, blood glucose, total cholesterol, and high-density lipoprotein (HDL) cholesterol were assessed for all rats. The soleus muscle weight/body weight ratio was used to estimate skeletal muscle mass loss. Body weight increased until 24 months; only 30-month-old rats exhibited decreased blood glucose and increased total cholesterol and HDL cholesterol. The soleus muscle weight/body weight ratio increased until 18 months, followed by a small decrease in old rats. Exercise did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superoxide anion generation; Hydrogen peroxide; Total thiol content; Lipid hydroperoxide levels; Total antioxidant capacity; Superoxide dismutase.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700608

Gap junctions in the cardiovascular and immune systems
Provedor de dados: 56
Autores: Rozental,R.; Campos-de-Carvalho,A.C.; Spray,D.C.
Gap junctions are clusters of intercellular channels directly connecting the cytoplasm of adjacent cells. These channels are formed by proteins named connexins and are present in all metazoan organisms where they serve diverse functions ranging from control of cell growth and differentiation to electric conduction in excitable tissues. In this overview we describe the presence of connexins in the cardiovascular and lympho-hematopoietic systems giving the reader a summary of the topics to be covered throughout this edition and a historical perspective of the discovery of gap junctions in the immune system.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gap junctions; Cardiovascular system; Immune system; Connexins.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400001

A new brain metalloendopeptidase which degrades the Alzheimer ß-amyloid 1-40 peptide producing soluble fragments without neurotoxic effects
Provedor de dados: 56
Autores: Carvalho,K.M.; França,M.S.F.; Camarão,G.C.; Ruchon,A.F.
A new metalloendopeptidase was purified to apparent homogeneity from a homogenate of normal human brain using successive steps of chromatography on DEAE-Trisacryl, hydroxylapatite and Sephacryl S-200. The purified enzyme cleaved the Gly33-Leu34 bond of the 25-35 neurotoxic sequence of the Alzheimer ß-amyloid 1-40 peptide producing soluble fragments without neurotoxic effects. This enzyme activity was only inhibited by divalent cation chelators such as EDTA, EGTA and o-phenanthroline (1 mM) and was insensitive to phosphoramidon and captopril (1 µM concentration), specific inhibitors of neutral endopeptidase (EC 3.4.24.11) and angiotensin-converting enzyme (EC 3.4.15.1), respectively. The high affinity of this human brain endopeptidase for ß-amyloid 1-40...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain metalloendopeptidase; Alzheimer ß-amyloid 1-40 peptide; Alzheimer's disease.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000002

Diagnostic and prognostic value of procalcitonin for early intracranial infection after craniotomy
Provedor de dados: 56
Autores: Yu,Y.; Li,H.J.
Intracranial infection is a common clinical complication after craniotomy. We aimed to explore the diagnostic and prognostic value of dynamic changing procalcitonin (PCT) in early intracranial infection after craniotomy. A prospective study was performed on 93 patients suspected of intracranial infection after craniotomy. Routine peripheral venous blood was collected on the day of admission, and C reactive protein (CRP) and PCT levels were measured. Cerebrospinal fluid (CSF) was collected for routine biochemical, PCT and culture assessment. Serum and CSF analysis continued on days 1, 2, 3, 5, 7, 9, and 11. The patients were divided into intracranial infection group and non-intracranial infection group; intracranial infection group was further divided into...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Intracranial infection; Procalcitonin; Cerebrospinal fluid procalcitonin; Craniotomy; Diagnosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500608

A randomized crossover clinical study showing that methylphenidate-SODAS improves attention-deficit/hyperactivity disorder symptoms in adolescents with substance use disorder
Provedor de dados: 56
Autores: Szobot,C.M.; Rohde,L.A.; Katz,B.; Ruaro,P.; Schaefer,T.; Walcher,M.; Bukstein,O.; Pechansky,F.
Our objective was to evaluate the effectiveness of a long-acting formulation of methylphenidate (MPH-SODAS) on attention-deficit/hyperactivity disorder (ADHD) symptoms in an outpatient sample of adolescents with ADHD and substance use disorders (SUD). Secondary goals were to evaluate the tolerability and impact on drug use of MPH-SODAS. This was a 6-week, single-blind, placebo-controlled crossover study assessing efficacy of escalated doses of MPH-SODAS on ADHD symptoms in 16 adolescents with ADHD/SUD. Participants were randomly allocated to either group A (weeks 1-3 on MPH-SODAS, weeks 4-6 on placebo) or group B (reverse order). The primary outcome measures were the Swanson, Nolan and Pelham Scale, version IV (SNAP-IV) and the Clinical Global Impression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attention deficit/hyperactivity disorder; Methylphenidate; Substance use disorder; Treatment; Adolescents.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300012

An ultrasound and histomorphological analysis of experimental liver cirrhosis in rats
Provedor de dados: 56
Autores: Dias,J.V.; Paredes,B.D.; Mesquita,L.F.Q.; Carvalho,A.B.; Kozlowski,E.O.; Lessa,A.S.; Takiya,C.M.; Resende,C.M.C.; Coelho,H.S.M.; Campos-de-Carvalho,A.C.; Rezende,G.F.M.; Goldenberg,R.C.S.
We investigated whether liver injury by dual exposure to ethanol and carbon tetrachloride (EtOH + CCl4) for 15 weeks would persist after hepatotoxic agents were removed (EtOH + CCl4/8wR). After 15 weeks of hepatic injury with ethanol (5.5%, m/v) and carbon tetrachloride (0.05, mL/kg, ip), 5 of 11 female Wistar rats were sacrificed. The other 6 rats were maintained for an additional 8 weeks without hepatotoxic agents. Ultrasonography showed increased liver echogenicity and dilation of portal vein caliber in both groups (EtOH + CCl4: 0.22 ± 0.01 cm, P &lt; 0.001; EtOH + CCl4/8wR: 0.21 ± 0.02 cm, P &lt; 0.01) vs control (0.16 ± 0.02 cm). Histopathology showed regenerative nodules in both experimental groups. Histomorphometry revealed increased fibrosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat; Cirrhosis; Collagen; Ultrasound; CCl4; Ethanol.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100008

Effects of systemic administration of ciliary neurotrophic factor on Bax and Bcl-2 proteins in the lumbar spinal cord of neonatal rats after sciatic nerve transection
Provedor de dados: 56
Autores: Rezende,A.C.S.; Vieira,A.S.; Rogério,F.; Rezende,L.F.; Boschero,A.C.; Negro,A.; Langone,F.
Ciliary neurotrophic factor (CNTF) is a cytokine that plays a neuroprotective role in relation to axotomized motoneurons. We determined the effect of daily subcutaneous doses of CNTF (1.2 µg/g for 5 days; N = 13) or PBS (N = 13) on the levels of mRNA for Bcl-2 and Bax, as well as the expression and inter-association of Bcl-2 and Bax proteins, and the survival of motoneurons in the spinal cord lumbar enlargement of 2-day-old Wistar rats after sciatic nerve transection. Five days after transection, the effects were evaluated on histological and molecular levels using Nissl staining, immunoprecipitation, Western blot analysis, and reverse transcriptase-polymerase chain reaction. The motoneuron survival ratio, defined as the ratio between the number of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ciliary neurotrophic factor; Bax; Neonatal rats; Axotomy; Motoneuron; Immunoprecipitation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100013

Voluntary exercise prior to traumatic brain injury alters miRNA expression in the injured mouse cerebral cortex
Provedor de dados: 56
Autores: Miao,W.; Bao,T.H.; Han,J.H.; Yin,M.; Yan,Y.; Wang,W.W.; Zhu,Y.H.
MicroRNAs (miRNAs) may be important mediators of the profound molecular and cellular changes that occur after traumatic brain injury (TBI). However, the changes and possible roles of miRNAs induced by voluntary exercise prior to TBI are still not known. In this report, the microarray method was used to demonstrate alterations in miRNA expression levels in the cerebral cortex of TBI mice that were pretrained on a running wheel (RW). Voluntary RW exercise prior to TBI: i) significantly decreased the mortality rate and improved the recovery of the righting reflex in TBI mice, and ii) differentially changed the levels of several miRNAs, upregulating some and downregulating others. Furthermore, we revealed global upregulation of miR-21, miR-92a, and miR-874 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Voluntary exercise; Running wheel; MiRNA; Traumatic brain injury.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500433

A single-step purification of bothropstoxin-1
Provedor de dados: 56
Autores: Spencer,P.J.; Aird,S.D.; Boni-Mitake,M.; Nascimento,N.; Rogero,J.R.
Bothrops venoms are complex mixtures of components with a wide range of biological activities. Among these substances, myotoxins have been investigated by several groups. Bothropstoxin-1 (Bthtx-1) is a phospholipase A2-like basic myotoxin from Bothrops jararacussu. The purification of this component involves two chromatographic steps. Although providing a pure material, the association of these two steps is time consuming and a single-step method using high performance chromatography media would be useful. In the present study, we describe a single-step purification method for Bthtx-1. Bothrops jararacussu venom was dissolved in 1 ml buffer. After centrifugation, the supernatant was injected into a Resource-S cation exchange column connected to an FPLC...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myotoxins; Purification; Chromatography; Bothrops jararacussu; Bothropstoxin-1.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900004

Effect of the consumption of tomato paste on plasma prostate-specific antigen levels in patients with benign prostate hyperplasia
Provedor de dados: 56
Autores: Edinger,M.S.; Koff,W.J.
The consumption of tomatoes and tomato products has been associated with a reduced risk of prostate cancer. We observed a decrease of 10.77% in prostate-specific antigen (PSA) levels in patients with benign prostate hyperplasia who were submitted to daily ingestion of tomato paste. This was an experimental rather than a controlled study with a sample of 43 men ranging in age from 45 to 75 years, all with histological diagnoses of benign prostate hyperplasia and plasma PSA levels of 4-10 ng/mL. All patients received 50 g of tomato paste once a day for 10 consecutive weeks and PSA levels were analyzed before, during and after the consumption of tomato paste. ANOVA for repeated measures was used to compare PSA levels before, during and after the consumption...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Prostate cancer; Prostate-specific antigen; Tomatoes; Tomato paste; Lycopene.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800014

Role of the cardiac nerve in the adaptive changes of heart rate in response to an aversive stimulus in Megalobulimus mogianensis
Provedor de dados: 56
Autores: Romero,S.M.B.; Hoffmann,A.
The effect of an aversive stimulus represented by contact with a hot plate on the heart rate of Megalobulimus mogianensis was evaluated with electrocardiogram recording in intact snails (N = 8). All stimulated animals showed an increase in heart rate, with mean values ranging from 35.6 ± 1.2 (basal heart rate) to 43.8 ± 0.9 bpm (post-stimulation heart rate). The cardioacceleration was followed by gradual recovery of the basal heart rate, with mean recovery times varying from 4.3 ± 0.3 to 5.8 ± 0.6 min. Repetition of the stimulus did not affect the magnitude of variation nor did it influence the basal heart rate recovery time. To investigate the role of the cardiac nerve in mediating the heart rate alterations induced by the aversive stimulus, denervated (N...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Aversive stimulus; Cardiac nerve; Heart rate; Snail; Megalobulimus mogianensis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500013

The use of genes for performance enhancement: doping or therapy?
Provedor de dados: 56
Autores: Oliveira,R.S.; Collares,T.F.; Smith,K.R.; Collares,T.V.; Seixas,F.K.
Recent biotechnological advances have permitted the manipulation of genetic sequences to treat several diseases in a process called gene therapy. However, the advance of gene therapy has opened the door to the possibility of using genetic manipulation (GM) to enhance athletic performance. In such ‘gene doping’, exogenous genetic sequences are inserted into a specific tissue, altering cellular gene activity or leading to the expression of a protein product. The exogenous genes most likely to be utilized for gene doping include erythropoietin (EPO), vascular endothelial growth factor (VEGF), insulin-like growth factor type 1 (IGF-1), myostatin antagonists, and endorphin. However, many other genes could also be used, such as those involved in glucose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic manipulation; Erythropoietin; Vascular endothelial growth factor; Insulin-like growth factor-1; Myostatin; Bioethics.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200001

Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells
Provedor de dados: 56
Autores: Lauand,C.; Niero,E.L.; Dias,V.M.; Machado-Santelli,G.M.
Lung cancer often exhibits molecular changes, such as the overexpression of the ErbB1 gene that encodes epidermal growth factor receptor (EGFR). ErbB1 amplification and mutation are associated with tumor aggressiveness and low response to therapy. The aim of the present study was to design a schedule to synchronize the cell cycle of A549 cell line (a non-small cell lung cancer) and to analyze the possible association between the micronuclei (MNs) and the extrusion of ErbB1 gene extra-copies. After double blocking, by the process of fetal bovine serum deprivation and vincristine treatment, MNs formation was monitored with 5-bromo-2-deoxyuridine (BrdU) incorporation, which is an S-phase marker. Statistical analyses allowed us to infer that MNs may arise both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: A549 cells; Cell cycle synchronization; BrdU incorporation; ErbB1; Micronucleus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500382

Insights into the physiological function of cellular prion protein
Provedor de dados: 56
Autores: Martins,V.R.; Mercadante,A.F.; Cabral,A.L.B.; Freitas,A.R.O.; Castro,R.M.R.P.S.
Prions have been extensively studied since they represent a new class of infectious agents in which a protein, PrPsc (prion scrapie), appears to be the sole component of the infectious particle. They are responsible for transmissible spongiform encephalopathies, which affect both humans and animals. The mechanism of disease propagation is well understood and involves the interaction of PrPsc with its cellular isoform (PrPc) and subsequently abnormal structural conversion of the latter. PrPc is a glycoprotein anchored on the cell surface by a glycosylphosphatidylinositol moiety and expressed in most cell types but mainly in neurons. Prion diseases have been associated with the accumulation of the abnormally folded protein and its neurotoxic effects;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PrPc; Cellular function; Transmissible spongiform encephalopathies; Laminin; Signal transduction; Copper.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500005

Are high-lysine cereal crops still a challenge?
Provedor de dados: 56
Autores: Ferreira,R.R.; Varisi,V.A.; Meinhardt,L.W.; Lea,P.J.; Azevedo,R.A.
The essential amino acids lysine and threonine are synthesized in higher plants via a pathway starting with aspartate that also leads to the formation of methionine and isoleucine. Lysine is one of most limiting amino acids in plants consumed by humans and livestock. Recent genetic, molecular, and biochemical evidence suggests that lysine synthesis and catabolism are regulated by complex mechanisms. Early kinetic studies utilizing mutants and transgenic plants that over-accumulate lysine have indicated that the major step for the regulation of lysine biosynthesis is at the enzyme dihydrodipicolinate synthase. Despite this tight regulation, recent strong evidence indicates that lysine catabolism is also subject to control, particularly in cereal seeds. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lysine; Threonine; Aspartate; Nutritionally improved crops; Methionine; Amino acids.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700002

Compound 48/80, a histamine-depleting agent, blocks the protective effect of morphine against electroconvulsive shock in mice
Provedor de dados: 56
Autores: Karadag,C.H.; Dokmeci,D.; Dost,T.; Ulugol,A.; Dokmeci,I.
We have shown that morphine has an anticonvulsive effect against maximal electroconvulsive shock (MES) in mice, and this effect is antagonized by histamine H1-receptor antagonists. Brain histamine is localized both in neurons and in mast cells, and morphine is known to enhance the turnover of neuronal histamine and to release histamine from mast cells. In the present experiments, compound 48/80 was injected chronically (0.5 mg/kg on day 1, 1 mg/kg on day 2, 2 mg/kg on day 3, 3 mg/kg on day 4, and 4 mg/kg on day 5, twice daily, ip) to deplete mast cell contents. Morphine (0.001-10 mg/kg, ip; N = 20) produced a dose-dependent anticonvulsive effect against MES seizure in mice with non-depleted mast cells, whereas it did not exert any anticonvulsive effect in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphine; Anticonvulsive effect; Histamine; Mast cells; Compound 48/80; Electroshock.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300011

Familial hyperaldosteronism
Provedor de dados: 56
Autores: Torpy,D.J.; Stratakis,C.A.; Chrousos,G.P.
Aldosterone, the major circulating mineralocorticoid, participates in blood volume and serum potassium homeostasis. Primary aldosteronism is a disorder characterised by hypertension and hypokalaemia due to autonomous aldosterone secretion from the adrenocortical zona glomerulosa. Improved screening techniques, particularly application of the plasma aldosterone:plasma renin activity ratio, have led to a suggestion that primary aldosteronism may be more common than previously appreciated among adults with hypertension. Glucocorticoid-remediable aldosteronism (GRA) was the first described familial form of hyperaldosteronism. The disorder is characterised by aldosterone secretory function regulated chronically by ACTH. Hence, aldosterone hypersecretion can be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aldosterone; Hyperaldosteronism; Genetics; Adrenal tumour; Hypertension.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000004

One hundred million years of interhemispheric communication: the history of the corpus callosum
Provedor de dados: 56
Autores: Aboitiz,F.; Montiel,J.
Analysis of regional corpus callosum fiber composition reveals that callosal regions connecting primary and secondary sensory areas tend to have higher proportions of coarse-diameter, highly myelinated fibers than callosal regions connecting so-called higher-order areas. This suggests that in primary/secondary sensory areas there are strong timing constraints for interhemispheric communication, which may be related to the process of midline fusion of the two sensory hemifields across the hemispheres. We postulate that the evolutionary origin of the corpus callosum in placental mammals is related to the mechanism of midline fusion in the sensory cortices, which only in mammals receive a topographically organized representation of the sensory surfaces. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Commissures; Evolution; Interhemispheric transfer; Lateralization; Synchronization.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400002

Cardiac function and intracellular Ca2+ handling proteins are not impaired by high-saturated-fat diet-induced obesity
Provedor de dados: 56
Autores: Deus,A.F.; Vileigas,D.F.; Silva,D.C.T.; Tomasi,L.C.; Campos,D.H.S.; Okoshi,K.; Padovani,C.R.; Cicogna,A.C.
Obesity is often associated with changes in cardiac function; however, the mechanisms responsible for functional abnormalities have not yet been fully clarified. Considering the lack of information regarding high-saturated-fat diet-induced obesity, heart function, and the proteins involved in myocardial calcium (Ca2+) handling, the aim of this study was to test the hypothesis that this dietary model of obesity leads to cardiac dysfunction resulting from alterations in the regulatory proteins of intracellular Ca2+ homeostasis. Male Wistar rats were distributed into two groups: control (C, n=18; standard diet) and obese (Ob, n=19; high-saturated-fat diet), which were fed for 33 weeks. Cardiac structure and function were evaluated using echocardiographic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Saturated fatty acids; High-fat diet; Cardiac function; Calcium handling proteins; Rat.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600605

Serum microRNA-30c levels are correlated with disease progression in Xinjiang Uygur patients with chronic hepatitis B
Provedor de dados: 56
Autores: Zhang,J.; Ma,J.; Wang,H.; Guo,L.; Li,J.
We aimed to investigate the potential role and mechanism of microRNA-30c (miR-30c) in the pathological development of chronic hepatitis B (CHB). The serum levels of miR-30c in hepatitis B virus (HBV) carrier Xinjiang Uygur patients with inactive, low-replicative, high-replicative and HBe antigen-positive CHB were investigated. HepG2 cells were co-transfected with pHBV1.3 and miR-30c mimic or inhibitor or scramble RNA. The effects of miR-30c dysregulation on HBV replication and gene expression, cell proliferation and cell cycle were then investigated. miR-30c was down-regulated in Xinjiang Uygur patients with CHB compared to healthy controls and its expression level discriminated HBV carrier patients with inactive, low-replicative, high-replicative and HBe...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic hepatitis B; Disease progression; MicroRNA-30c; Hepatitis B virus replication; Cell proliferation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600601

Pharmacological characterization of the relaxant effect induced by adrenomedullin in rat cavernosal smooth muscle
Provedor de dados: 56
Autores: Leite,L.N.; Gonzaga,N.A.; Tirapelli,D.P.C.; Tirapelli,L.F.; Tirapelli,C.R.
The aim of the present study was to determine the mechanisms underlying the relaxant effect of adrenomedullin (AM) in rat cavernosal smooth muscle (CSM) and the expression of AM system components in this tissue. Functional assays using standard muscle bath procedures were performed in CSM isolated from male Wistar rats. Protein and mRNA levels of pre-pro-AM, calcitonin receptor-like receptor (CRLR), and Subtypes 1, 2 and 3 of the receptor activity-modifying protein (RAMP) family were assessed by Western immunoblotting and quantitative real-time polymerase chain reaction, respectively. Nitrate and 6-keto-prostaglandin F1α (6-keto-PGF1α; a stable product of prostacyclin) levels were determined using commercially available kits. Protein and mRNA of AM, CRLR,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat cavernosal smooth muscle; Adrenomedullin; Relaxation; Nitric oxide; Calcitonin receptor-like receptor.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000876

Antibodies to citrullinated peptides are not associated with the rate of joint destruction in patients with a well-established diagnosis of rheumatoid arthritis
Provedor de dados: 56
Autores: Nieto-Colonia,A.M.; Santos,W.S.; Keusseyan,S.P.; Caldana,W.; Fernandes,A.R.C.; Andrade,L.E.C.
Antibodies to citrullinated peptides are highly specific for rheumatoid arthritis (RA) and represent a significant risk factor for undifferentiated polyarthritis. This prognostic ability may be related to the very diagnostic performance of these autoantibodies, since RA is a more erosive disease than other forms of arthritis. The present study evaluated an association of antibodies to citrullinated peptides and the rate of joint destruction in patients with a well-established diagnosis of RA. Seventy-one patients with RA were evaluated in 1994 and again in 2002 (functional class, joint count, Health Assessment Questionnaire score, hands X-ray). Autoantibodies (rheumatoid factor (RF), anti-perinuclear factor, anti-cyclic citrullinated peptide (CCP)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheumatoid arthritis; Rheumatoid factor; Autoantibodies; Citrullinated peptides; Disease severity; Joint imaging.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300002

The 5alpha-reductase type 1, but not type 2, gene is expressed in anagen hairs plucked from the vertex area of the scalp of hirsute women and normal individuals
Provedor de dados: 56
Autores: Oliveira,I.O.; Lhullier,C.; Brum,I.S.; Spritzer,P.M.
The aim of the present study was to determine the expression of the genes for type 1 (SDR5A1) and type 2 (SDR5A2) 5alpha-reductase isoenzymes in scalp hairs plucked from 33 hirsute patients (20 with polycystic ovary syndrome and 13 with idiopathic hirsutism) and compare it with that of 10 men and 15 normal women. SDR5A1 and SDR5A2 expression was estimated by RT-PCR using the gene of the ubiquitously expressed protein ß2-microglobulin as an internal control. The results are expressed as arbitrary units in relation to ß2-microglobulin absorbance (mean ± SEM). SDR5A2 expression was not detected in any hair samples analyzed in this study. No differences were found in SDR5A1 mRNA levels between men and normal women (0.78 ± 0.05 vs 0.74 ± 0.06, respectively)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hair follicle; Hirsutism; 5alpha-Reductase; Polycystic ovary syndrome.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000024

Serum cytokine levels in autoimmune and non-autoimmune hyperthyroid states
Provedor de dados: 56
Autores: Ward,L.S.; Fernandes,G.A.
Although the role of interleukin-2 (IL-2) and interferon gamma (&lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt;IFN) is still poorly understood in hyperthyroid diseases, it is reasonable to assume that these cytokines may be present at higher levels in Graves' disease (GD) than in other primarily non-autoimmune thyroid diseases. In order to look for an easy method to distinguish GD from primarily non-autoimmune causes of hyperthyroidism, we compared 13 healthy individuals with 21 treated and untreated hyperthyroid GD patients and with 19 patients with hyperthyroidism due to other etiologies: 7 cases of multinodular goiter, 5 cases of excessive hormone replacement and 7 cases of amiodarone-associated hyperthyroidism. All patients presented low TSH levels and a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytokines; IL-2; Hyperthyroidism; Graves' disease; Diagnosis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100009

Decorin is one of the proteoglycans expressed in Walker 256 rat mammary carcinoma
Provedor de dados: 56
Autores: Oba-Shinjo,S.M.; Berto,A.G.A.; Passerotti,C.C.; Barbosa,C.D.; Sampaio,L.O.
Proteoglycan and glycosaminoglycan content was analyzed in a model of rat mammary carcinoma to study the roles of these compounds in tumorigenesis. Hyaluronic acid and proteoglycans bearing chondroitin and/or dermatan sulfate chains were detected in solid tumors obtained after subcutaneous inoculation of Walker 256 rat carcinoma cells. About 10% of sulfated glycosaminoglycan chains corresponded to heparan sulfate. The small leucine-rich proteoglycan, decorin, was identified as one of the proteoglycans, in addition to others of higher molecular weight, by cross-reaction with an antiserum raised against pig laryngeal decorin and by N-terminal amino acid sequencing. Decorin was separated from other proteoglycans by hydrophobic chromatography and its complete...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proteoglycan; Glycosaminoglycan; Walker 256 tumor; Carcinoma.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800015

Rosmarinus officinalis L. increases Caenorhabditis elegans stress resistance and longevity in a DAF-16, HSF-1 and SKN-1-dependent manner
Provedor de dados: 56
Autores: Zamberlan,D.C.; Amaral,G.P.; Arantes,L.P.; Machado,M.L.; Mizdal,C.R.; Campos,M.M.A.; Soares,F.A.A.
Improving overall health and quality of life, preventing diseases and increasing life expectancy are key concerns in the field of public health. The search for antioxidants that can inhibit oxidative damage in cells has received a lot of attention. Rosmarinus officinalis L. represents an exceptionally rich source of bioactive compounds with pharmacological properties. In the present study, we explored the effects of the ethanolic extract of R. officinalis (eeRo) on stress resistance and longevity using the non-parasitic nematode Caenorhabditis elegans as a model. We report for the first time that eeRo increased resistance against oxidative and thermal stress and extended C. elegans longevity in an insulin/IGF signaling pathway-dependent manner. These data...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caenorhabditis elegans; Natural compounds; Rosemary; Daf-2; Stress resistance; Aging.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900601

Protective effect of maternal prenatal melatonin administration on rat pups born to mothers submitted to constant light during gestation
Provedor de dados: 56
Autores: Cisternas,C.D.; Compagnucci,M.V.; Conti,N.R.; Ponce,R.H.; Vermouth,N.T.
We studied the effects of adverse conditions such as constant light (LL) on the circadian rhythm of malate (MDH, EC 1.1.1.37) and lactate (LDH, EC 1.1.1.27) dehydrogenase activities of the testes of male Wistar rats on postnatal day 28 (PN28), anxiety-like behavior (elevated plus-maze test) at PN60 and sexual behavior at PN120. The rats were assigned to mother groups on day 10 of pregnancy: control (12-h light/dark), LL (light from day 10 to 21 of pregnancy), and LL+Mel (LL and sc injection to the mothers of a daily dose of melatonin, 1 mg/kg body weight at circadian time 12, from day 17 to 21 of pregnancy). LL offspring did not show circadian rhythms of MDH (N = 62) and LDH (N = 63) activities (cosinor and ANOVA-LSD Fisher). They presented a 44.7%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pineal hormone; Constant light; Entrainment; Copulatory behavior; Anxiety-like behavior.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900010

Effect of sexual steroids on the calcium content of aortic atherosclerotic plaque of oophorectomized rabbits
Provedor de dados: 56
Autores: Aldrighi,J.M.; Lanz,J.R.; Mansur,A.P.; Martinez,T.L.R.; Ramires,J.A.F.
We determined the effect of conjugated equine estrogen plus medroxyprogesterone acetate on calcium content of aortic atherosclerotic lesions in oophorectomized adult New Zealand rabbits submitted to a cholesterol rich diet. Five groups of 10 animals each were studied: G1 = control, G2 = cholesterol diet only, G3 = diet plus conjugated equine estrogen (0.625 mg/day); G4 and G5 = diet, conjugated equine estrogen (0.625 mg/day) plus medroxyprogesterone acetate (5 and 10 mg/day, respectively). Mean weight varied from 2.7 ± 0.27 to 3.1 ± 0.20 kg (P = 0.38) between groups at the beginning and 3.1 ± 0.27 to 3.5 ± 0.20 kg (P = 0.35) at the end of the experiment. Cholesterol and triglyceride levels were determined at the time of oophorectomy, 21 days after surgery...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estrogens; Progestagens; Calcium; Atherosclerosis; Oophorectomy; Aortic plaque area.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500007

Muscle strength but not functional capacity is associated with plasma interleukin-6 levels of community-dwelling elderly women
Provedor de dados: 56
Autores: Oliveira,D.M.G.; Narciso,F.M.S.; Santos,M.L.A.S.; Pereira,D.S.; Coelho,F.M.; Dias,J.M.D.; Pereira,L.S.M.
The association of plasma interleukin-6 (IL-6) levels, muscle strength and functional capacity was investigated in a cross-sectional study of community-dwelling elderly women from Belo Horizonte, Brazil. Elderly people who present controlled chronic diseases with no negative impact on physical, psychosocial and mental functionality are considered to be community-dwelling. Psychological and social stress due to unsuccessfully aging can represent a risk for immune system disfunctions. IL-6 levels, isokinetic muscle strength of knee flexion/extension, and functional tests to determine time required to rise from a chair and gait velocity were measured in 57 participants (71.21 ± 7.38 years). Serum levels of IL-6 were measured in duplicate and were performed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscle strength; Interleukin-6; Elderly women; Sarcopenia; Aging; IL-6.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200017

Candidate gene linkage analysis indicates genetic heterogeneity in Marfan syndrome
Provedor de dados: 56
Autores: Teixeira,L.V.S.; Mandelbaum,K.L.; Pereira,L.V.; Perez,A.B.A.
Marfan syndrome (MFS) is an autosomal dominant disease of the connective tissue that affects the ocular, skeletal and cardiovascular systems, with a wide clinical variability. Although mutations in the FBN1 gene have been recognized as the cause of the disease, more recently other loci have been associated with MFS, indicating the genetic heterogeneity of this disease. We addressed the issue of genetic heterogeneity in MFS by performing linkage analysis of the FBN1 and TGFBR2 genes in 34 families (345 subjects) who met the clinical diagnostic criteria for the disease according to Ghent. Using a total of six microsatellite markers, we found that linkage with the FBN1 gene was observed or not excluded in 70.6% (24/34) of the families, and in 1 family the MFS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marfan syndrome Fibrillin-1; TGF-β Genetic heterogeneity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800009

Adenosine A1 receptor-mediated inhibition of in vitro prolactin secretion from the rat anterior pituitary
Provedor de dados: 56
Autores: Picanço-Diniz,D.L.W.; Valença,M.M.; Antunes-Rodrigues,J.
In previous studies, we demonstrated biphasic purinergic effects on prolactin (PRL) secretion stimulated by an adenosine A2 agonist. In the present study, we investigated the role of the activation of adenosine A1 receptors by (R)-N6-(2-phenylisopropyl)adenosine (R-PIA) at the pituitary level in in vitro PRL secretion. Hemipituitaries (one per cuvette in five replicates) from adult male rats were incubated. Administration of R-PIA (0.001, 0.01, 0.1, 1, and 10 µM) induced a reduction of PRL secretion into the medium in a U-shaped dose-response curve. The maximal reduction was obtained with 0.1 µM R-PIA (mean ± SEM, 36.01 ± 5.53 ng/mg tissue weight (t.w.)) treatment compared to control (264.56 ± 15.46 ng/mg t.w.). R-PIA inhibition (0.01 µM = 141.97 ± 15.79...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclopentyltheophylline; Dibutyryl cAMP; R-PIA; Pertussis toxin; Cholera toxin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100013

Alanyl-glutamine protects the intestinal barrier function in trained rats against the impact of acute exhaustive exercise
Provedor de dados: 56
Autores: Freitas,A.K.L.; Silva,M.T.B.; Silva,C.M.S.; Prata,M.M.G.; Rodrigues,F.A.P.; Siqueira,R.J.B.; Lima,A.A.M.; Santos,A.A.; Havt,A.
Strenuous exercise triggers deleterious effects on the intestinal epithelium, but their mechanisms are still uncertain. Here, we investigated whether a prolonged training and an additional exhaustive training protocol alter intestinal permeability and the putative effect of alanyl-glutamine (AG) pretreatment in this condition. Rats were allocated into 5 different groups: 1) sedentary; 2 and 3) trained (50 min per day, 5 days per week for 12 weeks) with or without 6 weeks oral (1.5 g/kg) AG supplementation; 4 and 5) trained and subjected to an additional exhaustive test protocol with or without oral AG supplementation. Venous blood samples were collected to determine gasometrical indices at the end of the 12-week protocol or after exhaustive test. Lactate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Claudin-2; Intestinal permeability; Lactulose-mannitol test; Physical exercise; Tight junctions.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500602

The chronic blockade of angiotensin I-converting enzyme eliminates the sex differences of serum cytokine levels of spontaneously hypertensive rats
Provedor de dados: 56
Autores: Dalpiaz,P.L.M.; Lamas,A.Z.; Caliman,I.F.; Medeiros,A.R.S.; Abreu,G.R.; Moysés,M.R.; Andrade,T.U.; Alves,M.F.; Carmona,A.K.; Bissoli,N.S.
Sex hormones modulate the action of both cytokines and the renin-angiotensin system. However, the effects of angiotensin I-converting enzyme (ACE) on the proinflammatory and anti-inflammatory cytokine levels in male and female spontaneously hypertensive rats (SHR) are unclear. We determined the relationship between ACE activity, cytokine levels and sex differences in SHR. Female (F) and male (M) SHR were divided into 4 experimental groups each (n = 7): sham + vehicle (SV), sham + enalapril (10 mg/kg body weight by gavage), castrated + vehicle, and castrated + enalapril. Treatment began 21 days after castration and continued for 30 days. Serum cytokine levels (ELISA) and ACE activity (fluorimetry) were measured. Male rats exhibited a higher serum ACE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SHR Enalapril Sexual dimorphism Angiotensin I-converting enzyme Cytokines IL-10; IL-6 and TNF-α.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200171

Serotonin induces or inhibits neuritic regeneration of leech CNS neurons depending on neuronal identity
Provedor de dados: 56
Autores: Vargas,J.; Alfaro-Rodríguez,A.; Perez-Orive,J.
Recovery of motor function after central nervous system (CNS) injury is dependent on the regeneration capacity of the nervous system, which is a multifactorial process influenced, among other things, by the role of neuromodulators such as serotonin. The neurotransmitter serotonin can promote neuronal regeneration but there are also reports of it causing restriction, so it is important to clarify these divergent findings in order to understand the direct scope and side effects of potential pharmacological treatments. We evaluated the effect of serotonin on the extent of neuritic outgrowth and morphology of three different neuronal types in the leech Haementeria officinalis during their regeneration in vitro: Retzius interneurons (Rz), annulus erector (AE)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuronal regeneration; Serotonin; Leech; Neuronal culture; Pharmacological treatment.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200611

Interleukin-12 as a biomarker of the beneficial effects of food restriction in mice receiving high fat diet or high carbohydrate diet
Provedor de dados: 56
Autores: de Almeida-Souza,C.B.; Antunes,M.M.; Godoy,G.; Schamber,C.R.; Silva,M.A.R.C.P.; Bazotte,R.B.
The impact of food restriction (FR) during 56 days on serum levels of cytokines in mice fed a high-fat diet (HFD) or high-carbohydrate diet (HCD) were evaluated. The amount of food was reduced 50% for HFD-FR and HCD-FR groups compared to mice receiving free access to HFD (HFD group) or HCD (HCD group). We quantified the serum levels of basic fibroblast growth factor, granulocyte-macrophage colony-stimulating factor, inducible protein 10, interferon γ, interleukin 1α (IL-1α), IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17, keratinocyte chemoattractant, macrophage inflammatory protein-1α, monocyte chemotactic protein 1, monokine induced by IFN-γ, and tumor necrosis factor α. Only IL-12 levels were lower (P&lt;0.05), for both HFD-FR (HFD-FR vs HFD)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pro-inflammatory cytokines; Anti-inflammatory cytokines; Serum cytokines; Caloric restriction; Nutrition.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200606

Role of non-coding RNAs in non-aging-related neurological disorders
Provedor de dados: 56
Autores: Vieira,A.S.; Dogini,D.B.; Lopes-Cendes,I.
Protein coding sequences represent only 2% of the human genome. Recent advances have demonstrated that a significant portion of the genome is actively transcribed as non-coding RNA molecules. These non-coding RNAs are emerging as key players in the regulation of biological processes, and act as "fine-tuners" of gene expression. Neurological disorders are caused by a wide range of genetic mutations, epigenetic and environmental factors, and the exact pathophysiology of many of these conditions is still unknown. It is currently recognized that dysregulations in the expression of non-coding RNAs are present in many neurological disorders and may be relevant in the mechanisms leading to disease. In addition, circulating non-coding RNAs are emerging as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNA; Gene regulation; Molecular biomarkers.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800301

Correlation between lactose absorption and the C/T-13910 and G/A-22018 mutations of the lactase-phlorizin hydrolase (LCT) gene in adult-type hypolactasia
Provedor de dados: 56
Autores: Bulhões,A.C.; Goldani,H.A.S.; Oliveira,F.S.; Matte,U.S.; Mazzuca,R.B.; Silveira,T.R.
The C/T-13910 mutation is the major factor responsible for the persistence of the lactase-phlorizin hydrolase (LCT) gene expression. Mutation G/A-22018 appears to be only in co-segregation with C/T-13910. The objective of the present study was to assess the presence of these two mutations in Brazilian individuals with and without lactose malabsorption diagnosed by the hydrogen breath test (HBT). Ten milk-tolerant and 10 milk-intolerant individuals underwent the HBT after oral ingestion of 50 g lactose (equivalent to 1 L of milk). Analyses for C/T-13910 and G/A-22018 mutations were performed using a PCR-based method. Primers were designed for this study based on the GenBank sequence. The CT/GA, CT/AA, and TT/AA genotypes (lactase persistence) were found in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrogen breath test; Lactase-phlorizin hydrolase; Lactose intolerance; Milk intolerance; LCT polymorphism.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100004

Identification of hemolytic and neuroactive fractions in the venom of the sea anemone Bunodosoma cangicum
Provedor de dados: 56
Autores: Lagos,P.; Duran,R.; Cerveñansky,C.; Freitas,J.C.; Silveira,R.
Sea anemones are a rich source of biologically active substances. In crayfish muscle fibers, Bunodosoma cangicum whole venom selectively blocks the I K(Ca) currents. In the present study, we report for the first time powerful hemolytic and neuroactive effects present in two different fractions obtained by gel-filtration chromatography from whole venom of B. cangicum. A cytolytic fraction (Bcg-2) with components of molecular mass ranging from 8 to 18 kDa elicited hemolysis of mouse erythrocytes with an EC50 = 14 µg/ml and a maximum dose of 22 µg/ml. The effects of the neuroactive fraction, Bcg-3 (2 to 5 kDa), were studied on isolated crab nerves. This fraction prolonged the compound action potentials by increasing their duration and rise time in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bunodosoma cangicum; Sea anemone; Hemolytic activity; Neurotoxins; Crab nerve.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700009

Protective effect of yerba mate intake on the cardiovascular system: a post hoc analysis study in postmenopausal women
Provedor de dados: 56
Autores: da Veiga,D.T.A.; Bringhenti,R.; Copes,R.; Tatsch,E.; Moresco,R.N.; Comim,F.V.; Premaor,M.O.
The prevalence of cardiovascular and metabolic diseases is increased in postmenopausal women, which contributes to the burden of illnesses in this period of life. Yerba mate (Ilex paraguariensis) is a native bush from Southern South America. Its leaves are rich in phenolic components, which may have antioxidant, vasodilating, hypocholesterolemic, and hypoglycemic proprieties. This post hoc analysis of the case-control study nested in the Obesity and Bone Fracture Cohort evaluated the consumption of yerba mate and the prevalence of hypertension, dyslipidemia, and coronary diseases in postmenopausal women. Ninety-five postmenopausal women were included in this analysis. A questionnaire was applied to evaluate the risk factors and diagnosis of cardiovascular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yerba mate; Ilex paraguariensis; Postmenopausal women; Diabetes mellitus; Hypertension; Cardiovascular disease.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600607

Immunization against the colonization factor antigen I of enterotoxigenic Escherichia coli by administration of a bivalent Salmonella typhimurium aroA strain
Provedor de dados: 56
Autores: Guillobel,H.C.R.; Luna,M.G.; Camacho,E.F.; Almeida,D.F.; Ferreira,L.C.S.
An expression plasmid (pCFA-1) carrying the cfaB gene that codes for the enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin colonization factor antigen I (CFA/I) subunit was constructed and used to transform a derivative of the attenuated Salmonella typhimurium aroA vaccine strain SL3261 carrying an F'lacIq. Treatment of the transformed strain with isopropyl-ß-D-thiogalactopyranoside (IPTG) resulted in elevated in vitro expression of the CFA/I subunit. Although flagellar function and lipopolysaccharide (LPS) synthesis were similar in both the parental and the recombinant strains, spleen colonization was reduced in the recombinant strain. All BALB/c mice parenterally inoculated with the recombinant strain developed significant anti-CFA/I and anti-LPS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella typhimurium aroA; Enterotoxigenic Escherichia coli; Attenuated vaccines; Colonization factor antigen I.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400012

Hemodynamic responses to acute aortic coarctation in conscious sinoaortic denervated rats
Provedor de dados: 56
Autores: Fazan Jr.,R.; Machado,B.H.; Salgado,H.C.
The hemodynamic responses to acute (45 min) partial aortic constriction were studied in conscious intact (N = 7) or sinoaortic denervated (SAD) adult male Wistar rats (280-350 g, N = 7) implanted with carotid and femoral arterial catheters, a pneumatic cuff around the abdominal aorta and a pulsed Doppler flow probe to measure changes in aortic resistance. In addition, the hypertensive response and the reflex bradycardia elicited by total (N = 8) vs partial (N = 7) aortic constriction (monitored by maintenance of the pressure distal to the cuff at 50 mmHg) were compared in two other groups of intact rats. Intact rats presented a smaller hypertensive response (26 to 40% above basal level) to partial aortic constriction than SAD rats (38 to 58%). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Aortic coarctation; Doppler flowmetry; Aortic resistance; Reflex bradycardia; Sinoaortic denervation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000018

Comparison of Pavlovian serial conditional discrimination in rats and hamsters in the same experimental situation
Provedor de dados: 56
Autores: Bueno,J.L.O.; Figueiredo,T.H.; Mello,F.M.C.
The present study compares behavioral changes between two distinct rodent groups, hamsters (Mesocricetus auratus) and Wistar rats, when submitted in the same homogeneous experimental situations to a serial conditional discrimination procedure which involves water deprivation and the processing of temporal variables. Both hamsters and rats acquired serial positive conditional discrimination as indicated by higher frequencies of magazine-oriented behavior during the tone followed by reinforcement (T+) and preceded by the feature stimulus light (L) and during the empty interval, than during the tone alone not followed by reinforcement (T-). Rats' frequencies of magazine-oriented behavior were high during T+ and T-, initially during training, and decreased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conditional discrimination; Hamsters; Wistar rats; Behavior repertoire.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200012

Angiotensin-converting enzyme inhibition by lisinopril enhances liver regeneration in rats
Provedor de dados: 56
Autores: Ramalho,F.S.; Ramalho,L.N.Z.; Castro-e-Silva Júnior,O.; Zucoloto,S.; Corrêa,F.M.A.
Bradykinin has been reported to act as a growth factor for fibroblasts, mesangial cells and keratinocytes. Recently, we reported that bradykinin augments liver regeneration after partial hepatectomy in rats. Angiotensin-converting enzyme (ACE) is also a powerful bradykinin-degrading enzyme. We have investigated the effect of ACE inhibition by lisinopril on liver regeneration after partial hepatectomy. Adult male Wistar rats underwent 70% partial hepatectomy (PH). The animals received lisinopril at a dose of 1 mg kg body weight-1 day-1, or saline solution, intraperitoneally, for 5 days before hepatectomy, and daily after surgery. Four to six animals from the lisinopril and saline groups were sacrificed at 12, 24, 36, 48, 72, and 120 h after PH. Liver...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Angiotensin-converting enzyme inhibitors; Lisinopril; Bradykinin; Liver regeneration; Cell proliferation; Hepatocyte.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100016

Changes in red blood cell osmotic fragility induced by total plasma and plasma fractions obtained from rats bearing progressive and regressive variants of the Walker 256 tumor
Provedor de dados: 56
Autores: Cavalcanti,T.C.; Gregorini,C.C.; Guimarães,F.; Rettori,O.; Vieira-Matos,A.N.
Two variants (A and B) of the widely employed Walker 256 rat tumor cells are known. When inoculated sc, the A variant produces solid, invasive, highly metastasizing tumors that cause severe systemic effects and death. We have obtained a regressive variant (AR) whose sc growth is slower, resulting in 70-80% regression followed by development of immunity against A and AR variants. Simultaneously with the beginning of tumor regression, a temporary anemia developed (~8 days duration), accompanied by marked splenomegaly (~300%) and changes in red blood cell osmotic fragility, with mean corpuscular fragility increasing from 4.1 to 6.5 g/l NaCl. The possibility was raised that plasma factors associated with the immune response induced these changes. In the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plasma fractions; Temporary anemia; RBC osmotic fragility; Tumor regressive variant; Cancer immunity; Walker 256 tumor.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700009

Proton magnetic resonance spectroscopy of the frontal, cingulate and perirolandic cortices and its relationship to skin conductance in patients with schizophrenia
Provedor de dados: 56
Autores: Sanches,R.F.; Crippa,J.A.S.; Hallak,J.E.C.; de Sousa,J.P.M.; Araújo,D.; Santos,A.C.; Zuardi,A.W.
The aim of the present study was to determine whether specific subgroups of schizophrenic patients, grouped according to electrodermal characteristics, show differences in the N-acetylaspartate/creatine plus choline (NAA / (Cr + Cho)) ratios in the frontal, cingulate and perirolandic cortices. Skin conductance levels (SCL) and skin conductance responses to auditory stimulation were measured in 38 patients with schizophrenia and in the same number of matched healthy volunteers (control). All subjects were submitted to multivoxel proton magnetic resonance spectroscopic imaging. When compared to the control group, patients presented significantly lower NAA / (Cr + Cho) ratios in the right dorsolateral prefrontal cortex (schizophrenia = 0.95 ± 0.03; control =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnetic resonance spectroscopy; Skin conductance; Schizophrenia; N-acetylaspartate; Cingulate region; Dorsolateral prefrontal cortex.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200015

Correction Braz J Med Biol Res 2004; 37(10): 1551-1556 Effect of the aerobic capacity on the validity of the anaerobic threshold for determination of the maximal lactate steady state in cycling
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500012

Inactivated Pseudomonas aeruginosa inhibits hypoxia-induced pulmonary hypertension by preventing TGF-β1/Smad signaling
Provedor de dados: 56
Autores: Chai,S.D.; Liu,T.; Dong,M.F.; Li,Z.K.; Tang,P.Z.; Wang,J.T.; Ma,S.J.
Pseudomonas aeruginosa is one of the common colonizing bacteria of the human body and is an opportunistic pathogen frequently associated with respiratory infections. Inactivated P. aeruginosa (IPA) have a variety of biological effects against inflammation and allergy. Transforming growth factor-β (TGF-β) signaling plays a critical role in the regulation of cell growth, differentiation, and development in a wide range of biological systems. The present study was designed to investigate the effects of IPA on TGF-β/Smad signaling in vivo, using a hypoxia-induced pulmonary hypertension (PH) rat model. Sprague Dawley rats (n=40) were exposed to 10% oxygen for 21 days to induce PH. At the same time, IPA was administered intravenously from day 1 to day 14. Mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inactivated Pseudomonas aeruginosa; Pulmonary hypertension; TGF-β/Smad; Primary arterial smooth muscle cells; Α-smooth muscle actin.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000601

Use of erythromycin for the treatment of severe chronic constipation in children
Provedor de dados: 56
Autores: Bellomo-Brandão,M.A.; Collares,E.F.; da-Costa-Pinto,E.A.L.
The efficacy of erythromycin was assessed in the treatment of 14 children aged 4 to 13 years with refractory chronic constipation, and presenting megarectum and fecal impaction. A double-blind, placebo- controlled, crossover study was conducted at the Pediatric Gastroenterology Outpatient Clinic of the University Hospital. The patients were randomized to receive placebo for 4 weeks followed by erythromycin estolate, 20 mg kg-1 day-1, divided into four oral doses for another 4 weeks, or vice versa. Patient outcome was assessed according to a clinical score from 12 (most severe clinical condition) to 0 (complete recovery). At enrollment in the study and on the occasion of follow-up medical visits at two-week intervals, patient score and laxative requirements...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gastrointestinal motility; Constipation; Children; Prokinetics; Erythromycin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000016

Factors associated with bacteremia due to multidrug-resistant Gram-negative bacilli in hematopoietic stem cell transplant recipients
Provedor de dados: 56
Autores: Garnica,M.; Maiolino,A.; Nucci,M.
The epidemiology of bacteremia developing during neutropenia has changed in the past decade, with the re-emergence of Gram-negative (GN) bacteria and the development of multidrug resistance (MDR) among GN bacteria. We conducted a case-control study in order to identify factors associated with bacteremia due to multidrug-resistant Gram-negative (MDRGN) isolates in hematopoietic stem cell transplant recipients. Ten patients with MDRGN bacteremia were compared with 44 patients with GN bacteremia without MDR. Bacteremia due to Burkholderia or Stenotrophomonas sp was excluded from analysis (3 cases), because the possibility of intrinsical resistance. Infection due to MDRGN bacteria occurred in 2.9% of 342 hematopoietic stem cell transplant recipients....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hematopoietic stem cell transplant; Neutropenia; Gram-negative bacteria; Antibiotic resistance; Risk factors.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300010

Effects of acupuncture on behavioral, cardiovascular and hormonal responses in restraint-stressed Wistar rats
Provedor de dados: 56
Autores: Guimarães,C.M.; Pinge,M.C.M.; Yamamura,Y.; Mello,L.E.A.M.
Stress is a well-known entity and may be defined as a threat to the homeostasis of a being. In the present study, we evaluated the effects of acupuncture on the physiological responses induced by restraint stress. Acupuncture is an ancient therapeutic technique which is used in the treatment and prevention of diseases. Its proposed mechanisms of action are based on the principle of homeostasis. Adult male Wistar EPM-1 rats were divided into four groups: group I (N = 12), unrestrained rats with cannulas previously implanted into their femoral arteries for blood pressure and heart rate measurements; group II (N = 12), rats that were also cannulated and were submitted to 60-min immobilization; group III (N = 12), same as group II but with acupuncture needles...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress; Acupuncture; Catecholamines; Corticosterone.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200011

Mechanisms of endothelial dysfunction in obesity-associated hypertension
Provedor de dados: 56
Autores: Lobato,N.S.; Filgueira,F.P.; Akamine,E.H.; Tostes,R.C.; Carvalho,M.H.C.; Fortes,Z.B.
Obesity is strongly associated with high blood pressure, dyslipidemia, and type 2 diabetes. These conditions synergistically increase the risk of cardiovascular events. A number of central and peripheral abnormalities can explain the development or maintenance of high blood pressure in obesity. Of great interest is endothelial dysfunction, considered to be a primary risk factor in the development of hypertension. Additional mechanisms also related to endothelial dysfunction have been proposed to mediate the development of hypertension in obese individuals. These include: increase in both peripheral vasoconstriction and renal tubular sodium reabsorption, increased sympathetic activity and overactivation of both the renin-angiotensin system and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Obesity; Endothelial dysfunction; Oxidative stress; Renin-angiotensin system; Nitric oxide.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500003

Genetic association of SNPs in the FTO gene and predisposition to obesity in Malaysian Malays
Provedor de dados: 56
Autores: Apalasamy,Y.D.; Ming,M.F.; Rampal,S.; Bulgiba,A.; Mohamed,Z.
The common variants in the fat mass- and obesity-associated (FTO) gene have been previously found to be associated with obesity in various adult populations. The objective of the present study was to investigate whether the single nucleotide polymorphisms (SNPs) and linkage disequilibrium (LD) blocks in various regions of the FTO gene are associated with predisposition to obesity in Malaysian Malays. Thirty-one FTO SNPs were genotyped in 587 (158 obese and 429 non-obese) Malaysian Malay subjects. Obesity traits and lipid profiles were measured and single-marker association testing, LD testing, and haplotype association analysis were performed. LD analysis of the FTO SNPs revealed the presence of 57 regions with complete LD (D’ = 1.0). In addition, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FTO gene; SNP; Obesity; Linkage disequilibrium; Haplotypes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200003

Photic driving in the electroencephalogram of children and adolescents: harmonic structure and relation to the resting state
Provedor de dados: 56
Autores: Lazarev,V.V.; Simpson,D.M.; Schubsky,B.M.; deAzevedo,L.C.
In order to identify latent bioelectrical oscillators, 15 normal subjects (aged 9-17 years, 8 males, 7 females) were subjected to intermittent photic stimulation. The EEG amplitude spectra corresponding to the 11 fixed frequencies of stimulation presented (3-24 Hz) were combined to form "profiles" of the driving reaction in the right occipital area. The driving response varied with frequency, and was demonstrable in 70-100% of cases (using as criterion peak amplitudes 20% larger than those of the neighbors). The strongest responses were observed at the frequency closest to the alpha peak of the resting EEG. A secondary profile maximum was in the theta band. In 10 subjects, this maximum exceeded half the alpha peak (with an average of 72.4% of the alpha...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electroencephalogram; Intermittent photic stimulation; Photic driving; Harmonic; Children; Adolescents.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200010

Antiviral activity of shikonin ester derivative PMM-034 against enterovirus 71 in vitro
Provedor de dados: 56
Autores: Zhang,Y.; Han,H.; Sun,L.; Qiu,H.; Lin,H.; Yu,L.; Zhu,W.; Qi,J.; Yang,R.; Pang,Y.; Wang,X.; Lu,G.; Yang,Y.
Human enterovirus 71 (EV71) is the major causative agent of hand, foot, and mouth disease (HFMD), particularly in infants and children below 4 years of age. Shikonin is a bioactive compound with anti-inflammatory, antiviral, and antibacterial activities derived from the roots of the Chinese medicinal herb Lithospermum erythrorhizon. This study aimed to examine the antiviral activity of PMM-034, a shikonin ester derivative, against EV71 in rhabdomyosarcoma (RD) cells. Cytotoxicity of PMM-034 on RD cells was determined using WST-1 assay. Dose- and time-dependent effects of PMM-034 on EV71 replication in RD cells were determined using plaque reduction assay. mRNA expression levels of EV71/VP1 and pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EV71; VP1; Shikonin ester derivatives PMM-034; Rhabdomyosarcoma cells; NF-κB.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000602

Association of MICA gene polymorphisms with liver fibrosis in schistosomiasis patients in the Dongting Lake region
Provedor de dados: 56
Autores: Gong,Zheng; Luo,Qi-Zhi; Lin,Lin; Su,Yu-Ping; Peng,Hai-Bo; Du,Kun; Yu,Ping; Wang,Shi-Ping.
Major histocompatibility complex class I chain-related A (MICA) is a highly polymorphic gene located within the MHC class I region of the human genome. Expressed as a cell surface glycoprotein, MICA modulates immune surveillance by binding to its cognate receptor on natural killer cells, NKG2D, and its genetic polymorphisms have been recently associated with susceptibility to some infectious diseases. We determined whether MICA polymorphisms were associated with the high rate of Schistosoma parasitic worm infection or severity of disease outcome in the Dongting Lake region of Hunan Province, China. Polymerase chain reaction-sequence specific priming (PCR-SSP) and sequencing-based typing (SBT) were applied for high-resolution allele typing of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosoma japonicum; MICA; NKG2D; Gene polymorphism; Liver fibrosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300007

Pituitary glycoprotein hormone a-subunit secretion by cirrhotic patients
Provedor de dados: 56
Autores: Oliveira,M.C.; Pizarro,C.B.; Cassal,A.; Cremonese,R.; Vieira,J.G.H.
Secretion of the &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;-subunit of pituitary glycoprotein hormones usually follows the secretion of intact gonadotropins and is increased in gonadal failure and decreased in isolated gonadotropin deficiency. The aim of the present study was to determine the levels of the &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;-subunit in the serum of patients with cirrhosis of the liver and to compare the results obtained for eugonadal cirrhotic patients with those obtained for cirrhotic patients with hypogonadotropic hypogonadism. Forty-seven of 63 patients with cirrhosis (74.6%) presented hypogonadism (which was central in 45 cases and primary in 2), 7 were eugonadal, and 9 women were in normal menopause. The serum &lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: &lt; FONT FACE=Symbol&gt; A&lt; /font&gt; -subunit pituitary gonadotropins hypogonadism cirrhosis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100011

Decision support system for the diagnosis of schizophrenia disorders
Provedor de dados: 56
Autores: Razzouk,D.; Mari,J.J.; Shirakawa,I.; Wainer,J.; Sigulem,D.
Clinical decision support systems are useful tools for assisting physicians to diagnose complex illnesses. Schizophrenia is a complex, heterogeneous and incapacitating mental disorder that should be detected as early as possible to avoid a most serious outcome. These artificial intelligence systems might be useful in the early detection of schizophrenia disorder. The objective of the present study was to describe the development of such a clinical decision support system for the diagnosis of schizophrenia spectrum disorders (SADDESQ). The development of this system is described in four stages: knowledge acquisition, knowledge organization, the development of a computer-assisted model, and the evaluation of the system's performance. The knowledge was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clinical decision support systems; Artificial intelligence; Decision making; Expert systems; Schizophrenia; Medical informatics.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100014

CPU0213, a novel endothelin type A and type B receptor antagonist, protects against myocardial ischemia/reperfusion injury in rats
Provedor de dados: 56
Autores: Wang,Z.Y.; Zhang,W.; Li,X.Z.; Han,Y.; Chen,Y.P.; Liu,Z.; Xie,L.P.; Ji,Y.; Lu,X.
The efficacy of endothelin receptor antagonists in protecting against myocardial ischemia/reperfusion (I/R) injury is controversial, and the mechanisms remain unclear. The aim of this study was to investigate the effects of CPU0123, a novel endothelin type A and type B receptor antagonist, on myocardial I/R injury and to explore the mechanisms involved. Male Sprague-Dawley rats weighing 200-250 g were randomized to three groups (6-7 per group): group 1, Sham; group 2, I/R + vehicle. Rats were subjected to in vivo myocardial I/R injury by ligation of the left anterior descending coronary artery and 0.5% sodium carboxymethyl cellulose (1 mL/kg) was injected intraperitoneally immediately prior to coronary occlusion. Group 3, I/R + CPU0213. Rats were subjected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelin receptor antagonist; Myocardial ischemia/reperfusion injury; Akt; ENOS.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100011

Differential effects of aging on spatial contrast sensitivity to linear and polar sine-wave gratings
Provedor de dados: 56
Autores: Costa,T.L.; Nogueira,R.M.T.B.L.; Pereira,A.G.F.; Santos,N.A.
Changes in visual function beyond high-contrast acuity are known to take place during normal aging. We determined whether sensitivity to linear sine-wave gratings and to an elementary stimulus preferentially processed in extrastriate areas could be distinctively affected by aging. We measured spatial contrast sensitivity twice for concentric polar (Bessel) and vertical linear gratings of 0.6, 2.5, 5, and 20 cycles per degree (cpd) in two age groups (20-30 and 60-70 years). All participants were free of identifiable ocular disease and had normal or corrected-to-normal visual acuity. Participants were more sensitive to Cartesian than to polar gratings in all frequencies tested, and the younger adult group was more sensitive to all stimuli tested. Significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contrast sensitivity; Circular gratings; Linear gratings; Aging; Spatial vision.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000855

The epigenetic modifiers 5-aza-2'-deoxycytidine and trichostatin A influence adipocyte differentiation in human mesenchymal stem cells
Provedor de dados: 56
Autores: Zych,J.; Stimamiglio,M.A.; Senegaglia,A.C.; Brofman,P.R.S.; Dallagiovanna,B.; Goldenberg,S.; Correa,A.
Epigenetic mechanisms such as DNA methylation and histone modification are important in stem cell differentiation. Methylation is principally associated with transcriptional repression, and histone acetylation is correlated with an active chromatin state. We determined the effects of these epigenetic mechanisms on adipocyte differentiation in mesenchymal stem cells (MSCs) derived from bone marrow (BM-MSCs) and adipose tissue (ADSCs) using the chromatin-modifying agents trichostatin A (TSA), a histone deacetylase inhibitor, and 5-aza-2′-deoxycytidine (5azadC), a demethylating agent. Subconfluent MSC cultures were treated with 5, 50, or 500 nM TSA or with 1, 10, or 100 µM 5azadC for 2 days before the initiation of adipogenesis. The differentiation was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem cells; Trichostatin A; 5-Aza-2′-deoxycytidine; Adipocyte differentiation; Epigenetics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500405

A newly designed intensive caregiver education program reduces cognitive impairment, anxiety, and depression in patients with acute ischemic stroke
Provedor de dados: 56
Autores: Zhang,Li; Zhang,Tianzhu; Sun,Yan.
This study aimed to evaluate the effect of a newly designed intensive caregiver education program (ICEP) on reducing cognitive impairment, anxiety, and depression in acute ischemic stroke (AIS) patients. One hundred and ninety-six AIS patients were divided into ICEP group and Control group in a 1:1 ratio using blocked randomization method. In the ICEP group, the caregivers received ICEP, while in the Control group caregivers received usual education and guidance. All patients received conventional rehabilitation treatment. Cognitive impairment (assessed by Mini Mental State Examination (MMSE) score and Montreal Cognitive Assessment (MoCA) score), anxiety (assessed by Hospital Anxiety and Depression Scale (HADS)-A score and Self-rating Anxiety Scale (SAS)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute ischemic stroke; Intensive caregiver education program; Cognitive impairment; Anxiety; Depression.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900609

Control of the adrenocortical cell cycle: interaction between FGF2 and ACTH
Provedor de dados: 56
Autores: Armelin,H.A.; Lotfi,C.F.P.
FGF2 elicits a strong mitogenic response in the mouse Y-1 adrenocortical tumor cell line, that includes a rapid and transient activation of the ERK-MAPK cascade and induction of the c-Fos protein. ACTH, itself a very weak mitogen, blocks the mitogenic response effect of FGF2 in the early and middle G1 phase, keeping both ERK-MAPK activation and c-Fos induction at maximal levels. Probing the mitogenic response of Y-1 cells to FGF2 with ACTH is likely to uncover reactions underlying the effects of this hormone on adrenocortical cell growth.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenocortical cell cycle; ACTH; FGF2; ERK-MAPK; C-fos proto-oncogene.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700007

Analysis of the DF508 mutation in a Brazilian cystic fibrosis population: comparison of pulmonary status of homozygotes with other patients
Provedor de dados: 56
Autores: Maróstica,P.J.C.; Raskin,S.; Abreu-e-Silva,F.A.
Sixty-one cystic fibrosis patients admitted for check-up or antibiotic treatment were enrolled for genetic and clinical evaluation. Genetic analysis was performed on blood samples stored on neonatal screening cards using PCR techniques to determine the presence of &lt;FONT FACE="Symbol"&gt;D&lt;/font&gt;F508 mutations. Clinical evaluation included Shwachman and Chrispin-Norman scores, age at onset of symptoms and diagnosis, spirometry, awake and sleep pulse oximetry, hyponychial angle measurement and presence of chronic Pseudomonas aeruginosa colonization. Eighteen patients (29.5%) were homozygous for the &lt;FONT FACE="Symbol"&gt;D&lt;/font&gt;F508 mutation, 26 (42.6%) had one &lt;FONT FACE="Symbol"&gt;D&lt;/font&gt;F508 mutation and 17 (27.9%) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cystic fibrosis &lt; FONT FACE=Symbol&gt; D&lt; /font&gt; F508 gene mutation genotype phenotype.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400009

Impact of enhanced recovery after surgery on postoperative rehabilitation, inflammation, and immunity in gastric carcinoma patients: a randomized clinical trial
Provedor de dados: 56
Autores: Wang,Wu-Ke; Tu,Chao-Yong; Shao,Chu-Xiao; Chen,Wei; Zhou,Qing-Yun; Zhu,Jing-De; Xu,Hong-Tao.
We determined the effects of enhanced recovery after surgery (ERAS) in patients undergoing radical surgery for gastric carcinoma. Sixty patients undergoing radical gastrectomy for gastric carcinoma in Lishui Hospital between March and October 2016 were randomized to receive either ERAS (30 patients) or conventional care (30 patients, controls). Clinical, economic, and laboratory indices were analyzed. ERAS patients showed faster recovery and shorter postoperative hospital stays than the controls (P&lt;0.05). Some clinical indices (i.e., time to first flatus and defecation, time to removal of drainage tubes, time to resumption of oral feeding, time to postoperative mobilization, and postoperative complications) were significantly better in ERAS patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enhanced recovery after surgery; Perioperative period; Gastric carcinoma; Inflammatory response; Immune function.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500609

Agents that affect cAMP levels or protein kinase A activity modulate memory consolidation when injected into rat hippocampus but not amygdala
Provedor de dados: 56
Autores: Bevilaqua,L.; Ardenghi,P.; Schröder,N.; Bromberg,E.; Quevedo,J.; Schmitz,P.K.; Bianchin,M.; Walz,R.; Schaeffer,E.; Medina,J.H.; Izquierdo,I.
Male Wistar rats were trained in one-trial step-down inhibitory avoidance using a 0.4-mA footshock. At various times after training (0, 1.5, 3, 6 and 9 h for the animals implanted into the CA1 region of the hippocampus; 0 and 3 h for those implanted into the amygdala), these animals received microinfusions of SKF38393 (7.5 µg/side), SCH23390 (0.5 µg/side), norepinephrine (0.3 µg/side), timolol (0.3 µg/side), 8-OH-DPAT (2.5 µg/side), NAN-190 (2.5 µg/side), forskolin (0.5 µg/side), KT5720 (0.5 µg/side) or 8-Br-cAMP (1.25 µg/side). Rats were tested for retention 24 h after training. When given into the hippocampus 0 h post-training, norepinephrine enhanced memory whereas KT5720 was amnestic. When given 1.5 h after training, all treatments were ineffective....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Memory formation; Memory modulation; Hippocampus; Amygdala; CAMP/PKA/CREB-P pathway; Dopamine D; Receptors; SS-adrenoceptors; 5HT1A receptors.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800009

Effect of oral ingestion of an extract of the herb Uncaria tomentosa on the biodistribution of sodium pertechnetate in rats
Provedor de dados: 56
Autores: Moreno,S.R.F.; Silva,A.L.C.; Diré,G.; Honeycut,H.; Carvalho,J.J.; Nascimento,A.L.; Pereira,M.; Rocha,E.K.; Oliveira-Timóteo,M.; Arnobio,A.; Olej,B.; Bernardo-Filho,M.; Caldas,L.Q.A.
The aim of the present study was to determine the effect of the oral ingestion of an extract of the herb Uncaria tomentosa (cat's claw) on the biodistribution of the radiobiocomplex sodium pertechnetate (Na99mTcO4) in rats. The animals (male Wistar rats, 2 months old, 180-220 g), were treated (1 mL) with an U. tomentosa extract (32 mg/mL, N = 5) or 0.9% NaCl solution (control, N = 5) for 7 days. After this period, Na99mTcO4 (3.7 MBq, 0.3 mL) was injected through the ocular plexus and after 10 min the rats were killed, the organs isolated and counted in a well-gamma counter. A significant (P &lt; 0.05) alteration in Na99mTcO4 uptake i) from 0.57 ± 0.008 to 0.39 ± 0.06 %ATI/organ (P &lt; 0.05) and from 0.57 ± 0.17 to 0.39 ± 0.14 %ATI/g (P &lt; 0.05) was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Uncaria tomentosa; Biodistribution; Sodium pertechnetate; Radiobiocomplex.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100010

The Shiga toxin 2 B subunit inhibits net fluid absorption in human colon and elicits fluid accumulation in rat colon loops
Provedor de dados: 56
Autores: Creydt,V. Pistone; Miyakawa,M. Fernandez; Martín,F.; Zotta,E.; Silberstein,C.; Ibarra,C.
Shiga toxin (Stx)-producing Escherichia coli (STEC) colonizes the large intestine causing a spectrum of disorders, including watery diarrhea, bloody diarrhea (hemorrhagic colitis), and hemolytic-uremic syndrome. It is estimated that hemolytic-uremic syndrome is the most common cause of acute renal failure in infants in Argentina. Stx is a multimeric toxin composed of one A subunit and five B subunits. In this study we demonstrate that the Stx2 B subunit inhibits the water absorption (Jw) across the human and rat colonic mucosa without altering the electrical parameters measured as transepithelial potential difference and short circuit current. The time-course Jw inhibition by 400 ng/ml purified Stx2 B subunit was similar to that obtained using 12 ng/ml...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemolytic-uremic syndrome; Diarrhea; Shiga toxin 2; B subunit; Water transport.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600004

Activity of liver microsomal enzymes during the chronic phase of murine schistosomiasis
Provedor de dados: 56
Autores: Conte,F.P.; Fidalgo-Neto,A.A.; Manhães-Rocha,D.A.; Paumgartten,F.J.R.; De-Oliveira,A.C.A.X.
The effects of schistosomiasis on microsomal enzymes were studied on post-infection day 90 when accumulated damage and fibrosis are most intense but granulomatous reaction around the eggs harbored in the liver is smaller than during the earlier phases. Swiss Webster (SW) and DBA/2 mice of either sex (N = 12 per sex per group) were infected with 100 Schistosoma mansoni cercariae on postnatal day 10 and killed on post-infection day 90. Cytochrome P-450 (CYP) concentration and alkoxyresorufin-O-dealkylases (EROD, MROD, BROD, and PROD), p-nitrophenol-hydroxylase (PNPH), coumarin-7-hydroxylase (COH), and UDP-glucuronosyltransferase (UGT) activities were measured in hepatic microsomes. Age-matched mice of the same sex and strain were used as controls. In S....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Schistosoma mansoni; Pharmacokinetics; Cytochrome P-450; Monooxygenases; Granuloma; Liver microsomes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500008

Low-dose thioperamide injected into the cerebellar vermis of mice immediately after exposure to the elevated plus-maze impairs their avoidance behavior on re-exposure to the apparatus
Provedor de dados: 56
Autores: Costa Neto,J.; Serafim,K.R.; Gianlorenço,A.C.L.; Mattioli,R.
The present study investigated the effect of thioperamide (THIO), an H3 histaminergic receptor antagonist, microinjected into the cerebellar vermis on emotional memory consolidation in male Swiss albino mice re-exposed to the elevated plus-maze (EPM). We implanted a guide cannula into the cerebellar vermis using stereotactic surgery. On the third day after surgery, we performed behavioral tests for two consecutive days. On the first day (exposure), the mice (n=10/group) were exposed to the EPM and received THIO (0.06, 0.3, or 1.5 ng/0.1 µL) immediately after the end of the session. Twenty-four hours later, the mice were re-exposed to the EPM under the same experimental conditions, but without drug injection. A reduction in the exploration of the open arms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histaminergic system; Cerebellum; H3; Emotional memory; Memory consolidation; Elevated plus-maze.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100943

Analgesic effects of Marasmius androsaceus mycelia ethanol extract and possible mechanisms in mice
Provedor de dados: 56
Autores: Song,Jia; Wang,Xue; Huang,Yu; Qu,Yidi; Zhang,Guirong; Wang,Di.
Marasmius androsaceus is a medicinal fungus mainly used to treat various forms of pain in China. This study investigated the analgesic effects of an ethanol extract of M. androsaceus (MAE) and its potential molecular mechanisms. Oral administration of MAE (50, 200, and 1000 mg/kg) had significant analgesic effects in an acid-induced writhing test, a formalin test, and a hot-plate test, with effectiveness similar to tramadol (the positive control drug). The autonomic activity test showed that MAE had no harmful effects on the central nervous system in mice. MAE resulted in significantly enhanced levels of noradrenalin and 5-hydroxytryptamine in serum but suppressed both of these neurotransmitters in the hypothalamus after 30 s of hot-plate stimulation....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marasmius androsaceus; Analgesic ability; Monoamine neurotransmitters; Calcium channels.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400613

Measurement of Ki-67 antigen in 159 pituitary adenomas using the MIB-1 monoclonal antibody
Provedor de dados: 56
Autores: Pizarro,C.B.; Oliveira,M.C.; Coutinho,L.B.; Ferreira,N.P.
Pituitary adenomas sometimes show rapid growth and recurrence, and about one third invade the structures surrounding the sella turcica. In an attempt to determine aggressive behavior at an early stage, we used the MIB-1 antibody to identify the Ki-67 antigen. The present study was designed to evaluate pituitary adenomatous tissue in terms of secretion and proliferation and to correlate the Ki-67 index with hormone phenotype and invasive behavior. Material from 159 patients submitted to one or more resections of pituitary adenomas was evaluated. Forty-two non-secretory adenomas and 43 adenomas immunoreactive for growth hormone, 19 for prolactin, 18 for growth hormone and prolactin, 16 for adrenocorticotropic hormone (ACTH), and 21 cases of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pituitary adenoma; Invasive adenoma; Ki-67 antigen; MIB-1 antibody; Cell proliferation; Immunohistochemistry.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200011

Tomato and garlic by gavage modulate 7,12-dimethylbenz[a]anthracene-induced genotoxicity and oxidative stress in mice
Provedor de dados: 56
Autores: Bhuvaneswari,V.; Velmurugan,B.; Abraham,S.K.; Nagini,S.
Chemoprotection by dietary agents is a promising strategy for cancer prevention. The aim of the present study was to evaluate the combined effect of tomato and garlic against 7,12-dimethylbenz- [a]anthracene (DMBA)-induced genetic damage and oxidative stress in 12-14-week-old male Swiss albino mice. The animals were randomized into experimental and control groups and divided into eight groups of five animals each. Group 1 animals were injected intraperitoneally with 35 mg/kg body weight DMBA suspended in peanut oil as a single dose. Groups 2-4 animals received tomato (500 mg/kg body weight), garlic (125 mg/kg body weight) and a combination of tomato and garlic for 5 days by gavage, respectively, followed by DMBA 1.5 h after the final feeding. The doses of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Antioxidants; DMBA; Garlic; Lipid peroxidation; Micronuclei; Tomato; Lycopene.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700012

Catecholamine-induced vasoconstriction is sensitive to carbonic anhydrase I activation
Provedor de dados: 56
Autores: Puscas,I.; Coltau,M.; Gilau,L.; Pasca,R.; Domuta,G.; Baican,M.; Hecht,A.
We studied the relationship between alpha- and beta-adrenergic agonists and the activity of carbonic anhydrase I and II in erythrocyte, clinical and vessel studies. Kinetic studies were performed. Adrenergic agonists increased erythrocyte carbonic anhydrase as follows: adrenaline by 75%, noradrenaline by 68%, isoprenaline by 55%, and orciprenaline by 62%. The kinetic data indicated a non-competitive mechanism of action. In clinical studies carbonic anhydrase I from erythrocytes increased by 87% after noradrenaline administration, by 71% after orciprenaline and by 82% after isoprenaline. The increase in carbonic anhydrase I paralleled the increase in blood pressure. Similar results were obtained in vessel studies on piglet vascular smooth muscle. We believe...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenergic agonists; Carbonic anhydrase; Arterial blood pressure.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300007

Construction of occluded recombinant baculoviruses containing the full-length cry1Ab and cry1Ac genes from Bacillus thuringiensis
Provedor de dados: 56
Autores: Ribeiro,B.M.; Crook,N.E.
The administration of baculoviruses to insects for bioassay purposes is carried out, in most cases, by contamination of food surfaces with a known amount of occlusion bodies (OBs). Since per os infection is the natural route of infection, occluded recombinant viruses containing crystal protein genes (cry1Ab and cry1Ac) from Bacillus thuringiensis were constructed for comparison with the baculovirus prototype Autographa californica nucleopolyhedrovirus (AcNPV). The transfer vector pAcUW2B was used for construction of occluded recombinant viruses. The transfer vector containing the crystal protein genes was cotransfected with linearized DNA from a non-occluded recombinant virus. The isolation of recombinant viruses was greatly facilitated by the reduction of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baculovirus; AcNPV; Bacillus thuringiensis cry1A; Heliothis virescens; Pieris brassicae.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600006

Resveratrol inhibits the intracellular calcium increase and angiotensin/endothelin system activation induced by soluble uric acid in mesangial cells
Provedor de dados: 56
Autores: Albertoni,G.; Schor,N.
Resveratrol (Resv) is natural polyphenol found in grapes. This study evaluated the protective effect of Resv against the effects of uric acid (UA) in immortalized human mesangial cells (ihMCs). ihMCs were preincubated with Resv (12.5 µM) for 1 h and treated with UA (10 mg/dL) for 6 or 12 h. The intracellular calcium concentration [Ca2+]i was quantified by fluorescence using flow cytometry. Angiotensinogen (AGT) and pre-pro endothelin-1 (ppET-1) mRNA were assayed by quantitative real-time RT-PCR. Angiotensin II (AII) and endothelin-1 (ET-1) were assayed by ELISA. UA significantly increased [Ca2+]i. Pre-incubation with Resv significantly reduced the change in [Ca2+]i induced by UA. Incubation with UA for 6 or 12 h also increased AGT mRNA expression and AII...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Resveratrol; Intracellular calcium; Angiotensin; Endothelin; Mesangial cells; Uric acid.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100051

Sawtooth waves during REM sleep after administration of haloperidol combined with total sleep deprivation in healthy young subjects
Provedor de dados: 56
Autores: Pinto Jr.,L.R.; Peres,C.A.; Russo,R.H.; Remesar-Lopez,A.J.; Tufik,S.
We sought to examine the possible participation of dopaminergic receptors in the phasic events that occur during rapid eye movement (REM) sleep, known as sawtooth waves (STW). These phasic phenomena of REM sleep exhibit a unique morphology and, although they represent a characteristic feature of REM sleep, little is known about the mechanisms which generate them and which are apparently different from rapid eye movements. STW behavior was studied in 10 male volunteers aged 20 to 35 years, who were submitted to polysomnographic monitoring (PSG). On the adaptation night they were submitted to the first PSG and on the second night, to the basal PSG. On the third night the volunteers received placebo or haloperidol and spent the whole night awake. On the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sawtooth waves; REM sleep; Sleep deprivation; Dopaminergic receptors; Rapid eye movements; Haloperidol.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500013

Differential response to gepirone but not to chlordiazepoxide in malnourished rats subjected to learned helplessness
Provedor de dados: 56
Autores: Camargo,L.M.M; Nascimento,A.B; Almeida,S.S.
The learned helplessness (LH) paradigm is characterized by learning deficits resulting from inescapable events. The aims of the present study were to determine if protein-calorie malnutrition (PCM) alters learning deficits induced by LH and if the neurochemical changes induced by malnutrition alter the reactivity to treatment with GABA-ergic and serotonergic drugs during LH. Well-nourished (W) and PCM Wistar rats (61 days old) were exposed or not to inescapable shocks (IS) and treated with gepirone (GEP, 0.0-7.5 mg/kg, intraperitoneally, N = 128) or chlordiazepoxide (0.0-7.5 mg/kg, intraperitoneally, N = 128) 72 h later, 30 min before the test session (30 trials of escape learning). The results showed that rats exposed to IS had higher escape latency than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein-calorie malnutrition; Learned helplessness; Chlordiazepoxide; Gepirone; Rats.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100009

Trabeculectomy and optic nerve head topography
Provedor de dados: 56
Autores: Paranhos Jr.,A.; Lima,M.C.; Salim,S.; Caprioli,J.; Shields,M.B.
The objective of the present study was to evaluate changes in optic nerve head parameters, measured by confocal laser tomography, before and after trabeculectomy in order to identify outcome measures for the management of glaucoma. The optic nerve head of 22 eyes (22 patients) was analyzed by confocal laser tomography with the Heidelberg retinal tomogram (HRT) before and after trabeculectomy. The median time between the first HRT and surgery was 4.6 months (mean: 7.7 ± 8.3) and the median time between surgery and the second HRT was 10.8 months (mean: 12.0 ± 6.8). The patients were divided into two groups, i.e., those with the highest (group A) and lowest (group B) intraocular pressure (IOP) change after surgery. Differences in the 12 standard topographic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Optic nerve head; Confocal laser tomography; Intraocular pressure; Trabeculectomy; Glaucoma.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100017

Vitamin D receptor alleles and bone mineral density in a normal premenopausal Brazilian female population
Provedor de dados: 56
Autores: Lazaretti-Castro,M.; Duarte-de-Oliveira,M.A.; Russo,E.M.K.; Vieira,J.G.H.
Studies on the association between vitamin D receptor (VDR) polymorphism and bone mineral density (BMD) in different populations have produced conflicting results probably due to ethnic differences in the populations studied. The Brazilian population is characterized by a very broad genetic background and a high degree of miscegenation. Of an initial group of 164, we studied 127 women from the city of São Paulo, aged 20 to 47 years (median, 31 years), with normal menses, a normal diet and no history of diseases or use of any medication that could alter BMD. VDR genotype was assessed by PCR amplification followed by BsmI digestion of DNA isolated from peripheral leukocytes. BMD was measured using dual energy X-ray absorptiometry (Lunar DPX) at the lumbar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitamin D receptor genotype; Bone mineral density; Brazilian population.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800003

Isolation and intracellular localization of insulin-like proteins from leaves of Bauhinia variegata
Provedor de dados: 56
Autores: Azevedo,C.R.; Maciel,F.M.; Silva,L.B.; Ferreira,A.T.S.; da Cunha,M.; Machado,O.L.T.; Fernandes,K.V.S.; Oliveira,A.E.A.; Xavier-Filho,J.
Evidence based on immunological cross-reactivity and anti-diabetic properties has suggested the presence of insulin-like peptides in plants. The objective of the present study was to investigate the presence of insulin-like proteins in the leaves of Bauhinia variegata ("pata-de-vaca", "mororó"), a plant widely utilized in popular medicine as an anti-diabetic agent. We show that an insulin-like protein was present in the leaves of this plant. A chloroplast protein with a molecular mass similar to that of bovine insulin was extracted from 2-mm thick 15% SDS-PAGE gels and fractionated with a 2 x 24 cm Sephadex G-50 column. The activity of this insulin-like protein (0.48 mg/mL) on serum glucose levels of four-week-old Swiss albino (CF1) diabetic mice was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bauhinia variegata; Plant insulin; Chloroplast; Medicinal plant.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100007

Use of fluorescent probes to follow membrane traffic in nerve terminals
Provedor de dados: 56
Autores: Guatimosim,C.; Romano-Silva,M.A.; Gomez,M.V.; Prado,M.A.M.
Optical tracers in conjunction with fluorescence microscopy have become widely used to follow the movement of synaptic vesicles in nerve terminals. The present review discusses the use of these optical methods to understand the regulation of exocytosis and endocytosis of synaptic vesicles. The maintenance of neurotransmission depends on the constant recycling of synaptic vesicles and important insights have been gained by visualization of vesicles with the vital dye FM1-43. A number of questions related to the control of recycling of synaptic vesicles by prolonged stimulation and the role of calcium to control membrane internalization are now being addressed. It is expected that optical monitoring of presynaptic activity coupled to appropriate genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laser scanning confocal microscopy; FM1-43; Exocytosis; Synaptic vesicles; Endocytosis.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100018

Central 5-HT2A receptors modulate the vagal bradycardia in response to activation of the von Bezold-Jarisch reflex in anesthetized rats
Provedor de dados: 56
Autores: Futuro Neto,H.A.; Macedo,S.M.; Silva,N.F.; Cabral,A.M.; Pires,J.G.P.
Activation of 5-hydroxytryptamine (5-HT) 5-HT1A, 5-HT2C, 5-HT3, and 5-HT7 receptors modulates the excitability of cardiac vagal motoneurones, but the precise role of 5-HT2A/2B receptors in these phenomena is unclear. We report here the effects of intracisternal (ic) administration of selective 5-HT2A/2B antagonists on the vagal bradycardia elicited by activation of the von Bezold-Jarisch reflex with phenylbiguanide. The experiments were performed on urethane-anesthetized male Wistar rats (250-270 g, N = 7-9 per group). The animals were placed in a stereotaxic frame and their atlanto-occipital membrane was exposed to allow ic injections. The rats received atenolol (1 mg/kg, iv) to block the sympathetic component of the reflex bradycardia; 20-min later, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5-HT2 receptors; R-96544; Reflex bradycardia; SB-204741; Vagal motoneurons; Von Bezold-Jarisch reflex.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300008

Sorafenib prevents liver fibrosis in a non-alcoholic steatohepatitis (NASH) rodent model
Provedor de dados: 56
Autores: Stefano,J.T.; Pereira,I.V.A.; Torres,M.M.; Bida,P.M.; Coelho,A.M.M.; Xerfan,M.P.; Cogliati,B.; Barbeiro,D.F.; Mazo,D.F.C.; Kubrusly,M.S.; D'Albuquerque,L.A.C.; Souza,H.P.; Carrilho,F.J.; Oliveira,C.P.
Liver fibrosis occurring as an outcome of non-alcoholic steatohepatitis (NASH) can precede the development of cirrhosis. We investigated the effects of sorafenib in preventing liver fibrosis in a rodent model of NASH. Adult Sprague-Dawley rats were fed a choline-deficient high-fat diet and exposed to diethylnitrosamine for 6 weeks. The NASH group (n=10) received vehicle and the sorafenib group (n=10) received 2.5 mg·kg-1·day-1 by gavage. A control group (n=4) received only standard diet and vehicle. Following treatment, animals were sacrificed and liver tissue was collected for histologic examination, mRNA isolation, and analysis of mitochondrial function. Genes related to fibrosis (MMP9, TIMP1, TIMP2), oxidative stress (HSP60, HSP90, GST), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NASH; Fibrosis; Mitochondrial dysfunction; Sorafenib.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500408

Perturbation of EGF-induced MAP kinase activation by TGF-ß1
Provedor de dados: 56
Autores: Wenner,C.E.; Yan,S.
TGF-ß1 regulates both cellular growth and phenotypic plasticity important for maintaining a growth advantage and increased invasiveness in progressively malignant cells. Recent studies indicate that TGF-ß-1 stimulates the conversion of epitheliod to fibroblastoid phenotype which presumably leads to the inactivation of growth-inhibitory effects by TGF-ß1 (Portella et al. (1998) Cell Growth and Differentiation, 9: 393-404). Therefore, the investigation of TGF-ß1 signaling that leads to altered growth and migration may provide novel targets for the prevention of increased cell growth and invasion. Although much attention has been paid to TGF-ß1 responses in epithelial cells, the above studies suggest that examination of signal transduction pathways in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transforming growth factor; MAP kinase; Cyclin D1.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700004

Effects of chronic corticosterone and imipramine administration on panic and anxiety-related responses
Provedor de dados: 56
Autores: Diniz,L.; dos Reis,B.B.; de Castro,G.M.; Medalha,C.C.; Viana,M.B.
It is known that chronic high levels of corticosterone (CORT) enhance aversive responses such as avoidance and contextual freezing. In contrast, chronic CORT does not alter defensive behavior induced by the exposure to a predator odor. Since different defense-related responses have been associated with specific anxiety disorders found in clinical settings, the observation that chronic CORT alters some defensive behaviors but not others might be relevant to the understanding of the neurobiology of anxiety. In the present study, we investigated the effects of chronic CORT administration (through surgical implantation of a 21-day release 200 mg pellet) on avoidance acquisition and escape expression by male Wistar rats (200 g in weight at the beginning of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Corticosterone; Imipramine; Generalized anxiety disorder; Panic disorder; Elevated T-maze.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000010

Neo-Timm staining in the thalamus of chronically epileptic rats
Provedor de dados: 56
Autores: Hamani,C.; Paulo,I. de; Mello,L.E.A.M.
The thalamus is an important modulator of seizures and is severely affected in cholinergic models of epilepsy. In the present study, chronically epileptic rats had their brains processed for neo-Timm and acetylcholinesterase two months after the induction of status epilepticus with pilocarpine. Both controls and pilocarpine-treated animals presented neo-Timm staining in the anterodorsal nucleus, laterodorsal nucleus, reticular nucleus, most intralaminar nuclei, nucleus reuniens, and rhomboid nucleus of the thalamus, as well as in the zona incerta. The intensity of neo-Timm staining was similar in control and pilocarpine-treated rats, except for the nucleus reuniens and the rhomboid nucleus, which had a lower intensity of staining in the epileptic group. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epilepsy; Pilocarpine; Zinc; Sprouting; Nucleus reuniens; Midline thalamus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100016

A comparative study of aging of the elastic fiber system of the diaphragm and the rectus abdominis muscles in rats
Provedor de dados: 56
Autores: Rodrigues,C.J.; Rodrigues Junior,A.J.
In the present study the age-related changes of the striated muscle elastic fiber system were investigated in the diaphragm and rectus abdominis muscles of 1-, 4-, 8- and 18-month-old rats. The activation patterns of these muscles differ in that the diaphragm is regularly mobilized tens of times every minute during the entire life of the animal whereas the rectus abdominis, although mobilized in respiration, is much less and more irregularly activated. The elastic fibers were stained by the Verhoeff technique for mature elastic fibers. Weigert stain was used to stain mature and elaunin elastic fibers, and Weigert-oxone to stain mature, elaunin and oxytalan elastic fibers. The density of mature and elaunin elastic fibers showed a progressive increase with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Elastin; Extracellular matrix; Diaphragm; Rectus abdominis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200008

Interleukin-6 promoter polymorphisms (-174 G/C) in Malaysian patients with systemic lupus erythematosus
Provedor de dados: 56
Autores: Chua,K.H.; Kee,B.P.; Tan,S.Y.; Lian,L.H.
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that involves the inflammation of various organs upon deposition of immune complexes and is characterized by uncontrolled B cell hyperactivity. Despite intensive research on the etiology of the disease, the exact cause of the onset of SLE is unknown. The pathogenesis of the disease has been proposed to be associated with the imbalance of T helper type 1 (Th1) and Th2 cytokine activities. Elevated serum levels of interleukin-6 (IL-6), a Th2 cytokine with various functions in the regulation of human biological systems, are observed in SLE patients. In the present study, 100 Malaysian SLE patients and 100 controls were evaluated in order to determine the association of polymorphisms existing...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Interleukin-6; Systemic lupus erythematosus; Promoter; Polymorphisms.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600012

Evidence of the effect of dipyrone on the central nervous system as a determinant of delayed gastric emptying observed in rats after its administration
Provedor de dados: 56
Autores: Collares,E.F.; Vinagre,A.M.
Dipyrone administered intravenously (iv) delays gastric emptying (GE) in rats. The objectives of the present study were to assess: 1) the effect of the dose of dipyrone and time after its iv administration on GE in rats, 2) the effect of subdiaphragmatic vagotomy (VgX) and bilateral electrolytic lesion of the paraventricular nucleus (PVNX) on the delayed GE induced by the drug, and 3) the intracerebroventricular (icv) action of dipyrone and of one of its metabolites, 4-aminoantipyrine on GE. Male Wistar rats received saline labeled with phenol red intragastrically as a test meal. GE was indirectly assessed by the determination of percent gastric retention (GR) of the test meal 10 min after administration by gavage. Dipyrone delays GE in a dose- and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Dipyrone; Paraventricular nucleus; Vagus nerve.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000014

Is there an association between T102C polymorphism of the serotonin receptor 2A gene and urinary incontinence?
Provedor de dados: 56
Autores: Schwanke,C.H.A.; Bittencourt,L.; Noronha,J.A.P.; Augustin,S.A.J.; Jung,I.E.; Cruz,I.B.M.
The regulation of bladder function is influenced by central serotonergic modulation. Several genetic polymorphisms related to serotonin control have been described in the literature. T102C polymorphism of the serotonin receptor 2A gene (5-HT2A) has been shown to be associated with certain diseases such as non-fatal acute myocardial infarction, essential hypertension, and alcoholism. In the present study, we examined the association between 5-HT2A gene polymorphism and urinary incontinence in the elderly. A case-control study was performed in 298 elderly community dwellers enrolled in the Gravataí-GENESIS Project, Brazil, which studies gene-environmental interactions in aging and age-related diseases. Clinical, physical, biochemical, and molecular analyses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: T102C polymorphism of the serotonin receptor 2A gene; 5-HT2A gene polymorphism; Urinary incontinence; Elderly patients.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000003

Depression and diagnosis of neurocognitive impairment in HIV-positive patients
Provedor de dados: 56
Autores: Pinheiro,C.A.T.; Souza,L.D.M.; Motta,J.V.S.; Kelbert,E.F.; Souza,M.S.; Martins,C.S.R.; Coelho,F.M.C.; Pinheiro,K.A.T.; Pinheiro,R.T.
Neurocognitive impairment (NCI) is frequently observed in patients infected with human immunodeficiency virus (HIV) and results from the compromise of subcortical brain structures by the virus. The manifestations of NCI range from asymptomatic impairment to dementia. In addition to cognitive impairment resulting from HIV infection, other factors such as depression are associated with the loss of cognitive functions. The aim of this study was to estimate the prevalence of NCI in HIV-positive patients in a city in southern Brazil and to establish possible associations for the prevalence of NCI with HIV-related and other risk factors. This cross-sectional study of HIV-positive outpatients was conducted in a specialized care service in the city of Pelotas in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AIDS; Depression; Neurocognitive; HIV; HAND; CD4.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000704

Clinical trial of gabexate in the prophylaxis of post-endoscopic retrograde cholangiopancreatography pancreatitis
Provedor de dados: 56
Autores: Xiong,G.-S.; Wu,S.-M.; Zhang,X.-W.; Ge,Z.-Z.
The objective of the present study was to determine the efficacy of prophylactic administration of gabexate for the prevention of post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis, hyperamylasemia and pancreatic pain. Patients scheduled for ERCP were randomized into two groups in a double-blind manner: the patients in the gabexate group were treated with continuous intravenous infusion of 300 mg gabexate dissolved in 500 mL Ringer's solution at 111 mL/h, starting 30 min before the endoscopic maneuvers and continuing up to 4 h after them; placebo group patients were treated only with Ringer's solution also starting 30 min before the endoscopic maneuvers and continuing up to 4 h. Data for 193 patients were analyzed. The incidence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gabexate; Endoscopic retrograde cholangiopancreatography; Post-ERCP pancreatitis; Prevention of pancreatitis; Hyperamylasemia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100010

Comparison of conventional Papanicolaou cytology samples with liquid-based cervical cytology samples from women in Pernambuco, Brazil
Provedor de dados: 56
Autores: Costa,M.O.L.P.; Heráclio,S.A.; Coelho,A.V.C.; Acioly,V.L.; Souza,P.R.E.; Correia,M.T.S.
In the present study, we compared the performance of a ThinPrep cytological method with the conventional Papanicolaou test for diagnosis of cytopathological changes, with regard to unsatisfactory results achieved at the Central Public Health Laboratory of the State of Pernambuco. A population-based, cross-sectional study was performed with women aged 18 to 65 years, who spontaneously sought gynecological services in Public Health Units in the State of Pernambuco, Northeast Brazil, between April and November 2011. All patients in the study were given a standardized questionnaire on sociodemographics, sexual characteristics, reproductive practices, and habits. A total of 525 patients were assessed by the two methods (11.05% were under the age of 25 years,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Papanicolaou cytology; Liquid-based cytology; ThinPrep.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900831

Pharmacokinetics and pharmacodynamics of propranolol in hypertensive patients after sublingual administration: systemic availability
Provedor de dados: 56
Autores: Mansur,A.P.; Avakian,S.D.; Paula,R.S.; Donzella,H.; Santos,S.R.C.J.; Ramires,J.A.F.
The bioavailability of propranolol depends on the degree of liver metabolism. Orally but not intravenously administered propranolol is heavily metabolized. In the present study we assessed the pharmacokinetics and pharmacodynamics of sublingual propranolol. Fourteen severely hypertensive patients (diastolic blood pressure (DBP) &lt;FONT FACE="Symbol"&gt;³&lt;/font&gt;115 mmHg), aged 40 to 66 years, were randomly chosen to receive a single dose of 40 mg propranolol hydrochloride by sublingual or peroral administration. Systolic (SBP) and diastolic (DBP) blood pressures, heart rate (HR) for pharmacodynamics and blood samples for noncompartmental pharmacokinetics were obtained at baseline and at 10, 20, 30, 60 and 120 min after the single dose. Significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propranolol; Pharmacokinetics; Pharmacodynamics; Arterial hypertension; Sublingual vs peroral administration.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500014

Propranolol inhibits the proliferation, migration and tube formation of hemangioma cells through HIF-1α dependent mechanisms
Provedor de dados: 56
Autores: Chen,Y.Z.; Bai,N.; Bi,J.H.; Liu,X.W.; Xu,G.Q.; Zhang,L.F.; Li,X.Q.; Huo,R.
The aim of this study was to investigate the mechanism of propranolol on the regression of hemangiomas. Propranolol-treated hemangioma tissues were collected and the expression of hypoxia inducible factor-1α (HIF-1α) was examined. We also established HIF-1α overexpression and knockdown hemangioma cells, and determined the effects of HIF-1α on the hemangioma cells proliferation, apoptosis, migration and tube formation. Significantly increased HIF-1α level was found in the hemangioma tissues compared to that in normal vascular tissues, whereas propranolol treatment decreased the HIF-1α level in hemangioma tissues in a time- and dose-dependent manner. Moreover, propranolol treatment significantly decreased cell proliferation, migration and tube formation as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propranolol; Hemangiomas; Hypoxia inducible factor-1α; Knockdown; Regression.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200601

Comparison of the specificity of PCR and the histopathological detection of leishmania for the diagnosis of American cutaneous leishmaniasis
Provedor de dados: 56
Autores: Medeiros,A.C.R.; Rodrigues,S.S.; Roselino,A.M.F.
More precise and rapid diagnostic methods for American cutaneous leishmaniasis (ACL) are necessary because of the growing number of cases observed in Brazil, including the northeastern region of the State of São Paulo. We applied PCR to 54 skin or mucosal biopsies from patients with a clinical and/or laboratory diagnosis of ACL using primers 13A and 13B, with positive results being obtained for 82% of the samples. When the PCR results were compared to those of histopathological leishmania detection, PCR showed superior results with 81.5% sensitivity and 95% CI of 68.0-95.1%. The Montenegro skin test (MST) was positive in 88.7% of patients. Since MST cannot be used as a diagnostic tool in endemic areas, the present results strongly suggest the use of PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Cutaneous leishmaniasis; PCR; Histopathological detection; Leishmania detection.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400002

Antithrombotic effect of Lonomia obliqua caterpillar bristle extract on experimental venous thrombosis
Provedor de dados: 56
Autores: Prezoto,B.C.; Maffei,F.H.A.; Mattar,L.; Chudzinski-Tavassi,A.M.; Curi,P.R.
The venom of Lonomia obliqua caterpillar may induce a hemorrhagic syndrome in humans, and blood incoagulability by afibrinogenemia when intravenously injected in laboratory animals. The possible antithrombotic and thrombolytic activities of L. obliqua caterpillar bristle extract (LOCBE) were evaluated in this study. The minimal intravenous dose of the extract necessary to induce afibrinogenemia and anticoagulation was 3.0 and 10.0 µg protein/kg body weight for rabbits and rats, respectively. In rabbits, this dose induced total blood incoagulability for at least 10 h and did not reduce the weight of preformed venous thrombi, in contrast to streptokinase (30,000 IU/kg). In rats, pretreatment with 5.0 and 10.0 µg/kg LOCBE prevented the formation of thrombi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thrombosis; Fibrinolysis; Anticoagulant; Venom; Lonomia obliqua caterpillar.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600011

Acute phenobarbital administration induces hyperalgesia: pharmacological evidence for the involvement of supraspinal GABA-A receptors
Provedor de dados: 56
Autores: Yokoro,C.M.; Pesquero,S.M.S.; Turchetti-Maia,R.M.M.; Francischi,J.N.; Tatsuo,M.A.K.F.
The aim of the present study was to determine if phenobarbital affects the nociception threshold. Systemic (1-20 mg/kg) phenobarbital administration dose dependently induced hyperalgesia in the tail-flick, hot-plate and formalin tests in rats and in the abdominal constriction test in mice. Formalin and abdominal constriction tests were the most sensitive procedures for the detection of hyperalgesia in response to phenobarbital compared with the tail-flick and hot-plate tests. The hyperalgesia induced by systemic phenobarbital was blocked by previous administration of 1 mg/kg ip picrotoxin or either 1-2 mg/kg sc or 10 ng icv bicuculline. Intracerebroventricular phenobarbital administration (5 µg) induced hyperalgesia in the tail-flick test. In contrast,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenobarbital; Hyperalgesia; GABA-A receptors.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300015

Morphological and functional alterations of the intestine of rats with iron-deficiency anemia
Provedor de dados: 56
Autores: Wayhs,M.L.C.; Patrício,F.S.R.; Amancio,O.M.S.; Pedroso,M.Z.; Fagundes Neto,U.; Morais,M.B.
The present study was designed to assess the intestinal absorption of D-xylose and jejunal morphometry in rats with iron-deficiency anemia. Male Wistar rats were randomly divided into a control group (diet containing 50 mg Fe/kg, N = 12) and an anemic group (diet containing &lt;5 mg Fe/kg, N = 12). The animals were housed in individual metabolic cages and deionized water and diet were provided ad libitum for 6 weeks. Hemoglobin and hematocrit were determined at 0, 2, 4, and 6 weeks. At the end of the study the rats were submitted to a D-xylose absorption test (50 mg/100 g body weight) and sacrificed and a jejunal specimen was obtained for morphometric study. At the end of the study the hemoglobin and hematocrit of the anemic rats (8.7 ± 0.9 g/dl and 34.1 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iron deficiency; Anemia; Jejunal villus height; Jejunal mucosal thickness; Jejunal epithelial surface.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100006

Molecular analysis of CIB4 gene and protein in Kermani sheep
Provedor de dados: 56
Autores: Mohammadabadi,M.R.; Jafari,A.H.D.; Bordbar,F.
The human calcium- and integrin-binding protein (CIB) family is composed of CIB1, CIB2, CIB3, and CIB4 proteins and the CIB4 gene affects fertility. Kermani sheep is one of the most important breeds of Iranian sheep breeds. The aim of this study was to analyze for the first time molecular characteristics of the CIB4 gene and protein in Kermani sheep. Different tissues were collected from the Kermani sheep and real time PCR was performed. The PCR products were sequenced, comparative analyses of the nucleotide sequences were performed, a phylogenetic tree was constructed, and different characteristics of CIB4 proteins were predicted. Real time PCR results showed that the CIB4 gene is expressed only in testis of Kermani sheep. The cDNA nucleotide sequence was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CIB4 gene; Kermani sheep; Molecular characteristics; Testis; Interaction.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100602

Complications of surgical treatment of cervical carcinoma
Provedor de dados: 56
A total of 302 patients with stage Ib and IIa cervical carcinoma were submitted to radical hysterectomy and lymphadenectomy during the period from 1980 to 1994. The morbidity rate was 37.5% and the mortality rate 0.6%. The most common intraoperative complications were injuries to the great pelvic vessels and the most frequent postoperative complications involved the urinary tract. The leading causes of morbidity were urinary infection (20.8%), bladder dysfunction (9.2%) and ureteral fistulas (2.9%). Although the rate of complications was high, morbidity has been decreasing over the last five years. Thus, radical hysterectomy continues to be one of the methods for the treatment of early cervical carcinoma that presents an acceptable 5-year survival rate.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Early cervical carcinoma; Radical hysterectomy; Surgical complications.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100005

Identification of the divergent calmodulin binding motif in yeast Ssb1/Hsp75 protein and in other HSP70 family members
Provedor de dados: 56
Autores: Heinen,R.C.; Diniz-Mendes,L.; Silva,J.T.; Paschoalin,V.M.F.
Yeast soluble proteins were fractionated by calmodulin-agarose affinity chromatography and the Ca2+/calmodulin-binding proteins were analyzed by SDS-PAGE. One prominent protein of 66 kDa was excised from the gel, digested with trypsin and the masses of the resultant fragments were determined by MALDI/MS. Twenty-one of 38 monoisotopic peptide masses obtained after tryptic digestion were matched to the heat shock protein Ssb1/Hsp75, covering 37% of its sequence. Computational analysis of the primary structure of Ssb1/Hsp75 identified a unique potential amphipathic alpha-helix in its N-terminal ATPase domain with features of target regions for Ca2+/calmodulin binding. This region, which shares 89% similarity to the experimentally determined calmodulin-binding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ssb1/Hsp75; Ca2+/calmodulin complex; Hsc70; Calmodulin-affinity peptide; Bag1; Saccharomyces cerevisiae.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100003

Serum and cerebrospinal fluid concentrations of E-selectin in patients with aneurysmal subarachnoid hemorrhage
Provedor de dados: 56
Autores: Tanriverdi,T.; Sanus,G.Z.; Ulu,M.O.; Tureci,E.; Uzun,H.; Aydin,S.; Kaynar,M.Y.
The goal of the present study was to determine concentrations of E-selectin in both cerebrospinal fluid (CSF) and serum of patients with aneurysmal subarachnoid hemorrhage (SAH) and to evaluate the correlation between the clinical parameters and E-selectin levels. Both CSF and serum samples obtained from 12 patients with aneurysmal SAH and 8 patients with hydrocephalus (control group) without any other known central nervous system disease were assayed for E-selectin by quantitative enzyme-linked immunosorbent assay and the results were compared between the two groups. Mean levels of soluble forms of E-selectin within the first 3 days and on the 5th and 7th days of SAH were 4.0 ± 7.9, 2.8 ± 5.2, and 3.1 ± 4.9 ng/ml in the patient's CSF, and 33.7 ± 9.2, 35.1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion molecules; E-selectin; Selectin; Subarachnoid hemorrhage; Vasospasm.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100020

The quality control of glycoprotein folding in the endoplasmic reticulum, a trip from trypanosomes to mammals
Provedor de dados: 56
Autores: Parodi,A.J.
The present review deals with the stages of synthesis and processing of asparagine-linked oligosaccharides occurring in the lumen of the endoplasmic reticulum and their relationship to the acquisition by glycoproteins of their proper tertiary structures. Special emphasis is placed on reactions taking place in trypanosomatid protozoa since their study has allowed the detection of the transient glucosylation of glycoproteins catalyzed by UDP-Glc:glycoprotein glucosyltransferase and glucosidase II. The former enzyme has the unique property of covalently tagging improperly folded conformations by catalyzing the formation of protein-linked Glc1Man7GlcNAc2, Glc1Man8GlcNac2 and Glc1Man9GlcNAc2 from the unglucosylated proteins. Glucosyltransferase is a soluble...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycoproteins; N-glycosylation; Folding; Endoplasmic reticulum; Glucosylation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500002

Topographic aspects of photic driving in the electroencephalogram of children and adolescents
Provedor de dados: 56
Autores: Lazarev,V.V.; Infantosi,A.F.C.; Valencio-de-Campos,D.; Azevedo,L.C. de.
The electroencephalogram amplitude spectra at 11 fixed frequencies of intermittent photic stimulation of 3 to 24 Hz were combined into driving "profiles" for 14 scalp points in 8 male and 7 female normal subjects aged 9 to 17 years. The driving response varied over frequency and was detected in 70 to 100% of cases in the occipital areas (maximum) and in 27 to 77% of cases in the frontal areas (minimum) using as a criterion peak amplitude 20% higher than those of the neighbors. Each subject responded, on average, to 9.7 ± 1.15 intermittent photic stimulation frequencies in the right occipital area and to 6.8 ± 1.97 frequencies in the right frontal area. Most of the driving responses (in relation to the previous background) were significant according to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electroencephalogram; Intermittent photic stimulation; Photic driving; Brain topography; Children; Adolescents.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600014

Bone mineral density in young women of the city of São Paulo, Brazil: correlation with both collagen type I alpha 1 gene polymorphism and clinical aspects
Provedor de dados: 56
Autores: Barros,E.R.; Kasamatsu,T.S.; Ramalho,A.C.; Hauache,O.M.; Vieira,J.G.H.; Lazaretti-Castro,M.
Osteoporosis is a multifactorial disease with great impact on morbidity and mortality mainly in postmenopausal women. Although it is recognized that factors related to life-style and habits may influence bone mass formation leading to greater or lower bone mass, more than 85% of the variation in bone mineral density (BMD) is genetically determined. The collagen type I alpha 1 (COLIA1) gene is a possible risk factor for osteoporosis. We studied a population of 220 young women from the city of São Paulo, Brazil, with respect to BMD and its correlation with both COLIA1 genotype and clinical aspects. The distribution of COLIA1 genotype SS, Ss and ss in the population studied was 73.6, 24.1 and 2.3%, respectively. No association between these genotypes and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COLIA1; Bone mineral density; Risk factors; Polymorphism; Osteoporosis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800005

Effect of fasudil on experimental autoimmune neuritis and its mechanisms of action
Provedor de dados: 56
Autores: Zhao,Yanyin; Liu,Bingyou; Wang,Yi; Xiao,Baoguo.
This study aimed to investigate the therapeutic effect of fasudil on treating experimental autoimmune neuritis (EAN). Twenty-four EAN mice were randomly assigned to fasudil treatment (Fasudil group) or saline treatment (EAN model group) for 28 days. Clinical symptom score was evaluated every other day; inflammatory cell infiltration, demyelination, anti-myelin basic protein (MBP), inflammatory cytokines, inducible nitric oxide synthase (iNOS), and arginase-1 were detected in sciatic nerves at day 28. Th1, Th2, Th17, and Tregs proportions in splenocytes were detected at day 28. Clinical symptom score was found to be attenuated in the Fasudil group compared to the EAN model group from day 12 to day 28. Sciatic nerve inflammatory cell counts by HE staining...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fasudil; Experimental autoimmune neuritis; Th1; Th17; Tregs; M2 macrophages polarization.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100607

Absorption mechanism of three curcumin constituents through in situ intestinal perfusion method
Provedor de dados: 56
Autores: Wang,Y.-H.; Ke,X.-M.; Zhang,C.-H.; Yang,R.-P.
This study aimed to investigate the absorption mechanism of three curcumin constituents in rat small intestines. Self-emulsification was used to solubilize the three curcumin constituents, and the rat in situ intestinal perfusion method was used to study factors on drug absorption, including drug mass concentration, absorption site, and the different types and concentrations of absorption inhibitors. Within the scope of experimental concentrations, three curcumin constituents were absorbed in rat small intestines through the active transport mechanism.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcumin; Demethoxycurcumin; Bisdethoxycurcumin; In situ intestinal perfusion; Absorption mechanism.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100612

Anti-inflammatory and antinociceptive activities of an acid fraction of the seeds of Carpotroche brasiliensis (Raddi) (Flacourtiaceae)
Provedor de dados: 56
Autores: Lima,J.A.; Oliveira,A.S.; Miranda,A.L.P. de; Rezende,C.M.; Pinto,A.C.
Carpotroche brasiliensis is a native Brazilian tree belonging to the Oncobeae tribe of Flacourtiaceae. The oil extracted from its seeds contains as major constituents the same cyclopentenyl fatty acids hydnocarpic (40.5%), chaulmoogric (14.0%) and gorlic (16.1%) acids found in the better known chaulmoogra oil prepared from the seeds of various species of Hydnocarpus (Flacourtiaceae). These acids are known to be related to the pharmacological activities of these plants and to their use as anti-leprotic agents. Although C. brasiliensis oil has been used in the treatment of leprosy, a disease that elicits inflammatory responses, the anti-inflammatory and analgesic activities of the oil and its constituents have never been characterized. We describe the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carpotroche brasiliensis; Flacourtiaceae; Cyclopentenyl fatty acids; Anti-inflammatory effec; Analgesic; Antinociceptive effect.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700013

DNA encoding individual mycobacterial antigens protects mice against tuberculosis
Provedor de dados: 56
Autores: Silva,C.L.; Bonato,V.L.D.; Lima,V.M.F.
Over the last few years, some of our experiments in which mycobacterial antigens were presented to the immune system as if they were viral antigens have had a significant impact on our understanding of protective immunity against tuberculosis. They have also markedly enhanced the prospects for new vaccines. We now know that individual mycobacterial protein antigens can confer protection equal to that from live BCG vaccine in mice. A critical determinant of the outcome of immunization appears to be the degree to which antigen-specific cytotoxic T cells are generated by the immune response. Our most recent studies indicate that DNA vaccination is an effective way to establish long-lasting cytotoxic T cell memory and protection against tuberculosis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; DNA vaccination; Cytokines; Th1/Th2.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200012

A prospective study of hepatitis B virus markers in patients with chronic HBV infection from Brazilian families of Western and Asian origin
Provedor de dados: 56
Autores: Carrilho,F.J.; Ono-Nita,S.K.; Cardoso,R.A.; Cancado,E.L.R.; Pinho,J.R.R.; Alves,V.A.F.; Da Silva,L.C.
The purpose of the present study was to determine the frequency of hepatitis B virus (HBV) markers in families of HBsAg-positive patients with chronic liver disease. Serum anti-HBc, HBsAg and anti-HBs were determined by enzyme immunoassay and four subpopulations were considered: genetically related (consanguineous) and non-genetically related (non-consanguineous) Asian subjects and genetically related and non-genetically related Western subjects. A total of 165 and 186 relatives of Asian and Western origin were enrolled, respectively. The occurrence of HBsAg and anti-HBs antibodies was significantly higher (P &lt; 0.0001) in family members of Asian origin (81.8%) than in family members of Western origin (36.5%). HBsAg was also more frequent among brothers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B; Familial clustering; HBsAg; Vertical transmission; Anti-HBc; Anti-HBs.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900015

Successful domino liver transplantation in maple syrup urine disease using a related living donor
Provedor de dados: 56
Autores: Feier,F.H.; Miura,I.K.; Fonseca,E.A.; Porta,G.; Pugliese,R.; Porta,A.; Schwartz,I.V.D.; Margutti,A.V.B.; Camelo Jr,J.S.; Yamaguchi,S.N.; Taveira,A.T.; Candido,H.; Benavides,M.; Danesi,V.; Guimaraes,T.; Kondo,M.; Chapchap,P.; Neto,J. Seda.
Maple syrup urine disease (MSUD) is an autosomal recessive disease associated with high levels of branched-chain amino acids. Children with MSUD can present severe neurological damage, but liver transplantation (LT) allows the patient to resume a normal diet and avoid further neurological damage. The use of living related donors has been controversial because parents are obligatory heterozygotes. We report a case of a 2-year-old child with MSUD who underwent a living donor LT. The donor was the patient's mother, and his liver was then used as a domino graft. The postoperative course was uneventful in all three subjects. DNA analysis performed after the transplantation (sequencing of the coding regions of BCKDHA, BCKDHB, andDBT genes) showed that the MSUD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heterozygous donor; Metabolic disease; Branched-chain ketoacid dehydrogenase mutation; Leucine; Genotype.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600522

Effect of all-trans retinoic acid on newly diagnosed acute promyelocytic leukemia patients: results of a Brazilian center
Provedor de dados: 56
Autores: de-Medeiros,B.C.; Strapasson,E.; Pasquini,R.; de-Medeiros,C.R.
Thirty-seven patients with acute promyelocytic leukemia (APL) were treated with all-trans retinoic acid (ATRA). Patients received 45 mg m-2 day-1 po of ATRA until complete remission (CR) was achieved, defined as: a) presence of less than 5% blasts in the bone marrow, with b) white blood cells &gt;103/mm3, c) platelets &gt;105/mm3 and d) hemoglobin concentration &gt;8 g/dl, with no blood or platelet transfusions. Thirty-one (83.7%) patients achieved CR by day 50, and 75% of these before day 30. Correction of the coagulopathy, achieved between days 2 and 10 (mean, 3 days), was the first evidence of response to treatment. Only one patient had been previously treated with chemotherapy and three had the microgranular variant M3 form. Dryness of skin and mucosae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: All-trans retinoic acid; Acute promyelocytic leukemia; Retinoid acid syndrome.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200005

Natural preference of zebrafish (Danio rerio) for a dark environment
Provedor de dados: 56
Autores: Serra,E.L.; Medalha,C.C.; Mattioli,R.
The zebrafish (Danio rerio) has been used as a model in neuroscience but knowledge about its behavior is limited. The aim of this study was to determine the preference of this fish species for a dark or light environment. Initially we used a place preference test and in a second experiment we applied an exit latency test. A two-chamber aquarium was used for the preference test. The aquarium consisted of a black chamber and a white chamber. In the first experiment the animal was placed in the aquarium and the time spent in the two compartments was recorded for 10 min. More time was spent in the black compartment (Wilcoxon matched-pairs signed-rank test, T = 7, N1 = N2 = 18, P = 0.0001). In the second experiment the animal was placed in the black or white...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Zebrafish Danio rerio behavior preference dark; Light.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200016

Culture of human intestinal epithelial cell using the dissociating enzyme thermolysin and endothelin-3
Provedor de dados: 56
Autores: Liu,Z.; Zhang,P.; Zhou,Y.; Qin,H.; Shen,T.
Epithelium, a highly dynamic system, plays a key role in the homeostasis of the intestine. However, thus far a human intestinal epithelial cell line has not been established in many countries. Fetal tissue was selected to generate viable cell cultures for its sterile condition, effective generation, and differentiated character. The purpose of the present study was to culture human intestinal epithelial cells by a relatively simple method. Thermolysin was added to improve the yield of epithelial cells, while endothelin-3 was added to stimulate their growth. By adding endothelin-3, the achievement ratio (viable cell cultures/total cultures) was enhanced to 60% of a total of 10 cultures (initiated from 8 distinct fetal small intestines), allowing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human intestinal epithelial cell; Cell culture; Thermolysin; Endothelin-3; Cytokeratin; Brush border enzyme.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500006

Activation of a P2Y4-like purinoceptor triggers an increase in cytosolic [Ca2+] in the red blood cells of the lizard Ameiva ameiva (Squamata, Teiidae)
Provedor de dados: 56
Autores: Sartorello,R.; Garcia,C.R.S.
An increasing number of pathophysiological roles for purinoceptors are emerging, some of which have therapeutic potential. Erythrocytes are an important source of purines, which can be released under physiological and physiopathological conditions, acting on purinergic receptors associated with the same cell or with neighboring cells. Few studies have been conducted on lizards, and have been limited to ATP agonist itself. We have previously shown that the red blood cells (RBCs) of the lizard Ameiva ameiva store Ca2+ in the endoplasmic reticulum (ER) and that the purinergic agonist ATP triggers a rapid and transient increase of [Ca2+]c by mobilization of the cation from internal stores. We also reported the ability of the second messenger IP3 to discharge...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Red blood cells; Calcium; Teiidae; Ameiva ameiva; Purinoceptor; UTP.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100002

Delay in gallbladder emptying during the perimenopausal period
Provedor de dados: 56
Autores: Petroianu,A.; Oliveira,L.C.; Vieira,M.I.
A pilot study has ascribed an important role in gallbladder motility and emptying to the perimenopausal period. To assess the effect of this period on gallbladder emptying and cholelithogenesis, 25 women in the perimenopausal period without gallbladder disease were submitted to cholangiography and two ultrasound exams. The time for gallbladder emptying and the presence of cholelithiasis were assessed. All patients presented a delay in gallbladder emptying with no relationship to the pre- or postmenopausal period. This finding was not related to lithogenesis. Gallbladder emptying time is longer during the perimenopausal period.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gallbladder emptying; Menopause; Cholelithiasis; Ultrasound; Cholangiography.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900007

Mechanism of the transforming growth factor-β induction of fibronectin expression in hepatic stem-like cells
Provedor de dados: 56
Autores: Cui,W.; Jin,H.B.; Li,Z.W.
Transforming growth factor-β1 (TGF-β1) plays an important role in the fibrogenic process in the liver. The aim of the present study was to explore the action of TGF-β1 on fibronectin expression in rat hepatic stem-like cells and the underlying mechanisms. The level of fibronectin expression was determined in hepatic stem-like cells (WB cells) before and after TGF-β1 stimulation by RT-PCR and Western blot methods. Using immunogold transmission electron microscopy and the Western blot method, we observed the result of the expression and the distribution of cAMP, phosphorylated Smad3 and Smad7 before and after TGF-β1 treatment. The levels of fibronectin expression in both mRNA and protein increased 4- to 5-fold after TGF-β1 stimulation, reaching an optimum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TGF-β; Fibronectin; Fibrogenesis; Hepatic stem cell; CAMP; Smad.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100006

Anthropometric midarm measurements can detect systemic fat-free mass depletion in patients with chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Sanchez,F.F.; Faganello,M.M.; Tanni,S.E.; Lucheta,P.A.; Pelegrino,N.G.; Hasegawa,S.H.; Ribeiro,S.M.; Godoy,I.
Our objective was to determine whether anthropometric measurements of the midarm (MA) could identify subjects with whole body fat-free mass (FFM) depletion. Fifty-five patients (31% females; age: 64.6 ± 9.3 years) with mild/very severe chronic obstructive pulmonary disease (COPD), 18 smokers without COPD (39% females; age: 49.0 ± 7.3 years) and 23 never smoked controls (57% females; age: 48.2 ± 9.6 years) were evaluated. Spirometry, muscle strength and MA circumference were measured. MA muscle area was estimated by anthropometry and MA cross-sectional area by computerized tomography (CT) scan. Bioelectrical impedance was used as the reference method for FFM. MA circumference and MA muscle area correlated with FFM and biceps and triceps strength. Receiver...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fat-free mass depletion; Midarm anthropometry; Chronic obstructive pulmonary disease; Malnutrition; Nutritional status assessment.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500011

The effects of positive end-expiratory pressure on respiratory system mechanics and hemodynamics in postoperative cardiac surgery patients
Provedor de dados: 56
Autores: Auler Jr.,J.O.C.; Carmona,M.J.C.; Barbas,C.V.; Saldiva,P.H.N.; Malbouisson,L.M.S.
We prospectively evaluated the effects of positive end-expiratory pressure (PEEP) on the respiratory mechanical properties and hemodynamics of 10 postoperative adult cardiac patients undergoing mechanical ventilation while still anesthetized and paralyzed. The respiratory mechanics was evaluated by the inflation inspiratory occlusion method and hemodynamics by conventional methods. Each patient was randomized to a different level of PEEP (5, 10 and 15 cmH2O), while zero end-expiratory pressure (ZEEP) was established as control. PEEP of 15-min duration was applied at 20-min intervals. The frequency dependence of resistance and the viscoelastic properties and elastance of the respiratory system were evaluated together with hemodynamic and respiratory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Postoperative cardiac surgery; PEEP; Respiratory mechanics; Hemodynamics.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100005

Low levels of sex hormone-binding globulin and hyperproinsulinemia as markers of increased pancreatic ß-cell demand in men
Provedor de dados: 56
Autores: Reis,A.F.; Miranda,W.L.; Chacra,A.R.
Low levels of sex hormone-binding globulin (SHBG) are considered to be an indirect index of hyperinsulinemia, predicting the later onset of diabetes mellitus type 2. In the insulin resistance state and in the presence of an increased pancreatic ß-cell demand (e.g. obesity) both absolute and relative increases in proinsulin secretion occur. In the present study we investigated the correlation between SHBG and pancreatic ß-cell secretion in men with different body compositions. Eighteen young men (30.0 ± 2.4 years) with normal glucose tolerance and body mass indexes (BMI) ranging from 22.6 to 43.2 kg/m2 were submitted to an oral glucose tolerance test (75 g) and baseline and 120-min blood samples were used to determine insulin, proinsulin and C-peptide by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Proinsulin; Sex hormone-binding globulin; Obesity.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200006

Human sepsis-associated Escherichia coli (SEPEC) is able to adhere to and invade kidney epithelial cells in culture
Provedor de dados: 56
Autores: Conceição,R.A.; Ludovico,M.S.; Andrade,C.G.T.J.; Yano,T.
The adhesins of extraintestinal pathogenic Escherichia coli are essential for mediating direct interactions between the microbes and the host cell surfaces that they infect. Using fluorescence microscopy and gentamycin protection assays, we observed that 49 sepsis-associated E. coli (SEPEC) strains isolated from human adults adhered to and invaded Vero cells in the presence of D-mannose (100%). In addition, bacteria concentrations of approximately 2 x 10(7) CFU/mL were recovered from Vero cells following an invasion assay. Furthermore, PCR analysis of adhesin genes showed that 98.0% of these SEPEC strains tested positive for fimH, 69.4% for flu, 53.1% for csgA, 38.8% for mat, and 32.7% for iha. Analysis of the invasin genes showed that 16.3% of the SEPEC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; Invasiveness; Escherichia coli; Sepsis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500007

Tea polyphenols induce S phase arrest and apoptosis in gallbladder cancer cells
Provedor de dados: 56
Autores: Wang,Jiaqi; Pan,Yixuan; Hu,Jiacheng; Ma,Qiang; Xu,Yi; Zhang,Yijian; Zhang,Fei; Liu,Yingbin.
Gallbladder cancer (GBC) is the most common malignancy in the biliary tract. Without effective treatment, its prognosis is notoriously poor. Tea polyphenols (TPs) have many pharmacological and health benefits, including antioxidant, anti-inflammatory, anti-tumor, anti-thrombotic, antibacterial, and vasodilatory properties. However, the anti-cancer effect of TPs in human gallbladder cancer has not yet been determined. Cell viability and colony formation assay were used to investigate the cell growth. Cell cycle and apoptosis were evaluated by flow cytometry analysis. Western blot assay was used to detect the expression of proteins related to cell cycle and apoptosis. Human tumor xenografts were used to examine the effect of TPs on gallbladder cancer cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallbladder cancer; Tea polyphenols; Apoptosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400614

Does caffeine ingestion before a short-term sprint interval training promote body fat loss?
Provedor de dados: 56
Autores: Ferreira,G.A.; Felippe,L.C.; Bertuzzi,R.; Bishop,D.J.; Ramos,I.S.; De-Oliveira,F.R.; Lima-Silva,A.E.
We investigated the effect of caffeine ingestion combined with a 2-wk sprint interval training (SIT) on training-induced reductions in body adiposity. Twenty physically-active men ingested either 5 mg/kg of cellulose as a placebo (PLA, n=10) or 5 mg/kg of caffeine (CAF, n=10) 60 min before each SIT session (13×30 s sprint/15 s of rest). Body mass and skinfold thickness were measured pre- and post-training. Energy expenditure was measured at rest, during exercise, and 45 min after exercise in the first SIT session. Body fat was similar between PLA and CAF groups at pre-training (P&gt;0.05). However, there was a significant decrease in body fat after training in the CAF group (−5.9±4.2%, P&lt;0.05) but not in PLA (1.5±8.0%, P&gt;0.05). There was no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High-intensity interval training Body composition Body fat distribution Health 1; 3; 7-Trimethylxanthine.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200609

Influence of intrauterine and extrauterine growth on neurodevelopmental outcome of monozygotic twins
Provedor de dados: 56
Autores: Reolon,R.K.; Rotta,N.T.; Agranonik,M.; Silva,A.A. Moura da; Goldani,M.Z.
There have been indications that intrauterine and early extrauterine growth can influence childhood mental and motor function. The objective of the present study was to evaluate the influence of intrauterine growth restriction and early extrauterine head growth on the neurodevelopmental outcome of monozygotic twins. Thirty-six monozygous twin pairs were evaluated at the corrected age of 12 to 42 months. Intrauterine growth restriction was quantified using the fetal growth ratio. The effects of birth weight ratio, head circumference at birth and current head circumference on mental and motor outcomes were estimated using mixed-effect linear regression models. Separate estimates of the between (interpair) and within (intrapair) effects of each measure on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intrauterine growth restriction; Neurodevelopment; Monozygotic twins; Fetal growth ratio; Head circumference; Neurocognitive development.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800010

Glycoprotein nonmetastatic melanoma protein B accelerates tumorigenesis of cervical cancer in vitro by regulating the Wnt/β-catenin pathway
Provedor de dados: 56
Autores: Xu,Shuxiang; Fan,Yingying; Li,Dongping; Liu,Yan; Chen,Xu.
Cervical cancer is one of the most common cancers among women around the world. However, the underlying mechanism involved in cervical cancer progression is incompletely known. In the present study, we determined the role of glycoprotein nonmetastatic melanoma protein B (GPNMB) in tumorigenesis of cervical cancer. According to the GEO database, we found that GPNMB expression was significantly higher in cervical cancer than in normal cervix epithelium. A similar pattern was observed in GPNMB expression in cultured cervical cancer cells and normal cervical epithelial cells. Compared with the control, GPNMB knockdown significantly decreased the proliferation and migration capacity, but enhanced the apoptosis capacity of SiHa and HeLa cells. Additionally, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical cancer; GPNMB; Tumorigenesis; MMPs; Wnt/β-catenin.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100607

Genetic alterations in head and neck squamous cell carcinomas
Provedor de dados: 56
Autores: Nagai,M.A.
The genetic alterations observed in head and neck cancer are mainly due to oncogene activation (gain of function mutations) and tumor suppressor gene inactivation (loss of function mutations), leading to deregulation of cell proliferation and death. These genetic alterations include gene amplification and overexpression of oncogenes such as myc, erbB-2, EGFR and cyclinD1 and mutations, deletions and hypermethylation leading to p16 and TP53 tumor suppressor gene inactivation. In addition, loss of heterozygosity in several chromosomal regions is frequently observed, suggesting that other tumor suppressor genes not yet identified could be involved in the tumorigenic process of head and neck cancers. The exact temporal sequence of the genetic alterations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic alterations; Oncogene; Tumor suppressor gene; Head and neck cancer.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700015

Effect of pre-exercise carbohydrate availability on fat oxidation and energy expenditure after a high-intensity exercise
Provedor de dados: 56
Autores: Ferreira,G.A.; Felippe,L.C.; Silva,R.L.S.; Bertuzzi,R.; De Oliveira,F.R.; Pires,F.O.; Lima-Silva,A.E.
The aim of this study was to test the hypothesis that reduced pre-exercise carbohydrate (CHO) availability potentiates fat oxidation after an exhaustive high-intensity exercise bout. Eight physically active men underwent a high-intensity exercise (∼95% V̇O2max) until exhaustion under low or high pre-exercise CHO availability. The protocol to manipulate pre-exercise CHO availability consisted of a 90-min cycling bout at ∼70% V̇O2max + 6 × 1-min at 125% V̇O2max with 1-min rest, followed by 48 h under a low- (10% CHO, low-CHO availability) or high-CHO diet (80% CHO, high-CHO availability). Time to exhaustion was shorter and energy expenditure (EE) lower during the high-intensity exercise in low- compared to high-CHO availability (8.6±0.8 and 11.4±1.6 min, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Excess post-exercise oxygen consumption; Fat oxidation; Carbohydrate oxidation; Diet manipulation; Aerobic metabolism.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500617

Correlations between forced oscillation technique parameters and pulmonary densitovolumetry values in patients with acromegaly
Provedor de dados: 56
Autores: Camilo,G.B.; Carvalho,A.R.S.; Machado,D.C.; Mogami,R.; Kasuki,L.; Gadelha,M.R.; Melo,P.L.; Lopes,A.J.
The aims of this study were to evaluate the forced oscillation technique (FOT) and pulmonary densitovolumetry in acromegalic patients and to examine the correlations between these findings. In this cross-sectional study, 29 non-smoking acromegalic patients and 17 paired controls were subjected to the FOT and quantification of lung volume using multidetector computed tomography (Q-MDCT). Compared with the controls, the acromegalic patients had a higher value for resonance frequency [15.3 (10.9-19.7) vs 11.4 (9.05-17.6) Hz, P=0.023] and a lower value for mean reactance [0.32 (0.21-0.64) vs 0.49 (0.34-0.96) cm H2O/L/s2, P=0.005]. In inspiratory Q-MDCT, the acromegalic patients had higher percentages of total lung volume (TLV) for nonaerated and poorly aerated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acromegaly; Tomography; Respiratory function tests; Forced oscillation technique.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000003

DNA vaccines for viral diseases
Provedor de dados: 56
Autores: Donnelly,J.J.; Ulmer,J.B.
DNA plasmids encoding foreign proteins may be used as immunogens by direct intramuscular injection alone, or with various adjuvants and excipients, or by delivery of DNA-coated gold particles to the epidermis through biolistic immunization. Antibody, helper T lymphocyte, and cytotoxic T lymphocyte (CTL) responses have been induced in laboratory and domesticated animals by these methods. In a number of animal models, immune responses induced by DNA vaccination have been shown to be protective against challenge with various infectious agents. Immunization by injection of plasmids encoding foreign proteins has been used successfully as a research tool. This review summarizes the types of DNA vaccine vectors in common use, the immune responses and protective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plasmid; Nucleic acid; Protection; Antibodies; T cells; CTL.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200010

Functional mapping of the cardiorespiratory effects of dorsal and median raphe nuclei in the rat
Provedor de dados: 56
Autores: Alvarenga,R.M.; Pires,J.G.P.; Futuro Neto,H.A.
The dorsal (DRN) and median (MRN) raphe nuclei are important sources of serotonergic innervation to the forebrain, projecting to sites involved in cardiovascular regulation. These nuclei have been mapped using electrical stimulation, which has the limitation of stimulating fibers of passage. The present study maps these areas with chemical stimulation, investigating their influence on cardiorespiratory parameters. Urethane-anesthetized (1.2 g/kg, iv) male Wistar rats (280-300 g) were instrumented for pulsatile and mean blood pressure (MBP), heart rate, renal nerve activity, and respiratory frequency recordings. Microinjections of L-glutamate (0.18 M, 50-100 nl with 1% Pontamine Sky Blue) were performed within the DRN or the MRN with glass micropipettes. At...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dorsal raphe nucleus; Median raphe nucleus; L-glutamate; Blood pressure; Respiration; Sympathetic nerve activity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100022

Effect of SX-3228, a selective ligand for the BZ1 receptor, on sleep and waking during the light-dark cycle in the rat
Provedor de dados: 56
Autores: Alvariño,F.; Monti,J.M.; Jantos,H.; Monti,D.
The effects of the benzodiazepine1 (BZ1) receptor agonist SX-3228 were studied in rats (N = 12) implanted for chronic sleep procedures. Administration of 0.5, 1.0 and 2.5 mg/kg SX-3228, sc, to rats 1 h after the beginning of the light phase of the light-dark cycle induced a significant reduction of rapid-eye-movement sleep (REMS) during the third recording hour. Moreover, slow wave sleep (SWS) was increased during the fourth recording hour after the two largest doses of the compound. Administration of 0.5, 1.0 and 2.5 mg/kg SX-3228 one hour after the beginning of the dark period of the light-dark cycle caused a significant and maintained (6-h recording period) reduction of waking (W), whereas SWS and light sleep (LS) were increased. REMS values tended to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SX-3228; Benzodiazepine1 receptor; Sleep; Waking; REM sleep; Zolpidem; Light-dark cycle.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800011

Feeding manipulation elicits different proliferative responses in the gastrointestinal tract of suckling and weanling rats
Provedor de dados: 56
Autores: Palanch,A.C.; Alvares,E.P.
Food deprivation has been found to stimulate cell proliferation in the gastric mucosa of suckling rats, whereas the weanling period has been reported to be unresponsive in terms of proliferative activity. In the present study we analyze regional differences in the effect of milk or food deprivation on cell proliferation of the epithelia of the esophagus and of five segments of small intestine in suckling, weanling and newly weaned Wistar rats of both sexes. DNA synthesis was determined using tritiated thymidine to obtain labeling indices (LI); crypt depth and villus height were also determined. Milk deprivation decreased LI by 50% in the esophagus (from 15 to 8.35%) and small intestine (from 40 to 20%) of 14-day-old rats. In 18-day-old rats, milk and food...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Developing rats; Milk deprivation; Cell proliferation; Crypt and villus size; Esophagus; Small intestine.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400015

Tissue fusion technology versus suture and staple in porcine bowel anastomosis: an in vivo study
Provedor de dados: 56
Autores: Pan,Hong; Leung,Kevin K.C.; Ng,Enders Kwok Wai.
The aim of this study was to make a comparison between the tissue fusion technique and conventional methods for sealing bowel anastomosis. Eighteen female domestic pigs (Suidae, Sus) were used in our study. Tissue-fused anastomoses (LigaSure groups) were made in 13 animals (5 anastomoses per animal), which were subdivided into 4 groups according to different manufacturing settings: “LigaSure-L-1” and “LigaSure-L-2”, with low energy output level with 1 or 2 device-activated tissue sealing times, and “LigaSure-M” and “LigaSure-H”, with medium or high energy output level. As controls, automatically stapled (GIA group) and hand-sewn (suture group) anastomoses were utilized in 3 and 2 animals, respectively. There was no statistical difference in the overall...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bowel anastomosis; Tissue fusion technology; Porcine; In vivo; Suture; Staple.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500605

Depressed nNOS expression during spine transition in the developing hippocampus of FMR1 KO mice
Provedor de dados: 56
Autores: Xu,Qin; Zhu,Zhiwei; Xu,Jialu; Gu,Weizhong; Zhao,Zhengyan.
Nitric oxide (NO), synthesized as needed by NO synthase (NOS), is involved in spinogenesis and synaptogenesis. Immature spine morphology is characteristic of fragile X syndrome (FXS). The objective of this research was to investigate and compare changes of postnatal neuronal NOS (nNOS) expression in the hippocampus of male fragile X mental retardation 1 gene knockout mice (FMR1 KO mice, the animal model of FXS) and male wild-type mice (WT) at postnatal day 7 (P7), P14, P21, and P28. nNOS mRNA levels were analyzed by real-time quantitative PCR (N = 4-7) and nNOS protein was estimated by Western blot (N = 3) and immunohistochemistry (N = 1). In the PCR assessment, primers 5’-GTGGCCATCGTGTCCTACCATAC-3’ and 5’-GTTTCGAGGCAGGTGGAAGCTA-3’ were used for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FXS; NNOS; NO; Dendritic spine; Hippocampus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200017

Eucalyptol, an essential oil, reduces contractile activity in rat cardiac muscle
Provedor de dados: 56
Autores: Soares,M.C.M.S.; Damiani,C.E.N.; Moreira,C.M.; Stefanon,I.; Vassallo,D.V.
Eucalyptol is an essential oil that relaxes bronchial and vascular smooth muscle although its direct actions on isolated myocardium have not been reported. We investigated a putative negative inotropic effect of the oil on left ventricular papillary muscles from male Wistar rats weighing 250 to 300 g, as well as its effects on isometric force, rate of force development, time parameters, post-rest potentiation, positive inotropic interventions produced by Ca2+ and isoproterenol, and on tetanic tension. The effects of 0.3 mM eucalyptol on myosin ATPase activity were also investigated. Eucalyptol (0.003 to 0.3 mM) reduced isometric tension, the rate of force development and time parameters. The oil reduced the force developed by steady-state contractions (50%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptol; Myocardial contractility; Calcium; Isoproterenol; Tetanic tension.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300017

Memory-impairing effects of local anesthetics in an elevated plus-maze test in mice
Provedor de dados: 56
Autores: Blatt,S.L.; Takahashi,R.N.
Post-training intracerebroventricular administration of procaine (20 µg/µl) and dimethocaine (10 or 20 µg/µl), local anesthetics of the ester class, prolonged the latency (s) in the retention test of male and female 3-month-old Swiss albino mice (25-35 g body weight; N = 140) in the elevated plus-maze (mean ± SEM for 10 male mice: control = 41.2 ± 8.1; procaine = 78.5 ± 10.3; 10 µg/µl dimethocaine = 58.7 ± 12.3; 20 µg/µl dimethocaine = 109.6 ± 5.73; for 10 female mice: control = 34.8 ± 5.8; procaine = 55.3 ± 13.4; 10 µg/µl dimethocaine = 59.9 ± 12.3 and 20 µg/µl dimethocaine = 61.3 ± 11.1). However, lidocaine (10 or 20 µg/µl), an amide class type of local anesthetic, failed to influence this parameter. Local anesthetics at the dose range used did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lidocaine; Procaine; Dimethocaine; Elevated plus-maze; Memory; Dopamine.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400013

Interruption of recently induced immune responses by oral administration of antigen
Provedor de dados: 56
Autores: Conde,A.A.; Stransky,B.; Faria,A.M.C.; Vaz,N.M.
Interest in oral tolerance has been renewed in the last few years as a possibility of intervention in human autoimmune diseases. An obstacle in this direction is that, although easily induced in animals virgin of contact with the antigen, oral tolerance becomes hard to induce in previously immunized animals. The present results show that there is an early period after primary immunization in which prolonged oral exposure to the antigen may arrest ongoing immune responses. Beyond this period, oral exposures to the antigen become ineffective and may actually boost immune responses. The end of the susceptible period coincides with the emergence of free specific antibodies in serum. However, the previous administration of purified anti-ovalbumin antibodies (40...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Suppression; Antibody response; Oral tolerance; Ovalbumin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300008

Effect of the GABA B agonist baclofen on dipyrone-induced delayed gastric emptying in rats
Provedor de dados: 56
Autores: Collares,E.F.; Vinagre,A.M.
Dipyrone administered intravenously (iv) or intracerebroventricularly (icv) delays gastric emptying (GE) in rats. Gamma-aminobutyric acid (GABA) is the most potent inhibitory neurotransmitter of the central nervous system. The objective of the present study was to determine the effect of icv baclofen, a GABA B receptor agonist, on delayed GE induced by dipyrone. Adult male Wistar rats received a saline test meal containing phenol red as a marker. GE was indirectly evaluated by determining the percent of gastric retention (%GR) of the meal 10 min after orogastric administration. In the first experiment, the animals were injected iv with vehicle (Civ) or 80 mg/kg (240 µmol/kg) dipyrone (Dp iv), followed by icv injection of 10 µl vehicle (bac0), or 0.5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Dipyrone; GABA; GABA B receptors; Baclofen.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100015

Biodistribution of 99m technetium- labeled creatinine in healthy rats
Provedor de dados: 56
Autores: Yilmaz,O.; Yurt Lambrecht,F.; Soylu,A.; Durkan,K.; Kavukcu,S.
The distribution of creatinine, one of the toxic guanidine compounds, in various tissues has not been studied in detail by using radiolabeled creatinine. Our objective was to investigate the biodistribution of creatinine labeled with 99m technetium (99mTc) by the stannous (II) chloride method in healthy male Wistar rats. Quality controls were carried out by radio thin layer chromatography, high-performance liquid chromatography, and paper electrophoresis. The labeling yield was 85 ± 2% under optimum conditions (pH 7 and 100 µg stannous chloride). Rats (N = 12) were injected intravenously with 99mTc-creatinine and their blood and visceral organs were evaluated for 99mTc-creatinine uptake as percent of the injected dose per gram wet weight of each tissue...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Biodistribution; Creatinine; 99mTechnetium; 99mTc-creatinine; Rat.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600009

Effect of Helicobacter pylori infection and acid blockade by lansoprazole on clarithromycin bioavailability
Provedor de dados: 56
Autores: Ortiz,R.A.M.; Calafatti,S.A.; Moraes,L.A.; Deguer,M.; Ecclissato,C.C.; Marchioretto,M.A.M.; Ribeiro,M.L.; Bernasconi,G.; Pedrazzoli Jr,J.
The effect of proton pump inhibitors and Helicobacter pylori infection on the bioavailability of antibiotics is poorly understood. We determined the effects of 5-day oral administration of 60 mg lansoprazole on the bioavailability of clarithromycin in individuals with and without H. pylori infection. Thirteen H. pylori-infected and 10 non-infected healthy volunteers were enrolled in a study with an open-randomized two-period crossover design and a 21-day washout period between phases. Plasma concentrations of clarithromycin in subjects with and without lansoprazole pre-treatment were measured by liquid chromatography coupled to a tandem mass spectrometer. Clarithromycin Cmax and AUC0-10 h were significantly reduced after lansoprazole administration. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clarithromycin; Helicobacter pylori; Lansoprazole; Peptic ulcer; Pharmacokinetics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300014

Response to an oral calcium load in nephrolithiasis patients with fluctuating parathyroid hormone and ionized calcium levels
Provedor de dados: 56
Autores: Gomes,S.A.; Lage,A.; Lazaretti-Castro,M.; Vieira,J.G.H.; Heilberg,I.P.
the response to an oral calcium load test was assessed in 17 hypercalciuric nephrolithiasis patients who presented elevated parathyroid hormone (PTH) irrespective of the ionized calcium (sCa2+) levels. Blood samples were collected at baseline (0 min) and at 60 and 180 min after 1 g calcium load for serum PTH, total calcium, sCa2+, and 1.25(OH)2D3 determinations. According to the sCa2+ level at baseline, patients were classified as normocalcemic (N = 9) or hypercalcemic (N = 8). Six healthy subjects were also evaluated as controls. Bone mineral density was reduced in 14/17 patients. In the normocalcemic group, mean PTH levels at 0, 60 and 180 min (95 ± 76, 56 ± 40, 57 ± 45 pg/ml, respectively) did not differ from the hypercalcemic group (130 ± 75, 68 ± 35,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parathyroid hormone; Calcium; Hyperparathyroidism; Hypercalciuria; Nephrolithiasis; Bone mineral density.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900013

Effects of autacoid inhibitors and of an antagonist on malaria infection in mice
Provedor de dados: 56
Autores: Iwalewa,E.O.; Agbani,E.O.
The effects of p-chlorophenylalanine, an inhibitor of serotonin synthesis, indomethacin, an inhibitor of prostaglandin synthesis, cyproheptadine, a serotonin, bradykinin and histamine antagonist, were assessed separately and in combination with chloroquine (CQ) in Vom strains of Swiss albino mice (18-22 g) of either sex infected intraperitoneally with 1 x 10(7) Plasmodium yoelii nigeriensis-induced malaria. As prophylactic, these agents reduced from 31.9 ± 4.5 to 16.1 ± 8.1% the level of parasitemia relative to control but had no appreciable activity as curative agents when administered subcutaneously once daily for 4 days after 72 h of parasites innoculum in vivo. However, CQ alone and the combination of these agents with CQ in curative and prophylactic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autacoid inhibitors; Autacoid antagonist; Malaria infection; Malarial prophylaxis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800010

Characterization and analysis of the outcome of adults with acute myeloid leukemia treated in a Brazilian University hospital over three decades
Provedor de dados: 56
Autores: Souto Filho,J.T.; Portugal,R.D.; Loureiro,M.; Pulcheri,W.; Nucci,M.
We evaluated the outcome of 227 patients with acute myeloid leukemia during three decades (period 1 - 1980’s, N = 89; period 2 - 1990’s, N = 73; period 3 - 2000’s, N = 65) at a single institution. Major differences between the three groups included a higher median age, rates of multilineage dysplasia and co-morbidities, and a lower rate of clinical manifestations of advanced leukemia in recent years. The proportion of patients who received induction remission chemotherapy was 66, 75, and 85% for periods 1, 2, and 3, respectively (P = 0.04). The median survival was 40, 77, and 112 days, and the 5-year overall survival was 7, 13, and 22%, respectively (P = 0.01). The median disease-free survival was 266, 278, and 386 days (P = 0.049). Survival expectation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myeloid leukemia; Survival; Prognosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700008

Effect of eccentric training on mitochondrial function and oxidative stress in the skeletal muscle of rats
Provedor de dados: 56
Autores: Silva,L.A.; Bom,K.F.; Tromm,C.B.; Rosa,G.L.; Mariano,I.; Pozzi,B.G.; Tuon,T.; Stresck,E.L.; Souza,C.T.; Pinho,R.A.
The objective of the present study was to investigate the effects of eccentric training on the activity of mitochondrial respiratory chain enzymes, oxidative stress, muscle damage, and inflammation of skeletal muscle. Eighteen male mice (CF1) weighing 30-35 g were randomly divided into 3 groups (N = 6): untrained, trained eccentric running (16°; TER), and trained running (0°) (TR), and were submitted to an 8-week training program. TER increased muscle oxidative capacity (succinate dehydrogenase and complexes I and II) in a manner similar to TR, and TER did not decrease oxidative damage (xylenol and creatine phosphate) but increased antioxidant enzyme activity (superoxide dismutase and catalase) similar to TR. Muscle damage (creatine kinase) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eccentric training; Mitochondrial enzyme activity; Oxidative stress; Muscle damage; Inflammation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100014

A connection between extracellular matrix and hormonal signals during the development of the human fetal adrenal gland
Provedor de dados: 56
Autores: Chamoux,E.; Otis,M.; Gallo-Payet,N.
The human adrenal cortex, involved in adaptive responses to stress, body homeostasis and secondary sexual characters, emerges from a tightly regulated development of a zone-specific secretion pattern during fetal life. Its development during fetal life is critical for the well being of pregnancy, the initiation of delivery, and even for an adequate adaptation to extra-uterine life. As early as from the sixth week of pregnancy, the fetal adrenal gland is characterized by a highly proliferative zone at the periphery, a concentric migration accompanied by cell differentiation (cortisol secretion) and apoptosis in the central androgen-secreting fetal zone. After birth, a strong reorganization occurs in the adrenal gland so that it better fulfills the newborn's...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenal gland; Development; Human fetus; Extracellular matrix; Angiotensin II; Adrenocorticotropin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000006

Association between serotonin 2C receptor gene (HTR2C) polymorphisms and psychopathological symptoms in children and adolescents
Provedor de dados: 56
Autores: Paes,L.A.; Torre,O.H. Della; Henriques,T.B.; de Mello,M.P.; Celeri,E.H.R.V.; Dalgalarrondo,P.; Guerra Júnior,G.; dos Santos Júnior,A.
Serotonin 2C receptors (5HT2C) are involved in serotonin-driven dynamic equilibrium adjustments responsible for homeostatic stability in brain structures that modulate behavior and emotions. Single nucleotide polymorphisms (SNPs) from the serotonin 2C receptor gene (HTR2C) have been associated with several neurological and mental disorders, including abnormalities in cognitive and emotional processes. The aim of this study was to evaluate the association between the rs6318 SNP of the HTR2C gene and behavioral characteristics exhibited by children and adolescents based on the Child Behavior Checklist (CBCL/6-18) inventory. Eighty-five psychiatric outpatients between 8 and 18 years of age underwent genotyping of the rs6318 SNP. The CBCL/6-18 scale was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic polymorphism; Serotonin; CBCL/6-18; Children; Adolescents; HTR2C.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800611

Effect of physical training on liver expression of activin A and follistatin in a nonalcoholic fatty liver disease model in rats
Provedor de dados: 56
Autores: Silva,R.N.; Bueno,P.G.; Avó,L.R.S.; Nonaka,K.O.; Selistre-Araújo,H.S.; Leal,A.M.O.
Nonalcoholic fatty liver disease (NAFLD) is characterized by fat accumulation in the liver and is associated with obesity and insulin resistance. Activin A is a member of the transforming growth factor beta (TGF)-β superfamily and inhibits hepatocyte growth. Follistatin antagonizes the biological actions of activin. Exercise is an important therapeutic strategy to reduce the metabolic effects of obesity. We evaluated the pattern of activin A and follistatin liver expression in obese rats subjected to swimming exercise. Control rats (C) and high-fat (HF) diet-fed rats were randomly assigned to a swimming training group (C-Swim and HF-Swim) or a sedentary group (C-Sed and HF-Sed). Activin βA subunit mRNA expression was significantly higher in HF-Swim than in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Activin A; Follistatin; Steatosis; Nonalcoholic fatty liver disease; Obesity; Exercise.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900746

Computerized analysis of isometric tension studies provides important additional information about vasomotor activity
Provedor de dados: 56
Autores: Vincent,M.B.; White,L.R.; Bakken,I.J.; Sjaavaag,I.
Concentration-response curves of isometric tension studies on isolated blood vessels are obtained traditionally. Although parameters such as Imax, EC50 and pA2 may be readily calculated, this method does not provide information on the temporal profile of the responses or the actual nature of the reaction curves. Computerized data acquisition systems can be used to obtain average data that represent a new source of otherwise inaccessible information, since early and late responses may be observed separately in detail
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Vasoactivity; Digitalization; Substance P; Acetylcholine; VIP; CGRP.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000015

Effect of pilocarpine mouthwash on salivary flow
Provedor de dados: 56
Autores: Bernardi,R.; Perin,C.; Becker,F.L.; Ramos,G.Z.; Gheno,G.Z.; Lopes,L.R.; Pires,M.; Barros,H.M.T.
Pilocarpine is a cholinergic agonist that increases salivary flow and has been used to treat xerostomia. Oral intake is the most frequent route of administration. Adverse effects are dose-dependent and include sudoresis, facial blushing and increased urinary frequency. The objective of the present study was to evaluate the effects of topical pilocarpine solutions as mouthwashes on salivary flow and their adverse effects on healthy subjects. Forty volunteers received 10 ml 0.5, 1 and 2% pilocarpine solutions or 0.9% saline in a randomized, double-blind, placebo-controlled manner. Salivation was measured before and 45, 60 and 75 min after mouth rinsing for 1 min with 10 ml of saline or pilocarpine solutions. Vital signs were measured and ocular,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pilocarpine; Mouth rinsing; Mouthwash; Xerostomia; Salivation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100015

Expression of beclin 1 in primary salivary adenoid cystic carcinoma and its relation to Bcl-2 and p53 and prognosis
Provedor de dados: 56
Autores: Jiang,L.C.; Huang,S.Y.; Zhang,D.S.; Zhang,S.H.; Li,W.G.; Zheng,P.H.; Chen,Z.W.
Beclin 1 plays a critical role in autophagy and functions as a haploinsufficient tumor suppressor. The expression and prognostic significance of beclin 1 in head and neck adenoid cystic carcinoma (ACC) are largely unexplored. Therefore, we investigated the expression of beclin 1, Bcl-2, and p53 in head and neck ACC tissue. Tissue samples from 35 cases (15 females, 20 males) of head and neck ACC were utilized for immunohistochemistry. Beclin 1 expression was observed in 32 cases (91.4%) and considered to be high in 15 cases (42.9%) and low in 20 cases (57.1%). Beclin 1 expression was significantly correlated with a histological growth pattern (P=0.046) and histological grade (P=0.037). Beclin 1 expression was inversely correlated with Bcl-2 expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adenoid cystic carcinoma; Autophagy; Beclin 1; Bcl-2; P53.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300252

Immunological basis of septal fibrosis of the liver in Capillaria hepatica-infected rats
Provedor de dados: 56
Autores: Lemos,Q.T.; Magalhães-Santos,I.F.; Andrade,Z.A.
Rats infected with the helminth Capillaria hepatica regularly develop septal fibrosis of the liver similar to that induced by repeated ip injections of pig serum. Fibrosis starts when the focal parasitic lesions begin to show signs of resorption, thus suggesting an immunologically mediated pathogenesis of this fibrosis. To explore this possibility, the development of C. hepatica-related hepatic fibrosis was observed in rats exposed to worm antigens from the first neonatal day onward. Wistar rats (150 g) were either injected ip with an extract of C. hepatica eggs (protein concentration: 1 mg/ml) or received immature eggs by gavage from the first neonatal day until adult life and were then infected with 500 embryonated eggs. Changes were monitored on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capillaria hepatica model; Hepatic fibrosis; Immunological tolerance.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900009

Screening of antibacterial extracts from plants native to the Brazilian Amazon Rain Forest and Atlantic Forest
Provedor de dados: 56
Autores: Suffredini,I.B.; Sader,H.S.; Gonçalves,A.G.; Reis,A.O.; Gales,A.C.; Varella,A.D.; Younes,R.N.
More than 20% of the world's biodiversity is located in Brazilian forests and only a few plant extracts have been evaluated for potential antibacterial activity. In the present study, 705 organic and aqueous extracts of plants obtained from different Amazon Rain Forest and Atlantic Forest plants were screened for antibacterial activity at 100 µg/ml, using a microdilution broth assay against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli. One extract, VO581, was active against S. aureus (minimum inhibitory concentration (MIC) = 140 µg/ml and minimal bactericidal concentration (MBC) = 160 µg/ml, organic extract obtained from stems) and two extracts were active against E. faecalis, SM053 (MIC = 80 µg/ml and MBC = 90...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant extract; Antibacterial activity; Screening; Amazon Rain Forest; Atlantic Forest.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300015

Adipose tissue is less responsive to food restriction anti-inflammatory effects than liver, muscle, and brain in mice
Provedor de dados: 56
Autores: Antunes,M.M.; de Almeida-Souza,C.B.; Godoy,G.; Crisma,A.R.; Masi,L.N.; Curi,R.; Bazotte,R.B.
High caloric intake promotes chronic inflammation, insulin resistance, and chronic diseases such as type-2 diabetes, which may be prevented by food restriction (FR). The effect of FR on expression of pro-inflammatory and anti-inflammatory genes in adipose tissue, liver, muscle, and brain was compared. Male Swiss mice were submitted to FR (FR group) or had free access to food (control group) during 56 days. The liver, gastrocnemius muscle, brain, and epididymal white adipose tissue (WAT) were collected for analysis of gene expressions. FR attenuated inflammation in the liver, brain, and gastrocnemius muscle but did not markedly change inflammatory gene expression in epididymal WAT. We concluded that adipose tissue was less responsive to FR in terms of gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caloric restriction; Pro-inflammatory cytokines; Anti-inflammatory cytokines; Acetyl-CoA carboxylase; Disease prevention.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100612

Bioinformatics analysis of biomarkers and transcriptional factor motifs in Down syndrome
Provedor de dados: 56
Autores: Kong,X.D.; Liu,N.; Xu,X.J.
In this study, biomarkers and transcriptional factor motifs were identified in order to investigate the etiology and phenotypic severity of Down syndrome. GSE 1281, GSE 1611, and GSE 5390 were downloaded from the gene expression ominibus (GEO). A robust multiarray analysis (RMA) algorithm was applied to detect differentially expressed genes (DEGs). In order to screen for biological pathways and to interrogate the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, the database for annotation, visualization, and integrated discovery (DAVID) was used to carry out a gene ontology (GO) function enrichment for DEGs. Finally, a transcriptional regulatory network was constructed, and a hypergeometric distribution test was applied to select for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Down syndrome; Candidate gene; Biomarker; Transcriptional factor motifs.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000834

The reciprocal interaction between sleep and type 2 diabetes mellitus: facts and perspectives
Provedor de dados: 56
Autores: Martins,R.C.; Andersen,M.L.; Tufik,S.
Type 2 diabetes mellitus is a systemic disease characterized by intolerance to glucose and peripheral resistance to insulin. This endocrine disease affects fundamental mechanisms of the central nervous system and jeopardizes the balance of vital functions such as the cardiovascular and circadian rhythm. The increased prevalence of metabolic disorders in our society is aggravated by endemic voluntary postponement of bedtime and by the current sedentary lifestyle, leading to epidemic proportions of obese people. Diabetes and chronic loss of sleep share the fact that both affect millions and one is detrimental to the other. Indeed, sleep deficits have marked modulatory effects on glucose metabolism and insulin sensitivity and foster metabolic syndrome that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Metabolic syndrome; Obesity; Apnea; Sleep.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300001

The calcium-modulated proteins, S100A1 and S100B, as potential regulators of the dynamics of type III intermediate filaments
Provedor de dados: 56
Autores: Garbuglia,M.; Verzini,M.; Sorci,G.; Bianchi,R.; Giambanco,I.; Agneletti,A.L.; Donato,R.
The Ca2+-modulated, dimeric proteins of the EF-hand (helix-loop-helix) type, S100A1 and S100B, that have been shown to inhibit microtubule (MT) protein assembly and to promote MT disassembly, interact with the type III intermediate filament (IF) subunits, desmin and glial fibrillary acidic protein (GFAP), with a stoichiometry of 2 mol of IF subunit/mol of S100A1 or S100B dimer and an affinity of 0.5-1.0 µM in the presence of a few micromolar concentrations of Ca2+. Binding of S100A1 and S100B results in inhibition of desmin and GFAP assemblies into IFs and stimulation of the disassembly of preformed desmin and GFAP IFs. S100A1 and S100B interact with a stretch of residues in the N-terminal (head) domain of desmin and GFAP, thereby blocking the head-to-tail...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: S100A1; S100B; Intermediate filaments; Calcium; Regulatory effects; Assembly-disassembly.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000001

Identification of Mycobacterium bovis antigens by analysis of bovine T-cell responses after infection with a virulent strain
Provedor de dados: 56
Autores: Alito,A.; McNair,J.; Girvin,R.M.; Zumarraga,M.; Bigi,F.; Pollock,J.M.; Cataldi,A.
Purification and characterization of individual antigenic proteins are essential for the understanding of the pathogenic mechanisms of mycobacteria and the immune response against them. In the present study, we used anion-exchange chromatography to fractionate cell extracts and culture supernatant proteins from Mycobacterium bovis to identify T-cell-stimulating antigens. These fractions were incubated with peripheral blood mononuclear cells (PBMC) from M. bovis-infected cattle in lymphoproliferation assays. This procedure does not denature proteins and permits the testing of mixtures of potential antigens that could be later identified. We characterized protein fractions with high stimulation indices from both culture supernatants and cell extracts....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine tuberculosis; Antigens; Mycobacterium bovis; Cellular immunity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100011

Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense
Provedor de dados: 56
Autores: Nishikawa,C.Y.; Araújo,L.M.; Kadowaki,M.A.S.; Monteiro,R.A.; Steffens,M.B.R.; Pedrosa,F.O.; Souza,E.M.; Chubatsu,L.S.
Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize. The expression of genes responsible for nitrogen fixation (nif genes) in this bacterium is dependent on the transcriptional activator NifA. This protein contains three structural domains: the N-terminal domain is responsible for the negative control by fixed nitrogen; the central domain interacts with the RNA polymerase σ54 co-factor and the C-terminal domain is involved in DNA binding. The central and C-terminal domains are linked by the interdomain linker (IDL). A conserved four-cysteine motif encompassing the end of the central domain and the IDL is probably involved in the oxygen-sensitivity of NifA. In the present study,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Biological nitrogen fixation; Azospirillum brasilense; NifA protein.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200004

c-Fos expression induced by electroacupuncture at the Zusanli point in rats submitted to repeated immobilization
Provedor de dados: 56
Autores: Medeiros,M.A.; Canteras,N.S.; Suchecki,D.; Mello,L.E.A.M.
In laboratory animals, acupuncture needs to be performed on either anesthetized or, if unanesthetized, restrained subjects. Both procedures up-regulate c-Fos expression in several areas of the central nervous system, representing therefore a major pitfall for the assessment of c-Fos expression induced by electroacupuncture. Thus, in order to reduce the effect of acute restraint we used a protocol of repeated restraint for the assessment of the brain areas activated by electroacupuncture in adult male Wistar rats weighing 180-230 g. Repeated immobilization protocols (6 days, 1 h/day and 13 days, 2 h/day) were used to reduce the effect of acute immobilization stress on the c-Fos expression induced by electroacupuncture at the Zusanli point (EA36S). Animals...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acupuncture; Zusanli point; Immediate early genes; C-Fos; Restraint; Habituation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200009

Tracheal instillation of urban PM2.5 suspension promotes acute cardiac polarization changes in rats
Provedor de dados: 56
Autores: Maatz,L.F.; Wood,G.J.A.; Rivero,D.H.R.F.; Saldiva,P.H.N.
The mechanisms by which PM2.5 increases cardiovascular mortality are not fully identified. Autonomic alterations are the current main hypotheses. Our objective was to determine if PM2.5 induces acute cardiac polarization alterations in healthy Wistar rats. PM2.5 samples were collected on polycarbonate filters. Solutions containing 10, 20, and 50 µg PM2.5 were administered by tracheal instillation. P wave duration decreased significantly at 20 µg (0.99 ± 0.06, 0.95 ± 0.06, and 0.96 ± 0.07; P &lt; 0.001), and 50 µg (0.98 ± 0.06, 0.98 ± 0.07, and 0.96 ± 0.08; 60, 90 and 120 min, respectively) compared to blank filter solution (P &lt; 0.001). PR interval duration decreased significantly at 20 µg (0.99 ± 0.06, 0.98 ± 0.07, and 0.97 ± 0.08) and 50 µg (0.99 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ECG alterations; Particulate matter; PM2.5; Wave segments; Tracheal instillation; Cardiovascular disease.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200009

Omphaloceles in combination with a self-made hemispherical cushion: a report of 12 cases
Provedor de dados: 56
Autores: Huang,Guoxian; Ma,Lili; Wu,Yonglong.
The aim of this study was to discuss the curative effect of applying “capsule-reserved normal saline bag and self-made hemi-spherical cushion oppression” for treating giant omphaloceles. Twelve patients with giant omphaloceles who were admitted to our hospital between January 2008 and June 2016 were selected for treatment as follows: a capsule-reserved normal saline bag was used to promote the gradual return of the abdominal contents into the abdominal cavity in phase I, and a self-made hemi-spherical cushion was used for compression combined with a local dressing change in phase II to treat the giant omphaloceles without surgical treatment. All 12 patients in this group were cured, and after follow-up visits for &gt;10 months, they had no abdominal...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Giant omphalocele; Non-surgical treatment in phase II; Treatment in stages; Staged treatment.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000501

Increased expression of tissue factor and protease-activated receptor-1 does not correlate with thrombosis in human lung adenocarcinoma
Provedor de dados: 56
Autores: de Meis,E.; Azambuja,D.; Ayres-Silva,J.P.; Zamboni,M.; Pinheiro,V.R.; Levy,R.A.; Monteiro,R.Q.
A correlation between cancer and hypercoagulability has been described for more than a century. Patients with cancer are at increased risk for thrombotic complications and the clotting initiator protein, tissue factor (TF), is possibly involved in this process. Moreover, TF may promote angiogenesis and tumor growth. In addition to TF, thrombin seems to play a relevant role in tumor biology, mainly through activation of protease-activated receptor-1 (PAR-1). In the present study, we prospectively studied 39 lung adenocarcinoma patients in relation to the tumor expression levels of TF and PAR-1 and their correlation with thrombosis outcome and survival. Immunohistochemical analysis showed TF positivity in 22 patients (56%), most of them in advanced stages...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung adenocarcinoma; Thrombosis; Tissue factor; Protease-activated receptor.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400012

Renal dysfunction in patients with sickle cell anemia or sickle cell trait
Provedor de dados: 56
Autores: Sesso,R.; Almeida,M.A.; Figueiredo,M.S.; Bordin,J.O.
Patients with sickle cell anemia (Hb SS) or sickle cell trait (Hb AS) may present several types of renal dysfunction; however, comparison of the prevalence of these abnormalities between these two groups and correlation with the duration of disease in a large number of patients have not been thoroughly investigated. In a cross-sectional study using immunoenzymometric assays to measure tubular proteinuria, microalbuminuria, measurement of creatinine clearance, urinary osmolality and analysis of urine sediment, we evaluated glomerular and tubular renal function in 106 adults and children with Hb SS (N = 66) or Hb AS (N = 40) with no renal failure (glomerular filtration rate (GFR) &amp;gt;85 ml/min). The percentage of individuals with microalbuminuria was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microalbuminuria; Renal failure; Renal function; Sickle cell disease.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000004

Expression of Fos protein in the rat central nervous system in response to noxious stimulation: effects of chronic inflammation of the superior cervical ganglion
Provedor de dados: 56
Autores: Laudanna,A.; Nogueira,M.I.; Mariano,M.
The aim of this study was to investigate the possible interactions between the nociceptive system, the sympathetic system and the inflammatory process. Thus, the superior cervical ganglion of rats was submitted to chronic inflammation and Fos expression was used as a marker for neuronal activity throughout central neurons following painful peripheral stimulation. The painful stimulus consisted of subcutaneously injected formalin applied to the supra-ocular region. Fos-positive neurons were identified by conventional immunohistochemical techniques, and analyzed from the obex through the cervical levels of the spinal cord. In the caudal sub-nucleus of the spinal trigeminal nuclear complex, the number of Fos-positive neurons was much higher in rats with...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Autonomic nervous system; Pain; Sympathetic system; Trigeminal complex.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600019

Hydrolysis of xylans by enzyme systems from solid cultures of Trichoderma harzianum strains
Provedor de dados: 56
Autores: Silveira,F.Q.P.; Ximenes,F.A.; Cacais,A.O.G.; Milagres,A.M.F.; Medeiros,C.L.; Puls,J.; Filho,E.X.F.
Xylanase activity was isolated from crude extracts of Trichoderma harzianum strains C and 4 grown at 28oC in a solid medium containing wheat bran as the carbon source. Enzyme activity was demonstrable in the permeate after ultrafiltration of the crude extracts using an Amicon system. The hydrolysis patterns of different xylans and paper pulps by xylanase activity ranged from xylose, xylobiose and xylotriose to higher xylooligosaccharides. A purified ß-xylosidase from the Trichoderma harzianum strain released xylose, xylobiose and xylotriose from seaweed, deacetylated, oat spelt and birchwood xylans. The purified enzyme was not active against acetylated xylan and catalyzed the hydrolysis of xylooligosaccharides, including xylotriose, xylotetraose and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Trichoderma; Xylan; Xylanase.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800003

Intramyocardial implantation of differentiated rat bone marrow mesenchymal stem cells enhanced by TGF-β1 improves cardiac function in heart failure rats
Provedor de dados: 56
Autores: Lv,Y.; Liu,B.; Wang,H.P.; Zhang,L.
The present study tested the hypotheses that i) transforming growth factor beta 1 (TGF-β1) enhances differentiation of rat bone marrow mesenchymal stem cells (MSCs) towards the cardiomyogenic phenotype and ii) intramyocardial implantation of the TGF-β1-treated MSCs improves cardiac function in heart failure rats. MSCs were treated with different concentrations of TGF-β1 for 72 h, and then morphological characteristics, surface antigens and mRNA expression of several transcription factors were assessed. Intramyocardial implantation of these TGF-β1-treated MSCs to infarcted heart was also investigated. MSCs were initially spindle-shaped with irregular processes. On day 28 after TGF-β1 treatment, MSCs showed fusiform shape, orientating parallel with one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow mesenchymal stem cell; Transforming growth factor beta1; Cardiomyocytes; Differentiation; Intramyocardial implantation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600603

Cost-effective analysis of different algorithms for the diagnosis of hepatitis C virus infection
Provedor de dados: 56
Autores: Barreto,A.M.E.C.; Takei,K.; E.C.,Sabino; Bellesa,M.A.O.; Salles,N.A.; Barreto,C.C.; Nishiya,A.S.; Chamone,D.F.
We compared the cost-benefit of two algorithms, recently proposed by the Centers for Disease Control and Prevention, USA, with the conventional one, the most appropriate for the diagnosis of hepatitis C virus (HCV) infection in the Brazilian population. Serum samples were obtained from 517 ELISA-positive or -inconclusive blood donors who had returned to Fundação Pró-Sangue/Hemocentro de São Paulo to confirm previous results. Algorithm A was based on signal-to-cut-off (s/co) ratio of ELISA anti-HCV samples that show s/co ratio ³95% concordance with immunoblot (IB) positivity. For algorithm B, reflex nucleic acid amplification testing by PCR was required for ELISA-positive or -inconclusive samples and IB for PCR-negative samples. For algorithm C, all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C virus; Immunoblot; ELISA; Polymerase chain reaction; Algorithm; Diagnosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200009

Different stress modalities result in distinct steroid hormone responses by male rats
Provedor de dados: 56
Autores: Andersen,M.L.; Bignotto,M.; Machado,R.B.; Tufik,S.
Since both paradoxical sleep deprivation (PSD) and stress alter male reproductive function, the purpose of the present study was to examine the influence of PSD and other stressors (restraint, electrical footshock, cold and forced swimming, N = 10 per group) on steroid hormones in adult Wistar male rats. Rats were submitted to chronic stress for four days. The stressors (footshock, cold and forced swimming) were applied twice a day, for periods of 1 h at 9:00 and 16:00 h. Restrained animals were maintained in plastic cylinders for 22 h/day whereas PSD was continuous. Hormone determination was measured by chemiluminescent enzyme immunoassay (testosterone), competitive immunoassay (progesterone) and by radioimmunoassay (corticosterone, estradiol, estrone)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paradoxical sleep deprivation; Stress; Testosterone; Progesterone; Estrogen; Corticosterone.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600003

A broad pH range and processive chitinase from a metagenome library
Provedor de dados: 56
Autores: Thimoteo,S.S.; Glogauer,A.; Faoro,H.; de Souza,E.M.; Huergo,L.F.; Moerschbacher,B.M.; Pedrosa,F.O.
Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene named metachi18A. The metachi18A gene was subcloned and overexpressed in Escherichia coli BL21 and the MetaChi18A chitinase was purified by affinity chromatography as a 6xHis-tagged fusion protein. The MetaChi18A enzyme is a 92-kDa protein with a conserved active site domain of glycosyl hydrolases family 18. It hydrolyses colloidal chitin with an optimum pH of 5 and temperature of 50°C. Moreover, the enzyme...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitinase; Metagenomic; Aeromonas; Kinetics.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100602

The 9-O-acetyl GD3 gangliosides are expressed by migrating chains of subventricular zone neurons in vitro
Provedor de dados: 56
Autores: Miyakoshi,L.M.; Mendez-Otero,R.; Hedin-Pereira,C.
Neurons from the anterior subventricular zone (SVZ) of the cerebral cortex migrate tangentially to become interneurons in the olfactory bulb during development and in adult rodents. This migration was defined as neuronophilic, independent of a radial glial substrate. The cortical SVZ and the rostral migratory stream to the olfactory bulb were shown to be rich in 9-O-acetyl GD3 gangliosides (9-O-acGD3), which have been previously shown to be implicated in gliophilic migration in the rodent cerebral cortex and cerebellum. In the present study, we performed SVZ explant cultures using rats during their first postnatal week to analyze the expression of these gangliosides in chain migration of neuronal precursors. We characterized migrating chains of these...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Subventricular zone; Migration; 9-O-acetylated gangliosides; Rostral migratory stream.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500016

Effect of toxin-g from Tityus serrulatus scorpion venom on gastric emptying in rats
Provedor de dados: 56
Autores: Bucaretchi,F.; Vinagre,A.M.; Chavéz-Olórtegui,C.; Collares,E.F.
The effect of toxin-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; from Tityus serrulatus scorpion venom on the gastric emptying of liquids was studied in 176 young adult male Wistar rats (2-3 months of age) divided into subgroups of 8 animals each. Toxin-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; was injected iv at doses of 25, 37.5, 50 or 100 µg/kg and the effect on gastric emptying was assessed 30 min and 8 h later. A time-course study was also performed by injecting 50 µg of toxin-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; /kg and measuring the effect on gastric emptying at times 0.25, 0.5, 1, 2, 4, 8, 24 and 48 h post-venom. Each envenomed animal was paired with its saline control and all received a saline test meal solution containing phenol red (60 µg/ml) as...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gastric emptying Tityus serrulatus scorpion venom toxin-&lt; FONT FACE=Symbol&gt; G&lt; /font&gt.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400009

Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices
Provedor de dados: 56
Autores: Torres,I.L.S.; Gamaro,G.D.; Silveira-Cucco,S.N.; Michalowski,M.B.; Corrêa,J.B.; Perry,M.L.S; Dalmaz,C.
It has been suggested that glucocorticoids released during stress might impair neuronal function by decreasing glucose uptake by hippocampal neurons. Previous work has demonstrated that glucose uptake is reduced in hippocampal and cerebral cortex slices 24 h after exposure to acute stress, while no effect was observed after repeated stress. Here, we report the effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices and on plasma glucose and corticosterone levels. Male adult Wistar rats were exposed to restraint 1 h/day for 50 days in the chronic model. In the acute model there was a single exposure. Immediately or 24 h after stress, the animals were sacrificed and the hippocampus and cerebral...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Restraint stress; Chronic stress; CO2 production; Glucose oxidation; Hippocampus; Cerebral cortex.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100013

Behavioral cross-sensitization between testosterone and fenproporex in adolescent and adult rats
Provedor de dados: 56
Autores: Conceição,C.Q.; Engi,S.A.; Cruz,F.C.; Planeta,C.S.
The abuse of psychoactive drugs is considered a global health problem. During the last years, a relevant number of studies have investigated the relationship between anabolic-androgenic steroids (AAS) and other psychoactive drugs. AAS, such as testosterone, can cause a dependence syndrome that shares many features with the classical dependence to psychoactive substances. Pre-clinical evidence shows that there are interactions between testosterone and psychoactive drugs, such as cocaine. However, few studies have been performed to investigate the effect of repeated testosterone treatment on behavioral effects of amphetamine derivatives, such as fenproporex. The purpose of the present study was to investigate the effects of repeated testosterone...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Behavioral sensitization; Fenproporex; Testosterone; Rats.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100603

Gene expression promoted by the SV40 DNA targeting sequence and the hypoxia-responsive element under normoxia and hypoxia
Provedor de dados: 56
Autores: Sacramento,C.B.; Moraes,J.Z.; Denapolis,P.M.A.; Han,S.W.
The main objective of the present study was to find suitable DNA-targeting sequences (DTS) for the construction of plasmid vectors to be used to treat ischemic diseases. The well-known Simian virus 40 nuclear DTS (SV40-DTS) and hypoxia-responsive element (HRE) sequences were used to construct plasmid vectors to express the human vascular endothelial growth factor gene (hVEGF). The rate of plasmid nuclear transport and consequent gene expression under normoxia (20% O2) and hypoxia (less than 5% O2) were determined. Plasmids containing the SV40-DTS or HRE sequences were constructed and used to transfect the A293T cell line (a human embryonic kidney cell line) in vitro and mouse skeletal muscle cells in vivo. Plasmid transport to the nucleus was monitored by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SV40-DTS; Hypoxia-responsive element; Transfection efficiency; Normoxia; Hypoxia; Ischemic diseases.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800005

Effects of metformin on the glycemic control, lipid profile, and arterial blood pressure of type 2 diabetic patients with metabolic syndrome already on insulin
Provedor de dados: 56
Autores: Mourão-Júnior,C.A.; Sá,J.R.; Guedes,O.M.S.; Dib,S.A.
Fifty-seven type 2 diabetic patients with metabolic syndrome and on insulin were assessed by a paired analysis before and 6 months after addition of metformin as combination therapy to evaluate the impact of the association on glycemic control, blood pressure, and lipid profile. This was a historical cohort study in which the files of type 2 diabetic patients with metabolic syndrome on insulin were reviewed. The body mass index (BMI), waist circumference, lipid profile, A1C level, fasting blood glucose level, daily dose of NPH insulin, systolic blood pressure, and diastolic blood pressure were assessed in each patient before the start of metformin and 6 months after the initiation of combination therapy. Glycemic control significantly improved (P &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes mellitus; Metabolic syndrome; Metformin; Blood pressure; Cholesterol; Triglycerides.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400009

PCR-based ribotyping of Staphylococcus aureus
Provedor de dados: 56
Autores: Oliveira,A.M.; Ramos,M.C.
Genotyping techniques are valuable tools for the epidemiologic study of Staphylococcus aureus infections in the hospital setting. Pulsed-field gel electrophoresis (PFGE) is the current method of choice for S. aureus strain typing. However, the method is laborious and requires expensive equipment. In the present study, we evaluated the natural polymorphism of the genomic 16S-23S rRNA region for genotyping purpose, by PCR-based ribotyping. Three primer pairs were tested to determine the size of amplicons produced and to obtain better discrimination with agar gel electrophoresis and ethidium bromide staining. The resolution of the typing system was determined using sets of bacteria obtained from clinical specimens from a large tertiary care hospital. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; PCR ribotyping; Genotyping; Methicillin-resistant Staphylococcus aureus.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200005

Genetic variations of EBV-LMP1 from nasopharyngeal carcinoma biopsies: potential loss of T cell epitopes
Provedor de dados: 56
Autores: Tang,Y.L.; Lu,J.H.; Cao,L.; Wu,M.H.; Peng,S.P.; Zhou,H.D.; Huang,C.; Yang,Y.X.; Zhou,Y.H.; Chen,Q.; Li,X.L.; Zhou,M.; Li,G.Y.
To find Epstein-Barr virus (EBV) strains with genetic variations of EBV latent membrane protein 1 (EBV-LMP1) from nasopharyngeal carcinoma (NPC), the full-length DNA of LMP1 genes from 21 NPC biopsies obtained in Hunan province in southern China was amplified and sequenced. Our sequences were compared to those previously reported by the Clustal V method. Results showed that all 21 sequences displayed two amino acid changes most frequently in LMP1 of CD4+ T cell epitopes at codons 144 (F®I, 21/21) and 212 (G®S, 19/21) or (G®N, 2/21). We also show that type A EBV strain is prevalent in the cases of NPC from Hunan province with a 30-bp 18/21 deletion, and we highlight that this deletion resulted in loss of one of the CD4+ T cell-restricted epitopes. The other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variation; Epitope; Immune recognition; Latent membrane protein 1; Tumorigenicity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200006

NADPH-diaphorase activity in area 17 of the squirrel monkey visual cortex: neuropil pattern, cell morphology and laminar distribution
Provedor de dados: 56
Autores: Franca,J.G.; do-Nascimento,J.L.M.; Picanço-Diniz,C.W.; Quaresma,J.A.S.; Silva,A.L.C.
We studied the distribution of NADPH-diaphorase activity in the visual cortex of normal adult New World monkeys (Saimiri sciureus) using the malic enzyme "indirect" method. NADPH-diaphorase neuropil activity had a heterogeneous distribution. In coronal sections, it had a clear laminar pattern that was coincident with Nissl-stained layers. In tangential sections, we observed blobs in supragranular layers of V1 and stripes throughout the entire V2. We quantified and compared the tangential distribution of NADPH-diaphorase and cytochrome oxidase blobs in adjacent sections of the supragranular layers of V1. Although their spatial distributions were rather similar, the two enzymes did not always overlap. The histochemical reaction also revealed two different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide synthase; Neocortex; NADPH-diaphorase; Cytochrome oxidase; Primates.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900008

Administration of interferon-g to pregnant rats reverses the depressed adjuvant-induced arthritis of their chronically Trypanosoma cruzi-infected offspring
Provedor de dados: 56
Autores: Didoli,G.; Revelli,S.; Davila,H.; Ferro,M.E.; Romero-Piffiguer,M.; Bottasso,O.
We demonstrated that administration of interferon gamma (IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;) to the inbred "l" strain of pregnant rats conferred partial resistance on their offspring to challenge with Trypanosoma cruzi. We now examine if this intervention also modifies the reportedly immunodepressed cellular responses which occur during chronic infection. Offspring were born to mothers undergoing one of the following procedures during gestation: subcutaneous injections of recombinant rat IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;, 50,000 IU/rat, five times/week for 3 weeks, which was started on the day of mating (IFN-Mo); infection with 106 trypomastigotes of T. cruzi at 7, 14, and 21 days after mating plus IFN-&lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interferon gamma; Adjuvant arthritis; T. cruzi; T cell subsets.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600011

Retraction notice for: “Risk factors for early-onset ventilator-associated pneumonia in aneurysmal subarachnoid hemorrhage patients” [Braz J Med Biol Res (2018) 51(7): e6830]
Provedor de dados: 56
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100951

The association of ACE gene D/I polymorphism with cardiovascular risk factors in a population from Rio de Janeiro
Provedor de dados: 56
Autores: Cardoso,R.L.; Nogueira,A.R.; Salis,L.H.A.; Ürményi,T.P.; Silva,R.; Moura-Neto,R.S.; Pereira,B.B.; Rondinelli,E.; de Souza e Silva,N.A.
Our aim was to determine the frequencies of the angiotensin-converting enzyme (ACE) gene alleles D and I and any associations to cardiovascular risk factors in a population sample from Rio de Janeiro, Brazil. Eighty-four adults were selected consecutively during a 6-month period from a cohort subgroup of a previous large cross-sectional survey in Rio de Janeiro. Anthropometric data and blood pressure measurements, echocardiogram, albuminuria, glycemia, lipid profile, and ACE genotype and serum enzyme activity were determined. The frequency of the ACE*D and I alleles in the population under study, determined by PCR, was 0.59 and 0.41, respectively, and the frequencies of the DD, DI, and II genotypes were 0.33, 0.51, and 0.16, respectively. No association...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin-converting enzyme gene polymorphism; Left ventricular hypertrophy; Angiotensin-converting enzyme activity; Albuminuria; Hypertension.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600013

Tai Chi versus routine exercise in patients with early- or mild-stage Parkinson's disease: a retrospective cohort analysis
Provedor de dados: 56
Autores: Li,Quanhao; Liu,Jinmei; Dai,Fei; Dai,Fengling.
Parkinson's disease cannot be cured but symptoms can be improved by making use of physical therapy. The objective of the study was to compare the effect of routine exercises and Tai Chi on physical and clinical performance in elderly people suffering from Parkinson's disease. Data from interviews, physical and clinical performance, and levodopa consumption of 500 patients with confirmed Parkinson's disease (severity level I to III) were collected and analyzed. Participants who received 80 min/day Tai Chi 3 times/week for 2 months were included in the Tai Chi (TC) group (n=250) and those who received 90 min/day routine exercise 3 times/week for 2 months were included in routine exercise (RE) group (n=250). Timed up-and-go, 50-foot speed walk, and functional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise; Fall; Levodopa; Parkinson's disease; Physical therapy; Tai Chi.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200606

Nasal CPAP in the delivery room for newborns with extremely low birth weight in a hospital in a developing country
Provedor de dados: 56
Autores: Gonçalves-Ferri,W.A.; Martinez,F.E.
The objective of this study was to determine the feasibility of the use of continuous positive airway pressure installed prophylactically in the delivery room (DR-CPAP), for infants with a birth weight between 500 and 1000 g in settings with limited resources. During 23 months, infants with a birth weight between 500 and 1000 g consecutively received DR-CPAP. A total of 33 infants with low birth weight were enrolled, 16 (48.5%) were females. Only 14 (42.4%) received antenatal corticosteroids and only 2 of those 14 (14.3%) infants weighing 500-750 g were not intubated in the delivery room, and apnea was given as the reason for intubation of these patients. Of the 19 infants in the 751-1000 g weight range, 9 (47.4%) were intubated in the delivery room, 6 due...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Continuous positive airway pressure ventilation; Preterm infants; Delivery room; Nasal continuous positive airway pressure; Prematurity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000892

Inhibition of cytohesin-1 by siRNA leads to reduced IGFR signaling in prostate cancer
Provedor de dados: 56
Autores: Weizhong,Zhang; Shuohui,Gao; Hanjiao,Qin; Yuhong,Man; Xiaohua,Yang; Jian,Cao; Lisen,Li.
To explore how cytohesin-1 (CYTH-1) small interfering RNA (siRNA) influences the insulin-like growth factor receptor (IGFR)-associated signal transduction in prostate cancer, we transfected human prostate cancer PC-3 cell lines with liposome-encapsulatedCYTH-1 siRNA in serum-free medium and exposed the cells to 100 nM IGF-1. The mRNA and protein levels of the signal molecules involved in the IGFR signaling pathways were determined by real-time PCR and detected by Western blotting. The relative mRNA levels of CYTH-1, c-Myc, cyclinD1 and IGF-1R (CYTH-1 siRNA group vs scrambled siRNA group) were 0.26 vs 0.97, 0.34 vs 1.06, 0.10 vs 0.95, and 0.27 vs 0.41 (P &lt; 0.05 for all), respectively. The relative protein levels of CYTH-1, pIGF-1R, pIRS1, pAkt1, pErk1,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytohesins; Insulin-like growth factor 1 receptor; Prostate cancer; Signal transduction; SiRNA interference.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700005

High-dose cyclophosphamide followed by autologous peripheral blood progenitor cell transplantation improves the salvage treatment for persistent or sensitive relapsed malignant lymphoma
Provedor de dados: 56
Autores: Baldissera,R.C.; Aranha,J.F.P.; Oliveira,G.B.; Vigorito,A.C.; Eid,K.A.B.; Miranda,E.C.M.; De Souza,C.A.
Trials have demonstrated that high-dose escalation followed by autologous transplantation can promote better long-term survival as salvage treatment in malignant lymphomas. The aim of the present nonrandomized clinical trial was to demonstrate the role of high-dose cyclophosphamide (HDCY) in reducing tumor burden and also to determine the effectiveness of HDCY followed by etoposide (VP-16) and methotrexate (MTX) in Hodgkin's disease plus high-dose therapy with peripheral blood progenitor cell (PBPC) transplantation as salvage treatment. From 1998 to 2000, 33 patients with a median age of 33 years (13-65) affected by aggressive non-Hodgkin's lymphoma (NHL) (60.6%) or persistent or relapsed Hodgkin's disease (39.4%) were enrolled and treated using high dose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malignant lymphomas; High-dose cyclophosphamide; Autologous peripheral blood progenitor cell transplantation; Salvage therapy.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100007

Influence of the dopaminergic system, CREB, and transcription factor-κB on cocaine neurotoxicity
Provedor de dados: 56
Autores: Planeta,C.S.; Lepsch,L.B.; Alves,R.; Scavone,C.
Cocaine is a widely used drug and its abuse is associated with physical, psychiatric and social problems. Abnormalities in newborns have been demonstrated to be due to the toxic effects of cocaine during fetal development. The mechanism by which cocaine causes neurological damage is complex and involves interactions of the drug with several neurotransmitter systems, such as the increase of extracellular levels of dopamine and free radicals, and modulation of transcription factors. The aim of this review was to evaluate the importance of the dopaminergic system and the participation of inflammatory signaling in cocaine neurotoxicity. Our study showed that cocaine activates the transcription factors NF-κB and CREB, which regulate genes involved in cellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocaine; Apoptosis; NF-κB; CREB; BDNF; Neurotoxicity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100909

Effect of bisacodyl on rats with slow transit constipation
Provedor de dados: 56
Autores: Wang,Yong-bing; Ling,Jie; Zhang,Wen-zhong; Li,Gang; Qiu,Wei; Zheng,Jun-hua; Zhao,Xiao-hui.
The effect of bisacodyl on the treatment of rats with slow transit constipation (STC) was studied. Forty-five female Wister rats were divided into control group, STC group, and STC bisacodyl group. The immunohistochemical method was used to determine interstitial cells of Cajal (ICC) and the expression of c-Kit protein. Body mass and the number of defecations were significantly decreased in the STC group compared with the control group on the 100th day after diphenoxylate administration, while dry weight of feces was significantly increased and the intestinal transit time was prolonged. There were significant differences in the number of defecations, dry weight of feces, and intestinal transit time among the three groups. The number of defecations was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bisacodyl; Slow transit constipation; C-Kit.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700613

Effects of acute administration of selective serotonin reuptake inhibitors on sympathetic nerve activity
Provedor de dados: 56
Autores: Tiradentes,R.V.; Pires,J.G.P.; Silva,N.F.; Ramage,A.G.; Santuzzi,C.H.; Futuro Neto,H.A.
Serotonergic mechanisms have an important function in the central control of circulation. Here, the acute effects of three selective serotonin (5-HT) reuptake inhibitors (SSRIs) on autonomic and cardiorespiratory variables were measured in rats. Although SSRIs require 2-3 weeks to achieve their full antidepressant effects, it has been shown that they cause an immediate inhibition of 5-HT reuptake. Seventy male Wistar rats were anesthetized with urethane and instrumented to record blood pressure, heart rate, renal sympathetic nerve activity (RSNA), and respiratory frequency. At lower doses, the acute cardiovascular effects of fluoxetine, paroxetine and sertraline administered intravenously were insignificant and variable. At middle and higher doses, a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoxetine; Paroxetine; Renal nerve activity; Serotonin; Sertraline; Sympathetic activity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700554

Molecular battles between plant and pathogenic bacteria in the phyllosphere
Provedor de dados: 56
Autores: Baker,C.M.; Chitrakar,R.; Obulareddy,N.; Panchal,S.; Williams,P.; Melotto,M.
The phyllosphere, i.e., the aerial parts of the plant, provides one of the most important niches for microbial colonization. This niche supports the survival and, often, proliferation of microbes such as fungi and bacteria with diverse lifestyles including epiphytes, saprophytes, and pathogens. Although most microbes may complete the life cycle on the leaf surface, pathogens must enter the leaf and multiply aggressively in the leaf interior. Natural surface openings, such as stomata, are important entry sites for bacteria. Stomata are known for their vital role in water transpiration and gas exchange between the plant and the environment that is essential for plant growth. Recent studies have shown that stomata can also play an active role in limiting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Guard cell; Plant innate immunity; Plant hormones; Bacterial pathogenesis; Food safety.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800001

Effects of a PPARG gene variant on obesity characteristics in Brazil
Provedor de dados: 56
Autores: Mattevi,V.S.; Zembrzuski,V.M.; Hutz,M.H.
The contribution of genetic factors to the development of obesity has been widely recognized, but the identity of the genes involved has not yet been fully clarified. Variation in genes involved in adipocyte differentiation and energy metabolism is expected to have a role in the etiology of obesity. We assessed the potential association of a polymorphism in one candidate gene, peroxisome proliferator-activated receptor-gamma (PPARGg), involved in these pathways and obesity-related phenotypes in 335 Brazilians of European descent. All individuals included in the sample were adults. Pregnant women, as well as those individuals with secondary hyperlipidemia due to renal, liver or thyroid disease, and diabetes, were not invited to participate in the study; all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; PPARG gene; Genetic susceptibility; Multifactorial diseases.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700006

Promoter region sequence differences in the A and G gamma globin genes of Brazilian sickle cell anemia patients
Provedor de dados: 56
Autores: Barbosa,C.G.; Goncalves-Santos,N.J.; Souza-Ribeiro,S.B.; Moura-Neto,J.P.; Takahashi,D.; Silva,D.O.; Hurtado-Guerrero,A.F.; Reis,M.G.; Goncalves,M.S.
Fetal hemoglobin (HbF), encoded by the HBG2 and HBG1 genes, is the best-known genetic modulator of sickle cell anemia, varying dramatically in concentration in the blood of these patients. This variation is partially associated with polymorphisms located in the promoter region of the HBG2 and HBG1 genes. In order to explore known and unknown polymorphisms in these genes, the sequences of their promoter regions were screened in sickle cell anemia patients and correlated with both their HbF levels and their βS-globin haplotypes. Additionally, the sequences were compared with genes from 2 healthy groups, a reference one (N = 104) and an Afro-descendant one (N = 98), to identify polymorphisms linked to the ethnic background.The reference group was composed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fetal hemoglobin; Sickle cell anemia; HBG1 gene; HBG2 gene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800002

Delayed Schwann cell and oligodendrocyte remyelination after ethidium bromide injection in the brainstem of Wistar rats submitted to streptozotocin diabetogenic treatment
Provedor de dados: 56
Autores: Bondan,E.F.; Lallo,M.A.; Trigueiro,A.H.; Ribeiro,C.P.; Sinhorini,I.L.; Graça,D.L.
Schwann cell disturbance followed by segmental demyelination in the peripheral nervous system occurs in diabetic patients. Since Schwann cell and oligodendrocyte remyelination in the central nervous system is a well-known event in the ethidium bromide (EB) demyelinating model, the aim of this investigation was to determine the behavior of both cell types after local EB injection into the brainstem of streptozotocin diabetic rats. Adult male Wistar rats received a single intravenous injection of streptozotocin (50 mg/kg) and were submitted 10 days later to a single injection of 10 µL 0.1% (w/v) EB or 0.9% saline solution into the cisterna pontis. Ten microliters of 0.1% EB was also injected into non-diabetic rats. The animals were anesthetized and perfused...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central nervous system; Diabetes mellitus; Ethidium bromide; Remyelination; Schwann cell; Oligodendrocyte.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500011

Long-term ethanol intoxication reduces inflammatory responses in rats
Provedor de dados: 56
Autores: Carvalho,E.M.; Brito,G.A.C.; Pessoa,B.B.G.P.; Ribeiro,R.A.; Capaz,F.R.
The anti-inflammatory effects of long-term ethanol intoxication were determined during ethanol treatment and withdrawal on the basis of neutrophil and eosinophil migration, hind paw edema and mast cell degranulation. Male Wistar rats (180-200 g, around 2 months of age) were exposed to increasing concentrations of ethanol vapor over a 10-day period. One group was evaluated immediately after exposure (treated group - intoxicated), and another was studied 7 h later (withdrawal group). Ethanol inhalation treatment significantly inhibited carrageenan- (62% for the intoxicated group, N = 5, and 35% for the withdrawal group, N = 6) and dextran-induced paw edema (32% for intoxicated rats and 26% for withdrawal rats, N = 5 per group). Ethanol inhalation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol; Inflammation; Carrageenan; Dextran; Air-pouch; Mast cells.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100013

Monoamines in the pedal plexus of the land snail Megalobulimus oblongus (Gastropoda, Pulmonata)
Provedor de dados: 56
Autores: Faccioni-Heuser,M.C.; Zancan,D.M.; Achaval,M.
In molluscs, the number of peripheral neurons far exceeds those found in the central nervous system. Although previous studies on the morphology of the peripheral nervous system exist, details of its organization remain unknown. Moreover, the foot of the terrestrial species has been studied less than that of the aquatic species. As this knowledge is essential for our experimental model, the pulmonate gastropod Megalobulimus oblongus, the aim of the present study was to investigate monoamines in the pedal plexus of this snail using two procedures: glyoxylic acid histofluorescence to identify monoaminergic structures, and the unlabeled antibody peroxidase anti-peroxidase method using antiserum to detect the serotonergic component of the plexus. Adult land...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pedal plexus; Catecholamines; Serotonin; Glyoxylic acid; Immunohistochemistry; Gastropod.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700014

Acute effect of different antidepressants on glycemia in diabetic and non-diabetic rats
Provedor de dados: 56
Autores: Gomez,R.; Huber,J.; Tombini,G.; Barros,H.M.T.
Diabetic patients have a 20% higher risk of depression than the general population. Treatment with antidepressant drugs can directly interfere with blood glucose levels or may interact with hypoglycemic agents. The treatment of depression in diabetic patients must take into account variations of glycemic levels at different times and a comparison of the available antidepressant agents is important. In the present study we evaluated the interference of antidepressants with blood glucose levels of diabetic and non-diabetic rats. In a first experiment, male adult Wistar rats were fasted for 12 h. Imipramine (5 mg/kg), moclobemide (30 mg/kg), clonazepam (0.25 mg/kg), fluoxetine (20 mg/kg) sertraline (30 mg/kg) or vehicle was administered. After 30 min, fasting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Depression; Antidepressants; Glucose tolerance; Diabetes; Glycemia.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100007

Effect of JJYMD-C, a novel synthetic derivative of gallic acid, on proliferation and phenotype maintenance in rabbit articular chondrocytes in vitro
Provedor de dados: 56
Autores: Xu,G.J.; Lu,Z.H.; Lin,X.; Lin,C.W.; Zheng,L.; Zhao,J.M.
Tissue engineering encapsulated cells such as chondrocytes in the carrier matrix have been widely used to repair cartilage defects. However, chondrocyte phenotype is easily lost when chondrocytes are expanded in vitro by a process defined as “dedifferentiation”. To ensure successful therapy, an effective pro-chondrogenic agent is necessary to overcome the obstacle of limited cell numbers in the restoration process, and dedifferentiation is a prerequisite. Gallic acid (GA) has been used in the treatment of arthritis, but its biocompatibility is inferior to that of other compounds. In this study, we modified GA by incorporating sulfamonomethoxine sodium and synthesized a sulfonamido-based gallate, JJYMD-C, and evaluated its effect on chondrocyte metabolism....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sulfamonomethoxine sodium; Gallic acid; Pro-chondrogenic agent; Chondrocyte; Rabbit articular cartilage; Dedifferentiation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800637

In silico analysis identifies a C3HC4-RING finger domain of a putative E3 ubiquitin-protein ligase located at the C-terminus of a polyglutamine-containing protein
Provedor de dados: 56
Autores: Scior,T.; Luna,F.; Koch,W.; Sánchez-Ruiz,J.F.
Almost identical polyglutamine-containing proteins with unknown structures have been found in human, mouse and rat genomes (GenBank AJ277365, AF525300, AY879229). We infer that an identical new gene (RING) finger domain of real interest is located in each C-terminal segment. A three-dimensional (3-D) model was generated by remote homology modeling and the functional implications are discussed. The model consists of 65 residues from terminal position 707 to 772 of the human protein with a total length of 796 residues. The 3-D model predicts a ubiquitin-protein ligase (E3) as a binding site for ubiquitin-conjugating enzyme (E2). Both enzymes are part of the ubiquitin pathway to label unwanted proteins for subsequent enzymatic degradation. The molecular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: E3 ubiquitin-protein ligase; RING finger structure; Low homology model.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300002

Protective effect of N-acetylcysteine against oxygen radical-mediated coronary artery injury
Provedor de dados: 56
Autores: Rodrigues,A.J.; Évora,P.R.B.; Schaff,H.V.
The present study investigated the protective effect of N-acetylcysteine (NAC) against oxygen radical-mediated coronary artery injury. Vascular contraction and relaxation were determined in canine coronary arteries immersed in Kreb's solution (95% O2-5% CO2), incubated or not with NAC (10 mM), and exposed to free radicals (FR) generated by xanthine oxidase (100 mU/ml) plus xanthine (0.1 mM). Rings not exposed to FR or NAC were used as controls. The arteries were contracted with 2.5 µM prostaglandin F2alpha. Subsequently, concentration-response curves for acetylcholine, calcium ionophore and sodium fluoride were obtained in the presence of 20 µM indomethacin. Concentration-response curves for bradykinin, calcium ionophore, sodium nitroprusside, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary arteries; N-acetylcysteine; Endothelium; Oxidative stress; Nitric oxide.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800012

Renal actions of angiotensin-(1-7)
Provedor de dados: 56
The heptapeptide angiotensin-(1-7) is considered to be a biologically active endproduct of the renin-angiotensin system. This angiotensin, which is devoid of the most known actions of angiotensin II such as induction of drinking behavior and vasoconstriction, has several selective effects in the brain and periphery. In the present article we briefly review recent evidence for a physiological role of angiotensin-(1-7) in the control of hydroelectrolyte balance
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Angiotensin antagonists; Angiotensin II; Hydroelectrolyte balance; Renin-angiotensin system; Kidney; Water transport.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400012

Overexpression of Fc receptor-like 1 associated with B-cell activation during hepatitis B virus infection
Provedor de dados: 56
Autores: Wang,Ke; Pei,Hao; Huang,Biao; Yang,Run-Lin; Wu,Hang-Yuan; Zhu,Xue; Zhu,Lan.
The role of B cells in the pathogenesis of hepatitis B virus (HBV) infection has not been explored in depth. In the present study, the activation status of B cells from peripheral blood of healthy controls (N = 20) and patients with acute hepatitis B (AHB, N = 15) or chronic hepatitis B (CHB, N = 30) was evaluated by measuring the expression levels of B-cell activation markers CD69 and CD86, using quantitative real-time PCR and flow cytometry. Moreover, the potential mechanism underlying B-cell activation during HBV infection was further investigated by analyzing the expression profile of FCRL1, an intrinsic activation molecule of B cells. An elevation in the levels of B-cell activation markers including CD69 and CD86 was observed in the AHB patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B virus; B-cell activation; Fc receptor-like 1; Gene expression.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200002

Obituary:José Moura Gonçalves (1914-1996)
Provedor de dados: 56
Autores: Lara,Francisco Jerônimo Salles.
Tipo: Info:eu-repo/semantics/other
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100001

Expression of neuronal nitric oxide synthase in the hippocampal formation in affective disorders
Provedor de dados: 56
Autores: Oliveira,R.M.W.; Guimarães,F.S.; Deakin,J.F.W.
Hippocampal output is increased in affective disorders and is mediated by increased glutamatergic input via N-methyl-D-aspartate (NMDA) receptor and moderated by antidepressant treatment. Activation of NMDA receptors by glutamate evokes the release of nitric oxide (NO) by the activation of neuronal nitric oxide synthase (nNOS). The human hippocampus contains a high density of NMDA receptors and nNOS-expressing neurons suggesting the existence of an NMDA-NO transduction pathway which can be involved in the pathogenesis of affective disorders. We tested the hypothesis that nNOS expression is increased in the human hippocampus from affectively ill patients. Immunocytochemistry was used to demonstrate nNOS-expressing neurons in sections obtained from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Affective disorders; Major depression; Neuronal nitric oxide synthase; Immunohistochemistry; Hippocampus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400012

Myosin V and iNOS expression is enhanced in J774 murine macrophages treated with IFN-gamma
Provedor de dados: 56
Autores: Reis,D.S.; Souza,M.A.; Mineo,J.R.; Espindola,F.S.
Actin-based motor protein requirements and nitric oxide (NO) production are important features of macrophage activity during phagocytosis or microbicidal processes. Different classes of myosins contribute directly or indirectly to phagocytosis by providing mechanical force for phagosome closure or organelle movement. Recent data have shown the presence of myosins IC, II, V and IXb in phagosomes of bone marrow-derived murine macrophages. In our investigation we demonstrated the presence of different classes of myosins in J774 macrophages. We also analyzed the effect of gamma interferon (IFN-gamma), with or without calcium ionophore or cytochalasin B, on myosins as well as on inducible nitric oxide synthase (iNOS) expression and NO production. Myosins IC,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myosins; Macrophages; IFN-gamma; INOS.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200009

Incidence of heart failure in infarcted rats that die spontaneously
Provedor de dados: 56
Autores: Flumignan,R.L.G.; Kanashiro,R.M.; Saraiva,R.M.; Portes,L.A.; Antonio,E.L.; Ishigai,M.M.S.; Tucci,P.J.F.
The present study reports for the first time the incidence of congestive heart failure (CHF) in previously infarcted rats that died spontaneously. Previously, pulmonary (PWC) and hepatic (HWC) water contents were determined in normal rats: 14 control animals were evaluated immediately after sacrifice, 8 placed in a refrigerator for 24 h, and 10 left at room temperature for 24 h. In the infarcted group, 9 rats died before (acute) and 28 died 48 h after (chronic) myocardial infarction. Thirteen chronic animals were submitted only to autopsy (N = 13), whereas PWC and HWC were also determined in the others (N = 15). Seven rats survived 48 h and died during anesthesia. Notably, PWC differed in normal rats: ambient (75.7 ± 1.3%) &lt; control (77.5 ± 0.7%) &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Myocardial infarction; Rats; Pulmonary congestion; Hepatic congestion.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001000008

Altered peripheral lymphocyte subsets in untreated systemic lupus erythematosus patients with infections
Provedor de dados: 56
Autores: Lu,Zhimin; Li,Jing; Ji,Juan; Gu,Zhifeng; Da,Zhanyun.
The leading cause of death in systemic lupus erythematosus (SLE) patients is infection. The objective of this study was to evaluate the distribution of lymphocyte subsets in untreated SLE patients with infections. This was a cross-sectional study. Data from January 2017 to May 2018 were collected. Flow cytometry was used to measure the peripheral lymphocyte subsets including CD3+T cells, CD4+T cells, CD8+T cells, CD19+B cells, CD3-CD16+CD56NK cells, and CD3+CD16+CD56NKT cells in 25 healthy controls and 52 treatment-naive SLE patients, among whom 13 were complicated with infections. Association between the lymphocyte subsets and infections was further analyzed. SLE patients with infections (n=13) showed a significantly higher incidence rate of fever (84.6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic lupus erythematosus; Lymphocyte subsets; Infection; C-reactive protein; Procalcitonin.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400609

Development of the insulin secretion mechanism in fetal and neonatal rat pancreatic B-cells: response to glucose, K+, theophylline, and carbamylcholine
Provedor de dados: 56
Autores: Mendonça,A.C.; Carneiro,E.M.; Bosqueiro,J.R.; Crepaldi-Alves,S.C.; Boschero,A.C.
We studied the development of the insulin secretion mechanism in the pancreas of fetal (19- and 21-day-old), neonatal (3-day-old), and adult (90-day-old) rats in response to stimulation with 8.3 or 16.7 mM glucose, 30 mM K+, 5 mM theophylline (Theo) and 200 µM carbamylcholine (Cch). No effect of glucose or high K+ was observed on the pancreas from 19-day-old fetuses, whereas Theo and Cch significantly increased insulin secretion at this age (82 and 127% above basal levels, respectively). High K+ also failed to alter the insulin secretion in the pancreas from 21-day-old fetuses, whereas 8.3 mM and 16.7 mM glucose significantly stimulated insulin release by 41 and 54% above basal levels, respectively. Similar results were obtained with Theo and Cch. A more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat islet cell; Glucose; Pancreatic islets; Carbamylcholine; Theophylline; Potassium; Insulin secretion.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600018

Heart rate variability in athletes and nonathletes at rest and during head-up tilt
Provedor de dados: 56
Autores: Martinelli,F.S.; Chacon-Mikahil,M.P.T.; Martins,L.E.B.; Lima-Filho,E.C.; Golfetti,R.; Paschoal,M.A.; Gallo-Junior,L.
The purpose of the present study was to determine if autonomic heart rate modulation, indicated by heart rate variability (HRV), differs during supine rest and head-up tilt (HUT) when sedentary and endurance-trained cyclists are compared. Eleven sedentary young men (S) and 10 trained cyclists (C) were studied. The volunteers were submitted to a dynamic ECG Holter to calculate HRV at rest and during a 70º HUT. The major aerobic capacity of athletes was expressed by higher values of &lt;img src="/img/revistas/bjmbr/v38n4/Vo.gif"&gt;at anaerobic threshold and peak conditions (P &lt; 0.05). At rest the athletes had lower heart rates (P &lt; 0.05) and higher values in the time domain of HRV compared with controls (SD of normal RR interval, SDNN, medians): 59.1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endurance; Power spectral analysis; Heart rate variability; Autonomic heart rate modulation; Training bradycardia; Orthostatic stress.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400019

The effect of porphyrins on normal and transformed mouse cell lines in the presence of visible light
Provedor de dados: 56
Autores: Tita,S.P.S; Perussi,J.R.
Photodynamic therapy consists of the uptake of a photosensitizing dye, often a porphyrin, by tumor tissue and subsequent irradiation of the tumor with visible light of an appropriate wavelength matched to the absorption spectrum of the photosensitizing dye. This class of molecules produces reactive oxygen species when activated by light, resulting in a direct or indirect cytotoxic effect on the target cells. Photodynamic therapy has been used in the treatment of cancer but the technology has a potential for the treatment of several disease conditions mainly because of its selectivity. However, it is not clear why the porphyrins are retained preferentially by abnormal tissue. This paper describes a study of the effect of the association of porphyrin and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water-soluble porphyrins; Mouse fibroblast lines A31 and B61; Photodynamic therapy; Visible light.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000014

Analysis of viral and cellular parameters which affect the fusion process of influenza viruses
Provedor de dados: 56
Autores: Barbosa,A.T.C.; Luiz,M.O.; Gusmão,N.P.; Couceiro,J.N.S.S.
In the present investigation we studied the fusogenic process developed by influenza A, B and C viruses on cell surfaces and different factors associated with virus and cell membrane structures. The biological activity of purified virus strains was evaluated in hemagglutination, sialidase and fusion assays. Hemolysis by influenza A, B and C viruses ranging from 77.4 to 97.2%, from 20.0 to 65.0%, from 0.2 to 93.7% and from 9.0 to 76.1% was observed when human, chicken, rabbit and monkey erythrocytes, respectively, were tested at pH 5.5. At this pH, low hemolysis indexes for influenza A, B and C viruses were observed if horse erythrocytes were used as target cells for the fusion process, which could be explained by an inefficient receptor binding activity of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Influenza A; B and C receptor binding activity sialidase activity fusion activity sialic acids cell surface charges.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200005

Vasopressor mechanisms in acute aortic coarctation hypertension
Provedor de dados: 56
Angiotensin II (ANG II) and vasopressin (AVP) act together with the mechanical effect of aortic constriction in the onset of acute aortic coarctation hypertension. Blockade of ANG II and AVP V1 receptors demonstrated that ANG II acts on the prompt (5 min) rise in pressure whereas AVP is responsible for the maintenance (30-45 min) of the arterial pressure elevation during aortic coarctation. Hormone assays carried out on blood collected from conscious rats submitted to aortic constriction supported a role for ANG II in the early stage and a combined role for both ANG II and AVP in the maintenance of proximal hypertension. As expected, a role for catecholamines was ruled out in this model of hypertension, presumably due to the inhibitory effect of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Aortic coarctation; Vasopressin; Angiotensin; Renal afferences; Hypertension.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400003

Apoptosis in parasites and parasite-induced apoptosis in the host immune system: a new approach to parasitic diseases
Provedor de dados: 56
Autores: Barcinski,M.A.; DosReis,G.A.
Apoptosis, a form of programmed cell death (PCD), has been described as essential for normal organogenesis and tissue development, as well as for the proper function of cell-renewal systems in adult organisms. Apoptosis is also pivotal in the pathogenesis of several different diseases. In this paper we discuss, from two different points of view, the role of apoptosis in parasitic diseases. The description of apoptotic death in three different species of heteroxenic trypanosomatids is reviewed, and considerations on the phylogenesis of apoptosis and on the eventual role of PCD on their mechanism of pathogenesis are made. From a different perspective, an increasing body of evidence is making clear that regulation of host cell apoptosis is an important factor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Parasites; Host cells; Host immune system; Programmed cell death; Trypanosomatids.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400003

Expression of NR1I3 in mouse lung tumors induced by the tobacco-specific nitrosamine 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone
Provedor de dados: 56
Autores: Fukumasu,H.; Cordeiro,Y.G.; Rochetti,A.L.; Barra,C.N.; Sámora,T.S.; Strefezzi,R.F.; Dagli,M.L.Z.
Nuclear receptor subfamily 1, group I, member 3 (NR1I3) is reported to be a possible novel therapeutic target for some cancers, including lung, brain and hematopoietic tumors. Here, we characterized expression of NR1I3 in a mouse model of lung carcinogenesis induced by 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone (NNK), the most potent tobacco carcinogen. Lung tumors were collected from mice treated with NNK (400 mg/kg) and euthanized after 52 weeks. Benign and malignant lesions were formalin-fixed and paraffin-embedded for histology and immunohistochemistry, with samples snap-frozen for mRNA analysis. Immunohistochemically, we found that most macrophages and type I and II pneumocytes expressed NR1I3, whereas fibroblasts and endothelial cells were...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: NR1I3; CAR; NNK; Cyclin D1; Lung carcinogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300240

Unusual association of NDM-1 with KPC-2 and armA among Brazilian Enterobacteriaceae isolates
Provedor de dados: 56
Autores: Quiles,M.G.; Rocchetti,T.T.; Fehlberg,L.C.; Kusano,E.J.U.; Chebabo,A.; Pereira,R.M.G.; Gales,A.C.; Pignatari,A.C.C.
We report the microbiological characterization of four New Delhi metallo-β-lactamase-1 (blaNDM-1)-producing Enterobacteriaceae isolated in Rio de Janeiro, Brazil. blaNDM-1 was located on a conjugative plasmid and was associated with Klebsiella pneumoniae carbapenemase-2 (blaKPC-2) or aminoglycoside-resistance methylase (armA), a 16S rRNA methylase not previously reported in Brazil, in two distinct strains of Enterobacter cloacae. Our results suggested that the introduction of blaNDM-1 in Brazil has been accompanied by rapid spread, since our isolates showed no genetic relationship.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Enterobacteriaceae; Carbapenem-resistance; Carbapenemases; Methylase.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200174

Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response
Provedor de dados: 56
Autores: Dang,N.N.; Pang,S.G.; Song,H.Y.; An,L.G.; Ma,X.L.
The objective of this study was to investigate whether a single defect in skin barrier function simulated by filaggrin silencing could induce Th2-predominant inflammation. Filaggrin gene expression was silenced in cultured normal human epidermal keratinocytes (NHEKs) using small hairpin RNA (shRNA, GTTGGCTCAAGCATATTATTT). The efficacy of silencing was confirmed by polymerase chain reaction (PCR) and Western blotting. Filaggrin-silenced cells (LV group), shRNA control cells (NC group), and noninfected cells (Blank group) were evaluated. The expression of cornified cell envelope-related proteins, including cytokeratin (CK)-5, -10, -14, loricrin, involucrin, and transglutaminase (TGM)-1, was detected by Western blotting. Interleukins (IL)-2, IL-4, IL-5,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atopic dermatitis; T helper 2 cytokines; Filaggrin silencing; Pathogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100039

Systemic injection of an H4 receptor agonist induces a decrease in CREB and pCREB levels in the cerebellar vermis and prefrontal cortex in mice
Provedor de dados: 56
Autores: Fernandes,C.E.M.; Serafim,K.R.; Gianlorenço,A.C.L.; Mattioli,R.
Studies have shown that an injection with the histamine H4 receptor agonist VUF-8430 modulates emotional memory processes. In the present study, the aim was to verify if intraperitoneal (ip) injection of VUF-8430 (500 ng/kg) in mice affects the synthesis of proteins required for memory consolidation processes by activating the phosphorylation of CREB (pCREB) in classical structures linked to emotional memory (prefrontal cortex, amygdala, and hippocampus) and the cerebellar vermis, a structure that has also been recently implicated in emotional memory. The results obtained using western blot analysis demonstrated that VUF-8430 induced a decrease in CREB and pCREB levels in the cerebellar vermis and prefrontal cortex, suggesting that this dose impaired the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CREB; PCREB; VUF-8430; Western blotting; Emotional memory; Mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500603

Thigmotactic responses in an open-field
Provedor de dados: 56
Autores: Lamprea,M.R.; Cardenas,F.P.; Setem,J; Morato,S.
The goal of the present study was to investigate the role of thigmotaxis (the tendency to remain close to vertical surfaces) in rat exploratory behavior in an open-field. Thigmotaxis was investigated in a parametric way, using 24 experimentally adult naive male Wistar rats (210-230 g). Exploratory behavior was studied in an open-field (N = 12) in 5-min sessions and behavior was analyzed in terms of where it occurred: in areas surrounded by two, one, or no walls. Another group of rats (N = 12) was studied in an open-field with blocks placed near two of the corners so as to make these corner areas surrounded by three walls. The floor of the open-fields was divided into 20-cm squares in order to locate the exact place of occurrence of each behavior. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exploratory behavior; Thigmotaxis; Open-field; Rearing behavior; Grooming; Locomotion.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200010

Interleukin-5 mediates peritoneal eosinophilia induced by the F1 cell wall fraction of Histoplasma capsulatum
Provedor de dados: 56
Autores: Sá-Nunes,A.; Medeiros,A.I.; Faccioli,L.H.
An alkali-insoluble fraction 1 (F1), which contains mainly ß-glucan isolated from the cell wall of Histoplasma capsulatum, induces eosinophil recruitment into the peritoneal cavity of mice. The present study was carried out to determine the participation of interleukin-5 (IL-5) in this process. Inbred C57BL/6 male mice weighing 15-20 g were treated ip with 100 µg of anti-IL-5 monoclonal antibody (TRFK-5, N = 7) or an isotype-matched antibody (N = 7), followed by 300 µg F1 in 1 ml PBS ip 24 h later. Controls (N = 5) received only 1 ml PBS. Two days later, cells from the peritoneal cavity were harvested by injection of 3 ml PBS and total cell counts were determined using diluting fluid in a Neubauer chamber. Differential counts were performed using...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Eosinophils; Histoplasma capsulatum; Inflammation; Interleukin-5; Anti-IL-5 monoclonal antibody.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300009

Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
Provedor de dados: 56
Autores: Mafra,R.A.; Leão,R.M.; Beirão,P.S.L.; Cruz,J.S.
A glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 ± 1.9 pA. Iglu was identified as a voltage-dependent inward current showing a strong rectification at positive potentials. L-Glutamate activated the inward current in a dose-dependent manner, with a half-maximal effect at about 18 µM and a maximum increase of 51.2 ± 4.4%. The inward current was blocked by the presence of dihydrokainate (0.5 mM), shown by others to readily block the GLT1 isoform. We thus speculate that Iglu could be attributed to the presence of a native glutamate transporter in cerebellar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebellar granule neurons; Excitatory amino-acid transporter type 2; Patch-clamp; Glutamate; Whole-cell voltage-clamp.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700018

Correlations of circulating peptide YY and ghrelin with body weight, rate of weight gain, and time required to achieve the recommended daily intake in preterm infants
Provedor de dados: 56
Autores: Chen,XiaFang; Du,Xueliang; Zhu,JianXing; Xie,LiJuan; Zhang,YongJun; He,ZhenJuan.
The objective was to elucidate the relationships between serum concentrations of the gut hormone peptide YY (PYY) and ghrelin and growth development in infants for potential application to the clinical observation index. Serum concentrations of PYY and ghrelin were measured using radioimmunoassay from samples collected at the clinic. For each patient, gestational age, birth weight, time required to return to birth weight, rate of weight gain, time required to achieve recommended daily intake (RDI) standards, time required for full-gastric feeding, duration of hospitalization, and time of administration of total parenteral nutrition were recorded. Serum PYY and ghrelin concentrations were significantly higher in the preterm group (N = 20) than in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peptide YY; Ghrelin; Preterm infants; Recommended daily intake.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700014

Use of the Wasserman equation in optimization of the duration of the power ramp in a cardiopulmonary exercise test: a study of Brazilian men
Provedor de dados: 56
Autores: Costa,D. C.; Santi,G. L. de; Crescêncio,J. C.; Seabra,L. P.; Carvalho,E. E. V.; Papa,V.; Marques,F.; Gallo Junior,L.; Schmidt,A.
This study aimed to analyze the agreement between measurements of unloaded oxygen uptake and peak oxygen uptake based on equations proposed by Wasserman and on real measurements directly obtained with the ergospirometry system. We performed an incremental cardiopulmonary exercise test (CPET), which was applied to two groups of sedentary male subjects: one apparently healthy group (HG, n=12) and the other had stable coronary artery disease (n=16). The mean age in the HG was 47±4 years and that in the coronary artery disease group (CG) was 57±8 years. Both groups performed CPET on a cycle ergometer with a ramp-type protocol at an intensity that was calculated according to the Wasserman equation. In the HG, there was no significant difference between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wasserman equation; Cardiopulmonary exercise test; Coronary artery disease; Cardiovascular diagnostic techniques.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201136

GABA-induced inactivation of Cebus apella V2 neurons: effects on orientation tuning and direction selectivity
Provedor de dados: 56
Autores: Jansen-Amorim,A.K.; Fiorani,M.; Gattass,R.
We investigated the GABA-induced inactivation of V2 neurons and terminals on the receptive field properties of this area in an anesthetized and paralyzedCebus apella monkey. Extracellular single-unit activity was recorded using tungsten microelectrodes in a monkey before and after pressure-injection of a 0.25 or 0.5 M GABA solution. The visual stimulus consisted of a bar moving in 8 possible directions. In total, 24 V2 neurons were studied before and after blocker injections in 4 experimental sessions following GABA injection into area V2. A group of 10 neurons were studied over a short period. An additional 6 neurons were investigated over a long period after the GABA injection. A third group of 8 neurons were studied over a very long period. Overall,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visual cortex; Single units; Extracellular recordings; New World monkeys.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700589

Acute effects of selective serotonin reuptake inhibitors on neuroleptic-induced catalepsy in mice
Provedor de dados: 56
Autores: Pires,J.G.P.; Bonikovski,V.; Futuro-Neto,H.A.
Depression found in Parkinson disease (PD) usually responds to selective serotonin reuptake inhibitors (SSRIs). Drugs that modify experimental neuroleptic catalepsy (NC) might affect extrapyramidal symptoms in PD. Therefore, the effects of SSRIs on NC were tested in mice, 26-36 g, separated by sex. Catalepsy was induced with haloperidol (H; 1 mg/kg, ip) and measured at 30-min intervals using a bar test. An SSRI (sertraline, ST; paroxetine, PX; fluoxetine) or vehicle (C) was injected ip 30 min before H. Dunnett's test was used for comparison of means. ST (1-5 mg/kg) or PX (1-5 mg/kg) attenuated NC, with a similar inhibition found in both sexes (5 mg/kg, 180 min: ST - males: 124 ± 10 vs 714 ± 15 s in C; females: 116 ± 10 vs 718 ± 6 s in C; PX - males: 106 ±...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Selective serotonin reuptake inhibitors; Fluoxetine; Sertraline; Paroxetine; Extrapyramidal system; Catalepsy.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200015

Transplantation of muscle-derived stem cells plus biodegradable fibrin glue restores the urethral sphincter in a pudendal nerve-transected rat model
Provedor de dados: 56
Autores: Xu,Y.; Song,Y.F.; Lin,Z.X.
We investigated whether fibrin glue (FG) could promote urethral sphincter restoration in muscle-derived stem cell (MDSC)-based injection therapies in a pudendal nerve-transected (PNT) rat, which was used as a stress urinary incontinence (SUI) model. MDSCs were purified from the gastrocnemius muscles of 4-week-old inbred female SPF Wistar rats and labeled with green fluorescent protein. Animals were divided into five groups (N = 15): sham (S), PNT (D), PNT+FG injection (F), PNT+MDSC injection (M), and PNT+MDSC+FG injection (FM). Each group was subdivided into 1- and 4-week groups. One and 4 weeks after injection into the proximal urethra, leak point pressure (LPP) was measured to assess urethral resistance function. Histology and immunohistochemistry were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscle-derived stem cells; Fibrin glue; Urinary incontinence; Cellular therapy; Pudendal nerve transection.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100009

Estrogen enhances vasoconstrictive remodeling after injury in male rabbits
Provedor de dados: 56
Autores: Francisco,Y.A.; Dantas,A.P.V.; Carvalho,M.H.C.; Laurindo,F.R.M.
The complete spectrum of estrogen vascular effects remains unclear. In particular, estrogen effects in the vascular response to profound injury in males have not been explored in detail. Therefore, we submitted 44 male New Zealand rabbits weighing 3.4 ± 0.6 kg to overdistention balloon injury of the right iliac artery. Rabbits were given 17ß-estradiol (5.45 µmol/day, sc) or vehicle for 7 days before and 14 days after injury, when the arteries were examined by post-mortem histomorphometry. Arteriographic caliber was assessed in vivo at baseline and before sacrifice. On day 14 after injury, in vivo arteriographic caliber (baseline = 2.44 ± 0.43 mm) was decreased by 23.1 ± 0.1% in controls and by 44.5 ± 0.1% in estrogen-treated rabbits (P &lt; 0.001). Neither...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Estrogen; Restenosis post-angioplasty; Nitric oxide; Endothelial function; Vascular remodeling.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900006

Antimicrobial resistance patterns of Haemophilus influenzae isolated from patients with meningitis in São Paulo, Brazil
Provedor de dados: 56
Autores: Casagrande,S.T.; Vicente,E.J.; Landgraf,I.M.; Kobata,A.M.M.
From 1989 to 1995, a total of 391 Haemophilus influenzae isolates were recovered from the cerebrospinal fluid (CSF) of hospitalized patients in São Paulo, Brazil. The majority of strains were isolated from infants aged less than 5 years. Strains belonging to biotype I (64.7%), biotype II (34.5%) and biotype IV (0.76%) were detected. Ninety-nine percent of these strains were serotype b. Minimal inhibitory concentration (MIC) was determined for ampicillin, chloramphenicol and ceftriaxone. The ß-lactamase assay was performed for all strains. The rate of ß-lactamase producer strains ranged from 10 to 21.4% during a period of 7 years, with an overall rate of 13.8%. Of the 391 strains analyzed, none was ß-lactamase negative ampicillin resistant (BLNAR). A total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haemophilus influenzae; Meningitis; Antimicrobial resistance.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300006

Anxiolytic-like effects of 4-phenyl-2-trichloromethyl-3H-1,5-benzodiazepine hydrogen sulfate in mice
Provedor de dados: 56
Autores: Rubin,M.A.; Albach,C.A.; Berlese,D.B.; Bonacorso,H.G.; Bittencourt,S.R.T.; Queiroz,C.M.T.; Maixner,A.E.; Mello,C.F.
The pharmacological effects of 4-phenyl-2-trichloromethyl-3H-1,5-benzodiazepine hydrogen sulfate (PTMB), a novel synthetic benzodiazepine, were examined in mice. In the elevated plus-maze test of anxiety, 0.3-1 mg/kg diazepam ip (F(3,53) = 3.78; P&lt;0.05) and 1-10 mg/kg PTMB ip increased (F(5,98) = 3.26; P&lt;0.01), whereas 2 mg/kg picrotoxin ip decreased (F(3,59) = 8.32; P&lt;0.001) the proportion of time spent in the open arms, consistent with an anxiolytic action of both benzodiazepines, and an anxiogenic role for picrotoxin. In the holeboard, 1.0 mg/kg diazepam ip increased (F(3,54) = 2.78; P&lt;0.05) and 2 mg/kg picrotoxin ip decreased (F(3,59) = 4.69; P&lt;0.01) locomotor activity. Rotarod assessment revealed that 1 mg/kg diazepam ip and 3, 10 and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Anxiolytic 1; 5-benzodiazepine diazepam elevated plus-maze holeboard picrotoxin.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900013

Differences in prostate cancer detection between Canadian and Saudi populations
Provedor de dados: 56
Autores: Al-Abdin,O.Z.; Rabah,D.M.; Badr,G.; Kotb,A.; Aprikian,A.
Few studies have addressed racial differences in prostate cancer (PCa) detection between Western and Arabian countries, although PCa has a significantly lower prevalence in Arabic populations compared to Western populations. Therefore, an explanation of this difference is lacking. Serum prostate-specific antigen (PSA) is a valuable marker used to select patients who should undergo prostate biopsies, although the manner in which it is used may require adjustments based on the ethnic population in question. We investigated racial differences in the PCa detection rate between Canadian and Saudi populations. A retrospective analysis was performed of data collected prospectively over 5 consecutive years in urology clinics at the McGill University Health Center...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; Serum; PSA; PSA density; Digital rectal examination.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600539

Control of CD4 gene expression: connecting signals to outcomes in T cell development
Provedor de dados: 56
Autores: Sarafova,S.D.; Siu,G.
The control of CD4 gene expression is essential for proper T lymphocyte development. Signals transmitted from the T-cell antigen receptor (TCR) during the thymic selection processes are believed to be linked to the regulation of CD4 gene expression during specific stages of T cell development. Thus, a study of the factors that control CD4 gene expression may lead to further insight into the molecular mechanisms that drive thymic selection. In this review, we discuss the work conducted to date to identify and characterize the cis-acting transcriptional control elements in the CD4 locus and the DNA-binding factors that mediate their function. From these studies, it is becoming clear that the molecular mechanisms controlling CD4 gene expression are very...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CD4; Gene expression; Silencer; T cell; Development.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700001

Probing the SERCA1a sarcoplasmic reticulum Ca2+-ATPase phosphorylation-site mutant D351E with inorganic phosphate
Provedor de dados: 56
Autores: Carreira,A.C.O.; Bastos,C.M.V.; Verjovski-Almeida,S.
The expression of sarcoplasmic reticulum SERCA1a Ca2+-ATPase wild-type and D351E mutants was optimized in yeast under the control of a galactose promoter. Fully active wild-type enzyme was recovered in yeast microsomal membrane fractions in sufficient amounts to permit a rapid and practical assay of ATP hydrolysis and phosphoenzyme formation from ATP or Pi. Mutant and wild-type Ca2+-ATPase were assayed for phosphorylation by Pi under conditions that are known to facilitate this reaction in the wild-type enzyme, including pH 6.0 or 7.0 at 25ºC in the presence of dimethylsulfoxide. Although glutamyl (E) and aspartyl (D) residue side chains differ by only one methylene group, no phosphoenzyme could be detected in the D351E mutant, even upon the addition of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phosphorylation site mutant; Catalytic site; Yeast expression system; Saccharomyces cerevisiae; P-type ATPase.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000004

Interaction between paraventricular nucleus and septal area in the control of physiological responses induced by angiotensin II
Provedor de dados: 56
Autores: Camargo,L.A.A.; Saad,W.A.; Simões,S.; Santos,T.A.B.; Saad,W. Abrão.
We determined the effects of losartan (40 nmol) and PD 123319 (40 nmol) (both non-peptides and selective antagonists of the AT1 and AT2 angiotensin receptors, respectively), and [Sar¹, Ala8] angiotensin II (ANG II) (40 nmol) (a non-selective peptide antagonist of angiotensin receptors) injected into the paraventricular nucleus (PVN) on the water and salt appetite, diuresis and natriuresis and mean arterial pressure (MAP) induced by administration of 10 nmol of ANG II into the medial septal area (MSA) of male Holtzman rats weighing 250-300 g. The volume of drug solution injected was 0.5 µl over a period of 10-15 s. The responses were measured over a period of 120 min. ANG II alone injected into the MSA induced an increase in all the above parameters (8.1 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AT1 receptors; AT2 receptors; Water; Sodium; Paraventricular nucleus; Medial septal area.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900002

Molecular characterization of DDX26, a human DEAD-box RNA helicase, located on chromosome 7p12
Provedor de dados: 56
Autores: Camargo,A.A.; Nunes,D.N.; Samaia,H.B.; Liu,L.; Collins,V.P.; Simpson,A.J.G.; Dias-Neto,E.
DEAD-box proteins comprise a family of ATP-dependent RNA helicases involved in several aspects of RNA metabolism. Here we report the characterization of the human DEAD-box RNA helicase DDX26. The gene is composed of 14 exons distributed over an extension of 8,123 bp of genomic sequence and encodes a transcript of 1.8 kb that is expressed in all tissues evaluated. The predicted amino acid sequence shows a high similarity to a yeast DEAD-box RNA helicase (Dbp9b) involved in ribosome biogenesis. The new helicase maps to 7p12, a region of frequent chromosome amplifications in glioblastomas involving the epidermal growth factor receptor (EGFR) gene. Nevertheless, co-amplification of DDX26 with EGFR was not detected in nine tumors analyzed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DDX26; DEAD-box RNA helicase; 7p12; Ribosome biogenesis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000002

Genetic variability of vascular endothelial growth factor and prognosis of head and neck cancer in a Brazilian population
Provedor de dados: 56
Autores: Ruiz,M.T.; Biselli,P.M.; Maniglia,J.V.; Pavarino-Bertelli,E.C.; Goloni-Bertollo,E.M.
Vascular endothelial growth factor (VEGF) is one of the most potent endothelial cell mitogens and plays a critical role in angiogenesis. Polymorphisms in this gene have been evaluated in patients with several types of cancer. The objectives of this study were to determine if there was an association of the -1154G/A polymorphism of the VEGF gene with head and neck cancer and the interaction of this polymorphism with lifestyle and demographic factors. Additionally, the distribution of the VEGF genotype was investigated with respect to the clinicopathological features of head and neck cancer patients. The study included 100 patients with histopathological diagnosis of head and neck squamous cell carcinoma. Patients with treated tumors were excluded. A total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Head and neck cancer; Polymorphism; Genetic; VEGF gene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200002

Immune response induced in mice oral immunization with cowpea severe mosaic virus
Provedor de dados: 56
Autores: Florindo,M.I.; Aragão,M.E.F. de; Silva,A.C.M. da; Otoch,M.L.; Melo,D. Fernandes de; Lima,J.A.A.; Lima,M.G. Silva.
There is increasing interest in the immune response induced by plant viruses since these could be used as antigen-expressing systems in vaccination procedures. Cowpea severe mosaic virus (CPSMV), as a purified preparation (300 g of leaves, 2 weeks post-inoculation), or crude extract from cowpea (Vigna unguiculata) leaves infected with CPSMV both administered by gavage to Swiss mice induced a humoral immune response. Groups of 10 Swiss mice (2-month-old females) were immunized orally with 10 daily doses of either 50 µg viral capsid protein (boosters of 50 µg at days 21 and 35 after immunization) or 0.6 mg protein of the crude extract (boosters of 0.6 mg at days 21 and 35 after immunization). Anti-CPSMV antibodies were quantified by ELISA in pooled sera...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral immunization; Plant virus; Plant virus immunogenicity; Cowpea severe mosaic virus.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700011

Determination of multidrug resistance mechanisms in Clostridium perfringens type A isolates using RNA sequencing and 2D-electrophoresis
Provedor de dados: 56
Autores: Ma,Yu-Hua; Ye,Gui-Sheng.
In this study, we screened differentially expressed genes in a multidrug-resistant isolate strain of Clostridium perfringens by RNA sequencing. We also separated and identified differentially expressed proteins (DEPs) in the isolate strain by two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). The RNA sequencing results showed that, compared with the control strain, 1128 genes were differentially expressed in the isolate strain, and these included 227 up-regulated genes and 901 down-regulated genes. Bioinformatics analysis identified the following genes and gene categories that are potentially involved in multidrug resistance (MDR) in the isolate strain: drug transport, drug response, hydrolase activity, transmembrane transporter,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Multidrug resistance; RNA sequencing; 2D-electrophoresis; Molecular mechanism.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800609

Positive correlation of serum leptin with estradiol levels in patients with polycystic ovary syndrome
Provedor de dados: 56
Autores: Mendonça,H.C.; Montenegro Junior,R.M.; Foss,M.C.; Silva de Sá,M.F.; Ferriani,R.A.
Patients with polycystic ovary syndrome (PCOS) usually are obese, insulin resistant and hyperinsulinemic. The known association between leptin, obesity andinsulin action suggests that leptin may have a role in PCOS but this has only been addressed peripherally. This study was designed to assess the relationship between serum leptin and the anthropometric, metabolic and endocrine variables of obese (body mass index, BMI ³30 kg/m²) and non-obese (BMI &lt;30 kg/m²) PCOS patients. Twenty-eight PCOS patients and 24 control women subdivided into obese and non-obese groups were evaluated. Leptin, androgens, lipids, gonadotrophins and insulin-glucose response to the oral glucose tolerance test were measured by radioimmunoassay in all participants. The assays were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptin; Polycystic ovary syndrome; Insulin; Insulin sensitivity; Estrogen.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500015

Biological evaluation of recombinant human erythropoietin in pharmaceutical products
Provedor de dados: 56
Autores: Ramos,A.S.; Schmidt,C.A.; Andrade,S.S.; Fronza,M.; Rafferty,B.; Dalmora,S.L.
The potencies of mammalian cell-derived recombinant human erythropoietin pharmaceutical preparations, from a total of five manufacturers, were assessed by in vivo bioassay using standardized protocols. Eight-week-old normocythemic mice received a single subcutaneous injection followed by blood sampling 96 h later or multiple daily injections with blood sampling 24 h after the last injection. Reticulocyte counting by microscopic examination was employed as the end-point using the brilliant cresyl blue or selective hemolysis methods, together with automated flow cytometry. Different injection schedules were investigated and dose-response curves for the European Pharmacopoeia Biological Reference Preparation of erythropoietin were compared. Manual and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recombinant human erythropoietin; Bioassay; Polycythemia; Reticulocyte counting; Normocythemic mice.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100014

Dopaminergic modulation of grooming behavior in virgin and pregnant rats
Provedor de dados: 56
Autores: Serafim,A.P.; Felicio,L.F.
Dopamine receptors are involved in the expression of grooming behavior. The pregnancy-induced increase in self-licking observed in rats is important for mammary gland development and lactation. This study focuses on the role of dopamine receptor subtypes in grooming behavior of virgin and pregnant female rats. General and mammary gland grooming were measured in virgin rats treated with 0.25 mg/kg of the D1-like agonist SKF-81297 and antagonist SKF-83566 and the D2-like agonist lisuride and antagonist sulpiride. The effects of 0.01 and 0.25 mg/kg doses of the same agonists and antagonists were evaluated in pregnant rats as well. In virgin animals both SKF-83566 and sulpiride treatments significantly reduced the time spent in general grooming, while none of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Self-licking; Lisuride; SKF-81297; SKF-83566; Sulpiride; Mammary gland; Prolactin; Dopamine.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100015

Vascular O-GlcNAcylation augments reactivity to constrictor stimuli by prolonging phosphorylated levels of the myosin light chain
Provedor de dados: 56
Autores: Lima,V.V.; Lobato,N.S.; Filgueira,F.P.; Webb,R.C.; Tostes,R.C.; Giachini,F.R.
O-GlcNAcylation is a modification that alters the function of numerous proteins. We hypothesized that augmented O-GlcNAcylation levels enhance myosin light chain kinase (MLCK) and reduce myosin light chain phosphatase (MLCP) activity, leading to increased vascular contractile responsiveness. The vascular responses were measured by isometric force displacement. Thoracic aorta and vascular smooth muscle cells (VSMCs) from rats were incubated with vehicle or with PugNAc, which increases O-GlcNAcylation. In addition, we determined whether proteins that play an important role in the regulation of MLCK and MLCP activity are directly affected by O-GlcNAcylation. PugNAc enhanced phenylephrine (PE) responses in rat aortas (maximal effect, 14.2±2 vs 7.9±1 mN for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: O-GlcNAcylation; Myosin light chain; Vascular reactivity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000826

Genetic analysis of the porcine group B rotavirus NSP2 gene from wild-type Brazilian strains
Provedor de dados: 56
Autores: Médici,K.C.; Barry,A.F.; Alfieri,A.F.; Alfieri,A.A.
Group B rotaviruses (RV-B) were first identified in piglet feces, being later associated with diarrhea in humans, cattle, lambs, and rats. In human beings, the virus was only described in China, India, and Bangladesh, especially infecting adults. Only a few studies concerning molecular analysis of the RV-B NSP2 gene have been conducted, and porcine RV-B has not been characterized. In the present study, three porcine wild-type RV-B strains from piglet stool samples collected from Brazilian pig herds were used for analysis. PAGE results were inconclusive for those samples, but specific amplicons of the RV-B NSP2 gene (segment 8) were obtained in a semi-nested PCR assay. The three porcine RV-B strains showed the highest nucleotide identity with the human WH1...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Piglets; Diarrhea; Group B rotavirus; NSP2 gene; Phylogenetic analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100003

Association of body mass index with disease severity and prognosis in patients with non-cystic fibrosis bronchiectasis
Provedor de dados: 56
Autores: Qi,Q.; Li,T.; Li,J.C.; Li,Y.
The objective of this observational, multicenter study was to evaluate the association of body mass index (BMI) with disease severity and prognosis in patients with non-cystic fibrosis bronchiectasis. A total of 339 patients (197 females, 142 males) diagnosed with non-cystic fibrosis bronchiectasis by high-resolution computed tomography were classified into four groups: underweight (BMI&lt;18.5 kg/m2), normal weight (18.5≤BMI&lt;25.0 kg/m2), overweight (25.0≤BMI&lt;30.0 kg/m2), and obese (BMI≥30.0 kg/m2). Clinical variables expressing disease severity were recorded, and acute exacerbations, hospitalizations, and survival rates were estimated during the follow-up period. The mean BMI was 21.90 kg/m2. The underweight group comprised 28.61% of all patients....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bronchiectasis; Body mass index; Prognosis; Survival; Underweight.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800715

Adhesion of the human pathogen Sporothrix schenckii to several extracellular matrix proteins
Provedor de dados: 56
Autores: Lima,O.C.; Figueiredo,C.C.; Pereira,B.A.S.; Coelho,M.G.P.; Morandi,V.; Lopes-Bezerra,L.M.
The pathogenic fungus Sporothrix schenckii is the causative agent of sporotrichosis. This subcutaneous mycosis may disseminate in immunocompromised individuals and also affect several internal organs and tissues, most commonly the bone, joints and lung. Since adhesion is the first step involved with the dissemination of pathogens in the host, we have studied the interaction between S. schenckii and several extracellular matrix (ECM) proteins. The binding of two morphological phases of S. schenckii, yeast cells and conidia, to immobilized type II collagen, laminin, fibronectin, fibrinogen and thrombospondin was investigated. Poly (2-hydroxyethyl methacrylate) (poly-HEMA) was used as the negative control. Cell adhesion was assessed by ELISA with a rabbit...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sporothrix schenckii; Fibronectin; Laminin; Type II collagen; Adhesion.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500020

Prenatally programmed hypertension: role of maternal diabetes
Provedor de dados: 56
Autores: Gomes,G.N.; Gil,F.Z.
Epidemiological and experimental studies have led to the hypothesis of the fetal origin of adult diseases, suggesting that some adult diseases might be determined before birth by altered fetal development. Maternal diabetes subjects the fetus to an adverse environment that has been demonstrated to result in metabolic, cardiovascular and renal impairment in the offspring. The growing amount of obesity in young females in developed and some developing countries should contribute to increasing the incidence of diabetes among pregnant women. In this review, we discuss how renal and extrarenal mechanisms participate in the genesis of hypertension induced by a diabetic status during fetal development.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Maternal diabetes; Renal impairment; Sodium retention.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900011

Cardiorespiratory fitness, pulmonary function and C-reactive protein levels in nonsmoking individuals with diabetes
Provedor de dados: 56
Autores: Francisco,C.O.; Catai,A.M.; Moura-Tonello,S.C.G.; Lopes,S.L.B.; Benze,B.G.; Del Vale,A.M.; Leal,A.M.O.
The objective of this study was to evaluate cardiorespiratory fitness and pulmonary function and the relationship with metabolic variables and C-reactive protein (CRP) plasma levels in individuals with diabetes mellitus (DM). Nineteen men with diabetes and 19 age- and gender-matched control subjects were studied. All individuals were given incremental cardiopulmonary exercise and pulmonary function tests. In the exercise test, maximal workload (158.3±22.3vs 135.1±25.2, P=0.005), peak heart rate (HRpeak: 149±12 vs 139±10, P=0.009), peak oxygen uptake (VO2peak: 24.2±3.2 vs18.9±2.8, P&lt;0.001), and anaerobic threshold (VO2VT: 14.1±3.4 vs 12.2±2.2, P=0.04) were significantly lower in individuals with diabetes than in control subjects. Pulmonary function test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Pulmonary function test; Cardiopulmonary exercise test; Glycemic control; C-reactive protein.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500426

The modulatory effect of estradiol benzoate on superoxide dismutase activity in the developing rat brain
Provedor de dados: 56
Autores: Pejic,S.; Kasapovic,J.; Cvetkovic,D.; Pajovic,S.B.
The sensitivity of copper,zinc (CuZn)- and manganese (Mn)-superoxide dismutase (SOD) to exogenous estradiol benzoate (EB) was investigated in Wistar rats during postnatal brain development. Enzyme activities were measured in samples prepared from brains of rats of both sexes and various ages between 0 and 75 days, treated sc with 0.5 µg EB/100 g body weight in 0.1 ml olive oil/100 g body weight, 48 and 24 h before sacrifice. In females, EB treatment stimulated MnSOD activity on days 0 (66.1%), 8 (72.7%) and 15 (81.7%). In males, the stimulatory effect of EB on MnSOD activity on day 0 (113.6%) disappeared on day 8 and on days 15 and 45 it became inhibitory (40.3 and 30.5%, respectively). EB had no effect on the other age groups. The stimulatory effect of EB...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain; Brain development; Estradiol; Superoxide dismutase.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500004

HLA-DRB1 alleles in juvenile-onset systemic lupus erythematosus: renal histologic class correlations
Provedor de dados: 56
Autores: Liphaus,B.L.; Kiss,M.H.B.; Goldberg,A.C.
Human leukocyte antigens (HLA) DRB1*03 and DRB1*02 have been associated with systemic lupus erythematosus (SLE) in Caucasians and black populations. It has been observed that certain HLA alleles show stronger associations with SLE autoantibodies and clinical subsets, although they have rarely been associated with lupus renal histologic class. In the present study, HLA-DRB1 allele correlations with clinical features, autoantibodies and renal histologic class were analyzed in a cohort of racially mixed Brazilian patients with juvenile-onset SLE. HLA-DRB1 typing was carried out by polymerase chain reaction amplification with sequence-specific primers using genomic DNA from 55 children and adolescents fulfilling at least four of the American College of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic lupus erythematosus; Human leukocyte antigen; Lupus nephritis; Juvenile-onset lupus; HLA-DRB1 alleles.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400019

Uptake of NO-releasing drugs by the P2 nucleoside transporter in trypanosomes
Provedor de dados: 56
Autores: Soulère,L.; Hoffmann,P.; Bringaud,F.; Périé,J.
Nitric oxide (NO·) has been identified as a principal regulatory molecule of the immune system and the major cytotoxic mediator of activated immune cells. NO· can also react rapidly with a variety of biological species, particularly with the superoxide radical anion O2·- at almost diffusion-limited rates to form peroxynitrite anion (ONOO-). ONOO- and its proton-catalyzed decomposition products are capable of oxidizing a great diversity of biomolecules and can act as a source of toxic hydroxyl radicals. As a consequence, a strategy for the development of molecules with potential trypanocidal activities could be developed to increase the concentration of nitric oxide in the parasites through NO·-releasing compounds. In this way, the rate of formation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Peroxynitrite; Thionitrite; S-nitrosothiol; Adenosine.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100016

Comparison of voluntary ethanol intake by two pairs of rat lines used as genetic models of anxiety
Provedor de dados: 56
Autores: Da Silva,G.E.; Ramos,A.; Takahashi,R.N.
The relationship between anxiety-related behaviors and voluntary ethanol intake was examined in two pairs of rat lines by the oral ethanol self-administration procedure. Floripa high (H) and low (L) rats selectively bred for contrasting anxiety responses in the open-field test, and two inbred strains, spontaneously hypertensive rats (SHR) and Lewis rats which are known to differ significantly when submitted to several behavioral tests of anxiety/emotionality, were used (9-10 animals/line/sex). No differences in the choice of ethanol solutions (2%, days 1-4, and 4%, days 5-8, respectively) in a 2-bottle paradigm were detected between Floripa H and L rats (1.94 ± 0.37 vs 1.61 ± 0.37 g/kg for ethanol intake on day 8 by the Floripa H and L rat lines,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anxiety; Ethanol intake; Spontaneously hypertensive rats; Lewis; Selected lines; Inbred strains.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000010

Papers from the Ribeirão Preto and São Luís birth cohort studies: presentation
Provedor de dados: 56
Autores: Barbieri,Marco Antonio; Bettiol,Heloisa; Silva,Antônio Augusto Moura da; Goldani,Marcelo Zubaran.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900002

Delivery of the Sox9 gene promotes chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells in an in vitro model
Provedor de dados: 56
Autores: Wang,Z.H.; Li,X.L.; He,X.J.; Wu,B.J.; Xu,M.; Chang,H.M.; Zhang,X.H.; Xing,Z.; Jing,X.H.; Kong,D.M.; Kou,X.H.; Yang,Y.Y.
SRY-related high-mobility-group box 9 (Sox9) gene is a cartilage-specific transcription factor that plays essential roles in chondrocyte differentiation and cartilage formation. The aim of this study was to investigate the feasibility of genetic delivery of Sox9 to enhance chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells (hUC-MSCs). After they were isolated from human umbilical cord blood within 24 h after delivery of neonates, hUC-MSCs were untreated or transfected with a human Sox9-expressing plasmid or an empty vector. The cells were assessed for morphology and chondrogenic differentiation. The isolated cells with a fibroblast-like morphology in monolayer culture were positive for the MSC markers CD44, CD105,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic modification; Tissue engineering; Stem cells; Sox9; Chondrogenesis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400279

Effect of estradiol and bisphenol A on human hepatoblastoma cell viability and telomerase activity
Provedor de dados: 56
Autores: Xu,B.L.; Zhao,Q.Z.; Gao,X.Y.; Hou,G.J.
Sex hormones from environmental and physiological sources might play a major role in the pathogenesis of hepatoblastoma in children. This study investigated the effects of estradiol and bisphenol A on the proliferation and telomerase activity of human hepatoblastoma HepG2 cells. The cells were divided into 6 treatment groups: control, bisphenol A, estradiol, anti-estrogen ICI 182,780 (hereinafter ICI), bisphenol A+ICI, and estradiol+ICI. Cell proliferation was measured based on average absorbance using the Cell Counting-8 assay. The cell cycle distribution and apoptotic index were determined by flow cytometry. Telomerase activity was detected by polymerase chain reaction and a telomeric repeat amplification protocol assay. A higher cell density was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estradiol; Bisphenol A; HepG2; Telomerase activity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101004

Tumor growth reduction is regulated at the gene level in Walker 256 tumor-bearing rats supplemented with fish oil rich in EPA and DHA
Provedor de dados: 56
Autores: Borghetti,G.; Yamazaki,R.K.; Coelho,I.; Pequito,D.C.T.; Schiessel,D.L.; Kryczyk,M.; Mamus,R.; Naliwaiko,K.; Fernandes,L.C.
We investigated the effect of fish oil (FO) supplementation on tumor growth, cyclooxygenase 2 (COX-2), peroxisome proliferator-activated receptor gamma (PPARγ), and RelA gene and protein expression in Walker 256 tumor-bearing rats. Male Wistar rats (70 days old) were fed with regular chow (group W) or chow supplemented with 1 g/kg body weight FO daily (group WFO) until they reached 100 days of age. Both groups were then inoculated with a suspension of Walker 256 ascitic tumor cells (3×107 cells/mL). After 14 days the rats were killed, total RNA was isolated from the tumor tissue, and relative mRNA expression was measured using the 2-ΔΔCT method. FO significantly decreased tumor growth (W=13.18±1.58 vsWFO=5.40±0.88 g, P&lt;0.05). FO supplementation also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Fish oil; Gene expression.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800696

Changes in protein profile during coagulation of latex from Carica papaya
Provedor de dados: 56
We describe the changes in peptide composition by SDS-PAGE analysis of latex from Carica papaya collected at various times after incision of the unripe fruit. The data show that during latex coagulation several peptides are processed in an orderly fashion.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Carica papaya; Latex; Endopeptidase; Cysteine proteinase; Papain; Coagulation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500007

Protective effects of centrally acting sympathomodulatory drugs on myocardial ischemia induced by sympathetic overactivity in rabbits
Provedor de dados: 56
Autores: Catelli,M.; Feldman,J.; Bousquet,P.; Tibiriçá,E.
It is recognized that an imbalance of the autonomic nervous system is involved in the genesis of ventricular arrhythmia and sudden death during myocardial ischemia. In the present study we investigated the effects of clonidine and rilmenidine, two centrally acting sympathomodulatory drugs, on an experimental model of centrally induced sympathetic hyperactivity in pentobarbital-anesthetized New Zealand albino rabbits of either sex (2-3 kg, N = 89). We also compared the effects of clonidine and rilmenidine with those of propranolol, a ß-blocker, known to induce protective cardiovascular effects in patients with ischemic heart disease. Central sympathetic stimulation was achieved by intracerebroventricular injection of the excitatory amino acid L-glutamate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autonomic nervous system; Antihypertensive agents; Antiarrhythmic agents; Ischemia; Sudden death.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100012

Participation of the cholinergic system in the ethanol-induced suppression of paradoxical sleep in rats
Provedor de dados: 56
Autores: Papale,L.A.; Andersen,M.L.; Perry,J.C.; Tufik,S.
Sleep disturbance is among the many consequences of ethanol abuse in both humans and rodents. Ethanol consumption can reduce REM or paradoxical sleep (PS) in humans and rats, respectively. The first aim of this study was to develop an animal model of ethanol-induced PS suppression. This model administered intragastrically (by gavage) to male Wistar rats (3 months old, 200-250 g) 0.5 to 3.5 g/kg ethanol. The 3.5 g/kg dose of ethanol suppressed the PS stage compared with the vehicle group (distilled water) during the first 2-h interval (0-2 h; 1.3 vs 10.2; P &lt; 0.001). The second aim of this study was to investigate the mechanisms by which ethanol suppresses PS. We examined the effects of cholinergic drug pretreatment. The cholinergic system was chosen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholinergic; Atropine; Pilocarpine; Ethanol; Paradoxical sleep; Electroencephalography.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900007

Publication in a Brazilian journal by Brazilian scientists whose papers have international impact
Provedor de dados: 56
Autores: Meneghini,R.
Nine Brazilian scientists with an outstanding profile of international publications were invited to publish an original article in the same issue of a Brazilian Journal (Anais da Academia Brasileira de Ciências). The objective was to measure the impact of the papers on the number of citations to the articles, the assumption being that these authors would carry their international prestige to the Brazilian periodical. In a 2-year period there was a larger number of citations of these articles compared to others published in the same journal. Nevertheless, the number of citations in Brazilian journals did not equal the number of citations obtained by the other papers by the same authors in their international publications within the same 2-year period. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Impact factor; Brazilian journals; Journal visibility.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900001

Highly sensitive C-reactive protein and male gender are independently related to the severity of coronary disease in patients with metabolic syndrome and an acute coronary event
Provedor de dados: 56
Autores: Monteiro,C.M.C.; Pinheiro,L.F.; Izar,M.C.; Barros,S.W.; Vasco,M.B.; Fischer,S.M.; Povoa,R.M.; Brandão,S.A.; Santos,A.O.; Oliveira,L.; Carvalho,A.C.; Fonseca,F.A.H.
Patients with metabolic syndrome are at high-risk for development of atherosclerosis and cardiovascular events. The objective of this study was to examine the major determinants of coronary disease severity, including those coronary risk factors associated with metabolic syndrome, during the early period after an acute coronary episode. We tested the hypothesis that inflammatory markers, especially highly sensitive C-reactive protein (hsCRP), are related to coronary atherosclerosis, in addition to traditional coronary risk factors. Subjects of both genders aged 30 to 75 years (N = 116) were prospectively included if they had suffered a recent acute coronary syndrome (acute myocardial infarction or unstable angina pectoris requiring hospitalization) and if...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic syndrome; Acute coronary syndrome; C-reactive protein; Gender; Gensini score.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300012

Facilitation of the main generator source of earthworm muscle contraction by a peripheral neuron
Provedor de dados: 56
Autores: Chang,Y.C.; Assmé,Z.; Bartoszeck,A.B.
A constant facilitation of responses evoked in the earthworm muscle contraction generator neurons by responses evoked in the neurons of its peripheral nervous system was demonstrated. It is based on the proposal that these two responses are bifurcations of an afferent response evoked by the same peripheral mechanical stimulus but converging again on this central neuron. A single-peaked generator response without facilitation was demonstrated by sectioning the afferent route of the peripheral facilitatory modulatory response, or conditioning response (CR). The multipeaked response could be restored by restimulating the sectioned modulatory neuron with an intracellular substitutive conditioning stimulus (SCS). These multi-peaked responses were proposed to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peri-Kästchen neuron; Facilitation of main generator response; Single and multipeaked responses.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000010

Identification of Zika virus in immature phases of Aedes aegypti and Aedes albopictus: a surveillance strategy for outbreak anticipation
Provedor de dados: 56
Autores: Maniero,V.C.; Rangel,P.S.C.; Coelho,L.M.C.; Silva,C.S.B.; Aguiar,R.S.; Lamas,C.C.; Cardozo,S.V.
A progressive increase in the circulation of arboviruses in tropical countries has been observed, accounting for 700,000 yearly deaths in the world. The main objective of this article was to identify the presence of Zika (ZIKV), dengue (DENV), and Chikungunya (CHIKV) viruses in immature stages of Aedes aegypti and Ae. albopictus. Household collections of immature phases of the vectors were carried out in the years 2015 and 2016. A total of 2902 dwellings were visited and the rate of infestation with larvae and pupae of Aedes mosquitoes was 283/1462 (19.4%) in March 2015 and 55/1440 (3.8%) in June 2015. In March 2015, 907 larvae/pupae were collected (583 or 64.3% of Ae. aegypti and 324 or 35.7% of Ae. albopictus) while in June 2015 there was a reduction in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zika virus; Aedes aegypti; Aedes albopictus; Arbovirus; Georeferencing; Xenomonitoring.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100605

Dialysis, time and death: comparisons of two consecutive decades among patients treated at the same Brazilian dialysis center
Provedor de dados: 56
Autores: De-Lima,J.J.G.; da-Fonseca,J.A.; Godoy,A.D.
The survival of hemodialysis patients is likely to be influenced not only by well-known risk factors like age and comorbidity, but also by changes in dialysis technology and practices accumulated along time. We compared the survival curves, dialysis routines and some risk factors of two groups of patients admitted to a Brazilian maintenance hemodialysis program during two consecutive decades: March 1977 to December 1986 (group 1, N = 162) and January 1987 to June 1997 (group 2, N = 237). The median treatment time was 22 months (range 1-198). Survival curves were constructed using the Kaplan-Meier method and compared using the log-rank method. The Cox proportional hazard regression model was used to investigate the more important variables associated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic renal failure; Hemodialysis; Kaplan-Meier survival curves; Risk factors.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300007

Errors in nonword repetition: bridging short- and long-term memory
Provedor de dados: 56
Autores: Santos,F.H.; Bueno,O.F.A.; Gathercole,S.E.
According to the working memory model, the phonological loop is the component of working memory specialized in processing and manipulating limited amounts of speech-based information. The Children's Test of Nonword Repetition (CNRep) is a suitable measure of phonological short-term memory for English-speaking children, which was validated by the Brazilian Children's Test of Pseudoword Repetition (BCPR) as a Portuguese-language version. The objectives of the present study were: i) to investigate developmental aspects of the phonological memory processing by error analysis in the nonword repetition task, and ii) to examine phoneme (substitution, omission and addition) and order (migration) errors made in the BCPR by 180 normal Brazilian children of both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Children; Working memory; Phonological memory; Nonword repetition; Language acquisition; Phonological loop.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300008

The expression of the ACTH receptor
Provedor de dados: 56
Autores: Elias,L.L.K.; Clark,A.J.L.
Adrenal glucocorticoid secretion is regulated by adrenocorticotropic hormone (ACTH) acting through a specific cell membrane receptor (ACTH-R). The ACTH-R is a member of the G protein superfamily-coupled receptors and belongs to the subfamily of melanocortin receptors. The ACTH-R is mainly expressed in the adrenocortical cells showing a restricted tissue specificity, although ACTH is recognized by the other four melanocortin receptors. The cloning of the ACTH-R was followed by the study of this gene in human diseases such as familial glucocorticoid deficiency (FGD) and adrenocortical tumors. FGD is a rare autosomal recessive disease characterized by glucocorticoid deficiency, elevated plasma ACTH levels and preserved renin/aldosterone secretion. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ACTH receptor; Resistance; Mutation; Transfection.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000015

Erratum for: “Participation of hepatic α/β-adrenoceptors and AT1 receptors in glucose release and portal hypertensive response induced by adrenaline or angiotensin II” [Braz J Med Biol Res (2018) 51(12): e7526]
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/other
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200901

Nonconventional amide bond formation catalysis: programming enzyme specificity with substrate mimetics
Provedor de dados: 56
Autores: Bordusa,F.
This article reports on the design and characteristics of substrate mimetics in protease-catalyzed reactions. Firstly, the basis of protease-catalyzed peptide synthesis and the general advantages of substrate mimetics over common acyl donor components are described. The binding behavior of these artificial substrates and the mechanism of catalysis are further discussed on the basis of hydrolysis, acyl transfer, protein-ligand docking, and molecular dynamics studies on the trypsin model. The general validity of the substrate mimetic concept is illustrated by the expansion of this strategy to trypsin-like, glutamic acid-specific, and hydrophobic amino acid-specific proteases. Finally, opportunities for the combination of the substrate mimetic strategy with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioorganic chemistry; Enzyme catalysis; Peptide synthesis; Proteases; Substrate mimetics.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500001

Eradication of Helicobacter pylori infection in patients with duodenal ulcer and non-ulcer dyspepsia and analysis of one-year reinfection rates
Provedor de dados: 56
Autores: Della Libera,E.; Rohr,M.R.S.; Moraes,M.; Siqueira,E.S.; Ferrari Jr.,A.P.
Helicobacter pylori (HP) infection is endemic worldwide. The proposed treatment is expensive and there are few reports regarding reinfection rates in Brazil. The aim of this study was to compare the eradication rates obtained with two therapeutic options and to evaluate reinfection one year after treatment. This was a prospective randomized trial with 55 patients. Thirty-nine patients had active duodenal ulcer (DU) and 16 non-ulcer dyspepsia (NUD), and all tested positive for HP. Diagnosis was based on at least two positive tests: ultrarapid urease test, histology and/or culture. Patients were randomized to two groups: group OMC treated with 40 mg omeprazole (once a day), 500 mg metronidazole and 250 mg clarithromycin (twice daily) for 7 days, or group NA...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Helicobacter pylori; Peptic disease; Non-ulcer dyspepsia; Gastritis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600008

Co-culture of apoptotic breast cancer cells with immature dendritic cells: a novel approach for DC-based vaccination in breast cancer
Provedor de dados: 56
Autores: Zheng,Jin; Liu,Qiang; Yang,Jiandong; Ren,Qinyou; Cao,Wei; Yang,Jingyue; Yu,Zhaocai; Yu,Fang; Wu,Yanlan; Shi,Hengjun; Liu,Wenchao.
A dendritic cell (DC)-based vaccine strategy could reduce the risk of recurrence and improve the survival of breast cancer patients. However, while therapy-induced apoptosis of hepatocellular and colorectal carcinoma cells can enhance maturation and antigen presentation of DCs, whether this effect occurs in breast cancer is currently unknown. In the present study, we investigated the effect of doxorubicin (ADM)-induced apoptotic MCF-7 breast cancer cells on the activation of DCs. ADM-induced apoptotic MCF-7 cells could effectively induce immature DC (iDC) maturation. The mean fluorescence intensity (MFI) of DC maturity marker CD83 was 23.3 in the ADM-induced apoptotic MCF-7 cell group compared with 8.5 in the MCF-7 cell group. The MFI of DC co-stimulatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Dendritic cells; Immunotherapy; Apoptosis; Doxorubicin.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600006

Main features of DNA-based immunization vectors
Provedor de dados: 56
Autores: Azevedo,V.; Levitus,G.; Miyoshi,A.; Cândido,A.L.; Goes,A.M.; Oliveira,S.C.
DNA-based immunization has initiated a new era of vaccine research. One of the main goals of gene vaccine development is the control of the levels of expression in vivo for efficient immunization. Modifying the vector to modulate expression or immunogenicity is of critical importance for the improvement of DNA vaccines. The most frequently used vectors for genetic immunization are plasmids. In this article, we review some of the main elements relevant to their design such as strong promoter/enhancer region, introns, genes encoding antigens of interest from the pathogen (how to choose and modify them), polyadenylation termination sequence, origin of replication for plasmid production in Escherichia coli, antibiotic resistance gene as selectable marker,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic immunization; DNA plasmid; Enhancer/promoter region; Immunostimulatory sequences (ISS); Antigens; Immune response.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200002

Analysis of polymorphism at site -174 G/C of interleukin-6 promoter region in multiple myeloma
Provedor de dados: 56
Autores: Duch,C.R.; Figueiredo,M.S.; Ribas,C.; Almeida,M.S.S.; Colleoni,G.W.B.; Bordin,J.O.
It is well established that interleukin-6 (IL-6) is an essential growth factor for multiple myeloma (MM) and patients with increased IL-6 levels have a poor prognosis. In healthy subjects, the presence of the C allele at a polymorphic site (-174 G/C) of the IL-6 gene is related to low IL-6 levels. In view of the potential association of this particular polymorphism with IL-6 concentration, and the relevance of IL-6 in MM pathogenesis, the objective of the present study was to investigate the prevalence of IL-6 (-174 G/C) promoter polymorphism and its association with development of MM in Brazilian individuals. We investigated the prevalence of these alleles in 52 patients and 60 healthy subjects (matched by age, sex, and race) of a Brazilian population....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Multiple myeloma; Interleukin-6; Polymorphisms.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200014

Association between apolipoprotein E genotype, serum lipids, and colorectal cancer in Brazilian individuals
Provedor de dados: 56
Autores: Souza,D.R.S.; Nakazone,M.A.; Pinhel,M.A.S.; Alvares,R.M.; Monaco,A.C.; Pinheiro,A.; Barros,C.F.D.C.; Cury,P.M.; Cunrath,G.S.; Netinho,J.G.
We evaluated genetic variants of apolipoprotein E (APOE HhaI) and their association with serum lipids in colorectal cancer (CRC), together with eating habits and personal history. Eight-seven adults with CRC and 73 controls were studied. APOE*2 (rs7412) and APOE*4 (rs429358) were identified by polymerase chain reaction-restriction fragment length polymorphism. APOE gene polymorphisms were similar in both groups, but the ε4/ε4 genotype (6%) was present only in controls. The patients had reduced levels (mean ± SD) of total cholesterol and low-density lipoprotein cholesterol fraction (180.4 ± 49.5 and 116.1 ± 43.1 mg/dL, respectively) compared to controls (204.2 ± 55.6, P = 0.135 and 134.7 ± 50.8 mg/dL; P = 0.330, respectively) indicating that they were not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein E; Colorectal cancer; Lipid profile; Polymorphisms.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500001

Thermodynamic evaluation and modeling of proton and water exchange associated with benzamidine and berenil binding to ß-trypsin
Provedor de dados: 56
Autores: Pereira,M.T.; Silva-Alves,J.M.; Martins-José,A.; Lopes,J.C.D.; Santoro,M.M.
Serine-proteases are involved in vital processes in virtually all species. They are important targets for researchers studying the relationships between protein structure and activity, for the rational design of new pharmaceuticals. Trypsin was used as a model to assess a possible differential contribution of hydration water to the binding of two synthetic inhibitors. Thermodynamic parameters for the association of bovine ß-trypsin (homogeneous material, observed 23,294.4 ± 0.2 Da, theoretical 23,292.5 Da) with the inhibitors benzamidine and berenil at pH 8.0, 25ºC and with 25 mM CaCl2, were determined using isothermal titration calorimetry and the osmotic stress method. The association constant for berenil was about 12 times higher compared to the one for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Benzamidine; Berenil; Calorimetry; Protein modeling; Trypsin; Osmotic stress.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100005

Application of isotope-selective non-dispersive infrared spectrometry for the evaluation of the 13C-urea breath test: comparison with three concordant methods
Provedor de dados: 56
Autores: Coelho,L.G.V.; Reber,M.; Passos,M.C.F.; Aguiar,R.O.A.; Casaes,P.E.; Bueno,M.L.; Yazaki,F.R.; Castro,F.J.; Vieira,W.L.S.; Franco,J.M.M.; Castro,L.P.
The aim of this work was to compare the performance of isotope-selective non-dispersive infrared spectrometry (IRIS) for the 13C-urea breath test with the combination of the 14C-urea breath test (14C-UBT), urease test and histologic examination for the diagnosis of H. pylori (HP) infection. Fifty-three duodenal ulcer patients were studied. All patients were submitted to gastroscopy to detect HP by the urease test, histologic examination and 14C-UBT. To be included in the study the results of the 3 tests had to be concordant. Within one month after admission to the study the patients were submitted to IRIS with breath samples collected before and 30 min after the ingestion of 75 mg 13C-urea dissolved in 200 ml of orange juice. The samples were mailed and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 13C-urea breath test; Infrared spectrometry.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200007

Detection and analysis of apoptosis in peripheral blood cells from breast cancer patients
Provedor de dados: 56
Autores: Simão,T.A.; Andrada-Serpa,M.J.; Mendonça,G.A.S.; Marques,D.D.; Braga,M.A.; Santos,A.L.S.; Amorim,L.M.F.; Cardoso,L.E.B.; Carmo,P.A.O.; De-Moura-Gallo,C.V.
Apoptosis is a well-known specific process of cell death that normally occurs in physiological situations such as tissue or organ development and involution. During tumor growth there is a balance between proliferation and cell death which involves apoptotic mechanisms. In the present study genomic DNAs from 120 breast tumor biopsies were analyzed by agarose gel electrophoresis and none of them presented the fragmentation pattern characteristic of the apoptosis process. However, 33% of the 105 breast cancer patients clearly showed the apoptotic pattern when DNA from blood cells was analyzed. None of the DNAs from healthy volunteer blood cells showed any trace of apoptosis. Since the breast cancer patients were not receiving chemo- or hormone therapy, the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Apoptosis; Breast cancer; Peripheral blood cells; Cortisol; Fas-system.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400004

Malaria vaccine: roadblocks and possible solutions
Provedor de dados: 56
Autores: Soares,I.S.; Rodrigues,M.M.
Malaria remains the most prevalent and devastating parasitic disease worldwide. Vaccination is considered to be an approach that will complement other strategies for prevention and control of the disease in the future. In the last 10 years, intense studies aimed at the development of a malaria vaccine have provided important knowledge of the nature of the host immunological mechanisms of protection and their respective target antigens. It became well established that protective immune responses can be generated against the distinct stages of Plasmodium. However, in general, protective immune responses are directed at stage-specific antigens. The elucidation of the primary structure of these antigens made possible the generation of synthetic and recombinant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malaria; Plasmodium; Vaccine; Immunity; Infectious disease.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300002

Vascular supply of the central nervous system of the land snail Megalobulimus oblongus (Gastropoda, Pulmonata)
Provedor de dados: 56
Autores: Nóblega,H.G.; Missaglia,V.; Stenert,C.; Faccioni-Heuser,M.C.; Achaval,M.
The vascularization of the central nervous system of the snail Megalobulimus oblongus was studied by injection of carmine-gelatin solution into the arterial system and using a histochemical technique for the detection of alkaline phosphatase. The central nervous system of M. oblongus is irrigated by the anterior aorta, from which a series of small branches emerge that supply the subesophageal nervous ganglia. In turn, these branches give rise to a series of smaller vessels that irrigate the buccal bulb, the anterior portion of the foot, the cerebral ganglia, the dorsal body gland, and the anterior portion of the reproductive system. No hemolymph vessels were detected within nervous tissue although such vessels were found in the periganglionic connective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastropods; Central nervous system; Vascular supply; Trypan blue; Carmine-gelatin; Alkaline phosphatase activity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900016

Cytokine profile and pathology in human leishmaniasis
Provedor de dados: 56
Autores: Ribeiro-de-Jesus,A.; Almeida,R.P.; Lessa,H.; Bacellar,O.; Carvalho,E.M.
The clinical spectrum of leishmaniasis and control of the infection are influenced by the parasite-host relationship. The role of cellular immune responses of the Th1 type in the protection against disease in experimental and human leishmaniasis is well established. In humans, production of IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; is associated with the control of infection in children infected by Leishmania chagasi. In visceral leishmaniasis, an impairment in IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; production and high IL-4 and IL-10 levels (Th2 cytokines) are observed in antigen-stimulated peripheral blood mononuclear cells (PBMC). Moreover, IL-12 restores IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; production and enhances the cytotoxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Human leishmaniasis; Cytokines; Pathology; Immunological responses.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100020

Effects of natural aging and gender on pro-inflammatory markers
Provedor de dados: 56
Autores: Milan-Mattos,J.C.; Anibal,F.F.; Perseguini,N.M.; Minatel,V.; Rehder-Santos,P.; Castro,C.A.; Vasilceac,F.A.; Mattiello,S.M.; Faccioli,L.H.; Catai,A.M.
The term inflammaging is now widely used to designate the inflammatory process of natural aging. During this process, cytokine balance is altered, presumably due to the loss of homeostasis, thus contributing to a greater predisposition to disease and exacerbation of chronic diseases. The aim of the study was to analyze the relationship between pro-inflammatory markers and age in the natural aging process of healthy individuals. One hundred and ten subjects were divided into 5 groups according to age (22 subjects/group). Interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) were quantified using the ELISA method. High-sensitivity C-reactive protein (hsCRP) was analyzed by turbidimetry according to laboratory procedures. The main findings of this study...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Natural aging; Inflammaging; Immune system; HsCRP; IL-6.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900601

Plasma and testicular testosterone levels, volume density and number of Leydig cells and spermatogenic efficiency of rabbits
Provedor de dados: 56
Autores: Castro,A.C.S.; Berndtson,W.E.; Cardoso,F.M.
Plasma and tissue testosterone concentrations were determined by radioimmunoassay in 12 eight-month-old sexually mature New Zealand White rabbits and evaluated for possible associations with spermatogenic efficiency as well as with volume density and number of Leydig cells. Testicular tissue was processed histologically and histometry was performed in order to quantify germ cells, Sertoli cells and Leydig cells. Spermatogenic efficiency, reported as the ratios among germ cells (spermatogonia, primary spermatocytes and round spermatids) and by the ratio of germ cells to Sertoli cells, was not associated with testosterone levels. However, Leydig cell parameters such as number of Leydig cells per gram of testis, total number of Leydig cells per testis and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabbit; Testosterone; Leydig cell; Spermatogenesis; Histometry.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400014

Anticonvulsant and proconvulsant roles of nitric oxide in experimental epilepsy models
Provedor de dados: 56
Autores: Del-Bel,E.A.; Oliveira,P.R.; Oliveira,J.A.C.; Mishra,P.K.; Jobe,P.C.; Garcia-Cairasco,N.
The effect of acute (120 mg/kg) and chronic (25 mg/kg, twice a day, for 4 days) intraperitonial injection of the nitric oxide (NO) synthase (NOS) inhibitor NG-nitro-L-arginine (L-NOARG) was evaluated on seizure induction by drugs such as pilocarpine and pentylenetetrazole (PTZ) and by sound stimulation of audiogenic seizure-resistant (R) and audiogenic seizure-susceptible (S) rats. Seizures were elicited by a subconvulsant dose of pilocarpine (100 mg/kg) only after NOS inhibition. NOS inhibition also simultaneously potentiated the severity of PTZ-induced limbic seizures (60 mg/kg) and protected against PTZ-induced tonic seizures (80 mg/kg). The audiogenic seizure susceptibility of S or R rats did not change after similar treatments. In conclusion,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental epilepsy; Seizures; Forebrain; Brainstem; Pilocarpine; Pentylenetetrazol; Audiogenic seizures; Genetically epilepsy-prone rats; GEPR; Nitric oxide.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800010

The role of CD8+ T cells during allograft rejection
Provedor de dados: 56
Autores: Bueno,V.; Pestana,J.O.M.
Organ transplantation can be considered as replacement therapy for patients with end-stage organ failure. The percent of one-year allograft survival has increased due, among other factors, to a better understanding of the rejection process and new immunosuppressive drugs. Immunosuppressive therapy used in transplantation prevents activation and proliferation of alloreactive T lymphocytes, although not fully preventing chronic rejection. Recognition by recipient T cells of alloantigens expressed by donor tissues initiates immune destruction of allogeneic transplants. However, there is controversy concerning the relative contribution of CD4+ and CD8+ T cells to allograft rejection. Some animal models indicate that there is an absolute requirement for CD4+ T...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transplantation; Rejection; T cells; Cytokines; Chemokines.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100001

13CO2 recovery fraction in expired air of septic patients under mechanical ventilation
Provedor de dados: 56
Autores: Martins,M.A.; Coletto,F.A.; Martins-Filho,O.A.; Marchini,J.S.; Basile-Filho,A.
The continuous intravenous administration of isotopic bicarbonate (NaH13CO2) has been used for the determination of the retention of the 13CO2 fraction or the 13CO2 recovered in expired air. This determination is important for the calculation of substrate oxidation. The aim of the present study was to evaluate, in critically ill patients with sepsis under mechanical ventilation, the 13CO2 recovery fraction in expired air after continuous intravenous infusion of NaH13CO2 (3.8 µmol/kg diluted in 0.9% saline in ddH2O). A prospective study was conducted on 10 patients with septic shock between the second and fifth day of sepsis evolution (APACHE II, 25.9 ± 7.4). Initially, baseline CO2 was collected and indirect calorimetry was also performed. A primer of 5 mL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recovery fraction; 13CO2; Stable isotope; Septic shock; Intensive care setting.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700003

Expression of neuronal nitric oxide synthase in the developing superficial layers of the rat superior colliculus
Provedor de dados: 56
Autores: Giraldi-Guimarães,A.; Bittencourt-Navarrete,R.E.; Mendez-Otero,R.
We investigated the level of expression of neuronal nitric oxide synthase (nNOS) in the retinorecipient layers of the rat superior colliculus during early postnatal development. Male and female Lister rats ranging in age between the day of birth (P0) and the fourth postnatal week were used in the present study. Two biochemical methods were used, i.e., in vitro measurement of NOS specific activity by the conversion of [³H]-arginine to [³H]-citrulline, and analysis of Western blotting immunoreactive bands from superior colliculus homogenates. As revealed by Western blotting, very weak immunoreactive bands were observed as early as P0-2, and their intensity increased progressively at least until P21. The analysis of specific activity of NOS showed similar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superior colliculus; Nitric oxide synthase; Development; Retinotectal projection.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600013

Manipulation of a quasi-natural cell block for high-efficiency transplantation of adherent somatic cells
Provedor de dados: 56
Autores: Chung,H.J.; Hassan,M.M.; Park,J.O.; Kim,H.J.; Hong,S.T.
Recent advances have raised hope that transplantation of adherent somatic cells could provide dramatic new therapies for various diseases. However, current methods for transplanting adherent somatic cells are not efficient enough for therapeutic applications. Here, we report the development of a novel method to generate quasi-natural cell blocks for high-efficiency transplantation of adherent somatic cells. The blocks were created by providing a unique environment in which cultured cells generated their own extracellular matrix. Initially, stromal cells isolated from mice were expanded in vitro in liquid cell culture medium followed by transferring the cells into a hydrogel shell. After incubation for 1 day with mechanical agitation, the encapsulated cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quasi-natural cell block; Transplantation; Scaffold; Extracellular matrix; Hydrogel.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500392

Differential role of entorhinal and hippocampal nerve growth factor in short- and long-term memory modulation
Provedor de dados: 56
Autores: Walz,R.; Roesler,R.; Reinke,A.; Martins,M.R.; Quevedo,J.; Izquierdo,I.
We studied the effects of infusion of nerve growth factor (NGF) into the hippocampus and entorhinal cortex of male Wistar rats (250-300 g, N = 11-13 per group) on inhibitory avoidance retention. In order to evaluate the modulation of entorhinal and hippocampal NGF in short- and long-term memory, animals were implanted with cannulae in the CA1 area of the dorsal hippocampus or entorhinal cortex and trained in one-trial step-down inhibitory avoidance (foot shock, 0.4 mA). Retention tests were carried out 1.5 h or 24 h after training to measure short- and long-term memory, respectively. Immediately after training, rats received 5 µl NGF (0.05, 0.5 or 5.0 ng) or saline per side into the CA1 area and entorhinal cortex. The correct position of the cannulae was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nerve growth factor; Hippocampus; Entorhinal cortex; Short- and long-term memory; Inhibitory avoidance; Rat.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100009

DNA damage by ochratoxin A in rat kidney assessed by the alkaline comet assay
Provedor de dados: 56
Autores: Zeljezic,D.; Domijan,A.-M.; Peraica,M.
There are few studies of ochratoxin A (OTA) genotoxicity in experimental animals and the results obtained with cell cultures are inconsistent, although the carcinogenic potential of OTA for the kidney of experimental animals has been well established. We studied the genotoxic potential of OTA in the kidney of adult female Wistar rats (5 in each group) treated intraperitoneally with OTA (0.5 mg kg body weight-1 day-1 for 7, 14, and 21 days) measuring DNA mobility on agarose gel stained with ethidium-bromide using standard alkaline single-cell gel electrophoresis (comet assay). Negative control animals were treated with solvent (Tris buffer, 1.0 mg/kg) and positive control animals were treated with methyl methanesulfonate (40 mg/kg) according to the same...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; DNA adducts; Experimental animals; Mycotoxin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200006

Effects of submaximal exercise with water ingestion on intraocular pressure in healthy human males
Provedor de dados: 56
Autores: Moura,M.A.; Rodrigues,L.O.C.; Waisberg,Y.; de Almeida,H.G.; Silami-Garcia,E.
The effects of exercise and water replacement on intraocular pressure (IOP) have not been well established. Furthermore, it is not known whether the temperature of the fluid ingested influences the IOP response. In the present study we determined the effect of water ingestion at three temperatures (10, 24 and 38ºC; 600 ml 15 min before and 240 ml 15, 30 and 45 min after the beginning of each experimental session) on the IOP of six healthy male volunteers (age = 24.0 ± 3.5 years, weight = 67.0 ± 4.8 kg, peak oxygen uptake (VO2peak) = 47.8 ± 9.1 ml kg-1 min-1). The subjects exercised until exhaustion on a cycle ergometer at a 60% VO2peak in a thermoneutral environment. IOP was measured before and after exercise and during recovery (15, 30 and 45 min) using...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Exercise; Intraocular pressure; Thermal regulation; Water ingestion.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100017

Functions of the extracellular matrix and matrix degrading proteases during tumor progression
Provedor de dados: 56
Autores: Liaw,L.; Crawford,H.C.
Cell interactions with extracellular matrices are important to pathological changes that occur during cell transformation and tumorigenesis. Several extracellular matrix proteins including fibronectin, thrombospondin-1, laminin, SPARC, and osteopontin have been suggested to modulate tumor phenotype by affecting cell migration, survival, or angiogenesis. Likewise, proteases including the matrix metalloproteinases (MMPs) are understood to not only facilitate migration of cells by degradation of matrices, but also to affect tumor formation and growth. We have recently demonstrated an in vivo role for the RGD-containing protein, osteopontin, during tumor progression, and found evidence for distinct functions in the host versus the tumor cells. Because of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Matrix metalloproteinase; Osteopontin; Carcinoma; Protease.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700002

Critical steps in the early evolution of the isocortex: Insights from developmental biology
Provedor de dados: 56
Autores: Aboitiz,F.; Montiel,J.; López,J.
This article proposes a comprehensive view of the origin of the mammalian brain. We discuss i) from which region in the brain of a reptilian-like ancestor did the isocortex originate, and ii) the origin of the multilayered structure of the isocortex from a simple-layered structure like that observed in the cortex of present-day reptiles. Regarding question i there have been two alternative hypotheses, one suggesting that most or all the isocortex originated from the dorsal pallium, and the other suggesting that part of the isocortex originated from a ventral pallial component. The latter implies that a massive tangential migration of cells from the ventral pallium to the dorsal pallium takes place in isocortical development, something that has not been...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cdk5; Dorsal ventricular ridge; Reelin; Tangential migration; Ventral pallium.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001200006

Aversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male rats
Provedor de dados: 56
Autores: Aguiar,C.E.; Cadore,L.P.; Padoin,M.J.; Barbosa-Coutinho,L.M.; Lucion,A.B.
Immunohistochemistry was used to evaluate the effects of neonatal handling and aversive stimulation during the first 10 days of life on the number of corticotrophs in the anterior lobe of the pituitary of 11-day-old male Wistar rats. Since adult rats handled during infancy respond with reduced corticosterone secretion in response to stressors and with less behavior inhibition in novel environments, we assumed that neonatal stimulation could affect pituitary morphology during this critical period of cell differentiation. Three groups of animals were studied: intact (no manipulation, N = 5), handled (N = 5) and stimulated (submitted to 3 different aversive stimuli, N = 5). The percentage of ACTH-immunoreactive cells in the anterior lobe of the pituitary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ACTH; Immunohistochemistry; Pituitary; Neonatal handling; Stress.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200013

Coexistence of potentiation and fatigue in skeletal muscle
Provedor de dados: 56
Autores: Rassier,D.E.; MacIntosh,B.R.
Twitch potentiation and fatigue in skeletal muscle are two conditions in which force production is affected by the stimulation history. Twitch potentiation is the increase in the twitch active force observed after a tetanic contraction or during and following low-frequency stimulation. There is evidence that the mechanism responsible for potentiation is phosphorylation of the regulatory light chains of myosin, a Ca2+-dependent process. Fatigue is the force decrease observed after a period of repeated muscle stimulation. Fatigue has also been associated with a Ca2+-related mechanism: decreased peak Ca2+ concentration in the myoplasm is observed during fatigue. This decrease is probably due to an inhibition of Ca2+ release from the sarcoplasmic reticulum....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Posttetanic potentiation; Staircase; High-frequency fatigue; Low-frequency fatigue; Ca2+ sensitivity; RLC phosphorylation; Myosin light chains.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500003

Effect of lung resection and sham surgery on the frequency of infection in alloxan-diabetic rats
Provedor de dados: 56
Autores: Seidel,A.C.; Fagundes,D.J.; Bazotte,R.B.; Novo,N.F.; Juliano,Y.; Meister,H.
The present study was carried out in order to determine the effect of lung resection on the frequency of infections in alloxan-diabetic rats. Adult female Wistar rats were injected with alloxan (40 mg/kg, iv) to induce diabetes mellitus (group D; N = 45) or with vehicle (1.0 ml/kg, iv) to be used as controls (group C; N = 45). Thirty-six days after receiving alloxan both groups were randomly divided into three subgroups: no operation (NO; N = 15), sham operation (SO; N = 15), and left pneumonectomy (PE; N = 15). The rats were sacrificed 36 days after surgery and their lungs were examined microscopically and macroscopically. The occurrence of thoracic wall infection, thoracic wall abscess, lung abscess and pleural empyema was similar in groups D and C. In...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diabetes; Alloxan; Pneumonectomy; Rats; Infection.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300001

The fallacy of racial pharmacogenomics
Provedor de dados: 56
Autores: Pena,S.D.J.
Personalized pharmacogenomics aims to use individual genotypes to direct medical treatment. Unfortunately, the loci relevant for the pharmacokinetics and especially the pharmacodynamics of most drugs are still unknown. Moreover, we still do not understand the role that individual genotypes play in modulating the pathogenesis, the clinical course and the susceptibility to drugs of human diseases which, although appearing homogeneous on the surface, may vary from patient to patient. To try to deal with this situation, it has been proposed to use interpopulational variability as a reference for drug development and prescription, leading to the development of "race-targeted drugs". Given the present limitations of genomic knowledge and of the tools needed to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: DNA; Genetic polymorphisms; Human genomic variability; Race; Pharmacogenomics.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400001

Impairment of locomotor activity induced by the novelN-acylhydrazone derivatives LASSBio-785 and LASSBio-786 in mice
Provedor de dados: 56
Autores: Silva,G.A.P.; Kummerle,A.E.; Antunes,F.; Fraga,C.A.M.; Barreiro,E.J.; Zapata-Sudo,G.; Sudo,R.T.
The N-acylhydrazone (NAH) analogues N-methyl 2-thienylidene 3,4-benzoylhydrazine (LASSBio-785) and N-benzyl 2-thienylidene 3,4-benzoylhydrazine (LASSBio-786) were prepared from 2-thienylidene 3,4-methylenedioxybenzoylhydrazine (LASSBio-294). The ability of LASSBio-785 and LASSBio-786 to decrease central nervous system activity was investigated in male Swiss mice. LASSBio-785 or LASSBio-786 (30 mg/kg, ip) reduced locomotor activity from 209 ± 26 (control) to 140 ± 18 (P &lt; 0.05) or 146 ± 15 crossings/min (P &lt; 0.05), respectively. LASSBio-785 (15 or 30 mg/kg, iv) also reduced locomotor activity from 200 ± 15 to 116 ± 29 (P &lt; 0.05) or 60 ± 16 crossings/min (P &lt; 0.01), respectively. Likewise, LASSBio-786 (15 or 30 mg/kg, iv) reduced locomotor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: N-acylhydrazone; Locomotor activity; Sedation; Hypnosis; Benzodiazepine receptor; Flumazenil.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300263

Nonspecific blockade of vascular free radical signals by methylated arginine analogues
Provedor de dados: 56
Autores: Pedro,M.A.; Augusto,O.; Barbeiro,H.V.; Carvalho,M.H.C.; da-Luz,P.L.; Laurindo,F.R.M.
Methylated arginine analogues are often used as probes of the effect of nitric oxide; however, their specificity is unclear and seems to be frequently overestimated. This study analyzed the effects of NG-methyl-L-arginine (L-NMMA) on the endothelium-dependent release of vascular superoxide radicals triggered by increased flow. Plasma ascorbyl radical signals measured by direct electron paramagnetic resonance spectroscopy in 25 rabbits increased by 3.8 ± 0.7 nmol/l vs baseline (28.7 ± 1.4 nmol/l, P&amp;lt;0.001) in response to papaverine-induced flow increases of 121 ± 12%. In contrast, after similar papaverine-induced flow increases simultaneously with L-NMMA infusions, ascorbyl levels were not significantly changed compared to baseline. Similar results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Free radicals; Arginine analogues; NG-methyl-L-arginine; NG-methyl-D-arginine; NG-nitro-L-arginine methyl ester.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600004

Methylmercury intoxication activates nitric oxide synthase in chick retinal cell culture
Provedor de dados: 56
Autores: Herculano,A.M.; Crespo-López,M.E.; Lima,S.M.A.; Picanço-Diniz,D.L.W.; Nascimento,J.L.M. Do.
The visual system is a potential target for methylmercury (MeHg) intoxication. Nevertheless, there are few studies about the cellular mechanisms of toxicity induced by MeHg in retinal cells. Various reports have indicated a critical role for nitric oxide synthase (NOS) activation in modulating MeHg neurotoxicity in cerebellar and cortical regions. The aim of the present study is to describe the effects of MeHg on cell viability and NOS activation in chick retinal cell cultures. For this purpose, primary cultures were prepared from 7-day-old chick embryos: retinas were aseptically dissected and dissociated and cells were grown at 37ºC for 7-8 days. Cultures were exposed to MeHg (10 µM, 100 µM, and 1 mM) for 2, 4, and 6 h. Cell viability was measured by MTT...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methylmercury; Chick retinal cell culture; Nitric oxide synthase; Cell viability; Neurotoxicity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300013

Measurement of epidermal thickness in a patient with psoriasis by computer-supported image analysis
Provedor de dados: 56
Autores: Alper,M.; Kavak,A.; Parlak,A.H.; Demirci,R.; Belenli,I.; Yesildal,N.
The aim of the present study was to measure full epidermal thickness, stratum corneum thickness, rete length, dermal papilla widening and suprapapillary epidermal thickness in psoriasis patients using a light microscope and computer-supported image analysis. The data obtained were analyzed in terms of patient age, type of psoriasis, total body surface area involvement, scalp and nail involvement, duration of psoriasis, and family history of the disease. The study was conducted on 64 patients and 57 controls whose skin biopsies were examined by light microscopy. The acquired microscopic images were transferred to a computer and measurements were made using image analysis. The skin biopsies, taken from different body areas, were examined for different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psoriasis; Epidermis; Thickness; Computer-supported image analysis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100015

Effects of a neuronal nitric oxide synthase inhibitor on lipopolysaccharide-induced fever
Provedor de dados: 56
Autores: Perotti,C.A.A.; Nogueira,M.S.; Antunes-Rodrigues,J.; Cárnio,E.C.
It has been demonstrated that nitric oxide (NO) has a thermoregulatory action, but very little is known about the mechanisms involved. In the present study we determined the effect of neuronal nitric oxide synthase (nNOS) inhibition on thermoregulation. We used 7-nitroindazole (7-NI, 1, 10 and 30 mg/kg body weight), a selective nNOS inhibitor, injected intraperitoneally into normothermic Wistar rats (200-250 g) and rats with fever induced by lipopolysaccharide (LPS) (100 µg/kg body weight) administration. It has been demonstrated that the effects of 30 mg/kg of 7-NI given intraperitoneally may inhibit 60% of nNOS activity in rats. In all experiments the colonic temperature of awake unrestrained rats was measured over a period of 5 h at 15-min intervals...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; 7-nitroindazole; Body temperature; LPS; L-NAME.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100008

Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos
Provedor de dados: 56
Autores: Avram,Ş.F.; Cimpean,A.M.; Raica,M.
The P1.HTR cell line includes highly transfectable cells derived from P815 mastocytoma cells originating from mouse breast tissue. Despite its widespread use in immunogenic studies, no data are available about the behavior of P1.HTR cells in the chick embryo chorioallantoic membrane model. The objective of the present investigation was to study the effects of P1.HTR cells implanted on the chorioallantoic membrane of chick embryos. We inoculated P1.HTR cells into the previously prepared chick embryo chorioallantoic membrane and observed the early and late effects of these cells by stereomicroscopy, histochemistry and immunohistochemistry. A highly angiotropic and angiogenic effect occurred early after inoculation and a tumorigenic potential with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P1.HTR; Mastocytoma; Chick embryo chorioallantoic membrane; Angiogenesis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100052

Distribution of small proteoglycans and glycosaminoglycans in humerus-related articular cartilage of chickens
Provedor de dados: 56
Autores: Rodrigues,E.D.; Pimentel,E.R.; Mourão,P.A.S.; Gomes,L.
The expression of components present in the cartilaginous extracellular matrix is related to development, gender, and genotype, as well as to the biomechanical properties of each type of cartilage. In the present study, we analyzed small proteoglycans and glycosaminoglycans present in different cartilages of the chicken wing after extraction with guanidine hydrochloride or papain. Quantitative analysis of glycosaminoglycans showed a larger amount in humeral cartilage (around 200 mg/g tissue) than in articular cartilage of the radius and ulna, with 138 and 80 mg/g tissue, respectively. Non-collagenous proteins isolated were predominantly from cartilage in the proximal regions of the humerus and radius. D4 fractions obtained by ultracentrifugation were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proteoglycan; Fibromodulin; Decorin; Glycosaminoglycan; Cartilage; Chicken.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300009

Stem cell factor protects against neuronal apoptosis by activating AKT/ERK in diabetic mice
Provedor de dados: 56
Autores: Li,J.-W.; Li,L.-L.; Chang,L.-L.; Wang,Z.-Y.; Xu,Y.
Neuronal apoptosis occurs in the diabetic brain due to insulin deficiency or insulin resistance, both of which reduce the expression of stem cell factor (SCF). We investigated the possible involvement of the activation of the MAPK/ERK and/or AKT pathways in neuroprotection by SCF in diabetes. Male C57/B6 mice (20-25 g) were randomly divided into four groups of 10 animals each. The morphology of the diabetic brain in mice treated or not with insulin or SCF was evaluated by H&amp;E staining and TUNEL. SCF, ERK1/2 and AKT were measured by Western blotting. In diabetic mice treated with insulin or SCF, there was fewer structural change and apoptosis in the cortex compared to untreated mice. The apoptosis rate of the normal group, the diabetic group receiving...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stem cell factor; Diabetes; MAPK/ERK; AKT.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100008

Infusions of AP5 into the basolateral amygdala impair the formation, but not the expression, of step-down inhibitory avoidance
Provedor de dados: 56
Autores: Roesler,R.; Vianna,M.R.M.; de-Paris,F.; Quevedo,J.; Walz,R.; Bianchin,M.
We evaluated the effects of infusions of the NMDA receptor antagonist D,L-2-amino-5-phosphonopentanoic acid (AP5) into the basolateral nucleus of the amygdala (BLA) on the formation and expression of memory for inhibitory avoidance. Adult male Wistar rats (215-300 g) were implanted under thionembutal anesthesia (30 mg/kg, ip) with 9.0-mm guide cannulae aimed 1.0 mm above the BLA. Bilateral infusions of AP5 (5.0 µg) were given 10 min prior to training, immediately after training, or 10 min prior to testing in a step-down inhibitory avoidance task (0.3 mA footshock, 24-h interval between training and the retention test session). Both pre- and post-training infusions of AP5 blocked retention test performance. When given prior to the test, AP5 did not affect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amygdala; NMDA receptor; Fear; Memory.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700014

Functional dyspepsia: relationship between clinical subgroups and Helicobacter pylori status in Western Turkey
Provedor de dados: 56
Autores: Saruc,M.; Ozden,N.; Turkel,N.; Ayhan,S.; Demir,M.A.; Tuzcuoglu,I.; Akarca,U.S.; Yuceyar,H.
The etiology of functional dyspepsia is not known. The objective of the present study was to determine the characteristics of functional dyspepsia in Western Turkey. We divided 900 patients with functional dyspepsia into three subgroups according to symptoms: ulcer-like (UL), 321 (35.6%), motility disorder-like (ML), 281 (31.2%), and the combination (C) of these symptoms, 298 (33.1%). All patients were submitted to endoscopic evaluation, with two biopsies taken from the cardia and corpus, and four from the antrum of the stomach. All biopsy samples were studied for Helicobacter pylori (Hp) density, chronic inflammation, activity, intestinal metaplasia, atrophy, and the presence of lymphoid aggregates by histological examination. One antral biopsy was used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional dyspepsia; Subgroups; Helicobacter pylori; Symptoms.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600010

Small RNA sequencing reveals microRNAs related to neuropathic pain in rats
Provedor de dados: 56
Autores: Dai,Dawei; Wang,Junyu; Jiang,Ying; Yuan,Lei; Lu,Youming; Zhang,Aijun; Zou,Dongdong; Chen,Xin.
The present study aimed to identify microRNAs (miRNAs) that are involved in neuropathic pain and predict their corresponding roles in the pathogenesis and development process of neuropathic pain. The rat model of neuropathic pain caused by spared nerve injury (SNI) was established in Sprague-Dawley male rats, followed by small RNA sequencing of the L3–L6 dorsal root ganglion. Real-time PCR was performed to validate the differently expressed miRNAs. Functional verification was performed by intrathecally injecting the animals with miRNA agomir. A total of 72 differentially expressed miRNAs were identified in the SNI rats, including 33 upregulated and 39 downregulated miRNAs. The results of qPCR further verified the expression levels of rno-miR-6215...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNAs; Neuropathic pain; Spared nerve injury; Dorsal root ganglion.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000602

Effects of Lippia sidoides essential oil, thymol, p-cymene, myrcene and caryophyllene on rat sciatic nerve excitability
Provedor de dados: 56
Autores: Barbosa,R.; Cruz-Mendes,Y.; Silva-Alves,K.S.; Ferreira-da-Silva,F.W.; Ribeiro,N.M.; Morais,L.P.; Leal-Cardoso,J.H.
Lippia sidoides Cham is a typical herb species of Northeast Brazil with widespread use in folk medicine. The major constituents of the essential oil of L. sidoides (EOLs) are thymol, p-cymene, myrcene, and caryophyllene. Several studies have shown that the EOLs and its constituents have pharmacological effects, including antibacterial, anti-inflammatory, antioxidant and neuroprotective activity. Therefore, this work aimed to investigate the effects of the EOLs and their main constituents on rat sciatic nerve excitability. The sciatic nerves of adult Wistar rats were dissected and mounted in a moist chamber. Nerves were stimulated by square wave pulses, with an amplitude of 40 V, duration of 100 μs to 0.2 Hz. Both EOLs and thymol inhibited compound action...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Compound action potential; Essential oil; Lippia sidoides; Thyme oil; P-cymene; Myrcene.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200611

Treatment with ascorbic acid and α-tocopherol modulates oxidative-stress markers in the spinal cord of rats with neuropathic pain
Provedor de dados: 56
Autores: Riffel,A.P.K.; Santos,M.C.Q.; de Souza,J.A.; Scheid,T.; Horst,A.; Kolberg,C.; Belló-Klein,A.; Partata,W.A.
Vitamin E (vit. E) and vitamin C (vit. C) are antioxidants that inhibit nociception. The effect of these vitamins on oxidative-stress markers in the spinal cord of rats with chronic constriction injury (CCI) of the sciatic nerve is unknown. This study investigated the effect of intraperitoneal administration of vit. E (15 mg·kg-1·day-1) and vit. C (30 mg·kg-1·day-1), given alone or in combination, on spinal cord oxidative-stress markers in CCI rats. Adult male Wistar rats weighing 200–250 g were divided equally into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Total thiol; Superoxide anion generation; Nitric oxide; Lipid hydroperoxides; Hydrogen peroxide; Total antioxidant capacity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400612

Reduced folate carrier 1 (RFC1) is associated with cleft of the lip only
Provedor de dados: 56
Autores: Vieira,A.R.; Cooper,M.E.; Marazita,M.L.; Castilla,E.E.; Orioli,I.M.
In this report, we have reanalyzed genotyping data in a collection of families from South America based on maternal origin. Genotyping analysis was performed at the Craniofacial Anomalies Research Center at the University of Iowa. These genotypes were derived from genomic DNA samples obtained from blood spots from children born with isolated orofacial clefts in 45 hospitals located in eight countries (Argentina, Bolivia, Brazil, Chile, Ecuador, Paraguay, Uruguay, and Venezuela) collaborating with ECLAMC (Latin American Collaborative Studies of Congenital Malformations) between January 1998 and December 1999. Dried blood samples were sent by regular mail to the Laboratory of Congenital Malformations, Federal University of Rio de Janeiro. Previous findings...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cleft lip and palate Reduced folate carrier 1 5; 10-Methylenetetrahydrofolate reductase Folate Oral clefts Mitochondrial DNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800009

Anethole reduces oxidative stress and improves in vitro survival and activation of primordial follicles
Provedor de dados: 56
Autores: Sá,N.A.R.; Bruno,J.B.; Guerreiro,D.D.; Cadenas,J.; Alves,B.G.; Cibin,F.W.S.; Leal-Cardoso,J.H.; Gastal,E.L.; Figueiredo,J.R.
Primordial follicles, the main source of oocytes in the ovary, are essential for the maintenance of fertility throughout the reproductive lifespan. To the best of our knowledge, there are no reports describing the effect of anethole on this important ovarian follicle population. The aim of the study was to investigate the effect of different anethole concentrations on the in vitro culture of caprine preantral follicles enclosed in ovarian tissue. Randomized ovarian fragments were fixed immediately (non-cultured treatment) or distributed into five treatments: α-MEM+ (cultured control), α-MEM+ supplemented with ascorbic acid at 50 μg/mL (AA), and anethole at 30 (AN30), 300 (AN300), or 2000 µg/mL (AN2000), for 1 or 7 days. After 7 days of culture, a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Anethole; ROS; Primordial follicle; Caprine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800602

Plasmatic ADAMTS-13 metalloprotease and von Willebrand factor in children with cyanotic congenital heart disease
Provedor de dados: 56
Autores: Soares,R.P.S.; Bydlowski,S.P.; Nascimento,N.M.; Thomaz,A.M.; Bastos,E.N.M.; Lopes,A.A.
Changes in plasma von Willebrand factor concentration (VWF:Ag) and ADAMTS-13 activity (the metalloprotease that cleaves VWF physiologically) have been reported in several cardiovascular disorders with prognostic implications. We therefore determined the level of these proteins in the plasma of children with cyanotic congenital heart disease (CCHD) undergoing surgical treatment. Forty-eight children were enrolled (age 0.83 to 7.58 years). Measurements were performed at baseline and 48 h after surgery. ELISA, collagen-binding assays and Western blotting were used to estimate antigenic and biological activities, and proteolysis of VWF multimers. Preoperatively, VWF:Ag and ADAMTS-13 activity were decreased (65 and 71% of normal levels considered as 113...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ADAMTS-13; Von Willebrand factor; Congenital heart disease; Thrombosis; Cardiac surgery.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000400375

Effect of metabolic control on interferon-gamma and interleukin-10 production by peripheral blood mononuclear cells from type 1 and type 2 diabetic patients
Provedor de dados: 56
Autores: Foss-Freitas,M.C.; Foss,N.T.; Donadi,E.A.; Foss,M.C.
The objective of the present study was to evaluate the production of cytokines, interferon-g (INF-g) and interleukin-10 (IL-10), in cultures of peripheral blood mononuclear cells (PBMC) from type 1 and type 2 diabetic patients and to correlate it with inadequate and adequate metabolic control. We studied 11 type 1 and 13 type 2 diabetic patients and 21 healthy individuals divided into two groups (N = 11 and 10) paired by sex and age with type 1 and type 2 diabetic patients. The PBMC cultures were stimulated with concanavalin-A to measure INF-g and IL-10 supernatant concentration by ELISA. For patients with inadequate metabolic control, the cultures were performed on the first day of hospitalization and again after intensive treatment to achieve adequate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 1 diabetes mellitus; Type 2 diabetes mellitus; Cytokines; Interferon-gamma; Interleukin-10; Metabolic control.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500010

ADME studies and preliminary safety pharmacology of LDT5, a lead compound for the treatment of benign prostatic hyperplasia
Provedor de dados: 56
Autores: Noël,F.; Nascimento-Viana,J.B.; Romeiro,L.A.S.; Silva,R.O.; Lemes,L.F.N.; Oliveira,A.S.; Giorno,T.B.S.; Fernandes,P.D.; Silva,C.L.M.
This study aimed to estimate the absorption, distribution, metabolism and excretion (ADME) properties and safety of LDT5, a lead compound for oral treatment of benign prostatic hyperplasia that has previously been characterized as a multi-target antagonist of α1A-, α1D-adrenoceptors and 5-HT1A receptors. The preclinical characterization of this compound comprised the evaluation of its in vitro properties, including plasma, microsomal and hepatocytes stability, cytochrome P450 metabolism and inhibition, plasma protein binding, and permeability using MDCK-MDR1 cells. De-risking and preliminary safety pharmacology assays were performed through screening of 44 off-target receptors and in vivo tests in mice (rota-rod and single dose toxicity). LDT5 is stable in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Benign prostatic hyperplasia; ADME; Safety; Permeability; CYP; Preclinical development.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200603

Development of fetal nicotine and muscarinic receptors in utero
Provedor de dados: 56
Autores: Mao,C.; Lv,J.; Li,H.; Chen,Y.; Wu,J.; Xu,Z.
The role of acetylcholine in the central and peripheral nervous systems is well established in adults. Cholinergic modulation of vascular functions and body fluid balance has been extensively studied. In the embryo-fetus, cholinergic receptors are widespread in the peripheral and central systems, including smooth muscle and the epithelial lining of the cardiovascular, digestive, and urinary systems, as well as in the brain. Fetal nicotine and muscarinic receptors develop in a pattern (e.g., amount and distribution) related to gestational periods. Cholinergic mechanisms have been found to be relatively intact and functional in the control of vascular homeostasis during fetal life in utero at least during the last third of gestation. This review focuses on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylcholine; Muscarinic/nicotinic receptors; Fetus; Swallowing; Vascular homeostasis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500019

Spectral and symbolic analysis of the effect of gender and postural change on cardiac autonomic modulation in healthy elderly subjects
Provedor de dados: 56
Autores: Perseguini,N.M.; Takahashi,A.C.M.; Rebelatto,J.R.; Silva,E.; Borghi-Silva,A.; Porta,A.; Montano,N.; Catai,A.M.
The objective of this study was to use linear and non-linear methods to investigate cardiac autonomic modulation in healthy elderly men and women in response to a postural change from the supine to the standing position. Fourteen men (66.1 ± 3.5 years) and 10 women (65.3 ± 3.3 years) were evaluated. Beat-to-beat heart rate was recorded in the supine and standing positions. Heart rate variability was studied by spectral analysis, including both low (LFnu-cardiac sympathetic modulation (CSM) indicator) and high (HFnu-cardiac vagal modulation (CVM) indicator) frequencies in normalized units as well as the low frequency/high frequency (LF/HF) ratio. Symbolic analysis was performed using the following indexes: 0V% (CSM indicator), 1V% (CSM and CVM indicators),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart rate variability; Autonomic nervous system; Symbolic analysis; Spectral analysis; Elderly; Gender.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100005

Photocytotoxicity of a 5-nitrofuran-ethenyl-quinoline antiseptic (Quinifuryl) to P388 mouse leukemia cells
Provedor de dados: 56
Autores: Daghastanli,N.A.; Degterev,I.A.; Tedesco,A.C.; Borissevitch,I.E.
Quinifuryl (MW 449.52), 2-(5'-nitro-2'-furanyl)ethenyl-4-{N-[4'-(N,N-diethylamino)-1'-methylbutyl]carbamoyl} quinoline, is a water soluble representative of a family of 5-nitrofuran-ethenyl-quinoline drugs which has been shown to be highly toxic to various lines of transformed cells in the dark. In the present study, the toxicity of Quinifuryl to P388 mouse leukemia cells was compared in the dark and under illumination with visible light (390-500 nm). Illumination of water solutions of Quinifuryl (at concentrations ranging from 0.09 to 9.0 µg/ml) in the presence of P388 cells resulted in its photodecomposition and was accompanied by elevated cytotoxicity. A significant capacity to kill P388 cells was detected at a drug concentration as low as 0.09 µg/ml....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phototoxicity; Nitrofurane derivatives; Quinifuryl; Oxygen and nitrogen active species.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200013

Morphometry and acetylcholinesterase activity of the myenteric plexus of the wild mouse Calomys callosus
Provedor de dados: 56
The myenteric plexus of the digestive tract of the wild mouse Calomys callosus was examined using a histochemical method that selectively stains nerve cells, and the acetylcholinesterase (AChE) histochemical technique in whole-mount preparations. Neuronal density was 1,500 ± 116 neurons/cm2 (mean ± SEM) in the esophagus, 8,900 ± 1,518 in the stomach, 9,000 ± 711 in the jejunum and 13,100 ± 2,089 in the colon. The difference in neuronal density between the esophagus and other regions was statistically significant. The neuron profile area ranged from 45 to 1,100 µm2. The difference in nerve cell size between the jejunum and other regions was statistically significant. AChE-positive nerve fibers were distributed within the myenteric plexus which is formed by...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myenteric plexus; Wild mouse; Calomys callosus; Morphometry; Histochemistry.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500009

Effects of nanoparticles of hydroxy-aluminum phthalocyanine on markers of liver injury and glucose metabolism in diabetic mice
Provedor de dados: 56
Autores: Melo,M.A.B.; Caetano,W.; Oliveira,E.L.; Barbosa,P.M.; Rando,A.L.B.; Pedrosa,M.M.D.; Godoi,V.A.F.
Photodynamic therapy, by reducing pain and inflammation and promoting the proliferation of healthy cells, can be used to treat recurrent lesions, such as diabetic foot ulcers. Studies using the photosensitizer phthalocyanine, together with the nanostructured copolymeric matrix of Pluronic® and Carbopol® for the treatment of diabetic foot ulcers and leishmaniosis lesions, are showing promising outcomes. Despite their topical or subcutaneous administration, these molecules are absorbed and their systemic effects are unknown. Therefore, we investigated the effect of the subcutaneous administration of the hydroxy-aluminum phthalocyanine hydrogel without illumination on systemic parameters, markers of liver injury, and liver energy metabolism in type 1 diabetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydroxy-aluminum phthalocyanine; Hydrogel; Liver; Type 1 diabetes; Mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100609

ClC-5 chloride channel and kidney stones: what is the link?
Provedor de dados: 56
Autores: Silva,I.V.; Morales,M.M.; Lopes,A.G.
Nephrolithiasis is one of the most common diseases in the Western world. The disease manifests itself with intensive pain, sporadic infections, and, sometimes, renal failure. The symptoms are due to the appearance of urinary stones (calculi) which are formed mainly by calcium salts. These calcium salts precipitate in the renal papillae and/or within the collecting ducts. Inherited forms of nephrolithiasis related to chromosome X (X-linked hypercalciuric nephrolithiasis or XLN) have been recently described. Hypercalciuria, nephrocalcinosis, and male predominance are the major characteristics of these diseases. The gene responsible for the XLN forms of kidney stones was cloned and characterized as a chloride channel called ClC-5. The ClC-5 chloride channel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nephrolithiasis; Dent's disease; Calcium reabsorption; Chloride channels.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300004

Interferon-alpha receptor 1 mRNA expression in peripheral blood mononuclear cells is associated with response to interferon-alpha therapy of patients with chronic hepatitis C
Provedor de dados: 56
Autores: Massirer,K.B.; Hirata,M.H.; Silva,A.E.B.; Ferraz,M.L.G.; Nguyen,N.Y.; Hirata,R.D.C.
Interferon (IFN)-alpha receptor mRNA expression in liver of patients with chronic hepatitis C has been shown to be a response to IFN-alpha therapy. The objective of the present study was to determine whether the expression of mRNA for subunit 1 of the IFN-alpha receptor (IFNAR1) in peripheral blood mononuclear cells (PBMC) is associated with the response to IFN-alpha in patients with chronic hepatitis C. Thirty patients with positive anti-HCV and HCV-RNA, and abnormal levels of alanine aminotransferase in serum were selected and treated with IFN-alpha2b for one year. Those with HBV or HIV infection, or using alcohol were not included. Thirteen discontinued the treatment and were not evaluated. The IFN-alpha response was monitored on the basis of alanine...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: IFNAR1-mRNA expression; Interferon-alpha therapy; Chronic hepatitis C; Interferon-alpha receptor; Peripheral blood mononuclear cells; Reverse transcription- polymerase chain reaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500003

Expression, production, and renaturation of a functional single-chain variable antibody fragment (scFv) against human ICAM-1
Provedor de dados: 56
Autores: Sun,H.; Wu,G.M.; Chen,Y.Y.; Tian,Y.; Yue,Y.H.; Zhang,G.L.
Intercellular adhesion molecule-1 (ICAM-1) is an important factor in the progression of inflammatory responses in vivo. To develop a new anti-inflammatory drug to block the biological activity of ICAM-1, we produced a monoclonal antibody (Ka=4.19×10−8 M) against human ICAM-1. The anti-ICAM-1 single-chain variable antibody fragment (scFv) was expressed at a high level as inclusion bodies in Escherichia coli. We refolded the scFv (Ka=2.35×10−7 M) by ion-exchange chromatography, dialysis, and dilution. The results showed that column chromatography refolding by high-performance Q Sepharose had remarkable advantages over conventional dilution and dialysis methods. Furthermore, the anti-ICAM-1 scFv yield of about 60 mg/L was higher with this method. The purity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intercellular adhesion molecule-1; Single-chain variable antibody fragment; Expression; Purification; Renaturation; Biological activity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700540

Discrepancies between clinical diagnoses and autopsy findings
Provedor de dados: 56
Autores: Coradazzi,A.L.; Morganti,A.L.C.; Montenegro,M.R.G.
Autopsy examination is considered to be an essential element for medical auditing and teaching. Despite the significant progress in diagnostic procedures, autopsy has not always confirmed the clinical diagnosis. In the present study, we compared the diagnosis recorded on medical charts with reports of 96 autopsies performed at the University Teaching Hospital of the Faculdade de Medicina de Botucatu, Botucatu, SP, Brazil, between 1975 and 1982, and of 156 autopsies performed at the same institution between 1992 and 1996. The clinical diagnosis of the basic cause of death was confirmed at autopsy in 77% of cases. The percent confirmation fell to 60% when the immediate terminal cause of death was considered, and in 25% of cases, the terminal cause was only...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autopsy diagnosis; Clinical diagnosis; Iatrogenic injuries.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300014

Association of Bartonella spp bacteremia with Chagas cardiomyopathy, endocarditis and arrythmias in patients from South America
Provedor de dados: 56
Autores: Corrêa,F.G.; Pontes,C.L.S.; Verzola,R.M.M.; Mateos,J.C.P.; Velho,P.E.N.F.; Schijman,A.G.; Selistre-de-Araujo,H.S.
Infection with Bartonella spp may cause cardiac arrhythmias, myocarditis and endocarditis in humans. The aim of the present study was to evaluate a possible association between Bartonella spp bacteremia and endocarditis, arrhythmia and Chagas cardiomyopathy in patients from Brazil and Argentina. We screened for the presence of bacterial 16S rRNA in human blood by PCR using oligonucleotides to amplify a 185-bp bacterial DNA fragment. Blood samples were taken from four groups of subjects in Brazil and Argentina: i) control patients without clinical disease, ii) patients with negative blood-culture endocarditis, iii) patients with arrhythmias, and iv) patients with chronic Chagas cardiomyopathy. PCR products were analyzed on 1.5% agarose gel to visualize the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human; PCR; Bartonella spp; Chagas disease; Endocarditis; Arrhythmia.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700012

Effect of hydroxyurea on G gamma chain fetal hemoglobin synthesis by sickle-cell disease patients
Provedor de dados: 56
Autores: Teixeira,S.M.; Cortellazzi,L.C.; Grotto,H.Z.W.
Hydroxyurea is used for sickle-cell disease patients in order to increase fetal hemoglobin synthesis and consequently decrease the severity of pain episodes. Fetal hemoglobin, which is formed by gamma-globin chains A and G, is present in a constant composition throughout fetal development: about 75% of Ggamma and 25% of Agamma. In contrast, adult red cells contain about 40% of Ggamma and 60% of Agamma. In the present study, we analyzed the effect of hydroxyurea induction on the gamma chain composition of fetal hemoglobin in 31 sickle-cell disease patients treated with hydroxyurea. The control group was composed of 30 sickle-cell disease patients not treated with hydroxyurea in clinical steady state. The patients were older than 13 years and were not...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gamma chain; Fetal hemoglobin; Hydroxyurea; Sickle-cell disease.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000002

Methodological approaches to planar and volumetric scintigraphic imaging of small volume targets with high spatial resolution and sensitivity
Provedor de dados: 56
Autores: Mejia,J.; Galvis-Alonso,O.Y.; Braga,J.; Corrêa,R.; Leite,J.P.; Simões,M.V.
Single-photon emission computed tomography (SPECT) is a non-invasive imaging technique, which provides information reporting the functional states of tissues. SPECT imaging has been used as a diagnostic tool in several human disorders and can be used in animal models of diseases for physiopathological, genomic and drug discovery studies. However, most of the experimental models used in research involve rodents, which are at least one order of magnitude smaller in linear dimensions than man. Consequently, images of targets obtained with conventional gamma-cameras and collimators have poor spatial resolution and statistical quality. We review the methodological approaches developed in recent years in order to obtain images of small targets with good spatial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Small animal SPECT; Multipinhole collimators; Coded masks.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800001

Substance P immunoreactivity in the lumbar spinal cord of the turtle Trachemys dorbigni following peripheral nerve injury
Provedor de dados: 56
Autores: Partata,W.A.; Krepsky,A.M.R.; Xavier,L.L.; Marques,M.; Achaval,M.
Immunoreactive substance P was investigated in turtle lumbar spinal cord after sciatic nerve transection. In control animals immunoreactive fibers were densest in synaptic field Ia, where the longest axons invaded synaptic field III. Positive neuronal bodies were identified in the lateral column of the dorsal horn and substance P immunoreactive varicosities were observed in the ventral horn, in close relationship with presumed motoneurons. Other varicosities appeared in the lateral and anterior funiculi. After axotomy, substance P immunoreactive fibers were reduced slightly on the side of the lesion, which was located in long fibers that invaded synaptic field III and in the varicosities of the lateral and anterior funiculus. The changes were observed at 7...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Substance P immunoreactivity; Axonal injury; Spinal cord; Turtle; Trachemys dorbigni.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400015

T-cell lymphoma with rhabdomyolysis: case report and literature review
Provedor de dados: 56
Autores: Ouyang,Zhao-Zhong; Peng,Ting-Sheng; Cao,Qing-Hua; Ouyang,Yin; Li,Jun-Xun; Wei,Qiu-Xiang; Zhan,Hong.
Rhabdomyolysis refers to the destruction or disintegration of striated muscles. This syndrome is characterized by muscle breakdown and necrosis, resulting in the leakage of intracellular muscle constituents into the circulation and extracellular fluid. We report a rare case of rhabdomyolysis complicating multi-organ failure caused by T-cell lymphoma in a 32-year-old woman. The final diagnosis was rhabdomyolysis caused by peripheral T-cell lymphoma based on bone marrow aspirate and biopsy.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Rhabdomyolysis; Multiple organ failure; T-cell lymphoma; Hemophagocytic lymphohistiocytosis; Case report.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100501

Kidney health for everyone everywhere – from prevention to detection and equitable access to care
Provedor de dados: 56
Autores: Li,P. Kam-Tao; Garcia-Garcia,G.; Lui,Siu-Fai; Andreoli,S.; Fung,W. Wing-Shing; Hradsky,A.; Kumaraswami,L.; Liakopoulos,V.; Rakhimova,Z.; Saadi,G.; Strani,L.; Ulasi,I.; Kalantar-Zadeh,K.
The global burden of chronic kidney disease (CKD) is rapidly increasing with a projection of becoming the 5th most common cause of years of life lost globally by 2040. CKD is a major cause of catastrophic health expenditure. The costs of dialysis and transplantation consume up to 3% of the annual healthcare budget in high-income countries. However, the onset and progression of CKD is often preventable. In 2020, the World Kidney Day campaign highlights the importance of preventive interventions – be it primary, secondary, or tertiary. This article focuses on outlining and analyzing measures that can be implemented in every country to promote and advance CKD prevention. Primary prevention of kidney disease should focus on the modification of risk factors and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Kidney diseases; Prevention; Detection; Awareness.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300101

Modulation of the prostaglandin-endoperoxide synthase 2 gene expression by variant haplotypes: influence of the 3′-untranslated region
Provedor de dados: 56
Autores: Piranda,D.N.; Abreu,R.B.V.; Freitas-Alves,D.R.; de Carvalho,M.A.; Vianna-Jorge,R.
The inducible inflammatory enzyme cycloxigenase-2 is up-regulated in cancer, and favors tumor progression. Cycloxigenase-2 is encoded by the prostaglandin-endoperoxide synthase 2 (PTGS2) gene, which presents sequence variations in the promoter region (PR) and in the 3′-untranslated region (3′-UTR). Different PR (rs689465, rs689466, rs20417) and 3′-UTR (rs5275) variants were generated by site-directed mutagenesis, and combined in haplotypes to access expression levels using a reporter system (luciferase) in human cells (MCF-7 and HEK293FT). Luciferase activity did not differ significantly among PTGS2 PR constructs, except for pAAC (containing variant allele rs20417 C), with 40% less activity than pAAG (wild-type sequence) in MCF-7 cells (P&lt;0.01). Despite...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COX-2; PTGS2; Haplotypes; Gene expression; MCF-7; HEK293FT.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200602

Inhibitory effect of high [Mg2+] on the vasopressin-stimulated hydroosmotic permeability of the isolated perfused cortical collecting duct
Provedor de dados: 56
Autores: Falkenstein,D.; Ribeiro,C.A.L.; Figueiredo,J.F.
High magnesium concentration inhibits the effect of arginine vasopressin (AVP) on smooth muscle contraction and platelet aggregation and also influences hepatocyte AVP receptor binding. The aim of this study was to determine the role of magnesium concentration [Mg2+] in AVP-stimulated water transport in the kidney collecting duct. The effect of low and high peritubular [Mg2+] on the AVP-stimulated osmotic water permeability coefficient (Pf) was evaluated in the isolated perfused rabbit cortical collecting duct (CCD). Control tubules bathed and perfused with standard Ringer bicarbonate solution containing 1 mM Mg2+ presented a Pf of 223.9 ± 27.2 µm/s. When Mg2+ was not added to the bathing solution, an increase in the AVP-stimulated Pf to 363.1 ± 57.2 µm/s...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cortical collecting ducts; Magnesium; Arginine vasopressin; Osmotic water permeability.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800016

CD19+ B cell subsets in the peripheral blood and skin lesions of psoriasis patients and their correlations with disease severity
Provedor de dados: 56
Autores: Lu,J.; Ding,Y.; Yi,X.; Zheng,J.
T lymphocytes are important in the pathogenesis of psoriasis, and increasing evidence indicates that B cells also play an important role. The mechanisms of action, however, remain unclear. We evaluated the ratios of CD19+ B cells in peripheral blood mononuclear cells (PBMCs) from 157 patients with psoriasis (65 patients with psoriasis vulgaris, 32 patients with erythrodermic psoriasis, 30 patients with arthropathic psoriasis, and 30 patients with pustular psoriasis) and 35 healthy controls (HCs). Ratios of CD19+ B cells in skin lesions were compared with non-lesions in 7 erythrodermic psoriasis patients. The Psoriasis Area Severity Index (PASI) was used to measure disease severity. CD19+ B cell ratios in PBMCs from psoriasis vulgaris (at both the active...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psoriasis; B-lymphocytes; B-lymphocyte subsets; Psoriasis Area Severity Index (PASI); Flow cytometry.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900706

Asthma: where is it going?
Provedor de dados: 56
Autores: Faffe,D.S.
Asthma is characterized by reversible airway obstruction, airway hyperresponsiveness, and airway inflammation. Although our understanding of its pathophysiological mechanisms continues to evolve, the relative contributions of airway hyperresponsiveness and inflammation are still debated. The first mechanism identified as important for asthma was bronchial hyperresponsiveness. In a second step, asthma was recognized also as an inflammatory disease, with chronic inflammation inducing structural changes or remodeling. However, persistence of airway dysfunction despite inflammatory control is observed in chronic severe asthma of both adults and children. More recently, a potential role for epithelial-mesenchymal communication or transition is emerging, with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Epithelial-mesenchymal unit; Bronchial hyperresponsiveness; Remodeling.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900001

Influence of season and pollution on the antioxidant defenses of the cichlid fish acará (Geophagus brasiliensis)
Provedor de dados: 56
Autores: Wilhelm Filho,D.; Torres,M.A.; Tribess,T.B.; Pedrosa,R.C.; Soares,C.H.L.
The livers of Geophagus brasiliensis collected from both a non-polluted site and a polluted site were analyzed for different antioxidant defenses, O2 consumption, thiobarbituric acid-reactive substance (TBARS) levels, and histological damage. Compared to controls (116.6 ± 26.1 nmol g-1), TBARS levels were enhanced at the polluted site (284.2 ± 25.6 nmol g-1), as also was oxygen consumption (86.6 ± 11.3 and 128.5 ± 9.8 µmol O2 min-1 g-1, respectively). With respect to enzymatic antioxidants, increased catalase activities (8.7 ± 1.3 and 29.2 ± 2.4 mmol min-1 g-1, respectively), unchanged superoxide dismutase activities (767.2 ± 113.3 and 563.3 ± 70.2 U g-1, respectively), and diminished glutathione S-transferase activities (29.0 ± 3.2 and 14.9 ± 3.2 µmol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxyradicals; Antioxidant defenses; Fish; Acará; Season; Pollution; Adaptation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600004

Immunoexpression of cyclooxygenase-1 and -2 in ulcerative colitis
Provedor de dados: 56
Autores: Paiotti,A.P.R.; Artigiani Neto,R.; Forones,N.M.; Oshima,C.T.F.; Miszputen,S.J.; Franco,M.
Ulcerative colitis (UC) is a disease of the colon and rectum characterized by a nonspecific chronic inflammation mediated by the concerted response of cellular and humoral events. Prostaglandins are synthesized by cyclooxygenase (COX)-1 and -2 and exhibit both pro- and anti-inflammatory activity. To evaluate COX-1 and COX-2 immunoexpression in 42 cases of UC and to correlate it with clinicopathological parameters, COX-1 and COX-2 expression was investigated by the immunohistochemistry method. Only patients with all pertinent clinical and evolutive data as well as with adequate biopsy material were included in the study. Fifteen samples of colorectal adenocarcinoma and 14 of large bowel with no histological changes were used for positive and negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ulcerative colitis; Cyclooxygenases; Prostaglandins; Immunohistochemistry.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700004

Effect of cardiopulmonary bypass on the pharmacokinetics of propranolol and atenolol
Provedor de dados: 56
Autores: Carmona,M.J.C.; Pereira,V.A.; Malbouisson,L.M.S.; Auler Jr.,J.O.C.; Santos,S.R.C.J.
The pharmacokinetics of some β-blockers are altered by cardiopulmonary bypass (CPB). The objective of this study was to compare the effect of coronary artery bypass graft (CABG) surgery employing CPB on the pharmacokinetics of propranolol and atenolol. We studied patients receiving oral propranolol with doses ranging from 80 to 240 mg (N = 11) or atenolol with doses ranging from 25 to 100 mg (N = 8) in the pre- and postoperative period of CABG with moderately hypothermic CPB (32°C). On the day before and on the first day after surgery, blood samples were collected before β-blocker administration and every 2 h thereafter. Plasma levels were determined using high-performance liquid chromatography and data were treated by pharmacokinetics-modelling....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propranolol; Atenolol; Pharmacokinetics; Coronary artery bypass grafting surgery; Cardiopulmonary bypass.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600016

Agonistic activity of tamoxifen, a selective estrogen-receptor modulator (SERM), on arthritic ovariectomized mice
Provedor de dados: 56
Autores: Silva,L.A.S.; Felix,F.B.; Araujo,J.M.D.; Souza,E.V.; Camargo,E.A.; Grespan,R.
Arthritis is positively associated with the decline of sex hormones, especially estrogen. Tamoxifen (TMX) is a selective estrogen receptor modulator, possessing agonist or antagonistic activity in different tissues. Thus, the objective of this study was to investigate the effect of TMX on the zymosan-induced arthritis model. Female Swiss normal and ovariectomized (OVX) mice were divided into groups and treated for five days with TMX (0.3, 0.9 or 2.7 mg/kg) or 17-β-estradiol (E2, 50 µg/kg). On the fifth day, arthritis was induced and 4 h later, leukocyte migration into joint cavities was evaluated. The neutrophil migration in OVX animals, but not in normal mice, treated with TMX (all tested doses) was significantly decreased compared with mice that received...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arthritis Tamoxifen Neutrophils; Estrogen Ovariectomy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100614

Low-frequency fatigue at maximal and submaximal muscle contractions
Provedor de dados: 56
Autores: Baptista,R.R.; Scheeren,E.M.; Macintosh,B.R.; Vaz,M.A.
Skeletal muscle force production following repetitive contractions is preferentially reduced when muscle is evaluated with low-frequency stimulation. This selective impairment in force generation is called low-frequency fatigue (LFF) and could be dependent on the contraction type. The purpose of this study was to compare LFF after concentric and eccentric maximal and submaximal contractions of knee extensor muscles. Ten healthy male subjects (age: 23.6 ± 4.2 years; weight: 73.8 ± 7.7 kg; height: 1.79 ± 0.05 m) executed maximal voluntary contractions that were measured before a fatigue test (pre-exercise), immediately after (after-exercise) and after 1 h of recovery (after-recovery). The fatigue test consisted of 60 maximal (100%) or submaximal (40%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eccentric contractions; Concentric contractions; Knee extensor torque; Electrical stimulation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400011

Mineralocorticoid modulation of central angiotensin-induced neuronal activity, water intake and sodium appetite
Provedor de dados: 56
Autores: Thornton,S.N.; Omouessi,S.T.; Falconetti,C.
Central angiotensin II (AngII) stimulates water and salt solution intake. Pretreatment with low-dose mineralocorticoid (DOCA) enhances this AngII-induced intake of salt solutions (the synergy theory) in Wistar and Sprague Dawley rats but not in Fischer rats. This response is mediated via the AT-1 receptor. Electrophysiological experiments using iontophoretic application of AngII and the AT-1 receptor-specific non-peptide antagonist losartan showed excitation of neurons in the preoptic/medial septum region of urethane-anesthetized male Wistar rats. DOCA pretreatment further enhances this neuronal excitation in response to AngII and reduces the responses to losartan. This generated the hypothesis that DOCA-enhanced AngII-induced neuronal excitation is the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aldosterone; Rat; AT-1; AT-2; Iontophoresis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500014

Racial inequalities and perinatal health in the southeast region of Brazil
Provedor de dados: 56
Autores: Silva,L.M.; Silva,R.A.; Silva,A.A.M.; Bettiol,H.; Barbieri,M.A.
Few studies are available about racial inequalities in perinatal health in Brazil and little is known about whether the existing inequality is due to socioeconomic factors or to racial discrimination per se. Data regarding the Ribeirão Preto birth cohort, Brazil, whose mothers were interviewed from June 1, 1978 to May 31, 1979 were used to answer these questions. The perinatal factors were obtained from the birth questionnaire and the ethnic data were obtained from 2063 participants asked about self-reported skin color at early adulthood (23-25 years of age) in 2002/2004. Mothers of mulatto and black children had higher rates of low schooling (£4 years, 27.2 and 38.0%) and lower family income (£1 minimum wage, 28.6 and 30.4%). Mothers aged less than 20...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethnic-racial discrimination; Prenatal care; Preterm birth; Low birth weight; Perinatal health; Cesarean section.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900005

Studies on the intrinsic nervous system of the wild rodent Calomys callosus digestive tract. II: The submucous plexus
Provedor de dados: 56
Autores: Souza,N.B.; Liberti,E.A.; De-Souza,R.R.
The submucous plexus of the normal small and large intestine of Calomys callosus was studied by NADH and AChE histochemical techniques and by transmission and scanning electron microscopy. The plexus contains (mean ± SD) 7,488 ± 293 neurons/cm2 in the duodenum, 5,611 ± 836 in the jejunum, 2,741 ± 360 in the ileum, 3,067 ± 179 in the cecum, and 3,817 ± 256 in the proximal colon. No ganglia or nerve cell bodies were seen in the esophagus, stomach, distal colon or rectum. The neurons are pear-shaped with a round or oval nucleus and the neuronal cell profile areas were larger in the large intestine than in the small intestine. Most of the neurons display intense AChE activity in the cytoplasm. AChE-positive nerve fibers are present in a primary meshwork of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Submucous plexus; Wild mouse; Morphometry; Histochemistry; Ultrastructure; Calomys callosus.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500007

Nucleotide-sugar transporters: structure, function and roles in vivo
Provedor de dados: 56
Autores: Handford,M.; Rodriguez-Furlán,C.; Orellana,A.
The glycosylation of glycoconjugates and the biosynthesis of polysaccharides depend on nucleotide-sugars which are the substrates for glycosyltransferases. A large proportion of these enzymes are located within the lumen of the Golgi apparatus as well as the endoplasmic reticulum, while many of the nucleotide-sugars are synthesized in the cytosol. Thus, nucleotide-sugars are translocated from the cytosol to the lumen of the Golgi apparatus and endoplasmic reticulum by multiple spanning domain proteins known as nucleotide-sugar transporters (NSTs). These proteins were first identified biochemically and some of them were cloned by complementation of mutants. Genome and expressed sequence tag sequencing allowed the identification of a number of sequences that...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glycoconjugates; Glycosyltransferases; Golgi apparatus; Nucleotide-sugars; Nucleotide-sugar transporters.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900002

Monosodium glutamate (MSG)-obese rats develop glucose intolerance and insulin resistance to peripheral glucose uptake
Provedor de dados: 56
Different levels of insulin sensitivity have been described in several animal models of obesity as well as in humans. Monosodium glutamate (MSG)-obese mice were considered not to be insulin resistant from data obtained in oral glucose tolerance tests. To reevaluate insulin resistance by the intravenous glucose tolerance test (IVGTT) and by the clamp technique, newborn male Wistar rats (N = 20) were injected 5 times, every other day, with 4 g/kg MSG (N = 10) or saline (control; N = 10) during the first 10 days of age. At 3 months, the IVGTT was performed by injecting glucose (0.75 g/kg) through the jugular vein into freely moving rats. During euglycemic clamping plasma insulin levels were increased by infusing 3 mU . kg-1 . min-1 of regular insulin until a...
Palavras-chave: Monosodium glutamate; Insulin resistance; Obesity; Clamp technique.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500016

Modulation of staphylokinase-dependent plasminogen activation by mono- and divalent ions
Provedor de dados: 56
Autores: Yarzábal,A.; Serrano,R.L.; Puig,J.
The effect of several ions (Cl-, Na+, K+, Ca2+) on the rate of plasminogen (Pg) activation by recombinant staphylokinase (rSTA) is reported. Both monovalent and divalent ions affect the rate at which Pg is activated by rSTA, in a concentration-dependent manner (range 0-100 mM). In almost all cases, a decrease of the initial velocity of activation was observed. Cl- showed the most striking inhibitory effect at low concentrations (64% at 10 mM). However, in the presence of a fibrin surface, this inhibition was attenuated to 38%. Surprisingly, 10 mM Ca2+ enhanced the Pg activation rate 21% when a polymerized fibrin matrix was present. These data support the idea that ions can modulate the rate of Pg activation through a mechanism that may be associated with...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Staphylokinase; Plasminogen activation; Fibrinolysis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100005

Infection with human papillomaviruses of sexual partners of women having cervical intraepithelial neoplasia
Provedor de dados: 56
Autores: Rombaldi,R.L.; Serafini,E.P.; Villa,L.L.; Vanni,A.C.; Baréa,F.; Frassini,R.; Xavier,M.; Paesi,S.
Epidemiological studies show that human papillomaviruses (HPV) are strongly related to cervical cancer and cervical intraepithelial neoplasias (CIN). Unlike the case for women, there are no consistent data on the natural history of HPV in the male population even though these viruses are prevalent in males. We carried out a prospective study to assess the prevalence of HPV in males as well as the factors that determine such infections in 99 male sexual partners of women with CIN. The genitalia of the males were physically examined and subjected to peniscopy for the collection of scrapings which were subjected to the polymerase chain reaction and restriction fragment length polymorphism to detect HPV. Of the 99 males sampled, 54 (54.5%) were positive for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cervical intraepithelial neoplasia; Epidemiology; Human papillomaviruses; Polymerase chain reaction.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200003

Pharmacological study of the mechanisms involved in the vasodilator effect produced by the acute application of triiodothyronine to rat aortic rings
Provedor de dados: 56
Autores: Lozano-Cuenca,J.; López-Canales,O.A.; Aguilar-Carrasco,J.C.; Villagrana-Zesati,J.R.; López-Mayorga,R.M.; Castillo-Henkel,E.F.; López-Canales,J.S.
A relationship between thyroid hormones and the cardiovascular system has been well established in the literature. The present in vitro study aimed to investigate the mechanisms involved in the vasodilator effect produced by the acute application of 10-8–10-4 M triiodothyronine (T3) to isolated rat aortic rings. Thoracic aortic rings from 80 adult male Wistar rats were isolated and mounted in tissue chambers filled with Krebs-Henseleit bicarbonate buffer in order to analyze the influence of endothelial tissue, inhibitors and blockers on the vascular effect produced by T3. T3 induced a vasorelaxant response in phenylephrine-precontracted rat aortic rings at higher concentrations (10-4.5–10-4.0 M). This outcome was unaffected by 3.1×10-7 M glibenclamide,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triiodothyronine; Rat aorta; Vasorelaxation; NO-cGMP/PKG pathway; K+ channels.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800604

Relationship between aerobic capacity and pelvic floor muscles function: a cross-sectional study
Provedor de dados: 56
Autores: Jürgensen,S.P.; Borghi-Silva,A.; Bastos,A.M.F.G.; Correia,G.N.; Pereira-Baldon,V.S.; Cabiddu,R.; Catai,A.M.; Driusso,P.
The objective of this study was to evaluate the relationship between aerobic capacity and pelvic floor muscles (PFM) function in adult women. Women aged 18 or over and without urinary dysfunction or other chronic diseases were eligible to participate. They completed the habitual physical activity (HPA) questionnaire, underwent a PFM functional evaluation by palpation and perineometry, and performed a submaximal (between 75 and 85% of maximum heart rate) cardiopulmonary exercise (CPX) test to determine the ventilatory anaerobic threshold (VAT). Forty-one women were included (35±16 years, 75% physically active, 17% very active, and 8% sedentary and 17% presented grade 1 PFM contraction, 31.8% grade 2, 26.8% grade 3, and 24.4% grade 4, according to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional capacity; Muscle strength; Women; Physical activity; Oxygen consumption.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100613

Effects of estrogen on the vascular system
Provedor de dados: 56
Autores: Tostes,R.C.; Nigro,D.; Fortes,Z.B.; Carvalho,M.H.C.
The cardiovascular protective actions of estrogen are partially mediated by a direct effect on the vessel wall. Estrogen is active both on vascular smooth muscle and endothelial cells where functionally competent estrogen receptors have been identified. Estrogen administration promotes vasodilation in humans and in experimental animals, in part by stimulating prostacyclin and nitric oxide synthesis, as well as by decreasing the production of vasoconstrictor agents such as cyclooxygenase-derived products, reactive oxygen species, angiotensin II, and endothelin-1. In vitro, estrogen exerts a direct inhibitory effect on smooth muscle by activating potassium efflux and by inhibiting calcium influx. In addition, estrogen inhibits vascular smooth muscle cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sex hormones; Estrogen; Vascular smooth muscle; Endothelium nitric oxide; Endothelium-derived hyperpolarizing factor; Angiotensin; Endothelin-1; Calcium channels; Potassium channels.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900002

Effect of maternal hydration on the increase of amniotic fluid index
Provedor de dados: 56
Autores: Borges,V.T.M.; Rososchansky,J.; Abbade,J.F.; Dias,A.; Peraçoli,J.C.; Rudge,M.V.C.
The objective of this study was to determine the effect of maternal hydration with oral isotonic solution and water on the amniotic fluid (AF) index of women with normohydramnios. Women with a normal AF index and gestational age between 33 and 36 weeks without maternal complications were randomized into three groups [isotonic solution (Gatorade®), water, control]. The isotonic solution and water groups were instructed to drink 1.5 L of the respective solution and the control group was instructed to drink 200 mL water over a period of 2 to 4 h. AF index was measured before and after hydration by Doppler ultrasonography. The investigator performing the AF index measurement was blind to the subject’s group. Ninety-nine women completed the study without any...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amniotic fluid index; Maternal oral hydration; Isotonic solution.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300015

Effect of lncRNA HULC knockdown on rat secreting pituitary adenoma GH3 cells
Provedor de dados: 56
Autores: Rui,Qiu Hong; Ma,Jian Bo; Liao,Yu Feng; Dai,Jin Hua; Cai,Zhen Yu.
Pituitary adenoma is one of the most common tumors in the neuroendocrine system. This study investigated the effects of long non-coding RNAs (lncRNAs) highly up-regulated in liver cancer (HULC) on rat secreting pituitary adenoma GH3 cell viability, migration, invasion, apoptosis, and hormone secretion, as well as the underlying potential mechanisms. Cell transfection and qRT-PCR were used to change and measure the expression levels of HULC, miR-130b, and FOXM1. Cell viability, migration, invasion, and apoptosis were assessed using trypan blue staining assay, MTT assay, two-chamber transwell assay, Guava Nexin assay, and western blotting. The concentrations of prolactin (PRL) and growth hormone (GH) in culture supernatant of GH3 cells were assessed using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Secreting pituitary adenoma; LncRNA highly up-regulated in liver cancer (HULC); MicroRNA-130b; Forkhead box protein M1; PI3K/AKT/mTOR signaling pathway; JAK1/STAT3 signaling pathway.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400610

Gastroduodenal resistance and neural mechanisms involved in saline flow decrease elicited by acute blood volume expansion in anesthetized rats
Provedor de dados: 56
Autores: Graça,J.R.V.; Gondim,F. de-A.A.; Cavalcante,D.I.M.; Xavier-Neto,J.; Messias,E.L.M.; Rego,M.C.V.; Marques,J.A.P.; Santos,A.A.; Rola,F.H.
We have previously demonstrated that blood volume (BV) expansion decreases saline flow through the gastroduodenal (GD) segment in anesthetized rats (Xavier-Neto J, dos Santos AA &amp;amp; Rola FH (1990) Gut, 31: 1006-1010). The present study attempts to identify the site(s) of resistance and neural mechanisms involved in this phenomenon. Male Wistar rats (N = 97, 200-300 g) were surgically manipulated to create four gut circuits: GD, gastric, pyloric and duodenal. These circuits were perfused under barostatically controlled pressure (4 cmH2O). Steady-state changes in flow were taken to reflect modifications in circuit resistances during three periods of time: normovolemic control (20 min), expansion (10-15 min), and expanded (30 min). Perfusion flow rates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood volume expansion gastroduodenal resistance duodenum pylorus !-- $MVD$:face("Symbol") --&lt; FONT FACE=Symbol&gt; A&lt; /font&gt; -blockers vagotomy.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000019

Toxic effects of mercury, lead and gadolinium on vascular reactivity
Provedor de dados: 56
Autores: Vassallo,D.V.; Simões,M.R.; Furieri,L.B.; Fioresi,M.; Fiorim,J.; Almeida,E.A.S.; Angeli,J.K.; Wiggers,G.A.; Peçanha,F.M.; Salaices,M.
Heavy metals have been used in a wide variety of human activities that have significantly increased both professional and environmental exposure. Unfortunately, disasters have highlighted the toxic effects of metals on different organs and systems. Over the last 50 years, the adverse effects of chronic lead, mercury and gadolinium exposure have been underscored. Mercury and lead induce hypertension in humans and animals, affecting endothelial function in addition to their other effects. Increased cardiovascular risk after exposure to metals has been reported, but the underlying mechanisms, mainly for short periods of time and at low concentrations, have not been well explored. The presence of other metals such as gadolinium has raised concerns about...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heavy metal toxicity; Vascular reactivity; Mercury; Lead; Gadolinium.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900016

Chronic effect of antidopaminergic drugs or estrogen on male Wistar rat lactotrophs and somatotrophs
Provedor de dados: 56
Autores: Oliveira,M.C.; Messinger,H.P.; Tannhauser,M.; Barbosa-Coutinho,L.
The aim of the present study was to evaluate the effect of antidopaminergic agents on the somatotrophs in the presence of hyperprolactinemia. Adult male Wistar rats were divided into 6 groups: a control group and five groups chronically treated (60 days) with haloperidol, fluphenazine, sulpiride, metoclopramide or estrogen. Somatotrophs and lactotrophs were identified by immunohistochemistry and the data are reported as percent of total anterior pituitary cells counted. The drugs significantly increased the percentage of lactotrophs: control (mean ± SD) 21.3 ± 4.4, haloperidol 27.8 ± 2.2, fluphenazine 34.5 ± 3.6, sulpiride 32.7 ± 3.5, metoclopramide 33.4 ± 5.5 and estrogen 42.4 ± 2.8. A significant reduction in somatotrophs was observed in animals treated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Somatotrophs; Lactotrophs; Estrogen; Antidopaminergic drugs.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200018

Lack of tolerance for the anti-dyskinetic effects of 7-nitroindazole, a neuronal nitric oxide synthase inhibitor, in rats
Provedor de dados: 56
Autores: Novaretti,N.; Padovan-Neto,F.E.; Tumas,V.; da-Silva,C.A.; Del Bel,E.A.
7-Nitroindazole (7-NI) inhibits neuronal nitric oxide synthase in vivo and reduces l-DOPA-induced dyskinesias in a rat model of parkinsonism. The aim of the present study was to determine if the anti-dyskinetic effect of 7-NI was subject to tolerance after repeated treatment and if this drug could interfere with the priming effect of l-DOPA. Adult male Wistar rats (200-250 g) with unilateral depletion of dopamine in the substantia nigra compacta were treated with l-DOPA (30 mg/kg) for 34 days. On the 1st day, 6 rats received ip saline and 6 received ip 7-NI (30 mg/kg) before l-DOPA. From the 2nd to the 26th day, all rats received l-DOPA daily and, from the 27th to the 34th day, they also received 7-NI before l-DOPA. Animals were evaluated before the drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Nitric oxide synthase; Parkinson’s disease; L-DOPA; Dyskinesia; DOPA priming; 7-Nitroindazole.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100006

Protective effects of deferasirox and N-acetyl-L-cysteine on iron overload-injured bone marrow
Provedor de dados: 56
Autores: Shen,J.C.; Zhang,Y.C.; Zhao,M.F.
Using an iron overload mouse model, we explored the protective effect of deferasirox (DFX) and N-acetyl-L-cysteine (NAC) on injured bone marrow hematopoietic stem/progenitor cells (HSPC) induced by iron overload. Mice were intraperitoneally injected with 25 mg iron dextran every 3 days for 4 weeks to establish an iron overload (Fe) model. DFX or NAC were co-administered with iron dextran in two groups of mice (Fe+DFX and Fe+NAC), and the function of HSPCs was then examined. Iron overload markedly decreased the number of murine HSPCs in bone marrow. Subsequent colony-forming cell assays showed that iron overload also decreased the colony forming capacity of HSPCs, the effect of which could be reversed by DFX and NAC. The bone marrow hematopoiesis damage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iron overload; Bone marrow; Hematopoietic stem/progenitor cells; Deferasirox; N-acetyl-L-cysteine.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200613

Brain caspase-3 and intestinal FABP responses in preterm and term rats submitted to birth asphyxia
Provedor de dados: 56
Autores: Figueira,R.L.; Gonçalves,F.L.; Simões,A.L.; Bernardino,C.A.; Lopes,L.S.; Castro e Silva,O.; Sbragia,L.
Neonatal asphyxia can cause irreversible injury of multiple organs resulting in hypoxic-ischemic encephalopathy and necrotizing enterocolitis (NEC). This injury is dependent on time, severity, and gestational age, once the preterm babies need ventilator support. Our aim was to assess the different brain and intestinal effects of ischemia and reperfusion in neonate rats after birth anoxia and mechanical ventilation. Preterm and term neonates were divided into 8 subgroups (n=12/group): 1) preterm control (PTC), 2) preterm ventilated (PTV), 3) preterm asphyxiated (PTA), 4) preterm asphyxiated and ventilated (PTAV), 5) term control (TC), 6) term ventilated (TV), 7) term asphyxiated (TA), and 8) term asphyxiated and ventilated (TAV). We measured body, brain,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neonatal asphyxia; Necrotizing enterocolitis; I-FABP; Caspase-3; Brain injury; Intestinal damage.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700703

Regenerated rat skeletal muscle after periodic contusions
Provedor de dados: 56
Autores: Minamoto,V.B.; Bunho,S.R.; Salvini,T.F.
In the present study we evaluated the morphological aspect and changes in the area and incidence of muscle fiber types of long-term regenerated rat tibialis anterior (TA) muscle previously submitted to periodic contusions. Animals received eight consecutive traumas: one trauma per week, for eight weeks, and were evaluated one (N = 8) and four (N = 9) months after the last contusion. Serial cross-sections were evaluated by toluidine blue staining, acid phosphatase and myosin ATPase reactions. The weight of injured muscles was decreased compared to the contralateral intact one (one month: 0.77 ± 0.15 vs 0.91 ± 0.09 g, P = 0.03; four months: 0.79 ± 0.14 vs 1.02 ± 0.07 g, P = 0.0007, respectively) and showed abundant presence of split fibers and fibers with...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Skeletal muscle; Periodic contusion; Long-term regeneration; Rat.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100012

Lipoprotein lipase PvuII polymorphism is associated with variations in serum lipid levels in non-diabetic pregnant women
Provedor de dados: 56
Autores: Sepetiba,R.J.C.; Andrade,J.; Hirata,R.D.C.; Hirata,M.H.; Sepetiba,C.R.G.; Nakamura,Y.; Matsumoto,L.O.; Cavalli,S.A.; Bertolami,M.C.
The aim of the present study was to determine if there is an association between the single nucleotide polymorphisms (SNPs) of the lipoprotein lipase (LPL) and apolipoprotein E (apo E) genes and the serum lipid profile in pregnancy and puerperium. Non-diabetic women of European descent in the third semester of pregnancy (N = 120) were selected. Those with diseases or other condition that could modify their lipid profile were excluded from the study (N = 32). Serum lipids were measured by routine laboratory procedures and genomic DNA was extracted by a salting out method. LPL (PvuII and HindIII) and apo E (HhaI) SNPs were detected by the polymerase chain reaction and restriction fragment length polymorphism. Categorical and continuous variables were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipoprotein lipase; LPL PvuII polymorphism; Apolipoprotein E; Single nucleotide polymorphism; Serum lipids; Non-diabetic pregnancy.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700005

Inhibition of Mayaro virus replication by prostaglandin A1 and B2 in Vero cells
Provedor de dados: 56
Autores: Ishimaru,D.; Marcicano,F.G.P.; Rebello,M.A.
The effect of prostaglandins (PGA1 and PGB2) on the replication of Mayaro virus was studied in Vero cells. PGA1 and PGB2 antiviral activity was found to be dose-dependent. However, while 10 µg/ml PGB2 inhibited virus yield by 60%, at the same dose PGA1 suppressed virus replication by more than 90%. SDS-PAGE analysis of [35S]-methionine-labelled proteins showed that PGA1 did not alter cellular protein synthesis. In infected cells, PGA1 slightly inhibited the synthesis of protein C, while drastically inhibiting the synthesis of glycoproteins E1 and E2.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Prostaglandins; Mayaro virus; Vero cells; Protein synthesis.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900003

Effects of ethanol intake on retinol concentration in the milk of lactating rats
Provedor de dados: 56
Autores: Albuquerque,K.T.; Ramalho,R.A.; Soares,A.G.; Tavares-do-Carmo,M.G.
The effect of the consumption of ethanol (5%) on retinol concentration in milk was studied in the rat on day 12 after delivery, together with the evolution of dam body weight and pup growth rate. Female Wistar rats receiving alcohol (5%) in drinking water during lactation (N = 7) were compared to normal controls fed ad libitum (N = 6). The mean maternal alcohol intake was 3.96 ± 0.23 g/kg body weight per day. To determine retinol levels in milk we used the Bessey and Lowry method, modified by Araújo and Flores ((1978) Clinical Chemistry, 24: 386-392). The pups were separated from dams for a 2-4-h period, after which the dams were injected intraperitoneally with anesthetic and oxytocin. The concentration of retinol in milk was 162.88 ± 10.60 µg/dl in the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lactation; Ethanol; Retinol; Milk; Rat.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700007

Evaluation of a father and son with atypical chronic myeloid leukemia with SETBP1 mutations and a review of the literature
Provedor de dados: 56
Autores: Wang,L.; Du,F.; Zhang,H.-M.; Wang,H.-X.
We report the case of a father and son diagnosed with atypical chronic myeloid leukemia (aCML). Both patients harbored SETBP1 mutations, which are present in 24.3% of aCML patients. Moreover, both shared the variant encoding p.Pro737His, but the aCML severity was greater in the son because of the presence of two other missense mutations causing p.Asp868Asn and p.Ser885Arg alterations. SETBP1 mutations may be associated with an adverse prognosis, so their detection would help in the diagnosis of aCML and the determination of a patient's prognosis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atypical chronic myeloid leukemia; SETBP1 mutation; Chronic neutrophilic leukemia; CSF3R mutation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700583

Clinical and prognostic significance of serum transforming growth factor-beta1 levels in patients with pancreatic ductal adenocarcinoma
Provedor de dados: 56
Autores: Zhao,J.; Liang,Y.; Yin,Q.; Liu,S.; Wang,Q.; Tang,Y.; Cao,C.
Pancreatic ductal adenocarcinoma (PDAC) has a poor 5-year survival rate of 5%. Biomarkers for the early detection of pancreatic cancer are urgently needed. Transforming growth factor-beta1 (TGF-β1) is elevated in the tissues and plasma of patients with PDAC. However, no studies systemically report prognostic significance of plasma TGF-β1 levels in PDAC. In the present study, we assessed the prognostic significance of serum TGF-β levels in patients with PDAC. TGF-β levels were determined in serum from 146 PDAC patients, and 58 patients with benign pancreatic conditions. Regression models were used to correlate TGF-β levels to gender, age, stage, class, and metastasis. Survival analyses were performed using multivariate Cox models. Serum levels of TGF-β1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pancreatic ductal adenocarcinoma; Marker; TGF-β1.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800705

Estimation procedures affect the center of pressure frequency analysis
Provedor de dados: 56
Autores: Vieira,T.M.M.; Oliveira,L.F.; Nadal,J.
Even though frequency analysis of body sway is widely applied in clinical studies, the lack of standardized procedures concerning power spectrum estimation may provide unreliable descriptors. Stabilometric tests were applied to 35 subjects (20-51 years, 54-95 kg, 1.6-1.9 m) and the power spectral density function was estimated for the anterior-posterior center of pressure time series. The median frequency was compared between power spectra estimated according to signal partitioning, sampling rate, test duration, and detrending methods. The median frequency reliability for different test durations was assessed using the intraclass correlation coefficient. When increasing number of segments, shortening test duration or applying linear detrending, the median...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Center of pressure; Spectral analysis; Quiet standing; Estimators; Median frequency.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700012

Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor
Provedor de dados: 56
Autores: Lima,E.M.; Rissino,J.D.; Harada,M.L.; Assumpção,P.P.; Demachki,S.; Guimarães,A.C.; Casartelli,C.; Smith,M.A.C.; Burbano,R.R.
Gastric cancer is the second most frequent type of neoplasia and also the second most important cause of death in the world. Virtually all the established cell lines of gastric neoplasia were developed in Asian countries, and western countries have contributed very little to this area. In the present study we describe the establishment of the cell line ACP01 and characterize it cytogenetically by means of in vitro immortalization. Cells were transformed from an intestinal-type gastric adenocarcinoma (T4N2M0) originating from a 48-year-old male patient. This is the first gastric adenocarcinoma cell line established in Brazil. The most powerful application of the cell line ACP01 is in the assessment of cytotoxicity. Solid tumor cell lines from different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell line ACP01; Human gastric adenocarcinoma; In vitro immortalization; Chromosome 8 trisomy; Clonal expansion.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200008

Variability in baseline laboratory measurements of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil)
Provedor de dados: 56
Autores: Ladwig,R.; Vigo,A.; Fedeli,L.M.G.; Chambless,L.E.; Bensenor,I.; Schmidt,M.I.; Vidigal,P.G.; Castilhos,C.D.; Duncan,B.B.
Multi-center epidemiological studies must ascertain that their measurements are accurate and reliable. For laboratory measurements, reliability can be assessed through investigation of reproducibility of measurements in the same individual. In this paper, we present results from the quality control analysis of the baseline laboratory measurements from the ELSA-Brasil study. The study enrolled 15,105 civil servants at 6 research centers in 3 regions of Brazil between 2008–2010, with multiple biochemical analytes being measured at a central laboratory. Quality control was ascertained through standard laboratory evaluation of intra- and inter-assay variability and test-retest analysis in a subset of randomly chosen participants. An additional sample of urine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multi-center studies; Laboratory measurements; Quality control.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900701

c-Myc protein expression is not an independent prognostic predictor in cervical squamous cell carcinoma
Provedor de dados: 56
Autores: Brenna,S.M.F.; Zeferino,L.C.; Pinto,G.A.; Souza,R.A.; Andrade,L.A.L.; Vassalo,J.; Martinez,E.Z.; Syrjänen,K.J.
The c-myc protein is known to regulate the cell cycle, and its down-regulation can lead to cell death by apoptosis. The role of c-myc protein as an independent prognostic determinant in cervical cancer is controversial. In the present study, a cohort of 220 Brazilian women (mean age 53.4 years) with FIGO stage I, II and III (21, 28 and 51%, respectively) cervical squamous cell carcinomas was analyzed for c-myc protein expression using immunohistochemistry. The disease-free survival and relapse-rate were analyzed using univariate (Kaplan-Meier) survival analysis for 116 women who completed the standard FIGO treatment and were followed up for 5 years. Positive c-myc staining was detected in 40% of carcinomas, 29% being grade 1, 9% grade 2, and 2% grade 3....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-myc; Oncogene; Cervical cancer; Prognosis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400003

ß-Spectrin São PauloII, a novel frameshift mutation of the ß-spectrin gene associated with hereditary spherocytosis and instability of the mutant mRNA
Provedor de dados: 56
Autores: Bassères,D.S.; Tavares,A.C.; Costa,F.F.; Saad,S.T.O.
Hereditary spherocytosis (HS) is a common inherited anemia characterized by the presence of spherocytic red cells. Defects in several membrane protein genes have been involved in the pathogenesis of HS. ß-Spectrin-related HS seems to be common. We report here a new mutation in the ß-spectrin gene coding region in a patient with hereditary spherocytosis. The patient presented acanthocytosis and spectrin deficiency and, at the DNA level, a novel frameshift mutation leading to HS, i.e., a C deletion at codon 1392 (ß-spectrin São PauloII), exon 20. The mRNA encoding ß-spectrin São PauloII was very unstable and the mutant protein was not detected in the membrane or in other cellular compartments. It is interesting to note that frameshift mutations of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: SS-Spectrin; Red cell membrane; Spherocytosis; Acanthocytes; Mutation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800009

CaMKIIdelta overexpression in hypertrophy and heart failure: cellular consequences for excitation-contraction coupling
Provedor de dados: 56
Autores: Maier,L.S.
Ca/calmodulin-dependent protein kinase IIdelta (CaMKIIdelta) is the predominant isoform in the heart. During excitation-contraction coupling (ECC) CaMKII phosphorylates several Ca-handling proteins including ryanodine receptors (RyR), phospholamban, and L-type Ca channels. CaMKII expression and activity have been shown to correlate positively with impaired ejection fraction in the myocardium of patients with heart failure and CaMKII has been proposed to be a possible compensatory mechanism to keep hearts from complete failure. However, in addition to these acute effects on ECC, CaMKII was shown to be involved in hypertrophic signaling, termed excitation-transcription coupling (ETC). Thus, animal models have shown that overexpression of nuclear isoform...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium; Calmodulin; CaM kinase; Excitation-contraction coupling; Heart.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900002

Infrared coagulation versus rubber band ligation in early stage hemorrhoids
Provedor de dados: 56
Autores: Gupta,P.J.
The ideal therapy for early stages of hemorrhoids is always debated. Some are more effective but are more painful, others are less painful but their efficacy is also lower. Thus, comfort or efficacy is a major concern. In the present randomized study, a comparison is made between infrared coagulation and rubber band ligation in terms of effectiveness and discomfort. One hundred patients with second degree bleeding piles were randomized prospectively to either rubber band ligation (N = 54) or infrared coagulation (N = 46). Parameters measured included postoperative discomfort and pain, time to return to work, relief in incidence of bleeding, and recurrence rate. The mean age was 38 years (range 19-68 years). The mean duration of disease was 17.5 months...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemorrhoids; Infrared coagulation; Pain; Rubber band ligation; Piles.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000022

Rapid detection of multidrug-resistant Mycobacterium tuberculosis using the mycobacteria growth indicator tube (MGIT) system
Provedor de dados: 56
Autores: Telles,M.A.S.; Bori,A.; Amorim,A.B.R.; Cruz,A.F.; Pini,M.I.T.; Sato,D.N.
The emergence of multidrug-resistant strains of Mycobacterium tuberculosis has increased the need for rapid drug susceptibility tests, which are needed for adequate patient treatment. The objective of the present study was to evaluate the mycobacteria growth indicator tube (MGIT) system to detect multidrug-resistant M. tuberculosis strains. The MGIT system was compared with two standard methods (proportion and resistance ratio methods). One hundred clinical M. tuberculosis isolates [25 susceptible to isoniazid (INH) and rifampicin (RIF), 20 resistant to INH, 30 resistant to INH-RIF, and 25 resistant to INH-RIF and other drugs] obtained in the State of São Paulo were tested for INH and RIF susceptibility. Full agreement among the tests was found for all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multidrug-resistant Mycobacterium tuberculosis; Susceptibility tests; Mycobacteria growth indicator tube; Proportion method; Resistance ratio method; Multidrug resistance.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000003

VEGF receptor expression decreases during lung development in congenital diaphragmatic hernia induced by nitrofen
Provedor de dados: 56
Autores: Sbragia,L.; Nassr,A.C.C.; Gonçalves,F.L.L.; Schmidt,A.F.; Zuliani,C.C.; Garcia,P.V.; Gallindo,R.M.; Pereira,L.A.V.
Changes in vascular endothelial growth factor (VEGF) in pulmonary vessels have been described in congenital diaphragmatic hernia (CDH) and may contribute to the development of pulmonary hypoplasia and hypertension; however, how the expression of VEGF receptors changes during fetal lung development in CDH is not understood. The aim of this study was to compare morphological evolution with expression of VEGF receptors, VEGFR1 (Flt-1) and VEGFR2 (Flk-1), in pseudoglandular, canalicular, and saccular stages of lung development in normal rat fetuses and in fetuses with CDH. Pregnant rats were divided into four groups (n=20 fetuses each) of four different gestational days (GD) 18.5, 19.5, 20.5, 21.5: external control (EC), exposed to olive oil (OO), exposed to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Congenital diaphragmatic hernia; VEGFR; Rat model; Nitrofen; Lung development.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200171

Women with primary ovarian insufficiency have lower bone mineral density
Provedor de dados: 56
Autores: Amarante,F.; Vilodre,L.C.; Maturana,M.A.; Spritzer,P.M.
The aim of the present study was to assess the prevalence of osteoporosis in a sample of 32 patients with spontaneous primary ovarian insufficiency (POI) in comparison to reference groups of 25 pre- and 55 postmenopausal women. Hip (lumbar) and spinal bone mineral density (BMD) measurements were performed by dual-energy X-ray absorptiometry in the three groups. The median age of POI patients at the time of diagnosis was 35 years (interquartile range: 27-37 years). The mean ± SD age of postmenopausal reference women (52.16 ± 3.65 years) was higher than that of POI (46.28 ± 10.38 years) and premenopausal women (43.96 ± 7.08; P = 0.001) at the time of BMD measurement. Twenty-seven (84.4%) POI women were receiving hormone replacement therapy (HRT) at the time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Premature ovarian failure; Premature menopause; Osteoporosis; Bone mineral density; Hypogonadism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100012

Renal effects of uroguanylin and guanylin in vivo
Provedor de dados: 56
Autores: Carrithers,S.L.; Hill,M.J.; Johnson,B.R.; O'Hara,S.M.; Jackson,B.A.; Ott,C.E.; Lorenz,J.; Mann,E.A.; Giannella,R.A.; Forte,L.R.; Greenberg,R.N.
Uroguanylin and guanylin are newly discovered endogenous heat-stable peptides that bind to and activate a membrane bound guanylyl cyclase signaling receptor (termed guanylyl cyclase C; GC-C). These peptides are not only found in blood but are secreted into the lumen of the intestine and effect a net secretion of electrolytes (Na+, K+, Cl-, HCO3-) and fluid into the intestine via a cyclic guanosine-3',5'-monophosphate (cGMP) mechanism. GC-C is also the receptor for Escherichia coli heat-stable enterotoxin (STa) and activation by STa results in a diarrheal illness. Employing mouse renal in vivo models, we have demonstrated that uroguanylin, guanylin, and STa elicit natriuretic, kaliuretic, and diuretic effects. These biological responses are time- and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Guanylyl cyclase C; Heat-stable enterotoxin; Kidney; Natriuresis; Sodium-potassium ATPase; Differential display PCR.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100003

Physical exercise-induced fatigue: the role of serotonergic and dopaminergic systems
Provedor de dados: 56
Autores: Cordeiro,L.M.S.; Rabelo,P.C.R.; Moraes,M.M.; Teixeira-Coelho,F.; Coimbra,C.C.; Wanner,S.P.; Soares,D.D.
Brain serotonin and dopamine are neurotransmitters related to fatigue, a feeling that leads to reduced intensity or interruption of physical exercises, thereby regulating performance. The present review aims to present advances on the understanding of fatigue, which has recently been proposed as a defense mechanism instead of a “physiological failure” in the context of prolonged (aerobic) exercises. We also present recent advances on the association between serotonin, dopamine and fatigue. Experiments with rodents, which allow direct manipulation of brain serotonin and dopamine during exercise, clearly indicate that increased serotoninergic activity reduces performance, while increased dopaminergic activity is associated with increased performance....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain; Lethargy; Monoamines; Performance; Physical activity; Reward.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200301

Oxidative stress in the latissimus dorsi muscle of diabetic rats
Provedor de dados: 56
Autores: De Angelis,K.L.D.; Cestari,I.A.; Barp,J.; Dall'Ago,P.; Fernandes,T.G.; Homem de Bittencourt,P.I.; Belló-Klein,A.; Belló,A.A.; Llesuy,S.; Irigoyen,M.C.
The purpose of the present study was to investigate the effects of experimental diabetes on the oxidant and antioxidant status of latissimus dorsi (LD) muscles of male Wistar rats (220 ± 5 g, N = 11). Short-term (5 days) diabetes was induced by a single injection of streptozotocin (STZ, 50 mg/kg, iv; glycemia &gt;300 mg/dl). LD muscle of STZ-diabetic rats presented higher levels of thiobarbituric acid reactive substances (TBARS) and chemiluminescence (0.36 ± 0.02 nmol/mg protein and 14706 ± 1581 cps/mg protein) than LD muscle of normal rats (0.23 ± 0.04 nmol/mg protein and 7389 ± 1355 cps/mg protein). Diabetes induced a 92% increase in catalase and a 27% increase in glutathione S-transferase activities in LD muscle. Glutathione peroxidase activity was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Streptozotocin-diabetes; Latissimus dorsi muscle; Oxidative stress; Catalase; Glutathione.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100016

Influence of lactation on motor activity and elevated plus maze behavior
Provedor de dados: 56
Lactating rats show less noise-induced freezing and fewer inhibitory responses on the 6th day post-delivery when submitted to water and food deprivation in a classical conflict paradigm. Lactating mice go more often to the illuminated chamber in a light-dark cage and stay longer in it than virgin females. The present study was designed to assess the influence of this physiological state, i.e. lactation, on the elevated plus maze (EPM) and open-field behavior in adult female rats. Total (TL) and central (CL) locomotion and rearing (RF) frequencies were measured in an open-field. Number of entries into the open and closed arms as well as the time spent in each of these arms were measured in the EPM. Percent time spent and number of entries into the open arms...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Female rats; Anxiety; Elevated plus maze; Open-field test; Lactation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200013

Photodynamic DNA damage induced by phycocyanin and its repair in Saccharomyces cerevisiae
Provedor de dados: 56
Autores: Pádula,M.; Boiteux,S.
In the present study, we analyzed DNA damage induced by phycocyanin (PHY) in the presence of visible light (VL) using a set of repair endonucleases purified from Escherichia coli. We demonstrated that the profile of DNA damage induced by PHY is clearly different from that induced by molecules that exert deleterious effects on DNA involving solely singlet oxygen as reactive species. Most of PHY-induced lesions are single strand breaks and, to a lesser extent, base oxidized sites, which are recognized by Nth, Nfo and Fpg enzymes. High pressure liquid chromatography coupled to electrochemical detection revealed that PHY photosensitization did not induce 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) at detectable levels. DNA repair after PHY...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phycocyanin; Saccharomyces cerevisiae; OGG1; Nucleotide excision repair.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900002

Cultural adaptation and validation of the "Kidney Disease and Quality of Life - Short Form (KDQOL-SF™ 1.3)" in Brazil
Provedor de dados: 56
Autores: Duarte,P.S.; Ciconelli,R.M.; Sesso,R.
The objective of the present study was to translate the Kidney Disease Quality of Life - Short Form (KDQOL-SF™1.3) questionnaire into Portuguese to adapt it culturally and validate it for the Brazilian population. The KDQOL-SF was translated into Portuguese and back-translated twice into English. Patient difficulties in understanding the questionnaire were evaluated by a panel of experts and solved. Measurement properties such as reliability and validity were determined by applying the questionnaire to 94 end-stage renal disease patients on chronic dialysis. The Nottingham Health Profile Questionnaire, the Karnofsky Performance Scale and the Kidney Disease Questionnaire were administered to test validity. Some activities included in the original instrument...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney dialysis; Portuguese version of KDQOL-SF; End-stage renal disease; Health-related quality of life; KDQOL-SF; Test validation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200015

Glycosuria in glomerular diseases: histopathology and clinical correlations
Provedor de dados: 56
Autores: Woronik,V.; Freitas,I.F.; Saldanha,L.B.; Sabbaga,E.; Marcondes,M.
There are doubts about the presence of glycosuria and the progress of glomerular disease. Some reports suggest that glycosuria could be an index of a more severe tubulointerstitial lesion. We investigated the presence of glycosuria in 60 patients with primary glomerular diseases: 17 patients (28%) had glycosuria and 43 patients (72%) were glycosuria free. The two groups were similar in age, arterial pressure and sex. Serum creatinine was higher in patients with glycosuria (2.0 ± 1.7 vs 1.3 ± 0.9 mg/dl, P&amp;lt;0.05). The protein excretion rate was 7.5 ± 3.7 vs 5.3 ± 4.2 g/day (P&amp;gt;0.05) in patients with and without glycosuria, respectively, while serum albumin was lower in patients with glycosuria (1.7 ± 0.6 vs 2.7 ± 1.0 g/dl, P&amp;lt;0.05). Several...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glomerulonephritis; Glycosuria; Histopathology.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500005

The role of secondary hyperparathyroidism in left ventricular hypertrophy of patients under chronic hemodialysis
Provedor de dados: 56
Autores: Randon,R.B.; Rohde,L.E.; Comerlato,L.; Ribeiro,J.P.; Manfro,R.C.
End-stage renal disease (ESRD) patients frequently develop structural cardiac abnormalities, particularly left ventricular hypertrophy (LVH). The mechanisms involved in these processes are not completely understood. In the present study, we evaluated a possible association between parathyroid hormone (PTH) levels and left ventricular mass (LVM) in patients with ESRD. Stable uremic patients on intermittent hemodialysis treatment were evaluated by standard two-dimensional echocardiography and their sera were analyzed for intact PTH. Forty-one patients (mean age 45 years, range 18 to 61 years), 61% males, who had been on hemodialysis for 3 to 186 months, were evaluated. Patients were stratified into 3 groups according to serum PTH: low levels (&lt;100 pg/ml;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: End-stage renal failure; Parathyroid hormone; Echocardiography; Left ventricular hypertrophy.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900016

Effect of bacterial lipopolysaccharide on gastric emptying of liquids in rats
Provedor de dados: 56
The objectives of the present investigation were 1) to study the effect of bacterial lipopolysaccharide (LPS) on rat gastric emptying (GE) and 2) to investigate a possible involvement of the vagus nerve in the gastric action of LPS. Endotoxin from E. coli (strain 055:B5) was administered sc, ip or iv to male Wistar rats (220-280 g body weight) at a maximum dose of 50 µg/kg animal weight. Control animals received an equivalent volume of sterile saline solution. At a given time period after LPS administration, GE was evaluated by measuring gastric retention 10 min after the orogastric infusion of a test meal (2 ml/100 g animal weight), which consisted of 0.9% NaCl plus the marker phenol red (6 mg/dl). One group of animals was subjected to bilateral...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gastric emptying; Lipopolysaccharide; Vagus nerve.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200008

Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas
Provedor de dados: 56
Autores: Corrêa Junior,J.D.; Allodi,S.; Amado-Filho,G.M.; Farina,M.
Amorphous phosphate granules are present in vertebrate and invertebrate organisms. The functions attributed to these structures depend on their mineral contents and organic matrix composition. In the present study we have determined zinc concentrations in the hepatopancreas of the crab Ucides cordatus from regions contaminated with zinc, and the elemental composition of hepatopancreal phosphate granules. Organisms were collected from the contaminated areas of Sepetiba Bay (SB) and Guanabara Bay (GB), and from a non-contaminated area, Ribeira Bay (RB). The first two sites are located near the metropolitan region of Rio de Janeiro city, Brazil. Atomic absorption spectroscopy (AAS) showed a significant difference (P&amp;lt;0.05) for zinc concentration in the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Phosphate granules; Hepatopancreas; Zinc; Energy dispersive X-ray analysis; Atomic absorption spectroscopy; Electron spectroscopic imaging.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200009

Effect of electrolytic lesion of the dorsal raphe nucleus on water intake and sodium appetite
Provedor de dados: 56
Autores: Olivares,E.L.; Costa-e-Sousa,R.H.; Cavalcante-Lima,H.R.; Lima,H.R.C.; Cedraz-Mercez,P.L.; Reis,L.C.
The present study determined the effect of an electrolytic lesion of the dorsal raphe nucleus (DRN) on water intake and sodium appetite. Male Wistar rats weighing 290-320 g with a lesion of the DRN (L-DRN), performed two days before experiments and confirmed by histology at the end of the experiments, presented increased sensitivity to the dehydration induced by fluid deprivation. The cumulative water intake of L-DRN rats reached 23.3 ± 1.9 ml (a 79% increase, N = 9) while sham-lesioned rats (SL-DRN) did not exceed 13.0 ± 1.0 ml (N = 11, P &lt; 0.0001) after 5 h. The L-DRN rats treated with isoproterenol (300 µg kg-1 ml-1, sc) exhibited an increase in water intake that persisted throughout the experimental period (L-DRN, 15.7 ± 1.47 ml, N = 9 vs SL-DRN,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sodium appetite; Water intake; Dorsal raphe nucleus; Serotonergic system; Electrolytic lesion; Rats.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200013

Trends in drug use among students in Brazil: analysis of four surveys in 1987, 1989, 1993 and 1997
Provedor de dados: 56
Autores: Galduróz,J.C.F.; Noto,A.R.; Nappo,S.A.; Carlini,E.A.
The consumption of psychotropic drugs among Brazilian secondary school students was examined by comparing data from four surveys using a questionnaire adapted from the WHO's Program on Research and Reporting on the Epidemiology of Drug Dependence. Students filled out the form in their classrooms without the presence of teachers. The target population consisted of 10-18-year-old students (on average, 15,000 students responded to each survey) in Brazil's ten largest state capitals: Belém, Belo Horizonte, Brasília, Curitiba, Fortaleza, Porto Alegre, Recife, Rio de Janeiro, Salvador, and São Paulo. Among the legal drugs, lifetime use (use at least once during life) of tobacco was increased in seven cities (the exceptions were Brasília, Porto Alegre and Rio de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Students; Trends; Inhalants; Cocaine; Marijuana; Surveys; Brazilian students.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400009

Plasmablastic multiple myeloma is associated with increased vascular endothelial growth factor immunoexpression
Provedor de dados: 56
Autores: Ribas,C.; Colleoni,G.W.B.; Almeida,M.S.S.; Aguiar,K.C.C.; Silva,M.R.R.; Bordin,J.O.
The biologic basis of the negative prognosis of plasmablastic myeloma is not fully understood. To determine whether histologically aggressive multiple myeloma (MM) is associated with a more angiogenic marrow environment, bone marrow samples from 50 recently diagnosed MM patients were evaluated. Twelve percent (6/50) of patients presented plasmablastic MM, and this feature correlated with moderate/strong intensity of vascular endothelial growth factor staining of plasma cells (P = 0.036). Although plasmablastic MM was not associated with increasing of microvessel density, this new evidence of increased expression of vascular endothelial growth factor on plasmablasts suggests that the adverse prognosis conferred by plasmablastic disease may be due, at least...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiple myeloma; Angiogenesis; Vascular endothelial growth factor.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100007

The role of natural killer cells in the early period of infection in murine cutaneous leishmaniasis
Provedor de dados: 56
Autores: Laurenti,M.D.; Gidlund,M.; Ura,D.M.; Sinhorini,I.L.; Corbett,C.E.P.; Goto,H.
In order to study the role of natural killer (NK) cells during the early period of Leishmania infection, BALB/c mice were selectively and permanently depleted of NK cells by injection with 90Sr and subsequently infected with Leishmania (Leishmania) amazonensis (HSJD-1 strain). 90Sr is known to selectively deplete NK cells, leaving an intact T- and B-cell compartment and preserving the ability to produce both interferon alpha and IL-2. This method of depletion has advantages when compared with depletion using anti-NK cell monoclonal antibodies because the effect is permanent and neither activates complement nor provokes massive cell death. In the present study, after one month of treatment with 90Sr, the depletion of NK cells was shown by a more than...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cutaneous leishmaniasis; NK cells; Strontium 90; Leishmania (Leishmania) amazonensis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300012

Molecular identification of Sicilian (dß)º-thalassemia associated with ß-thalassemia and hemoglobin S in Brazil
Provedor de dados: 56
Autores: Andrade,T.G. de; Fattori,A.; Saad,S.T.O.; Sonati,M.F.; Costa,F.F.
We describe the clinical and molecular characteristics of two unrelated Brazilian families with an association of the Sicilian form of (dß)º-thalassemia with hemoglobin S and ß-thalassemia. Direct sequencing of the ß-globin gene showed only the hemoglobin S mutation in patient 1 and the ß-thalassemia IVS1-110 in patient 2. The other allele was deleted in both patients and PCR of DNA samples of the breakpoint region of both patients showed a band of approximately 1,150 bp, expected to be observed in the DNA of carriers of Sicilian (dß)º-thalassemia. The nucleotide sequence of this fragment confirmed the Sicilian deletion. There are few reports concerning the Hb S/(dß)º-thalassemia association and patient 2 is the first reported case of Sicilian type of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Thalassemia; Sickle cell; Hereditary persistence of fetal hemoglobin; Globin genes; Sicilian thalassemia.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800003

Chronic experimental myocardial infarction produces antinatriuresis by a renal nerve-dependent mechanism
Provedor de dados: 56
Autores: Souza,D.R.B.; Mill,J.G.; Cabral,A.M.
The present study focused on the role of sympathetic renal nerve activity, in mediating congestive heart failure-induced sodium retention following experimental chronic myocardial infarction. Groups of male Wistar rats (240-260 g) were studied: sham-operated coronary ligation (CON3W, N = 11), coronary ligation and sham-operated renal denervation (INF3W, N = 19), 3 weeks of coronary ligation and sympathetic renal nerve denervation (INF3WDX, N = 6), sham-operated coronary ligation (N = 7), and 16 weeks of coronary ligation (INF16W, N = 7). An acute experimental protocol was used in which the volume overload (VO; 5% of body weight) was applied for 30 min after the equilibration period of continuous iv infusion of saline. Compared to control levels, VO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal denervation; Heart failure; Renal sympathetic nerves; Renal excretory function; Urinary sodium excretion.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200017

Hyperhomocystinemia in patients with coronary artery disease
Provedor de dados: 56
Autores: Faria-Neto,J.R.; Chagas,A.C.P.; Bydlowski,S.P.; Lemos Neto,P.A.; Chamone,D.A.; Ramirez,J.A.F.; da Luz,P.L.
Hyperhomocystinemia has been related to an increased risk of cardiovascular disease in several studies. The C677T polymorphism for the gene that encodes the methylenetetrahydrofolate reductase enzyme (MTHFR) and low plasma folate levels are common causes of hyperhomocystinemia. Due to differences in nutritional patterns and genetic background among different countries, we evaluated the role of hyperhomocystinemia as a coronary artery disease (CAD) risk factor in a Brazilian population. The relation between homocysteine (Hcy) and the extent of CAD, measured by an angiographic score, was determined. A total of 236 patients referred for coronary angiography for clinical reasons were included. CAD was found in 148 (62.7%) patients and 88 subjects had normal or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperhomocystinemia; Homocysteine; Methylenetetrahydrofolate reductase; Atherosclerosis; Folic acid deficiency.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400005

Increase in skeletal muscle protein content by the ß-2 selective adrenergic agonist clenbuterol exacerbates hypoalbuminemia in rats fed a low-protein diet
Provedor de dados: 56
Autores: Sawaya,A.L.; Lunn,P.G.
This investigation examined how the nutritional status of rats fed a low-protein diet was affected when the animals were treated with the ß-2 selective agonist clenbuterol (CL). Males (4 weeks old) from an inbred, specific-pathogen-free strain of hooded rats maintained at the Dunn Nutritional Laboratory were used in the experiments (N = 6 rats per group). CL treatment (Ventipulmin, Boehringer-Ingelheim Ltd., 3.2 mg/kg diet for 2 weeks) caused an exacerbation of the symptoms associated with protein deficiency in rats. Plasma albumin concentrations, already low in rats fed a low-protein diet (group A), were further reduced in CL rats (A = 25.05 ± 0.31 vs CL = 23.64 ± 0.30 g/l, P&amp;lt;0.05). Total liver protein decreased below the level seen in either...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein-energy malnutrition; Rats; Clenbuterol; Hypoalbuminemia; Brown adipose tissue; SS-2 agonist.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600015

Nitric oxide, cholesterol oxides and endothelium-dependent vasodilation in plasma of patients with essential hypertension
Provedor de dados: 56
Autores: Moriel,P.; Sevanian,A.; Ajzen,S.; Zanella,M.T.; Plavnik,F.L.; Rubbo,H.; Abdalla,D.S.P.
The objective of the present study was to identify disturbances of nitric oxide radical (·NO) metabolism and the formation of cholesterol oxidation products in human essential hypertension. The concentrations of·NO derivatives (nitrite, nitrate, S-nitrosothiols and nitrotyrosine), water and lipid-soluble antioxidants and cholesterol oxides were measured in plasma of 11 patients with mild essential hypertension (H: 57.8 ± 9.7 years; blood pressure, 148.3 ± 24.8/90.8 ± 10.2 mmHg) and in 11 healthy subjects (N: 48.4 ± 7.0 years; blood pressure, 119.4 ± 9.4/75.0 ± 8.0 mmHg).Nitrite, nitrate and S-nitrosothiols were measured by chemiluminescence and nitrotyrosine was determined by ELISA. Antioxidants were determined by reverse-phase HPLC and cholesterol oxides...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitrotyrosine; S-nitrosothiols; Nitrate; Oxidized LDL; Lipid peroxidation; Endothelin-1.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100007

Myocardial remodeling and bioelectric changes in tachycardia-induced heart failure in dogs
Provedor de dados: 56
Autores: Song,B.; Wang,B.N.; Chen,D.N.; Luo,Z.G.
In this study, electrical and structural remodeling of ventricles was examined in tachycardia-induced heart failure (HF). We studied two groups of weight-matched adult male mongrel dogs: a sham-operated control group (n=5) and a pacing group (n=5) that underwent ventricular pacing at 230 bpm for 3 weeks. Clinical symptoms of congestive HF were observed in both groups. Their hemodynamic parameters were determined and the severity of the HF was evaluated by M-mode echocardiography. Changes in heart morphology were observed by scanning electron and light microscopy. Ventricular action potential duration (APD), as well as the 50 and 90% APD were measured in both groups. All dogs exhibited clinical symptoms of congestive HF after rapid right ventricular pacing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tachycardia; Heart failure; Action potential duration.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900797

Catecholamine response to exercise in individuals with different levels of paraplegia
Provedor de dados: 56
Autores: Steinberg,L.L.; Lauro,F.A.A.; Sposito,M.M.M.; Tufik,S.; Mello,M.T.; Naffah-Mazzacoratti,M.G.; Cavalheiro,E.A.; Silva,A.C.
The purpose of this study was to investigate the effect of the level of injury on the serum level of norepinephrine (Nor) and epinephrine (Epi) at rest and after maximal exercise in individuals with paraplegia. Twenty-six male spinal cord-injured subjects with complete paraplegia for at least 9 months were divided into two groups of 13 subjects each according to the level of injury, i.e., T1-T6 and T7-T12. Serum Nor and Epi concentrations were measured by HPLC-ECD, at rest (PRE) and immediately after a maximal ergospirometric test (POST). Statistical analysis was performed using parametric and non-parametric tests. Maximal heart rate, peak oxygen uptake, and PRE and POST Nor were lower in the T1-T6 than in the T7-T12 group (166 ± 28 vs 188 ± 10 bpm; 18.0 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catecholamines; Paraplegia; Exercise; Norepinephrine; Epinephrine; Spinal cord injury.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800007

Effect of dietary linoleic acid on the progression of chronic renal failure in rats
Provedor de dados: 56
Autores: Gregório,S.M.P.; Lemos,C.C.S.; Caldas,M.L.; Bregman,R.
The role of linoleic acid in chronic renal failure (CRF) is controversial. In the present study 21 male Wistar rats submitted to 5/6 renal mass reduction (R) and 16 normal controls (C) were fed a supplement (S) or normal (N) linoleic acid diet for 60 days starting 10 days after CRF. As expected, serum creatinine, cholesterol and triglycerides (mean ± SEM) were higher in the CRF groups compared to the C groups (P&lt;0.05). The RS group presented lower cholesterol (84 ± 4 vs 126 ± 13 mg%) and triglyceride (88 ± 9 vs 132 ± 19 mg%) levels compared to the RN group. Proteinuria and kidney weight did not differ between CRF groups. Glomerular area increased 78% in RS and 100% in RN compared to control rats. Glomerular sclerosis index tended to be lower in RS (27%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic kidney failure; Diet; Glomeruli; Linoleic acid.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500010

Novel donors of nitric oxide derived of S-nitrosocysteine possessing antioxidant activities
Provedor de dados: 56
Autores: Petit,C.; Bernardes-Genisson,V.; Hoffmann,P.; Souchard,J.-P.; Labidalle,S.
Novel S-nitrosothiols possessing a phenolic function were investigated as nitric oxide (NO) donors. A study of NO release from these derivatives was carried out by electron spin resonance (ESR). All compounds gave rise to a characteristic three-line ESR signal in the presence of the complex [Fe(II)(MGD)2], revealing the formation of the complex [Fe(II)(MGD)2(NO)]. Furthermore, tests based on cytochrome c reduction were performed in order to study the ability of each phenolic disulfide, the final organic decomposition product of S-nitrosothiols, to trap superoxide radical anion (O2-). This study revealed a high reactivity of 1b and 3b towards O2-. For these two compounds, the respective inhibitory concentration (IC) 50 values were 92 µM and 43 µM.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; S-nitrosothiols (thionitrites); Antioxidant; Peroxynitrite.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100011

Nutritional status and body composition after 6 months of patients switching from continuous ambulatorial peritoneal dialysis to automated peritoneal dialysis
Provedor de dados: 56
Autores: Garcia-Lopes,M.G.; Agliussi,R.G.; Avesani,C.M.; Manfredi,S.R.; Bazanelli,A.P.; Kamimura,M.A.; Draibe,S.A.; Cuppari,L.
Our objective was to determine if automated peritoneal dialysis (APD) leads to changes in nutritional parameters of patients treated by continuous ambulatory peritoneal dialysis (CAPD). Twenty-six patients (15 males; 50.5 ± 14.3 years) were evaluated during CAPD while training for APD and after 3 and 6 months of APD. Body fat was assessed by the sum of skinfold thickness and the other body compartments were assessed by bioelectrical impedance. During the 6-month follow-up, 12 patients gained more than 1 kg (GW group), 8 patients lost more than 1 kg (LW group), and 6 patients maintained body weight (MW group). Except for length on dialysis that was longer for the LW group compared with the GW group, no other differences were found between the groups at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic kidney disease; Peritoneal dialysis; Body composition; Nutritional parameters.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200012

Tyrosine phosphorylation of Kv1.5 is upregulated in intrauterine growth retardation rats with exaggerated pulmonary hypertension
Provedor de dados: 56
Autores: Fu,L.C.; Lv,Y.; Zhong,Y.; He,Q.; Liu,X.; Du,L.Z.
Intrauterine growth retardation (IUGR) is associated with the development of adult-onset diseases, including pulmonary hypertension. However, the underlying mechanism of the early nutritional insult that results in pulmonary vascular dysfunction later in life is not fully understood. Here, we investigated the role of tyrosine phosphorylation of voltage-gated potassium channel 1.5 (Kv1.5) in this prenatal event that results in exaggerated adult vascular dysfunction. A rat model of chronic hypoxia (2 weeks of hypoxia at 12 weeks old) following IUGR was used to investigate the physiological and structural effect of intrauterine malnutrition on the pulmonary artery by evaluating pulmonary artery systolic pressure and vascular diameter in male rats. Kv1.5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IUGR; Kv1.5; PASMCs; Pulmonary hypertension; Tyrosine phosphorylation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100604

Evidence for the endophytic colonization of Phaseolus vulgaris(common bean) roots by the diazotroph Herbaspirillum seropedicae
Provedor de dados: 56
Autores: Schmidt,M.A.; Souza,E.M.; Baura,V.; Wassem,R.; Yates,M.G.; Pedrosa,F.O.; Monteiro,R.A.
Herbaspirillum seropedicae is an endophytic diazotrophic bacterium, which associates with important agricultural plants. In the present study, we have investigated the attachment to and internal colonization of Phaseolus vulgaris roots by the H. seropedicae wild-type strain SMR1 and by a strain of H. seropedicae expressing a red fluorescent protein (DsRed) to track the bacterium in the plant tissues. Two-day-old P. vulgaris roots were incubated at 30°C for 15 min with 6 x 10(8) CFU/mL H. seropedicae SMR1 or RAM4. Three days after inoculation, 4 x 10(4) cells of endophytic H. seropedicae SMR1 were recovered per gram of fresh root, and 9 days after inoculation the number of endophytes increased to 4 x 10(6) CFU/g. The identity of the recovered bacteria was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbaspirillum seropedicae; Phaseolus vulgaris; Confocal microscopy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300001

Variability of Vernier acuity measurements in untrained subjects of different ages
Provedor de dados: 56
Autores: Abbud,C.M.M.; Cruz,A.A.V.
The Vernier acuity of 50 normal untrained subjects (20 males and 30 females) was measured by the method of adjustment. Subjects were divided into five age ranges with 10 subjects in each age group: 5-10, 11-20, 21-30, 31-40, and 41-50 years. All subjects had normal visual acuity (20/20) and volunteered to participate in the experiment. Children were selected from a local school and adults recruited from the technical staff of the Department of Ophthalmology of the School of Medicine. Vernier acuity was higher in adults compared to children. Intraindividual variability was high and it was estimated that for most individuals of all age groups a range of 100 to 700 trials was necessary to obtain a mean with a precision of 10%. These results suggest that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vernier acuity; Variability; Age.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200011

Short-term and long-term antibody response by mice after immunization against Neisseria meningitidis B or diphtheria toxoid
Provedor de dados: 56
Autores: Silva,G.P.; Cruz,S.C.; Cruz,A.C.; Milagres,L.G.
Serogroup B Neisseria meningitidis (MenB) is a major cause of invasive disease in early childhood worldwide. The only MenB vaccine available in Brazil was produced in Cuba and has shown unsatisfactory efficacy when used to immunize millions of children in Brazil. In the present study, we compared the specific functional antibody responses evoked by the Cuban MenB vaccine with a standard vaccine against diphtheria (DTP: diphtheria, tetanus, pertussis) after primary immunization and boosting of mice. The peak of bactericidal and opsonic antibody titers to MenB and of neutralizing antibodies to diphtheria toxoid (DT) was reached after triple immunization with the MenB vaccine or DTP vaccine, respectively. However, 4 months after immunization, protective DT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serological memory; Antibody recall response; Booster immunization.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200148

Association between tumor necrosis factor polymorphisms and rheumatoid arthritis as well as systemic lupus erythematosus: a meta-analysis
Provedor de dados: 56
Autores: Chen,Lin; Huang,Zhuochun; Liao,Yun; Yang,Bin; Zhang,Junlong.
Tumor necrosis factor-alpha (TNF-α) plays an important role in autoimmune diseases. Previous studies have investigated the association of TNF-α-238G/A (rs361525) and -308G/A (rs1800629) polymorphisms with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). However, no agreed conclusion had been made. Therefore, this meta-analysis was conducted to assess the associations of TNF-α-238G/A and -308G/A polymorphisms with RA and SLE risk. A systematic search was conducted in commonly used databases. Meta-analysis was performed by STATA12.0. A total of 43 studies were included. In the overall population, the TNF-α-238A allele was observed to be a protective factor for RA (A vs G: OR=0.75, 95%CI=0.57–0.99, P=0.040) and the TNF-α-308A allele was found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheumatoid arthritis; Single-nucleotide polymorphism; Systemic lupus erythematosus; Tumor necrosis factor-alpha; Meta-analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300607

The brain decade in debate: II. Panic or anxiety? From animal models to a neurobiological basis
Provedor de dados: 56
Autores: Andreatini,R.; Blanchard,C.; Blanchard,R.; Brandão,M.L.; Carobrez,A.P.; Griebel,G.; Guimarães,F.S.; Handley,S.L.; Jenck,F.; Leite,J.R.; Rodgers,J.; Schenberg,L.C.; Da Cunha,C.; Graeff,F.G.
This article is a transcription of an electronic symposium sponsored by the Brazilian Society of Neuroscience and Behavior (SBNeC). Invited researchers from the European Union, North America and Brazil discussed two issues on anxiety, namely whether panic is a very intense anxiety or something else, and what aspects of clinical anxiety are reproduced by animal models. Concerning the first issue, most participants agreed that generalized anxiety and panic disorder are different on the basis of clinical manifestations, drug response and animal models. Also, underlying brain structures, neurotransmitter modulation and hormonal changes seem to involve important differences. It is also common knowledge that existing animal models generate different types of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Panic; Anxiety; Fear; Anxiolytics; 5-HT; Benzodiazepines; Serotonin; Amygdala; Periaqueductal gray matter; Animal models.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200001

Baicalein restrains proliferation, migration, and invasion of human malignant melanoma cells by down-regulating colon cancer associated transcript-1
Provedor de dados: 56
Autores: Yang,Xiaoliang; Jiang,Jinjie; Zhang,Chunyan; Li,Yinghao.
Baicalein (BAI) is an acknowledged flavonoids compound, which is regarded as a useful therapeutic pharmaceutical for numerous cancers. However, its involvement in melanoma is largely unknown. This study aimed to examine the anti-melanoma function of BAI and unraveled the regulatory mechanism involved. A375 and SK-MEL-28 were treated with BAI for 24 h. Then, CCK-8 assay, flow cytometry, and transwell assay were carried out to investigate cell growth, migration, and invasion. RT-qPCR was applied to detect the expression of colon cancer associated transcript-1 (CCAT1) in melanoma tissues and cells. The functions of CCAT1 in melanoma cells were also evaluated. Western blot was utilized to appraise Wnt/β-catenin or MEK/ERK pathways. BAI restrained cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malignant melanoma; Colon cancer associated transcript-1; Wnt/β-catenin; MEK/ERK.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200602

Oral administration of L-arginine decreases blood pressure and increases renal excretion of sodium and water in renovascular hypertensive rats
Provedor de dados: 56
Autores: Gouvêa,S.A.; Moysés,M.R.; Bissoli,N.S.; Pires,J.G.P.; Cabral,A.M.; Abreu,G.R.
The two-kidney, one-clip renovascular (2K1C) hypertension model is characterized by a reduction in renal flow on the clipped artery that activates the renin-angiotensin system. Endothelium dysfunction, including diminished nitric oxide production, is also believed to play a role in the pathophysiology of this model. Some studies have shown an effect of L-arginine (L-Arg, a nitric oxide precursor) on hypertension. In the present study we determined the ability of L-Arg (7 days of treatment) to reduce blood pressure and alter renal excretions of water, Na+ and K+ in a model of 2K1C-induced hypertension. Under ether anesthesia, male Wistar rats (150-170 g) had a silver clip (0.20 mm) placed around the left renal artery to produce the 2K1C renovascular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-arginine; Nitric oxide; Renovascular hypertension; Sodium excretion; Renal physiology; Blood pressure.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700017

Identification of a protein kinase activity that phosphorylates connexin43 in a pH-dependent manner
Provedor de dados: 56
Autores: Yahuaca,P.; Ek-Vitorin,J.F.; Rush,P.; Delmar,M.; Taffet,S.M.
The carboxyl-terminal (CT) domain of connexin43 (Cx43) has been implicated in both hormonal and pH-dependent gating of the gap junction channel. An in vitro assay was utilized to determine whether the acidification of cell extracts results in the activation of a protein kinase that can phosphorylate the CT domain. A glutathione S-transferase (GST)-fusion protein was bound to Sephadex beads and used as a target for protein kinase phosphorylation. A protein extract produced from sheep heart was allowed to bind to the fusion protein-coated beads. The bound proteins were washed and then incubated with 32P-ATP. Phosphorylation was assessed after the proteins were resolved by SDS-PAGE. Incubation at pH 7.5 resulted in a minimal amount of phosphorylation while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Connexin; Phosphorylation; Phosphotransferases; Protein kinase.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400005

alpha-Smooth muscle actin and proliferating cell nuclear antigen expression in focal segmental glomerulosclerosis: functional and structural parameters of renal disease progression
Provedor de dados: 56
Autores: Geleilete,T.J.M.; Costa,R.S.; Dantas,M.; Coimbra,T.M.
The aim of the present study was to investigate the expression of alpha-smooth muscle actin (alpha-SM-actin) and proliferating cell nuclear antigen (PCNA) in renal cortex from patients with focal segmental glomerulosclerosis (FSGS) and their correlations with parameters of renal disease progression. We analyzed renal biopsies from 41 patients with idiopathic FSGS and from 14 control individuals. The alpha-SM-actin immunoreaction was evaluated using a score that reflected the changes in the extent and intensity of staining in the glomerular or cortical area. The PCNA reaction was quantified by counting the labeled cells of the glomeruli or renal cortex. The results, reported as median ± percentile (25th; 75th), showed that the alpha-SM-actin scores in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha-smooth muscle actin; Proliferating cell nuclear antigen; PCNA; Focal segmental glomerulosclerosis; Proteinuria; Renal failure; Renal disease progression; Renal fibrosis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800003

Remodeling in the ischemic heart: the stepwise progression for heart
Provedor de dados: 56
Autores: Stefanon,I.; dos Santos,L.; Baldo,M.P.
Abstract Coronary artery disease is the leading cause of death in the developed world and in developing countries. Acute mortality from acute myocardial infarction (MI) has decreased in the last decades. However, the incidence of heart failure (HF) in patients with healed infarcted areas is increasing. Therefore, HF prevention is a major challenge to the health system in order to reduce healthcare costs and to provide a better quality of life. Animal models of ischemia and infarction have been essential in providing precise information regarding cardiac remodeling. Several of these changes are maladaptive, and they progressively lead to ventricular dilatation and predispose to the development of arrhythmias, HF and death. These events depend on cell death...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary occlusion; Inflammation; Collagen remodeling; Mechanical dysfunction; Heart failure; Rats.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900010

Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats
Provedor de dados: 56
Autores: Hermel,E.E.S.; Severino,G.S.; Cecconello,A.L.; Pereira,F.M.; Sanvitto,G.L.; Lucion,A.B.
Neonatal handling has long-lasting effects on behavior and stress reactivity. The purpose of the present study was to investigate the effect of neonatal handling on the number of dopaminergic neurons in the hypothalamic nuclei of adult male rats as part of a series of studies that could explain the long-lasting effects of neonatal stimulation. Two groups of Wistar rats were studied: nonhandled (pups were left undisturbed, control) and handled (pups were handled for 1 min once a day during the first 10 days of life). At 75-80 days, the males were anesthetized and the brains were processed for immunohistochemistry. An anti-tyrosine hydroxylase antibody and the avidin-biotin-peroxidase method were used. Tyrosine hydroxylase-immunoreactive (TH-IR) neurons were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Stress; Neonatal stimulation; Dopamine; Immunohistochemistry; Hypothalamus; Tyrosine hydroxylase.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900013

Participation of neuronal nitric oxide synthase in experimental neuropathic pain induced by sciatic nerve transection
Provedor de dados: 56
Autores: Chacur,M.; Matos,R.J.B.; Alves,A.S.; Rodrigues,A.C.; Gutierrez,V.; Cury,Y.; Britto,L.R.G.
Nerve injury leads to a neuropathic pain state that results from central sensitization. This phenomenom is mediated by NMDA receptors and may involve the production of nitric oxide (NO). In this study, we investigated the expression of the neuronal isoform of NO synthase (nNOS) in the spinal cord of 3-month-old male, Wistar rats after sciatic nerve transection (SNT). Our attention was focused on the dorsal part of L3-L5 segments receiving sensory inputs from the sciatic nerve. SNT resulted in the development of neuropathic pain symptoms confirmed by evaluating mechanical hyperalgesia (Randall and Selitto test) and allodynia (von Frey hair test). Control animals did not present any alteration (sham-animals). The selective inhibitor of nNOS, 7-nitroindazole...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allodynia; Neuropathic pain; Neuronal nitric oxide synthase; Peripheral neuropathy; Sciatic nerve transection; Spinal cord.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400008

A study of the interaction between Helicobacter pylori and components of the human fibrinolytic system
Provedor de dados: 56
Autores: Yarzábal,A.; Avilán,L.; Hoelzl,K.; Muñoz,M. de; Puig,J.; Kansau,I.
The interaction of plasminogen, tissue plasminogen activator (t-PA) and urokinase with a clinical strain of Helicobacter pylori was studied. Plasminogen bound to the surface of H. pylori cells in a concentration-dependent manner and could be activated to the enzymatic form, plasmin, by t-PA. Affinity chromatography assays revealed a plasminogen-binding protein of 58.9 kDa in water extracts of surface proteins. Surface-associated plasmin activity, detected with the chromogenic substrate CBS 00.65, was observed only when plasminogen and an exogenous activator were added to the cell suspension. The two physiologic plasminogen activators, t-PA and urokinase, were also shown to bind to and remain active on the surface of bacterial cells. epsilon-Aminocaproic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Plasminogen; T-PA; Urokinase; Plasminogen activation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900004

Control of gene expression in Trypanosomatidae
Provedor de dados: 56
Autores: Teixeira,S.M.R.
The study of mechanisms which control gene expression in trypanosomatids has developed at an increasing rate since 1989 when the first successful DNA transfection experiments were reported. Using primarily Trypanosoma brucei as a model, several groups have begun to elucidate the basic control mechanisms and to define the cellular factors involved in mRNA transcription, processing and translation in these parasites. This review focuses on the most recent studies regarding a subset of genes that are expressed differentially during the life cycle of three groups of parasites. In addition to T. brucei, I will address studies on gene regulation in a few species of Leishmania and the results obtained by a much more limited group of laboratories studying gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma; Leishmania; Gene expression; RNA; Antigenic variation; VSG.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200001

Small-scale environmental enrichment and exercise enhance learning and spatial memory of Carassius auratus, and increase cell proliferation in the telencephalon: an exploratory study
Provedor de dados: 56
Autores: Abreu,C.C.; Fernandes,T.N.; Henrique,E.P.; Pereira,P.D.C.; Marques,S.B.; Herdeiro,S.L.S.; Oliveira,F.R.R.; Magalhães,N.G.M.; Anthony,D.C.; Melo,M.A.D.; Guerreiro-Diniz,C.; Diniz,D.G.; Picanço-Diniz,C.W.
Carassius auratus is a teleost fish that has been largely used in behavioral studies. However, little is known about potential environmental influences on its performance of learning and memory tasks. Here, we investigated this question in C. auratus, and searched for potential correlation between exercise and visuospatial enrichment with the total number of telencephalic glia and neurons. To that end, males and females were housed for 183 days in either an enriched (EE) or impoverished environment (IE) aquarium. EE contained toys, natural plants, and a 12-hour/day water stream for voluntary exercise, whereas the IE had none of the above. A third plus-maze aquarium was used for spatial and object recognition tests. Different visual clues in 2 of its 4 arms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carassius auratus; Spatial learning and memory; Object recognition; Stereology; Telencephalon; Environmental enrichment.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500602

Effects of prenatal exposure to a mild chronic variable stress on body weight, preweaning mortality and rat behavior
Provedor de dados: 56
Autores: Cabrera,R.J.; Rodríguez-Echandía,E.L.; Jatuff,A.S.G.; Fóscolo,M.
Early stimulation has been shown to produce long-lasting effects in many species. Prenatal exposure to some strong stressors may affect development of the nervous system leading to behavioral impairment in adult life. The purpose of the present work was to study the postnatal harmful effects of exposure to variable mild stresses in rats during pregnancy. Female Holtzman rats were submitted daily to one session of a chronic variable stress (CVS) during pregnancy (prenatal stress; PS group). Control pregnant rats (C group) were undisturbed. The pups of PS and C dams were weighed and separated into two groups 48 h after delivery. One group was maintained with their own dams (PS group, N = 70; C group, N = 36) while the other PS pups were cross-fostered with C...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prenatal stress; Chronic stress; Body weight; Behavior; Mortality; Rat.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000009

New multilocus sequence typing of MRSA in São Paulo, Brazil
Provedor de dados: 56
Autores: Carmo,M.S.; Inoue,F.; Andrade,S.S.; Paschoal,L.; Silva,F.M.; Oliveira,V.G.S.; Pignatari,A.C.C.
An increased incidence of nosocomial and community-acquired infections caused by methicillin-resistant Staphylococcus aureus (MRSA) has been observed worldwide. The molecular characterization of MRSA has played an important role in demonstrating the existence of internationally disseminated clones. The use of molecular biology methods in the surveillance programs has enabled the tracking of MRSA spread within and among hospitals. These data are useful to alert nosocomial infection control programs about the potential introduction of these epidemic clones in their areas. Four MRSA blood culture isolates from patients hospitalized at two hospitals in the city of São Paulo, Brazil, were analyzed; one of them was community acquired. The isolates were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methicillin-resistant Staphylococcus aureus; Molecular sequence typing; Resistance; Molecular typing.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000006

Periodic mechanical stress activates MEK1/2-ERK1/2 mitogenic signals in rat chondrocytes through Src and PLCγ1
Provedor de dados: 56
Autores: Ren,Kewei; Ma,Yimin; Huang,Yumin; Liang,Wenwei; Liu,Feng; Wang,Qing; Cui,Weiding; Liu,Zhengyu; Yin,Guoyong; Fan,Weimin.
The mitogenic effects of periodic mechanical stress on chondrocytes have been studied extensively but the mechanisms whereby chondrocytes sense and respond to periodic mechanical stress remain a matter of debate. We explored the signal transduction pathways of chondrocyte proliferation and matrix synthesis under periodic mechanical stress. In particular, we sought to identify the role of the MEK1/2-ERK1/2 signaling pathway in chondrocyte proliferation and matrix synthesis following cyclic physiologic mechanical compression. Under periodic mechanical stress, both rat chondrocyte proliferation and matrix synthesis were significantly increased (P &lt; 0.05) and were associated with increases in the phosphorylation of Src, PLCγ1, MEK1/2, and ERK1/2 (P &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Periodic mechanical stress; Chondrocyte proliferation and matrix synthesis; Src; PLCγ1; MEK1/2-ERK1/2.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200006

In situ enzyme immunoassay for titration of a Brazilian hepatitis A virus strain (HAF-203)
Provedor de dados: 56
Autores: Villar,L.M.; Amado,L.A.; Gaspar,A.M.C.
Hepatitis A virus (HAV) replicates relatively slowly in cell culture without a cytopathic effect, a fact that limits the use of tissue culture assays. The radioimmunofocus assay is the standard method for HAV titration, although it is labor intensive and requires the use of radioisotopes. A simple, rapid and objective infectivity assay based on an in situ enzyme immunoassay (EIA) is described here for a Brazilian cell culture-adapted HAV strain (HAF-203). The assay uses a peroxidase-labeled polyclonal antibody to fixed monolayers as an indicator of infection. EIA may be completed within 7 days using serial 5-fold dilutions of the virus, yielding a titer of 5.024 log 50% tissue culture infective dose (TCID50)/ml for HAF-203. This technique had a detection...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis A virus; Virus titration; Enzyme immunoassay.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700011

Optical coherence tomography imaging of the basal ganglia: feasibility and brief review
Provedor de dados: 56
Autores: Lopez,W. O. Contreras; Ângelos,J. S.; Martinez,R. C. R.; Takimura,C. K.; Teixeira,M. J.; Lemos Neto,P. A.; Fonoff,E. T.
Optical coherence tomography (OCT) is a promising medical imaging technique that uses light to capture real-time cross-sectional images from biological tissues in micrometer resolution. Commercially available optical coherence tomography systems are employed in diverse applications, including art conservation and diagnostic medicine, notably in cardiology and ophthalmology. Application of this technology in the brain may enable distinction between white matter and gray matter, and obtainment of detailed images from within the encephalon. We present, herein, the in vivo implementation of OCT imaging in the rat brain striatum. For this, two male 60-day-old rats (Rattus norvegicus, Albinus variation, Wistar) were stereotactically implanted with guide cannulas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Optical coherence tomography (OCT); Basal ganglia; Rat brain; Brain imaging; Histology; Thalamus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201156

Cytogenetic studies of Brazilian pediatric myelodysplastic syndrome cases: challenges and difficulties in a large and emerging country
Provedor de dados: 56
Autores: Velloso,E.D.R.P.; Chauffaille,M.L.; Peliçario,L.M.; Tanizawa,R.S.S.; Toledo,S.R.C.; Gaiolla,R.D.; Lopes,L.F.
Myelodysplastic syndromes (MDS) and juvenile myelomonocytic leukemia (JMML) are rare hematopoietic stem cell diseases affecting children. Cytogenetics plays an important role in the diagnosis of these diseases. We report here the experience of the Cytogenetic Subcommittee of the Brazilian Cooperative Group on Pediatric Myelodysplastic Syndromes (BCG-MDS-PED). We analyzed 168 cytogenetic studies performed in 23 different cytogenetic centers; 84 of these studies were performed in patients with confirmed MDS (primary MDS, secondary MDS, JMML, and acute myeloid leukemia/MDS+Down syndrome). Clonal abnormalities were found in 36.9% of the MDS cases and cytogenetic studies were important for the detection of constitutional diseases and for differential diagnosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Childhood myelodysplastic syndromes; Juvenile myelomonocytic leukemia; Cytogenetics; Karyotype; Diagnosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100085

Use of the caffeine-halothane contracture test for the diagnosis of malignant hyperthermia in Brazil
Provedor de dados: 56
Autores: Sudo,R.T.; Cunha,L.B.P.; Carmo,P.L.; Matos,A.R.; Trachez,M.M.; Cardoso,L.A.M.; Aguiar,M.I.S.; Abreu,A.V.; Zapata-Sudo,G.
Malignant hyperthermia (MH) is a pharmacogenetic disease triggered by volatile anesthetics and succinylcholine. Deaths due to MH have been reported in Brazil. The first Malignant Hyperthermia Diagnostic and Research Center in Latin America was inaugurated in 1993 at the Federal University of Rio de Janeiro, Brazil. The center followed the diagnostic protocols of the North America MH Group, in which the contractures of biopsies from the vastus lateralis muscle are analyzed after exposure to caffeine and halothane (CHCT). CHCT was performed in individuals who survived, their relatives and those with signs/symptoms somewhat related to MH susceptibility (MHS). Here, we report data from 194 patients collected over 16 years. The Southeast (N = 110) and South (N...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malignant hyperthermia; Diagnosis; Skeletal muscle; Anesthesia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600005

Fecal estradiol and progesterone metabolite levels in the three-toed sloth (Bradypus variegatus)
Provedor de dados: 56
Autores: Mühlbauer,M.; Duarte,D.P.F.; Gilmore,D.P.; da Costa,C.P.
The present study was carried out to assess the possibility of measuring fecal steroid hormone metabolites as a noninvasive technique for monitoring reproductive function in the three-toed sloth, Bradypus variegatus. Levels of the estradiol (E2) and progesterone (P4) metabolites were measured by radioimmunoassay in fecal samples collected over 12 weeks from 4 captive female B. variegatus sloths. The validation of the radioimmunoassay for evaluation of fecal steroid metabolites was carried out by collecting 10 blood samples on the same day as defecation. There was a significant direct correlation between the plasma and fecal E2 and P4 levels (P &lt; 0.05, Pearson's test), thereby validating this noninvasive technique for the study of the estrous cycle in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sloth; Fecal estrogen and progestogen metabolites; Noninvasive monitoring; Estrous cycle; Fecal hormones.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200017

Salivary cortisol as a tool for physiological studies and diagnostic strategies
Provedor de dados: 56
Autores: Castro,M.; Elias,P.C.L.; Martinelli Jr.,C.E.; Antonini,S.R.R.; Santiago,L.; Moreira,A.C.
Salivary cortisol is an index of plasma free cortisol and is obtained by a noninvasive procedure. We have been using salivary cortisol as a tool for physiological and diagnostic studies, among them the emergence of circadian rhythm in preterm and term infants. The salivary cortisol circadian rhythm in term and premature infants was established between 8 and 12 postnatal weeks. In the preterm infants the emergence of circadian rhythm was parallel to the onset of sleep rhythm. We also studied the use of salivary cortisol for screening for Cushing's syndrome (CS) in control and obese outpatients based on circadian rhythm and the overnight 1 mg dexamethasone (DEX) suppression test. Salivary cortisol was suppressed to less than 100 ng/dl after 1 mg DEX in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salivary cortisol; Ontogeny; Cushing's syndrome.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000006

Effect of the human leukocyte antigen HLA-DRB1 and anti-cyclic citrullinated peptide on the outcome of rheumatoid arthritis patients
Provedor de dados: 56
Autores: Farouk,H.M.; Mansour,H.E.; Rahman,S.A.; Mostafa,A.A.; Shamy,H.A.; Zarouk,W.A.
Our objective was to determine whether the presence of the human leukocyte antigen HLA-DRB1 locus is associated with production of anti-cyclic citrullinated peptide antibodies (anti-CCP Abs) and to what extent they are associated with increased susceptibility to and severity of rheumatoid arthritis (RA) in Egyptian patients. Twenty-nine RA patients gave informed consent to participate in a case-control study that was approved by the Ain Shams University Medical Ethics Committee. RA disease activity and severity were determined using the simplified disease activity index and Larsen scores, respectively. We used a wide scale national study on the pattern of HLA typing in normal Egyptians as a control study. Anti-CCP Abs and HLA-DRB1 typing were determined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA-DRB1; Anti-CCP Ab; Rheumatoid arthritis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900010

Detection of specific IgE antibodies in parasite diseases
Provedor de dados: 56
Autores: Souza-Atta,M.L.B.; Araújo,M.I.; D'Oliveira Júnior,A.; Ribeiro-de-Jesus,A.; Almeida,R.P.; Atta,A.M.; Carvalho,E.M.
Activation of Th1 or Th2 cells is associated with production of specific immunoglobulin isotypes, offering the opportunity to use antibody measurement for evaluation of T cell function. Schistosomiasis and visceral leishmaniasis are diseases associated with Th2 activation. However, an IgE response is not always detected in these patients. In the present study we evaluated specific IgE antibodies to S. mansoni and L. chagasi antigens by ELISA after depletion of serum IgG with protein G immobilized on Sepharose beads or RF-absorbent (purified sheep IgG antibodies anti-human IgG). In schistosomiasis patients, specific IgE to SWAP antigen was demonstrable in only 10 of 21 patients (48%) (mean absorbance ± SD = 0.102 ± 0.195) when unabsorbed serum was used....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: IgE antibodies; Immunoassays; Schistosomiasis mansoni; Visceral leishmaniasis; Kala-azar.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900008

Effects of endurance training on reduction of plasma glucose during high intensity constant and incremental speed tests in Wistar rats
Provedor de dados: 56
Autores: Abreu,P.; Vitzel,K.F.; Monteiro,I.C.C.R.; Lima,T.I.; Queiroz,A.N.; Leal-Cardoso,J.H.; Hirabara,S.M.; Ceccatto,V.M.
The aim of this research was to investigate the effects of endurance training on reduction of plasma glucose during high intensity constant and incremental speed tests in Wistar rats. We hypothesized that plasma glucose might be decreased in the exercised group during heavy (more intense) exercise. Twenty-four 10-week-old male Wistar rats were randomly assigned to sedentary and exercised groups. The prescription of endurance exercise training intensity was determined as 60% of the maximum intensity reached at the incremental speed test. The animals were trained by running on a motorized treadmill, five days/week for a total period of 67 weeks. Plasma glucose during the constant speed test in the exercised group at 20 m/min was reduced at the 14th, 21st and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise; Skeletal muscle; Liver; Blood glucose; Training.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100602

REGγ is a strong candidate for the regulation of cell cycle, proliferation and the invasion by poorly differentiated thyroid carcinoma cells
Provedor de dados: 56
Autores: Zhang,M.; Gan,L.; Ren,G.S.
REGγ is a proteasome activator that facilitates the degradation of small peptides. Abnormally high expression of REGγ has been observed in thyroid carcinomas. The purpose of the present study was to explore the role of REGγ in poorly differentiated thyroid carcinoma (PDTC). For this purpose, small interfering RNA (siRNA) was introduced to down-regulate the level of REGγ in the PDTC cell line SW579. Down-regulation of REGγ at the mRNA and protein levels was confirmed by RT-PCR and Western blot analyses. FACS analysis revealed cell cycle arrest at the G1/S transition, the MTT assay showed inhibition of cell proliferation, and the Transwell assay showed restricted cell invasion. Furthermore, the expression of the p21 protein was increased, the expression of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: REGγ; PDTC; Cell cycle; Cell proliferation; SW579 cell line; Thyroid carcinoma.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500012

Factor structure of Bech's version of the Brief Psychiatric Rating Scale in Brazilian patients
Provedor de dados: 56
Autores: Crippa,J.A.S.; Sanches,R.F.; Hallak,J.E.C.; Loureiro,S.R.; Zuardi,A.W.
The objective of the present study was to evaluate the factor structure of Bech's version of the Brief Psychiatric Rating Scale (BPRS), translated into Portuguese. The BPRS was administered to a heterogeneous group of psychiatric inpatients (N = 98) and outpatients (N = 62) in a University Hospital. Each patient was evaluated from one to eight times. The interval between consecutive interviews was one week for the inpatients and one month for the outpatients. The results were submitted to factorial analysis. The internal consistency of the total scale and of each factor was also estimated. Factorial analysis followed by normalized orthogonal rotation (Varimax) yielded four factors: Withdrawal-Retardation, Thinking Disorder, Anxious-Depression and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Brief Psychiatric Rating Scale; Psychiatric status rating scales; Psychometrics; Portuguese version; Factorial analysis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000014

Nephrotoxicity of Bence-Jones proteins: interference in renal epithelial cell acidification
Provedor de dados: 56
Autores: Nicastri,A.L.; Prado,M.J.B.A.; Dominguez,W.V.; Prado,E.B.A.
The aim of the present study was to evaluate the acidification of the endosome-lysosome system of renal epithelial cells after endocytosis of two human immunoglobulin lambda light chains (Bence-Jones proteins, BJP) obtained from patients with multiple myeloma. Renal epithelial cell handling of two BJP (neutral and acidic BJP) was evaluated by rhodamine fluorescence. Renal cells (MDCK) were maintained in culture and, when confluent, were incubated with rhodamine-labeled BJP for different periods of time. Photos were obtained with a fluorescence microscope (Axiolab-Zeiss). Labeling density was determined on slides with a densitometer (Shimadzu Dual-Wavelength Flying-Spot Scanner CS9000). Endocytosis of neutral and acidic BJP was correlated with acidic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bence-Jones proteins; Renal cell acidification; Endocytosis; Lambda light chain protein.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300011

Glycyrrhizin attenuates endotoxin- induced acute liver injury after partial hepatectomy in rats
Provedor de dados: 56
Autores: Tang,B.; Qiao,H.; Meng,F.; Sun,X.
Massive hepatectomy associated with infection induces liver dysfunction, or even multiple organ failure and death. Glycyrrhizin has been shown to exhibit anti-oxidant and anti-inflammatory activities. The aim of the present study was to investigate whether glycyrrhizin could attenuate endotoxin-induced acute liver injury after partial hepatectomy. Male Wistar rats (6 to 8 weeks old, weighing 200-250 g) were randomly assigned to three groups of 24 rats each: sham, saline and glycyrrhizin. Rats were injected intravenously with lipopolysaccharide (LPS) 24 h after 70% hepatectomy. Glycyrrhizin, pre-administered three times with 24 h intervals 48 h before hepatectomy, prolonged the survival of rats submitted to partial hepatectomy and LPS injection, compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycyrrhizin; Lipopolysaccharide; Hepatectomy; Inflammatory cytokine; Apoptosis; Endotoxemia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200007

Karyological, biochemical, and physiological aspects of Callophysus macropterus (Siluriformes, Pimelodidae) from the Solimões and Negro Rivers (Central Amazon)
Provedor de dados: 56
Autores: Ramirez-Gil,H.; Feldberg,E.; Almeida-Val,V.M.F.; Val,A.L.
Karyological characteristics, i.e., diploid number, chromosome morphology and nucleolus organizer regions (NORs), biochemical characteristics, i.e., electrophoretic analysis of blood hemoglobin and the tissue enzymes lactate dehydrogenase (LDH), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), and phosphoglucose isomerase (PGI), and physiological characteristics, i.e., relative concentration of hemoglobin and intraerythrocytic concentrations of organic phosphates were analyzed for the species Callophysus macropterus collected from Marchantaria Island (white water system - Solimões River) and Anavilhanas Archipelago (black water system - Negro River). Karyological and biochemical data did not reveal significant differences between specimens...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Callophysus; Fish; Amazon; Population genetics; Adaptation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100014

Use of neuron-specific enolase for assessing the severity and outcome of neurological disorders in patients
Provedor de dados: 56
Autores: Lima,J.E.; Takayanagui,O.M.; Garcia,L.V.; Leite,J.P.
Neuron-specific enolase (NSE) is a glycolytic enzyme present almost exclusively in neurons and neuroendocrine cells. NSE levels in cerebrospinal fluid (CSF) are assumed to be useful to estimate neuronal injury and clinical outcome of patients with serious clinical manifestations such as those observed in stroke, head injury, anoxic encephalopathy, encephalitis, brain metastasis, and status epilepticus. We compared levels of NSE in serum (sNSE) and in CSF (cNSE) among four groups: patients with meningitis (N = 11), patients with encephalic injuries associated with impairment of consciousness (ENC, N = 7), patients with neurocysticercosis (N = 25), and normal subjects (N = 8). Albumin was determined in serum and CSF samples, and the albumin quotient was used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuronal damage; Neuron-specific enolase; Cerebrospinal fluid; Albumin quotient.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100003

Combined effects of diethylpropion and alcohol on locomotor activity of mice: participation of the dopaminergic and opioid systems
Provedor de dados: 56
Autores: Gevaerd,M.S.; Sultowski,E.T.; Takahashi,R.N.
The widespread consumption of anorectics and combined anorectic + alcohol misuse are problems in Brazil. In order to better understand the interactive effects of ethanol (EtOH) and diethylpropion (DEP) we examined the locomotion-activating effects of these drugs given alone or in combination in mice. We also determined whether this response was affected by dopamine (DA) or opioid receptor antagonists. A total of 160 male Swiss mice weighing approximately 30 g were divided into groups of 8 animals per group. The animals were treated daily for 7 consecutive days with combined EtOH + DEP (1.2 g/kg and 5.0 mg/kg, ip), EtOH (1.2 g/kg, ip), DEP (5.0 mg/kg, ip) or the control solution coadministered with the DA antagonist haloperidol (HAL, 0.075 mg/kg, ip), the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diethylpropion; Ethanol; Locomotor activity; Dopamine; Opioid; Mice.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200015

The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response
Provedor de dados: 56
Autores: Almeida,S.R.; Lopes,J.D.
In the present study we evaluated T cell proliferation and Th lymphokine patterns in response to gp43 from Paracoccidioides brasiliensis presented by isolated dendritic cells from susceptible and resistant mice. T cell proliferation assays showed that dendritic cells from susceptible mice were less efficient than those from resistant mice. The pattern of T cell lymphokines stimulated by dendritic cells was always Th1, although the levels of IL-2 and IFN-gamma were lower in T cell cultures from susceptible mice. To determie whether different antigen-presenting cells such as macrophages and dendritic cells stimulated different concentrations of Th1 lymphokines, the production of IFN-gamma and IL-2 was measured. It was observed that dendritic cells were more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendritic cells; Paracoccidioidomycosis; Paracoccidioides brasiliensis; Th1/Th2.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400014

Comparison of the quantitative competitive and semiquantitative RT-PCR methods for the determination of interferon-gamma mRNA levels in AIDS-free HIV-infected individuals
Provedor de dados: 56
Autores: Barabás,É.; Nagy,K.; Várkonyi,V.; Horváth,A.
IFN-gamma mRNA expression was evaluated in nonstimulated peripheral blood mononuclear cells (PBMC) of HIV-infected and seronegative individuals using quantitative competitive and semiquantitative RT-PCR and the sensitivity of these methods was compared. A significant correlation was found between quantitative competitive and semiquantitative RT-PCR in samples of both HIV-seronegative (P = 0.004) and HIV-infected individuals (P = 0.0004). PBMC from HIV-infected individuals presented a remarkable increase of IFN-gamma mRNA expression, as determined by both types of RT-PCR methods. Semiquantitative RT-PCR even without an internal standard is also acceptable for measuring cytokine mRNA expression, but less reliable if small amounts are quantified. Moreover, we...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Quantitative competitive RT-PCR; Semiquantitative RT-PCR; IFN-gamma mRNA; HIV.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000006

Genotyping of methicillin-resistant Staphylococcus aureus isolates obtained in the Northeast region of Brazil
Provedor de dados: 56
Autores: Sousa-Junior,F.C. de; Silva-Carvalho,M.C.; Fernandes,M.J.B.C.; Vieira,M.F.P.; Pellegrino,F.L.P.C.; Figueiredo,A.M.S.; Melo,M.C.N. de; Milan,E.P.
Methicillin-resistant Staphylococcus aureus (MRSA) is a major agent of hospital infections worldwide. In Brazil, a multiresistant MRSA lineage (ST239-SCCmecIIIA), the so-called Brazilian epidemic clone (BEC), has predominated in all regions. However, an increase in nosocomial infections caused by non-multiresistant MRSA clones has recently been observed. In the present study, 45 clinical isolates of MRSA obtained from a university hospital located in Natal city, Brazil, were identified by standard laboratory methods and molecularly characterized using staphylococcal chromosome cassette mec (SCCmec) typing and pulsed-field gel electrophoresis. Antimicrobial susceptibility testing was carried out using CLSI methods. The MRSA isolates studied displayed a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methicillin-resistant Staphylococcus aureus; MRSA; Antimicrobial resistance; Brazilian epidemic clone; Pediatric clone; SCCmecIV.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000002

Assessment of the progressive nature of cell damage in the pilocarpine model of epilepsy
Provedor de dados: 56
Autores: Covolan,L.; Mello,L.E.
Pilocarpine-induced (320 mg/kg, ip) status epilepticus (SE) in adult (2-3 months) male Wistar rats results in extensive neuronal damage in limbic structures. Here we investigated whether the induction of a second SE (N = 6) would generate damage and cell loss similar to that seen after a first SE (N = 9). Counts of silver-stained (indicative of cell damage) cells, using the Gallyas argyrophil III method, revealed a markedly lower neuronal injury in animals submitted to re-induction of SE compared to rats exposed to a single episode of pilocarpine-induced SE. This effect could be explained as follows: 1) the first SE removes the vulnerable cells, leaving behind resistant cells that are not affected by the second SE; 2) the first SE confers increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell injury; Status epilepticus; Epilepsy; Pilocarpine; Spontaneous seizures.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700010

Rat visceral yolk sac cells: viability and expression of cell markers during maternal diabetes
Provedor de dados: 56
Autores: Aires,M.B.; Santos,J.R.A.; Souza,K.S.; Farias,P.S.; Santos,A.C.V.; Fioretto,E.T.; Maria,D.A.
The function of the visceral yolk sac (VYS) is critical for embryo organogenesis until final fetal development in rats, and can be affected by conditions such as diabetes. In view of the importance of diabetes during pregnancy for maternal and neonatal health, the objective of this study was to assess fetal weight, VYS cell markers, and viability in female Wistar rats (200-250 g) with induced diabetes (alloxan, 37 mg/kg) on the 8th gestational day (gd 8). At gd 15, rats from control (n=5) and diabetic (n=5) groups were anesthetized and laparotomized to remove the uterine horns for weighing of fetuses and collecting the VYS. Flow cytometry was used for characterizing VYS cells, and for determining mitochondrial activity, cell proliferation, DNA ploidy, cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral yolk sac; Diabetes; Embryo; Pregnancy; Cell markers.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800676

Visual and automatic classification of the cyclic alternating pattern in electroencephalography during sleep
Provedor de dados: 56
Autores: Largo,R.; Lopes,M.C.; Spruyt,K.; Guilleminault,C.; Wang,Y.P.; Rosa,A.C.
Cyclic alternating pattern (CAP) is a neurophysiological pattern that can be visually scored by international criteria. The aim of this study was to verify the feasibility of visual CAP scoring using only one channel of sleep electroencephalogram (EEG) to evaluate the inter-scorer agreement in a variety of recordings, and to compare agreement between visual scoring and automatic scoring systems. Sixteen hours of single-channel European data format recordings from four different sleep laboratories with either C4-A1 or C3-A2 channels and with different sampling frequencies were used in this study. Seven independent scorers applied visual scoring according to international criteria. Two automatic blind scorings were also evaluated. Event-based inter-scorer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclic alternating pattern; Sleep; Visual scoring; Automatic scoring.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300602

Beneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese rats
Provedor de dados: 56
Autores: Banin,R.M.; Hirata,B.K.S.; Andrade,I.S.; Zemdegs,J.C.S.; Clemente,A.P.G.; Dornellas,A.P.S.; Boldarine,V.T.; Estadella,D.; Albuquerque,K.T.; Oyama,L.M.; Ribeiro,E.B.; Telles,M.M.
Ginkgo biloba extract (GbE) has been indicated as an efficient medicine for the treatment of diabetes mellitus type 2. It remains unclear if its effects are due to an improvement of the insulin signaling cascade, especially in obese subjects. The aim of the present study was to evaluate the effect of GbE on insulin tolerance, food intake, body adiposity, lipid profile, fasting insulin, and muscle levels of insulin receptor substrate 1 (IRS-1), protein tyrosine phosphatase 1B (PTP-1B), and protein kinase B (Akt), as well as Akt phosphorylation, in diet-induced obese rats. Rats were fed with a high-fat diet (HFD) or a normal fat diet (NFD) for 8 weeks. After that, the HFD group was divided into two groups: rats gavaged with a saline vehicle (HFD+V), and rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginkgo biloba extract; Obesity; Insulin tolerance; Body adiposity; Akt phosphorylation; Rats.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900780

The Brazilian investment in science and technology
Provedor de dados: 56
Autores: Pinheiro-Machado,R.; de Oliveira,P.L.
An analysis of Brazilian federal expenditures in science and technology is presented is this study. The 1990-1999 data were compiled from records provided by two federal agencies (MCT and CNPq) responsible for managing most of the national budget related to these activities. The results indicate that the federal investments in Brazilian science and technology stagnated during the last decade (US$ 2.32 billion in 1990, US$ 2.39 billion in 1996, and US$ 2.36 billion in 1999). In contrast, a great increase in private investments in research was acknowledged both by industry and by the government during the same period, from US$ 2.12 to US$ 4.64 billion. However, this investment did not result in an increase in invention patents granted to residents (492 in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Science and technology; Research and development; Patents; Technological innovation; Scientific publication.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200003

Evaluation of cardiovascular toxicity of carbon nanotubes functionalized with sodium hyaluronate in oral regenerative medicine
Provedor de dados: 56
Autores: Joviano-Santos,J.V.; Sá,M.A.; De Maria,M.L.A.; Almeida,T.C.S.; Geraldo,V.; Oliveira,S.; Ladeira,L.O.; Ferreira,A.J.
It has been demonstrated that carbon nanotubes (CNTs) associated with sodium hyaluronate (HY-CNTs) accelerate bone repair in the tooth sockets of rats. Before clinical application of HY-CNTs, it is important to assess their biocompatibility. Moreover, cardiac toxicity may be caused by the translocation of these particles to the blood stream. The aim of this study was to evaluate possible changes in cardiovascular function in male Wistar rats whose tooth sockets were treated with either CNTs or HY-CNTs (100 μg/mL, 0.1 mL). Blood pressure and heart rate were monitored in conscious rats 7 days after treatment. Cardiac function was evaluated using the Langendorff perfusion technique. The data showed no changes in blood pressure or heart rate in rats treated...
Palavras-chave: Cardiac function; Blood pressure; Carbon nanotubes; Nanotechnology; Bone repair; Tooth sockets.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700560

Investigation on the nasal airflow characteristics of anterior nasal cavity stenosis
Provedor de dados: 56
Autores: Wang,T.; Chen,D.; Wang,P.H.; Chen,J.; Deng,J.
We used a computational fluid dynamics (CFD) model to study the inspiratory airflow profiles of patients with anterior nasal cavity stenosis who underwent curative surgery, by comparing pre- and postoperative airflow characteristics. Twenty patients with severe anterior nasal cavity stenosis, including one case of bilateral stenosis, underwent computed tomography (CT) scans for CFD modelling. The pre- and postoperative airflow characteristics of the nasal cavity were simulated and analyzed. The narrowest area of the nasal cavity in all 20 patients was located within the nasal valve area, and the mean cross-sectional area increased from 0.39 cm2 preoperative to 0.78 cm2 postoperative (P&lt;0.01). Meanwhile, the mean airflow velocity in the nasal valve area...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anterior nasal cavity stenosis; Computational fluid dynamics; Nasal valve area; Airflow characteristics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900704

Determination of CYP2D6 *3, *4, and *10 frequency in women with breast cancer in São Luís, Brazil, and its association with prognostic factors and disease-free survival
Provedor de dados: 56
Autores: Martins,D.M.F.; Vidal,F.C.B.; Souza,R.D.M.; Brusaca,S.A.; Brito,L.M.O.
The CYP2D6 enzyme is crucial for the metabolism of tamoxifen. The CYP2D6 gene is highly polymorphic, and individuals can be extensive, intermediate, or poor tamoxifen metabolizers. The aim of this study was to determine the frequencies of the CYP2D6 *3, *4, and *10 alleles in women with breast cancer who were treated with tamoxifen and analyze the association of enzyme activity with prognostic factors and disease-free survival. We observed a high frequency of CYP2D6 *10, with an allelic frequency of 0.14 (14.4%). The *3 allele was not present in the studied population, and *4 had an allelic frequency of 0.13 (13.8%). We conclude that patients with reduced CYP2D6 activity did not present worse tumor characteristics or decreased disease-free survival than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tamoxifen; Cytochrome P450 2D6; Breast cancer; Genetic polymorphisms; Prognostic factors; Disease-free survival.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001101008

The APOB rs693 polymorphism impacts the lipid profile of Brazilian older adults
Provedor de dados: 56
Autores: Alves,E.S.; Henriques,A.D.; Tonet-Furioso,A.C.; Paula,R.S.; Gomes,L.O.; Moraes,C.F.; Nóbrega,O.T.
The apolipoprotein B (APOB) gene contains several polymorphic sites described as risk modifiers for cardiovascular events. The objective of this study was to verify the association of the classic APOB Xba I polymorphism (rs693) with atherosclerotic risk factors in a segment of the Brazilian elderly population considering their usual dietary intake. Clinical and biochemical characteristics as well as total caloric and fat intake data were determined from 644 elderly individuals. Polymorphism analysis was performed by conventional polymerase chain reaction followed by enzyme restriction. Statistical analyses compared measures and proportions according to different APOB genotypic combinations. Statistically significant association was found between Xba I...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein B; Genetic polymorphism; Elderly; Hyperlipidemia; Diet.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300608

Screening potential prognostic biomarkers of long non-coding RNAs for predicting the risk of chronic kidney disease
Provedor de dados: 56
Autores: Li,Na; Cui,Yan; Yin,Min; Liu,Feng.
Not much is known about the roles of long non-coding RNAs (lncRNAs) for chronic kidney disease (CKD). In this study, we included CKD patient cohorts and normal controls as a discovery cohort to identify putative lncRNA biomarkers associated with CKD. We first compared the lncRNA expression profiles of CKD patients with normal controls, and identified differentially expressed lncRNAs and mRNAs. Co-expression network based on the enriched differentially expressed mRNAs and lncRNAs was constructed using WGCNA to identify important modules related to CKD. A lncRNA-miRNA-mRNA pathway network based on the hub lncRNAs and mRNAs, related miRNAs, and overlapping pathways was further constructed to reveal putative biomarkers. A total of 821 significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Long non-coding RNAs; Chronic kidney disease; WGCNA; Prognostic biomarkers.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100608

Signal transduction induced in Trypanosoma cruzi metacyclic trypomastigotes during the invasion of mammalian cells
Provedor de dados: 56
Autores: Yoshida,N.; Favoreto Jr.,S.; Ferreira,A.T.; Manque,P.M.
Penetration of Trypanosoma cruzi into mammalian cells depends on the activation of the parasite's protein tyrosine kinase and on the increase in cytosolic Ca2+ concentration. We used metacyclic trypomastigotes, the T. cruzi developmental forms that initiate infection in mammalian hosts, to investigate the association of these two events and to identify the various components of the parasite signal transduction pathway involved in host cell invasion. We have found that i) both the protein tyrosine kinase activation, as measured by phosphorylation of a 175-kDa protein (p175), and Ca2+ mobilization were induced in the metacyclic forms by the HeLa cell extract but not by the extract of T. cruzi-resistant K562 cells; ii) treatment of parasites with the tyrosine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Signal transduction; Metacyclic trypomastigotes; Protein kinase; Calcium response; Cell invasion.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300003

Behavioral effects of Bj-PRO-7a, a proline-rich oligopeptide from Bothrops jararaca venom
Provedor de dados: 56
Autores: Turones,L.C.; Cruz,K.R. da; Camargo-Silva,G.; Reis-Silva,L.L.; Graziani,D.; Ferreira,P.M.; Galdino,P.M.; Pedrino,G.R.; Santos,R.; Costa,E.A.; Ianzer,D.; Xavier,C.H.
The heptapeptide Bj-PRO-7a, isolated and identified from Bothrops jararaca (Bj) venom, produces antihypertensive and other cardiovascular effects that are independent on angiotensin converting enzyme inhibition, possibly relying on cholinergic muscarinic receptors subtype 1 (M1R). However, whether Bj-PRO-7a acts upon the central nervous system and modifies behavior is yet to be determined. Therefore, the aims of this study were: i) to assess the effects of acute administration of Bj-PRO-7a upon behavior; ii) to reveal mechanisms involved in the effects of Bj-PRO-7a upon locomotion/exploration, anxiety, and depression-like behaviors. For this purpose, adult male Wistar (WT, wild type) and spontaneous hypertensive rats (SHR) received intraperitoneal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bj-PRO-7a; Snake venom; Neuroactive compounds; Anxiety; Depression; Behavior.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100606

Role of Tamm-Horsfall protein and uromodulin in calcium oxalate crystallization
Provedor de dados: 56
Autores: Carvalho,M.; Mulinari,R.A.; Nakagawa,Y.
One of the defenses against nephrolithiasis is provided by macromolecules that modulate the nucleation, growth, aggregation and retention of crystals in the kidneys. The aim of the present study was to determine the behavior of two of these proteins, Tamm-Horsfall and uromodulin, in calcium oxalate crystallization in vitro. We studied a group of 10 male stone formers who had formed at least one kidney stone composed of calcium oxalate. They were classified as having idiopathic nephrolithiasis and had no well-known metabolic risk factors involved in kidney stone pathogenesis. Ten normal men were used as controls, as was a group consisting of five normal women and another consisting of five pregnant women. Crystallization was induced by a fixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tamm-Horsfall protein; Uromodulin; Calcium oxalate; Nephrolithiasis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000009

Involvement of miR-30c in resistance to doxorubicin by regulating YWHAZ in breast cancer cells
Provedor de dados: 56
Autores: Fang,Y.; Shen,H.; Cao,Y.; Li,H.; Qin,R.; Chen,Q.; Long,L.; Zhu,X.L.; Xie,C.J.; Xu,W.L.
MicroRNAs (miRNAs) are small RNA molecules that modulate gene expression implicated in cancer, which play crucial roles in diverse biological processes, such as development, differentiation, apoptosis, and proliferation. The aim of this study was to investigate whether miR-30c mediated the resistance of breast cancer cells to the chemotherapeutic agent doxorubicin (ADR) by targeting tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ). miR-30c was downregulated in the doxorubicin-resistant human breast cancer cell lines MCF-7/ADR and MDA-MB-231/ADR compared with their parental MCF-7 and MDA-MB-231 cell lines, respectively. Furthermore, we observed that transfection of an miR-30c mimic significantly suppressed the ability of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer cells; MiR-30c; YWHAZ; Doxorubicin resistance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100060

Hypoxic-ischemic injury decreases anxiety-like behavior in rats when associated with loss of tyrosine-hydroxylase immunoreactive neurons of the substantia nigra
Provedor de dados: 56
Autores: Ming-Yan,Hei; Luo,Ya-Li; Zhang,Xiao-Chun; Liu,Hong; Gao,Ru; Wu,Jing-Jiang.
Neonatal Sprague-Dawley rats were randomly divided into normal control, mild hypoxia-ischemia (HI), and severe HI groups (N = 10 in each group at each time) on postnatal day 7 (P7) to study the effect of mild and severe HI on anxiety-like behavior and the expression of tyrosine hydroxylase (TH) in the substantia nigra (SN). The mild and severe HI groups were exposed to hypoxia (8% O2/92% N2) for 90 and 150 min, respectively. The elevated plus-maze (EPM) test was performed to assess anxiety-like behavior by measuring time spent in the open arms (OAT) and OAT%, and immunohistochemistry was used to determine the expression of TH in the SN at P14, P21, and P28. OAT and OAT% in the EPM were significantly increased in both the mild (1.88-, 1.99-, and 2.04-fold,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anxiety; Tyrosine hydroxylase; Substantia nigra; Hypoxia-ischemia.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100003

Ethical research as the target of animal extremism: an international problem
Provedor de dados: 56
Autores: Michael Conn,P.; Rantin,F.T.
Animal extremism has been increasing worldwide; frequently researchers are the targets of actions by groups with extreme animal rights agendas. Sometimes this targeting is violent and may involve assaults on family members or destruction of property. In this article, we summarize recent events and suggest steps that researchers can take to educate the public on the value of animal research both for people and animals
Tipo: Info:eu-repo/semantics/other
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200001

Vascular changes after cardiopulmonary bypass and ischemic cardiac arrest: roles of nitric oxide synthase and cyclooxygenase
Provedor de dados: 56
Autores: Sellke,F.W.
Cardiac surgery involving ischemic arrest and extracorporeal circulation is often associated with alterations in vascular reactivity and permeability due to changes in the expression and activity of isoforms of nitric oxide synthase and cyclooxygenase. These inflammatory changes may manifest as systemic hypotension, coronary spasm or contraction, myocardial failure, and dysfunction of the lungs, gut, brain and other organs. In addition, endothelial dysfunction may increase the occurrence of late cardiac events such as graft thrombosis and myocardial infarction. These vascular changes may lead to increased mortality and morbidity and markedly lengthen the time of hospitalization and cost of cardiac surgery. Developing a better understanding of the vascular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide synthase; Cyclooxygenase; Heart surgery; Endothelium; Microcirculation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100004

Follicular helper T cell in immunity and autoimmunity
Provedor de dados: 56
Autores: Mesquita Jr,D.; Cruvinel,W.M.; Resende,L.S.; Mesquita,F.V.; Silva,N.P.; Câmara,N.O.S.; Andrade,L.E.C.
The traditional concept that effector T helper (Th) responses are mediated by Th1/Th2 cell subtypes has been broadened by the recent demonstration of two new effector T helper cells, the IL-17 producing cells (Th17) and the follicular helper T cells (Tfh). These new subsets have many features in common, such as the ability to produce IL-21 and to express the IL-23 receptor (IL23R), the inducible co-stimulatory molecule ICOS, and the transcription factor c-Maf, all of them essential for expansion and establishment of the final pool of both subsets. Tfh cells differ from Th17 by their ability to home to B cell areas in secondary lymphoid tissue through interactions mediated by the chemokine receptor CXCR5 and its ligand CXCL13. These CXCR5+ CD4+ T cells are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autoimmune diseases; Tfh; Lymphoid tissue; Humoral immunity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500302

Impaired beta-adrenergic response and decreased L-type calcium current of hypertrophied left ventricular myocytes in postinfarction heart failure
Provedor de dados: 56
Autores: Saraiva,R.M.; Chedid,N.G.B.; Quintero H.,C.C.; Díaz G.,L.E.; Masuda,M.O.
Infarct-induced heart failure is usually associated with cardiac hypertrophy and decreased ß-adrenergic responsiveness. However, conflicting results have been reported concerning the density of L-type calcium current (I Ca(L)), and the mechanisms underlying the decreased ß-adrenergic inotropic response. We determined I Ca(L) density, cytoplasmic calcium ([Ca2+]i) transients, and the effects of ß-adrenergic stimulation (isoproterenol) in a model of postinfarction heart failure in rats. Left ventricular myocytes were obtained by enzymatic digestion 8-10 weeks after infarction. Electrophysiological recordings were obtained using the patch-clamp technique. [Ca2+]i transients were investigated via fura-2 fluorescence. ß-Adrenergic receptor density was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ca2+ channel; Heart failure; Hypertrophy; Infarction; Adrenergic receptor.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500012

Penile microvascular endothelial function in hypertensive patients: effects of acute type 5 phosphodiesterase inhibition
Provedor de dados: 56
Autores: Verri,V.; Brandão,A.A.; Tibirica,E.
The primary aim of this study was to evaluate penile endothelial microvascular function in patients with primary arterial hypertension and age-matched normotensive subjects using laser speckle contrast imaging (LSCI). Additionally, we analyzed the acute penile microvascular effects induced by oral phosphodiesterase type 5 inhibitor (sildenafil; SIL) administration. Endothelium-dependent microvascular reactivity was evaluated in the penises and forearms of hypertensive patients (aged 58.8±6.6 years, n=34) and age-matched healthy volunteers (n=33) at rest and 60 min following oral SIL (100 mg) administration. LSCI was coupled with cutaneous acetylcholine (ACh) iontophoresis using increasing anodal currents. Basal penile cutaneous vascular conductance (CVC)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microvascular dysfunction; Hypertension; Laser speckle contrast imaging; Penile microcirculation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300607

Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA
Provedor de dados: 56
Autores: Lima Barros,A.E.; Almeida,A.M.P.; Carvalho Jr.,L.B.; Azevedo,W.M.
We developed an efficient method to prepare a hybrid inorganic-organic composite based on polyvinyl alcohol (PVA) and polysiloxane using the sol-gel disc technique. Antigen obtained from Yersinia pestis was covalently immobilized onto these discs with glutaraldehyde and used as solid phase in ELISA for antibody detection in serum of rabbits experimentally immunized with plague. Using 1.25 µg antigen per disc, a peroxidase conjugate dilution of 1:4,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. These values are similar to those used for PVA-glutaraldehyde discs, plasticized filter paper discs and the polyaniline-Dacron composite discs. This procedure is comparable to that which utilizes the adsorption of the antigen...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Polysiloxane; Polyvinyl alcohol; Solid phase; Immunodetection; Yersinia pestis; ELISA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400008

Effect of protein malnutrition on the glycolytic and glutaminolytic enzyme activity of rat thymus and mesenteric lymph nodes
Provedor de dados: 56
The activity of important glycolytic enzymes (hexokinase, phosphofructokinase, aldolase, phosphohexoseisomerase, pyruvate kinase and lactate dehydrogenase) and glutaminolytic enzymes (phosphate-dependent glutaminase) was determined in the thymus and mesenteric lymph nodes of Wistar rats submitted to protein malnutrition (6% protein in the diet rather than 20%) from conception to 12 weeks after birth. The wet weight (g) of the thymus and mesenteric lymph nodes decreased due to protein malnutrition by 87% (from 0.30 ± 0.05 to 0.04 ± 0.01) and 75% (0.40 ± 0.04 to 0.10 ± 0.02), respectively. The protein content was reduced only in the thymus from 102.3 ± 4.4 (control rats) to 72.6 ± 6.6 (malnourished rats). The glycolytic enzymes were not affected by protein...
Palavras-chave: Protein malnutrition; Glucose metabolism; Glutamine metabolism; Thymus; Lymph nodes.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600003

Visual maps in the adult primate cerebral cortex: some implications for brain development and evolution
Provedor de dados: 56
Autores: Rosa,M.G.P.
In this paper, the topology of cortical visuotopic maps in adult primates is reviewed, with emphasis on recent studies. The observed visuotopic organisation can be summarised with reference to two basic rules. First, adjacent radial columns in the cortex represent partially overlapping regions of the visual field, irrespective of whether these columns are part of the same or different cortical areas. This primary rule is seldom, if ever, violated. Second, adjacent regions of the visual field tend to be represented in adjacent radial columns of a same area. This rule is not as rigid as the first, as many cortical areas form discontinuous, second-order representations of the visual field. A developmental model based on these physiological observations, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Primate; Vision; Receptive fields; Cortical areas; Development; Specification.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001200008

Effect of intraperitoneally administered hydrolyzed whey protein on blood pressure and renal sodium handling in awake spontaneously hypertensive rats
Provedor de dados: 56
Autores: Costa,E.L.; Almeida,A.R.; Netto,F.M.; Gontijo,J.A.R.
The present study evaluated the acute effect of the intraperitoneal (ip) administration of a whey protein hydrolysate (WPH) on systolic arterial blood pressure (SBP) and renal sodium handling by conscious spontaneously hypertensive rats (SHR). The ip administration of WPH in a volume of 1 ml dose-dependently lowered the SBP in SHR 2 h after administration at doses of 0.5 g/kg (0.15 M NaCl: 188.5 ± 9.3 mmHg vs WPH: 176.6 ± 4.9 mmHg, N = 8, P = 0.001) and 1.0 g/kg (0.15 M NaCl: 188.5 ± 9.3 mmHg vs WPH: 163.8 ± 5.9 mmHg, N = 8, P = 0.0018). Creatinine clearance decreased significantly (P = 0.0084) in the WPH-treated group (326 ± 67 µL min-1 100 g body weight-1) compared to 0.15 M NaCl-treated (890 ± 26 µL min-1 100 g body weight-1) and captopril-treated (903...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arterial blood pressure; Hydrolysis; Renal function; Urinary sodium excretion; Whey protein isolate.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200010

Soy milk versus simvastatin for preventing atherosclerosis and left ventricle remodeling in LDL receptor knockout mice
Provedor de dados: 56
Autores: Santos,L.; Davel,A.P.; Almeida,T.I.R.; Almeida,M.R.; Soares,E.A.; Fernandes,G.J.M.; Magalhães,S.F.; Barauna,V.G.; Garcia,J.A.D.
Functional food intake has been highlighted as a strategy for the prevention of cardiovascular diseases by reducing risk factors. In this study, we compared the effects of oral treatment with soy milk and simvastatin on dyslipidemia, left ventricle remodeling and atherosclerotic lesion of LDL receptor knockout mice (LDLr-/-) fed a hyperlipidic diet. Forty 3-month old male LDLr-/- mice were distributed into four groups: control group (C), in which animals received standard diet; HL group, in which animals were fed a hyperlipidic diet; HL+SM or HL+S groups, in which animals were submitted to a hyperlipidic diet plus soy milk or simvastatin, respectively. After 60 days, both soy milk and simvastatin treatment prevented dyslipidemia, atherosclerotic lesion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soy milk Dyslipidemia Oxidative stress Inflammation Atherosclerosis; Simvastatin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300701

Serum leptin concentration during puberty in healthy nonobese adolescents
Provedor de dados: 56
Autores: Brandão,C.M.A.; Lombardi,M.T.; Nishida,S.K.; Hauache,O.M.; Vieira,J.G.H.
Data obtained during the past five years have indicated that there are important age- and gender-based differences in the regulation and action of leptin in humans. To study the physiological changes of leptin during puberty in both sexes, and its relationship with body composition and sexual maturation, we measured leptin concentrations in 175 healthy adolescents (80 girls, 95 boys, 10-18 years of age), representing all pubertal stages. We excluded individuals with a body mass index (BMI) below the 5thor above the 95th percentile relative to age. Serum concentrations of leptin were determined by a monoclonal antibody-based immunofluorimetric assay, developed in our laboratory. Body composition was determined by dual-energy X-ray absorptiometry. Pubertal...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leptin; Pubertal development; Body composition.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000003

The size and prevalence of the cavum septum pellucidum are normal in subjects with panic disorder
Provedor de dados: 56
Autores: Crippa,J.A.S.; Uchida,R.; Busatto,G.F.; Guimarães,F.S.; Del-Ben,C.M.; Zuardi,A.W.; Santos,A.C.; Araújo,D.; McGuire,P.K.; Graeff,F.G.
Panic disorder is thought to involve dysfunction in the septohippocampal system, and the presence of a cavum septum pellucidum might indicate the aberrant development of this system. We compared the prevalence and size of cavum septum pellucidum in 21 patients with panic disorder and in 21 healthy controls by magnetic resonance imaging. The length of the cavum septum pellucidum was measured by counting the number of consecutive 1-mm coronal slices in which it appeared. A cavum septum pellucidum of &gt;6 mm in length was rated as large. There was no significant difference in the proportion of patients (16 of 21 or 76.2%) and controls (18 of 21 or 85.7%) with a cavum septum pellucidum (P = 0.35, Fisher's exact test, one-tailed), and no members of either...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cavum septum pellucidum; Panic disorder; Neuroimaging; Magnetic resonance imaging.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300013

Control of the rat angiotensin I converting enzyme gene by CRE-like sequences
Provedor de dados: 56
Autores: Xavier-Neto,J.; Pereira,A.C.; Oliveira,E.M.; Miyakawa,A.A.; Junqueira,M.L.; Krieger,J.E.
We characterized the role of potential cAMP-responsive elements (CRE) in basal and in induced angiotensin converting enzyme (ACE) gene promoter activity in order to shed light on the regulation of somatic ACE expression. We identified stimulators and repressors of basal expression between 122 and 288 bp and between 415 and 1303 bp upstream from the transcription start site, respectively, using a rabbit endothelial cell (REC) line. These regions also contained elements associated with the response to 8BrcAMP. When screening for CRE motifs we found pCRE, a proximal sequence between 209 and 222 bp. dCRE, a distal tandem of two CRE-like sequences conserved between rats, mice and humans, was detected between 834 and 846 bp. Gel retardation analysis of nuclear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin converting enzyme; Endothelium; CAMP; Cyclic AMP responsive element.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000002

Influence of dexamethasone and weight loss on the regulation of serum leptin levels in obese individuals
Provedor de dados: 56
Autores: Lerario,D.D.G.; Ferreira,S.R.G.; Miranda,W.L.; Chacra,A.R.
The adipocyte hormone leptin is thought to serve as a signal to the central nervous system reflecting the status of fat stores. Serum leptin levels and adipocyte leptin messenger RNA levels are clearly increased in obesity. Nevertheless, the factors regulating leptin production are not fully understood. The aim of this study was to determine the effects of in vivo administration of the synthetic glucocorticoid dexamethasone and weight loss on serum leptin levels in two independent protocols. Twenty-five obese subjects were studied (18 women and 7 men, mean age 26.6 ± 6 years, BMI 31.1 ± 2.5 kg/m², %fat 40.3 ± 8.3) and compared at baseline to 22 healthy individuals. Serum levels of leptin, insulin, proinsulin and glucose were assessed at baseline and after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Leptin; Weight reduction; Dexamethasone.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400007

Leptospira interrogans activation of peripheral blood monocyte glycolipoprotein demonstrated in whole blood by the release of IL-6
Provedor de dados: 56
Autores: Dorigatti,F.; Brunialti,M.K.C.; Romero,E.C.; Kallas,E.G.; Salomão,R.
Glycolipoprotein (GLP) from pathogenic serovars of Leptospira has been implicated in the pathogenesis of leptospirosis by its presence in tissues of experimental animals with leptospirosis, the inhibition of the Na,K-ATPase pump activity, and induced production of cytokines. The aims of the present study were to investigate the induction of IL-6 by GLP in peripheral blood mononuclear cells (PBMC) and to demonstrate monocyte stimulation at the cellular level in whole blood from healthy volunteers. PBMC were stimulated with increasing concentrations (5 to 2500 ng/ml) of GLP extracted from the pathogenic L. interrogans serovar Copenhageni, lipopolysaccharide (positive control) or medium (negative control), and supernatants were collected after 6, 20/24, and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glycolipoprotein; Leptospira interrogans; Interleukin-6; Monocytes; CD45; CD14.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600013

Synaptic vesicle pool size, release probability and synaptic depression are sensitive to Ca2+ buffering capacity in the developing rat calyx of Held
Provedor de dados: 56
Autores: Leão,R.M.; von Gersdorff,H.
The calyx of Held, a specialized synaptic terminal in the medial nucleus of the trapezoid body, undergoes a series of changes during postnatal development that prepares this synapse for reliable high frequency firing. These changes reduce short-term synaptic depression during tetanic stimulation and thereby prevent action potential failures during a stimulus train. We measured presynaptic membrane capacitance changes in calyces from young postnatal day 5-7 (p5-7) or older (p10-12) rat pups to examine the effect of calcium buffer capacity on vesicle pool size and the efficiency of exocytosis. Vesicle pool size was sensitive to the choice and concentration of exogenous Ca2+ buffer, and this sensitivity was much stronger in younger animals. Pool size and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calyx of Held; Neurotransmission; Calcium buffer; Capacitance measurements; Mammalian auditory brainstem; Synaptic development.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100014

MicroRNA-451a, microRNA-34a-5p, and microRNA-221-3p as predictors of response to antidepressant treatment
Provedor de dados: 56
Autores: Kuang,Wei-Hong; Dong,Zai-Quan; Tian,Lian-Tian; Li,Jin.
Aberrant expression of microRNAs (miRNAs) has been shown to be involved in early observations of depression. The aim of this study was to determine if serum levels of miRNA-451a, miRNA-34a-5p, and miRNA-221-3p can serve as indicators of disease progression or therapeutic efficacy in depression. We collected data from 84 depressed patients and 78 control volunteers recruited from the medical staff at the West China Hospital. Depression severity was rated using the 24-item Hamilton Depression Scale (HAMD). Serum miRNA-451a, miRNA-34a-5p, and miRNA-221-3p levels were determined in samples from the depressed patients before and 8 weeks after antidepressant treatment as well as in samples from controls. Compared with the controls, the patients had lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNA-451a; MicroRNA-34a-5p; MicroRNA-221-3p; Depression; Hamilton Depression Scale; Paroxetine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700609

N-terminal prohormone brain natriuretic peptide (NT-proBNP) as a noninvasive marker for restrictive syndromes
Provedor de dados: 56
Autores: Mady,C.; Fernandes,F.; Ramires,F.J.A.; Nastari,L.; Buck,P.C.; Arteaga,E.; Ianni,B.M.; Salemi,V.M.C.
Constrictive pericarditis (CP) and restrictive cardiomyopathy share many similarities in both their clinical and hemodynamic characteristics and N-terminal prohormone brain natriuretic peptide (NT-proBNP) is a sensitive marker of cardiac diastolic dysfunction. The objectives of the present study were to determine whether serum NT-proBNP was high in patients with endomyocardial fibrosis (EMF) and CP, and to investigate how this relates to diastolic dysfunction. Thirty-three patients were divided into two groups: CP (16 patients) and EMF (17 patients). The control group consisted of 30 healthy individuals. Patients were evaluated by bidimensional echocardiography, with restriction syndrome evaluated by pulsed Doppler of the mitral flow and serum NT-proBNP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Constrictive pericarditis; Endomyocardial fibrosis; NT-proBNP; Restrictive cardiomyopathy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800004

Cell therapy in dilated cardiomyopathy: from animal models to clinical trials
Provedor de dados: 56
Autores: del Corsso,C.; Campos de Carvalho,A.C.
Dilated cardiomyopathy can be the end-stage form and common denominator of several cardiac disorders of known cause, such as hypertensive, ischemic, diabetic and Chagasic diseases. However, some individuals have clinical findings, such as an increase in ventricular chamber size and impaired contractility (classical manifestations of dilated cardiomyopathy) even in the absence of a diagnosed primary disease. In these patients, dilated cardiomyopathy is classified as idiopathic since its etiology is obscure. Nevertheless, regardless of all of the advances in medical, pharmacological and surgical procedures, the fate of patients with dilated cardiomyopathy (of idiopathic or of any other known cause) is linked to arrhythmic episodes, severe congestive heart...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dilated cardiomyopathy; Stem cell; Heart failure; Chagas’ disease; Cell therapy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500002

Lipopolysaccharide-induced dental pulp cell apoptosis and the expression of Bax and Bcl-2 in vitro
Provedor de dados: 56
Autores: Yang,H.; Zhu,Y.T.; Cheng,R.; Shao,M.Y.; Fu,Z.S.; Cheng,L.; Wang,F.M.; Hu,T.
Lipopolysaccharide exerts many effects on many cell lines, including cytokine secretion, and cell apoptosis and necrosis. We investigated the in vitro effects of lipopolysaccharide on apoptosis of cultured human dental pulp cells and the expression of Bcl-2 and Bax. Dental pulp cells showed morphologies typical of apoptosis after exposure to lipopolysaccharide. Flow cytometry showed that the rate of apoptosis of human dental pulp cells increased with increasing lipopolysaccharide concentration. Compared with controls, lipopolysaccharide promoted pulp cell apoptosis (P &lt; 0.05) from 0.1 to 100 μg/mL but not at 0.01 μg/mL. Cell apoptosis was statistically higher after exposure to lipopolysaccharide for 3 days compared with 1 day, but no difference was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dental pulp cells; Lipopolysaccharide; Apoptosis; Bax; Bcl-2.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100003

Participation of the endoplasmic reticulum protein chaperone thio-oxidoreductase in gonadotropin-releasing hormone receptor expression at the plasma membrane
Provedor de dados: 56
Autores: Lucca-Junior,W.; Janovick,J.A.; Conn,P.M.
Chaperone members of the protein disulfide isomerase family can catalyze the thiol-disulfide exchange reaction with pairs of cysteines. There are 14 protein disulfide isomerase family members, but the ability to catalyze a thiol disulfide exchange reaction has not been demonstrated for all of them. Human endoplasmic reticulum protein chaperone thio-oxidoreductase (ERp18) shows partial oxidative activity as a protein disulfide isomerase. The aim of the present study was to evaluate the participation of ERp18 in gonadotropin-releasing hormone receptor (GnRHR) expression at the plasma membrane. Cos-7 cells were cultured, plated, and transfected with 25 ng (unless indicated) wild-type human GnRHR (hGnRHR) or mutant GnRHR (Cys14Ala and Cys200Ala) and pcDNA3.1...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Protein chaperones; Gonadotropin-releasing hormone receptor; Calnexin; Calreticulin; ERp18.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200003

Circulating high mobility group box-1 and toll-like receptor 4 expressions increase the risk and severity of epilepsy
Provedor de dados: 56
Autores: Kan,Minchen; Song,Lihong; Zhang,Xueqiang; Zhang,Jing; Fang,Pingping.
This study aimed to investigate the association of serum high-mobility group box-1 (HMGB1) and toll-like receptor 4 (TLR4) expressions with the risk of epilepsy as well as their correlations with disease severity and resistance to anti-epilepsy drugs. One hundred and five epilepsy patients and 100 healthy controls (HCs) were enrolled in this case-control study, and serum samples were collected from all participants to assess the HMGB1 and TLR4 expressions by enzyme-linked immunosorbent assay (ELISA). Both serum HMGB1 (P&lt;0.001) and TLR4 (P&lt;0.001) expressions were higher in epilepsy patients than in HCs, and they displayed good predictive values for risk of epilepsy. Moreover, HMGB1 was positively correlated with TLR4 level (r=0.735, P&lt;0.001). HMGB1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High-mobility group box-1 (HMGB1); Toll-like receptor 4 (TLR4); Epilepsy; Severity; Drug resistance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700603

Mortality rate after open Roux-in-Y gastric bypass: a 10-year follow-up
Provedor de dados: 56
Autores: Bruschi Kelles,S.M.; Diniz,M.F.H.S.; Machado,C.J.; Barreto,S.M.
The prevalence of obesity has increased to epidemic status worldwide. Thousands of morbidly obese individuals undergo bariatric surgery for sustained weight loss; however, mid- and long-term outcomes of this surgery are still uncertain. Our objective was to estimate the 10-year mortality rate, and determine risk factors associated with death in young morbidly obese adults who underwent bariatric surgery. All patients who underwent open Roux-in-Y gastric bypass surgery between 2001 and 2010, covered by an insurance company, were analyzed to determine possible associations between risk factors present at the time of surgery and deaths related and unrelated to the surgery. Among the 4344 patients included in the study, 79% were female with a median age of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Survival analysis; Mortality; Follow-up studies; Morbid obesity; Roux-in-Y gastric bypass; Surgeon expertise.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700617

Clonal dissemination of VanA-type glycopeptide-resistant Enterococcus faecalis between hospitals of two cities located 100 km apart
Provedor de dados: 56
Autores: Moretti,M.L.; Bratfich,O.J.; Stucchi,R.B.; Levi,C.; Levin,A.S.; Duboc,G.M.; Vormittag,E.; Blum-Menezes,D.
Nosocomial dissemination of glycopeptide-resistant enterococci represents a major problem in hospitals worldwide. In Brazil, the dissemination among hospitals in the city of São Paulo of polyclonal DNA profiles was previously described for vancomycin-resistant Enterococcus faecium. We describe here the dissemination of VanA phenotype E. faecalis between two hospitals located in different cities in the State of São Paulo. The index outbreak occurred in a tertiary care university hospital (HCUSP) in the city of São Paulo and three years later a cluster caused by the same strain was recognized in two patients hospitalized in a private tertiary care hospital (CMC) located 100 km away in the interior of the state. From May to July 1999, 10 strains of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Vancomycin-resistant Enterococcus; Enterococcus faecalis; Tn1546; Nosocomial transmission; São Paulo.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900008

Effects of tryptophan depletion on anxiety induced by simulated public speaking
Provedor de dados: 56
Autores: Monteiro-dos-Santos,P.C.; Graeff,F.G.; dos-Santos,J.E.; Ribeiro,R.P.P.; Guimarães,F.S.; Zuardi,A.W.
Several lines of evidence point to the participation of serotonin (5HT) in anxiety. Its specific role, however, remains obscure. The objective of the present study was to evaluate the effect of reducing 5HT-neurotransmission through an acute tryptophan depletion on anxiety induced by a simulated public speaking (SPS) test. Two groups of 14-15 subjects were submitted to a 24-h diet with a low or normal content of tryptophan and received an amino acid mixture without (TRY-) or with (TRY+) tryptophan under double-blind conditions. Five hours later they were submitted to the SPS test. The state-trait anxiety inventory (STAI) and the visual analogue mood scale (VAMS) were used to measure subjective anxiety. Both scales showed that SPS induced a significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serotonin; Anxiety; Tryptophan depletion; Public speaking; Healthy volunteers; Gender differences.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500013

A detailed description of an economical setup for electroporation of chick embryos in ovo
Provedor de dados: 56
Autores: Borges,R.M.; Horne,J.H.; Melo,A.; Vidal,J.T.; Vieceli,F.M.; Melo,M.O.; Kanno,T.Y.N.; Fraser,S.E.; Yan,C.Y.I.
One of the challenges of the postgenomic era is characterizing the function and regulation of specific genes. For various reasons, the early chick embryo can easily be adopted as an in vivo assay of gene function and regulation. The embryos are robust, accessible, easily manipulated, and maintained in the laboratory. Genomic resources centered on vertebrate organisms increase daily. As a consequence of optimization of gene transfer protocols by electroporation, the chick embryo will probably become increasingly popular for reverse genetic analysis. The challenge of establishing chick embryonic electroporation might seem insurmountable to those who are unfamiliar with experimental embryological methods. To minimize the cost, time, and effort required to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electroporation; In vivo gene expression; Electroporator; Vertebrate embryo.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900752

Changes in the TBARs content and superoxide dismutase, catalase and glutathione peroxidase activities in the lymphoid organs and skeletal muscles of adrenodemedullated rats
Provedor de dados: 56
Autores: Pereira,B.; Costa-Rosa,L.F.B.P.; Bechara,E.J.H.; Newsholme,P.; Curi,R.
Thiobarbituric acid reactant substances (TBARs) content, and the activities of glucose-6-phosphate dehydrogenase (G6PDh), citrate synthase (CS), Cu/Zn- and Mn-superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPX) were measured in the lymphoid organs (thymus, spleen, and mesenteric lymph nodes (MLN)) and skeletal muscles (gastrocnemius and soleus) of adrenodemedullated (ADM) rats. The results were compared with those obtained for sham-operated rats. TBARs content was reduced by adrenodemedullation in the lymphoid organs (MLN (28%), thymus (40%) and spleen (42%)) and gastrocnemius muscle (67%). G6PDh activity was enhanced in the MLN (69%) and reduced in the spleen (28%) and soleus muscle (75%). CS activity was reduced in all tissues (MLN...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epinephrine; Lymphoid organs; Superoxide dismutase; Catalase; Glutathione peroxidase; TBARs.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600016

Evidence for the expression of native Mycobacterium tuberculosis phospholipase C: recognition by immune sera and detection of promoter activity
Provedor de dados: 56
Autores: Matsui,T.; Carneiro,C.R.W.; Leão,S.C.
The genome of Mycobacterium tuberculosis H37Rv contains three contiguous genes (plc-a, plc-b and plc-c) which are similar to the Pseudomonas aeruginosa phospholipase C (PLC) genes. Expression of mycobacterial PLC-a and PLC-b in E. coli and M. smegmatis has been reported, whereas expression of the native proteins in M. tuberculosis H37Rv has not been demonstrated. The objective of the present study was to demonstrate that native PLC-a is expressed in M. tuberculosis H37Rv. Sera from mice immunized with recombinant PLC-a expressed in E. coli were used in immunoblots to evaluate PLC-a expression. The immune serum recognized a 49-kDa protein in immunoblots against M. tuberculosis extracts. No bands were visible in M. tuberculosis culture supernatants or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Phospholipase C; Gene expression; Promoters.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100003

Involvement of midbrain tectum neurokinin-mediated mechanisms in fear and anxiety
Provedor de dados: 56
Autores: Brenes,J.C.; Broiz,A.C.; Bassi,G.S.; Schwarting,R.K.W.; Brandão,M.L.
Electrical stimulation of midbrain tectum structures, particularly the dorsal periaqueductal gray (dPAG) and inferior colliculus (IC), produces defensive responses, such as freezing and escape behavior. Freezing also ensues after termination of dPAG stimulation (post-stimulation freezing). These defensive reaction responses are critically mediated by γ-aminobutyric acid and 5-hydroxytryptamine mechanisms in the midbrain tectum. Neurokinins (NKs) also play a role in the mediation of dPAG stimulation-evoked fear, but how NK receptors are involved in the global processing and expression of fear at the level of the midbrain tectum is yet unclear. The present study investigated the role of NK-1 receptors in unconditioned defensive behavior induced by electrical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurokinin-1 receptors; Dorsal periaqueductal gray; Spantide; Unconditioned fear; Elevated plus maze; Ultrasonic vocalizations.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400009

Multifactorial control of water and saline intake: role of a2-adrenoceptors
Provedor de dados: 56
Water and saline intake is controlled by several mechanisms activated during dehydration. Some mechanisms, such as the production of angiotensin II and unloading of cardiovascular receptors, activate both behaviors, while others, such as the increase in blood osmolality or sodium concentration, activate water, but inhibit saline intake. Aldosterone probably activates only saline intake. Clonidine, an &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;2-adrenergic agonist, inhibits water and saline intake induced by these mechanisms. One model to describe the interactions between these multiple mechanisms is a wire-block diagram, where the brain circuit that controls each intake is represented by a summing point of its respective inhibiting and activating factors. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sodium intake water intake clonidine noradrenaline dehydration &lt; FONT FACE=Symbol&gt; A&lt; /font&gt; 2-adrenergic agonist.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400011

Double-blind trial of the efficacy of pentoxifylline vs thalidomide for the treatment of type II reaction in leprosy
Provedor de dados: 56
Autores: Sales,A.M.; Matos,H.J.de; Nery,J.A.C.; Duppre,N.C.; Sampaio,E.P.; Sarno,E.N.
Type II reaction in leprosy, or erythema nodosum leprosum (ENL), is often characterized by severe clinical symptoms together with nerve function impairment leading to permanent disabilities. Thalidomide has been shown to be a highly effective drug for the treatment of ENL. It is, however, contraindicated for women of childbearing age due to its teratogenicity. On the other hand, pentoxifylline, used to treat hypercoagulable states, is not teratogenic and, like thalidomide, can inhibit the synthesis of tumor necrosis factor-a and other cytokines. In the present randomized double-blind clinical study we compared the effectiveness of orally administered pentoxifylline vs thalidomide in treating type II reaction in 44 patients. Daily doses of 300 mg...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leprosy; Reactional episodes; Therapy; Pentoxifylline; Thalidomide.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200011

Roles of mitogen-activated protein kinases and angiotensin II in renal development
Provedor de dados: 56
Autores: Balbi,A.P.C.; Francescato,H.D.C.; Marin,E.C.S.; Costa,R.S.; Coimbra,T.M.
Experimental and clinical evidence suggests that angiotensin II (AII) participates in renal development. Renal AII content is several-fold higher in newborn rats and mice than in adult animals. AII receptors are also expressed in higher amounts in the kidneys of newborn rats. The kidneys of fetuses whose mother received a type 1 AII receptor (AT1) antagonist during gestation present several morphological alterations. Mutations in genes that encode components of the renin-angiotensin system are associated with autosomal recessive renal tubular dysgenesis. Morphological changes were detected in the kidneys of 3-week-old angiotensin-deficient mice. Mitogen-activated protein kinases (MAPKs) are important mediators that transduce extracellular stimuli to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal development; Angiotensin II; Mitogen-activated protein kinase; Angiotensin II antagonists; Sodium intake.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100007

Invasive pulmonary fungal infections in patients with connective tissue disease: a retrospective study from northern China
Provedor de dados: 56
Autores: Ge,H.F.; Liu,X.Q.; Zhu,Y.Q.; Chen,H.Q.; Chen,G.Z.
Invasive pulmonary fungal infection (IPFI) is a potentially fatal complication in patients with connective tissue disease (CTD). The current study aimed to uncover the clinical characteristics and risk factors of patients with IPFI-CTD. The files of 2186 CTD patients admitted to a single center in northern China between January 2011 and December 2013 were retrospectively reviewed. A total of 47 CTD patients with IPFI were enrolled into this study and assigned to the CTD-IPFI group, while 47 uninfected CTD patients were assigned to the control group. Clinical manifestations were recorded, and risk factors of IPFI were calculated by stepwise logistical regression analysis. Forty-seven (2.15%) CTD patients developed IPFI. Systemic lupus erythematosus patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Connective tissue diseases; Invasive pulmonary fungal infection; Pulmonary disease; Retrospective study.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000705

High doses of riboflavin and the elimination of dietary red meat promote the recovery of some motor functions in Parkinson's disease patients
Provedor de dados: 56
Autores: Coimbra,C.G.; Junqueira,V.B.C.
Abnormal riboflavin status in the absence of a dietary deficiency was detected in 31 consecutive outpatients with Parkinson's disease (PD), while the classical determinants of homocysteine levels (B6, folic acid, and B12) were usually within normal limits. In contrast, only 3 of 10 consecutive outpatients with dementia without previous stroke had abnormal riboflavin status. The data for 12 patients who did not complete 6 months of therapy or did not comply with the proposed treatment paradigm were excluded from analysis. Nineteen PD patients (8 males and 11 females, mean age ± SD = 66.2 ± 8.6 years; 3, 3, 2, 5, and 6 patients in Hoehn and Yahr stages I to V) received riboflavin orally (30 mg every 8 h) plus their usual symptomatic medications and all red...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parkinson's disease; Riboflavin; Flavin-adenine dinucleotide; Glutathione; Iron; Hemin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000019

Effect of prostaglandin A1 in the induction of stress proteins in Aedes albopictus cells
Provedor de dados: 56
Autores: Barbosa,J.A.; Rebello,M.A.
Prostaglandins are natural fatty acid derivatives with diverse physiological effects, including immune function and the control of cell growth. While the action of prostaglandins in the induction of stress proteins in vertebrate cells is well documented, their functions in invertebrate cells have been poorly investigated. The purpose of the present study was to investigate the effect of prostaglandin A1 (PGA1; 0.25, 1.25 and 12.5 µg/ml) on protein synthesis during the growth of Aedes albopictus cells. We found that PGA1 stimulates the synthesis of several polypeptides with molecular masses of 87, 80, 70, 57, 29, 27 and 23 kDa in Aedes albopictus cells. When the proteins induced by PGA1 and those induced by heat treatment were compared by polyacrylamide gel...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Prostaglandin; Stress proteins; Aedes albopictus cells.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400004

Patients with Duchenne and Becker muscular dystrophies are not more asymmetrical than healthy controls on timed performance of upper limb tasks
Provedor de dados: 56
Autores: Artilheiro,M.C.; Sá,C.S.C.; Fávero,F.M.; Caromano,F.A.; Voos,M.C.
This study aimed to investigate possible asymmetries and relationships between performance of dominant and non-dominant upper limbs (UL) in patients with Duchenne and Becker muscular dystrophies (DMD/BMD), to compare UL performance of patients and healthy subjects and to investigate the relationship between timed performance of UL and age, motor function and muscle strength in DMD/BMD patients. Sixteen patients with DMD and 3 with BMD were evaluated with Jebsen-Taylor Test (timed performance), Vignos scale and Dimension 3 of Motor Function Measure (motor function), and Medical Research Council scale (muscle strength) on a single session. ANOVA showed no asymmetry between dominant and non-dominant UL, except in the writing subtest, in patients and in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Duchenne muscular dystrophy; Upper extremity; Evaluation; Physical therapy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800604

Antiprotozoal and molluscicidal activities of five Brazilian plants
Provedor de dados: 56
Autores: Truiti,M.C.T.; Ferreira,I.C.P.; Zamuner,M.L.M.; Nakamura,C.V.; Sarragiotto,M.H.; Souza,M.C.
Leishmaniasis, Chagas' disease and schistosomiasis (bilharzia) are parasitic diseases with wide distribution on the American continent, affecting millions of people. In the present study, biological assays for antiprotozoal and molluscicidal activities were carried out with ethanolic extracts of plant species from the Brazilian part of the Upper Paraná River. Crude extracts were obtained by percolation with absolute ethanol from the leaves of Cayaponia podantha Cogn., Nectandra falcifolia (Nees) Castiglioni and Paullinia elegans Cambess., as well as from the aerial parts of Helicteres gardneriana St. Hil. &amp;amp; Naud. and Melochia arenosa Benth., all belonging to genera used in folk medicine. Trypanocidal activity of plants was assayed on epimastigote...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cayaponia podantha; Helicteres gardneriana; Melochia arenosa; Nectandra falcifolia; Paullinia elegans; Antiprotozoal activity; Molluscicidal activity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200016

Association between type of feeding at discharge from the hospital and nutritional status of very low birth weight preterm infants
Provedor de dados: 56
Autores: Martins-Celini,F.P.; Gonçalves-Ferri,W.A.; Aragon,D.C.; Bernichi,J.P.; Calixto,C.; Sacramento,E.M.F.; Santos,M.A.; Martinez,F.E.
The ideal feeding for premature babies has been the source of extensive debate. The aim of this study was to assess the association between type of feeding at discharge and the nutritional status of very low birth weight infants. This was a retrospective cohort of preterm babies with birth weight ≤1500 g, born between January 2006 and December 2013. The infants were divided into 3 groups according to type of feeding at discharge: exclusive breast milk (group 1), mixed feeding (group 2) and exclusive artificial formula (group 3). Frequencies of each group were calculated, as well as mean Z-score differences in weight, length and head circumference. Six hundred and forty-nine newborns were included. The mean weight of groups 1, 2, and 3 was 1338.7, 1104.0,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Very low birth weight; Nutrition; Feeding; Nutritional status; Human milk.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300614

The effect of sun exposure on 25-hydroxyvitamin D concentrations in young healthy subjects living in the city of São Paulo, Brazil
Provedor de dados: 56
Autores: Maeda,S.S.; Kunii,I.S.; Hayashi,L.; Lazaretti-Castro,M.
The range of 25-hydroxyvitamin D (25OHD) concentration was determined in a young healthy population based on bone metabolism parameters and environmental and behavioral aspects. We studied 121 healthy young volunteers (49 men, 72 women) living in São Paulo (23º 34' south latitude) belonging to three occupational categories: indoor workers (N = 28), medical school students (N = 44), and resident physicians (N = 49). Fasting morning blood samples were collected once from each volunteer from August 2002 to February 2004, and 25OHD, total calcium, albumin, alkaline phosphatase, phosphorus, creatinine, intact parathyroid hormone, osteocalcin, and type I collagen carboxyterminal telopeptide were measured. Data are reported as means ± SD. Mean subject age was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 25-Hydroxyvitamin D; Parathyroid hormone; Occupational aspects; Seasonal influence; 25OHD and solar exposure.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200009

Genetic analysis of the ELOVL6 gene polymorphism associated with type 2 diabetes mellitus
Provedor de dados: 56
Autores: Liu,Y.; Wang,F.; Yu,X.L.; Miao,Z.M.; Wang,Z.C.; Chen,Y.; Wang,Y.G.
Recent animal studies have indicated that overexpression of the elongation of long-chain fatty acids family member 6 (Elovl6) gene can cause insulin resistance and β-cell dysfunction. These are the major factors involved in the development of type 2 diabetes mellitus (T2DM). To identify the relationship between single nucleotide polymorphisms (SNP) ofELOVL6 and T2DM pathogenesis, we conducted a case-control study of 610 Han Chinese individuals (328 newly diagnosed T2DM and 282 healthy subjects). Insulin resistance and islet first-phase secretion function were evaluated by assessment of insulin resistance in a homeostasis model (HOMA-IR) and an arginine stimulation test. Three SNPs of the ELOVL6 gene were genotyped with polymerase chain reaction-restriction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elongation of long-chain fatty acids family member 6; Single nucleotide polymorphism; Type 2 diabetes mellitus; Insulin resistance; Β-cell function.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700623

Serotonin regulates osteoblast proliferation and function in vitro
Provedor de dados: 56
Autores: Dai,S.Q.; Yu,L.P.; Shi,X.; Wu,H.; Shao,P.; Yin,G.Y.; Wei,Y.Z.
The monoamine serotonin (5-hydroxytryptamine, 5-HT), a well-known neurotransmitter, also has important functions outside the central nervous system. The objective of this study was to investigate the role of 5-HT in the proliferation, differentiation, and function of osteoblasts in vitro. We treated rat primary calvarial osteoblasts with various concentrations of 5-HT (1 nM to 10 µM) and assessed the rate of osteoblast proliferation, expression levels of osteoblast-specific proteins and genes, and the ability to form mineralized nodules. Next, we detected which 5-HT receptor subtypes were expressed in rat osteoblasts at different stages of osteoblast differentiation. We found that 5-HT could inhibit osteoblast proliferation, differentiation, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serotonin; Osteoblast; Proliferation; Differentiation; 5-HT receptors; 5-HT.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900759

Repeated exposure of adolescent rats to oral methylphenidate does not induce behavioral sensitization or cross-sensitization to nicotine
Provedor de dados: 56
Autores: Justo,C.C.; Carneiro-de-Oliveira,P.E.; DeLucia,R.; Aizenstein,M.L.; Planeta,C.S.
Several lines of evidence indicate that the use of stimulant drugs, including methylphenidate (MPD), increases tobacco smoking. This has raised concerns that MPD use during adolescence could facilitate nicotine abuse. Preclinical studies have shown that repeated treatment with an addictive drug produces sensitization to that drug and usually cross-sensitization to other drugs. Behavioral sensitization has been implicated in the development of drug addiction. We examined whether repeated oral MPD administration during adolescence could induce behavioral sensitization to MPD and long-lasting cross-sensitization to nicotine. Adolescent male Wistar rats were treated orally with 10 mg/kg MPD or saline (SAL) from postnatal day (PND) 27 to 33. To evaluate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylphenidate; Nicotine; Behavioral sensitization; Adolescent rats.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700007

Two pedigrees with restless legs syndrome in Brazil
Provedor de dados: 56
Autores: Esteves,A.M.; Pedrazzoli,M.; Bagnato,M.; Moreira,F.; Mello,M.T. de; Tufik,S.
Numerous studies have suggested a substantial genetic contribution in the etiology of the primary form of restless legs syndrome (RLS) and periodic leg movements (PLM). We describe the symptoms, the sleep profiles and physiological parameters of two families in which several members present RLS/PLM. The proband of family 1 is a 70-year-old woman and the proband of family 2 is a 57-year-old woman; both have exhibited the symptoms since the age of 20 years. All patients in both families were diagnosed with RLS according to the criteria of the International RLS Study Group. Polysomnographic recordings were performed to quantify and to describe PLM during sleep. Sleep parameters showed decreased sleep efficiency, increased sleep latency in the arousal index...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Restless legs syndrome; Periodic leg movements; Genetics; Sleep.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200005

New strategies in hematopoietic stem cell transplantation: G-CSF-mobilized unprocessed whole blood
Provedor de dados: 56
Autores: Dräger,A.M.; Ossenkoppele,G.J.; Jonkhoff,A.R.; Schuurhuis,G.J.; Huijgens,P.C.
Transplantation of mobilized peripheral blood stem cells (PBSC) for rescue of bone marrow function after high-dose chemo-/radiotherapy is widely used in hematologic malignancies and solid tumors. Mobilization of stem cells to the peripheral blood can be achieved by cytokine treatment of the patients. The main advantage of autologous PBSC transplantation over bone marrow transplantation is the faster recovery of neutrophil and platelet counts. The threshold number of PBSC required for adequate rescue of bone marrow is thought to be about 2 x 106 CD34+ cells/kg, if the stem cells are collected by leukapheresis and subsequently cryopreserved. We show that this critical number could be further reduced to as few as 0.2 x 106 cells/kg. In 30 patients with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematopoietic stem cells; Transplantation; Cytokines; G-CSF; Lymphoma; Multiple myeloma.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100006

Mutations of androgen receptor gene in Brazilian patients with male pseudohermaphroditism
Provedor de dados: 56
Autores: Cabral,D.F.; Maciel-Guerra,A.T.; Hackel,C.
We describe the identification of point mutations in the androgen receptor gene in five Brazilian patients with female assignment and behavior. The eight exons of the gene were amplified by the polymerase chain reaction (PCR) and analyzed for single-strand conformation polymorphism (SSCP) to detect the mutations. Direct sequencing of the mutant PCR products demonstrated single transitions in three of these cases: G&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;A in case 1, within exon C, changing codon 615 from Arg to His; G&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;A in case 2, within exon E, changing codon 752 from Arg to Gln, and C&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;T in case 3, within exon B, but without amino acid change.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Androgen receptor gene; Gene mutations; Androgen insensitivity syndromes; Male pseudohermaphroditism.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600008

Acute effect of amiodarone on cardiovascular reflexes of normotensive and renal hypertensive rats
Provedor de dados: 56
Autores: Oliveira,P.F.; Dias da Silva,V.J.; Salgado,M.C.O.; Fazan Jr.,R.; Aguiar,C.A.; Salgado,H.C.
The aim of the present study was to evaluate the effect of amiodarone on mean arterial pressure (MAP), heart rate (HR), baroreflex, Bezold-Jarisch, and peripheral chemoreflex in normotensive and chronic one-kidney, one-clip (1K1C) hypertensive rats (N = 9 to 11 rats in each group). Amiodarone (50 mg/kg, iv) elicited hypotension and bradycardia in normotensive (-10 ± 1 mmHg, -57 ± 6 bpm) and hypertensive rats (-37 ± 7 mmHg, -39 ± 19 bpm). The baroreflex index (deltaHR/deltaMAP) was significantly attenuated by amiodarone in both normotensive (-0.61 ± 0.12 vs -1.47 ± 0.14 bpm/mmHg for reflex bradycardia and -1.15 ± 0.19 vs -2.63 ± 0.26 bpm/mmHg for reflex tachycardia) and hypertensive rats (-0.26 ± 0.05 vs -0.72 ± 0.16 bpm/mmHg for reflex bradycardia and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amiodarone; Baroreflex; Bezold-Jarisch reflex; Chemoreflex; Autonomic nervous system; 1K1C hypertension.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600020

Topical application of a melanotropin analogue to vulgar vitiligo dermo-epidermal minigrafts
Provedor de dados: 56
Autores: Schwartzmann-Solon,A.M.; Visconti,M.A.; Castrucci,A.M.L.
Human subjects with active vulgar vitiligo do not respond well to autologous dermo-epidermal minigrafting. Eighteen subjects were treated with the &lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;-melanocyte-stimulating hormone (&lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;-MSH) synthetic analogue &amp;amp;#091;Nle4, D-Phe7&amp;amp;#093;-&lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;-MSH. The hormone (50 µl, 0.4 mM) was applied topically to 30-cm2 lesions in which 29-48 minigrafts had been made. The hormone did not improve the success of the minigrafting and no differences were observed in local or distant repigmentation in treated subjects as compared to the placebo group. Aliquots of 24-h urine concentrated by lyophilization irreversibly darkened toad skins, demonstrating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vulgar vitiligo &lt; FONT FACE=Symbol&gt; A&lt; /FONT&gt; -MSH synthetic analogue minigrafting.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200008

Chronic intrathecal cannulation enhances nociceptive responses in rats
Provedor de dados: 56
Autores: Almeida,F.R.C.; Schivo,I.R.S.; Lorenzetti,B.B.; Ferreira,S.H.
The influence of a chronically implanted spinal cannula on the nociceptive response induced by mechanical, chemical or thermal stimuli was evaluated. The hyperalgesia in response to mechanical stimulation induced by carrageenin or prostaglandin E2 (PGE2) was significantly increased in cannulated (Cn) rats, compared with naive (Nv) or sham-operated (Sh) rats. Only Cn animals presented an enhanced nociceptive response in the first phase of the formalin test when low doses were used (0.3 and 1%). The withdrawal latency to thermal stimulation of a paw inflamed by carrageenin was significantly reduced in Cn rats but not in Nv or Sh rats. In contrast to Nv and Sh rats, injection in Cn animals of a standard non-steroid anti-inflammatory drug, indomethacin, either...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperalgesia; Spinal sensitization; Intrathecal cannulation; Nociceptive response; Inflammation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800011

CCR3 antagonist impairs estradiol-induced eosinophil migration to the uterus in ovariectomized mice
Provedor de dados: 56
Autores: Araújo,J.M.D.; Silva,L.A.S.; Felix,F.B.; Camargo,E.A.; Grespan,R.
Eosinophils are abundant in the reproductive tract, contributing to the remodeling and successful implantation of the embryo. However, the mechanisms by which eosinophils migrate into the uterus and their relationship to edema are still not entirely clear, since there are a variety of chemotactic factors that can cause migration of these cells. Therefore, to evaluate the role of CCR3 in eosinophil migration, ovariectomized C57BL/6 mice were treated with CCR3 antagonist SB 328437 and 17β-estradiol. The hypothesis that the CCR3 receptor plays an important role in eosinophil migration to the mouse uterus was confirmed, because we observed reduction in eosinophil peroxidase activity in these antagonist-treated uteruses. The antagonist also influenced uterine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estradiol; Chemokine receptor; Immunology; Menopause; Uterus.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100603

The use of protein structure/activity relationships in the rational design of stable particulate delivery systems
Provedor de dados: 56
Autores: Costa,M.H.B.; Quintilio,W.; Sant'Anna,O.A.; Faljoni-Alário,A.; Araujo,P.S. de.
The recombinant heat shock protein (18 kDa-hsp) from Mycobacterium leprae was studied as a T-epitope model for vaccine development. We present a structural analysis of the stability of recombinant 18 kDa-hsp during different processing steps. Circular dichroism and ELISA were used to monitor protein structure after thermal stress, lyophilization and chemical modification. We observed that the 18 kDa-hsp is extremely resistant to a wide range of temperatures (60% of activity is retained at 80ºC for 20 min). N-Acylation increased its ordered structure by 4% and decreased its ß-T1 structure by 2%. ELISA demonstrated that the native conformation of the 18 kDa-hsp was preserved after hydrophobic modification by acylation. The recombinant 18 kDa-hsp resists to a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Protein stability; Hydrophobic modification; Vaccine delivery system; Drug delivery system; Adjuvant.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600014

Genetic correlation between Prothrombin G20210A polymorphism and retinal vein occlusion risk
Provedor de dados: 56
Autores: Zou,Yuanyuan; Zhang,Xi; Zhang,Jingyi; Ji,Xiangning; Liu,Yuqing; Zhao,Shaozhen.
The aim of this study was to perform an updated meta-analysis to quantitatively investigate the association between G20210A polymorphism of Prothrombin gene and the risk of retinal vein occlusion (RVO), based on the available publications with inconsistent results. We utilized the Stata software to perform the heterogeneity test, association test, Begg's and Egger's tests, and sensitivity analysis. We searched three on-line databases (PubMed, Embase, and WOS) and obtained a total of 422 articles. Based on our selection criteria, 24 case-control studies were finally enrolled in this overall meta-analysis; a subgroup analysis by the factors ethnicity, control source, and RVO type was done. Through the association test of overall meta-analysis, we did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prothrombin; Retinal vein occlusion; Polymorphism; Risk; Meta-analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400604

Air pollution and cardiovascular hospital admissions in a medium-sized city in São Paulo State, Brazil
Provedor de dados: 56
Autores: Nascimento,L.F.C.
The objective of the present study was to estimate the contribution of environmental pollutants to hospital admissions for cardiovascular disease. A time series ecological study was conducted on subjects aged over 60 years and living in São José dos Campos, Brazil, with a population near 700,000 inhabitants. Hospital admission data of public health patients (SUS) were obtained from DATASUS for the period between January 1, 2004 and December 31, 2006, according to the ICD-10 diagnoses I20 to I22 and I24. Particulate matter with less than 10 µm in aerodynamic diameter, sulfur dioxide and ozone were the pollutants examined, and the control variables were mean temperature and relative humidity. Data on pollutants were obtained from the São Paulo State Sanitary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Air pollution; Particulate matter; Cardiovascular diseases; Time series studies.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700017

Absence-like seizures in adult rats following pilocarpine-induced status epilepticus early in life
Provedor de dados: 56
Autores: Ferreira,B.L.C.; Valle,A.C.; Cavalheiro,E.A.; Timo-Iaria,C.
Administration of pilocarpine causes epilepsy in rats if status epilepticus (SE) is induced at an early age. To determine in detail the electrophysiological patterns of the epileptogenic activity in these animals, 46 Wistar rats, 7-17 days old, were subjected to SE induced by pilocarpine and electro-oscillograms from the cortex, hippocampus, amygdala, thalamus and hypothalamus, as well as head, rostrum and vibrissa, eye, ear and forelimb movements, were recorded 120 days later. Six control animals of the same age range did not show any signs of epilepsy. In all the rats subjected to SE, iterative spike-wave complexes (8.1 ± 0.5 Hz in frequency, 18.9 ± 9.1 s in duration) were recorded from the frontal cortex during absence fits. However, similar spike-wave...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epilepsy; Absence seizures; Spike-wave; Status epilepticus; Pilocarpine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200010

Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens
Provedor de dados: 56
Autores: Carbonell,G.V.; Alfieri,A.F.; Alfieri,A.A.; Vidotto,M.C.; Levy,C.E.; Darini,A.L.C.; Yanaguita,R.M.
Cytotoxin production was studied in 60 Serratia marcescens strains isolated from hospitalized patients. Association of cytotoxic activity with serotype, source of isolation and presence of plasmids was also evaluated. Thirteen of the 60 S. marcescens strains produced a cytotoxic effect on Vero cells. These strains were isolated from distinct clinical sources and classified into seven different serotypes (O1:H7; O4:NM; O10:NT; O19:NM; O6,14:H4; O6,14:NM and O6,14:H1). No relationship was observed between cytotoxic activity and clinical source or serotypes of the strains. Plasmids from five cytotoxin-producing S. marcescens strains were transferred to E. coli K12/711. The transconjugants did not exhibit cytotoxicity, indicating that the cytotoxic effect is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxin production; Nosocomial infections; Plasmids; Serratia marcescens; Virulence factors.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100005

Segmental resection combined with anoplasty for the treatment of circumferential mixed hemorrhoids
Provedor de dados: 56
Autores: Wu,Jing; Yu,Keqiang; Lv,Changyao; Lu,Wenzhu; He,Hongbo.
Circumferential mixed hemorrhoids are very difficult to treat non-surgically. Therefore, it is important to explore the surgical methods for its complete resolution as well as maintenance of normal anal anatomy and function. The present study was designed to evaluate the effect of segmented and plastic hemorrhoidectomy (SPH) on patients with circumferential mixed hemorrhoids. A total of 300 patients with circumferential mixed hemorrhoids were divided into experimental group (n=150) undergoing SPH and control group (n=150) undergoing Milligan-Morgan hemorrhoidectomy. There were no differences in cure and effectiveness rates between two groups. Compared with the control group, patients in the experimental group had shorter healing time (15.7±1.3 vs 12.5±0.7...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circumferential mixed hemorrhoids; Segmented and plastic hemorrhoidectomy; Anal function; Anal smoothness; Complications.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500610

Comparison of the Polar S810i monitor and the ECG for the analysis of heart rate variability in the time and frequency domains
Provedor de dados: 56
Autores: Vanderlei,L.C.M.; Silva,R.A.; Pastre,C.M.; Azevedo,F.M.; Godoy,M.F.
The aim of the present study was to compare heart rate variability (HRV) at rest and during exercise using a temporal series obtained with the Polar S810i monitor and a signal from a LYNX® signal conditioner (BIO EMG 1000 model) with a channel configured for the acquisition of ECG signals. Fifteen healthy subjects aged 20.9 ± 1.4 years were analyzed. The subjects remained at rest for 20 min and performed exercise for another 20 min with the workload selected to achieve 60% of submaximal heart rate. RR series were obtained for each individual with a Polar S810i instrument and with an ECG analyzed with a biological signal conditioner. The HRV indices (rMSSD, pNN50, LFnu, HFnu, and LF/HF) were calculated after signal processing and analysis. The unpaired...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart rate variability; Autonomic nervous system; Exercise; Spectral analysis; ECG; Polar S810i monitor.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000004

Investigating ego modules and pathways in osteosarcoma by integrating the EgoNet algorithm and pathway analysis
Provedor de dados: 56
Autores: Chen,X.Y.; Chen,Y.H.; Zhang,L.J.; Wang,Y.; Tong,Z.C.
Osteosarcoma (OS) is the most common primary bone malignancy, but current therapies are far from effective for all patients. A better understanding of the pathological mechanism of OS may help to achieve new treatments for this tumor. Hence, the objective of this study was to investigate ego modules and pathways in OS utilizing EgoNet algorithm and pathway-related analysis, and reveal pathological mechanisms underlying OS. The EgoNet algorithm comprises four steps: constructing background protein-protein interaction (PPI) network (PPIN) based on gene expression data and PPI data; extracting differential expression network (DEN) from the background PPIN; identifying ego genes according to topological features of genes in reweighted DEN; and collecting ego...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteosarcoma; Ego; Genes; Modules; Pathways.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200607

Use of a cysteine proteinase from Carica candamarcensis as a protective agent during DNA extraction
Provedor de dados: 56
Autores: Genelhu,M.S.; Zanini,M.S.; Veloso,I.F.; Carneiro,A.M.D.; Lopes,M.T.P.; Salas,C.E.
We describe the use of a plant cysteine proteinase isolated from latex of Carica candamarcensis as a protective agent during isolation of bacterial DNA following growth in culture of these cells. Between 100 to 720 units of proteinase (1 µg = 6 units) afforded good DNA protection when incubated with various kinds of microorganisms. Agarose gel electrophoresis showed that the resulting DNA was similar in size to DNA preparations obtained by treatment with proteinase K. The viability of the resulting material was checked by PCR amplification using species-specific primers. After standing at room temperature (25oC) for 35 days, the enzyme lost 10% of its initial activity. The enzyme stability and good yield of DNA suggest the use of this proteinase as an...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cysteine proteinase; Proteinase K; C. candamarcensis; DNA extraction.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900005

Age effects on the pharmacokinetics of tityustoxin from Tityus serrulatus scorpion venom in rats
Provedor de dados: 56
Autores: Nunan,E.A.; Arya,V.; Hochhaus,G.; Cardoso,V.N.; Moraes-Santos,T.
The pharmacokinetics of scorpion venom and its toxins has been investigated in experimental models using adult animals, although, severe scorpion accidents are associated more frequently with children. We compared the effect of age on the pharmacokinetics of tityustoxin, one of the most active principles of Tityus serrulatus venom, in young male/female rats (21-22 days old, N = 5-8) and in adult male rats (150-160 days old, N = 5-8). Tityustoxin (6 µg) labeled with 99mTechnetium was administered subcutaneously to young and adult rats. The plasma concentration vs time data were subjected to non-compartmental pharmacokinetic analysis to obtain estimates of various pharmacokinetic parameters such as total body clearance (CL/F), distribution volume (Vd/F),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venom; Tityustoxin; Pharmacokinetics; Age; Bootstrapping resampling; Non-compartmental analysis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300016

Synthesis and anti-myocarditis activity in a multifunctional lanthanide microporous metal-organic framework with 1D helical chain building units
Provedor de dados: 56
Autores: Hong,Chenglv; Zhou,Xinlang; Huang,Weijian; Shan,Peiren; Dong,Fengquan.
A new microporous lanthanide metal-organic framework, {[Yb(BTB)(H2O) (DEF)2}n (1, DEF=N,N-Diethylformamide), with 1D nano-sized channels has been constructed by bridging helical chain secondary building units with 1,3,5-benzenetrisbenzoic acid (H3BTB) ligand. Structural characterization suggests that this complex crystallizes in the hexagonal space group P6122 and possesses 1D triangular channels with coordinated water molecules pointing to the channel center. In addition, anti-myocarditis properties of compound 1 were evaluated in vivo. The results showed that compound 1 can improve hemodynamic parameters of, and it may be a good therapeutic option for heart failure in the future.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metal-organic framework; Anti-myocarditis; In vivo.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300604

Chemokines, lymphocytes, and HIV
Provedor de dados: 56
Autores: Farber,J.M.
Chemokines are members of a family of more than 30 human cytokines whose best-described activities are as chemotactic factors for leukocytes and that are presumed to be important in leukocyte recruitment and trafficking. While many chemokines can act on lymphocytes, the roles of chemokines and their receptors in lymphocyte biology are poorly understood. The recent discoveries that chemokines can suppress infection by HIV-1 and that chemokine receptors serve, along with CD4, as obligate co-receptors for HIV-1 entry have lent urgency to studies on the relationships between chemokines and lymphocytes. My laboratory has characterized Mig and Crg-2/IP-10, chemokines that are induced by IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; and that specifically target...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Lymphoid cells; HIV-1; Receptors.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100002

Connexin hemichannels and cell-cell channels: comparison of properties
Provedor de dados: 56
Autores: Verselis,V.K.; Trexler,E.B.; Bukauskas,F.F.
Connexin46 (Cx46) forms functional hemichannels in the absence of contact by an apposed hemichannel and we have used these hemichannels to study gating and permeation at the single channel level with high time resolution. Using both cell-attached and -excised patch configurations, we find that single Cx46 hemichannels exhibit some properties expected of half of a gap junction channel, as well as novel properties. Cx46 hemichannels have a large unitary conductance (~300 pS) and a relatively large pore as inferred from permeability to TEA. Both monovalent cations and anions can permeate, but cations are substantially more permeable. The open channel conductance shows marked inward rectification in symmetric salts. We find that the conductance and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Permeability; Channel gating; Connexin46; Patch clamp.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400003

Viability of mesenchymal stem cells during electrospinning
Provedor de dados: 56
Autores: Zanatta,G.; Steffens,D.; Braghirolli,D.I.; Fernandes,R.A.; Netto,C.A.; Pranke,P.
Tissue engineering is a technique by which a live tissue can be re-constructed and one of its main goals is to associate cells with biomaterials. Electrospinning is a technique that facilitates the production of nanofibers and is commonly used to develop fibrous scaffolds to be used in tissue engineering. In the present study, a different approach for cell incorporation into fibrous scaffolds was tested. Mesenchymal stem cells were extracted from the wall of the umbilical cord and mononuclear cells from umbilical cord blood. Cells were re-suspended in a 10% polyvinyl alcohol solution and subjected to electrospinning for 30 min under a voltage of 21 kV. Cell viability was assessed before and after the procedure by exclusion of dead cells using trypan blue...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanotechnology; Tissue engineering; Electrospinning; Stem cells.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200006

Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus
Provedor de dados: 56
Autores: Gonçalves,F.Z.; Lizarte Neto,F.S.; Novais,P.C.; Gattas,D.; Lourenço,L.G.; de Carvalho,C.A.M.; Tirapelli,D.P.C.; Molina,C.A.F.; Tirapelli,L.F.; Tucci Jr,S.
Recent evidence shows that chronic ethanol consumption increases endothelin (ET)-1 induced sustained contraction of trabecular smooth muscle cells of the corpora cavernosa in corpus cavernosum of rats by a mechanism that involves increased expression of ETA and ETB receptors. Our goal was to evaluate the effects of alcohol and diabetes and their relationship to miRNA-155, miRNA-199 and endothelin receptors in the corpus cavernosum and blood of rats submitted to the experimental model of diabetes mellitus and chronic alcoholism. Forty-eight male Wistar rats were divided into four groups: control (C), alcoholic (A), diabetic (D), and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study the protein expression of endothelin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcoholism; Corpus cavernosum; MiRNAs; Endothelin receptors.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300615

Local treatment with ascorbic acid accelerates recovery of post-sutured Achilles tendon in male Wistar rats
Provedor de dados: 56
Autores: Souza,M.; Moraes,S.A.S.; de Paula,D.R.; Maciel,A.A.; Batista,E.J.O.; Silva,D.G.F.; Bahia,C.P.; Oliveira,K.R.H.M.; Herculano,A.M.
Tendon rupture is a very frequent accident involving average people and high-performance athletes. Clinical studies describe tendon recovery as a painful and slow process involving different biochemical and histological events. Ascorbic acid (AA) is a potent antioxidant as well as an important cofactor for collagen synthesis. In the current study, we evaluated if local treatment with AA is able to promote tendon repair in tenotomized rats. Animals were submitted to Achilles tendon rupture followed by surgical suture. Control and AA groups received in loco injection of saline solution (0.9% NaCl) and 30 mM AA, respectively. Histological and functional recovery of Achilles tendon tissue was evaluated at 7, 14, and 21 days post-surgery. Hematoxylin/eosin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tendon recovery; Tenotomy; Ascorbic acid; Tendon suture.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900608

Effects of lead and/or zinc exposure during the second stage of rapid postnatal brain growth on delta-aminolevulinate dehydratase and negative geotaxis of suckling rats
Provedor de dados: 56
Autores: Goulart,E.C.; Pereira,C.A.T.; Garcia,R.C.; Giacomelli,M.B.O.; Rodrigues,A.L.S.
Lead has been shown to produce cognitive and motor deficits in young rats that could be mediated, at least in part, by inhibition of the zinc-containing heme biosynthetic enzyme delta-aminolevulinate dehydratase (ALA-D). In the present study we investigated the effects of lead and/or zinc treatment during the second stage of rapid postnatal brain development on brain, kidney and blood ALA-D specific activity, as well as the negative geotaxis behavior of rats. Eight-day-old Wistar rats were injected intraperitoneally with saline, lead acetate (8 mg/kg) and/or zinc chloride (2 mg/kg) daily for five consecutive days. Twenty-four hours after treatment, ALA-D activity was determined in the absence and presence of DL-dithiothreitol (DTT). The negative geotaxis...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lead acetate; Zinc chloride; Delta-aminolevulinate dehydratase; Negative geotaxis; Postnatal brain growth.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600014

Effects of fluoride on proliferation and mineralization in periodontal ligament cells in vitro
Provedor de dados: 56
Autores: Li,K.Q.; Jia,S.S.; Ma,M.; Shen,H.Z.; Xu,L.; Liu,G.P.; Huang,S.Y.; Zhang,D.S.
Fluoride, which is often added to toothpaste or mouthwash in order to protect teeth from decay, may be a novel therapeutic approach for acceleration of periodontal regeneration. Therefore, we investigated the effects of fluoride on proliferation and mineralization in human periodontal ligament cells in vitro. The periodontal ligament cells were stimulated with various concentrations of NaF added into osteogenic inductive medium. Immunohistochemistry of cell identification, cell proliferation, alkaline phosphatase (ALP) activity assay, Alizarin red S staining and quantitative real-time-polymerase chain reaction (RT-PCR) were performed. Moderate concentrations of NaF (50-500 μmol/L) had pro-proliferation effects, while 500 μmol/L had the best effects. ALP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoride; Periodontitis; Periodontal ligament cells; Mineralization.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800601

POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells
Provedor de dados: 56
Autores: França,M. M.; Abreu,N. P.; Vrechi,T. A. M.; Lotfi,C. F.
During gonad and adrenal development, the POD-1/capsulin/TCF21transcription factor negatively regulates SF-1/NR5A1expression, with higher SF-1 levels being associated with increased adrenal cell proliferation and tumorigenesis. In adrenocortical tumor cells, POD-1 binds to the SF-1 E-box promoter region, decreasing SF-1 expression. However, the modulation of SF-1 expression by POD-1 has not previously been described in normal adrenal cells. Here, we analyzed the basal expression of Pod-1 and Sf-1 in primary cultures of glomerulosa (G) and fasciculata/reticularis (F/R) cells isolated from male Sprague-Dawley rats, and investigated whether POD-1 overexpression modulates the expression of endogenous Sf-1 and its target genes in these cells. POD-1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: POD-1; Tcf21; SF-1; Rat adrenal cell primary culture; Glomerulosa cells; Fasciculata cells.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201087

Sulfated proteoglycans as modulators of neuronal migration and axonal decussation in the developing midbrain
Provedor de dados: 56
Autores: Cavalcante,L.A.; Garcia-Abreu,J.; Mendes,F.A.; Moura Neto,V.; Silva,L.C.F.; Onofre,G.; Weissmüller,G.; Carvalho,S.L.
Proteoglycans are abundant in the developing brain and there is much circumstantial evidence for their roles in directional neuronal movements such as cell body migration and axonal growth. We have developed an in vitro model of astrocyte cultures of the lateral and medial sectors of the embryonic mouse midbrain, that differ in their ability to support neuritic growth of young midbrain neurons, and we have searched for the role of interactive proteins and proteoglycans in this model. Neurite production in co-cultures reveals that, irrespective of the previous location of neurons in the midbrain, medial astrocytes exert an inhibitory or nonpermissive effect on neuritic growth that is correlated to a higher content of both heparan and chondroitin sulfates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astrocytes; Axonal growth; Chondroitin sulfate; Heparan sulfate; Midline; Midbrain.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800005

Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis
Provedor de dados: 56
Autores: Sato,N.S.; Suzuki,T.; Ueda,T.; Watanabe,K.; Hirata,R.D.C.; Hirata,M.H.
Three recombinant antigens of Treponema pallidum Nichols strain were fused with GST, cloned and expressed in Escherichia coli, resulting in high levels of GST-rTp47 and GST-rTp17 expression, and supplementation with arginine tRNA for the AGR codon was needed to obtain GST-rTp15 overexpression. Purified fusion protein yields were 1.9, 1.7 and 5.3 mg/l of cell culture for GST-rTp47, GST-rTp17 and GST-rTp15, respectively. The identities of the antigens obtained were confirmed by automated DNA sequencing using ABI Prism 310 and peptide mapping by Finningan LC/MS. These recombinant antigens were evaluated by immuno-slot blot techniques applied to 137 serum samples from patients with a clinical and laboratory diagnosis of syphilis (61 samples), from healthy...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Treponema pallidum; Cloning; Recombinant antigen; Syphilis; GST.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700002

The hyperinsulinemia produced by concanavalin A in rats is opioid-dependent and hormonally regulated
Provedor de dados: 56
Autores: Francisco-DoPrado,J.; Zambelli,J.E.; Melo-Lima,M.H.; Ribeiro-DaSilva,G.
The present study examines the effect of concanavalin A (Con A) on the blood insulin and glucose levels of rats. Male and female rats treated with Con A (62.5-500 µg/kg) for three days showed a dose- and time-dependent hyperinsulinemia that lasted more than 48 h. Male rats were more sensitive to Con A. Thus, 6 h after treatment with Con A the circulating insulin levels in male rats had increased by 85% (control: 10.2 ± 0.9 mU/l and Con A-treated: 18.8 ± 1 mU/l) compared to only 38% (control: 7.5 ± 0.2 mU/l; Con A-treated: 10.3 ± 0.9 mU/l) in females. An identical response was seen after 12 h. Con A (250 µg/kg) produced time-dependent hypoglycemia in both sexes but more pronounced in males. There was no correlation between the hypoglycemia and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Concanavalin A; Hyperinsulinemia; Blood glucose alterations; Sex hormones; Endogenous opioids; Canatoxin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500015

Arylsulfatase A pseudodeficiency in healthy Brazilian individuals
Provedor de dados: 56
Autores: Pedron,C.G.; Gaspar,P.A.; Giugliani,R.; Pereira,M.L.S.
Molecular alterations associated with arylsulfatase A pseudodeficiency (ASA-PD) were characterized by PCR and restriction endonuclease analysis in a sample of healthy individuals from Brazil. ASA activity was also assayed in all subjects. Two individuals homozygous for the N350S and 1524+95A&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;G mutations were detected, corresponding to a frequency of 1.17% (4 of 324 alleles). The individual frequency of the N350S mutation was 20.7% (71 of 342 alleles) and 7.9% (27 of 342 alleles) for the 1524+95A&lt;FONT FACE="Symbol"&gt;®&lt;/font&gt;G mutation. The frequency of the ASA-PD allele in our population was estimated to be 7.9%. This is the first report of ASA-PD allele frequency in a South American population. In addition,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arylsulfatase A; Pseudodeficiency; Metachromatic leukodystrophy.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800002

Association of leprosy with HLA-DR2 in a Southern Brazilian population
Provedor de dados: 56
The association between HLA specificities and leprosy was investigated in a Southern Brazilian population. One hundred and twenty-one patients and 147 controls were typed for HLA-A, B, Cw, DR and DQ. Patients were subdivided into the following subgroups, according to clinical, histological and immunological criteria: lepromatous (N = 55), tuberculoid (N = 32), dimorphous (N = 20), and indeterminate (N = 14). The frequencies of HLA specificities were compared between the total group of patients and controls, and between the same controls and each subgroup of patients. After correction of the probabilities, deviations were not significant, except for the DR2 specificity, which presented a frequency of 44.2% in the total group of patients and 56.3% in the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HLA; Leprosy; Mycobacterium leprae.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100008

Effect of acute treatment with a water-alcohol extract of Erythrina mulungu on anxiety-related responses in rats
Provedor de dados: 56
Autores: Onusic,G.M.; Nogueira,R.L.; Pereira,A.M.S.; Viana,M.B.
We investigated the effect of acute oral treatment with a water-alcohol extract of the inflorescence of Erythrina mulungu (EM, Leguminosae-Papilionaceae) (100, 200 and 400 mg/kg) on rats submitted to different anxiety models: the elevated T-maze (for inhibitory avoidance and escape measurements), the light/dark transition, and the cat odor test. These models were selected for their presumed capacity to demonstrate specific subtypes of anxiety disorders as recognized in clinical practice. Treatment with 200 mg/kg EM impaired avoidance latencies (avoidance 1 - 200 mg/kg EM: 18 ± 7 s, control group: 40 ± 9 s; avoidance 2 - 200 mg/kg EM: 15 ± 4 s, control group: 110.33 ± 38 s) in a way similar to the reference drug diazepam (avoidance 1: 3 ± 0.79 s; avoidance...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Erythrina mulungu; Anxiety; Animal models.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400011

Effect of chloride dialysate concentration on metabolic acidosis in aintenance hemodialysis patients
Provedor de dados: 56
Autores: Marques,F.O.; Libório,A.B.; Daher,E.F.
Hyperchloremia is one of the multiple etiologies of metabolic acidosis in hemodialysis (HD) patients. The aim of the present study was to determine the influence of chloride dialysate on metabolic acidosis control in this population. We enrolled 30 patients in maintenance HD program with a standard base excess (SBE) ≤2 mEq/L and urine output of less than 100 mL/24 h. The patients underwent dialysis three times per week with a chloride dialysate concentration of 111 mEq/L for 4 weeks, and thereafter with a chloride dialysate concentration of 107 mEq/L for the next 4 weeks. Arterial blood was drawn immediately before the second dialysis session of the week at the end of each phase, and the Stewart physicochemical approach was applied. The strong ion gap...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chloride dialysate; Hemodialysis; Metabolic acidosis; Stewart model.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000012

Anxiety and depression symptoms in recurrent painful renal lithiasis colic
Provedor de dados: 56
Autores: Diniz,D.H.M.P.; Blay,S.L.; Schor,N.
Several studies have reported that symptoms of anxiety and depression are significantly associated with diseases characterized by painful crises. However, there is little information about the psychological aspects of recurrent painful episodes of renal stone disease. Our objective was to evaluate the association of symptoms of anxiety, depression and recurrent painful renal colic in a case-control study involving 64 subjects (32 cases/32 controls) matched for age and sex. Cases were outpatients with a confirmed diagnosis of nephrolithiasis as per their case history, physical examination, image examination and other laboratory exams. Patients had a history of at least two episodes within a 3-year period, and were currently in an intercrisis interval. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal colic; Lithiasis; Symptoms of anxiety; Symptoms of depression.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700009

Use of single photon emission computed tomography and magnetic resonance to evaluate central nervous system involvement in patients with juvenile systemic lupus erythematosus
Provedor de dados: 56
Autores: Prismich,G.; Hilário,M.O.E.; Len,C.A.; Terreri,M.T.; Quaresma,M.R.; Alonso,G.; Sevillano,M.M.; Lederman,H.M.
The objective of the present study was to identify the single photon emission computed tomography (SPECT) and magnetic resonance (MR) findings in juvenile systemic lupus erythematosus (JSLE) patients with CNS involvement and to try to correlate them with neurological clinical history data and neurological clinical examination. Nineteen patients with JSLE (16 girls and 3 boys, mean age at onset 9.2 years) were submitted to neurological examination, electroencephalography, cerebrospinal fluid analysis, SPECT and MR. All the evaluations were made separately within a period of 15 days. SPECT and MR findings were analyzed independently by two radiologists. Electroencephalography and cerebrospinal fluid analysis revealed no relevant alterations. Ten of 19...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic lupus erythematosus; Central nervous system; Single photon emission computed tomography; Magnetic resonance; Neurological clinical history.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700007

Multicellular spheroids of bone marrow stromal cells: a three-dimensional in vitro culture system for the study of hematopoietic cell migration
Provedor de dados: 56
Autores: Rossi,M.I.D.; Barros,A.P.D.N.; Baptista,L.S.; Garzoni,L.R.; Meirelles,M.N.; Takiya,C.M.; Pascarelli,B.M.O.; Dutra,H.S.; Borojevic,R.
Cell fate decisions are governed by a complex interplay between cell-autonomous signals and stimuli from the surrounding tissue. In vivo cells are connected to their neighbors and to the extracellular matrix forming a complex three-dimensional (3-D) microenvironment that is not reproduced in conventional in vitro systems. A large body of evidence indicates that mechanical tension applied to the cytoskeleton controls cell proliferation, differentiation and migration, suggesting that 3-D in vitro culture systems that mimic the in vivo situation would reveal biological subtleties. In hematopoietic tissues, the microenvironment plays a crucial role in stem and progenitor cell survival, differentiation, proliferation, and migration. In adults, hematopoiesis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multicellular spheroid; Three-dimensional culture; Cell migration; Bone marrow.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000002

CYP1A1 and GSTP1 polymorphisms in an oral cancer case-control study
Provedor de dados: 56
Autores: Leichsenring,A.; Losi-Guembarovski,R.; Maciel,M.E.; Losi-Guembarovski,A.; Oliveira,B.W.; Ramos,G.; Cavalcanti,T.C.S.; Bicalho,M.G.; Cavalli,I.J.; Cólus,I.M.S.; Ribeiro,E.M.S.F.
CYP1A1 and GSTP1 polymorphisms have been associated with a higher risk to develop several cancers, including oral squamous cell carcinoma (OSCC), which is closely related to tobacco and alcohol consumption. Both genes code for enzymes that have an important role in activating or detoxifying carcinogenic elements found in tobacco and other compounds, and polymorphic variants of these genes may result in alterations of the enzymatic activity. The CYP1A1 gene codes for the enzyme aryl hydrocarbon hydroxylase, which is responsible for the metabolism of polycyclic aromatic hydrocarbons. The investigated polymorphism, Ile/Val, seems to increase the activity of the enzyme in homozygous individuals, leading to an accumulation of carcinogens. The Ile/Val...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral carcinoma; Biometabolism genes; CYP1A1; GSTP1; Gene polymorphism.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200007

Selective destruction of nigrostriatal dopaminergic neurons does not alter [3H]-ryanodine binding in rat striatum
Provedor de dados: 56
Autores: Noël,F.; Geurts,M.; Maloteaux,J.-M.
Dopamine nigrostriatal neurons are important for motor control and may contain a particularly dense population of ryanodine receptors involved in the control of dopamine release. To test this hypothesis, we used a classical model of unilateral selective lesion of these neurons in rats based on 6-hydroxydopamine (6-OHDA) injection into the substantia nigra. Binding of [3H]-GBR 12935, used as a presynaptic marker since it labels specifically the dopamine uptake complex, was dramatically decreased by 83-100% in striatum homogenates after 6-OHDA lesion. On the contrary, no reduction of [3H]-ryanodine binding was observed. The present data indicate that [3H]-ryanodine binding sites present in rat striatum are not preferentially localized in dopaminergic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ryanodine; Striatum; 6-hydroxydopamine; Dopamine neurons; Neuroleptic malignant syndrome.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200013

The hyperglycemia induced by angiotensin II in rats is mediated by AT1 receptors
Provedor de dados: 56
Autores: Machado,L.J.C.; Marubayashi,U.; Reis,A.M.; Coimbra,C.C.
We have shown that the renin-angiotensin system (RAS) is involved in glucose homeostasis during acute hemorrhage. Since almost all of the physiological actions described for angiotensin II were mediated by AT1 receptors, the present experiments were designed to determine the participation of AT1 receptors in the hyperglycemic action of angiotensin II in freely moving rats. The animals were divided into two experimental groups: 1) animals submitted to intravenous administration of angiotensin II (0.96 nmol/100 g body weight) which caused a rapid increase in plasma glucose reaching the highest values at 5 min after the injection (33% of the initial values, P&amp;lt;0.01), and 2) animals submitted to intravenous administration of DuP-753 (losartan), a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glycemia; Angiotensin II; DuP-753; AT1; Receptor.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000018

Interactions between intracellular Ca2+ stores: Ca2+ released from the NAADP pool potentiates cADPR-induced Ca2+ release
Provedor de dados: 56
Autores: Chini,E.N.
Cells possess multiple intracellular Ca2+-releasing systems. Sea urchin egg homogenates are a well-established model to study intracellular Ca2+ release. In the present study the mechanism of interaction between three intracellular Ca2+ pools, namely the nicotinic acid adenine dinucleotide phosphate (NAADP), the cyclic ADP-ribose (cADPR) and the inositol 1',4',5'-trisphosphate (IP3)-regulated Ca2+ stores, is explored. The data indicate that the NAADP Ca2+ pool could be used to sensitize the cADPR system. In contrast, the IP3 pool was not affected by the Ca2+ released by NAADP. The mechanism of potentiation of the cADPR-induced Ca2+ release, promoted by Ca2+ released from the NAADP pool, is mediated by the mechanism of Ca2+-induced Ca2+ release. These data...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CADPR; NAADP; Calcium; Sea urchin eggs; Fertilization; IP3.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500005

Efficacy of removal of cariogenic bacteria and carious dentin by ablation using different modes of Er:YAG lasers
Provedor de dados: 56
Autores: Baraba,A.; Kqiku,L.; Gabrić,D.; Verzak,Ž.; Hanscho,K.; Miletić,I.
The primary objective of this in vitro study was to evaluate the efficiency of removal of cariogenic bacteria and carious dentin by ablation using two lasers: fluorescence-feedback controlled (FFC) Er:YAG laser and different pulses of Er:YAG laser based on variable square pulse technology (VSPt). The secondary objective was to measure the temperature during laser ablation of carious tissue. Seventy-two extracted human molars were used in this study. Sixty teeth with carious dentin were randomly divided into four experimental groups according to the treatment for caries removal: group 1: 400 µs (FFC group); group 2: super short pulse (SSP group, 50 µs pulse); group 3: medium short pulse (MSP group, 100 µs pulse); group 4: short pulse (SP group, 300 µs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dental caries; Er:YAG laser; Ablation; Real-time PCR; Thermography.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300605

Impaired regeneration of dystrophin-deficient muscle fibers is caused by exhaustion of myogenic cells
Provedor de dados: 56
Autores: Luz,M.A.M.; Marques,M.J.; Santo Neto,H.
Duchenne muscular dystrophy is one of the most devastating myopathies. Muscle fibers undergo necrosis and lose their ability to regenerate, and this may be related to increased interstitial fibrosis or the exhaustion of satellite cells. In this study, we used mdx mice, an animal model of Duchenne muscular dystrophy, to assess whether muscle fibers lose their ability to regenerate after repeated cycles of degeneration-regeneration and to establish the role of interstitial fibrosis or exhaustion of satellite cells in this process. Repeated degenerative-regenerative cycles were induced by the injection of bupivacaine (33 mg/kg), a myotoxic agent. Bupivacaine was injected weekly into the right tibialis anterior muscle of male, 8-week-old mdx (N = 20) and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mdx fiber regeneration; Bupivacaine; Duchenne muscular dystrophy; Muscle degeneration.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600009

Mathematical modeling of electro-rotation spectra of small particles in liquid solutions: Application to human erythrocyte aggregates
Provedor de dados: 56
Autores: Zehe,A.; Ramírez,A.; Starostenko,O.
Electro-rotation can be used to determine the dielectric properties of cells, as well as to observe dynamic changes in both dielectric and morphological properties. Suspended biological cells and particles respond to alternating-field polarization by moving, deforming or rotating. While in linearly polarized alternating fields the particles are oriented along their axis of highest polarizability, in circularly polarized fields the axis of lowest polarizability aligns perpendicular to the plane of field rotation. Ellipsoidal models for cells are frequently applied, which include, beside sphere-shaped cells, also the limiting cases of rods and disks. Human erythrocyte cells, due to their particular shape, hardly resemble an ellipsoid. The additional effect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electro-rotation spectra; Induced dipole moment; Mathematical modeling; Erythrocyte aggregates.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200003

The use of non-human primates as animal models for the study of hepatitis viruses
Provedor de dados: 56
Autores: Vitral,C.L.; Yoshida,C.F.T.; Gaspar,A.M.C.
Hepatitis viruses belong to different families and have in common a striking hepatotropism and restrictions for propagation in cell culture. The transmissibility of hepatitis is in great part limited to non-human primates. Enterically transmitted hepatitis viruses (hepatitis A virus and hepatitis E virus) can induce hepatitis in a number of Old World and New World monkey species, while the host range of non-human primates susceptible to hepatitis viruses transmitted by the parenteral route (hepatitis B virus, hepatitis C virus and hepatitis delta virus) is restricted to few species of Old World monkeys, especially the chimpanzee. Experimental studies on non-human primates have provided an invaluable source of information regarding the biology and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis viruses; Non-human primates; Animal model; Experimental infection.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800003

Co-transfection of hepatocyte growth factor and truncated TGF-β type II receptor inhibit scar formation
Provedor de dados: 56
Autores: Xu,Ji-Hua; Zhao,Wan-Yi; Fang,Qing-Qing; Wang,Xiao-Feng; Zhang,Ding-Ding; Hu,Yan-Yan; Zheng,Bin; Tan,Wei-Qiang.
Wound scarring remains a major challenge for plastic surgeons. Transforming growth factor (TGF)-β plays a key role in the process of scar formation. Previous studies have demonstrated that truncated TGF-β type II receptor (t-TGF-βRII) is unable to continue signal transduction but is still capable of binding to TGF-β, thereby blocking the TGF-β signaling pathway. Hepatocyte growth factor (HGF) is a multifunctional growth factor that promotes tissue regeneration and wound healing. Theoretically, the combination of HGF and t-TGF-βRII would be expected to exert a synergistic effect on promoting wound healing and reducing collagen formation. In the present study, lentivirus-mediated transfection of the two genes (t-TGF-βRII/HGF) into fibroblasts in vitro and in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wound healing; Truncated transforming growth factor-β type II receptor; Hepatocyte growth factor; Scar; Rat; Lentivirus.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100611

Furosemide is associated with acute kidney injury in critically ill patients
Provedor de dados: 56
Autores: Levi,T.M.; Rocha,M.S.; Almeida,D.N.; Martins,R.T.C.; Silva,M.G.C.; Santana,N.C.P.; Sanjuan,I.T.; Cruz,C.M.S.
Acute kidney injury (AKI) is common in critically ill patients. Diuretics are used without any evidence demonstrating a beneficial effect on renal function. The objective of the present study is to determine the incidence of AKI in an intensive care unit (ICU) and if there is an association between the use of furosemide and the development of AKI. The study involved a hospital cohort in which 344 patients were consecutively enrolled from January 2010 to January 2011. A total of 132 patients (75 females and 57 males, average age 64 years) remained for analysis. Most exclusions were related to ICU discharge in the first 24 h. Laboratory, sociodemographic and clinical data were collected until the development of AKI, medical discharge or patient death. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute renal failure; Critically ill patients; Furosemide; Septic shock.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900006

Incidence, risk factors and prognostic factors of acute renal failure in patients admitted to an intensive care unit
Provedor de dados: 56
Autores: Mataloun,S.E.; Machado,F.R.; Senna,A.P.R.; Guimarães,H.P.; Amaral,J.L.G.
The objective of the present study was to assess the incidence, risk factors and outcome of patients who develop acute renal failure (ARF) in intensive care units. In this prospective observational study, 221 patients with a 48-h minimum stay, 18-year-old minimum age and absence of overt acute or chronic renal failure were included. Exclusion criteria were organ donors and renal transplantation patients. ARF was defined as a creatinine level above 1.5 mg/dL. Statistics were performed using Pearsons' chi2 test, Student t-test, and Wilcoxon test. Multivariate analysis was run using all variables with P &lt; 0.1 in the univariate analysis. ARF developed in 19.0% of the patients, with 76.19% resulting in death. Main risk factors (univariate analysis) were:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute renal failure; Risk factors; Prognostic factors; Intensive care unit; Mortality.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001000010

GABA in the central amygdaloid nucleus modulates the electrolyte excretion and hormonal responses to blood volume expansion in rats
Provedor de dados: 56
Autores: Margatho,L.O.; Elias,L.L.K.; Antunes-Rodrigues,J.
We investigated the involvement of GABAergic mechanisms of the central amygdaloid nucleus (CeA) in unanesthetized rats subjected to acute isotonic or hypertonic blood volume expansion (BVE). Male Wistar rats bearing cannulas unilaterally implanted in the CeA were treated with vehicle, muscimol (0.2 nmol/0.2 µL) or bicuculline (1.6 nmol/0.2 µL) in the CeA, followed by isotonic or hypertonic BVE (0.15 or 0.3 M NaCl, 2 mL/100 g body weight over 1 min). The vehicle-treated group showed an increase in sodium excretion, urinary volume, plasma oxytocin (OT), and atrial natriuretic peptide (ANP) levels compared to control rats. Muscimol reduced the effects of BVE on sodium excretion (isotonic: 2.4 ± 0.3 vs vehicle: 4.8 ± 0.2 and hypertonic: 4.0 ± 0.7 vs vehicle:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Central amygdaloid nucleus; Natriuresis; Urine output; Oxytocin; Atrial natriuretic peptide.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100016

Automated grading of left ventricular segmental wall motion by an artificial neural network using color kinesis images
Provedor de dados: 56
Autores: Murta Jr.,L.O.; Ruiz,E.E.S.; Pazin-Filho,A.; Schmidt,A.; Almeida-Filho,O.C.; Simões,M.V.; Marin-Neto,J.A.; Maciel,B.C.
The present study describes an auxiliary tool in the diagnosis of left ventricular (LV) segmental wall motion (WM) abnormalities based on color-coded echocardiographic WM images. An artificial neural network (ANN) was developed and validated for grading LV segmental WM using data from color kinesis (CK) images, a technique developed to display the timing and magnitude of global and regional WM in real time. We evaluated 21 normal subjects and 20 patients with LVWM abnormalities revealed by two-dimensional echocardiography. CK images were obtained in two sets of viewing planes. A method was developed to analyze CK images, providing quantitation of fractional area change in each of the 16 LV segments. Two experienced observers analyzed LVWM from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artificial neural network; Color kinesis images; Left ventricular function.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100001

There are more asthmatics in homes with high cockroach infestation
Provedor de dados: 56
Autores: Sarinho,E.; Schor,D.; Veloso,M.A.; Rizzo,J.A.
Although asthma has been commonly associated with sensitivity to cockroaches, a clear causal relationship between asthma, allergy to cockroaches and exposure levels has not been extensively investigated. The objective of the present study was to determine whether asthma occurs more frequently in children living in homes with high cockroach infestation. The intensity of household infestation was assessed by the number of dead insects after professional pesticide application. Children living in these houses in the metropolitan area of Recife, PE, were diagnosed as having asthma by means of a questionnaire based on the ISAAC study. All children had physician-diagnosed asthma and at least one acute exacerbation in the past year. Children of both sexes aged 4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cockroaches; Asthma; Atopy; Public health; Plague control.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400007

Propofol pretreatment attenuates lipopolysaccharide-induced acute lung injury in rats by activating the phosphoinositide-3-kinase/Akt pathway
Provedor de dados: 56
Autores: Zhao,L.L.; Hu,G.C.; Zhu,S.S.; Li,J.F.; Liu,G.J.
The aim of this study was to investigate the effect of propofol pretreatment on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the role of the phosphoinositide-3-kinase/protein kinase B (PI3K/Akt) pathway in this procedure. Survival was determined 48 h after LPS injection. At 1 h after LPS challenge, the lung wet- to dry-weight ratio was examined, and concentrations of protein, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in bronchoalveolar lavage fluid (BALF) were determined using the bicinchoninic acid method or ELISA. Lung injury was assayed via lung histological examination. PI3K and p-Akt expression levels in the lung tissue were determined by Western blotting. Propofol pretreatment prolonged survival, decreased the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute lung injury; Propofol; Lipopolysaccharide; PI3K/Akt pathway.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201062

Effect of a rosmarinic acid supplemented hemodialysis fluid on inflammation of human vascular endothelial cells
Provedor de dados: 56
Autores: Wang,W-J.; Cheng,M-H.; Lin,J-H.; Weng,C-S.
Chronic systemic inflammation and repetitive damage of vascular endothelia by incompatible dialysis system are probable causes of cardiovascular disease in patients on dialysis. The present study aimed to assess in vitro biocompatibility and anti-inflammatory effect of hemodialysis fluid supplemented with rosmarinic acid (RA) using human umbilical vein endothelial cells (HUVEC). HUVECs (5×106 cells/mL) were pre-exposed to 1 μg/mL of lipopolysaccharides (LPS) and incubated with RA-supplemented hemodialysis fluid (HDF). Cytotoxicity was assessed qualitatively by morphologic assessment and quantitatively by MTT assay. Expressions of proinflammatory mediators were assessed using quantitative real-time PCR and production of NO was quantified. Phosphorylation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosmarinic acid; Hemodialysis fluid; Anti-inflammation; Endothelial cells; LPS.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200606

cDNA and deduced primary structure of basic phospholipase A2 with neurotoxic activity from the venom secretion of the Crotalus durissus collilineatus rattlesnake
Provedor de dados: 56
Autores: Fagundes,F.H.R.; Oliveira,M.; Huancahuire-Vega,S.; Romero-Vargas,F.F.; Ponce-Soto,L.A.; Marangoni,S.
To illustrate the construction of precursor complementary DNAs, we isolated mRNAs from whole venom samples. After reverse transcription polymerase chain reaction (RT-PCR), we amplified the cDNA coding for a neurotoxic protein, phospholipase A2 D49 (PLA2 D49), from the venom of Crotalus durissus collilineatus (Cdc PLA2). The cDNA encoding Cdc PLA2 from whole venom was sequenced. The deduced amino acid sequence of this cDNA has high overall sequence identity with the group II PLA2 protein family. Cdc PLA2 has 14 cysteine residues capable of forming seven disulfide bonds that characterize this group of PLA2 enzymes. Cdc PLA2 was isolated using conventional Sephadex G75 column chromatography and reverse-phase high performance liquid chromatography (RP-HPLC)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cdc PLA2; CDNA; Chick biventer cervicis; Crotalus durissus collilineatus; Mass spectrometry MALDI-TOF; Neurotoxic agents.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300007

A new model for the immobilization of the rat hind limb
Provedor de dados: 56
Autores: Coutinho,E.L.; Gomes,A.R.S.; França,C.N.; Salvini,T.F.
An alternative device for the immobilization of the hind limb of the rat was developed to study the effects of chronic disuse on the soleus and tibialis anterior muscles, maintained for 3 weeks in the shortening and the stretching positions, respectively. The proposed device is made of steel mesh and cotton materials, and has some advantages when compared to cast or plaster cast: it is cheaper, lighter (12 g or 4% of the body weight of the rat) and the same unit can be easily adjusted and used several times in the same animal or in animals of similar size. Immobilization is also useful to restrain the movements of the hip, knee, and ankle joints. Male rats (291 ± 35 g and aged 14 ± 2 weeks) were used to develop and test the model. The soleus muscle of 18...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Muscle immobilization; Muscle shortening; Muscle stretching; Soleus muscle; Tibialis anterior; Skeletal muscle.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100010

Maintenance of the corneal epithelium is carried out by germinative cells of its basal stratum and not by presumed stem cells of the limbus
Provedor de dados: 56
Autores: Haddad,A.; Faria-e-Sousa,S.J.
The purpose of this investigation was to analyze the proliferative behavior of rabbit corneal epithelium and establish if any particular region was preferentially involved in epithelial maintenance. [3H]-thymidine was injected intravitreally into both normal eyes and eyes with partially scraped corneal epithelium. Semithin sections of the anterior segment were evaluated by quantitative autoradiography. Segments with active replication (on) and those with no cell division (off) were intermingled in all regions of the tissue, suggesting that the renewal of the epithelial surface of the cornea followed an on/off alternating pattern. In the limbus, heavy labeling of the outermost layers was observed, coupled with a few or no labeled nuclei in the basal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cornea; Epithelium; Autoradiography; Germinative cells; Stem cells; Limbus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600470

Serum laminin, type IV collagen and hyaluronan as fibrosis markers in non-alcoholic fatty liver disease
Provedor de dados: 56
Autores: Santos,V.N. dos; Leite-Mór,M.M.B.; Kondo,M.; Martins,J.R.; Nader,H.; Lanzoni,V.P.; Parise,E.R.
Hepatic fibrosis in patients with non-alcoholic fatty liver disease is associated with progression of the disease. In the present study, we analyzed the discriminative ability of serum laminin, type IV collagen and hyaluronan levels to predict the presence of fibrosis in these patients. In this preliminary report, we studied 30 overweight patients divided into two groups according to the absence (group I, N = 19) or presence (group II, N = 11) of fibrosis in a liver biopsy. Triglycerides, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidade, hyaluronan (noncompetitive fluoroassay), type IV collagen, and laminin (ELISA) were determined. Group II presented significantly higher mean laminin, hyaluronan, type IV collagen, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic fibrosis; Hyaluronan; Laminin; Non-alcoholic fatty liver disease; Non-alcoholic steatohepatitis; Type IV collagen.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500012

Stabilization of serum antibody responses triggered by initial mucosal contact with the antigen independently of oral tolerance induction
Provedor de dados: 56
Autores: Verdolin,B.A.; Ficker,S.M.; Faria,A.M.C.; Vaz,N.M.; Carvalho,C.R.
Initial contacts with a T-dependent antigen by mucosal routes may result in oral tolerance, defined as the inhibition of specific antibody formation after subsequent parenteral immunizations with the same antigen. We describe here an additional and permanent consequence of these initial contacts, namely, the blockade of secondary-type responsiveness to subsequent parenteral contacts with the antigen. When repeatedly boosted ip with small doses (3 µg) of ovalbumin (OVA) (or lysozyme), primed B6D2F1 mice showed progressively higher antibody responses. In contrast, mice primed after a single oral exposure to the antigen, although repeatedly boosted, maintained their secondary antibody titers on a level which was inversely proportional to the dose of antigen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral tolerance; Mucosal immunity; Stability; Memory.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200008

Ventilation distribution as a contributor to the functional exercise capacity in patients with systemic sclerosis-associated interstitial lung disease without pulmonary hypertension
Provedor de dados: 56
Autores: Andrade,F.M.; Oliveira,A.D.; Lopes,A.J.
Phenotypic differences have been described between patients with systemic sclerosis (SSc)-associated interstitial lung disease (ILD) and SSc-associated pulmonary hypertension, including performance differences in the 6-min walk test (6MWT). Moreover, the correlations between the 6MWT and traditional pulmonary function tests (PFTs) are weak, indicating the need to search for new parameters that explain exercise performance. Thus, our objective was to evaluate the impact of ventilation distribution heterogeneity assessed by the nitrogen single-breath washout (N2SBW) test and peripheral muscle dysfunction on the exercise capacity in patients with SSc-ILD and limited involvement of the pulmonary parenchyma. In this cross-sectional study, 20 women with SSc-ILD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic sclerosis; Respiratory function tests; Ventilation; Nitrogen single-breath washout test; Exercise; Six-minute walk test.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800601

Acute stress-induced antinociception is cGMP-dependent but heme oxygenase-independent
Provedor de dados: 56
Autores: Carvalho-Costa,P.G.; Branco,L.G.S.; Leite-Panissi,C.R.A.
Endogenous carbon monoxide (CO), which is produced by the enzyme heme oxygenase (HO), participates as a neuromodulator in physiological processes such as thermoregulation and nociception by stimulating the formation of 3′,5′-cyclic guanosine monophosphate (cGMP). In particular, the acute physical restraint-induced fever of rats can be blocked by inhibiting the enzyme HO. A previous study reported that the HO-CO-cGMP pathway plays a key phasic antinociceptive role in modulating noninflammatory acute pain. Thus, this study evaluated the involvement of the HO-CO-cGMP pathway in antinociception induced by acute stress in male Wistar rats (250-300 g; n=8/group) using the analgesia index (AI) in the tail flick test. The results showed that antinociception...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heme oxygenase; Carbon monoxide; CGMP; Lateral cerebral ventricle; Acute stress; Antinociception.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201057

Expression analysis of miRNA and target mRNAs in esophageal cancer
Provedor de dados: 56
Autores: Meng,X.R.; Lu,P.; Mei,J.Z.; Liu,G.J.; Fan,Q.X.
We aimed to investigate miRNAs and related mRNAs through a network-based approach in order to learn the crucial role that they play in the biological processes of esophageal cancer. Esophageal squamous-cell carcinoma (ESCC) and adenocarcinoma (EAC)-related miRNA and gene expression data were downloaded from the Gene Expression Omnibus database, and differentially expressed miRNAs and genes were selected. Target genes of differentially expressed miRNAs were predicted and their regulatory networks were constructed. Differentially expressed miRNA analysis selected four miRNAs associated with EAC and ESCC, among which hsa-miR-21 and hsa-miR-202 were shared by both diseases. hsa-miR-202 was reported for the first time to be associated with esophageal cancer in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esophageal adenocarcinoma; Esophageal squamous-cell carcinoma; MiRNA expression network; Pathway; Gene ontology.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900811

Residual ß-cell function and microvascular complications in type 1 diabetic patients
Provedor de dados: 56
Autores: Gomes,M.B.; Gonçalves,M.F.R.; Neves,R.; Cohen,C.; Albanesi,F.M.
To determine the influence of residual ß-cell function on retinopathy and microalbuminuria we measured basal C-peptide in 50 type 1 diabetic outpatients aged 24.96 ± 7.14 years, with a duration of diabetes of 9.1 ± 6.2 years. Forty-three patients (86%) with low C-peptide (&amp;lt;0.74 ng/ml) had longer duration of diabetes than 7 patients (14%) with high C-peptide (&lt;FONT FACE="Symbol"&gt;³&lt;/font&gt;0.74 ng/ml) (9 (2-34) vs 3 (1-10) years, P = 0.01) and a tendency to high glycated hemoglobin (HBA1) (8.8 (6-17.9) vs 7.7 (6.9-8.7)%, P = 0.08). Nine patients (18%) had microalbuminuria (two out of three overnight urine samples with an albumin excretion rate (AER) &lt;FONT FACE="Symbol"&gt;³&lt;/font&gt;20 and &amp;lt;200 µg/min) and 13 (26%) had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-peptide; Microalbuminuria; Retinopathy; Diabetes type 1.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200008

Liver cancer stem cells are selectively enriched by low-dose cisplatin
Provedor de dados: 56
Autores: Zhang,H.; Chang,W.J.; Li,X.Y.; Zhang,N.; Kong,J.J.; Wang,Y.F.
Accumulating evidence has indicated the importance of cancer stem cells in carcinogenesis. The goal of the present study was to determine the effect of low-dose cisplatin on enriched liver cancer stem cells (LCSCs). Human hepatoblastoma HepG2 cells were treated with concentrations of cisplatin ranging from 1 to 5 μg/mL. Cell survival and proliferation were evaluated using a tetrazolium dye (MTT) assay. LCSCs were identified using specific markers, namely aldehyde dehydrogenase-1 (ALDH1) and CD133. The percentage of ALDH1+ or CD133+ cells was examined by flow cytometric analysis. The expression of ALDH1 and/or CD133 in HepG2 cells was determined by immunocytochemical analysis. Low-dose cisplatin treatment significantly decreased cell survival in HepG2 cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cisplatin; Liver cancer stem cell; HepG2; ALDH1; CD133.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600478

Immunohistochemical demonstration of TGF-ß and decorin in paracoccidioidal granulomas
Provedor de dados: 56
Autores: Nishikaku,A.S.; Burger,E.
Different patterns of granulomas have been observed in 6- to 8-week-old mice after ip inoculation with 5 x 10(6) yeast cells of Paracoccidioides brasiliensis. Transforming growth factor-ß (TGF-ß) is a cytokine that has been shown to participate in fibrosis and granuloma formation; its activities seem to be modulated by the small proteoglycan decorin. In the present study, TGF-ß and decorin expression in epiploon granulomas was assessed by immunohistochemistry in susceptible (B10.A) and resistant (A/J) mice after 15, 30, 120 and 150 days of P. brasiliensis ip infection. The epiploon was collected, fixed in Methacarn solution and embedded in paraffin, and 5-µm thick sections were used for immunohistochemical analysis employing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TGF-ß; Decorin; Paracoccidioides brasiliensis; Granulomatous lesions; Susceptibility and resistance.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800014

Sex hormone modulation of serotonin-induced coronary vasodilation in isolated heart
Provedor de dados: 56
Autores: Moysés,M.R.; Barker,L.A.; Cabral,A.M.
The present study was designed to evaluate the differences in the coronary vasodilator actions of serotonin (5-HT) in isolated heart obtained from naive or castrated male and female rats that were treated with either estrogen or testosterone. Hearts from 12 groups of rats were used: male and female naive animals, castrated, castrated and treated with 17ß-estradiol (0.5 µg kg-1 day-1) for 7 or 30 days, and castrated and treated with testosterone (0.5 mg kg-1 day-1) for 7 or 30 days. After treatment, the vascular reactivity of the coronary bed was evaluated. Baseline coronary perfusion pressure (CPP) was determined and dose-response curves to 5-HT were generated. Baseline CPP differed between male (70 ± 6 mmHg, N = 10) and female (115 ± 6 mmHg, N = 12) naive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary circulation; Endothelial function; Estradiol; Serotonin; Smooth muscle.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700014

Noninvasive classification of liver disease in asymptomatic and oligosymptomatic male alcoholics
Provedor de dados: 56
Autores: Borini,P.; Guimarães,R.C.
The predominant type of liver alteration in asymptomatic or oligosymptomatic chronic male alcoholics (N = 169) admitted to a psychiatric hospital for detoxification was classified by two independent methods: liver palpation and multiple quadratic discriminant analysis (QDA), the latter applied to two parameters reported by the patient (duration of alcoholism and daily amount ingested) and to the data obtained from eight biochemical blood determinations (total bilirubin, alkaline phosphatase, glycemia, potassium, aspartate aminotransferase, albumin, globulin, and sodium). All 11 soft and sensitive, and 13 firm and sensitive livers formed fully concordant groups as determined by QDA. Among the 22 soft and not sensitive livers, 95% were concordant by QDA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcoholism; Hepatopathy; Noninvasive classification; Liver palpation; Laboratory exams; Discriminant analysis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000013

Heterotopic transplantation of glycerin-preserved trachea: effect of respiratory epithelium desquamation on acute rejection
Provedor de dados: 56
Autores: Saueressig,M.G.; Edelweiss,M.I.A.; Souza,F.H.; Moreschi,A.H.; Savegnago,F.L.; Macedo Neto,A.V.
An effective preservation method and decreased rejection are essential for tracheal transplantation in the reconstruction of large airway defects. Our objective in the present study was to evaluate the antigenic properties of glycerin-preserved tracheal segments. Sixty-one tracheal segments (2.4 to 3.1 cm) were divided into three groups: autograft (N = 21), fresh allograft (N = 18) and glycerin-preserved allograft (N = 22). Two segments from different groups were implanted into the greater omentum of dogs (N = 31). After 28 days, the segments were harvested and analyzed for mononuclear infiltration score and for the presence of respiratory epithelium. The fresh allograft group presented the highest score for mononuclear infiltration (1.78 ± 0.43, P &lt;=...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Graft rejection; Trachea; Transplantation; Glycerol; Respiratory mucosa.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700010

Immunity and immunosuppression in experimental visceral leishmaniasis
Provedor de dados: 56
Autores: Goto,H.; Lindoso,J.A.L.
Leishmaniasis is a disease caused by protozoa of the genus Leishmania, and visceral leishmaniasis is a form in which the inner organs are affected. Since knowledge about immunity in experimental visceral leishmaniasis is poor, we present here a review on immunity and immunosuppression in experimental visceral leishmaniasis in mouse and hamster models. We show the complexity of the mechanisms involved and differences when compared with the cutaneous form of leishmaniasis. Resistance in visceral leishmaniasis involves both CD4+ and CD8+ T cells, and interleukin (IL)-2, interferon (IFN)- gamma, and IL-12, the latter in a mechanism independent of IFN- gamma and linked to transforming growth factor (TGF)-ß production. Susceptibility involves IL-10 but not IL-4,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Immunosuppression; T cell; Cytokines; Mice; Hamster.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400020

Sildenafil decreases rat tracheal hyperresponsiveness to carbachol and changes canonical transient receptor potential gene expression after antigen challenge
Provedor de dados: 56
Autores: Sousa,C.T.; Brito,T.S.; Lima,F.J.B.; Siqueira,R.J.B.; Magalhães,P.J.C.; Lima,A.A.M.; Santos,A.A.; Havt,A.
Inhibition of type-5 phosphodiesterase by sildenafil decreases capacitative Ca2+ entry mediated by transient receptor potential proteins (TRPs) in the pulmonary artery. These families of channels, especially the canonical TRP (TRPC) subfamily, may be involved in the development of bronchial hyperresponsiveness, a hallmark of asthma. In the present study, we evaluated i) the effects of sildenafil on tracheal rings of rats subjected to antigen challenge, ii) whether the extent of TRPC gene expression may be modified by antigen challenge, and iii) whether inhibition of type-5 phosphodiesterase (PDE5) may alter TRPC gene expression after antigen challenge. Sildenafil (0.1 µM to 0.6 mM) fully relaxed carbachol-induced contractions in isolated tracheal rings...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Ovalbumin; Capacitative Ca2+ entry; Transient receptor potential proteins.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600010

Comparative study of IgA nephropathy with and without crescents
Provedor de dados: 56
Autores: Bitencourt-Dias,C.; Bahiense-Oliveira,M.; Saldanha,L.B.; Barros,R.T.; Woronik,V.
Glomerular crescents were analyzed as a prognostic factor in retrospectively reviewed data from 144 patients with biopsy-proven IgA nephropathy. Crescents were found in 26 (18%) patients, and detected in 2 to 100% of glomeruli in each specimen. In 5% of the patients more than 50% of the glomeruli were affected. Thirty patients with IgA nephropathy without crescents were studied as a control group. Mean age was 30.3 ± 9.4 and 30.2 ± 12.0 years for the patients with and without crescents, respectively, and males prevailed in both groups. The length of follow-up was 23.2 ± 41.6 months for patients with crescents and 29.3 ± 35.3 months for patients without crescents. Eighty percent of the patients with crescents were hypertensive, compared to 27% of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IgA nephropathy; Crescents; Renal failure; End-stage renal disease; Creatinine.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900012

Early neonatal echocardiographic findings in an experimental rabbit model of congenital diaphragmatic hernia
Provedor de dados: 56
Autores: Manso,P.H.; Figueira,R.L.; Prado,C.M.; Gonçalves,F.L.; Simões,A.L.B.; Ramos,S.G.; Sbragia,L.
This study aimed to demonstrate that congenital diaphragmatic hernia (CDH) results in vascular abnormalities that are directly associated with the severity of pulmonary hypoplasia and hypertension. These events increase right ventricle (RV) afterload and may adversely affect disease management and patient survival. Our objective was to investigate cardiac function, specifically right ventricular changes, immediately after birth and relate them to myocardial histological findings in a CDH model. Pregnant New Zealand rabbits underwent the surgical procedure at 25 days of gestation (n=14). CDH was created in one fetus per horn (n=16), and the other fetuses were used as controls (n=20). At term (30 days), fetuses were removed, immediately dried and weighed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Congenital diaphragmatic hernia; Right ventricle; Pulmonary arterial hypertension; Echocardiography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300234

Staircase in mammalian muscle without light chain phosphorylation
Provedor de dados: 56
Autores: Rassier,D.E.; Tubman,L.A.; MacIntosh,B.R.
In disuse atrophied skeletal muscle, the staircase response is virtually absent and light chain phosphorylation does not occur. The purpose of the present study was to determine if staircase could be restored in atrophied muscle with continued absence of myosin light chain phosphorylation, by reducing what appears to be an otherwise enhanced calcium release. Control (untreated) and sham-operated female Sprague-Dawley rats were compared with animals after 2 weeks of complete inactivity induced by tetrodotoxin (TTX) application to the left sciatic nerve. In situ isometric contractile responses of rat gastrocnemius muscle were analyzed before and after administration of dantrolene sodium (DS), a drug which is known to inhibit Ca2+ release in skeletal muscle....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potentiation; Ca2+ availability; Tetrodotoxin (TTX); Atrophy; Myosin light chains.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100018

Psoriasis severity and hypothalamic-pituitary-adrenal axis function: results from the CALIPSO study
Provedor de dados: 56
Autores: Brunoni,A.R.; Santos,I.S.; Sabbag,C.; Lotufo,P.A.; Benseñor,I.M.
Psoriasis is a chronic inflammatory disease that significantly impacts life quality, being associated with stress and mental disorders. We investigated whether the activity of the hypothalamic-pituitary-adrenal (HPA) axis was associated with psoriasis severity, daily life stress and anxiety, and depressive symptoms. In this ancillary study, which was part of the CALIPSO (coronary artery calcium in psoriasis) study, saliva was collected from 102 patients with psoriasis immediately upon awakening, 30, and 60 min after awakening, at 2:00 pm and at bedtime (five time points) to determine salivary cortisol levels. We used Pearson's correlation coefficient to evaluate the association of clinical and psychopathological variables with HPA activity. We found a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psoriasis; Cortisol; HPA function; Clinical study.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201102

High levels of LDL-C combined with low levels of HDL-C further increase platelet activation in hypercholesterolemic patients
Provedor de dados: 56
Autores: Chan,L.W.; Luo,X.P.; Ni,H.C.; Shi,H.M.; Liu,L.; Wen,Z.C.; Gu,X.Y.; Qiao,J.; Li,J.
High levels of low-density lipoprotein cholesterol (LDL-C) enhance platelet activation, whereas high levels of high-density lipoprotein cholesterol (HDL-C) exert a cardioprotective effect. However, the effects on platelet activation of high levels of LDL-C combined with low levels of HDL-C (HLC) have not yet been reported. We aimed to evaluate the platelet activation marker of HLC patients and investigate the antiplatelet effect of atorvastatin on this population. Forty-eight patients with high levels of LDL-C were enrolled. Among these, 23 had HLC and the other 25 had high levels of LDL-C combined with normal levels of HDL-C (HNC). A total of 35 normocholesterolemic (NOMC) volunteers were included as controls. Whole blood flow cytometry and platelet...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Low-density lipoprotein cholesterol; High-density lipoprotein cholesterol; Platelet activation; Statins; PAC-1; CD62p.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200167

Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress
Provedor de dados: 56
Autores: Iizuka,Y.; Hong,S.; Kim,C.Y.; Yang,W.I.; Lee,J.E.; Seong,G.J.
Agmatine has neuroprotective effects on retinal ganglion cells (RGCs) as well as cortical and spinal neurons. It protects RGCs from oxidative stress even when it is not present at the time of injury. As agmatine has high affinity for various cellular receptors, we assessed protective mechanisms of agmatine using transformed RGCs (RGC-5 cell line). Differentiated RGC-5 cells were pretreated with 100 μM agmatine and consecutively exposed to 1.0 mM hydrogen peroxide (H2O2). Cell viability was determined by measuring lactate dehydrogenase (LDH), and the effects of selective alpha 2-adrenergic receptor antagonist yohimbine (0-500 nM) and N-methyl-D-aspartic acid (NMDA) receptor agonist NMDA (0-100 µM) were evaluated. Agmatine’s protective effect was compared to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Agmatine; Neuroprotection; Oxidative stress; Retinal ganglion cell.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400006

Endothelin-1 receptor antagonists protect the kidney against the nephrotoxicity induced by cyclosporine-A in normotensive and hypertensive rats
Provedor de dados: 56
Autores: Caires,A.; Fernandes,G.S.; Leme,A.M.; Castino,B.; Pessoa,E.A.; Fernandes,S.M.; Fonseca,C.D.; Vattimo,M.F.; Schor,N.; Borges,F.T.
Cyclosporin-A (CsA) is an immunosuppressant associated with acute kidney injury and chronic kidney disease. Nephrotoxicity associated with CsA involves the increase in afferent and efferent arteriole resistance, decreased renal blood flow (RBF) and glomerular filtration. The aim of this study was to evaluate the effect of Endothelin-1 (ET-1) receptor blockade with bosentan (BOS) and macitentan (MAC) antagonists on altered renal function induced by CsA in normotensive and hypertensive animals. Wistar and genetically hypertensive rats (SHR) were separated into control group, CsA group that received intraperitoneal injections of CsA (40 mg/kg) for 15 days, CsA+BOS and CsA+MAC that received CsA and BOS (5 mg/kg) or MAC (25 mg/kg) by gavage for 15 days. Plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetically hypertensive rats; Acute kidney injury; Cyclosporine-A; Bosentan; Macitentan; Endothelin-1.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200611

Arachidonic acid triggers an oxidative burst in leukocytes
Provedor de dados: 56
Autores: Pompeia,C.; Cury-Boaventura,M.F.; Curi,R.
The change in cellular reducing potential, most likely reflecting an oxidative burst, was investigated in arachidonic acid- (AA) stimulated leukocytes. The cells studied included the human leukemia cell lines HL-60 (undifferentiated and differentiated into macrophage-like and polymorphonuclear-like cells), Jurkat and Raji, and thymocytes and macrophages from rat primary cultures. The oxidative burst was assessed by nitroblue tetrazolium reduction. AA increased the oxidative burst until an optimum AA concentration was reached and the burst decreased thereafter. In the leukemia cell lines, optimum concentration ranged from 200 to 400 µM (up to 16-fold), whereas in rat cells it varied from 10 to 20 µM. Initial rates of superoxide generation were high,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachidonic acid; NADPH oxidase; Nitroblue tetrazolium; Leukocytes; Reactive oxygen species.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100013

Anti-hyperalgesic effect of electroacupuncture in a model of post-incisional pain in rats
Provedor de dados: 56
Autores: Oliveira,R.; Prado,W.A.
Electroacupuncture has been proposed to be a low cost and practical method that allows effective pain management with minimal collateral effects. In this study we have examined the effect of electroacupuncture against the hyperalgesia developed in a model of post-incisional pain in rats. A 1-cm longitudinal incision was made through the skin and fascia of the plantar region of the animal hind paw. Mechanical hyperalgesia in the incision was evaluated 135 min after the surgery with von Frey filaments. The tension threshold was reduced from 75 g (upper limit of the test) to 1.36 ± 0.36 g (mean ± SEM) in control rats. It is shown that a 15-min period of electroacupuncture applied 120 min after surgery to the Zusanli (ST36) and Sanyinjiao (SP6) points, but not...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Electroacupuncture; Post-incisional pain; Hyperalgesia; Naloxone.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800012

Axotomy increases NADPH-diaphorase activity in the dorsal root ganglia and lumbar spinal cord of the turtle Trachemys dorbigni
Provedor de dados: 56
Autores: Partata,W.A.; Krepsky,A.M.R.; Marques,M.; Achaval,M.
Seven days after transection of the sciatic nerve NADPH-diaphorase activity increased in the small and medium neurons of the dorsal root ganglia of the turtle. However, this increase was observed only in medium neurons for up to 90 days. At this time a bilateral increase of NADPH-diaphorase staining was observed in all areas and neuronal types of the dorsal horn, and in positive motoneurons in the lumbar spinal cord, ipsilateral to the lesion. A similar increase was also demonstrable in spinal glial and endothelial cells. These findings are discussed in relation to the role of nitric oxide in hyperalgesia and neuronal regeneration or degeneration.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NADPH-diaphorase; Axotomy; Turtle.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400018

Long-term correlation of the electrocorticogram as a bioindicator of brain exposure to ionizing radiation
Provedor de dados: 56
Autores: Aguiar,L.A.A.; Silva,I.M.S.; Fernandes,T.S.; Nogueira,R.A.
Understanding the effects of radiation and its possible influence on the nervous system are of great clinical interest. However, there have been few electrophysiological studies on brain activity after exposure to ionizing radiation (IR). A new methodological approach regarding the assessment of the possible effects of IR on brain activity is the use of linear and nonlinear mathematical methods in the analysis of complex time series, such as brain oscillations measured using the electrocorticogram (ECoG). The objective of this study was to use linear and nonlinear mathematical methods as biomarkers of gamma radiation regarding cortical electrical activity. Adult Wistar rats were divided into 3 groups: 1 control and 2 irradiated groups, evaluated at 24 h...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Power spectrum; Detrended fluctuation analysis; Electrocorticogram; Brain irradiation; Ionizing radiation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000009

Nitric oxide release from the S-nitrosothiol zinc phthalocyanine complex by flash photolysis
Provedor de dados: 56
Autores: Rotta,J.C.G.; Lunardi,C.N.; Tedesco,A.C.
The photogeneration of nitric oxide (NO) using laser flash photolysis was investigated for S-nitroso-glutathione (GSNO) and S-nitroso-N-acetylcysteine (NacySNO) at pH 6.4 (PBS/HCl) and 7.4 (PBS). Irradiation of S-nitrosothiol with light (lambda = 355 nm followed by absorption spectroscopy) resulted in the homolytic decomposition of NacySNO and GSNO to generate radicals (GS· and NacyS·) and NO. The release of NO from donor compounds measured with an ISO-Nometer apparatus was larger at pH 7.4 than pH 6.4. NacySNO was also incorporated into dipalmitoyl-phosphatidylcholine liposomes in the presence and absence of zinc phthalocyanine (ZnPC), a well-known photosensitizer useful for photodynamic therapy. Liposomes are usually used as carriers for hydrophobic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: S-nitrosothiols; Nitric oxide; Zinc phthalocyanine; Liposomes; Photodynamic therapy.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500005

Sertraline inhibits formalin-induced nociception and cardiovascular responses
Provedor de dados: 56
Autores: Santuzzi,C.H.; Futuro Neto,H.A.; Pires,J.G.P.; Gonçalves,W.L.S.; Tiradentes,R.V.; Gouvea,S.A.; Abreu,G.R.
The objective of the present study was to determine the antihyperalgesic effect of sertraline, measured indirectly by the changes of sciatic afferent nerve activity, and its effects on cardiorespiratory parameters, using the model of formalin-induced inflammatory nociception in anesthetized rats. Serum serotonin (5-HT) levels were measured in order to test their correlation with the analgesic effect. Male Wistar rats (250-300 g) were divided into 4 groups (N = 8/per group): sertraline-treated group (Sert + Saline (Sal) and Sert + Formalin (Form); 3 mg·kg-1·day-1, ip, for 7 days) and saline-treated group (Sal + Sal and Sal + Form). The rats were injected with 5% (50 µL) formalin or saline into the right hind paw. Sciatic nerve activity was recorded using a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sertraline; Formalin-induced pain; Antinociceptive effect; Cardiovascular system; Sciatic nerve activity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100008

Toluene permeabilization differentially affects F- and P-type ATPase activities present in the plasma membrane of Streptococcus mutans
Provedor de dados: 56
Autores: Thedei Jr.,G.; Leitão,D.P.S.; Bolean,M.; Paulino,T.P.; Spadaro,A.C.C.; Ciancaglini,P.
Streptococcus mutans membrane-bound P- and F-type ATPases are responsible for H+ extrusion from the cytoplasm thus keeping intracellular pH appropriate for cell metabolism. Toluene-permeabilized bacterial cells have long been used to study total membrane-bound ATPase activity, and to compare the properties of ATPase in situ with those in membrane-rich fractions. The aim of the present research was to determine if toluene permeabilization can significantly modify the activity of membrane-bound ATPase of both F-type and P-type. ATPase activity was assayed discontinuously by measuring phosphate release from ATP as substrate. Treatment of S. mutans membrane fractions with toluene reduced total ATPase activity by approximately 80% and did not allow...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell permeabilization; F-type ATPase; P-type ATPase; Toluene permeabilization; Streptococcus mutans.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200002

The effects of surgery on gastrointestinal motor activity
Provedor de dados: 56
Autores: Quigley,.M.M.; Thompson,J.S.
Gastrointestinal surgical procedures have the potential to disrupt motor activity in various organs of the gastrointestinal tract or, indeed, throughout the entire alimentary canal. Several of these motor effects have important clinical consequences and have also served to advance our understanding of the regulation of gastrointestinal motor activity. This review will focus, in particular, on the effects of surgery on the small intestine, and will attempt to emphasize the implications of these studies for our understanding of small intestinal motility, in general.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Motility; Motor activity; Small intestine; Surgery; Intestinal resection; Intestinal transplantation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700003

Novel mechanisms of growth hormone regulation: growth hormone-releasing peptides and ghrelin
Provedor de dados: 56
Autores: Lengyel,A.-M.J.
Growth hormone secretion is classically modulated by two hypothalamic hormones, growth hormone-releasing hormone and somatostatin. A third pathway was proposed in the last decade, which involves the growth hormone secretagogues. Ghrelin is a novel acylated peptide which is produced mainly by the stomach. It is also synthesized in the hypothalamus and is present in several other tissues. This endogenous growth hormone secretagogue was discovered by reverse pharmacology when a group of synthetic growth hormone-releasing compounds was initially produced, leading to the isolation of an orphan receptor and, finally, to its endogenous ligand. Ghrelin binds to an active receptor to increase growth hormone release and food intake. It is still not known how...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth hormone; Growth hormone secretagogues; Ghrelin; Somatostatin; Hypothalamus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800002

Oxygen uptake, respiratory exchange ratio, or total distance: a comparison of methods to equalize exercise volume in Wistar rats
Provedor de dados: 56
Autores: Paes,L.S.; Borges,J.P.; Cunha,F.A.; Souza,M.G.C.; Cyrino,F.Z.G.A.; Bottino,D.A.; Bouskela,E.; Farinatti,P.
This study compared strategies to equalize the volume of aerobic exercise performed with different intensities by Wistar rats, based on the distance covered during exercise bouts and energy expenditure (EE, isocaloric sessions) obtained from oxygen uptake (V̇O2) or respiratory exchange ratio (RER). Thirty-three male rats (270.5±12.8 g) underwent maximal exercise tests to determine V̇O2 reserve (V̇O2R), being randomly assigned to three groups: moderate-intensity continuous exercise at speed corresponding to 50% V̇O2R (MIC; n=11); high-intensity continuous exercise at 80% V̇O2R (HIC; n=11); and high-intensity intermittent exercise (HII; n=11) at 60% V̇O2R (3 min) and 80% V̇O2R (4 min). Exercise duration was calculated individually to elicit EE of 5 kcal in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Energy expenditure; Aerobic exercise; Exercise testing; Metabolism; Fitness.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800607

Looking for the GAP effect in manual responses and the role of contextual influences in reaction time experiments
Provedor de dados: 56
Autores: Faria Jr.,A.J.P.; Machado-Pinheiro,W.
When the offset of a visual stimulus (GAP condition) precedes the onset of a target, saccadic reaction times are reduced in relation to the condition with no offset (overlap condition) - the GAP effect. However, the existence of the GAP effect for manual responses is still controversial. In two experiments using both simple (Experiment 1, N = 18) and choice key-press procedures (Experiment 2, N = 12), we looked for the GAP effect in manual responses and investigated possible contextual influences on it. Participants were asked to respond to the imperative stimulus that would occur under different experimental contexts, created by varying the array of warning-stimulus intervals (0, 300 and 1000 ms) and conditions (GAP and overlap): i) intervals and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reaction times; Attention; Preparation; Expectancy; Contextual influences.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800007

Frequency of CCR5delta32 in Brazilian populations
Provedor de dados: 56
Autores: Vargas,A.E.; Marrero,A.R.; Salzano,F.M.; Bortolini,M.C.; Chies,J.A.B.
A sample of 103 randomly chosen healthy individuals from Alegrete, RS, Brazil, was tested for the CCR5delta32 allele, which is known to influence susceptibility to HIV-1 infection. The CCR5delta32 allele was identified by PCR amplification using specific primers flanking the region of deletion, followed by electrophoresis on a 3% agarose gel. The data obtained were compared to those reported for other populations and interpreted in terms of Brazilian history. The individuals studied came from a highly admixed population. Most of them were identified as white (N = 59), while blacks and browns (mulattoes) were N = 13 and N = 31, respectively. The observed frequencies, considering the white, black and brown samples (6.8, 3.8, and 6.4%, respectively), suggest...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CCR5; Chemokine receptors; Brazilian population; Gene flow.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300002

Transtracheal puncture: a forgotten procedure
Provedor de dados: 56
Autores: Almeida,E.P.; Almeida,A.C.; Almeida,F.F.; Montessi,J.; Gomes,C.A.; Ferreira,L.E.V.V.C.
Transtracheal puncture has long been known as a safe, low-cost procedure. However, with the advent of bronchoscopy, it has largely been forgotten. Two researchers have suggested the use of α-amylase activity to diagnose salivary aspiration, but the normal values of this enzyme in tracheobronchial secretions are unknown. We aimed to define the normal values of α-amylase activity in tracheobronchial secretions and verify the rate of major complications of transtracheal puncture. From October 2009 to June 2011, we prospectively evaluated 118 patients without clinical or radiological signs of salivary aspiration who underwent transtracheal puncture before bronchoscopy. The patients were sedated with a solution of lidocaine and diazepam until they reached a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transtracheal puncture; Aspiration; Complications; Technique; Amylase; Report values.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800725

Aspects of energetic substrate metabolism of in vitro and in vivo bovine embryos
Provedor de dados: 56
Autores: de Souza,D.K.; Salles,L.P.; Rosa e Silva,A.A.M.
Although the metabolism of early bovine embryos has not been fully elucidated, several publications have addressed this important issue to improve culture conditions for cattle reproductive biotechnologies, with the ultimate goal of producing in vitro embryos similar in quality to those developing in vivo. Here, we review general aspects of bovine embryo metabolism in vitro and in vivo, and discuss the use of metabolic analysis of embryos produced in vitro to assess viability and predict a viable pregnancy after transference to the female tract.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine pregnancy rates; Embryo metabolism; Metabolism profile; In vitro fertilization; Embryo culture conditions; In vivo embryo.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300191

Role of nitric oxide and prostaglandin in the maintenance of cortical and renal medullary blood flow
Provedor de dados: 56
Autores: Gomez,S.I; Strick,D.M.; Romero,J.C.
This study was undertaken in anesthetized dogs to evaluate the relative participation of prostaglandins (PGs) and nitric oxide (NO) in the maintenance of total renal blood flow (TRBF), and renal medullary blood flow (RMBF). It was hypothesized that the inhibition of NO should impair cortical and medullary circulation because of the synthesis of this compound in the endothelial cells of these two territories. In contrast, under normal conditions of perfusion pressure PG synthesis is confined to the renal medulla. Hence PG inhibition should predominantly impair the medullary circulation. The initial administration of 25 µM kg-1 min-1 NG-nitro-L-arginine methyl ester produced a significant 26% decrease in TRBF and a concomitant 34% fall in RMBF, while the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney; NG-nitro-L-arginine methyl ester; Meclofenamate; Renal circulation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200014

Measuring higher order optical aberrations of the human eye: techniques and applications
Provedor de dados: 56
Autores: Carvalho,L. Alberto V.; Castro,J.C.; Carvalho,L. Antonio V.
In the present paper we discuss the development of "wave-front", an instrument for determining the lower and higher optical aberrations of the human eye. We also discuss the advantages that such instrumentation and techniques might bring to the ophthalmology professional of the 21st century. By shining a small light spot on the retina of subjects and observing the light that is reflected back from within the eye, we are able to quantitatively determine the amount of lower order aberrations (astigmatism, myopia, hyperopia) and higher order aberrations (coma, spherical aberration, etc.). We have measured artificial eyes with calibrated ametropia ranging from +5 to -5 D, with and without 2 D astigmatism with axis at 45º and 90º. We used a device known as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Optical aberrations; Corneal topography; Zernike polynomials; Refractive surgery.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100019

Biophysical characteristics of gap junctions in vascular wall cells: implications for vascular biology and disease
Provedor de dados: 56
Autores: Brink,P.R.; Ricotta,J.; Christ,G.J.
The role gap junction channels play in the normal and abnormal functioning of the vascular wall is the subject of much research. The biophysical properties of gap junctions are an essential component in understanding how gap junctions function to allow coordinated relaxation and contraction of vascular smooth muscle. This study reviews the properties thus far elucidated and relates those properties to tissue function. We ask how biophysical and structural properties such as gating, permselectivity, subconductive states and channel type (heteromeric vs homotypic vs heterotypic) might affect vascular smooth muscle tone.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vascular smooth muscle; Gap junctions.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400007

Impairment of the hematological response and interleukin-1β production in protein-energy malnourished mice after endotoxemia with lipopolysaccharide
Provedor de dados: 56
Autores: Fock,R.A.; Vinolo,M.A.R.; Blatt,S.L.; Borelli,P.
The objectives of this study were to determine if protein-energy malnutrition (PEM) could affect the hematologic response to lipopolysaccharide (LPS), the interleukin-1β (IL-1β) production, leukocyte migration, and blood leukocyte expression of CD11a/CD18. Two-month-old male Swiss mice were submitted to PEM (N = 30) with a low-protein diet (14 days) containing 4% protein, compared to 20% protein in the control group (N = 30). The total cellularity of blood, bone marrow, spleen, and bronchoalveolar lavage evaluated after the LPS stimulus indicated reduced number of total cells in all compartments studied and different kinetics of migration in malnourished animals. The in vitro migration assay showed reduced capacity of migration after the LPS stimulus in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood; Bone marrow; Spleen; Interleukin-1β; Lipopolysaccharide; Malnutrition.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200009

Cardiovascular responses to microinjections of GABA or anesthetics into the rostral ventrolateral medulla of conscious and anesthetized rats
Provedor de dados: 56
Autores: Lacerda,J.E.C.; Campos,R.R.; Araujo,G.C.; Andreatta-Van Leyen,S.; Lopes,O.U.; Guertzenstein,P.G.
The rostral ventrolateral medulla (RVLM) contains neurons involved in tonic and reflex control of arterial pressure. We describe the effects of gamma-aminobutyric acid (GABA) and anesthetics injected into the RVLM of conscious and urethane (1.2 g/kg, iv) anesthetized Wistar rats (300-350 g). In conscious rats, bilateral microinjection of GABA (50 nmol/200 nl) induced a small but significant decrease in blood pressure (from 130 ± 3.6 to 110 ± 5.6 mmHg, N = 7). A similar response was observed with sodium pentobarbital microinjection (24 nmol/200 nl). However, in the same animals, the fall in blood pressure induced by GABA (from 121 ± 8.9 to 76 ± 8.8 mmHg, N = 7) or pentobarbital (from 118 ± 4.5 to 57 ± 11.3 mmHg, N = 6) was significantly increased after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vasomotor tone; Ventrolateral medulla; RVLM; GABA; Conscious rats.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900019

Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells
Provedor de dados: 56
Autores: Andrade,A.Q.; Casarini,D.E.; Schor,N.; Boim,M.A.
Renin is an enzyme involved in the stepwise generation of angiotensin II. Juxtaglomerular cells are the main source of plasma renin, but renin activity has been detected in other cell types. In the present study we evaluated the presence of renin mRNA in adult male Wistar rat and mouse (C-57 Black/6) mesangial cells (MC) and their ability to process, store and release both the active and inactive forms of the enzyme. Active renin and total renin content obtained after trypsin treatment were estimated by angiotensinogen consumption analyzed by SDS-PAGE electrophoresis and quantified by angiotensin I generation by HPLC. Renin mRNA, detected by RT-PCR, was present in both rat and mouse MC under basal conditions. Active renin was significantly higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin; Prorenin; Mesangial cell; Kidney.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100003

Propofol inhibits proliferation, migration, and invasion but promotes apoptosis by regulation of Sox4 in endometrial cancer cells
Provedor de dados: 56
Autores: Du,Qing; Liu,Jia; Zhang,Xuezhi; Zhang,Xin; Zhu,He; Wei,Ming; Wang,Shilei.
Propofol is an intravenous sedative hypnotic agent of which the growth-inhibitory effect has been reported on various cancers. However, the roles of propofol in endometrial cancer (EC) remain unclear. This study aimed to explore the effects of propofol on EC in vitro and in vivo. Different concentrations of propofol were used to treat Ishikawa cells. Colony number, cell viability, cell cycle, apoptosis, migration, and invasion were analyzed by colony formation, MTT, flow cytometry, and Transwell assays. In addition, the pcDNA3.1-Sox4 and Sox4 siRNA plasmids were transfected into Ishikawa cells to explore the relationship between propofol and Sox4 in EC cell proliferation. Tumor weight in vivo was measured by xenograft tumor model assay. Protein levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endometrial cancer; Propofol; Cell proliferation; Apoptosis; Sox4; Wnt/β-catenin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400605

Urinary iron excretion induced by intravenous infusion of deferoxamine in ß-thalassemia homozygous patients
Provedor de dados: 56
Autores: Boturão-Neto,E.; Marcopito,L.F.; Zago,M.A.
The purpose of the present study was to identify noninvasive methods to evaluate the severity of iron overload in transfusion-dependent ß-thalassemia and the efficiency of intensive intravenous therapy as an additional tool for the treatment of iron-overloaded patients. Iron overload was evaluated for 26 ß-thalassemia homozygous patients, and 14 of them were submitted to intensive chelation therapy with high doses of intravenous deferoxamine (DF). Patients were classified into six groups of increasing clinical severity and were divided into compliant and non-compliant patients depending on their adherence to chronic chelation treatment. Several methods were used as indicators of iron overload. Total gain of transfusion iron, plasma ferritin, and urinary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SS-Thalassemia; Iron overload; Deferoxamine; Iron excretion.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100009

Editorial
Provedor de dados: 56
Autores: Teixeira,M.M.; Cunha,F.Q.; Ferreira,S.H.
Tipo: Info:eu-repo/semantics/other
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500001

Evaluation of anti-Wnt/β-catenin signaling agents by pGL4-TOP transfected stable cells with a luciferase reporter system
Provedor de dados: 56
Autores: Chuang,K.A.; Lieu,C.H.; Tsai,W.J.; Wu,M.H.; Chen,Y.C.; Liao,J.F.; Wang,C.C.; Kuo,Y.C.
Refractory and relapsed leukemia is a major problem during cancer therapy, which is due to the aberrant activation of Wnt/β-catenin signaling pathway. Activation of this pathway is promoted by wingless (Wnt) proteins and induces co-activator β-catenin binding to lymphoid enhancer factor (LEF)/T-cell factor protein (TCF). To provide a convenient system for the screening of anti-Wnt/β-catenin agents, we designed a bi-functional pGL4-TOP reporter plasmid that contained 3X β-catenin/LEF/TCF binding sites and a selectable marker. After transfection and hygromycin B selection, HEK 293-TOP and Jurkat-TOP stable clones were established. The luciferase activity in the stable clone was enhanced by the recombinant Wnt-3A (rWnt-3A; 100-400 ng/mL) and GSK3β inhibitor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Plasmids; Reporter assay; Screening; Wnt; Β-catenin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000003

The availability of full match sibling donors and feasibility of allogeneic bone marrow transplantation in Brazil
Provedor de dados: 56
Autores: Eid,K.A.B.; Miranda,E.C.M.; Vigorito,A.C.; Aranha,F.J.P.; Oliveira,G.B.; De Souza,C.A.
The feasibility of allogeneic bone marrow transplantation (alloBMT) in a developing country has not yet been demonstrated. Many adverse factors including social and economic limitations may reduce the overall results of this complex and expensive procedure. Our objective was to characterize the most important clinical, social and economic features of candidates for transplantation and their potential donors as well as the influence of these factors on overall survival in a retrospective and exploratory analysis at a university hospital. From July 1993 to July 2001, candidates for BMT were referred to the Bone Marrow Transplantation Unit by Hematology and Oncology Centers from several regions of Brazil. A total of 1138 patients were referred to us as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allogeneic bone marrow transplantation; Selection of donors; Social and economic conditions.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300005

Inflammatory response of the spinal cord to multiple episodes of blood-brain barrier disruption and toxic demyelination in Wistar rats
Provedor de dados: 56
Autores: Fernandes,C.G.; Graça,D.L.; Pereira,L.A.V.D.
Multiple episodes of blood-brain barrier disruption were induced by sequential intraspinal injections of ethidium bromide. In addition to the barrier disruption, there was toxic demyelination and exposure of myelin components to the immune system. Twenty-seven 3-month-old Wistar rats received 2, 3 or 4 injections of 1 µl of either 0.1% ethidium bromide in normal saline (19 rats) or 0.9% saline (8 rats) at different levels of the spinal cord. The time intervals between the injections ranged from 28 to 42 days. Ten days after the last injection, all rats were perfused with 2.5% glutaraldehyde. The spinal sections were evaluated macroscopically and by light and transmission electron microscopy. All the lesions demonstrated a mononuclear phagocytic infiltrate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphocytes; Multiple sclerosis; Central nervous system; Blood-brain barrier; Ethidium bromide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700008

Antidepressant effects of Kai-Xin-San in fluoxetine-resistant depression rats
Provedor de dados: 56
Autores: Dong,X.Z.; Wang,D.X.; Lu,Y.P.; Yuan,S.; Liu,P.; Hu,Y.
This study aimed to investigate the antidepressant effect and the mechanism of action of Kai-Xin-San (KXS) in fluoxetine-resistant depressive (FRD) rats. Two hundred male Wistar rats weighing 200±10 g were exposed to chronic and unpredictable mild stresses (CUMS) for 4 weeks and given fluoxetine treatment simultaneously. The rats that did not show significant improvement in behavioral indexes were chosen as the FRD model rats. These rats were randomly divided into four groups: FRD model control; oral fluoxetine and aspirin; oral KXS at a dose of 338 mg·kg–1·day–1; and oral KXS at a dose of 676 mg·kg–1·day–1. Rats continued to be exposed to CUMS and underwent treatment once a day for 3 weeks, then cytokine (COX-2, IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-10,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kai-Xin-San; Fluoxetine-resistant depression; Chronic unpredictable mild stress; Inflammatory factor; Anti-depression.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000601

Antiphospholipid antibodies in Brazilian hepatitis C virus carriers
Provedor de dados: 56
Autores: Atta,A.M.; Estevam,P.; Paraná,R.; Pereira,C.M.; Leite,B.C.O.; Sousa-Atta,M.L.B.
Hepatitis C, a worldwide viral infection, is an important health problem in Brazil. The virus causes chronic infection, provoking B lymphocyte dysfunction, as represented by cryoglobulinemia, non-organ-specific autoantibody production, and non-Hodgkin's lymphoma. The aim of this research was to screen for the presence of antiphospholipid autoantibodies in 109 Brazilian hepatitis C virus carriers without clinical history of antiphospholipid syndrome. Forty healthy individuals were used as the control group. IgA, IgG, and IgM antibodies against cardiolipin and β2-glycoprotein I were measured with an enzyme-linked immunosorbent assay, using a cut-off point of either 20 UPL or 20 SBU. While 24 (22.0%) hepatitis C carriers had moderate titers of IgM...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis C virus; Antiphospholipid antibody; Brazilian patients.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600009

A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry
Provedor de dados: 56
Autores: Alves,L.P.S.; Almeida,A.T.; Cruz,L.M.; Pedrosa,F.O.; de Souza,E.M.; Chubatsu,L.S.; Müller-Santos,M.; Valdameri,G.
The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) production by the diazotrophic and plant-associated bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. The method consists of three steps: i) cell permeabilization, ii) Nile red staining, and iii) analysis by flow cytometry. The method was optimized step-by-step and can be carried out in less than 5 min. The final results indicated a high correlation coefficient (R2=0.99) compared to a standard method based on methanolysis and GC. This method...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flow cytometry; Nile red; Poly-3-hydroxybutyrate; Herbaspirillum seropedicae; Azospirillum brasilense.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100606

Prevalence of the STK15 F31I polymorphism and its relationship with mammographic density
Provedor de dados: 56
Autores: Giacomazzi,J.; Aguiar,E.; Palmero,E.I.; Schmidt,A.V.; Skonieski,G.; Duarte Filho,D.; Bock,H.; Saraiva-Pereira,M.L.; Schuler-Faccini,L.; Camey,S.A.; Caleffi,M.; Giugliani,R.; Ashton-Prolla,P.
Several studies have identified the single nucleotide polymorphism STK15 F31I as a low-penetrance risk allele for breast cancer, but its prevalence and risk association in the Brazilian population have not been determined. The goal of this study was to identify the frequency of this polymorphism in the Brazilian setting. Considering the high degree of admixture of our population, it is of fundamental importance to validate the results already reported in the literature and also to verify the relationship between this variant and breast cancer risk. A total of 750 women without breast cancer were genotyped using the TaqMan PCR assay for STK15 F31I polymorphism. Clinical information was obtained from review of the medical records and mammographic density...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: STK15 F31I polymorphism; Breast cancer risk; Mammographic density.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400004

Respiratory mechanics and morphometric changes during anesthesia with ketamine in normal rats
Provedor de dados: 56
Autores: Alves-Neto,O.; Tavares,P.; Rocco,P.R.M.; Zin,W.A.
Ketamine is believed to reduce airway and pulmonary tissue resistance. The aim of the present study was to determine the effects of ketamine on the resistive, elastic and viscoelastic/inhomogeneous mechanical properties of the respiratory system, lungs and chest wall, and to relate the mechanical data to findings from histological lung analysis in normal animals. Fifteen adult male Wistar rats were assigned randomly to two groups: control (N = 7) and ketamine (N = 8). All animals were sedated (diazepam, 5 mg, ip) and anesthetized with pentobarbital sodium (20 mg/kg, ip) or ketamine (30 mg/kg, ip). The rats were paralyzed and ventilated mechanically. Ketamine increased lung viscoelastic/inhomogeneous pressure (26%) compared to the control group. Dynamic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ketamine; Elastance; Lung tissue; Resistive pressure; Viscoelasticity; Rat.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900016

Hepatitis B virus genotype E detected in Brazil in an African patient who is a frequent traveler
Provedor de dados: 56
Autores: Sitnik,R; Sette Jr,H; Santana,R.A.F; Menezes,L.C; Graça,C.H.N; Dastoli,G.T.F; Silbert,S; Pinho,J.R.R.
Genotype E of hepatitis B virus (HBV) has not been described in Brazil and is found mainly in Africa. Genotype A is the most prevalent in Brazil, and genotypes B, C, D, and F have already been reported. We report here an HBV genotype E-infected patient and some characterization of surface (S) protein, DNA polymerase (P) and precore/core (preC/C) coding regions based on the viral genome. The patient is a 31-year-old black man with chronic hepatitis B who was born and raised in Angola. He has been followed by a hepatologist in São Paulo, Brazil, since November 2003, and he is a frequent traveler to Latin America, Africa, and Europe. In 2003, he was diagnosed with HBV infection and started treatment with lamivudine with the later addition of adefovir...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis B; HBV genotype E; Precore mutation; Brazil; Africa; African traveler.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200013

Brazilian version of the Berg balance scale
Provedor de dados: 56
Autores: Miyamoto,S.T.; Lombardi Junior,I.; Berg,K.O.; Ramos,L.R.; Natour,J.
The purpose of the present study was to translate and adapt the Berg balance scale, an instrument for functional balance assessment, to Brazilian-Portuguese and to determine the reliability of scores obtained with the Brazilian adaptation. Two persons proficient in English independently translated the original scale into Brazilian-Portuguese and a consensus version was generated. Two translators performed a back translation. Discrepancies were discussed and solved by a panel. Forty patients older than 65 years and 40 therapists were included in the cultural adaptation phase. If more than 15% of therapists or patients reported difficulty in understanding an item, that item was reformulated and reapplied. The final Brazilian version was then tested on 36...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Balance; Outcome assessment; Disability evaluation; Rehabilitation; Geriatric patients; Postural control.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900017

AKT inhibitor suppresses hyperthermia-induced Ndrg2 phosphorylation in gastric cancer cells
Provedor de dados: 56
Autores: Tao,Yurong; Guo,Yan; Liu,Wenchao; Zhang,Jian; Li,Xia; Shen,Lan; Ru,Yi; Xue,Yan; Zheng,Jin; Liu,Xinping; Zhang,Jing; Yao,Libo.
Hyperthermia is one of the most effective adjuvant treatments for various cancers with few side effects. However, the underlying molecular mechanisms still are not known. N-myc downstream-regulated gene 2 (NDRG2), a tumor suppressor, has been shown to be involved in diverse cellular stresses including hypoxia, lipotoxicity, etc. In addition, Ndrg2 has been reported to be related to progression of gastric cancer. In the current study, our data showed that the apoptosis rate of MKN28 cells increased relatively rapidly to 13.4% by 24 h after treatment with hyperthermia (42°C for 1 h) compared to 5.1% in control cells (P &lt; 0.05). Nevertheless, there was no obvious change in the expression level of total Ndrg2 during this process. Further investigation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperthermia; Gastric cancer; Ndrg2; Apoptosis; AKT inhibitor.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000400394

Cantharidin suppressed breast cancer MDA-MB-231 cell growth and migration by inhibiting MAPK signaling pathway
Provedor de dados: 56
Autores: Gu,X.-D.; Xu,L.-L; Zhao,H.; Gu,J.-Z; Xie,X.-H.
As an active constituent of the beetle Mylabris used in traditional Chinese medicine, cantharidin is a potent and selective inhibitor of protein phosphatase 2A (PP2A) that plays a crucial role in cell cycle progression, apoptosis, and cell fate. The role and possible mechanisms exerted by cantharidin in cell growth and metastasis of breast cancer were investigated in this study. Cantharidin was found to inhibit cell viability and clonogenic potential in a time- and dose-dependent manner. Cell cycle analysis revealed that cell percentage in G2/M phase decreased, whereas cells in S and G1 phases progressively accumulated with the increasing doses of cantharidin treatment. In a xenograft model of breast cancer, cantharidin inhibited tumor growth in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cantharidin; Breast cancer; ERK/MAPK pathway; Growth; Migration; Invasion.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700608

CCAAT/enhancer-binding protein alpha (CEBPA) gene haploinsufficiency does not alter hematopoiesis or induce leukemia in Lck-CALM/AF10 transgenic mice
Provedor de dados: 56
Autores: Lange,A.P.; Almeida,L.Y.; Araújo Silva,C.L.; Scheucher,P.S.; Chahud,F.; Krause,A.; Bohlander,S.K.; Rego,E.M.
Although rare, CALM/AF10 is a chromosomal rearrangement found in immature T-cell acute lymphoblastic leukemia (T-ALL), acute myeloid leukemia, and mixed phenotype acute leukemia of T/myeloid lineages with poor prognosis. Moreover, this translocation is detected in 50% of T-ALL patients with gamma/delta T cell receptor rearrangement, frequently associated with low expression of transcription factor CCAAT/enhancer-binding protein alpha (CEBPA). However, the relevance of CEBPA low expression for CALM/AF10 leukemogenesis has not yet been evaluated. We generated double mutant mice, which express the Lck-CALM/AF10 fusion gene and are haploinsufficient for the Cebpa gene. To characterize the hematopoiesis, we quantified hematopoietic stem cells, myeloid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukemia; CEBPA; CALM/AF10; Transgenic mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600604

Total and segmental colonic transit time in constipated patients with Chagas’ disease without megaesophagus or megacolon
Provedor de dados: 56
Autores: Santos,S.L.; Barcelos,I.K.; Mesquita,M.A.
Manometric and pharmacological tests have shown that motor abnormalities may occur in the non-dilated colons of chagasic patients. In order to investigate the presence of abnormalities of colonic function in constipated patients with Chagas’ disease (ChC) without megaesophagus or megacolon, studies of total and segmental colonic transit time with radiopaque markers were performed on 15 ChC patients, 27 healthy volunteers and 17 patients with idiopathic constipation (IC). The values obtained for the control group were similar to those reported in the literature (total colonic time: 34.1 ± 15.6 h; right colon: 9.9 ± 7.3 h; left colon: 10.8 ± 10 h, and rectosigmoid: 12.6 ± 9.9 h). Colonic transit time data permitted us to divide both IC and ChC patients into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colonic transit; Chagas’ disease; Constipation; Radiopaque markers; American trypanosomiasis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100006

Randomized controlled trials of serotonin-norepinephrine reuptake inhibitor in treating major depressive disorder in children and adolescents: a meta-analysis of efficacy and acceptability
Provedor de dados: 56
Autores: Xu,Y.; Bai,S.J.; Lan,X.H.; Qin,B.; Huang,T.; Xie,P.
New generation antidepressant therapies, including serotonin-norepinephrine reuptake inhibitor (SNRIs), were introduced in the late 1980s; however, few comprehensive studies have compared the benefits and risks of various contemporary treatments for major depressive disorder (MDD) in young patients. A comprehensive literature search of PubMed, Cochrane, Embase, Web of Science, and PsycINFO databases was conducted from 1970 to January 2015. Only clinical trials that randomly assigned one SNRI or placebo to patients aged 7 to 18 years who met the diagnostic criteria for major depressive disorder were included. Treatment success, dropout rate, and suicidal ideation/attempt outcomes were measured. Primary efficacy was determined by pooling the risk ratios...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adolescent; Child; Major depressive disorder; Meta-analysis; Randomized controlled trial; Serotonin and norepinephrine reuptake inhibitor.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600704

Pattern of functional antibody activity against Haemophilus influenzae type b (Hib) in infants immunized with diphtheria-tetanus-pertussis/Hib Brazilian combination vaccine
Provedor de dados: 56
Autores: Matos,D.C.S.; Silva,A.M.V.; Neves,P.C.C.; Martins,R.M.; Homma,A.; Marcovistz,R.
We evaluated the functional activity of Haemophilus influenzae B (Hib) antibodies elicited in a group of infants immunized with the diphtheria-tetanus-pertussis vaccine combined with an Hib vaccine produced totally in Brazil after technological transfer of Hib vaccine production from Glaxo SmithKline, Belgium. Blood samples from immunized infants (N = 985) were collected for the determination of Hib antibodies. Total Ig and IgM and IgG subclasses of antibodies against polyribosyl ribitol phosphate (PRP) were analyzed by ELISA. Almost all vaccinees (97.56%, 961/985) developed a strong anti-PRP IgG antibody response (≥1.0 μg/mL), while an anti-PRP IgM response was observed in 64.24% (634/985) of them (≥0.15 μg/mL). Only 18.88% (186/985) of the infants in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DTP-Hib vaccine; Haemophilus influenzae type b; Antibody avidity; PRP immune response.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200020

Recombinant vaccines and the development of new vaccine strategies
Provedor de dados: 56
Autores: Nascimento,I.P.; Leite,L.C.C.
Vaccines were initially developed on an empirical basis, relying mostly on attenuation or inactivation of pathogens. Advances in immunology, molecular biology, biochemistry, genomics, and proteomics have added new perspectives to the vaccinology field. The use of recombinant proteins allows the targeting of immune responses focused against few protective antigens. There are a variety of expression systems with different advantages, allowing the production of large quantities of proteins depending on the required characteristics. Live recombinant bacteria or viral vectors effectively stimulate the immune system as in natural infections and have intrinsic adjuvant properties. DNA vaccines, which consist of non-replicating plasmids, can induce strong...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human vaccines; Vectors for immunization; Safety; Recombinant vaccines.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200001

Changes in the biogenic amine content of the prefrontal cortex, amygdala, dorsal hippocampus, and nucleus accumbens of rats submitted to single and repeated sessions of the elevated plus-maze test
Provedor de dados: 56
Autores: Carvalho,M.C.; Albrechet-Souza,L.; Masson,S.; Brandão,M.L.
It has been demonstrated that exposure to a variety of stressful experiences enhances fearful reactions when behavior is tested in current animal models of anxiety. Until now, no study has examined the neurochemical changes during the test and retest sessions of rats submitted to the elevated plus maze (EPM). The present study uses a new approach (HPLC) by looking at the changes in dopamine and serotonin levels in the prefrontal cortex, amygdala, dorsal hippocampus, and nucleus accumbens in animals upon single or double exposure to the EPM (one-trial tolerance). The study involved two experiments: i) saline or midazolam (0.5 mg/kg) before the first trial, and ii) saline or midazolam before the second trial. For the biochemical analysis a control group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: One-trial tolerance; Dopamine; Serotonin; Prefrontal cortex; Amygdala; Hippocampus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200014

Individual variation is the key to the development of a vaccine against Staphylococcus aureus: a comparative study between mice lineages
Provedor de dados: 56
Autores: dos Santos,D.P.; Muniz,I.P.R.; Queiroz,A.F.; Pereira,I.S.; Souza,M.P.A.; Lima,L.J.; Sousa,L.R.O.; Ribeiro,I.S.; Galantini,M.P.L.; Marques,L.M.; Figueiredo,T.B.; da Silva,R.A.A.
Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. aureus infection in intradermally immunized mice. C57BL/6 and A/j mice were immunized intradermally with S. aureus inactivated by heat and then challenged with viable strains in an air pouch model. At 6, 12, and 24 h after the challenge, euthanasia was performed, and the cellular profile of the inflammatory infiltrate, cytokines, and the bacterial load were evaluated in the air pouch lavages. Immunized mice demonstrated that the intradermal immunization with S. aureus promoted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunization; Staphylococcus aureus; Mice; Air pouch; Antibodies.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500616

Effect of trolox C on cardiac contracture induced by hydrogen peroxide
Provedor de dados: 56
Autores: Belló-Klein,A.; Oliveira,A.R.; Miranda,M.F.S.; Irigoyen,M.C.; Homem-de-Bittencourt Jr.,P.I.; Llesuy,S.; Belló,A.A.
Hydrogen peroxide (H2O2) perfused into the aorta of the isolated rat heart induces a positive inotropic effect, with cardiac arrhythmia such as extrasystolic potentiation or cardiac contractures, depending on the dose. The last effect is similar to the "stone heart" observed in reperfusion injury and may be ascribed to lipoperoxidation (LPO) of the membrane lipids, to protein damage, to reduction of the ATP level, to enzymatic alterations and to cardioactive compounds liberated by LPO. These effects may result in calcium overload of the cardiac fibers and contracture ("stone heart"). Hearts from male Wistar rats (300-350 g) were perfused at 31oC with Tyrode, 0.2 mM trolox C, 256 mM H2O2 or trolox C + H2O2. Cardiac contractures (baseline elevation of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trolox C; "stone heart"; Free radicals; Hydrogen peroxide.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100013

Proteomic inventory of myocardial proteins from patients with chronic Chagas' cardiomyopathy
Provedor de dados: 56
Autores: Teixeira,P.C.; Iwai,L.K.; Kuramoto,A.C.K.; Honorato,R.; Fiorelli,A.; Stolf,N.; Kalil,J.; Cunha-Neto,E.
Chronic Chagas' disease cardiomyopathy (CCC) is an often fatal outcome of Trypanosoma cruzi infection, with a poorer prognosis than other cardiomyopathies. CCC is refractory to heart failure treatments, and is the major indication of heart transplantation in Latin America. A diffuse myocarditis, plus intense myocardial hypertrophy, damage and fibrosis, in the presence of very few T. cruzi forms, are the histopathological hallmarks of CCC. To gain a better understanding of the pathophysiology of CCC, we analyzed the protein profile in the affected CCC myocardium. Homogenates from left ventricular myocardial samples of end-stage CCC hearts explanted during heart transplantation were subjected to two-dimensional electrophoresis with Coomassie blue staining;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chagas' disease; Cardiomyopathy; Proteomic analysis; Two-dimensional electrophoresis; MALDI-ToF.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200005

Development of new heparin-like compounds and other antithrombotic drugs and their interaction with vascular endothelial cells
Provedor de dados: 56
Autores: Nader,H.B.; Pinhal,M.A.S.; Baú,E.C.; Castro,R.A.B.; Medeiros,G.F.; Chavante,S.F.; Leite,E.L.; Trindade,E.S.; Shinjo,S.K.; Rocha,H.A.O.; Tersariol,I.L.S.; Mendes,A.; Dietrich,C.P.
The anticlotting and antithrombotic activities of heparin, heparan sulfate, low molecular weight heparins, heparin and heparin-like compounds from various sources used in clinical practice or under development are briefly reviewed. Heparin isolated from shrimp mimics the pharmacological activities of low molecular weight heparins. A heparan sulfate from Artemia franciscana and a dermatan sulfate from tuna fish show a potent heparin cofactor II activity. A heparan sulfate derived from bovine pancreas has a potent antithrombotic activity in an arterial and venous thrombosis model with a negligible activity upon the serine proteases of the coagulation cascade. It is suggested that the antithrombotic activity of heparin and other antithrombotic agents is due...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heparin; Anticlotting activity heparan sulfate; Antithrombotic activity low molecular weight heparins; Preparation and antithrombotic activity antithrombotic agents; Development for clinical use crustacean heparin-like compounds; Antithrombotic activity antithrombotic agents vascular endothelial cells.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600002

Gadolinium increases the vascular reactivity of rat aortic rings
Provedor de dados: 56
Autores: Angeli,J.K.; Ramos,D.B.; Casali,E.A.; Souza,D.O.G.; Sarkis,J.J.F.; Stefanon,I.; Vassallo,D.V.; Fürstenau,C.R.
Gadolinium (Gd) blocks intra- and extracellular ATP hydrolysis. We determined whether Gd affects vascular reactivity to contractile responses to phenylephrine (PHE) by blocking aortic ectonucleoside triphosphate diphosphohydrolase (E-NTPDase). Wistar rats of both sexes (260-300 g, 23 females, 7 males) were used. Experiments were performed before and after incubation of aortic rings with 3 µM Gd. Concentration-response curves to PHE (0.1 nM to 0.1 mM) were obtained in the presence and absence of endothelium, after incubation with 100 µM L-NAME, 10 µM losartan, or 10 µM enalaprilat. Gd significantly increased the maximum response (control: 72.3 ± 3.5; Gd: 101.3 ± 6.4%) and sensitivity (control: 6.6 ± 0.1; Gd: 10.5 ± 2.8%) to PHE. To investigate the blockade...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gadolinium; E-NTPDase; Adenosine; Angiotensin II; AT1receptor.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500010

Anti-placental growth factor antibody ameliorates hyperoxia-mediated impairment of lung development in neonatal rats
Provedor de dados: 56
Autores: Zhang,Zhiqun; Zhong,Ying; Li,Xiaoxia; Huang,Xianmei; Du,Lizhong.
This study investigates the effect of the overexpression of the placental growth factor (PGF) and hyperoxia on lung development and determines whether anti-PGF antibody ameliorates hyperoxia-mediated impairment of lung development in newborn rats. After exposure to normoxic conditions for seven days, newborn rats subjected to normoxia were intraperitoneally or intratracheally injected with physiological saline, adenovirus-negative control (Ad-NC), or adenovirus-PGF (Ad-PGF) to create the Normoxia, Normoxia+Ad-NC, and Normoxia+Ad-PGF groups, respectively. Newborn rats subjected to hyperoxia were intraperitoneally injected with physiological saline or anti-PGF antibodies to create the Hyperoxia and Hyperoxia+anti-PGF groups, respectively. Our results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bronchopulmonary dysplasia; Anti-PGF antibody; Newborn rats; Hyperoxia; Lung tissue injury.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200604

Low-intensity red and infrared laser effects at high fluences on Escherichia coli cultures
Provedor de dados: 56
Autores: Barboza,L.L.; Campos,V.M.A.; Magalhães,L.A.G.; Paoli,F.; Fonseca,A.S.
Semiconductor laser devices are readily available and practical radiation sources providing wavelength tenability and high monochromaticity. Low-intensity red and near-infrared lasers are considered safe for use in clinical applications. However, adverse effects can occur via free radical generation, and the biological effects of these lasers from unusually high fluences or high doses have not yet been evaluated. Here, we evaluated the survival, filamentation induction and morphology of Escherichia coli cells deficient in repair of oxidative DNA lesions when exposed to low-intensity red and infrared lasers at unusually high fluences. Cultures of wild-type (AB1157), endonuclease III-deficient (JW1625-1), and endonuclease IV-deficient (JW2146-1) E. coli, in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Escherichia coli; Filamentation; Laser.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000013

Electron paramagnetic resonance study of lipid and protein membrane components of erythrocytes oxidized with hydrogen peroxide
Provedor de dados: 56
Autores: Mendanha,S.A.; Anjos,J.L.V.; Silva,A.H.M.; Alonso,A.
Electron paramagnetic resonance (EPR) spectroscopy of spin labels was used to monitor membrane dynamic changes in erythrocytes subjected to oxidative stress with hydrogen peroxide (H2O2). The lipid spin label, 5-doxyl stearic acid, responded to dramatic reductions in membrane fluidity, which was correlated with increases in the protein content of the membrane. Membrane rigidity, associated with the binding of hemoglobin (Hb) to the erythrocyte membrane, was also indicated by a spin-labeled maleimide, 5-MSL, covalently bound to the sulfhydryl groups of membrane proteins. At 2% hematocrit, these alterations in membrane occurred at very low concentrations of H2O2 (50 µM) after only 5 min of incubation at 37°C in azide phosphate buffer, pH 7.4. Lipid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electron paramagnetic resonance; Spin label; Erythrocyte; Membrane fluidity; Membrane oxidation; Protein oxidation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600001

Diurnal variation of vascular diameter and reactivity in healthy young men
Provedor de dados: 56
Autores: Bau,P.F.D.; Bau,C.H.D.; Naujorks,A.A.; Rosito,G.A.; Fuchs,F.D.
The higher incidence of cardiovascular events in the morning is accompanied by an increased vascular tone. However, there are few published studies designed to evaluate the diurnal variation of vascular and endothelial parameters in healthy subjects. In the present investigation, we evaluated the diurnal variation in brachial artery diameter (BAD), flow-mediated dilation (FMD) and endothelium-independent dilation (NFMD) in a homogeneous sample of healthy non-smoker young men. Fifty subjects aged 20.8 ± 0.3 years (range: 18 to 25 years) were investigated by brachial artery ultrasound. Exclusion criteria were female gender and evidence of clinically significant health problems, including obesity. Volunteers were asked to rest and avoid fat meals as well as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular risk factor; Circadian rhythm; Brachial artery; Vasoconstriction; Vascular biology.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600011

Vascular dysfunction by myofibroblast activation in patients with idiopathic pulmonary fibrosis and prognostic significance
Provedor de dados: 56
Autores: Parra,E.R.; Falzoni,R.; Capelozzi,V.L.
In this study, we demonstrated the importance of telomerase protein expression and determined the relationships among telomerase, endothelin-1 (ET-1) and myofibroblasts during early and late remodeling of parenchymal and vascular areas in usual interstitial pneumonia (UIP) using 27 surgical lung biopsies from patients with idiopathic pulmonary fibrosis (IPF). Telomerase+, myofibroblasts α-SMA+, smooth muscle cells caldesmon+, endothelium ET-1+ cellularity, and fibrosis severity were evaluated in 30 fields covering normal lung parenchyma, minimal fibrosis (fibroblastic foci), severe (mural) fibrosis, and vascular areas of UIP by the point-counting technique and a semiquantitative score. The impact of these markers was determined in pulmonary functional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Idiopathic pulmonary fibrosis; Vascular activity; Immunohistochemistry; Survival.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700015

Low prevalence of relevant findings in inappropriate echocardiograms and discordant perceptions between cardiologists and patients
Provedor de dados: 56
Autores: Lopes,J.R.; Oliveira,A.C.; Rios,V.G.; Correia,L.C.L.
Complementary examinations are “inadequate” whenever the likelihood of benefits from their indication is lower than the negative results. The low benefit is a result of poor performance in detecting relevant changes that lead to improved behavior. However, inadequate examinations are prevalent and little is known about patients' notions of the usefulness of such indications. The aim of this study was to describe relevant findings in inappropriate echocardiograms and to assess the level of agreement between patients and cardiologists regarding their usefulness. Adults without known cardiovascular disease who were referred for echocardiogram by inappropriate criteria according to the American College of Cardiology were selected. Relevant findings were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Echocardiogram; Inappropriate; Utility; Patients; Cardiologists.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800606

Effects of exercise training on atrophy gene expression in skeletal muscle of mice with chronic allergic lung inflammation
Provedor de dados: 56
Autores: Durigan,J.L.Q.; Peviani,S.M.; Russo,T.L.; Silva,A.C.D.; Vieira,R.P.; Martins,M.A.; Carvalho,C.R.F.; Salvini,T.F.
We evaluated the effects of chronic allergic airway inflammation and of treadmill training (12 weeks) of low and moderate intensity on muscle fiber cross-sectional area and mRNA levels of atrogin-1 and MuRF1 in the mouse tibialis anterior muscle. Six 4-month-old male BALB/c mice (28.5 ± 0.8 g) per group were examined: 1) control, non-sensitized and non-trained (C); 2) ovalbumin sensitized (OA, 20 µg per mouse); 3) non-sensitized and trained at 50% maximum speed _ low intensity (PT50%); 4) non-sensitized and trained at 75% maximum speed _ moderate intensity (PT75%); 5) OA-sensitized and trained at 50% (OA+PT50%), 6) OA-sensitized and trained at 75% (OA+PT75%). There was no difference in muscle fiber cross-sectional area among groups and no difference in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise training; Muscle atrophy; Asthma; Gene expression; Mice.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400005

Transduction motif analysis of gastric cancer based on a human signaling network
Provedor de dados: 56
Autores: Liu,G.; Li,D.Z.; Jiang,C.S.; Wang,W.
To investigate signal regulation models of gastric cancer, databases and literature were used to construct the signaling network in humans. Topological characteristics of the network were analyzed by CytoScape. After marking gastric cancer-related genes extracted from the CancerResource, GeneRIF, and COSMIC databases, the FANMOD software was used for the mining of gastric cancer-related motifs in a network with three vertices. The significant motif difference method was adopted to identify significantly different motifs in the normal and cancer states. Finally, we conducted a series of analyses of the significantly different motifs, including gene ontology, function annotation of genes, and model classification. A human signaling network was constructed,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Significantly different motifs; Human signaling network; Gastric cancer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500369

Two research paths for probing the roles of oxygen in metabolic regulation
Provedor de dados: 56
Autores: Hochachka,P.W.
Tissues such as skeletal and cardiac muscles must sustain very large-scale changes in ATP turnover rate during equally large changes in work. In many skeletal muscles these changes can exceed 100-fold. Examination of a number of cell and whole-organism level systems identifies ATP concentration as a key parameter of the interior milieu that is nearly universally 'homeostatic'; it is common to observe no change in ATP concentration even while change in its turnover rate can increase or decrease by two orders of magnitude or more. A large number of other intermediates of cellular metabolism are also regulated within narrow concentration ranges, but none seemingly as precisely as is [ATP]. In fact, the only other metabolite in aerobic energy metabolism that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic regulation; Oxygen delivery; Oxygen regulation; Intracellular perfusion; Intracellular diffusion.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600001

Insect juvenile hormone: from "status quo" to high society
Provedor de dados: 56
Autores: Hartfelder,K.
Juvenile hormone (JH) exerts pleiotropic functions during insect life cycles. The regulation of JH biosynthesis by neuropeptides and biogenic amines, as well as the transport of JH by specific binding proteins is now well understood. In contrast, comprehending its mode of action on target organs is still hampered by the difficulties in isolating specific receptors. In concert with ecdysteroids, JH orchestrates molting and metamorphosis, and its modulatory function in molting processes has gained it the attribute "status quo" hormone. Whereas the metamorphic role of JH appears to have been widely conserved, its role in reproduction has been subject to many modifications. In many species, JH stimulates vitellogenin synthesis and uptake. In mosquitoes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Juvenile hormone; Corpora allata; Metamorphosis; Insect reproduction; Polymorphism; Honey bee.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200003

Potentiation of carbon tetrachloride hepatotoxicity by pentosan polysulfate in rats
Provedor de dados: 56
Autores: Zim,M.C.A.; Silveira,T.R.; Schwartsmann,G.; Cerski,T.; Motta,A.
Few data are available in the literature regarding the effect of pentosan polysulfate (PPS) on normal and fibrotic rat livers. In addition, the combination of PPS and carbon tetrachloride (CCl4) has not been studied so far. The objective of this study was to assess the effect of PPS on rat livers treated or not with CCl4 for the induction of liver fibrosis. The study consisted of four stages: 1) hepatic fibrosis induction with CCl4 (N = 36 rats); 2) evaluation of the effect of PPS on CCl4-induced hepatic fibrosis (N = 36 rats); 3) evaluation of the effect of higher doses of PPS in combination with CCl4 (N = 50 rats); 4) evaluation of the presence of an enzymatic inductor effect by PPS (N = 18 rats) using the sodium pentobarbital test which indirectly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cirrhosis; Fibrogenesis; Hepatic necrosis; Pentosan polysulfate; Carbon tetrachloride.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100012

Postangioplasty restenosis: a practical model in the porcine carotid artery
Provedor de dados: 56
Autores: Caramori,P.R.A.; Eggers,E.E.; Silva-Filho,A.P.F.; Uchoa,D.M.; Jung,F.; Zago,A.C.; Cerski,C.T.S.; Schwartsmann,G.; Zago,A.J.
Transluminal coronary angioplasty is a routine therapeutic intervention in coronary heart disease. Despite the high rate of primary success, restenosis continues to be its major limitation. Porcine models have been considered to be the most adequate experimental models for studying restenosis. One limitation of porcine models is the need for radiological guidance and the expenses involved. The objective of the present study was to adapt an experimental model of angioplasty in the porcine carotid artery that does not require radiological equipment. Eight animals were used to develop the technique of balloon injury to the common carotid artery by dissection without radiological guidance. This technique was then employed in six other animals. Under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arterial injury; Intimal hyperplasia; Restenosis; Percutaneous transluminal coronary angioplasty.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900007

Clinical and molecular analysis of human reproductive disorders in Brazilian patients
Provedor de dados: 56
Autores: Latronico,A.C.; Costa,E.M.F.; Domenice,S.; Correa,R.V.; Kohek,M.B.F.; Arnhold,I.J.P.; Mendonca,B.B.
Several genes that influence the development and function of the hypothalamic-pituitary-gonadal-axis (HPG) have been identified. These genes encode an array of transcription factors, matrix proteins, hormones, receptors, and enzymes that are expressed at multiple levels of the HPG. We report the experience of a single Endocrinology Unit in the identification and characterization of naturally occurring mutations in families affected by HPG disorders, including forms of precocious puberty, hypogonadism and abnormal sexual development due to impaired gonadotropin function. Eight distinct genes implicated in HPG function were studied: KAL, SF1, DAX1, GnRH, GnRHR, FSHß, FSHR, and LHR. Most mutations identified in our cohort are described for the first time in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypogonadism; Puberty; Gonadotropins; Genetic mutation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100019

MAFB mediates the therapeutic effect of sleeve gastrectomy for obese diabetes mellitus by activation of FXR expression
Provedor de dados: 56
Autores: Xu,Jian; Wang,Yong; Yin,Jiajun; Yin,Min; Wang,Mofei; Liu,Jingang.
Farnesoid X receptor (FXR) and related pathways are involved in the therapeutic effect of sleeve gastrectomy for overweight or obese patients with diabetes mellitus. This study aimed to investigate the mechanism of FXR expression regulation during the surgical treatment of obese diabetes mellitus by sleeve gastrectomy. Diabetic rats were established by combined streptozotocin and high-fat diet induction. Data collection included body weight, chemical indexes of glucose and lipid metabolism, liver function, and the expression levels of musculoaponeurotic fibrosarcoma oncogene family B (MAFB), FXR, and related genes induced by sleeve gastrectomy. Chang liver cells overexpressing MAFB gene were established to confirm the expression of related genes. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Obesity; Sleeve gastrectomy; MAFB; FXR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700612

Membrane proteins: functional and structural studies using reconstituted proteoliposomes and 2-D crystals
Provedor de dados: 56
Autores: Rigaud,J.-L.
Reconstitution of membrane proteins into lipid bilayers is a powerful tool to analyze functional as well as structural areas of membrane protein research. First, the proper incorporation of a purified membrane protein into closed lipid vesicles, to produce proteoliposomes, allows the investigation of transport and/or catalytic properties of any membrane protein without interference by other membrane components. Second, the incorporation of a large amount of membrane proteins into lipid bilayers to grow crystals confined to two dimensions has recently opened a new way to solve their structure at high resolution using electron crystallography. However, reconstitution of membrane proteins into functional proteoliposomes or 2-D crystallization has been an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Membrane proteins; Proteoliposomes; 2-D crystals; Electron crystallography; Reconstitution.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700001

H1 but not H2 histamine antagonist receptors mediate anxiety-related behaviors and emotional memory deficit in mice subjected to elevated plus-maze testing
Provedor de dados: 56
Autores: Serafim,K.R.; Kishi,M.S.; Canto-de-Souza,A.; Mattioli,R.
This study investigated the role of H1 and H2 receptors in anxiety and the retrieval of emotional memory using a Trial 1/Trial 2 (T1/T2) protocol in an elevated plus-maze (EPM). Tests were performed on 2 consecutive days, designated T1 and T2. Before T1, the mice received intraperitoneal injections of saline (SAL), 20 mg/kg zolantidine (ZOL, an H2receptor antagonist), or 8.0 or 16 mg/kg chlorpheniramine (CPA, an H1 receptor antagonist). After 40 min, they were subjected to the EPM test. In T2 (24 h later), each group was subdivided into two additional groups, and the animals from each group were re-injected with SAL or one of the drugs. In T1, the Student t-test showed no difference between the SAL and ZOL or 8 mg/kg CPA groups with respect to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorpheniramine; Zolantidine; Anxiety; Memory; Elevated plus-maze.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500440

The combination of atorvastatin and ethanol is not more hepatotoxic to rats than the administration of each drug alone
Provedor de dados: 56
Autores: Ito,D.T.; Molina,H.M.; Andriolo,A.; Borges,D.R.
Animal studies and premarketing clinical trials have revealed hepatotoxicity of statins, primarily minor elevations in serum alanine aminotransferase levels. The combined chronic use of medicines and eventual ethanol abuse are common and may present a synergistic action regarding liver injury. Our objective was to study the effect of the chronic use of atorvastatin associated with acute ethanol administration on the liver in a rat model. One group of rats was treated daily for 5 days a week for 2 months with 0.8 mg/kg atorvastatin by gavage. At the end of the treatment the livers were perfused with 72 mM ethanol for 60 min. Control groups (at least 4 animals in each group) consisted of a group of 2-month-old male Wistar EPM-1 rats exposed to 10% ethanol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atorvastatin; Liver injury; Hepatotoxic; Alcohol.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300009

High prevalence of the GSTM3*A/B polymorphism in sub-Sarahan African populations
Provedor de dados: 56
Autores: Teixeira,D.; Vargens,D.; Príncipe,A.; Oliveira,E.; Amorim,A.; Prata,M.J.; Suarez-Kurtz,G.
A 3-bp insertion/deletion polymorphism in intron 6 of GSTM3 (rs1799735, GSTM3*A/*B) affects the activity of the phase 2 xenobiotic metabolizing enzyme GSTM3 and has been associated with increased cancer risk. The GSTM3*B allele is rare or absent in Southeast Asians, occurs in 5-20% of Europeans but was detected in 80% of Bantu from South Africa. The wide genetic diversity among Africans led us to investigate whether the high frequency of GSTM3*B prevailed in other sub-Saharan African populations. In 168 healthy individuals from Angola, Mozambique and the São Tomé e Príncipe islands, the GSTM3*B allele was three times more frequent (0.74-0.78) than the GSTM3*A allele (0.22-0.26), with no significant differences in allele frequency across the three groups....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: African populations; GSTM3 polymorphisms; Multidimensional scaling analysis; Pharmacogenetics; Population diversity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700010

Cytomegalovirus infection in renal transplant recipients diagnosed by nested-PCR
Provedor de dados: 56
Autores: Aquino,V.H.; Figueiredo,L.T.M.
A prospective study of cytomegalovirus (CMV) infection was carried out on 34 renal transplant recipients managed at a General Hospital in Ribeirão Preto, SP, Brazil. Serologic tests showed that all patients were infected with CMV before renal transplantation. Two nested-PCR techniques with primers that recognize sequences of the glycoprotein B (gB) and H (gH) genes were used for CMV detection in blood and urine samples during the post-transplantation period. CMV was detected more frequently in blood samples than in urine samples (P&lt;0.001). Thirty-three patients had CMV detected at least once in blood and/or urine samples. Seven of these patients (21.2%) were diagnosed as having symptomatic CMV infection and showed a worse clinical outcome, with a higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytomegalovirus; Polymerase chain reaction; Kidney transplantation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100011

Heparan sulfate and cell division
Provedor de dados: 56
Autores: Porcionatto,M.A.; Nader,H.B.; Dietrich,C.P.
Heparan sulfate is a component of vertebrate and invertebrate tissues which appears during the cytodifferentiation stage of embryonic development. Its structure varies according to the tissue and species of origin and is modified during neoplastic transformation. Several lines of experimental evidence suggest that heparan sulfate plays a role in cellular recognition, cellular adhesion and growth control. Heparan sulfate can participate in the process of cell division in two distinct ways, either as a positive or negative modulator of cellular proliferation, or as a response to a mitogenic stimulus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heparan sulfate; Glycosaminoglycan; Proteoglycan; Cell division; Cell cycle; Growth factors.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500006

DNA topoisomerase inhibitors: biflavonoids from Ouratea species
Provedor de dados: 56
Autores: Grynberg,N.F.; Carvalho,M.G.; Velandia,J.R.; Oliveira,M.C.; Moreira,I.C.; Braz- Filho,R.; Echevarria,A.
Topoisomerase inhibitors are agents with anticancer activity. 7"-O-Methyl-agathisflavone (I) and amentoflavone (II) are biflavonoids and were isolated from the Brazilian plants Ouratea hexasperma and O. semiserrata, respectively. These biflavonoids and the acetyl derivative of II (IIa) are inhibitors of human DNA topoisomerases I at 200 µM, as demonstrated by the relaxation assay of supercoiled DNA, and only agathisflavone (I) at 200 µM also inhibited DNA topoisomerases II-alpha, as observed by decatenation and relaxation assays. The biflavonoids showed concentration-dependent growth inhibitory activities on Ehrlich carcinoma cells in 45-h culture, assayed by a tetrazolium method, with IC50 = 24 ± 1.4 µM for I, 26 ± 1.1 µM for II and 10 ± 0.7 µM for IIa....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Biflavonoids; Amentoflavone; 7"-O-Methyl-agathisflavone; DNA topoisomerases; Ehrlich carcinoma; Human K562 leukemia.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700009

Modulation of store-operated Ca2+ entry by cyclic-ADP-ribose
Provedor de dados: 56
Autores: Thompson,M.; White,T.; Chini,E.N.
Store-operated Ca2+ entry plays an important role in Ca2+ homeostasis in cells but the mechanisms of control of these channels are not completely understood. We describe an investigation of the role of the CD38-cyclic-ADP-ribose (cADPR)-ryanodine-channel (RyR) signaling pathway in store-operated Ca2+ entry in human smooth muscle. We observed that human myometrial cells have a functional store-operated Ca2+ entry mechanism. Furthermore, we observed the presence of transient receptor potential 1, 3, 4, 5, and 6 ion channels in human myometrial cells. Store-operated Ca2+ transient was inhibited by at least 50-70% by several inhibitors of the RyR, including ryanodine (10 µM), dantrolene (10 µM), and ruthenium red (10 µM). Furthermore, the cell permeable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclic-ADP-ribose; IP3; Endoplasmic reticulum; Ryanodine channel; Calcium entry; Human smooth muscle.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600006

Atropine and ODQ antagonize tetanic fade induced by L-arginine in cats
Provedor de dados: 56
Autores: Cruciol-Souza,J.M.; Alves-Do-Prado,W.
Although it has been demonstrated that nitric oxide (NO) released from sodium nitrite induces tetanic fade in the cat neuromuscular preparations, the effect of L-arginine on tetanic fade and its origin induced by NO have not been studied in these preparations. Furthermore, atropine reduces tetanic fade induced by several cholinergic and anticholinergic drugs in these preparations, whose mechanism is suggested to be mediated by the interaction of acetylcholine with inhibitory presynaptic muscarinic receptors. The present study was conducted in cats to determine the effects of L-arginine alone or after pretreatment with atropine or 1H-[1,2,4]oxadiazole [4,3-a]quinoxalin-1-one (ODQ) on neuromuscular preparations indirectly stimulated at high frequency. Drugs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atropine; Tetanic fade; Skeletal muscle; Nitric oxide; Muscarinic receptors.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000015

Prokaryotic expression of a novel mouse pro-apoptosis protein PNAS-4 and application of its polyclonal antibodies
Provedor de dados: 56
Autores: Zhang,P.; Wang,C.T.; Yan,F.; Gou,L.; Tong,A.P.; Cai,F.; Li,Q.; Deng,H.X.; Wei,Y.Q.
Mouse PNAS-4 (mPNAS-4) has 96% identity with human PNAS-4 (hPNAS-4) in primary sequence and has been reported to be involved in the apoptotic response to DNA damage. However, there have been no studies reported of the biological functions of mPNAS-4. In studies conducted by our group (unpublished data), it was interesting to note that overexpression of mPNAS-4 promoted apoptotic death in Lewis lung carcinoma cells (LL2) and colon carcinoma cells (CT26) of mice both in vitro and in vivo. In our studies, mPNAS-4 was cloned into the pGEX-6P-1 vector with GST tag at N-terminal in Escherichia coli strain BL21(DE3). The soluble and insoluble expression of recombinant protein mPNAS-4 (rmPNAS-4) was temperature-dependent. The majority of rmPNAS-4 was insoluble at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-mPNAS-4; Polyclonal antibody; Apoptosis-related protein family; One-step affinity purification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600012

Angiotensin-(1-7) relieved renal injury induced by chronic intermittent hypoxia in rats by reducing inflammation, oxidative stress and fibrosis
Provedor de dados: 56
Autores: Lu,W.; Kang,J.; Hu,K.; Tang,S.; Zhou,X.; Yu,S.; Xu,L.
We aimed to study the renal injury and hypertension induced by chronic intermittent hypoxia (CIH) and the protective effects mediated by angiotensin 1-7 [Ang(1-7)]. We randomly assigned 32 male Sprague-Dawley rats (body weight 180-200 g) to normoxia control, CIH, Ang(1-7)-treated normoxia, and Ang(1-7)-treated CIH groups. Systolic blood pressure (SBP) was monitored at the start and end of each week. Renal sympathetic nerve activity (RSNA) was recorded. CTGF and TGF-β were detected by immunohistochemistry and western blotting. Tissue parameters of oxidative stress were also determined. In addition, renal levels of interleukin-6, tumor necrosis factor-α, nitrotyrosine, and hypoxia-inducible factor-1α were determined by immunohistochemistry, immunoblotting,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ang(1-7); Renal injury; Obstructive sleep apnea; Inflammation; Oxidative stress; Fibrosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100604

Pharmacological study of anti-allergic activity of Syzygium cumini (L.) Skeels
Provedor de dados: 56
Autores: Brito,F.A.; Lima,L.A.; Ramos,M.F.S.; Nakamura,M.J.; Cavalher-Machado,S.C.; Siani,A.C.; Henriques,M.G.M.O.; Sampaio,A.L.F.
Myrtaceae is a plant family widely used in folk medicine and Syzygium and Eugenia are among the most important genera. We investigated the anti-allergic properties of an aqueous leaf extract of Syzygium cumini (L.) Skeels (SC). HPLC analysis revealed that hydrolyzable tannins and flavonoids are the major components of the extract. Oral administration of SC (25-100 mg/kg) in Swiss mice (20-25 g; N = 7/group) inhibited paw edema induced by compound 48/80 (50% inhibition, 100 mg/kg; P &lt;= 0.05) and, to a lesser extent, the allergic paw edema (23% inhibition, 100 mg/kg; P &lt;= 0.05). SC treatment also inhibited the edema induced by histamine (58% inhibition; P &lt;= 0.05) and 5-HT (52% inhibition; P &lt;= 0.05) but had no effect on platelet-aggregating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myrtaceae; Syzygium cumini; Allergy; Paw edema; Pleurisy; Cytokine.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100014

Dipyridamole increases the cytotoxicity of cisplatin in human larynx cancer cells in vitro
Provedor de dados: 56
Autores: Rodrigues,M.; Barbosa Junior,F.; Perussi,J.R.
This paper describes the effect of dipyridamole (DIP) on the cytotoxicity of cisplatin in HEp-2 human larynx cancer cells in vitro and the nature of the interaction between cisplatin and dipyridamole. Cytotoxic assays were performed to obtain the IC50 for cisplatin. The cells were treated with 0, 20, 40, 80, 120 or 200 µM cisplatin, with or without a single concentration of DIP and incubated for 60 min at 37ºC and 5% CO2 for 3 days and then counted with a hemocytometer. The accumulation of cisplatin in the cells was measured by atomic absorption and fluorescence was used to determine the membrane binding constant of DIP. In the presence of 10, 20 and 30 µM DIP, the IC50 of cisplatin was reduced by 25, 60 and 82% in HEp-2 cells. Combination index analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cisplatin; Dipyridamole; Synergism; Tumor cells; Cytotoxicity; IC50.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400017

Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells
Provedor de dados: 56
Autores: Silva,N.P.; Christofolini,D.M.; Mortara,R.A.; Andrade,L.E.C.
Cajal bodies (CB) are ubiquitous nuclear structures involved in the biogenesis of small nuclear ribonucleoproteins and show narrow association with the nucleolus. To identify possible relationships between CB and the nucleolus, the localization of coilin, a marker of CB, and of a set of nucleolar proteins was investigated in cultured PtK2 cells undergoing micronucleation. Nocodazol-induced micronucleated cells were examined by double indirect immunofluorescence with antibodies against coilin, fibrillarin, NOR-90/hUBF, RNA polymerase I, PM/Scl, and To/Th. Cells were imaged on a BioRad 1024-UV confocal system attached to a Zeiss Axiovert 100 microscope. Since PtK2 cells possess only one nucleolus organizer region, micronucleated cells presented only one or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cajal body; Coiled body; Micronuclei; Cell nucleolus; Nucleolus organizer region; RNA polymerase I; Nucleolar proteins.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700008

Computed tomography findings of hepatic veno-occlusive disease caused by Sedum aizoon with histopathological correlation
Provedor de dados: 56
Autores: Shao,H.; Chen,H. Z.; Zhu,J. S.; Ruan,B.; Zhang,Z. Q.; Lin,X.; Gan,M. F.
This study investigated the value of computed tomography (CT) in the diagnosis and treatment of hepatic veno-occlusive disease (HVOD) caused by Sedum aizoon (SA). The clinical manifestations, treatment results, imaging findings, and histological findings of the liver were analyzed in 39 patients with HVOD caused by SA. Hepatomegaly, liver dysfunction, abdominal effusion, and geographic density changes on liver CT scans were found in all 39 patients. The pathological findings of histological liver examination included swelling and point-like necrosis of liver cells, significant expansion and congestion of the sinuses, endothelial swelling, and wall thickening with incomplete lumen occlusion of small liver vessels. CT geographic density changes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic veno-occlusive disease; Sedum aizoon; Drug-induced liver disease; Computed tomography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201145

Design and applications of modified oligonucleotides
Provedor de dados: 56
Autores: Gallo,M.; Montserrat,J.M.; Iribarren,A.M.
Oligonucleotides have a wide range of applications in fields such as biotechnology, molecular biology, diagnosis and therapy. However, the spectrum of uses can be broadened by introducing chemical modifications into their structures. The most prolific field in the search for new oligonucleotide analogs is the antisense strategy, where chemical modifications confer appropriate characteristics such as hybridization, resistance to nucleases, cellular uptake, selectivity and, basically, good pharmacokinetic and pharmacodynamic properties. Combinatorial technology is another research area where oligonucleotides and their analogs are extensively employed. Aptamers, new catalytic ribozymes and deoxyribozymes are RNA or DNA molecules individualized from a randomly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Modified nucleotides; Antisense; Ribozymes; Oligonucleotide libraries; Aptamers; SELEX method.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200001

Protective effect and mechanism of Lactobacillus on cerebral ischemia reperfusion injury in rats
Provedor de dados: 56
Autores: Wanchao,Shi; Chen,Ma; Zhiguo,Su; Futang,Xie; Mengmeng,Shi.
The present study was designed to investigate the protective effects and mechanism of inactivated lactobacillus (ILA) on cerebral ischemia reperfusion injury (CIRI) in rats. In this experiment, 30 male Sprague Dawley rats were randomly divided into control group, IRI groups, and ILA group. A middle cerebral artery occlusion and reperfusion model was prepared. The rats were killed after 24 hours of recovery of blood flow of cerebral ischemia resulting from 60-min occlusion. The cerebral infarction volume and neurological scores were assayed by staining and behavioral observation. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were assayed by biochemical kits. Cell apoptosis was assayed by Tunnel and the Toll-like receptor (TLR)-4, IkB, and A20...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebral ischemia reperfusion injury; Inactivated Lactobacillus; Apoptosis; NF-kappa B; TLR-4.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700607

Transcriptional regulation of bidirectional gene pairs by 17-β-estradiol in MCF-7 breast cancer cells
Provedor de dados: 56
Autores: Garcia,S.A.B.; Nagai,M.A.
Using cDNA microarray analysis, we previously identified a set of differentially expressed genes in primary breast tumors based on the status of estrogen and progesterone receptors. In the present study, we performed an integrated computer-assisted and manual search of potential estrogen response element (ERE) binding sites in the promoter region of these genes to characterize their potential to be regulated by estrogen receptors (ER). Publicly available databases were used to annotate the position of these genes in the genome and to extract a 5’flanking region 2 kb upstream to 2 kb downstream of the transcription start site for transcription binding site analysis. The search for EREs and other binding sites was performed using several publicly available...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Estrogen receptor; Gene expression; Bidirectional promoters.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200005

The control of deliberate waiting strategies in a stop-signal task
Provedor de dados: 56
Autores: Sylwan,R.P.
To inhibit an ongoing flow of thoughts or actions has been largely considered to be a crucial executive function, and the stop-signal paradigm makes inhibitory control measurable. Stop-signal tasks usually combine two concurrent tasks, i.e., manual responses to a primary task (go-task) are occasionally countermanded by a stimulus which signals participants to inhibit their response in that trial (stop-task). Participants are always instructed not to wait for the stop-signal, since waiting strategies cause the response times to be unstable, invalidating the data. The aim of the present study was to experimentally control the strategies of waiting deliberately for the stop-signal in a stop-task by means of an algorithm that measured the variation in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attention; Stop-task; Reaction time; Vision; Executive functions; Laterality.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600011

Efficacy of cognitive behavioral therapy in reducing psychiatric symptoms in patients with implantable cardioverter defibrillator: an integrative review
Provedor de dados: 56
Autores: Maia,A.C.C.O.; Braga,A.A.; Soares-Filho,G.; Pereira,V.; Nardi,A.E.; Silva,A.C.
This article is a systematic review of the available literature on the benefits that cognitive behavioral therapy (CBT) offers patients with implanted cardioverter defibrillators (ICDs) and confirms its effectiveness. After receiving the device, some patients fear that it will malfunction, or they remain in a constant state of tension due to sudden electrical discharges and develop symptoms of anxiety and depression. A search with the key words “anxiety”, “depression”, “implantable cardioverter”, “cognitive behavioral therapy” and “psychotherapy” was carried out. The search was conducted in early January 2013. Sources for the search were ISI Web of Knowledge, PubMed, and PsycINFO. A total of 224 articles were retrieved: 155 from PubMed, 69 from ISI Web of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anxiety; Depression; Cognitive behavioral therapy; Psychotherapy; Cardiology; Implantable cardioverter defibrillators.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400265

The role of adrenergic receptor polymorphisms in heart failure
Provedor de dados: 56
Autores: Biolo,A.; Rosa,A.S.; Mazzotti,N.G.; Martins,S.; Belló-Klein,A.; Rohde,L.E.; Clausell,N.
The main function of the cardiac adrenergic system is to regulate cardiac work both in physiologic and pathologic states. A better understanding of this system has permitted the elucidation of its role in the development and progression of heart failure. Regardless of the initial insult, depressed cardiac output results in sympathetic activation. Adrenergic receptors provide a limiting step to this activation and their sustained recruitment in chronic heart failure has proven to be deleterious to the failing heart. This concept has been confirmed by examining the effect of ß-blockers on the progression of heart failure. Studies of adrenergic receptor polymorphisms have recently focused on their impact on the adrenergic system regarding its adaptive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Adrenergic receptor; Polymorphisms.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001000003

Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees
Provedor de dados: 56
Autores: Torres,A.R.; Sandjo,L.P.; Friedemann,M.T.; Tomazzoli,M.M.; Maraschin,M.; Mello,C.F.; Santos,A.R.S.
In this study, we investigated the chemical composition, and antioxidant and antibacterial properties of ethanolic extracts of propolis (EEP) from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula. Chemical composition of EEP was determined by colorimetry and chromatographic (HPLC-DAD and UPLC-Q/TOF-MS/MS) analysis. Antimicrobial activity of EEP was evaluated against gram-positive (S. aureus, methicillin-resistant S. aureus, E. faecalis) and gram-negative (E. coli and K. pneumoniae) bacteria by the minimal inhibitory concentration (MIC) test using the microdilution method. Furthermore, the growth curve and integrity of cell membrane of S. aureus and E. coli were investigated using standard microbiological methods. HPLC-DAD analysis showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stingless bees; Propolis; Antimicrobial activity; S. aureus; M. quadrifasciata quadrifasciata; T. angustula.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600617

Sulfated fucan as support for antibiotic immobilization
Provedor de dados: 56
Autores: Araújo,P.M.; Oliveira,G.B.; Córdula,C.R.; Leite,E.L.; Carvalho Jr.,L.B.; Silva,M.P.C.
Xylofucoglucuronan from Spatoglossum schröederi algae was tested as a support for antibiotic immobilization. The polysaccharide (20 mg in 6 ml) was first activated using carbodiimide, 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide methiodide (20 mg in 2 ml), under stirring for 1 h at 25ºC and pH from 4.5 to 5.0. After adjusting the pH to 8.0, either gentamicin or amikacin (62.5 mg in 1.25 ml) was then immobilized on this chemically modified polysaccharide with shaking for 24 h in a cold room. Infrared spectra of the activated carbodiimide xylofucoglucuronan showed two bands to carbonyl (C = O at 1647.9 and 1700.7 cm-1) and to amide (C&lt;FONT FACE=Symbol&gt;Ý&lt;/FONT&gt;-NH2) groups (1662.8 and 1714.0 cm-1). Microbial characterization of the derivatives...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sulfated fucan; Immobilization; Antibiotics; Gentamicin; Amikacin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300002

Classification of different degrees of adiposity in sedentary rats
Provedor de dados: 56
Autores: Leopoldo,A.S.; Lima-Leopoldo,A.P.; Nascimento,A.F.; Luvizotto,R.A.M.; Sugizaki,M.M.; Campos,D.H.S.; da Silva,D.C.T.; Padovani,C.R.; Cicogna,A.C.
In experimental studies, several parameters, such as body weight, body mass index, adiposity index, and dual-energy X-ray absorptiometry, have commonly been used to demonstrate increased adiposity and investigate the mechanisms underlying obesity and sedentary lifestyles. However, these investigations have not classified the degree of adiposity nor defined adiposity categories for rats, such as normal, overweight, and obese. The aim of the study was to characterize the degree of adiposity in rats fed a high-fat diet using cluster analysis and to create adiposity intervals in an experimental model of obesity. Thirty-day-old male Wistar rats were fed a normal (n=41) or a high-fat (n=43) diet for 15 weeks. Obesity was defined based on the adiposity index; and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Adiposity index; Rats; Cluster analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400601

Systematic head and neck physical examination as a predictor of obstructive sleep apnea in class III obese patients
Provedor de dados: 56
Autores: Martinho,F.L.; Tangerina,R.P.; Moura,S.M.G.T.; Gregório,L.C.; Tufik,S.; Bittencourt,L.R.A.
Our aim was to determine if anatomical abnormalities of the upper airway (UA) and facial skeleton of class III severely obese patients are related to the presence and severity of obstructive sleep apnea syndrome (OSAS). Forty-five patients (69% females, mean age 46.5 ± 10.8 years) with a body mass index (BMI) over 40 kg/m² underwent UA and facial skeletal examinations as well as polysomnography. Mean BMI was 49 ± 7 kg/m² and mean neck circumference was 43.4 ± 5.1 cm. Polysomnographic findings showed that 22% had a normal apnea-hypopnea index (AHI) and 78% had an AHI over 5. The presence of OSAS was associated with younger age (P = 0.02), larger neck circumference (P = 0.004), presence of a voluminous lateral wall (P = 0.0002), posteriorized soft palate (P...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Obstructive sleep apnea syndrome; Snore; Physical examination; Predictors.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200008

A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide
Provedor de dados: 56
Autores: Ramos,C.R.R.; Abreu,P.A.E.; Nascimento,A.L.T.O.; Ho,P.L.
We report here the construction of a vector derived from pET3-His and pRSET plasmids for the expression and purification of recombinant proteins in Escherichia coli based on T7 phage RNA polymerase. The resulting pAE plasmid combined the advantages of both vectors: small size (pRSET), expression of a short 6XHis tag at N-terminus (pET3-His) and a high copy number of plasmid (pRSET). The small size of the vector (2.8 kb) and the high copy number/cell (200-250 copies) facilitate the subcloning and sequencing procedures when compared to the pET system (pET3-His, 4.6 kb and 40-50 copies) and also result in high level expression of recombinant proteins (20 mg purified protein/liter of culture). In addition, the vector pAE enables the expression of a fusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Expression vector; Immobilized metal affinity chromatography; Protein purification.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800001

Molecular epidemiology of type 1 and 2 dengue viruses in Brazil from 1988 to 2001
Provedor de dados: 56
Autores: Pires Neto,R.J.; Lima,D.M.; de Paula,S.O.; Lima,C.M.; Rocco,I.M.; Fonseca,B.A.L.
Dengue is a mosquito-borne viral infection that in recent decades has become a major international public health concern. Epidemic dengue fever reemerged in Brazil in 1981. Since 1990 more than one dengue virus serotype has been circulating in this tropical country and increasing rates of dengue hemorrhagic fever and dengue shock syndrome have been detected every year. Some evidence supports the association between the introduction of a new serotype and/or genotype in a region and the appearance of dengue hemorrhagic fever. In order to study the evolutionary relationships and possible detection of the introduction of new dengue virus genotypes in Brazil in the last years, we analyzed partial nucleotide sequences of 52 Brazilian samples of both dengue type...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dengue virus; Nucleotide sequence; E/NS1 region; Molecular epidemiology; Dengue fever; Dengue hemorrhagic fever.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600005

Overexpression of kermit/dGIPC is associated with lethality in Drosophila melanogaster
Provedor de dados: 56
Autores: Pereira,G.B.; Valente,V.; de Queiroz,M.S.; Vianna,M.C.B.; Paçó-Larson,M.L.
Insertional mutagenesis is an important tool for functional genomics in Drosophila melanogaster. The insertion site in the KG00562 mutant fly line has been mapped to the CG8709 (herein named DmLpin) locus and to the 3’ of kermit (also called dGIPC). This mutant line presents a high lethality rate resulting from a gain of function. To obtain some insight into the biological role of the mutated locus, we have characterized the mutation and its relation to the high mortality of the KG00562 fly line. In this mutant, we did not detect one of the DmLpin transcripts, namely DmLpinK, but we did detect an unusual 2.3-kb mRNA (LpinK-w). Further investigation revealed that the LpinK-w transcript results from an aberrant splicing between the untranslated first exon of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insertional mutagenesis; Aberrant splicing; DmLpin; Kermit/dGIPC.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400003

The rs878081 polymorphism of AIRE gene increases the risk of rheumatoid arthritis in a Chinese Han population: a case-control study
Provedor de dados: 56
Autores: Yang,Haoyu; Li,Jin; Jiang,Lifeng; Jiang,Xijia; Zhou,Xindie; Xu,Nanwei.
The autoimmune regulator (AIRE), a transcriptional regulator expressed in medullary thymic epithelial cells, plays an important role in thymocyte education and negative selection. Several citations studying the association between the rs878081 exon polymorphism of the AIRE gene and the risk of rheumatoid arthritis (RA) in different populations have yielded conflicting findings. Thus, this case-control study involving 300 RA cases and 300 controls was aimed to identify whether such association existed in a Chinese Han population from East China. The rs878081 polymorphism of the AIRE gene was genotyped. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated using the chi-squared test, genetic model analysis, and stratification analysis. Genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AIRE; Single-nucleotide polymorphism; Rheumatoid arthritis; Case-control study.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200612

The impact of short daily hemodialysis on anemia and the quality of life in Chinese patients
Provedor de dados: 56
Autores: Jiang,J.L.; Ren,W.; Song,J.; Sun,Q.L.; Xiao,X.Y.; Diao,X.Z.; Huang,Y.H.; Lan,L.; Wang,P.; Hu,Z.
Anemia is a frequent complication in hemodialysis patients. Compared to conventional hemodialysis (CHD), short daily hemodialysis (sDHD) has been reported to be effective in many countries except China. The aim of the present study was to determine whether sDHD could improve anemia and quality of life (QOL) for Chinese outpatients with end-stage renal disease. Twenty-seven patients (16 males/11 females) were converted from CHD to sDHD. All laboratory values were measured before conversion (baseline), at 3 months after conversion (sDHD1), and at 6 months after conversion (sDHD2). The patient's QOL was evaluated at baseline and 6 months after conversion using the Medical Outcomes Study 36-Item Short Form Health Survey (SF-36). Hemoglobin concentration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Short daily hemodialysis; Anemia; Erythropoietin; Quality of life; End-stage renal disease.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700629

How frequently should a patient taking amiodarone be screened for thyroid dysfunction?
Provedor de dados: 56
Autores: Pazin-Filho,A.; de Jesus,A.M.X.; Magalhães,P.K.R.; Melato,L.H.; Campos,D.; Maciel,B.C.; Maciel,L.M.Z.
Amiodarone-induced thyroid dysfunction (AITD) is a common complication of amiodarone therapy and its prevalence varies according to iodine intake, subclinical thyroid disorders and the definition of AITD. There is no consensus about the frequency of screening for this condition. We evaluated 121 patients on chronic regular intake of amiodarone (mean intake = 248.5 ± 89 mg; duration of treatment = 5.3 ± 3.9 years, range = 0.57-17 years) and with stable baseline cardiac condition. Those with no AITD were followed up for a median period of 3.2 years (range: 0.6-6.7) and the incidence rate of AITD, defined by clinical and laboratorial findings as proposed by international guidelines, was obtained (62.8 per 1000 patients/year). We applied the Cox proportional...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amiodarone; Chagas’ disease; Thyroid dysfunction; Antiarrhythmic drug.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800009

Ureases display biological effects independent of enzymatic activity: Is there a connection to diseases caused by urease-producing bacteria?
Provedor de dados: 56
Autores: Olivera-Severo,D.; Wassermann,G.E.; Carlini,C.R.
Ureases are enzymes from plants, fungi and bacteria that catalyze the hydrolysis of urea to form ammonia and carbon dioxide. While fungal and plant ureases are homo-oligomers of 90-kDa subunits, bacterial ureases are multimers of two or three subunit complexes. We showed that some isoforms of jack bean urease, canatoxin and the classical urease, bind to glycoconjugates and induce platelet aggregation. Canatoxin also promotes release of histamine from mast cells, insulin from pancreatic cells and neurotransmitters from brain synaptosomes. In vivo it induces rat paw edema and neutrophil chemotaxis. These effects are independent of ureolytic activity and require activation of eicosanoid metabolism and calcium channels. Helicobacter pylori, a Gram-negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urease; Canatoxin; Helicobacter pylori; Inflammation; Neutrophils; Eicosanoids.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700002

Effect of high-fat diets on body composition, lipid metabolism and insulin sensitivity, and the role of exercise on these parameters
Provedor de dados: 56
Autores: Coelho,D.F.; Pereira-Lancha,L.O.; Chaves,D.S.; Diwan,D.; Ferraz,R.; Campos-Ferraz,P.L.; Poortmans,J.R.; Lancha Junior,A.H.
Dietary fat composition can interfere in the development of obesity due to the specific roles of some fatty acids that have different metabolic activities, which can alter both fat oxidation and deposition rates, resulting in changes in body weight and/or composition. High-fat diets in general are associated with hyperphagia, but the type of dietary fat seems to be more important since saturated fats are linked to a positive fat balance and omental adipose tissue accumulation when compared to other types of fat, while polyunsaturated fats, omega-3 and omega-6, seem to increase energy expenditure and decrease energy intake by specific mechanisms involving hormone-sensitive lipase, activation of peroxisome proliferator-activated receptor α (PPARα) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fat; Obesity; Insulin; PUFA; Diet; Exercise.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000001

Chronic intermittent exposure to ayahuasca during aging does not affect memory in mice
Provedor de dados: 56
Autores: Correa-Netto,N.F.; Coelho,L.S.; Galfano,G.S.; Nishide,F.; Tamura,F.; Shimizu,M.K.; Santos Junior,J.G.; Linardi,A.
The Quechua term ayahuasca refers to a beverage obtained from decoctions of the liana Banisteriopsis caapi with leaves of Psychotria viridis. The ritualistic use of ayahuasca is becoming a global phenomenon, with some individuals using this beverage throughout life, including in old age. Cognitive impairment is a common manifestation during aging. There are conflicting reports on the ability of some ayahuasca compounds to exert neuroprotective or neurotoxic effects that could improve or impair learning and memory. Animal models provide a relevant and accessible means of investigating the behavioral effects of ayahuasca without the environmental conditions associated with the ritualistic use of the beverage. In this study, we investigated the influence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anxiety Banisteriopsis Carbolines N; N-dimethyltryptamine Learning.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700604

Meta-analysis of the efficacy of proton pump inhibitors for the symptoms of laryngopharyngeal reflux
Provedor de dados: 56
Autores: Liu,C.; Wang,H.; Liu,K.
The objective of this study was to perform a systematic review and meta-analysis to assess the effectiveness of proton pump inhibitors (PPI) for reflux disease in adult patients with laryngopharyngeal symptoms. A comprehensive search of Cochrane Library, EMBASE, Ovid EBM Reviews, and PubMed was performed for English-language literature about laryngopharyngeal reflux (LPR), in September 2014. The papers were filtered using pre-defined inclusion and exclusion criteria. Eight papers were identified and included in this meta-analysis. The sample comprised a pooled total of 370 patients, of which 210 and 160 patients took PPIs and placebo, respectively. The difference between PPIs and placebo groups in overall improvement of symptoms in adult patients with LPR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastroesophageal reflux disease; Adult; Proton pump inhibitors; Meta-analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700704

131I-induced changes in rat thyroid gland function
Provedor de dados: 56
Autores: Torlak,V.; Zemunik,T.; Modun,D.; Capkun,V.; Pesutic-Pisac,V.; Markotic,A.; Pavela-Vrancic,M.; Stanicic,A.
Therapeutic doses of 131I administered to thyrotoxic patients may cause thyroid failure. The present study used a rat model to determine thyroid function after the administration of different doses of 131I (64-277 µCi). Thirty male Fisher rats in the experimental group and 30 in the control group (untreated) were followed for 6 months. The animals were 4 months old at the beginning of the experiment and were sacrificed at an age of 9 months. Hormone concentration was determined before 131I administration (4-month-old animals) and three times following 131I administration, when the animals were 7, 8, and 9 months old. The thyroid glands were removed and weighed, their volume was determined and histopathological examination was performed at the end of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid gland; 131I; Hormones; Histopathology; T3; T4; TSH.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800009

Arginine induces GH gene expression by activating NOS/NO signaling in rat isolated hemi-pituitaries
Provedor de dados: 56
Autores: Olinto,S.C.F.; Adrião,M.G.; Castro-Barbosa,T.; Goulart-Silva,F.; Nunes,M.T.
The amino acid arginine (Arg) is a recognized secretagogue of growth hormone (GH), and has been shown to induce GH gene expression. Arg is the natural precursor of nitric oxide (NO), which is known to mediate many of the effects of Arg, such as GH secretion. Arg was also shown to increase calcium influx in pituitary cells, which might contribute to its effects on GH secretion. Although the mechanisms involved in the effects of Arg on GH secretion are well established, little is known about them regarding the control of GH gene expression. We investigated whether the NO pathway and/or calcium are involved in the effects of Arg on GH gene expression in rat isolated pituitaries. To this end, pituitaries from approximately 170 male Wistar rats (~250 g) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arginine; GH mRNA expression; Nitric oxide; Calcium (Ca2+); Cyclic guanosine monophosphate.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100011

Immunological and biochemical parameters of patients with metabolic syndrome and the participation of oxidative and nitroactive stress
Provedor de dados: 56
Autores: Simão,A.N.C.; Lozovoy,M.A.B.; Simão,T.N.C.; Venturini,D.; Barbosa,D.S.; Dichi,J.B.; Matsuo,T.; Cecchini,R.; Dichi,I.
Metabolic syndrome (MS) is a multifactorial disease involving inflammatory activity and endothelial dysfunction. The aim of the present study was to evaluate the relationship between the changes in lipoperoxidation, in immunological and biochemical parameters and nitric oxide metabolite (NOx) levels in MS patients. Fifty patients with MS (4 males/46 females) and 50 controls (3 males/47 females) were studied. Compared to control (Mann-Whitney test), MS patients presented higher serum levels (P &lt; 0.05) of fibrinogen: 314 (185-489) vs 262 (188-314) mg/dL, C-reactive protein (CRP): 7.80 (1.10-46.50) vs 0.70 (0.16-5.20) mg/dL, interleukin-6: 3.96 (3.04-28.18) vs 3.33 (2.55-9.63) pg/mL, uric acid: 5.45 (3.15-9.65) vs 3.81 (2.70-5.90) mg/dL, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic syndrome; Uric acid; Inflammation; Adiponectin; Nitric oxide; Lipoperoxidation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700015

Etofibrate but not controlled-release niacin decreases LDL cholesterol and lipoprotein (a) in type IIb dyslipidemic subjects
Provedor de dados: 56
Autores: Sposito,A.C.; Mansur,A.P.; Maranhão,R.C.; Rodrigues-Sobrinho,C.R.M.; Coelho,O.R.; Ramires,J.A.F.
Etofibrate is a hybrid drug which combines niacin with clofibrate. After contact with plasma hydrolases, both constituents are gradually released in a controlled-release manner. In this study, we compared the effects of etofibrate and controlled-release niacin on lipid profile and plasma lipoprotein (a) (Lp(a)) levels of patients with triglyceride levels of 200 to 400 mg/dl, total cholesterol above 240 mg/dl and Lp(a) above 40 mg/dl. These patients were randomly assigned to a double-blind 16-week treatment period with etofibrate (500 mg twice daily, N = 14) or niacin (500 mg twice daily, N = 11). In both treatment groups total cholesterol, VLDL cholesterol and triglycerides were equally reduced and high-density lipoprotein cholesterol was increased....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Etofibrate; Niacin; Lipoprotein (a); Triglycerides; Cholesterol.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200004

Effect of immobilization stress on gene expression of catecholamine biosynthetic enzymes in heart auricles of socially isolated rats
Provedor de dados: 56
Autores: Gavrilovic,L.; Spasojevic,N.; Zivkovic,M.; Dronjak,S.
Chronic stress is associated with the development of cardiovascular diseases. The sympathoneural system plays an important role in the regulation of cardiac function both in health and disease. In the present study, the changes in gene expression of the catecholamine biosynthetic enzymes tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) and protein levels in the right and left heart auricles of naive control and long-term (12 weeks) socially isolated rats were investigated by Taqman RT-PCR and Western blot analysis. The response of these animals to additional immobilization stress (2 h) was also examined. Long-term social isolation produced a decrease in TH mRNA level in left auricles (about 70%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart auricles; Tyrosine hydroxylase; Phenylethanolamine N-methyltransferase gene expression; Dopamine-β-hydroxylase.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200011

Nitrogen single-breath washout test for evaluating exercise tolerance and quality of life in patients with chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Deus,C.A.L.; Vigário,P.S.; Guimarães,F.S.; Lopes,A.J.
Pulmonary function tests (PFTs) traditionally used in clinical practice do not accurately predict exercise intolerance in patients with chronic obstructive pulmonary disease (COPD). The aim of this study was to assess whether the nitrogen single-breath washout (N2SBW) test explains exercise intolerance and poor quality of life in stable COPD patients. This cross-sectional study included 31 patients with COPD subjected to PFTs (including the N2SBW test) and a cardiopulmonary exercise test (CPET). Patients were also evaluated using the following questionnaires: the COPD assessment test (CAT), the 36-Item Short Form Health Survey (SF36) and St. George's Respiratory Questionnaire (SGRQ). Peak oxygen uptake (peak VO2) was negatively correlated with the phase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic obstructive pulmonary disease; Ventilatory efficiency; Pulmonary function tests; Cardiopulmonary exercise testing; Quality of life.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400604

Responsiveness of glycogen breakdown to cyclic AMP in perfused liver from rats with insulin-induced hypoglycemia
Provedor de dados: 56
Autores: Vardanega-Peicher,M.; Curi,R.; Pagliarini e Silva,S.; Nascimento,K.F.; Bazotte,R.B.
The responsiveness of glycogen breakdown to cAMP was investigated in isolated perfused liver from male Wistar fed rats (200-220 g) with insulin-induced hypoglycemia. The activation of glycogenolysis by 3 µM cAMP was decreased (P&lt;0.05) in livers from rats with hypoglycemia induced by the administration of insulin or during the direct infusion of insulin into the isolated liver. The direct effect of insulin on glycogen catabolism promoted by 3 µM cAMP occurred as early as 3 min after starting insulin infusion. In contrast, the cAMP agonists resistant to phosphodiesterases, 8Br-cAMP and 6MB-cAMP, used at the same concentration as cAMP, i.e., 3 µM, did not modify the effect of insulin. The data suggest that the decreased hepatic responsiveness of glycogen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin-induced hypoglycemia; Cyclic AMP; Glycogen breakdown; Liver metabolism.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100007

Leptin receptor (Ob-R) mRNA expression and serum leptin concentration in patients with colorectal and metastatic colorectal cancer
Provedor de dados: 56
Autores: Erkasap,N.; Ozkurt,M.; Erkasap,S.; Yasar,F.; Uzuner,K.; Ihtiyar,E.; Uslu,S.; Kara,M.; Bolluk,O.
The objective of the present study was to investigate the effect of leptin on the progression of colorectal carcinoma to metastatic disease by analyzing the serum leptin concentration and Ob-R gene expression in colon cancer tissues. Tissue samples were obtained from 31 patients who underwent surgical resection for colon (18 cases) and metastatic colon (13 cases) cancer. Serum leptin concentration was determined by an enzyme-linked immunosorbent assay (ELISA) and Ob-R mRNA expression by real-time polymerase chain reaction (RT-PCR) for both groups. ELISA data were analyzed by the Student t-test and RT-PCR data were analyzed by the Mann-Whitney U-test. RT-PCR results demonstrated that mRNA expression of Ob-R in human metastatic colorectal cancer was higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptin; Colorectal cancer; Metastatic colorectal cancer; Leptin receptor (Ob-R); Real-time PCR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300306

Cognitive performance of young and elderly subjects on the free word recall memory test: effect of presentation order on recall order
Provedor de dados: 56
Autores: Santos-Galduróz,R.F.; Oliveira,F.G.; Galduróz,J.C.F.; Bueno,O.F.A.
The influence of aging on memory has been extensively studied, but the importance of short-term memory and recall sequence has not. The objective of the current study was to examine the recall order of words presented on lists and to determine if age affects recall sequence. Physically and psychologically healthy male subjects were divided into two groups according to age, i.e., 23 young subjects (20 to 30 years) and 50 elderly subjects (60 to 70 years) submitted to the Wechsler Adult Intelligence Scale-Revised and the free word recall test. The order of word presentation significantly affected the 3rd and 4th words recalled (P &lt; 0.01; F = 14.6). In addition, there was interaction between the presentation order and the type of list presented (P &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recall order; Aging; Memory; Presentation order.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000019

Risk factors for excess weight loss and hypernatremia in exclusively breast-fed infants
Provedor de dados: 56
Autores: Çaglar,M.K.; Özer,I.; Altugan,F.S.
Data were prospectively obtained from exclusively breast-fed healthy term neonates at birth and from healthy mothers with no obstetric complication to determine risk factors for excess weight loss and hypernatremia in exclusively breast-fed infants. Thirty-four neonates with a weight loss  &gt; or = 10% were diagnosed between April 2001 and January 2005. Six of 18 infants who were eligible for the study had hypernatremia. Breast conditions associated with breast-feeding difficulties (P &lt; 0.05), primiparity (P &lt; 0.005), less than four stools (P &lt; 0.001), pink diaper (P &lt; 0.001), delay at initiation of first breast giving (P &lt; 0.01), birth by cesarean section (P &lt; 0.05), extra heater usage (P &lt; 0.005), extra heater usage among mothers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neonate; Dehydration; Breast-feeding; Hypernatremia; Weight loss.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400015

Inflammation and oxidative stress in heart failure: effects of exercise intensity and duration
Provedor de dados: 56
Autores: Ribeiro-Samora,G.A.; Rabelo,L.A.; Ferreira,A.C.C.; Favero,M.; Guedes,G.S.; Pereira,L.S.M.; Parreira,V.F.; Britto,R.R.
Although acute exercise is apparently pro-inflammatory and increases oxidative stress, it can promote the necessary stress stimulus to train chronic adaptations in patients with chronic heart failure (CHF). This study aimed to compare the effects of exercise intensity and duration on the inflammatory markers soluble tumor necrosis factor receptor (sTNFR1) and interleukin-6 (IL-6), and on oxidative stress [malondialdehyde (MDA) and antioxidant enzymes: catalase (CAT) and superoxide dismutase (SOD)] in individuals with CHF. Eighteen patients performed three exercise sessions: 30 min of moderate-intensity (M30) exercise, 30 min of low-intensity (L30) exercise, and 45 min of low-intensity (L45) exercise. Blood analysis was performed before exercise (baseline),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Inflammation; Oxidative stress; Exercise intensity; Exercise duration.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900608

Blockade of NK-1 receptors in the lateral commissural nucleus tractus solitarii of awake rats had no effect on the cardiovascular responses to chemoreflex activation
Provedor de dados: 56
Autores: Zhang,C.; Bonagamba,L.G.H.; Machado,B.H.
The neurotransmission of the chemoreflex in the nucleus tractus solitarii (NTS), particularly of the sympatho-excitatory component, is not completely understood. There is evidence that substance P may play a role in the neurotransmission of the chemoreflex in the NTS. Microinjection of substance P (50 pmol/50 nl, N = 12, and 5 nmol/50 nl, N = 8) into the commissural NTS of unanesthetized rats produced a significant increase in mean arterial pressure (101 ± 1 vs 108 ± 2 and 107 ± 3 vs 115 ± 4 mmHg, respectively) and no significant changes in heart rate (328 ± 11 vs 347 ± 15 and 332 ± 7 vs 349 ± 13 bpm, respectively) 2 min after microinjection. Previous treatment with WIN, an NK-1 receptor antagonist (2.5 nmol/50 nl), microinjected into the NTS of a specific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tachykinins; Cardiovascular regulation; Carotid chemoreceptors; Autonomic regulation; NK-1 receptors; Substance P.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100018

Changes in epidemiology of rotavirus in the Triângulo Mineiro region of Brazil: lack of two consecutive rotavirus seasons
Provedor de dados: 56
Autores: Dulgheroff,A.C.B.; Figueiredo,E.F.; Gouvêa,V.S.; Domingues,A.L.S.
Rotaviruses are the main cause of infantile acute diarrhea, and a monovalent (G1P[8]) vaccine against the virus was introduced into the Brazilian National Immunization Program for all infants in March 2006. The objectives of this study were to determine the rate and genotype distribution of rotavirus causing infantile diarrhea in the Triângulo Mineiro region of Brazil during 2011-2012 and to assess the impact of local vaccination. Fecal specimens were analyzed for detection and characterization of rotavirus using polyacrylamide gel electrophoresis, reverse transcription followed by polymerase chain reaction (PCR), and PCR-genotyping assays. Overall, rotavirus was diagnosed in 1.7% (6/348) of cases. Rotavirus positivity rates decreased 88% [95% confidence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Infantile diarrhea; Surveillance; Genotyping.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201091

Lack of evidence for regulation of cardiac P-type ATPases and MAP kinases in transgenic mice with cardiac-specific overexpression of constitutively active α1B-adrenoceptors
Provedor de dados: 56
Autores: Barreto,F.; Rezende,D.C.; Scaramello,C.B.V.; Silva,C.L.M.; Cunha,V.M.N.; Caricati-Neto,A.; Jurkiewicz,A.; Noël,F.; Quintas,L.E.M.
The regulatory function of α1B-adrenoceptors in mammalian heart homeostasis is controversial. The objective of the present study was to characterize the expression/activity of key proteins implicated in cardiac calcium handling (Na+/K+-ATPase and Ca2+-ATPases) and growth (ERK1/2, JNK1/2 and p38) in mice with cardiac-selective overexpression of constitutively active mutant α1B-adrenoceptor (CAMα1B-AR), which present a mild cardiac hypertrophy phenotype. Immunoblot assays showed that myocardial plasma membrane Ca2+-ATPase (PMCA) expression was increased by 30% in CAMα1B-AR mice (N = 6, P &lt; 0.05), although there was no change in sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2) expression. Moreover, total Ca2+-ATPase activity was not modified, but a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α1B-adrenoceptors; Ca2+-ATPases; Cardiac hypertrophy; Na+/K+-ATPase; Mitogen-activated protein kinases.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500012

Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)-based scaffolds for tissue engineering
Provedor de dados: 56
Autores: Chang,H.M.; Wang,Z.H.; Luo,H.N.; Xu,M.; Ren,X.Y.; Zheng,G.X.; Wu,B.J.; Zhang,X.H.; Lu,X.Y.; Chen,F.; Jing,X.H.; Wang,L.
Development and selection of an ideal scaffold is of importance for tissue engineering. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) is a biocompatible bioresorbable copolymer that belongs to the polyhydroxyalkanoate family. Because of its good biocompatibility, PHBHHx has been widely used as a cell scaffold for tissue engineering. This review focuses on the utilization of PHBHHx-based scaffolds in tissue engineering. Advances in the preparation, modification, and application of PHBHHx scaffolds are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PHBHHx; Tissue engineering; Biomaterial.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700533

Xanthine oxidase activity in patients with age-related cataract associated with hypertension
Provedor de dados: 56
Autores: Kisic,B.; Miric,D.; Zoric,L.; Rasic,J.V.; Grbic,R.; Popovic,Lj.M.; Arsic,A.M.
Reactive oxygen species and lipid peroxidation are important factors that contribute to the development of age-related cataract. The study included 130 patients with age-related cataract, 69 of whom were diagnosed with hypertension (HT), 20 with hypertension and type 2 diabetes mellitus (DM), and 41 had no accompanying condition. The following parameters were measured in the serum of the examinees: products of lipid peroxidation malondialdehyde (MDA) and lipofuscin-like fluorophores (LLF), activity of prooxidative enzymes xanthine oxidase (XO) and myeloperoxidase (MPO), antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), the concentration of thiol groups, and the ferric reducing activity of plasma. The activity of prooxidative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Age-related cataract; Hypertension; Xanthine oxidase; Myeloperoxidase.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500611

Prevalence of diphtheria and tetanus antibodies and circulation of Corynebacterium diphtheriae in São Paulo, Brazil
Provedor de dados: 56
Autores: Divino-Goes,K.G.; Moraes-Pinto,M.I. de; Dinelli,M.I.S.; Casagrande,S.T.; Bonetti,T.C.S.; Andrade,P.R.; Weckx,L.Y.
The introduction of routine vaccination against tetanus and diphtheria in Brazil has decreased the incidence and changed the epidemiology of both diseases. We then investigated the prevalence of Corynebacterium diphtheriae carrier status and diphtheria and tetanus immunity in São Paulo, Brazil. From November 2001 to March 2003, 374 individuals were tested for the presence of C. diphtheriae in the naso-oropharynx and of serum diphtheria and tetanus antibodies. Participants were all healthy individuals without acute or chronic pathologies and they were stratified by age as follows: 0-12 months and 1-4, 5-9, 10-14, 15-24, 25-39, 40-59, and ³60 years. Antibodies were assessed using a double-antigen ELISA. C. diphtheriae species were identified by biochemical...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diphtheria; Tetanus; Corynebacterium diphtheriae; Vaccine; Immunization; Antibody.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200012

Mapping cancer, cardiovascular and malaria research in Brazil
Provedor de dados: 56
Autores: Rodrigues,P.S.; Fonseca,L.; Chaimovich,H.
This paper presents performance indicators for the Brazilian cancer, cardiovascular and malaria research areas from 1981 to 1995. The data show an increasing number of papers since 1981 and author numbers indicate a continuous growth of the scientific community and suggest an expected impact of scientific activity on biomedical education. The data also characterize cardiovascular research as a well-established area and cancer research as a faster growing consolidating field. The 1989-1994 share of Brazilian articles among world publications shows a growing trend for the cancer (1.61) and cardiovascular (1.59) areas, and a decrease for the malaria area (0.89). The burden of the three diseases on society is contrasted by the small number of consolidated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Cardiovascular research; Malaria; Bibliometric indicators; Health indicators.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800001

Antigen-presenting cells transfected with Hsp65 messenger RNA fail to treat experimental tuberculosis
Provedor de dados: 56
Autores: Rocha,C.D.; Trombone,A.P.F.; Lorenzi,J.C.C.; Almeida,L.P.; Gembre,A.F.; Padilha,E.; Ramos,S.G.; Silva,C.L.; Coelho-Castelo,A.A.M.
In the last several years, the use of dendritic cells has been studied as a therapeutic strategy against tumors. Dendritic cells can be pulsed with peptides or full-length protein, or they can be transfected with DNA or RNA. However, comparative studies suggest that transfecting dendritic cells with messenger RNA (mRNA) is superior to other antigen-loading techniques in generating immunocompetent dendritic cells. In the present study, we evaluated a new therapeutic strategy to fight tuberculosis using dendritic cells and macrophages transfected with Hsp65 mRNA. First, we demonstrated that antigen-presenting cells transfected with Hsp65 mRNA exhibit a higher level of expression of co-stimulatory molecules, suggesting that Hsp65 mRNA has immunostimulatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Therapeutic immunization; CD; MHSP65; DNA vaccine; APCs.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200011

Expression of a hantavirus N protein and its efficacy as antigen in immune assays
Provedor de dados: 56
Autores: Figueiredo,L.T.M.; Moreli,M.L.; Borges,A.A.; Figueiredo,G.G.; Souza,R.L.M.; Aquino,V.H.
Hantavirus cardiopulmonary syndrome (HCPS) has been recognized as an important public heath problem. Five hantaviruses associated with HCPS are currently known in Brazil: Juquitiba, Araraquara, Laguna Negra-like, Castelo dos Sonhos, and Anajatuba viruses. The laboratory diagnosis of HCPS is routinely carried out by the detection of anti-hantavirus IgM and/or IgG antibodies. The present study describes the expression of the N protein of a hantavirus detected in the blood sample of an HCPS patient. The entire S segment of the virus was amplified and found to be 1858 nucleotides long, with an open reading frame of 1287 nucleotides that encodes a protein of 429 amino acids. The nucleotide sequence described here showed a high identity with the N protein gene...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diagnosis; Hantavirus infection; Araraquara hantavirus; N protein.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700008

Early and late neuropathological features of meningoencephalitis associated with Maraba virus infection
Provedor de dados: 56
Autores: Maia-Farias,A.; Lima,C.M.; Freitas,P.S.L.; Diniz,D.G.; Rodrigues,A.P.D.; Quaresma,J.A.S.; Diniz,C.W. Picanço; Diniz,J.A.
Maraba virus is a member of the genus Vesiculovirus of the Rhabdoviridae family that was isolated in 1983 from sandflies captured in the municipality of Maraba, state of Pará, Amazônia, Brazil. Despite 30 years having passed since its isolation, little is known about the neuropathology induced by the Maraba virus. Accordingly, in this study the histopathological features, inflammatory glial changes, cytokine concentrations, and nitric oxide activity in the encephalon of adult mice subjected to Maraba virus nostril infection were evaluated. The results showed that 6 days after intranasal inoculation, severe neuropathological-associated disease signs appeared, including edema, necrosis and pyknosis of neurons, generalized congestion of encephalic vessels,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maraba virus; Vesiculovirus; Microglia; Astrocytes; Cytokines; Nitric oxide.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400610

Liposomal photosensitizers: potential platforms for anticancer photodynamic therapy
Provedor de dados: 56
Autores: Muehlmann,L.A.; Joanitti,G.A.; Silva,J.R.; Longo,J.P.F.; Azevedo,R.B.
Photodynamic therapy is a well-established and clinically approved treatment for several types of cancer. Antineoplastic photodynamic therapy is based on photosensitizers, i.e., drugs that absorb photons translating light energy into a chemical potential that damages tumor tissues. Despite the encouraging clinical results with the approved photosensitizers available today, the prolonged skin phototoxicity, poor selectivity for diseased tissues, hydrophobic nature, and extended retention in the host organism shown by these drugs have stimulated researchers to develop new formulations for photodynamic therapy. In this context, due to their amphiphilic characteristic (compatibility with both hydrophobic and hydrophilic substances), liposomes have proven to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic therapy; Liposome; Antineoplastic therapy; Cancer; Photosensitizer.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800001

Effect of TNF-α production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1-infected individuals
Provedor de dados: 56
Autores: Luna,T.; Santos,S.B.; Nascimento,M.; Porto,M.A.F.; Muniz,A.L.; Carvalho,E.M.; Jesus,A.R.
Human T lymphotropic virus type 1 (HTLV-1) is the causal agent of myelopathy/tropical spastic paraparesis (HAM/TSP), a disease mediated by the immune response. HTLV-1 induces a spontaneous proliferation and production of pro-inflammatory cytokines by T cells, and increasing interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) levels are potentially involved in tissue damage in diseases related to HTLV-1. This exaggerated immune response is also due to an inability of the natural regulatory mechanisms to down-modulate the immune response in this group of patients. TNF-α inhibitors reduce inflammation and have been shown to improve chronic inflammatory diseases in clinical trials. The aim of this study was to evaluate the ability of pentoxifylline,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HTLV-1; TNF-α inhibitors; Pentoxifylline; Immune response.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100009

Interruption of the blood-stage cycle of the malaria parasite, Plasmodium chabaudi, by protein tyrosine kinase inhibitors
Provedor de dados: 56
Autores: Gazarini,M.L.; Garcia,C.R.S.
Malaria is a devastating disease caused by a unicellular protozoan, Plasmodium, which affects 3.7 million people every year. Resistance of the parasite to classical treatments such as chloroquine requires the development of new drugs. To gain insight into the mechanisms that control Plasmodium cell cycle, we have examined the effects of kinase inhibitors on the blood-stage cycle of the rodent malaria parasite, Plasmodium chabaudi. In vitro incubation of red blood cells for 17 h at 37ºC with the inhibitors led to a decrease in the percent of infected cells, compared to control treatment, as follows: genistein (200 µM - 75%), staurosporine (1 µM - 58%), R03 (1 µM - 75%), and tyrphostins B44 (100 µM - 66%) and B46 (100 µM - 68%). All these treatments were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Malaria; Plasmodium chabaudi; Kinase inhibitors; Signal transduction; Genistein; Staurosporin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100003

Prevalence of retinopathy in Caucasian type 2 diabetic patients from the South of Brazil and relationship with clinical and metabolic factors
Provedor de dados: 56
Autores: Santos,K.G.; Tschiedel,B.; Schneider,J.R.; Souto,K.E.P.; Roisenberg,I.
Diabetic retinopathy (DR) is a sight-threatening chronic complication of diabetes mellitus and is the leading cause of acquired blindness in adults. In this cross-sectional study, we investigated the prevalence of and the factors associated with DR in an analysis of 210 consecutive and unrelated Brazilian Caucasians with type 2 diabetes mellitus. Retinopathy was evaluated by ophthalmoscopy and/or biomicroscopy through dilated pupils. The relationship between clinical and metabolic variables and the presence of DR was assessed by logistic regression analysis. DR was detected in 99 of the 210 patients (47%). In the univariate logistic regression analyses, male sex, duration of diabetes, body mass index, glycated hemoglobin, C-peptide, LDL cholesterol,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diabetic retinopathy; Type 2 diabetes mellitus; Risk factors; Caucasian subjects.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200010

Effect of aerobic training on EEG alpha asymmetry and depressive symptoms in the elderly: a 1-year follow-up study
Provedor de dados: 56
Autores: Deslandes,A.C.; Moraes,H.; Alves,H.; Pompeu,F.A.M.S.; Silveira,H.; Mouta,R.; Arcoverde,C.; Ribeiro,P.; Cagy,M.; Piedade,R.A.M.; Laks,J.; Coutinho,E.S.F.
The effect of physical exercise on the treatment of depressive elderly adults has not been investigated thus far in terms of changes in cortical hemispheric activity. The objective of the present study was to identify changes in depressive symptoms, quality of life, and cortical asymmetry produced by aerobic activity. Elderly subjects with a diagnosis of major depressive disorder (DSM-IV) were included. Twenty patients (70% females, 71 ± 3 years) were divided into an exercise group (pharmacological treatment plus aerobic training) and a control group (undergoing pharmacological treatment) in a quasi-experimental design. Pharmacological treatment was maintained stable throughout the study (antidepressants and anxiolytics). Subjects were evaluated by...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Alpha asymmetry; Quality of life; Depressive symptoms; Physical activity; Aerobic training; Major depression.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600010

Gender differences in vascular expression of endothelin and ET A/ET B receptors, but not in calcium handling mechanisms, in deoxycorticosterone acetate-salt hypertension
Provedor de dados: 56
Autores: David,F.L.; Montezano,A.C.I.; Rebouças,N.A.; Nigro,D.; Fortes,Z.B.; Carvalho,M.H.C.; Tostes,R.C.A.
We determined if the increased vascular responsiveness to endothelin-1 (ET-1) observed in male, but not in female, DOCA-salt rats is associated with differential vascular mRNA expression of ET-1 and/or ET A/ET B receptors or with functional differences in Ca2+ handling mechanisms by vascular myocytes. Uninephrectomized male and female Wistar rats received DOCA and drinking water containing NaCl/KCl. Control rats received vehicle and tap water. Blood pressure and contractile responses of endothelium-denuded aortic rings to agents which induce Ca2+ influx and/or its release from internal stores were measured using standard procedures. Expression of mRNA for ET-1 and ET A/ET B receptors was evaluated by RT-PCR after isolation of total cell RNA from both aorta...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DOCA-salt hypertension; Endothelin-1; Gender; Endothelin receptors; Calcium; Rats.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900006

Evaluation of the immune humoral response of Brazilian patients with Rubinstein-Taybi syndrome
Provedor de dados: 56
Autores: Sugayama,S.M.M.; Arslanian,C.; Sales,M.M.; Carneiro-Sampaio,M.
Rubinstein-Taybi syndrome (RTS) is a rare developmental disorder characterized by craniofacial dysmorphisms, broad thumbs and toes, mental and growth deficiency, and recurrent respiratory infections. RTS has been associated with CREBBP gene mutations, but EP300 gene mutations have recently been reported in 6 individuals. In the present study, the humoral immune response in 16 RTS patients with recurrent respiratory infections of possible bacterial etiology was evaluated. No significant differences between patients and 16 healthy controls were detected to explain the high susceptibility to respiratory infections: normal or elevated serum immunoglobulin levels, normal salivary IgA levels, and a good antibody response to both polysaccharide and protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rubinstein-Taybi syndrome; High IgM levels; B lymphocyte; Antibody response.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200011

Efficacy of geraniol but not of β-ionone or their combination for the chemoprevention of rat colon carcinogenesis
Provedor de dados: 56
Autores: Vieira,A.; Heidor,R.; Cardozo,M.T.; Scolastici,C.; Purgatto,E.; Shiga,T.M.; Barbisan,L.F.; Ong,T.P.; Moreno,F.S.
β-ionone (βI), a cyclic isoprenoid, and geraniol (GO), an acyclic monoterpene, represent a promising class of dietary chemopreventive agents against cancer, whose combination could result in synergistic anticarcinogenic effects. The chemopreventive activities of βI and GO were evaluated individually or in combination during colon carcinogenesis induced by dimethylhydrazine in 48 3-week-old male Wistar rats (12 per group) weighing 40-50 g. Animals were treated for 9 consecutive weeks with βI (16 mg/100 g body weight), GO (25 mg/100 g body weight), βI combined with GO or corn oil (control). Number of total aberrant crypt foci (ACF) and of ACF ≥4 crypts in the distal colon was significantly lower in the GO group (66 ± 13 and 9 ± 2, respectively) compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colon; Carcinogenesis; Chemoprevention; Β-ionone; Geraniol; Isoprenoid; Acyclic monoterpene.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600007

Noncrystalline uric acid inhibits proteoglycan and glycosaminoglycan synthesis in distal tubular epithelial cells (MDCK)
Provedor de dados: 56
Autores: Borges,F.T.; Dalboni,M.A.; Michelacci,Y.M.; Schor,N.
Hyperuricemia is associated with renal stones, not only consisting of uric acid (UrAc) but also of calcium oxalate (CaOx). Glycosaminoglycans (GAGs) are well-known inhibitors of growth and aggregation of CaOx crystals. We analyzed the effect of noncrystalline UrAc on GAG synthesis in tubular distal cells. MDCK (Madin-Darby canine kidney) cells were exposed to noncrystalline UrAc (80 µg/mL) for 24 h. GAGs were labeled metabolically and characterized by agarose gel electrophoresis. The expression of proteoglycans and cyclooxygenase 2 (COX-2) was assessed by real-time PCR. Necrosis, apoptosis and prostaglandin E2 (PGE2) were determined by acridine orange, HOESCHT 33346, and ELISA, respectively. CaOx crystal endocytosis was evaluated by flow cytometry....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Noncrystalline uric acid; Glycosaminoglycan; MDCK; Lipopolysaccharide; Apoptosis; Proteoglycan.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000006

Human dental pulp stem cell adhesion and detachment in polycaprolactone electrospun scaffolds under direct perfusion
Provedor de dados: 56
Autores: Paim,A.; Braghirolli,D.I.; Cardozo,N.S.M.; Pranke,P.; Tessaro,I.C.
Cell adhesion in three-dimensional scaffolds plays a key role in tissue development. However, stem cell behavior in electrospun scaffolds under perfusion is not fully understood. Thus, an investigation was made on the effect of flow rate and shear stress, adhesion time, and seeding density under direct perfusion in polycaprolactone electrospun scaffolds on human dental pulp stem cell detachment. Polycaprolactone scaffolds were electrospun using a solvent mixture of chloroform and methanol. The viable cell number was determined at each tested condition. Cell morphology was analyzed by confocal microscopy after various incubation times for static cell adhesion with a high seeding density. Scanning electron microscopy images were obtained before and after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell adhesion; Perfusion; Shear stress; Stem cell; Electrospun scaffolds.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500615

Negative correlation between phospholipase and esterase activity produced by Fusarium isolates
Provedor de dados: 56
Autores: Ishida,K.; Alviano,D.S.; Silva,B.G.; Guerra,C.R.; Costa,A.S.; Nucci,M.; Alviano,C.S.; Rozental,S.
Fusarium species have emerged as one of the more outstanding groups of clinically important filamentous fungi, causing localized and life-threatening invasive infections with high morbidity and mortality. The ability to produce different types of hydrolytic enzymes is thought to be an important virulence mechanism of fungal pathogens and could be associated with the environment of the microorganism. Here, we have measured the production of two distinct lipolytic enzymes, phospholipase and esterase, by sixteen Fusarium isolates recovered from the hospital environment, immunocompromised patients’ blood cultures, foot interdigital space scrapings from immunocompromised patients, and foot interdigital space scrapings from immunocompetent patients (4 isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusarium; Hospital isolates; Phospholipase; Esterase.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500006

Pancreatic nitric oxide and oxygen free radicals in the early stages of streptozotocin-induced diabetes mellitus in the rat
Provedor de dados: 56
Autores: González,E.; Roselló-Catafau,J.; Jawerbaum,A.; Sinner,D.; Pustovrh,C.; Vela,J.; White,V.; Xaus,C.; Peralta,C.; Gimeno,M.
The objective of the present study was to explore the regulatory mechanisms of free radicals during streptozotocin (STZ)-induced pancreatic damage, which may involve nitric oxide (NO) production as a modulator of cellular oxidative stress. Removal of oxygen species by incubating pancreatic tissues in the presence of polyethylene glycol-conjugated superoxide dismutase (PEG-SOD) (1 U/ml) produced a decrease in nitrite levels (42%) and NO synthase (NOS) activity (50%) in diabetic but not in control samples. When NO production was blocked by N G-monomethyl-L-arginine (L-NMMA) (600 µM), SOD activity increased (15.21 ± 1.23 vs 24.40 ± 2.01 U/mg dry weight). The increase was abolished when the NO donor, spermine nonoate, was added to the incubating medium (13.2 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptozotocin diabetes; Pancreas; Oxidative stress; Nitric oxide.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100012

The carotid body of the spontaneous insulin-dependent diabetic rat
Provedor de dados: 56
Autores: Clarke,J.A.; Daly,M. de B.; Ead,H.W.; Hennessy,E.M.
The carotid bodies from adult spontaneous insulin-dependent diabetic rats (strain BB/S) were perfusion-fixed at normal arterial blood pressure with 3% phosphate-buffered glutaraldehyde and compared with the organs from control rats (strain BB/Sc) prepared in the same way. Serial 5-µm sections were cut, stained, and using an interactive image analysis system, were analysed to determine the volumes of the carotid body and its vascular and extravascular compartments. There was no evidence of systemic arterial disease in the carotid stem arteries in either group of animals, and the microvasculature of the organs appeared normal by light microscopy. The volume of the carotid body was unchanged 3 months after the onset of diabetes but was increased at 6 months....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diabetic rat; Carotid body; Quantitative morphology; Vascular compartment.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100013

Animal models of prenatal immune challenge and their contribution to the study of schizophrenia: a systematic review
Provedor de dados: 56
Autores: Macêdo,D.S.; Araújo,D.P.; Sampaio,L.R.L.; Vasconcelos,S.M.M.; Sales,P.M.G.; Sousa,F.C.F.; Hallak,J.E.; Crippa,J.A.; Carvalho,A.F.
Prenatal immune challenge (PIC) in pregnant rodents produces offspring with abnormalities in behavior, histology, and gene expression that are reminiscent of schizophrenia and autism. Based on this, the goal of this article was to review the main contributions of PIC models, especially the one using the viral-mimetic particle polyriboinosinic-polyribocytidylic acid (poly-I:C), to the understanding of the etiology, biological basis and treatment of schizophrenia. This systematic review consisted of a search of available web databases (PubMed, SciELO, LILACS, PsycINFO, and ISI Web of Knowledge) for original studies published in the last 10 years (May 2001 to October 2011) concerning animal models of PIC, focusing on those using poly-I:C. The results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schizophrenia; Animal models; Neurodevelopment; Prenatal immune activation; Poly-I:C.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300001

Epidemiological characterization of resistance and PCR typing of Shigella flexneri and Shigella sonnei strains isolated from bacillary dysentery cases in Southeast Brazil
Provedor de dados: 56
Autores: Penatti,M.P.A.; Hollanda,L.M.; Nakazato,G.; Campos,T.A.; Lancellotti,M.; Angellini,M.; Brocchi,M.; Rocha,M.M.M.; Silveira,W. Dias da.
Shigella spp are Gram-negative, anaerobic facultative, non-motile, and non-sporulated bacilli of the Enterobacteriaceae family responsible for "Shigellosis" or bacillary dysentery, an important cause of worldwide morbidity and mortality. However, despite this, there are very few epidemiological studies about this bacterium in Brazil. We studied the antibiotic resistance profiles and the clonal structure of 60 Shigella strains (30 S. flexneri and 30 S. sonnei) isolated from shigellosis cases in different cities within the metropolitan area of Campinas, State of São Paulo, Brazil. We used the following well-characterized molecular techniques: enterobacterial repetitive intergenic consensus, repetitive extragenic palindromic, and double-repetitive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shigella flexneri; Shigella sonnei; Antimicrobial resistance; Enterobacterial repetitive intergenic consensus- polymerase chain reaction; Repetitive extragenic palindromic-polymerase chain reaction; Double-repetitive element-polymerase chain reaction.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200012

Effect of unsaturated fatty acids on myocardial performance, metabolism and morphology
Provedor de dados: 56
Autores: Pinotti,M.F.; Silva,M.D.P.; Sugizaki,M.M.; Diniz,Y.S.; Sant'Ana,L.S.; Aragon,F.F.; Padovani,C.R.; Novelli,E.L.B.; Cicogna,A.C.
Diets rich in saturated fatty acids are one of the most important causes of atherosclerosis in men, and have been replaced with diets rich in unsaturated fatty acids (UFA) for the prevention of this disorder. However, the effect of UFA on myocardial performance, metabolism and morphology has not been completely characterized. The objective of the present investigation was to evaluate the effects of a UFA-rich diet on cardiac muscle function, oxidative stress, and morphology. Sixty-day-old male Wistar rats were fed a control (N = 8) or a UFA-rich diet (N = 8) for 60 days. Myocardial performance was studied in isolated papillary muscle by isometric and isotonic contractions under basal conditions after calcium chloride (5.2 mM) and ß-adrenergic stimulation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatty acids; Cardiac function; Oxidative stress; Morphology; Catalase; Superoxide dismutase.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200019

The regulatory role of IL-6R in hepatitis B-associated fibrosis and cirrhosis
Provedor de dados: 56
Autores: Chen,Y.; Yang,S.; Peng,Y.; Yang,Z.
This study investigated the expression and regulation of IL-6R in hepatitis B-associated moderate hepatic fibrosis and cirrhosis. Liver tissues, peripheral blood monocytes (PBMs) and serum were collected from 26 hepatitis B patients with liver fibrosis and 35 hepatitis B patients with liver cirrhosis. The levels of Il-6r mRNA expression in these samples were examined by quantitative real-time PCR and IL-6R protein levels were analyzed by western blot and ELISA. MiRNAs that regulate IL-6R expression were predicted by bioinformatics analysis, and validated by dual luciferase reporter assay. Compared with the hepatic fibrosis group, IL-6R was significantly upregulated at both mRNA and protein levels in liver tissues, PBMs and serum samples from the hepatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiRNA-30b; IL-6 receptor; Chronic hepatitis B; Fibrosis; Cirrhosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100610

Endothelium-dependent and -independent hepatic artery vasodilatation is not impaired in a canine model of liver ischemia-reperfusion injury
Provedor de dados: 56
Autores: Miranda,L.C.; Viaro,F.; Ceneviva,R.; Evora,P.R.B.
We investigated whether hepatic artery endothelium may be the earliest site of injury consequent to liver ischemia and reperfusion. Twenty-four heartworm-free mongrel dogs of either sex exposed to liver ischemia/reperfusion in vivo were randomized into four experimental groups (N = 6): a) control, sham-operated dogs, b) dogs subjected to 60 min of ischemia, c) dogs subjected to 30 min of ischemia and 60 min of reperfusion, and d) animals subjected to 45 min of ischemia and 120 min of reperfusion. The nitric oxide endothelium-dependent relaxation of hepatic artery rings contracted with prostaglandin F2a and exposed to increasing concentrations of acetylcholine, calcium ionophore A23187, sodium fluoride, phospholipase-C, poly-L-arginine, isoproterenol, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelium; Hepatic artery; Nitric oxide; Liver; Ischemia/reperfusion injury.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600016

Risk factors for ovarian failure in patients with systemic lupus erythematosus
Provedor de dados: 56
Autores: Medeiros,M.M.C.; Silveira,V.A.L.; Menezes,A.P.T.; Carvalho,R.C.
The aim of the present study was to identify the risk factors for ovarian failure in patients with systemic lupus erythematosus. Seventy-one women aged 17 to 45 years with systemic lupus erythematosus were studied. Patients were interviewed and their medical records reviewed. Demographic characteristics, clinical and serologic profiles, and menstrual and obstetric histories were recorded. Disease activity was measured by the systemic lupus erythematosus disease activity index. Serum FSH, LH, estradiol, progesterone, TSH, prolactin, and antimicrosomal and antithyroglobulin antibodies were measured. Patients who developed ovarian failure were compared to those who did not. Ovarian failure occurred in 11 patients (15.5%) and nine had premature menopause...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovarian failure; Systemic lupus erythematosus; Risk factors; Cyclophosphamide; Premature menopause.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200008

Obesity and kidney disease: hidden consequences of the epidemic
Provedor de dados: 56
Autores: Kovesdy,C.P.; Furth,S.L.; Zoccali,C.
Obesity has become a worldwide epidemic and its prevalence has been projected to grow by 40% in the next decade. This increasing prevalence has implications for the risk of diabetes, cardiovascular disease and also for chronic kidney disease (CKD). A high body mass index is one of the strongest risk factors for new-onset CKD. In individuals affected by obesity, a compensatory hyperfiltration occurs to meet the heightened metabolic demands of the increased body weight. The increase in intraglomerular pressure can damage the kidneys and raise the risk of developing CKD in the long-term. The incidence of obesity-related glomerulopathy has increased ten-fold in recent years. Obesity has also been shown to be a risk factor for nephrolithiasis, and for a number...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Obesity; Chronic kidney disease; Nephrolithiasis; Kidney cancer; Prevention.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500101

Trends in adjuvant development for vaccines: DAMPs and PAMPs as potential new adjuvants
Provedor de dados: 56
Autores: Miyaji,E.N.; Carvalho,E.; Oliveira,M.L.S.; Raw,I.; Ho,P.L.
Aluminum salts have been widely used in vaccine formulations and, after their introduction more than 80 years ago, only few vaccine formulations using new adjuvants were developed in the last two decades. Recent advances in the understanding of how innate mechanisms influence the adaptive immunity opened up the possibility for the development of new adjuvants in a more rational design. The purpose of this review is to discuss the recent advances in this field regarding the attempts to determine the molecular basis and the general mechanisms underlying the development of new adjuvants, with particular emphasis on the activation of receptors of innate immune recognition. One can anticipate that the use of these novel adjuvants will also provide a window of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adjuvant; Vaccine; Innate immunity; DAMP; PAMP.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600003

A direct contact between astrocyte and vitreous body is possible in the rabbit eye due to discontinuities in the basement membrane of the retinal inner limiting membrane
Provedor de dados: 56
Autores: Haddad,A.; Salazar,J.J.; Laicine,E.M.; Ramírez,A.I.; Ramírez,J.M.; Triviño,A.
Different from most mammalian species, the optic nerve of the rabbit eye is initially formed inside the retina where myelination of the axons of the ganglion cells starts and vascularization occurs. Astrocytes are confined to these regions. The aforementioned nerve fibers known as medullated nerve fibers form two bundles that may be identified with the naked eye. The blood vessels run on the inner surface of these nerve fiber bundles (epivascularization) and, accordingly, the accompanying astrocytes lie mostly facing the vitreous body from which they are separated only by the inner limiting membrane of the retina. The arrangement of the astrocytes around blood vessels leads to the formation of structures known as glial tufts. Fragments (N = 3) or whole...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Astrocyte; Basement membrane; Inner limiting membrane; Vitreous body; Rabbit retina; Plasma membrane.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200007

Tissue hyaluronan expression, as reflected in the sputum of lung cancer patients, is an indicator of malignancy
Provedor de dados: 56
Autores: Rangel,M.P.; de Sá,V.K.; Martins,V.; Martins,J.R.M.; Parra,E.R.; Mendes,A.; Andrade,P.C.; Reis,R.M.; Longatto-Filho,A.; Oliveira,C.Z.; Takagaki,T.; Carraro,D.M.; Nader,H.B.; Capelozzi,V.L.
Hyaluronan (HA) shows promise for detecting cancerous change in pleural effusion and urine. However, there is uncertainty about the localization of HA in tumor tissue and its relationship with different histological types and other components of the extracellular matrix, such as angiogenesis. We evaluated the association between HA and degree of malignancy through expression in lung tumor tissue and sputum. Tumoral tissue had significantly increased HA compared to normal tissue. Strong HA staining intensity associated with cancer cells was significant in squamous cell carcinoma compared to adenocarcinoma and large cell carcinoma. A significant direct association was found between tumors with a high percentage of HA and MVD (microvessel density) in tumoral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyaluronic acid; Sputum; Lung cancer; Immunohistochemistry; ELISA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600557

A glycoprotein E gene-deleted bovine herpesvirus 1 as a candidate vaccine strain
Provedor de dados: 56
Autores: Weiss,M.; Brum,M.C.S.; Anziliero,D.; Weiblen,R.; Flores,E.F.
A bovine herpesvirus 1 (BoHV-1) defective in glycoprotein E (gE) was constructed from a Brazilian genital BoHV-1 isolate, by replacing the full gE coding region with the green fluorescent protein (GFP) gene for selection. Upon co-transfection of MDBK cells with genomic viral DNA plus the GFP-bearing gE-deletion plasmid, three fluorescent recombinant clones were obtained out of approximately 5000 viral plaques. Deletion of the gE gene and the presence of the GFP marker in the genome of recombinant viruses were confirmed by PCR. Despite forming smaller plaques, the BoHV-1△gE recombinants replicated in MDBK cells with similar kinetics and to similar titers to that of the parental virus (SV56/90), demonstrating that the gE deletion had no deleterious effects...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle pathogen; Immunization; Control; Differential vaccine.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900843

Glucagon-like peptide 1 improves insulin resistance in vitro through anti-inflammation of macrophages
Provedor de dados: 56
Autores: Guo,C.; Huang,T.; Chen,A.; Chen,X.; Wang,L.; Shen,F.; Gu,X.
Glucagon-like peptide 1 (GLP-1), a kind of gut hormone, is used in the treatment of type 2 diabetes (T2D). Emerging evidence indicates that GLP-1 has anti-inflammatory activity. Chronic inflammation in the adipose tissue of obese individuals is a cause of insulin resistance and T2D. We hypothesized that GLP-1 analogue therapy in patients with T2D could suppress the inflammatory response of macrophages, and therefore inhibit insulin resistance. Our results showed that GLP-1 agonist (exendin-4) not only attenuated macrophage infiltration, but also inhibited the macrophage secretion of inflammatory cytokines including TNF-β, IL-6, and IL-1β. Furthermore, we observed that lipopolysaccharide (LPS)-induced macrophage conditioned media could impair...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Glucagon-like peptide; Macrophage infiltration; Adipose inflammation; Insulin resistance.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200602

Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells
Provedor de dados: 56
Autores: Ribeiro-Dias,F.; Russo,M.; Nascimento,F.R.F.; Barbuto,J.A.M.; Timenetsky,J.; Jancar,S.
Macrophages are important components of natural immunity involved in inhibition of tumor growth and destruction of tumor cells. It is known that these cells can be activated for tumoricidal activity by lymphokines and bacterial products. We investigated whether YAC-1 tumor cells infected with Mycoplasma arginini stimulate nitric oxide (NO) release and macrophage cytotoxic activity. Thioglycollate-elicited macrophages from male BALB/c mice were co-cultured for 20 h with YAC-1 tumor cells infected or not with Mycoplasma arginini. The cytotoxic activity was evaluated by MTT assay and nitrite levels were determined with the Griess reagent. Thioglycollate-elicited macrophages co-cultured with noninfected YAC-1 cells showed low cytotoxic activity (34.7 ± 8.6%)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Macrophages; Cytotoxicity; Mycoplasma; Tumor cells; Nitric oxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100010

Response threshold to aversive stimuli in stimulated early protein-malnourished rats
Provedor de dados: 56
Two animal models of pain were used to study the effects of short-term protein malnutrition and environmental stimulation on the response threshold to aversive stimuli. Eighty male Wistar rats were used. Half of the pups were submitted to malnutrition by feeding their mothers a 6% protein diet from 0 to 21 days of age while the mothers of the other half (controls) were well nourished, receiving 16% protein. From 22 to 70 days all rats were fed commercial lab chow. Half of the animals in the malnourished and control groups were maintained under stimulating conditions, including a 3-min daily handling from 0 to 70 days and an enriched living cage after weaning. The other half was reared in a standard living cage. At 70 days, independent groups of rats were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Early protein malnutrition; Enriched environment; Shock threshold test; Tail-flick test.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300016

Association of polymorphisms at the ADIPOR1 regulatory region with type 2 diabetes and body mass index in a Brazilian population with European or African ancestry
Provedor de dados: 56
Autores: Yeh,E.; Kimura,L.; Errera,F.I.V.; Angeli,C.B.; Mingroni-Netto,R.C.; Silva,M.E.R.; Canani,L.H.S.; Passos-Bueno,M.R.
Association studies between ADIPOR1 genetic variants and predisposition to type 2 diabetes (DM2) have provided contradictory results. We determined if two single nucleotide polymorphisms (SNP c.-8503G&gt;A and SNP c.10225C&gt;G) in regulatory regions of ADIPOR1 in 567 Brazilian individuals of European (EA; N = 443) or African (AfA; N = 124) ancestry from rural (quilombo remnants; N = 439) and urban (N = 567) areas. We detected a significant effect of ethnicity on the distribution of the allelic frequencies of both SNPs in these populations (EA: -8503A = 0.27; AfA: -8503A = 0.16; P = 0.001 and EA: 10225G = 0.35; AfA: 10225G = 0.51; P &lt; 0.001). Neither of the polymorphisms were associated with DM2 in the case-control study in EA (SNP c.-8503G&gt;A: DM2...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Association study; Adiponectin receptors; Type 2 diabetes; Polymorphism for ancestry-admixture mapping; HDL cholesterol; Association of ADIPOR1 with DM2.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600005

Contrast sensitivity to angular frequency gratings is not higher than to Cartesian gratings
Provedor de dados: 56
Autores: Zana,Y.; Cavalcanti,A.C.G.T.
When contrast sensitivity functions to Cartesian and angular gratings were compared in previous studies the peak sensitivity to angular stimuli was reported to be 0.21 log units higher. In experiments carried out to repeat this result, we used the same two-alternative forced-choice paradigm, but improved experimental control and precision by increasing contrast resolution from 8 to 12 bits, increasing the screen refresh rate from 30 Hz interlaced to 85 Hz non-interlaced, linearizing the voltage-luminance relation, modulating luminance in frequencies that minimize pixel aliasing, and improving control of the subject's exposure to the stimuli. The contrast sensitivity functions to Cartesian and angular gratings were similar in form and peak sensitivity (2.4...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contrast sensitivity functions; Cartesian and polar coordinates; Radial and angular gratings.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200010

STIM1/Orai1-mediated store-operated Ca2+ entry: the tip of the iceberg
Provedor de dados: 56
Autores: Giachini,F.R.; Lima,V.V.; Hannan,J.L.; Carneiro,F.S.; Webb,R.C.; Tostes,R.C.
Highly efficient mechanisms regulate intracellular calcium (Ca2+) levels. The recent discovery of new components linking intracellular Ca2+ stores to plasma membrane Ca2+ entry channels has brought new insight into the understanding of Ca2+ homeostasis. Stromal interaction molecule 1 (STIM1) was identified as a Ca2+ sensor essential for Ca2+ store depletion-triggered Ca2+ influx. Orai1 was recognized as being an essential component for the Ca2+ release-activated Ca2+ (CRAC) channel. Together, these proteins participate in store-operated Ca2+ channel function. Defective regulation of intracellular Ca2+ is a hallmark of several diseases. In this review, we focus on Ca2+ regulation by the STIM1/Orai1 pathway and review evidence that implicates STIM1/Orai1 in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ca2+ release-activated Ca2+ channel; Sarcoplasmic reticulum; Intracellular Ca2+ store; Ca2+ influx.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100002

Rescuing axons from degeneration does not affect retinal ganglion cell death
Provedor de dados: 56
Autores: de Lima,S.; Mietto,B.S.; Paula,C.; Muniz,T.; Martinez,A.M.B.; Gardino,P.F.
After a traumatic injury to the central nervous system, the distal stumps of axons undergo Wallerian degeneration (WD), an event that comprises cytoskeleton and myelin breakdown, astrocytic gliosis, and overexpression of proteins that inhibit axonal regrowth. By contrast, injured neuronal cell bodies show features characteristic of attempts to initiate the regenerative process of elongating their axons. The main molecular event that leads to WD is an increase in the intracellular calcium concentration, which activates calpains, calcium-dependent proteases that degrade cytoskeleton proteins. The aim of our study was to investigate whether preventing axonal degeneration would impact the survival of retinal ganglion cells (RGCs) after crushing the optic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Optic nerve crush; Calpain inhibitor; Axon preservation; Axon degeneration; Retinal ganglion cells; Cell survival.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400705

Glutamine and glutamate as vital metabolites
Provedor de dados: 56
Autores: Newsholme,P.; Lima,M.M.R.; Procopio,J.; Pithon-Curi,T.C.; Doi,S.Q.; Bazotte,R.B.; Curi,R.
Glucose is widely accepted as the primary nutrient for the maintenance and promotion of cell function. This metabolite leads to production of ATP, NADPH and precursors for the synthesis of macromolecules such as nucleic acids and phospholipids. We propose that, in addition to glucose, the 5-carbon amino acids glutamine and glutamate should be considered to be equally important for maintenance and promotion of cell function. The functions of glutamine/glutamate are many, i.e., they are substrates for protein synthesis, anabolic precursors for muscle growth, they regulate acid-base balance in the kidney, they are substrates for ureagenesis in the liver and for hepatic and renal gluconeogenesis, they act as an oxidative fuel for the intestine and cells of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutamine; Glutamate; Glucose; Metabolism; Cell function.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200002

Anxiety and depression in parents of a Brazilian non-clinical sample of attention-deficit/ hyperactivity disorder (ADHD) students
Provedor de dados: 56
Autores: Segenreich,D.; Fortes,D.; Coutinho,G.; Pastura,G.; Mattos,P.
Higher prevalence rates of anxiety and depression have been reported in parents of children with attention-deficit/hyperactivity disorder (ADHD). The interaction between the burden of ADHD in offspring, a higher prevalence rate of this highly inherited disorder in parents, and comorbidities may explain this finding. Our objective was to investigate levels of ADHD, anxious and depressive symptomatology, and their relationship in parents of ADHD children from a non-clinical sample using a dimensional approach. The sample included 396 students enrolled in all eight grades of a public school who were screened for ADHD using the SNAP IV rating scale. Positive cases were confirmed through a semi-structured interview. Parents of all 26 ADHD students and 31 paired...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attention-deficit/hyperactivity disorder; ADHD; Anxiety; Depression.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500011

Hemodialysis improves endothelial venous function in end-stage renal disease
Provedor de dados: 56
Autores: Silva,A.M.V.; Signori,L.U.; Plentz,R.D.M.; Moreno Jr.,H.; Barros,E.; Belló-Klein,A.; Schaan,B.D.; Irigoyen,M.C.
The objective of the present study was to determine the acute effect of hemodialysis on endothelial venous function and oxidative stress. We studied 9 patients with end-stage renal disease (ESRD), 36.8 ± 3.0 years old, arterial pressure 133.8 ± 6.8/80.0 ± 5.0 mmHg, time on dialysis 55.0 ± 16.6 months, immediately before and after a hemodialysis session, and 10 healthy controls matched for age and gender. Endothelial function was assessed by the dorsal hand vein technique using graded local infusion of acetylcholine (endothelium-dependent venodilation, EDV) and sodium nitroprusside (endothelium-independent venodilation). Oxidative stress was evaluated by measuring protein oxidative damage (carbonyls) and antioxidant defense (total radical trapping...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: End-stage renal disease; Hemodialysis; Oxidative stress; Vascular endothelium.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600008

Comparison of different monoclonal antibodies against immunosuppressive proteins of Ascaris suum
Provedor de dados: 56
Autores: Oshiro,T.M.; Rafael,A.; Enobe,C.S.; Fernandes,I.; Macedo-Soares,M.F.
The extract of Ascaris suum suppresses the humoral and cellular immune responses to unrelated antigens in the mouse. In order to further characterize the suppressive components of A. suum, we produced specific monoclonal antibodies which can provide an important tool for the identification of these proteins. The A. suum immunosuppressive fractions isolated by gel filtration from an extract of adult worms were used to immunize BALB/c mice. Popliteal lymph node cells taken from the immunized animals were fused with SP2/O myeloma cells and the cloned hybrid cells obtained were screened to determine the specificity of secreted antibodies. Three monoclonal antibodies named MAIP-1, MAIP-2 and MAIP-3 were selected and were shown to react with different epitopes...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ascaris suum; Immunosuppression; Immunomodulation; Monoclonal antibody.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200009

Association study of AFF1 rs340630 polymorphism with genetic susceptibility to rheumatoid arthritis in Chinese population
Provedor de dados: 56
Autores: Sun,Qing-Qing; Hua,Dong-Jin; Huang,Si-Chao; Cen,Han; Zhou,Li; Shao,Song.
This study was performed to examine whether the AF4/FMR2 family, member 1 (AFF1) rs340630 polymorphism is involved in the genetic background of rheumatoid arthritis (RA) in a Chinese population. Two different study groups of RA patients and controls (328 RA patients and 449 healthy controls in the first study group; 232 RA patients and 313 controls in the second study group) were included in our study. Overall, there was no significant difference in either genotype (P=0.71 and 0.64 in the first and second study group, respectively) nor allele (in the first study group: A vs G, P=0.65, OR=1.05, 95%CI=0.85–1.29; in the second study group: G vs A, P=0.47, OR=1.10, 95%CI=0.86–1.40) frequencies of AFF1 rs340630 polymorphism between RA patients and controls. Our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheumatoid arthritis; AFF1; Polymorphism; Chinese.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700606

Cardiovascular control in experimental diabetes
Provedor de dados: 56
Autores: De Angelis,K.; Schaan,B.D.; Maeda,C.Y.; Dall'Ago,P.; Wichi,R.B.; Irigoyen,M.C.
Several studies have reported impairment in cardiovascular function and control in diabetes. The studies cited in this review were carried out from a few days up to 3 months after streptozotocin administration and were concerned with the control of the circulation. We observed that early changes (5 days) in blood pressure control by different peripheral receptors were maintained for several months. Moreover, the impairment of reflex responses observed after baroreceptor and chemoreceptor stimulation was probably related to changes in the efferent limb of the reflex arc (sympathetic and parasympathetic), but changes also in the central nervous system could not be excluded. Changes in renal sympathetic nerve activity during volume expansion were blunted in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental diabetes; Arterial pressure; Autonomic control; Baroreflex; Chemoreflex; Cardiopulmonary reflex; Exercise training.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900010

Swimming training prevents coronary endothelial dysfunction in ovariectomized spontaneously hypertensive rats
Provedor de dados: 56
Autores: Claudio,E.R.G.; Almeida,S.A.; Mengal,V.; Brasil,G.A.; Santuzzi,C.H.; Tiradentes,R.V.; Gouvea,S.A.; Bissoli,N.S.; Santos,R.L.; Abreu,G.R.
Estrogen deficiency and hypertension are considered major risk factors for the development of coronary heart disease. On the other hand, exercise training is considered an effective form to prevent and treat cardiovascular diseases. However, the effects of swimming training (SW) on coronary vascular reactivity in female ovariectomized hypertensive rats are not known. We aimed to evaluate the effects of SW on endothelium-dependent coronary vasodilation in ovariectomized hypertensive rats. Three-month old spontaneously hypertensive rats (SHR, n=50) were divided into four groups: sham (SH), sham plus swimming training (SSW), ovariectomized (OVX), and ovariectomized plus swimming training (OSW). The SW protocol (5 times/week, 60 min/day) was conducted for 8...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swimming training; Ovariectomy; Coronary reactivity; Oxidative stress; Antioxidant enzymes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100607

Visual communication stimulates reproduction in Nile tilapia, Oreochromis niloticus (L.)
Provedor de dados: 56
Autores: Castro,A.L.S.; Gonçalves-de-Freitas,E.; Volpato,G.L.; Oliveira,C.
Reproductive fish behavior is affected by male-female interactions that stimulate physiological responses such as hormonal release and gonad development. During male-female interactions, visual and chemical communication can modulate fish reproduction. The aim of the present study was to test the effect of visual and chemical male-female interaction on the gonad development and reproductive behavior of the cichlid fish Nile tilapia, Oreochromis niloticus (L.). Fifty-six pairs were studied after being maintained for 5 days under one of the four conditions (N = 14 for each condition): 1) visual contact (V); 2) chemical contact (Ch); 3) chemical and visual contact (Ch+V); 4) no sensory contact (Iso) - males and females isolated. We compared the reproductive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Courtship; Cichlid; Gonadosomatic index; Visual cues; Chemical cues.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400009

Ectopic development of skeletal muscle induced by subcutaneous transplant of rat satellite cells
Provedor de dados: 56
Autores: Fukushima,M.G.; Furlan,I.; Chiavegatti,T.; Kiyomoto,B.H.; Godinho,R.O.
The present study analyzes the ectopic development of the rat skeletal muscle originated from transplanted satellite cells. Satellite cells (10(6) cells) obtained from hindlimb muscles of newborn female 2BAW Wistar rats were injected subcutaneously into the dorsal area of adult male rats. After 3, 7, and 14 days, the transplanted tissues (N = 4-5) were processed for histochemical analysis of peripheral nerves, inactive X-chromosome and acetylcholinesterase. Nicotinic acetylcholine receptors (nAChRs) were also labeled with tetramethylrhodamine-labeled alpha-bungarotoxin. The development of ectopic muscles was successful in 86% of the implantation sites. By day 3, the transplanted cells were organized as multinucleated fibers containing multiple clusters of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Skeletal muscle; Satellite cells; Stem cells; Transplantation; Myogenesis; 2BAW rats.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300007

Chronic imipramine treatment-induced changes in acetylcholinesterase (EC 3.1.1.7) activity in discrete rat brain regions
Provedor de dados: 56
Autores: Camarini,R.; Benedito,M.A.C.
Cholinergic as well as monoaminergic neurotransmission seems to be involved in the etiology of affective disorders. Chronic treatment with imipramine, a classical antidepressant drug, induces adaptive changes in monoaminergic neurotransmission. In order to identify possible changes in cholinergic neurotransmission we measured total, membrane-bound and soluble acetylcholinesterase (Achase) activity in several rat brain regions after chronic imipramine treatment. Changes in Achase activity would indicate alterations in acetylcholine (Ach) availability to bind to its receptors in the synaptic cleft. Male rats were treated with imipramine (20 mg/kg, ip) for 21 days, once a day. Twenty-four hours after the last dose the rats were sacrificed and homogenates from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylcholinesterase; Imipramine; Brain; Antidepressants.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800007

Short-term evaluation of non-absorbable microgranular hydroxyapatite infiltration in the guinea pig subepidermal abdominal region
Provedor de dados: 56
Autores: Xavier,A.P.; Schellini,S.A.; Aragon,F.F.; Padovani,C.R.; Taga,E.M.
Non-absorbable microgranular hydroxyapatite was infiltrated into the subepidermal abdominal region of guinea pigs in order to assess the possibility of using this material to correct deficiencies in orbital volume. Microgranular hydroxyapatite (2.0 ml) was subepidermally infiltrated into the abdominal region of 20 guinea pigs. The animals were divided into four experimental groups of 5 animals each, which were killed 7 (G1), 15 (G2), 30 (G3) and 60 (G4) days after infiltration. The area and the largest and smallest diameters of the nodules formed by infiltration were evaluated at the site of infiltration and histological examination was performed. The mean granuloma area was similar in all groups. Histopathological examination showed that the material...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infiltration; Microgranular hydroxyapatite; Guinea pigs; Histopathology.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100010

Effect of a cholesterol-rich diet on the metabolism of the free and esterified cholesterol components of a nanoemulsion that resembles LDL in rabbits
Provedor de dados: 56
Autores: Padoveze,A.F.; Maniero,F.; Oliveira,T.V.; Vitorio,T.S.; Couto,R.D.; Maranhão,R.C.
We have shown that the free cholesterol (FC) and the cholesteryl ester (CE) moieties of a nanoemulsion with lipidic structure resembling low-density lipoproteins show distinct metabolic fate in subjects and that this may be related to the presence of dyslipidemia and atherosclerosis. The question was raised whether induction of hyperlipidemia and atherosclerosis in rabbits would affect the metabolic behavior of the two cholesterol forms. Male New Zealand rabbits aged 4-5 months were allocated to a control group (N = 17) fed regular chow and to a 1% cholesterol-fed group (N = 13) during a 2-month period. Subsequently, the nanoemulsion labeled with ³H-FC and 14C-CE was injected intravenously for the determination of plasma kinetics and tissue uptake of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipoprotein kinetics; Free cholesterol; Hyperlipidemia; Rabbit atherosclerosis; Nanoemulsions; Cholesterol-rich diet.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200005

Metabolic fate of glutamine in lymphocytes, macrophages and neutrophils
Provedor de dados: 56
Autores: Curi,R.; Newsholme,P.; Pithon-Curi,T.C.; Pires-de-Melo,M.; Garcia,C.; Homem-de-Bittencourt Jr.,P.I.; Guimarães,A.R.P.
Eric Newsholme's laboratory was the first to show glutamine utilization by lymphocytes and macrophages. Recently, we have found that neutrophils also utilize glutamine. This amino acid has been shown to play a role in lymphocyte proliferation, cytokine production by lymphocytes and macrophages and phagocytosis and superoxide production by macrophages and neutrophils. Knowledge of the metabolic fate of glutamine in these cells is important for the understanding of the role and function of this amino acid in the maintenance of the proliferative, phagocytic and secretory capacities of these cells. Glutamine and glucose are poorly oxidized by these cells and might produce important precursors for DNA, RNA, protein and lipid synthesis. The high rate of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutamine; Lymphocytes; Macrophages; Neutrophils; Metabolic fate.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100002

Important amino acid residues of potato plant uncoupling protein (StUCP)
Provedor de dados: 56
Autores: Jezek,P.; Costa,A.D.T.; Vercesi,A.E.
Chemical modifications were used to identify some of the functionally important amino acid residues of the potato plant uncoupling protein (StUCP). The proton-dependent swelling of potato mitochondria in K+-acetate in the presence of linoleic acid and valinomycin was inhibited by mersalyl (Ki = 5 µM) and other hydrophilic SH reagents such as Thiolyte MB, iodoacetate and 5,5'-dithio-bis-(2-nitrobenzoate), but not by hydrophobic N-ethylmaleimide. This pattern of inhibition by SH reagents was similar to that of brown adipose tissue uncoupling protein (UCP1). As with UCP1, the arginine reagent 2,3-butadione, but not N-ethylmaleimide or other hydrophobic SH reagents, prevented the inhibition of StUCP-mediated transport by ATP in isolated potato mitochondria or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant mitochondria; Uncoupling protein; Chemical modification; Mitochondrial swelling; Reconstitution.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200003

Dose-dependent in vitro inhibition of rabbit corneal matrix metalloproteinases by an extract of Pothomorphe umbellata after alkali injury
Provedor de dados: 56
Autores: Barros,L.F.M.; Barros,P.S.M.; Röpke,C.D.; Silva,V.V.; Sawada,T.C.H.; Barros,S.B.M.; Belfort Jr.,R.
The in vitro ability of Pothomorphe umbellata ethanolic crude extract to inhibit matrix metalloproteinase (MMP) in normal cornea and in cornea after alkali injury was demonstrated. Corneas of albino rabbits were injured with 1 N NaOH for 20 s. After 48 h the corneas were excised, homogenized and analyzed for MMP-9 (92 kDa), pro-MMP-2 (72 kDa) and MMP-2 (67 kDa) activity by gelatin zymography. The activity was also measured in untreated corneas. After electrophoresis of 20 µg protein, gels were incubated with 50, 100, or 250 µg/mL lyophilized hydroethanolic (1:1) root crude extract of P. umbellata standardized for 4-nerolidylcatechol (7.09%). The activity of the enzymes was compared with that of untreated gel. At 48 h after injury, the activity of all MMPs...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cornea; Alkali injury; Metalloproteinases; Pothomorphe umbellata.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800014

Angiotensin II stimulates MCP-1 production in rat glomerular endothelial cells via NAD(P)H oxidase-dependent nuclear factor-kappa B signaling
Provedor de dados: 56
Autores: Pan,Q.; Yang,X.-H.; Cheng,Y.-X.
Angiotensin II (Ang II) plays a crucial role in the pathogenesis of renal diseases. The objective of the present study was to investigate the possible inflammatory effect of Ang II on glomerular endothelial cells and the underlying mechanism. We isolated and characterized primary cultures of rat glomerular endothelial cells (GECs) and observed that Ang II induced the synthesis of monocyte chemoattractant protein-1 (MCP-1) in GECs as demonstrated by Western blot. Ang II stimulation, at concentrations ranging from 0.1 to 10 µm, of rat GECs induced a rapid increase in the generation of reactive oxygen species as indicated by laser fluoroscopy. The level of p47phox protein, an NAD(P)H oxidase subunit, was also increased by Ang II treatment. These effects of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ang II; Glomerular endothelial cells; MCP-1; Reactive oxygen species; AT1 receptor blocker; NF-κB.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600009

Correlation between sodium and potassium excretion in 24- and 12-h urine samples
Provedor de dados: 56
Autores: Mill,J.G.; Silva,A.B.T. da; Baldo,M.P.; Molina,M.C.B.; Rodrigues,S.L.
Low-sodium and high-potassium diets have been recommended as an adjunct to prevention and treatment of hypertension. Analysis of these nutrients in 24-h urine has been considered the reference method to estimate daily intake of these minerals. However, 24-h urine collection is difficult in epidemiological studies, since urine must be collected and stored in job environments. Therefore, strategies for shorter durations of urine collection at home have been proposed. We have previously reported that collecting urine during a 12-h period (overnight) is more feasible and that creatinine clearance correlated strongly with that detected in 24-h samples. In the present study, we collected urine for 24 h divided into two 12-h periods (from 7:00 am to 7:00 pm and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sodium excretion; Urine; Salt intake; Potassium excretion.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900002

Cerebrospinal fluid-contacting nucleus mediates nociception via release of fractalkine
Provedor de dados: 56
Autores: Zhou,Q.Q.; Chen,S.S.; Zhang,Q.Q.; Liu,P.F.; Fang,H.Z.; Yang,Y.; Zhang,L.C.
Increasing evidence suggests that the cerebrospinal ﬂuid-contacting nucleus (CSF-contacting nucleus) mediates the transduction and regulation of pain signals. However, the precise molecular mechanisms remain unclear. Studies show that release of fractalkine (FKN) from neurons plays a critical role in nerve injury-related pain. We tested the hypothesis that release of FKN from the CSF-contacting nucleus regulates neuropathic pain, in a chronic constriction injury rat model. The results show that FKN is expressed by neurons, via expression of its only receptor CX3CR1 in the microglia. The levels of soluble FKN (sFKN) were markedly upregulated along with the increase in FKN mRNA level in rats subjected to chronic constriction injury. In addition, injection of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebrospinal fluid-contacting nucleus; FKN; Microglia; Neuropathic pain; Chronic constriction injury.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900610

Ambient particulate air pollution from vehicles promotes lipid peroxidation and inflammatory responses in rat lung
Provedor de dados: 56
Autores: Pereira,C.E.L.; Heck,T.G.; Saldiva,P.H.N.; Rhoden,C.R.
Oxidative stress plays a major role in the pathogenesis of particle-dependent lung injury. Ambient particle levels from vehicles have not been previously shown to cause oxidative stress to the lungs. The present study was conducted to a) determine whether short-term exposure to ambient levels of particulate air pollution from vehicles elicits inflammatory responses and lipid peroxidation in rat lungs, and b) determine if intermittent short-term exposures (every 4 days) induce some degree of tolerance. Three-month-old male Wistar rats were exposed to ambient particulate matter (PM) from vehicles (N = 30) for 6 or 20 continuous hours, or for intermittent (5 h) periods during 20 h for 4 consecutive days or to filtered air (PM &amp;lt;10 µm; N = 30). Rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Reactive oxygen species; Lipid peroxidation; Particulate air pollution; Lung inflammation; Acute effects.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000008

Streptococcus mutans GlnK protein: an unusual PII family member
Provedor de dados: 56
Autores: Portugal,M.E.G.; Souza,E.M.; Pedrosa,F.O.; Benelli,E.M.
Streptococcus mutans is a Gram-positive bacterium present in the oral cavity, and is considered to be one of the leading causes of dental caries. S. mutans has a glnK gene, which codes for a PII-like protein that is possibly involved in the integration of carbon, nitrogen and energy metabolism in several organisms. To characterize the GlnK protein of S. mutans, the glnK gene was amplified by PCR, and cloned into the expression vectors pET29a(+) and pET28b(+). The native GlnK-Sm was purified by anion exchange (Q-Sepharose) and affinity (Hi-Trap Heparin) chromatography. The GlnK-His-Sm protein was purified using a Hi-Trap Chelating-Ni2+ column. The molecular mass of the GlnK-His-Sm proteins was 85 kDa as determined by gel filtration, indicating that this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GlnK; Streptococcus mutans; Nitrogen metabolism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500003

Differential gene expression profiles of hepatocellular carcinomas associated or not with viral infection
Provedor de dados: 56
Autores: Bellodi-Privato,M.; Kubrusly,M.S.; Stefano,J.T.; Soares,I.C.; Wakamatsu,A.; Oliveira,A.C.; Alves,V.A.F.; Bacchella,T.; Machado,M.C.C.; D’Albuquerque,L.A.C.
Chronic hepatitis B (HBV) and C (HCV) virus infections are the most important factors associated with hepatocellular carcinoma (HCC), but tumor prognosis remains poor due to the lack of diagnostic biomarkers. In order to identify novel diagnostic markers and therapeutic targets, the gene expression profile associated with viral and non-viral HCC was assessed in 9 tumor samples by oligo-microarrays. The differentially expressed genes were examined using a z-score and KEGG pathway for the search of ontological biological processes. We selected a non-redundant set of 15 genes with the lowest P value for clustering samples into three groups using the non-supervised algorithm k-means. Fisher’s linear discriminant analysis was then applied in an exhaustive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; Molecular biomarkers; Viral infection; Oligo-microarrays.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200001

In vitro and in vivo antitumor activity of crude extracts obtained from Brazilian Chromobacterium sp isolates
Provedor de dados: 56
Autores: Menezes,C.B.A.; Silva,B.P.; Sousa,I.M.O.; Ruiz,A.L.T.G.; Spindola,H.M.; Cabral,E.; Eberlin,M.N.; Tinti,S.V.; Carvalho,J.E.; Foglio,M.A.; Fantinatti-Garboggini,F.
Natural products produced by microorganisms have been an important source of new substances and lead compounds for the pharmaceutical industry. Chromobacterium violaceum is a Gram-negative β-proteobacterium, abundant in water and soil in tropical and subtropical regions and it produces violacein, a pigment that has shown great pharmaceutical potential. Crude extracts of five Brazilian isolates of Chromobacterium sp (0.25, 2.5, 25, and 250 µg/mL) were evaluated in an in vitro antitumor activity assay with nine human tumor cells. Secondary metabolic profiles were analyzed by liquid chromatography and electrospray ionization mass spectrometry resulting in the identification of violacein in all extracts, whereas FK228 was detected only in EtCE 308 and EtCE 592...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromobacterium sp; Antitumor activity; Secondary metabolites; Genetic diversity; Violacein.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100065

Reduced plasma levels of angiotensin-(1-7) and renin activity in preeclamptic patients are associated with the angiotensin I- converting enzyme deletion/deletion genotype
Provedor de dados: 56
Autores: Velloso,E.P.; Vieira,R.; Cabral,A.C.; Kalapothakis,E; Santos,R.A.S.
The relationship between preeclampsia and the renin-angiotensin system (RAS) is poorly understood. Angiotensin I-converting enzyme (ACE) is a key RAS component and plays an important role in blood pressure homeostasis by generating angiotensin II (Ang II) and inactivating the vasodilator angiotensin-(1-7) (Ang-(1-7)). ACE (I/D) polymorphism is characterized by the insertion (I) or deletion (D) of a 287-bp fragment, leading to changes in ACE activity. In the present study, ACE (I/D) polymorphism was correlated with plasma Ang-(1-7) levels and several RAS components in both preeclamptic (N = 20) and normotensive pregnant women (N = 20). The percentage of the ACE DD genotype (60%) in the preeclamptic group was higher than that for the control group (35%);...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin-angiotensin system; Angiotensin-converting enzyme; Renin; Angiotensin-(1-7); ACE polymorphism (I/D); Preeclampsia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400018

Establishment of a rat model for uterine leiomyomas based on Western and traditional Chinese medicine theories
Provedor de dados: 56
Autores: Zhao,Hui; Li,Yao; Xu,QiuXia; Peng,Fu; Zhao,JinShuang; Webb,R. Clinton; Peng,Cheng; Yu,ChengHao.
Uterine leiomyomas (ULs) are benign monoclonal tumors that arise from the underlying myometrial tissue in the uterus. Effective therapies are still lacking because of poor understanding of the pathophysiology and epidemiology. Hence, it is urgent to establish efficient animal models to screen novel anti-UL therapies. In this study, for the first time, traditional Chinese medicine and Western medicine were combined to establish an animal model of ULs in rats. In order to evaluate the function and value of the novel model, it was compared with other models. The long-term and short-term rat models for ULs were established using progesterone and diethylstilbestrol. Rats in Qi stagnation and blood stasis group were injected with epinephrine hydrochloride and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uterine leiomyomas; Animal model; Traditional Chinese medicine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900603

Does hepatocellular carcinoma in non-alcoholic steatohepatitis exist in cirrhotic and non-cirrhotic patients?
Provedor de dados: 56
Autores: Chagas,A.L.; Kikuchi,L.O.O.; Oliveira,C.P.M.S.; Vezozzo,D.C.P.; Mello,E.S.; Oliveira,A.C.; Cella,L.C.; Herman,P.; Bachella,T.; Caldwell,S.H.; Alves,V.A.F.; Carrilho,F.J.
Non-alcoholic steatohepatitis (NASH) has been associated with hepatocellular carcinoma (HCC) often arising in histologically advanced disease when steatohepatitis is not active (cryptogenic cirrhosis). Our objective was to characterize patients with HCC and active, histologically defined steatohepatitis. Among 394 patients with HCC detected by ultrasound imaging over 8 years and staged by the Barcelona Clinic Liver Cancer (BCLC) criteria, we identified 7 cases (1.7%) with HCC occurring in the setting of active biopsy-proven NASH. All were negative for other liver diseases such as hepatitis C, hepatitis B, autoimmune hepatitis, Wilson disease, and hemochromatosis. The patients (4 males and 3 females, age 63 ± 13 years) were either overweight (4) or obese...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Hepatocellular carcinoma; Liver cancer; Fatty liver; Steatohepatitis; Cirrhosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000014

Astroglial cells derived from lateral and medial midbrain sectors differ in their synthesis and secretion of sulfated glycosaminoglycans
Provedor de dados: 56
Autores: Onofre,G.R.; Werneck,C.C.; Mendes,F.A.; Garcia-Abreu,J.; Moura Neto,V.; Cavalcante,L.A.; Silva,L.C.F.
Astroglial cells derived from lateral and medial midbrain sectors differ in their abilities to support neuritic growth of midbrain neurons in cocultures. These different properties of the two types of cells may be related to the composition of their extracellular matrix. We have studied the synthesis and secretion of sulfated glycosaminoglycans (GAGs) by the two cell types under control conditions and ß-D-xyloside-stimulated conditions, that stimulate the ability to synthesize and release GAGs. We have confirmed that both cell types synthesize and secrete heparan sulfate and chondroitin sulfate. Only slight differences were observed between the proportions of the two GAGs produced by the two types of cells after a 24-h labeling period. However, a marked...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycosaminoglycans; Astrocytes; Neuron-glia interactions; Midbrain.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200014

Pivotal role of leptin in insulin effects
Provedor de dados: 56
Autores: Ceddia,R.B.; William Jr.,W.N.; Lima,F.B.; Carpinelli,A.R.; Curi,R.
The OB protein, also known as leptin, is secreted by adipose tissue, circulates in the blood, probably bound to a family of binding proteins, and acts on central neural networks regulating ingestive behavior and energy balance. The two forms of leptin receptors (long and short forms) have been identified in various peripheral tissues, a fact that makes them possible target sites for a direct action of leptin. It has been shown that the OB protein interferes with insulin secretion from pancreatic islets, reduces insulin-stimulated glucose transport in adipocytes, and increases glucose transport, glycogen synthesis and fatty acid oxidation in skeletal muscle. Under normoglycemic and normoinsulinemic conditions, leptin seems to shift the flux of metabolites...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptin; Insulin secretion; Muscle metabolism; Insulin resistance; Adipocytes.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600001

Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly
Provedor de dados: 56
Autores: Mendes,G.A.; Haag,T.; Trott,G.; Rech,C.G.S.L.; Ferreira,N.P.; Oliveira,M.C.; Kohek,M.B.; Pereira-Lima,J.F.S.
Pituitary adenomas account for 10–15% of primary intracranial tumors. Growth hormone (GH)-secreting adenomas account for 13% of all pituitary adenomas and cause acromegaly. These tumors can be aggressive, invade surrounding structures and are highly recurrent. The objective of this study was to evaluate E-cadherin, Slug and neural cell adhesion molecule (NCAM) expression in GH-secreting pituitary adenomas and its relationship to tumor invasiveness. A cross–sectional study of patients who underwent hypophysectomy due to GH-secreting pituitary adenoma from April 2007 to December 2014 was carried out. The medical records were reviewed to collect clinical data. Immediately after surgery, tumor samples were frozen in liquid nitrogen and stored in a biofreezer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acromegaly; Pituitary neoplasms; Cadherins; Slug; Neural cell adhesion molecules.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200605

Development of the endocrine pancreas and novel strategies for β-cell mass restoration and diabetes therapy
Provedor de dados: 56
Autores: Márquez-Aguirre,A.L.; Canales-Aguirre,A.A.; Padilla-Camberos,E.; Esquivel-Solis,H.; Díaz-Martínez,N.E.
Diabetes mellitus represents a serious public health problem owing to its global prevalence in the last decade. The causes of this metabolic disease include dysfunction and/or insufficient number of β cells. Existing diabetes mellitus treatments do not reverse or control the disease. Therefore, β-cell mass restoration might be a promising treatment. Several restoration approaches have been developed: inducing the proliferation of remaining insulin-producing cells, de novo islet formation from pancreatic progenitor cells (neogenesis), and converting non-β cells within the pancreas to β cells (transdifferentiation) are the most direct, simple, and least invasive ways to increase β-cell mass. However, their clinical significance is yet to be determined....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endocrine pancreas; Diabetes mellitus; Β-cell regeneration; Β-cell replacement.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900765

CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells
Provedor de dados: 56
Autores: Mudado,M.A.; Rodrigues,A.L.; Prado,V.F.; Beirão,P.S.L.; Cruz,J.S.
T-type Ca2+ channels are important for cell signaling by a variety of cells. We report here the electrophysiological and molecular characteristics of the whole-cell Ca2+ current in GH3 clonal pituitary cells. The current inactivation at 0 mV was described by a single exponential function with a time constant of 18.32 ± 1.87 ms (N = 16). The I-V relationship measured with Ca2+ as a charge carrier was shifted to the left when we applied a conditioning pre-pulse of up to -120 mV, indicating that a low voltage-activated current may be present in GH3 cells. Transient currents were first activated at -50 mV and peaked around -20 mV. The half-maximal voltage activation and the slope factors for the two conditions are -35.02 ± 2.4 and 6.7 ± 0.3 mV (pre-pulse of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium channel; T-type; Electrophysiology; RT-PCR; GH3 cells.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600020

Synthesis, crystal structure, and anti-breast cancer activity of a novel metal-porphyrinic complex [YK(TCPP)(OH)2·(solvents)x]
Provedor de dados: 56
Autores: Liu,J-C.; Gu,J-J.; Zhang,Y-S.
A novel heterometallic metal-porphyrinic framework (MPFs) built from Y and K ions as nods and meso-tetra(4-carboxyphenyl)porphyrin as linkers has been successfully synthesized and characterized. The single crystal X-ray diffraction indicated that this complex 1 exhibited a bilayered architecture of the porphyrins, which is seldom seen in MPFs. In addition, in vitro anticancer activity of complex 1 on three human breast cancer cells (BT474, SKBr-3 and ZR-75-30) was further determined.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heterometallic; Single crystal; Breast cancer cell.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100611

Expression of beta 2 integrin (CD18) in embryonic mouse and chicken heart
Provedor de dados: 56
Autores: Oliveira,L.A.M.; Baker,R.K.; Klewer,S.E.; Kitten,G.T.
Integrins are heterodimeric receptors composed of α and β transmembrane subunits that mediate attachment of cells to the extracellular matrix and counter-ligands such as ICAM-1 on adjacent cells. β2 integrin (CD18) associates with four different α (CD11) subunits to form an integrin subfamily, which has been reported to be expressed exclusively on leukocytes. However, recent studies indicate that β2 integrin is also expressed by other types of cells. Since the gene for β2 integrin is located in the region of human chromosome 21 associated with congenital heart defects, we postulated that it may be expressed in the developing heart. Here, we show the results from several different techniques used to test this hypothesis. PCR analyses indicated that β2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beta 2 integrin; CD18; Cardiac development; Epithelial-mesenchymal transformation; Down syndrome; Mouse and chicken cardiogenesis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100005

Differential expression of AMPA-type glutamate receptor subunits during development of the chick optic tectum
Provedor de dados: 56
Autores: Batista,S.S.; Pires,R.S.; Britto,L.R.G.
Glutamate receptors have been often associated with developmental processes. We used immunohistochemical techniques to evaluate the expression of the AMPA-type glutamate receptor (GluR) subunits in the chick optic tectum (TeO). Chick embryos from the 5th through the 20th embryonic day (E5-E20) and one-day-old (P1) chicks were used. The three types of immunoreactivity evaluated (GluR1, GluR2/3, and GluR4) had different temporal and spatial expression patterns in the several layers of the TeO. The GluR1 subunit first appeared as moderate staining on E7 and then increased on E9. The mature GluR1 pattern included intense staining only in layer 5 of the TeO. The GluR2/3 subunits presented low expression on E5, which became intense on E7. The staining for...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: AMPA; Development; Glutamate receptors; Neurotransmitters; Receptor subunits; Visual system.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800015

Baicalin ameliorates chronic mild stress-induced depression-like behaviors in mice and attenuates inflammatory cytokines and oxidative stress
Provedor de dados: 56
Autores: Zhong,Juying; Li,Gonghua; Xu,Hong; Wang,Yan; Shi,Mingming.
The natural flavonoid glycoside baicalin (BA) produces a variety of pharmaceutical effects, particularly for psychiatric/neurological disorders. This study evaluated the behavioral and neuroprotective effects of BA in mice subjected to chronic unpredictable mild stress, a model of depression. BA (25 and 50 mg/kg) significantly increased sucrose consumption and reduced immobility times in the tail suspension and forced swim tests, demonstrating that BA alleviated depression-like behaviors. Moreover, BA reduced the levels of inflammatory cytokines, such as interleukin 1β, interleukin 6, and tumor necrosis factor α, in serum and in the hippocampus. BA also abrogated increases in NMDAR/NR2B and Ca2+/calmodulin-dependent protein kinase II, and the decrease in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baicalin; Depression; Neuroinflammatory; Oxidative stress.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700602

Factors associated with penicillin-nonsusceptible pneumococcal infections in Brazil
Provedor de dados: 56
Autores: Levin,A.S.; Sessegolo,J.F.; Teixeira,L.M.; Barone,A.A.
Resistance of Streptococcus pneumoniae is a worldwide, growing problem. Studies of factors associated with resistance to penicillin have not been conducted in Brazil. The objective of the present study was to evaluate factors associated with infection by S. pneumoniae not susceptible to penicillin. A prevalence study was conducted including all patients with a positive culture for S. pneumoniae in a hospital from July 1991 to December 1992 and the year 1994. Of 165 patients identified, 139 were considered to have clinically relevant infections and 88% of them had invasive infections. All infections were community acquired and consisted of pneumonia (44%) and of central nervous system (19%), pelvic or abdominal (12%), upper airway or ocular (12%), primary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Penicillin susceptibility; Risk factors; Prevalence study.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600017

Hepatitis C virus infection among Brazilian hemophiliacs: a virological, clinical and epidemiological study
Provedor de dados: 56
Autores: Carmo,R.A.; Oliveira,G.C.; Guimarães,M.D.C.; Oliveira,M.S.; Lima,A.A.; Buzek,S.C.; Corrêa-Oliveira,R.; Rocha,M.O.C.
We determined and analyzed risk factors of hepatitis C virus (HCV)-infected Brazilian hemophiliacs according to their virological, clinical and epidemiological characteristics. A cross-sectional and retrospective study of 469 hemophiliacs was carried out at a Brazilian blood center starting in October 1997. The prevalence of HCV infection, HCV genotypes and factors associated with HCV RNA detection was determined. The seroprevalence of anti-HCV antibodies (ELISA-3.0) was 44.6% (209/469). Virological, clinical and epidemiological assessments were completed for 162 positive patients. There were seven (4.3%) anti-HCV seroconversions between October 1992 and October 1997. During the same period, 40.8% of the positive anti-HCV hemophiliacs had abnormal alanine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C virus; HCV genotypes; Hemophilia; Chronic liver disease; Transfusion; Brazil.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500012

Transplantation of rat-derived microglial cells promotes functional recovery in a rat model of spinal cord injury
Provedor de dados: 56
Autores: Kou,Dewei; Li,Tianmi; Liu,Hong; Liu,Chuansheng; Yin,Yanwei; Wu,Xing; Yu,Tengbo.
This study evaluated the effect of microglia transplantation on neurological functional recovery in rats subjected to traumatic spinal cord injury (SCI). The rat model of SCI was established using a weight drop device. Forty SCI rats were randomly divided into the microglia group and the saline group. Then, rat-derived microglial cells or normal saline was injected into the injured site 7 days after surgery. The Basso-Beattie-Bresnahan (BBB) score, inclined plate test, and motor-evoked potentials (MEPs) were applied to assess the recovery of motor function. Hematoxylin and eosin (H&amp;E) staining was used to assess the therapeutic effect. Microglia transplantation significantly improved BBB scores and functional scores at 2, 3, 4, 6, and 8 weeks after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microglia; Spinal cord injury; Transplantation; CD68; OX42.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000603

Erythrocyte glucose-6-phosphate dehydrogenase activity assay and affinity for its substrate under "physiological" conditions
Provedor de dados: 56
Autores: Leite,A.A.; Barretto,O.C. de O.
Glucose-6-phosphate dehydrogenase (G6PD) activity and the affinity for its substrate glucose-6-phosphate were investigated under conditions similar to the physiological environment in terms of ionic strength (I: 0.188), cation concentration, pH 7.34, and temperature (37oC). A 12.4, 10.4 and 21.4% decrease was observed in G6PD B, G6PD A+ and G6PD A- activities, respectively. A Km increase of 95.1, 94.4 and 95.4% was observed in G6PD B, G6PD A+ and G6PD A-, respectively, leading to a marked decrease in affinity. In conclusion, the observation of the reduced activity and affinity for its natural substrate reflects the actual pentose pathway rate. It also suggests a much lower NADPH generation, which is crucial mostly in G6PD-deficient individuals, whose NADPH...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glucose-6-phosphate dehydrogenase; Red blood cells; G6PD activity and affinity.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200004

Chronic hypertension alters the expression of Cx43 in cardiovascular muscle cells
Provedor de dados: 56
Autores: Haefliger,J.-A.; Meda,P.
Connexin43 (Cx43), the predominant gap junction protein of muscle cells in vessels and heart, is involved in the control of cell-to-cell communication and is thought to modulate the contractility of the vascular wall and the electrical coupling of cardiac myocytes. We have investigated the effects of arterial hypertension on the expression of Cx43 in aorta and heart in three different models of experimental hypertension. Rats were made hypertensive either by clipping one renal artery (two kidney, one-clip renal (2K,1C) model) by administration of deoxycorticosterone and salt (DOCA-salt model) or by inhibiting nitric oxide synthase with NG-nitro-L-arginine methyl ester (L-NAME model). After 4 weeks, rats of the three models showed a similar increase in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell-cell communication; Gap junctions; Connexins; Vasculature; Heart; Hypertension; Blood pressure; Renin; Deoxycorticosterone; Nitric oxide; Distensibility.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400009

Fluorescent quantitative PCR of Mycobacterium tuberculosis for differentiating intestinal tuberculosis from Crohn's disease
Provedor de dados: 56
Autores: Fei,B.Y.; Lv,H.X.; Zheng,W.H.
Intestinal tuberculosis (ITB) and Crohn's disease (CD) are granulomatous disorders with similar clinical manifestations and pathological features that are often difficult to differentiate. This study evaluated the value of fluorescent quantitative polymerase chain reaction (FQ-PCR) for Mycobacterium tuberculosis (MTB) in fecal samples and biopsy specimens to differentiate ITB from CD. From June 2010 to March 2013, 86 consecutive patients (38 females and 48 males, median age 31.3 years) with provisional diagnoses of ITB and CD were recruited for the study. The patients' clinical, endoscopic, and histological features were monitored until the final definite diagnoses were made. DNA was extracted from 250 mg fecal samples and biopsy tissues from each patient....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal tuberculosis; Crohn's disease; Diagnosis; Fluorescent quantitative PCR; Mycobacterium tuberculosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200166

Regulation of the kinin receptors after induction of myocardial infarction: a mini-review
Provedor de dados: 56
Autores: Tschöpe,C.; Heringer-Walther,S.; Walther,T.
It is well known that the responses to vasoactive kinin peptides are mediated through the activation of two receptors termed bradykinin receptor B1 (B1R) and B2 (B2R). The physiologically prominent B2R subtype has certainly been the subject of more intensive efforts in structure-function studies and physiological investigations. However, the B1R activated by a class of kinin metabolites has emerged as an important subject of investigation within the study of the kallikrein-kinin system (KKS). Its inducible character under stress and tissue injury is therefore a field of major interest. Although the KKS has been associated with cardiovascular regulation since its discovery at the beginning of the last century, less is known about the B1R and B2R regulation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B1 receptor; B2 receptor; Bradykinin; Heart infarction; Kallikrein-kinin system.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600011

Yeast CUP1 protects HeLa cells against copper-induced stress
Provedor de dados: 56
Autores: Xie,X.X.; Ma,Y.F.; Wang,Q.S.; Chen,Z.L.; Liao,R.R.; Pan,Y.C.
As an essential trace element, copper can be toxic in mammalian cells when present in excess. Metallothioneins (MTs) are small, cysteine-rich proteins that avidly bind copper and thus play an important role in detoxification. Yeast CUP1 is a member of the MT gene family. The aim of this study was to determine whether yeast CUP1 could bind copper effectively and protect cells against copper stress. In this study, CUP1 expression was determined by quantitative real-time PCR, and copper content was detected by inductively coupled plasma mass spectrometry. Production of intracellular reactive oxygen species (ROS) was evaluated using the 2',7'-dichlorofluorescein-diacetate (DCFH-DA) assay. Cellular viability was detected using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast; Overexpression; Copper stress; Viability; ROS.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700616

Chlorpheniramine impairs functional recovery in Carassius auratus after telencephalic ablation
Provedor de dados: 56
Autores: Garção,D.C.; Canto-de-Souza,L.; Romaguera,F.; Mattioli,R.
We determined the effect of an H1 receptor antagonist on the functional recovery of Carassius auratus submitted to telencephalic ablation. Five days after surgery the fish underwent a spatial-choice learning paradigm test. The fish, weighing 6-12 g, were divided into four groups: telencephalic ablation (A) or sham lesion (S) and saline (SAL) or chlorpheniramine (CPA, ip, 16 mg/kg). For eight consecutive days each animal was trained individually in sessions separated by 24 h (alternate days). Training trials (T1-T8) consisted of finding the food in one of the feeders, which were randomly blocked for each subject. Animals received an intraperitoneal injection of SAL or CPA 10 min after the training trials. The time spent by the animals in each group to find...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional recovery; Spatial-choice learning; Histamine; Chlorpheniramine; Carassius auratus; Telencephalic ablation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400010

Influence of He-Ne laser therapy on the dynamics of wound healing in mice treated with anti-inflammatory drugs
Provedor de dados: 56
Autores: Gonçalves,W.L.S.; Souza,F.M.; Conti,C.L.; Cirqueira,J.P.; Rocha,W.A.; Pires,J.G.P.; Barros,L.A.P.; Moysés,M.R.
We determined the effects of helium-neon (He-Ne) laser irradiation on wound healing dynamics in mice treated with steroidal and non-steroidal anti-inflammatory agents. Male albino mice, 28-32 g, were randomized into 6 groups of 6 animals each: control (C), He-Ne laser (L), dexamethasone (D), D + L, celecoxib (X), and X + L. D and X were injected im at doses of 5 and 22 mg/kg, respectively, 24 h before the experiment. A 1-cm long surgical wound was made with a scalpel on the abdomens of the mice. Animals from groups L, D + L and X + L were exposed to 4 J (cm²)-1 day-1 of He-Ne laser for 12 s and were sacrificed on days 1, 2, or 3 after the procedure, when skin samples were taken for histological examination. A significant increase of collagen synthesis was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photo-stimulation; Skin; Collagen; Cyclooxygenase; Corticosteroids; Cicatrization; Anti-inflammatory drugs.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600018

Mutations in the HFE gene and sporadic amyotrophic lateral sclerosis risk: a meta-analysis of observational studies
Provedor de dados: 56
Autores: Li,M.; Wang,L.; Wang,W.; Qi,X.L.; Tang,Z.Y.
Iron homeostasis dysregulation has been regarded as an important mechanism in neurodegenerative diseases. The H63D and C282Y polymorphisms in theHFE gene may be involved in the development of sporadic amyotrophic lateral sclerosis (ALS) through the disruption of iron homeostasis. However, studies investigating the relationship between ALS and these two polymorphisms have yielded contradictory outcomes. We performed a meta-analysis to assess the roles of the H63D and C282Y polymorphisms of HFEin ALS susceptibility. PubMed, MEDLINE, EMBASE, and Cochrane Library databases were systematically searched to identify relevant studies. Strict selection criteria and exclusion criteria were applied. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amyotrophic lateral sclerosis; HFE; C282Y; H63D; Polymorphism; Meta-analysis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300215

Clinical assessment of the effect of digital filtering on the detection of ventricular late potentials
Provedor de dados: 56
Autores: Benchimol-Barbosa,P.R.; Barbosa,E.C.; Bomfim,A.S.; Ginefra,P.; Nadal,J.
Ventricular late potentials are low-amplitude signals originating from damaged myocardium and detected on the body surface by ECG filtering and averaging. Digital filters present in commercial equipment may interfere with the ability of arrhythmia stratification. We compared 40-Hz BiSpec (BI) and classical 40- to 250-Hz band-pass Butterworth bidirectional (BD) filters in terms of impact on time domain variables and diagnostic properties. In a transverse retrospective age-adjusted case-control study, 221 subjects with sinus rhythm without bundle branch block were divided into three groups after signal-averaged ECG acquisition: GI (N = 40), clinically normal controls, GII (N = 158), subjects with coronary heart disease without sustained monomorphic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High resolution electrocardiogram; Digital filtering; Health technology assessment.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100005

Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses
Provedor de dados: 56
Autores: Scandalios,J.G.
Molecular oxygen (O2) is the premier biological electron acceptor that serves vital roles in fundamental cellular functions. However, with the beneficial properties of O2 comes the inadvertent formation of reactive oxygen species (ROS) such as superoxide (O2&lt;FONT FACE=Symbol&gt;·-&lt;/FONT&gt;), hydrogen peroxide, and hydroxyl radical (OH&lt;FONT FACE=Symbol&gt;·&lt;/FONT&gt;). If unabated, ROS pose a serious threat to or cause the death of aerobic cells. To minimize the damaging effects of ROS, aerobic organisms evolved non-enzymatic and enzymatic antioxidant defenses. The latter include catalases, peroxidases, superoxide dismutases, and glutathione S-transferases (GST). Cellular ROS-sensing mechanisms are not well understood, but a number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalase; Aging; Telomeres; Gene regulation; Superoxide dismutase; Genomics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700003

Lithium carbonate and coenzyme Q10 reduce cell death in a cell model of Machado-Joseph disease
Provedor de dados: 56
Autores: Lopes-Ramos,C.M.; Pereira,T.C.; Dogini,D.B.; Gilioli,R.; Lopes-Cendes,I.
Machado-Joseph disease (MJD) or spinocerebellar ataxia type 3 (SCA3) is an autosomal dominant neurodegenerative disorder caused by expansion of the polyglutamine domain of the ataxin-3 (ATX3) protein. MJD/SCA3 is the most frequent autosomal dominant ataxia in many countries. The mechanism underlying MJD/SCA3 is thought to be mainly related to protein misfolding and aggregation leading to neuronal dysfunction followed by cell death. Currently, there are no effective treatments for patients with MJD/SCA3. Here, we report on the potential use of lithium carbonate and coenzyme Q10 to reduce cell death caused by the expanded ATX3 in cell culture. Cell viability and apoptosis were evaluated by MTT assay and by flow cytometry after staining with annexin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Machado-Joseph disease; Spinocerebellar ataxia type 3; Lithium carbonate; Coenzyme Q10; Drug treatment.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200601

Protein defects in neuromuscular diseases
Provedor de dados: 56
Autores: Vainzof,M.; Zatz,M.
Muscular dystrophies are a heterogeneous group of genetically determined progressive disorders of the muscle with a primary or predominant involvement of the pelvic or shoulder girdle musculature. The clinical course is highly variable, ranging from severe congenital forms with rapid progression to milder forms with later onset and a slower course. In recent years, several proteins from the sarcolemmal muscle membrane (dystrophin, sarcoglycans, dysferlin, caveolin-3), from the extracellular matrix (alpha2-laminin, collagen VI), from the sarcomere (telethonin, myotilin, titin, nebulin), from the muscle cytosol (calpain 3, TRIM32), from the nucleus (emerin, lamin A/C, survival motor neuron protein), and from the glycosylation pathway (fukutin,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscular dystrophies; Muscle proteins; Dystrophin; Sarcoglycans.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500001

Effect of repeated stress on novelty-induced antinociception in rats
Provedor de dados: 56
Autores: Torres,I.L.S.; Vasconcellos,A.P.; Silveira Cucco,S.N.; Dalmaz,C.
There is extensive evidence that acute stress induces an analgesic response in rats. On the other hand, repeatedly stressed animals may present the opposite effect, i.e., hyperalgesia. Furthermore, exposure to novelty is known to induce antinociception. The effects of repeated restraint stress on nociception after exposure to novelty, as measured by the tail-flick latency (TFL), were studied in adult male rats. The animals were stressed by restraint 1 h daily, 5 days a week for 40 days. The control group was not submitted to restraint. Nociception was assessed with a tail-flick apparatus. After being familiarized with the TFL apparatus, each group was subdivided into two other groups, i.e., with or without novelty. Animals were subjected to the TFL...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pain; Stress; Repeated stress; Nociception; Novelty.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200012

Effect of the time-of-day of training on explicit memory
Provedor de dados: 56
Autores: Barbosa,F.F.; Albuquerque,F.S.
Studies have shown a time-of-day of training effect on long-term explicit memory with a greater effect being shown in the afternoon than in the morning. However, these studies did not control the chronotype variable. Therefore, the purpose of this study was to assess if the time-of-day effect on explicit memory would continue if this variable were controlled, in addition to identifying the occurrence of a possible synchronic effect. A total of 68 undergraduates were classified as morning, intermediate, or afternoon types. The subjects listened to a list of 10 words during the training phase and immediately performed a recognition task, a procedure which they repeated twice. One week later, they underwent an unannounced recognition test. The target list and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Memory; Circadian rhythms; Explicit memory; Recognition memory.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600007

Effect of cyclooxygenase inhibitors on gentamicin-induced nephrotoxicity in rats
Provedor de dados: 56
Autores: Hosaka,E.M.; Santos,O.F.P.; Seguro,A.C.; Vattimo,M.F.F.
The frequent use of nonsteroidal anti-inflammatory drugs (NSAID) in combination with gentamicin poses the additional risk of nephrotoxic renal failure. Cyclooxygenase-1 (COX-1) is the main enzyme responsible for the synthesis of renal vasodilator prostaglandins, while COX-2 participates predominantly in the inflammatory process. Both are inhibited by non-selective NSAID such as indomethacin. Selective COX-2 inhibitors such as rofecoxib seem to have fewer renal side effects than non-selective inhibitors. The objective of the present study was to determine whether the combined use of rofecoxib and gentamicin can prevent the increased renal injury caused by gentamicin and indomethacin. Male Wistar rats (250-300 g) were treated with gentamicin (100 mg/kg body...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indomethacin; Rofecoxib; Nonsteroidal anti-inflammatory drugs; Aminoglycoside; Acute renal failure.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700006

Threats to bioethical principles in medical practice in Brazil: new medical ethics code period
Provedor de dados: 56
Autores: Gracindo,G.C.L.; da Silva Gallo,J.H.; Nunes,R.
We aimed to outline the profile of medical professionals in Brazil who have violated the deontological norms set forth in the ethics code of the profession, and whose cases were judged by the higher tribunal for medical ethics between 2010 and 2016. This survey was conducted using a database formed from professional ethics cases extracted from the plenary of the medical ethics tribunal of the Federal Council of Medicine. These were disciplinary ethics cases that were judged at appeal level between 2010 and 2016. Most of these professionals were male (88.5%) and their mean age was 59.9 years (SD=11.62) on the date of judgment of their appeals, ranging from 28 to 95 years. Most of them were based in the southeastern region of Brazil (50.89%). Articles 1 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioethics; Ethics codes; Medical ethics; Threats.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500606

Purification of bovine pancreatic glucagon as a by-product of insulin production
Provedor de dados: 56
Autores: Andrade,A.S.R.; Vilela,L.; Tunes,H.
A process for purifying bovine pancreatic glucagon as a by-product of insulin production is described. The glucagon-containing supernatant from the alkaline crystallization of insulin was precipitated using ammonium sulfate and isoelectric precipitation. The isoelectric precipitate containing glucagon was then purified by ion-exchange chromatography on Q-Sepharose FF, gel filtration on Sephadex G-25 and ion-exchange chromatography on S-Sepharose FF. A pilot scale test was performed with a recovery of 87.6% and a purification factor of 8.78 for the first chromatographic step, a recovery of 75.1% and a purification factor of 3.90 for the second, and a recovery of 76.2% and a purification factor of 2.36 for the last one. The overall yield was 50%, a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucagon; Protein purification; Gel filtration; Ion-exchange chromatography; Insulin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200006

Combating oncogene activation associated with retrovirus-mediated gene therapy of X-linked severe combined immunodeficiency
Provedor de dados: 56
Autores: Strauss,B.E.; Costanzi-Strauss,E.
A successful gene therapy clinical trial that also encountered serious adverse effects has sparked extensive study and debate about the future directions for retrovirus-mediated interventions. Treatment of X-linked severe combined immunodeficiency with an oncoretrovirus harboring a normal copy of the gc gene was applied in two clinical trials, essentially curing 13 of 16 infants, restoring a normal immune system without the need for additional immune-related therapies. Approximately 3 years after their gene therapy, tragically, 3 of these children, all from the same trial, developed leukemia as a result of this experimental treatment. The current understanding of the mechanism behind this leukemogenesis involves three critical and cooperating factors,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Retrovirus; Insertional mutagenesis; Severe combined immune deficiency; Gene therapy; Adverse event; Clinical trial.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500002

Development and applications of the SWAN rating scale for assessment of attention deficit hyperactivity disorder: a literature review
Provedor de dados: 56
Autores: Brites,C.; Salgado-Azoni,C.A.; Ferreira,T.L.; Lima,R.F.; Ciasca,S.M.
This study reviewed the use of the Strengths and Weaknesses of Attention-Deficit/Hyperactivity-symptoms and Normal-behaviors (SWAN) rating scale in diagnostic and evolutive approaches to attention deficit hyperactivity disorder (ADHD) and in correlational studies of the disorder. A review of articles published in indexed journals from electronic databases was conducted and 61 articles on the SWAN scale were analyzed. From these, 27 were selected to a) examine use of SWAN in research on attention disorders and b) verify evidence of its usefulness in the areas of genetics, neuropsychology, diagnostics, psychiatric comorbidities, neuroimaging, pharmacotherapy, and to examine its statistical reliability and validity in studies of diverse populations. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SWAN rating scale; Behavioral scale; Attention; Attention deficit hyperactivity disorder; Neuropsychology; Hyperactivity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001100965

Contrast sensitivity to radial frequencies modulated by Jn and j n Bessel profiles
Provedor de dados: 56
Autores: Simas,M.L.B.; Santos,N.A.
We measured human contrast sensitivity to radial frequencies modulated by cylindrical (Jo) and spherical (j o) Bessel profiles. We also measured responses to profiles of j o, j1, j2, j4, j8, and j16. Functions were measured three times by at least three of eight observers using a forced-choice method. The results conform to our expectations that sensitivity would be higher for cylindrical profiles. We also observed that contrast sensitivity is increased with the j n order for n greater than zero, having distinct orderly effects at the low and high frequency ends. For n = 0, 1, 2, and 4 sensitivity tended to occur around 0.8-1.0 cpd while for n = 8 and 16 it seemed to shift gradually to 0.8-3.0 cpd. We interpret these results as being consistent with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spatial frequency; Angular frequency; Polar gratings; Face perception; Contrast sensitivity.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100014

Thalidomide protects mice against LPS-induced shock
Provedor de dados: 56
Autores: Moreira,A.L.; Wang,J.; Sarno,E.N.; Kaplan,G.
Thalidomide has been shown to selectively inhibit TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; production in vitro by lipopolysaccharide (LPS)-stimulated monocytes. TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; has been shown to play a pivotal role in the pathophysiology of endotoxic shock. Using a mouse model of LPS-induced shock, we investigated the effects of thalidomide on the production of TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; and other cytokines and on animal survival. After injection of 100-350 µg LPS into mice, cytokines including TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;, IL-6, IL-10, IL-1ß, GM-CSF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endotoxic shock; Thalidomide; Cytokines; Inflammation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000010

Ovariectomy does not affect the cardiac sympathovagal balance of female SHR but estradiol does
Provedor de dados: 56
Autores: Dias,D.P.M.; Oliveira,M.; Salgado,H.C.; Fazan Jr.,R.
The low incidence of cardiovascular diseases, including hypertension, in premenopausal women has led to the conclusion that ovarian hormones may have a protective effect on the cardiovascular system. We evaluated the effects of ovariectomy and/or estradiol on sympathovagal balance and heart rate variability (HRV) in female spontaneously hypertensive rats (SHR) with tachycardia and compared them to Wistar rats (12 weeks old; N = 8-12). Ovariectomy (OVX) and/or estradiol (10 µg/kg) did not affect basal arterial pressure in either rat strain, but estradiol increased basal heart rate (HR) in OVX SHR (454 ± 18 vs 377 ± 9 bpm). HR changes elicited by methylatropine and propranolol were used to evaluate the sympathovagal balance. Ovariectomy did not affect the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estrogen; SHR; Heart rate variability; Hypertension; Sympathovagal balance.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000008

Cytokines as determinants of resistance and pathology in human Schistosoma mansoni infection
Provedor de dados: 56
Autores: Corrêa-Oliveira,R.; Malaquias,L.C.C.; Falcão,P.L.; Viana,I.R.C.; Bahia-Oliveira,L.M.G.; Silveira,A.M.S.; Fraga,L.A.O.; Prata,A.; Coffman,R.L.; Lambertucci,J.R.; Cunha-Melo,J.R.; Martins-Filho,O.A.; Wilson,R.A.; Gazzinelli,G.
The role of different cytokines in the peripheral blood mononuclear cell (PBMC) proliferative response and in in vitro granuloma formation was evaluated in a cross-sectional study with patients with the different clinical forms and phases of Schistosoma mansoni infection, as well as a group of individuals "naturally" resistant to infection named normal endemic (NE). The blockage of IL-4 and IL-5 using anti-IL-4 and anti-IL-5 antibodies significantly reduced the PBMC proliferative response to soluble egg (SEA) and adult worm (SWAP) antigens in acute (ACT), chronic intestinal (INT) and hepatosplenic (HS) patients. Similar results were obtained in the in vitro granuloma formation. Blockage of IL-10 had no significant effect on either assay using PBMC from ACT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosoma mansoni human schistosomiasis mansoni cytokines IL-10 IL-4 IFN-&lt; FONT FACE=Symbol&gt; G&lt; /font&gt.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100024

The anesthetic agent sevoflurane attenuates pulmonary acute lung injury by modulating apoptotic pathways
Provedor de dados: 56
Autores: Wang,L.; Ye,Y.; Su,H.B.; Yang,J.P.
The objective of this study was to evaluate lung protection by the volatile anesthetic sevoflurane (SEVO), which inhibits apoptosis. Male Sprague-Dawley rats (250–280 g; n=18) were randomly divided into three groups. The LPS group received 5 mg/kg endotoxin (lipopolysaccharide), which induced acute lung injury (ALI). The control (CTRL) group received normal saline and the SEVO group received sevoflurane (2.5%) for 30 min after ALI was induced by 5 mg/kg LPS. Samples were collected for analysis 12 h after LPS. Lung injury was assessed by pathological observations and tissue wet to dry weight (W/D) ratios. Apoptotic index (AI) was determined by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and electron microscopy. Caspase-3 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sevofluran; Apoptosis; Pulmonary acute lung injury; Caspase-3; Bcl-2.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300702

Behavioral and physiological methods for early quantitative assessment of spinal cord injury and prognosis in rats
Provedor de dados: 56
Autores: Giglio,C.A.; Defino,H.L.A.; da-Silva,C.A.; de-Souza,A.S.; Del Bel,E.A.
Methods for reliable evaluation of spinal cord (SC) injury in rats at short periods (2 and 24 h) after lesion were tested to characterize the mechanisms implicated in primary SC damage. We measured the physiological changes occurring after several procedures for producing SC injury, with particular emphasis on sensorimotor functions. Segmental and suprasegmental reflexes were tested in 39 male Wistar rats weighing 250-300 g divided into three control groups that were subjected to a) anesthesia, b) dissection of soft prevertebral tissue, and c) laminectomy of the vertebral segments between T10 and L1. In the lesion group the SC was completely transected, hemisected or subjected to vertebral compression. All animals were evaluated 2 and 24 h after the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trauma; Spinal cord injury; Experimental models; Sensorial test; Motor performance test.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200013

The methyl ester of rosuvastatin elicited an endothelium-independent and 3-hydroxy-3-methylglutaryl coenzyme A reductase-independent relaxant effect in rat aorta
Provedor de dados: 56
Autores: López-Canales,J.S.; López-Sanchez,P.; Perez-Alvarez,V.M.; Wens-Flores,I.; Polanco,A.C.; Castillo-Henkel,E.; Castillo-Henkel,C.
The relaxant effect of the methyl ester of rosuvastatin was evaluated on aortic rings from male Wistar rats (250-300 g, 6 rats for each experimental group) with and without endothelium precontracted with 1.0 µM phenylephrine. The methyl ester presented a slightly greater potency than rosuvastatin in relaxing aortic rings, with log IC50 values of -6.88 and -6.07 M, respectively. Unlike rosuvastatin, the effect of its methyl ester was endothelium-independent. Pretreatment with 10 µM indomethacin did not inhibit, and pretreatment with 1 mM mevalonate only modestly inhibited the relaxant effect of the methyl ester. Nω-nitro-L-arginine methyl ester (L-NAME, 10 µM), the selective nitric oxide-2 (NO-2) inhibitor 1400 W (10 µM), tetraethylammonium (TEA, 10 mM),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat aorta; Rosuvastatin methyl ester; Endothelium-independent relaxation; Potassium channels; NOS-2.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500009

Nuclear calcium signaling: a cell within a cell
Provedor de dados: 56
Autores: Rodrigues,M.A.; Gomes,D.A.; Nathanson,M.H.; Leite,M.F.
Calcium (Ca2+) is a versatile second messenger that regulates a wide range of cellular functions. Although it is not established how a single second messenger coordinates diverse effects within a cell, there is increasing evidence that the spatial patterns of Ca2+ signals may determine their specificity. Ca2+ signaling patterns can vary in different regions of the cell and Ca2+ signals in nuclear and cytoplasmic compartments have been reported to occur independently. No general paradigm has been established yet to explain whether, how, or when Ca2+ signals are initiated within the nucleus or their function. Here we highlight that receptor tyrosine kinases rapidly translocate to the nucleus. Ca2+ signals that are induced by growth factors result from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium; Proliferation; Receptor tyrosine kinases; Nucleus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100004

Association of terpinolene and diclofenac presents antinociceptive and anti-inflammatory synergistic effects in a model of chronic inflammation
Provedor de dados: 56
Autores: Macedo,E.M.A.; Santos,W.C.; Sousa Neto,B.P.; Lopes,E.M.; Piauilino,C.A.; Cunha,F.V.M.; Sousa,D.P.; Oliveira,F.A.; Almeida,F.R.C.
Pharmacological treatment of inflammatory pain is usually done by administration of non-steroidal anti-inflammatory drugs (NSAIDs). These drugs present high efficacy, although side effects are common, especially gastrointestinal lesions. One of the pharmacological strategies to minimize such effects is the combination of drugs and natural products with synergistic analgesic effect. The monoterpene terpinolene (TPL) is a chemical constituent of essential oils present in many plant species, which have pharmacological activities, such as analgesic and anti-inflammatory. The association of ineffective doses of TPL and diclofenac (DCF) (3.125 and 1.25 mg/kg po, respectively) presented antinociceptive and anti-inflammatory effects in the acute (0, 1, 2, 3, 4, 5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Terpinolene; Inflammation; Antinociception; Pharmacological synergism.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700602

Lack of evidence for monosomy 1p36 in patients with Prader-Willi-like phenotype
Provedor de dados: 56
Autores: Rodríguez,V.R.; Mazzucato,L.F.; Pina-Neto,J.M.
Monosomy 1p36 is the most common subtelomeric microdeletion syndrome with an incidence rate estimated to be 1 in 5000 births. A hypothesis of a similarity between patients with 1p36 deletion and those with Prader-Willi syndrome and the existence of two different phenotypes for 1p36 microdeletion has been suggested. The main objective of the present study was to determine the existence of 1p36 microdeletion in a sample of patients with mental retardation, obesity and hyperphagia who tested negative by the methylation test for Prader-Willi syndrome. Sixteen patients (7 females, 9 males), 16-26 years old, were evaluated with high-resolution cytogenetic analysis at 550-850 band levels and with 11 polymorphic microsatellite markers located in the 1p36 region....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Monosomy 1p36; 1p36 microdeletion syndrome; Prader-Willi syndrome; Prader-Willi-like phenotype.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800007

Evaluation of a 1-h 75-g oral glucose tolerance test in the diagnosis of gestational diabetes
Provedor de dados: 56
Autores: Campos,M.A.A.; Reichelt,A.A.J.; Façanha,C.; Forti,A.C.; Schmidt,M.I.
In order to evaluate the performance of a 1-h 75-g oral glucose tolerance test (OGTT) for the diagnosis of gestational diabetes mellitus (GDM), a cohort of 4998 women, 20 years or older, without previous diabetes being treated in prenatal care clinics in Brazil answered a questionnaire and performed a 75-g OGTT including fasting, 1-h and 2-h glucose measurements between their 24th and 28th gestational weeks. Pregnancy outcomes were transcribed from medical registries. GDM was defined according to WHO criteria (fasting: ≥126 mg/dL; 2-h value: ≥140 mg/dL) and macrosomia as a birth weight equal to or higher than 4000 g. Areas under the receiver operator characteristic curve (AUC) were compared and diagnostic properties of various cut-off points were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gestational diabetes; Diagnosis; Macrosomia; 1-h OGTT; Sensitivity; Specificity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800008

Immunophenotype of hematopoietic stem cells from placental/umbilical cord blood after culture
Provedor de dados: 56
Autores: Pranke,P.; Hendrikx,J.; Debnath,G.; Alespeiti,G.; Rubinstein,P.; Nardi,N.; Visser,J.
Identification and enumeration of human hematopoietic stem cells remain problematic, since in vitro and in vivo stem cell assays have different outcomes. We determined if the altered expression of adhesion molecules during stem cell expansion could be a reason for the discrepancy. CD34+CD38- and CD34+CD38+ cells from umbilical cord blood were analyzed before and after culture with thrombopoietin (TPO), FLT-3 ligand (FL) and kit ligand (KL; or stem cell factor) in different combinations: TPO + FL + KL, TPO + FL and TPO, at concentrations of 50 ng/mL each. Cells were immunophenotyped by four-color fluorescence using antibodies against CD11c, CD31, CD49e, CD61, CD62L, CD117, and HLA-DR. Low-density cord blood contained 1.4 ± 0.9% CD34+ cells, 2.6 ± 2.1% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematopoietic stem cells; CD34+CD38- cells; Human umbilical cord blood; Ex vivo expansion; Adhesion molecules.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200006

Autoimmune diseases in the TH17 era
Provedor de dados: 56
Autores: Mesquita Jr.,D.; Cruvinel,W.M.; Câmara,N.O.S.; Kállas,E.G.; Andrade,L.E.C.
A new subtype of CD4+ T lymphocytes characterized by the production of interleukin 17, i.e., TH17 cells, has been recently described. This novel T cell subset is distinct from type 1 and type 2 T helper cells. The major feature of this subpopulation is to generate significant amounts of pro-inflammatory cytokines, therefore appearing to be critically involved in protection against infection caused by extracellular microorganisms, and in the pathogenesis of autoimmune diseases and allergy. The dynamic balance among subsets of T cells is important for the modulation of several steps of the immune response. Disturbances in this balance may cause a shift from normal immunologic physiology to the development of immune-mediated disorders. In autoimmune diseases,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autoimmune diseases; Cytokines; TH17 cells; IL-17; IL-22; IL-23.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600002

Standard-curve competitive RT-PCR quantification of myogenic regulatory factors in chicken embryos
Provedor de dados: 56
Autores: Alvares,L.E.; Mantoani,A.; Corrente,J.E.; Coutinho,L.L.
The reverse transcription-polymerase chain reaction (RT-PCR) is the most sensitive method used to evaluate gene expression. Although many advances have been made since quantitative RT-PCR was first described, few reports deal with the mathematical bases of this technique. The aim of the present study was to develop and standardize a competitive PCR method using standard-curves to quantify transcripts of the myogenic regulatory factors MyoD, Myf-5, Myogenin and MRF4 in chicken embryos. Competitor cDNA molecules were constructed for each gene under study using deletion primers, which were designed to maintain the anchorage sites for the primers used to amplify target cDNAs. Standard-curves were prepared by co-amplification of different amounts of target cDNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quantitative RT-PCR; Standard-curve; Myogenic regulatory factors; Gene expression.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200004

Captopril alleviates hypertension-induced renal damage, inflammation, and NF-κB activation
Provedor de dados: 56
Autores: Gan,Zhongyuan; Huang,Dan; Jiang,Jiaye; Li,Yuan; Li,Hanqing; Ke,Yan.
Hypertensive renal damage generally occurs during the middle and late stages of hypertension, which is typically characterized by proteinuria and renal inflammation. Captopril, an angiotensin-converting enzyme (ACE) inhibitor, has been widely used for therapy of arterial hypertension and cardiovascular diseases. However, the protective effects of captopril on hypertension-induced organ damage remain elusive. The present study was designed to explore the renoprotective action of captopril in spontaneously hypertensive rats (SHR). The 6-week-old male SHR and age-matched Wistar-Kyoto rats were randomized into long-term captopril-treated (34 mg/kg) and vehicle-treated groups. The results showed that in SHR there was obvious renal injury characterized by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Captopril; Spontaneously hypertensive rats; Renal injury; Inflammation; Nuclear factor-κB.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100602

Patient and physician evaluation of the severity of acute asthma exacerbations
Provedor de dados: 56
Autores: Atta,J.A.; Nunes,M.P.T.; Fonseca-Guedes,C.H.F.; Avena,L.A.; Borgiani,M.T.; Fiorenza,R.F.; Martins,M.A.
We studied the ability of patients not experienced in the use of peak expiratory flow meters to assess the severity of their asthma exacerbations and compared it to the assessment of experienced clinicians. We also evaluated which data of physical examination and medical history are used by physicians to subjectively evaluate the severity of asthma attacks. Fifty-seven adult patients (15 men and 42 women, with a mean (± SD) age of 37.3 ± 14.5 years and 24.0 ± 17.9 years of asthma symptoms) with asthma exacerbations were evaluated in a University Hospital Emergency Department. Patients and physicians independently evaluated the severity of the asthma attack using a linear scale. Patient score, physician score and forced expiratory volume at the first second...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Asthma attack; Pulmonary function tests; Dyspnea.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900006

Role of Notch signaling in the mammalian heart
Provedor de dados: 56
Autores: Zhou,X.L.; Liu,J.C.
Notch signaling is an evolutionarily ancient, highly conserved pathway important for deciding cell fate, cellular development, differentiation, proliferation, apoptosis, adhesion, and epithelial-to-mesenchymal transition. Notch signaling is also critical in mammalian cardiogenesis, as mutations in this signaling pathway are linked to human congenital heart disease. Furthermore, Notch signaling can repair myocardial injury by promoting myocardial regeneration, protecting ischemic myocardium, inducing angiogenesis, and negatively regulating cardiac fibroblast-myofibroblast transformation. This review provides an update on the known roles of Notch signaling in the mammalian heart. The goal is to assist in developing strategies to influence Notch signaling and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Notch signaling; Congenital heart disease; Myocardial regeneration; Cardioprotection; Angiogenesis; Cardiac fibroblast-myofibroblast transformation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100001

Evidence that blood pressure remains under the control of arterial baroreceptors in renal hypertensive rats
Provedor de dados: 56
Autores: Trindade Jr.,A.S.; Moreira,E.D.; Silva,G.J.J.; Krieger,E.M.
The purpose of the present study was to determine the range of the influence of the baroreflex on blood pressure in chronic renal hypertensive rats. Supramaximal electrical stimulation of the aortic depressor nerve and section of the baroreceptor nerves (sinoaortic denervation) were used to obtain a global analysis of the baroreceptor-sympathetic reflex in normotensive control and in chronic (2 months) 1-kidney, 1-clip hypertensive rats. The fall in blood pressure produced by electrical baroreceptor stimulation was greater in renal hypertensive rats than in normotensive controls (right nerve: -47 ± 8 vs -23 ± 4 mmHg; left nerve: -51 ± 7 vs -30 ± 4 mmHg; and both right and left nerves: -50 ± 8 vs -30 ± 4 mmHg; P &lt; 0.05). Furthermore, the increase in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Arterial baroreceptor; Renal hypertension; Sinoaortic denervation; Aortic depressor nerve; Electrical stimul.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000013

Reduction of intraspecific aggression in adult rats by neonatal treatment with a selective serotonin reuptake inhibitor
Provedor de dados: 56
Autores: Manhães de Castro,R.; Barreto Medeiros,J.M.; Mendes da Silva,C.; Ferreira,L.M.P.; Guedes,R.C.A.; Cabral Filho,J.E.; Costa,J.A.
Most studies suggest that serotonin exerts an inhibitory control on the aggression process. According to experimental evidence, this amine also influences growth and development of the nervous tissue including serotoninergic neurons. Thus, the possibility exists that increased serotonin availability in young animals facilitates a long-lasting effect on aggressive responses. The present study aimed to investigate the aggressive behavior of adult rats (90-120 days) treated from the 1st to the 19th postnatal day with citalopram (CIT), a selective serotonin reuptake inhibitor (20 mg/kg, sc, every 3 days). Aggressive behavior was induced by placing a pair of rats (matched by weight) in a box (20 x 20 x 20 cm), and submitting them to a 20-min session of electric...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Aggression; Serotonin; Neonatal treatment; Development.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100015

Normal skin of HIV-infected individuals contains increased numbers of dermal CD8 T cells and normal numbers of Langerhans cells
Provedor de dados: 56
Autores: Galhardo,M.C.G.; Alvarenga,F.F.; Schueler,G.; Perez,M.; Morgado,M.G.; Ferreira,H.; Azevedo,L.M.S.; Sampaio,E.P.; Sarno,E.N.
Dysregulation of the skin immune system (SIS) could explain the high prevalence of skin disorders in HIV+ individuals. The present study was carried out to determine whether alterations in the cell population of SIS and epidermal immunoactivation occur in the normal skin of HIV+ individuals. Forty-five biopsies were taken from the normal upper arm skin of 45 HIV+ patients and of 15 healthy controls. HIV+ individuals were divided into three categories according to their CD4 cell blood count (&lt;200, 200-499 and ³500/µl). Hematoxylin-eosin was used to stain tissue sections for morphological analysis and immunohistochemistry was used for the evaluation of the frequency of macrophages, Langerhans cells, and CD lymphocyte subsets. In addition, semiquantitative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Skin immune system; CD8 T cells; CD4-CD8 ratio; Langerhans cells.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500017

Bone metabolism and vascular calcification
Provedor de dados: 56
Autores: Danilevicius,C.F.; Lopes,J.B.; Pereira,R.M.R.
Osteoporosis and atherosclerosis are chronic degenerative diseases which have been considered to be independent and whose common characteristic is increasing incidence with age. At present, growing evidence indicates the existence of a correlation between cardiovascular disease and osteoporosis, irrespective of age. The morbidity and mortality of osteoporosis is mainly related to the occurrence of fractures. Atherosclerosis shows a high rate of morbidity and especially mortality because of its clinical repercussions such as angina pectoris, acute myocardial infarction, stroke, and peripheral vascular insufficiency. Atherosclerotic disease is characterized by the accumulation of lipid material in the arterial wall resulting from autoimmune and inflammatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoporosis; Vascular calcification; Atherosclerosis; Low-bone mineral density; Arterial calcification.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400001

Association between the increase in brain temperature and physical performance at different exercise intensities and protocols in a temperate environment
Provedor de dados: 56
Autores: Kunstetter,A.C.; Wanner,S.P.; Madeira,L.G.; Wilke,C.F.; Rodrigues,L.O.C.; Lima,N.R.V.
There is evidence that brain temperature (Tbrain) provides a more sensitive index than other core body temperatures in determining physical performance. However, no study has addressed whether the association between performance and increases in Tbrain in a temperate environment is dependent upon exercise intensity, and this was the primary aim of the present study. Adult male Wistar rats were subjected to constant exercise at three different speeds (18, 21, and 24 m/min) until the onset of volitional fatigue. Tbrain was continuously measured by a thermistor inserted through a brain guide cannula. Exercise induced a speed-dependent increase in Tbrain, with the fastest speed associated with a higher rate of Tbrain increase. Rats subjected to constant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain cortex; Fatigue; Hyperthermia; Speed; Thermoregulation; Treadmill running.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800679

Low-cost automatic activity data recording system
Provedor de dados: 56
Autores: Moraes,M.F.D.; Ferrarezi,C.; Mont'Alverne,F.J.A.; Garcia-Cairasco,N.
We describe a low-cost, high quality device capable of monitoring indirect activity by detecting touch-release events on a conducting surface, i.e., the animal's cage cover. In addition to the detecting sensor itself, the system includes an IBM PC interface for prompt data storage. The hardware/software design, while serving for other purposes, is used to record the circadian activity rhythm pattern of rats with time in an automated computerized fashion using minimal cost computer equipment (IBM PC XT). Once the sensor detects a touch-release action of the rat in the upper portion of the cage, the interface sends a command to the PC which records the time (hours-minutes-seconds) when the activity occurred. As a result, the computer builds up several files...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circadian rhythm; Automated acquisition; Actograms; FFT; Cosinor; Chronobiology.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800015

Effect of laser treatment on postural control parameters in patients with chronic nonspecific low back pain: a randomized placebo-controlled trial
Provedor de dados: 56
Autores: Taradaj,J.; Rajfur,K.; Rajfur,J.; Ptaszkowski,K.; Ptaszkowska,L.; Sopel,M.; Rosińczuk,J.; Dymarek,R.
The management of nonspecific lumbar pain (NSLP) using laser irradiation remains controversial. A systematic review of recently published studies indicates that the effects of laser therapy are commonly assessed using only imperfect methods in terms of measurement error. The main objective of this study was to assess static postural stability using an objective tool in patients with chronic NSLP after laser irradiation at different doses and wavelengths. In total, 68 patients were included in the laser sessions and were randomly assigned into four groups: high-intensity laser therapy at 1064 nm and 60 J/cm2 for 10 min (HILT), sham (HILT placebo), low-level laser therapy at 785 nm and 8 J/cm2 for 8 min (LLLT), and sham (LLLT placebo). In addition, all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laser therapy; Low back pain; Physical therapy modalities; Postural balance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200605

Bradykinin enhances membrane electrical activity of pancreatic beta cells in the presence of low glucose concentrations
Provedor de dados: 56
Autores: Moura,A.S.
In most of cells bradykinin (BK) induces intracellular calcium mobilization. In pancreatic beta cells intracellular calcium is a major signal for insulin secretion. In these cells, glucose metabolism yields intracellular ATP which blocks membrane potassium channels. The membrane depolarizes, voltage-dependent Ca2+ channels are activated and the intracellular calcium load allows insulin secretion. Repolarization occurs due to activation of the Ca2+-dependent K+ channel. The insulin secretion depends on the integrity of this oscillatory process (bursts). Therefore, we decided to determine whether BK (100 nM) induces bursts in the presence of a non-stimulatory glucose concentration (5.6 mM). During continuous membrane voltage recording, our results showed...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Membrane potential; Pancreatic beta cell; Bradykinin; Calcium.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900016

ZapA, a possible virulence factor from Proteus mirabilis exhibits broad protease substrate specificity
Provedor de dados: 56
Autores: Anéas,M.A.F.; Portaro,F.C.V.; Lebrun,I.; Juliano,L.; Palma,M.S.; Fernandes,B.L.
The opportunistic bacterium Proteus mirabilis secretes a metalloprotease, ZapA, considered to be one of its virulence factors due to its IgA-degrading activity. However, the substrate specificity of this enzyme has not yet been fully characterized. In the present study we used fluorescent peptides derived from bioactive peptides and the oxidized ß-chain of insulin to determine the enzyme specificity. The bradykinin- and dynorphin-derived peptides were cleaved at the single bonds Phe-Ser and Phe-Leu, with catalytic efficiencies of 291 and 13 mM/s, respectively. Besides confirming already published cleavage sites, a novel cleavage site was determined for the ß-chain of insulin (Val-Asn). Both the natural and the recombinant enzyme displayed the same broad...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proteus mirabilis; Metalloprotease; Substrate specificity; Fluorogenic peptides; IgA; Insulin ß-chain.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100004

Short-term levosimendan treatment protects rat testes against oxidative stress
Provedor de dados: 56
Autores: Yuksel,M.B.; Kavak,S.; Gecit,I.; Basel,H.; Gümrükçüoğlu,H.A.; Demir,H.; Meral,İ.
The objective of this study was to evaluate the effect of short-term levosimendan exposure on oxidant/antioxidant status and trace element levels in the testes of rats under physiological conditions. Twenty male Wistar albino rats were randomly divided into two groups of 10 animals each. Group 1 was not exposed to levosimendan and served as control. Levosimendan (12 µg/kg) diluted in 10 mL 0.9% NaCl was administered intraperitoneally to group 2. Animals of both groups were sacrificed after 3 days and their testes were harvested for the determination of changes in tissue oxidant/antioxidant status and trace element levels. Tissue malondialdehyde (MDA) was significantly lower in the levosimendan group (P &lt; 0.001) than in the untreated control group and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Levosimendan; Oxidative stress; Reactive oxygen species; Rat testes; SOD; GSH-Px; MDA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800005

Determination of serum aluminum, platelet aggregation and lipid peroxidation in hemodialyzed patients
Provedor de dados: 56
Autores: Neiva,T.J.C.; Benedetti,A.L.; Tanaka,S.M.C.N.; Santos,J.I.; D'Amico,E.A.
Aluminum (Al3+) overload is frequently associated with lipid peroxidation and neurological disorders. Aluminum accumulation is also reported to be related to renal impairment, anemia and other clinical complications in hemodialysis patients. The aim of the present study was to determine the degree of lipid peroxidation, platelet aggregation and serum aluminum in patients receiving regular hemodialytic treatment. The level of plasma lipid peroxidation was evaluated on the basis of thiobarbituric acid reactive substances (TBARS). Mean platelet peroxidation in patients undergoing hemodialysis was significantly higher than in normal controls (2.7 ± 0.03 vs 1.8 ± 0.06 nmol/l, P&lt;0.05). Platelet aggregation and serum aluminum levels were determined by a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aluminum; Peroxidation; Hemodialysis; Platelets.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300009

Acute lead-induced vasoconstriction in the vascular beds of isolated perfused rat tails is endothelium-dependent
Provedor de dados: 56
Autores: Silveira,E.A.; Lizardo,J.H.F.; Souza,L.P.; Stefanon,I.; Vassallo,D.V.
Chronic lead exposure induces hypertension in humans and animals, affecting endothelial function. However, studies concerning acute cardiovascular effects are lacking. We investigated the effects of acute administration of a high concentration of lead acetate (100 µΜ) on the pressor response to phenylephrine (PHE) in the tail vascular bed of male Wistar rats. Animals were anesthetized with sodium pentobarbital and heparinized. The tail artery was dissected and cannulated for drug infusion and mean perfusion pressure measurements. Endothelium and vascular smooth muscle relaxation were tested with acetylcholine (5 µg/100 µL) and sodium nitroprusside (0.1 µg/100 µL), respectively, in arteries precontracted with 0.1 µM PHE. Concentration-response curves to PHE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lead acetate; Vascular reactivity; Endothelium; Hypertension.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500011

Retinal protective effects of topically administered agmatine on ischemic ocular injury caused by transient occlusion of the ophthalmic artery
Provedor de dados: 56
Autores: Hong,S.; Hara,H.; Shimazawa,M.; Hyakkoku,K.; Kim,C.Y.; Seong,G.J.
Agmatine, an endogenous polyamine and putative neuromodulator, is known to have neuroprotective effects on various neurons in the central nervous system. We determined whether or not topically administered agmatine could reduce ischemic retinal injury. Transient ocular ischemia was achieved by intraluminal occlusion of the middle cerebral artery of ddY mice (30-35 g) for 2 h, which is known to also induce occlusion of the ophthalmic artery. In the agmatine group (N = 6), a 1.0 mM agmatine-containing ophthalmic solution was administered four times daily for 2 weeks before occlusion. In the control group (N = 6), a 0.1% hyaluronic acid ophthalmic solution was instilled at the same times. At 22 h after reperfusion, the eyeballs were enucleated and the retinal...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Agmatine; Apoptosis; Ischemia; Neuroprotection; Retina.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300005

LXW7 ameliorates focal cerebral ischemia injury and attenuates inflammatory responses in activated microglia in rats
Provedor de dados: 56
Autores: Fang,T.; Zhou,D.; Lu,L.; Tong,X.; Wu,J.; Yi,L.
Inflammation plays a pivotal role in ischemic stroke, when activated microglia release excessive pro-inflammatory mediators. The inhibition of integrin αvβ3 improves outcomes in rat focal cerebral ischemia models. However, the mechanisms by which microglia are neuroprotective remain unclear. This study evaluated whether post-ischemic treatment with another integrin αvβ3 inhibitor, the cyclic arginine-glycine-aspartic acid (RGD) peptide-cGRGDdvc (LXW7), alleviates cerebral ischemic injury. The anti-inflammatory effect of LXW7 in activated microglia within rat focal cerebral ischemia models was examined. A total of 108 Sprague-Dawley rats (250–280 g) were subjected to middle cerebral artery occlusion (MCAO). After 2 h, the rats were given an intravenous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Activated microglia; Ischemic stroke; Integrin αvβ3; TNF-α.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900602

Protective role of antioxidant vitamin E and catechin on idarubicin-induced cardiotoxicity in rats
Provedor de dados: 56
Autores: Kalender,S.; Kalender,Y.; Ates,A.; Yel,M.; Olcay,E.; Candan,S.
Idarubicin is an anthracycline antibiotic extensively used in acute leukemia. In the present study we investigated whether vitamin E and catechin can reduce the toxic effects of idarubicin. Vitamin E (200 IU kg-1 week-1), catechin (200 mg kg-1 week-1), idarubicin (5 mg kg-1 week-1), idarubicin + vitamin E (200 IU kg-1 week-1), and idarubicin + catechin (200 mg kg-1 week-1) combinations were given to male Sprague-Dawley rats weighing 210 to 230 g (N = 6/group). Idarubicin-treated animals exhibited a decrease in body and heart weight, a decrease in myocardial contractility, and changes in ECG parameters (P&lt;0.01). Catechin + idarubicin- and vitamin E + idarubicin-treated groups exhibited similar alterations, but changes were attenuated in comparison to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Idarubicin; Catechin; Vitamin E; Cardiotoxicity; Ultrastructure.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100017

Humoral and cellular immune responses to Blomia tropicalis and concanavalin A-binding fractions in atopic patients
Provedor de dados: 56
Autores: Alves,R.; Silva,D.A.O.; Fernandes,J.F.C.; Almeida,K.C.; Ynoue,L.H.; Bernardes,C.T.V.; Moreira,P.F.S.; Gennari-Cardoso,M.L.; Sung,S.J.; Taketomi,E.A.
Blomia tropicalis, Dermatophagoides pteronyssinus and D. farinae are prevalent house dust mites. Concanavalin A-binding components derived from B. tropicalis (Bt-ConA extract) are highly immunogenic in allergic diseases. The aim of the present study was to evaluate the humoral and cellular immune responses to B. tropicalis in mite-sensitized patients. A total of 137 patients with allergic rhinitis with/without asthma and 109 non-atopic subjects were selected and analyzed by the skin prick test, and for total serum IgE and specific IgE levels to both Bt-total and Bt-ConA extracts, their proliferative response and cytokine (IFN-γ and IL-5) production by peripheral blood mononuclear cells (PBMC) stimulated with both extracts. Skin prick test showed that 70%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blomia tropicalis; Concanavalin A; Cellular proliferation; IFN-γ; IL-5; Immunoglobulin E.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900006

Epoetin alfa resistance in hemodialysis patients with chronic kidney disease: a longitudinal study
Provedor de dados: 56
Autores: Santos,E.J.F.; Hortegal,E.V.; Serra,H.O.; Lages,J.S.; Salgado-Filho,N.; dos Santos,A.M.
Anemia is an inevitable complication of hemodialysis, and the primary cause is erythropoietin deficiency. After diagnosis, treatment begins with an erythropoiesis-stimulating agent (ESA). However, some patients remain anemic even after receiving this medication. This study aimed to investigate the factors associated with resistance to recombinant human erythropoietin therapy with epoetin alfa (αEPO). We performed a prospective, longitudinal study of hemodialysis patients receiving treatment with αEPO at our reference hospital from July 2015 to June 2016. Clinical data was collected, and the response to αEPO treatment was evaluated using the erythropoietin resistance index (ERI). The ERI was defined as the weekly weight-adjusted αEPO dose (U/kg per...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epoetin alfa; Drug resistance; Renal insufficiency; Chronic disease; Renal dialysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700603

Changes in the hormone and lipid profile of obese adolescent Saudi females with acne vulgaris
Provedor de dados: 56
Autores: Abulnaja,K.O.
Acne vulgaris is a multifactorial disease affecting a majority of the adolescent population. The objective of this study was to test for a correlation between fasting serum lipid profiles and levels of testosterone, insulin, leptin, and interleukin 1-β (IL-1β) and the incidence of severe acne vulgaris in obese adolescent females. Four groups of adolescent females were studied: obese with acne, obese without acne, non-obese with acne, and non-obese without acne. Obese females with acne, compared to obese females without acne and non-obese subjects, had significantly higher serum triglycerides, low-density lipoprotein cholesterol and apolipoprotein-B (apo-B) (mean ± SD: 197 ± 13.7 vs 171 ± 11.5, 128 ± 8.3 vs 116 ± 7.7, 96 ± 13.7 vs 85 ± 10.3 mg/dL,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acne vulgaris; Obesity; Testosterone; Adolescent females; Serum IL-1β.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600005

MicroRNA-340-5p modulates cisplatin resistance by targeting LPAATβ in osteosarcoma
Provedor de dados: 56
Autores: Song,L.; Duan,P.; Gan,Y.; Li,P.; Zhao,C.; Xu,J.; Zhang,Z.; Zhou,Q.
MicroRNAs (miRNAs) play an important role in drug resistance and modulate the efficiency of chemotherapy. A recent study indicated that miR-340 functions as a tumor suppressor in various types of cancer. However, the role of miR-340 in chemotherapy has not been reported yet. In this study, we found that miR-340 enhanced cisplatin (CDDP)-induced cell death. Induction of miR-340-5p expression decreased the IC50 of CDDP and increased the apoptosis of CDDP-resistant MG-63 and Saos-2 cells. Moreover, miR-340-5p decreased the accumulation of MRP1 and MDR1. We further explored the mechanism underlying the promoting effects of miR-340-5p on CDDP-induced cell death. We identified a potential target of miR-340 in the 3′ untranslated region of lysophosphatidic acid...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Sensitivity to cisplatin; LPAATβ; MiR-340-5p; Osteosarcoma.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500606

Helicobacter pylori adhesion to gastric epithelial cells is mediated by glycan receptors
Provedor de dados: 56
Autores: Magalhães,A.; Reis,C.A.
Helicobacter pylori adhesion to gastric epithelial cells constitutes a key step in the establishment of a successful infection of the gastric mucosa. The high representation of outer membrane proteins in the bacterial genome suggests the relevance of those proteins in the establishment of profitable interactions with the host gastric cells. Gastric epithelial cells are protected by a mucous layer gel, mainly consisting of the MUC5AC and MUC6 mucins. In addition to this protective role, mucins harbor glycan-rich domains that constitute preferential binding sites of many pathogens. In this article we review the main players in the process of H. pylori adhesion to gastric epithelial cells, which contribute decisively to the high prevalence and chronicity of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Bacterial adhesins; Lewis antigens; Glycan receptors; Chronic gastritis; Gastric carcinogenesi.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700002

Protection against neurotoxicity by an autophagic mechanism
Provedor de dados: 56
Autores: Liu,Kangyong; Huang,Jiankang; Chen,Rongfu; Zhang,Ting; Shen,Liwei; Yang,Jiajun; Sun,Xiaojiang.
The objective of the present study was to investigate the effects of 3-n-butylphthalide (NBP) on a 1-methyl-4-phenylpyridinium (MPP+)-induced cellular model of Parkinson’s disease (PD) and to illustrate the potential mechanism of autophagy in this process. For this purpose, rat PC12 pheochromocytoma cells were treated with MPP+ (1 mM) for 24 h following pretreatment with NBP (0.1 mM). Cell metabolic viability was determined by the MTT assay and cell ultrastructure was examined by transmission electron microscopy. The intracellular distribution and expression of α-synuclein and microtubule-associated protein light chain 3 (LC3) were detected by immunocytochemistry and Western blotting. Our results demonstrated that: 1) NBP prevented MPP+-induced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 3-N-butylphthalide; Parkinson’s disease; Α-synuclein; PC12 cells; Autophagy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500004

Influence of storage time on DNA of Chlamydia trachomatis, Ureaplasma urealyticum, and Neisseria gonorrhoeae for accurate detection by quantitative real-time polymerase chain reaction
Provedor de dados: 56
Autores: Lu,Y.; Rong,C.Z.; Zhao,J.Y.; Lao,X.J.; Xie,L.; Li,S.; Qin,X.
The shipment and storage conditions of clinical samples pose a major challenge to the detection accuracy of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Ureaplasma urealyticum (UU) when using quantitative real-time polymerase chain reaction (qRT-PCR). The aim of the present study was to explore the influence of storage time at 4°C on the DNA of these pathogens and its effect on their detection by qRT-PCR. CT, NG, and UU positive genital swabs from 70 patients were collected, and DNA of all samples were extracted and divided into eight aliquots. One aliquot was immediately analyzed with qRT-PCR to assess the initial pathogen load, whereas the remaining samples were stored at 4°C and analyzed after 1, 2, 3, 7, 14, 21, and 28 days. No...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Storage time; Chlamydia trachomatis; Ureaplasma urealyticum; Neisseria gonorrhoeae; Stability; QRT-PCR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000701

Effect of 5-HT1B receptor agonists injected into the prefrontal cortex on maternal aggression in rats
Provedor de dados: 56
Autores: Veiga,C.P.; Miczek,K.A.; Lucion,A.B.; Almeida,R.M.M. de.
Serotonin (5-HT1B) receptors play an essential role in the inhibition of aggressive behavior in rodents. CP-94,253, a 5-HT1B receptor agonist, can reduce aggression in male mice when administered directly into the ventro-orbitofrontal (VO) prefrontal cortex (PFC). The objective of the current study was to assess the effects of two selective 5-HT1B receptor agonists (CP-94,253 and CP-93,129), microinjected into the VO PFC, on maternal aggressive behavior after social instigation in rats. CP-94,253 (0.56 µg/0.2 µL, N = 8, and 1.0 µg/0.2 µL, N = 8) or CP-93,129 (1.0 µg/0.2 µL, N = 9) was microinjected into the VO PFC of Wistar rats on the 9th day postpartum and 15 min thereafter the aggressive behavior by the resident female against a male intruder was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Prefrontal cortex; 5-HT receptors; Serotonin; Maternal behavior; Aggression.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600012

Group cognitive behavior therapy for bipolar disorder can improve the quality of life
Provedor de dados: 56
Autores: Costa,R.T.; Cheniaux,E.; Rangé,B.P.; Versiani,M.; Nardi,A.E.
Bipolar disorder (BD) can have an impact on psychosocial functioning and quality of life (QoL). Several studies have shown that structured psychotherapy in conjunction with pharmacotherapy may modify the course of some disorders; however, few studies have investigated the results of group cognitive behavior therapy (G-CBT) for BD. Our objective was to evaluate the effectiveness of 14 sessions of G-CBT for BD patients, comparing this intervention plus pharmacotherapy to treatment as usual (TAU; only pharmacotherapy). Forty-one patients with BD I and II participated in this study and were randomly allocated to each group (G-CBT: N = 27; TAU: N = 14). Thirty-seven participants completed the treatment (women: N = 66.67%; mean age = 41.5 years). QoL and mood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Group therapy; Psychotherapy; Bipolar disorder; Mania; Depression.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900011

Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil
Provedor de dados: 56
Autores: Andrade,M.K.N.; Machado,S.M.A.; Leite,M.L.; Saad,M.H.F.
Assuming that the IS6110-restriction fragment length polymorphism (RFLP) changes at a constant rate of 3.2 years, this methodology was applied to demonstrate, for the first time, variant patterns of Mycobacterium tuberculosis (MTB) in multiple isolates obtained at short time intervals from sputum and blood of an HIV+ patient with multiple admissions to the Emergency Room and to the multidrug-resistant tuberculosis (MDR-TB) Reference Center of a secondary-care hospital in Rio de Janeiro, Brazil. In sputum, the IS6110-RFLP appeared in isolates with two variant patterns with 10 and 13 IS6110 copies. However, blood presented only the pattern corresponding to 10 copies, suggesting compartmentalization. With regard to the exact match of 10 of 13 bands, this may...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tuberculosis; Mycobacterium tuberculosis; TB-MDR; TB-RFLP; Gene mutation; Fingerprint.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500006

Purine nucleotides reduce superoxide production by nitric oxide synthase in a murine sepsis model
Provedor de dados: 56
Autores: Barbeiro,H.V.; Barbeiro,D.F.; Debbas,V.; Souza,H.P.; Laurindo,F.R.; Velasco,I.T.; Soriano,F.G.
Sepsis involves a systemic inflammatory response of multiple endogenous mediators, resulting in many of the injurious and sometimes fatal physiological symptoms of the disease. This systemic activation leads to a compromised vascular response and endothelial dysfunction. Purine nucleotides interact with purinoceptors and initiate a variety of physiological processes that play an important role in maintaining cardiovascular function. The purpose of the present study was to investigate the effects of ATP on vascular function in a lipopolysaccharide (LPS) model of sepsis. LPS induced a significant increase in aortic superoxide production 16 h after injection. Addition of ATP to the organ bath incubation solution reduced superoxide production by the aortas of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ATP; Endotoxin; Endothelium; Superoxide; Nitric oxide.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100009

Fractures of the proximal femur: correlation with vitamin D receptor gene polymorphism
Provedor de dados: 56
Autores: Ramalho,A.C.; Lazaretti-Castro,M.; Hauache,O.; Kasamatsu,T.; Brandão,C.; Reis,A.F.; Takata,E.; Cafalli,F.; Tavares,F.; Gimeno,S.G.A.; Vieira,J.G.H.
Fractures are the feared consequences of osteoporosis and fractures of the proximal femur (FPF) are those that involve the highest morbidity and mortality. Thus far, evaluation of bone mineral density (BMD) is the best way to determine the risk of fracture. Genetic inheritance, in turn, is one of the major determinants of BMD. A correlation between different genotypes of the vitamin D receptor (VDR) and BMD has been recently reported. On this basis, we decided to determine the importance of the determination of VDR genotype in the presence of an osteoporotic FPF in a Brazilian population. We studied three groups: group I consisted of 73 elderly subjects older than 65 years (78.5 ± 7.2 years) hospitalized for nonpathological FPF; group II consisted of 50...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoporosis; Fracture of proximal femur (FPF); Vitamin D receptor polymorphism.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700006

Effects of eugenol on resting tension of rat atria
Provedor de dados: 56
Autores: Olivoto,R.R.; Damiani,C.E.N.; Kassouf Silva,I.; Lofrano-Alves,M.S.; Oliveira,M.A.; Fogaça,R.T.H.
In cardiac and skeletal muscle, eugenol (μM range) blocks excitation-contraction coupling. In skeletal muscle, however, larger doses of eugenol (mM range) induce calcium release from the sarcoplasmic reticulum. The effects of eugenol are therefore dependent on its concentration. In this study, we evaluated the effects of eugenol on the contractility of isolated, quiescent atrial trabeculae from male Wistar rats (250-300 g; n=131) and measured atrial ATP content. Eugenol (1, 3, 5, 7, and 10 mM) increased resting tension in a dose-dependent manner. Ryanodine [100 µM; a specific ryanodine receptor (RyR) blocker] and procaine (30 mM; a nonspecific RyR blocker) did not block the increased resting tension induced by eugenol regardless of whether extracellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eugenol; Atrial trabeculae; Resting tension; Ryanodine; Rat; ATP.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400328

Prognosis of colorectal cancer with liver metastasis: value of a prognostic index
Provedor de dados: 56
Autores: Wang,Y.; Liu,Y.F.; Cheng,Y.; Yi,D.H.; Li,P.; Song,W.Q.; Fu,D.Z.; Wang,X.
The objective of the present study was to explore the factors related to the prognosis of colorectal cancer (CRC) and to establish a prognostic model for the selection of patients who might benefit from hepatic resection for metastatic CRC. A total of 293 patients undergoing liver resection for metastatic CRC (172 males and 80 females ranging in age from 26 to 80 years) were selected and clinical, pathological and outcome data were examined in this retrospective study. The prognostic index (PI) of the patients was calculated on the basis of results of multivariate analysis. Patients were stratified into different groups, with survival curves projected according to PI. The 1-, 3-, and 5-year overall survival rates were 58.3, 26.4, and 11.3%, respectively....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colorectal cancer; Liver metastasis; Prognosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100015

Activation of locus coeruleus heme oxygenase-carbon monoxide pathway promoted an anxiolytic-like effect in rats
Provedor de dados: 56
Autores: Carvalho-Costa,P.G.; Branco,L.G.S.; Leite-Panissi,C.R.A.
The heme oxygenase-carbon monoxide pathway has been shown to play an important role in many physiological processes and is capable of altering nociception modulation in the nervous system by stimulating soluble guanylate cyclase (sGC). In the central nervous system, the locus coeruleus (LC) is known to be a region that expresses the heme oxygenase enzyme (HO), which catalyzes the metabolism of heme to carbon monoxide (CO). Additionally, several lines of evidence have suggested that the LC can be involved in the modulation of emotional states such as fear and anxiety. The purpose of this investigation was to evaluate the activation of the heme oxygenase-carbon monoxide pathway in the LC in the modulation of anxiety by using the elevated plus maze test (EPM)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heme oxygenase Carbon monoxide Locus coeruleus Anxiety Elevated plus maze; Light/dark box.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500601

Centromere domain organization and histone modifications
Provedor de dados: 56
Autores: Bjerling,P.; Ekwall,K.
Centromere function requires the proper coordination of several subfunctions, such as kinetochore assembly, sister chromatid cohesion, binding of kinetochore microtubules, orientation of sister kinetochores to opposite spindle poles, and their movement towards the spindle poles. Centromere structure appears to be organized in different, separable domains in order to accomplish these functions. Despite the conserved nature of centromere functions, the molecular genetic definition of the DNA sequences that form a centromere in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, in the fruit fly Drosophila melanogaster, and in humans has revealed little conservation at the level of centromere DNA sequences. Also at the protein level few...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Centromere; Kinetochore; Histone acetylation; Electron microscopy.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500001

Esculetin, a coumarin derivative, exerts in vitro and in vivo antiproliferative activity against hepatocellular carcinoma by initiating a mitochondrial-dependent apoptosis pathway
Provedor de dados: 56
Autores: Wang,J.; Lu,M.L.; Dai,H.L.; Zhang,S.P.; Wang,H.X.; Wei,N.
This study investigated the in vitro and in vivo antiproliferative activity of esculetin against hepatocellular carcinoma, and clarified its potential molecular mechanisms. Cell viability was determined by the MTT (tetrazolium) colorimetric assay. In vivo antitumor activity of esculetin was evaluated in a hepatocellular carcinoma mouse model. Seventy-five C57BL/6J mice were implanted with Hepa1-6 cells and randomized into five groups (n=15 each) given daily intraperitoneal injections of vehicle (physiological saline), esculetin (200, 400, or 700 mg·kg-1·day-1), or 5-Fu (200 mg·kg-1·day-1) for 15 days. Esculetin significantly decreased tumor growth in mice bearing Hepa1-6 cells. Tumor weight was decreased by 20.33, 40.37, and 55.42% with increasing doses of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esculetin; Apoptosis; Hepatocellular carcinoma; Mitochondrial-dependent pathway; Antitumor activity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300245

Aging and immunoglobulin isotype patterns in oral tolerance
Provedor de dados: 56
Autores: Faria,A.M.C.; Ficker,S.M.; Speziali,E.; Menezes,J.S.; Stransky,B.; Verdolin,B.A.; Lahmann,W.M.; Rodrigues,V.S.; Vaz,N.M.
In the present review we address oral tolerance as an important biological phenomenon and discuss how it is affected by aging. Other factors such as frequency of feeding and previous digestion of the antigen also seem to influence the establishment of oral tolerance. We also analyze immunoglobulin isotypes of specific antibodies formed by tolerant and immunized animals of different ages submitted to different conditions of oral antigen administration. Isotypic patterns were studied as a parameter for assessing the pathways of B and T cell interactions leading to antibody production
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral tolerance; Aging; Isotypes; Gut; Mucosa.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100005

Voluntary wheel running: patterns and physiological effects in mice
Provedor de dados: 56
Autores: Manzanares,G.; Brito-da-Silva,G.; Gandra,P.G.
Exercise can prevent and improve the pathophysiology of diseases and promote healthy aging. Thus, understanding the mechanisms that regulate the beneficial effects of exercise may lead to the development of new strategies to enhance quality of life and to counteract chronic diseases. Voluntary wheel running is an interesting model to study the effects of exercise in mice. Compared to forced treadmill exercise, voluntary wheel running presents several advantages such as: 1) running pattern is similar to natural running behavior of mice; 2) it is performed under non-stressed conditions, according to the rhythmicity of the animal; 3) it does not require direct interference from the researcher, and can be easily applied in long-term studies. Mice run...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Voluntary wheel running; Mouse; Exercise; Skeletal muscle; Endurance adaptation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100301

Effects of exercise training on autonomic and myocardial dysfunction in streptozotocin-diabetic rats
Provedor de dados: 56
Autores: De Angelis,K.L.D.; Oliveira,A.R.; Dall'Ago,P.; Peixoto,L.R.A.; Gadonski,G.; Lacchini,S.; Fernandes,T.G.; Irigoyen,M.C.
Several investigators have demonstrated that diabetes is associated with autonomic and myocardial dysfunction. Exercise training is an efficient non-pharmacological treatment for cardiac and metabolic diseases. The aim of the present study was to investigate the effects of exercise training on hemodynamic and autonomic diabetic dysfunction. After 1 week of diabetes induction (streptozotocin, 50 mg/kg, iv), male Wistar rats (222 ± 5 g, N = 18) were submitted to exercise training for 10 weeks on a treadmill. Arterial pressure signals were obtained and processed with a data acquisition system. Autonomic function and intrinsic heart rate were studied by injecting methylatropine and propranolol. Left ventricular function was assessed in hearts perfused in vitro...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental diabetes; Exercise training; Arterial pressure; Autonomic control; Myocardial contractility.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600004

Frequency of primary glomerular disease in northeastern China
Provedor de dados: 56
Autores: Wu,Yi-Qi; Wang,Zheng; Xu,Hua-Feng; Jin,Xiao-Ming; Zhou,Hai-Zhou.
Most frequently reported Chinese renal biopsy data have originated from southeastern China. The present study analyzed the renal biopsy data from northeastern China. The records of 1550 consecutive native patients who were diagnosed with primary glomerular diseases (PGD) after renal biopsy at our hospital during 2005-2009 were used. These patients were divided into four age groups for stratified analysis: &lt;15, 15-44, 45-59, and ≥60 years old. Among PGD, minimal change disease (MCD) was the most common histologically diagnosed disease (30.7%), followed by IgA nephropathy (IgAN), mesangial proliferative glomerulonephritis (MsPGN), membranous nephropathy (MN), membranoproliferative glomerulonephritis (MPGN), focal segmental glomerulosclerosis (FSGS), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Primary glomerular disease; Renal biopsies; IgA nephropathy; China.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800011

Adenovirus-mediated siRNA targeting TNF-α and overexpression of bone morphogenetic protein-2 promotes early osteoblast differentiation on a cell model of Ti particle-induced inflammatory response in vitro
Provedor de dados: 56
Autores: Guo,H.H.; Yu,C.C.; Sun,S.X.; Ma,X.J.; Yang,X.C.; Sun,K.N.; Jin,Q.H.
Wear particles are phagocytosed by macrophages and other inflammatory cells, resulting in cellular activation and release of proinflammatory factors, which cause periprosthetic osteolysis and subsequent aseptic loosening, the most common causes of total joint arthroplasty failure. During this pathological process, tumor necrosis factor-alpha (TNF-α) plays an important role in wear-particle-induced osteolysis. In this study, recombination adenovirus (Ad) vectors carrying both target genes [TNF-α small interfering RNA (TNF-α-siRNA) and bone morphogenetic protein 2 (BMP-2)] were synthesized and transfected into RAW264.7 macrophages and pro-osteoblastic MC3T3-E1 cells, respectively. The target gene BMP-2, expressed on pro-osteoblastic MC3T3-E1 cells and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TNF-α; BMP-2; Small interfering RNA; Titanium particles; Osteolysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000831

Relative contribution of expectancy and immediate arousal to the facilitatory effect of an auditory accessory stimulus
Provedor de dados: 56
Autores: Del-Fava,F.; Ribeiro-do-Valle,L.E.
An auditory stimulus speeds up a digital response to a subsequent visual stimulus. This facilitatory effect has been related to the expectancy and the immediate arousal that would be caused by the accessory stimulus. The present study examined the relative contribution of these two influences. In a first and a third experiment a simple reaction time task was used. In a second and fourth experiment a go/no-go reaction time task was used. In each of these experiments, the accessory stimulus preceded the target stimulus by 200 ms for one group of male and female volunteers (G Fix). For another group of similar volunteers (G Var) the accessory stimulus preceded the target stimulus by 200 ms in 25% of the trials, by 1000 ms in 25% of the trials and was not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Priming; Arousal; Alertness; Expectancy; Attention.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800006

Prolonged acceptance of skin grafts induced by B cells places regulatory T cells on the histopathology scene
Provedor de dados: 56
Autores: Langier,S.; Galvani,R.G.; Alves,A.P.G.; Fidelis,R.; Nunes,P.H.C.; Silva,M.H.; Castilho,L.R.; Monteiro,J.P.; Bonomo,A.
The participation of regulatory T (Treg) cells in B cell-induced T cell tolerance has been claimed in different models. In skin grafts, naive B cells were shown to induce graft tolerance. However, neither the contribution of Treg cells to B cell-induced skin tolerance nor their contribution to the histopathological diagnosis of graft acceptance has been addressed. Here, using male C57BL/6 naive B cells to tolerize female animals, we show that skin graft tolerance is dependent on CD25+ Treg cell activity and independent of B cell-derived IL-10. In fact, B cells from IL-10-deficient mice were able to induce skin graft tolerance while Treg depletion of the host inhibited 100% graft survival. We questioned how Treg cell-mediated tolerance would impact on...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Skin graft; Tolerance; B cells; Treg; Histopathology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000009

Immunological properties of gene vaccines delivered by different routes
Provedor de dados: 56
Autores: Oliveira,S.C.; Rosinha,G.M.S.; de-Brito,C.F.A.; Fonseca,C.T.; Afonso,R.R.; Costa,M.C.M.S.; Goes,A.M.; Rech,E.L.; Azevedo,V.
Gene vaccines represent a new and promising approach to control infectious diseases, inducing a protective immune response in the appropriate host. Several routes and methods of genetic immunization have been shown to induce antibody production as well as T helper (Th) cell and cytotoxic T lymphocyte activation. However, few studies have compared the nature of the immune responses generated by different gene vaccination delivery systems. In the present study we reviewed some aspects of immunity induced by gene immunization and compared the immune responses produced by intramuscular (im) DNA injection to gene gun-mediated DNA transfer into the skin of BALB/c mice. Using a reporter gene coding for ß-galactosidase, we have demonstrated that im injection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene vaccination; Naked DNA; Gene gun; Cytokines; Immune response.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200009

Apolipoprotein B and angiotensin-converting enzyme polymorphisms and aerobic interval training: randomized controlled trial in coronary artery disease patients
Provedor de dados: 56
Autores: Tamburus,N.Y.; Verlengia,R.; Kunz,V.C.; César,M.C.; Silva,E.
Physical training has been strongly recommended as a non-pharmacological treatment for coronary artery disease (CAD). Genetic polymorphisms have been studied to understand the biological variability in response to exercise among individuals. This study aimed to verify the possible influence of apolipoprotein B (ApoB: rs1042031 and rs693) and angiotensin-converting enzyme (ACE-ID: rs1799752) genotypes on the lipid profile and functional aerobic capacity, respectively, after an aerobic interval training (AIT) program in patients with CAD and/or cardiovascular risk factors. Sixty-six men were randomized and assigned to trained group (n=32) or control group (n=34). Cardiopulmonary exercise test was performed to determine the ventilatory anaerobic threshold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physical training; Lipids; Apolipoprotein B polymorphism; Angiotensin-converting enzyme polymorphism; Aerobic capacity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800601

Human umbilical cord blood cells in infarcted rats
Provedor de dados: 56
Autores: Pinho-Ribeiro,V.; Maia,A.C.V.; Werneck-de-Castro,J.P S.; Oliveira,P.F.; Goldenberg,R.C.S.; Campos de Carvalho,A.C.
Therapy with bone marrow-derived cells has been used in ischemic patients with reported success. The aim of this study was to determine the therapeutic efficacy of fresh and frozen human umbilical cord blood cells (hUCB) in Wistar rats submitted to permanent occlusion of the left coronary artery. Three hours after myocardial infarction, 2 x 10(7) hUCB cells or vehicle were administered by intramyocardial injection. The animals were divided into five groups: control (N = 10), sham operated (N = 10), infarcted that received vehicle (N = 9), infarcted treated with cryopreserved hUCB (N = 7), and infarcted treated with fresh hUCB (N = 5). Cardiac function was evaluated by electrocardiogram (ECG) and echocardiogram (ECHO) before cell therapy, and by ECG, ECHO,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell therapy; Fresh and frozen human umbilical cord blood; Acute myocardial infarction; Electrocardiogram; Echocardiogram.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300011

Normal skin and hypertrophic scar fibroblasts differentially regulate collagen and fibronectin expression as well as mitochondrial membrane potential in response to basic fibroblast growth factor
Provedor de dados: 56
Autores: Song,Rui; Bian,Hui-Ning; Lai,Wen; Chen,Hua-De; Zhao,Ke-Seng.
Basic fibroblast growth factor (bFGF) regulates skin wound healing; however, the underlying mechanism remains to be defined. In the present study, we determined the effects of bFGF on the regulation of cell growth as well as collagen and fibronectin expression in fibroblasts from normal human skin and from hypertrophic scars. We then explored the involvement of mitochondria in mediating bFGF-inducedeffects on the fibroblasts. We isolated and cultivated normal and hypertrophic scar fibroblasts from tissue biopsies of patients who underwent plastic surgery for repairing hypertrophic scars. The fibroblasts were then treated with different concentrations of bFGF (ranging from 0.1 to 1000 ng/mL). The growth of hypertrophic scar fibroblasts became slower with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BFGF; Hypertrophic scar; Fibroblast; Extracellular matrix; Mitochondria; Gene expression.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500004

A priori estimation of accuracy and of the number of wells to be employed in limiting dilution assays
Provedor de dados: 56
Autores: Chaui-Berlinck,J.G.; Barbuto,J.A.M.
The use of limiting dilution assay (LDA) for assessing the frequency of responders in a cell population is a method extensively used by immunologists. A series of studies addressing the statistical method of choice in an LDA have been published. However, none of these studies has addressed the point of how many wells should be employed in a given assay. The objective of this study was to demonstrate how a researcher can predict the number of wells that should be employed in order to obtain results with a given accuracy, and, therefore, to help in choosing a better experimental design to fulfill one's expectations. We present the rationale underlying the expected relative error computation based on simple binomial distributions. A series of simulated in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Limiting dilution assay: accuracy; Wells; Number of; Relative error.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800010

Thalamic metabolic abnormalities in patients with Huntington's disease measured by magnetic resonance spectroscopy
Provedor de dados: 56
Autores: Casseb,R.F.; D'Abreu,A.; Ruocco,H.H.; Lopes-Cendes,I.; Cendes,F.; Castellano,G.
Huntington's disease (HD) is a neurologic disorder that is not completely understood; its fundamental physiological mechanisms and chemical effects remain somewhat unclear. Among these uncertainties, we can highlight information about the concentrations of brain metabolites, which have been widely discussed. Concentration differences in affected, compared to healthy, individuals could lead to the development of useful tools for evaluating the progression of disease, or to the advance of investigations of different/alternative treatments. The aim of this study was to compare the thalamic concentration of metabolites in HD patients and healthy individuals using magnetic resonance spectroscopy. We used a 2.0-Tesla magnetic field, repetition time of 1500 ms,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnetic resonance spectroscopy; Huntington's disease; Quantification; Thalamus; LCModel.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800722

Erratum for: miR-26a-5p protects against myocardial ischemia/reperfusion injury by regulating the PTEN/PI3K/AKT signaling pathway
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/other
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300901

Increased sympathetic activity in normotensive offspring of malignant hypertensive parents compared to offspring of normotensive parents
Provedor de dados: 56
Autores: Lopes,H.F.; Consolim-Colombo,F.M.; Barreto-Filho,J.A.S.; Riccio,G.M.G.; Negrão,C.E.; Krieger,E.M.
Malignant hypertension seems to be the consequence of very high blood pressure. Furthermore, an increase in sympathetic and renin-angiotensin system activity is considered to be the main mechanisms producing malignant hypertension. In the present study, 10 offspring of malignant hypertensive (OMH) parents (age 28 ± 5 years, 7 males, 3 females, 2 white and 8 non-white) and 10 offspring of normotensive (ONT) parents (age 28 ± 6 years, 2 males, 8 females, 3 white and 7 non-white) were evaluated. The OMH group had significantly higher (P &lt; 0.05) casual blood pressure (125 ± 10/81 ± 5 mmHg) compared with ONT (99 ± 13/67 ± 5 mmHg). The increase in blood pressure was greater in OMH (Δ SBP = 17 ± 2 vs Δ SBP = 9 ± 1 mmHg in ONT) during cold pressor testing, but...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Malignant hypertension; Offspring of malignant hypertensive parents; Sympathetic activity; Microneurography.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000003

Immunologic patterns associated with cure in human American cutaneous leishmaniasis
Provedor de dados: 56
Autores: Coutinho,S.G.; Da-Cruz,A.M.; Bertho,A.L.; Santiago,M.A.; De-Luca,P.
Patients with American cutaneous leishmaniasis were studied before therapy (active lesion) and at the end of therapy (cured patients). Assays of lymphocyte proliferative responses of peripheral blood mononuclear cells induced in vitro by Leishmania braziliensis promastigote antigens (Lb) were performed. Antigen-stimulated cells were harvested for CD4 and CD8 phenotype analysis and the levels of gamma interferon (IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;) and interleukin 4 (IL-4) produced were also determined in the culture supernatants. Two different patterns of Lb-induced T cell responses were observed: a) predominance of responding CD4+ cells and mixed type 1 and type 2 cytokine production (IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; and IL-4) during...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human cutaneous leishmaniasis Leishmania braziliensis CD4+ and CD8+ cells IFN-&lt; FONT FACE=Symbol&gt; G&lt; /font&gt; IL-4.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100019

Low pH and calcium effects on net Na+ and K+ fluxes in two catfish species from the Amazon River (Corydoras: Callichthyidae)
Provedor de dados: 56
Autores: Matsuo,A.Y.; Val,A.L.
The present study analyzes Na+ and K+ disturbances caused by low pH in two catfish species from the Amazon River. Corydoras adolfoi inhabits ion-poor, black-stained, low pH (3.5-4.0) waters, while C. schwartzi is native to ion-rich waters at circumneutral pH. Fish were exposed to pH 3.5 Ca2+-free, and Ca2+-enriched (~500 µmol/l) water to determine the protective effects of calcium. Net Na+ and K+ fluxes were measured in the water collected from the fish experimental chambers. C. adolfoi was unable to control the Na+ efflux at low pH, exhibiting Na+ loss up to -594 ± 84 nmol g-1 h-1 during the first hour. After 3 and 6 h, net Na+ flux increased by 7- and 23-fold, respectively. In C. schwartzi, at pH 3.5, the initial high Na+ loss (-1,063 ± 73 nmol g-1 h-1)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Low pH; Calcium; Blackwater; Net Na+ and K+ flux; Amazon; Catfish.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300012

Dynamics of immunosuppression in hamsters with experimental visceral leishmaniasis
Provedor de dados: 56
Autores: Fazzani,C.; Guedes,P.A.; Senna,A.; Souza,E.B.; Goto,H.; Lindoso,J.A.L.
Immunosuppression has been reported to occur during active visceral leishmaniasis and some factors such as the cytokine profile may be involved in this process. In the mouse model of cutaneous leishmaniasis using Leishmania (Leishmania) major, the Th1 response is related to protection while the Th2 response is related to disease progression. However, in hamsters, which are considered to be an excellent model for the study of visceral leishmaniasis, this dichotomy is not observed. Using outbred 45- to 60-day-old (140 to 150 g) male hamsters infected intraperitoneally with 2 x 10(7) L. (L.) chagasi amastigotes, we evaluated the immune response of spleen cells and the production of cytokines. We used 3 to 7 hamsters per group evaluated. We detected a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunosuppression; Leishmania(Leishmania) chagasi; Hamster; Cytokine; T-cell proliferation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700009

No evidence for mutations in exons 1, 8 and 18 of the patched gene in sporadic skin lesions of Brazilian patients
Provedor de dados: 56
Autores: Granja,F.; Santarosa,P.L.; Leite,J.L.A.A.P.; Ward,L.S.
There is strong evidence that the patched (PTCH) gene is a gene for susceptibility to the nevoid basal cell carcinoma syndrome. PTCH has also been shown to mutate in both familial and sporadic basal cell carcinomas. However, mutations of the gene seem to be rare in squamous cell carcinomas. In order to characterize the role of the gene in the broader spectrum of sporadic skin malignant and pre-malignant lesions, we performed a polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis of genomic DNA extracted from 105 adult patients (46 females and 59 males). There were 66 patients with basal cell carcinomas, 30 with squamous cell carcinomas, 2 with malignant melanomas and 7 patients with precancerous lesions. Two tissue samples...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Skin; Cancer; PTCH; Mutation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400006

Effects of short-term administration of estradiol on reperfusion arrhythmias in rats of different ages
Provedor de dados: 56
Autores: Savergnini,S.Q.; Reis,A.M.; Santos,R.A.S.; Santos,P.E.B.; Ferreira,A.J.; Almeida,A.P.
Little is known about age-related differences in short-term effects of estradiol on ischemia-reperfusion (I/R) insults. The present study was designed to evaluate the effects of short-term treatment with estradiol on reperfusion arrhythmias in isolated hearts of 6-7-week-old and 12-14-month-old female rats. Wistar rats were sham-operated, ovariectomized and treated with vehicle or ovariectomized and treated with 17β-estradiol (E2; 5 µg·100 g-1·day-1) for 4 days. Hearts were perfused by the Langendorff technique. Reperfusion arrhythmias, i.e., ventricular tachycardia and/or ventricular fibrillation, were induced by 15 min of left coronary artery ligation and 30 min of reperfusion. The duration and incidence of I/R arrhythmias were significantly higher in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardioprotection; Estrogen; Ischemia/reperfusion; Langendorff technique; QT interval.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200020

Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
Provedor de dados: 56
Autores: Nunes,V.A.; Gozzo,A.J.; Juliano,M.A.; Cerqueira César,M.; Sampaio,M.U.; Sampaio,C.A.M.; Araújo,M.S.
Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Caspase activation; Apoptosis; Dietary selenium deficiency; Dietary vitamin E deficiency; Internucleosomal DNA fragmentation; Caspase substrate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800010

microRNA-3129 promotes cell proliferation in gastric cancer cell line SGC7901 via positive regulation of pRb
Provedor de dados: 56
Autores: Yang,Shaofeng; Sheng,Nan; Pan,Lili; Cao,Jing; Liu,Jiao; Ma,Ran.
Several microRNAs (miRNAs) have been reported as oncogenes or tumor suppressors in many cancers, including gastric cancer (GC). However, the role and molecular mechanism of miR-3129 in GC is largely unknown. We aimed to explore the function and the underlying molecular mechanism of miR-3129 in GC. Cancer tissues and corresponding adjacent tissues were collected from 50 patients with GC, and the expression of miR-3129 was detected by RT-qPCR. The expression of miR-3129 and pRb in human GC cell line SCG7091 was altered by transient transfection. Thereafter, MTT and flow cytometry assays were used to analyze cell viability and cell cycle. The expression of cyclin E, CDK2, CDK2 inhibitors (p16 and 21), and pRb were detected by RT-qPCR and western blot. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNA-3129; Gastric cancer; PRb; Proliferation; Cell cycle.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600612

Determination of anaerobic threshold in rats using the lactate minimum test
Provedor de dados: 56
Autores: Voltarelli,F.A.; Gobatto,C.A.; Mello,M.A.R. de.
The break point of the curve of blood lactate vs exercise load has been called anaerobic threshold (AT) and is considered to be an important indicator of endurance exercise capacity in human subjects. There are few studies of AT determination in animals. We describe a protocol for AT determination by the "lactate minimum test" in rats during swimming exercise. The test is based on the premise that during an incremental exercise test, and after a bout of maximal exercise, blood lactate decreases to a minimum and then increases again. This minimum value indicates the intensity of the AT. Adult male (90 days) Wistar rats adapted to swimming for 2 weeks were used. The initial state of lactic acidosis was obtained by making the animals jump into the water and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rat; Blood lactate; Lactate minimum test; Maximal lactate steady state.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100018

Evaluation of the effects of Uncaria rhynchophylla alkaloid extract on LPS-induced preeclampsia symptoms and inflammation in a pregnant rat model
Provedor de dados: 56
Autores: Wu,Liang-Zhi; Xiao,Xiao-Min.
Excessive pro-inflammatory cytokines result in adverse pregnancy outcomes, including preeclampsia-like phenotypes, and fetal growth restriction. Anti-inflammation might be an effective therapy. The aim of this research was to investigate whether Uncaria rhynchophylla alkaloid extract (URE), a highly safe anti-inflammation constituent of the herb, can inhibit inflammation and improve clinical characteristics of preeclampsia in a lipopolysaccharide (LPS)-induced preeclampsia rat model. The rat model was established by daily administration of LPS (1 μg/kg body weight per day) from gestational day (GD) 14 to 19. Different doses of URE (35, 70, and 140 mg/kg body weight per day) were administered from GD 14 to GD 19. The effects of URE on proteinuria, maternal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preeclampsia; Inflammation; Uncaria rhynchophylla; Alkaloids.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600602

The compulsive-like aspect of the head dipping emission in rats with chronic electrolytic lesion in the area of the median raphe nucleus
Provedor de dados: 56
Autores: Hoshino,K.; Uga,D.A.; de Paula,H.M.G.
Head dipping (HD) is a behavioral pattern considered to have a risk assessment or an exploratory role and is used as a complementary parameter to evaluate anxiety in experimental animals. Since rats with electrolytic lesion in the area of the median raphe nucleus displayed high frequencies of HD in a previous study, the present investigation was undertaken to confirm this observation and to determine its anxiety-related origin. HD episodes were counted in adult male Wistar rats (270-350 g) with electrolytic lesion (N = 11) and sham-lesioned controls (N = 12). When HD was measured for 60 min on an elevated open platform, lesioned rats emitted 13 times more HD than controls (264.7 ± 93.3 vs 20.3 ± 7.6 episodes), with the difference being statistically...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Head dipping; Repetitive behavior; Median raphe nucleus; Electrolytic lesion; Rat.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200012

Salt-induced epithelial-to-mesenchymal transition in Dahl salt-sensitive rats is dependent on elevated blood pressure
Provedor de dados: 56
Autores: Wang,Y.; Mu,J.J.; Liu,F.Q.; Ren,K.Y.; Xiao,H.Y.; Yang,Z.; Yuan,Z.Y.
Dietary salt intake has been linked to hypertension and cardiovascular disease. Accumulating evidence has indicated that salt-sensitive individuals on high salt intake are more likely to develop renal fibrosis. Epithelial-to-mesenchymal transition (EMT) participates in the development and progression of renal fibrosis in humans and animals. The objective of this study was to investigate the impact of a high-salt diet on EMT in Dahl salt-sensitive (SS) rats. Twenty-four male SS and consomic SS-13BN rats were randomized to a normal diet or a high-salt diet. After 4 weeks, systolic blood pressure (SBP) and albuminuria were analyzed, and renal fibrosis was histopathologically evaluated. Tubular EMT was evaluated using immunohistochemistry and real-time PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dahl salt-sensitive rats; High salt intake; EMT; Renal fibrosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300223

A meta-analysis of probiotics for preventing necrotizing enterocolitis in preterm neonates
Provedor de dados: 56
Autores: Yang,Y.; Guo,Y.; Kan,Q.; Zhou,X.G.; Zhou,X.Y.; Li,Y.
Necrotizing enterocolitis (NEC) is one of the most common acquired diseases of the gastrointestinal tract in preterm infants. Some randomized, controlled trials (RCTs) have indicated that probiotics may potentially lower the incidence of NEC and mortality. However, debate still remains about the safety of probiotics and their influence on normal infant growth. We performed this meta-analysis to assess the safety and benefits of probiotic supplementation in preterm infants. We searched in PubMed, Embase, and Cochrane databases for English references, and in Wanfang, VIP, and CNKI databases for Chinese references. Ultimately, 27 RCTs (including 9 Chinese articles) were incorporated into this meta-analysis. Relative risk (RR) and weighted mean difference...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Necrotizing enterocolitis; Probiotics; Meta-analysis; Preterm.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900804

Connections: can the 20th century coronary heart disease epidemic reveal something about the 1918 influenza lethality?
Provedor de dados: 56
Autores: Azambuja,M.I.
This essay proposes that the ecologic association shown between the 20th century coronary heart disease epidemic and the 1918 influenza pandemic could shed light on the mechanism associated with the high lethality of the latter. It suggests that an autoimmune interference at the apoB-LDL interface could explain both hypercholesterolemia and inflammation (through interference with the cellular metabolism of arachidonic acid). Autoimmune inflammation, then, would explain the 1950s-60s acute coronary events (coronary thrombosis upon influenza re-infection) and the respiratory failure seen among young adults in 1918. This hypothesis also argues that the lethality of the 1918 pandemic may have not depended so much on the 1918 virus as on an immune vulnerability...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Respiratory distress syndrome; Autoimmunity; Influenza; Coronary disease; Disease susceptibility; Disease outbreaks.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100001

The E3 ligase MuRF2 plays a key role in the functional capacity of skeletal muscle fibroblasts
Provedor de dados: 56
Autores: Silvestre,J.G.; Baptista,I.L.; Silva,W.J.; Cruz,A.; Silva,M.T.; Miyabara,E.H.; Labeit,S.; Moriscot,A.S.
Fibroblasts are a highly heterogeneous population of cells, being found in a large number of different tissues. These cells produce the extracellular matrix, which is essential to preserve structural integrity of connective tissues. Fibroblasts are frequently engaged in migration and remodeling, exerting traction forces in the extracellular matrix, which is crucial for matrix deposition and wound healing. In addition, previous studies performed on primary myoblasts suggest that the E3 ligase MuRF2 might function as a cytoskeleton adaptor. Here, we hypothesized that MuRF2 also plays a functional role in skeletal muscle fibroblasts. We found that skeletal muscle fibroblasts express MuRF2 and its siRNA knock-down promoted decreased fibroblast migration, cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibroblast; Skeletal muscle; Migration; MuRF2; E3 ligase; Wound healing.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900604

Extracellular calcium-sensing receptor: structural and functional features and association with diseases
Provedor de dados: 56
Autores: Hauache,O.M.
The recently cloned extracellular calcium-sensing receptor (CaR) is a G protein-coupled receptor that plays an essential role in the regulation of extracellular calcium homeostasis. This receptor is expressed in all tissues related to this control (parathyroid glands, thyroid C-cells, kidneys, intestine and bones) and also in tissues with apparently no role in the maintenance of extracellular calcium levels, such as brain, skin and pancreas. The CaR amino acid sequence is compatible with three major domains: a long and hydrophilic aminoterminal extracellular domain, where most of the activating and inactivating mutations described to date are located and where the dimerization process occurs, and the agonist-binding site is located, a hydrophobic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium-sensing receptor; Familial hypocalciuric hypercalcemia; Autosomal dominant hypoparathyroidism; Calcimimetic drugs; Calcilytic drugs.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500004

The pathogenesis of tropical spastic paraparesis/human T-cell leukemia type I-associated myelopathy
Provedor de dados: 56
Autores: Casseb,J.; Penalva-de-Oliveira,A.C.
Tropical spastic paraparesis/human T-cell leukemia type I-associated myelopathy (TSP/HAM) is caused by a human T-cell leukemia virus type I (HTLV-I) after a long incubation period. TSP/HAM is characterized by a chronic progressive paraparesis with sphincter disturbances, no/mild sensory loss, the absence of spinal cord compression and seropositivity for HTLV-I antibodies. The pathogenesis of this entity is not completely known and involves a multivariable phenomenon of immune system activation against the presence of HTLV-I antigens, leading to an inflammatory process and demyelination, mainly in the thoracic spinal cord. The current hypothesis about the pathogenesis of TSP/HAM is: 1) presence of HTLV-I antigens in the lumbar spinal cord, noted by an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tropical spastic paraparesis; Human T-cell leukemia type I-associated myelopathy; TSP/HAM.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200001

Respiratory effects of kynurenic acid microinjected into the ventromedullary surface of the rat
Provedor de dados: 56
Autores: Tolentino-Silva,F.P.; Russo,A.K.; Cravo,S.L.; Lopes,O.U.
Several studies demonstrate that, within the ventral medullary surface (VMS), excitatory amino acids are necessary components of the neural circuits involved in the tonic and reflex control of respiration and circulation. In the present study we investigated the cardiorespiratory effects of unilateral microinjections of the broad spectrum glutamate antagonist kynurenic acid (2 nmol/200 nl) along the VMS of urethane-anesthetized rats. Within the VMS only one region was responsive to this drug. This area includes most of the intermediate respiratory area, partially overlapping the rostral ventrolateral medulla (IA/RVL). When microinjected into the IA/RVL, kynurenic acid produced a respiratory depression, without changes in mean arterial pressure or heart...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ventral surface of the medulla; Breathing pattern; Cardiorespiratory control; Kynurenic acid; Rat.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000016

Downregulation of TIM-3 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus
Provedor de dados: 56
Autores: Cai,X.Z.; Huang,W.Y.; Qiao,Y.; Chen,Y.; Du,S.Y.; Chen,D.; Yu,S.; Liu,N.; Dou,L.Y.; Jiang,Y.
The T-cell immunoglobulin and mucin domain (TIM) family is associated with autoimmune diseases, but its expression level in the immune cells of systemic lupus erythematosus (SLE) patients is not known. The aim of this study was to investigate whether the expression of TIM-3 mRNA is associated with pathogenesis of SLE. Quantitative real-time reverse transcription-polymerase chain reaction analysis (qRT-PCR) was used to determine TIM-1, TIM-3, and TIM-4 mRNA expression in peripheral blood mononuclear cells (PBMCs) from 132 patients with SLE and 62 healthy controls. The PBMC surface protein expression of TIMs in PBMCs from 20 SLE patients and 15 healthy controls was assayed by flow cytometry. Only TIM-3 mRNA expression decreased significantly in SLE patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TIM-3; Systemic lupus erythematosus; Lupus nephritis; T-helper cell.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100077

Changes in cell shape, cytoskeletal proteins and adhesion sites of cultured cells after extracellular Ca2+ chelation
Provedor de dados: 56
Autores: Mermelstein,C.S.; Rebello,M.I.L.; Amaral,L.M.; Costa,M.L.
Although much is known about the molecules involved in extracellular Ca2+ regulation, the relationship of the ion with overall cell morphology is not understood. The objective of the present study was to determine the effect of the Ca2+ chelator EGTA on the major cytoskeleton components, at integrin-containing adhesion sites, and their consequences on cell shape. Control mouse cell line C2C12 has a well-spread morphology with long stress fibers running in many different directions, as detected by fluorescence microscopy using rhodamine-phalloidin. In contrast, cells treated with EGTA (1.75 mM in culture medium) for 24 h became bipolar and showed less stress fibers running in one major direction. The adhesion plaque protein alpha5-integrin was detected by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytoskeleton; Extracellular Ca2+; Cell shape; Microfilaments; Microtubules; Integrin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800018

Antidepressant-like effect of Hoodia gordonii in a forced swimming test in mice: evidence for involvement of the monoaminergic system
Provedor de dados: 56
Autores: Citó,M.C.O.; Silva,M.I.G.; Santos,L.K.X.; Fernandes,M.L.; Melo,F.H.C.; Aguiar,J.A.C.; Lopes,I.S.; Sousa,P.B.; Vasconcelos,S.M.M.; Macêdo,D.S.; Sousa,F.C.F.
Hoodia gordonii is a plant species used traditionally in southern Africa to suppress appetite. Recently, it has been associated with a significant increase in blood pressure and pulse rate in women, suggesting sympathomimetic activity. The present study investigated the possible antidepressant-like effects of acute and repeated (15 days) administration of H. gordonii extract (25 and 50 mg/kg, po) to mice exposed to a forced swimming test (FST). Neurochemical analysis of brain monoamines was also carried out to determine the involvement of the monoaminergic system on these effects. Acute administration of H. gordonii decreased the immobility of mice in the FST without accompanying changes in general activity in the open-field test during acute treatment,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hoodia gordonii; Antidepressant action; Monoaminergic system; Forced swimming test.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100057

Effect of Ginkgo biloba on the reproductive outcome and oxidative stress biomarkers of streptozotocin-induced diabetic rats
Provedor de dados: 56
Autores: Rudge,M.V.C.; Damasceno,D.C.; Volpato,G.T.; Almeida,F.C.G.; Calderon,I.M.P.; Lemonica,I.P.
The aim of the present study was to evaluate the effect of Ginkgo biloba treatment (EGb 761, 200 mg kg-1 day-1) administered from day 0 to 20 of pregnancy on maternal reproductive performance and on the maternal and fetal liver antioxidant systems of streptozotocin-induced diabetic Wistar rats. On day 21 of pregnancy, the adult rats (weighing approximately 250 ± 50 g, minimum number = 13/group) were anesthetized to obtain maternal and fetal liver samples for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and total glutathione (GSH-t) determinations. The uterus was weighed with its contents. The diabetic (G3) and treated diabetic (G4) groups of rats presented significant maternal hyperglycemia, reduced term pregnancy rate,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ginkgo biloba; Diabetes mellitus; Pregnancy; Reproductive outcome; Oxidative stress.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800010

Increased immunohistochemical expression of YKL-40 in the spleen of patients with portal hypertension
Provedor de dados: 56
Autores: Wang,Dong; Lu,Jian-Guo; Wang,Qing; Du,Xi-Lin; Dong,Rui; Wang,Peng; Zhao,Lei; Jiang,Xue; Yuan,Li-Juan.
YKL-40 has been identified as a growth factor in connective tissue cells and also a migration factor in vascular smooth muscle cells. To a large extent, the increase of serum YKL-40 is attributed to liver fibrosis and asthma. However, the relationship of the expression and clinical/prognostic significance of YKL-40 to the splenomegaly of patients with portal hypertension is unclear. In the present study, the expression of YKL-40 was studied by immunohistochemistry in 48 splenomegaly tissue samples from patients with portal hypertension and in 14 normal spleen specimens. All specimens were quickly stored at -80°C after resection. Primary antibodies YKL-40 (1:150 dilution, rabbit polyclonal IgG) and MMP-9 (1:200 dilution, rabbit monoclonal IgG) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: YKL-40; MMP-9; Splenomegaly; Fibrosis; Portal hypertension; Immunohistochemistry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300013

Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents)
Provedor de dados: 56
Autores: Calixto,J.B.
This review highlights the current advances in knowledge about the safety, efficacy, quality control, marketing and regulatory aspects of botanical medicines. Phytotherapeutic agents are standardized herbal preparations consisting of complex mixtures of one or more plants which contain as active ingredients plant parts or plant material in the crude or processed state. A marked growth in the worldwide phytotherapeutic market has occurred over the last 15 years. For the European and USA markets alone, this will reach about $7 billion and $5 billion per annum, respectively, in 1999, and has thus attracted the interest of most large pharmaceutical companies. Insufficient data exist for most plants to guarantee their quality, efficacy and safety. The idea that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbal medicine; Efficacy; Safety; Quality control; Market; Regulatory guidelines.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200004

Combined aliskiren and L-arginine treatment has antihypertensive effects and prevents vascular endothelial dysfunction in a model of renovascular hypertension
Provedor de dados: 56
Autores: Santuzzi,C.H.; Tiradentes,R.V.; Mengal,V.; Claudio,E.R.G.; Mauad,H.; Gouvea,S.A.; Abreu,G.R.
Angiotensin II is a key player in the pathogenesis of renovascular hypertension, a condition associated with endothelial dysfunction. We investigated aliskiren (ALSK) and L-arginine treatment both alone and in combination on blood pressure (BP), and vascular reactivity in aortic rings. Hypertension was induced in 40 male Wistar rats by clipping the left renal artery. Animals were divided into Sham, 2-kidney, 1-clip (2K1C) hypertension, 2K1C+ALSK (ALSK), 2K1C+L-arginine (L-arg), and 2K1C+ALSK+L-arginine (ALSK+L-arg) treatment groups. For 4 weeks, BP was monitored and endothelium-dependent and independent vasoconstriction and relaxation were assessed in aortic rings. ALSK+L-arg reduced BP and the contractile response to phenylephrine and improved...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2K1C hypertension; Endothelial dysfunction; Aliskiren; L-arginine; RAAS and oxidative stress.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100065

Electrophysiological evidence for the presence of NR2C subunits of N-methyl-D-aspartate receptors in rat neurons of the nucleus tractus solitarius
Provedor de dados: 56
Autores: Baptista,V.; Ogawa,W.N.; Aguiar,J.F.; Varanda,W.A.
The nucleus tractus solitarius (NTS) plays an important role in the control of autonomic reflex functions. Glutamate, acting on N-methyl-D-aspartate (NMDA) and non-NMDA ionotropic receptors, is the major neurotransmitter in this nucleus, and the relative contribution of each receptor to signal transmission is unclear. We have examined NMDA excitatory postsynaptic currents (NMDA-EPSCs) in the subpostremal NTS using the whole cell patch clamp technique on a transverse brainstem slice preparation. The NMDA-EPSCs were evoked by stimulation of the solitary tract over a range of membrane potentials. The NMDA-EPSCs, isolated pharmacologically, presented the characteristic outward rectification and were completely blocked by 50 µM DL-2-amino-5-phosphonopentanoic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nucleus tractus solitarius; Electrophysiology; Synapse; NMDA receptors; NR2C subunits.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100016

Comparison of etoricoxib and indomethacin for the treatment of experimental periodontitis in rats
Provedor de dados: 56
Autores: Azoubel,M.C.F.; Menezes,A.M.A.; Bezerra,D.; Oriá,R.B.; Ribeiro,R.A.; Brito,G.A.C.
We investigated the effect of etoricoxib, a selective cyclooxygenase-2 inhibitor, and indomethacin, a non-selective cyclooxygenase inhibitor, on experimental periodontitis, and compared their gastrointestinal side effects. A ligature was placed around the second upper left molars of female Wistar rats (160 to 200 g). Animals (6 per group) were treated daily with oral doses of 3 or 9 mg/kg etoricoxib, 5 mg/kg indomethacin, or 0.2 mL saline, starting 5 days after the induction of periodontitis, when bone resorption was detected, until the sacrifice on the 11th day. The weight and survival rate were monitored. Alveolar bone loss (ABL) was measured as the sum of distances between the cusp tips and the alveolar bone. The gastric mucosa was examined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alveolar bone loss; Inflammation; Periodontitis; Cyclooxygenase inhibitors; Etoricoxib; Indomethacin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100015

The brain decade in debate: VI. Sensory and motor maps: dynamics and plasticity
Provedor de dados: 56
Autores: Das,A.; Franca,J.G.; Gattass,R.; Kaas,J.H.; Nicolelis,M.A.L.; Timo-Iaria,C.; Vargas,C.D.; Weinberger,N.M.; Volchan,E.
This article is an edited transcription of a virtual symposium promoted by the Brazilian Society of Neuroscience and Behavior (SBNeC). Although the dynamics of sensory and motor representations have been one of the most studied features of the central nervous system, the actual mechanisms of brain plasticity that underlie the dynamic nature of sensory and motor maps are not entirely unraveled. Our discussion began with the notion that the processing of sensory information depends on many different cortical areas. Some of them are arranged topographically and others have non-topographic (analytical) properties. Besides a sensory component, every cortical area has an efferent output that can be mapped and can influence motor behavior. Although new behaviors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visual; Somatosensory; Auditory; Motor; Cortex; Topography; Learning; Synesthesia.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200001

Insulin secretion, insulin sensitivity, and hepatic insulin extraction in first-degree relatives of type 2 diabetic patients
Provedor de dados: 56
Autores: Pimenta,W.P.; Santos,M.L.; Cruz,N.S.; Aragon,F.F.; Padovani,C.R.; Gerich,J.E.
To identify early metabolic abnormalities in type 2 diabetes mellitus, we measured insulin secretion, sensitivity to insulin, and hepatic insulin extraction in 48 healthy normal glucose-tolerant Brazilians, first-degree relatives of type 2 diabetic patients (FH+). Each individual was matched for sex, age, weight, and body fat distribution with a person without history of type 2 diabetes (FH-). Both groups were submitted to a hyperglycemic clamp procedure (180 mg/dl). Insulin release was evaluated in its two phases. The first was calculated as the sum of plasma insulin at 2.5, 5.0, 7.5, and 10.0 min after the beginning of glucose infusion, and the second as the mean plasma insulin level in the third hour of the clamp procedure. Insulin sensitivity index...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes; Insulin secretion; Insulin sensitivity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300003

Gender-related pathways for behavior problems in the offspring of alcoholic fathers
Provedor de dados: 56
Autores: Furtado,E.F.; Laucht,M.; Schmidt,M.H.
The objective of the present study was to examine gender differences in the influence of paternal alcoholism on children's social-emotional development and to determine whether paternal alcoholism is associated with a greater number of externalizing symptoms in the male offspring. From the Mannheim Study of Risk Children, an ongoing longitudinal study of a high-risk population, the developmental data of 219 children [193 (95 boys and 98 girls) of non-alcoholic fathers, non-COAs, and 26 (14 boys, 12 girls) of alcoholic fathers, COAs] were analyzed from birth to the age of 11 years. Paternal alcoholism was defined according to the ICD-10 categories of alcohol dependence and harmful use. Socio-demographic data, cognitive development, number and severity of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Longitudinal study; Paternal alcohol disorder; Child development; Psychiatric disorder; Gender differences; Externalizing disorders.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500013

Possible benefit of consolidation therapy with high-dose cytarabine on overall survival of adults with non-promyelocytic acute myeloid leukemia
Provedor de dados: 56
Autores: Azevedo,M.C.; Velloso,E.D.R.P.; Buccheri,V.; Chamone,D.A.F.; Dorlhiac-Llacer,P.E.
In adults with non-promyelocytic acute myeloid leukemia (AML), high-dose cytarabine consolidation therapy has been shown to influence survival in selected patients, although the appropriate doses and schemes have not been defined. We evaluated survival after calculating the actual dose of cytarabine that patients received for consolidation therapy and divided them into 3 groups according to dose. We conducted a single-center, retrospective study involving 311 non-promyelocytic AML patients with a median age of 36 years (16-79 years) who received curative treatment between 1978 and 2007. The 131 patients who received cytarabine consolidation were assigned to study groups by their cytarabine dose protocol. Group 1 (n=69) received &lt;1.5 g/m2 every 12 h on 3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myeloid leukemia; Chemotherapy; Consolidation therapy; High-dose cytarabine; Prognosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200178

Combination therapy of orally administered glycyrrhizin and UVB improved active-stage generalized vitiligo
Provedor de dados: 56
Autores: Mou,K.H.; Han,D.; Liu,W.L.; Li,P.
Glycyrrhizin has been used clinically for several years due to its beneficial effect on immunoglobulin E (IgE)-induced allergic diseases, alopecia areata and psoriasis. In this study, glycyrrhizin, ultraviolet B light (UVB) or a combination of both were used to treat active-stage generalized vitiligo. One hundred and forty-four patients between the ages of 3 and 48 years were divided into three groups: group A received oral compound glycyrrhizin (OCG); group B received UVB applications twice weekly, and group C received OCG+UVB. Follow-ups were performed at 2, 4, and 6 months after the treatment was initiated. The Vitiligo Area Scoring Index (VASI) and the Vitiligo Disease Activity (VIDA) instrument were used to assess the affected body surface, at each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycyrrhizin; UVB; Generalized vitiligo; Disease stage; Active stage.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800605

Overview of Zika virus (ZIKV) infection in regards to the Brazilian epidemic
Provedor de dados: 56
Autores: Slavov,S.N.; Otaguiri,K.K.; Kashima,S.; Covas,D.T.
Zika virus (ZIKV), a mosquito-borne flavivirus, belongs to the Flaviviridae family, genus Flavivirus. ZIKV was initially isolated in 1947 from a sentinel monkey in the Zika forest, Uganda. Little clinical importance was attributed to ZIKV, once only few symptomatic cases were reported in some African and Southeast Asiatic countries. This situation changed in 2007, when a large outbreak was registered on the Yap Island, Micronesia, caused by the Asian ZIKV lineage. Between 2013 and 2014, ZIKV spread explosively and caused many outbreaks in different islands of the Southern Pacific Ocean and in 2015 autochthonous transmission was reported in Brazil. Currently, Brazil is the country with the highest number of ZIKV-positive cases in Latin America. Moreover,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zika virus; ZIKV; Transmission; Epidemiology; Congenital infection; Brazil.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500201

Modulation of extracellular matrix by nutritional hepatotrophic factors in thioacetamide-induced liver cirrhosis in the rat
Provedor de dados: 56
Autores: Guerra,R.R.; Trotta,M.R.; Parra,O.M.; Avanzo,J.L.; Bateman,A.; Aloia,T.P.A.; Dagli,M.L.Z.; Hernandez-Blazquez,F.J.
Nutritional substances associated to some hormones enhance liver regeneration when injected intraperitoneally, being denominated hepatotrophic factors (HF). Here we verified if a solution of HF (glucose, vitamins, salts, amino acids, glucagon, insulin, and triiodothyronine) can revert liver cirrhosis and how some extracellular matrices are affected. Cirrhosis was induced for 14 weeks in 45 female Wistar rats (200 mg) by intraperitoneal injections of thioacetamide (200 mg/kg). Twenty-five rats received intraperitoneal HF twice a day for 10 days (40 mL·kg-1·day-1) and 20 rats received physiological saline. Fifteen rats were used as control. The HF applied to cirrhotic rats significantly: a) reduced the relative mRNA expression of the genes: Col-α1 (-53%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver cirrhosis; Hepatotrophic factors; Collagen; Matrix modulation; Progranulin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100005

Reference values for lung function tests: I. Static volumes
Provedor de dados: 56
Autores: Neder,J.A.; Andreoni,S.; Castelo-Filho,A.; Nery,L.E.
Static lung volume (LV) measurements have a number of clinical and research applications; however, no previous studies have provided reference values for such tests using a healthy sample of the adult Brazilian population. With this as our main purpose, we prospectively evaluated 100 non-smoking subjects (50 males and 50 females), 20 to 80 years old, randomly selected from more than 8,000 individuals. Gender-specific linear prediction equations were developed by multiple regression analysis with total lung capacity (TLC), functional residual capacity (FRC), residual volume (RV), RV/TLC ratio and inspiratory capacity (IC) as dependent variables, and with age, height, weight, lean body mass and indexes of physical fitness as independent ones. Simpler...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung volumes; Pulmonary function tests; Pulmonary diseases.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600006

Genetic characterization of dengue virus type 3 isolates in the State of Rio de Janeiro, 2001
Provedor de dados: 56
Autores: Miagostovich,M.P.; Santos,F.B. dos; Simone,T.S. de; Costa,E.V.; Filippis,A.M.B.; Schatzmayr,H.G.; Nogueira,R.M.R.
The genetic characterization of dengue virus type 3 (DEN-3) strains isolated from autochthonous cases in the State of Rio de Janeiro, Brazil, in 2001 is presented. Restriction site-specific (RSS)-PCR performed on 22 strains classified the Brazilian DEN-3 viruses as subtype C, a subtype that contains viruses from Sri Lanka, India, Africa and recent isolates from Central America. Nucleic acid sequencing (positions 278 to 2550) of one DEN-3 strain confirmed the origin of these strains, since genotype III - classified by sequencing - and RSS-PCR subtype C are correlated. This genetic subtype has been associated with hemorrhagic dengue epidemics and the information provided here could be useful to implement appropriate prevention and control measures.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dengue viruses type 3; Sequencing; RSS-PCR; Rio de Janeiro; Brazil.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800002

Leptin levels in different forms of Chagas' disease
Provedor de dados: 56
Autores: Fernandes,F.; Dantas,S.; Ianni,B.M.; Ramires,F.J.A.; Buck,P.; Salemi,V.M.C.; Lopes,H.F.; Mady,C.
Leptin is produced primarily by adipocytes. Although originally associated with the central regulation of satiety and energy metabolism, increasing evidence indicates that leptin may be an important mediator in cardiovascular pathophysiology. The aim of the present study was to investigate plasma leptin levels in patient with Chagas' heart disease and their relation to different forms of the disease. We studied 52 chagasic patients and 30 controls matched for age and body mass index. All subjects underwent anthropometric, leptin and N-terminal pro-brain natriuretic peptide (NT-proBNP) measurements and were evaluated by echocardiography, 12-lead electrocardiogram (ECG), and chest X-ray. All patients had fasting blood samples taken between 8:00 and 9:00 am....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chagas' disease; Leptin; Heart failure; Left ventricular failure.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200006

Residence of liquids in the infra-junctional portion of the proximal stomach in patients with gastroesophageal reflux disease
Provedor de dados: 56
Autores: Barbieri,C.L.A.; Troncon,L.E.A.; Herculano Jr.,J.R.L.; Aprile,L.R.O.; Dantas,R.O.
Patients with gastroesophageal reflux disease may have disturbances of gastric motility, which could play a role in the pathophysiology of the disease. Recent studies have suggested that the gastric region just below the gastroesophageal junction may have a distinct physiological behavior. We determined whether patients with gastroesophageal reflux disease have abnormal residence of food in the infra-junctional portion of the stomach after ingesting a liquid nutrient meal. Fasted adult patients with reflux disease (N = 11) and healthy volunteers (N = 10) ingested a liquid meal (320 ml; 437 kcal) labeled with 99m technetium-phytate and their total gastric emptying half-time and regional emptying from the stomach infra-junctional region were determined. In 8...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastroesophageal reflux disease; Gastric emptying; Gastric motility; Intragastric distribution; Proximal stomach.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900012

Antihypertensive effects of angiotensin-(1-7)
Provedor de dados: 56
Autores: Chappell,M.C.; Iyer,S.N.; Diz,D.I.; Ferrario,C.M.
Accumulating evidence suggests that angiotensin-(1-7) (Ang-(1-7)) is an important component of the renin-angiotensin system and that the actions of the peptide may either contribute to or oppose those of Ang II. Ang-(1-7) can be converted directly from Ang I bypassing prerequisite formation of Ang II. Formation of Ang-(1-7) is under the control of at least three endopeptidases depending on the tissue compartment and include neprilysin, thimet oligopeptidase and prolyl oligopeptidase. Both neprilysin and thimet oligopeptidase are also involved in the metabolism of bradykinin and the atrial natriuretic peptide. Moreover, recent studies suggest that in addition to Ang I and bradykinin, Ang-(1-7) is an endogenous substrate for angiotensin converting enzyme....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin-(1-7); Angiotensin II; Hypertension.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900014

L-histidine reduces inhibitory avoidance in Carassius auratus submitted to cerebellar ablation
Provedor de dados: 56
Autores: Garção,D.C.; Mattioli,R.
The effect of post-training treatment with L-histidine (LH) on the memory consolidation of inhibitory avoidance was investigated in Carassius auratus submitted to cerebellar ablation. The inhibitory avoidance procedure included 3 days: one habituation day, one training day (5 trials, T1-T5) and one test day. On the training day, each fish was placed individually in a white compartment separated from a black compartment by a sliding door. When the fish crossed into the black compartment, a weight was dropped in front of it (aversive stimulus) and the time to cross was recorded. Saline or LH (100 mg/kg) was injected intraperitoneally 10 min after the trials. Data were log10 transformed and analyzed by ANOVA and the Student-Newman-Keuls test (P &lt; 0.05). In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inhibitory avoidance; L-histidine; Cerebellar ablation; Medial telencephalic pallium; Carassius auratus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100019

Mechanisms in anti-inflammation and resolution: the role of lipoxins and aspirin-triggered lipoxins
Provedor de dados: 56
Autores: Fierro,I.M.; Serhan,C.N.
Multicellular host responses to infection, injury or inflammatory stimuli lead to the formation of a broad range of chemical mediators by the host. The integrated response of the host is essential to health and disease; thus it is important to achieve a more complete understanding of the molecular and cellular events governing the formation and actions of endogenous mediators of resolution that appear to control the duration of inflammation. Lipoxins are trihydroxytetraene-containing lipid mediators that can be formed during cell-cell interactions and are predominantly counterregulators of some well-known mediators of inflammation. Since this circuit of lipoxin formation and action appears to be of physiological relevance for the resolution of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Lipoxins; Resolution.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500002

Gentle handling temporarily increases c-Fos in the substantia nigra pars compacta
Provedor de dados: 56
Autores: Santos,C.A.; Andersen,M.L.; Lima,M.M.S.; Tufik,S.
Dopaminergic neurotransmission is involved in the regulation of sleep. In particular, the nigrostriatal pathway is an important center of sleep regulation. We hypothesized that dopaminergic neurons located in substantia nigra pars compacta (SNpc) could be activated by gentle handling, a method to obtain sleep deprivation (SD). Adult male C57/BL6J mice (N = 5/group) were distributed into non-SD (NSD) or SD groups. SD animals were subjected to SD once for 1 or 3 h by gentle handling. Two experiments were performed. The first determined the activation of SNpc neurons after SD, and the second examined the same parameters after pharmacologically induced dopaminergic depletion using intraperitoneal reserpine (2 mg/kg). After 1 or 3 h, SD and NSD mice were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sleep deprivation; Dopamine; C-Fos; Substantia nigra pars compacta; Gentle handling of mice.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000015

Effect of fatty acids on leukocyte function
Provedor de dados: 56
Autores: Pompéia,C.; Lopes,L.R.; Miyasaka,C.K.; Procópio,J.; Sannomiya,P.; Curi,R.
Fatty acids have various effects on immune and inflammatory responses, acting as intracellular and intercellular mediators. Polyunsaturated fatty acids (PUFAs) of the omega-3 family have overall suppressive effects, inhibiting lymphocyte proliferation, antibody and cytokine production, adhesion molecule expression, natural killer cell activity and triggering cell death. The omega-6 PUFAs have both inhibitory and stimulatory effects. The most studied of these is arachidonic acid that can be oxidized to eicosanoids, such as prostaglandins, leukotrienes and thromboxanes, all of which are potent mediators of inflammation. Nevertheless, it has been found that many of the effects of PUFA on immune and inflammatory responses are not dependent on eicosanoid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatty acids; Immune system; Inflammation; Dietary lipids; Leukocytes.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100001

In vitro antimicrobial activity of a new series of 1,4-naphthoquinones
Provedor de dados: 56
Autores: Riffel,A.; Medina,L.F.; Stefani,V.; Santos,R.C.; Bizani,D.; Brandelli,A.
The antibacterial activity of a series of 1,4-naphthoquinones was demonstrated. Disk diffusion tests were carried out against several Gram-positive and Gram-negative bacteria. The compound 5-amino-8-hydroxy-1,4-naphthoquinone was the most effective, presenting inhibition zones measuring 20 mm against staphylococci, streptococci and bacilli at 50 µg/ml. Methicillin-resistant Staphylococcus aureus and several clinical isolates of this bacterium were also inhibited. Naphthazarin, 5-acetamido-8-hydroxy-1,4-naphthoquinone, and 2,3-diamino-1,4-naphthoquinone were the next most active compounds. The minimal inhibitory concentration of the active compounds was determined against S. aureus, ranging from 30 to 125 µg/ml. All compounds presented a minimal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5-Amino-8-hydroxy-1; 4-naphthoquinone Antimicrobial agent Staphylococcus aureus Quinone Naphthazarin 5-Acetamido-8-hydroxy-1; 4-naphthoquinone 2; 3-Diamino-1; 4-naphthoquinone.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700008

Quality of life and adherence to treatment in early-treated Brazilian phenylketonuria pediatric patients
Provedor de dados: 56
Autores: Vieira Neto,E.; Maia Filho,H.S.; Monteiro,C.B.; Carvalho,L.M.; Tonon,T.; Vanz,A.P.; Schwartz,I.V.D.; Ribeiro,M.G.
Early dietary treatment of phenylketonuria (PKU), an inborn error of phenylalanine (Phe) metabolism, results in normal cognitive development. Although health-related quality of life (HRQoL) of PKU patients has been reported as unaffected in high-income countries, there are scarce data concerning HRQoL and adherence to treatment of PKU children and adolescents from Brazil. The present study compared HRQoL scores in core dimensions of Brazilian early-treated PKU pediatric patients with those of a reference population, and explored possible relationships between adherence to treatment and HRQoL. Early-treated PKU pediatric patient HRQoL was evaluated by self- and parent-proxy reports of the Pediatric Quality of Life Inventory (PedsQL) core scales. Adherence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenylketonuria; Quality of life; Questionnaires; Patient compliance; Diet therapy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200613

Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells
Provedor de dados: 56
Autores: Hu,T.; Shi,J.; Jiao,X.; Zhou,J.; Yin,X.
Phosphatidylserine (PS) exposure occurs during the cell death program and fluorescein-labeled lactadherin permits the detection of PS exposure earlier than annexin V in suspended cell lines. Adherent cell lines were studied for this apoptosis-associated phenomenon to determine if PS probing methods are reliable because specific membrane damage may occur during harvesting. Apoptosis was induced in the human tongue squamous carcinoma cell line (Tca8113) and the adenoid cystic carcinoma cell line (ACC-2) by arsenic trioxide. Cells were harvested with a modified procedure and labeled with lactadherin and/or annexin V. PS exposure was localized by confocal microscopy and apoptosis was quantified by flow cytometry. The detachment procedure without trypsinization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adherent cells; Annexin V; Apoptosis; Flow cytometry; Lactadherin.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900002

Proximal tubular dysfunction as an indicator of chronic graft dysfunction
Provedor de dados: 56
Autores: Câmara,N.O.S.; Williams Jr.,W.W.; Pacheco-Silva,A.
New strategies are being devised to limit the impact of renal sclerosis on graft function. Individualization of immunosuppression, specifically the interruption of calcineurin-inhibitors has been tried in order to promote better graft survival once chronic graft dysfunction has been established. However, the long-term impact of these approaches is still not totally clear. Nevertheless, patients at higher risk for tubular atrophy and interstitial fibrosis (TA/IF) development should be carefully monitored for tubular function as well as glomerular performance. Since tubular-interstitial impairment is an early event in TA/IF pathogenesis and associated with graft function, it seems reasonable that strategies directed at assessing tubular structural integrity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal transplantation; Tubular proteins; Proximal tubular dysfunction; Chronic allograft nephropathy; Retinol binding protein.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300003

Correlation of discocyte frequency and ATP concentration in preserved blood: A morphological indicator of red blood cell viability
Provedor de dados: 56
Red blood cells (RBC) are viable if kept in an adequate preservative solution, although gradual changes in morphology and metabolism may occur. There is a gradual decrease in adenosine-5'-triphosphate (ATP) concentration, pH, glucose consumption, and enzyme activity during preservation. The normal discocyte shapes are initially replaced by echinocytes and stomatocytes and, at final stages, by spherocytes, the last step before splenic sequestration. Post-transfusional survival has been correlated with the ATP concentration. RBC preserved in ADSOL, a solution containing adenine, dextrose, sodium chloride, and mannitol, are viable for transfusion for up to 6 weeks. Erythrocytes from 10 blood units taken from healthy adult donors were preserved for 12 weeks in...
Palavras-chave: Red blood cells; Adenosine-5'-triphosphate; Blood transfusion.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600007

The renal and hepatic distribution of Bence Jones proteins depends on glycosylation: a scintigraphic study in rats
Provedor de dados: 56
The aim of the present study was to evaluate renal and liver distribution of two monoclonal immunoglobulin light chains. The chains were purified individually from the urine of patients with multiple myeloma and characterized as lambda light chains with a molecular mass of 28 kDa. They were named BJg (high amount of galactose residues exposed) and BJs (sialic acid residues exposed) on the basis of carbohydrate content. A scintigraphic study was performed on male Wistar rats weighing 250 g for 60 min after iv administration of 1 mg of each protein (7.4 MBq), as the intact proteins and also after carbohydrate oxidation. Images were obtained with a Siemens gamma camera with a high-resolution collimator and processed with a MicroDelta system. Hepatic and renal...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bence Jones proteins; Scintigraphic study; Multiple myeloma; Nephrotoxicity.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700008

Association of urinary 90 kDa angiotensin- converting enzyme with family history of hypertension and endothelial function in normotensive individuals
Provedor de dados: 56
Autores: Teixeira,A.M.S.; Plavnik,F.L.; Fernandes,F.B.; Marson,O.; Christofalo,D.M.J.; Ajzen,S.A.; Sesso,R.; Franco,M.C.; Casarini,D.E.
We described angiotensin-I-converting enzyme (ACE) isoforms with molecular masses of 190, 90, and 65 kDa in the urine of normotensive offspring of hypertensive subjects. Since they did not appear in equal amounts, we suggested that 90 kDa ACE might be a marker for hypertension. We evaluated the endothelial response in normotensive offspring with or without family history of hypertension and its association with the 90 kDa ACE in urine. Thirty-five normotensive subjects with a known family history of hypertension and 20 subjects without a family history of hypertension, matched for age, sex, body weight, and blood pressure, were included in the study. Endothelial function was assessed by ultrasound and a sample of urine was collected for determination of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelial dysfunction; Angiotensin-I-converting enzyme; Hypertension; Normotension; N-domain angiotensin- converting enzyme.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500002

Long-lasting mnemotropic effect of substance P and its N-terminal fragment (SP1-7) on avoidance learning
Provedor de dados: 56
We investigated the long-lasting effect of peripheral injection of the neuropeptide substance P (SP) and of some N- or C-terminal SP fragments (SPN and SPC, respectively) on retention test performance of avoidance learning. Male Wistar rats (220 to 280 g) were trained in an inhibitory step-down avoidance task and tested 24 h or 21 days later. Immediately after the training trial rats received an intraperitoneal injection of SP (50 µg/kg), SPN 1-7 (167 µg/kg) or SPC 7-11 (134 µg/kg). Control groups were injected with vehicle or SP 5 h after the training trial. The immediate post-training administration of SP and SPN, but not SPC, facilitated avoidance behavior in rats tested 24 h or 21 days later, i.e., the retention test latencies of the SP and SPN groups...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Substance P; Substance P fragments; Avoidance conditioning; Long-lasting memory.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200011

Sympathetic activation in rats with L-NAME-induced hypertension
Provedor de dados: 56
Autores: Biancardi,V.C.; Bergamaschi,C.T.; Lopes,O.U.; Campos,R.R.
We evaluated the hemodynamic pattern and the contribution of the sympathetic nervous system in conscious and anesthetized (1.4 g/kg urethane, iv) Wistar rats with L-NAME-induced hypertension (20 mg/kg daily). The basal hemodynamic profile was similar for hypertensive animals, conscious (N = 12) or anesthetized (N = 12) treated with L-NAME for 2 or 7 days: increase of total peripheral resistance associated with a decrease of cardiac output (CO) compared to normotensive animals, conscious (N = 14) or anesthetized (N = 14). Sympathetic blockade with hexamethonium essentially caused a decrease in total peripheral resistance in hypertensive animals (conscious, 2 days: from (means ± SEM) 2.47 ± 0.08 to 2.14 ± 0.07; conscious, 7 days: from 2.85 ± 0.13 to 2.07 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-NAME hypertension; Sympathetic nervous system; Nitric oxide synthase blockade; Total peripheral resistance.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300016

Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI)6]+
Provedor de dados: 56
Autores: Faria,D.P.; Buchpiguel,C.A.; Marques,F.L.N.
Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [99mTc(MIBI)6]+ as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol:chloroform (25:75), Whatman 3MM paper and ethanol:chloroform...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopharmaceuticals; Technetium-99m; Quality control; Chromatography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000007

Down-regulation of the cardiac sarcoplasmic reticulum ryanodine channel in severely food-restricted rats
Provedor de dados: 56
Autores: Vizotto,V.A.; Carvalho,R.F.; Sugizaki,M.M.; Lima,A.P.; Aragon,F.F.; Padovani,C.R.; Castro,A.V.B.; Dal Pai-Silva,M.; Nogueira,C.R.; Cicogna,A.C.
We have shown that myocardial dysfunction induced by food restriction is related to calcium handling. Although cardiac function is depressed in food-restricted animals, there is limited information about the molecular mechanisms that lead to this abnormality. The present study evaluated the effects of food restriction on calcium cycling, focusing on sarcoplasmic Ca2+-ATPase (SERCA2), phospholamban (PLB), and ryanodine channel (RYR2) mRNA expressions in rat myocardium. Male Wistar-Kyoto rats, 60 days old, were submitted to ad libitum feeding (control rats) or 50% diet restriction for 90 days. The levels of left ventricle SERCA2, PLB, and RYR2 were measured using semi-quantitative RT-PCR. Body and ventricular weights were reduced in 50% food-restricted...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Food restriction; SERCA2; Ryanodine channel; Phospholamban; MRNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100004

In situ variation of cervical mucus pH during exposure to atmospheric air
Provedor de dados: 56
Autores: Correa,C.H.M.; Mattos,A.L.G.; Ferrari,A.N.
The objective of the present study was to determine if exposure of cervical mucus to air during specular examination could modify mucus pH. Detection of changes is justified because of their possible interference with sperm-mucus interaction, since an acidic pH is unfavorable to sperm penetration and is associated with infertility due to the cervical factor. Twenty women with good quality mucus were evaluated. pH measurements of ecto- and endocervical mucus were made in situ using a glass electrode after 0-, 5- and 10-min exposure to air. There was a progressive alkalinization of mucus pH. Mean values of ectocervical mucus pH were 6.91, 7.16 and 7.27, while mean values of endocervical mucus pH were 7.09, 7.34 and 7.46 at 0, 5 and 10 min, respectively....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cervical mucus pH; Infertility; Postcoital test.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600011

Effect of race, genetic population structure, and genetic models in two-locus association studies: clustering of functional renin-angiotensin system gene variants in hypertension association studies
Provedor de dados: 56
Autores: Pereira,A.C.; Mota,G.A.; Benseñor,I.; Lotufo,P.A.; Krieger,J.E.
Previous genetic association studies have overlooked the potential for biased results when analyzing different population structures in ethnically diverse populations. The purpose of the present study was to quantify this bias in two-locus association studies conducted on an admixtured urban population. We studied the genetic structure distribution of angiotensin-converting enzyme insertion/deletion (ACE I/D) and angiotensinogen methionine/threonine (M/T) polymorphisms in 382 subjects from three subgroups in a highly admixtured urban population. Group I included 150 white subjects; group II, 142 mulatto subjects, and group III, 90 black subjects. We conducted sample size simulation studies using these data in different genetic models of gene action and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Association study; Genetics; Angiotensin-converting enzyme; ACE; Angiotensinogen.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100008

Shared control of maltose and trehalose utilization in Candida utilis
Provedor de dados: 56
Autores: Rolim,M.F.; de Araujo,P.S.; Panek,A.D.; Paschoalin,V.M.F.; Silva,J.T.
Trehalose biosynthesis and its hydrolysis have been extensively studied in yeast, but few reports have addressed the catabolism of exogenously supplied trehalose. Here we report the catabolism of exogenous trehalose by Candida utilis. In contrast to the biphasic growth in glucose, the growth of C. utilis in a mineral medium with trehalose as the sole carbon and energy source is aerobic and exhibits the Kluyver effect. Trehalose is transported into the cell by an inducible trehalose transporter (K M of 8 mM and V MAX of 1.8 µmol trehalose min-1 mg cell (dry weight)-1. The activity of the trehalose transporter is high in cells growing in media containing trehalose or maltose and very low or absent during the growth in glucose or glycerol. Similarly, total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trehalose; Trehalase; Sugar transporter; Candida utilis; Yeast.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700002

Impact of congenital calcitonin deficiency due to dysgenetic hypothyroidism on bone mineral density
Provedor de dados: 56
Autores: Daripa,M.; Paula,F.J.A.; Rufino,A.C.B.; Foss,M.C.
The objective of the present study was to determine the effect of chronic calcitonin deficiency on bone mass development. The results of 11 patients with thyroid dysgenesis (TD) were compared to those of 17 normal individuals (C) and of 9 patients with other forms of hypothyroidism (OH): 4 with hypothyroidism due to inborn errors of thyroid hormone synthesis and 5 with Hashimoto's thyroiditis. The subjects received an intravenous calcium stimulus and blood was collected for the determination of ionized calcium (Ca2+), calcitonin, and intact parathyroid hormone. Bone mineral density (BMD) was determined by dual-energy X-ray absorptiometry. After calcium administration the levels of Ca2+ in the two groups of hypothyroidism were significantly higher than in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoporosis; Hypothyroidism; Calcitonin; Bone mineral density.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100009

Neoadjuvant chemotherapy with trastuzumab in HER2-positive breast cancer: pathologic complete response rate, predictive and prognostic factors
Provedor de dados: 56
Autores: Buzatto,I.P.C.; Ribeiro-Silva,A.; Andrade,J.M.; Carrara,H.H.A.; Silveira,W.A.; Tiezzi,D.G.
The purpose of this study was to retrospectively review the pathologic complete response (pCR) rate from patients (n=86) with stage II and III HER2-positive breast cancer treated with neoadjuvant chemotherapy at our institution from 2008 to 2013 and to determine possible predictive and prognostic factors. Immunohistochemistry for hormone receptors and Ki-67 was carried out. Clinical and pathological features were analyzed as predictive factors of response to therapy. For survival analysis, we used Kaplan-Meier curves to estimate 5-year survival rates and the log-rank test to compare the curves. The addition of trastuzumab to neoadjuvant chemotherapy significantly improved pCR rate from 4.8 to 46.8%, regardless of the number of preoperative trastuzumab...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neoadjuvant chemotherapy; Trastuzumab; Pathologic complete response; Predictive factors of response; Prognosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200702

Preliminary data on the prevalence of psychiatric disorders in Brazilian male and female juvenile delinquents
Provedor de dados: 56
Autores: Andrade,R.C.; Silva,V.A.; Assumpção Jr.,F.B.
The aim of the present investigation was to study the prevalence of psychiatric disorders in a sample of delinquent adolescents of both genders and to compare the prevalence between genders. A total of 116 adolescents (99 males and 17 females) aged 12 to 19 on parole in the State of Rio de Janeiro were interviewed using the screening interview based on the Schedule for Affective Disorders and Schizophrenia for School-Age Children - Present and Lifetime (KSADS-PL). Data were collected between May 2002 and January 2003. Of 373 male and 58 female adolescents present in May 2002 in the largest institution that gives assistance to adolescents on parole in the city of Rio de Janeiro, 119 subjects were assessed (three of them refused to participate). Their...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Delinquency; Conduct disorder; Psychiatry disorders; Adolescents; Parolees; Illicit drug use.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800005

Mitochondrial DNA synthesis studied autoradiographically in various cell types in vivo
Provedor de dados: 56
Autores: Korr,H.; Kurz,C.; Seidler,T.O.; Sommer,D.; Schmitz,C.
It is generally accepted that mitochondria are able to proliferate even in postmitotic cells due to their natural turnover and also to satisfy increased cell energy requirements. However, no detailed studies are available, particularly with respect to specific cell types. Since [3H]-thymidine is incorporated not only into nuclear (n) DNA but also into the DNA of cytoplasmic mitochondria, an autoradiographic approach was developed at the light microscopy level in order to study basic questions of mitochondrial (mt) proliferation in organs of rodents in situ via the cytoplasmic incorporation of [3H]-thymidine injected into the animals 1 h before sacrifice. Experiments carried out on mice after X-irradiation showed that cytoplasmic labeling was not due to a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Autoradiography; Mitochondrial DNA synthesis; Turnover of mitochondria; Unscheduled DNA synthesis.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200012

Identification of the main generator source of longitudinal muscle contraction in the earthworm ventral nerve cord
Provedor de dados: 56
Autores: Chang,Y.C.; Assmé,Z.; Caffaro,E.C.L.; Bartoszeck,A.B.
The main generator source of a longitudinal muscle contraction was identified as an M (mechanical-stimulus-sensitive) circuit composed of a presynaptic M-1 neuron and a postsynaptic M-2 neuron in the ventral nerve cord of the earthworm, Amynthas hawayanus, by simultaneous intracellular response recording and Lucifer Yellow-CH injection with two microelectrodes. Five-peaked responses were evoked in both neurons by a mechanical, but not by an electrical, stimulus to the mechanoreceptor in the shaft of a seta at the opposite side of an epidermis-muscle-nerve-cord preparation. This response was correlated to 84% of the amplitude, 73% of the rising rate and 81% of the duration of a longitudinal muscle contraction recorded by a mechano-electrical transducer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Earthworm muscle contraction; Main generator source; M-1 and M-2 neurons; Mechanical stimulus.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000009

Quantitative receptor radioautography in the study of receptor-receptor interactions in the nucleus tractus solitarii
Provedor de dados: 56
Autores: Fior-Chadi,D.R.; Fuxe,K.
The nucleus tractus solitarii (NTS) in the dorsomedial medulla comprises a wide range of neuropeptides and biogenic amines. Several of them are related to mechanisms of central blood pressure control. Angiotensin II (Ang II), neuropeptide Y (NPY) and noradrenaline (NA) are found in the NTS cells, as well as their receptors. Based on this observation we have evaluated the modulatory effect of these peptide receptors on &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;2-adrenoceptors in the NTS. Using quantitative receptor radioautography, we observed that NPY and Ang II receptors decreased the affinity of &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;2-adrenoceptors for their agonists in the NTS of the rat. Cardiovascular experiments agreed with the in vitro data....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quantitative radioautography; Nucleus tractus solitarii; Alpha-2-adrenoceptors; Neuropeptide Y receptors; Angiotensin II receptors.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200006

Effect of neoadjuvant chemotherapy on low-density lipoprotein (LDL) receptor and LDL receptor-related protein 1 (LRP-1) receptor in locally advanced breast cancer
Provedor de dados: 56
Autores: Pires,L.A.; Hegg,R.; Freitas,F.R.; Tavares,E.R.; Almeida,C.P.; Baracat,E.C.; Maranhão,R.C.
Low-density lipoprotein (LDL) receptors are overexpressed in most neoplastic cell lines and provide a mechanism for the internalization and concentration of drug-laden nanoemulsions that bind to these receptors. The aim of the present study was to determine whether the administration of standard chemotherapeutic schemes can alter the expression of LDL and LDL receptor-related protein 1 (LRP-1) receptors in breast carcinoma. Fragments of tumoral and normal breast tissue from 16 consecutive volunteer women with breast cancer in stage II or III were obtained from biopsies before the beginning of neoadjuvant chemotherapy and after chemotherapy, from fragments excised during mastectomy. Tissues were analyzed by immunohistochemistry for both receptors. Because...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Lipoproteins; LDL receptor; LRP-1 receptor; Chemotherapy; Nanoemulsion; Drug delivery.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600012

N-terminal amino acids of bovine alpha interferons are relevant for the neutralization of their antiviral activity
Provedor de dados: 56
Autores: Barreto Filho,J.B.; Eiras,P.R.S.; Golgher,R.R.
The structure-function relationship of interferons (IFNs) has been studied by epitope mapping. Epitopes of bovine IFNs, however, are practically unknown, despite their importance in virus infections and in the maternal recognition of pregnancy. It has been shown that recombinant bovine (rBo)IFN-alphaC and rBoIFN-alpha1 differ only in 12 amino acids and that the F12 monoclonal antibody (mAb) binds to a linear sequence of residues 10 to 34. We show here that the antiviral activities of these two IFNs were neutralized by the F12 mAb to different extents using two tests. In residual activity tests the antiviral activity dropped by more than 99% with rBoIFN-alphaC and by 84% with rBoIFN-alpha1. In checkerboard antibody titrations, the F12 mAb titer was 12,000...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bovine alpha interferon; Neutralization; Amino acid; Epitope.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500015

Prognostic factors in patients undergoing early-start peritoneal dialysis within 24 h after catheter insertion
Provedor de dados: 56
Autores: Jiang,Hong Ying; Huang,Dan Ju; Bai,Yi Hua; Li,Ji Sai; Pi,Hong Yan; Chen,Jing; Li,Luo Hua; Li,Jing.
This study aimed to investigate the clinical characteristics, prognosis, and factors for survival of patients who underwent early-start peritoneal dialysis (PD) within 24 h after catheter insertion three years after PD. This study was conducted from January 1, 2013 to December 31, 2017. All adult patients who were diagnosed with end-stage renal disease (ESRD) and underwent PD for the first time within 24 h after catheter insertion in our hospital were included. All patients with PD were followed-up until they withdrew from PD, switching to hemodialysis, were transferred to other medical centers, underwent renal transplantation, died or were lost to follow-up, or continued to undergo dialysis until the end of the study period. The follow-up observation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peritoneal dialysis; Early-start peritoneal dialysis; Survival rates; End stage renal disease; Dialysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300608

Twenty-four-hour esophageal pH monitoring in children and adolescents with chronic and/or recurrent rhinosinusitis
Provedor de dados: 56
Autores: Monteiro,V.R.S.G.; Sdepanian,V.L.; Weckx,L.; Fagundes-Neto,U.; Morais,M.B.
Gastroesophageal reflux (GER) disorder was studied in children and adolescents with chronic and/or recurrent rhinosinusitis not associated with bronchial asthma. Ten children with a clinical and radiological diagnosis of chronic and/or recurrent rhinosinusitis, consecutively attended at the Pediatric Otolaryngology Outpatient Clinic, Federal University of São Paulo, were evaluated. Prolonged esophageal pH monitoring was used to investigate GER disorder. The mean age of the ten patients evaluated (eight males) was 7.4 ± 2.4 years. Two patients presented vomiting as a clinical manifestation and one patient presented retrosternal pain with a burning sensation. Twenty-four-hour esophageal pH monitoring was performed using the Sandhill apparatus. An antimony...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sinusitis; Chronic disease; Gastroesophageal reflux; Children; Adolescents; Esophageal pH monitoring.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200009

The powerful high pressure tool for protein conformational studies
Provedor de dados: 56
Autores: Marchal,S.; Torrent,J.; Masson,P.; Kornblatt,J.M.; Tortora,P.; Fusi,P.; Lange,R.; Balny,C.
The pressure behavior of proteins may be summarized as a the pressure-induced disordering of their structures. This thermodynamic parameter has effects on proteins that are similar but not identical to those induced by temperature, the other thermodynamic parameter. Of particular importance are the intermolecular interactions that follow partial protein unfolding and that give rise to the formation of fibrils. Because some proteins do not form fibrils under pressure, these observations can be related to the shape of the stability diagram. Weak interactions which are differently affected by hydrostatic pressure or temperature play a determinant role in protein stability. Pressure acts on the 2º, 3º and 4º structures of proteins which are maintained by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High pressure; Prion protein; Amyloid; Ataxin; Enolase; Butyrylcholinesterase.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800004

Serial blood pressure measurements
Provedor de dados: 56
Autores: Koehler,N.R.; Figueiredo,C.E.P.; Ribeiro,A.C.M.
The objective of the present investigation was to study the effects of a 60-s interval of venous congestion between two noninvasive measurements of arterial blood pressure (ABP) on the fluctuation of ABP, assessed by the standard deviation of the differences between two readings. ABP was measured in 345 successive patients, at rest, four times each. For 269 participants, one pair of readings was obtained with a 60-s interval and the other pair without an interval. For 76 patients, the first pair was read at the same interval, and the second pair had venous congestion interposed and there was no waiting interval. There was no increased ABP oscillation, either when there was no interval between ABP readings, or when venous congestion was interposed compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood pressure; Arterial blood pressure oscillations; Venous congestion; Arterial blood pressure measurement technique; Arterial blood pressure fluctuations in the short term.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500007

Clinical efficacy of tirofiban combined with a Solitaire stent in treating acute ischemic stroke
Provedor de dados: 56
Autores: Zhang,Yang; Zhang,Qing-Qing; Fu,Cong; Wang,Lei; Zhang,Guan-Qun; Cao,Pei-Wei; Chen,Guo-Fang; Fu,Xin-Min.
This study explores the safety and effect of acute cerebral infarction treatment by microcatheter injection of tirofiban combined with a Solitaire AB stent and/or stent implantation. Emergency cerebral angiograms showing the responsible vascular occlusion of 120 acute cerebral infarction patients who underwent emergency endovascular thrombectomy were included in the study. These patients were randomly divided into two groups using the random number table method: treatment group (n=60) that received thrombectomy (with cerebral artery stents) combined with intracerebral injection of tirofiban and control group (n=60) that only received thrombectomy (with cerebral artery stents alone). The baseline data, cerebral angiography before and after surgery,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute ischemic stroke; Solitaire AB stent; Endovascular thrombectomy; Tirofiban.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000603

The maternal JAK/STAT pathway of Drosophila regulates embryonic dorsal-ventral patterning
Provedor de dados: 56
Autores: Lopes,E.S.S.; Araujo,H.M.
Activation of NFkappaB plays a pivotal role in many cellular processes such as inflammation, proliferation and apoptosis. In Drosophila, nuclear translocation of the NFkappaB-related transcription factor Dorsal is spatially regulated in order to subdivide the embryo into three primary dorsal-ventral (DV) domains: the ventral presumptive mesoderm, the lateral neuroectoderm and the dorsal ectoderm. Ventral activation of the Toll receptor induces degradation of the IkappaB-related inhibitor Cactus, liberating Dorsal for nuclear translocation. In addition, other pathways have been suggested to regulate Dorsal. Signaling through the maternal BMP member Decapentaplegic (Dpp) inhibits Dorsal translocation along a pathway parallel to and independent of Toll. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: JAK kinase; Drosophila; Embryonic patterning; Hopscotch; NFkappaB.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200006

A high-carbohydrate diet enhances the adverse effect of the S2 allele of APOC3 SstI polymorphism on the TG/HDL-C ratio only in young Chinese females
Provedor de dados: 56
Autores: Song,Yong Yan; Gong,Ren Rong; Zhang,Zhen; Li,Yuan Hao; Xiao,Li Ying; Zhou,Xue Dong; Fang,Ding Zhi.
Both genetic background and diet have profound effects on plasma lipid profiles. We hypothesized that a high-carbohydrate (high-CHO) diet may affect the ratios of serum lipids and apolipoproteins (apo) differently in subjects with different genotypes of the SstI polymorphism in the apoCIII gene (APOC3). Fifty-six healthy university students (27 males and 29 females, 22.89 ± 1.80 years) were given a washout diet of 54% carbohydrate for 7 days, followed by a high-CHO diet of 70% carbohydrate for 6 days without total energy restriction. Serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apoB100, apoAI, and the APOC3 SstI polymorphism were analyzed. The ratios of serum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High-carbohydrate diet; Lipid ratio; Gene polymorphism; Human APOC3.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600005

Does acute hyperglycemia alter rat aortic depressor nerve function?
Provedor de dados: 56
Autores: Huber,D.A.; Carmo,J.M. do; Castania,J.A.; Fazan Jr,R.; Salgado,H.C.
Because it is not known where in the reflex arch, i.e., afference, central nervous system or efferences, hyperglycemia affects baroreflex function, the present study examined the effect of short-term (30 min) hyperglycemia on aortic depressor nerve function measured by a mean arterial pressure vs aortic depressor nerve activity curve, fitted by sigmoidal regression, or by cross-spectral analysis between mean arterial pressure and aortic depressor nerve activity. Anesthetized male Wistar rats received an intravenous bolus (0.25 mL) injection, followed by 30 min of infusion (1 mL/h) of 30% glucose (N = 14). Control groups received a bolus injection and infusion of 0.9% saline (N = 14), or 30% mannitol (N = 14). Glucose significantly increased both blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pressoreceptors; Hyperglycemia; Arterial pressure; Spectral analysis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100017

Comparative effects of organic and inorganic mercury on in vivo dopamine release in freely moving rats
Provedor de dados: 56
Autores: Faro,L.R.F.; Rodrigues,K.J.A.; Santana,M.B.; Vidal,L.; Alfonso,M.; Durán,R.
The present study was carried out in order to compare the effects of administration of organic (methylmercury, MeHg) and inorganic (mercury chloride, HgCl 2 ) forms of mercury on in vivo dopamine (DA) release from rat striatum. Experiments were performed in conscious and freely moving female adult Sprague-Dawley (230-280 g) rats using brain microdialysis coupled to HPLC with electrochemical detection. Perfusion of different concentrations of MeHg or HgCl 2 (2 µL/min for 1 h, N = 5-7/group) into the striatum produced significant increases in the levels of DA. Infusion of 40 µM, 400 µM, or 4 mM MeHg increased DA levels to 907 ± 31, 2324 ± 156, and 9032 ± 70% of basal levels, respectively. The same concentrations of HgCl 2 increased DA levels to 1240 ± 66,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methylmercury; Mercury chloride; Dopamine release in vivo; Microdialysis; Mercury determination; Striatum.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000009

Tacrolimus (FK506) reduces ischemia-induced hippocampal damage in rats: a 7- and 30-day study
Provedor de dados: 56
Autores: Giordani,F.; Benetolli,A.; Favero-Filho,L.A.; Lima,K.C.M.; Cestari Junior,L.; Milani,H.
The neuroprotective effect of the immunosuppressant agent FK506 was evaluated in rats after brain ischemia induced for 15 min in the 4-vessel occlusion model. In the first experimental series, single doses of 1.0, 3.0 or 6.0 mg FK506/kg were given intravenously (iv) immediately after ischemia. In the second series, FK506 (1.0 mg/kg) was given iv at the beginning of reperfusion, followed by doses applied intraperitoneally (ip) 6, 24, 48, and 72 h post-ischemia. The same protocol was used in the third series except that all 5 doses were given iv. Damage to the hippocampal field CA1 was assessed 7 or 30 days post-ischemia on three different stereotaxic planes along the septotemporal axis of the hippocampus. Ischemia caused marked neurodegeneration on all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebral ischemia; Hippocampal cell death; FK506; Sustained neuroprotection.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400012

Solubilization of human erythrocyte membranes by ASB detergents
Provedor de dados: 56
Autores: Domingues,C.C.; Malheiros,S.V.P.; de Paula,E.
Understanding the membrane solubilization process and finding effective solubilizing agents are crucial challenges in biochemical research. Here we report results on the interaction of the novel linear alkylamido propyl dimethyl amino propanosulfonate detergents, ASB-14 and ASB-16, with human erythrocyte membranes. An estimation of the critical micelle concentration of these zwitterionic detergents (ASB-14 = 100 µM and ASB-16 = 10 µM) was obtained using electron paramagnetic resonance. The amount of proteins and cholesterol solubilized from erythrocytes by these detergents was then determined. The hemolytic activities of the ASB detergents were assayed and the detergent/lipid molar ratios for the onset of hemolysis (Re sat) and total lysis (Re sol) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemolysis; Protein solubilization; Erythrocyte; Zwitterionic detergents; Critical micelle concentration; Cholesterol solubilization.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900003

Astragalus polysaccharides decrease proliferation, migration, and invasion but increase apoptosis of human osteosarcoma cells by up-regulation of microRNA-133a
Provedor de dados: 56
Autores: Chu,Yanchen; Fang,Yuan; Chi,Jingwei; Li,Jing; Zhang,Dongyang; Zou,Yunwen; Wang,Zhijie.
Osteosarcoma (OS) has a high incidence, malignity, and frequency of recurrence and metastasis. In this study, we aimed to explore the potential anti-cancer effects of Astragalus polysaccharides (APS) on human OS MG63 cells as well as underlying mechanisms. Viability of MG63 cells was assessed by CCK-8 assay to determine the adequate concentration of APS. Then, effects of APS on MG63 cell proliferation, cell cycle distribution, apoptosis, and migration and invasion were analyzed by BrdU incorporation, PI staining, flow cytometry, and transwell assays, respectively. The expression levels of proteins involved in these physiological processes were assessed by western blot analysis. Afterwards, miR-133a level in APS-treated cells was determined by qRT-PCR, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteosarcoma; Astragalus polysaccharides; Anti-tumor; MicroRNA-133a; JNK.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200610

Sedative and anesthetic activities of the essential oils of Hyptis mutabilis (Rich.) Briq. and their isolated components in silver catfish (Rhamdia quelen)
Provedor de dados: 56
Autores: Silva,L.L.; Garlet,Q.I.; Benovit,S.C.; Dolci,G.; Mallmann,C.A.; Bürger,M.E.; Baldisserotto,B.; Longhi,S.J.; Heinzmann,B.M.
This study evaluated the sedative and anesthetic effects of the essential oils (EO) of Hyptis mutabilis (Rich.) Briq. and their isolated components on silver catfish (Rhamdia quelen). Quantitative chemical differences between the EOs obtained from leaves and inflorescences were verified, and a new chemotype rich in globulol was described. Although there were no significant differences in the time of induction for sedation and anesthesia between the EOs, only the leaf EO at 344 mg/L anesthetized all fish without side effects. Fractionation of the leaf EO was carried out by column chromatography. The isolated compounds [(+)-1-terpinen-4-ol and (-)-globulol] showed different activity from that detected for the leaf EO in proportional concentrations and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silver catfish; (+)-1-Terpinen-4-ol; (-)-Globulol; Sedation; GABA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900771

Non-invasive messenger RNA transcriptional evaluation in human kidney allograft dysfunction
Provedor de dados: 56
Autores: Joelsons,G.; Domenico,T.; Gonçalves,L.F.; Manfro,R.C.
The aim of the present study was to evaluate messenger RNA expression in kidney allograft recipients. Forty-four kidney transplant recipients were evaluated up to three months after grafting. After transplantation, peripheral blood samples were drawn sequentially for real-time polymerase chain reaction analyses of perforin and TIM-3 genes. Biopsies were obtained to evaluate acute graft dysfunction and interpreted according to the Banff classification. Eight patients presented episodes of acute rejection. Recipients with rejection had significantly higher levels of TIM-3 mRNA transcripts compared to those without rejection (median gene expression 191.2 and 36.9 mRNA relative units, respectively; P&lt;0.0001). Also, perforin gene expression was higher in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney transplantation; Acute rejection; Gene expression; Diagnosis; MRNA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700608

Salivary cortisol and α-amylase: subclinical indicators of stress as cardiometabolic risk
Provedor de dados: 56
Autores: Cozma,S.; Dima-Cozma,L.C.; Ghiciuc,C.M.; Pasquali,V.; Saponaro,A.; Patacchioli,F.R.
Currently, the potential for cardiovascular (CV) stress-induced risk is primarily based on the theoretical (obvious) side effects of stress on the CV system. Salivary cortisol and α-amylase, produced respectively by the hypothalamus-pituitary-adrenal (HPA) axis and the sympathetic-adrenomedullary (SAM) system during stress response, are still not included in the routine evaluation of CV risk and require additional and definitive validation. Therefore, this article overviews studies published between 2010 and 2015, in which salivary cortisol and α-amylase were measured as stress biomarkers to examine their associations with CV/CMR (cardiometabolic risk) clinical and subclinical indicators. A comprehensive search of PubMed, Web of Science and Scopus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salivary stress biomarkers; Cardiometabolic risk (factors); Stress; Hypothalamic-pituitary-adrenal axis; Sympathetic adrenomedullary system.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200201

MHC-restricted antigen presentation and recognition: constraints on gene, recombinant and peptide vaccines in humans
Provedor de dados: 56
Autores: Cunha-Neto,E.
The target of any immunization is to activate and expand lymphocyte clones with the desired recognition specificity and the necessary effector functions. In gene, recombinant and peptide vaccines, the immunogen is a single protein or a small assembly of epitopes from antigenic proteins. Since most immune responses against protein and peptide antigens are T-cell dependent, the molecular target of such vaccines is to generate at least 50-100 complexes between MHC molecule and the antigenic peptide per antigen-presenting cell, sensitizing a T cell population of appropriate clonal size and effector characteristics. Thus, the immunobiology of antigen recognition by T cells must be taken into account when designing new generation peptide- or gene-based vaccines....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaccines; MHC; Antigen recognition; Antigen processing; T cells; Molecular evolution.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200008

Clinical utility of standard base excess in the diagnosis and interpretation of metabolic acidosis in critically ill patients
Provedor de dados: 56
Autores: Park,M; Taniguchi,L.U; Noritomi,D.T; Libório,A.B.; Maciel,A.T; Cruz-Neto,L.M.
The aims of this study were to determine whether standard base excess (SBE) is a useful diagnostic tool for metabolic acidosis, whether metabolic acidosis is clinically relevant in daily evaluation of critically ill patients, and to identify the most robust acid-base determinants of SBE. Thirty-one critically ill patients were enrolled. Arterial blood samples were drawn at admission and 24 h later. SBE, as calculated by Van Slyke's (SBE VS) or Wooten's (SBE W) equations, accurately diagnosed metabolic acidosis (AUC = 0.867, 95%CI = 0.690-1.043 and AUC = 0.817, 95%CI = 0.634-0.999, respectively). SBE VS was weakly correlated with total SOFA (r = -0.454, P &lt; 0.001) and was similar to SBE W (r = -0.482, P &lt; 0.001). All acid-base variables were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic acidosis; Clinical outcome; Strong ion gap; Standard base excess; Van Slyke equation; Sequential organ failure assessment.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300011

Autonomic nervous system dysfunction in children with severe tetanus: dissociation of cardiac and vascular sympathetic control
Provedor de dados: 56
Autores: Mazzei de Davila,C.A.; Davila,D.F.; Donis,J.H.; Gonzalo,X.
The medical records of ten pediatric patients with a clinical diagnosis of tetanus were reviewed retrospectively. The heart rate and blood pressure of all tetanus patients were measured noninvasively every hour during the first two weeks of hospitalization. Six of ten tetanus patients presented clinical evidence of sympathetic hyperactivity (group A) and were compared with a control group consisting of four children who required mechanical ventilation for diseases other than tetanus (group B). Heart rate and blood pressure simultaneously and progressively increased to a maximum by day 7. The increase over baseline was 43.70 ± 11.77 bpm (mean ± SD) for heart rate (P&lt;0.01) and 38.60 ± 26.40 mmHg for blood pressure (P&lt;0.01). These values were higher and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tetanus patients; Sympathetic control; Autonomic dysfunction; Blood pressure; Heart rate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600018

Chemoreceptors and cardiovascular control in acute and chronic systemic hypoxia
Provedor de dados: 56
Autores: Marshall,J.M.
This review describes the ways in which the primary bradycardia and peripheral vasoconstriction evoked by selective stimulation of peripheral chemoreceptors can be modified by the secondary effects of a chemoreceptor-induced increase in ventilation. The evidence that strong stimulation of peripheral chemoreceptors can evoke the behavioural and cardiovascular components of the alerting or defence response which is characteristically evoked by novel or noxious stimuli is considered. The functional significance of all these influences in systemic hypoxia is then discussed with emphasis on the fact that these reflex changes can be overcome by the local effects of hypoxia: central neural hypoxia depresses ventilation, hypoxia acting on the heart causes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypoxia; Adenosine; Chemoreceptors; Vasodilatation; Noradrenaline.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700002

Increased levels of glutamate in the central nervous system are associated with behavioral symptoms in experimental malaria
Provedor de dados: 56
Autores: Miranda,A.S.; Vieira,L.B.; Lacerda-Queiroz,N.; Souza,A.H.; Rodrigues,D.H.; Vilela,M.C.; Gomez,M.V.; Machado,F.S.; Rachid,M.A.; Teixeira,A.L.
Cerebral malaria (CM) is a severe complication resulting from Plasmodium falciparum infection. This condition has been associated with cognitive, behavioral and motor dysfunctions, seizures and coma. The underlying mechanisms of CM are incompletely understood. Glutamate and other metabolites such as lactate have been implicated in its pathogenesis. In the present study, we investigated the involvement of glutamate in the behavioral symptoms of CM. Seventeen female C57BL/6 mice (20-25 g) aged 6-8 weeks were infected with P. berghei ANKA by the intraperitoneal route using a standardized inoculation of 10(6) parasitized red blood cells suspended in 0.2 mL PBS. Control animals (N = 17) received the same volume of PBS. Behavioral and neurological symptoms were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cerebral malaria; Glutamate; Cerebrospinal fluid; Behavioral changes; SHIRPA.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200006

Pressure and time dependence of the cardiopulmonary reflex response in patients with hypertensive cardiomyopathy
Provedor de dados: 56
Autores: Otto,M.E.B.; Consolim-Colombo,F.M.; Rodrigues Sobrinho,C.R.M.; Krieger,E.M.
The first minutes of the time course of cardiopulmonary reflex control evoked by lower body negative pressure (LBNP) in patients with hypertensive cardiomyopathy have not been investigated in detail. We studied 15 hypertensive patients with left ventricular dysfunction (LVD) and 15 matched normal controls to observe the time course response of the forearm vascular resistance (FVR) during 3 min of LBNP at -10, -15, and -40 mmHg in unloading the cardiopulmonary receptors. Analysis of the average of 3-min intervals of FVR showed a blunted response of the LVD patients at -10 mmHg (P = 0.03), but a similar response in both groups at -15 and -40 mmHg. However, using a minute-to-minute analysis of the FVR at -15 and -40 mmHg, we observed a similar response in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baroreflex; Essential hypertension; Heart failure; Cardiopulmonary reflex; Baroreceptors.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100004

Functional changes of human quadriceps muscle injured by eccentric exercise
Provedor de dados: 56
Autores: Serrão,F.V.; Foerster,B.; Spada,S.; Morales,M.M.B.; Monteiro-Pedro,V.; Tannús,A.; Salvini,T.F.
The present study evaluated functional changes of quadriceps muscle after injury induced by eccentric exercise. Maximal isometric torque of quadriceps and the surface electromyography (root mean square, RMS, and median frequency, MDF) of the vastus medialis oblique (VMO) and vastus lateralis (VL) muscles were examined before, immediately after and during the first 7 days after injury. Serum creatine kinase (CK) levels and magnetic resonance imaging (MRI) were used to identify muscle injury. The subject was used as her own control and percent refers to pre-injury data. Experiments were carried out with a sedentary 23-year-old female. Injury was induced by 4 bouts of 15 maximal isokinetic eccentric contractions (angular velocity of 5º/s; range of motion from...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Isometric torque; Surface electromyography; Quadriceps muscle; Muscle injury; Eccentric exercise; Magnetic resonance imaging.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600014

Influence of progesterone on GAD65 and GAD67 mRNA expression in the dorsolateral striatum and prefrontal cortex of female rats repeatedly treated with cocaine
Provedor de dados: 56
Autores: Souza,M.F.; Toniazo,V.M.; Frazzon,A.P.G.; Barros,H.M.T.
Female rats are intensely affected by cocaine, with estrogen probably playing an important role in this effect. Progesterone modulates the GABA system and attenuates the effects of cocaine; however, there is no information about its relevance in changing GABA synthesis pathways after cocaine administration to female rats. Our objective was to investigate the influence of progesterone on the effects of repeated cocaine administration on the isoenzymes of glutamic acid decarboxylase (GAD65 and GAD67) mRNA in brain areas involved in the addiction circuitry. Ovariectomized, intact and progesterone replacement-treated female rats received saline or cocaine (30 mg/kg, ip) acutely or repeatedly. GAD isoenzyme mRNA levels were determined in the dorsolateral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocaine; Progesterone; GAD mRNA; Dorsolateral striatum; Prefrontal cortex.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100011

Excitotoxic median raphe lesions aggravate working memory storage performance deficits caused by scopolamine infusion into the dentate gyrus of the hippocampus in the inhibitory avoidance task in rats
Provedor de dados: 56
Autores: Babar,E.; Melik,E.; Özgünen,T.
The interactions between the median raphe nucleus (MRN) serotonergic system and the septohippocampal muscarinic cholinergic system in the modulation of immediate working memory storage performance were investigated. Rats with sham or ibotenic acid lesions of the MRN were bilaterally implanted with cannulae in the dentate gyrus of the hippocampus and tested in a light/dark step-through inhibitory avoidance task in which response latency to enter the dark compartment immediately after the shock served as a measure of immediate working memory storage. MRN lesion per se did not alter response latency. Post-training intrahippocampal scopolamine infusion (2 and 4 µg/side) produced a more marked reduction in response latencies in the lesioned animals compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Working memory; Median raphe nucleus; Serotonin; Scopolamine; Acetylcholine; Inhibitory avoidance.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400012

Effects of elevated calcium on motor and exploratory activities of rats
Provedor de dados: 56
Autores: Godinho,A.F.; Trombini,T.V.; Oliveira,E.C.
The effects of serum and brain calcium concentration on rat behavior were tested by maintaining animals on either distilled water (N = 60) or water containing 1% calcium gluconate (N = 60) for 3 days. Animals that were maintained on high calcium drinking water presented increased serum calcium levels (control = 10.12 ± 0.46 vs calcium treated = 11.62 ± 0.51 µg/dl). Increase of brain calcium levels was not statistically significant. In the behavioral experiments each rat was used for only one test. Rats that were maintained on high calcium drinking water showed increased open-field behavior of ambulation (20.68%) and rearing (64.57%). On the hole-board, calcium-supplemented animals showed increased head-dip (67%) and head-dipping (126%), suggesting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium supplementation; Behavior; Rat; Motor activity; Exploratory activity.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400007

Neonatal administration of citalopram delays somatic maturation in rats
Provedor de dados: 56
Autores: Deiró,T.C.B.J.; Manhães-de-Castro,R.; Cabral-Filho,J.E.; Souza,S.L.; Freitas-Silva,S.R.; Ferreira,L.M.P.; Guedes,R.C.A.; Câmara,C.R.V.; Barros,K.M.F.T.
We investigated the somatic maturation of neonate rats treated during the suckling period with citalopram, a selective serotonin reuptake inhibitor. Groups with 6 male neonates were randomly assigned to different treatments 24 h after birth. Each litter was suckled by one of the dams until the 21st postnatal day. Body weight, head axis and tail length were measured daily from the 1st to the 21st postnatal day. Time of ear unfolding, auditory conduit opening, incisor eruption, and eye opening was determined. Pups received 5 mg (Cit5), 10 mg (Cit10) or 20 mg/kg (Cit20) citalopram sc, or saline (0.9% NaCl, w/v, sc). Compared to saline, body weight was lower (24.04%, P &lt; 0.01) for Cit10 from the 10th to the 21st day and for Cit20 from the 6th to the 21st...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serotonin; Growth indicators; Selective serotonin reuptake inhibitor; Citalopram; Craniofacial; Skull.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000009

Taurine inhibits serum deprivation-induced osteoblast apoptosis via the taurine transporter/ERK signaling pathway
Provedor de dados: 56
Autores: Zhang,Lei-Yi; Zhou,Yue-Ying; Chen,Fei; Wang,Bing; Li,Jing; Deng,You-Wen; Liu,Wei-Dong; Wang,Zheng-Guang; Li,Ya-Wei; Li,Dong-Zhe; Lv,Guo-Hua; Yin,Bang-Liang.
Taurine has positive effects on bone metabolism. However, the effects of taurine on osteoblast apoptosis in vitro have not been reported. The aim of this study was to investigate the activity of taurine on apoptosis of mouse osteoblastic MC3T3-E1 cells. The data showed that 1, 5, 10, or 20 mM taurine resulted in 16.7, 34.2, 66.9, or 63.75% reduction of MC3T3-E1 cell apoptosis induced by the serum deprivation (serum-free α-MEM), respectively. Taurine (1, 5, or 10 mM) also reduced cytochrome c release and inhibited activation of caspase-3 and -9, which were measured using fluorogenic substrates for caspase-3/caspase-9, in serum-deprived MC3T3-E1 cells. Furthermore, taurine (10 mM) induced extracellular signal-regulated kinase (ERK) phosphorylation in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Taurine; Osteoblasts; Apoptosis; Taurine transporter; ERK.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700002

Desmin: molecular interactions and putative functions of the muscle intermediate filament protein
Provedor de dados: 56
Autores: Costa,M.L.; Escaleira,R.; Cataldo,A.; Oliveira,F.; Mermelstein,C.S.
Desmin is the intermediate filament (IF) protein occurring exclusively in muscle and endothelial cells. There are other IF proteins in muscle such as nestin, peripherin, and vimentin, besides the ubiquitous lamins, but they are not unique to muscle. Desmin was purified in 1977, the desmin gene was characterized in 1989, and knock-out animals were generated in 1996. Several isoforms have been described. Desmin IFs are present throughout smooth, cardiac and skeletal muscle cells, but can be more concentrated in some particular structures, such as dense bodies, around the nuclei, around the Z-line or in costameres. Desmin is up-regulated in muscle-derived cellular adaptations, including conductive fibers in the heart, electric organs, some myopathies, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Desmin; Intermediate filaments; Muscle; Myogenesis; Myopathy.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200007

Disaccharidase levels in normal epithelium of the small intestine of rats with iron-deficiency anemia
Provedor de dados: 56
Iron-deficiency anemia is the nutritional deficiency most frequently occurring throughout the world, which manifests as a complex systemic disease involving all cells, affecting enzyme activities and modifying protein synthesis. In view of these considerations, the objective of the present study was to determine the effects of iron-deficiency anemia on disaccharidases and on the epithelial morphokinetics of the jejunal mucosa. Newly weaned male Wistar rats were divided into 4 groups of 10 animals each: C6w received a standard ration containing 36 mg elemental iron per kg ration for 6 weeks; E6w received an iron-poor ration (5-8 mg/kg ration) for 6 weeks; C10w received an iron-rich ration (36 mg/kg ration) for 10 weeks; E10w received an iron-poor ration for...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Disaccharidases; Anemia; Iron deficiency; Intestinal mucosa.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700005

Effects of lipopolysaccharide on low- and high-density cultured rabbit vascular smooth muscle cells: differential modulation of nitric oxide release, ERK1/ERK2 MAP kinase activity, protein tyrosine phosphatase activity, and DNA synthesis
Provedor de dados: 56
Autores: Barbosa de Oliveira,L.C.; Rocha Oliveira,C.J.; Fries,D.M.; Stern,A.; Monteiro,H.P.
Previous studies have shown that exogenously generated nitric oxide (NO) inhibits smooth muscle cell proliferation. In the present study, we stimulated rabbit vascular smooth muscle cells (RVSMC) with E. coli lipopolysaccharide (LPS), a known inducer of NO synthase transcription, and established a connection between endogenous NO, phosphorylation/dephosphorylation-mediated signaling pathways, and DNA synthesis. Non-confluent RVSMC were cultured with 0, 5, 10, or 100 ng/ml of the endotoxin. NO release was increased by 86.6% (maximum effect) in low-density cell cultures stimulated with 10 ng/ml LPS as compared to non-stimulated controls. Conversely, LPS (5 to 100 ng/ml) did not lead to enhanced NO production in multilayered (high density) RVSMC. DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Lipopolysaccharide; Protein tyrosine phosphatases; Mitogen-activated protein kinases; DNA synthesis; Cell density.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200006

Synergistic control of forearm based on accelerometer data and artificial neural networks
Provedor de dados: 56
Autores: Mijovic,B.; Popovic,M.B.; Popovic,D.B.
In the present study, we modeled a reaching task as a two-link mechanism. The upper arm and forearm motion trajectories during vertical arm movements were estimated from the measured angular accelerations with dual-axis accelerometers. A data set of reaching synergies from able-bodied individuals was used to train a radial basis function artificial neural network with upper arm/forearm tangential angular accelerations. The trained radial basis function artificial neural network for the specific movements predicted forearm motion from new upper arm trajectories with high correlation (mean, 0.9149-0.941). For all other movements, prediction was low (range, 0.0316-0.8302). Results suggest that the proposed algorithm is successful in generalization over...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Synergy; Accelerometers; Artificial neural network; Control; Neural prosthesis; Functional electrical stimulation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500007

Insulin impairs the maturation of chondrocytes in vitro
Provedor de dados: 56
Autores: Torres,E.S.; Andrade,C.V.; Fonseca,E.C.; Mello,M.A.; Duarte,M.E.L.
The precise nature of hormones and growth factors directly responsible for cartilage maturation is still largely unclear. Since longitudinal bone growth occurs through endochondral bone formation, excess or deficiency of most hormones and growth factors strongly influences final adult height. The structure and composition of the cartilaginous extracellular matrix have a critical role in regulating the behavior of growth plate chondrocytes. Therefore, the maintenance of the three-dimensional cell-matrix interaction is necessary to study the influence of individual signaling molecules on chondrogenesis, cartilage maturation and calcification. To investigate the effects of insulin on both proliferation and induction of hypertrophy in chondrocytes in vitro we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chondrocytes; Insulin; Micromass cultures; Apoptosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900007

Neuroethologic differences in sleep deprivation induced by the single- and multiple-platform methods
Provedor de dados: 56
Autores: Medeiros,R.; Lenneberg-Hoshino,C.; Hoshino,K.; Tufik,S.
It has been proposed that the multiple-platform method (MP) for desynchronized sleep (DS) deprivation eliminates the stress induced by social isolation and by the restriction of locomotion in the single-platform (SP) method. MP, however, induces a higher increase in plasma corticosterone and ACTH levels than SP. Since deprivation is of heuristic value to identify the functional role of this state of sleep, the objective of the present study was to determine the behavioral differences exhibited by rats during sleep deprivation induced by these two methods. All behavioral patterns exhibited by a group of 7 albino male Wistar rats submitted to 4 days of sleep deprivation by the MP method (15 platforms, spaced 150 mm apart) and by 7 other rats submitted to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sleep deprivation methods; Stress; Social interactions; Group crowding effects.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500012

Identifying reasons for failure in biomedical research and publishing
Provedor de dados: 56
Autores: Bousfield,D.
The regular assessment of Brazilian scientific output means that individual university departments need to constantly improve the quantity and quality of their scientific output. A significant proportion of this output involves the work of Master’s and Doctoral students, but getting this work published in a suitable journal can often prove to be a challenge. Although students’ lack of fluency in English is a contributing factor, many of the problems observed have an early origin in the formulation of the research problem and its relevance to current research trends in the international literature. In short, more time needs to be spent in the library and less in the laboratory, and more effort needs to be made in teaching students basic research skills such...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Research failure; Reading; Scientific writing; Scientific literacy.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700001

Extracts of Ascaris suum egg and adult worm share similar immunosuppressive properties
Provedor de dados: 56
Autores: Souza,V.M.O.; Faquim-Mauro,E.L.; Macedo,M.S.
Adult Ascaris suum body extract (Asc) prepared from male and female worms (with stored eggs) down-regulates the specific immune response of DBA/2 mice to ovalbumin (OA) and preferentially stimulates a Th2 response to its own components, which is responsible for the suppression of the OA-specific Th1 response. Here, we investigated the participation of soluble extracts prepared from male or female worms or from eggs (E-Asc) in these immunological events. Extracts from either sex (1 mg/animal) or E-Asc (0.35 or 1 mg protein/animal) suppressed the delayed-type hypersensitivity (DTH) reaction (60-85%), proliferative response (50-70%), IL-2 and IFN-gamma secretion (below detection threshold) and IgG1 antibody production (70-90%) of DBA/2 mice to OA. A dose of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascaris suum; Immunosuppression; Adult worms; Eggs; Cytokines.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100012

Identification of rounded atelectasis in workers exposed to asbestos by contrast helical computed tomography
Provedor de dados: 56
Autores: Terra-Filho,M.; Kavakama,J.; Bagatin,E.; Capelozzi,V.L.; Nery,L.E.; Tavares,R.
Rounded atelectasis (RA) is a benign and unusual form of subpleural lung collapse that has been described mostly in asbestos-exposed workers. This form of atelectasis manifests as a lung nodule and can be confused with bronchogenic carcinoma upon conventional radiologic examination. The objective of the present study was to evaluate the variation in contrast uptake in computed tomography for the identification of asbestos-related RA in Brazil. Between January 1998 and December 2000, high-resolution computed tomography (HRCT) was performed in 1658 asbestos-exposed workers. The diagnosis was made in nine patients based on a history of prior asbestos exposure, the presence of characteristic (HRCT) findings and lesions unchanged in size over 2 years or more....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rounded atelectasis; Asbestos; Helical computed tomography; Asbestos-exposed workers.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000010

Rabies infection and specific effect of vaccination in mice selected for high and low immunobiological parameters
Provedor de dados: 56
Autores: Queiroz-da-Silva,L.H.; De-Franco,M.; Sant'Anna,O.A.
Innate and acquired resistance to rabies infection was investigated in mice genetically selected for high (H) or low (L) antibody responsiveness from selections I, III and IV and in mice selected for maximal (AIRmax) or minimal (AIRmin) acute inflammatory reaction. These mouse lines were infected intramuscularly with different virus dilutions and the LD50 was determined. The HIII and HIV mouse lines were more susceptible than the LIII and LIV lines and the HI line showed a discrete but higher resistance than the LI line. Analysis of the interline (H x L) F1 hybrids from selections III and IV indicated different dominance effects on the "resistant" and" susceptible" phenotypes when the route of vaccination was changed. No differences were observed between...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rabies; Antibody; Vaccination; High and low antibody responder mice.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100008

Effect of high hydrostatic pressure on the barrier properties of polyamide-6 films
Provedor de dados: 56
Autores: Schmerder,A.; Richter,T.; Langowski,H.-C.; Ludwig,H.
Little is known about the barrier properties of polymer films during high pressure processing of prepackaged foods. In order to learn more about this, we examined the influence of high hydrostatic pressure on the permeation of raspberry ketone (dissolved in ethanol/water) through polyamide-6 films at temperatures between 20 and 60ºC. Permeation was lowered by increasing pressure at all temperatures. At 23°C, the increasing pressure sequence 0.1, 50, 100, 150, and 200 MPa correlated with the decreasing permeation coefficients P/(10(9) cm² s-1) of 6.2, 3.8, 3.0, 2.2, and 1.6. Analysis of the permeation kinetics indicated that this effect was due to a reduced diffusion coefficient. Pressure and temperature acted antagonistically to each other. The decrease in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydrostatic pressure; Polyamide-6 films; Permeation through polymers; Raspberry ketone.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800018

Plasma von Willebrand factor as a predictor of survival in pulmonary arterial hypertension associated with congenital heart disease
Provedor de dados: 56
Autores: Lopes,A.A.; Barreto,A.C.; Maeda,N.Y.; Cícero,C.; Soares,R.P.S.; Bydlowski,S.P.; Rich,S.
Biomarkers have been identified for pulmonary arterial hypertension, but are less well defined for specific etiologies such as congenital heart disease-associated pulmonary arterial hypertension (CHDPAH). We measured plasma levels of eight microvascular dysfunction markers in CHDPAH, and tested for associations with survival. A cohort of 46 inoperable CHDPAH patients (age 15.0 to 60.2 years, median 33.5 years, female:male 29:17) was prospectively followed for 0.7 to 4.0 years (median 3.6 years). Plasma levels of von Willebrand factor antigen (VWF:Ag), tissue plasminogen activator (t-PA) and its inhibitor (PAI-1), P-selectin, reactive C-protein, tumor necrosis factor alpha, and interleukin-6 and -10 were measured at baseline, and at 30, 90, and 180 days in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulmonary hypertension; Congenital heart disease; Eisenmenger syndrome; Endothelial dysfunction; Von Willebrand factor; Th2 cytokine response.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200011

Reports of investigations using quantitative methods in the clinical setting: a call for papers
Provedor de dados: 56
Autores: Fuchs,F.D.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Clinical investigation; Quantitative methods; Clinical epidemiology.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100001

The elevated gradient of aversion: a new apparatus to study the rat behavior dimensions of anxiety, fear, and impulsivity
Provedor de dados: 56
Autores: Rico,J.L.; Bonuti,R.; Morato,S.
Few behavioral tests allow measuring several characteristics and most require training, complex analyses, and/or are time-consuming. We present an apparatus based on rat exploratory behavior. Composed of three different environments, it allows the assessment of more than one behavioral characteristic in a short 3-min session. Factorial analyses have defined three behavioral dimensions, which we named Exploration, Impulsivity, and Self-protection. Behaviors composing the Exploration factor were increased by chlordiazepoxide and apomorphine and decreased by pentylenetetrazole. Behaviors composing the Impulsivity factor were increased by chlordiazepoxide, apomorphine, and both acute and chronic imipramine treatments. Behaviors composing the Self-protection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anxiety; Exploratory behavior; Impulsivity; Rat; Fear; Self-protection.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100601

HtrA1 suppresses the growth of pancreatic cancer cells by modulating Notch-1 expression
Provedor de dados: 56
Autores: Cheng,Hao; Zhu,Hao; Cao,Meng; Lu,Chenglin; Bao,Shanhua; Pan,Yiming.
Pancreatic cancer is well known to be the most deadly malignancy with the worst survival rate of all cancers. High temperature requirement factor A1 (HtrA1) plays an important role in cancer cell proliferation, migration, apoptosis, and differentiation. This study aimed to explore the function of HtrA1 in pancreatic cancer cell growth and its underlying mechanism. We found that the expression of HtrA1 was lower in pancreatic cancer tissue compared to the adjacent normal tissue. Consistently, HtrA1 levels were also decreased in two human pancreatic cancer cell lines, PANC-1 and BXPC-3. Moreover, enforced expression of HtrA1 inhibited cell viability and colony formation of PANC-1 and BXPC-3 cells. Overexpression of HtrA1 promoted apoptosis and suppressed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pancreatic cancer; Proliferation; HtrA1; Notch-1; Molecular target.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100606

Regulation of T cell response to leishmania antigens by determinants of histocompatibility leukocyte class I and II molecules
Provedor de dados: 56
Autores: Bacellar,O.; Russo,C.; Carvalho,E.M.
It has been shown that HLA class I molecules play a significant role in the regulation of the proliferation of T cells activated by mitogens and antigens. We evaluated the ability of mAb to a framework determinant of HLA class I molecules to regulate T cell proliferation and interferon gamma (IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt;) production against leishmania, PPD, C. albicans and tetanus toxoid antigens in patients with tegumentary leishmaniasis and healthy subjects. The anti-major histocompatibility complex (MHC) mAb (W6/32) suppressed lymphocyte proliferation by 90% in cultures stimulated with &lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;CD3, but the suppression was variable in cultures stimulated with leishmania antigen. This suppression ranged from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA class I tegumentary leishmaniasis modulation of T cell response by HLA class I interferon-&lt; FONT FACE=Symbol&gt; G&lt; /FONT&gt; And HLA class I soluble antigen and HLA class I.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200010

Validation of the Beck Depression Inventory for a Portuguese-speaking Chinese community in Brazil
Provedor de dados: 56
Autores: Wang,Y.-P.; Andrade,L.H.; Gorenstein,C.
The objective of the present study was to investigate the psychometric properties and cross-cultural validity of the Beck Depression Inventory (BDI) among ethnic Chinese living in the city of São Paulo, Brazil. The study was conducted on 208 community individuals. Reliability and discriminant analysis were used to test the psychometric properties and validity of the BDI. Principal component analysis was performed to assess the BDI's factor structure for the total sample and by gender. The mean BDI score was lower (6.74, SD = 5.98) than observed in Western counterparts and showed no gender difference, good internal consistency (Cronbach's alpha 0.82), and high discrimination of depressive symptoms (75-100%). Factor analysis extracted two factors for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Depressive symptoms; Beck Depression Inventory; Psychometric properties; Validation of Depression Inventory; Portuguese-speaking Chinese; Factor analysis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300011

Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase
Provedor de dados: 56
Autores: Franco,C.R.C.; Rocha,H.A.O.; Trindade,E.S.; Santos,I.A.N.; Leite,E.L.; Veiga,S.S.; Nader,H.B.; Dietrich,C.P.
We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells. We used both wild-type (CHO-K1) cells and a mutant (CHO-745) which is deficient in the synthesis of proteoglycans due to lack of activity of xylosyl transferase. Using different amounts of wild-type and mutant cells, little adhesion was observed in the presence of laminin and type I collagen. However, when fibronectin or vitronectin was used as substrate, there was an enhancement in the adhesion of wild-type and mutant cells. Only CHO-K1 cells showed a time-dependent adhesion on type IV collagen. These results suggest that the two cell lines present different adhesive profiles. Several lines of experimental...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CHO cells; Extracellular matrix proteins; Proteoglycans; Glycosaminoglycans; Cell adhesion; Cell proliferation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800001

High specificity PCR screening for 22q11.2 microdeletion in three different ethnic groups
Provedor de dados: 56
Autores: Pereira,A.C.; Corrêa,R.F.R.; Mota,G.F.; Kim,C.A.; Mesquita,S.F.; Krieger,J.E.
Congenital heart defects are the most common of all human birth defects. Numerous studies have shown that a deletion within chromosome 22q11 is associated with DiGeorge syndrome and certain forms of sporadic congenital cardiovascular disease. We have determined the value of a PCR assay using markers D22S941, D22S944 and D22S264 designed for the screening of 22q11.2 deletion through consecutive homozygosity in an ethnically admixed urban population. The study population comprised 149 unrelated men and women from three different ethnic groups (white, mulatto and black). Test specificity for the overall population was estimated at 98.3%. We found no significant difference when comparing heterozygosity indices and ethnicity (P value = 0.43 (D22S944), 0.22...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DiGeorge syndrome; PCR screening; 22q11.2.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000012

Vitamin B1 and B6 in the malaria parasite: requisite or dispensable?
Provedor de dados: 56
Autores: Wrenger,C.; Knöckel,J.; Walter,R.D.; Müller,I.B.
Vitamins are essential compounds mainly involved in acting as enzyme co-factors or in response to oxidative stress. In the last two years it became apparent that apicomplexan parasites are able to generate B vitamers such as vitamin B1 and B6 de novo. The biosynthesis pathways responsible for vitamin generation are considered as drug targets, since both provide a high degree of selectivity due to their absence in the human host. This report updates the current knowledge about vitamin B1 and B6 biosynthesis in malaria and other apicomplexan parasites. Owing to the urgent need for novel antimalarials, the significance of the biosynthesis and salvage of these vitamins is critically discussed in terms of parasite survival and their exploitation for drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malaria; Apicomplexa; Pyridoxal phosphate synthase; Vitamin B6; Vitamin B1.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200001

A practical approach to assess leg muscle oxygenation during ramp-incremental cycle ergometry in heart failure
Provedor de dados: 56
Autores: Barroco,A.C.; Sperandio,P.A.; Reis,M.; Almeida,D.R.; Neder,J.A.
Heart failure is characterized by the inability of the cardiovascular system to maintain oxygen (O2) delivery (i.e., muscle blood flow in non-hypoxemic patients) to meet O2 demands. The resulting increase in fractional O2 extraction can be non-invasively tracked by deoxygenated hemoglobin concentration (deoxi-Hb) as measured by near-infrared spectroscopy (NIRS). We aimed to establish a simplified approach to extract deoxi-Hb-based indices of impaired muscle O2 delivery during rapidly-incrementing exercise in heart failure. We continuously probed the right vastus lateralis muscle with continuous-wave NIRS during a ramp-incremental cardiopulmonary exercise test in 10 patients (left ventricular ejection fraction &lt;35%) and 10 age-matched healthy males....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Exercise; Oxygen; Muscle; Near-infrared spectroscopy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200603

Outcomes and organ dysfunctions of critically ill patients with systemic lupus erythematosus and other systemic rheumatic diseases
Provedor de dados: 56
Autores: Ranzani,O.T.; Battaini,L.C.; Moraes,C.E.; Prada,L.F.L.; Pinaffi,J.V.; Giannini,F.P.; Shinjo,S.K.; Azevedo,L.C.P.; Park,M.
Our objective was to compare the pattern of organ dysfunctions and outcomes of critically ill patients with systemic lupus erythematosus (SLE) with patients with other systemic rheumatic diseases (SRD). We studied 116 critically ill SRD patients, 59 SLE and 57 other-SRD patients. The SLE group was younger and included more women. Respiratory failure (61%) and shock (39%) were the most common causes of ICU admission for other-SRD and SLE groups, respectively. ICU length-of-stay was similar for the two groups. The 60-day survival adjusted for the groups’ baseline imbalances was not different (P = 0.792). Total SOFA scores were equal for the two groups at admission and during ICU stay, although respiratory function was worse in the other-SRD group at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Critical illness; Rheumatic diseases; Systemic lupus erythematosus; Intensive care units; Multiple organ failure; Patient outcome assessment.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100016

Edaravone protects endotoxin-induced liver injury by inhibiting apoptosis and reducing proinflammatory cytokines
Provedor de dados: 56
Autores: Zong,L.; Yu,Q.H.; Du,Y.X.; Deng,X.M.
Studies have shown that edaravone may prevent liver injury. This study aimed to investigate the effects of edaravone on the liver injury induced by D-galactosamine (GalN) and lipopolysaccharide (LPS) in female BALB/c mice. Edaravone was injected into mice 30 min before and 4 h after GalN/LPS injection. The survival rate was determined within the first 24 h. Animals were killed 8 h after GalN/LPS injection, and liver injury was biochemically and histologically assessed. Hepatocyte apoptosis was measured by TUNEL staining; proinflammatory cytokines [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)] in the liver were assayed by ELISA; expression of caspase-8 and caspase-3 proteins was detected by Western blot assay; and caspase-3 activity was also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Edaravone; Apoptosis; Endotoxin; Liver injury; Inflammation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300231

Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism
Provedor de dados: 56
Autores: Hilsdorf,A.W.; Krieger,J.E.
Restriction fragment length polymorphism (RFLP) was used to examine the extent of mtDNA polymorphism among six strains of rats (Rattus norvegicus) - Wistar, Wistar Munich, Brown Norway, Wistar Kyoto, SHR and SHR-SP. A survey of 26 restriction enzymes has revealed a low level of genetic divergence among strains. The sites of cleavage by EcoRI, NcoI and XmnI were shown to be polymorphic. The use of these three enzymes allows the 6 strains to be classified into 4 haplotypes and identifies specific markers for each one. The percentage of sequence divergence among all pairs of haplotypes ranged from 0.035 to 0.33%, which is the result of a severe population constriction undergone by the strains. These haplotypes are easily demonstrable and therefore RFLP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Restriction fragment length polymorphism (RFLP); Rattus norvegicus; MtDNA; Laboratory rats; Genetic monitoring.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300003

Increased expression of ID2, PRELP and SMOC2 genes in patients with endometriosis
Provedor de dados: 56
Autores: Araujo,F.M.; Meola,J.; Rosa-e-Silva,J.C.; Paz,C.C.P.; Ferriani,R.A.; Nogueira,A.A.
Endometriosis is a benign, estrogen-dependent disease with symptoms such as pelvic pain and infertility, and it is characterized by the ectopic distribution of endometrial tissue. The expression of the ID2, PRELP and SMOC2 genes was compared between the endometrium of women without endometriosis in the proliferative phase of their menstrual cycle and the eutopic and ectopic endometrium of women with endometriosis in the proliferative phase. Paired tissue samples from 20 women were analyzed: 10 from endometrial and peritoneal endometriotic lesions and 10 from endometrial and ovarian endometriotic lesions. As controls, 16 endometrium samples were collected from women without endometriosis in the proliferative phase of menstrual cycle. Analysis was performed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; Endometriosis; PRELP; SMOC2; ID2; Real-time PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700605

Effects of L-arginine on the diaphragm muscle twitches elicited at different frequencies of nerve stimulation
Provedor de dados: 56
Autores: Queiroz,R.N.; Alves-Do-Prado,Wilson.
In rats, the nitric oxide (NO)-synthase pathway is present in skeletal muscle, vascular smooth muscle, and motor nerve terminals. Effects of NO were previously studied in rat neuromuscular preparations receiving low (0.2 Hz) or high (200 Hz) frequencies of stimulation. The latter frequency has always induced tetanic fade. However, in these previous studies we did not determine whether NO facilitates or impairs the neuromuscular transmission in preparations indirectly stimulated at frequencies which facilitate neuromuscular transmission. Thus, the present study was carried out to examine the effects of NO in rat neuromuscular preparations indirectly stimulated at 5 and 50 Hz. The amplitude of muscular contraction observed at the end (B) of a 10-s...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Skeletal muscle; Nitric oxide; L-arginine; Neuromuscular transmission; Tetanic fade; S-nitroso-N-acetylpenicillamine; Superoxide dismutase.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600020

Effects of vacuum sealing drainage on the treatment of cranial bone-exposed wounds in rabbits
Provedor de dados: 56
Autores: Chen,X.J.; Liu,S.; Gao,G.Z.; Yan,D.X.; Jiang,W.S.
This study was designed to assess the efficacy of vacuum sealing drainage (VSD) on skull exposure wounds in rabbits and to investigate the underlying mechanism of the process. Full-thickness excisional circular wounds 2×2 cm with or without periosteum involvement were created in 88 New Zealand white rabbits (mean body weight: 3.0±0.65 kg). Animals were randomly divided into 4 groups: periosteum-intact wounds treated with traditional dressing (p+control), periosteum-intact wounds treated with VSD (p+VSD), periosteum-lacking wounds treated with traditional dressing (p–control) and periosteum-lacking wounds treated with VSD (p–VSD). The wounds treated with traditional dressing were covered with Vaseline gauze, while VSD treatment was accompanied with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cranial bone-exposed wound; Vacuum sealing drainage; Periosteum; Wound healing; Efficacy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200602

Structure and function of the cystic fibrosis transmembrane conductance regulator
Provedor de dados: 56
Autores: Morales,M.M.; Capella,M.A.M.; Lopes,A.G.
Cystic fibrosis (CF) is a lethal autosomal recessive genetic disease caused by mutations in the CF transmembrane conductance regulator (CFTR). Mutations in the CFTR gene may result in a defective processing of its protein and alter the function and regulation of this channel. Mutations are associated with different symptoms, including pancreatic insufficiency, bile duct obstruction, infertility in males, high sweat Cl-, intestinal obstruction, nasal polyp formation, chronic sinusitis, mucus dehydration, and chronic Pseudomonas aeruginosa and Staphylococcus aureus lung infection, responsible for 90% of the mortality of CF patients. The gene responsible for the cellular defect in CF was cloned in 1989 and its protein product CFTR is activated by an increase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CFTR; Cystic fibrosis; Chloride channel; Function; Structure; Mutations.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800013

Magnesium chloride alone or in combination with diazepam fails to prevent hippocampal damage following transient forebrain ischemia
Provedor de dados: 56
Autores: Milani,H.; Lepri,E.R.; Giordani,F.; Favero-Filho,L.A.
In the central nervous system, magnesium ion (Mg2+) acts as an endogenous modulator of N-methyl-D-aspartate (NMDA)-coupled calcium channels, and may play a major role in the pathomechanisms of ischemic brain damage. In the present study, we investigated the effects of magnesium chloride (MgCl2, 2.5, 5.0 or 7.5 mmol/kg), either alone or in combination with diazepam (DZ), on ischemia-induced hippocampal cell death. Male Wistar rats (250-300 g) were subjected to transient forebrain ischemia for 15 min using the 4-vessel occlusion model. MgCl2 was applied systemically (sc) in single (1x, 2 h post-ischemia) or multiple doses (4x, 1, 2, 24 and 48 h post-ischemia). DZ was always given twice, at 1 and 2 h post-ischemia. Thus, ischemia-subjected rats were assigned...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebral ischemia; Hippocampal lesion; CA1 cell loss; Magnesium chloride; Diazepam; Neuroprotection.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000016

The use of reverse transcription-PCR for the diagnosis of X-linked chronic granulomatous disease
Provedor de dados: 56
Autores: Agudelo-Flórez,P.; López,J.A.; Redher,J.; Carneiro-Sampaio,M.M.S.; Costa-Carvalho,B.T.; Grumach,A.S.; Condino-Neto,A.
Chronic granulomatous disease (CGD) is an inherited disorder of the innate immune system characterized by a defective oxidative burst of phagocytes and subsequent impairment of their microbicidal activity. Mutations in one of the NADPH-oxidase components affect gene expression or function of this system, leading to the phenotype of CGD. Defects in gp91-phox lead to X-linked CGD, responsible for approximately 70% of CGD cases. Investigation of the highly heterogeneous genotype of CGD patients includes mutation analysis, Northern blot or Western blot assays according to the particular case. The aim of the present study was to use reverse transcription (RT)-PCR for the analysis of molecular defects responsible for X-linked CGD in eight Brazilian patients and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superoxide; Phagocytes; Primary immunodeficiency; Respiratory burst; Neutrophils; Human.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500001

Evidence for the presence of a kininogen-like species in a case of total deficiency of low and high molecular weight kininogens
Provedor de dados: 56
Autores: Veloso,D.
Low and high molecular weight kininogens (LK and HK), containing 409 and 626 amino acids with masses of ~65 and 120 kDa after glycosylation, respectively, are coded by a single gene mapped to the human chromosome 3 by alternative splicing of the transcribed mRNA. The NH2-termini Glu1-Thr383 region, identical in LK and HK, contains bradykinin (BK) moieties Arg363-Arg371. LK, HK and their kinin products Lys-BK and BK are involved in several biologic processes. They are evolutionarily conserved and only 7 patients, all apparently normal, have been reported to lack them. In one of these patients (Williams' trait), a codon mutation (Arg178 &lt;FONT FACE="Symbol"&gt;®&lt;/FONT&gt; stop) has been blamed for the absence of LK and HK. However, using Western blots...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kininogen deficiency; Kinins; Antibodies; Plasma; Kininogen-like species; Human and nonhuman primates.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700004

Cerebral vasomotor reactivity assessment using Transcranial Doppler and MRI with apnea test
Provedor de dados: 56
Autores: Herrera Campos,C.R.; Beltramini,G.C.; Avelar,W.M.; Lima,F.O.; Li,L.M.
Differently from previous studies that used Transcranial Doppler (TCD) and functional MRI (fMRI) for cerebral vasomotor reactivity (CVR) assessment in patients with carotid stenosis (CS), we assessed CVR using an identical stimulus, the Breath-Holding Test (BHT). We included 15 patients with CS and 7 age-matched controls to verify whether fMRI responded differently to BHT between groups and to calculate the agreement rate between tests. For TCD, impaired CVR was defined when the mean percentage increase on middle cerebral artery velocities was ≤31% on 3 consecutive 30-s apnea intercalated by 4-min normal breathing intervals. For fMRI, the percent variation on blood oxygen level-dependent (BOLD) signal intensity in the lentiform nucleus (LN) ipsilateral to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood oxygen level dependence; Transcranial Doppler; Carotid stenosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100704

Single-walled carbon nanotubes functionalized with sodium hyaluronate enhance bone mineralization
Provedor de dados: 56
Autores: Sá,M.A.; Ribeiro,H.J.; Valverde,T.M.; Sousa,B.R.; Martins-Júnior,P.A.; Mendes,R.M.; Ladeira,L.O.; Resende,R.R.; Kitten,G.T.; Ferreira,A.J.
The aim of this study was to evaluate the effects of sodium hyaluronate (HY), single-walled carbon nanotubes (SWCNTs) and HY-functionalized SWCNTs (HY-SWCNTs) on the behavior of primary osteoblasts, as well as to investigate the deposition of inorganic crystals on titanium surfaces coated with these biocomposites. Primary osteoblasts were obtained from the calvarial bones of male newborn Wistar rats (5 rats for each cell extraction). We assessed cell viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay and by double-staining with propidium iodide and Hoechst. We also assessed the formation of mineralized bone nodules by von Kossa staining, the mRNA expression of bone repair proteins, and the deposition of inorganic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoblast; Nanotechnology; Carbon nanotubes; Sodium hyaluronate; Tissue engineering; Osseointegration.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200602

Screening for carbohydrate-binding proteins in extracts of Uruguayan plants
Provedor de dados: 56
Autores: Plá,A.; Alonso,E.; Batista-Viera,F.; Franco Fraguas,L.
The presence of carbohydrate-binding proteins, namely lectins, ß-galactosidases and amylases, was determined in aqueous extracts of plants collected in Uruguay. Twenty-six extracts were prepared from 15 Uruguayan plants belonging to 12 Phanerogam families. Among them, 18 extracts caused hemagglutination (HAG) that was inhibited by mono- and disaccharides in 13 cases, indicating the presence of lectins. The other 8 extracts did not cause any HAG with the four systems used to detect HAG activity (rabbit and mouse red cells, trypsin-treated rabbit and mouse red cells). For the extracts prepared from Solanum commersonii, HAG activity and HAG inhibition were similar for those prepared from tubers, leaves and fruits, with the chitocompounds being responsible for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phanerogam families; Uruguayan plants; Asialofetuin-Sepharose; Carbohydrate-binding proteins; Lectins; SS-Galactosidases; Amylases.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700005

Temperature, air pollution, and mortality from myocardial infarction in São Paulo, Brazil
Provedor de dados: 56
Autores: Sharovsky,R.; César,L.A.M.; Ramires,J.A.F.
An increase in daily mortality from myocardial infarction has been observed in association with meteorological factors and air pollution in several cities in the world, mainly in the northern hemisphere. The objective of the present study was to analyze the independent effects of environmental variables on daily counts of death from myocardial infarction in a subtropical region in South America. We used the robust Poisson regression to investigate associations between weather (temperature, humidity and barometric pressure), air pollution (sulfur dioxide, carbon monoxide, and inhalable particulate), and the daily death counts attributed to myocardial infarction in the city of São Paulo in Brazil, where 12,007 fatal events were observed from 1996 to 1998....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiology; Myocardial infarction; Mortality; Temperature; Atmospheric pressure; Air pollution.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100009

Long-term efficacy of endovascular vs open surgical repair for complicated type-B aortic dissection: a single-center retrospective study and meta-analysis
Provedor de dados: 56
Autores: Zhu,Y.; Wang,B.; Meng,Q.; Liu,J.; Zhai,S.; He,J.
This study aimed to evaluate the long-term survival and risk factors of traditional open surgical repair (OSR) vs thoracic endovascular aneurysm repair (TEVAR) for complicated type-B aortic dissection (TBAD). A total of 118 inpatients (45 OSR vs 73 TEVAR) with TBAD were enrolled from January 2004 to January 2015. Kaplan-Meier curves and Cox proportional hazards analysis were performed to identify the long-term survival rate and independent predictors of survival, respectively. Meta-analysis was used to further explore the long-term efficacy of OSR and TEVAR in the eight included studies using Review Manager 5.2 software. An overall 10-year survival rate of 41.9% was found, and it was similar in the two groups (56.7% OSR vs 26.1% TEVAR; log-rank P=0.953)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aortic dissection; Open surgical repair; Type-B aortic dissection; Endovascular aortic repair.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600707

Association of adult weight gain and nonalcoholic fatty liver in a cross-sectional study in Wan Song Community, China
Provedor de dados: 56
Autores: Zhang,W.-J.; Chen,L.-L.; Zheng,J.; Lin,L.; Zhang,J.-Y.; Hu,X.
Our objective was to examine associations of adult weight gain and nonalcoholic fatty liver disease (NAFLD). Cross-sectional interview data from 844 residents in Wan Song Community from October 2009 to April 2010 were analyzed in multivariate logistic regression models to examine odds ratios (OR) and 95% confidence intervals (CI) between NAFLD and weight change from age 20. Questionnaires, physical examinations, laboratory examinations, and ultrasonographic examination of the liver were carried out. Maximum rate of weight gain, body mass index, waist circumference, waist-to-hip ratio, systolic blood pressure, diastolic blood pressure, fasting blood glucose, cholesterol, triglycerides, uric acid, and alanine transaminase were higher in the NAFLD group than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: China; Diabetes; Insulin resistance; Lipidosis; Nonalcoholic fatty liver disease; Obesity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200151

Endogenous angiotensin II modulates nNOS expression in renovascular hypertension
Provedor de dados: 56
Autores: Pereira,T.M.C.; Balarini,C.M.; Silva,I.V.; Cabral,A.M.; Vasquez,E.C.; Meyrelles,S.S.
Nitric oxide (NO) influences renal blood flow mainly as a result of neuronal nitric oxide synthase (nNOS). Nevertheless, it is unclear how nNOS expression is modulated by endogenous angiotensin II, an inhibitor of NO function. We tested the hypothesis that the angiotensin II AT1 receptor and oxidative stress mediated by NADPH oxidase contribute to the modulation of renal nNOS expression in two-kidney, one-clip (2K1C) hypertensive rats. Experiments were performed on male Wistar rats (150 to 170 g body weight) divided into 2K1C (N = 19) and sham-operated (N = 19) groups. nNOS expression in kidneys of 2K1C hypertensive rats (N = 9) was compared by Western blotting to that of 2K1C rats treated with low doses of the AT1 antagonist losartan (10 mg·kg-1·day-1; N...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Losartan; Tempol; Renovascular hypertension.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700014

The action of red wine and purple grape juice on vascular reactivity is independent of plasma lipids in hypercholesterolemic patients
Provedor de dados: 56
Autores: Coimbra,S.R.; Lage,S.H.; Brandizzi,L.; Yoshida,V.; da Luz,P.L.
Although red wine (RW) reduces cardiovascular risk, the mechanisms underlying the effect have not been identified. Correction of endothelial dysfunction by RW flavonoids could be one mechanism. We measured brachial artery reactivity by high-resolution ultrasonography, plasma lipids, glucose, adhesion molecules (ICAM-1 and VCAM), and platelet function in 16 hypercholesterolemic individuals (8 men and 8 women; mean age 51.6 ± 8.1 years) without other risk factors. Twenty-four normal subjects were used as controls for vascular reactivity. Subjects randomly received RW, 250 ml/day, or purple grape juice (GJ), 500 ml/day, for 14 days with an equal wash-out period. At baseline, all 16 subjects were hypercholesterolemic (mean LDL = 181.0 ± 28.7 mg/dl) but HDL,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Endothelial function; Flavonoids; Alcohol; Red wine and grape juice; Brachial artery dilation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900008

Computed tomography assessment of lung structure in patients undergoing cardiac surgery with cardiopulmonary bypass
Provedor de dados: 56
Autores: Rodrigues,R.R.; Sawada,A.Y.; Rouby,J.-J.; Fukuda,M.J.; Neves,F.H.; Carmona,M.J.; Pelosi,P.; Auler,J.O.; Malbouisson,L.M.S.
Hypoxemia is a frequent complication after coronary artery bypass graft (CABG) with cardiopulmonary bypass (CPB), usually attributed to atelectasis. Using computed tomography (CT), we investigated postoperative pulmonary alterations and their impact on blood oxygenation. Eighteen non-hypoxemic patients (15 men and 3 women) with normal cardiac function scheduled for CABG under CPB were studied. Hemodynamic measurements and blood samples were obtained before surgery, after intubation, after CPB, at admission to the intensive care unit, and 12, 24, and 48 h after surgery. Pre- and postoperative volumetric thoracic CT scans were acquired under apnea conditions after a spontaneous expiration. Data were analyzed by the paired Student t-test and one-way repeated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypoxemia; Computed tomography; Coronary artery bypass graft; Cardiopulmonary bypass.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600014

Alanyl-glutamine attenuates 5-fluorouracil-induced intestinal mucositis in apolipoprotein E-deficient mice
Provedor de dados: 56
Autores: Araújo,C.V.; Lazzarotto,C.R.; Aquino,C.C.; Figueiredo,I.L.; Costa,T.B.; de Oliveira Alves,L.A.; Ribeiro,R.A.; Bertolini,L.R.; Lima,A.A.M.; Brito,G.A.C.; Oriá,R.B.
Apolipoprotein E (APOE=gene, apoE=protein) is a known factor regulating the inflammatory response that may have regenerative effects during tissue recovery from injury. We investigated whether apoE deficiency reduces the healing effect of alanyl-glutamine (Ala-Gln) treatment, a recognized gut-trophic nutrient, during tissue recovery after 5-FU-induced intestinal mucositis. APOE-knockout (APOE-/-) and wild-type (APOE+/+) C57BL6J male and female mice (N=86) were given either Ala-Gln (100 mM) or phosphate buffered saline (PBS) by gavage 3 days before and 5 days after a 5-fluorouracil (5-FU) challenge (450 mg/kg, via intraperitoneal injection). Mouse body weight was monitored daily. The 5-FU cytotoxic effect was evaluated by leukometry. Intestinal villus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein E; 5-Fluorouracil; Alanyl-glutamine; Mucositis; Mice.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600493

Characterization of Plasmodium falciparum glutamate dehydrogenase-soluble antigen
Provedor de dados: 56
Autores: Rodríguez-Acosta,A.; Domínguez,N.G.; Aguilar,I.; Girón,M.E.
The major aim of this study was to characterize a soluble Plasmodium falciparum antigen from the plasma of malaria-infected humans and Plasmodium falciparum culture supernatants, using immunoabsorbent techniques and Western blotting. An Mr 60-kDa protein was isolated from the plasma of patients with Plasmodium falciparum malaria by affinity chromatography using rabbit anti-Proteus spp GDH(NADP+) serum as ligand. This protein, present in plasma of patients with acute Plasmodium falciparum infection, in Plasmodium falciparum culture supernatants, and in immune complexes, was tested with Plasmodium falciparum malaria hyperimmune serum from patients living in hyperendemic areas and rabbit anti-Proteus spp GDH(NADP+) serum prepared in the laboratory. In this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutamate-dehydrogenase; Plasmodium falciparum; Exoantigens; Soluble antigens; Malaria; Antigenic enzymes.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900008

An interleukin-33/ST2 signaling deficiency reduces overt pain-like behaviors in mice
Provedor de dados: 56
Autores: Magro,D.A.C.; Hohmann,M.S.N.; Mizokami,S.S.; Cunha,T.M.; Alves-Filho,J.C.; Casagrande,R.; Ferreira,S.H.; Liew,F.Y.; Cunha,F.Q.; Verri Jr,W.A.
Interleukin (IL)-33, the most recent member of the IL family of cytokines, signals through the ST2 receptor. IL-33/ST2 signaling mediates antigen challenge-induced mechanical hyperalgesia in the joints and cutaneous tissues of immunized mice. The present study asked whether IL-33/ST2 signaling is relevant to overt pain-like behaviors in mice. Acetic acid and phenyl-p-benzoquinone induced significant writhing responses in wild-type (WT) mice; this overt nociceptive behavior was reduced in ST2-deficient mice. In an antigen-challenge model, ST2-deficient immunized mice had reduced induced flinch and licking overt pain-like behaviors. In the formalin test, ST2-deficient mice also presented reduced flinch and licking responses, compared with WT mice. Naive WT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interleukin-33; ST2; Pain; Nociception; Inflammation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700601

Time sequence of changes in the responsiveness of glycogen breakdown to adrenergic agonists in perfused liver of rats with insulin-induced hypoglycemia
Provedor de dados: 56
Autores: Vardanega-Peicher,M.; Lopes,G.; Lima,F.B.; Curi,R.; Nakano,L.C.; Bazotte,R.B.
The time-course changes of the responsiveness of glycogen breakdown to a- and ß-adrenergic agonists during insulin-induced hypoglycemia (IIH) were investigated. Blood glucose levels were decreased prior to the alteration in the hepatic responsiveness to adrenergic agonists. The activation of hepatic glucose production and glycogenolysis by phenylephrine (2 µM) and isoproterenol (20 µM) was decreased in IIH. The changes in the responsiveness of glycogen catabolism were first observed for isoproterenol and later for phenylephrine. Hepatic ß-adrenergic receptors showed a higher degree of adrenergic desensitization than did a-receptors. Liver glycogen synthase activity, glycogen content and the catabolic effect of dibutyryl cyclic AMP (the ß-receptor second...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypoglycemia; Adrenergic responsiveness; Insulin-induced hypoglycemia; Hepatic glucose production; Glycogenolysis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700011

Mental rotation of anthropoid hands: a chronometric study
Provedor de dados: 56
Autores: Gawryszewski,L.G.; Silva-dos-Santos,C.F.; Santos-Silva,J.C.; Lameira,A.P.; Pereira Jr,A.
It has been shown that mental rotation of objects and human body parts is processed differently in the human brain. But what about body parts belonging to other primates? Does our brain process this information like any other object or does it instead maximize the structural similarities with our homologous body parts? We tried to answer this question by measuring the manual reaction time (MRT) of human participants discriminating the handedness of drawings representing the hands of four anthropoid primates (orangutan, chimpanzee, gorilla, and human). Twenty-four right-handed volunteers (13 males and 11 females) were instructed to judge the handedness of a hand drawing in palm view by pressing a left/right key. The orientation of hand drawings varied from...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Handedness recognition; Mental rotation; Manual reaction time; Motor imagery; Mirror neurons; Imitation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300013

The mutagenic and antimutagenic effects of the traditional phytoestrogen-rich herbs, Pueraria mirifica and Pueraria lobata
Provedor de dados: 56
Autores: Cherdshewasart,W.; Sutjit,W.; Pulcharoen,K.; Chulasiri,M.
Pueraria mirifica is a Thai phytoestrogen-rich herb traditionally used for the treatment of menopausal symptoms. Pueraria lobata is also a phytoestrogen-rich herb traditionally used in Japan, Korea and China for the treatment of hypertension and alcoholism. We evaluated the mutagenic and antimutagenic activity of the two plant extracts using the Ames test preincubation method plus or minus the rat liver mixture S9 for metabolic activation using Salmonella typhimurium strains TA98 and TA100 as indicator strains. The cytotoxicity of the two extracts to the two S. typhimurium indicators was evaluated before the mutagenic and antimutagenic tests. Both extracts at a final concentration of 2.5, 5, 10, or 20 mg/plate exhibited only mild cytotoxic effects. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phytoestrogen; Pueraria mirifica; Pueraria lobata; Mutagenic test; Antimutagenic test; Micronucleus test.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900008

Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein
Provedor de dados: 56
Autores: Passos,E.P.; Brugnara,L.; Facin,A.C.; Riffel,A.; Lima,G.R.; Freitas,V.; Brandelli,A.
The effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrosome reaction; Endometriosis; Infertility; Peritoneal fluid; Sperm.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100009

Circulating levels of adiponectin and extent of coronary artery disease in patients undergoing elective coronary angiography
Provedor de dados: 56
Autores: Souza,R.A.; Alves,C.M.R.; de Oliveira,C.S.V.; Reis,A.F.; Carvalho,A.C.
Adiponectin (APN), an adipose tissue-released adipokine with demonstrated anti-inflammatory and anti-atherogenic properties, is encoded by a gene whose polymorphisms are associated with presence of coronary artery disease (CAD). Serum APN levels are inversely related with presence and complexity of CAD. Within this context, we sought to compare levels of total APN and its high molecular weight form (HMW APN) according to clinical presentation and extent of CAD in patients undergoing elective cardiac catheterization. From March 2008 to June 2010, clinical data and blood samples for APN and HMW APN measurements were collected from 415 subjects undergoing cardiac catheterization at two tertiary centers. CAD extent was estimated by the number of coronary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipose tissue; Adipokines; Adiponectin; Coronary artery disease; Atherosclerosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200604

The importance of the Thr17 residue of phospholamban as a phosphorylation site under physiological and pathological conditions
Provedor de dados: 56
Autores: Mattiazzi,A.; Mundiña-Weilenmann,C.; Vittone,L.; Said,M.; Kranias,E.G.
The sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA2a) is under the control of an SR protein named phospholamban (PLN). Dephosphorylated PLN inhibits SERCA2a, whereas phosphorylation of PLN at either the Ser16 site by PKA or the Thr17 site by CaMKII reverses this inhibition, thus increasing SERCA2a activity and the rate of Ca2+ uptake by the SR. This leads to an increase in the velocity of relaxation, SR Ca2+ load and myocardial contractility. In the intact heart, ß-adrenoceptor stimulation results in phosphorylation of PLN at both Ser16 and Thr17 residues. Phosphorylation of the Thr17 residue requires both stimulation of the CaMKII signaling pathways and inhibition of PP1, the major phosphatase that dephosphorylates PLN. These two prerequisites appear to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phospholamban; Thr17 site phosphorylation; SS-adrenergic stimulation; Acidosis; Ischemia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500001

The efficacy of moxifloxacin-based triple therapy in treatment of Helicobacter pylori infection: a systematic review and meta-analysis of randomized clinical trials
Provedor de dados: 56
Autores: Zhang,G.; Zou,J.; Liu,F.; Bao,Z.; Dong,F.; Huang,Y.; Yin,S.
Recent evidence shows that moxifloxacin could exert an antimicrobial effect against Helicobacter pylori in both in vitroand in vivo models. To systematically evaluate whether moxifloxacin-containing triple therapy could improve eradication rates and reduce side effects in first-line or second-line anti-H. pyloritreatment, eligible articles were identified by searches of electronic databases. We included all randomized trials comparing moxifloxacin-based triple therapy with standard triple or quadruple therapy during H. pylori eradication treatment. Statistical analysis was performed with Review Manager 5.0.10. Subanalysis/sensitivity analysis was also performed. We identified seven randomized trials (n=1263). Pooled H. pylori eradication rates were 79.03%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Moxifloxacin; Helicobacter pylori; Eradication rate; Side effects; Meta-analysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700607

DNA methylation regulates expression of VEGF-C, and S-adenosylmethionine is effective for VEGF-C methylation and for inhibiting cancer growth
Provedor de dados: 56
Autores: Da,M.X.; Zhang,Y.B.; Yao,J.B.; Duan,Y.X.
DNA hypomethylation may activate oncogene transcription, thus promoting carcinogenesis and tumor development. S-adenosylmethionine (SAM) is a methyl donor in numerous methylation reactions and acts as an inhibitor of intracellular demethylase activity, which results in hypermethylation of DNA. The main objectives of this study were to determine whether DNA hypomethylation correlated with vascular endothelial growth factor-C (VEGF-C) expression, and the effect of SAM on VEGF-C methylation and gastric cancer growth inhibition. VEGF-C expression was assayed by Western blotting and RT-qPCR in gastric cancer cells, and by immunohistochemistry in tumor xenografts. VEGF-C methylation was assayed by bisulfite DNA sequencing. The effect of SAM on cell apoptosis was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: VEGF-C; Methylation; S-adenosylmethionine; Gastric cancer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201021

Time course of training-induced microcirculatory changes and of vegf expression in skeletal muscles of spontaneously hypertensive female rats
Provedor de dados: 56
Autores: Amaral,S.L.; Sanchez,L.S.; Chang,A.J.B.A.; Rossoni,L.V.; Michelini,L.C.
Exercise-induced vessel changes modulate arterial pressure (AP) in male spontaneously hypertensive rats (SHR). Vascular endothelial growth factor (VEGF) is important for angiogenesis of skeletal muscle. The present study evaluated the time course of VEGF and angiogenesis after short- and long-term exercise training of female SHR and Wistar Kyoto (WKY) rats, 8-9 weeks (200-250 g). Rats were allocated to daily training or remained sedentary for 3 days (N = 23) or 13 weeks (N = 23). After training, the carotid artery was catheterized for AP measurements. Locomotor (tibialis anterior and gracilis) and non-locomotor skeletal muscles (temporalis) were harvested and prepared for histologic and protein expression analyses. Training increased treadmill performance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High blood pressure; Exercise training; Capillary density; VEGF expression; Skeletal muscle angiogenesis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500012

CD47 expression is decreased in hematopoietic progenitor cells in patients with myelofibrosis
Provedor de dados: 56
Autores: Nonino,A.; Nascimento,J.M.; Mascarenhas,C.C.; Mazzeu,J.F.; Pereira,R.W.; Jacomo,R.H.
Myelofibrosis (MF) is characterized by increased circulating hematopoietic progenitor cells (HPCs), abnormal cytokine levels, and the survival advantage of neoplastic progenitors over their normal counterparts, which leads to progressive disappearance of polyclonal hematopoiesis. CD47 is a surface glycoprotein with many functions, such as acting as a phagocytosis inhibitor of the expressing cell, that is increased in normal hematopoietic stem and progenitor cells mobilized into the blood and several human cancer-initiating cells, such as in acute myeloid leukemia. We compared CD47 expression in hematopoietic stem and progenitor cells of patients with MF and controls and found it to be decreased in progenitors of MF. Exposure of control HPCs to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myeloproliferative disorders; Primary myelofibrosis; Hematopoietic stem cells; Neoplastic stem cells; Antigens; CD47.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100610

Systemic and regional hemodynamic effects of enalaprilat infusion in experimental normotensive sepsis
Provedor de dados: 56
Autores: Rahal,L.; Garrido,A.G.; Cruz Jr.,R.J.; Rocha e Silva,M.; Poli-de-Figueiredo,L.F.
Angiotensin-converting enzyme inhibitors have been shown to improve splanchnic perfusion in distinct shock states. We hypothesized that enalaprilat potentiates the benefits of early fluid resuscitation in severe experimental sepsis, particularly in the splanchnic region. Anesthetized and mechanically ventilated mongrel dogs received an intravenous infusion of live Escherichia coli over a period of 30 min. Thereafter, two interventions were performed: fluid infusion (normal saline, 32 mL/kg over 30 min) and enalaprilat infusion (0.02 mg kg-1 min-1 for 60 min) in randomized groups. The following groups were studied: controls (fluid infusion, N = 4), E1 (enalaprilat infusion followed by fluid infusion, N = 5) and E2 (fluid infusion followed by enalaprilat...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac output; Escherichia coli; Enalaprilat; Portal vein blood flow; Septic shock.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900008

Developmental and behavioral effects of postnatal amitraz exposure in rats
Provedor de dados: 56
Autores: Palermo-Neto,J.; Sakaté,M.; Flório,J.C.
The effects of postnatal amitraz exposure on physical and behavioral parameters were studied in Wistar rats, whose lactating dams received the pesticide (10 mg/kg) orally on days 1, 4, 7, 10, 13, 16 and 19 of lactation; control dams received distilled water (1 ml/kg) on the same days. A total of 18 different litters (9 of them control and 9 experimental) born after a 21-day gestation were used. The results showed that the median effective time (ET50) for fur development, eye opening, testis descent and onset of the startle response were increased in rats postnatally exposed to amitraz (2.7, 15.1, 21.6 and 15.3 days, respectively) compared to those of the control pups (1.8, 14.0, 19.9 and 12.9 days, respectively). The ages of incisor eruption, total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pesticide neurotoxicology; Amitraz; Lactation; Behavioral teratology.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800013

Hemochromatosis (HFE) gene mutations in Brazilian chronic hemodialysis patients
Provedor de dados: 56
Autores: Perícole,F.V.; Alves,M.A.V.R.; Saad,S.T.O.; Costa,F.F.
Patients with chronic renal insufficiency (CRI) have reduced hemoglobin levels, mostly as a result of decreased kidney production of erythropoietin, but the relation between renal insufficiency and the magnitude of hemoglobin reduction has not been well defined. Hereditary hemochromatosis is an inherited disorder of iron metabolism. The importance of the association of hemochromatosis with treatment for anemia among patients with CRI has not been well described. We analyzed the frequency of the C282Y and H63D mutations in the HFE gene in 201 Brazilian individuals with CRI undergoing hemodialysis. The analysis of the effects of HFE mutations on iron metabolism and anemia with biochemical parameters was possible in 118 patients of this study (hemoglobin,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HFE; Chronic renal insufficiency; Anemia; Iron metabolism.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900005

Effects of losartan combined with exercise training in spontaneously hypertensive rats
Provedor de dados: 56
Autores: Azevedo,L.F.; Brum,P.C.; Mattos,K.C.; Junqueira,C.M.; Rondon,M.U.P.B.; Barretto,A.C.P.; Negrao,C.E.
We investigate whether combined treatment with losartan, an angiotensin II receptor blocker, and exercise training (ET) in spontaneously hypertensive rats (SHR) would have an additive effect in reducing hypertension and improving baroreflex sensitivity when compared with losartan alone. Male SHR (8 weeks old) were assigned to 3 groups: sedentary placebo (SP, N = 16), sedentary under losartan treatment (SL, N = 11; 10 mg kg-1 day-1, by gavage), and ET under losartan treatment (TL, N = 10). ET was performed on a treadmill 5 days/week for 60 min at 50% of peak VO2, for 18 weeks. Blood pressure (BP) was measured with a catheter inserted into the carotid artery, and cardiac output with a microprobe placed around the ascending aorta. The baroreflex control of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spontaneously hypertensive rats; Exercise training; Losartan; Baroreflex sensitivity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100018

TNF-α inhibits SCF, ghrelin, and substance P expressions through the NF-κB pathway activation in interstitial cells of Cajal
Provedor de dados: 56
Autores: Ren,Keyu; Yong,Chunming; Yuan,Hao; Cao,Bin; Zhao,Kun; Wang,Jin.
Ulcerative colitis is a chronic inflammatory disease of the colon where intestinal motility is disturbed. Interstitial cells of Cajal (ICC) are required to maintain normal intestinal motility. In the present study, we assessed the effect of tumor necrosis factor-alpha (TNF-α) on viability and apoptosis of ICC, as well as on the expression of stem cell factor (SCF), ghrelin, and substance P. ICC were derived from the small intestines of Swiss albino mice. Cell viability and apoptosis were measured using CCK-8 assay and flow cytometry, respectively. ELISA was used to measure the concentrations of IL-1β, IL-6, ghrelin, substance P, and endothelin-1. Quantitative RT-PCR was used to measure the expression of SCF. Western blotting was used to measure the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TNF-α; SCF; Ghrelin; Substance P; NF-κB; Interstitial cells of Cajal.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600610

Immune cells and oxidative stress in the endotoxin tolerance mouse model
Provedor de dados: 56
Autores: Melo,E.S.; Barbeiro,H.V.; Ariga,S.; Goloubkova,T.; Curi,R.; Velasco,I.T.; Vasconcelos,D.; Soriano,F.G.
Sepsis is a systemic inflammatory response that can lead to tissue damage and death. In order to increase our understanding of sepsis, experimental models are needed that produce relevant immune and inflammatory responses during a septic event. We describe a lipopolysaccharide tolerance mouse model to characterize the cellular and molecular alterations of immune cells during sepsis. The model presents a typical lipopolysaccharide tolerance pattern in which tolerance is related to decreased production and secretion of cytokines after a subsequent exposure to a lethal dose of lipopolysaccharide. The initial lipopolysaccharide exposure also altered the expression patterns of cytokines and was followed by an 8- and a 1.5-fold increase in the T helper 1 and 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sepsis; Cytokines; Immune tolerance; Lipopolysaccharide; Murine sepsis model; T helper cells.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100009

Cl- and regulation of pH by MDCK-C11 cells
Provedor de dados: 56
Autores: Tararthuch,A.L.; Fernandez,R.; Malnic,G.
The interaction between H+ extrusion via H+-ATPase and Cl- conductance was studied in the C11 clone of MDCK cells, akin to the intercalated cells of the collecting duct. Cell pH (pHi) was measured by fluorescence microscopy using the fluorescein-derived probe BCECF-AM. Control recovery rate measured after a 20 mM NH4Cl acid pulse was 0.136 ± 0.008 pH units/min (dpHi/dt) in Na+ Ringer and 0.032 ± 0.003 in the absence of Na+ (0 Na+). With 0 Na+ plus the Cl- channel inhibitor NPPB (10 µM), recovery was reduced to 0.014 ± 0.001 dpHi/dt. 8-Br-cAMP, known to activate CFTR Cl- channels, increased dpHi/dt in 0 Na+ to 0.061 ± 0.009 and also in the presence of 46 nM concanamycin and 50 µM Schering 28080. Since it is thought that the Cl- dependence of H+-ATPase might...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell pH; CAMP; ATP; H+-ATPase; Valinomycin; Cl-.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500012

Sec61alpha synthesis is enhanced during translocation of nascent chains of collagen type IV in F9 teratocarcinoma cells after retinoic acid treatment
Provedor de dados: 56
Autores: Ferreira,L.R.; Velano,C.E.E.; Braga,E.C.; Paula,C.C.; Martéli Junior,H.; Sauk,J.J.
Nascent procollagen peptides and other secretory proteins are transported across the endoplasmic reticulum (ER) membrane through a protein-conducting channel called translocon. Sec61alpha, a multispanning membrane translocon protein, has been implicated as being essential for translocation of polypeptide chains into the cisterns of the ER. Sec61alpha forms a protein complex with collagen and Hsp47, an ER-resident heat shock protein that binds specifically to collagen. However, it is not known whether Sec61alpha is ubiquitously produced in collagen-producing F9 teratocarcinoma cells or under heat shock treatment. Furthermore, the production and utilization of Sec61alpha may depend on the stage of cell differentiation. Cultured F9 teratocarcinoma cells are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sec61alpha; Collagen type IV; Retinoic acid; F9 cells; Differentiation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100005

Effects of pregnancy and protein-energy malnutrition on monooxygenase O-dealkylation activity in rat liver microsomes
Provedor de dados: 56
Autores: Kuriyama,S.N.; De-Oliveira,A.C.A.X.; Fidalgo-Neto,A.A.; Paumgartten,F.J.R.
Xenobiotic metabolism is influenced by a variety of physiological and environmental factors including pregnancy and nutritional status of the individual. Pregnancy has generally been reported to cause a depression of hepatic monooxygenase activities. Low-protein diets and protein-energy malnutrition have also been associated with a reduced activity of monooxygenases in nonpregnant animals. We investigated the combined effects of pregnancy and protein-energy malnutrition on liver monooxygenase O-dealkylation activity. On pregnancy day 0 rats were assigned at random to a group fed ad libitum (well-nourished, WN) or to a malnourished group (MN) which received half of the WN food intake (12 g/day). WN and MN rats were killed on days 0 (nonpregnant), 11 or 20...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytochrome P450; Monooxygenases; Xenobiotic metabolism; Undernutrition; Gestation; Biotransformation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100014

Effect of an aqueous extract of Scoparia dulcis on blood glucose, plasma insulin and some polyol pathway enzymes in experimental rat diabetes
Provedor de dados: 56
Autores: Latha,M.; Pari,L.
The effects of an aqueous extract of the plant Scoparia dulcis (200 mg/kg) on the polyol pathway and lipid peroxidation were examined in the liver of streptozotocin adult diabetic male albino Wistar rats. The diabetic control rats (N = 6) presented a significant increase in blood glucose, sorbitol dehydrogenase, glycosylated hemoglobin and lipid peroxidation markers such as thiobarbituric acid reactive substances (TBARS) and hydroperoxides, and a significant decrease in plasma insulin and antioxidant enzymes such as glutathione peroxidase (GPx), glutathione-S-transferase (GST) and reduced glutathione (GSH) compared to normal rats (N = 6). Scoparia dulcis plant extract (SPEt, 200 mg kg-1 day-1) and glibenclamide (600 µg kg-1 day-1), a reference drug, were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione peroxidase; Glutathione-S-transferase; Insulin; Lipid peroxidation; Scoparia dulcis; Sorbitol dehydrogenase; Diabetes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400015

Demographic and metabolic characteristics of individuals with progressive glucose tolerance
Provedor de dados: 56
Autores: Mendes,A.L.; Santos,M.L.; Padovani,C.R.; Pimenta,W.P.
We evaluated changes in glucose tolerance of 17 progressors and 62 non-progressors for 9 years to improve our understanding of the pathogenesis of type 2 diabetes mellitus. Changes in anthropometric measurements and responses to an oral glucose tolerance test (OGTT) were analyzed. We identified 14 pairs of individuals, one from each group, who were initially normal glucose tolerant and were matched for gender, age, weight, and girth. We compared initial plasma glucose and insulin curves (from OGTT), insulin secretion (first and second phases) and insulin sensitivity indices (from hyperglycemic clamp assay) for both groups. In the normal glucose tolerant phase, progressors presented: 1) a higher OGTT blood glucose response with hyperglycemia in the second...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Impaired glucose tolerance; Impaired insulin secretion; Normal glucose tolerance; Hyperglycemic clamp; Oral glucose tolerance test.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300009

Evaluation of two experimental models of hepatic encephalopathy in rats
Provedor de dados: 56
Autores: García-Moreno,L.M.; Conejo,N.M.; González-Pardo,H.; Aller,M.A.; Nava,M.P.; Arias,J.; Arias,J.L.
The serious neuropsychological repercussions of hepatic encephalopathy have led to the creation of several experimental models in order to better understand the pathogenesis of the disease. In the present investigation, two possible causes of hepatic encephalopathy, cholestasis and portal hypertension, were chosen to study the behavioral impairments caused by the disease using an object recognition task. This working memory test is based on a paradigm of spontaneous delayed non-matching to sample and was performed 60 days after surgery. Male Wistar rats (225-250 g) were divided into three groups: two experimental groups, microsurgical cholestasis (N = 20) and extrahepatic portal hypertension (N = 20), and a control group (N = 20). A mild alteration of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholestasis; Portal hypertension; Encephalopathy; Behavior; Recognition memory test.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100019

[RETRACTED ARTICLE] Comparison of Arndt-endobronchial blocker plus laryngeal mask airway with left-sided double-lumen endobronchial tube in one-lung ventilation in thoracic surgery in the morbidly obese
Provedor de dados: 56
Autores: Zhang,Z.J.; Zheng,M.L.; Nie,Y.; Niu,Z.Q.
This study aimed to evaluate the feasibility and performance of Arndt-endobronchial blocker (Arndt) combined with laryngeal mask airway (LMA) compared with left-sided double-lumen endobronchial tube (L-DLT) in morbidly obese patients in one-lung ventilation (OLV). In a prospective, randomized double-blind controlled clinical trial, 80 morbidly obese patients (ASA I-III, aged 20–70) undergoing general anesthesia for elective thoracic surgeries were randomly allocated into groups Arndt (n=40) and L-DLT (n=40). In group Arndt, a LMA™ Proseal was placed followed by an Arndt-endobronchial blocker. In group L-DLT, patients were intubated with a left-sided double-lumen endotracheal tube. Primary endpoints were the airway establishment, ease of insertion,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laryngeal mask airway; Arndt-endobronchial blocker; L-DLT; One-lung ventilation; Thoracic surgery; Morbidly obese.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200617

Stability of relative oxygen pulse curve during repeated maximal cardiopulmonary testing in professional soccer players
Provedor de dados: 56
Autores: Perim,R.R.; Signorelli,G.R.; Araújo,C.G.S.
During cardiopulmonary exercise testing (CPET), stroke volume can be indirectly assessed by O2 pulse profile. However, for a valid interpretation, the stability of this variable over time should be known. The objective was to analyze the stability of the O2 pulse curve relative to body mass in elite athletes. VO2, heart rate (HR), and relative O2 pulse were compared at every 10% of the running time in two maximal CPETs, from 2005 to 2010, of 49 soccer players. Maximal values of VO2 (63.4 ± 0.9 vs 63.5 ± 0.9 mL O2•kg-1•min-1), HR (190 ± 1 vs188 ± 1 bpm) and relative O2 pulse (32.9 ± 0.6 vs 32.6 ± 0.6 mL O2•beat-1•kg-1) were similar for the two CPETs (P &amp;gt; 0.05), while the final treadmill velocity increased from 18.5 ± 0.9 to 18.9 ± 1.0 km/h (P &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise testing; Sport cardiology; Maximal oxygen uptake; Stroke volume; Ramp protocol; Heart rate.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700014

A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics
Provedor de dados: 56
Autores: Lavich,T.R.; Cordeiro,R.S.B.; Silva,P.M.R.; Martins,M.A.
It is widely accepted that the classical constant-temperature hot-plate test is insensitive to cyclooxygenase inhibitors. In the current study, we developed a variant of the hot-plate test procedure (modified hot-plate (MHP) test) to measure inflammatory nociception in freely moving rats and mice. Following left and right hind paw stimulation with a phlogogen and vehicle, respectively, the animals were placed individually on a hot-plate surface at 51ºC and the withdrawal latency for each paw was determined simultaneously in measurements performed at 15, 60, 180, and 360 min post-challenge. Plantar stimulation of rats (250 and 500 µg/paw) and mice (125-500 µg/paw) with carrageenan led to a rapid hyperalgesic response of the ipsilateral paw that reached a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperalgesia; Hargreaves' test; Modified hot-plate test; Carrageenan; Prostaglandin E2; Indomethacin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300016

Baroreflex control of sympathetic activity in experimental hypertension
Provedor de dados: 56
Autores: Irigoyen,M.C.C.; Krieger,E.M.
The arterial baroreceptor reflex system is one of the most powerful and rapidly acting mechanisms for controlling arterial pressure. The purpose of the present review is to discuss data relating sympathetic activity to the baroreflex control of arterial pressure in two different experimental models: neurogenic hypertension by sinoaortic denervation (SAD) and high-renin hypertension by total aortic ligation between the renal arteries in the rat. SAD depresses baroreflex regulation of renal sympathetic activity in both the acute and chronic phases. However, increased sympathetic activity (100%) was found only in the acute phase of sinoaortic denervation. In the chronic phase of SAD average discharge normalized but the pattern of discharges was different from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baroreflex; Sympathetic activity; Sinoaortic denervation; High-renin hypertension.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900015

Synergistic antitumor effect of TRAIL and adriamycin on the human breast cancer cell line MCF-7
Provedor de dados: 56
Autores: Cui,D.D.; Huang,Y.; Mao,S.H.; Chen,S.C.; Qiu,M.; Ji,L.L.; Yi,C.
The aim of the present study was to determine the effect of the combination of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and adriamycin (ADM) on the human breast cancer cell line MCF-7 and to identify potential mechanisms of apoptosis. Cell viability was analyzed by the MTT assay and the synergistic effect was assessed by the Webb coefficient. Apoptosis was quantified using the annexin V-FITC and propidium iodide staining flow cytometry. The mRNA expression of TRAIL receptors was measured by RT-PCR. Changes in the quantities of Bax and caspase-9 proteins were determined by Western blot. MCF-7 cells were relatively resistant to TRAIL (IC50 &gt;10 µg/mL), while MCF-7 cells were sensitive to ADM (IC50 &lt;10 µg/mL). A subtoxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TRAIL; TRAIL receptors; Breast cancer; Adriamycin; Apoptosis; Synergism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900013

Up-regulated expression of CD147 gene in malignant bone tumor and the possible induction mechanism during osteoclast formation
Provedor de dados: 56
Autores: Qiao,Suchi; Liu,Chang; Xu,Weijie; AZhaTi,WuBuLi; Li,Cheng; Wang,Zhiwei.
It is increasingly evident that the microenvironment of bone can influence cancer phenotype in many ways that favor growth in bone. CD147, a transmembrane protein of the immunoglobulin (Ig) superfamily, was identified independently in different species and has many designations across different species. However, expression levels of CD147 mRNA in bone cancer have not been described. In this study, we have used real-time fluorescence quantification (RT-PCR) to demonstrate CD147 expression in malignant bone cancer and benign bone tumor tissues. The results suggested that the expression of CD147 gene was significantly up-regulated in malignant bone cancer. Moreover, we found that over-expressed RANKL progressively enhanced osteoclast formation up to 48 h,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone cancer; RT-PCR; Western blot; RANKL; Human.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900608

Metronomic chemotherapy in the neoadjuvant setting: results of two parallel feasibility trials (TraQme and TAME) in patients with HER2+ and HER2− locally advanced breast cancer
Provedor de dados: 56
Autores: Petry,V.; Gagliato,D.M.; Leal,A.I.C.; Arai,R.J.; Longo,E.; Andrade,F.; Ricci,M.D.; Piato,J.R.; Barroso-Sousa,R.; Hoff,P.M.; Mano,M.S.
Neoadjuvant chemotherapy has practical and theoretical advantages over adjuvant chemotherapy strategy in breast cancer (BC) management. Moreover, metronomic delivery has a more favorable toxicity profile. The present study examined the feasibility of neoadjuvant metronomic chemotherapy in two cohorts [HER2+ (TraQme) and HER2− (TAME)] of locally advanced BC. Twenty patients were prospectively enrolled (TraQme, n=9; TAME, n=11). Both cohorts received weekly paclitaxel at 100 mg/m2 during 8 weeks followed by weekly doxorubicin at 24 mg/m2 for 9 weeks in combination with oral cyclophosphamide at 100 mg/day (fixed dose). The HER2+ cohort received weekly trastuzumab. The study was interrupted because of safety issues. Thirty-six percent of patients in the TAME...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Metronomic chemotherapy; HER2+.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500479

Autonomic control of cardiorespiratory interactions in fish, amphibians and reptiles
Provedor de dados: 56
Autores: Taylor,E.W.; Leite,C.A.C.; Skovgaard,N.
Control of the heart rate and cardiorespiratory interactions (CRI) is predominantly parasympathetic in all jawed vertebrates, with the sympathetic nervous system having some influence in tetrapods. Respiratory sinus arrhythmia (RSA) has been described as a solely mammalian phenomenon but respiration-related beat-to-beat control of the heart has been described in fish and reptiles. Though they are both important, the relative roles of feed-forward central control and peripheral reflexes in generating CRI vary between groups of fishes and probably between other vertebrates. CRI may relate to two locations for the vagal preganglionic neurons (VPN) and in particular cardiac VPN in the brainstem. This has been described in representatives from all vertebrate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiorespiratory interactions; Autonomic nervous system; Cardiac vagal preganglionic neurons; Central interactions; Peripheral reflex control; Non-adrenergic-non-cholinergic (NANC) control of heart rate.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700001

New paradigms and ethics
Provedor de dados: 56
Autores: Castro,L.A.B. de.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500001

Control of respiration in fish, amphibians and reptiles
Provedor de dados: 56
Autores: Taylor,E.W.; Leite,C.A.C.; McKenzie,D.J.; Wang,T.
Fish and amphibians utilise a suction/force pump to ventilate gills or lungs, with the respiratory muscles innervated by cranial nerves, while reptiles have a thoracic, aspiratory pump innervated by spinal nerves. However, fish can recruit a hypobranchial pump for active jaw occlusion during hypoxia, using feeding muscles innervated by anterior spinal nerves. This same pump is used to ventilate the air-breathing organ in air-breathing fishes. Some reptiles retain a buccal force pump for use during hypoxia or exercise. All vertebrates have respiratory rhythm generators (RRG) located in the brainstem. In cyclostomes and possibly jawed fishes, this may comprise elements of the trigeminal nucleus, though in the latter group RRG neurons have been located in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vertebrates; Control of respiration; Respiratory rhythm generation; Water and air breathing; Chemoreceptors; Mechanoreceptors.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500001

Behavioral changes induced by cocaine in mice are modified by a hyperlipidic diet or recombinant leptin
Provedor de dados: 56
Autores: Erhardt,E.; Zibetti,L.C.E.; Godinho,J.M.; Bacchieri,B.; Barros,H.M.T.
The objective of the present study was to determine if the acute behavioral effects of cocaine acutely administered intraperitoneally (ip) at doses of 5, 10 and 20 mg/kg on white male CF1 mice, 90 days of age, would be influenced by leptin acutely administered ip (at doses of 5, 10 and 20 µg/kg) or by endogenous leptin production enhanced by a high-fat diet. The acute behavioral effects of cocaine were evaluated in open-field, elevated plus-maze and forced swimming tests. Results were compared between a group of 80 mice consuming a balanced diet and a high-fat diet, and a group of 80 mice fed a commercially available rodent chow formula (Ralston Purina) but receiving recombinant leptin (rLeptin) or saline ip. Both the high-fat-fed and rLeptin-treated mice...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mice; Cocaine; Leptin; Hyperlipidic diet; Interaction.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200014

Carbon monoxide: from toxin to endogenous modulator of cardiovascular functions
Provedor de dados: 56
Autores: Johnson,R.A.; Kozma,F.; Colombari,E.
Carbon monoxide (CO) is a pollutant commonly recognized for its toxicological attributes, including CNS and cardiovascular effects. But CO is also formed endogenously in mammalian tissues. Endogenously formed CO normally arises from heme degradation in a reaction catalyzed by heme oxygenase. While inhibitors of endogenous CO production can raise arterial pressure, heme loading can enhance CO production and lead to vasodepression. Both central and peripheral tissues possess heme oxygenases and generate CO from heme, but the inability of heme substrate to cross the blood brain barrier suggests the CNS heme-heme oxygenase-CO system may be independent of the periphery. In the CNS, CO apparently acts in the nucleus tractus solitarii (NTS) promoting changes in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbon monoxide; Heme; Heme oxygenase; Blood pressure; Central nervous system; Vascular tone.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100001

Cardiovascular effects of the intracerebroventricular injection of adrenomedullin: roles of the peripheral vasopressin and central cholinergic systems
Provedor de dados: 56
Autores: Cam-Etoz,B.; Isbil-Buyukcoskun,N.; Ozluk,K.
Our objective was to investigate in conscious Sprague-Dawley (6-8 weeks, 250-300 g) female rats (N = 7 in each group) the effects of intracerebroventricularly (icv) injected adrenomedullin (ADM) on blood pressure and heart rate (HR), and to determine if ADM and calcitonin gene-related peptide (CGRP) receptors, peripheral V1 receptors or the central cholinergic system play roles in these cardiovascular effects. Blood pressure and HR were observed before and for 30 min following drug injections. The following results were obtained: 1) icv ADM (750 ng/10 µL) caused an increase in both blood pressure and HR (DMAP = 11.8 ± 2.3 mmHg and ΔHR = 39.7 ± 4.8 bpm). 2) Pretreatment with a CGRP receptor antagonist (CGRP8-37) and ADM receptor antagonist (ADM22-52)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenomedullin; Blood pressure; Vasopressin; Atropine; Mecamylamine.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300011

Sleep disorder or simple sleep ontogeny? Tendency for morningness is associated with worse sleep quality in the elderly
Provedor de dados: 56
Autores: Barbosa,A.A.; Miguel,M.A.L.; Tufik,S.; Sabino,F.C.; Cendoroglo,M.S.; Pedrazzoli,M.
The objective of this study was to evaluate the alterations in sleep and circadian parameters during the aging process. The study sample comprises volunteers older than 18 up to 90 years of age that answered the Pittsburgh Sleep Quality Index (PSQI) and the Horne and Östberg circadian preference questionnaire. We observed that the shift to morningness with increasing age is associated with a significant worsening in sleep quality. We discuss that this sleep profile characterized by morningness and worse sleep quality observed in elderly, when compared to younger people, reflects not necessarily a pathological state, but an expected profile for this age group.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elderly; Sleep quality; Circadian rhythmicity; Chronotype; Sleep ontogeny.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000606

Hyperalgesic effect induced by barbiturates, midazolam and ethanol: pharmacological evidence for GABA-A receptor involvement
Provedor de dados: 56
The involvement of GABA-A receptors in the control of nociception was studied using the tail-flick test in rats. Non-hypnotic doses of the barbiturates phenobarbital (5-50 mg/kg), pentobarbital (17-33 mg/kg), and thiopental (7.5-30 mg/kg), of the benzodiazepine midazolam (10 mg/kg) or of ethanol (0.4-1.6 g/kg) administered by the systemic route reduced the latency for the tail-flick response, thus inducing a 'hyperalgesic' state in the animals. In contrast, non-convulsant doses of the GABA-A antagonist picrotoxin (0.12-1.0 mg/kg) administered systemically induced an increase in the latency for the tail-flick response, therefore characterizing an 'antinociceptive' state. Previous picrotoxin (0.12 mg/kg) treatment abolished the hyperalgesic state induced by...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Barbiturates; Midazolam; Ethanol; Picrotoxin; Hyperalgesia; GABA-A receptor.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200015

Serotonergic neurons in the caudal raphe nuclei discharge in association with activity of masticatory muscles
Provedor de dados: 56
There is a dense serotonergic projection from nucleus raphe pallidus and nucleus raphe obscurus to the trigeminal motor nucleus and serotonin exerts a strong facilitatory action on the trigeminal motoneurons. Some serotonergic neurons in these caudal raphe nuclei increase their discharge during feeding. The objective of the present study was to investigate the possibility that the activity of these serotonergic neurons is related to activity of masticatory muscles. Cats were implanted with microelectrodes and gross electrodes. Caudal raphe single neuron activity, electrocorticographic activity, and splenius, digastric and masseter electromyographic activities were recorded during active behaviors (feeding and grooming), during quiet waking and during...
Palavras-chave: Masticatory muscles; Serotonergic neurons; Caudal raphe nuclei; Feeding behavior; Grooming behavior.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100013

Association between polyclonal B cell activation and the presence of autoantibodies in mice infected with Yersinia enterocolitica O:3
Provedor de dados: 56
Eight-week old conventional female Swiss mice were inoculated intravenously with Yersinia enterocolitica O:3. A second group of normal mice was used as control. Five mice from each group were bled by heart puncture and their spleens were removed for spleen cell collection on the 3rd, 5th, 7th, 10th, 14th and 21st day after infection. Immunoglobulin-secreting spleen cells were detected by the isotype-specific protein A plaque assay. Total immunoglobulin levels were determined in mouse serum by single radial immunodiffusion and the presence of autoantibodies was determined by ELISA. We observed a marked increase in the total number of cells secreting immunoglobulins of all isotypes as early as on the 3rd day post-infection and the peak of secretion occurred...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Yersinia enterocolitica; Polyclonal activation; Experimental infection; Autoantibodies.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300015

Genetic variation of aggrecanase-2 (ADAMTS5) in susceptibility to osteoarthritis
Provedor de dados: 56
Autores: Zhou,Xindie; Jiang,Lifeng; Zhang,Yi; Zhang,Junjie; Zhou,Dong; Wu,Lidong; Huang,Yong; Xu,Nanwei.
Aggrecanase-2 (ADAMTS5) gene is responsible for aggrecan degradation that may contribute to cartilage destruction in a mouse osteoarthritis (OA) model. We aimed to investigate the effects of ADAMTS5 gene polymorphisms on OA risk in a Chinese population. A total of 300 OA patients and 300 controls were recruited and their genotypes for ADAMTS5 gene rs226794 and rs2830585 polymorphisms were determined using a custom-by-design 48-Plex single nucleotide polymorphism Scan™ kit. ADAMTS5-associated genes were identified by co-expression analysis and their functions were investigated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. Bioinformatics analysis showed that ADAMTS5 was significantly related to the components, structural constituent,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ADAMTS5; Polymorphism; Osteoarthritis; Co-expression analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200602

Building bone tissue: matrices and scaffolds in physiology and biotechnology
Provedor de dados: 56
Autores: Riminucci,M.; Bianco,P.
Deposition of bone in physiology involves timed secretion, deposition and removal of a complex array of extracellular matrix proteins which appear in a defined temporal and spatial sequence. Mineralization itself plays a role in dictating and spatially orienting the deposition of matrix. Many aspects of the physiological process are recapitulated in systems of autologous or xenogeneic transplantation of osteogenic precursor cells developed for tissue engineering or modeling. For example, deposition of bone sialoprotein, a member of the small integrin-binding ligand, N-linked glycoprotein family, represents the first step of bone formation in ectopic transplantation systems in vivo. The use of mineralized scaffolds for guiding bone tissue engineering has...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Bone matrix; Bone formation; Biomaterials; Tissue engineering.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800008

Role of 11β-hydroxysteroid dehydrogenase 2 renal activity in potassium homeostasis in rats with chronic renal failure
Provedor de dados: 56
Autores: Yeyati,N.L.; Altuna,M.E.; Damasco,M.C.; Mac Laughlin,M.A.
Aldosterone concentrations vary in advanced chronic renal failure (CRF). The isozyme 11β-hydroxysteroid dehydrogenase 2 (11β-HSD2), which confers aldosterone specificity for mineralocorticoid receptors in distal tubules and collecting ducts, has been reported to be decreased or normal in patients with renal diseases. Our objective was to determine the role of aldosterone and 11β-HSD2 renal microsome activity, normalized for glomerular filtration rate (GFR), in maintaining K+ homeostasis in 5/6 nephrectomized rats. Male Wistar rats weighing 180-220 g at the beginning of the study were used. Rats with experimental CRF obtained by 5/6 nephrectomy (N = 9) and sham rats (N = 10) were maintained for 4 months. Systolic blood pressure and plasma creatinine (Pcr)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aldosterone; 5/6 Nephrectomy; 11β-HSD2; Potassium excretion.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100008

Presurgical ketoprofen, but not morphine, dipyrone, diclofenac or tenoxicam, preempts post-incisional mechanical allodynia in rats
Provedor de dados: 56
Autores: Prado,W.A.; Pontes,R.M.C.
The treatment of pain before it initiates may prevent the persistent pain-induced changes in the central nervous system that amplify pain long after the initial stimulus. The effects of pre- or postoperative intraperitoneal administration of morphine (2 to 8 mg/kg), dipyrone (40 and 80 mg/kg), diclofenac (2 to 8 mg/kg), ketoprofen (10 and 20 mg/kg), and tenoxicam (10 and 20 mg/kg) were studied in a rat model of post-incisional pain. Groups of 5 to 8 male Wistar rats (140-160 g) were used to test each drug dose. An incision was made on the plantar surface of a hind paw and the changes in the withdrawal threshold to mechanical stimulation were evaluated with Von Frey filaments at 1, 2, 6 and 24 h after the surgery. Tenoxicam was given 12 or 6 h...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preemptive analgesia; Morphine; Dipyrone; Diclofenac; Ketoprofen; Tenoxicam.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100016

Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats
Provedor de dados: 56
Follicle-stimulating hormone (FSH) and insulin regulate glycide metabolism in Sertoli cells, thus stimulating lactate production. These stimulatory effects of FSH and insulin do not require protein synthesis, suggesting a modulation of enzyme activity and/or regulation of glucose transport. The present investigation was performed to characterize the hormonal control of lipid metabolism in Sertoli cells. The data indicate that FSH and insulin have a regulatory effect on lipid metabolism in Sertoli cells. After 8 h of preincubation with insulin (5 µg/ml), the activity of the enzyme ATP-citrate lyase in cultured Sertoli cells was increased from 0.19 to 0.34 nmol NAD+ formed µg protein-1 min-1. FSH (100 ng/ml) had no effect on this enzyme. Glycerol phosphate...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sertoli cells; Lipids; FSH; Insulin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500004

Production and characterization of monoclonal antibodies to a Brazilian bovine herpesvirus type 5
Provedor de dados: 56
Autores: Oldoni,I.; Weiblen,R.; Inkelmann,M.A.; Flores,E.F.
Antigens of a bovine herpesvirus type 5 (BHV-5), isolated from a cow with a neurological infection in Rio Grande do Sul State, Brazil, were used to immunize BALB/c mice to produce monoclonal antibodies (mAbs). Eleven hybridomas secreting mAbs directed at BHV-5 antigens were obtained after two fusions and screening of 356 hypoxanthine-aminopterin-thymidine-resistant clones. The mAbs reacted at dilutions up to 1:500 (hybridoma culture supernatant) and up to &gt;1:10,000 (ascitic fluid) in an indirect fluorescent antibody assay (IFA) and in immunoperoxidase staining of BHV-5-infected cells. Four mAbs (1D12, 2E2, 2G10 and 4E4) showed virus-neutralizing activity against the parental BHV-5 isolate. Five mAbs (1F3, 2A6, 2F9, 2G10 and HB24L) reacted in Western...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine herpesvirus type 5 and type 1; BHV-5; BHV-1; Monoclonal antibodies.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200008

Functional expression of kinin B1 and B2 receptors in mouse abdominal aorta
Provedor de dados: 56
Autores: Felipe,S.A.; Rodrigues,E.S.; Martin,R.P.; Paiva,A.C.M.; Pesquero,J.B.; Shimuta,S.I.
Previous studies have shown that the vascular reactivity of the mouse aorta differs substantially from that of the rat aorta in response to several agonists such as angiotensin II, endothelin-1 and isoproterenol. However, no information is available about the agonists bradykinin (BK) and DesArg9BK (DBK). Our aim was to determine the potential expression of kinin B1 and B2 receptors in the abdominal mouse aorta isolated from C57BL/6 mice. Contraction and relaxation responses to BK and DBK were investigated using isometric recordings. The kinins were unable to induce relaxation but concentration-contraction response curves were obtained by applying increasing concentrations of the agonists BK and DBK. These effects were blocked by the antagonists Icatibant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kinin B1 and B2 receptors; Bradykinin; DesArg9bradykinin; Indomethacin; L-NAME.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500007

A semi-automatic computerized method to measure baroreflex-mediated heart rate responses that reduces interobserver variability
Provedor de dados: 56
Autores: Soares,P.P.S.; Ushizima,M.R.; Krieger,E.M.; Irigoyen,M.C.C.
Arterial baroreflex sensitivity estimated by pharmacological impulse stimuli depends on intrinsic signal variability and usually a subjective choice of blood pressure (BP) and heart rate (HR) values. We propose a semi-automatic method to estimate cardiovascular reflex sensitivity to bolus infusions of phenylephrine and nitroprusside. Beat-to-beat BP and HR time series for male Wistar rats (N = 13) were obtained from the digitized signal (sample frequency = 2 kHz) and analyzed by the proposed method (PRM) developed in Matlab language. In the PRM, time series were low-pass filtered with zero-phase distortion (3rd order Butterworth used in the forward and reverse direction) and presented graphically, and parameters were selected interactively. Differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baroreflex sensitivity; Heart rate; Blood pressure; Phenylephrine; Nitroprusside.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600018

Liposomes: from biophysics to the design of peptide vaccines
Provedor de dados: 56
Autores: Frézard,F.
Liposomes (lipid-based vesicles) have been widely studied as drug delivery systems due to their relative safety, their structural versatility concerning size, composition and bilayer fluidity, and their ability to incorporate almost any molecule regardless of its structure. Liposomes are successful in inducing potent in vivo immunity to incorporated antigens and are now being employed in numerous immunization procedures. This is a brief overview of the structural, biophysical and pharmacological properties of liposomes and of the current strategies in the design of liposomes as vaccine delivery systems.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liposome; Immunoadjuvant; Immunostimulant; Vaccine; Encapsulation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200006

Establishment of patient-derived tumor xenograft (PDTX) models using samples from CT-guided percutaneous biopsy
Provedor de dados: 56
Autores: Zhuang,Y.-P.; Zhu,Y.-P.; Wang,H.-Y.; Sun,L.; Zhang,J.; Hao,Y.-P.; Wang,L.
This study aimed to investigate the feasibility of the establishment of a human cancer xenograft model using samples from computed tomography (CT)-guided percutaneous biopsy. Fresh tumor tissues obtained from 10 cancer patients by CT-guided percutaneous biopsy were subcutaneously inoculated into NOD-Prkdcem26Il2rgem26Nju (NCG) mice to establish human patient-derived tumor xenograft (PDTX) models. The formation of first and second generation xenografts was observed, and tumor volume was recorded over time. Tumor tissue consistency between the PDTX model and primary tumors in patients was compared using H&amp;E staining and immunohistochemistry. Pharmacodynamic tests of clinically used chemotherapeutic drugs were conducted on second generation xenografts,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Patient-derived tumor xenografts (PDTX); Computed tomography; X-ray; Percutaneous biopsy; Mouse models.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600705

Effect of age on upper and lower eyelid saccades
Provedor de dados: 56
Autores: Leite,L.V.O.; Cruz,A.A.V.; Messias,A.; Malbouisson,J.M.C.
To study the effect of age on the metrics of upper and lower eyelid saccades, eyelid movement of two groups of 30 subjects each were measured using computed image analysis. The patients were divided on the basis of age into a younger group (20-30 years) and an older group (60-91 years). Eyelid saccade functions were fitted by the damped harmonic oscillator model. Amplitude and peak velocity were used to compare the effect of age on the saccades of the upper and lower eyelid. There was no statistically significant difference in saccade amplitude between groups for the upper eyelid (mean ± SEM; upward, young = 9.18 ± 0.32 mm, older = 8.93 ± 0.31 mm, t = 0.56, P = 0.58; downward, young = 9.11 ± 0.27 mm, older = 8.86 ± 0.32 mm, t = 0.58, P = 0.56) However,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Upper eyelid saccade; Lower eyelid saccade; Aging; Digital image processing.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200017

A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats
Provedor de dados: 56
Autores: Fernandes,A.A.; Faria,T. de O.; Ribeiro Júnior,R.F.; Costa,G.P.; Marchezini,B.; Silveira,E.A.; Angeli,J.K.; Stefanon,I.; Vassallo,D.V.; Lizardo,J.H.
Resistance training evokes myocardial adaptation; however, the effects of a single resistance exercise session on cardiac performance are poorly understood or investigated. This study aimed to investigate the effects of a single resistance exercise session on the myocardial contractility of spontaneously hypertensive rats (SHRs). Male 3-month-old SHRs were divided into two groups: control (Ct) and exercise (Ex). Control animals were submitted to sham exercise. Blood pressure was measured in conscious rats before the exercise session to confirm the presence of arterial hypertension. Ten minutes after the exercise session, the animals were anesthetized and killed, and the hearts were removed. Cardiac contractility was evaluated in the whole heart by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac; Contractility; Myocardium; Proteins; Resistance exercise.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900813

Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model
Provedor de dados: 56
Autores: Li,Lin; Zou,Changlin; Zhou,Zhenli; Wang,Ximo; Yu,Xiangyang.
The objective was to study the effect of mechanical intestinal obstruction in rats on the phenotype of interstitial cells of Cajal (ICC). Healthy Wistar rats were randomly divided into sham-operation group (C), one day obstruction group (M1), two days obstruction group (M2), and three days obstruction group (M3), with 10 rats in each group. The expression of SCF mRNA and c-Kit protein in intestinal tissue was investigated by RT-PCR and immunohistochemistry. Compared with the sham-operation group, the relative expression of SCF mRNA and the expression of c-Kit protein in intestinal tissue were significantly decreased in both obstruction groups. Levels decreased gradually with the prolongation of obstruction time, and significantly decreased on the 3rd day...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal obstruction; Interstitial cells of Cajal; Stem cell factor; C-Kit protein.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000611

Humoral response to low molecular weight antigens of Mycobacterium tuberculosis by tuberculosis patients and contacts
Provedor de dados: 56
Autores: Beck,S.T.; Leite,O.M.; Arruda,R.S.; Ferreira,A.W.
Much effort has been devoted to the identification of immunologically important antigens of Mycobacterium tuberculosis and to the combination of target antigens to which antibodies from serum of tuberculous patients could react specifically. We searched for IgG antibodies specific for antigens of 45 to 6 kDa obtained after sonication of the well-characterized wild M. tuberculosis strain in order to detect differences in the antibody response to low molecular weight antigens from M. tuberculosis between patients with pulmonary tuberculosis and contacts. Specific IgG antibodies for these antigens were detected by Western blot analysis of 153 serum samples collected from 51 patients with confirmed pulmonary tuberculosis. Three samples were collected from each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Western blot; Profile; Tuberculosis; Antigens.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400013

Role of gelatinases MMP-2 and MMP-9 in tissue remodeling following acute lung injury
Provedor de dados: 56
Autores: Corbel,M.; Boichot,E.; Lagente,V.
Acute lung injury is characterized by a severe disruption of alveolo-capillary structures and includes a variety of changes in lung cell populations. Evidence suggests the occurrence of rupture of the basement membranes and interstitial matrix remodeling during acute lung injury. The dynamic equilibrium of the extracellular matrix (ECM) under physiological conditions is a consequence of the balance between the regulation of synthesis and degradation of ECM components. Matrix metalloproteinases (MMPs) represent a group of enzymes involved in the degradation of most of the components of the ECM and therefore participate in tissue remodeling associated with pathological situations such as acute lung injury. MMP activity is regulated by proteolytic activation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Matrix metalloproteinase; TIMPs; Acute lung injury; ARDS; LPS; Extracellular matrix.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700004

Unconventional neurotransmitters, neurodegeneration and neuroprotection
Provedor de dados: 56
Autores: Leonelli,M.; Torrão,A.S.; Britto,L.R.G.
Neurotransmitters are also involved in functions other than conventional signal transfer between nerve cells, such as development, plasticity, neurodegeneration, and neuroprotection. For example, there is a considerable amount of data indicating developmental roles for the glutamatergic, cholinergic, dopaminergic, GABA-ergic, and ATP/adenosine systems. In this review, we discuss the existing literature on these "new" functions of neurotransmitters in relation to some unconventional neurotransmitters, such as the endocannabinoids and nitric oxide. Data indicating both transcriptional and post-transcriptional modulation of endocannabinoid and nitrinergic systems after neural lesions are discussed in relation to the non-conventional roles of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endocannabinoids; Cannabinoid receptors; Vanilloid receptors; Nitric oxide.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100011

Nitric oxide synthesis and biological functions of nitric oxide released from ruthenium compounds
Provedor de dados: 56
Autores: Pereira,A.C.; Paulo,M.; Araújo,A.V.; Rodrigues,G.J.; Bendhack,L.M.
During three decades, an enormous number of studies have demonstrated the critical role of nitric oxide (NO) as a second messenger engaged in the activation of many systems including vascular smooth muscle relaxation. The underlying cellular mechanisms involved in vasodilatation are essentially due to soluble guanylyl-cyclase (sGC) modulation in the cytoplasm of vascular smooth cells. sGC activation culminates in cyclic GMP (cGMP) production, which in turn leads to protein kinase G (PKG) activation. NO binds to the sGC heme moiety, thereby activating this enzyme. Activation of the NO-sGC-cGMP-PKG pathway entails Ca2+ signaling reduction and vasodilatation. Endothelium dysfunction leads to decreased production or bioavailability of endogenous NO that could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Vasodilatation; Ruthenium complex; NO-synthase; Resistance artery; Conduit vessel.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900017

HEV, TTV and GBV-C/HGV markers in patients with acute viral hepatitis
Provedor de dados: 56
Autores: Lyra,A.C.; Pinho,J.R.R.; Silva,L.K.; Sousa,L.; Saraceni,C.P.; Braga,E.L.; Pereira,J.E.; Zarife,M.A.S.; Reis,M.G.; Lyra,L.G.C.; Silva,L.C. da; Carrilho,F.J.
The aim of the present study was to evaluate the prevalence of HEV, TTV and GBV-C/GBV-C/HGV in patients with acute viral hepatitis A, B and non-A-C. We evaluated sera of 94 patients from a sentinel program who had acute hepatitis A (N = 40), B (N = 42) and non-A-C (N = 12); 71 blood donors served as controls. IgM and anti-HEV IgG antibodies were detected by enzyme immunoassay using commercial kits. TTV and GBV-C/HGV were detected by nested PCR; genotyping was done by sequencing and phylogenetic analysis. Anti-HEV IgG was present in 38, 10 and 17% of patients with hepatitis A, B and non-A-C. Four patients with hepatitis A and 1 with non-A-C hepatitis also had anti-HEV IgM detected in serum. TTV was detected in 21% of patients with acute hepatitis and in 31%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute hepatitis; Hepatitis E; TTV; GBV-C/HGV; Genotype; Brazil.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500015

Gene expression of estrogen and oxytocin receptors in the uterus of pregnant and parturient bitches
Provedor de dados: 56
Autores: Veiga,G.A.L.; Milazzotto,M.P.; Nichi,M.; Lúcio,C.F.; Silva,L.C.G.; Angrimani,D.S.R.; Vannucchi,C.I.
In the canine species, the precise mechanisms of pregnancy maintenance and the initiation of parturition are not completely understood. The expression of genes encoding the receptors for estrogen (ERα mRNA) and oxytocin (OTR mRNA) was studied in the endometrium and myometrium during pregnancy and parturition in dogs. Real-time PCR was performed to quantify the levels of ERα mRNA and OTR mRNA in the uterus of bitches during early (up to 20 days of gestation), mid (20 to 40 days) and late pregnancy (41 to 60 days), and parturition (first stage of labor). All tissues expressed ERα and OTR mRNA, and are thus possibly able to respond to eventual estrogen and oxytocin hormonal stimuli. No statistically significant differences in the expression of ERα mRNA were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine; Estrogen; Oxytocin; Receptors; Pregnancy; Parturition.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400339

Differential behavioral outcomes of 3,4-methylenedioxymethamphetamine (MDMA-ecstasy) in anxiety-like responses in mice
Provedor de dados: 56
Autores: Ferraz-de-Paula,V.; Stankevicius,D.; Ribeiro,A.; Pinheiro,M.L.; Rodrigues-Costa,E.C.; Florio,J.C.; Lapachinske,S.F.; Moreau,R.L.M.; Palermo-Neto,J.
Anxiolytic and anxiogenic-like behavioral outcomes have been reported for methylenedioxymethamphetamine (MDMA or ecstasy) in rodents. In the present experiment, we attempted to identify behavioral, hormonal and neurochemical outcomes of MDMA treatment to clarify its effects on anxiety-related responses in 2-month-old Balb/c male mice (25-35 g; N = 7-10 mice/group). The behavioral tests used were open field, elevated plus maze, hole board, and defensive behavior against predator odor. Moreover, we also determined striatal dopamine and dopamine turnover, and serum corticosterone levels. MDMA was injected ip at 0.2, 1.0, 5.0, 8.0, 10, or 20 mg/kg. MDMA at 10 mg/kg induced the following significant (P &lt; 0.05) effects: a) a dose-dependent increase in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corticosterone; Open field; Plus maze; Hole board; Predator odor test; Ecstasy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500008

Random-start controlled ovarian stimulation for emergency fertility preservation in a patient with myelodysplastic syndrome: a case report
Provedor de dados: 56
Autores: Cai,H.; Shen,H.
This study reports a case of a gonadotropin-releasing hormone agonist trigger in a young female with myelodysplastic syndrome (MDS) who underwent fertility preservation using random-start controlled ovarian stimulation. This method involves the stimulation of the ovary regardless of a patient's menstrual-cycle phase. A review of the related literature is also provided. A 17-year-old patient was diagnosed with MDS and required initiation of peripheral blood stem cell transplantation within a maximum of 3 weeks and was in the luteal phase of the menstrual cycle when the possibility of attempting preservation of fertility was presented to her. She opted for a random-start controlled ovarian stimulation with gonadotropins. With successful hemorrhagic...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Emergency fertility preservation; Random-start; Ovarian stimulation; Myelodysplastic syndrome.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600501

Role of endogenous glucocorticoids in hyperalgesia and edema in old arthritic rats
Provedor de dados: 56
Autores: Yokoro,C.M.; Tatsuo,M.A.K.F.; Pereira,L.S.M.; Alves,D.L.F.; Francischi,J.N.
We compared the intensity and frequency of arthritis in old (8-12 months, N = 12) and juvenile (2 months, N = 10) rats and determined the role played by adrenal glands in this disorder. Arthritis was induced by subcutaneous injection of Mycobacterium butyricum at the base of the tail of female Holtzman rats at day zero. Paw edema and hyperalgesia were monitored from day zero to day 21 after induction as signs of arthritis development. Some (N = 11) old animals were adrenalectomized bilaterally and treated with dexamethasone or celecoxib immediately following surgery. All bilaterally adrenalectomized old animals became susceptible to arthritis and the onset of disease was shortened from the 10th to the 5th day. Hyperalgesia and paw edema responses were less...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperalgesia; Edema; Adjuvant arthritis; Adrenalectomy; Dexamethasone; Old rats.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100011

Acute extracellular fluid volume changes increase ileocolonic resistance to saline flow in anesthetized dogs
Provedor de dados: 56
Autores: Santiago Jr.,A.T.; Gondim,F. de-A.A.; Cavalcante,D.I.M.; da-Graça,J.R.V.; de-Oliveira,G.R.; dos-Santos,A.A.; Rola,F.H.
We determined the effect of acute extracellular fluid volume changes on saline flow through 4 gut segments (ileocolonic, ileal, ileocolonic sphincter and proximal colon), perfused at constant pressure in anesthetized dogs. Two different experimental protocols were used: hypervolemia (iv saline infusion, 0.9% NaCl, 20 ml/min, volume up to 5% body weight) and controlled hemorrhage (up to a 50% drop in mean arterial pressure). Mean ileocolonic flow (N = 6) was gradually and significantly decreased during the expansion (17.1%, P&lt;0.05) and expanded (44.9%, P&lt;0.05) periods while mean ileal flow (N = 7) was significantly decreased only during the expanded period (38%, P&lt;0.05). Mean colonic flow (N = 7) was decreased during expansion (12%, P&lt;0.05) but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ileocolonic motility; Extracellular fluid volume; Expansion; Hemorrhage; Atropine.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800014

Dorsal periaqueductal gray stimulation facilitates anxiety-, but not panic-related, defensive responses in rats tested in the elevated T-maze
Provedor de dados: 56
Autores: Camplesi Jr,M.; Bortoli,V.C.de; Soares,V. de Paula; Nogueira,R.L.; Zangrossi Jr.,H.
The escape response to electrical or chemical stimulation of the dorsal periaqueductal gray matter (DPAG) has been associated with panic attacks. In order to explore the validity of the DPAG stimulation model for the study of panic disorder, we determined if the aversive consequences of the electrical or chemical stimulation of this midbrain area can be detected subsequently in the elevated T-maze. This animal model, derived from the elevated plus-maze, permits the measurement in the same rat of a generalized anxiety- and a panic-related defensive response, i.e., inhibitory avoidance and escape, respectively. Facilitation of inhibitory avoidance, suggesting an anxiogenic effect, was detected in male Wistar rats (200-220 g) tested in the elevated T-maze 30...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Panic disorder; Anxiety; Elevated T-maze; Dorsal periaqueductal gray stimulation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100005

Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis
Provedor de dados: 56
Autores: Mendonça,D.M.F.; Chimelli,L.; Martinez,A.M.B.
Amyotrophic lateral sclerosis (ALS), a neurodegenerative disease of unknown etiology, affects motor neurons leading to atrophy of skeletal muscles, paralysis and death. There is evidence for the accumulation of neurofilaments (NF) in motor neurons of the spinal cord in ALS cases. NF are major structural elements of the neuronal cytoskeleton. They play an important role in cell architecture and differentiation and in the determination and maintenance of fiber caliber. They are composed of three different polypeptides: light (NF-L), medium (NF-M) and heavy (NF-H) subunits. In the present study, we performed a morphological and quantitative immunohistochemical analysis to evaluate the accumulation of NF and the presence of each subunit in control and ALS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amyotrophic lateral sclerosis; Spheroids; Neurofilament proteins; Immunohistochemistry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600015

Detection of early apoptosis and cell death in T CD4+ and CD8+ cells from lesions of patients with localized cutaneous leishmaniasis
Provedor de dados: 56
Autores: Bertho,A.L.; Santiago,M.A.; Da-Cruz,A.M.; Coutinho,S.G.
Human localized cutaneous leishmaniasis (LCL), induced by Leishmania braziliensis, ranges from a clinically mild, self-healing disease with localized cutaneous lesions to severe forms which can present secondary metastatic lesions. The T cell-mediated immune response is extremely important to define the outcome of the disease; however, the underlying mechanisms involved are not fully understood. A flow cytometric analysis of incorporation of 7-amino actinomycin D and CD4+ or CD8+ T cell surface phenotyping was used to determine whether different frequencies of early apoptosis or accidental cell death occur at different stages of LCL lesions. When all cells obtained from a biopsy sample were analyzed, larger numbers of early apoptotic and dead cells were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Flow cytometry; Human leishmaniasis; Cell death; T cells.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300010

Aberrant crypt foci and colon cancer: comparison between a short- and medium-term bioassay for colon carcinogenesis using dimethylhydrazine in Wistar rats
Provedor de dados: 56
Autores: Rodrigues,M.A.M.; Silva,L.A.G.; Salvadori,D.M.F.; de Camargo,J.L.V.; Montenegro,M.R.
Aberrant crypt foci (ACF) in the colon of carcinogen-treated rodents are considered to be the earliest hallmark of colon carcinogenesis. In the present study the relationship between a short-term (4 weeks) and medium-term (30 weeks) assay was assessed in a model of colon carcinogenesis induced by dimethylhydrazine (DMH) in the rat. Six-week-old male Wistar rats were given subcutaneous injections of DMH (40 mg/kg) twice a week for 2 weeks and killed at the end of the 4th or 30th week. ACF were scored for number, distribution pattern along the colon and crypt multiplicity in 0.1% methylene-blue whole-mount preparations. ACF were distinguished from normal crypts by their larger size and elliptical shape. The incidence, distribution and morphology of colon...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aberrant crypt foci; Preneoplastic lesion; Colon carcinogenesis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300010

Delta sleep instability in children with chronic arthritis
Provedor de dados: 56
Autores: Lopes,M.C.; Guilleminault,C.; Rosa,A.; Passarelli,C.; Roizenblatt,S.; Tufik,S.
The objective of the present study was to evaluate the expression of a cyclic alternating pattern (CAP) in slow wave sleep (SWS) in children with the well-defined chronic syndrome juvenile idiopathic arthritis (JIA). Twelve patients (9-17 years of age), 7 girls, with JIA were compared to matched controls by age, pubertal stage and gender. After one night of habituation in the sleep laboratory, sleep measurements were obtained by standard polysomnography with conventional sleep scoring and additional CAP analyses. The sleep parameters of the JIA and control groups were similar for sleep efficiency (91.1 ± 6.7 vs 95.8 ± 4.0), sleep stage in minutes: stage 1 (16.8 ± 8.5 vs 17.8 ± 4.0), stage 2 (251.9 ± 41 vs 262.8 ± 38.1), stage 3 (17.0 ± 6.0 vs 15.1 ± 5.7),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Children; Chronic-arthritis; Cyclic alternating pattern; Sleep fragmentation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000018

Reduced pancreatic β-cell mass is associated with decreased FoxO1 and Erk1/2 protein phosphorylation in low-protein malnourished rats
Provedor de dados: 56
Autores: Rafacho,A.; Giozzet,V.A.G.; Boschero,A.C.; Abrantes,J.L.F.; Cestari,T.M.; Carneiro,E.M.; Bosqueiro,J.R.
A low-protein diet leads to functional and structural pancreatic islet alterations, including islet hypotrophy. Insulin-signaling pathways are involved in several adaptive responses by pancreatic islets. We determined the levels of some insulin-signaling proteins related to pancreatic islet function and growth in malnourished rats. Adult male Wistar rats (N = 20 per group) were fed a 17% protein (normal-protein diet; NP) or 6% protein (low-protein diet; LP), for 8 weeks. At the end of this period, blood glucose and serum insulin and albumin levels were measured. The morphometric parameters of the endocrine pancreas and the content of some proteins in islet lysates were determined. The β-cell mass was significantly reduced (≅65%) in normoglycemic but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-cell mass; Insulin signaling; Low-protein diet; Pancreatic islets; Protein phosphorylation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000010

Effects of estragole on the compound action potential of the rat sciatic nerve
Provedor de dados: 56
Autores: Leal-Cardoso,J.H.; Matos-Brito,B.G.; Lopes-Junior,J.E.G.; Viana-Cardoso,K.V.; Sampaio-Freitas,A.B.; Brasil,R.O.; Coelho-de-Souza,A.N.; Albuquerque,A.A.C.
Estragole, a relatively nontoxic terpenoid ether, is an important constituent of many essential oils with widespread applications in folk medicine and aromatherapy and known to have potent local anesthetic activity. We investigated the effects of estragole on the compound action potential (CAP) of the rat sciatic nerve. The experiments were carried out on sciatic nerves dissected from Wistar rats. Nerves, mounted in a moist chamber, were stimulated at a frequency of 0.2 Hz, with electric pulses of 50-100-µs duration at 10-20 V, and evoked CAP were monitored on an oscilloscope and recorded on a computer. CAP control parameters were: peak-to-peak amplitude (PPA), 9.9 ± 0.55 mV (N = 15), conduction velocity, 92.2 ± 4.36 m/s (N = 15), chronaxy, 45.6 ± 3.74 µs...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Estragole; Essential oil; Sciatic nerve; Compound action potential; Nerve conduction velocity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800009

A pulsatile flow model for in vitro quantitative evaluation of prosthetic valve regurgitation
Provedor de dados: 56
Autores: Giuliatti,S.; Gallo Jr.,L.; Almeida-Filho,O.C.; Schmidt,A.; Marin-Neto,J.A.; Pelá,C.A.; Maciel,B.C.
A pulsatile pressure-flow model was developed for in vitro quantitative color Doppler flow mapping studies of valvular regurgitation. The flow through the system was generated by a piston which was driven by stepper motors controlled by a computer. The piston was connected to acrylic chambers designed to simulate "ventricular" and "atrial" heart chambers. Inside the "ventricular" chamber, a prosthetic heart valve was placed at the inflow connection with the "atrial" chamber while another prosthetic valve was positioned at the outflow connection with flexible tubes, elastic balloons and a reservoir arranged to mimic the peripheral circulation. The flow model was filled with a 0.25% corn starch/water suspension to improve Doppler imaging. A continuous flow...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Flow model; Echocardiography; Valvular regurgitation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300013

Cell lineage relationship in the stomach of normal and genetically manipulated mice
Provedor de dados: 56
Autores: Karam,S.M.
The oxyntic mucosa of the mouse stomach is lined with a heterogeneous population of cells that form numerous short pits continuous with long tubular glands. Tritiated thymidine radioautography has made it possible to pinpoint the origin of all cell types and to follow the differentiation/migration of different cell lineages along the pit-gland unit. The proliferating multipotent stem cells functionally anchored in the upper glandular region, the isthmus, give rise to three main lineage precursors: 1) pre-pit cells, which migrate upward to the pit while differentiating into mucus-producing pit cells; 2) pre-neck cells, which migrate downward to the glandular neck while differentiating into mucus-producing neck cells that, by approaching the glandular base,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radioautography; Gastric epithelium; Stem cells; Cell proliferation/differentiation programs; Lineage progenitors; Transgenic mice.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200010

Low vaspin levels are related to endothelial dysfunction in patients with ankylosing spondylitis
Provedor de dados: 56
Autores: Wang,H.H.; Wang,Q.F.
Vaspin is a novel adipocytokine associated with glucose tolerance and chronic inflammation. Some studies reveal that vaspin may be involved in cardiovascular diseases. Our objective was to investigate the relationship between serum vaspin levels and endothelial function in patients with ankylosing spondylitis. One hundred and twenty patients with newly diagnosed ankylosing spondylitis and 100 healthy subjects were studied. Serum vaspin levels were measured with enzyme-linked immunosorbent assay. High resolution ultrasound was used to measure brachial artery diameter at rest, after reactive hyperemia (flow-mediated dilation, FMD) and after sublingual glyceryltrinitrate. Serum vaspin level in patients was 1.92±1.03 ng/mL, which was significantly lower than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaspin; Endothelial function; Ankylosing spondylitis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700705

Inhibition of PKC-dependent extracellular Ca2+ entry contributes to the depression of contractile activity in long-term pressure-overloaded endothelium-denuded rat aortas
Provedor de dados: 56
Autores: Padilla,J.; López,R.M.; López,P.; Castillo,M.C.; Querejeta,E.; Ruiz,A.; Castillo,E.F.
We examined the contractile responsiveness of rat thoracic aortas under pressure overload after long-term suprarenal abdominal aortic coarctation (lt-Srac). Endothelium-dependent angiotensin II (ANG II) type 2 receptor (AT2R)-mediated depression of contractions to ANG II has been reported in short-term (1 week) pressure-overloaded rat aortas. Contractility was evaluated in the aortic rings of rats subjected to lt-Srac or sham surgery (Sham) for 8 weeks. ANG I and II levels and AT2R protein expression in the aortas of lt-Srac and Sham rats were also evaluated. lt-Srac attenuated the contractions of ANG II and phenylephrine in the aortas in an endothelium-independent manner. However, lt-Srac did not influence the transient contractions induced in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Long-term pressure overload; Rat aorta; Protein kinase C.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900789

Neutralizing capacity of a new monovalent anti-Bothrops atrox antivenom: comparison with two commercial antivenoms
Provedor de dados: 56
Three horse-derived antivenoms were tested for their ability to neutralize lethal, hemorrhagic, edema-forming, defibrinating and myotoxic activities induced by the venom of Bothrops atrox from Antioquia and Chocó (Colombia). The following antivenoms were used: a) polyvalent (crotaline) antivenom produced by Instituto Clodomiro Picado (Costa Rica), b) monovalent antibothropic antivenom produced by Instituto Nacional de Salud-INS (Bogotá), and c) a new monovalent anti-B. atrox antivenom produced with the venom of B. atrox from Antioquia and Chocó. The three antivenoms neutralized all toxic activities tested albeit with different potencies. The new monovalent anti-B. atrox antivenom showed the highest neutralizing ability against edema-forming and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bothrops atrox; Snake venom; Antivenom; Neutralization; Antioquia; Chocó.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300011

The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission
Provedor de dados: 56
Autores: Borja-Oliveira,C.R.; Durigon,A.M.; Vallin,A.C.C.; Toyama,M.H.; Souccar,C.; Marangoni,S.; Rodrigues-Simioni,L.
The neuromuscular effects of Bothrops neuwiedii pauloensis (jararaca-pintada) venom were studied on isolated chick biventer cervicis nerve-muscle preparations. Venom concentrations of 5-50 µg/ml produced an initial inhibition and a secondary increase of indirectly evoked twitches followed by a progressive concentration-dependent and irreversible neuromuscular blockade. At venom concentrations of 1-20 µg/ml, the responses to 13.4 mM KCl were inhibited whereas those to 110 µM acetylcholine alone and cumulative concentrations of 1 µM to 10 mM were unaffected. At venom concentrations higher than 50 µg/ml, there was pronounced muscle contracture with inhibition of the responses to acetylcholine, KCl and direct stimulation. At 20-24ºC, the venom (50 µg/ml)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chick biventer cervicis; Myotoxicity; Neurotoxicity; Phospholipase A2; Presynaptic action.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500009

Berberine induces apoptosis via ROS generation in PANC-1 and MIA-PaCa2 pancreatic cell lines
Provedor de dados: 56
Autores: Park,S.H.; Sung,J.H.; Kim,E.J.; Chung,N.
Pancreatic cancer is the fourth leading cause of cancer death. Gemcitabine is widely used as a chemotherapeutic agent for the treatment of pancreatic cancer, but the prognosis is still poor. Berberine, an isoquinoline alkaloid extracted from a variety of natural herbs, possesses a variety of pharmacological properties including anticancer effects. In this study, we investigated the anticancer effects of berberine and compared its use with that of gemcitabine in the pancreatic cancer cell lines PANC-1 and MIA-PaCa2. Berberine inhibited cell growth in a dose-dependent manner by inducing cell cycle arrest and apoptosis. After berberine treatment, the G1 phase of PANC-1 cells increased by 10% compared to control cells, and the G1 phase of MIA-PaCa2 cells was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pancreatic cancer; Berberine; Gemcitabine; Apoptosis; Cell cycle arrest.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200111

Serotyping HIV-1 with V3 peptides: detection of high avidity antibodies presenting clade-specific reactivity
Provedor de dados: 56
Autores: Casseb,J.; Katzenstein,D.; Winters,M.; Brigido,L.F.M.; Duarte,A.J.S.; Hendry,R.M.
The main objective of the present study was to assess the specificity and sensitivity of a modified assay using short synthetic peptides of the V3 region of HIV-1 gp120, which is the main target for neutralizing antibodies. Results from an enzyme immunoassay (EIA) employing a panel of synthetic peptides of HIV-1 subtypes and using urea washes to detect high avidity antibodies (AAV3) were compared with those obtained by the heteroduplex mobility assay and DNA sequencing. The EIA correctly typed 100% of subtype B (sensitivity = 1.0; specificity = 0.95), 100% of HIV-1 E samples (sensitivity = 1.0; specificity = 1.0), and 95% of subtype C specimens (sensitivity = 0.95; specificity = 0.94). In contrast, only 50% of subtype A (sensitivity = 0.5; specificity =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; Viral diversity; Serotypes; V3 serology.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300013

Collagen XVIII/endostatin expression in experimental endotoxemic acute renal failure
Provedor de dados: 56
Autores: Cichy,M.C.; Rocha,F.G.G.; Tristão,V.R.; Pessoa,E.A.; Cenedeze,M.A.; Nürmberg Junior,R.; Schor,N.; Bellini,M.H.
Acute renal failure (ARF) is a frequent complication of Gram-negative sepsis, with a high risk of mortality. Lipopolysaccharide (LPS)-induced ARF is associated with hemodynamic changes that are strongly influenced by the overproduction of nitric oxide (NO) through the cytokine-mediated up-regulation of inducible NO synthase. LPS-induced reductions in systemic vascular resistance paradoxically culminate in renal vasoconstriction. Collagen XVIII is an important component of the extracellular matrix expressed in basement membranes. Its degradation by matrix metalloproteases, cathepsins and elastases results in the formation of endostatin, claimed to have antiangiogenic activity and to be a prominent vasorelaxing agent. We evaluated the expression of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute renal failure; Lipopolysaccharide; Gene expression; Endostatin; CD31.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200005

Clinical and immunological features of patients with atopy and concomitant HTLV-1 infection
Provedor de dados: 56
Autores: Gaspar-Sobrinho,F.P.; Souza-Machado,A.; Santos,S.B.; Orge,G.; Lessa,H.A.; Cruz,A.A.; Carvalho,E.M.
Human T-cell lymphotropic virus type 1 (HTLV-1) induces an exacerbated type 1 immune response characterized by high spontaneous IFN-γ and TNF-α production. Allergic rhinitis and asthma are associated with the type 2 immune response, with elevated secretion of IL-4 and IL-5. The aim of this study was to characterize the immune response in atopic HTLV-1 carriers. The cytokine profile of atopic HTLV-1 carriers (N = 10; all females) was compared with that of non-atopic HTLV-1 carriers (N = 14; 9 females and 5 males). Mean patient age of atopic and non-atopic groups was 45 ± 8 and 38 ± 11 years, respectively. All atopic HTLV-1 carriers had rhinitis with or without asthma and a skin prick test positive for Dermatophagoides pteronyssinus antigen 1 (Derp-1). There...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Atopy; Cytokines; HTLV-1; Rhinitis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200005

Effect of salt intake and potassium supplementation on brachial-ankle pulse wave velocity in Chinese subjects: an interventional study
Provedor de dados: 56
Autores: Wang,Y.; Mu,J.J.; Geng,L.K.; Wang,D.; Ren,K.Y.; Guo,T.S.; Chu,C.; Xie,B.Q.; Liu,F.Q.; Yuan,Z.Y.
Accumulating evidence has suggested that high salt and potassium might be associated with vascular function. The aim of this study was to investigate the effect of salt intake and potassium supplementation on brachial-ankle pulse wave velocity (PWV) in Chinese subjects. Forty-nine subjects (28-65 years of age) were selected from a rural community of northern China. All subjects were sequentially maintained on a low-salt diet for 7 days (3.0 g/day NaCl), a high-salt diet for an additional 7 days (18.0 g/day NaCl), and a high-salt diet with potassium supplementation for a final 7 days (18.0 g/day NaCl+4.5 g/day KCl). Brachial-ankle PWV was measured at baseline and on the last day of each intervention. Blood pressure levels were significantly increased from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulse wave velocity; Salt; Potassium; Interventional study.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100083

In vivo and in vitro anti-inflammatory and anti-nociceptive activities of lovastatin in rodents
Provedor de dados: 56
Autores: Gonçalves,D.O.; Calou,I.B.F.; Siqueira,R.P.; Lopes,A.A.; Leal,L.K.A.; Brito,G.A.C.; Tomé,A.R.; Viana,G.S.B.
Statins are among the most prescribed drugs in recent clinical practice. They are also known for their pleiotropic actions, which are independent of their lipid-lowering properties. The effect of lovastatin was investigated against carrageenan-induced paw edema in male Wistar rats (200-250 g) and on leukocyte migration, as measured by carrageenan-induced peritonitis in male Swiss mice (20-25 g), which are models of acute inflammation. Lovastatin (administered 1 h prior to carrageenan), at oral doses of 2, 5, and 10 mg/kg, markedly attenuated paw edema formation in rats at the 4th hour after carrageenan injection (25, 43, and 37% inhibition, respectively). Inhibitions of 20, 45 and 80% were observed in the leukocyte migration, as evaluated by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Statin; Inflammation; Anti-nociception; Myeloperoxidase; Cytokines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200011

Umbilical cord blood and neonatal endothelin-1 levels in preterm newborns with and without respiratory distress syndrome
Provedor de dados: 56
Autores: Benjamin,A.C.W.; Silveira,R.C.; Procianoy,R.S.
Increased pulmonary vascular resistance in preterm newborn infants with respiratory distress syndrome is suggested, and endothelin-1 plays an important role in pulmonary vascular reactivity in newborns. We determined umbilical cord blood and neonatal (second sample) levels of endothelin-1 in 18 preterm newborns with respiratory distress syndrome who had no clinical or echocardiographic diagnosis of pulmonary hypertension and 22 without respiratory distress syndrome (gestational ages: 31.4 ± 1.6 and 29.3 ± 2.3 weeks, respectively). Umbilical cord blood and a second blood sample taken 18 to 40 h after birth were used for endothelin-1 determination by enzyme immunoassay. Median umbilical cord blood endothelin-1 levels were similar in both groups (control:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelin-1; Respiratory distress syndrome; Prematurity; Hyaline membrane disease; Pulmonary hypertension; SNAPPE II.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900017

ApoE polymorphisms and diarrheal outcomes in Brazilian shanty town children
Provedor de dados: 56
Autores: Oriá,R.B.; Patrick,P.D.; Oriá,M.O.B.; Lorntz,B.; Thompson,M.R.; Azevedo,O.G.R.; Lobo,R.N.B.; Pinkerton,R.F.; Guerrant,R.L.; Lima,A.A.M.
A series of studies have shown that the heavy burdens of diarrheal diseases in the first 2 formative years of life in children living in urban shanty towns have negative effects on physical and cognitive development lasting into later childhood. We have shown that APOE4 is relatively common in shanty town children living in Brazil (13.4%) and suggest that APOE4 has a protective role in cognitive development as well as weight-for-height in children with heavy burdens of diarrhea in early childhood (64/123; 52%), despite being a marker for cognitive decline with Alzheimer’s and cardiovascular diseases later in life. APOE2 frequency was higher among children with heaviest diarrhea burdens during the first 2 years of life, as detected by PCR using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein E; Malnutrition; Diarrhea; Children.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300005

Structural properties of lipid reconstructs and lipid composition of normotensive and hypertensive rat vascular smooth muscle cell membranes
Provedor de dados: 56
Autores: Oliveira,T.R.; Lamy,M.T.; De Paula,U.M.; Guimarães,L.L.; Toledo,M.S.; Takahashi,H.K.; Straus,A.H.; Lindsey,C.J.; Paiva,T.B.
Multiple cell membrane alterations have been reported to be the cause of various forms of hypertension. The present study focuses on the lipid portion of the membranes, characterizing the microviscosity of membranes reconstituted with lipids extracted from the aorta and mesenteric arteries of spontaneously hypertensive (SHR) and normotensive control rat strains (WKY and NWR). Membrane-incorporated phospholipid spin labels were used to monitor the bilayer structure at different depths. The packing of lipids extracted from both aorta and mesenteric arteries of normotensive and hypertensive rats was similar. Lipid extract analysis showed similar phospholipid composition for all membranes. However, cholesterol content was lower in SHR arteries than in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Smooth muscle cell lipids; Membrane microviscosity; Spontaneously hypertensive rats; Lipid composition; Spin label; Electron spin resonance.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900012

Phytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicles
Provedor de dados: 56
Autores: Cunha,E.V.; Costa,J.J.N.; Rossi,R.O.D.S.; Silva,A.W.B.; Passos,J.R.S.; Portela,A.M.L.R.; Pereira,D.C.S.T.; Donato,M.A.M.; Campello,C.C.; Saraiva,M.V.A.; Peixoto,C.A.; Silva,J.R.V.; Santos,R.P.
The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles culturedin vitro. Secondary follicles (∼0.2 mm) were isolated from the cortex of caprine ovaries and cultured individually for 6 days in α-MEM+ supplemented with PHA (0, 1, 10, 50, 100, or 200 µg/mL). After 6 days of culture, follicle diameter and survival, antrum formation, ultrastructure and expression of mRNA for FSH receptors (FSH-R), proliferating cell nuclear antigen (PCNA), and neuronal nitric oxide synthase were determined. All treatments maintained follicular survival [α-MEM+ (94.59%); 1 µg/mL PHA (96.43%); 10 µg/mL PHA (84.85%); 50 µg/mL PHA (85.29%); 100 µg/mL PHA (88.57%), and 200 µg/mL PHA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caprine; Antrum formation; FSH-R; PCNA; Ultrastructure.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300245

Oridonin induces growth inhibition and apoptosis in human gastric carcinoma cells by enhancement of p53 expression and function
Provedor de dados: 56
Autores: Bi,Enxu; Liu,Dengqiang; Li,Youxi; Mao,Xuying; Wang,Aihua; Wang,Jingtao.
The tumor suppressive role of oridonin, an active compound extracted from Rabdosia rubescens, has been proven in several gastric cancer (GC) cell lines. The present study aimed to evaluate the effect of oridonin on another GC cell line, SNU-216, and explore the potential mechanisms. The viable cell numbers, cell migration, survival fraction, and cell viability were, respectively, evaluated by trypan blue exclusion assay, wound healing assay, clonogenic assay, and CCK-8 assay. Cell apoptosis was determined by flow cytometry assay and western blot. The expression of p53 was inhibited by transient transfection, and the efficiency was verified by western blot. qRT-PCR was performed to measure the mRNA expression of p53. Western blot was used to evaluate the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oridonin; P53; Gastric cancer; Cell apoptosis; Mdm2.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200608

Serum and cerebrospinal fluid S100B concentrations in patients with neurocysticercosis
Provedor de dados: 56
Autores: Lima,J.E.; Walz,R.; Tort,A.; Souza,D.; Portela,L.; Bianchin,M.M.; Takayanagui,O.M.; Leite,J.P.
The clinical manifestations of neurocysticercosis (NC) are varied and depend on the number and location of cysts, as well as on the host immune response. Symptoms usually occur in NC when cysticerci enter a degenerative course associated with an inflammatory response. The expression of brain damage markers may be expected to increase during this phase. S100B is a calcium-binding protein produced and released predominantly by astrocytes that has been used as a marker of reactive gliosis and astrocytic death in many pathological conditions. The aim of the present study was to investigate the levels of S100B in patients in different phases of NC evolution. Cerebrospinal fluid and serum S100B concentrations were measured in 25 patients with NC: 14 patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurocysticercosis; S100B; Cerebrospinal fluid; Umin quotient; Glial marker.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100015

A novel noninvasive method to detect rejection after heart transplantation
Provedor de dados: 56
Autores: Hu,Jun; Xie,Xin; Li,Yuan; Wang,Shuang; Feng,Qing; Wang,Xin; Liang,Daoyan.
Prompt and accurate detection of rejection prior to pathological changes after organ transplantation is vital for monitoring rejections. Although biopsy remains the current gold standard for rejection diagnosis, it is an invasive method and cannot be repeated daily. Thus, noninvasive monitoring methods are needed. In this study, by introducing an IL-2 neutralizing monoclonal antibody (IL-2 N-mAb) and immunosuppressants into the culture with the presence of specific stimulators and activated lymphocytes, an activated lymphocyte-specific assay (ALSA) system was established to detect the specific activated lymphocytes. This assay demonstrated that the suppression in the ALSA test was closely related to the existence of specific activated lymphocytes. The ALSA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart transplantation rejection; Activated lymphocytes; Noninvasive method; Primed lymphocyte typing; Interleukin-2 neutralization monoclonal antibody; ALSA test.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200012

Clearance-inducing antibodies are responsible for protection against the acute phase of Trypanosoma cruzi infection in mice
Provedor de dados: 56
Autores: Umekita,L.F.; Ramos,D.P.; Mota,I.
A study was conducted on mice infected with strains Y and CL of Trypanosoma cruzi. The ability of anti-Y and anti-CL sera to induce complement-mediated lysis, immune clearance and protection against the acute phase of the infection was studied using homologous anti-Y or anti-CL serum tested with the Y or CL strain, or heterologous anti-Y serum tested with the CL strain or anti-CL serum tested with the Y strain. Complement-mediated lysis was induced by both homologous and heterologous antisera but protection was afforded only by homologous antisera. Immune clearance was induced by homologous but not by heterologous antisera. Antisera with high clearance ability were able to confer protection whereas antisera with high lytic ability were not. These results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Clearance; Complement-mediated lysis; Protection.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000009

Histopathological characteristics of a novel knock-in mouse prostate cancer model
Provedor de dados: 56
Autores: Wu,G.; Wang,D.; Wang,H.; Yuan,J.; Xuan,J.W.
Prostate cancer is relatively unique to man. There is no naturally occurring prostate cancer in the mouse. Pre-clinical studies involve the establishment of a genetically engineered mouse prostate cancer model with features close to those of the human situation. A new knock-in mouse adenocarcinoma prostate (KIMAP) model was established, which showed close-to-human kinetics of tumor development. In order to determine if the similar kinetics is associated with heterogeneous tumor architecture similar to the human situation, we utilized a new mouse histological grading system (Gleason analogous grading system) similar to the Gleason human grading system and flow cytometry DNA analysis to measure and compare the adenocarcinoma of the KIMAP model with human...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer model; KIMAP mouse model; Gleason grading system; Prostate adenocarcinoma.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600008

Acute effects of resistance exercise and intermittent intense aerobic exercise on blood cell count and oxidative stress in trained middle-aged women
Provedor de dados: 56
Autores: Cardoso,A.M.; Bagatini,M.D.; Roth,M.A.; Martins,C.C.; Rezer,J.F.P.; Mello,F.F.; Lopes,L.F.D.; Morsch,V.M.; Schetinger,M.R.C.
The aim of this study was to compare the effect of an intermittent intense aerobic exercise session and a resistance exercise session on blood cell counts and oxidative stress parameters in middle-aged women. Thirty-four women were selected and divided into three groups: RE group (performing 60 min of resistance exercises, N = 12), spinning group (performing 60 min of spinning, N = 12), and control group (not exercising regularly, N = 10). In both exercise groups, lymphocytes and monocytes decreased after 1-h recuperation (post-exercise) compared to immediately after exercise (P &lt; 0.05). Immediately after exercise, in both exercised groups, a significant increase in TBARS (from 16.5 ± 2 to 25 ± 2 for the spinning group and from 18.6 ± 1 to 28.2 ± 3 nmol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intermittent exercise; Anaerobic exercise; Oxidative stress; Immune suppression; Middle-aged women.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200010

Structural heart disease as the cause of syncope
Provedor de dados: 56
Autores: Guimarães,R.B.; Essebag,V.; Furlanetto,M.; Yanez,J.P.G.; Farina,M.G.; Garcia,D.; Almeida,E.D.; Stephan,L.; Lima,G.G.; Leiria,T.L.L.
We described the clinical evolution of patients with structural heart disease presenting at the emergency room with syncope. Patients were stratified according to their syncope etiology and available scores for syncope prognostication. Cox proportional hazard models were used to investigate the relationship between etiology of the syncope and event-free survival. Of the 82,678 emergency visits during the study period, 160 (0.16%) patients were there due to syncope, having a previous diagnosis of structural heart disease. During the median follow-up of 33.8±13.8 months, mean age at the qualifying syncope event was 68.3 years and 40.6% of patients were male. Syncope was vasovagal in 32%, cardiogenic in 57%, orthostatic hypotension in 6%, and of unknown...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Syncope; Hospitalization; Structural heart; Emergency.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400616

Pulmonary hypertension due to acute respiratory distress syndrome
Provedor de dados: 56
Autores: Ñamendys-Silva,S.A.; Santos-Martínez,L.E.; Pulido,T.; Rivero-Sigarroa,E.; Baltazar-Torres,J.A.; Domínguez-Cherit,G.; Sandoval,J.
Our aims were to describe the prevalence of pulmonary hypertension in patients with acute respiratory distress syndrome (ARDS), to characterize their hemodynamic cardiopulmonary profiles, and to correlate these parameters with outcome. All consecutive patients over 16 years of age who were in the intensive care unit with a diagnosis of ARDS and an in situ pulmonary artery catheter for hemodynamic monitoring were studied. Pulmonary hypertension was diagnosed when the mean pulmonary artery pressure was &gt;25 mmHg at rest with a pulmonary artery occlusion pressure or left atrial pressure &lt;15 mmHg. During the study period, 30 of 402 critically ill patients (7.46%) who were admitted to the ICU fulfilled the criteria for ARDS. Of the 30 patients with ARDS,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulmonary hypertension; Acute respiratory distress syndrome; Pulmonary artery catheter; Intensive care unit.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000904

Abundant immunohistochemical expression of dopamine D2 receptor and p53 protein in meningiomas: follow-up, relation to gender, age, tumor grade, and recurrence
Provedor de dados: 56
Autores: Trott,G.; Pereira-Lima,J.F.S.; Leães,C.G.S.; Ferreira,N.P.; Barbosa-Coutinho,L.M.; Oliveira,M.C.
Meningiomas are common, usually benign tumors, with a high postoperative recurrence rate. However, the genesis and development of these tumors remain controversial. We aimed to investigate the presence and implications of a mutated p53 protein and dopamine D2 receptor in a representative series of meningiomas and to correlate these findings with age, gender, tumor grade, and recurrence. Tumor tissue samples of 157 patients diagnosed with meningioma (37 males and 120 females, mean age 53.6±14.3 years) who underwent surgical resection between 2003 and 2012 at our institution were immunohistochemically evaluated for the presence of p53 protein and dopamine D2 receptor and were followed-up to analyze tumor recurrence or regrowth. Tumors were classified as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Receptors; Dopamine D2; Immunohistochemistry; Meningioma; Tumor suppressor protein p53; Recurrence.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500415

Contribution of CD4+ T cells to the early mechanisms of ischemia- reperfusion injury in a mouse model of acute renal failure
Provedor de dados: 56
Autores: Pinheiro,H.S.; Camara,N.O.S.; Noronha,I.L.; Maugeri,I.L.; Franco,M.F.; Medina,J.O.A.P.; Pacheco-Silva,A.
Renal ischemia-reperfusion (IR) injury is the major cause of acute renal failure in native and transplanted kidneys. Mononuclear leukocytes have been reported in renal tissue as part of the innate and adaptive responses triggered by IR. We investigated the participation of CD4+ T lymphocytes in the pathogenesis of renal IR injury. Male mice (C57BL/6, 8 to 12 weeks old) were submitted to 45 min of ischemia by renal pedicle clamping followed by reperfusion. We evaluated the role of CD4+ T cells using a monoclonal depleting antibody against CD4 (GK1.5, 50 µ, ip), and class II-major histocompatibility complex molecule knockout mice. Both CD4-depleted groups showed a marked improvement in renal function compared to the ischemic group, despite the fact that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ischemia-reperfusion; Kidney inflammation; Leukocytes; CD4+ T lymphocytes; Kidney transplantation; Acute renal failure.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400015

Hyperventilation in panic disorder patients and healthy first-degree relatives
Provedor de dados: 56
Autores: Nardi,A.E.; Valença,A.M.; Nascimento,I.; Mezzasalma,M.A.; Lopes,F.L.; Zin,W.A.
Our aim was to observe the induction of panic attacks by a hyperventilation challenge test in panic disorder patients (DSM-IV) and their healthy first-degree relatives. We randomly selected 25 panic disorder patients, 31 healthy first-degree relatives of probands with panic disorder and 26 normal volunteers with no family history of panic disorder. All patients had no psychotropic drugs for at least one week. They were induced to hyperventilate (30 breaths/min) for 4 min and anxiety scales were applied before and after the test. A total of 44.0% (N = 11) panic disorder patients, 16.1% (N = 5) of first-degree relatives and 11.5% (N = 3) of control subjects had a panic attack after hyperventilating (chi² = 8.93, d.f. = 2, P = 0.011). In this challenge test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Panic attacks; Respiration; Anxiety disorder; Ventilation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100009

Ethidium bromide-induced demyelination of the sciatic nerve of adult Wistar rats
Provedor de dados: 56
Autores: Riet-Correa,G.; Fernandes,C.G.; Pereira,L.A.V.; Graça,D.L.
Peripheral nerve ultrastructure was assessed after single or multiple local injections of the intercalating dye ethidium bromide. Thirty-four adult Wistar rats of both sexes were divided into five groups and maintained in a controlled environment with rat chow and water ad libitum throughout the experiment. The experimental animals were injected with 1 µl of 0.1% ethidium bromide in 0.9% saline into the central third of the left sciatic nerve 1 (group 1), 2 (group 2), 4 (group 3), 6 (group 4) or 8 (group 5) times. In groups 2 to 5 the injections were made at 28-day intervals. Control animals received the same amount of 0.9% saline. The animals were killed at different times after injection: group 1 at 7 days (2 rats) and 15 days (2 rats); for groups 2, 3,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Demyelination; Remyelination; Sciatic nerve; Ethidium bromide.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100014

Protein folding: a perspective for biology, medicine and biotechnology
Provedor de dados: 56
Autores: Yon,J.M.
At the present time, protein folding is an extremely active field of research including aspects of biology, chemistry, biochemistry, computer science and physics. The fundamental principles have practical applications in the exploitation of the advances in genome research, in the understanding of different pathologies and in the design of novel proteins with special functions. Although the detailed mechanisms of folding are not completely known, significant advances have been made in the understanding of this complex process through both experimental and theoretical approaches. In this review, the evolution of concepts from Anfinsen's postulate to the "new view" emphasizing the concept of the energy landscape of folding is presented. The main rules of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein folding; Energy landscape; Misfolding and disease.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400001

Relationship between matrix metalloproteinases and the occurrence and development of ovarian cancer
Provedor de dados: 56
Autores: Zhang,Y.; Chen,Q.
Ovarian cancer is one of the most malignant genital cancers, with a high mortality rate. Many researchers have suggested that matrix metalloproteinases (MMPs) have remarkably high expression in ovarian cancer tissues. MMPs are considered to be related to the occurrence, development, invasion and metastasis of ovarian cancer. Moreover, some studies have discovered that the unbalance between MMPs and tissue inhibitor of metalloproteinases (TIMPs) are associated with the malignant phenotype of tumors. This review summarizes the latest research progress of MMPs in ovarian cancer. The investigation of MMP mechanism in ovarian cancer will facilitate the development of effective anti-tumor drugs, and thereby improve the survival rate of patients with ovarian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovarian cancer; Matrix metalloproteinases (MMPs); Tissue inhibitor of metalloproteinases (TIMPs); Invasion; Metastasis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600301

Profile of thyroid hormones in breast cancer patients
Provedor de dados: 56
Autores: Saraiva,P.P.; Figueiredo,N.B.; Padovani,C.R.; Brentani,M.M.; Nogueira,C.R.
Estrogen involvement in breast cancer has been established; however, the association between breast cancer and thyroid diseases is controversial. Estrogen-like effects of thyroid hormone on breast cancer cell growth in culture have been reported. The objective of the present study was to determine the profile of thyroid hormones in breast cancer patients. Serum aliquots from 26 patients with breast cancer ranging in age from 30 to 85 years and age-matched normal controls (N = 22) were analyzed for free triiodothyronine (T3F), free thyroxine (T4F), thyroid-stimulating hormone (TSH), antiperoxidase antibody (TPO), and estradiol (E2). Estrogen receptor ß (ERß) was determined in tumor tissues by immunohistochemistry. Thyroid disease incidence was higher in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid hormone; Estrogen receptor; Breast cancer; Hyperthyroidism.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500014

YAP activation promotes the transdifferentiation of cardiac fibroblasts to myofibroblasts in matrix remodeling of dilated cardiomyopathy
Provedor de dados: 56
Autores: Jin,Bo; Zhu,Jun; Shi,Hai-Ming; Wen,Zhi-Chao; Wu,Bang-Wei.
Yes-associated protein (YAP) is an important regulator of cellular proliferation and transdifferentiation. However, little is known about the mechanisms underlying myofibroblast transdifferentiation in dilated cardiomyopathy (DCM). We investigated the role of YAP in the pathological process of cardiac matrix remodeling. A classic model of DCM was established in BALB/c mice by immunization with porcine cardiac myosin. Cardiac fibroblasts were isolated from neonatal Sprague-Dawley rats by density gradient centrifugation. The expression levels of α-smooth muscle actin (α-SMA) and collagen volume fraction (CVF) were significantly increased in DCM mice. Angiotensin II (Ang II)-mediated YAP activation promoted the proliferation and transdifferentiation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: YAP; Fibroblast; Cardiac remodeling; Dilated cardiomyopathy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100605

Clinical features of panic patients sensitive to hyperventilation or breath-holding methods for inducing panic attacks
Provedor de dados: 56
Autores: Nardi,A.E.; Valença,A.M.; Lopes,F.L.; Nascimento,I.; Mezzasalma,M.A.; Zin,W.A.
Our aim was to compare the clinical features of panic disorder (PD) patients sensitive to hyperventilation or breath-holding methods of inducing panic attacks. Eighty-five PD patients were submitted to both a hyperventilation challenge test and a breath-holding test. They were asked to hyperventilate (30 breaths/min) for 4 min and a week later to hold their breath for as long as possible, four times with a 2-min interval. Anxiety scales were applied before and after the tests. We selected the patients who responded with a panic attack to just one of the tests, i.e., those who had a panic attack after hyperventilating (HPA, N = 24, 16 females, 8 males, mean age ± SD = 38.5 ± 12.7 years) and those who had a panic attack after breath holding (BHPA, N = 20, 11...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anxiety disorder; Challenge tests; Panic disorder; Hyperventilation; Breath holding.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200013

Antiproliferative effect of urolithin A, the ellagic acid-derived colonic metabolite, on hepatocellular carcinoma HepG2.2.15 cells by targeting Lin28a/let-7a axis
Provedor de dados: 56
Autores: Qiu,Zhenpeng; Zhou,Junxuan; Zhang,Cong; Cheng,Ye; Hu,Junjie; Zheng,Guohua.
An abnormality in the Lin28/let-7a axis is relevant to the progression of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC), which could be a novel therapeutic target for this malignant tumor. The present study aimed to investigate the antiproliferative and anti-invasive effects of urolithin A in a stable full-length HBV gene integrated cell line HepG2.2.15 using CCK-8 and transwell assays. The RNA and protein expressions of targets were assessed by quantitative PCR and western blot, respectively. Results revealed that urolithin A induced cytotoxicity in HepG2.2.15 cells, which was accompanied by the cleavage of caspase-3 protein and down-regulation of Bcl-2/Bax ratio. Moreover, urolithin A suppressed the protein expressions of Sp-1, Lin28a,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urolithin A; Hepatocellular carcinoma; Lin28a; Let-7a; Cell proliferation; HBx.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700602

Aerobic training modulates T cell activation in elderly women with knee osteoarthritis
Provedor de dados: 56
Autores: Gomes,W.F.; Lacerda,A.C.R.; Brito-Melo,G.E.A.; Fonseca,S.F.; Rocha-Vieira,E.; Leopoldino,A.A.O.; Amorim,M.R.; Mendonça,V.A.
Osteoarthritis of the knee (kOA) is a disease that mainly affects the elderly and can lead to major physical and functional limitations. However, the specific effects of walking, particularly on the immune system, are unknown. Therefore, this study aimed to analyze the effect of 12 weeks of walking (3×/week) on the leukocyte profile and quality of life (QL) of elderly women with kOA. Sixteen women (age: 67±4 years, body mass index: 28.07±4.16 kg/m2) participated in a walking program. The variables were assessed before and after 12 weeks of training with a progressively longer duration (30–55 min) and higher intensity (72–82% of HRmax determined using a graded incremental treadmill test). The QL was assessed using the Medical Outcomes Study 36-Item Short...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoarthritis; Elderly; Exercise; CD4-positive T-lymphocytes; CD8-positive T-lymphocytes.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100705

Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine
Provedor de dados: 56
Autores: Guney,Y.; Hicsonmez,A.; Uluoglu,C.; Guney,H.Z.; Ozel Turkcu,U.; Take,G.; Yucel,B.; Caglar,G.; Bilgihan,A.; Erdogan,D.; Nalca Andrieu,M.; Kurtman,C.; Zengil,H.
We investigated the day-night differences in intestinal oxidative-injury and the inflammatory response following total body (TB) or abdominopelvic (AP) irradiation, and the influence of melatonin administration on tissue injury induced by radiation. Rats (male Wistar, weighing 220-280 g) in the irradiated groups were exposed to a dose of 8 Gy to the TB or AP region in the morning (resting period - 1 h after light onset) or evening (activity span - 13 h after light onset). Vehicle or melatonin was administered immediately before, immediately after and 24 h after irradiation (10, 2.0 and 10 mg/kg, ip, respectively) to the irradiated rats. AP (P &lt; 0.05) and TB (P &lt; 0.05) irradiation applied in the morning caused a significant increase in thiobarbituric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Irradiation; Melatonin; Circadian rhythm; Oxidative stress; Thiobarbituric acid reactive substances; Myeloperoxidase.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000002

The hemolytic component of cancer anemia: effects of osmotic and metabolic stress on the erythrocytes of rats bearing multifocal inoculations of the Walker 256 tumor
Provedor de dados: 56
Autores: Vido,A.A.; Cavalcanti,T.C.; Guimarães,F.; Vieira-Matos,A.N.; Rettori,O.
Cancer anemia is classified as an anemia of chronic diseases, although it is sometimes the first symptom of cancer. Cancer anemia includes a hemolytic component, important in the terminal stage when even transfused cells are rapidly destroyed. The presence of a chronic component and the terminal complications of the illness limit studies of the hemolytic component. A multifocal model of tumor growth was used here to simulate the terminal metastatic dissemination stage (several simultaneous inoculations of Walker 256 cells). The hemolytic component of anemia began 3-4 days after inoculation in 100% of the rats and progressed rapidly thereafter: Hb levels dropped from 14.9 ± 0.02 to 8.7 ± 0.06 from days 7 to 11 (~5 times the physiologically normal rate in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemolytic component; Cancer anemia; Osmotic fragility; Systemic effects; Walker 256 tumor.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700012

An empirical comparison of atypical bulimia nervosa and binge eating disorder
Provedor de dados: 56
Autores: Fontenelle,L.F.; Mendlowicz,M.V.; Moreira,R.O.; Appolinario,J.C.
The International Classification of Diseases, 10th edition (ICD-10) defines atypical bulimia nervosa (ABN) as an eating disorder that encompasses several different syndromes, including the DSM-IV binge eating disorder (BED). We investigated whether patients with BED can be differentiated clinically from patients with ABN who do not meet criteria for BED. Fifty-three obese patients were examined using the Structured Clinical Interview for DSM-IV and the ICD-10 criteria for eating disorders. All volunteers completed the Binge Eating Scale (BES), the Beck Depression Inventory, and the Symptom Checklist-90 (SCL-90). Individuals fulfilling criteria for both ABN and BED (N = 18), ABN without BED (N = 16), and obese controls (N = 19) were compared and contrasted....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Atypical bulimia nervosa; Binge eating disorder; Anxiety; Obesity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100014

A high-fructose diet induces insulin resistance but not blood pressure changes in normotensive rats
Provedor de dados: 56
Autores: Bezerra,R.M.N.; Ueno,M.; Silva,M.S.; Tavares,D.Q.; Carvalho,C.R.O.; Saad,M.J.A.; Gontijo,J.A.R.
Rats fed a high-fructose diet represent an animal model for insulin resistance and hypertension. We recently showed that a high-fructose diet containing vegetable oil but a normal sodium/potassium ratio induced mild insulin resistance with decreased insulin receptor substrate-1 tyrosine phosphorylation in the liver and muscle of normal rats. In the present study, we examined the mean blood pressure, serum lipid levels and insulin sensitivity by estimating in vivo insulin activity using the 15-min intravenous insulin tolerance test (ITT, 0.5 ml of 6 µg insulin, iv) followed by calculation of the rate constant for plasma glucose disappearance (Kitt) in male Wistar-Hannover rats (110-130 g) randomly divided into four diet groups: control, 1:3 sodium/potassium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High-fructose diet; Insulin resistance; Blood pressure; Hypertriglyceridemia; Plasma cholesterol.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900008

PD-1/PD-L1 regulates Treg differentiation in pregnancy-induced hypertension
Provedor de dados: 56
Autores: Jiang,Lai; Tang,Chaoliang; Gong,Yanping; Liu,Yujie; Rao,Jie; Chen,Suyu; Qu,Wanjun; Wu,Dabao; Lei,Lei; Chen,Ling.
Pregnancy-induced hypertension (PIH) causes significant maternal and fetal morbidity and mortality. A decreased number of regulatory T (Treg) cells is associated with the pathogenesis of PIH. The programmed cell death-1 (PD-1)/PD-ligand 1 (PD-L1) pathway is critical to normal pregnancy (NP) by promoting Treg cell development. However, the relationship between PD-1/PD-L1 and Treg differentiation in PIH has not been fully elucidated. In this study, venous blood was obtained from 20 NP and 58 PIH patients. Peripheral blood mononuclear cells (PBMCs) were isolated from venous blood. The levels of Treg-related cytokines (TGF-β, IL-10, and IL-35) in serum and PBMCs were measured by ELISA. The percentage of Treg cells in PBMCs was assessed by flow cytometry. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PD-1/PD-L1; Treg; PIH; Foxp3; Differentiation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800605

Preclinical evaluation of the antidiabetic effect of Eugenia jambolana seed powder in streptozotocin-diabetic rats
Provedor de dados: 56
Autores: Sridhar,S.B.; Sheetal,U.D.; Pai,M.R.S.M.; Shastri,M.S.
The world is facing an explosive increase in the incidence of diabetes mellitus and cost-effective complementary therapies are needed. The effects of Eugenia jambolana, a household remedy for diabetes, were studied. Streptozotocin diabetic female albino Wistar rats weighing 150-200 g (N = 6) were fed E. jambolana seed powder (250, 500 or 1000 mg/kg) for 15 days. Diabetic rats fed 500 and 1000 mg/kg seed powder showed an increase in body weight on day 20 in relation to day 5 (6 ± 4.7, 9 ± 7.8 vs diabetic control -16 ± 7.1 g, P &lt; 0.001), a decrease in fasting blood glucose (75 ± 11.9, 123 ± 14.4 vs diabetic control -34 ± 12.1 mg/dl, P &lt; 0.001), a difference in post-treatment fasting and peak blood glucose (38 ± 11.9, 36 ± 14.2 vs diabetic control 78 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Eugenia jambolana seed; Antidiabetic effect; Preclinical data; Liver glycogen; Toxicity studies.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300018

Association between tumor necrosis factor alpha rs1800629 polymorphism and risk of osteoarthritis in a Chinese population
Provedor de dados: 56
Autores: Chen,Jie; Wu,Yu; Yu,Jiannong; Shen,Jinming.
Osteoarthritis (OA) is the most common degenerative disease affecting articular cartilage. Some studies indicate that tumor necrosis factor alpha (TNF-α) gene rs1800629 polymorphism was associated with OA risk among Caucasian populations. To examine the role of this candidate gene in Asian populations, we conducted a hospital-based case-control study involving 257 knee OA patients and 305 controls in a Chinese population. Genotyping was performed using a custom-by-design 48-Plex single nucleotide polymorphism (SNP) Scan™ kit. Our study indicated that the AA genotype of TNF-α rs1800629 polymorphism was associated with increased risk of OA. Subsequently, we conducted a meta-analysis and found that rs1800629 polymorphism increased the risk of OA in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TNF-α; Osteoarthritis; Polymorphism; Meta-analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800604

Enhancement of anti-OVA IgG2c production in vivo by enalapril
Provedor de dados: 56
Autores: Almeida,L.C.; Muraro,L.S.; Albuquerque,D.A.
Angiotensin-converting enzyme (ACE) inhibitors have non-hemodynamic, pleiotropic effects on the immune response. The effects of ACE inhibitors on the production of cytokines and T-cell functions are well established. However, little is known on the effects of these medicines on humoral response to foreign antigens. In this study, we investigated the effect of enalapril treatment on ovalbumin (OVA)-specific IgG1 and IgG2c production in mice determined by ELISA. Two groups of 8-week-old C57BL/6 females mice (3–4/group) were subcutaneously immunized with OVA (10 μg/animal) in presence of Alhydrogel (1 mg/mouse) and boosted at day 21. The mice were treated with enalapril (5 mg/kg daily, po) or were left without treatment for one month. The animals were bled...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ACE inhibitors; Enalapril; Humoral response; IgG2c antibodies.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800603

Effects of chronic ethanol treatment on monoamine levels in rat hippocampus and striatum
Provedor de dados: 56
Autores: Vasconcelos,S.M.M.; Cavalcante,R.A.; Aguiar,L.M.V.; Sousa,F.C.F.; Fonteles,M.M.F.; Viana,G.S.B.
We studied the effects of ethanol on concentrations of noradrenaline (NE), dopamine (DA) and serotonin (5-HT) and their metabolites in rat hippocampus and striatum. Ethanol (2 or 4 g/kg, po, from a 20% aqueous solution) was administered daily to male Wistar rats (4-13 per group) for 30 days and animals were sacrificed 30 min or 48 h after the last administration. Monoamines were measured by HPLC and considered significant at P &lt; 0.05. A 47% increase in 5-HT levels was observed in the hippocampus with 4 g/kg ethanol in the 30-min protocol. Ethanol (2 and 4 g/kg) decreased DA (2114.5 ± 126.4 and 1785.1 ± 234.2 ng/g wet tissue, respectively) and 3,4-dihydroxyphenylacetic acid (DOPAC, 1477.6 ± 132.1 and 1218.8 ± 271.7 ng/g wet tissue, respectively) levels,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hippocampus; Striatum; Ethanol; Noradrenaline; Dopamine; Serotonin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200009

Effect of estrogen receptor-alpha (ESR1) gene polymorphism on high density lipoprotein levels in response to hormone replacement therapy
Provedor de dados: 56
Autores: Nogueira-de-Souza,N.C.; Guerreiro da Silva,I.D.C.; de Carvalho,C.V.; Pulchinelli,A.; Haidar,M.A.; Baracat,E.C.; Massad-Costa,A.M.
Studies have shown that estrogen replacement therapy and estrogen plus progestin replacement therapy alter serum levels of total, LDL and HDL cholesterol levels. However, HDL cholesterol levels in women vary considerably in response to hormone replacement therapy (HRT). A significant portion of the variability of these levels has been attributed to genetic factors. Therefore, we investigated the influence of estrogen receptor-alpha (ESR1) gene polymorphisms on HDL levels in response to postmenopausal HRT. We performed a prospective cohort study on 54 postmenopausal women who had not used HRT before the study and had no significant general medical illness. HRT consisted of conjugated equine estrogen and medroxyprogesterone acetate continuously for 1 year....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hormone replacement therapy; High-density lipoprotein cholesterol levels; Estrogen receptor-alpha; Polymorphism.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200003

Gender differences in the activities of aspirin-esterases in rat tissues
Provedor de dados: 56
Autores: Benedito,M.A.C.
The activities of aspirin (acetylsalicylic acid)-esterases were measured in several tissues (liver, kidney, adrenal glands, brain and serum) from adult male and female Wistar rats. In males, both aspirin-esterase I (assayed at pH 5.5) and II (assayed at pH 7.4) activities were higher in liver homogenates when compared to females (aspirin-esterase I: males 48.9 ± 4.8 (N = 8) and females 29.3 ± 4.2 (N = 8) nmol of salicylic acid formed min-1 mg protein-1; aspirin-esterase II: males 41.4 ± 4.1 (N = 8) and females 26.1 ± 4.5 (N = 8) nmol of salicylic acid formed min-1 mg protein-1, P&amp;lt;0.001). In serum, enzyme activity was higher in females than in males (aspirin-esterase I: males 0.85 ± 0.06 (N = 6) and females 1.18 ± 0.11 (N = 6) nmol of salicylic acid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspirin-esterases; Sex differences; Rats; Liver; Serum.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900002

A link between sleep loss, glucose metabolism and adipokines
Provedor de dados: 56
Autores: Padilha,H.G.; Crispim,C.A.; Zimberg,I.Z.; De-Souza,D.A.; Waterhouse,J.; Tufik,S.; de-Mello,M.T.
The present review evaluates the role of sleep and its alteration in triggering problems of glucose metabolism and the possible involvement of adipokines in this process. A reduction in the amount of time spent sleeping has become an endemic condition in modern society, and a search of the current literature has found important associations between sleep loss and alterations of nutritional and metabolic contexts. Studies suggest that sleep loss is associated with problems in glucose metabolism and a higher risk for the development of insulin resistance and type 2 diabetes mellitus. The mechanism involved may be associated with the decreased efficacy of regulation of the hypothalamus-pituitary-adrenal axis by negative feedback mechanisms in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucose metabolism; Insulin resistance; Sleep loss; Adipokines; Type 2 diabetes mellitus; Inflammatory status.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000003

Differential patterns of myosin Va expression during the ontogenesis of the rat hippocampus
Provedor de dados: 56
Autores: Brinn,L.S.; Pereira Leite,J.; Larson,R.E.; Martins,A.R.
Myosin Va is an actin-based, processive molecular motor protein highly enriched in the nervous tissue of vertebrates. It has been associated with processes of cellular motility, which include organelle transport and neurite outgrowth. The in vivo expression of myosin Va protein in the developing nervous system of mammals has not yet been reported. We describe here the immunolocalization of myosin Va in the developing rat hippocampus. Coronal sections of the embryonic and postnatal rat hippocampus were probed with an affinity-purified, polyclonal anti-myosin Va antibody. Myosin Va was localized in the cytoplasm of granule cells in the dentate gyrus and of pyramidal cells in Ammon's horn formation. Myosin Va expression changed during development, being...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myosin Va; Growth and development; Central nervous system; Hippocampus; Rat; Immunohistochemistry.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900012

Proliferation and osteogenic activity of fibroblasts induced with fibronectin
Provedor de dados: 56
Autores: Zhang,W.-H.; Li,X.-L.; Guo,Y.; Zhang,Y.
The aim of this study was to determine the proliferation and osteogenic activity of fibroblasts induced with fibronectin and their possible dose-dependent relationship. The fibroblasts obtained by tissue explants adherent method were induced with fibronectin at different concentrations of 0, 10, 20, 40, 60, and 80 μg/mL for 14 days. The 3H-thymidine and 3H-proline incorporation test was used to evaluate the synthesis of DNA and collagen by fibroblasts, respectively. The mineralized nodules and osteocalcin secretion, as vital osteogenic indicators, were detected with tetracycline labeling and 125I-labeled competitive immunoassay, respectively. Fibronectin significantly increased the synthesis of DNA and collagen by fibroblasts, especially at the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibroblast; Fibronectin; Proliferation; Osteogenesis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000604

The role of oxytocin in cardiovascular regulation
Provedor de dados: 56
Autores: Gutkowska,J.; Jankowski,M.; Antunes-Rodrigues,J.
Studies of body volume expansion have indicated that lesions of the anteroventral third ventricle and median eminence block the release of atrial natriuretic peptide (ANP) into the circulation. Detailed analysis of the lesions showed that activation of oxytocin (OT)-ergic neurons is responsible for ANP release, and it has become clear that activation of neuronal circuitry elicits OT secretion into the circulation, activating atrial OT receptors and ANP release from the heart. Subsequently, we have uncovered the entire functional OT system in the rat and the human heart. An abundance of OT has been observed in the early development of the fetal heart, and the capacity of OT to generate cardiomyocytes (CMs) has been demonstrated in various types of stem...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxytocin; Natriuretic peptides; Heart; Cardiomyogenesis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300206

Anti-cyclic citrullinated peptide antibodies and rheumatoid factor in leprosy patients with articular involvement
Provedor de dados: 56
Autores: Ribeiro,S.L.E.; Pereira,H.L.A.; Silva,N.P.; Neves,R.M.S.; Sato,E.I.
The objective of the present research was to evaluate the usefulness of anti-cyclic citrullinated peptide (anti-CCP) antibodies and the IgM rheumatoid factor (IgM RF) test for the differential diagnosis of leprosy with articular involvement and rheumatoid arthritis (RA). Anti-CCP antibodies and IgM RF were measured in the sera of 158 leprosy patients (76 with and 82 without articular involvement), 69 RA patients and 89 healthy controls. Leprosy diagnosis was performed according to Ridley and Jopling classification criteria and clinical and demographic characteristics of leprosy patients were collected by a standard questionnaire. Leprosy patients with any concomitant rheumatic disease were excluded. Serum samples were obtained from all participants and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leprosy; Rheumatoid arthritis; Anti-cyclic citrullinated peptide antibodies; Rheumatoid factor.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100010

Characterization of ß-trypsin at acid pH by differential scanning calorimetry
Provedor de dados: 56
Autores: Bittar,E.R.; Caldeira,F.R.; Santos,A.M.C.; Günther,A.R.; Rogana,E.; Santoro,M.M.
Trypsin is a serino-protease with a polypeptide chain of 223 amino acid residues and contains six disulfide bridges. It is a globular protein with a predominance of antiparallel ß-sheet and helix in its secondary structure and has two domains with similar structures. We assessed the stability of ß-trypsin in the acid pH range using microcalorimetric (differential scanning calorimetry) techniques. Protein concentrations varied in the range of 0.05 to 2.30 mg/ml. Buffer solutions of 50.0 mM ß-alanine and 20.0 mM CaCl2 at different pH values (from 2.0 to 4.2) and concentrations of sorbitol (1.0 and 2.0 M), urea (0.5 M) or guanidinium hydrochloride (0.5 and 1.0 M) were used. The data suggest that we are studying the same conformational transition of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SS-Trypsin; Thermal denaturation; Differential scanning calorimetry.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200003

Astragalus polysaccharides improve cardiomyopathy in STZ-induced diabetic mice and heterozygous (SOD2+/-) knockout mice
Provedor de dados: 56
Autores: Ju,J.; Chen,W.; Lai,Y.; Wang,L.; Wang,H.; Chen,W.J.; Zhao,X.; Ye,H.; LI,Y.; Zhang,Y.
Oxidative stress plays an important role in the development of diabetic cardiomyopathy. In the present study, we determined whether the effect of astragalus polysaccharides (APS) on diabetic cardiomyopathy was associated with its impact on oxidative stress. Streptozotocin (STZ)-induced diabetic mice and heterozygous superoxide dismutase (SOD2+/-) knockout mice were administered APS. The hemodynamics, cardiac ultrastructure, and the apoptosis, necrosis and proliferation of cardiomyocytes were assessed to evaluate the effect of APS on diabetic and oxidative cardiomyopathy. Furthermore, H2O2 formation, oxidative stress/damage, and SOD activity in cardiomyocytes were evaluated to determine the effects of APS on cardiac oxidative stress. APS therapy improved...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Cardiomyopathy; Oxidative stress; Superoxide dismutase.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800601

Capecitabine maintenance therapy in patients with recurrent or metastatic breast cancer
Provedor de dados: 56
Autores: Si,W.; Zhu,Y.Y.; Li,Y.; Gao,P.; Han,C.; You,J.H.; Linghu,R.X.; Jiao,S.C.; Yang,J.L.
Our objective was to investigate the efficacy and safety of capecitabine maintenance therapy (CMT) after capecitabine-based combination chemotherapy in patients with metastatic breast cancer. The clinical data of 139 metastatic breast cancer patients treated from March 2008 to May 2012 with capecitabine-based combination chemotherapy were retrospectively analyzed. When initial disease control was achieved by the combination chemotherapy, we used CMT for 50 patients, while 37 patients were treated with a different (non-CMT) maintenance therapy. We compared time to progression (TTP), objective response rate, disease control rate, clinical benefit rate, and safety of the two groups, and a sub-group analysis was performed according to pathological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capecitabine; Maintenance therapy; Metastatic breast cancer.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201074

Culture of bovine ovarian follicle wall sections maintained the highly estrogenic profile under basal and chemically defined conditions
Provedor de dados: 56
Autores: Vasconcelos,R.B.; Salles,L.P.; Oliveira e Silva,I.; Gulart,L.V.M.; Souza,D.K.; Torres,F.A.G.; Bocca,A.L.; Rosa e Silva,A.A.M.
Follicle cultures reproduce in vitro the functional features observed in vivo. In a search for an ideal model, we cultured bovine antral follicle wall sections (FWS) in a serum-free defined medium (DM) known to induce 17β-estradiol (E2) production, and in a nondefined medium (NDM) containing serum. Follicles were sectioned and cultured in NDM or DM for 24 or 48 h. Morphological features were determined by light microscopy. Gene expression of steroidogenic enzymes and follicle-stimulating hormone (FSH) receptor were determined by RT-PCR; progesterone (P4) and E2 concentrations in the media were measured by radioimmunoassay. DM, but not NDM, maintained an FWS morphology in vitro that was similar to fresh tissue. DM also induced an increase in the expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovary follicle wall section; FSH receptor; Gene expression; Steroidogenic enzymes; Hormone steroids; Morphological aspect.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800700

Arterial blood pressure of term newborns during the first week of life
Provedor de dados: 56
Autores: Nascimento,M.C.V.A.; Xavier,C.C.; Goulart,E.M.A.
The progressive behavior of the blood pressure of term newborns during the first week of life was assessed by the simultaneous use of oscillometric and Doppler methods. A total of 174 term neonates born at the Municipal Hospital Odilon Behrens in Belo Horizonte, from March 1996 to February 1997, were prospectively assessed. The oscillometric and Doppler ultrasonic methods were simultaneously used for four consecutive recordings obtained at 12 ± 6, 24 ± 6 and 72 ± 24 h and on the 7th ± 1 day of life. The combined use of the two methods simplified the procedure, with automatic cuff inflation and deflation, and speed was properly controlled with an automatic pressure monitor. The procedure was performed using a Y-connection to the mercury sphygmomanometer,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood pressure; Doppler method; Oscillometric method; Term newborn.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800007

Hippocampal overexpression of Down syndrome cell adhesion molecule in amyloid precursor protein transgenic mice
Provedor de dados: 56
Autores: Jia,Y.L.; Fu,Z.X.; Zhang,B.H.; Jia,Y.J.
Down syndrome cell adhesion molecule (DSCAM) is located within the Down syndrome critical region of chromosome 21. DSCAM is a broadly expressed neurodevelopmental protein involved in synaptogenesis, neurite outgrowth, and axon guidance. We previously demonstrated DSCAM overexpression in the cortex of amyloid precursor protein (APP) transgenic mice, suggesting possible regulatory interactions between APP and DSCAM. APP mice exhibit deficits in hippocampus-dependent learning and memory. In this preliminary study, we examined age-related changes in DSCAM expression within the hippocampus in 16 APP transgenic mice (1, 3, 6 and 12 months old). Hippocampus-dependent spatial memory was assessed in APP mice and age-matched wild type littermates (WTs) using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Down syndrome cell adhesion molecule; Amyloid precursor protein; Transgenic mouse; Hippocampus; Dementia.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600603

Ferulic acid lowers body weight and visceral fat accumulation via modulation of enzymatic, hormonal and inflammatory changes in a mouse model of high-fat diet-induced obesity
Provedor de dados: 56
Autores: de Melo,T.S.; Lima,P.R.; Carvalho,K.M.M.B.; Fontenele,T.M.; Solon,F.R.N.; Tomé,A.R.; de Lemos,T.L.G.; da Cruz Fonseca,S.G.; Santos,F.A.; Rao,V.S.; de Queiroz,M.G.R.
Previous studies have reported on the glucose and lipid-lowering effects of ferulic acid (FA) but its anti-obesity potential has not yet been firmly established. This study investigated the possible anti-obesitogenic effects of FA in mice fed a high-fat diet (HFD) for 15 weeks. To assess the antiobesity potential of FA, 32 male Swiss mice, weighing 20–25 g (n=6–8 per group) were fed a normal diet (ND) or HFD, treated orally or not with either FA (10 mg/kg) or sibutramine (10 mg/kg) for 15 weeks and at the end of this period, the body weights of animals, visceral fat accumulation, plasma levels of glucose and insulin hormone, amylase and lipase activities, the satiety hormones ghrelin and leptin, and tumor necrosis factor-α (TNF-α) and monocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ferulic acid; Anti-obesity; High-fat diet; Leptin; Lipase; Tumor necrosis factor-α.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100603

A mutant cell line partially responsive to both IFN-a and IFN-g
Provedor de dados: 56
A recessive mutant cell line, B7, which is partially responsive to both interferon (IFN)-&lt;FONT FACE="Symbol"&gt; a&lt;/font&gt; and IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; is described. B7 was FACS sorted from a cellular pool, which was obtained from the parental cell line 2C4, after several rounds of mutagenesis. The partial responsiveness to IFN was observed both in terms of expression of cell surface markers (CD2, class I and II HLAs) and mRNA expression of IFN-stimulated genes (9-27; 6-16; 2'-5' OAS; GBP and HLA-DRa). A genetic cross with the U4 mutant (JAK1-, a member of the Janus family of nonreceptor tyrosine kinase) did not restore full IFN-responsiveness to B7, and JAK1 cDNA transfection into B7 restored the wild phenotype of the cell...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Interferons; Transcriptional activation; Molecular genetics; JAK1; FACS analysis.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100007

Low density lipoprotein modified silica nanoparticles loaded with docetaxel and thalidomide for effective chemotherapy of liver cancer
Provedor de dados: 56
Autores: Ao,Man; Xiao,Xu; Ao,Yazhou.
In the present study, we successfully developed a docetaxel (DTX) and thalidomide (TDD) co-delivery system based on low density lipoprotein (LDL) modified silica nanoparticles (LDL/SLN/DTX/TDD). By employing the tumor homing property of LDL and the drug-loading capability of silica nanoparticles, the prepared LDL/SLN/DTX/TDD was expected to locate and specifically deliver the loaded drugs (DTX and TDD) to achieve effective chemotherapy of liver cancer. In vitro analysis revealed that nano-sized LDL/SLN/DTX/TDD with decent drug loading capabilities was able to increase the delivery efficiency by targeting the low density lipoprotein receptors, which were overexpressed on HepG2 human hepatocellular liver carcinoma cell line, which exerted better cytotoxicity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Docetaxel; Thalidomide; Low density lipoprotein; Silica nanoparticles; Liver cancer.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300613

Nitric oxide synthase blockade and body fluid volumes
Provedor de dados: 56
Autores: Balaszczuk,A.M.; Tomat,A.; Bellucci,S.; Fellet,A.; Arranz,C.
The influence of chronic nitric oxide synthase inhibition with N G-nitro-L-arginine methyl ester (L-NAME) on body fluid distribution was studied in male Wistar rats weighing 260-340 g. Extracellular, interstitial and intracellular spaces, as well as plasma volume were measured after a three-week treatment with L-NAME (~70 mg/kg per 24 h in drinking water). An increase in extracellular space (16.1 ± 1.1 vs 13.7 ± 0.6 ml/100 g in control group, N = 12, P&lt;0.01), interstitial space (14.0 ± 0.9 vs 9.7 ± 0.6 ml/100 g in control group, P&lt;0.001) and total water (68.7 ± 3.9 vs 59.0 ± 2.9 ml/100 g, P&lt;0.001) was observed in the L-NAME group (N = 8). Plasma volume was lower in L-NAME-treated rats (2.8 ± 0.2 ml/100 g) than in the control group (3.6 ± 0.1...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Kidney; Heart; Body fluid; Hypertension; Extracellular space; Intracellular space; L-NAME.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100019

Detection of Mycoplasma pneumoniae and Chlamydia pneumoniae in ruptured atherosclerotic plaques
Provedor de dados: 56
Autores: Higuchi,M.L.; Sambiase,N.; Palomino,S.; Gutierrez,P.; Demarchi,L.M.; Aiello,V.D.; Ramires,J.A.F.
This paper reports what is apparently the first observation of Mycoplasma pneumoniae in association with Chlamydia pneumoniae in thrombosed ruptured atheromas. We performed electron microscopy and in situ hybridization in specimens from three patients who died of acute myocardial infarction. These patients had typical symptoms of acute ischemic syndrome. Mycoplasmas were present mainly in the lipid core of the ruptured thrombosed plaque. Vulnerable atheromas are rich in cholesterol and may favor the growth of mycoplasmas, the only microorganisms that require cholesterol for survival. We suggest that the association of Mycoplasma pneumoniae and Chlamydia pneumoniae may increase the virulence of these microorganisms, favoring proliferation, plaque...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mycoplasma pneumoniae; Chlamydia pneumoniae; Atherosclerosis; Vulnerable plaque; Inflammation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900005

Antioxidant responses of Laeonereis acuta (Polychaeta) after exposure to hydrogen peroxide
Provedor de dados: 56
Autores: Rosa,C.E. da; Bianchini,A.; Monserrat,J.M.
The effects of H2O2 were evaluated in the estuarine worm Laeonereis acuta (Polychaeta, Nereididae) collected at the Patos Lagoon estuary (Southern Brazil) and maintained in the laboratory under controlled salinity (10 psu diluted seawater) and temperature (20°C). The worms were exposed to H2O2 (10 and 50 µM) for 4, 7, and 10 days and the following variables were determined: oxygen consumption, catalase (CAT) and glutathione peroxidase activity in both the supernatant and pellet fractions of whole body homogenates. The concentrations of non-protein sulfhydryl and lipid peroxides (LPO) were also measured. The oxygen consumption response was biphasic, decreasing after 4 days and increasing after 7 and 10 days of exposure to 50 µM H2O2 (P &lt; 0.05). At the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Worms; Laeonereis acuta; Hydrogen peroxide; Oxidative stress; Antioxidant enzymes; Oxygen consumption.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200007

Distribution of calcium-binding proteins in the chick visual system
Provedor de dados: 56
Autores: Pfeiffer,C.P.; Britto,L.R.G.
The calcium-binding proteins calbindin (CB), calretinin (CR), and parvalbumin (PV) have been extensively studied over the last decade since they appear to be important as buffers of intracellular calcium. In the present study we investigated the distribution of these proteins in the chick visual system by means of conventional immunocytochemistry. The results indicated that CB, CR, and PV are widely distributed in retinorecipient areas of the chick brain. In some regions, all three calcium-binding proteins were present at different intensities and often in different neurons such as in the dorsolateral thalamic complex. In other areas, such as the nucleus geniculatus lateralis ventralis, only CB and CR were detected, whereas PV was absent. These results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calbindin; Calretinin; Chick; Immunohistochemistry; Parvalbumin; Visual system.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100009

Different biochemical strategies of two Neotropical fish to cope with the impairment of nitrogen excretion during air exposure
Provedor de dados: 56
Autores: Polez,V.L.P.; Moraes,G.; Santos Neto,C.
The exposure of fish to air is normally expected to interfere with the nitrogen excretion process. Hoplias malabaricus and Hoplerythrinus unitaeniatus, two teleost species, display distinct behaviors in response to decreases in natural reservoir water levels, although they may employ similar biochemical strategies. To investigate this point, plasma levels of ammonia, urea, uric acid, and the two urea cycle enzymes, ornithine carbamoyl transferase (OCT) and arginase (ARG), as well as glutamine synthetase (GS) were determined for both species after exposure to air. Plasma ammonia increased gradually during exposure to air, but only H. malabaricus showed increased concentrations of urea. Plasma uric acid remained very low in both fish. Enzymatic activities...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hoplias malabaricus; Hoplerythrinus unitaeniatus; Fish; Urea cycle enzymes; Biochemical adaptation; Air exposure.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200017

Variations in gastric compliance induced by acute blood volume changes in anesthetized rats
Provedor de dados: 56
Autores: Graça,J.R.V.; Leal,P.R.L.; Gondim,F.A.A.; Rola,F.H.; Santos,A.A.
The impact of acute volume imbalances on gastric volume (GV) was studied in anesthetized rats (250-300 g). After cervical and femoral vessel cannulation, a balloon catheter was positioned in the proximal stomach. The opposite end of the catheter was connected to a barostat with an electronic sensor coupled to a plethysmometer. A standard ionic solution was used to fill the balloon (about 3.0 ml) and the communicating vessel system, and to raise the reservoir liquid level 4 cm above the animals' xiphoid appendix. Due to constant barostat pressure, GV values were considered to represent the gastric compliance index. All animals were monitored for 90 min. After a basal interval, they were randomly assigned to normovolemic, hypervolemic, hypovolemic or...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Blood volume; Gastric compliance; Gastrointestinal motility; Hemorrhage; Hypervolemia.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300018

Immune strategies using single-component LipL32 and multi-component recombinant LipL32-41-OmpL1 vaccines against leptospira
Provedor de dados: 56
Autores: Feng,C.Y.; Li,Q.T.; Zhang,X.Y.; Dong,K.; Hu,B.Y.; Guo,X.K.
Genes encoding lipoproteins LipL32, LipL41 and the outer-membrane protein OmpL1 of leptospira were recombined and cloned into a pVAX1 plasmid. BALB/c mice were immunized with LipL32 and recombined LipL32-41-OmpL1 using DNA-DNA, DNA-protein and protein-protein strategies, respectively. Prime immunization was on day 1, boost immunizations were on day 11 and day 21. Sera were collected from each mouse on day 35 for antibody, cytokine detection and microscopic agglutination test while spleen cells were collected for splenocyte proliferation assay. All experimental groups (N = 10 mice per group) showed statistically significant increases in antigen-specific antibodies, in cytokines IL-4 and IL-10, as well as in the microscopic agglutination test and splenocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immune strategy; Recombinant vaccine; Leptospira; Lipoproteins LipL32; LipL41.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900005

Changes in NAD/ADP-ribose metabolism in rectal cancer
Provedor de dados: 56
Autores: Yalcintepe,L.; Turker-Sener,L.; Sener,A.; Yetkin,G.; Tiryaki,D.; Bermek,E.
The extent of ADP-ribosylation in rectal cancer was compared to that of the corresponding normal rectal tissue. Twenty rectal tissue fragments were collected during surgery from patients diagnosed as having rectal cancer on the basis of pathology results. The levels of ADP-ribosylation in rectum cancer tissue samples (95.9 ± 22.1 nmol/ml) was significantly higher than in normal tissues (11.4 ± 4 nmol/ml). The level of NAD+ glycohydrolase and ADP-ribosyl cyclase activities in rectal cancer and normal tissue samples were measured. Cancer tissues had significantly higher NAD+ glycohydrolase and ADP-ribosyl cyclase activities than the control tissues (43.3 ± 9.1 vs 29.2 ± 5.2 and 6.2 ± 1.6 vs 1.6 ± 0.4 nmol mg-1 min-1). Approximately 75% of the NAD+...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rectal cancer; ADP-ribosylation; NAD+; NAD+ glycohydrolase; Poly(ADP-ribose) polymerase.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300006

Expression of p53 protein in pituitary adenomas
Provedor de dados: 56
Autores: Oliveira,M.C.; Marroni,C.P.; Pizarro,C.B.; Pereira-Lima,J.F.; Barbosa-Coutinho,L.M.; Ferreira,N.P.
Inactivating mutations of TP53, a tumor suppressor gene, are associated with abnormal cell proliferation. Although p53 expression is common in many human malignancies, p53 protein has seldom been evaluated in pituitary tumors. When detected, the percentage of p53-positive cells is low, and, in general, it is exclusive for invasive lesions. The aim of the present study was to use immunohistochemistry to determine the presence of p53 protein in pituitary adenomas from tumor samples of 163 surgeries performed in 148 patients (40% male, 60% female). In 35% of the cases the adenoma was nonfunctional, while in the others it was associated with PRL, GH and/or ACTH endocrine hypersecretion syndrome. Macroadenomas were observed in 83.2% of the cases with available...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pituitary; Pituitary adenoma; Invasive adenoma; P53; Immunohistochemistry.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500008

Relationship between disease severity and quality of life in patients with chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Sanchez,F.F.; Faganello,M.M.; Tanni,S.E.; Lucheta,P.A.; Padovani,C.R.; Godoy,I.
Few studies have evaluated the relationship between Airways Questionnaire 20 (AQ20), a measure of the quality of life, scores and physiological outcomes or with systemic markers of disease in patients with chronic obstructive pulmonary disease (COPD). The aim of the present study was to investigate the relationship of forced expiratory volume in 1 s (FEV1), body mass index, fat-free mass index, 6-min walk test (6MWT) results, dyspnea sensation and peripheral oxygen saturation (SpO2) with the quality of life of COPD patients. Ninety-nine patients with COPD (mean age: 64.2 ± 9.2 years; mean FEV1: 60.4 ± 25.2% of predicted) were evaluated using spirometry, body composition measurement and the 6MWT. The baseline dyspnea index (BDI) and the Modified Medical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulmonary disease; Chronic obstructive pulmonary disease; St. George's Respiratory Questionnaire; Airways Questionnaire 20; Respiratory system.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000005

Conditioned medium from activated spleen cells supports the survival of rat retinal cells in vitro
Provedor de dados: 56
Autores: Sholl-Franco,A.; Araujo,E.G.
Cytokines are a heterogeneous group of molecules that have been associated with several functions in the nervous system, such as survival and differentiation of neuronal and glial cells. In the present study, we demonstrated that conditioned medium from spleen cells activated with concanavalin A increased neuritogenesis and survival of retinal cells, as measured by biochemical and morphological criteria. Our data showed that conditioned medium induced a five-fold increase in the amount of protein after 120 h in vitro. This effect was not inhibited by the blockade of voltage-dependent L-type calcium channels with 5.0 µM nifedipine. However, the use of an intracellular calcium chelator (15.0 µM BAPTA-AM) inhibited this effect. Our results support the idea...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Growth factor; Neuroimmunology; Retina; Development.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100006

An acid-sensing ion channel that detects ischemic pain
Provedor de dados: 56
Autores: Naves,L.A.; McCleskey,E.W.
Ischemic pain occurs when there is insufficient blood flow for the metabolic needs of an organ. The pain of a heart attack is the prototypical example. Multiple compounds released from ischemic muscle likely contribute to this pain by acting on sensory neurons that innervate muscle. One such compound is lactic acid. Here, we show that ASIC3 (acid-sensing ion channel #3) has the appropriate expression pattern and physical properties to be the detector of this lactic acid. In rats, it is expressed only in sensory neurons and then only on a minority (~40%) of these. Nevertheless, it is expressed at extremely high levels on virtually all dorsal root ganglion sensory neurons that innervate the heart. It is extraordinarily sensitive to protons (Hill slope 4,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pain; Ischemia; Dorsal root ganglia; Acid-sensing ion channels; Lactic acidosis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100001

Protective effect of bronchial challenge with hypertonic saline on nocturnal asthma
Provedor de dados: 56
Autores: Borges,M.C.; Ferraz,E.; Terra-Filho,J.; Vianna,E.O.
Inhalation of hypertonic saline (HS) causes bronchoconstriction in asthmatic subjects. Repeated inhalation of HS leads to substantially reduced bronchoconstriction, known as the refractory period. Refractoriness due to different stimuli has also been described (cross-refractoriness). Nocturnal asthma is defined as an increase in symptoms, need for medication, airway responsiveness, and/or worsening of lung function that usually occurs from 4 to 6 am. Our objective was to determine the effect of refractoriness on nocturnal asthma. The challenge test consisted of inhalations of 4.5% saline with increasing durations until a reduction of 20% in forced expiratory volume in 1 s (FEV1) (PD20HS) or total time of 15.5 min. Twelve subjects with nocturnal asthma were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Refractory period; Bronchial challenge; Hypertonic saline; Bronchial provocation test.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300006

Human papillomavirus infection and its association with cervical dysplasia in Ecuadorian women attending a private cancer screening clinic
Provedor de dados: 56
Autores: Brown,C.R.; Leon,M.L.; Muñoz,K.; Fagioni,A.; Amador,L.G.; Frain,B.; Tu,W.; Qadadri,B.; Brown,D.R.
Women living in Latin American countries bear a disproportionate burden of cervical cancer, a condition caused by infection with the human papillomavirus (HPV). We performed a study in Santa Elena, Guayas (currently Santa Elena Province), Ecuador, to determine how often HPV could be detected in women attending a private cancer screening clinic. Participants underwent a Pap test, and vaginal and cervical swabs were performed for HPV testing by the polymerase chain reaction (PCR). Each participant completed a verbally administered survey. The mean age of 302 participants was 37.7 years (range 18 to 78 years). The majority of cervical and vaginal specimens contained sufficient DNA to perform PCR. Overall, 24.2% of the participants had either a cervical or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Cervical dysplasia; Ecuador.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700007

Neonatal tobacco smoke reduces thermogenesis capacity in brown adipose tissue in adult rats
Provedor de dados: 56
Autores: Peixoto,T.C.; Moura,E.G.; Oliveira,E.; Younes-Rapozo,V.; Soares,P.N.; Rodrigues,V.S.T.; Santos,T.R.; Peixoto-Silva,N.; Carvalho,J.C.; Calvino,C.; Conceição,E.P.S.; Guarda,D.S.; Claudio-Neto,S.; Manhães,A.C.; Lisboa,P.C.
Maternal smoking is a risk factor for progeny obesity. We have previously shown, in a rat model of neonatal tobacco smoke exposure, a mild increase in food intake and a considerable increase in visceral adiposity in the adult offspring. Males also had secondary hyperthyroidism, while females had only higher T4. Since brown adipose tissue (BAT) hypofunction is related to obesity, here we tested the hypothesis that higher levels of thyroid hormones are not functional in BAT, suggesting a lower metabolic rate. We evaluated autonomic nerve activity in BAT and its function in adult rats that were exposed to tobacco smoke during lactation. At birth, litters were adjusted to 3 male and 3 female pups/litter. From postnatal day (PND) 3 to 21, Wistar lactating rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tobacco smoke Suckling period Developmental plasticity; Obesity Autonomic function.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600602

Effect of intracerebroventricularly injected insulin on urinary sodium excretion by cerebroventricular streptozotocin-treated rats
Provedor de dados: 56
Autores: Macedo,R.F.; Furlan,F.C.; Marshall,P.S.; Michelotto,J.B.; Gontijo,J.A.R.
Recent evidence suggests that insulin may influence many brain functions. It is known that intracerebroventricular (icv) injection of nondiabetogenic doses of streptozotocin (STZ) can damage insulin receptor signal transduction. In the present study, we examined the functional damage to the brain insulin receptors on central mechanisms regulating glomerular filtration rate and urinary sodium excretion, over four periods of 30 min, in response to 3 µl insulin or 0.15 NaCl (vehicle) injected icv in STZ-treated freely moving Wistar-Hannover rats (250-300 g). The icv cannula site was visually confirmed by 2% Evans blue infusion. Centrally administered insulin (42.0 ng/µl) increased the urinary output of sodium (from 855.6 ± 85.1 to 2055 ± 310.6 delta%/min; N =...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Central nervous system; Insulin; Natriuresis; Streptozotocin; Lithium clearance.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900008

Meningoencephalitis caused by a zygomycete fungus (Basidiobolus) associated with septic shock in an immunocompetent patient: 1-year follow-up after treatment
Provedor de dados: 56
Autores: Auxiliadora-Martins,M.; Alkmim-Teixeira,G.C.; Machado-Viana,J.; Nicolini,E.A.; Martins-Filho,O.A.; Bellissimo-Rodrigues,F.; Carlotti Jr.,C.G.; Basile-Filho,A.
Zygomycosis is an infection caused by opportunistic fungi of the Zygomycetes class, specifically those from the Mucorales and Entomophthorales orders. It is an uncommon disease, mainly restricted to immunocompromised patients. We report a case of a 73-year-old male patient with a history of fever (39°C) lasting for 1 day, accompanied by shivering, trembling, and intense asthenia. The patient was admitted to the intensive care unit with complex partial seizures, and submitted to orotracheal intubation and mechanical ventilation under sedation with midazolam. The electroencephalogram showed evidence of non-convulsive status epilepticus. There is no fast specific laboratory test that permits confirmation of invasive fungal disease. Unless the physician...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Zygomycete; Septic shock; Status epilepticus; Meningoencephalitis; ICU setting.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800013

IL-6 and TNF-α serum levels are associated with early death in community-acquired pneumonia patients
Provedor de dados: 56
Autores: Bacci,M.R.; Leme,R.C.P.; Zing,N.P.C.; Murad,N.; Adami,F.; Hinnig,P.F.; Feder,D.; Chagas,A.C.P.; Fonseca,F.L.A.
Community-acquired pneumonia (CAP) is amongst the leading causes of death worldwide. As inflammatory markers, cytokines can predict outcomes, if interpreted together with clinical data and scoring systems such as CURB-65, CRB, and Acute Physiology and Chronic Health Evaluation II (APACHE II). The aim of this study was to determine the impact of inflammatory biomarkers on the early mortality of hospitalized CAP patients. Twenty-seven CAP patients needing hospitalization were enrolled for the study and samples of interleukin-1 (IL-1) and interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), C-reactive protein (CRP), and homocystein were collected at the time of admission (day 1) as well as on the seventh day of the treatment. There was a significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Community acquired pneumonia; Interleukin-6; Tumor necrosis factor α; Sepsis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500427

McFarland nephelometer as a simple method to estimate the sensitivity of the polymerase chain reaction using Mycobacterium tuberculosis as a research tool
Provedor de dados: 56
Autores: Bollela,V.R.; Sato,D.N.; Fonseca,B.A.L.
Polymerase chain reaction (PCR) has been widely investigated for the diagnosis of tuberculosis. However, before this technique is applied on clinical samples, it needs to be well standardized. We describe the use of McFarland nephelometer, a very simple approach to determine microorganism concentration in solution, for PCR standardization and DNA quantitation, using Mycobacterium tuberculosis as a model. Tuberculosis is an extremely important disease for the public health system in developing countries and, with the advent of AIDS, it has also become an important public health problem in developed countries. Using Mycobacterium tuberculosis as a research model, we were able to detect 3 M. tuberculosis genomes using the McFarland nephelometer to assess...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tuberculosis; PCR.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900003

Comparison of scientists of the Brazilian Academy of Sciences and of the National Academy of Sciences of the USA on the basis of the h-index
Provedor de dados: 56
Autores: Mugnaini,R.; Packer,A.L.; Meneghini,R.
A new scientometric indicator, the h-index, has been recently proposed (Hirsch JE. Proc Natl Acad Sci 2005; 102: 16569-16572). The index avoids some shortcomings of the calculation of the total number of citations as a parameter to evaluate scientific performance. Although it has become known only recently, it has had widespread acceptance. A comparison of the average h-index of members of the Brazilian Academy of Sciences (BAS) and of the National Academy of Sciences of the USA (NAS-USA) was carried out for 10 different areas of science. Although, as expected, the comparison was unfavorable to the members of the BAS, the imbalance was distinct in different areas. Since these two academies represent, to a significant extent, the science of top quality...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Scientometrics; Bibliometrics; Index h; Science evaluation; Scientific academy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400001

Decreased expression of chromodomain helicase DNA-binding protein 9 is a novel independent prognostic biomarker for colorectal cancer
Provedor de dados: 56
Autores: Xu,Li; Peng,Hui; Huang,Xiao-Xu; Xia,Ya-Bin; Hu,Kai-Feng; Zhang,Zheng-Ming.
Previous studies suggested that chromodomain helicase DNA-binding proteins (CHDs), including CHD 1–8, were associated with several human diseases and cancers including lymphoma, liver cancer, colorectal cancer, stomach cancer, etc. To date, little research on CHD 9 in human cancers has been reported. In this study, we assessed the prognostic value of CHD 9 in patients with colorectal cancer (CRC). We screened for CHD 9 expression using immunohistochemical analysis in 87 surgical CRC specimens and found that the expression was upregulated in 81.5% of the cases, while 7.4% were decreased; in the remaining 11.1% of the cases, levels were not altered. Kaplan-Meier analysis showed that patients with high CHD 9 expression had better prognosis than those with low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromodomain helicase DNA-binding protein 9; Colorectal cancer; Immunohistochemical analysis; Biomarker; Prognosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900612

A conceptual and practical overview of cDNA microarray technology: implications for basic and clinical sciences
Provedor de dados: 56
Autores: Mello-Coelho,V. de; Hess,K.L.
cDNA microarray is an innovative technology that facilitates the analysis of the expression of thousands of genes simultaneously. The utilization of this methodology, which is rapidly evolving, requires a combination of expertise from the biological, mathematical and statistical sciences. In this review, we attempt to provide an overview of the principles of cDNA microarray technology, the practical concerns of the analytical processing of the data obtained, the correlation of this methodology with other data analysis methods such as immunohistochemistry in tissue microarrays, and the cDNA microarray application in distinct areas of the basic and clinical sciences.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotechnology; CDNA microarray; Gene expression; Genomics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000011

Circadian cardiac autonomic function in perinatally HIV-infected preschool children
Provedor de dados: 56
Autores: Benchimol-Barbosa,P.R.
The 24-h heart rate variability and QT-interval adaptation was investigated in perinatally HIV-infected preschool children classified according to immunological status in order to assess autonomic function at early stages of infection. Thirty-five perinatally HIV-infected and clinically stable children (4.8 ± 0.3 years) were enrolled after approval of the study by the University Hospital Pedro Ernesto Ethics Committee and written informed parental consent was obtained. The children were classified according to peripheral CD4+ count (cells/µL) as follows: group 1, N = 11 (≥1000); group 2, N = 7 (≥500 and &lt;1000); group 3, N = 17 (&lt;500). Left ventricular ejection fraction (&gt;55%), 24-h RR interval variability (RRV) indexes (NN, SDANN, SDNN index,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autonomic nervous system; Circadian rhythm; Heart rate variability; Human immunodeficiency virus infection; Perinatally HIV-infected children.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800006

Comparative efficacy and safety of the left versus right radial approach for percutaneous coronary procedures: a meta-analysis including 6870 patients
Provedor de dados: 56
Autores: Xia,S.L.; Zhang,X.B.; Zhou,J.S.; Gao,X.
The radial approach is widely used in the treatment of patients with coronary artery disease. We conducted a meta-analysis of published results on the efficacy and safety of the left and right radial approaches in patients undergoing percutaneous coronary procedures. A systematic search of reference databases was conducted, and data from 14 randomized controlled trials involving 6870 participants were analyzed. The left radial approach was associated with significant reductions in fluoroscopy time [standardized mean difference (SMD)=-0.14, 95% confidence interval (CI)=-0.19 to -0.09; P&lt;0.00001] and contrast volume (SMD=-0.07, 95%CI=-0.12 to -0.02; P=0.009). There were no significant differences in rate of procedural failure of the left and the right...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radial approach; Percutaneous coronary; Meta-analysis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800743

In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
Provedor de dados: 56
Autores: Isa,N.M.; Abdelwahab,S.I.; Mohan,S.; Abdul,A.B.; Sukari,M.A.; Taha,M.M.E.; Syam,S.; Narrima,P.; Cheah,S.Ch.; Ahmad,S.; Mustafa,M.R.
The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell lung cancer (A549), prostate adenocarcinoma (PC3), and normal hepatic cells (WRL-68) were used to evaluate the cytotoxicity of BA using the MTT assay. The antioxidant activity of BA was assessed by the ORAC assay and compared to quercetin as a standard reference antioxidant. ORAC results are reported as the equivalent concentration of Trolox that produces the same level of antioxidant activity as the sample tested at 20 µg/mL. The toxic effect of BA on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Boesenbergia rotunda; Boesenbergin A; Cytotoxicity; Antioxidation; Anti-inflammatory.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600008

Daily birth numbers in Passo Fundo, South Brazil, 1997-1999: trends and periodicities
Provedor de dados: 56
Autores: Mikulecky,M.; Lisboa,H.R.K.
Between October 6, 1997 and April 30, 1999, 5011 births (mean: 8.76 per day) were registered in the city of Passo Fundo, South Brazil. The sequence of 572 daily birth numbers was not random (iteration test). Neyman distribution (m = ¥) showed the best fit. Clusters of days with higher birth numbers alternated with days with low numbers of births. Periodogram analysis revealed a significant periodicity of 6.98 days. The cosinor regression, testing 10 a priori supposed period lengths, found significant seasonality peaking in August-September and significantly highest birth numbers on Thursdays. Among the lunar and solar rotation cycles, the tropic lunar cycle and its 4th harmonic were most pronounced, in agreement with results concerning natality in Germany...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Births; South Brazil; Periodogram; Cosinor; Seasonality; Weekly rhythm; Multiseptans; Cosmogeophysical-biomedical parallelism; Solar and lunar cycles.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800017

Down-regulated miR-448 relieves spinal cord ischemia/reperfusion injury by up-regulating SIRT1
Provedor de dados: 56
Autores: Wang,Yun; Pang,Qing-Jiang; Liu,Jiang-Tao; Wu,Hai-Hao; Tao,Dong-Ying.
MicroRNAs play a crucial role in the progression of spinal cord ischemia/reperfusion injury (SCII). The role of miR-448 and SIRT1 in SCII was investigated in this study, to provide further insights into prevention and improvement of this disorder. In this study, expressions of miR-448 and SIRT1 protein were determined by qRT-PCR and western blot, respectively. Flow cytometry was used to analyze cell apoptosis. The endogenous expression of genes was modulated by recombinant plasmids and cell transfection. Dual-luciferase reporter assay was performed to determine the interaction between miR-448 and SIRT1. The Basso, Beattie, and Bresnahan score was used to measure the hind-limb function of rat. The spinal cord ischemia reperfusion injury model of adult rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SCII; MiR-448; SIRT1; Nerve cells.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500610

Implications of extubation failure and prolonged mechanical ventilation in the postoperative period following elective intracranial surgery
Provedor de dados: 56
Autores: Vidotto,M.C.; Sogame,L.C.; Gazzotti,M.R.; Prandini,M.; Jardim,J.R.
Patients undergoing neurosurgery are predisposed to a variety of complications related to mechanical ventilation (MV). There is an increased incidence of extubation failure, pneumonia, and prolonged MV among such patients. The aim of the present study was to assess the influence of extubation failure and prolonged MV on the following variables: postoperative pulmonary complications (PPC), mortality, reoperation, tracheostomy, and duration of postoperative hospitalization following elective intra-cranial surgery. The study involved a prospective observational cohort of 317 patients submitted to elective intracranial surgery for tumors, aneurysms and arteriovenous malformation. Preoperative assessment was performed and patients were followed up for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurosurgery; Craniotomy; Ventilator weaning; Intratracheal intubation; Postoperative period.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200014

Fighting by sleep-deprived rats as a possible manifestation of panic: effects of sodium lactate
Provedor de dados: 56
Autores: Furlan,F.A.; Hoshino,K.
Increased fighting is an effect of desynchronized sleep deprivation (DSD) in rats, and recently this behavior has been suggested to be spontaneous panic and equivalent to panic disorder. In the present study we tested this hypothesis by evaluating the effect of sodium lactate on this aggressiveness, because this substance is recognized to induce spontaneous panic attacks in patients. A total of 186 male albino Wistar rats, 250-350 g, 90-120 days of age, were submitted to DSD (multiple platform method) for 0, 4, or 5 days. At the end of the deprivation period the rats were divided into subgroups respectively injected intraperitoneally with 1.86, 2.98 and 3.72 g/kg of 1 M sodium lactate, or 1.86 and 3.72 g/kg of 2 M sodium lactate. The control animals were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat; Sleep deprivation; Panic; Aggressiveness; Sodium lactate.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300010

Influences of alpha-tocopherol on cholesterol metabolism and fatty streak development in apolipoprotein E-deficient mice fed an atherogenic diet
Provedor de dados: 56
Autores: Peluzio,M.C.G.; Homem,A.P.P.; Cesar,G.C.; Azevedo,G.S.; Amorim,R.; Cara,D.C.; Saliba,H.; Vieira,E.C.; Arantes,R.E.; Alvarez-Leite,J.
Although the role of oxidized lipoproteins is well known in atherogenesis, the role of vitamin E supplementation is still controversial. There is also little information about cholesterol metabolism (hepatic concentration and fecal excretion) in the new models of atherosclerosis. In the present study, we evaluated the effect of moderate vitamin E supplementation on cholesterol metabolism and atherogenesis in apolipoprotein E (apo E)-deficient mice. Apo E-deficient mice were fed an atherogenic diet containing 40 or 400 mg/kg of alpha-tocopherol acetate for 6 weeks. Total cholesterol in serum and liver and 3-OH-alpha-sterols in feces, and fecal excretion of bile acids were determined and histological analyses of aortic lesion were performed. A vitamin E-rich...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Atherosclerosis; Apo E; Mice; Alpha-tocopherol; Cholesterol.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200005

Psychological stress alters the ultrastructure and increases IL-1β and TNF-α in mandibular condylar cartilage
Provedor de dados: 56
Autores: Lv,Xin; Li,Qiang; Wu,Shun; Sun,Jing; Zhang,Min; Chen,Yong-Jin.
Psychological factors can be correlated with temporomandibular disorders (TMDs), but the mechanisms are unknown. In the present study, we examined the microstructural changes and expression of proinflammatory cytokines in mandibular condylar cartilage of the temporomandibular joint (TMJ) in a psychological stress animal model. Male Sprague-Dawley rats (8 weeks old, 210 ± 10 g) were randomly divided into 3 groups: psychological stress (PS, N = 48), foot shock (FS, N = 24), and control (N = 48). After inducing psychological stress using a communication box with the FS rats for 1, 3, or 5 weeks, PS rats were sacrificed and compared to their matched control littermates, which received no stress and were killed at the same times as the PS rats. Body and adrenal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psychological stress; Temporomandibular joint; IL-1β; TNF-α.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000013

Ischemia preconditioning is neuroprotective in a rat cerebral ischemic injury model through autophagy activation and apoptosis inhibition
Provedor de dados: 56
Autores: Xia,D.Y.; Li,W.; Qian,H.R.; Yao,S.; Liu,J.G.; Qi,X.K.
Sublethal ischemic preconditioning (IPC) is a powerful inducer of ischemic brain tolerance. However, its underlying mechanisms are still not well understood. In this study, we chose four different IPC paradigms, namely 5 min (5 min duration), 5×5 min (5 min duration, 2 episodes, 15-min interval), 5×5×5 min (5 min duration, 3 episodes, 15-min intervals), and 15 min (15 min duration), and demonstrated that three episodes of 5 min IPC activated autophagy to the greatest extent 24 h after IPC, as evidenced by Beclin expression and LC3-I/II conversion. Autophagic activation was mediated by the tuberous sclerosis type 1 (TSC1)-mTor signal pathway as IPC increased TSC1 but decreased mTor phosphorylation. Terminal deoxynucleotidyl transferase dUTP nick end...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ischemic preconditioning; Autophagy; Apoptosis; Focal cerebral ischemic reperfusion.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700580

Effect of age and gender on sweat lactate and ammonia concentrations during exercise in the heat
Provedor de dados: 56
Autores: Meyer,F.; Laitano,O.; Bar-Or,O.; McDougall,D.; Heigenhauser,G.J.F.
The dependence of sweat composition and acidity on sweating rate (SR) suggests that the lower SR in children compared to adults may be accompanied by a higher level of sweat lactate (Lac-) and ammonia (NH3) and a lower sweat pH. Four groups (15 girls, 18 boys, 8 women, 8 men) cycled in the heat (42ºC, 20% relative humidity) at 50% VO2max for two 20-min bouts with a 10-min rest before bout 1 and between bouts. Sweat was collected into plastic bags attached to the subject's lower back. During bout 1, sweat from girls and boys had higher Lac- concentrations (23.6 ± 1.2 and 21.2 ± 1.7 mM; P &lt; 0.05) than sweat from women and men (18.2 ± 1.9 and 14.8 ± 1.6 mM, respectively), but Lac- was weakly associated with SR (P &gt; 0.05; r = -0.27). Sweat Lac-...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sweat pH; Electrolytes; Ammonia; Lactate.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100017

DNA-lipid systems: A physical chemistry study
Provedor de dados: 56
Autores: Dias,R.; Antunes,F.; Miguel,M.; Lindman,S.; Lindman,B.
It is well known that the interaction of polyelectrolytes with oppositely charged surfactants leads to an associative phase separation; however, the phase behavior of DNA and oppositely charged surfactants is more strongly associative than observed in other systems. A precipitate is formed with very low amounts of surfactant and DNA. DNA compaction is a general phenomenon in the presence of multivalent ions and positively charged surfaces; because of the high charge density there are strong attractive ion correlation effects. Techniques like phase diagram determinations, fluorescence microscopy, and ellipsometry were used to study these systems. The interaction between DNA and catanionic mixtures (i.e., mixtures of cationic and anionic surfactants) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Cationic surfactants; Catanionic mixtures; Chitosan; Phase behavior; Polyelectrolyte-oppositely charged surfactant systems.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500002

Impact of a 2-year intervention program on cardiometabolic profile according to the number of goals achieved
Provedor de dados: 56
Autores: Almeida-Pittito,B.; Hirai,A.T.; Sartorelli,D.S.; Gimeno,S.G.A.; Ferreira,S.R.G.
We investigated the impact of lifestyle goal achievement on cardiovascular risk factors after a 2-year behavioral intervention program applied to 394 adults (113 with diabetes, mean age 60.2 ± 11.4 years, 56% women) and targeting four goals: ≥5% weight loss; ≥150 min/week physical activities; &lt;10% saturated fat intake/day; ≥400 g fruit and vegetable intake/day. Baseline characteristics and changes in variables after intervention among the four categories of number of goals achieved (none, 1, 2, and ≥3) were compared by independent ANOVA or the Kruskal-Wallis test. Individuals without diabetes achieving a higher number of goals were more likely to be older (3 or 4 goals: 61.8 ± 12.6 years vs none: 53.3 ± 10.3 years, P &lt; 0.05) and to have a lower mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Behavioral intervention; Lifestyle; Goals; Glucose metabolism disturbance; Cardiovascular risk factor.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100011

Mini-Mu insertions in the tetracycline resistance determinant from Proteus mirabilis
Provedor de dados: 56
The inducible tetracycline resistance determinant isolated from Proteus mirabilis cloned into the plasmid pACYC177 was mutagenized by insertion of a mini-Mu-lac phage in order to define the regions in the cloned sequences encoding the structural and regulatory proteins. Three different types of mutants were obtained: one lost the resistance phenotype and became Lac+; another expressed the resistance at lower levels and constitutively; the third was still dependent on induction but showed a lower minimal inhibitory concentration. The mutant phenotypes and the locations of the insertions indicate that the determinant is composed of a repressor gene and a structural gene which are not transcribed divergently as are other known tetracycline determinants...
Palavras-chave: Tetracycline; Mutation; LacZ-fusion; Proteus mirabilis; Bacteriophage Mu.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300009

Involvement of the hippocampus, amygdala, entorhinal cortex and posterior parietal cortex in memory consolidation
Provedor de dados: 56
A total of 182 young adult male Wistar rats were bilaterally implanted with cannulae into the CA1 region of the dorsal hippocampus and into the amygdaloid nucleus, the entorhinal cortex, and the posterior parietal cortex. After recovery, the animals were trained in a step-down inhibitory avoidance task. At various times after training (0, 30, 60 or 90 min) the animals received a 0.5-µl microinfusion of vehicle (saline) or 0.5 µg of muscimol dissolved in the vehicle. A retention test was carried out 24 h after training. Retention test performance was hindered by muscimol administered into both the hippocampus and amygdala at 0 but not at 30 min posttraining. The drug was amnestic when given into the entorhinal cortex 30, 60 or 90 min after training, or into...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Memory consolidation; Hippocampus and amygdala; Entorhinal cortex; Parietal cortex.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200012

The Agamma-195 (C-&gt;G) mutation in hereditary persistence of fetal hemoglobin is not associated with activation of a reporter gene in vitro
Provedor de dados: 56
Autores: Schreiber,R.; Gonçalves,M.S.; Junqueira,M.L.; Saad,S.T.O.; Krieger,J.E.; Costa,F.F.
Hereditary persistence of fetal hemoglobin is an uncommon, benign disorder in which the expression of gamma-globin genes persists into adult life. Several point mutations have been associated with the increased gamma-globin gene promoter activity. We evaluated the -195 (C-&gt;G) mutation by a functional in vitro assay based on the luciferase reporter gene system. The results indicated that the increased promoter activity observed in vivo could not be reproduced in vitro under the conditions employed, suggesting that other factors may be involved in the overexpression of the gamma-globin gene containing the -195 (C-&gt;G) mutation. Furthermore, this is the first time that the -195 (C-&gt;G) mutation of the Agamma-globin gene has been evaluated by in vitro...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fetal hemoglobin; Hereditary persistence of fetal hemoglobin; HPFH; Transient expression.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400008

Collagens and proteoglycans of the corneal extracellular matrix
Provedor de dados: 56
Autores: Michelacci,Y.M.
The cornea is a curved and transparent structure that provides the initial focusing of a light image into the eye. It consists of a central stroma that constitutes 90% of the corneal depth, covered anteriorly with epithelium and posteriorly with endothelium. Its transparency is the result of the regular spacing of collagen fibers with remarkably uniform diameter and interfibrillar space. Corneal collagen is composed of heterotypic fibrils consisting of type I and type V collagen molecules. The cornea also contains unusually high amounts of type VI collagen, which form microfibrillar structures, FACIT collagens (XII and XIV), and other nonfibrillar collagens (XIII and XVIII). FACIT collagens and other molecules, such as leucine-rich repeat proteoglycans,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cornea; Proteoglycan; Glycosaminoglycan; Collagen; Dermatan sulfate; Keratan sulfate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800009

Poor sleep in middle-aged women is not associated with menopause per se
Provedor de dados: 56
Autores: Tao,M.F.; Sun,D.M.; Shao,H.F.; Li,C.B.; Teng,Y.C.
Whether sleep problems of menopausal women are associated with vasomotor symptoms and/or changes in estrogen levels associated with menopause or age-related changes in sleep architecture is unclear. This study aimed to determine if poor sleep in middle-aged women is correlated with menopause. This study recruited women seeking care for the first time at the menopause outpatient department of our hospital. Inclusion criteria were an age ≥40 years, not taking any medications for menopausal symptoms, and no sleeping problems or depression. Patients were assessed with the Pittsburgh Sleep Quality Index (PSQI), modified Kupperman Index (KI), and Menopause Rating Scale (MRS). A PSQI score of &lt;7 indicated no sleep disorder and ≥7 indicated a sleep disorder....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kupperman Index; Menopause; Menopause Rating Scale; Pittsburgh Sleep Quality Index; Sleep disorder; Quality of life.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100701

Abnormalities of hippocampal signal intensity in patients with familial mesial temporal lobe epilepsy
Provedor de dados: 56
Autores: Coan,A.C.; Kobayashi,E.; Lopes-Cendes,I.; Li,L.M.; Cendes,F.
Mesial temporal lobe epilepsy (MTLE) is associated with hippocampal atrophy and hippocampal signal abnormalities. In our series of familial MTLE (FMTLE), we found a high proportion of hippocampal abnormalities. To quantify signal abnormalities in patients with FMTLE we studied 152 individuals (46 of them asymptomatic) with FMTLE. We used NIH-Image® for volumetry and signal quantification in coronal T1 inversion recovery and T2 for all cross-sections of the hippocampus. Values diverging by 2 or more SD from the control mean were considered abnormal. T2 hippocampal signal abnormalities were found in 52% of all individuals: 54% of affected subjects and 48% of asymptomatic subjects. T1 hippocampal signal changes were found in 34% of all individuals: 42.5% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hippocampal signal abnormalities; Familial mesial temporal lobe epilepsy; Hippocampal volumetry; Magnetic resonance imaging.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600007

Cytokine profile and nitric oxide levels in sera from patients with brucellosis
Provedor de dados: 56
Autores: Refik,M.; Mehmet,N.; Durmaz,R.; Ersoy,Y.
The aims of this study were to investigate the serum levels of some cytokines [tumor necrosis factor-alpha (TNF-alpha), interleukin 1ß (IL-1ß), IL-2R, IL-6, and IL-8] and nitric oxide (NO) levels in patients with untreated brucellosis and to test the correlation of these parameters with each other. The study was conducted on 67 subjects, 37 patients with brucellosis and 30 healthy individuals with no history of Brucella infection. Brucellosis was identified by a positive blood culture and/or increased Brucella antibodies in serological tests in addition to compatible clinical symptoms. Cytokine profile analysis was performed by the immulite chemiluminescent enzyme immunometric assay whose inter- and intra-assay coefficients of variance were 2.6-3.6 and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Human brucellosis; Cytokines; Nitric oxide; Interleukin-2; Interleukin-6; Interleukin-8.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100010

Differences in functional and structural properties of segments of the rat tail artery
Provedor de dados: 56
Autores: Souza,F.M.; Padilha,A.S.; Stefanon,I.; Vassallo,D.V.
The investigation of resistance vessels is generally costly and difficult to execute. The present study investigated the diameters and the vascular reactivity of different segments of the rat tail artery (base, middle, and tail end) of 30 male Wister rats (EPM strain) to characterize a conductance or resistance vessel, using a low-cost simple technique. The diameters (mean ± SEM) of the base and middle segments were 471 ± 4.97 and 540 ± 8.39 µm, respectively, the tail end was 253 ± 2.58 µm. To test reactivity, the whole tail arteries or segments were perfused under constant flow and the reactivity to phenylephrine (PHE; 0.01-300 µg) was evaluated before and after removal of the endothelium or drug administration. The maximal response (Emax) and sensitivity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat tail artery; Phenylephrine; Resistance artery; Endothelium; Conductance artery.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500011

Aging reduces the primary humoral response and the in vitro cytokine production in mice
Provedor de dados: 56
Autores: Simioni,P.U.; Costa,E.H.; Tamashiro,W.M.S.C.
Aging is accompanied by a decrease in several physiological functions that make older individuals less responsive to environmental challenges. In the present study, we analyzed the immune response of female BALB/c mice (N = 6) of different ages (from 2 to 96 weeks) and identified significant age-related alterations. Immunization with hapten-protein (trinitrophenyl-bovine serum albumin) conjugates resulted in lower antibody levels in the primary and secondary responses of old mice (72 weeks old). Moreover, young mice (2, 16, and 32 weeks old) maintained specific antibodies in their sera for longer periods after primary immunization than did old mice. However, a secondary challenge efficiently induced memory in old mice, as shown by the increased antibody...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Cytokines; Antibody; Proliferation; T cell response; T cell subsets.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800012

Caspase dependence of the death of neonatal retinal ganglion cells induced by axon damage and induction of autophagy as a survival mechanism
Provedor de dados: 56
Autores: Sternberg,C.; Benchimol,M.; Linden,R.
We examined the degeneration of post-mitotic ganglion cells in ex-vivo neonatal retinal explants following axon damage. Ultrastructural features of both apoptosis and autophagy were detected. Degenerating cells reacted with antibodies specific for activated caspase-3 or -9, consistent with the presence of caspase activity. Furthermore, peptidic inhibitors of caspase-9, -6 or -3 prevented cell death (100 µM Ac-LEDH-CHO, 50 µM Ac-VEID-CHO and 10 µM Z-DEVD-fmk, respectively). Interestingly, inhibition of autophagy by 7-10 mM 3-methyl-adenine increased the rate of cell death. Immunohistochemistry data, caspase activation and caspase inhibition data suggest that axotomy of neonatal retinal ganglion cells triggers the intrinsic apoptotic pathway, which, in turn,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Autophagy; Caspases; Retina; Central nervous system.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000005

Effects of isoflavone on the learning and memory of women in menopause: a double-blind placebo-controlled study
Provedor de dados: 56
Autores: Santos-Galduróz,R.F.; Galduróz,J.C.F.; Facco,R.L.; Hachul,H.; Tufik,S.
Hormone decline is common to all women during aging and, associated with other factors, leads to cognitive impairment. Its replacement enhances cognitive performance, but not all women present a clinical and family or personal history that justifies its use, mainly women with a history of cancer. The aim of this study was to determine whether a daily oral dose of 80 mg of isoflavone extract for 4 months can produce benefits in women with low hormone levels, contributing to improvement in cognitive aspects. The sample comprised 50- to 65-year-old women whose menstruation had ceased at least 1 year before and who had not undergone hormone replacement. The volunteers were allocated to two groups of 19 individuals each, i.e., isoflavone and placebo. There was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isoflavone; Cognitive disorders; Aging; Information integration; Memory; Menopause.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100016

Evaluation of genotype resistance testing for salvage antiretroviral therapy at AIDS care centers from Ribeirão Preto, São Paulo, Brazil
Provedor de dados: 56
Autores: Pires Neto,R.J.; Colares,J.K.B.; Fonseca,B.A.L.
The availability of HIV-1 genotype resistance testing (GRT) to clinicians has been insufficiently studied outside randomized clinical trials. The present study evaluated the outcome of salvage antiretroviral therapy (ART) recommended by an expert physician based on GRT in a non-clinical trial setting in Ribeirão Preto, Brazil. A prospective, open, nonrandomized study evaluating easy access to GRT at six Brazilian AIDS Clinics was carried out. This cooperative study analyzed the efficacy of treatment recommended to patients whose salvage ART was guided by GRT with that of treatment with ART based only on previous ART history. A total of 112 patients with ART failure were included in the study, and 77 of them were submitted to GRT. The median CD4 cell count...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; Antiretroviral therapy; Salvage therapy; HIV-1 genotype resistance testing.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600016

Inflammatory and oxidative stress airway markers in premature newborns of hypertensive mothers
Provedor de dados: 56
Autores: Madoglio,R.J.; Rugolo,L.M.S.S.; Kurokawa,C.S.; Sá,M.P.A.; Lyra,J.C.; Antunes,L.C.O.
Although oxidative stress and inflammation are important mechanisms in the pathophysiology of preeclampsia and preterm diseases, their contribution to the respiratory prognosis of premature infants of hypertensive mothers is not known. Our objective was to determine the levels of oxidative stress and inflammation markers in the airways of premature infants born to hypertensive and normotensive mothers, in the first 72 h of life, and to investigate whether they are predictors of bronchopulmonary dysplasia (BPD)/death. This was a prospective study with premature infants less than 34 weeks’ gestation on respiratory support who were stratified into 2 groups: 32 premature infants of hypertensive mothers and 41 of normotensive women, with a mean gestational age...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infant; Premature; Pre-eclampsia; Oxidative stress; Cytokines; Bronchopulmonary dysplasia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900703

Comparison of three methods for the determination of baroreflex sensitivity in conscious rats
Provedor de dados: 56
Autores: Farah,V.M.A.; Moreira,E.D.; Pires,M.D.; Irigoyen,M.C.C.; Krieger,E.M.
Baroreflex sensitivity was studied in the same group of conscious rats using vasoactive drugs (phenylephrine and sodium nitroprusside) administered by three different approaches: 1) bolus injection, 2) steady-state (blood pressure (BP) changes produced in steps), 3) ramp infusion (30 s, brief infusion). The heart rate (HR) responses were evaluated by the mean index (mean ratio of all HR changes and mean arterial pressure (MAP) changes), by linear regression and by the logistic method (maximum gain of the sigmoid curve by a logistic function). The experiments were performed on three consecutive days. Basal MAP and resting HR were similar on all days of the study. Bradycardic responses evaluated by the mean index (-1.5 ± 0.2, -2.1 ± 0.2 and -1.6 ± 0.2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baroreflex; Baroreceptor; Bradycardia; Tachycardia.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300018

Urinary NO3 excretion and renal failure in indinavir-treated patients
Provedor de dados: 56
Autores: Eira,M.; Araujo,M.; Seguro,A.C.
Treatment with indinavir (IDV), a protease inhibitor, is frequently associated with renal abnormalities. We determined the incidence of renal failure (creatinine clearance &lt;80 mL min-1 1.73 (m²)-1) in HIV patients treated with highly active antiretroviral therapy, including IDV, and investigated the possible mechanisms and risk factors of IDV nephrotoxicity. Thirty-six patients receiving IDV were followed for 3 years. All were assessed for age, body weight, duration of infection, duration of IDV treatment, sulfur-derivative use, total cholesterol, triglycerides, magnesium, sodium, potassium, creatinine, and urinalysis. We also determined renal function in terms of creatinine clearance, urine osmolality and fractional excretion of sodium, potassium, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Indinavir; Nephrotoxicity; Nitric oxide.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800009

Effect of thyroparathyroidectomy on urinary acidification in diabetic rats
Provedor de dados: 56
Autores: Zaladek-Gil,F.; Cavanal,M.F.; Nascimento-Gomes,G.; Silva-Rocha,M.C.; Nutti-de-Almeida,M.
In previous studies we have shown stimulation of renal acid excretion in the proximal tubules of rats with diabetes of short duration, with no important alterations in glomerular hemodynamics; on the other hand, in thyroparathyroidectomized rats (TPTX model), a significant decrease in renal acid excretion, glomerular filtration rate (GFR) and renal plasma flow (RPF) was detected. Since important changes in the parathyroid hormone-vitamin D-Ca axis are observed in the diabetic state, the present study was undertaken to investigate the renal repercussions of thyroparathyroidectomy in rats previously made diabetic by streptozotocin (45 mg/kg). Four to 6 days after the induction of diabetes (DM), a group of rats were thyroparathyroidectomized (DM + TPTX)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Thyroparathyroidectomy; Renal function; Acid excretion.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100016

Observation of the effect of bone marrow mesenchymal stem cell transplantation by different interventions on cirrhotic rats
Provedor de dados: 56
Autores: Zhou,Xiaoling; Yang,Jianqing; Liu,Ying; Li,Zepeng; Yu,Jingfang; Wei,Wanhua; Chen,Qiao; Li,Can; Tang,Nong.
Bone marrow mesenchymal stem cells (BMSCs) transplantation has attracted attention for the treatment of liver cirrhosis and end-stage liver diseases. Therefore, in this study, we evaluated the effect of different methods of BMSCs transplantation in the treatment of liver cirrhosis in rats. Seventy-two male Sprague-Dawley rats were divided into 7 groups: 10 were used to extract BMSCs, 10 were used as normal group, and the remaining 52 rats were randomly divided into five groups for testing: control group, BMSCs group, BMSCs+granulocyte colony-stimulating factor (G-CSF) group, and BMSCs+Jisheng Shenqi decoction (JSSQ) group. After the end of the intervention course, liver tissue sections of rats were subjected to hematoxylin and eosin (H&amp;E) and Masson...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow mesenchymal stem cells; Transplantation; Liver cirrhosis; Granulocyte colony-stimulating factor; Jisheng Shenqi decoction.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300606

Gap junctions in cells of the immune system: structure, regulation and possible functional roles
Provedor de dados: 56
Autores: Sáez,J.C.; Brañes,M.C.; Corvalán,L.A.; Eugenín,E.A.; González,H.; Martínez,A.D.; Palisson,F.
Gap junction channels are sites of cytoplasmic communication between contacting cells. In vertebrates, they consist of protein subunits denoted connexins (Cxs) which are encoded by a gene family. According to their Cx composition, gap junction channels show different gating and permeability properties that define which ions and small molecules permeate them. Differences in Cx primary sequences suggest that channels composed of different Cxs are regulated differentially by intracellular pathways under specific physiological conditions. Functional roles of gap junction channels could be defined by the relative importance of permeant substances, resulting in coordination of electrical and/or metabolic cellular responses. Cells of the native and specific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell contacts; Gap junctions; Connexins; Native immune response; Specific immune response; Inflammatory response.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400011

Effect of activation of canonical Wnt signaling by the Wnt-3a protein on the susceptibility of PC12 cells to oxidative and apoptotic insults
Provedor de dados: 56
Autores: Kawamoto,E.M.; Gleichmann,M.; Yshii,L.M.; Lima,L. de Sá; Mattson,M.P.; Scavone,C.
Wnt proteins are involved in tissue development and their signaling pathways play an important role during embryogenesis. Wnt signaling can promote cell survival, which is beneficial for neurons, but could also lead to tumor development in different tissues. The present study investigated the effects of a Wnt protein on the susceptibility of a neural tumor cell line (PC12 cells) to the cytotoxic compounds ferrous sulfate (10 mM), staurosporine (100 and 500 nM), 3-nitropropionic acid (5 mM), and amyloid β-peptide (Aβ25-35; 50 µM). Cells (1 x 10(6) cells/mL) were treated with the Wnt-3a recombinant peptide (200 ng/mL) for 24 h before exposure to toxic insults. The Wnt-3a protein partially protected PC12 cells, with a 6-15% increase in cell viability in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wnt-3a; PC12 cells; Beta-catenin; NF-κB.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100010

microRNA-142-3p inhibits apoptosis and inflammation induced by bleomycin through down-regulation of Cox-2 in MLE-12 cells
Provedor de dados: 56
Autores: Guo,F.; Lin,S.C.; Zhao,M.S.; Yu,B.; Li,X.Y.; Gao,Q.; Lin,D.J.
microRNA (miR)-142-3p is implicated in malignancy and has been identified as a biomarker for aggressive and recurrent lung adenocarcinomas. This study aimed to evaluate the inhibitory effect of miR-142-3p on apoptosis and inflammation induced by bleomycin in MLE-12 cells. MLE-12 cells were first transfected either with miR-142-3p mimic or miR-142-3p inhibitor and then the cells were exposed to 50 μg/mL of bleomycin. Thereafter, cell viability, apoptosis and the expression of pro-inflammatory cytokines were assessed using CCK-8, flow cytometry, RT-PCR and western blot analyses. Cox-2, PI3K, AKT and mTOR expressions were detected by western blotting after bleomycin was administered together with NS-398 (an inhibitor of Cox-2). As a result, cell viability was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-142-3p; MLE-12 cells; Bleomycin; Apoptosis; Pro-inflammatory cytokines; Idiopathic pulmonary fibrosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700606

Dissociation between the circulating renin-angiotensin system and angiotensin II receptors in central losartan-induced hypertension
Provedor de dados: 56
Autores: Sugawara,A.M.; Vendramini,R.C.; Barbosa,S.P.; Brunetti,I.L.; Menani,J.V.; De Luca Jr.,L.A.
Losartan, an AT1 angiotensin II (ANG II) receptor non-peptide antagonist, induces an increase in mean arterial pressure (MAP) when injected intracerebroventricularly (icv) into rats. The present study investigated possible effector mechanisms of the increase in MAP induced by icv losartan in unanesthetized rats. Male Holtzman rats (280-300 g, N = 6/group) with a cannula implanted into the anterior ventral third ventricle received an icv injection of losartan (90 µg/2 µl) that induced a typical peak pressor response within 5 min. In one group of animals, this response to icv losartan was completely reduced from 18 ± 1 to 4 ± 2 mmHg by intravenous (iv) injection of losartan (2.5-10 mg/kg), and in another group, it was partially reduced from 18 ± 3 to 11 ± 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; Alpha1-Adrenoceptors; Arterial pressure; AT1 receptors; Angiotensin-converting enzyme; Renin.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900007

A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media
Provedor de dados: 56
Autores: Schneedorf,J.M.; Santoro,M.M.; Lovrien,R.E.; Mares-Guia,M.
Synthetic dyes bind to proteins causing selective coprecipitation of the complexes in acid aqueous solution by a process of reversible denaturation that can be used as an alternative method for protein fractionation. The events that occur before precipitation were investigated by equilibrium dialysis using bovine trypsin and flavianic acid as a model able to cause coprecipitation. A two-step mode of interaction was found to be dependent on the incubation periods allowed for binding, with pronounced binding occurring after 42 h of incubation. The first step seems to involve hydration effects and conformational changes induced by binding of the first dye molecule, following rapid denaturation due to the binding of six additional flavianate anions to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dye binding; Binding isotherm; Coprecipitation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800012

Lung tissue mechanics in the early stages of induced paracoccidioidomycosis in rats
Provedor de dados: 56
Autores: Shikanai-Yasuda,M.A.; Pereira,P.M.; Yamashiro-Kanashiro,E.; Duarte,M.I.S.; Assis,C.M.; Geraldes,E.A.; Saldiva,P.H.N.
Pulmonary dysfunction represents the most important cause of death in patients with paracoccidioidomycosis (PBM). In order to investigate the functional changes of the lungs in the early stages of PBM, a model of benign disease was developed by intratracheal challenge of 12-week old isogenic Wistar rats with 1 x 106 yeast forms of Paracoccidioides brasiliensis. Animals were studied 30 and 60 days after infection, when fully developed granulomas were demonstrable in the lungs. Measurements of airway resistance, lung elastance and tissue hysteresis were made during sinusoidal deformations (100 breaths/min, tidal volume = 2 ml) with direct measurement of alveolar pressure using the alveolar capsule technique. Infection caused a significant increase in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental paracoccidioidomycosis; Pulmonary mechanics; Tissue mechanics; Alveolar pressure measurements; Hysteresis.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000006

Contrasting effects of nitric oxide and corticotropin- releasing factor within the dorsal periaqueductal gray on defensive behavior and nociception in mice
Provedor de dados: 56
Autores: Miguel,T.T.; Gomes,K.S.; Nunes-de-Souza,R.L.
The anxiogenic and antinociceptive effects produced by glutamate N-methyl-D-aspartate receptor activation within the dorsal periaqueductal gray (dPAG) matter have been related to nitric oxide (NO) production, since injection of NO synthase (NOS) inhibitors reverses these effects. dPAG corticotropin-releasing factor receptor (CRFr) activation also induces anxiety-like behavior and antinociception, which, in turn, are selectively blocked by local infusion of the CRF type 1 receptor (CRFr1) antagonist, NBI 27914 [5-chloro-4-(N-(cyclopropyl)methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl)aminopyridine]. Here, we determined whether i) the blockade of the dPAG by CRFr1 attenuates the anxiogenic/antinociceptive effects induced by local infusion of the NO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Corticotropin-releasing factor; Periaqueductal gray; Defensive behavior; Antinociception; Mice.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400003

High pressure near infrared study of the mutated light-harvesting complex LH2
Provedor de dados: 56
Autores: Braun,P.; Gebhardt,R.; Kwa,L.; Doster,W.
The pressure sensitivities of the near infrared spectra of the light-harvesting (LH2) complex and a mutant complex with a simplified BChl-B850 binding pocket were compared. In the mutant an abrupt change in the spectral properties occurred at 250 MPa, which was not observed with the native sample. Increased disorder due to collapse of the chromophore pocket is suggested.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Light-harvesting complex; High pressure optical spectroscopy; Protein compressibility; Antenna proteins; High pressure light scattering.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800017

Comparison of totally tubeless percutaneous nephrolithotomy and standard percutaneous nephrolithotomy for kidney stones: a randomized, clinical trial
Provedor de dados: 56
Autores: Moosanejad,N.; Firouzian,A.; Hashemi,S.A.; Bahari,M.; Fazli,M.
This study aimed to compare the totally tubeless percutaneous nephrolithotomy and standard percutaneous nephrolithotomy techniques regarding their rates of success and complications in patients with kidney stones. Patients were randomly assigned to two groups. Forty-four patients (24 men; mean age: 50.40±2.02 years) received totally tubeless percutaneous nephrolithotomy (PCNL; no nephrostomy catheter or ureteral catheter after PCNL) and 40 patients (18 men; mean age: 49.95±13.38 years) underwent standard PCNL (a nephrostomy catheter and ureteral catheter were used after PCNL). All surgeries were performed by one surgeon. Postoperative changes in hemoglobin, the blood transfusion rate, changes in creatinine levels, operation time, analgesic need,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Totally tubeless percutaneous nephrolithotomy; Standard percutaneous nephrolithotomy; Complications.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400704

Can course format influence the performance of students in an advanced cardiac life support (ACLS) program?
Provedor de dados: 56
Autores: Garrido,F.D.; Romano,M.M.D.; Schmidt,A.; Pazin-Filho,A.
Advanced cardiac life support (ACLS) is a problem-based course that employs simulation techniques to teach the standard management techniques of cardiovascular emergencies. Its structure is periodically revised according to new versions of the American Heart Association guidelines. Since it was introduced in Brazil in 1996, the ACLS has been through two conceptual and structural changes. Detailed documented reports on the effect of these changes on student performance are limited. The objective of the present study was to evaluate the effect of conceptual and structural changes of the course on student ACLS performance at a Brazilian training center. This was a retrospective study of 3266 students divided into two groups according to the teaching model:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Advanced cardiac life support; Cardiac arrest; Simulation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100004

Modulatory effects of taurine on jejunal contractility
Provedor de dados: 56
Autores: Yao,Q.Y.; Chen,D.P.; Ye,D.M.; Diao,Y.P.; Lin,Y.
Taurine (2-aminoethanesulfonic acid) is widely distributed in animal tissues and has diverse pharmacological effects. However, the role of taurine in modulating smooth muscle contractility is still controversial. We propose that taurine (5-80 mM) can exert bidirectional modulation on the contractility of isolated rat jejunal segments. Different low and high contractile states were induced in isolated jejunal segments of rats to observe the effects of taurine and the associated mechanisms. Taurine induced stimulatory effects on the contractility of isolated rat jejunal segments at 3 different low contractile states, and inhibitory effects at 3 different high contractile states. Bidirectional modulation was not observed in the presence of verapamil or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Taurine; Bidirectional modulation; Enteric nervous system; Calcium dependent; Homeostasis; Intestinal motility.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201068

Aggrecan structure in amphibian cartilage
Provedor de dados: 56
Autores: Covizi,D.Z.; Carvalho,H.F.
The structure of the large proteoglycan present in the bullfrog epiphyseal cartilage was studied by immunochemical and biochemical methods. The isolated monomer showed a polydisperse behavior on Sepharose CL2B, with a peak at Kav = 0.14. Chondroitin sulfate chains were identified by HPLC analysis of the products formed by chondroitinase digestion and mercuric acetate treatment. These chains have approximately 38 disaccharides, a Di45:Di68 ratio of 1.6 and GalNAc4S + GalNAc4,6S are the main non-reducing terminals. Keratan sulfate was identified by the use of two monoclonal antibodies in Western blots after chondroitinase ABC treatment. A keratan sulfate-rich region (~110 kDa) was isolated by sequential treatment with chondroitinase ABC and proteases. We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aggrecan; Bullfrog; Cartilage; Chondroitin sulfate; Epiphyseal cartilage.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200002

What the developing cerebral cortex tells about the adult cortex (and vice versa)
Provedor de dados: 56
Autores: Herculano-Houzel,S.; Lent,R.
Tipo: Info:eu-repo/semantics/other
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001200001

New concepts in white adipose tissue physiology
Provedor de dados: 56
Autores: Proença,A.R.G.; Sertié,R.A.L.; Oliveira,A.C.; Campaãa,A.B.; Caminhotto,R.O.; Chimin,P.; Lima,F.B.
Numerous studies address the physiology of adipose tissue (AT). The interest surrounding the physiology of AT is primarily the result of the epidemic outburst of obesity in various contemporary societies. Briefly, the two primary metabolic activities of white AT include lipogenesis and lipolysis. Throughout the last two decades, a new model of AT physiology has emerged. Although AT was considered to be primarily an abundant energy source, it is currently considered to be a prolific producer of biologically active substances, and, consequently, is now recognized as an endocrine organ. In addition to leptin, other biologically active substances secreted by AT, generally classified as cytokines, include adiponectin, interleukin-6, tumor necrosis factor-alpha,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipose tissue; Lipogenesis; Lipolysis; Adipogenesis; Endocrine.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300192

Identification of clonally rearranged T-cell receptor beta chain genes in HTLV-I carriers as a potential instrument for early detection of neoplasia
Provedor de dados: 56
Autores: Sales,M.M.; Bezerra,C.N.A.; Hiraki,Y.; Melo,N.B.; Rebouças,N.A.
We analyzed the genetic recombination pattern of the T-cell receptor beta-chain gene (TCR-beta) in order to identify clonal expansion of T-lymphocytes in 17 human T-lymphotropic virus type I (HTLV-I)-positive healthy carriers, 7 of them with abnormal features in the peripheral blood lymphocytes. Monoclonal or oligoclonal expansion of T-cells was detected in 5 of 7 HTLV-I-positive patients with abnormal lymphocytes and unconfirmed diagnosis by using PCR amplification of segments of TCR-beta gene, in a set of reactions that target 102 different variable (V) segments, covering all members of the 24 V families available in the gene bank, including the more recently identified segments of the Vbeta-5 and Vbeta-8 family and the two diversity beta segments....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human T-lymphotropic virus 1; Leukemia; Oligoclonal bands; T-cell receptor beta chain; Polymerase chain; Reaction; Adult T-cell leukemia/lymphoma.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500006

Beneficial effects of exercise training (treadmill) on insulin resistance and nonalcoholic fatty liver disease in high-fat fed C57BL/6 mice
Provedor de dados: 56
Autores: Marques,C.M.M.; Motta,V.F.; Torres,T.S.; Aguila,M.B.; Mandarim-de-Lacerda,C.A.
C57BL/6 mice develop signs and symptoms comparable, in part, to the human metabolic syndrome. The objective of the present study was to evaluate the effects of exercise training on carbohydrate metabolism, lipid profile, visceral adiposity, pancreatic islet alterations, and nonalcoholic fatty liver disease in C57BL/6 mice. Animals were fed one of two diets during an 8-week period: standard (SC, N = 12) or very high-fat (HF, N = 24) chow. An exercise training protocol (treadmill) was then established and mice were divided into SC and HF sedentary (SC-Sed, HF-Sed), exercised groups (SC-Ex, HF-Ex), or switched from HF to SC (HF/SC-Sed and HF/SC-Ex). HF/HF-Sed mice had the greatest body mass (65% more than SC/SC-Sed; P &lt; 0.0001), and exercise reduced it by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Lipid metabolism; Metabolic syndrome; Aerobic training; Fatty liver; Stereology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500008

Antagonic effect of the inhibition of inducible nitric oxide on the mortality of mice acutely infected with Escherichia coli and Bacteroides fragilis
Provedor de dados: 56
Autores: Astolfi,R.S.; Khouri,D.G.; Brandizzi,L.I.V.; Ávila-Campos,M.J.; Andrade Jr.,H.F. de.
Sepsis, the leading cause of death in intensive care units, is associated with overproduction of nitric oxide (NO) due to inducible NO synthase (iNOS), responsible for some of the pathologic changes. Aminoguanidine (AG) is a selective iNOS inhibitor with reported inconsistent actions in sepsis. To investigate the influence of iNOS, we studied models of acute bacterial sepsis using acute challenges with aerobic (Escherichia coli) and anaerobic (Bacteroides fragilis) bacteria in the presence of AG. Six-week-old, 23 g, male and female BALB/c and C57Bl/6j mice, in equal proportions, were inoculated (ip) with bacteria in groups of 4 animals for each dose and each experiment in the absence or presence of AG (50 mg/kg, ip, starting 24 h before challenge and daily...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Sepsis; Experimental model; Bacterial infections; Escherichia coli; Bacteroides fragilis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300006

IL-6 upregulation contributes to the reduction of miR-26a expression in hepatocellular carcinoma cells
Provedor de dados: 56
Autores: Zhang,Yafei; Zhang,Bicheng; Zhang,Anran; Li,Xiaohua; Liu,Jian; Zhao,Jie; Zhao,Yong; Gao,Jianfei; Fang,Dianchun; Rao,Zhiguo.
A recent study showed that miR-26a is downregulated in hepatocellular carcinoma tissues and that this downregulation is an independent predictor of survival. Interestingly, the same study also reported that miR-26a downregulation causes a concomitant elevation of IL-6 expression. Because miR-26a expression was found to be transcriptionally downregulated by oncogene c-Myc in various cancers, and the expression of c-Myc was increased by IL-6 stimulation, we hypothesized that IL-6 contributes to reduction of miR-26a in hepatocellular carcinoma. Serum IL-6 was measured by ELISA and miR-26a was detected by qRT-PCR. The data of 30 patients with hepatocellular carcinoma who had undergone surgical tumor resection revealed that serum IL-6 could be considered to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-26a; IL-6; C-Myc; Hepatocellular carcinoma; Carcinoma prognosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100032

Effect of gallium-arsenide laser, gallium-aluminum-arsenide laser and healing ointment on cutaneous wound healing in Wistar rats
Provedor de dados: 56
Autores: Gonçalves,R.V.; Mezêncio,J.M.S.; Benevides,G.P.; Matta,S.L.P.; Neves,C.A.; Sarandy,M.M.; Vilela,E.F.
This study determined the effects of gallium-aluminum-arsenide laser (GaAlAs), gallium-arsenide laser (GaAs) and Dersani® healing ointment on skin wounds in Wistar rats. The parameters analyzed were: type I and III collagen fiber concentrations as well as the rate of wound closure. Five wounds, 12 mm in diameter, were made on the animals’ backs. The depth of the surgical incision was controlled by removing the epithelial tissue until the dorsal muscular fascia was exposed. The animals were anesthetized with ketamine and xylazine via intraperitoneal injection. The rats were randomly divided into five groups of 6 animals each, according to the treatment received. Group 1 (L4): GaAs laser (4 J/cm²); group 2 (L30): GaAlAs laser (30 J/cm²); group 3 (L60):...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cicatrization; Laser; Healing ointment; Collagen I and III; Cutaneous wound.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400005

Gene expression profiling analysis of lung adenocarcinoma
Provedor de dados: 56
Autores: Xu,H.; Ma,J.; Wu,J.; Chen,L.; Sun,F.; Qu,C.; Zheng,D.; Xu,S.
The present study screened potential genes related to lung adenocarcinoma, with the aim of further understanding disease pathogenesis. The GSE2514 dataset including 20 lung adenocarcinoma and 19 adjacent normal tissue samples from 10 patients with lung adenocarcinoma aged 45-73 years was downloaded from Gene Expression Omnibus. Differentially expressed genes (DEGs) between the two groups were screened using the t-test. Potential gene functions were predicted using functional and pathway enrichment analysis, and protein-protein interaction (PPI) networks obtained from the STRING database were constructed with Cytoscape. Module analysis of PPI networks was performed through MCODE in Cytoscape. In total, 535 upregulated and 465 downregulated DEGs were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung adenocarcinoma; Pathogenesis; Differentially expressed genes; Protein-protein interaction; Network module.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300601

Association between the -174 G/C promoter polymorphism of the interleukin-6 gene and cardiovascular disease risk factors in Brazilian older women
Provedor de dados: 56
Autores: Tonet,A.C; Karnikowski,M; Moraes,C.F; Gomes,L; Karnikowski,M.G.O; Córdova,C; Nóbrega,O.T.
In worldwide studies, interleukin-6 (IL-6) is implicated in age-related disturbances. The aim of the present report was to determine the possible association of IL-6 -174 C/G promoter polymorphism with the cytokine profile as well as with the presence of selected cardiovascular risk features. This was a cross-sectional study on Brazilian women aged 60 years or older. A sample of 193 subjects was investigated for impaired glucose regulation, diabetes, hypertension, and dyslipidemia. Genotyping was done by direct sequencing of PCR products. IL-6 and C-reactive protein were quantified by high-sensitivity assays. General linear regression models or the Student t-test were used to compare continuous variables among genotypes, followed by adjustments for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokine; Interleukin-6; Genetics; Elderly; Cardiovascular disease; Brazil.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100008

Correction
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000016

Development of VEGF-loaded PLGA matrices in association with mesenchymal stem cells for tissue engineering
Provedor de dados: 56
Autores: Rosa,A.R.; Steffens,D.; Santi,B.; Quintiliano,K.; Steffen,N.; Pilger,D.A.; Pranke,P.
The association of bioactive molecules, such as vascular endothelial growth factor (VEGF), with nanofibers facilitates their controlled release, which could contribute to cellular migration and differentiation in tissue regeneration. In this research, the influence of their incorporation on a polylactic-co-glycolic acid (PLGA) scaffold produced by electrospinning on cell adhesion and viability and cytotoxicity was carried out in three groups: 1) PLGA/BSA/VEGF; 2) PLGA/BSA, and 3) PLGA. Morphology, fiber diameter, contact angle, loading efficiency and controlled release of VEGF of the biomaterials, among others, were measured. The nanofibers showed smooth surfaces without beads and with interconnected pores. PLGA/BSA/VEGF showed the smallest water contact...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Emulsion electrospinning; VEGF; Stem cells; PLGA; Biomaterials.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900601

Effects of conventional vs high-dose rocuronium on the QTc interval during anesthesia induction and intubation in patients undergoing coronary artery surgery: a randomized, double-blind, parallel trial
Provedor de dados: 56
Autores: Öztürk,T.; Ağdanlı,D.; Bayturan,Ö.; Çıkrıkcı,C.; Keleş,G.T.
Myocardial ischemia, as well as the induction agents used in anesthesia, may cause corrected QT interval (QTc) prolongation. The objective of this randomized, double-blind trial was to determine the effects of high- vs conventional-dose bolus rocuronium on QTc duration and the incidence of dysrhythmias following anesthesia induction and intubation. Fifty patients about to undergo coronary artery surgery were randomly allocated to receive conventional-dose (0.6 mg/kg, group C, n=25) or high-dose (1.2 mg/kg, group H, n=25) rocuronium after induction with etomidate and fentanyl. QTc, heart rate, and mean arterial pressure were recorded before induction (T0), after induction (T1), after rocuronium (just before laryngoscopy; T2), 2 min after intubation (T3),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: QTc; Rocuronium; Induction; Intubation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400370

Effects of sedation during upper gastrointestinal endoscopy on endocrine response and cardiorespiratory function
Provedor de dados: 56
Autores: Yetkin,G.; Oba,S.; Uludag,M.; Paksoy,I.; Akgün,I.; Eren,N.
Upper gastrointestinal endoscopy is often accompanied by tachycardia which is known to be an important pathogenic factor in the development of myocardial ischemia. The pathogenesis of tachycardia is unknown but the condition is thought to be due to the endocrine response to endoscopy. The purpose of the present study was to investigate the effects of sedation on the endocrine response and cardiorespiratory function. Forty patients scheduled for diagnostic upper gastrointestinal endoscopy were randomized into 2 groups. While the patients in the first group did not receive sedation during upper gastrointestinal endoscopy, the patients in the second group were sedated with intravenous midazolam at the dose of 5 mg for those under 65 years or 2.5 mg for those...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress response; Oxygen saturation; Sedation; Upper gastrointestinal endoscopy; Tachycardia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200008

Long-term social recognition memory in adult male rats: factor analysis of the social and non-social behaviors
Provedor de dados: 56
Autores: Moura,P.J.; Meirelles,S.T.; Xavier,G.F.
A modified version of the intruder-resident paradigm was used to investigate if social recognition memory lasts at least 24 h. One hundred and forty-six adult male Wistar rats were used. Independent groups of rats were exposed to an intruder for 0.083, 0.5, 2, 24, or 168 h and tested 24 h after the first encounter with the familiar or a different conspecific. Factor analysis was employed to identify associations between behaviors and treatments. Resident rats exhibited a 24-h social recognition memory, as indicated by a 3- to 5-fold decrease in social behaviors in the second encounter with the same conspecific compared to those observed for a different conspecific, when the duration of the first encounter was 2 h or longer. It was possible to distinguish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agonistic behavior; Long-term social recognition memory; Non-social behavior; Principal component analysis; Social behavior; Social investigation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700009

Muscle sympathetic nerve activity and hemodynamic alterations in middle-aged obese women
Provedor de dados: 56
Autores: Ribeiro,M.M.; Trombetta,I.C.; Batalha,L.T.; Rondon,M.U.P.B.; Forjaz,C.L.M.; Barretto,A.C.P.; Villares,S.M.F.; Negrão,C.E.
To study the relationship between the sympathetic nerve activity and hemodynamic alterations in obesity, we simultaneously measured muscle sympathetic nerve activity (MSNA), blood pressure, and forearm blood flow (FBF) in obese and lean individuals. Fifteen normotensive obese women (BMI = 32.5 ± 0.5 kg/m²) and 11 age-matched normotensive lean women (BMI = 22.7 ± 1.0 kg/m²) were studied. MSNA was evaluated directly from the peroneal nerve by microneurography, FBF was measured by venous occlusion plethysmography, and blood pressure was measured noninvasively by an autonomic blood pressure cuff. MSNA was significantly increased in obese women when compared with lean control women. Forearm vascular resistance and blood pressure were significantly higher in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sympathetic nerve activity; Blood pressure; Obesity.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400006

Episodes of water deprivation enhance daily hypertonic NaCl intake in rats
Provedor de dados: 56
Autores: Pereira,D.T.B.; David,R.B.; Menani,J.V.; De Luca Jr.,L.A.
Water and 1.8% NaCl intake was recorded daily in adult male rats (N = 6) submitted to four water deprivations plus four sodium appetite tests, each at the end of each 7-day interval, or in controls (non-deprived, N = 6). Water deprivation was achieved by removing water and 1.8% NaCl for 24 h. Water was then offered for 2 h. At the end of this period, 1.8% NaCl was also offered in addition to water (sodium appetite test). Average daily 1.8% NaCl intake was enhanced from 5.2 ± 1.0 to 15.7 ± 2.5 ml from the first to the fifth week in the experimental group and was unchanged in the control group. Daily water intake was not altered in either group. Thus, repeated episodes of water deprivation enhance daily NaCl intake.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Thirst; Salt appetite; Dehydration; Natriuresis.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400009

Silencing HIF-1α reduces the adhesion and secretion functions of acute leukemia hBMSCs
Provedor de dados: 56
Autores: Dong-Feng,Zeng; Ting,Liu; Cheng,Chang; Xi,Zhang; Xue,Liang; Xing-Hua,Chen; Pei-Yan,Kong.
Hypoxia inducible factor-1α (HIF-1α) is an important transcription factor, which plays a critical role in the formation of solid tumor and its microenviroment. The objective of the present study was to evaluate the expression and function of HIF-1α in human leukemia bone marrow stromal cells (BMSCs) and to identify the downstream targets of HIF-1α. HIF-1α expression was detected at both the RNA and protein levels using real-time PCR and immunohistochemistry, respectively. Vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1α (SDF-1α) were detected in stromal cells by enzyme-linked immunosorbent assay. HIF-1α was blocked by constructing the lentiviral RNAi vector system and infecting the BMSCs. The Jurkat cell/BMSC co-cultured system...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIF-1α; Human bone marrow stromal cells (hBMSCs); Leukemia; RNAi; SDF-1α; VEGF.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000004

Indole ring oxidation by activated leukocytes prevents the production of hypochlorous acid
Provedor de dados: 56
Autores: Ximenes,V.F.; Paino,I.M.M.; Faria-Oliveira,O.M.M. de; Fonseca,L.M. da; Brunetti,I.L.
Hypochlorous acid (HOCl) released by activated leukocytes has been implicated in the tissue damage that characterizes chronic inflammatory diseases. In this investigation, 14 indole derivatives, including metabolites such as melatonin, tryptophan and indole-3-acetic acid, were screened for their ability to inhibit the generation of this endogenous oxidant by stimulated leukocytes. The release of HOCl was measured by the production of taurine-chloramine when the leukocytes (2 x 10(6) cells/mL) were incubated at 37ºC in 10 mM phosphate-buffered saline, pH 7.4, for 30 min with 5 mM taurine and stimulated with 100 nM phorbol-12-myristate acetate. Irrespective of the group substituted in the indole ring, all the compounds tested including indole,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukocytes; Myeloperoxidase; Hypochlorous acid; Indole ring; Melatonin; Indole-3-acetic acid.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100003

Adverse pregnancy outcome in rats following exposure to a Salacia reticulata (Celastraceae) root extract
Provedor de dados: 56
Autores: Ratnasooriya,W.D.; Jayakody,J.R.A.C.; Premakumara,G.A.S.
The root extract of Salacia reticulata Wight (family: Celastraceae) is used in Sri Lanka by traditional practitioners as a herbal therapy for glycemic control even during pregnancy. It is recognized that some clinically used antidiabetic drugs have harmful effects on pregnancy but the effects of the S. reticulata root extract on reproductive outcome is unknown and deserves examination. We determined the effects of the S. reticulata root extract on the reproductive outcome of Wistar rats (250-260 g) when administered orally (10 g/kg) during early (days 1-7) and mid- (days 7-14) pregnancy. The root extract significantly (P&lt;0.05) enhanced post-implantation losses (control vs treatment: early pregnancy, 4.7 ± 2.4 vs 49.3 ± 13%; mid-pregnancy, 4.7 ± 2.4 vs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salacia reticulata; Hypoglycemia; Pregnancy outcome; Birth weight; Postnatal development.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700015

Isolation and culture of umbilical vein mesenchymal stem cells
Provedor de dados: 56
Autores: Covas,D.T.; Siufi,J.L.C.; Silva,A.R.L.; Orellana,M.D.
Bone marrow contains a population of stem cells that can support hematopoiesis and can differentiate into different cell lines including adipocytes, osteocytes, chondrocytes, myocytes, astrocytes, and tenocytes. These cells have been denoted mesenchymal stem cells. In the present study we isolated a cell population derived from the endothelium and subendothelium of the umbilical cord vein which possesses morphological, immunophenotypical and cell differentiation characteristics similar to those of mesenchymal stem cells isolated from bone marrow. The cells were isolated from three umbilical cords after treatment of the umbilical vein lumen with collagenase. The cell population isolated consisted of adherent cells with fibroblastoid morphology which, when...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mesenchymal stem cells; Umbilical vein; Adipocytes; Osteocytes; Cell differentiation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900006

Effect of magnesium ion on human osteoblast activity
Provedor de dados: 56
Autores: He,L.Y.; Zhang,X.M.; Liu,B.; Tian,Y.; Ma,W.H.
Magnesium, a promising biodegradable metal, has been reported in several studies to increase bone formation. Although there is some information regarding the concentrations of magnesium ions that affect bone remodeling at a cellular level, little is known about the effect of magnesium ions on cell gap junctions. Therefore, this study aimed to systematically investigate the effects of different concentrations of magnesium on bone cells, and further evaluate its effect on gap junctions of osteoblasts. Cultures of normal human osteoblasts were treated with magnesium ions at concentrations of 1, 2 and 3 mM, for 24, 48 and 72 h. The effects of magnesium ions on viability and function of normal human osteoblasts and on gap junction intercellular communication...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnesium; Osteoblast; Gap junction intercellular communication.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700604

Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis
Provedor de dados: 56
Autores: López-López,P.; Martínez-López,M.C.; Boldo-León,X.M.; Hernández-Díaz,Y.; González-Castro,T.B.; Tovilla-Zárate,C.A.; Luna-Arias,J.P.
Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dispar (non-pathogenic). The target for the PCR amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivity of the test. The study involved 62 stool samples that were collected from individuals with complaints of gastrointestinal discomfort. Of the 62 samples, 10 (16.1%) were positive for E. histolytica while 52 (83.9%) were negative. No sample was positive for E....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amebiasis; Neglected diseases; Diagnostic; PCR; DGGE; Adh112 gene.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400607

Esophageal striated muscle contractions in patients with Chagas' disease and idiopathic achalasia
Provedor de dados: 56
Autores: Dantas,R.O.; Aprile,L.R.O.; Aben-Athar,C.G.; Miranda,A.L.M.
Chagas' disease causes degeneration and reduction of the number of intrinsic neurons of the esophageal myenteric plexus, with consequent absent or partial lower esophageal sphincter relaxation and loss of peristalsis in the esophageal body. The impairment of esophageal motility is seen mainly in the distal smooth muscle region. There is no study about esophageal striated muscle contractions in the disease. In 81 patients with heartburn (44 with esophagitis) taken as controls, 51 patients with Chagas' disease (21 with esophageal dilatation) and 18 patients with idiopathic achalasia (11 with esophageal dilatation) we studied the amplitude, duration and area under the curve of esophageal proximal contractions. Using the manometric method and a continuous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esophageal contractions; Chagas' disease; Idiopathic achalasia; Esophageal striated muscle.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600007

A gasometric method to determine erythrocyte catalase activity
Provedor de dados: 56
Autores: Siqueira,A.J.S.; Remião,J.O.; Azevedo,A.M.P.; Azambuja,C.R.J.
We describe a new gasometric method to determine erythrocyte catalase activity by the measurement of the volume of oxygen produced as a result of hydrogen peroxide decomposition in a system where enzyme and substrate are separated in a special reaction test tube connected to a manometer and the reagents are mixed with a motor-driven stirrer. The position of the reagents in the test tube permits the continuous measurement of oxygen evolution from the time of mixing, without the need to stop the reaction by the addition of acid after each incubation time. The enzyme activity is reported as KHb, i.e., mg hydrogen peroxide decomposed per second per gram of hemoglobin (s-1 g Hb-1). The value obtained for catalase activity in 28 samples of hemolyzed human blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erythrocyte; Catalase assay; Gasometry.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900006

Polymorphisms in genes MTHFR, MTR and MTRR are not risk factors for cleft lip/palate in South Brazil
Provedor de dados: 56
Autores: Brandalize,A.P.C.; Bandinelli,E.; Borba,J.B.; Félix,T.M.; Roisenberg,I.; Schüler-Faccini,L.
Non-syndromic cleft lip and palate (CL/P) occurs due to interaction between genetic and environmental factors. Abnormalities in homocysteine metabolism may play a role in its etiology due to polymorphisms in genes involved in this pathway. Because of the involvement of MTHFR, MTR and MTRR genes with folate metabolism and the evidence that maternal use of folic acid in early pregnancy reduces the risk for CL/P, we evaluated the influence of their polymorphisms on the etiology of CL/P through a case-control study. The analyses involved 114 non-syndromic phenotypically white children with clefts (case) and 110 mothers, and 100 non-affected (control) children and their mothers. The polymorphisms 677C&gt;T of MTHFR, 2756A&gt;G of MTR, and 66A&gt;G of MTRR genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homocysteine; Cleft lip/palate; Methylenetetrahydrofolate reductase; Methionine synthase; Methionine synthase reductase.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600006

Genomic analysis of Brazilian patients with Fabry disease
Provedor de dados: 56
Autores: Pereira,F.S.; Jardim,L.B.; Netto,C.B.; Burin,M.G.; Cecchin,C.; Giugliani,R.; Matte,U.S.
Fabry disease is an X-linked lysosomal disorder due to a-galactosidase A deficiency that causes storage of globotriaosylceramide. The gene coding for this lysosomal enzyme is located on the long arm of the X chromosome, in region Xq21.33-Xq22. Disease progression leads to vascular disease secondary to involvement of kidney, heart and the central nervous system. Detection of female carriers based solely on enzyme assays is often inconclusive. Therefore, mutation analysis is a valuable tool for diagnosis and genetic counseling. Many mutations of the a-galactosidase A gene have been reported with high genetic heterogeneity, being most mutations private found in only one family. The disease is panethnic, and estimates of incidence range from about 1 in 40,000...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fabry disease; Lysosomal disorders; A-Galactosidase A; Globotriaosylceramide storage; GLA gene.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200002

Useful Web sites for researchers studying proteins
Provedor de dados: 56
Palavras-chave: Protein analysis; WWW; Resource.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300001

Hepatic changes in mice chronically infected with Helicobacter trogontum
Provedor de dados: 56
Autores: Moura,S.B.; Queiroz,D.M.M.; Rocha,G.A.; Comunian,L.B.; Cara,D.C.
We infected NIH germ-free female mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the lesions it induced in the liver of this host. Fifteen mice were challenged with a single dose of H. trogontum (test group) and killed 6, 12 and 18 months after inoculation (5 animals/group). Nine animals were challenged with 0.85% saline alone (control group) and killed at the same times. Fragments from the liver, cecum and colon were obtained for microbiologic and histologic examination. Stool samples were also collected. H. trogontum was detected in the cecum, colon and/or stool samples of all test mice. As expected, the bacterium was not isolated from any specimen obtained from the control animals. On the other...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Helicobacter; Helicobacter trogontum; Animal model; Liver; Mice.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900010

Drag reduction by polyethylene glycol in the tail arterial bed of normotensive and hypertensive rats
Provedor de dados: 56
Autores: Bessa,K.L.; Belletati,J.F.; Dos Santos,L.; Rossoni,L.V.; Ortiz,J.P.
This study was designed to evaluate the effect of drag reducer polymers (DRP) on arteries from normotensive (Wistar) and spontaneously hypertensive rats (SHR). Polyethylene glycol (PEG 4000 at 5000 ppm) was perfused in the tail arterial bed with (E+) and without endothelium (E-) from male, adult Wistar (N = 14) and SHR (N = 13) animals under basal conditions (constant flow at 2.5 mL/min). In these preparations, flow-pressure curves (1.5 to 10 mL/min) were constructed before and 1 h after PEG 4000 perfusion. Afterwards, the tail arterial bed was fixed and the internal diameters of the arteries were then measured by microscopy and drag reduction was assessed based on the values of wall shear stress (WSS) by computational simulation. In Wistar and SHR groups,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shear stress; Flow-pressure response; Drag reduction; Endothelium; Polyethylene glycol; Hypertension.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800006

Liver mitochondrial dysfunction and oxidative stress in the pathogenesis of experimental nonalcoholic fatty liver disease
Provedor de dados: 56
Autores: Oliveira,C.P.M.S.; Coelho,A.M.M.; Barbeiro,H.V.; Lima,V.M.R.; Soriano,F.; Ribeiro,C.; Molan,N.A.T.; Alves,V.A.F.; Souza,H.P.; Machado,M.C.C.; Carrilho,F.J.
Oxidative stress and hepatic mitochondria play a role in the pathogenesis of nonalcoholic fatty liver disease. The aim of the present study was to evaluate the role of hepatic mitochondrial dysfunction and oxidative stress in the pathogenesis of the disease. Fatty liver was induced in Wistar rats with a choline-deficient diet (CD; N = 7) or a high-fat diet enriched with PUFAs-omega-3 (H; N = 7) for 4 weeks. The control group (N = 7) was fed a standard diet. Liver mitochondrial oxidation and phosphorylation were measured polarographically and oxidative stress was estimated on the basis of malondialdehyde and glutathione concentrations. Moderate macrovacuolar liver steatosis was observed in the CD group and mild liver steatosis was observed in the periportal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic mitochondrial dysfunction; Oxidative stress; Nonalcoholic fatty liver disease; Choline-deficient diet; High-fat diet.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200004

Hepatitis C virus seroprevalence and genotypes in patients with diffuse connective tissue diseases and spondyloarthropathies
Provedor de dados: 56
Autores: Barbosa,V.S.; Silva,N.A. da; Martins,R.M.B.
Many extrahepatic manifestations, including rheumatic diseases, have been reported to be associated with hepatitis C virus (HCV) infection. In order to investigate the prevalence of HCV infection among patients with rheumatic diseases, in the present study we interviewed 367 patients and tested their blood samples for HCV antibodies (anti-HCV) by an enzyme-linked immunosorbent assay. Anti-HCV-reactive samples were retested for confirmation by a line immunoassay and also for HCV RNA detection by the polymerase chain reaction. HCV RNA-positive samples were genotyped by INNO-LIPA. An overall HCV infection prevalence of 1.9% (7/367) was found. Of the 7 HCV-infected patients, 4 had systemic lupus erythematosus and 3 rheumatoid arthritis, resulting in positivity...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis C; Rheumatic disease; Arthritis; INNO-LIPA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500020

Association between elevated adiponectin level and adverse outcomes in patients with heart failure: a systematic review and meta-analysis
Provedor de dados: 56
Autores: Bai,Wenwei; Huang,Jingjing; Zhu,Min; Liu,Xiaoyong; Tao,Jianping.
Studies regarding the prognostic value of circulating adiponectin level in patients with heart failure are conflicting. The aim of this meta-analysis was to evaluate the association between elevated circulating adiponectin level and adverse outcomes in patients with heart failure. We searched PubMed and Embase databases from their inception to June 2018. Original observational studies that investigated the prognostic value of adiponectin in heart failure patients and reported all-cause mortality or combined endpoints of death/readmission as outcome measure were included. Pooled risk ratio (RR) with 95% confidence intervals (CI) were estimated by higher versus lower circulating adiponectin level. A total of 7 studies involving 862 heart failure patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adiponectin; Heart failure; Mortality; Readmission; Meta-analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700610

Correlation between symptoms of the irritable bowel syndrome and the response to the food extract skin prick test
Provedor de dados: 56
Autores: Soares,R.L.S.; Figueiredo,H.N.; Maneschy,C.P.; Rocha,V.R.S.; Santos,J.M.
The relationship between the irritable bowel syndrome (IBS) and food intolerance is not clear. We studied the cutaneous response to food antigens in 43 volunteers who were students and employees of the Faculty of Medicine of Universidade Federal Fluminense. Subjects were divided into 3 groups after evaluation for Roma II criteria for functional disease of the gastrointestinal tract: group I, 14 volunteers with IBS; group II, 15 volunteers with functional dyspepsia; group III, 14 volunteers without habitual gastrointestinal symptoms. The subjects were submitted to the skin prick test with 9 food antigen extracts, for a total of 387 skin tests (9 per volunteer). Of the 126 tests applied to group I, 24 (19.4%) were positive (a 3-mm wider papule than the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Irritable bowel syndrome; Food intolerance; Skin prick test.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500005

Exercise training prevents increased intraocular pressure and sympathetic vascular modulation in an experimental model of metabolic syndrome
Provedor de dados: 56
Autores: Castro,E.F.S.; Mostarda,C.T.; Rodrigues,B.; Moraes-Silva,I.C.; Feriani,D.J.; De Angelis,K.; Irigoyen,M.C.
The present study aimed to study the effects of exercise training (ET) performed by rats on a 10-week high-fructose diet on metabolic, hemodynamic, and autonomic changes, as well as intraocular pressure (IOP). Male Wistar rats receiving fructose overload in drinking water (100 g/L) were concomitantly trained on a treadmill for 10 weeks (FT group) or kept sedentary (F group), and a control group (C) was kept in normal laboratory conditions. The metabolic evaluation comprised the Lee index, glycemia, and insulin tolerance test (KITT). Arterial pressure (AP) was measured directly, and systolic AP variability was performed to determine peripheral autonomic modulation. ET attenuated impaired metabolic parameters, AP, IOP, and ocular perfusion pressure (OPP)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fructose; Exercise training; Intraocular pressure; Autonomic modulation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400332

Pharmacokinetic and parasitological evaluation of the bone marrow of dogs with visceral leishmaniasis submitted to multiple dose treatment with liposome-encapsulated meglumine antimoniate
Provedor de dados: 56
Autores: Schettini,D.A.; Costa Val,A.P.; Souza,L.F.; Demicheli,C.; Rocha,O.G.F.; Melo,M.N.; Michalick,M.S.M.; Frézard,F.
The aim of the present study was to evaluate the impact of a multiple dose regimen of a liposomal formulation of meglumine antimoniate (LMA) on the pharmacokinetics of antimony in the bone marrow of dogs with visceral leishmaniasis and on the ability of LMA to eliminate parasites from this tissue. Dogs naturally infected with Leishmania chagasi received 4 intravenous doses of either LMA (6.5 mg antimony/kg body weight, N = 9), or empty liposomes (at the same lipid dose as LMA, N = 9) at 4-day intervals. A third group of animals was untreated (N = 8). Before each administration and at different times after treatment, bone marrow was obtained and analyzed for antimony level (LMA group) by electrothermal atomic absorption spectrometry, and for the presence of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Liposome; Meglumine antimoniate; Pharmacokinetics; Leishmaniasis; Dogs; Bone marrow.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200017

Frequency of Werner helicase 1367 polymorphism and age-related morbidity in an elderly Brazilian population
Provedor de dados: 56
Autores: Smith,M.A.C.; Silva,M.D.A.; Araujo,L.Q.; Ramos,L.R.; Labio,R.W.; Burbano,R.R.; Peres,C.A.; Andreoli,S.B.; Payão,S.L.M.; Cendoroglo,M.S.
Werner syndrome (WS) is a premature aging disease caused by a mutation in the WRN gene. The gene was identified in 1996 and its product acts as a DNA helicase and exonuclease. Some specific WRN polymorphic variants were associated with increased risk for cardiovascular diseases. The identification of genetic polymorphisms as risk factors for complex diseases affecting older people can improve their prevention, diagnosis and prognosis. We investigated WRN codon 1367 polymorphism in 383 residents in a district of the city of São Paulo, who were enrolled in an Elderly Brazilian Longitudinal Study. Their mean age was 79.70 ± 5.32 years, ranging from 67 to 97. This population was composed of 262 females (68.4%) and 121 males (31.6%) of European (89.2%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: WRN: codon 1367 polymorphism; Age-related morbidities; Elderly cohort study.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700008

Relative performance of the two hands in simple and choice reaction time tasks
Provedor de dados: 56
Autores: Nisiyama,M.; Ribeiro-do-Valle,L.E.
There is evidence that the left hemisphere is more competent for motor control than the right hemisphere. This study investigated whether this hemispheric asymmetry is expressed in the latency/duration of sequential responses performed by the left and/or right hands. Thirty-two right-handed young adults (16 males, 16 females; 18-25 years old) were tested in a simple or choice reaction time task. They responded to a left and/or right visual target by moving their left and/or right middle fingers between two keys on each side of the midline. Right hand reaction time did not differ from left hand reaction time. Submovement times were longer for the right hand than the left hand when the response was bilateral. Pause times were shorter for the right hand than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Motor lateral asymmetry; Reaction time; Movement time; Response complexity; Unilateral response; Bilateral response.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100080

Biochemical profile of amniotic fluid for the assessment of fetal and renal development
Provedor de dados: 56
Autores: Oliveira,F.R.; Barros,E.G.; Magalhães,J.A.
Creatinine plays a key role in the function and maturation of fetal kidneys throughout pregnancy. It is important to identify other markers that may help in the diagnosis of renal dysfunction. Our aim was to determine the profile of and the correlation between biochemical markers to be used to assess renal function and maturation of the fetus in the amniotic fluid during pregnancy and to determine the distribution of normal values for creatinine, N-acetyl-ß-D-glucosaminidase (NAG), ß2-microglobulin, glucose, urea, sodium, potassium, phosphorus, calcium, uric acid, albumin, and osmolality in three gestational age groups. This was a cross-section study that assessed 115 samples of amniotic fluid during three different periods of pregnancy, i.e., 13 to 20, 27...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fetal renal maturation; Normal pregnancy; Amniotic fluid; Creatinine; SS2-Microglobulin; Glucose; Uric acid.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200010

Effect of silybin on high-fat-induced fatty liver in rats
Provedor de dados: 56
Autores: Yao,Jiayin; Zhi,Min; Minhu,Chen.
Silybin, a natural antioxidant, has been traditionally used against a variety of liver ailments. To investigate its effect and the underlying mechanisms of action on non-alcoholic fatty liver in rats, we used 60 4-6-week-old male Sprague-Dawley rats to establish fatty liver models by feeding a high-fat diet for 6 weeks. Hepatic enzyme, serum lipid levels, oxidative production, mitochondrial membrane fluidity, homeostasis model assessment-insulin resistance index (HOMA-IR), gene and protein expression of adiponectin, and resistin were evaluated by biochemical, reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. Compared with the model group, silybin treatment (26.25 mg·kg-1·day-1, started at the beginning of the protocol)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-alcoholic fatty liver disease; Mitochondrial membrane fluidity; Lipid peroxidation; Insulin resistance; Silybin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700007

Plasma lipid abnormalities in Pakistani population: trends, associated factors, and clinical implications
Provedor de dados: 56
Autores: Zaid,M.; Hasnain,S.
Previous studies have reported increased prevalence of coronary heart disease (CHD) in Indians and South Asian settlers in North America. This increased burden of CHD among South Asians is mainly caused by dyslipidemia. To the best of our knowledge, none of the previous works has studied the patterns and prevalence of dyslipidemia in the Pakistani population. The present work aimed to study the plasma lipid trends and abnormalities in a population-based sample of urban and rural Pakistanis. The study included 238 participants (108 males,130 females). Plasma lipid profiles of the participants were determined using standard protocols. We observed that 63% of the study population displayed irregularities in at least one major lipid-fraction including total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plasma Lipids; Dyslipidemia; Coronary heart disease.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900609

Structural, functional and immunological studies on a polymeric bacterial protein
Provedor de dados: 56
Autores: Baldi,P.C.; Velikovsky,C.A.; Braden,B.C.; Giambartolomei,G.H.; Fossati,C.A.; Goldbaum,F.A.
The characterization of proteins from Brucella spp, the causative agent of brucellosis, has been the subject of intensive research. We have described an 18-kDa cytoplasmic protein of Brucella abortus and shown the potential usefulness of this protein as an antigen for the serologic diagnosis of brucellosis. The amino acid sequence of the protein showed a low but significant homology with that of lumazine synthases. Lumazine is an intermediate product in bacterial riboflavin biosynthesis. The recombinant form of the 18-kDa protein (expressed in E. coli) folds like the native Brucella protein and has lumazine-synthase enzymatic activity. Three-dimensional analysis by X-ray crystallography of the homolog Bacillus subtilis lumazine synthase has revealed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucella lumazine synthase; X-ray structure; Immunogenicity; Brucellosis detection; Bacillus subtilis lumazine synthase.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700003

Reaction of diphenyl diselenide with hydrogen peroxide and inhibition of delta-aminolevulinate dehydratase from rat liver and cucumber leaves
Provedor de dados: 56
Autores: Farina,M.; Barbosa,N.B.V.; Nogueira,C.W.; Folmer,V.; Zeni,G.; Andrade,L.H.; Braga,A. L.; Rocha,J.B.T.
The interaction of the product of H2O2 and (PhSe)2 with delta-aminolevulinate dehydratase (delta-ALA-D) from mammals and plants was investigated. (PhSe)2 inhibited rat hepatic delta-ALA-D with an IC50 of 10 µM but not the enzyme from cucumber leaves. The reaction of (PhSe)2 with H2O2 for 1 h increased the inhibitory potency of the original compound and the IC50 for animal delta-ALA-D inhibition was decreased from 10 to 2 µM. delta-ALA-D from cucumber leaves was also inhibited by the products of reaction of (PhSe)2 with H2O2 with an IC50 of 4 µM. The major product of reaction of (PhSe)2 with H2O2 was identified as seleninic acid and produced an intermediate with a lambdamax at 265 nm after reaction with t-BuSH. These results suggest that the interaction of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Delta-Aminolevulinate dehydratase; Diphenyl diselenide; Seleninic acid.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600001

Adrenal incidentaloma
Provedor de dados: 56
Autores: Arnaldi,G.; Masini,A.M.; Giacchetti,G.; Taccaliti,A.; Faloia,E.; Mantero,F.
Incidentally discovered adrenal masses, or adrenal incidentalomas, have become a common clinical problem owing to wide application of radiologic imaging techniques. This definition encompasses a heterogeneous spectrum of pathologic entities, including primary adrenocortical and medullary tumors, benign or malignant lesions, hormonally active or inactive lesions, metastases, and infections. Once an adrenal mass is detected, the clinician needs to address two crucial questions: is the mass malignant, and is it hormonally active? This article provides an overview of the diagnostic clinical approach and management of the adrenal incidentaloma. Mass size is the most reliable variable to distinguish benign and malignant adrenal masses. Adrenalectomy should be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenal incidentaloma; Subclinical Cushing's syndrome; Silent pheochromocytoma; Aldosterone-producing adenoma.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000007

Relationship between microalbuminuria and cardiac structural changes in mild hypertensive patients
Provedor de dados: 56
Autores: Plavnik,F.L.; Silva,M.A.M.R.T.; Kohlmann,N.E.B.; Kohlmann Jr.,O.; Ribeiro,A.B.; Zanella,M.T.
The aim of this study was to determine the relationship between urinary albumin excretion (UAE), cardiac structural changes upon echocardiography and 24-h ambulatory blood pressure (ABPM) levels. Twenty mild hypertensive patients (mean age 56.8 ± 9.6 years) were evaluated. After 2 weeks of a washout period of all antihypertensive drugs, all patients underwent an echocardiographic evaluation, a 24-h ABPM and an overnight urine collection. Systolic and diastolic blood pressure during 24-h ABPM was 145 ± 14/91 ± 10 mmHg (daytime) and 130 ± 14/76 ± 8 mmHg (nighttime), respectively. Seven (35%) patients presented UAE  &gt; or = 15 µg/min, and for the whole group, the geometric mean value for UAE was 10.2 x/÷ 3.86 µg/min. Cardiac measurements showed mean values...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Microalbuminuria; Left ventricular hypertrophy; Blood pressure monitoring.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700006

The role of Ca2+/calmodulin-dependent protein kinase II and calcineurin in TNF-α-induced myocardial hypertrophy
Provedor de dados: 56
Autores: Wang,Gui-Jun; Wang,Hong-Xin; Yao,Yu-Sheng; Guo,Lian-Yi; Liu,Pei.
We investigated whether Ca2+/calmodulin-dependent kinase II (CaMKII) and calcineurin (CaN) are involved in myocardial hypertrophy induced by tumor necrosis factor α (TNF-α). The cardiomyocytes of neonatal Wistar rats (1-2 days old) were cultured and stimulated by TNF-α (100 μg/L), and Ca2+ signal transduction was blocked by several antagonists, including BAPTA (4 µM), KN-93 (0.2 µM) and cyclosporin A (CsA, 0.2 µM). Protein content, protein synthesis, cardiomyocyte volumes, [Ca2+]i transients, CaMKIIδB and CaN were evaluated by the Lowry method, [³H]-leucine incorporation, a computerized image analysis system, a Till imaging system, and Western blot analysis, respectively. TNF-α induced a significant increase in protein content in a dose-dependent manner...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial hypertrophy; Tumor necrosis factor α; Calcium; Calmodulin-dependent kinase; Calcineurin.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100008

In vitro larvicidal activity of geraniol and citronellal against Contracaecum sp (Nematoda: Anisakidae)
Provedor de dados: 56
Autores: Barros,L.A.; Yamanaka,A.R.; Silva,L.E.; Vanzeler,M.L.A.; Braum,D.T.; Bonaldo,J.
Human infection with fish parasites can result from the ingestion of incompletely cooked or raw fish, giving origin to parasitic diseases such as anisakiasis, caused by parasites of the Anisakidae family. The present study assessed the in vitro larvicidal effect of two monoterpene compounds, geraniol and citronellal, against Contracaecum sp (Nematoda: Anisakidae). Four hundred live larvae of Contracaecum sp obtained from "traíra" fish (Hoplias malabaricus, Bloch, 1974) were analyzed on 40 Petri dishes (10 larvae each) with the compounds to be tested. The final concentrations tested for each compound were 250, 125, 62.5, and 31.2 µg/mL and the evaluation was carried out at five different times (2, 4, 8, 24, and 48 h). The larvicidal action of geraniol and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Geraniol; Citronellal; Larvicide; Contracaecum sp.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000007

Effect of inhibition of nitric oxide synthase on blood pressure and renal sodium handling in renal denervated rats
Provedor de dados: 56
Autores: Xavier,F.; Magalhães,A.M.F.; Gontijo,J.A.R.
The role of sympathetic nerve activity in the changes in arterial blood pressure and renal function caused by the chronic administration of NG-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) synthesis, was examined in sham and bilaterally renal denervated rats. Several studies have demonstrated that sympathetic nerve activity is elevated acutely after L-NAME administration. To evaluate the role of renal nerve activity in L-NAME-induced hypertension, we compared the blood pressure response in four groups (N = 10 each) of male Wistar-Hannover rats weighing 200 to 250 g: 1) sham-operated vehicle-treated, 2) sham-operated L-NAME-treated, 3) denervated vehicle-treated, and 4) denervated L-NAME-treated rats. After renal denervation or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Hypertension; Natriuresis; Renal denervation; Lithium clearance.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300014

Reflex control of arterial pressure and heart rate in short-term streptozotocin diabetic rats
Provedor de dados: 56
Autores: Dall'Ago,P.; Silva,V.O.K.; De Angelis,K.L.D.; Irigoyen,M.C.; Fazan Jr.,R.; Salgado,H.C.
Impaired baroreflex sensitivity in diabetes is well described and has been attributed to autonomic diabetic neuropathy. In the present study conducted on acute (10-20 days) streptozotocin (STZ)-induced diabetic rats we examined: 1) cardiac baroreflex sensitivity, assessed by the slope of the linear regression between phenylephrine- or sodium nitroprusside-induced changes in arterial pressure and reflex changes in heart rate (HR) in conscious rats; 2) aortic baroreceptor function by means of the relationship between systolic arterial pressure and aortic depressor nerve (ADN) activity, in anesthetized rats, and 3) bradycardia produced by electrical stimulation of the vagus nerve or by the iv injection of methacholine in anesthetized animals. Reflex...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baroreflex; Baroreceptors; Streptozotocin; Vagus nerve.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700013

Effects of different levels of positive airway pressure on breathing pattern and heart rate variability after coronary artery bypass grafting surgery
Provedor de dados: 56
Autores: Pantoni,C.B.F.; Di Thommazo,L.; Mendes,R.G.; Catai,A.M.; Luzzi,S.; Amaral Neto,O.; Borghi-Silva,A.
The application of continuous positive airway pressure (CPAP) produces important hemodynamic alterations, which can influence breathing pattern (BP) and heart rate variability (HRV). The aim of this study was to evaluate the effects of different levels of CPAP on postoperative BP and HRV after coronary artery bypass grafting (CABG) surgery and the impact of CABG surgery on these variables. Eighteen patients undergoing CABG were evaluated postoperatively during spontaneous breathing (SB) and application of four levels of CPAP applied in random order: sham (3 cmH2O), 5 cmH2O, 8 cmH2O, and 12 cmH2O. HRV was analyzed in time and frequency domains and by nonlinear methods and BP was analyzed in different variables (breathing frequency, inspiratory tidal volume,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Continuous positive airway pressure; Cardiovascular system; Autonomic nervous system; Ventilation; Coronary artery bypass.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100006

PPM1B and P-IKKβ expression levels correlated inversely with rat gastrocnemius atrophy after denervation
Provedor de dados: 56
Autores: Wei,Jian; Liang,Bing-Sheng.
Activated inhibitor of nuclear factor-κB kinase β (IKKβ) is necessary and sufficient for denervated skeletal muscle atrophy. Although several studies have shown that Mg2+/Mn2+-dependent protein phosphatase 1B (PPM1B) inactivated IKKβ, few studies have investigated the role of PPM1B in denervated skeletal muscle. In this study, we aim to explore the expression and significance of PPM1B and phosphorylated IKKβ (P-IKKβ) during atrophy of the denervated gastrocnemius. Thirty young adult female Wistar rats were subjected to right sciatic nerve transection and were sacrificed at 0 (control), 2, 7, 14, and 28 days after denervation surgery. The gastrocnemius was removed from both the denervated and the contralateral limb. The muscle wet weight ratio was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscular atrophy; Denervation; PPM1B; P-IKKβ; IKKβ; RT-PCR; Western blot.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800004

Prevalence of serological markers of hepatitis B virus in pregnant women from Paraná State, Brazil
Provedor de dados: 56
Autores: Bertolini,D.A.; Pinho,J.R.R.; Saraceni,C.P.; Moreira,R.C.; Granato,C.F.H.; Carrilho,F.J.
The prevalence of hepatitis B virus (HBV) in Brazil increases from South to North but moderate to elevated prevalence has been detected in the Southwest of Paraná State. The prevalence of serological markers of HBV was evaluated in 3188 pregnant women from different counties in Paraná State and relevant epidemiological features were described. The prevalence of HBV markers in pregnant women for the state as a whole was 18.5% (95% CI = 17.2-19.9), ranging from 7.2% in Curitiba to 38.5% in Francisco Beltrão. The endemicity of HBV marker prevalence in pregnant women was intermediate in Cascavel, Foz do Iguaçu, and Francisco Beltrão, and low in Curitiba, Londrina, Maringá, and Paranaguá. Multiple logistic regression showed that HBV marker prevalence increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B virus; Brazil; Serological markers; Pregnant women.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800011

Spectroscopic studies on the interaction of efonidipine with bovine serum albumin
Provedor de dados: 56
Autores: Wang,N.; Ye,L.; Zhao,B.Q.; Yu,J.X.
Efonidipine hydrochloride is an antihypertensive and antianginal agent with fewer side effects and is better tolerated in the treatment of hypertension with renal impairment. Its interaction with bovine serum albumin (BSA) is of great use for the understanding of the pharmacokinetic and pharmacodynamic mechanisms of the drug. The binding of efonidipine to BSA was investigated by fluorescence spectroscopy and circular dichroism. BSA fluorescence was quenched by efonidipine, due to the fact that efonidipine quenched the fluorescence of tryptophan residues mainly by the collision mode. The thermodynamic parameters ΔH0 and ΔS0 were 68.04 kJ/mol and 319.42 J·mol-1·K-1, respectively, indicating that the hydrophobic interactions played a major role. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Efonidipine; Bovine serum albumin; Fluorescence quenching; Synchronous fluorescence; Circular dichroism.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700007

Changes in the electrophysiological parameters of the posterior intestine of Anguilla anguilla (Pisces) induced by oxytocin, urotensin II and aldosterone
Provedor de dados: 56
In view of the importance of the intestine in the osmoregulation of freshwater fishes, we determined the effects of oxytocin, urotensin II (UII), and aldosterone added to the serosal side of the isolated posterior intestine of the freshwater-adapted teleost Anguilla anguilla on electrophysiological parameters. Oxytocin decreased the short-circuit current (SCC) and transepithelial potential difference (TPD) at concentrations of 1 and 10 mU/ml (to 50% and 42% of control values, respectively), but did not alter these parameters at a concentration of 0.1 mU/ml. UII reduced SCC and TPD at concentrations of 10 nM, 50 nM and 100 nM (to 85% of control values), but increased these parameters at the concentration of 500 nM (to 115% of control values). Aldosterone...
Palavras-chave: Intestine; Anguilla anguilla; Oxytocin; Urotensin II; Aldosterone.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100006

Genotyping hepatitis C virus from hemodialysis patients in Central Brazil by line probe assay and sequence analysis
Provedor de dados: 56
Autores: Espírito-Santo,M.P.; Carneiro,M.A.S.; Reis,N.R.S.; Kozlowski,A.G.; Teles,S.A.; Lampe,E.; Yoshida,C.F.T.; Martins,R.M.B.
The present study examined the distribution of hepatitis C virus (HCV) genotypes and subtypes in a hemodialysis population in Goiás State, Central Brazil, and evaluated the efficiency of two genotyping methods: line probe assay (LiPA) based on the 5' noncoding region and nucleotide sequencing of the nonstructural 5B (NS5B) region of the genome. A total of 1095 sera were tested for HCV RNA by RT-nested PCR of the 5' noncoding region. The LiPA assay was able to genotype all 131 HCV RNA-positive samples. Genotypes 1 (92.4%) and 3 (7.6%) were found. Subtype 1a (65.7%) was the most prevalent, followed by subtypes 1b (26.7%) and 3a (7.6%). Direct nucleotide sequencing of 340 bp from the NS5B region was performed in 106 samples. The phylogenetic tree showed that...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis C; Hepatitis C virus; Genotypes; Hemodialysis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400013

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes
Provedor de dados: 56
Autores: Costa,L.O.B.; Mendes,M.C.; Ferriani,R.A.; Moura,M.D.; Reis,R.M.; Silva de Sá,M.F.
The objective of the present study was to examine the association between follicular fluid (FF) steroid concentration and oocyte maturity and fertilization rates. Seventeen infertile patients were submitted to ovulation induction with urinary human follicle-stimulating hormone, human menopausal gonadotropin and human chorionic gonadotropin (hCG). A total of 107 follicles were aspirated after hCG administration, the oocytes were analyzed for maturity and 81 of them were incubated and inseminated in vitro. Progesterone, estradiol (E2), estrone, androstenedione, and testosterone were measured in the FF. E2 and testosterone levels were significantly higher in FF containing immature oocytes (median = 618.2 and 16 ng/ml, respectively) than in FF containing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Steroid in follicular fluid; Maturity of oocytes; In vitro fertilization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100021

Effect of endogenous hydrogen sulfide inhibition on structural and functional renal disturbances induced by gentamicin
Provedor de dados: 56
Autores: Francescato,H.D.C.; Chierice,J.R.A.; Marin,E.C.S.; Cunha,F.Q.; Costa,R.S.; Silva,C.G.A.; Coimbra,T.M.
Animal models of gentamicin nephrotoxicity present acute tubular necrosis associated with inflammation, which can contribute to intensify the renal damage. Hydrogen sulfide (H2S) is a signaling molecule involved in inflammation. We evaluated the effect of DL-propargylglycine (PAG), an inhibitor of endogenous H2S formation, on the renal damage induced by gentamicin. Male Wistar rats (N = 8) were injected with 40 mg/kg gentamicin (im) twice a day for 9 days, some of them also received PAG (N = 8, 10 mg·kg-1·day-1, ip). Control rats (N = 6) were treated with saline or PAG only (N = 4). Twenty-four-hour urine samples were collected one day after the end of these treatments, blood samples were collected, the animals were sacrificed, and the kidneys were removed...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Inflammation; Gentamicin nephrotoxicity; DL-propargylglycine; Hydrogen sulfide; Acute tubular necrosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300010

Thermoregulation in hypertensive men exercising in the heat with water ingestion
Provedor de dados: 56
Autores: Ribeiro,G.A.; Rodrigues,L.O.C.; Moreira,M.C.V.; Silami-Garcia,E.; Pascoa,M.R.S.; Camargos,F.F.O.
Hydration is recommended in order to decrease the overload on the cardiovascular system when healthy individuals exercise, mainly in the heat. To date, no criteria have been established for hydration for hypertensive (HY) individuals during exercise in a hot environment. Eight male HY volunteers without another medical problem and 8 normal (NO) subjects (46 ± 3 and 48 ± 1 years; 78.8 ± 2.5 and 79.5 ± 2.8 kg; 171 ± 2 and 167 ± 1 cm; body mass index = 26.8 ± 0.7 and 28.5 ± 0.6 kg/m²; resting systolic (SBP) = 142.5 and 112.5 mmHg and diastolic blood pressure (DBP) = 97.5 and 78.1 mmHg, respectively) exercised for 60 min on a cycle ergometer (40% of VO2peak) with (500 ml 2 h before and 115 ml every 15 min throughout exercise) or without water ingestion, in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Exercise; Water ingestion; Heat; Thermoregulation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300019

Role of Th1 and Th2 cells in autoimmune demyelinating disease
Provedor de dados: 56
Autores: Nagelkerken,L.
Evidence is accumulating that Th1 cells play an important role in the development of multiple sclerosis (MS) and experimental allergic encephalomyelitis (EAE), whereas Th2 cells contribute to recovery from disease. A major determinant in the development of Th1 and Th2 cells is the type of antigen-presenting cell (APC) involved and its functional characteristics, e.g., the production of interleukin-12. Therefore, modulation of APC might interfere with the development of Th1 type responses and as such be beneficial for MS and EAE. The potential of cytokines, in particular interleukin-10, and glucocorticoids to exert a selective effect on APC, and as a consequence to affect the Th1-Th2 balance in EAE, is discussed
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiple sclerosis; Experimental allergic encephalomyelitis; Cytokines; Glucocorticoids; Th1 cells; Antigen-presenting cells.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100007

Inhibition of gastric emptying and intestinal transit in anesthetized rats by a Tityus serrulatus scorpion toxin
Provedor de dados: 56
Autores: Troncon,L.E.A.; Santos,A.A.; Garbacio,V.L.; Secaf,M.; Verceze,A.V.; Cunha-Melo,J.R.
The effects of a fraction (T1) of Tityus serrulatus scorpion venom prepared by gel filtration on gastric emptying and small intestinal transit were investigated in male Wistar rats. Fasted animals were anesthetized with urethane, submitted to tracheal intubation and right jugular vein cannulation. Scorpion toxin (250 µg/kg) or saline was injected iv and 1 h later a bolus of saline (1.0 ml/100 g) labeled with 99m technetium-phytate (10 MBq) was administered by gavage. After 15 min, animals were sacrificed and the radioactivity remaining in the stomach was determined. Intestinal transit was evaluated by instillation of a technetium-labeled saline bolus (1.0 ml) through a cannula previously implanted in the duodenum. After 60 min, the progression of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion toxin; Gastric emptying; Intestinal transit; Gastrointestinal motility.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900010

Hormonal therapy with estradiol and drospirenone improves endothelium-dependent vasodilation in the coronary bed of ovariectomized spontaneously hypertensive rats
Provedor de dados: 56
Autores: Borgo,M.V.; Claudio,E.R.G.; Silva,F.B.; Romero,W.G.; Gouvea,S.A.; Moysés,M.R.; Santos,R.L.; Almeida,S.A.; Podratz,P.L.; Graceli,J.B.; Abreu,G.R.
Drospirenone (DRSP) is a progestin with anti-aldosterone properties and it reduces blood pressure in hypertensive women. However, the effects of DRSP on endothelium-dependent coronary vasodilation have not been evaluated. This study investigated the effects of combined therapy with estrogen (E2) and DRSP on endothelium-dependent vasodilation of the coronary bed of ovariectomized (OVX) spontaneously hypertensive rats. Female spontaneously hypertensive rats (n=87) at 12 weeks of age were randomly divided into sham operated (Sham), OVX, OVX treated with E2 (E2), and OVX treated with E2 and DRSP (E2+DRSP) groups. Hemodynamic parameters were directly evaluated by catheter insertion into the femoral artery. Endothelium-dependent vasodilation in response to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Menopause; Hypertension; Hormone therapy; Drospirenone; Coronary reactivity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100601

Myocyte necrosis is the basis for fibrosis in  renovascular hypertensive rats
Provedor de dados: 56
Autores: Okoshi,M.P.; Matsubara,L.S.; Franco,M.; Cicogna,A.C.; Matsubara,B.B.
The pathogenesis of fibrosis and the functional features of pressure overload myocardial hypertrophy are still controversial. The objectives of the present study were to evaluate the function and morphology of the hypertrophied myocardium in renovascular hypertensive (RHT) rats. Male Wistar rats were sacrificed at week 4 (RHT4) and 8 (RHT8) after unilateral renal ischemia (Goldblatt II hypertension model). Normotensive rats were used as controls. Myocardial function was analyzed in isolated papillary muscle preparations, morphological features were defined by light microscopy, and myocardial hydroxyproline concentration (HOP) was determined by spectrophotometry. Renal artery clipping resulted in elevated systolic arterial pressure (RHT4: 178 ± 19 mmHg and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary vascular remodeling; Myocardial function; Pressure overload hypertrophy; Papillary muscle; Reparative fib.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900013

Application of endoscopy to treat mandibular keratocystic odontogenic tumors
Provedor de dados: 56
Autores: Gao,Z.; Ni,Q.W.; Gao,W.; Liu,Y.P.; Zhang,Q.
The aim of this study was to evaluate the feasibility of endoscopy to remove keratocystic odontogenic tumors (KCOTs) with virtual 3D mandibular images. Fifteen patients (mean age, 40.27±14.58 years) who underwent endoscopic mandibular KCOT enucleation between May 2009 and October 2009 were included. Virtual 3D mandibular reconstructions derived from computed tomography (CT) imaging were generated for all patients. Recurrence and pathological fracture were evaluated as the primary outcome variables at 1 and 12 months after operation. Secondary infection and inferior alveolar nerve injury were evaluated as the secondary outcome variables at 1 and 6 months after operation. None of the 15 patients exhibited signs of recurrence or pathological fracture after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mandibular KCOT; Endoscopic assisted surgery; 3D reconstruction; Minimally invasive surgical procedures; Treatment feasibility.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800605

Longitudinal brain volumetric changes during one year in non-elderly healthy adults: a voxel-based morphometry study
Provedor de dados: 56
Autores: Guimarães,R.M.; Schaufelberger,M.S.; Santos,L.C.; Duran,F.L.S.; Menezes,P.R.; Scazufca,M.; Gouvea,M.T.V; Busatto,G.F.
Previous cross-sectional magnetic resonance imaging (MRI) studies of healthy aging in young adults have indicated the presence of significant inverse correlations between age and gray matter volumes, although not homogeneously across all brain regions. However, such cross-sectional studies have important limitations and there is a scarcity of detailed longitudinal MRI studies with repeated measures obtained in the same individuals in order to investigate regional gray matter changes during short periods of time in non-elderly healthy adults. In the present study, 52 healthy young adults aged 18 to 50 years (27 males and 25 females) were followed with repeated MRI acquisitions over approximately 15 months. Gray matter volumes were compared between the two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gray matter; Aging; Voxel-based morphometry; Magnetic resonance imaging; Prefrontal cortex; Hippocampus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600007

Differential binding with ERα and ERβ of the phytoestrogen-rich plant Pueraria mirifica
Provedor de dados: 56
Autores: Boonchird,C.; Mahapanichkul,T.; Cherdshewasart,W.
Variations in the estrogenic activity of the phytoestrogen-rich plant, Pueraria mirifica, were determined with yeast estrogen screen (YES) consisting of human estrogen receptors (hER) hERα and hERβ and human transcriptional intermediary factor 2 (hTIF2) or human steroid receptor coactivator 1 (hSRC1), respectively, together with the β-galactosidase expression cassette. Relative estrogenic potency was expressed by determining the β-galactosidase activity (EC50) of the tuber extracts in relation to 17β-estradiol. Twenty-four and 22 of the plant tuber ethanolic extracts interacted with hERα and hERβ, respectively, with a higher relative estrogenic potency with hERβ than with hERα. Antiestrogenic activity of the plant extracts was also determined by incubation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: YES assay; Estrogen receptor α (ERα); Estrogen receptor β (ERβ); Phytoestrogen; Isoflavonoid; Pueraria mirifica.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200010

Effects of central imidazolinergic and alpha2-adrenergic activation on water intake
Provedor de dados: 56
Autores: Sugawara,A.M.; Miguel,T.T.; Pereira,D.T.B.; Menani,J.V.; De Luca Jr.,L.A.
Non-adrenergic ligands that bind to imidazoline receptors (I-R), a selective ligand that binds to alpha2-adrenoceptors (alpha2-AR) and mixed ligands that bind to both receptors were tested for their action on water intake behavior of 24-h water-deprived rats. All drugs were injected into the third cerebral ventricle. Except for agmatine (80 nmol), mixed ligands binding to I-R/alpha2-AR such as guanabenz (40 nmol) and UK 14304 (20 nmol) inhibited water intake by 65% and up to 95%, respectively. The selective non-imidazoline alpha2-AR agonist, alpha-methylnoradrenaline, produced inhibition of water intake similar to that obtained with guanabenz, but at higher doses (80 nmol). The non-adrenergic I-R ligands histamine (160 nmol, mixed histaminergic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Imidazolines; Agmatine; UK 14304; Guanabenz; Alpha-methylnoradrenaline; Thirst; Dehydration.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900012

Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats
Provedor de dados: 56
Autores: Barros,M.A.V.; Andrade,E.B.; Barros,R.G.N.; Costa,I.K.M.; Costa,I.C.L.; Vitorino,G.F.A.; Andrade,J.J.C.; Paulino-Silva,K.M.; Nogueira,V.O.; de Brito Alves,J.L.; Costa-Silva,J.H.
The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, n=14) diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. The pregnant rats were then anesthetized and blood samples were collected for biochemical-hematological analyses, and laparotomy was performed to evaluate reproductive parameters. No sign of toxicity, or differences (P&gt;0.05) in body weight gain and biochemical parameters (urea, creatinine, albumin, globulin, and total protein) between NP and LP pregnant dams were observed. Similarly,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Development; Epigenetics; Fertility; Gestation; Intrauterine growth; Nutrition.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600613

Differences in synthesis and absorption of cholesterol of two effective lipid-lowering therapies
Provedor de dados: 56
Autores: Kasmas,S.H.; Izar,M.C.; França,C.N.; Ramos,S.C.; Moreira,F.T.; Helfenstein,T.; Moreno,R.A.; Borges,N.C.; Figueiredo-Neto,A.M.; Fonseca,F.A.
Effective statin therapy is associated with a marked reduction of cardiovascular events. However, the explanation for full benefits obtained for LDL cholesterol targets by combined lipid-lowering therapy is controversial. Our study compared the effects of two equally effective lipid-lowering strategies on markers of cholesterol synthesis and absorption. A prospective, open label, randomized, parallel design study, with blinded endpoints, included 116 subjects. We compared the effects of a 12-week treatment with 40 mg rosuvastatin or the combination of 40 mg simvastatin/10 mg ezetimibe on markers of cholesterol absorption (campesterol and β-sitosterol), synthesis (desmosterol), and their ratios to cholesterol. Both therapies similarly decreased total and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campesterol; Β-sitosterol; Desmosterol; Statin; Ezetimibe.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100015

Mechanisms of RON-mediated epithelial-mesenchymal transition in MDCK cells through the MAPK pathway
Provedor de dados: 56
Autores: Xiangming,Xu; Yun,Qian; Guoliang,Zhang; Jianjiang,Lin; Lisong,Teng.
The epithelial-mesenchymal transition (EMT) is involved in neoplastic metastasis, and the RON protein may be involved. In the present study, we determined the role and the mechanisms of action of RON in EMT in Madin-Darby canine kidney (MDCK) cells by Western blot and cell migration analysis. Activation of RON by macrophage stimulating protein (MSP) results in cell migration and initiates changes in the morphology of RON-cDNA-transfected MDCK cells. The absence of E-cadherin, the presence of vimentin and an increase in Snail were observed in RE7 cells, which were derived from MDCK cells transfected with wt-RON, compared with MDCK cells. Stimulation of RE7 cells with MSP resulted in increased migration (about 69% of the wounded areas were covered) as well...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RON; Epithelial-mesenchymal transition; MAPK; Erk.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700004

Thyroid peroxidase activity is inhibited by amino acids
Provedor de dados: 56
Autores: Carvalho,D.P.; Ferreira,A.C.F.; Coelho,S.M.; Moraes,J.M.; Camacho,M.A.S.; Rosenthal,D.
Normal in vitro thyroid peroxidase (TPO) iodide oxidation activity was completely inhibited by a hydrolyzed TPO preparation (0.15 mg/ml) or hydrolyzed bovine serum albumin (BSA, 0.2 mg/ml). A pancreatic hydrolysate of casein (trypticase peptone, 0.1 mg/ml) and some amino acids (cysteine, tryptophan and methionine, 50 µM each) also inhibited the TPO iodide oxidation reaction completely, whereas casamino acids (0.1 mg/ml), and tyrosine, phenylalanine and histidine (50 µM each) inhibited the TPO reaction by 54% or less. A pancreatic digest of gelatin (0.1 mg/ml) or any other amino acid (50 µM) tested did not significantly decrease TPO activity. The amino acids that impair iodide oxidation also inhibit the TPO albumin iodination activity. The inhibitory amino...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid; Thyroperoxidase; Amino acids; Iodide oxidation; Lactoperoxidase.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300015

Impact of chronic administration of anabolic androgenic steroids and taurine on blood pressure in rats
Provedor de dados: 56
Autores: Roşca,A.E.; Stoian,I.; Badiu,C.; Gaman,L.; Popescu,B.O.; Iosif,L.; Mirica,R.; Tivig,I.C.; Stancu,C.S.; Căruntu,C.; Voiculescu,S.E.; Zăgrean,L.
Supraphysiological administration of anabolic androgenic steroids has been linked to increased blood pressure. The widely distributed amino acid taurine seems to be an effective depressor agent in drug-induced hypertension. The purpose of this study was to assess the impact of chronic high dose administration of nandrolone decanoate (DECA) and taurine on blood pressure in rats and to verify the potentially involved mechanisms. The study was conducted in 4 groups of 8 adult male Wistar rats, aged 14 weeks, treated for 12 weeks with: DECA (A group); vehicle (C group); taurine (T group), or with both drugs (AT group). Systolic blood pressure (SBP) was measured at the beginning of the study (SBP1), 2 (SBP2) and 3 months (SBP3) later. Plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anabolic androgenic steroids; Nandrolone decanoate; Taurine; Blood pressure; Angiotensin-converting enzyme; Nitric oxide.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600706

Modification of pyruvate kinase and lactate dehydrogenase in foot muscle of the sea mussel Mytilus galloprovincialis under anaerobiosis and recovery
Provedor de dados: 56
The modification of pyruvate kinase (PK) and lactate dehydrogenase (LDH) activity in foot muscle of the mussel Mytilus galloprovincialis during exposure to air and recovery in water was investigated. In the course of exposure to air, the activity of these enzymes measured at high and low substrate concentrations showed successive increases and decreases. Returning the mussels to water after exposure to air affected enzyme activity in a manner similar to anaerobiosis. When measuring at saturated concentrations of substrates and substrate and coenzyme for PK and LDH, respectively, the maximum activation of PK (37%) was observed at 4 h of animal exposure to air, and for LDH (67%) at 6 h exposure to air. During 24 h of exposure of animals to air, PK activity...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mytilus galloprovincialis; Muscle; Anaerobiosis; Pyruvate kinase; Lactate dehydrogenase; Property modification.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300012

Blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in patients with symptoms suggesting reactive hypoglycemia
Provedor de dados: 56
Autores: Eik Filho,W.; Marcon,S.S.; Krupek,T.; Previdelli,I.T.S.; Pereira,O.C.N.; Silva,M.A.R.C.P.; Bazotte,R.B.
We evaluated the impact of postprandial glycemia on blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in non-diabetic patients with symptoms suggesting reactive hypoglycemia. Eleven patients with clinical symptoms suggesting reactive hypoglycemia received an oral glucose solution (75 g) Blood was collected at 0 (baseline), 30, 60, 120 and 180 min after glucose ingestion and the plasma concentrations of interferon-α (IFN-α), interferon-γ (IFN-γ), interleukin-1 receptor antagonist (IL-1RA), interleukin 2 (IL-2), interleukin-2 receptor (IL-2R), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 10 (IL-10), interleukin-12 (IL-12), interleukin 13 (IL-13), interleukin 15 (IL-15),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pro-inflammatory cytokines; Anti-inflammatory cytokines; Glucose tolerance test; Inflammation; Postprandial glycemia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800702

Fat gain with physical detraining is correlated with increased glucose transport and oxidation in periepididymal white adipose tissue in rats
Provedor de dados: 56
Autores: Sertié,R.A.L.; Andreotti,S.; Proença,A.R.G.; Campaña,A.B.; Lima,F.B.
As it is a common observation that obesity tends to occur after discontinuation of exercise, we investigated how white adipocytes isolated from the periepididymal fat of animals with interrupted physical training transport and oxidize glucose, and whether these adaptations support the weight regain seen after 4 weeks of physical detraining. Male Wistar rats (45 days old, weighing 200 g) were divided into two groups (n=10): group D (detrained), trained for 8 weeks and detrained for 4 weeks; and group S (sedentary). The physical exercise was carried out on a treadmill for 60 min/day, 5 days/week for 8 weeks, at 50-60% of the maximum running capacity. After the training protocol, adipocytes isolated from the periepididymal adipose tissue were submitted to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physical detraining; Adipocytes; Glucose uptake; Glucose oxidation; Lipogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700650

An experimental model of mycobacterial infection under corneal flaps
Provedor de dados: 56
Autores: Adan,C.B.D.; Sato,E.H.; Sousa,L.B.; Oliveira,R.S.; Leão,S.C.; Freitas,D.
In order to develop a new experimental animal model of infection with Mycobacterium chelonae in keratomileusis, we conducted a double-blind prospective study on 24 adult male New Zealand rabbits. One eye of each rabbit was submitted to automatic lamellar keratotomy with the automatic corneal shaper under general anesthesia. Eyes were immunosuppressed by a single local injection of methyl prednisolone. Twelve animals were inoculated into the keratomileusis interface with 1 µl of 10(6) heat-inactivated bacteria (heat-inactivated inoculum controls) and 12 with 1 µl of 10(6) live bacteria. Trimethoprim drops (0.1%, w/v) were used as prophylaxis for the surgical procedure every 4 h (50 µl, qid). Animals were examined by 2 observers under a slit lamp on the 1st,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious eye diseases; Keratitis; Animal model; Mycobacterium chelonae; Lamellar keratotomy.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700010

Inflammatory biomarkers responses after acute whole body vibration in fibromyalgia
Provedor de dados: 56
Autores: Ribeiro,V.G.C.; Mendonça,V.A.; Souza,A.L.C.; Fonseca,S.F.; Camargos,A.C.R.; Lage,V.K.S.; Neves,C.D.C.; Santos,J.M.; Teixeira,L.A.C.; Vieira,E.L.M.; Teixeira Junior,A.L.; Mezêncio,B.; Fernandes,J.S.C.; Leite,H.R.; Poortmans,J.R.; Lacerda,A.C.R.
The aims of this study were 1) to characterize the intensity of the vibration stimulation in women diagnosed with fibromyalgia (FM) compared to a control group of healthy women (HW) matched by age and anthropometric parameters, and 2) to investigate the effect of a single session of whole body vibration (WBV) on inflammatory responses. Levels of adipokines, soluble tumor necrosis factor receptors (sTNFr1, sTNFr2), and brain-derived neurotrophic factor (BDNF) were determined by enzyme-linked immunosorbent assay. Oxygen consumption (VO2) was estimated by a portable gas analysis system, heart rate (HR) was measured using a HR monitor, and perceived exertion (RPE) was evaluated using the Borg scale of perceived exertion. Acutely mild WBV increased VO2 and HR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomarkers; Fibromyalgia; Clinical trial; Inflammation; Acute exercise.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400615

Sequence analysis of the 5′ third of glycoprotein C gene of South American bovine herpesviruses 1 and 5
Provedor de dados: 56
Autores: Traesel,C.K.; Bernardes,L.M.; Spilki,F.R.; Weiblen,R.; Flores,E.F.
Bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) share high genetic and antigenic similarities, but exhibit marked differences in tissue tropism and neurovirulence. The amino-terminal region of glycoprotein C (gC), which is markedly different in each of the viruses, is involved in virus binding to cellular receptors and in interactions with the immune system. This study investigated the genetic and antigenic differences of the 5′ region of the gC (5′ gC) gene (amino-terminal) of South American BoHV-1 (n=19) and BoHV-5 (n=25) isolates. Sequence alignments of 374 nucleotides (104 amino acids) revealed mean similarity levels of 97.3 and 94.2% among BoHV-1 gC (gC1), respectively, 96.8 and 95.6% among BoHV-5 gC (gC5), and 62 and 53.3% between gC1 and gC5....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GC amino-terminal; BoHV; Genetic diversity; Epitope prediction; Virus neutralization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500470

Down-regulation of single-stranded DNA-binding protein 1 expression induced by HCMV infection promotes lipid accumulation in cells
Provedor de dados: 56
Autores: Guo,N.; Zhang,N.; Yan,L.; Cao,X.; Lv,F.; Wang,J.; Wang,Y.; Cong,H.
The objective of this study was to observe the infection of human cytomegalovirus (HCMV) to human umbilical vein endothelial cells, and its effect on the expression of single-stranded DNA-binding protein (SSBP1) and on lipid metabolism in endothelial cells. We screened the differential expression of mRNAs after HCMV infection by suppression subtractive hybridization and the expression levels of SSBP1 mRNA and protein after HCMV infection by real-time PCR and western blot. After verification of successful infection by indirect immunofluorescent staining and RT-PCR, we found a differential expression of lipid metabolism-related genes including LDLR, SCARB, CETP, HMGCR, ApoB and LPL induced by HCMV infection. The expression levels of SSBP1 mRNA and protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCMV; SSBP1; HUVEC; Lipid accumulation; LDLR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100605

Protective effect of dexmedetomidine against organ dysfunction in a two-hit model of hemorrhage/resuscitation and endotoxemia in rats
Provedor de dados: 56
Autores: Jiang,Yuanxu; Xia,Mingzhu; Huang,Qiang; Ding,Dengfeng; Li,Yali; Zhang,Zhongjun; Zhang,Xueping.
Dexmedetomidine (DEX), a selective agonist of α2-adrenergic receptors, has anti-inflammation properties and potential beneficial effects against trauma, shock, or infection. Therefore, this study aimed to investigate whether DEX might protect against multiple-organ dysfunction in a two-hit model of hemorrhage/resuscitation (HS) and subsequent endotoxemia. Eighty Wistar rats were randomized into four groups: NS (normal saline), HS/L (HS plus lipopolysaccharide), HS/L+D (HS/L plus dexmedetomidine), and HS/L+D+Y (HS/L+D plus yohimbine). Six hours after resuscitation, blood gas (PaO2) and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urine nitrogen (BUN), creatinine (Cr), TNF-α, IL-β, IL-6, IL-8, IL-10, and nitric oxide (NO)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemorrhagic shock; Resuscitation; Endotoxemia; Dexmedetomidine; Yohimbine.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300604

Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)
Provedor de dados: 56
Autores: Tanan,C.L.; Ventura,D.F.; de Souza,J.M.; Grotzner,S.R.; Mela,M.; Gouveia Jr.,A.; Oliveira-Ribeiro,C.A.
Methyl mercury (MeHg) is highly neurotoxic, affecting visual function in addition to other central nervous system functions. The effect of mercury intoxication on the amplitude of horizontal cell responses to light was studied in the retina of the fish Hoplias malabaricus. Intracellular responses were recorded from horizontal cells of fish previously intoxicated with MeHg by intraperitoneal injection (IP group) or by trophic exposure (T group). Only one retina per fish was used. The doses of MeHg chloride administered to the IP group were 0.01, 0.05, 0.1, 1.0, 2.0, and 6.0 mg/kg. The amplitudes of the horizontal cell responses were lower than control in individuals exposed to 0.01 (N = 4 retinas), 0.05 (N = 2 retinas) and 0.1 mg/kg (N = 1 retina), whereas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methyl mercury; Horizontal cells; Electrophysiology; Hoplias malabaricus; Retina.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700017

A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins
Provedor de dados: 56
Autores: Rocha,H.A.O.; Franco,C.R.C.; Trindade,E.S.; Carvalho,L.C.M.; Veiga,S.S.; Leite,E.L.; Dietrich,C.P.; Nader,H.B.
Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions. A fucan prepared from Spatoglossum schröederi was tested as a possible inhibitor of cell-matrix interactions using wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). The effect of this polymer on adhesion properties with specific extracellular matrix components was studied using several matrix proteins as substrates for cell attachment. Treatment with the polymer inhibited the adhesion of fibronectin to both CHO-K1 (2 x 10(5))()and CHO-745 (2 x 10(5) and 5 x 10(5))...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CHO cells; Extracellular matrix proteins; Fucan; Glycosaminoglycans; Cell adhesion; Brown algae.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500009

Prenatal and postnatal depression among low income Brazilian women
Provedor de dados: 56
Autores: Da-Silva,V.A.; Moraes-Santos,A.R.; Carvalho,M.S.; Martins,M.L.P.; Teixeira,N.A.
Postnatal depression is a significant problem affecting 10-15% of mothers in many countries and has been the subject of an increasing number of publications. Prenatal depression has been studied less. The aims of the present investigation were: 1) to obtain information on the prevalence of prenatal and postnatal depression in low income Brazilian women by using an instrument already employed in several countries, i.e., the Edinburgh Postnatal Depression Scale (EPDS); 2) to evaluate the risk factors involved in prenatal and postnatal depression in Brazil. The study groups included 33 pregnant women interviewed at home during the second and third trimesters of pregnancy, and once a month during the first six months after delivery. Questions on life events...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Depression; Pregnancy; Postnatal depression; Maternal depression.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600012

Thyrotropin and free thyroxine levels and coronary artery disease: cross-sectional analysis of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil)
Provedor de dados: 56
Autores: de Miranda,E.J.F. Peixoto; Bittencourt,M.S.; Staniak,H.L.; Sharovsky,R.; Pereira,A.C.; Foppa,M.; Santos,I.S.; Lotufo,P.A.; Benseñor,I.M.
Data on the association between subclinical thyroid dysfunction and coronary artery disease (CAD) is scarce. We aimed to analyze the association between thyroid function and CAD using baseline data from the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). We included subjects with normal thyroid function (0.4-4.0 mIU/L, and normal free thyroxine, FT4, or 0.8 to 1.9 ng/dL), subclinical hypothyroidism (SCHypo; TSH&gt;4.0 mIU/L and normal FT4), and subclinical hyperthyroidism (SCHyper; TSH&lt;0.4 mIU/L and normal FT4) evaluated by coronary computed tomography angiography. We excluded individuals using medications that interfere in thyroid function or with past medical history of cardiovascular disease. Logistic regression models evaluated the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid disorders; Thyrotropin levels; Coronary artery disease; Coronary computed tomography angiography; Subclinical atherosclerosis; Cardiovascular disease.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500609

The cardiopulmonary reflexes of spontaneously hypertensive rats are normalized after regression of left ventricular hypertrophy and hypertension
Provedor de dados: 56
Autores: Uggere,T.A.; Abreu,G.R.; Sampaio,K.N.; Cabral,A.M.; Bissoli,N.S.
Cardiopulmonary reflexes are activated via changes in cardiac filling pressure (volume-sensitive reflex) and chemical stimulation (chemosensitive reflex). The sensitivity of the cardiopulmonary reflexes to these stimuli is impaired in the spontaneously hypertensive rat (SHR) and other models of hypertension and is thought to be associated with cardiac hypertrophy. The present study investigated whether the sensitivity of the cardiopulmonary reflexes in SHR is restored when cardiac hypertrophy and hypertension are reduced by enalapril treatment. Untreated SHR and WKY rats were fed a normal diet. Another groups of rats were treated with enalapril (10 mg kg-1 day-1, mixed in the diet; SHRE or WKYE) for one month. After treatment, the volume-sensitive reflex...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Volume-sensitive reflex; Cardiac hypertrophy; Chemosensitive reflex; SHR; Enalapril.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500014

Serum uric acid and disorders of glucose metabolism: the role of glycosuria
Provedor de dados: 56
Autores: Andrade,J.A.M.; Kang,H.C.; Greffin,S.; Garcia Rosa,M.L.; Lugon,J.R.
Hyperuricemia has been associated with hypertension, diabetes mellitus, and metabolic syndrome. We studied the association between hyperuricemia and glycemic status in a nonrandomized sample of primary care patients. This was a cross-sectional study of adults ≥20 years old who were members of a community-based health care program. Hyperuricemia was defined as a value &gt;7.0 mg/dL for men and &gt;6.0 mg/dL for women. The sample comprised 720 participants including controls (n=257) and patients who were hypertensive and euglycemic (n=118), prediabetic (n=222), or diabetic (n=123). The mean age was 42.4±12.5 years, 45% were male, and 30% were white. The prevalence of hyperuricemia increased from controls (3.9%) to euglycemic hypertension (7.6%) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperuricemia; Glycosuria; Diabetes mellitus; Prediabetes.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000917

Induced maturation of hepatic progenitor cellsin vitro
Provedor de dados: 56
Autores: Bi,Y.; He,Y.; Huang,J.Y.; Xu,L.; Tang,N.; He,T.C.; Feng,T.
Hepatic progenitor cells (HPCs) are a potential cell source for liver cell transplantation but do not function like mature liver cells. We sought an effective and reliable method to induce HPC maturation. An immortalized HP14.5 albumin promoter-driven Gaussian luciferase (ALB-GLuc) cell line was established from HPCs isolated from fetal mouse liver of post coitus day 14.5 mice to investigate the effect of induction factors on ALB promoter. HP14.5 parental cells were cultured in DMEM with different combinations of 2% horse serum (HS), 0.1 µM dexamethasone (DEX), 10 ng/mL hepatic growth factor (HGF), and/or 20 ng/mL fibroblast growth factor 4 (FGF4). Trypan blue and crystal violet staining were used to assess cell proliferation with different induction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic progenitor cells; Induction; Maturation; Dexamethasone; Hepatic growth factor; Fibroblast growth factor 4.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700559

Procoagulant properties of human MV3 melanoma cells
Provedor de dados: 56
Autores: Geaquinto,D.L.A.; Fernandes,R.S.; Lima,L.G.; Barja-Fidalgo,C.; Monteiro,R.Q.
A correlation between cancer and prothrombotic states has long been described. More recently, a number of studies have focused on the procoagulant mechanisms exhibited by tumor cells. In the present study, we dissected the molecular mechanisms responsible for the procoagulant activity of MV3, a highly aggressive human melanoma cell line. It was observed that tumor cells strongly accelerate plasma coagulation as a result of: i) expression of the blood clotting initiator protein, a tissue factor, as shown by flow cytometry and functional assays (factor Xa formation in the presence of cells and factor VIIa), and ii) direct activation of prothrombin to thrombin by cells, as evidenced by hydrolysis of the synthetic substrate, S-2238, and the natural substrate,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MV3 melanoma; Blood clotting; Tissue factor.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200004

Eggplant (Solanum melongena) infusion has a modest and transitory effect on hypercholesterolemic subjects
Provedor de dados: 56
Autores: Guimarães,P.R.; Galvão,A.M.P.; Batista,C.M.; Azevedo,G.S.; Oliveira,R.D.; Lamounier,R.P.; Freire,N.; Barros,A.M.D.; Sakurai,E.; Oliveira,J.P.; Vieira,E.C.; Alvarez-Leite,J.I.
Eggplant (Solanum melongena) is consumed extensively in Brazil. It has been believed that infusion of a powdered preparation of the fruit may reduce serum cholesterol. However, there are few documented reports on its effects on cholesterol metabolism and its possible hypocholesterolemic effect has not been proved by well-controlled studies. The aim of the present study was to observe the effects of S. melongena on the serum cholesterol and triglycerides of 38 hypercholesterolemic human volunteers ingesting S. melongena infusion for five weeks. Thirty-eight hypercholesterolemic subjects receiving either S. melongena infusion (N = 19) or placebo (N = 19) participated in two clinical experiments in which the effect of S. melongena infusion was studied with (N...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholesterolemia; Eggplant; Diet; Solanum melongena; Atherosclerosis index.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900006

Role of sensory nervous system vasoactive peptides in hypertension
Provedor de dados: 56
Autores: Watson,R.E.; Supowit,S.C.; Zhao,H.; Katki,K.A.; DiPette,D.J.
The goal of the present research was to elucidate the roles and mechanisms by which the sensory nervous system, through the actions of potent vasodilator neuropeptides, regulates cardiovascular function in both the normal state and in the pathophysiology of hypertension. The animal models of acquired hypertension studied were deoxycorticosterone-salt (DOC-salt), subtotal nephrectomy-salt (SN-salt), and Nomega-nitro-L-arginine methyl ester (L-NAME)-induced hypertension during pregnancy in rats. The genetic model was the spontaneously hypertensive rat (SHR). Calcitonin gene-related peptide (CGRP) and substance P (SP) are potent vasodilating neuropeptides. In the acquired models of hypertension, CGRP and SP play compensatory roles to buffer the blood pressure...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Calcitonin gene-related peptide; Substance P; Neurons (afferent).
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900004

The role of T cell subsets and cytokines in the regulation of intracellular bacterial infection
Provedor de dados: 56
Autores: Oliveira,S.C.; Harms,J.S.; Rech,E.L.; Rodarte,R.S.; Bocca,A.L.; Goes,A.M.; Splitter,G.A.
Cellular immune responses are a critical part of the host's defense against intracellular bacterial infections. Immunity to Brucella abortus crucially depends on antigen-specific T cell-mediated activation of macrophages, which are the major effectors of cell-mediated killing of this organism. T lymphocytes that proliferate in response to B. abortus were characterized for phenotype and cytokine activity. Human, murine, and bovine T lymphocytes exhibited a type 1 cytokine profile, suggesting an analogous immune response in these different hosts. In vivo protection afforded by a particular cell type is dependent on the antigen presented and the mechanism of antigen presentation. Studies using MHC class I and class II knockout mice infected with B. abortus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: T cell subsets; Brucella abortus; Knockout mice; Cytokines; Intracellular pathogens; Genetic immunization.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100010

Autoantibody production in chronic idiopathic urticaria is not associated with Helicobacter pylori infection
Provedor de dados: 56
Autores: Atta,A.M.; Rodrigues,M.Z.A.; Sousa,C.P.; Medeiros Júnior,M.; Sousa-Atta,M.L.B.
Chronic idiopathic urticaria (CIU) is a dermatological syndrome, characterized by raised erythematous skin lesions, that affects 20% of the general population and has been associated with autoimmunity. However, some reports have also suggested a close relationship between CIU and Helicobacter pylori infection, which is endemic in developing countries and associated with chronic gastritis, peptic ulcer disease, and gastric carcinoma. In the present study, we investigated the occurrence of autoantibodies in sera from 23 CIU subjects infected with H. pylori and from 23 CIU subjects without this infection. The presence of anti-thyroid antibodies was determined by indirect hemagglutination assay and the presence of autoantibodies to IgE and C1INH was determined...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chronic idiopathic urticaria; Autoimmunity; Autoantibodies; Helicobacter pylori.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100002

A receptor for infectious and cellular prion protein
Provedor de dados: 56
Autores: Martins,V.R.
Prions are an unconventional form of infectious agents composed only of protein and involved in transmissible spongiform encephalopathies in humans and animals. The infectious particle is composed by PrPsc which is an isoform of a normal cellular glycosyl-phosphatidylinositol (GPI) anchored protein, PrPc, of unknown function. The two proteins differ only in conformation, PrPc is composed of 40% &lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt; helix while PrPsc has 60% ß-sheet and 20% &lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt; helix structure. The infection mechanism is trigged by interaction of PrPsc with cellular prion protein causing conversion of the latter's conformation. Therefore, the infection spreads because new PrPsc molecules are generated exponentially...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prion; Transmissible spongiform encephalopathies; PrPc; Receptor.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700009

Toxicity of a cyanobacterial extract containing microcystins to mouse lungs
Provedor de dados: 56
Autores: Picanço,M.R.; Soares,R.M.; Cagido,V.R.; Azevedo,S.M.F.O.; Rocco,P.R.M.; Zin,W.A.
Toxic cyanobacteria in drinking water supplies can cause serious public health problems. In the present study we analyzed the time course of changes in lung histology in young and adult male Swiss mice injected intraperitoneally (ip) with a cyanobacterial extract containing the hepatotoxic microcystins. Microcystins are cyclical heptapeptides quantified by ELISA method. Ninety mice were divided into two groups. Group C received an injection of saline (300 µl, ip) and group Ci received a sublethal dose of microcystins (48.2 µg/kg, ip). Mice of the Ci group were further divided into young (4 weeks old) and adult (12 weeks old) animals. At 2 and 8 h and at 1, 2, 3, and 4 days after the injection of the toxic cyanobacterial extract, the mice were anesthetized...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Microcystins; Lung; Inflammation; Alveolar collapse.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800013

Two related thrombin-like enzymes present in Bothrops atrox venom
Provedor de dados: 56
Autores: Petretski,J.H.; Kanashiro,M.; Silva,C.P.; Alves,E.W.; Kipnis,T.L.
This article describes the presence of two new forms of a thrombin-like enzyme, both with apparent molecular masses of 38 kDa, in Bothrops atrox venom. Both share the ability to cleave fibrinogen into fibrin and to digest casein. Both present identical Km on the substrate BApNA. Their N-terminal amino acid sequences are identical for 26 residues, sharing 80% homology with batroxobin and flavoxobin. Two groups of monoclonal antibodies (mAbs) raised against the purified enzyme forms recognized different epitopes of the putative corresponding enzymes present in B. atrox crude venom. On Western blotting analysis of B. atrox crude venom, mAbs 5DB2C8, 5AA10 and 5CF11, but not mAbs 6CC5 and 6AD2-G5, revealed two or more protein bands ranging from 25 to 38 kDa. By...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venoms; Bothrops atrox; Enzymes; Thrombin-like enzymes; Blood coagulation system.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100005

Rapid eye movement sleep deprivation induces an increase in acetylcholinesterase activity in discrete rat brain regions
Provedor de dados: 56
Autores: Benedito,M.A.C.; Camarini,R.
Some upper brainstem cholinergic neurons (pedunculopontine and laterodorsal tegmental nuclei) are involved in the generation of rapid eye movement (REM) sleep and project rostrally to the thalamus and caudally to the medulla oblongata. A previous report showed that 96 h of REM sleep deprivation in rats induced an increase in the activity of brainstem acetylcholinesterase (Achase), the enzyme which inactivates acetylcholine (Ach) in the synaptic cleft. There was no change in the enzyme's activity in the whole brain and cerebrum. The components of the cholinergic synaptic endings (for example, Achase) are not uniformly distributed throughout the discrete regions of the brain. In order to detect possible regional changes we measured Achase activity in several...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: REM sleep deprivation; Acetylcholinesterase; Brain regions; Thalamus; Medulla oblongata; Pons.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100012

Sexual behavior and fertility of male rats submitted to prolonged immobilization-induced stress
Provedor de dados: 56
Autores: Almeida,S.A.; Kempinas,W.G.; Lamano Carvalho,T.L.
In order to investigate whether prolonged stress interferes with the onset of sexual behavior at puberty and with fertility at adulthood, prepubertal male Wistar rats (40 days of age) were immobilized 6 h a day for 15 days (up to early puberty) or for 60 days (until sexual maturity). Pubertal stressed rats showed a two-fold increase in the latency for the first mount (probably due to repeated aversive experience in which a change of environment was always followed by immobilization) and a 2.5-fold increase in the frequency of thrusting (indicative of enhanced sexual performance). The apparently stimulatory effect of prolonged stress on the onset of sexual behavior is discussed in terms of increased testosterone level and interference with the complex...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization stress; Sexual behavior; Male fertility.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900019

Serum regucalcin is a useful indicator of liver injury severity in patients with hepatitis B virus-related liver diseases
Provedor de dados: 56
Autores: Wei,Xinhuan; Yu,Haibin; Zhao,Peng; Xie,Li; Li,Li; Zhang,Jing.
Regucalcin is a soluble protein that is principally expressed in hepatocytes. Studies of regucalcin have mainly been conducted in animals due to a lack of commercially available kits. We aimed to develop an enzyme-linked immunosorbent assay (ELISA) to quantify serum regucalcin in patients with hepatitis B virus (HBV)-related disease. High-titer monoclonal antibodies and a polyclonal antibody to regucalcin were produced, a double-antibody sandwich ELISA method was established, and serum regucalcin was determined in 47 chronic hepatitis B (CHB) patients, 91 HBV-related acute-on-chronic liver failure (HBV-ACLF) patients, and 33 healthy controls. The ELISA demonstrated an appropriate linear range, and high levels of reproducibility, sensitivity, specificity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Regucalcin; Liver injury; Chronic hepatitis B infection; ELISA; Liver failure.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000605

Lack of evidence for mutations or deletions in the CDKN2A/p16 and CDKN2B/p15 genes of Brazilian neuroblastoma patients
Provedor de dados: 56
Autores: Bassi,C.L.; Martelli,L.; Cipolotti,R.; Scrideli,C.A.; Defávery,R.; Tone,L.G.
Neuroblastoma, the most common extracranial tumor in childhood, has a wide spectrum of clinical and biological features. The loss of heterozygosity within the 9p21 region has been reported as a prognostic factor. Two tumor suppressor genes located in this region, the CDKN2B/p15 and CDKN2A/p16 (cyclin-dependent kinase inhibitors 2B and 2A, respectively) genes, play a critical role in cell cycle progression and are considered to be targets for tumor inactivation. We analyzed CDKN2B/p15 and CDKN2A/p16 gene alterations in 11 patients, who ranged in age from 4 months to 13 years (male/female ratio was 1.2:1). The most frequent stage of the tumor was stage IV (50%), followed by stages II and III (20%) and stage I (10%). The samples were submitted to the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: P15 gene; P16 gene; Deletion; Mutation; Neuroblastoma.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100014

Evaluation of electromyographic activity and heart rate responses to isometric exercise. The role played by muscular mass and type
Provedor de dados: 56
Autores: Silva,E.; Oliveira,L.; Catai,A.M.; Ferreira-Filho,P.; Bérzin,F.; Gallo Jr.,L.
The purpose of the present study was to examine the relationship between the electromyographic (EMG) activity and heart rate (HR) responses induced by isometric exercise performed by knee extension (KE) and flexion (KF) in men. Fifteen healthy male subjects, 21 ± 1.3 years (mean ± SD), were submitted to KE and KF isometric exercise tests at 100% of maximal voluntary contraction (MVC). The exercises were performed with one leg (right or left) and with two legs simultaneously, for 10 s in the sitting position with the hip and knee flexed at 90o. EMG activity (root mean square values) and HR (beats/min) were recorded simultaneously both at rest and throughout the sustained contraction. The HR responses to isometric exercise in KE and KF were similar when...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Knee; Isometric exercise; Heart rate; Electromyography; Rectus femoris; Biceps femoris.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100017

Efficacy of the dietary histone deacetylase inhibitor butyrate alone or in combination with vitamin A against proliferation of MCF-7 human breast cancer cells
Provedor de dados: 56
Autores: Andrade,F.O.; Nagamine,M.K.; Conti,A. De; Chaible,L.M.; Fontelles,C.C.; Jordão Junior,A.A.; Vannucchi,H.; Dagli,M.L.Z.; Bassoli,B.K.; Moreno,F.S.; Ong,T.P.
The combined treatment with histone deacetylase inhibitors (HDACi) and retinoids has been suggested as a potential epigenetic strategy for the control of cancer. In the present study, we investigated the effects of treatment with butyrate, a dietary HDACi, combined with vitamin A on MCF-7 human breast cancer cells. Cell proliferation was evaluated by the crystal violet staining method. MCF-7 cells were plated at 5 x 10(4) cells/mL and treated with butyrate (1 mM) alone or combined with vitamin A (10 µM) for 24 to 120 h. Cell proliferation inhibition was 34, 10 and 46% following treatment with butyrate, vitamin A and their combination, respectively, suggesting that vitamin A potentiated the inhibitory activities of butyrate. Furthermore, exposure to this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; MCF-7 cells; Butyric acid; Vitamin A; Epigenetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900008

Adrenoceptors of the medial septal area modulate water intake and renal excretory function induced by central administration of angiotensin II
Provedor de dados: 56
Autores: Saad,W.A.; Guarda,I.F.M.S.; Camargo,L.A.A.; Santos,T.A.F.B.; Simões,S.; Saad,Willian A.
We investigated the role of a-adrenergic antagonists and clonidine injected into the medial septal area (MSA) on water intake and the decrease in Na+, K+ and urine elicited by ANGII injection into the third ventricle (3rdV). Male Holtzman rats with stainless steel cannulas implanted into the 3rdV and MSA were used. ANGII (12 nmol/µl) increased water intake (12.5 ± 1.7 ml/120 min). Clonidine (20 nmol/µl) injected into the MSA reduced the ANGII-induced water intake (2.9 ± 0.5 ml/120 min). Pretreatment with 80 nmol/µl yohimbine or prazosin into the MSA also reduced the ANGII-induced water intake (3.0 ± 0.4 and 3.1 ± 0.2 ml/120 min, respectively). Yohimbine + prazosin + clonidine injected into the MSA abolished the ANGII-induced water intake (0.2 ± 0.1 and 0.2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: A-Adrenoceptors; Angiotensin II; Water intake; Sodium; Potassium; Water excretion; Medial septal area.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800012

Protein dynamics: hydration and cavities
Provedor de dados: 56
Autores: Heremans,K.
The temperature-pressure behavior of proteins seems to be unique among the biological macromolecules. Thermodynamic as well as kinetic data show the typical elliptical stability diagram. This may be extended by assuming that the unfolded state gives rise to volume and enthalpy-driven liquid-liquid transitions. A molecular interpretation follows from the temperature and the pressure dependence of the hydration and cavities. We suggest that positron annihilation spectroscopy can provide additional quantitative evidence for the contributions of cavities to the dynamics of proteins. Only mature amyloid fibrils that form from unfolded proteins are very resistant to pressure treatment.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stability diagram; Thermodynamics; High pressure; Unfolding; Amyloid fibrils; Protein dynamics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800002

Lack of evidence for superantigen activity of Toxoplasma gondii towards human T cells
Provedor de dados: 56
Autores: Vallochi,A.L.; Yamamoto,J.H.; Schlesinger,D.; Machado,M.A.C.; Silveira,C.; Martins,M.C.; Belfort Jr.,R.; Kalil,J.; Rizzo,L.V.
Toxoplasma gondii is an obligatory intracellular parasite whose life cycle may include man as an intermediate host. More than 500 million people are infected with this parasite worldwide. It has been previously reported that T. gondii contains a superantigen activity. The purpose of the present study was to determine if the putative superantigen activity of T. gondii would manifest towards human T cells. Peripheral blood mononuclear cells (PBMC) from individuals with no previous contact with the parasite were evaluated for proliferation as well as specific Vß expansion after exposure to Toxoplasma antigens. Likewise, PBMC from individuals with the congenital infection were evaluated for putative Vß family deletions in their T cell repertoire. We also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma; Tolerance; Superantigen; T cell receptor.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800008

Gut permeability to lactulose and mannitol differs in treated Crohn's disease and celiac disease patients and healthy subjects
Provedor de dados: 56
Autores: Vilela,E.G.; Torres,H.O.G.; Ferrari,M.L.A.; Lima,A.S.; Cunha,A.S.
The gut barrier monitors and protects the gastrointestinal tract from challenges such as microorganisms, toxins and proteins that could act as antigens. There is evidence that gut barrier dysfunction may act as a primary disease mechanism in intestinal disorders. The aim of the present study was to evaluate the barrier function towards sugars after the appropriate treatment of celiac disease and Crohn's disease patients and compare the results with those obtained with healthy subjects. Fifteen healthy volunteers, 22 celiac disease patients after 1 year of a gluten-free diet, and 31 Crohn's disease patients in remission were submitted to an intestinal permeability test with 6.0 g lactulose and 3.0 g mannitol. Six-hour urinary lactulose excretion in Crohn's...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal permeability; Celiac disease; Crohn's disease; Mannitol; Lactulose.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200010

Measurement of serum estrogen and estrogen metabolites in pre- and postmenopausal women with osteoarthritis using high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry
Provedor de dados: 56
Autores: Gao,W.L.; Wu,L.S.; Zi,J.H.; Wu,B.; Li,Y.Z.; Song,Y.C.; Cai,D.Z.
Although 17β-estradiol (E2) deficiency has been linked to the development of osteoarthritis (OA) in middle-aged women, there are few studies relating other estrogens and estrogen metabolites (EMs) to this condition. We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method to measure the levels of six EMs (i.e., estrone, E2, estriol, 2-hydroxyestrone, 2-hydroxyestradiol, and 16a-hydroxyestrone) in healthy pre- and postmenopausal women and women with OA. This method had a precision ranging from 1.1 to 3.1% and a detection limit ranging from 10 to 15 pg. Compared to healthy women, serum-free E2 was lower in the luteal and postmenopausal phases in women with OA, and total serum E2 was lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estrogen; Estrogen metabolites; Menopause; Serum; Osteoathritis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200146

Predicting pathway cross-talks in ankylosing spondylitis through investigating the interactions among pathways
Provedor de dados: 56
Autores: Gu,Xiang; Liu,Cong-Jian; Wei,Jian-Jie.
Given that the pathogenesis of ankylosing spondylitis (AS) remains unclear, the aim of this study was to detect the potentially functional pathway cross-talk in AS to further reveal the pathogenesis of this disease. Using microarray profile of AS and biological pathways as study objects, Monte Carlo cross-validation method was used to identify the significant pathway cross-talks. In the process of Monte Carlo cross-validation, all steps were iterated 50 times. For each run, detection of differentially expressed genes (DEGs) between two groups was conducted. The extraction of the potential disrupted pathways enriched by DEGs was then implemented. Subsequently, we established a discriminating score (DS) for each pathway pair according to the distribution of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ankylosing spondylitis; Monte Carlo cross-validation; Differentially expressed genes; Random forest classification; Pathway cross-talk.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100607

Effect of surgical treatment on the cellular immune response of gastric cancer patients
Provedor de dados: 56
Autores: Barbieri,C.; Fujisawa,M.M.; Yasuda,C.L.; Metze,I.L.; Oliveira,E.C.; Santos,L.M.B.; Lopes,L.R.; Andreollo,N.A.
Patients with gastric cancer have a variety of immunological abnormalities. In the present study the lymphocytes and their subsets were determined in the peripheral blood of patients with gastric cancer (N = 41) both before and after surgical treatment. The percent of helper/inducer CD4 T cells (43.6 ± 8.9) was not different after tumor resection (43.6 ± 8.2). The percent of the cytotoxic CD8+ T cell population decreased significantly, whether patients were treated surgically (27.2 ± 5.8%, N = 20) or not (27.3 ± 7.3%, N = 20) compared to individuals with inflammatory disease (30.9 ± 7.5%) or to healthy individuals (33.2 ± 7.6%). The CD4/CD8 ratio consequently increased in the group of cancer patients. The peripheral blood lymphocytes of gastric cancer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric cancer; Cellular immune response; Transforming growth factor beta; Immunosuppression.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300008

Antibodies against electronegative LDL inhibit atherosclerosis in LDLr-/- mice
Provedor de dados: 56
Autores: Grosso,D.M.; Ferderbar,S.; Wanschel,A.C.B.A.; Krieger,M.H.; Higushi,M.L.; Abdalla,D.S.P.
In order to determine the effect of antibodies against electronegative low-density lipoprotein LDL(-) on atherogenesis, five groups of LDL low receptor-deficient (LDLr-/-) mice (6 per group) were immunized with the following antibodies (100 µg each): mouse anti-LDL(-) monoclonal IgG2b, rabbit anti-LDL(-) polyclonal IgG or its Fab fragments and mouse irrelevant monoclonal IgG and non-immunized controls. Antibodies were administered intravenously one week before starting the hypercholesterolemic diet (1.25% cholesterol) and then every week for 21 days. The passive immunization with anti-LDL(-) monoclonal IgG2b, polyclonal antibody and its derived Fab significantly reduced the cross-sectional area of atherosclerotic lesions at the aortic root of LDLr-/- mice...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Minimally modified LDL; Passive immunization; Monoclonal antibody; Atherogenesis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200007

Estimating the average length of hospitalization due to pneumonia: a fuzzy approach
Provedor de dados: 56
Autores: Nascimento,L.F.C.; Rizol,P.M.S.R.; Peneluppi,A.P.
Exposure to air pollutants is associated with hospitalizations due to pneumonia in children. We hypothesized the length of hospitalization due to pneumonia may be dependent on air pollutant concentrations. Therefore, we built a computational model using fuzzy logic tools to predict the mean time of hospitalization due to pneumonia in children living in São José dos Campos, SP, Brazil. The model was built with four inputs related to pollutant concentrations and effective temperature, and the output was related to the mean length of hospitalization. Each input had two membership functions and the output had four membership functions, generating 16 rules. The model was validated against real data, and a receiver operating characteristic (ROC) curve was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Air pollutants; Fuzzy logic; Pneumonia; Particulate matter; Sulfur dioxide.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100977

Detection of multiple mycoplasma infection in cell cultures by PCR
Provedor de dados: 56
Autores: Timenetsky,J.; Santos,L.M.; Buzinhani,M.; Mettifogo,E.
A total of 301 cell cultures from 15 laboratories were monitored for mycoplasma (Mollicutes) using PCR and culture methodology. The infection was detected in the cell culture collection of 12 laboratories. PCR for Mollicutes detected these bacteria in 93 (30.9%) samples. Although the infection was confirmed by culture for 69 (22.9%) samples, PCR with generic primers did not detect the infection in five (5.4%). Mycoplasma species were identified with specific primers in 91 (30.2%) of the 98 samples (32.6%) considered to be infected. Mycoplasma hyorhinis was detected in 63.3% of the infected samples, M. arginini in 59.2%, Acholeplasma laidlawii in 20.4%, M. fermentans in 14.3%, M. orale in 11.2%, and M. salivarium in 8.2%. Sixty (61.2%) samples were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Cell culture; Multiple infection; Mycoplasma detection.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700009

Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
Provedor de dados: 56
Autores: Merjan,A.J.; Kanashiro,C.A.; Krieger,J.E.; Han,S.W.; Paiva,A.C.M.
A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; Agonist-induced receptor internalization; Endocytosis; Fluorescence-tagged receptor; Nuclear angiotensin receptors; Renin-angiotensin system.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900011

Rosai-Dorfman disease: a report of eight cases in a tertiary care center and a review of the literature
Provedor de dados: 56
Autores: Maia,R.C.; de Meis,E.; Romano,S.; Dobbin,J.A.; Klumb,C.E.
Rosai-Dorfman disease (RDD) is a nonmalignant histiocytic disorder of unknown origin that is extremely rare. By immunohistochemistry, the RDD cells are characteristically S-100 positive and CD1a negative. Emperipolesis is a common histopathological finding, although not specific for RDD. Lymph node and cutaneous manifestations are most frequent, but diverse organs can be affected. The clinical course is unpredictable regardless of treatment. Here, we present a series of 8 cases presenting lymph node and/or cutaneous lesions. Lymph node involvement was seen in diverse regions, including mediastinal and retroperitoneal. The treatment response to steroids was diversified, and the chemotherapy response was disappointing. Associated autoimmune diseases (Sjögren...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosai-Dorfman disease; Sinus histiocytosis with massive lymphadenopathy; Histiocytosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000100006

Naringenin regulates production of matrix metalloproteinases in the knee-joint and primary cultured articular chondrocytes and alleviates pain in rat osteoarthritis model
Provedor de dados: 56
Autores: Wang,C.C.; Guo,L.; Tian,F.D.; An,N.; Luo,L.; Hao,R.H.; Wang,B.; Zhou,Z.H.
Inflammation of cartilage is a primary symptom for knee-joint osteoarthritis. Matrix metalloproteinases (MMPs) are known to play an important role in the articular cartilage destruction related to osteoarthritis. Naringenin is a plant-derived flavonoid known for its anti-inflammatory properties. We studied the effect of naringenin on the transcriptional expression, secretion and enzymatic activity of MMP-3 in vivo in the murine monosodium iodoacetate (MIA) osteoarthritis model. The assessment of pain behavior was also performed in the MIA rats. The destruction of knee-joint tissues was analyzed microscopically. Moreover, the effect of naringenin was also studied in vitro in IL-1β activated articular chondrocytes. The transcriptional expression of MMP-3,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metalloproteinase; Osteoarthritis; Naringenin; Chondrocyte; Knee-joint; Rat MIA model.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400603

Decreased spermatogenic and androgenic testicular functions in adult rats submitted to immobilization-induced stress from prepuberty
Provedor de dados: 56
Autores: Almeida,S.A.; Petenusci,S.O.; Anselmo-Franci,J.A.; Rosa-e-Silva,A.A.M.; Lamano-Carvalho,T.L.
We investigated whether chronic stress applied from prepuberty to full sexual maturity interferes with spermatogenic and androgenic testicular functions. Male Wistar rats (40 days old) were immobilized 6 h a day for 60 days. Following immobilization, plasma concentrations of corticosterone and prolactin increased 135% and 48%, respectively, while plasma luteinizing hormone and testosterone presented a significant decrease of 29% and 37%, respectively. Plasma concentration of follicle-stimulating hormone was not altered in stressed rats. Chronic stress reduced the amount of mature spermatids in the testis by 16% and the spermatozoon concentration in the cauda epididymidis by 32%. A 17% reduction in weight and a 42% decrease in DNA content were observed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Spermatogenesis; LH; FSH; Testosterone; Prolactin.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100013

Cellular and matrix interactions during the development of T lymphocytes
Provedor de dados: 56
Autores: Owen,J.J.T.; McLoughlin,D.E.; Suniara,R.K.; Jenkinson,E.J.
The thymus contains an extensive extracellular matrix. Although thymocytes express integrins capable of binding to matrix molecules, the functional significance of the matrix for T cell development is uncertain. We have shown that the matrix is associated with thymic fibroblasts which are required for the CD44+ CD25+ stage of double negative (CD4-8-) thymocyte development. The survival of cells at this stage is dependent on IL-7 and we propose that the role of fibroblasts is to present, via the matrix, IL-7 to developing T cells.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Epithelial cells; Fibroblasts; Integrins; Interleukin-7; Thymus.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500008

Effects of bromopride on expression of metalloproteinases and interleukins in left colonic anastomoses: an experimental study
Provedor de dados: 56
Autores: Silva,S.M.; Jerônimo,M.S.; Silva-Pereira,I.; Bocca,A.L.; Sousa,J.B.
Anastomotic dehiscence is the most severe complication of colorectal surgery. Metalloproteinases (MMPs) and interleukins (ILs) can be used to analyze the healing process of anastomosis. To evaluate the effects of bromopride on MMP and cytokine gene expression in left colonic anastomoses in rats with or without induced abdominal sepsis, 80 rats were divided into two groups for euthanasia on the third or seventh postoperative day (POD). They were then divided into subgroups of 20 rats for sepsis induction or not, and then into subgroups of 10 rats for administration of bromopride or saline. Left colonic anastomosis was performed and abdominal sepsis was induced by cecal ligation and puncture. A colonic segment containing the anastomosis was removed for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromopride; Matrix metalloproteinases; Interleukins; Surgical anastomosis; Sepsis; Rats.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000911

Priming effects of a peripheral visual stimulus in simple and go/no-go tasks
Provedor de dados: 56
Autores: Squella,S.A.F.; Ribeiro-do-Valle,L.E.
The early facilitatory effect of a peripheral spatially visual prime stimulus described in the literature for simple reaction time tasks has been usually smaller than that described for complex (go/no-go, choice) reaction time tasks. In the present study we investigated the reason for this difference. In a first and a second experiment we tested the participants in both a simple task and a go/no-go task, half of them beginning with one of these tasks and half with the other one. We observed that the prime stimulus had an early effect, inhibitory for the simple task and facilitatory for the go/no-go task, when the task was performed first. No early effect appeared when the task was performed second. In a third and a fourth experiment the participants were,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Priming; Attention; Masking; Simple task; Go/no-go task.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200013

PCR identification of Mycobacterium tuberculosis complex in a clinical sample from a patient with symptoms of tuberculous spondylodiscitis
Provedor de dados: 56
Autores: Ribeiro,M.A.; Barouni,A.S.; Augusto,C.J.; Augusto,M.V.S.; Lopes,M.T.P.; Salas,C.E.
A 42-year-old male complaining of thoracic spine pain was admitted to the hospital for evaluation. An X-ray and computer tomography of the thoracic spine showed spondylodiscitis of the L3 lumbar and L2-L3 intervertebral disk. The tuberculin skin test (PPD) was strongly positive. A radioscopy-guided fine needle aspirate of the affected area was cultured but did not reveal the cause of the disease. Two biopsy attempts failed to reveal the cause of the disease by culturing or by acid-fast-resistant staining (Ziehl Neelsen) of the specimens. A third biopsy also failed to detect the infectious agent by using microbiological procedures, but revealed the presence of a 245-bp amplicon characteristic of the Mycobacterium tuberculosis complex after PCR of the...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Spondylodiscitis; Tuberculosis; Polymerase chain reaction; Mycobacterium tuberculosis complex.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100001

Short-term follow-up of exercise training program and beta-blocker treatment on quality of life in dogs with naturally acquired chronic mitral valve disease
Provedor de dados: 56
Autores: Marcondes-Santos,M.; Mansur,A.P.; Fragata,F.S.; Strunz,C.M.C.
This study aimed to evaluate the effects of carvedilol treatment and a regimen of supervised aerobic exercise training on quality of life and other clinical, echocardiographic, and biochemical variables in a group of client-owned dogs with chronic mitral valve disease (CMVD). Ten healthy dogs (control) and 36 CMVD dogs were studied, with the latter group divided into 3 subgroups. In addition to conventional treatment (benazepril, 0.3-0.5 mg/kg once a day, and digoxin, 0.0055 mg/kg twice daily), 13 dogs received exercise training (subgroup I; 10.3±2.1 years), 10 dogs received carvedilol (0.3 mg/kg twice daily) and exercise training (subgroup II; 10.8±1.7 years), and 13 dogs received only carvedilol (subgroup III; 10.9±2.1 years). All drugs were administered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic mitral valve disease; Beta-blocker; Exercise training; Dogs.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000004

Study of the comprehension of the scientific method by members of a university health research laboratory
Provedor de dados: 56
Autores: Burlamaque-Neto,A.C.; Santos,G.R.; Lisbôa,L.M.; Goldim,J.R.; Machado,C.L.B.; Matte,U.; Giugliani,R.
In Brazil, scientific research is carried out mainly at universities, where professors coordinate research projects with the active participation of undergraduate and graduate students. However, there is no formal program for the teaching/learning of the scientific method. The objective of the present study was to evaluate the comprehension of the scientific method by students of health sciences who participate in scientific projects in an academic research laboratory. An observational descriptive cross-sectional study was conducted using Edgar Morin complexity as theoretical reference. In a semi-structured interview, students were asked to solve an abstract logical puzzle - TanGram. The collected data were analyzed using the hermeneutic-dialectic analysis...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Scientific method; Complexity; Academic scientific research.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200001

Effect of lipoarabinomannan from Mycobacterium avium subsp avium in Freund’s incomplete adjuvant on the immune response of cattle
Provedor de dados: 56
Autores: Colavecchia,S.B.; Jolly,A.; Fernández,B.; Fontanals,A.M.; Fernández,E.; Mundo,S.L.
The aim of the present study was to determine whether lipoarabinomannan (LAM), in combination with Freund’s incomplete adjuvant (FIA), was able to improve cell-mediated and antibody-mediated immune responses against ovalbumin (OVA) in cattle. Twenty-three calves were assigned to four treatment groups, which were subcutaneously immunized with either OVA plus FIA, OVA plus FIA and LAM from Mycobacterium avium subsp avium, FIA plus LAM, or FIA alone. Lymphoproliferation, IFN-γ production and cell subpopulations on peripheral blood mononuclear cells before and 15 days after treatment were evaluated. Delayed hypersensitivity was evaluated on day 57. Specific humoral immune response was measured by ELISA. Inoculation with LAM induced higher levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipoarabinomannan; Cattle; Immunomodulation; Mycobacterium avium subsp avium.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200008

Absence of linkage between MHC and a gene involved in susceptibility to human schistosomiasis
Provedor de dados: 56
Autores: Chiarella,J.M.; Goldberg,A.C.; Abel,L.; Carvalho,E.M.; Kalil,J.; Dessein,A.
Six hundred million people are at risk of infection by Schistosoma mansoni. MHC haplotypes have been reported to segregate with susceptibility to schistosomiasis in murine models. In humans, a major gene related to susceptibility/resistance to infection by S. mansoni (SM1) and displaying the mean fecal egg count as phenotype was detected by segregation analysis. This gene displayed a codominant mode of inheritance with an estimated frequency of 0.20-0.25 for the deleterious allele and accounted for more than 50% of the variance of infection levels. To determine if the SM1 gene segregates with the human MHC chromosomal region, we performed a linkage study by the lod score method. We typed for HLA-A, B, C, DR and DQ antigens in 11 informative families from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA; Linkage study; Human schistosomiasis.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500010

Metabolomics as a promising tool for early osteoarthritis diagnosis
Provedor de dados: 56
Autores: de Sousa,E.B.; dos Santos Junior,G.C.; Duarte,M.E.L.; Moura Neto,V.; Aguiar,D.P.
Osteoarthritis (OA) is the main cause of disability worldwide, due to progressive articular cartilage loss and degeneration. According to recent research, OA is more than just a degenerative disease due to some metabolic components associated to its pathogenesis. However, no biomarker has been identified to detect this disease at early stages or to track its development. Metabolomics is an emerging field and has the potential to detect many metabolites in a single spectrum using high resolution nuclear magnetic resonance (NMR) techniques or mass spectrometry (MS). NMR is a reproducible and reliable non-destructive analytical method. On the other hand, MS has a lower detection limit and is more destructive, but it is more sensitive. NMR and MS are useful...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NMR; Metabolomics; Osteoarthritis; Synovial Fluid.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100301

High HTLV-1 proviral load, a marker for HTLV-1 associated myelopathy/tropical spastic paraparesis, is also detected in patients with infective dermatitis associated with HTLV-1
Provedor de dados: 56
Autores: Primo,J.; Siqueira,I.; Nascimento,M.C.F.; Oliveira,M.F.; Farre,L.; Carvalho,E.M.; Bittencourt,A.L.
Salvador (BA, Brazil) is an endemic area for human T-cell lymphotrophic virus type 1 (HTLV-1). The overall prevalence of HTLV-1 infection in the general population has been estimated to be 1.76%. HTLV-1 carriers may develop a variety of diseases such as adult T-cell leukemia/lymphoma, HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and infective dermatitis associated with HTLV-1 (IDH). IDH is a chronic and severe form of childhood exudative and infective dermatitis involving mainly the scalp, neck and ears. It has recently been observed that 30% of patients with IDH develop juvenile HAM/TSP. The replication of HTLV-1 has been reported to be greater in adult HAM/TSP patients than in asymptomatic HTLV-1 carriers. In the current study, the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Infective dermatitis associated with HTLV-1; HTLV-1 associated myelopathy/tropical spastic paraparesis; HTLV-1 proviral loads.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800012

Pleiotropic effects of simvastatin in physically trained ovariectomized rats
Provedor de dados: 56
Autores: Bernardes,N.; Brito,J.O.; Fernandes,T.G.; Llesuy,S.F.; Irigoyen,M.C.; Belló-Klein,A.; De Angelis,K.
This study tested the hypothesis that simvastatin treatment can improve cardiovascular and autonomic functions and membrane lipoperoxidation, with an increased effect when applied to physically trained ovariectomized rats. Ovariectomized rats were divided into sedentary, sedentary+simvastatin and trained+simvastatin groups (n = 8 each). Exercise training was performed on a treadmill for 8 weeks and simvastatin (5 mg/kg) was administered in the last 2 weeks. Blood pressure (BP) was recorded in conscious animals. Baroreflex sensitivity was evaluated by the tachycardic and bradycardic responses to BP changes. Cardiac vagal and sympathetic effects were determined using methylatropine and propranolol. Oxidative stress was evaluated based on heart and liver...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Menopause; Exercise training; Simvastatin; Autonomic function; Oxidative stress.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500447

Stress-related hormone norepinephrine induces interleukin-6 expression in GES-1 cells
Provedor de dados: 56
Autores: Yang,R.; Lin,Q.; Gao,H.B.; Zhang,P.
In the current literature, there is evidence that psychological factors can affect the incidence and progression of some cancers. Interleukin 6 (IL-6) is known to be elevated in individuals experiencing chronic stress and is also involved in oncogenesis and cancer progression. However, the precise mechanism of IL-6 induction by the stress-related hormone norepinephrine (NE) is not clear, and, furthermore, there are no reports about the effect of NE on IL-6 expression in gastric epithelial cells. In this study, we examined the effect of NE on IL-6 expression in immortalized human gastric epithelial cells (GES-1 cells). Using real-time PCR and enzyme-linked immunoassay, we demonstrated that NE can induce IL-6 mRNA and protein expression in GES-1 cells. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Norepinephrine; GES-1 cells; Interleukin-6.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200101

Chi-square analysis of the reduction of ATP levels in L-02 hepatocytes by hexavalent chromium
Provedor de dados: 56
Autores: Yuan,Yang; Peng,Li; Gong-Hua,Hu; Lu,Dai; Xia-Li,Zhong; Yu,Zhou; Cai-Gao,Zhong.
This study explored the reduction of adenosine triphosphate (ATP) levels in L-02 hepatocytes by hexavalent chromium (Cr(VI)) using chi-square analysis. Cells were treated with 2, 4, 8, 16, or 32 μM Cr(VI) for 12, 24, or 36 h. Methyl thiazolyl tetrazolium (MTT) experiments and measurements of intracellular ATP levels were performed by spectrophotometry or bioluminescence assays following Cr(VI) treatment. The chi-square test was used to determine the difference between cell survival rate and ATP levels. For the chi-square analysis, the results of the MTT or ATP experiments were transformed into a relative ratio with respect to the control (%). The relative ATP levels increased at 12 h, decreased at 24 h, and increased slightly again at 36 h following 4, 8,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hexavalent chromium; ATP level; Interference effect; Chi-square test.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600002

The role of necroptosis in neurosurgical diseases
Provedor de dados: 56
Autores: Liu,T.; Bao,Y.H.; Wang,Y.; Jiang,J.Y.
Programmed necrosis or necroptosis is an alternative form of cell death that is executed through a caspase-independent pathway. Necroptosis has been implicated in many pathological conditions. Genetic or pharmacological inhibition of necroptotic signaling has been shown to confer neuroprotection after traumatic and ischemic brain injury. Therefore, the necroptotic pathway represents a potential target for neurological diseases that are managed by neurosurgeons. In this review, we summarize recent advances in the understanding of necroptotic signaling pathways and explore the role of necroptotic cell death in craniocerebral trauma, brain tumors, and cerebrovascular diseases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular mechanism; Pathogenesis; Programmed cell death; Therapy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400292

The extracellular matrix provides directional cues for neuronal migration during cerebellar development
Provedor de dados: 56
Autores: Porcionatto,M.A.
Normal central nervous system development relies on accurate intrinsic cellular programs as well as on extrinsic informative cues provided by extracellular molecules. Migration of neuronal progenitors from defined proliferative zones to their final location is a key event during embryonic and postnatal development. Extracellular matrix components play important roles in these processes, and interactions between neurons and extracellular matrix are fundamental for the normal development of the central nervous system. Guidance cues are provided by extracellular factors that orient neuronal migration. During cerebellar development, the extracellular matrix molecules laminin and fibronectin give support to neuronal precursor migration, while other molecules...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Central nervous system; Cerebellum; Neuron; Migration.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300001

Maternal administration of cannabidiol promotes an anti-inflammatory effect on the intestinal wall in a gastroschisis rat model
Provedor de dados: 56
Autores: Callejas,G.H.; Figueira,R.L.; Gonçalves,F.L.L.; Volpe,F.A.P.; Zuardi,A.W.; Crippa,J.A.; Hallak,J.E.; Sbragia,L.
Gastroschisis (GS) is an abdominal wall defect that results in histological and morphological changes leading to intestinal motility perturbation and impaired absorption of nutrients. Due to its anti-inflammatory, antioxidant, and neuroprotective effects, cannabidiol (CBD) has been used as a therapeutic agent in many diseases. Our aim was to test the effect of maternal CBD in the intestine of an experimental model of GS. Pregnant rats were treated over 3 days with CBD (30 mg/kg) after the surgical induction of GS (day 18.5 of gestation) and compared to controls. Fetuses were divided into 4 groups: 1) control (C); 2) C+CBD (CCBD); 3) gastroschisis (G), and 4) G+CBD (GCBD). On day 21.5 of gestation, the fetuses were harvested and evaluated for: a) body...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastroschisis; Experimental model; Prenatal treatment; Cannabidiol.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500607

Modeling pulmonary fibrosis by abnormal expression of telomerase/apoptosis/collagen V in experimental usual interstitial pneumonia
Provedor de dados: 56
Autores: Parra,E.R.; Pincelli,M.S.; Teodoro,W.R.; Velosa,A.P.P.; Martins,V.; Rangel,M.P.; Barbas-Filho,J.V.; Capelozzi,V.L.
Limitations on tissue proliferation capacity determined by telomerase/apoptosis balance have been implicated in pathogenesis of idiopathic pulmonary fibrosis. In addition, collagen V shows promise as an inductor of apoptosis. We evaluated the quantitative relationship between the telomerase/apoptosis index, collagen V synthesis, and epithelial/fibroblast replication in mice exposed to butylated hydroxytoluene (BHT) at high oxygen concentration. Two groups of mice were analyzed: 20 mice received BHT, and 10 control mice received corn oil. Telomerase expression, apoptosis, collagen I, III, and V fibers, and hydroxyproline were evaluated by immunohistochemistry, in situ detection of apoptosis, electron microscopy, immunofluorescence, and histomorphometry....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BHT experimental model; Usual interstitial pneumonia; Alveolar epithelial cell apoptosis; Telomerase activity; Collagen V; Electron microscopy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700567

Cyclosporin-A does not affect skeletal muscle mass during disuse and recovery
Provedor de dados: 56
Autores: Aoki,M.S.; Miyabara,E.H.; Soares,A.G.; Salvini,T.F.; Moriscot,A.S.
Cyclosporin-A (CsA) is an immunosuppressive drug that acts as an inhibitor of calcineurin, a calcium phosphatase that has been suggested to play a role in skeletal muscle hypertrophy. The aim of the present study was to determine the effect of CsA administration (25 mg kg-1 day-1) on skeletal muscle mass and phenotype during disuse and recovery. Male Wistar rats received vehicle (N = 8) or CsA (N = 8) during hind limb immobilization (N = 8) and recovery (N = 8). Muscle weight (dry/wet) and cross-sectional area were evaluated to verify the effect of CsA treatment on muscle mass. Muscle phenotype was assessed by histochemistry of myosin ATPase. CsA administration during immobilization and recovery did not change muscle/body weight ratio in the soleus (SOL)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcineurin; Immobilization; Recovery; Skeletal muscle; Hypertrophy; Cyclosporin-A.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200011

Altered mean platelet volume in patients with polymyositis and its association with disease severity
Provedor de dados: 56
Autores: Peng,Y.-F.; Huang,Y.-X.; Wei,Y.-S.
Polymyositis (PM) is an autoimmune disease characterized by chronic inflammation in skeletal muscle. Mean platelet volume (MPV), a marker in the assessment of systemic inflammation, is easily measured by automatic blood count equipment. However, to our knowledge, there are no data in the literature with respect to MPV levels in PM patients. Therefore, in this study we aimed to investigate MPV levels in patients with PM. This study included 92 newly diagnosed PM patients and 100 healthy individuals. MPV levels were found to be significantly lower compared with healthy controls (10.3±1.23 vs 11.5±0.74 fL, P&lt;0.001). Interestingly, MPV was found to be positively correlated with manual muscle test (MMT) score and negatively correlated with erythrocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mean platelet volume; Polymyositis; Disease severity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600701

Biochemical characterization of the GM2 gangliosidosis B1 variant
Provedor de dados: 56
Autores: Tutor,J.C.
The deficiency of the A isoenzyme of ß-hexosaminidase (Hex) produced by different mutations of the gene that codes for the alpha subunit (Tay-Sachs disease) has two variants with enzymological differences: the B variant consists of the absence of Hex A isoenzyme and the B1 variant produces an inactive Hex A isoenzyme for the hydrolysis of the GM2 ganglioside and synthetic substrates with negative charge. In contrast to the early childhood form of the B variant, the B1 variant appears at a later clinical stage (3 to 7 years of age) with neurodegenerative symptoms leading to the death of the patient in the second decade of life. The most frequent mutation responsible for the GM2 gangliosidosis B1 variant is R178H, which has a widespread geographic and ethnic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GM2 gangliosidosis B1 variant; SS-Hexosaminidase isoenzymes; Activation energy; Biochemical characterization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600001

Evaluation of the antischistosomal activity of sulfated α-D-glucan from the lichen Ramalina celastri free and encapsulated into liposomes
Provedor de dados: 56
Autores: Araújo,R.V.S.; Melo-Júnior,M.R.; Beltrão,E.I.C.; Mello,L.A.; Iacomini,M.; Carneiro-Leão,A.M.A.; Carvalho Jr,L.B.; Santos-Magalhães,N.S.
The antischistosomal activity of the sulfated polysaccharide α-D-glucan (Glu.SO4) extracted from Ramalina celastri was evaluated after encapsulation into liposomes (Glu.SO4-LIPO) in Schistosoma mansoni-infected mice. The effect of treatment with Glu.SO4 and Glu.SO4-LIPO (10 mg/kg) on egg elimination, worm burden and hepatic granuloma formation was assessed using female albino Swiss mice, 35-40 days of age, weighing 25 ± 2 g, infected with 150 cercariae/animal (Biomphalaria glabrata, BH strain). Four groups (N = 10) were studied, two controls (empty liposomes and NaCl) and two treated groups (Glu.SO4-LIPO and Glu.SO4) using a single dose. Parasitological analysis revealed that Glu.SO4-LIPO was as efficient as Glu.SO4 in reducing egg elimination and worm...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polysaccharide; Α-D-glucan; Ramalina celastri; Liposomes; Schistosoma mansoni; Lectin histochemistry.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400007

High-frequency electroacupuncture versus carprofen in an incisional pain model in rats
Provedor de dados: 56
Autores: Teixeira,F.M.; Castro,L.L.; Ferreira,R.T.; Pires,P.A.; Vanderlinde,F.A.; Medeiros,M.A.
The objective of the present study was to compare the effect of electroacupuncture (EA) and carprofen (CP) on postoperative incisional pain using the plantar incision (PI) model in rats. A 1-cm longitudinal incision was made through skin, fascia and muscles of a hind paw of male Wistar rats and the development of mechanical and thermal hypersensitivity was determined over 4 days using the von Frey and Hargreaves methods, respectively. Based on the experimental treatments received on the third postoperative day, the animals were divided into the following groups: PI+CP (CP, 2 mg/kg, po); PI+EAST36 (100-Hz EA applied bilaterally at the Zusanli point (ST36)); PI+EANP (EA applied to a non-acupoint region); PI+IMMO (immobilization only); PI (vehicle). In the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperalgesia; Plantar incision; Acupuncture; Zusanli point; Non-steroidal anti-inflammatory.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200014

Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion
Provedor de dados: 56
Autores: Prezoto,B.C.; Couto,G.K.; Rossoni,L.V.; Schoorlemmer,G.H.M.; Carillo,B.A.; Campos,R.R.
The generation of bradykinin (BK; Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg) in blood and kallidin (Lys-BK) in tissues by the action of the kallikrein-kinin system has received little attention in non-mammalian vertebrates. In mammals, kallidin can be generated by the coronary endothelium and myocytes in response to ischemia, mediating cardioprotective events. The plasma of birds lacks two key components of the kallikrein-kinin system: the low molecular weight kininogen and a prekallikrein activator analogous to mammalian factor XII, but treatment with bovine plasma kallikrein generates ornitho-kinin [Thr6,Leu8]-BK. The possible cardioprotective effect of ornitho-kinin infusion was investigated in an anesthetized, open-chest chicken model of acute coronary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Infarct size; Kallikrein-kinin system; Ornitho-kinin; Factor XII.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900009

L-histidine provokes a state-dependent memory retrieval deficit in mice re-exposed to the elevated plus-maze
Provedor de dados: 56
Autores: Serafim,K.R.; Kishi,M.; Canto-de-Souza,A.; Mattioli,R.
The effects of L-histidine (LH) on anxiety and memory retrieval were investigated in adult male Swiss Albino mice (weight 30-35 g) using the elevated plus-maze. The test was performed on two consecutive days: trial 1 (T1) and trial 2 (T2). In T1, mice received an intraperitoneal injection of saline (SAL) or LH before the test and were then injected again and retested 24 h later. LH had no effect on anxiety at the dose of 200 mg/kg since there was no difference between the SAL-SAL and LH-LH groups at T1 regarding open-arm entries (OAE) and open-arm time (OAT) (mean ± SEM; OAE: 4.0 ± 0.71, 4.80 ± 1.05; OAT: 40.55 ± 9.90, 51.55 ± 12.10, respectively; P &gt; 0.05, Kruskal-Wallis test), or at the dose of 500 mg/kg (OAE: 5.27 ± 0.73, 4.87 ± 0.66; OAT: 63.93 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-histidine; Anxiety; Emotional memory; Elevated plus-maze; Memory retrieval deficit.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100014

Cirrhotic cardiomyopathy: the liver affects the heart
Provedor de dados: 56
Autores: Carvalho,M.V.H.; Kroll,P.C.; Kroll,R.T.M.; Carvalho,V.N.
Cirrhotic cardiomyopathy historically has been confused as alcoholic cardiomyopathy. The key points for diagnosis of cirrhotic cardiomyopathy have been well explained, however this entity was neglected for a long time. Nowadays the diagnosis of this entity has become important because it is a factor that contributes significantly to morbidity-mortality in cirrhotic patients. Characteristics of cirrhotic cardiomyopathy are a hyperdynamic circulatory state, altered diastolic relaxation, impaired contractility, and electrophysiological abnormalities, particularity QT interval prolongation. The pathogenesis includes impaired function of beta-receptors, altered transmembrane currents and overproduction of cardiodepressant factors, such as nitric oxide,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cirrhotic cardiomyopathy; Cirrhosis; Heart failure; Cardiac cirrhosis; Hyperdynamic circulation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200302

The starch from Solanum lycocarpum St. Hill. fruit is not a hypoglycemic agent
Provedor de dados: 56
Autores: Oliveira,A.C.P.; Endringer,D.C.; Araújo,R.J.P.; Brandão,M.G.L.; Coelho,M.M.
We have investigated the hypoglycemic effect induced by the starch obtained from the unripe fruits of Solanum lycocarpum (Solanaceae). Per os administration of the starch (1000 or 2000 mg/kg, twice daily for 7 days, N = 6) did not change glycemia levels of nondiabetic female Swiss mice weighing 25-30 g. In streptozotocin-induced diabetic mice, similar treatment with the starch did not change the elevated glycemia 3 h after the last dose (diabetic treated with saline = 288 ± 17/309 ± 18; starch 1000 mg/kg = 295 ± 33; starch 2000 mg/kg = 258 ± 37; N = 5). In animals fasted for 15 h, per os administration of glucose (600 mg/kg) significantly increased glycemia 1 h later. Previous (-30 min) treatment of the animals with the starch (1000 or 2000 mg/kg; N = 5)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Solanum lycocarpum St. Hill.; Glycoalkaloids; Starch fraction; Diabetes mellitus; Hypoglycemic effect.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400017

Linear and nonlinear analysis of heart rate variability in healthy subjects and after acute myocardial infarction in patients
Provedor de dados: 56
Autores: Kunz,V.C.; Borges,E.N.; Coelho,R.C.; Gubolino,L.A.; Martins,L.E.B.; Silva,E.
The objectives of this study were to evaluate and compare the use of linear and nonlinear methods for analysis of heart rate variability (HRV) in healthy subjects and in patients after acute myocardial infarction (AMI). Heart rate (HR) was recorded for 15 min in the supine position in 10 patients with AMI taking β-blockers (aged 57 ± 9 years) and in 11 healthy subjects (aged 53 ± 4 years). HRV was analyzed in the time domain (RMSSD and RMSM), the frequency domain using low- and high-frequency bands in normalized units (nu; LFnu and HFnu) and the LF/HF ratio and approximate entropy (ApEn) were determined. There was a correlation (P &lt; 0.05) of RMSSD, RMSM, LFnu, HFnu, and the LF/HF ratio index with the ApEn of the AMI group on the 2nd (r = 0.87, 0.65,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial infarction; Heart rate variability; Autonomic nervous system; Spectral analysis; Nonlinear dynamics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500011

Ghrelin plasma levels, gastric ghrelin cell density and bone mineral density in women with rheumatoid arthritis
Provedor de dados: 56
Autores: Maksud,F.A.N.; Kakehasi,A.M.; Guimarães,M.F.B.R.; Machado,C.J.; Barbosa,A.J.A.
Generalized bone loss can be considered an extra-articular manifestation of rheumatoid arthritis (RA) that may lead to the occurrence of fractures, resulting in decreased quality of life and increased healthcare costs. The peptide ghrelin has demonstrated to positively affect osteoblasts in vitro and has anti-inflammatory actions, but the studies that correlate ghrelin plasma levels and RA have contradictory results. We aimed to evaluate the correlation between total ghrelin plasma levels, density of ghrelin-immunoreactive cells in the gastric mucosa, and bone mineral density (BMD) in twenty adult women with established RA with 6 months or more of symptoms (mean age of 52.70±11.40 years). Patients with RA presented higher ghrelin-immunoreactive cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ghrelin; Gastric mucosa; Bone metabolism; Osteoporosis; Rheumatoid arthritis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600704

Oxidation of the albumin thiol to sulfenic acid and its implications in the intravascular compartment
Provedor de dados: 56
Autores: Turell,L.; Carballal,S.; Botti,H.; Radi,R.; Alvarez,B.
Human serum albumin (HSA) is the most abundant protein in the intravascular compartment. It possesses a single thiol, Cys34, which constitutes ~80% of the total thiols in plasma. This thiol is able to scavenge plasma oxidants. A central intermediate in this potential antioxidant activity of human serum albumin is sulfenic acid (HSA-SOH). Work from our laboratories has demonstrated the formation of a relatively stable sulfenic acid in albumin through complementary spectrophotometric and mass spectrometric approaches. Recently, we have been able to obtain quantitative data that allowed us to measure the rate constants of sulfenic acid reactions with molecules of analytical and biological interest. Kinetic considerations led us to conclude that the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thiol; Sulfhydryl; Albumin; Sulfenic acid; Mixed disulfides; Plasma.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400001

Neuroendocrine control of body fluid homeostasis
Provedor de dados: 56
Autores: McCann,S.M.; Gutkowska,J.; Antunes-Rodrigues,J.
Angiotensin II and atrial natriuretic peptide (ANP) play important and opposite roles in the control of water and salt intake, with angiotensin II promoting the intake of both and ANP inhibiting the intake of both. Following blood volume expansion, baroreceptor input to the brainstem induces the release of ANP within the hypothalamus that releases oxytocin (OT) that acts on its receptors in the heart to cause the release of ANP. ANP activates guanylyl cyclase that converts guanosine triphosphate into cyclic guanosine monophosphate (cGMP). cGMP activates protein kinase G that reduces heart rate and force of contraction, decreasing cardiac output. ANP acts similarly to induce vasodilation. The intrinsic OT system in the heart and vascular system augments the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxytocin; Vasopressin; Atrial natriuretic peptide; Nitric oxide; Angiotensin II; Guanylyl cyclase.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200003

Effects of sirolimus alone or in combination with cyclosporine A on renal ischemia/reperfusion injury
Provedor de dados: 56
Autores: Pereira,B.J.; Castro,I.; Burdmann,E.A.; Malheiros,D.M.A.; Yu,L.
Calcineurin inhibitors exacerbate ischemic injury in transplanted kidneys, but it is not known if sirolimus protects or exacerbates the transplanted kidney from ischemic injury. We determined the effects of sirolimus alone or in combination with cyclosporin A (CsA) on oxygenated and hypoxic/reoxygenated rat proximal tubules in the following in vitro groups containing 6-9 rats per group: sirolimus (10, 50, 100, 250, 500, and 1000 ηg/mL); CsA (100 µg/mL); sirolimus (50 and 250 ηg/mL) + CsA (100 µg/mL); control; vehicle (20% ethanol). For in vivo studies, 3-week-old Wistar rats (150-250 g) were submitted to left nephrectomy and 30-min renal artery clamping. Renal function and histological evaluation were performed 24 h and 7 days after ischemia (I) in five...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclosporin A; Nephrotoxicity; Renal proximal tubules; Renal ischemia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800007

Minor sperm abnormalities in young male post-pubertal patients with juvenile dermatomyositis
Provedor de dados: 56
Autores: Moraes,A.J.P.; Pereira,R.M.R.; Cocuzza,M.; Casemiro,R.; Saito,O.; Silva,C.A.A.
The objective of the present study was to identify sperm abnormalities in young male patients with juvenile dermatomyositis (JDM). In 2005, 18 male JDM patients, diagnosed according to the criteria of Bohan and Peter, were followed at the Pediatric Rheumatology Unit and Rheumatology Division, of our Institution. Of the 18 males, 11 were pre-pubertal and 7 were post-pubertal. Two of 7 post-pubertal JDM male patients were excluded: one for orchidopexy for cryptorchidism and the other for testicular ectopia in the left testis. The remaining 5 post-pubertal JDM patients were prospectively evaluated on the basis of two semen analyses, according to the World Health Organization (WHO), urologic evaluation, testicular Doppler ultrasound hormone profile. The data...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Juvenile dermatomyositis; Gonad; Sperm; Hormone; Male post-puberty; Idiopathic inflammatory myopathy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200016

Interferon gamma is a key cytokine in lung phase immunity to schistosomes but what is its precise role?
Provedor de dados: 56
Autores: Wilson,R.A.
Vaccination of mice with radiation-attenuated cercariae of Schistosoma mansoni induces a high level of protection against challenge with normal larvae. The immune effector mechanism, which operates in the lungs, is a cell-mediated delayed-type hypersensitivity response and involves the formation of a tight focus of mononuclear cells around embolised larvae. CD4+ T cells with Th1 characteristics are a major component of the infiltrate. They secrete abundant interferon gamma (IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;) upon antigen stimulation in vitro, whilst in vivo neutralisation of the cytokine results in 90% abrogation of immunity. IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; can induce a large number of genes and an attempt has been made to identify...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interferon gamma; Schistosoma mansoni; Lung phase immunity; Nitric oxide; Adhesion molecule.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100022

Imbalance of naive and memory T lymphocytes with sustained high cellular activation during the first year of life from uninfected children born to HIV-1-infected mothers on HAART
Provedor de dados: 56
Autores: Ono,E.; Santos,A.M. Nunes dos; Succi,R.C. de Menezes; Machado,D.M.; Angelis,D.S.A. de; Salomão,R.; Kallás,E.G.; Moraes-Pinto,M.I. de.
The immune consequences of in utero HIV exposure to uninfected children whose mothers were submitted to highly active antiretroviral therapy (HAART) during gestation are not well defined. We evaluated 45 HIV-exposed uninfected (ENI) neonates and 45 healthy unexposed control (CT) neonates. All HIV-infected mothers received HAART during pregnancy, and the viral load at delivery was &lt;50 copies/mL for 56.8%. Twenty-three ENI neonates were further evaluated after 12 months and compared to 23 unexposed healthy age-matched infants. Immunophenotyping was performed by flow cytometry in cord and peripheral blood. Cord blood lymphocyte numbers did not differ between groups. However, ENI neonates had a lower percentage of naive T cells than CT neonates (CD4+, 76.6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunophenotyping; Highly active antiretroviral therapy; HIV-1; Immunological stimulation; Neonates of HIV-infected mothers.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800011

Modifications of the sympathetic skin response in workers chronically exposed to lead
Provedor de dados: 56
Autores: Nora,D.B.; Gomes,I.; Said,G.; Carvalho,F.M.; Melo,A.
The long-term effects of low-level lead intoxication are not known. The sympathetic skin response (SSR) was evaluated in a group of 60 former workers of a primary lead smelter, located in Santo Amaro, BA, Brazil. The individuals participating in the study were submitted to a clinical-epidemiological evaluation including questions related to potential risk factors for intoxication, complaints related to peripheral nervous system (PNS) involvement, neurological clinical examination, and also to electromyography and nerve conduction studies and SSR evaluation. The sample consisted of 57 men and 3 women aged 34 to 69 years (mean ± SD: 46.8 ± 6.9). The neurophysiologic evaluation showed the presence of lumbosacral radiculopathy in one of the individuals (1.7%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuropathy; Sympathetic skin response; Lead intoxication; Peripheral nervous system.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100011

Evolution of circadian organization in vertebrates
Provedor de dados: 56
Circadian organization means the way in which the entire circadian system above the cellular level is put together physically and the principles and rules that determine the interactions among its component parts which produce overt rhythms of physiology and behavior. Understanding this organization and its evolution is of practical importance as well as of basic interest. The first major problem that we face is the difficulty of making sense of the apparently great diversity that we observe in circadian organization of diverse vertebrates. Some of this diversity falls neatly into place along phylogenetic lines leading to firm generalizations: i) in all vertebrates there is a "circadian axis" consisting of the retinas, the pineal gland and the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Circadian rhythms; Evolution; Vertebrates; Pineal; SCN; Retina; Melatonin.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300003

Reliability and validity of a Portuguese version of the Young Mania Rating Scale
Provedor de dados: 56
Autores: Vilela,J.A.A.; Crippa,J.A.S.; Del-Ben,C.M.; Loureiro,S.R.
The reliability and validity of a Portuguese version of the Young Mania Rating Scale were evaluated. The original scale was translated into and adapted to Portuguese by the authors. Definitions of clinical manifestations, a semi-structured anchored interview and more explicit rating criteria were added to the scale. Fifty-five adult subjects, aged 18 to 60 years, with a diagnosis of Current Manic Episode according to DSM-III-R criteria were assessed using the Young Mania Rating Scale as well as the Brief Psychiatric Rating Scale in two sessions held at intervals from 7 to 10 days. Good reliability ratings were obtained, with intra-class correlation coefficient of 0.97 for total scores, and levels of agreement above 0.80 (P &lt; 0.001) for all individual...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mania; Rating scales; Reliability; Validity; Brief Psychiatric Rating Scale.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900019

Effects of sodium valproate and carbamazepine on food competition aggression in pigeons
Provedor de dados: 56
Autores: Fachinelli,C.; Ahumada,M.; Fachinellizz,J.M.; Torrecilla,M.; Rodríguez-Echandía,E.L.
Valproate and carbamazepine (CAR) have been proposed as adjunct alternatives for the control of aggression in psychiatric patients, although no definite conclusions have been reached. We examined the effects of these drugs on food competition offensive aggression and other behaviors in high- and low-aggression food-restricted pigeons. These were divided into pairs containing previously ranked high-aggression (N = 10 pairs) and low-aggression females (N = 10 pairs). In Experiment 1, a pigeon in each pair of high- and low-aggression subjects was treated daily with an oral dose of sodium valproate (50 mg kg-1 mL saline-1) for 15 days. The other animal received the vehicle. On days 1, 7, and 15, food competition trials (10 min) were performed 60 min after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Valproate; Carbamazepine; Aggressive behavior; Food competition; Pigeons.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600015

Effects of heme oxygenase-1 recombinant Lactococcus lactis on the intestinal barrier of hemorrhagic shock rats
Provedor de dados: 56
Autores: Gao,X.Y.; Zhou,X.F.; Wang,H.; Lv,N.; Liu,Y.; Guo,J.R.
This study aimed to investigate the effects of heme oxygenase-1 recombinant Lactococcus lactis (LL-HO-1) on the intestinal barrier of rats with hemorrhagic shock. One hundred Sprague-Dawley male rats (280–320 g) were randomly divided into healthy control group (N group) and hemorrhagic shock group (H group). Each group was subdivided into HO1t, HO2t, HO3t, PBS and LL groups in which rats were intragastrically injected with LL-HO-1 once, twice and three times, PBS and L. lactis (LL), respectively. The mortality, intestinal myeloperoxidase (MPO) activity, intestinal contents of TNF-α, IL-10 and HO-1, and intestinal Chiu's score were determined. Results showed that in N group, the HO-1 content increased after LL-HO-1 treatment, and significant difference was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heme oxygenase-1; Hemorrhagic shock; Lactococcus lactis; Intestinal barrier; Intestinal inflammation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700601

Lateral asymmetry of voluntary attention orienting
Provedor de dados: 56
Autores: Castro-Barros,B.A.; Lacerda,A.M.; Righi,L.L.; Ribeiro-do-Valle,L.E.
We recently demonstrated that automatic attention favors the right side of space and, in the present study, we investigated whether voluntary attention also favors this side. Six reaction time experiments were conducted. In each experiment, 12 new 18-25-year-old male right-handed individuals were tested. In Experiments 1, 2, 3 (a, b) and 4 (a, b), tasks with increasing attentional demands were used. In Experiments 1, 2, 3a, and 4a, attention was oriented to one or both sides by means of a central spatially informative visual cue. A left or right side visual target appeared 100, 300, or 500 ms later. Attentional effects were observed in the four experiments. In Experiments 2, 3a and 4a, these effects were greater when the cue indicated the right side than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Voluntary attention; Visuospatial attention; Lateral asymmetry; Reaction time tasks.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800008

Improvement of the indirect hemagglutination test for the detection of antibodies to Streptococcus pyogenes
Provedor de dados: 56
Autores: Rubinsky-Elefant,G.; Hoshino-Shimizu,S.; Mamizuka,E.M.; Asciutti,M.M.R.
An indirect hemagglutination test for a seroepidemiological survey of Streptococcus pyogenes infection was standardized. This is an improved modification of the indirect hemagglutination test which utilizes an unstable reagent prepared with fresh blood cells. Two types of bacterial antigens represented by extracellular products and purified streptolysin O were assayed, but only the former antigen gave good results. Pretreatment of the bacterial antigen with 0.15 M NaOH and neutralization to pH 5.5, as well as postfixation of sensitized red cells with 0.1% glutaraldehyde at 56oC for 30 min were found to be essential to give long stability to the reagent in liquid suspension, at least 9 months at 4oC. A total of 564 serum samples with high, moderate and low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pyogenes; Hemagglutination test; Anti-streptolysin O antibodies.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800008

Comparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the ProCount™-BD and ISHAGE protocols
Provedor de dados: 56
Autores: Pranke,P.; Hendrikx,J.; Alespeiti,G.; Nardi,N.; Rubinstein,P.; Visser,J.
The total number of CD34+ cells is the most relevant clinical parameter when selecting human umbilical cord blood (HUCB) for transplantation. The objective of the present study was to compare the two most commonly used CD34+ cell quantification methods (ISHAGE protocol and ProCount™ - BD) and analyze the CD34+ bright cells whose 7-amino actinomycin D (7AAD) analysis suggests are apoptotic or dead cells. Twenty-six HUCB samples obtained at the Placental Blood Program of New York Blood Center were evaluated. The absolute numbers of CD34+ cells evaluated by the ISHAGE (with exclusion of 7AAD+ cells) and ProCount™ (with exclusion of CD34+ bright cells) were determined. Using the ISHAGE protocol we found 35.6 ± 19.4 CD34+ cells/µL and with the ProCount™ method...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematopoietic stem cell; Human umbilical cord blood; CD34+; ISHAGE; ProCount.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700008

A majority of Brazilian patients with rheumatoid arthritis HLA-DRB1 alleles carry both the HLA-DRB1 shared epitope and anti-citrunillated peptide antibodies
Provedor de dados: 56
Autores: Louzada-Júnior,P.; Freitas,M.V.C.; Oliveira,R.D.R.; Deghaide,N.H.S.; Conde,R.A.; Bertolo,M.B.; Donadi,E.A.
The objective of the present study was to evaluate the contribution of the shared epitope (SE), the rheumatoid arthritis (RA) protection model, and the occurrence of anti-cyclic citrullinated peptide (anti-CCP) antibodies in RA patients from a genetically diverse population. One hundred and forty Brazilian RA patients and 161 matched controls were typed for HLA-DRB1 alleles using amplified DNA hybridized with sequence-specific oligonucleotide probes or primers. Patients were stratified according to the presence or absence of SE (DRB1*0401, *0404, *0405, *0101, *1001, and *1402), of the DERAA alleles (DRB1*0103, *0402, *1102, *1103, *1301, *1302, and *1304), and X (all other alleles). Anti-CCP antibodies were measured by ELISA. The combined frequency of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Susceptibility to rheumatoid arthritis; HLA; Brazilians; Shared epitope; Anti-cyclic citrullinated peptide.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600010

Evidence for eosinophil recruitment, leukotriene B4 production and mast cell hyperplasia following Toxocara canis infection in rats
Provedor de dados: 56
Autores: Carlos,D.; Machado,E.R.; De Paula,L.; Sá-Nunes,A.; Sorgi,C.A.; Jamur,M.C.; Oliver,C.; Lima,W.T.; Faccioli,L.H.
It is well known that eosinophilia is a key pathogenetic component of toxocariasis. The objective of the present study was to determine if there is an association between peritoneal and blood eosinophil influx, mast cell hyperplasia and leukotriene B4 (LTB4) production after Toxocara canis infection. Oral inoculation of 56-day-old Wistar rats (N = 5-7 per group) with 1000 embryonated eggs containing third-stage (L3) T. canis larvae led to a robust accumulation of total leukocytes in blood beginning on day 3 and peaking on day 18, mainly characterized by eosinophils and accompanied by higher serum LTB4 levels. At that time, we also noted increased eosinophil numbers in the peritoneal cavity. In addition, we observed increased peritoneal mast cell number in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mast cells; Leukotriene B4; Eosinophils; Eosinophil peroxidase; Toxocara canis; Rat.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400008

Classification of brain tumor extracts by high resolution ¹H MRS using partial least squares discriminant analysis
Provedor de dados: 56
Autores: Faria,A.V.; Macedo Jr.,F.C.; Marsaioli,A.J.; Ferreira,M.M.C.; Cendes,F.
High resolution proton nuclear magnetic resonance spectroscopy (¹H MRS) can be used to detect biochemical changes in vitro caused by distinct pathologies. It can reveal distinct metabolic profiles of brain tumors although the accurate analysis and classification of different spectra remains a challenge. In this study, the pattern recognition method partial least squares discriminant analysis (PLS-DA) was used to classify 11.7 T ¹H MRS spectra of brain tissue extracts from patients with brain tumors into four classes (high-grade neuroglial, low-grade neuroglial, non-neuroglial, and metastasis) and a group of control brain tissue. PLS-DA revealed 9 metabolites as the most important in group differentiation: γ-aminobutyric acid, acetoacetate, alanine,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain; Tumor; Magnetic resonance spectroscopy; Spectroscopy; Metabolism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200009

Profile of the Brazilian scientific production in multiple sclerosis
Provedor de dados: 56
Autores: Araujo,C.R.; Moreira,M.A.; Lana-Peixoto,M.A.
This paper analyzes the profile of the Brazilian output in the field of multiple sclerosis from 1981 to 2004. The search was conducted through the MEDLINE and LILACS databases, selecting papers in which the term "multiple sclerosis" was defined as the main topic and "Brazil" or "Brasil" as others. The data were analyzed regarding the themes, the state in Brazil and institution where the papers were produced, the journals where the papers were published, journal's impact factor, and language. The search disclosed 141 documents (91 from MEDLINE and LILACS, and 50 from LILACS only) published in 44 different journals (23 of them MEDLINE-indexed). A total of 111 documents were produced by 17 public universities, 29 by 3 private medical schools and 1 by a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Multiple sclerosis; Scientific production; Brazil.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900001

Fatty acid composition of vegetable oil blend and in vitro effects of pharmacotherapeutical skin care applications
Provedor de dados: 56
Autores: Guidoni,M.; de Christo Scherer,M.M.; Figueira,M.M.; Schmitt,E.F.P.; de Almeida,L.C.; Scherer,R.; Bogusz,S.; Fronza,M.
Vegetable oils have been used for a plethora of health benefits by their incorporation in foods, cosmetics, and pharmaceutical products, especially those intended for skin care. This study aimed to investigate the cutaneous benefits of a vegetable oil blend (VOB) formulation and its fatty acid composition. The anti-inflammatory activity was studied in macrophages of RAW 264.7 cells by investigating the release of nitric oxide (NO), superoxide anion generation (O2-), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6). ABTS cation radical scavenging capacity assay, ferric reducing antioxidant potential (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and NO free radical scavenging assays were used to evaluate the antioxidant activity. VOB was tested...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vegetable oil blend; Skin care; Fatty acids; Anti-inflammatory; Nitric oxide; Scratch assay.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200609

Relative influence of age, resting heart rate and sedentary life style in short-term analysis of heart rate variability
Provedor de dados: 56
Autores: Migliaro,E.R.; Contreras,P.; Bech,S.; Etxagibel,A.; Castro,M.; Ricca,R.; Vicente,K.
In order to assess the relative influence of age, resting heart rate (HR) and sedentary life style, heart rate variability (HRV) was studied in two different groups. The young group (YG) consisted of 9 sedentary subjects aged 15 to 20 years (YG-S) and of 9 nonsedentary volunteers (YG-NS) also aged 15 to 20. The elderly sedentary group (ESG) consisted of 16 sedentary subjects aged 39 to 82 years. HRV was assessed using a short-term procedure (5 min). R-R variability was calculated in the time-domain by means of the root mean square successive differences. Frequency-domain HRV was evaluated by power spectrum analysis considering high frequency and low frequency bands. In the YG the effort tolerance was ranked in a bicycle stress test. HR was similar for both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart rate variability; Short-term analysis; Autonomic nervous system; Aging; Sedentary life style.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400009

Differences between remaining ability and loss of capacity in maximum aerobic impairment
Provedor de dados: 56
Autores: Neder,J.A.; Nery,L.E.; Bagatin,E.; Lucas,S.R.; Anção,M.S.; Sue,D.Y.
In the evaluation of exercise intolerance of patients with respiratory diseases the American Medical Association (AMA) and the American Thoracic Society (ATS) have proposed similar classifications for rating aerobic impairment using maximum oxygen uptake (VO2max) normalized for total body weight (ml min-1 kg-1). However, subjects with the same VO2max weight-corrected values may have considerably different losses of aerobic performance (VO2max expressed as % predicted). We have proposed a new, specific method for rating loss of aerobic capacity (VO2max, % predicted) and we have compared the two classifications in a prospective study involving 75 silicotic claimants. Logistic regression analysis showed that the disagreement between rating systems (higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Disability evaluation; Work capacity; Exercise tolerance; Oxygen consumption; Occupational diseases.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500006

Interrelationship between serum and sputum inflammatory mediators in chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Bizeto,L.; Mazzolini,A.B.; Ribeiro,M.; Stelmach,R.; Cukier,A.; Nunes,M.P.T.
Little is known about airway inflammatory markers in chronic obstructive pulmonary disease (COPD). The objective of the present study was to identify and try to correlate pulmonary and peripheral blood inflammatory markers in COPD. In a cross-sectional study on patients with stable COPD, induced sputum and blood samples were collected for the determination of C-reactive protein, eosinophilic cationic protein, serum amyloid A protein, a-1 antitrypsin (a-1AT), and neutrophil elastase. Twenty-two patients were divided into two groups according to post-bronchodilator forced expiratory volume in the first second (%FEV1): group 1 (N = 12, FEV1 &lt;40%) and group 2 (N = 10, FEV1 ³40%). An increase in serum elastase, eosinophilic cationic protein and a-1AT was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic obstructive pulmonary disease; C-reactive protein; Eosinophilic cationic protein; Serum amyloid A protein; A-1 antitrypsin; Neutrophil elastase.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300003

Category fluency test: effects of age, gender and education on total scores, clustering and switching in Brazilian Portuguese-speaking subjects
Provedor de dados: 56
Autores: Brucki,S.M.D.; Rocha,M.S.G.
Verbal fluency tests are used as a measure of executive functions and language, and can also be used to evaluate semantic memory. We analyzed the influence of education, gender and age on scores in a verbal fluency test using the animal category, and on number of categories, clustering and switching. We examined 257 healthy participants (152 females and 105 males) with a mean age of 49.42 years (SD = 15.75) and having a mean educational level of 5.58 (SD = 4.25) years. We asked them to name as many animals as they could. Analysis of variance was performed to determine the effect of demographic variables. No significant effect of gender was observed for any of the measures. However, age seemed to influence the number of category changes, as expected for a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Verbal fluency; Educational level; Neuropsychological tests; Category fluency.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200002

Age-related changes in radiation-induced micronuclei among healthy adults
Provedor de dados: 56
Autores: Joksic,G.; Petrovic,S.; Ilic,Z.
The aim of the present study was to establish the extent of in vitro radioresponse of lymphocytes among 62 healthy adults of both genders and to estimate the distribution of baseline micronuclei and radiosensitivity among individuals of the study population using the cytochalasin block micronucleus test. A younger study group consisted of 10 males (mean age, 22.4 years; range, 21-27) and 12 females (mean age, 24.8 years; range, 20-29), whereas an older study group consisted of 18 males (mean age, 35.1 years; range, 30-44) and 22 females (mean age, 38.5 years; range, 30-48). For evaluation of radiosensitivity blood samples were irradiated in vitro using 60Co g-ray source. The radiation dose employed was 2 Gy, the dose rate 0.45 Gy/min. The study revealed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human lymphocytes; Baseline micronuclei; Radiosensitivity; Ageing.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800002

Photodynamic therapy: a new concept in medical treatment
Provedor de dados: 56
Autores: Sibata,C.H.; Colussi,V.C.; Oleinick,N.L.; Kinsella,T.J.
A new concept in the therapy of both neoplastic and non-neoplastic diseases is discussed in this article. Photodynamic therapy (PDT) involves light activation, in the presence of molecular oxygen, of certain dyes that are taken up by the target tissue. These dyes are termed photosensitizers. The mechanism of interaction of the photosensitizers and light is discussed, along with the effects produced in the target tissue. The present status of clinical PDT is discussed along with the newer photosensitizers being used and their clinical roles. Despite the promising results from earlier clinical trials of PDT, considerable additional work is needed to bring this new modality of treatment into modern clinical practice. Improvements in the area of light source...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic therapy (PDT); Light delivery; Clinical PDT; State of art of PDT.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800002

Studies of the small bowel surface by scanning electron microscopy in infants with persistent diarrhea
Provedor de dados: 56
Autores: Fagundes-Neto,U.; De Martini-Costa,S.; Pedroso,M.Z.; Scaletsky,I.C.A.
We describe the ultrastructural abnormalities of the small bowel surface in 16 infants with persistent diarrhea. The age range of the patients was 2 to 10 months, mean 4.8 months. All patients had diarrhea lasting 14 or more days. Bacterial overgrowth of the colonic microflora in the jejunal secretion, at concentrations above 10(4) colonies/ml, was present in 11 (68.7%) patients. The stool culture was positive for an enteropathogenic agent in 8 (50.0%) patients: for EPEC O111 in 2, EPEC O119 in 1, EAEC in 1, and Shigella flexneri in 1; mixed infections due to EPEC O111 and EAEC in 1 patient, EPEC O119 and EAEC in 1 and EPEC O55, EPEC O111, EAEC and Shigella sonnei in 1. Morphological abnormalities in the small bowel mucosa were observed in all 16 patients,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Persistent diarrhea; Small bowel; Scanning electron microscopy; Bacterial proliferation; Infants.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200006

Impact of β-2 Thr164Ile and combined β-adrenergic receptor polymorphisms on prognosis in a cohort of heart failure outpatients
Provedor de dados: 56
Autores: Biolo,A.; Salvaro,R.; Clausell,N.; Silvello,D.; Santos,K.G.; Rohde,L.E.
Genetic polymorphisms of adrenergic receptors (ARs) have been associated with the development, progression, and prognosis of patients with heart failure (HF), with few data for the Brazilian population. We evaluated the role of the β2-AR Thr164Ile polymorphism at codon 164 on prognosis in a prospective study on 315 adult Brazilian HF patients, predominantly middle-aged Caucasian men in functional class I-II, with severe left ventricular systolic dysfunction. Genomic DNA was extracted from peripheral blood and β2-AR164 genotypes were detected by PCR followed by restriction fragment length analysis. During a median follow-up of 3 years, 95 deaths occurred and 57 (60%) were HF-related. Unexpectedly, Ile164 carriers (N = 12) had no HF-related events (log-rank...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Adrenergic receptors; Genetic polymorphisms; Prognosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600007

Estrogen and progesterone promote breast cancer cell proliferation by inducing cyclin G1 expression
Provedor de dados: 56
Autores: Tian,J-M.; Ran,B.; Zhang,C-L.; Yan,D-M.; LI,X-H.
Breast cancer is the most common cause of cancer among women in most countries (WHO). Ovarian hormone disorder is thought to be associated with breast tumorigenesis. The present study investigated the effects of estrogen and progesterone administration on cell proliferation and underlying mechanisms in breast cancer MCF-7 cells. It was found that a single administration of estradiol (E2) or progesterone increased MCF-7 cell viability in a dose-dependent manner and promoted cell cycle progression by increasing the percentage of cells in the G2/M phase. A combination of E2 and progesterone led to a stronger effect than single treatment. Moreover, cyclin G1 was up-regulated by E2 and/or progesterone in MCF-7 cells. After knockdown of cyclin G1 in MCF-7 cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Estrogen; Progesterone; Cyclin G1; Cell proliferation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300611

The role of IL-12 in experimental Trypanosoma cruzi infection
Provedor de dados: 56
Autores: Silva,J.S.; Aliberti,J.C.S.; Martins,G.A.; Souza,M.A.; Souto,J.T.; Pádua,M.A.
Host resistance to Trypanosoma cruzi infection is dependent on both natural and acquired immune responses. During the early acute phase of infection in mice, natural killer (NK) cell-derived IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; is involved in controlling intracellular parasite replication, mainly through the induction of nitric oxide biosynthesis by activated macrophages. We have shown that IL-12, a powerful inducer of IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; production by NK cells, is synthesized soon after trypomastigote-macrophage interaction. The role of IL-12 in the control of T. cruzi infection in vivo was determined by treating infected mice with anti-IL-12 monoclonal antibody (mAb) and analyzing both parasitemia and mortality during the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IL-12 Trypanosoma cruzi NK cells IFN-&lt; FONT FACE=Symbol&gt; G&lt; /font&gt; Nitric oxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100014

Possible roles of COX-1 in learning and memory impairment induced by traumatic brain injury in mice
Provedor de dados: 56
Autores: Shang,J.L.; Cheng,Q.; Yang,W.F.; Zhang,M.; Cui,Y.; Wang,Y.F.
People who suffer from traumatic brain injury (TBI) often experience cognitive deficits in spatial reference and working memory. The possible roles of cyclooxygenase-1 (COX-1) in learning and memory impairment in mice with TBI are far from well known. Adult mice subjected to TBI were treated with the COX-1 selective inhibitor SC560. Performance in the open field and on the beam walk was then used to assess motor and behavioral function 1, 3, 7, 14, and 21 days following injury. Acquisition of spatial learning and memory retention was assessed using the Morris water maze on day 15 post-TBI. The expressions of COX-1, prostaglandin E2 (PGE2), interleukin (IL)-6, brain-derived neurotrophic factor (BDNF), platelet-derived growth factor BB (PDGF-BB), synapsin-I,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Traumatic brain injury; COX-1; Learning and memory; SC560.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201050

Wine, alcohol and atherosclerosis: clinical evidences and mechanisms
Provedor de dados: 56
Autores: Luz,P.L. da; Coimbra,S.R.
Atherosclerosis is a chronic inflammatory disease which may cause obstructions of the coronary, cerebral and peripheral arteries. It is typically multifactorial, most often dependent on risk factors such as hypercholesterolemia, diabetes, smoking, hypertension, sedentarism, and obesity. It is the single main cause of death in most developed countries due to myocardial infarction, angina, sudden death, and heart failure. Several epidemiological studies suggest that moderate alcohol intake, especially red wine, decrease cardiac mortality due to atherosclerosis. The alcohol effect is described by a J curve, suggesting that moderate drinkers may benefit while abstainers and heavy drinkers are at higher risk. Experimental studies indicate that most beneficial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary disease; Beverages; Drinks and cardiac protection; Flavonoids; Wine and grape juice.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900001

Seroprevalence of bactericidal antibodies against serogroup B and C Meningococci in a University Hospital
Provedor de dados: 56
Autores: Gioia,C.A.C.; Lemos,A.P.S.; Gorla,M.C.O.; Mendoza-Sassi,R.; Figueredo,B.S.; Ballester,T.; Von Groll,A.; Wedig,B.; Ethur,N.V.; Bragança,L.; Silva,P.E.A.; Milagres,L.G.
Meningococcus serogroup B (MenB), clonal complex 32 (cc 32), was the Brazilian epidemic strain of meningococcal disease (MD) in the 1990’s. Currently, meningococcus serogroup C (MenC), cc 103, is responsible for most of the cases of the disease in Brazil. The aim of this study was to investigate the seroprevalence of bactericidal antibody (SBA) against representative epidemic strains of MenC, (N753/00 strain, C:23:P1.22,14-6, cc103) and MenB, (Cu385/83 strain, B:4,7:P1.15,19, cc32) in students and employees of a university hospital in the State of Rio Grande do Sul (RS, Brazil). A second MenC strain (N79/96, C:2b:P1.5-2,10, cc 8) was used as a prototype strain of Rio de Janeiro’s outbreak that occurred in the 1990’s. Our previous study showed a 9% rate of...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Neisseria meningitidis; Bactericidal antibodies; Meningococcal carrier.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500607

NLRP3 inflammasome signaling as an early molecular response is negatively controlled by miR-186 in CFA-induced prosopalgia mice
Provedor de dados: 56
Autores: Chen,Ming-lei; Lin,Kang; Lin,Shu-kai.
The NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome is the most frequently studied in the central nervous system and has been linked to neuropathic pain. In this study, a post-translational mechanism of microRNA (miR)-186 via regulating the expression of NLRP3 in the complete Freund's adjuvant (CFA)-treated mice was investigated. The injection of CFA was used to induce trigeminal neuropathic pain in mice. miRs microarray chip assay was performed in trigeminal ganglions (TGs). CFA treatment significantly increased the mRNA expression of NLRP3, interleukin (IL)-1β, and IL-18 in TGs compared to the control group. Moreover, 26 miRs were differentially expressed in TGs from trigeminal neuropathic pain mice, and the expression of miR-186...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orofacial pain; Trigeminal ganglion; NLRP3 inflammasome; MiR-186; Inflammation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900607

Chronic granulomatous disease: why an inflammatory disease?
Provedor de dados: 56
Autores: Roxo-Junior,P.; Simão,H.M.L.
Chronic granulomatous disease is a primary immunodeficiency caused by mutations in the genes encoding subunits of the phagocytic NADPH oxidase system. Patients can present with severe, recurrent infections and noninfectious conditions. Among the latter, inflammatory manifestations are predominant, especially granulomas and colitis. In this article, we systematically review the possible mechanisms of hyperinflammation in this rare primary immunodeficiency condition and their correlations with clinical aspects.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic granulomatous disease; Inflammation; Infection; Granulomas; NADPH oxidase.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100924

The C-peptide response to a standard mixed meal in a group of Brazilian type 1 diabetic patients
Provedor de dados: 56
Autores: Pozzan,R.; Dimetz,T.; Gazzola,H.M.; Gomes,M.B.
In order to analyze the different parameters used in the interpretation of C-peptide response in a functional test, we compared a group of 26 type 1 diabetics aged 21.1 ± 8.2 years, with a diabetes duration of 7.9 ± 6.7 months, with a group of 24 non-diabetic subjects aged 25.0 ± 4.4 years. A standard mixed meal of 317 kcal was used as a stimulus. Blood sampling for C-peptide determinations was performed at regular intervals. Although all the studied C-peptide variables were significantly lower in the diabetic group (P&amp;lt;0.0001), some overlapping of parameters was observed between the two groups. The highest degree of overlapping was found for basal value (BV) (30.8%) and percent increase (42.31%), and the lowest for incremental area, absolute...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 1 diabetes mellitus; C-peptide response; Mixed meal test; Beta cell function.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000005

Tissue expression and subcellular localization of Mipu1, a novel myocardial ischemia-related gene
Provedor de dados: 56
Autores: Wang,G.; Zuo,X.; Jiang,L.; Wang,K.; Wei,X.; Zhang,B.; Xiao,X.
Myocardial ischemic preconditioning up-regulated protein 1 (Mipu1), a novel zinc finger protein, was originally cloned using bioinformatic analysis and 5' RACE technology of rat heart after a transient myocardial ischemia/reperfusion procedure in our laboratory. In order to investigate the functions of Mipu1, the recombinant prokaryotic expression vector pQE31-Mipu1 was constructed and transformed into Escherichia coli M15(pREP4), and Mipu1-6His fusion protein was expressed and purified. The identity of the purified protein was confirmed by mass spectrometry. The molecular mass of the Mipu1 protein was 70.03779 kDa. The fusion protein was intracutaneously injected to immunize New Zealand rabbits to produce a polyclonal antibody. The antibody titer was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mipu1; Purification; Expression; Polyclonal antibody.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100007

Corticosteroids in acute respiratory distress syndrome
Provedor de dados: 56
Autores: Fernandes,A.B.S.; Zin,W.A.; Rocco,P.R.M.
Improving the course and outcome of patients with acute respiratory distress syndrome presents a challenge. By understanding the immune status of a patient, physicians can consider manipulating proinflammatory systems more rationally. In this context, corticosteroids could be a therapeutic tool in the armamentarium against acute respiratory distress syndrome. Corticosteroid therapy has been studied in three situations: prevention in high-risk patients, early treatment with high-dose, short-course therapy, and prolonged therapy in unresolving cases. There are differences between the corticosteroid trials of the past and recent trials: today, treatment starts 2-10 days after disease onset in patients that failed to improve; in the past, the corticosteroid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute respiratory distress syndrome; Collagen; Cytokine; Methylprednisolone; Experimental models; Clinical trials.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200003

Decellularization of placentas: establishing a protocol
Provedor de dados: 56
Autores: Leonel,L.C.P.C.; Miranda,C.M.F.C.; Coelho,T.M.; Ferreira,G.A.S.; Caãada,R.R.; Miglino,M.A.; Lobo,S.E.
Biological biomaterials for tissue engineering purposes can be produced through tissue and/or organ decellularization. The remaining extracellular matrix (ECM) must be acellular and preserve its proteins and physical features. Placentas are organs of great interest because they are discarded after birth and present large amounts of ECM. Protocols for decellularization are tissue-specific and have not been established for canine placentas yet. This study aimed at analyzing a favorable method for decellularization of maternal and fetal portions of canine placentas. Canine placentas were subjected to ten preliminary tests to analyze the efficacy of parameters such as the type of detergents, freezing temperatures and perfusion. Two protocols were chosen for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Placenta; Decellularization; Acellular biomaterial.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100601

A mechanistic view of mitochondrial death decision pores
Provedor de dados: 56
Autores: Belizário,J.E.; Alves,J.; Occhiucci,J.M.; Garay-Malpartida,M.; Sesso,A.
Mitochondria increase their outer and inner membrane permeability to solutes, protons and metabolites in response to a variety of extrinsic and intrinsic signaling events. The maintenance of cellular and intraorganelle ionic homeostasis, particularly for Ca2+, can determine cell survival or death. Mitochondrial death decision is centered on two processes: inner membrane permeabilization, such as that promoted by the mitochondrial permeability transition pore, formed across inner membranes when Ca2+ reaches a critical threshold, and mitochondrial outer membrane permeabilization, in which the pro-apoptotic proteins BID, BAX, and BAK play active roles. Membrane permeabilization leads to the release of apoptogenic proteins: cytochrome c, apoptosis-inducing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial outer membrane permeabilization; Permeability transition pore; Cytochrome c; Reactive oxygen species; Caspases; BCL-2.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800001

The effect of a low dose of clenbuterol on rat soleus muscle submitted to joint immobilization
Provedor de dados: 56
Autores: Cancelliero,K.M.; Durigan,J.L.Q.; Vieira,R.P.; Silva,C.A.; Polacow,M.L.O.
The aim of the present study was to evaluate the effect of joint immobilization on morphometric parameters and glycogen content of soleus muscle treated with clenbuterol. Male Wistar (3-4 months old) rats were divided into 4 groups (N = 6 for each group): control, clenbuterol, immobilized, and immobilized treated with clenbuterol. Immobilization was performed with acrylic resin orthoses and 10 µg/kg body weight clenbuterol was administered subcutaneously for 7 days. The following parameters were measured the next day on soleus muscle: weight, glycogen content, cross-sectional area, and connective tissue content. The clenbuterol group showed an increase in glycogen (81.6%, 0.38 ± 0.09 vs 0.69 ± 0.06 mg/100 g; P &lt; 0.05) without alteration in weight,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immobilization; Clenbuterol; Connective tissue; Glycogen content; Morphometry; Rat soleus muscle.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200003

Functional characterization and localization of AQP3 in the human colon
Provedor de dados: 56
Autores: Silberstein,C.; Kierbel,A.; Amodeo,G.; Zotta,E.; Bigi,F.; Berkowski,D.; Ibarra,C.
Water channels or aquaporins (AQPs) have been identified in a large variety of tissues. Nevertheless, their role in the human gastrointestinal tract, where their action is essential for the reabsorption and secretion of water and electrolytes, is still unclear. The purpose of the present study was to investigate the structure and function of water channels expressed in the human colon. A cDNA fragment of about 420 bp with a 98% identity to human AQP3 was amplified from human stomach, small intestine and colon by reverse transcription polymerase chain reaction (RT-PCR) and a transcript of 2.2 kb was expressed more abundantly in colon than in jejunum, ileum and stomach as indicated by Northern blots. Expression of mRNA from the colon of adults and children...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Absorptive epithelium; Human colon; Water channels; Aquaporins; AQP3.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000018

Assessment of the diagnostic value of diffusion tensor imaging in patients with spinal cord compression: a meta-analysis
Provedor de dados: 56
Autores: Li,X.F.; Yang,Y.; Lin,C.B.; Xie,F.R.; Liang,W.G.
We investigated the diagnostic value of the apparent diffusion coefficient (ADC) and fractional anisotropy (FA) of magnetic resonance diffusion tensor imaging (DTI) in patients with spinal cord compression (SCC) using a meta-analysis framework. Multiple scientific literature databases were exhaustively searched to identify articles relevant to this study. Mean values and standardized mean differences (SMDs) were calculated for the ADC and FA in normal and diseased tissues. The STATA version 12.0 software was used for statistical analysis. Of the 41 articles initially retrieved through database searches, 11 case-control studies were eligible for the meta-analysis and contained a combined total of 645 human subjects (394 patients with SCC and 251 healthy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diffusion tensor imaging; Apparent diffusion coefficient; Fractional anisotropy; Spinal cord compression; Meta-analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100704

Effects of strain and age on ear wound healing and regeneration in mice
Provedor de dados: 56
Autores: Costa,R.A.; Ruiz-de-Souza,V.; Azevedo Jr.,G.M.; Vaz,N.M.; Carvalho,C.R.
Round holes in the ears of MRL mice tend to close with characteristics of regeneration believed to be absent in other mouse strains (e.g., C57BL/6). We evaluated the kinetics and the histopathology of ear wound closure in young (8 weeks old) C57BL/6 and BALB/c mice. We also used middle-aged (40 weeks old) C57BL/6 mice to evaluate the influence of aging on this process. A circular through-and-through hole was made in the ear, photographs were taken at different times after injury and wound area was measured with digital analysis software. The percentages of closed area measured on day 100 were: 23.57 ± 8.66% for young BALB/c mice, 56.47 ± 7.39% for young C57BL/6 mice, and 75.31 ± 23.65% for middle-aged C57BL/6 mice. Mice were sacrificed on days 1, 3, 5, 25,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Wound healing; Regeneration; Scar; Aging; C57BL/6 mice; BALB/c mice.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200004

Cardiovascular responses to locomotor activity and feeding in unrestrained three-toed sloths, Bradypus variegatus
Provedor de dados: 56
Autores: Duarte,D.P.F.; Jaguaribe,A.M.; Pedrosa,M.A.C.; Clementino,A.C.C.R.; Barbosa,A.A.; Silva,A.F.V.; Gilmore,D.P.; Da Costa,C.P.
Heart rate (HR) and systolic (SBP), diastolic (DBP) and mean (MBP) blood pressure were recorded by biotelemetry in nine conscious unrestrained sloths for 1 min every 15 min over a 24-h period. The animals were allowed to freely move in an acoustically isolated and temperature-controlled (24 ± 1ºC) experimental room with light-dark cycle (12/12 h). Behavior was closely monitored through a unidirectional visor and classified as resting (sitting or suspended), feeding (chewing and swallowing embauba leaves, Cecropia adenops), or locomotor activity around the tree trunk or on the room floor. Locomotor activity caused statistically significant increases in SBP (+8%, from 121 ± 22 to 131 ± 18 mmHg), DBP (+7%, from 86 ± 17 to 92 ± 10 mmHg), MBP (+8%, from 97 ± 19...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tree-toed sloth; Heart rate; Blood pressure; Locomotor activity; Feeding behavior.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000016

GM1 improves neurofascin155 association with lipid rafts and prevents rat brain myelin injury after hypoxia-ischemia
Provedor de dados: 56
Autores: Zhang,Y.P.; Huang,Q.L.; Zhao,C.M.; Tang,J.L.; Wang,Y.L.
White matter injury characterized by damage to myelin is an important process in hypoxic-ischemic brain damage (HIBD). Because the oligodendrocyte-specific isoform of neurofascin, neurofascin 155 (NF155), and its association with lipid rafts are essential for the establishment and stabilization of the paranodal junction, which is required for tight interaction between myelin and axons, we analyzed the effect of monosialotetrahexosyl ganglioside (GM1) on NF155 expression and its association with lipid rafts after HIBD in Sprague-Dawley rats, weighing 12-15 g, on day 7 post-partum (P7; N = 20 per group). HIBD was induced on P7 and the rats were divided into two groups: one group received an intraperitoneal injection of 50 mg/kg GM1 three times and the other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebral hypoxia; Cerebral ischemia; Ganglioside; Myelin; Neurofascin 155.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600009

Primary and secondary esophageal contractions in patients with gastroesophageal reflux disease
Provedor de dados: 56
Autores: Aben-Athar,C.G.; Dantas,R.O.
We studied the primary and secondary esophageal peristalsis in 36 patients with heartburn and acid regurgitation and in 14 asymptomatic volunteers. Primary peristalsis was elicited by ten swallows of a 5-mL bolus of water and secondary peristalsis was elicited by intra-esophageal infusion of 5, 10, and 15 mL water, 0.1 N hydrochloric acid and air. Esophageal contractions were measured by an 8-lumen manometric catheter assembly incorporating a 6-cm sleeve device. Contractions were registered at 3, 9, and 15 cm from the upper margin of the sleeve and the infusion was done through a side hole located at 12 cm. Twenty patients had normal endoscopic esophageal examination, 10 with normal (group I) and 10 with abnormal pH-metric examination (group II), and 16...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esophageal contractions; Gastroesophageal reflux disease; Esophagitis; Heartburn; Esophageal motility.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800005

Effects of reversible inactivation of the dorsomedial hypothalamus on panic- and anxiety-related responses in rats
Provedor de dados: 56
Autores: Nascimento,J.O.G.; Zangrossi Jr.,H.; Viana,M.B.
The medial hypothalamus is part of a neurobiological substrate controlling defensive behavior. It has been shown that a hypothalamic nucleus, the dorsomedial hypothalamus (DMH), is involved in the regulation of escape, a defensive behavior related to panic attacks. The role played by the DMH in the organization of conditioned fear responses, however, is less clear. In the present study, we investigated the effects of reversible inactivation of the DMH with the GABA A agonist muscimol on inhibitory avoidance acquisition and escape expression by male Wistar rats (approximately 280 g in weight) tested in the elevated T-maze (ETM). In the ETM, inhibitory avoidance, a conditioned defensive response, has been associated with generalized anxiety disorder. Results...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Muscimol; Generalized anxiety disorder; Panic disorder; Dorsomedial hypothalamus; Elevated T-maze.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900009

Gender-dependent effects of aging on the kidney
Provedor de dados: 56
Autores: Gava,A.L.; Freitas,F.P.S.; Meyrelles,S.S.; Silva,I.V.; Graceli,J.B.
It is well known that the kidney plays an important role in the development of cardiovascular diseases such as hypertension. The normal aging process leads to changes in kidney morphology, hemodynamics and function, which increase the incidence of cardiovascular events in the elderly population. These disturbances are influenced by several factors, including gender. In general, females are protected by the effects of estrogens on the cardiorenal system. Several studies have demonstrated the beneficial effects of estrogens on renal function in the elderly; however, the relationships between androgens and kidney health during one’s lifetime are not well understood. Sex steroids have many complex actions, and the decline in their levels during aging clearly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sexual dimorphism; Renal function; Aging; Cardiovascular disorders; Sex hormones.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900012

Effect of acute nitric oxide synthase inhibition in the modulation of heart rate in rats
Provedor de dados: 56
Autores: Fellet,A.L.; Di Verniero,C.; Arza,P.; Tomat,A.; Varela,A.; Arranz,C.; Balaszczuk,A.M.
Acute nitric oxide synthase inhibition with N G-nitro-L-arginine methyl ester (L-NAME) on chronotropic and pressor responses was studied in anesthetized intact rats and rats submitted to partial and complete autonomic blockade. Blood pressure and heart rate were monitored intra-arterially. Intravenous L-NAME injection (7.5 mg/kg) elicited the same hypertensive response in intact rats and in rats with partial (ganglionic and parasympathetic blockade) and complete autonomic blockade (38 ± 3, 55 ± 6, 54 ± 5, 45 ± 5 mmHg, respectively; N = 9, P = NS). L-NAME-induced bradycardia at the time when blood pressure reached the peak plateau was similar in intact rats and in rats with partial autonomic blockade (43 ± 8, 38 ± 5, 46 ± 6 bpm, respectively; N = 9, P =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Nitric oxide synthase; Autonomic nervous system; Heart rate; Heart.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500015

Correlation between serum 25-hydroxyvitamin D levels and carotid intima-media thickness in a Brazilian population descended from African slaves
Provedor de dados: 56
Autores: Monteiro Júnior,F.C.; Mandarino,N.R.; Santos,E.M.; Santos,A.M.; Salgado,J.V.; Brito,D.J.A.; Salgado,B.J.L.; Lages,J.S.; Castelo Branco,G.; Salgado Filho,N.
Hypovitaminosis D has been identified as a possible new cardiovascular risk factor. However, the results of studies correlating serum vitamin D levels with markers of subclinical atherosclerosis have been conflicting. The aim of this study was to correlate serum levels of 25-hydroxyvitamin D [25(OH)D] with carotid intima-media thickness (C-IMT) and conventional cardiovascular risk factors in Afro-descendants. A cross-sectional analysis was performed on a sample of 382 individuals from a cohort of descendants of African slaves, inhabitants of “Quilombola” communities, with a mean age of 57.79 ±15.3 years, 54.5% of whom were women. Socio-demographic and clinical data were collected and biochemical tests were performed, including serum levels of 25(OH)D by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitamin D deficiency; Carotid intima-media thickness (C-IMT); Atherosclerosis; African continental ancestry group.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400606

Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas
Provedor de dados: 56
Autores: Veiga-Castelli,L.C.; Rosa e Silva,J.C.; Meola,J.; Ferriani,R.A.; Yoshimoto,M.; Santos,S.A.; Squire,J.A.; Martelli,L.
Endometriosis is a complex and multifactorial disease. Chromosomal imbalance screening in endometriotic tissue can be used to detect hot-spot regions in the search for a possible genetic marker for endometriosis. The objective of the present study was to detect chromosomal imbalances by comparative genomic hybridization (CGH) in ectopic tissue samples from ovarian endometriomas and eutopic tissue from the same patients. We evaluated 10 ovarian endometriotic tissues and 10 eutopic endometrial tissues by metaphase CGH. CGH was prepared with normal and test DNA enzymatically digested, ligated to adaptors and amplified by PCR. A second PCR was performed for DNA labeling. Equal amounts of both normal and test-labeled DNA were hybridized in human normal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endometrioma; Endometriosis; Comparative genomic hybridization; Chromosomal imbalances.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800014

Nitric oxide regulates angiotensin-I converting enzyme under static conditions but not under shear stress
Provedor de dados: 56
Autores: Pertrini,C.M.; Miyakawa,A.A.; Laurindo,F.R.M.; Krieger,J.E.
Mechanical forces including pressure and shear stress play an important role in vascular homeostasis via the control of the production and release of a variety of vasoactive factors. An increase in vascular shear stress is accompanied by nitric oxide (NO) release and NO synthase activation. Previously, we have demonstrated that shear stress induces angiotensin-I converting enzyme (ACE) down-regulation in vivo and in vitro. In the present study, we determined whether NO participates in the shear stress-induced ACE suppression response. Rabbit aortic endothelial cells were evaluated using the NO synthase inhibitor L-NAME, and two NO donors, diethylamine NONOate (DEA/NO) and sodium nitroprusside (SNP). Under static conditions, incubation of endothelial cells...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Shear stress; Angiotensin-I converting enzyme; Nitric oxide; Endothelial cells.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900005

Is the Thoroughbred race-horse under chronic stress?
Provedor de dados: 56
Autores: Nogueira,G.P.; Barnabe,R.C.
Thoroughbred fillies were divided into three groups according to age: group 1, 7 fillies aged 1 to 2 years (G1) starting the training program; group 2, 9 fillies aged 2 to 3 years (G2) in a full training program; group 3, 8 older fillies 3 to 4 years of age (G3) training and racing. Blood samples were collected weekly from July to December. Cortisol was quantified using a solid phase DPC kit. The intra- and interassay coefficients of variation were 12.5% and 15.65% and sensitivity was 1.9 ± 0.2 nmol/l. The semester average of cortisol levels varied between groups: G1 = 148.8 ± 6.7, G2 = 125.7 ± 5.8, G3 = 101.1 ± 5.4 nmol/l, with G3 differing statistically from the other groups. The lower cortisol levels observed in the older fillies lead us to propose that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horses; Cortisol; Exercise; Chronic stress.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000016

Tonic and reflex cardiovascular autonomic control in trained-female rats
Provedor de dados: 56
Autores: Sanches,I.C.; Sartori,M.; Jorge,L.; Irigoyen,M.C.; De Angelis,K.
The effects of exercise training on cardiovascular and autonomic functions were investigated in female rats. After an aerobic exercise training period (treadmill: 5 days/week for 8 weeks), conscious female Wistar (2 to 3 months) sedentary (S, N = 7) or trained rats (T, N = 7) were cannulated for direct arterial pressure (AP) recording in the non-ovulatory phases. Vagal (VT) and sympathetic tonus (ST) were evaluated by vagal (atropine) and sympathetic (propranolol) blockade. Baroreflex sensitivity was evaluated by the heart rate responses induced by AP changes. Cardiopulmonary reflex was measured by the bradycardic and hypotensive responses to serotonin. Resting bradycardia was observed in T (332 ± 7 bpm) compared with S animals (357 ± 10 bpm), whereas AP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baroreflex; Cardiopulmonary reflex; Exercise training; Female; Heart rate.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000011

Ischemia and fibrosis: the risk mechanisms of hypertensive heart disease
Provedor de dados: 56
Autores: Frohlich,E.D.
Mechanisms underlying risk associated with hypertensive heart disease (HHD) and left ventricular hypertrophy (LVH) are discussed in this report and provide a rationale for understanding this very common and important cause of death from hypertension and its complications. Emphasized are impaired coronary hemodynamics, endothelial dysfunction, and ventricular fibrosis from increased collagen deposition intramurally and perivascularly. Each is exacerbated by aging and, perhaps, also by increased dietary salt intake. These functional and structural changes promote further endothelial dysfunction, altered coronary hemodynamics, and diastolic as well as systolic ventricular contractile function in HHD. The clinical endpoints of HHD include angina pectoris (with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Left ventricular hypertrophy; LVH; Risk factor; Myocardial ischemia; Ventricular fibrosis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600010

Negative emotions affect postoperative scores for evaluating functional knee recovery and quality of life after total knee replacement
Provedor de dados: 56
Autores: Qi,A.; Lin,C.; Zhou,A.; Du,J.; Jia,X.; Sun,L.; Zhang,G.; Zhang,L.; Liu,M.
This study aimed to determine whether psychological factors affect health-related quality of life (HRQL) and recovery of knee function in total knee replacement (TKR) patients. A total of 119 TKR patients (male: 38; female: 81) completed the Beck Anxiety Inventory (BAI), Beck Depression Inventory (BDI), State Trait Anxiety Inventory (STAI), Eysenck Personality Questionnaire-revised (EPQR-S), Knee Society Score (KSS), and HRQL (SF-36). At 1 and 6 months after surgery, anxiety, depression, and KSS scores in TKR patients were significantly better compared with those preoperatively (P&lt;0.05). SF-36 scores at the sixth month after surgery were significantly improved compared with preoperative scores (P&lt;0.001). Preoperative Physical Component Summary Scale...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Total knee replacement; Negative emotion; Anxiety; Depression; Functional recovery; Health-related quality of life.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100602

Association between polymorphisms in the APOB gene and hyperlipidemia in the Chinese Yugur population
Provedor de dados: 56
Autores: Gu,Q-L.; Han,Y.; Lan,Y-M.; Li,Y.; Kou,W.; Zhou,Y-S.; Hai,X-J.; Yan,B.; Ci,C-H.
We investigated the influence of apolipoprotein B gene (APOB) variants on the risk of hyperlipidemia (HL) in 631 middle-aged and elderly members of the Chinese Yugur population (HL, n=336; normolipidemia, n=295). APOB polymorphisms were identified using mass spectrometry, and five single nucleotide polymorphisms (rs1042034, rs2163204, rs512535, rs676210, and rs679899) and serum lipids were further analyzed. rs1042034 and rs676210 were significantly associated with HL (P&lt;0.05). Compared with the GG or AA genotype, individuals with AG and AG+AA in rs1042034 and with AG and AG+GG in rs676210 had a 1.67-fold (95%CI=1.20–2.33),1.63-fold (95%CI=1.19–2.24), 1.72-fold (95%CI=1.24–2.40), and 1.67-fold (95%CI=1.21–2.291) increased risk of high HL, respectively....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yugur minority; Apolipoprotein B gene; Single nucleotide polymorphism; Hyperlipidemia; Serum lipids.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100601

The modulation of simple reaction time by the spatial probability of a visual stimulus
Provedor de dados: 56
Autores: Carreiro,L.R.R.; Haddad Jr.,H.; Baldo,M.V.C.
Simple reaction time (SRT) in response to visual stimuli can be influenced by many stimulus features. The speed and accuracy with which observers respond to a visual stimulus may be improved by prior knowledge about the stimulus location, which can be obtained by manipulating the spatial probability of the stimulus. However, when higher spatial probability is achieved by holding constant the stimulus location throughout successive trials, the resulting improvement in performance can also be due to local sensory facilitation caused by the recurrent spatial location of a visual target (position priming). The main objective of the present investigation was to quantitatively evaluate the modulation of SRT by the spatial probability structure of a visual...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Simple reaction time; Spatial probability; Vision; Attention.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700011

Neural regulation of the stress response: glucocorticoid feedback mechanisms
Provedor de dados: 56
Autores: Herman,J.P.; McKlveen,J.M.; Solomon,M.B.; Carvalho-Netto,E.; Myers,B.
The mammalian stress response is an integrated physiological and psychological reaction to real or perceived adversity. Glucocorticoids are an important component of this response, acting to redistribute energy resources to both optimize survival in the face of challenge and to restore homeostasis after the immediate challenge has subsided. Release of glucocorticoids is mediated by the hypothalamo-pituitary-adrenal (HPA) axis, driven by a neural signal originating in the paraventricular nucleus (PVN). Stress levels of glucocorticoids bind to glucocorticoid receptors in multiple body compartments, including the brain, and consequently have wide-reaching actions. For this reason, glucocorticoids serve a vital function in negative feedback inhibition of their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypothalamo-pituitary-adrenocortical axis; Corticotropin-releasing hormone; Glucocorticoid receptor; Amygdala; Hippocampus; Prefrontal cortex.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400002

Training graduate students to be teachers
Provedor de dados: 56
Autores: de-Macedo,D.V.; de-Paula,E.; Torres,B.B.
Pedagogic education of graduate students, when and where it exists, is restricted to theoretical courses or to the participation of the students as teachers' assistants. This model is essentially reproductive and offers few opportunities for any significant curriculum innovation. To open an opportunity for novelty we have introduced a new approach in "Biochemistry Teaching", a course included in the Biochemistry Graduate Program of the Biochemistry Department (Universidade Estadual de Campinas and Universidade de São Paulo). The content of the course consists of a) choosing the theme, b) selecting and organizing the topics, c) preparing written material, d) establishing the methodological strategies, e) planning the evaluation tools and, finally, f) as...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pedagogic training; Tertiary-level education.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200002

Transitory increased blood pressure after upper airway surgery for snoring and sleep apnea correlates with the apnea-hypopnea respiratory disturbance index
Provedor de dados: 56
Autores: Araújo,M.T.M.; Ouayoun,M.; Poirier,J.M.; Bayle,M.M.; Vasquez,E.C.; Fleury,B.
A transitory increase in blood pressure (BP) is observed following upper airway surgery for obstructive sleep apnea syndrome but the mechanisms implicated are not yet well understood. The objective of the present study was to evaluate changes in BP and heart rate (HR) and putative factors after uvulopalatopharyngoplasty and septoplasty in normotensive snorers. Patients (N = 10) were instrumented for 24-h ambulatory BP monitoring, nocturnal respiratory monitoring and urinary catecholamine level evaluation one day before surgery and on the day of surgery. The influence of postsurgery pain was prevented by analgesic therapy as confirmed using a visual analog scale of pain. Compared with preoperative values, there was a significant (P &lt; 0.05) increase in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snorers; Obstructive sleep apnea syndrome; Uvulopalatopharyngoplasty; Septoplasty; Blood pressure; Sympathetic activity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200017

Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin
Provedor de dados: 56
Autores: Bracesco,N.; Sosa,V.; Blanc,L.; Contreras,V.; Candreva,E.C.; Salvo,V.A.; Hocart,S.; Mechoso,B.; Nunes,E.
DNA repair pathways, cell cycle checkpoints, and redox protection systems are essential factors for securing genomic stability. The aim of the present study was to analyze the effect of Ilex paraguariensis (Ip) infusion and one of its polyphenolic components rutin on cellular and molecular damage induced by ionizing radiation. Ip is a beverage drank by most inhabitants of Argentina, Paraguay, Southern Brazil, and Uruguay. The yeast Saccharomyces cerevisiae (SC7Klys 2-3) was used as the eukaryotic model. Exponentially growing cells were exposed to gamma rays (γ) in the presence or absence of Ip or rutin. The concentrations used simulated those found in the habitual infusion. Surviving fractions, mutation frequency, and DNA double-strand breaks (DSB) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiation damage; Ilex paraguariensis infusion; Rutin; Radioprotection; Antimutagenic effects; Saccharomyces cerevisiae.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900606

Dietary fiber, energy intake and nutritional status during the treatment of children with chronic constipation
Provedor de dados: 56
Autores: Speridião,P.G.L.; Tahan,S.; Fagundes-Neto,U.; Morais,M.B.
The present prospective study was carried out to determine dietary fiber and energy intake and nutritional status of children during the treatment of chronic constipation. Twenty-five patients aged 2 to 12 years with chronic constipation were submitted to clinical evaluation, assessment of dietary patterns, and anthropometry before and after 45 and 90 days of treatment. The treatment of chronic constipation included rectal disimpaction, ingestion of mineral oil and diet therapy. The standardized diet prescribed consisted of regular food without a fiber supplement and met the nutrient requirements according to the recommended daily allowance. The fiber content was 9.0 to 11.9 g for patients aged less than 6 years and 12.0 to 18.0 g for patients older than 6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Constipation; Anthropometry; Nutritional status; Energy intake; Dietary fiber.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600011

Poly(trimethylene carbonate-co-L-lactide) electrospun scaffolds for use as vascular grafts
Provedor de dados: 56
Autores: Braghirolli,D.I.; Caberlon,B.; Gamba,D.; Petry,JFTC.; Dias,M.L.; Pranke,P.
Currently, there is great clinical need for suitable synthetic grafts that can be used in vascular diseases. Synthetic grafts have been successfully used in medium and large arteries, however, their use in small diameter vessels is limited and presents a high failure rate. In this context, the aim of this study was to develop tissue engineering scaffolds, using poly(trimethylene carbonate-co-L-lactide) (PTMCLLA), for application as small diameter vascular grafts. For this, copolymers with varying trimethylene carbonate/lactide ratios – 20/80, 30/70, and 40/60 – were submitted to electrospinning and the resulting scaffolds were evaluated in terms of their physicochemical and biological properties. The scaffolds produced with PTMCLLA 20/80, 30/70, and 40/60...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Poly(trimethylene carbonate-co-L-lactide); Vascular scaffolds; Mesenchymal stem cells; Endothelial progenitor cells; Smooth muscle cells.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800611

CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo
Provedor de dados: 56
Autores: Wang,X.M.; Gao,S.J.; Guo,X.F.; Sun,W.J.; Yan,Z.Q.; Wang,W.X.; Xu,Y.Q.; Lu,D.
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo. We used lentivirus-vector-based RNAi to knock down CIAPIN1 in nude mice bearing MDR breast cancer tumors and found that lentivirus-vector-mediated silencing of CIAPIN1 could efficiently and significantly inhibit tumor growth when combined with chemotherapy in vivo. Furthermore, Western blot analysis showed that both CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CIAPIN1 gene; Multidrug resistance; RNA interference; MDR1 gene; Breast neoplasms.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400273

Estrogen receptor 1 gene polymorphisms in premenopausal women: interaction between genotype and smoking on lipid levels
Provedor de dados: 56
Autores: Almeida,S.; Hutz,M.H.
Estrogen has multiple effects on lipid and lipoprotein metabolism. We investigated the association between the four common single nucleotide polymorphisms in the estrogen receptor 1 (ESR1) gene locus, -1989T&gt;G, +261G&gt;C, IVS1-397T&gt;C and IVS1-351A&gt;G, and lipid and lipoprotein levels in southern Brazilians. The sample consisted in 150 men and 187 premenopausal women. The women were considered premenopausal if they had regular menstrual bleeding within the previous 3 months and were 18-50 years of age. Exclusion criteria were pregnancy, secondary hyperlipidemia due to renal, hepatic or thyroid disease, and diabetes. Smoking status was self-reported; subjects were classified as never smoked and current smokers. DNA was amplified by PCR and was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Estrogen; Estrogen receptor 1 variants; Lipid levels; Smoking.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000007

Percutaneous sciatic nerve block with tramadol induces analgesia and motor blockade in two animal pain models
Provedor de dados: 56
Autores: Sousa,A.M.; Ashmawi,H.A.; Costa,L.S.; Posso,I.P.; Slullitel,A.
Local anesthetic efficacy of tramadol has been reported following intradermal application. Our aim was to investigate the effect of perineural tramadol as the sole analgesic in two pain models. Male Wistar rats (280-380 g; N = 5/group) were used in these experiments. A neurostimulation-guided sciatic nerve block was performed and 2% lidocaine or tramadol (1.25 and 5 mg) was perineurally injected in two different animal pain models. In the flinching behavior test, the number of flinches was evaluated and in the plantar incision model, mechanical and heat thresholds were measured. Motor effects of lidocaine and tramadol were quantified and a motor block score elaborated. Tramadol, 1.25 mg, completely blocked the first and reduced the second phase of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tramadol; Tramadol-N-oxide; Rats; Peripheral nerves; Local anesthesia; Analgesia.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200009

Polymorphism of the promoter region and exon 1 of the CTLA4 gene in endemic pemphigus foliaceus (fogo selvagem)
Provedor de dados: 56
Autores: Pavoni,D.P.; Cerqueira,L.B.; Roxo,V.M.M.S.; Petzl-Erler,M.L.
Endemic pemphigus foliaceus (EPF) is an autoimmune bullous skin disease characterized by acantholysis and antibodies against a desmosomal protein, desmoglein 1. Genetic and environmental factors contribute to development of this multifactorial disease. HLA class II and some cytokine gene polymorphisms are the only genetic markers thus far known to be associated with susceptibility to or protection from EPF. The cytotoxic T-lymphocyte antigen-4 gene (CTLA4) encodes a key immunoreceptor molecule that regulates and inhibits T-cell proliferation. It participates in the regulatory process controlling autoreactivity and therefore has been considered a strong candidate gene in autoimmune diseases. In the search for genes that might influence EPF pathogenesis, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CTLA4; Pemphigus; Fogo selvagem; Autoimmunity; Genetic susceptibility; Brazilian population.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900010

Role of mitochondrial ATP-sensitive potassium channel-mediated PKC-ε in delayed protection against myocardial ischemia/reperfusion injury in isolated hearts of sevoflurane-preconditioned rats
Provedor de dados: 56
Autores: Wang,C.; Hu,S.M.; Xie,H.; Qiao,S.G.; Liu,H.; Liu,C.F.
This study aimed to determine the role of mitochondrial adenosine triphosphate-sensitive potassium (mitoKATP) channels and protein kinase C (PKC)-ε in the delayed protective effects of sevoflurane preconditioning using Langendorff isolated heart perfusion models. Fifty-four isolated perfused rat hearts were randomly divided into 6 groups (n=9). The rats were exposed for 60 min to 2.5% sevoflurane (the second window of protection group, SWOP group) or 33% oxygen inhalation (I/R group) 24 h before coronary occlusion. The control group (CON) and the sevoflurane group (SEVO) group were exposed to 33% oxygen and 2.5% sevoflurane for 60 min, respectively, without coronary occlusion. The mitoKATP channel inhibitor 5-hydroxydecanoate (5-HD) was given 30 min before...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ischemia; Reperfusion; MitoKatp channel; Preconditioning; Protein kinase C.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600528

Plasma cortisol levels in captive wild felines after chemical restraint
Provedor de dados: 56
Autores: Nogueira,G.P.; Silva,J.C.R.
Eight Panthera onca (Po), 13 Felis concolor (Fc), 7 Felis yagouaroundi (Fy), 7 Felis tigrina (Ft) and 5 Felis pardalis (Fp) specimens from São Paulo State zoos were used. All animals were restrained with darts containing 10 mg/kg ketamine and 1 mg/kg xylazine. Venous blood samples were collected as soon as possible (within 15-20 min) and serum was frozen until the time for cortisol quantification. Cortisol was determined using a solid phase radioimmunoassay with an intra-assay coefficient of 8.51%. Data were analyzed statistically by the Kruskal-Wallis test, followed by Dunn's multiple comparisons test, and the one-sample t-test, with the level of significance set at P&amp;lt;0.05. Data are reported as means ± SEM. Cortisol levels differed among the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wild felines; Plasma cortisol; Captive felines.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100016

Impact of HPV infection on the development of head and neck cancer
Provedor de dados: 56
Autores: Betiol,J.; Villa,L.L.; Sichero,L.
Human papillomavirus (HPV)-related head and neck squamous cell carcinoma (HNSCC) is considered to be a distinct clinical entity with better prognosis than the classical tobacco- and alcohol-associated tumors. The increasing incidence of this neoplasia during the last decades highlights the need to better understand the role of HPV in the development of these cancers. Although the proportion of HNSCC attributed to HPV varies considerably according to anatomical site, overall approximately 25% of all HNSCC are HPV-DNA positive, and HPV-16 is by far the most prevalent type. In this review we discuss the existing evidence for a causal association between HPV infection and HNSCC at diverse anatomical head and neck subsites.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Oncogenic potential; Head and neck cancer; Viral prevalence.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300217

Changes in expression of BDNF and its receptors TrkB and p75NTR in the hippocampus of a dog model of chronic alcoholism and abstinence
Provedor de dados: 56
Autores: Xu,R.; Duan,S.R.; Zhao,J.W.; Wang,C.Y.
Chronic ethanol consumption can produce learning and memory deficits. Brain-derived neurotrophic factor (BDNF) and its receptors affect the pathogenesis of alcoholism. In this study, we examined the expression of BDNF, tropomyosin receptor kinase B (TrkB) and p75 neurotrophin receptor (p75NTR) in the hippocampus of a dog model of chronic alcoholism and abstinence. Twenty domestic dogs (9-10 months old, 15-20 kg; 10 males and 10 females) were obtained from Harbin Medical University. A stable alcoholism model was established through ad libitum feeding, and anti-alcohol drug treatment (Zhong Yao Jie Jiu Ling, the main ingredient was the stems of watermelon; developed in our laboratory), at low- and high-doses, was carried out. The Zhong Yao Jie Jiu Ling was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic alcoholism; Brain-derived neurotrophic factor; Tropomyosin receptor kinase B; P75 Neurotrophin receptor; Immunohistochemistry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800703

MicroRNA-122 regulates caspase-8 and promotes the apoptosis of mouse cardiomyocytes
Provedor de dados: 56
Autores: Zhang,Z.W.; Li,H.; Chen,S.S.; Li,Y.; Cui,Z.Y.; Ma,J.
Cardiomyocyte apoptosis plays key roles in the pathogenesis of heart diseases such as myocardial infarction. MicroRNAs are important regulators of gene expression, which are also involved in the regulation of cardiomyocyte apoptosis. However, cardiomyocyte apoptosis regulated by microRNA (miR)-122 is largely unexplored. The aim of this study focused on the role of miR-122 in cardiomyocyte apoptosis. Cardiomyocytes were isolated from neonatal mice and primarily cultured. MiR-122 mimic and inhibitor were transfected to cardiomyocytes and verified by qRT-PCR. Cell viability and apoptosis post-transfection were assessed by MTT assay and flow cytometry, respectively. Changes in expression of caspase-8 were quantified by qRT-PCR and western blot. Results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiomyocyte; Apoptosis; MiR-122; Caspase-8; Myocardial infarction.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200601

Antinociceptive properties of the essential oil of Ocimum gratissimum L. (Labiatae) in mice
Provedor de dados: 56
Autores: Rabelo,M.; Souza,E.P.; Soares,P.M.G.; Miranda,A.V.; Matos,F.J.A.; Criddle,D.N.
We have investigated the antinociceptive effects of the essential oil of Ocimum gratissimum L. (Labiatae) (EOOG) in two classical models of pain in male Swiss mice (25-35 g), the writhing test and the formalin test. At doses of 30, 100 and 300 mg/kg (po), EOOG produced a dose-dependent inhibition (from 58.3 ± 4.4 to 40.7 ± 6.3, 36.4 ± 3.6 and 24.6 ± 3.6, respectively; N = 8-10, P&lt;0.05) of acetic acid-induced writhing, causing up to a ~60% inhibition at the highest dose used, comparable to that obtained with indomethacin (10 mg/kg, po). At the same doses, EOOG predominantly inhibited the late (inflammatory) phase of the formalin-induced pain response (from 59.3 ± 8.3 to 40.4 ± 4.8, 23.2 ± 2.8 and 25.3 ± 5.5, respectively; N = 6, P&lt;0.05), with a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ocimum gratissimum L.; Labiatae; Essential oil; Antinociception.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400016

Targeting and translocation of endothelial nitric oxide synthase
Provedor de dados: 56
Autores: Michel,T.
This review explores advances in our understanding of the intracellular regulation of the endothelial isoform of nitric oxide synthase (eNOS) in the context of its dynamically regulated subcellular targeting. Nitric oxide (NO) is a labile molecule, and may play important biological roles both within the cell in which it is synthesized and in its interactions with nearby cells and molecules. The localization of eNOS within the cell importantly influences the biological role and chemical fate of the NO produced by the enzyme. eNOS, a Ca2+/calmodulin-dependent enzyme, is subject to a complex pattern of intracellular regulation, including co- and post-translational modifications and interactions with other proteins and ligands. In endothelial cells and cardiac...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caveolin; Signal transduction; Nitric oxide synthase; Calmodulin; Estrogen; Bradykinin.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100006

Worker honeybee hemolymph lipid composition and synodic lunar cycle periodicities
Provedor de dados: 56
The aim of the present investigation was to extend a previous study, showing a correlation of the variations of hemolymph carbohydrates with synodic lunar-like cycle and its circaseptan harmonics to worker honeybee hemolymph lipids. Hemolymph lipid concentrations of emerging worker imagos were analyzed in terms of one ideal synodic lunar cycle and processed by the cosinor method testing the null hypothesis versus the presence of 29.5-, 14.8- or 7.4-day periods in the data. A rhythmicity statistically compatible with a 29.5-day rhythm was observed for triacylglycerols and steroids as well as for body weight. A circadiseptan rhythm was determined for 1,3 diacylglycerols, while fatty acids and phospholipids exhibited a circaseptan rhythm. An agreement of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Apis mellifera; Body weight; Fatty acids; Diacylglycerols; Triacylglycerols; Phospholipids; Steroids; Hemolymph; Synodic lunar cycle; Circa(di)septan subcycle.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200018

Apoptotic mimicry: an altruistic behavior in host/Leishmania interplay
Provedor de dados: 56
Autores: Wanderley,J.L.M.; Benjamin,A.; Real,F.; Bonomo,A.; Moreira,M.E.C.; Barcinski,M.A.
Apoptosis is the most common phenotype observed when cells die through programmed cell death. The morphologic and biochemical changes that characterize apoptotic cells depend on the activation of a diverse set of genes. Apoptosis is essential for multicellular organisms since their development and homeostasis are dependent on extensive cell renewal. In fact, there is strong evidence for the correlation between the emergence of multicellular organisms and apoptosis during evolution. On the other hand, no obvious advantages can be envisaged for unicellular organisms to carry the complex machinery required for programmed cell death. However, accumulating evidence shows that free-living and parasitic protozoa as well as yeasts display apoptotic markers. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Phosphatidylserine; Leishmania; Phagocyte.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600001

Induction of apoptosis in cancer cells by tumor necrosis factor and butyrolactone, an inhibitor of cyclin-dependent kinases
Provedor de dados: 56
Autores: Belizário,J.E.; Sherwood,S.; Beçak,W.
Induction of apoptosis by tumor necrosis factor (TNF) is modulated by changes in the expression and activity of several cell cycle regulatory proteins. We examined the effects of TNF (1-100 ng/ml) and butyrolactone I (100 µM), a specific inhibitor of cyclin-dependent kinases (CDK) with high selectivity for CDK-1 and CDK-2, on three different cancer cell lines: WEHI, L929 and HeLa S3. Both compounds blocked cell growth, but only TNF induced the common events of apoptosis, i.e., chromatin condensation and ladder pattern of DNA fragmentation in these cell lines. The TNF-induced apoptosis events were increased in the presence of butyrolactone. In vitro phosphorylation assays for exogenous histone H1 and endogenous retinoblastoma protein (pRb) in the total cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Cell cycle; Cyclin-dependent kinases; Cyclin-dependent kinase chemical inhibitors; Tumor necrosis factor.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400016

Sex-specific compensatory growth in food-deprived Nile tilapia
Provedor de dados: 56
Autores: Barreto,R.E.; Moreira,P.S.A.; Carvalho,R.F.
Female Nile tilapia incubate fertilized eggs in their mouth until they are released as alevins. Consequently, the female may not eat during this period. Thus, it would be expected that female Nile tilapia are more adapted to recovering from fasting than males, which do not display this behavior. To test this hypothesis we conducted an experiment with two groups of fish consisting of 7 males and 7 females each, with one fish per aquarium. The experiment was divided into three phases involving adjustment of the animals to experimental aquaria (0-15th day), fasting (16th-27th day), and refeeding (27th-42nd day). Compensatory growth performance was assessed by specific growth rate, weight, food conversion efficiency and food intake. Food conversion efficiency...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Compensatory growth; Fasting; Sex-related growth; Mouthbrooding fish; Nile tilapia; Oreochromis niloticus.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400009

Clinical and pathological challenges in the diagnosis of late-onset biliary atresia: four case studies
Provedor de dados: 56
Autores: Fontenele,J.P.U.; Schenka,A.A.; Hessel,G.; Jarry,V.M.; Escanhoela,C.A.F.
Biliary atresia (BA) is classically described at the neonatal age. However, rare cases of BA in older infants have also been reported. We report four cases of late-onset BA in infants older than 4 weeks (3 males, 1 female), and describe the diagnostic and management difficulties. One of the cases had a late-onset (29 weeks) presentation with a successful surgical procedure. We highlight the importance of this unusual differential diagnosis in infants with cholestatic syndrome, who may benefit from Kasai surgery, regardless of age.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Kasai operation; Cholestatic syndrome; Infants.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300501

Antifungal activity of the naphthoquinone beta-lapachone against disseminated infection with Cryptococcus neoformans var. neoformans in dexamethasone-immunosuppressed Swiss mice
Provedor de dados: 56
Autores: Medeiros,C.S.; Pontes-Filho,N.T.; Camara,C.A.; Lima-Filho,J.V.; Oliveira,P.C.; Lemos,S.A.; Leal,A.F.G.; Brandão,J.O.C.; Neves,R.P.
The in vivo antifungal activity of the naphthoquinone beta-lapachone against disseminated infection by Cryptococcus neoformans was investigated. Swiss mice were immunosuppressed daily with dexamethasone (0.5 mg per mouse) intraperitoneally for 3 days, the procedure was repeated 4 days later, and the animals were then challenged intravenously with C. neoformans (10(6) CFU/mL) 1 week later. Seven days after infection, the mice were divided into groups and treated daily with beta-lapachone (10 mg/kg, iv) for 7 (N = 6) and 14 days (N = 10). Amphotericin B (0.5 mg/kg) was used as comparator drug and an additional group received PBS. Treatment with beta-lapachone cleared the yeast from the spleen and liver, and the fungal burden decreased approximately 10(4)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Beta-lapachone; Cryptococcus neoformans; Cryptococcosis; Immunesuppression; Experimental infection; Amphotericin B.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400004

Successful scale-up of human embryonic stem cell production in a stirred microcarrier culture system
Provedor de dados: 56
Autores: Fernandes,A.M.; Marinho,P.A.N.; Sartore,R.C.; Paulsen,B.S.; Mariante,R.M.; Castilho,L.R.; Rehen,S.K.
Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES cells are mainly cultured in static tissue plates, which offer a limited surface and require repeated sub-culturing. Here we describe a stirred system with commercial dextran-based microcarriers coated with denatured collagen to scale-up hES cell production. Maintenance of pluripotency in the microcarrier-based stirred system was shown by immunocytochemical and flow cytometry analyses for pluripotency-associated markers. The formation of cavitated embryoid bodies expressing markers of endoderm, ectoderm and mesoderm was further evidence of maintenance of differentiation capability. Cell yield per volume of medium spent was more than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human embryonic stem cells; Scale-up; Microcarriers; Spinner bioreactors.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600007

Accuracy and prognostic ability of the SARC-F questionnaire and Ishii's score in the screening of sarcopenia in geriatric inpatients
Provedor de dados: 56
Autores: Li,Min; Kong,Yan; Chen,Hongcun; Chu,Aiqin; Song,Guiqi; Cui,Yan.
Sarcopenia remains poorly managed in clinical practice due to the lack of simple and accurate screening tools. This study aimed to identify the cutoff values of the SARC-F questionnaire and Ishii's score using the variables age, grip strength, and calf circumference in older inpatients in China to compare the accuracy of the two methods and to explore their predictive ability for adverse outcomes (rehospitalization, falls, fracture, and death). Hospitalized patients (n=138) aged ≥60 years were included. The accuracy of the two tools was evaluated using the reference diagnosis recommended by the Asian Working Group on Sarcopenia (assessing patients with measurements of muscle mass, handgrip strength, and usual gait speed). Follow-up data were obtained by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sarcopenia; Muscle mass; Geriatric inpatients; Screening; Score.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900606

The influence of fasting/refeeding on the lipoprotein lipase activity of adipose tissue and muscle
Provedor de dados: 56
Autores: Botion,L.M.
Lipoprotein lipase activity in adipose tissue and muscle is modulated by changes in the pattern of food intake. We have measured total lipoprotein lipase activity in adipose tissue and muscle of male Wistar rats (N = 6-10), weighing 200-250 g (~12 weeks), during the refeeding/fasting state following 24 h of fasting. Lipoprotein lipase activity in tissue homogenates was evaluated using a [³H]-triolein-containing substrate, and released [³H]-free fatty acids were extracted and quantified by liquid scintillation. Adipose tissue lipoprotein lipase activity did not completely recover within 2 h of refeeding (60% of refed ad libitum values). Cardiac lipoprotein lipase activity remained increased even 2 h after refeeding (100% of refed ad libitum values), whereas...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cardiac lipoprotein lipase; Adipose tissue lipoprotein lipase; Skeletal muscle lipoprotein lipase; Fasting.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100006

Bovine serum albumin potentiates caffeine- or ATP-induced tension in human skinned skeletal muscle fibers
Provedor de dados: 56
Human skinned muscle fibers were used to investigate the effects of bovine serum albumin (BSA) on the tension/pCa relationship and on the functional properties of the Ca2+-release channel of the sarcoplasmic reticulum (SR). In both fast- and slow-type fibers, identified by their tension response to pSr 5.0, BSA (0.7-15 µM) had no effect on the Ca2+ affinity of the contractile proteins and elicited no tension per se in Ca2+-loaded fibers. In contrast, BSA (&amp;gt;1.0 µM) potentiated the caffeine-induced tension in Ca2+-loaded fibers, this effect being more intense in slow-type fibers. Thus, BSA reduced the threshold caffeine concentration required for eliciting detectable tension, and increased the amplitude, the rate of rise and the area under the curve...
Palavras-chave: Calcium-release channel; Sarcoplasmic reticulum; Human muscle fiber; Bovine serum albumin; Caffeine.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500017

Vincristine delays gastric emptying and gastrointestinal transit of liquid in awake rats
Provedor de dados: 56
Autores: Peixoto Júnior,A.A.; Teles,B.C.V; Castro,E.F.B.; Santos,A.A.; de Oliveira,G.R.; Ribeiro,R.A.; Rola,F.H.; Gondim,F.A.A.
We evaluated the effects of vincristine on the gastrointestinal (GI) motility of awake rats and correlated them with the course of vincristine-induced peripheral neuropathy. Vincristine or saline was injected into the tail vein of male Wistar rats (180-250 g) on alternate days: 50 µg/kg (5 doses, N = 10), 100 µg/kg (2, 3, 4 and 5 doses, N = 49) or 150 µg/kg (1, 2, or 5 doses, N = 37). Weight and stool output were measured daily for each animal. One day after completing the vincristine treatment, the animals were fasted for 24 h, gavage-fed with a test meal and sacrificed 10 min later to measure gastric emptying (GE), GI transit and colon weight. Sensory peripheral neuropathy was evaluated by hot plate testing. Chronic vincristine treatments with total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Constipation; Gastrointestinal transit; Peripheral neuropathy; Rat gastric emptying; Vincristine.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600015

Effects of artepillin C on model membranes displaying liquid immiscibility
Provedor de dados: 56
Autores: Pazin,W.M.; Vilanova,N.; Voets,I.K.; Soares,A.E.E.; Ito,A.S.
It has been hypothesized that the therapeutic effects of artepillin C, a natural compound derived from Brazilian green propolis, are likely related to its partition in the lipid bilayer component of biological membranes. To test this hypothesis, we investigated the effects of the major compound of green propolis, artepillin C, on model membranes (small and giant unilamelar vesicles) composed of ternary lipid mixtures containing cholesterol, which display liquid-ordered (lo) and liquid-disordered (ld) phase coexistence. Specifically, we explored potential changes in relevant membrane parameters upon addition of artepillin C presenting both neutral and deprotonated states by means of small angle X-ray scattering (SAXS), differential scanning calorimetry...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artepillin C; Green propolis; Model membranes; Giant unilamellar vesicles; SAXS; DSC.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300610

RNAi-mediated knockdown of FANCF suppresses cell proliferation, migration, invasion, and drug resistance potential of breast cancer cells
Provedor de dados: 56
Autores: Zhao,L.; Li,N.; Yu,J.K.; Tang,H.T.; Li,Y.L.; He,M.; Yu,Z.J.; Bai,X.F.; Zheng,Z.H.; Wang,E.H.; Wei,M.J.
Fanconi anemia complementation group F protein (FANCF) is a key factor, which maintains the function of FA/BRCA, a DNA damage response pathway. However, the functional role of FANCF in breast cancer has not been elucidated. We performed a specific FANCF-shRNA knockdown of endogenous FANCF in vitro. Cell viability was measured with a CCK-8 assay. DNA damage was assessed with an alkaline comet assay. Apoptosis, cell cycle, and drug accumulation were measured by flow cytometry. The expression levels of protein were determined by Western blot using specific antibodies. Based on these results, we used cell migration and invasion assays to demonstrate a crucial role for FANCF in those processes. FANCF shRNA effectively inhibited expression of FANCF. We found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fanconi anemia complementation group F protein; Breast neoplasms; Tumor cell line.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100024

Acetylcholinesterase activity in the pons and medulla oblongata of rats after chronic electroconvulsive shock
Provedor de dados: 56
Autores: Camarini,R.; Benedito,M.A.C.
An imbalance between cholinergic and noradrenergic neurotransmission has been proposed for the etiology of affective disorders. According to this hypothesis, depression would be the result of enhanced cholinergic and reduced noradrenergic neurotransmission. Repeated electroconvulsive shock (ECS) is an effective treatment for depression; moreover, in laboratory animals it induces changes in brain noradrenergic neurotransmission similar to those obtained by chronic treatment with antidepressant drugs (down-regulation of beta-adrenergic receptors). The aim of the present study was to determine whether repeated ECS in rats changes acetylcholinesterase (Achase) activity. Achase controls the level of acetylcholine (Ach) in the synaptic cleft and its levels seem...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylcholinesterase; Medulla oblongata; Pons; Chronic electroconvulsive shock.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000012

Interleukin-27 augments the inhibitory effects of sorafenib on bladder cancer cells
Provedor de dados: 56
Autores: Cao,J.Y.; Yin,H.S.; Li,H.S.; Yu,X.Q.; Han,X.
Both sorafenib and interleukin-27 (IL-27) are antineoplastic drugs. This study aimed to investigate the synergistic effect of these two drugs on bladder cancer cells. HTB-9 and T24 cells were stimulated with IL-27 (50 ng/mL), sorafenib (2 μM) or the synergistic action of these two drugs. The cells without treatment acted as control. Cell proliferation, apoptosis and invasion were measured by bromodeoxyuridine assay, flow cytometry and modified Boyden chamber, respectively. Simultaneously, both modified Boyden chamber and scratch assay were used to assess cell migration. Finally, the phosphorylation levels of key kinases in the Akt/mechanistic target of rapamycin (mTOR)/mitogen-activated protein kinase (MAPK) pathway, and expression levels of matrix...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sorafenib; Interleukin 27; Proliferation; Apoptosis; Invasion; Akt/mTOR/MAPK.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800610

Induction of interleukin-10 by HIV antigens in peripheral mononuclear cells of health care workers after occupational exposure to HIV-1-positive blood
Provedor de dados: 56
Autores: Rodrigues,D.S.S.; Brunialti,M.K.C.; Medeiros,E.A.S.; Diaz,R.S.; Turcato,G.; Salomao,R.
Evaluation of HIV-induced IL-2 production by peripheral blood mononuclear cells (PBMC) and HIV-specific T helper and cytotoxic T lymphocyte (CTL) responses in health care workers (HCW) occupationally exposed to HIV reveals a high rate of response to HIV among non-seroconverters. IL-10 is also known to interfere with HIV infection in vitro. To evaluate the induction of IL-10 by HIV antigens in HCW occupationally exposed to HIV, 18 HCW with percutaneous injury were enrolled in this study, 9 of them exposed to HIV-contaminated blood, and 9 exposed to HIV-negative blood. PBMC were incubated on plates coated with HIV-1 antigens, and IL-10 was measured in supernatants by ELISA. Five of nine HCW exposed to HIV-contaminated blood presented HIV-induced IL-10. Two...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HIV-1; Interleukin-10; Needlestick injury; Occupational exposure; Health care worker.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600010

Implantation of muscle satellite cells overexpressing myogenin improves denervated muscle atrophy in rats
Provedor de dados: 56
Autores: Shen,H.; Lv,Y.; Shen,X.Q.; Xu,J.H.; Lu,H.; Fu,L.C.; Duan,T.
This study evaluated the effect of muscle satellite cells (MSCs) overexpressing myogenin (MyoG) on denervated muscle atrophy. Rat MSCs were isolated and transfected with the MyoG-EGFP plasmid vector GV143. MyoG-transfected MSCs (MTMs) were transplanted into rat gastrocnemius muscles at 1 week after surgical denervation. Controls included injections of untransfected MSCs or the vehicle only. Muscles were harvested and analyzed at 2, 4, and 24 weeks post-transplantation. Immunofluorescence confirmed MyoG overexpression in MTMs. The muscle wet weight ratio was significantly reduced at 2 weeks after MTM injection (67.17±6.79) compared with muscles injected with MSCs (58.83±5.31) or the vehicle (53.00±7.67; t=2.37, P=0.04 and t=3.39, P=0.007, respectively). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Denervation; Muscle atrophy; Satellite cells; Myogenin; Transplantation; Transfection.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200609

Motor behavioral abnormalities and histopathological findings of Wistar rats inoculated with HTLV-1-infected MT2 cells
Provedor de dados: 56
Autores: Câmara,C.C.; Oriá,R.B.; Felismino,T.C.; Silva,A.P. da; Silva,M.A.M. da; Alcântara,J.V.A.; Costa,S.B.C.; Vicente,A.C.P.; Teixeira-Santos,T.J.; Castro-Costa,C.M. de.
The objective of the present study was to describe motor behavioral changes in association with histopathological and hematological findings in Wistar rats inoculated intravenously with human T-cell lymphotropic virus type 1 (HTLV-1)-infected MT2 cells. Twenty-five 4-month-old male rats were inoculated with HTLV-1-infected MT2 cells and 13 control rats were inoculated with normal human lymphocytes. The behavior of the rats was observed before and 5, 10, 15, and 20 months after inoculation during a 30-min/rat testing time for 5 consecutive days. During each of 4 periods, a subset of rats was randomly chosen to be sacrificed in order to harvest the spinal cord for histopathological analysis and to obtain blood for serological and molecular studies....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Motor behaviors; HTLV-1-infected MT2 cells; Wistar rats; Experimental myelopathy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700008

An animal experimental study of porous magnesium scaffold degradation and osteogenesis
Provedor de dados: 56
Autores: Liu,Y.J.; Yang,Z.Y.; Tan,L.L.; Li,H.; Zhang,Y.Z.
Our objective was to observe the biodegradable and osteogenic properties of magnesium scaffolding under in vivo conditions. Twelve 6-month-old male New Zealand white rabbits were randomly divided into two groups. The chosen operation site was the femoral condyle on the right side. The experimental group was implanted with porous magnesium scaffolds, while the control group was implanted with hydroxyapatite scaffolds. X-ray and blood tests, which included serum magnesium, alanine aminotransferase (ALT), creatinine (CREA), and blood urea nitrogen (BUN) were performed serially at 1, 2, and 3 weeks, and 1, 2, and 3 months. All rabbits were killed 3 months postoperatively, and the heart, kidney, spleen, and liver were analyzed with hematoxylin and eosin (HE)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradable; Osteogenic; Magnesium scaffold.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800715

Daily blood pressure profile in Cushing's syndrome before and after surgery
Provedor de dados: 56
Autores: Kreze,A.; Mikulecky,M.
No significant difference has been demonstrated in the altered circadian blood pressure pattern between the pituitary-dependent and adrenal forms of Cushing's syndrome before surgery. The effect of therapy, however, proved to be different. The mesor was normalized in the pituitary-dependent Cushing's syndrome more conspicuously for systolic than for diastolic blood pressure. In Cushing's syndrome due to adrenal adenoma, systolic and diastolic blood pressure mesors have been even significantly "overnormalized" after treatment, being 11 to 27 and 2 to 13 mmHg (95% confidence) lower than corresponding mesors in controls. There was no difference between forms in the effect of treatment on blood pressure amplitudes, which remained significantly lower than in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cushing's syndrome; Pituitary-dependent Cushing's syndrome; Cushing's syndrome due to adrenal adenoma; Blood pressure; Circadian rhythm; Surgery.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000004

Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms
Provedor de dados: 56
Autores: Sakane,K.K.; Monteiro,C.J.; Silva,W.; Silva,A.R.; Santos,P.M.; Lima,K.F.; Moraes,K.C.M.
Cardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 µM celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellular and molecular analyses; Cell death; Cyclooxygenase-2 inhibitor; H9c2 cardiac cell line.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100050

Infrequent p53 gene alterations in ulcerative colitis
Provedor de dados: 56
Autores: Mattar,R.; Alexandrino,A.M.; Laudanna,A.A.
The purpose of this study was to determine whether point mutations and loss of the p53 gene take place in ulcerative colitis which is histologically negative for dysplasia. DNA was extracted from 13 frozen rectal or colon biopsies and blood samples. Ulcerative colitis was classified histologically as active (10 cases) and inactive (3 cases). Exons 5-8 were amplified by PCR, treated with exonuclease and shrimp alkaline phosphatase and sequenced by the dideoxy chain termination method with the Sequenase Version 2.0 DNA sequencing kit. PCR products of intron 6 and exon 4 were digested with MspI and AccII, respectively, for RFLP analysis. No p53 gene mutation was detected in these cases. The number of informative patients for loss of heterozygosity (LOH) at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P53 gene; Ulcerative colitis; LOH; Mutation.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900005

The clinical impact of MTHFR polymorphism on the vascular complications of sickle cell disease
Provedor de dados: 56
Autores: Moreira Neto,F.; Lourenço,D.M.; Noguti,M.A.E.; Morelli,V.M.; Gil,I.C.P.; Beltrão,A.C.S.; Figueiredo,M.S.
Sickle cell disease (SCD) is one of the most common inherited diseases in the world and the patients present notorious clinical heterogeneity. It is known that patients with SCD present activation of the blood coagulation and fibrinolytic systems, especially during vaso-occlusive crises, but also during the steady state of the disease. We determined if the presence of the factor V gene G1691A mutation (factor V Leiden), the prothrombin gene G20210A variant, and methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism may be risk factors for vascular complications in individuals with SCD. We studied 53 patients with SCD (60% being women), 29 with SS (sickle cell anemia; 28 years, range: 13-52 years) and 24 with SC (sickle-hemoglobin C disease; 38.5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sickle cell disease; SC hemoglobinopathy; Sickle hemoglobinopathies; Inherited hypercoagulation states; MTHFR polymorphism.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001000004

Use of conventional PCR and smear microscopy to diagnose pulmonary tuberculosis in the Amazonian rainforest area
Provedor de dados: 56
Autores: Carniel,F.; Dalla Costa,E.R.; Lima-Bello,G.; Martins,C.; Scherer,L.C.; Rossetti,M.L.
The diagnostic usefulness of Ziehl-Neelsen (ZN)-stained sputum smears combined with conventional polymerase chain reaction (ZN/PCR) to amplify IS6110 region DNA extracted from ZN slides was evaluated. The objective was to verify if this association could improve tuberculosis (TB) diagnosis in patients at remote sites. The study was carried out in 89 patients with culture-confirmed pulmonary TB as defined by the Brazilian Manual for TB Treatment. The participants were recruited in a reference unit for TB treatment in Rondônia, a state in the Amazonian area in northern Brazil. ZN, PCR, and culture performed in the sputum samples from these patients were analyzed in different combinations (i.e., ZN plus PCR and ZN plus culture). The prevalence rates of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Diagnosis; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201016

Absence of peripheral blood mononuclear cells priming in hemodialysis patients
Provedor de dados: 56
Autores: Santos,B.C.; Starobinas,N.; Barbuto,J.A.M.; Russo,M.; Schor,N.
As a consequence of the proinflammatory environment occurring in dialytic patients, cytokine overproduction has been implicated in hemodialysis co-morbidity. However, there are discrepancies among the various studies that have analyzed TNF-alpha synthesis and the presence of peripheral blood mononuclear cell (PBMC) priming in this clinical setting. We measured bioactive cytokine by the L929 cell bioassay, and evaluated PBMC TNF-alpha production by 32 hemodialysis patients (HP) and 51 controls. No difference in TNF-alpha secretion was observed between controls and HP (859 ± 141 vs 697 ± 130 U/10(6) cells). Lipopolysaccharide (5 µg/ml) did not induce any further TNF-alpha release, showing no PBMC priming. Paraformaldehyde-fixed HP PBMC were not cytotoxic to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokine; Stress response; Translational control; Transcriptional blockage; Priming; Membrane-anchored tumor necrosis factor.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200009

Effect of polymorphisms of the MTHFR and APOE genes on susceptibility to diabetes and severity of diabetic retinopathy in Brazilian patients
Provedor de dados: 56
Autores: Errera,F.I.V.; Silva,M.E.R.; Yeh,E.; Maranduba,C.M.C.; Folco,B.; Takahashi,W.; Pereira,A.C.; Krieger,J.E.; Passos-Bueno,M.R.
Diabetes mellitus (DM) is a highly prevalent complex genetic disorder. There has been a worldwide effort in the identification of susceptibility genes for DM and its complications, and the 5-10-methylenetetrahydrofolate reductase (MTHFR) and apolipoprotein-E (APOE) genes have been considered good candidate susceptibility genes to this condition. The objectives of the present study were to determine if the 677T MTHFR and epsilon2/epsilon3/epsilon4 APOE alleles are risk factors for DM and for severity of diabetic retinopathy (DR). A total of 248 individuals were studied: 107 healthy individuals and 141 diabetic patients (46 with type 1 diabetes and 95 with type 2 diabetes), who also had DR (81 with non-proliferative DR and 60 with proliferative DR). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: APOE; MTHFR; Polymorphisms; Diabetes; Diabetic retinopathy.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700005

Central lead administration inhibits water intake and sodium appetite in rats
Provedor de dados: 56
Autores: De-Castro-e-Silva,E.J.; Castro,L.; Luz,C.P.; Ferreira,H.; Lima,A.K.S.; Souza,F.S.F.; Maldonado,I.; Macêdo,D.F.; Ferreira,M.G.; Bandeira,I.P.V.; Amor,A.L.M.; Carvalho,F.L.Q.; Rocha Jr.,M.A.; Fregoneze,J.B.
We have demonstrated that acute third ventricle injections of lead acetate (PbAc) exert a powerful antidipsogenic effect and induce a significant increase in renal sodium excretion. In the present study we confirm the antidipsogenic effect of lead and demonstrate that central administration of this metal, in minute amounts, significantly reduces salt intake both during dehydration and after central angiotensinergic stimulation. Adult male Wistar rats had the third ventricle cannulated seven days before the experiments. During this period they had free access to distilled water and hypertonic saline solution (1.5%). After a 24-h period of fluid deprivation, experimental animals received third ventricle injections of PbAc (0.3, N = 8 and 3.0 nmol/rat, N =...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lead; Angiotensin II; Water intake; Sodium intake; Rats.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000011

Downregulation of survivin by siRNA inhibits invasion and promotes apoptosis in neuroblastoma SH-SY5Y cells
Provedor de dados: 56
Autores: Zhang,L.; Liang,H.; Cao,W.; Xu,R.; Ju,X.L.
Neuroblastoma is a solid tumor that occurs mainly in children. Malignant neuroblastomas have a poor prognosis because conventional chemotherapeutic agents are not very effective. Survivin, a member of the inhibitor of the apoptosis protein family, plays a significant role in cell division, inhibition of apoptosis, and promotion of cell proliferation and invasion. Previous studies found that survivin is highly expressed in some malignant neuroblastomas and is correlated with poor prognosis. The aim of this study was to investigate whether survivin could serve as a potential therapeutic target of human neuroblastoma. We employed RNA interference to reduce survivin expression in the human neuroblastoma SH-SY5Y cell line and analyzed the effect of RNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuroblastoma; Survivin; Apoptosis; Invasion; RNA interference.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700548

Lack of mutations of exon 2 of the MEN1 gene in endocrine and nonendocrine sporadic tumors
Provedor de dados: 56
Autores: Costa,S.C.; Nascimento,L.S.; Ferreira,F.J.; Mattos,P.S.; Camara-Lopes,L.H.; Ward,L.S.
In addition to the mutations that underlie most cases of the multiple endocrine neoplasia type 1 (MEN1) syndrome, somatic mutations of the MEN1 gene have also been described in sporadic tumors like gastrinomas, insulinomas and bronchial carcinoid neoplasm. We examined exon 2 of this gene, where most of the mutations have been described, in 148 endocrine and nonendocrine sporadic tumors. DNA was obtained by phenol/chloroform extraction and ethanol precipitation from 92 formalin-fixed, paraffin-embedded samples, and from 40 fresh tumor tissue samples. We used 5 pairs of primers to encompass the complete coding sequence of exon 2 of the MEN1 gene that was screened by the polymerase chain reaction-single-stranded conformation polymorphism (PCR-SSCP) technique...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: MEN1 gene; Sporadic tumors; Thyroid; Adrenal; Breast.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700004

Chronic converting enzyme inhibition normalizes QT interval in aging rats
Provedor de dados: 56
Autores: Silva,V.J. Dias da; Ferreira Neto,E.; Salgado,H.C.; Fazan Júnior,R.
The aim of the present study was to investigate the effects of converting enzyme inhibition by captopril on ECG parameters in aged rats. Four-month-old male rats received captopril dissolved in tap water (0.5 mg/l) or tap water for 2 or 20 months. At the end of treatment, under anesthesia, RR and PR interval, P wave and QRS duration, QT and corrected QT interval were measured in all animals. On the following day, chronic ECG (lead II) recordings were performed to quantify supraventricular (SVPB) or ventricular premature beats (VPB). After sacrifice, the hearts were removed and weighed. RR interval was similar in young and untreated aged rats, but significantly larger in aged rats treated with captopril. P wave and QRS length did not differ among groups. PR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Electrocardiogram; Captopril; Rats.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900003

Prevalence of deltaF508, G551D, G542X, and R553X mutations among cystic fibrosis patients in the North of Brazil
Provedor de dados: 56
Autores: Araújo,F.G. de; Novaes,F.C.; Santos,N.P.C. dos; Martins,V.C.; Souza,S.M. de; Santos,S.E.B. dos; Ribeiro-dos-Santos,A.K.C.
Cystic fibrosis (CF) is the most common genetic disease among Caucasians and is rare among sub-Saharan Africans. The Brazilian population is not ethnically homogeneous but it is the result of three-way ethnic admixture of Europeans, Africans and Amerindians in varying proportions, depending on the region. In the present study, we investigated 33 patients who had been diagnosed and are currently under treatment for CF at the University Hospital João de Barros Barreto, Belém, Pará State. The molecular analysis for G542X, G551D and R553X mutations was performed by PCR followed by RFLP using BstNI, HincII and MboI, respectively, in polyacrylamide gel eletrophoresis and stained with AgNO3. ThedeltaF508 mutation (a deletion of 3 bp) was only analyzed by...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cystic fibrosis; Gene mutations; Amazon region; Belém; DeltaF508 gene; G551D gene.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100003

Insights into the role of hydration in protein structure and stability obtained through hydrostatic pressure studies
Provedor de dados: 56
Autores: Royer,C.A.
A thorough understanding of protein structure and stability requires that we elucidate the molecular basis for the effects of both temperature and pressure on protein conformational transitions. While temperature effects are relatively well understood and the change in heat capacity upon unfolding has been reasonably well parameterized, the state of understanding of pressure effects is much less advanced. Ultimately, a quantitative parameterization of the volume changes (at the basis of pressure effects) accompanying protein conformational transitions will be required. The present report introduces a qualitative hypothesis based on available model compound data for the molecular basis of volume change upon protein unfolding and its dependence on temperature.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pressure perturbation calorimetry; Folding; Thermal expansivity; Volume change.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800003

Distribution of human immunodeficiency virus type 1 subtypes in the state of Amazonas, Brazil, and subtype C identification
Provedor de dados: 56
Autores: Cunha,L.K.H.; Kashima,S.; Amarante,M.F.C.; Haddad,R.; Rodrigues,E.S.; Silva,K.L.T.; Lima,T.A.; Castro,D.B.; Brito,F.C.; Almeida,E.G.; Covas,D.T.; Malheiro,A.
Few studies have reported the molecular epidemiological characterization of HIV-1 in the Northern region of Brazil. The present study reports the molecular and epidemiological characterization of 31 HIV-1 isolates from blood donors from the State of Amazonas who donated blood between April 2006 and March 2007. Serum/plasma samples from all donors were screened for HIV antibodies by ELISA and the results confirmed by Western blot analysis. Genomic DNA was extracted from the buffy coat using the Super Quik-Gene-DNA Isolation kit. Nested PCR was performed on the env, gag, and pol regions of HIV-1 using the Gene Amp PCR System 9700. Sequencing reactions were performed using the inner PCR primers and the DYEnamic™ ET Dye Terminator Kit, and phylogenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; Subtypes; Phylogenetic analysis; Blood donors; Molecular and epidemiological characterization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200003

Effect of melatonin administration on subjective sleep quality in chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Nunes,D.M.; Mota,R.M.S.; Machado,M.O.; Pereira,E.D.B.; de Bruin,V.M.S.; de Bruin,P.F.C.
Disturbed sleep is common in chronic obstructive pulmonary disease (COPD). Conventional hypnotics worsen nocturnal hypoxemia and, in severe cases, can lead to respiratory failure. Exogenous melatonin has somnogenic properties in normal subjects and can improve sleep in several clinical conditions. This randomized, double-blind, placebo-controlled study was carried out to determine the effects of melatonin on sleep in COPD. Thirty consecutive patients with moderate to very severe COPD were initially recruited for the study. None of the participants had a history of disease exacerbation 4 weeks prior to the study, obstructive sleep apnea, mental disorders, current use of oral steroids, methylxanthines or hypnotic-sedative medication, nocturnal oxygen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic obstructive pulmonary disease; Melatonin; Sleep.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000016

Cardiorespiratory and metabolic determinants during moderate and high resistance exercise intensities until exhaustion using dynamic leg press: comparison with critical load
Provedor de dados: 56
Autores: Arakelian,V.M.; Goulart,C.L.; Mendes,R.G.; Caruso,F.C.; Baldissera,V.; Arena,R.; Borghi-Silva,A.
The objective of this study was to assess cardiovascular, respiratory, and metabolic responses during a commonly used dynamic leg press resistance exercise until exhaustion (TEx) at different intensities and compare with critical load (CL). This was a prospective, cross-sectional, controlled, and crossover study. Twelve healthy young men (23±2.5 years old) participated. The subjects carried out three bouts of resistance exercise in different percentages of 1 repetition maximum (60, 75, and 90% 1RM) until TEx. CL was obtained by means of hyperbolic model and linearization of the load-duration function. During all bout intensities, oxygen uptake (VO2), carbon dioxide production (VCO2), ventilation (VE), and respiratory exchange ratio (RER) were obtained....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Load; Physiological; Muscle fatigue; Exercise; Cardiorespiratory.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100613

Monounsaturated fats and immune function
Provedor de dados: 56
Autores: Yaqoob,P.
Animal studies suggest that olive oil is capable of modulating functions of cells of the immune system in a manner similar to, albeit weaker than, fish oils. There is some evidence that the effects of olive oil on immune function in animal studies are due to oleic acid rather than to trace elements or antioxidants. Importantly, several studies have demonstrated effects of oleic acid-containing diets on in vivo immune responses. In contrast, consumption of a monounsaturated fatty acid (MUFA)-rich diet by humans does not appear to bring about a general suppression of immune cell functions. The effects of this diet in humans are limited to decreasing aspects of adhesion of peripheral blood mononuclear cells, although there are trends towards decreases in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatty acids; Lipids; Immunity; Olive oil; Adhesion molecules.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400001

Participation of hepatic α/β-adrenoceptors and AT1 receptors in glucose release and portal hypertensive response induced by adrenaline or angiotensin II
Provedor de dados: 56
Autores: de Araújo,L.J.T.; Nagaoka,M.R.; Borges,D.R.; Kouyoumdjian,M.
It has been previously demonstrated that the hemodynamic effect induced by angiotensin II (AII) in the liver was completely abolished by losartan while glucose release was partially affected by losartan. Angiotensin II type 1 (AT1) and adrenergic (∝1- and β-) receptors (AR) belong to the G-proteins superfamily, which signaling promote glycogen breakdown and glucose release. Interactive relationship between AR and AT1-R was shown after blockade of these receptors with specific antagonists. The isolated perfused rat liver was used to study hemodynamic and metabolic responses induced by AII and adrenaline (Adr) in the presence of AT1 (losartan) and ∝1-AR and β-AR antagonists (prazosin and propranolol). All antagonists diminished the hemodynamic response...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver perfusion; Angiotensin II; Adrenaline; AT1R; Adrenoceptors.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200604

Association between MCP-1 -2518A&amp;gt;G polymorphism and asthma susceptibility: a meta-analysis
Provedor de dados: 56
Autores: Chen,Wenli; Cui,Jiewei; Xiang,Guoan; Zhang,Jianpeng; Gao,Hongmei.
The published data on the association between MCP-1 -2518A&gt;G polymorphism and asthma susceptibility are inconclusive. Therefore, we performed a meta-analysis to estimate the impact of MCP-1 -2518A&gt;G polymorphism on asthma susceptibility. PubMed, Web of Science, Wanfang, and China National Knowledge Infrastructure (CNKI) databases were used to identify eligible studies. The pooled odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were used to calculate the strength of association. Sensitivity analysis was performed to evaluate the influence of individual studies on the estimates of overall effect, and funnel plots and Egger's test were used to assess publication bias. Eight publications with 1562 asthma patients and 1574 controls were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MCP-1; Polymorphism; Asthma; Susceptibility; Meta-analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100602

Gap effect and reaction time distribution: simple vs choice manual responses
Provedor de dados: 56
Autores: Machado-Pinheiro,W.; Gawryszewski,L.G.; Ribeiro-do-Valle,L.E.
It is well known that saccadic reaction times (SRT) are reduced when the target is preceded by the offset of the fixation point (FP) - the gap effect. Some authors have proposed that the FP offset also allows the saccadic system to generate a separate population of SRT, the express saccades. Nevertheless, there is no agreement as to whether the gap effect and express responses are also present for manual reaction times (MRT). We tested the gap effect and the MRT distribution in two different conditions, i.e., simple and choice MRT. In the choice MRT condition, subjects need to identify the side of the stimulus and to select the appropriate response, while in the simple MRT these stages are not necessary. We report that the gap effect was present in both...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Attention; Reaction time; Gap effect; Vision; Express responses.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000012

Adherence to drug therapy in kidney disease
Provedor de dados: 56
Autores: Magacho,E.J.C.; Ribeiro,L.C.; Chaoubah,A.; Bastos,M.G.
Non-adherence to drug therapy has not been extensively studied in patients with chronic kidney disease (CKD). The objective of the present study was to identify determinants of non-adherence to drug therapy in patients with CKD, not on dialysis. A prospective cohort study involving 149 patients was conducted over a period of 12 months. Adherence to drug therapy was evaluated by the self-report method at baseline and at 12 months. Patients who knew the type of drug(s) and the respective number of prescribed pills in use at the visit preceding the interview were considered to be adherent. Patients with cognitive decline were assessed by interviewing their caregivers. Mean patient age was 51 ± 16.7 years. Male patients predominated (60.4%). Univariate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drug adherence; Chronic kidney disease; Drug therapy; Self-report; Nursing.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300014

Dynamics of chest wall volume regulation during constant work rate exercise in patients with chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Takara,L.S.; Cunha,T.M.; Barbosa,P.; Rodrigues,M.K.; Oliveira,M.F.; Nery,L.E.; Neder,J.A.
This study evaluated the dynamic behavior of total and compartmental chest wall volumes [(V CW) = rib cage (V RC) + abdomen (V AB)] as measured breath-by-breath by optoelectronic plethysmography during constant-load exercise in patients with stable chronic obstructive pulmonary disease. Thirty males (GOLD stages II-III) underwent a cardiopulmonary exercise test to the limit of tolerance (Tlim) at 75% of peak work rate on an electronically braked cycle ergometer. Exercise-induced dynamic hyperinflation was considered to be present when end-expiratory (EE) V CW increased in relation to resting values. There was a noticeable heterogeneity in the patterns of V CW regulation as EEV CW increased non-linearly in 17/30 "hyperinflators" and decreased in 13/30...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exertion; Lung mechanics; COPD; Dyspnea; Exercise test.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200024

Correction
Provedor de dados: 56
Autores: Iwama,A.M.; Andrade,G.N.; Shima,P.; Tanni,S.E.; Godoy,I.; Dourado,V.Z.
Tipo: Info:eu-repo/semantics/article
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300016

The RNA interference revolution
Provedor de dados: 56
Autores: Lenz,G.
The discovery of double-stranded RNA-mediated gene silencing has rapidly led to its use as a method of choice for blocking a gene, and has turned it into one of the most discussed topics in cell biology. Although still in its infancy, the field of RNA interference has already produced a vast array of results, mainly in Caenorhabditis elegans, but recently also in mammalian systems. Micro-RNAs are short hairpins of RNA capable of blocking translation, which are transcribed from genomic DNA and are implicated in several aspects from development to cell signaling. The present review discusses the main methods used for gene silencing in cell culture and animal models, including the selection of target sequences, delivery methods and strategies for a successful...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNAi; SiRNA; MiRNA; Gene silencing; Knockdown.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200003

Effects of centrally acting antihypertensive drugs on the microcirculation of spontaneously hypertensive rats
Provedor de dados: 56
Autores: Estato,V.; Araújo,C.V.; Bousquet,P.; Tibiriçá,E.
We investigated the acute effects of centrally acting antihypertensive drugs on the microcirculation of pentobarbital-anesthetized spontaneously hypertensive rats (SHR). The effects of the sympatho-inhibitory agents clonidine and rilmenidine, known to activate both alpha2-adrenoceptors and nonadrenergic I1-imidazoline binding sites (I1BS) in the central nervous system, were compared to those of dicyclopropylmethyl-(4,5-dimethyl-4,5-dihydro-3H -pyrrol-2-yl)-amine hydrochloride (LNP 509), which selectively binds to the I1BS. Terminal mesenteric arterioles were observed by intravital microscopy. Activation of the central sympathetic system with L-glutamate (125 µg, ic) induced marked vasoconstriction of the mesenteric microcirculation (27 ± 3%; N = 6, P &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenteric microcirculation; Arterial hypertension; Clonidine; Rilmenidine; Baclofen; LNP 509.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000014

An agent-based simulation combined with group decision-making technique for improving the performance of an emergency department
Provedor de dados: 56
Autores: Yousefi,M.; Ferreira,R.P.M.
This study presents an agent-based simulation modeling in an emergency department. In a traditional approach, a supervisor (or a manager) allocates the resources (receptionist, nurses, doctors, etc.) to different sections based on personal experience or by using decision-support tools. In this study, each staff agent took part in the process of allocating resources based on their observation in their respective sections, which gave the system the advantage of utilizing all the available human resources during the workday by being allocated to a different section. In this simulation, unlike previous studies, all staff agents took part in the decision-making process to re-allocate the resources in the emergency department. The simulation modeled the behavior...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agent-based simulation; Patient flow; Decision making; Emergency department; Health industry.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500701

Bilateral repetitive transcranial magnetic stimulation for treatment-resistant depression: a systematic review and meta-analysis of randomized controlled trials
Provedor de dados: 56
Autores: Zhang,Y.Q.; Zhu,D.; Zhou,X.Y.; Liu,Y.Y.; Qin,B.; Ren,G.P.; Xie,P.
There has been concern regarding the use of controversial paradigms for repetitive transcranial magnetic stimulation (rTMS) to manage treatment-resistant depression (TRD). This meta-analysis assessed the efficacy of bilateral rTMS compared with unilateral and sham rTMS in patients with TRD. PubMed, Embase, CENTRAL, PsycINFO, Web of Science, EAGLE and NTIS databases were searched to identify relevant studies, and randomized controlled trials (RCTs) on bilateral rTMS for TRD patients were included. The response was defined as the primary outcome, and remission was the secondary outcome. Ten RCTs that included 634 patients met the eligibility criteria. The risk ratio (RRs) of both the primary and secondary outcomes of bilateral rTMS showed non-significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Repetitive transcranial magnetic stimulation Treatment-resistant depression Meta-analysis; Randomized controlled trials.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300198

Effect of histamine H1 and H2 receptor antagonists, microinjected into cerebellar vermis, on emotional memory consolidation in mice
Provedor de dados: 56
Autores: Gianlorenço,A.C.L.; Serafim,K.R.; Canto-de-Souza,A.; Mattioli,R.
This study investigated the effects of histamine H1 or H2 receptor antagonists on emotional memory consolidation in mice submitted to the elevated plus maze (EPM). The cerebellar vermis of male mice (Swiss albino) was implanted using a cannula guide. Three days after recovery, behavioral tests were performed in the EPM on 2 consecutive days (T1 and T2). Immediately after exposure to the EPM (T1), animals received a microinjection of saline (SAL) or the H1 antagonist chlorpheniramine (CPA; 0.016, 0.052, or 0.16 nmol/0.1 µL) in Experiment 1, and SAL or the H2 antagonist ranitidine (RA; 0.57, 2.85, or 5.7 nmol/0.1 µL) in Experiment 2. Twenty-four hours later, mice were reexposed to the EPM (T2) under the same experimental conditions but they did not receive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorpheniramine; Ranitidine; Cerebellar vermis; Emotional memory consolidation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200135

Binding sites and actions of Tx1, a neurotoxin from the venom of the spider Phoneutria nigriventer, in guinea pig ileum
Provedor de dados: 56
Autores: Santos,R.G.; Diniz,C.R.; Cordeiro,M.N.; De Lima,M.E.
Tx1, a neurotoxin isolated from the venom of the South American spider Phoneutria nigriventer, produces tail elevation, behavioral excitation and spastic paralysis of the hind limbs after intracerebroventricular injection in mice. Since Tx1 contracts isolated guinea pig ileum, we have investigated the effect of this toxin on acetylcholine release, as well as its binding to myenteric plexus-longitudinal muscle membranes from the guinea pig ileum. [125I]-Tx1 binds specifically and with high affinity (Kd = 0.36 ± 0.02 nM) to a single, non-interacting (nH = 1.1), low capacity (Bmax 1.1 pmol/mg protein) binding site. In competition experiments using several compounds (including ion channel ligands), only PhTx2 and PhTx3 competed with [125I]-Tx1 for specific...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Phoneutria nigriventer; Spider toxins; Binding; Enteric nervous system; Calcium channels; Guinea pig ileum.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200019

Spreading depression is facilitated in adult rats previously submitted to short episodes of malnutrition during the lactation period
Provedor de dados: 56
Lactating rat dams were submitted to short episodes (1, 2 or 3 weeks) of nutritional restriction by receiving the "regional basic diet" (RBD, with 8% protein) of low-income human populations of Northeast Brazil. Their pups were then studied regarding the developmental effects on body and brain weights. When the rats reached adulthood, cortical susceptibility to the phenomenon of spreading depression (SD) was evaluated by performing electrophysiological recordings on the surface of the cerebral cortex. SD was elicited at 20-min intervals by applying 2% KCl for 1 min to a site on the frontal cortex and its occurrence was monitored at 2 sites in the parietal region by recording the electrocorticogram and the slow potential change of SD. When compared to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Early protein malnutrition; Spreading depression; Brain growth spurt period.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500015

Wheat bran- but not oat bran-enriched diets increase the mucosal height of the cecum and colon of newly weaned and aged rats
Provedor de dados: 56
Autores: Cavaglieri-Felippe,C.R.; Polacow,M.L.O.; Campos,M.R.; Vecchia,M.G.; Curi,R.
The effect of diets enriched with oat or wheat bran (prepared by the addition of 300 g of each fiber to 1000 g of the regular diet), given for 8 weeks, on the mucosal height of the colon and cecum was investigated. Newly weaned (21 days old) and aged (12 months old) male Wistar rats were used in this study. As compared to controls, diets enriched with wheat bran provoked a significant increase in the mucosal height, whereas oat bran did not cause any effect. In newly weaned rats (21 days old), wheat bran increased the mucosal height (µm) in the cecum by 20% (mean ± SEM for 8 rats; 169.1 ± 5.2 and 202.9 ± 8.0 for control and wheat bran, respectively) and in the colon (218.8 ± 7.2 and 264.5 ± 18.8 for control and wheat bran, respectively). A similar effect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oat bran; Wheat bran; Colon; Cecum; Mucosa; Ageing.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800016

Role of ACTH receptor in adrenocortical tumor formation
Provedor de dados: 56
Autores: Latronico,A.C.
Adrenocorticotrophin (ACTH) is the major regulatory hormone of steroid synthesis and secretion by adrenocortical cells. The actions of ACTH are mediated by its specific membrane receptor (ACTH-R). The human ACTH-R gene was recently cloned, allowing systematic determination of its sequence, expression and function in adrenal tumorigenesis. The presence of oncogenic mutations of the ACTH-R gene in adrenocortical tumors has been reported. Direct sequencing of the entire coding region of the ACTH-R gene of sporadic adrenocortical adenomas and carcinomas did not reveal constitutive activating mutations, indicating that this mechanism is not frequent in human adrenocortical tumorigenesis. Recent studies demonstrated allelic loss of the ACTH-R gene in a subset of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenocorticotrophin receptor; Adrenocortical tumors; Tumorigenesis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000016

Relationship of IL-1 and TNF-α polymorphisms with Helicobacter pylori in gastric diseases in a Brazilian population
Provedor de dados: 56
Autores: Santos,J.C.; Ladeira,M.S.P.; Pedrazzoli Jr,J.; Ribeiro,M.L.
It is well known that the risk of development of gastric cancer (GC) in Helicobacter pylori-infected patients depends on several factors. Thus, the aim of this study was to investigate the effect of proinflammatory cytokine gene polymorphisms for IL-1β, IL-1RN and TNF-α on the development of GC in a Brazilian population. A total of 202 biopsies obtained from Brazilian patients with chronic gastritis and GC were included in the study. Infection with H. pylori cagA+ was determined by the polymerase chain reaction (PCR) as previously described. IL-1β, IL-1RN and TNF-α polymorphism genotyping was performed by restriction fragment length polymorphism PCR. Associations between gene polymorphisms, clinical diseases and virulence markers were evaluated using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic gastritis; Gastric cancer; Polymorphisms; Helicobacter pylori; Interleukins; IL-1β; IL-1RN; TNF-α.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900004

Response of the growth plate of uremic rats to human growth hormone and corticosteroids
Provedor de dados: 56
Autores: Barbosa,A.P.F.; Silva,J.D.P.; Fonseca,E.C.; Lopez,P.M.; Fernandes,M.B.C.; Balduino,A.; Duarte,M.E.L.
Children with chronic renal failure in general present growth retardation that is aggravated by corticosteroids. We describe here the effects of methylprednisolone (MP) and recombinant human growth hormone (rhGH) on the growth plate (GP) of uremic rats. Uremia was induced by subtotal nephrectomy in 30-day-old rats, followed by 20 IU kg-1 day-1 rhGH (N = 7) or 3 mg kg-1 day-1 MP (N = 7) or 20 IU kg-1 day-1 rhGH + 3 mg kg-1 day-1 MP (N = 7) treatment for 10 days. Control rats with intact renal function were sham-operated and treated with 3 mg kg-1 day-1 MP (N = 7) or vehicle (N = 7). Uremic rats (N = 7) were used as untreated control animals. Structural alterations in the GP and the expression of anti-proliferating cell nuclear antigen (PCNA) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corticosteroids; Growth hormone; Renal failure; Growth retardation; Growth plate; Insulin-like growth factor I.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800011

Interleukin-6 contributes to myocardial damage in pregnant rats with reduced uterine perfusion pressure
Provedor de dados: 56
Autores: Ding,Lan; Bai,Chuanming; Liu,Ying.
Preeclampsia is one of the most frequent and difficult illnesses in pregnancy, which jeopardizes both mother and fetus. There are several diagnostic criteria for preeclampsia. However, the preeclampsia-associated myocardial damage has not been described. In this study, we employed reduced uterine perfusion pressure (RUPP) to generate a rat model of preeclampsia for the evaluation of myocardial damage in late-gestation rats. The expressions of cardiac injury markers were analyzed by immunohistochemistry and ELISA. The arterial pressure and myocardial tissue velocities were also measured. The role of interleukin (IL)-6 in RUPP-associated myocardial damage was further explored. The results showed that RUPP rats had significant myocardial damage, as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preeclampsia; Myocardial damage; Reduced uterine perfusion pressure; Interleukin-6.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800608

Contested dominance modifies the anovulatory consequences of social subordination in female marmosets
Provedor de dados: 56
Autores: Alencar,A.I.; Sousa,M.B.C.; Abbott,D.H.; Yamamoto,M.E.
Dominance status among female marmosets is reflected in agonistic behavior and ovarian function. Socially dominant females receive submissive behavior from subordinates, while exhibiting normal ovulatory function. Subordinate females, however, receive agonistic behavior from dominants, while exhibiting reduced or absent ovulatory function. Such disparity in female fertility is not absolute, and groups with two breeding females have been described. The data reported here were obtained from 8 female-female pairs of captive female marmosets, each housed with a single unrelated male. Pairs were classified into two groups: "uncontested" dominance (UD) and "contested" dominance (CD), with 4 pairs each. Dominant females in UD pairs showed significantly higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Callitrichids; Dominance; Progesterone levels; Agonistic behavior; Marmosets.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500012

Effect of felodipine on myocardial and renal injury induced by aldosterone-high salt hypertension in uninephrectomized rats
Provedor de dados: 56
Autores: Matsubara,B.B.; Franco,M.; Janicki,J.S.; Matsubara,L.S.
It has been recently shown that calcium channel blockers might have a protective effect on cardiac fibrogenesis induced by aldosterone. The objective of this study was to evaluate the protective effect of felodipine, a dihydropyridine calcium channel blocker, against heart and kidney damage caused by aldosterone-high sodium intake in uninephrectomized rats. Wistar rats were divided into three groups: CNEP (uninephrectomized + 1% NaCl in the drinking water, N = 9); ALDO (same as CNEP group plus continuous infusion of 0.75 µg/h aldosterone, N = 12); ALDOF (same as ALDO group plus 30 mg·kg-1·day-1 felodipine in the drinking water, N = 10). All results were compared with those of age-matched, untreated rats (CTL group, N = 10). After 6 weeks, tail cuff blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aldosterone; Dihydropyridine; Fibrinoid necrosis; Vascular remodeling; Hypertension; Myocardial remodeling.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500013

Evaluation of chromosomal abnormalities by cIg-FISH and association with proliferative and apoptotic indexes in multiple myeloma
Provedor de dados: 56
Autores: Linardi,C.C.G.; Martinez,G.; Velloso,E.D.R.P.; Leal,A.M.; Kumeda,C.A.; Buccheri,V.; Azevedo,R.S.; Peliçario,L.M.; Dorlhiac-Llacer,P.
Eighty-six newly diagnosed multiple myeloma (MM) patients from a public hospital of São Paulo (Brazil) were evaluated by cIg-FISH for the presence of del(13)(q14), t(4;14)(p16.3;q32) and del(17)(p13). These abnormalities were observed in 46.5, 9.3, and 7.0% of the patients, respectively. In order to identify the possible role of del(13)(q14) in the physiopathology of MM, we investigated the association between this abnormality and the proliferative and apoptotic indexes of plasma cells. When cases demonstrating t(4;14)(p16.3;q32) and del(17)(p13) were excluded from the analysis, we observed a trend towards a positive correlation between the proportion of cells carrying del(13)(q14) and plasma cell proliferation, determined by Ki-67 expression (r = 0.23, P...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Multiple myeloma; Cell proliferation; Apoptosis; Prognosis; Fluorescence in situ hybridization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100012

Diet plus insulin compared to diet alone in the treatment of gestational diabetes mellitus: a systematic review
Provedor de dados: 56
Autores: Giuffrida,F.M.A.; Castro,A.A.; Atallah,A.N.; Dib,S.A.
Fetuses of mothers with gestational diabetes mellitus are at increased risk to develop perinatal complications mainly due to macrosomia. However, in view of the marked heterogeneity of this disease, it seems difficult to set guidelines for diagnosis and treatment. This complicates the choice of assigning patients either to diet or to insulin therapy. Also of concern is how much benefit could be expected from insulin therapy in preventing fetal complications in these patients. In a systematic review of the literature assessing the efficacy of insulin in preventing macrosomia in fetuses of mothers with gestational diabetes, we found six randomized controlled trials comparing diet alone to diet plus insulin. The studies included a total of 1281 patients (644...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diabetes; Gestational; Diet therapy; Fetal macrosomia; Insulin; Meta-analysis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000004

Intima-media thickness evaluation by B-mode ultrasound: Correlation with blood pressure levels and cardiac structures
Provedor de dados: 56
Autores: Plavnik,F.L.; Ajzen,S.; Kohlmann Jr.,O.; Tavares,A.; Zanella,M.T.; Ribeiro,A.B.; Ramos,O.L.
The aim of this study was to analyze the thickness of the intima-media complex (IMC) using a noninvasive method. The carotid and femoral common arteries were evaluated by noninvasive B-mode ultrasound in 63 normotensive and in 52 hypertensive subjects and the thickness of the IMC was tested for correlation with blood pressure, cardiac structures and several clinical and biological parameters. The IMC was thicker in hypertensive than in normotensive subjects (0.67 ± 0.13 and 0.62 ± 0.16 vs 0.54 ± 0.09 and 0.52 ± 0.11 mm, respectively, P&amp;lt;0.0001). In normotensive patients, the simple linear regression showed significant correlations between IMC and age, body mass index and 24-h systolic blood pressure for both the carotid and femoral arteries. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arterial thickness; B-mode ultrasound; 24-ABPM; Cardiac structures.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100008

Prolactin and cortisol levels in women with endometriosis
Provedor de dados: 56
Autores: Lima,A.P.; Moura,M.D.; Rosa e Silva,A.A.M.
Endometriosis is a progressive estrogen-dependent disease affecting women during their reproductive years. The objective of the present study was to investigate whether endometriosis is associated with stress parameters. We determined cortisol and prolactin levels in serum, peritoneal and follicular fluid from infertile women with endometriosis and fertile women without the disease. The extent of the disease was staged according to the revised American Fertility Society classification (1997). Serum and peritoneal fluid were collected from 49 women aged 19 to 39 years undergoing laparoscopy. Eighteen women had stage I-II endometriosis and 10 had stage III-IV. Controls were 21 women undergoing laparoscopy for tubal sterilization. Follicular fluid was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endometriosis; Stress; Infertility; Prolactin; Cortisol.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800015

Attenuation and immunogenicity of recombinant yellow fever 17D-dengue type 2 virus for rhesus monkeys
Provedor de dados: 56
Autores: Galler,R.; Marchevsky,R.S.; Caride,E.; Almeida,L.F.C.; Yamamura,A.M.Y.; Jabor,A.V.; Motta,M.C.A.; Bonaldo,M.C.; Coutinho,E.S.F.; Freire,M.S.
A chimeric yellow fever (YF)-dengue serotype 2 (dengue 2) virus was constructed by replacing the premembrane and envelope genes of the YF 17D virus with those from dengue 2 virus strains of Southeast Asian genotype. The virus grew to high titers in Vero cells and, after passage 2, was used for immunogenicity and attenuation studies in rhesus monkeys. Subcutaneous immunization of naive rhesus monkeys with the 17D-D2 chimeric virus induced a neutralizing antibody response associated with the protection of 6 of 7 monkeys against viremia by wild-type dengue 2 virus. Neutralizing antibody titers to dengue 2 were significantly lower in YF-immune animals than in YF-naive monkeys and protection against challenge with wild-type dengue 2 virus was observed in only 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: YF 17D virus recombinants; Dengue vaccine; Immunogenicity; Attenuation; Rhesus monkeys.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200012

Deficiency of sex hormones does not affect 17-ß-estradiol-induced coronary vasodilation in the isolated rat heart
Provedor de dados: 56
Autores: Santos,R.L.; Lima,J.T.; Rouver,W.N.; Moysés,M.R.
The relaxation of coronary arteries by estrogens in the coronary vascular beds of naive and hypertensive rats has been well described. However, little is known about this action in gonadectomized rats. We investigated the effect of 17-ß-estradiol (E2) in coronary arteries from gonadectomized rats, as well as the contributions of endothelium-derived factors and potassium channels. Eight-week-old female and male Wistar rats weighing 220-300 g were divided into sham-operated and gonadectomized groups (n=9−12 animals per group). The baseline coronary perfusion pressure (CPP) was determined, and the vasoactive effects of 10 μM E2 were assessed by bolus administration before and after endothelium denudation or by perfusion with NG-nitro-L-arginine methyl ester...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gonadectomy; Estrogen; Vasodilation; Coronary arteries.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500603

Streptozotocin-induced diabetes mellitus affects lysosomal enzymes in rat liver
Provedor de dados: 56
Autores: Peres,G.B.; Juliano,M.A.; Aguiar,J.A.K.; Michelacci,Y.M.
It has been previously shown that dextran sulfate administered to diabetic rats accumulates in the liver and kidney, and this could be due to a malfunction of the lysosomal digestive pathway. The aim of the present study was to evaluate the expression and activities of lysosomal enzymes that act upon proteins and sulfated polysaccharides in the livers of diabetic rats. Diabetes mellitus was induced by streptozotocin in 26 male Wistar rats (12 weeks old), while 26 age-matched controls received only vehicle. The livers were removed on either the 10th or the 30th day of the disease, weighed, and used to evaluate the activity, expression, and localization of lysosomal enzymes. A 50-60% decrease in the specific activities of cysteine proteases, especially...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver; Diabetes mellitus; Lysosomal enzymes; Cathepsin; Glycosidase; Sulfatase.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600452

How are antibodies involved in the protective mechanism of susceptible mice infected with T. cruzi?
Provedor de dados: 56
Autores: Umekita,L.F.; Mota,I.
Host resistance to Trypanosoma cruzi is dependent on both natural and acquired immune responses. During the acute phase of the infection the presence of IFN-&lt;FONT FACE=Symbol&gt;g&lt;/font&gt;, TNF-&lt;FONT FACE=Symbol&gt;a&lt;/font&gt;, IL-12 and GM-CSF has been closely associated with resistance, whereas TGF-ß and IL-10 have been associated with susceptibility. Several investigators have demonstrated that antibodies are responsible for the survival of susceptible animals in the initial phase of infection and for the maintenance of low levels of parasitemia in the chronic phase. However, how this occurs is not yet understood. Our results and other data in the literature support the hypothesis that the protective role of antibodies in the acute phase of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Trypanosoma cruzi; Chronic mouse antibodies; Clearance; Cytokines; Acute phase; Susceptible mice.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300001

Improved biological performance of magnesium by micro-arc oxidation
Provedor de dados: 56
Autores: Ma,W.H.; Liu,Y.J.; Wang,W.; Zhang,Y.Z.
Magnesium and its alloys have recently been used in the development of lightweight, biodegradable implant materials. However, the corrosion properties of magnesium limit its clinical application. The purpose of this study was to comprehensively evaluate the degradation behavior and biomechanical properties of magnesium materials treated with micro-arc oxidation (MAO), which is a new promising surface treatment for developing corrosion resistance in magnesium, and to provide a theoretical basis for its further optimization and clinical application. The degradation behavior of MAO-treated magnesium was studied systematically by immersion and electrochemical tests, and its biomechanical performance when exposed to simulated body fluids was evaluated by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnesium; Micro-arc oxidation; Biodegradable; Biocompatibility; Mechanical properties; Corrosion.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300214

The hematopoietic stroma
Provedor de dados: 56
Autores: Nardi,N.B.; Alfonso,Z.Z.C.
All blood cells are derived from a small common pool of totipotent cells, called hematopoietic stem cells. The process is strictly regulated by the hematopoietic microenvironment, which includes stromal cells, extracellular matrix molecules and soluble regulatory factors. Several experimental in vitro assays have been developed for the study of hematopoietic differentiation, and have provided valuable information on the stroma, which includes, among other cell types, macrophages, fibroblasts, adipocytes, and endothelial cells. The composition, ontogeny, and function in physiological as well as pathological conditions of stroma are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematopoiesis; Microenvironment; Stroma.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500014

Oxidative stress and cellular immunity in patients with recurrent aphthous ulcers
Provedor de dados: 56
Autores: Avci,E.; Akarslan,Z.Z.; Erten,H.; Coskun-Cevher,S.
Recurrent aphthous ulcer (RAU) is an inflammatory condition of the oral mucosa characterized by painful, well-circumscribed, single or multiple round or ovoid ulcerations. The exact etiologic factor(s) of these ulcerations are not yet understood. The objective of this study was to evaluate inflammatory processes and free radical metabolism of 25 patients with RAUs compared to 25 healthy controls. The levels of malondialdehyde (MDA) and glutathione (GSH) were determined by high-performance liquid chromatography. Tumor necrosis factor-alpha (TNF-α), interleukin-2 (IL-2), IL-10, and IL-12 were determined by ELISA. Nitric oxide (NO), myeloperoxidase (MPO), total antioxidant status (TAS), and total oxidant status (TOS) levels were measured spectroscopically in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recurrent aphthous ulcer; Oxidative stress; Cytokines.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500355

Pathogenesis of gallbladder adenomyomatosis and its relationship with early-stage gallbladder carcinoma: an overview
Provedor de dados: 56
Autores: Pang,Liwei; Zhang,Yan; Wang,Yuwen; Kong,Jing.
The exact pathogenesis of gallbladder adenomyomatosis is still lacking and some controversies over its diagnosis and treatment exist. Originally recognized as a precancerous lesion, adenomyomatosis is currently recognized by recent studies as a benign alteration of the gallbladder that is often associated with cholecystitis and cholecystolithiasis. Gallbladder carcinoma is an extremely malignant disease with a 5-year survival rate of less than 5%. Therefore, it is important to diagnose, differentiate, and confirm the relationship between adenomyomatosis and early-stage gallbladder carcinoma. However, the early clinical symptoms of adenomyomatosis are extremely similar to those of gallbladder stones and cholecystitis, increasing the difficulty to identify...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallbladder adenomyomatosis; Carcinoma; Rokitansky-Aschoff sinuses; Review.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600201

Locally produced mucosal IgG in chickens immunized with conventional vaccines for Newcastle disease virus
Provedor de dados: 56
Autores: Chimeno Zoth,S.; Gómez,E.; Carrillo,E.; Berinstein,A.
Newcastle disease virus (NDV) is the causative agent of an economically important disease, which affects all species of birds worldwide. Current vaccination programs for NDV include the use of either low-virulent live-virus vaccines or inactivated vaccines to induce protective immunity while producing minimal adverse effects in birds. In order to further characterize the immune response elicited by live virus and inactivated NDV conventional vaccines in chickens, we evaluated the presence of specific antibodies in different secretions and in tissue culture supernatants of immunized birds. To this end, we analyzed all the samples by ELISA, using an indirect assay set up in the laboratory. Specific anti-NDV IgG antibodies were detected in tracheal and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Newcastle disease virus vaccines; Systemic immune response; Mucosal immune response.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400010

In vivo antithrombotic properties of a heparin from the oocyte test cells of the sea squirt Styela plicata(Chordata-Tunicata)
Provedor de dados: 56
Autores: Cardilo-Reis,L.; Cavalcante,M.C.M.; Silveira,C.B.M.; Pavão,M.S.G.
In the ascidian Styela plicata, the oocytes are surrounded by two types of accessory cells named follicle cells and test cells. A heparin-like substance with an anticoagulant activity equivalent to 10% of mammalian heparin and about 5% as potent as the mammalian counterpart for the inhibition of thrombin by antithrombin was isolated from the oocyte test cells. In the present study, we compared the antithrombotic and hemorrhagic effects of sea squirt oocyte test cell heparin with those of porcine heparin in rat models of venous thrombosis and blood loss. Intravenous administration of the oocyte test cell heparin to Wistar rats (both sexes, weighing ~300 g, N = 4 in each group) at a dose of 5.0 mg/kg body weight, which produced a 1.8-fold increase in plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascidian; Heparin; Experimental thrombosis; Antithrombotics; Hemorrhage.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100004

Effect of exercise training on ventilatory efficiency in patients with heart disease: a review
Provedor de dados: 56
Autores: Prado,D.M.L.; Rocco,E.A.; Silva,A.G.; Rocco,D.F.; Pacheco,M.T.; Furlan,V.
The analysis of ventilatory efficiency in cardiopulmonary exercise testing has proven useful for assessing the presence and severity of cardiorespiratory diseases. During exercise, efficient pulmonary gas exchange is characterized by uniform matching of lung ventilation with perfusion. By contrast, mismatching is marked by inefficient pulmonary gas exchange, requiring increased ventilation for a given CO2 production. The etiology of increased and inefficient ventilatory response to exercise in heart disease is multifactorial, involving both peripheral and central mechanisms. Exercise training has been recommended as non-pharmacological treatment for patients with different chronic cardiopulmonary diseases. In this respect, previous studies have reported...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart disease; Ventilatory efficiency; Exercise test; Exercise training.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700301

Association between Toll-like receptor 4 Asp299Gly polymorphism and coronary heart disease susceptibility
Provedor de dados: 56
Autores: Wu,B.W.; Zhu,J.; Shi,H.M.; Jin,B.; Wen,Z.C.
Published data on the association between Toll-like receptor 4 (TLR4) Asp299Gly polymorphism and coronary heart disease (CHD) susceptibility are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. English-language studies were identified by searching PubMed and Embase databases (up to November 2016). All epidemiological studies were regarding Caucasians because no TLR4 Asp/Gly and Gly/Gly genotypes have been detected in Asians. A total of 20 case-control studies involving 14,416 cases and 10,764 controls were included in the meta-analysis. Overall, no significant associations were found between TLR4 Asp299Gly polymorphism and CHD susceptibility in the dominant model (OR=0.89; 95%CI=0.74 to 1.06; P=0.20)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toll-like receptor 4; Polymorphism; Coronary heart disease; Meta-analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900609

Effects of entorhinal cortex lesions on memory in different tasks
Provedor de dados: 56
Lesions of the entorhinal cortex produce retrograde memory impairment in both animals and humans. Here we report the effects of bilateral entorhinal cortex lesions caused by the stereotaxic infusion of N-methyl-D-aspartate (NMDA) in rats at two different moments, before or after the training session, on memory of different tasks: two-way shuttle avoidance, inhibitory avoidance and habituation to an open field. Pre- or post-training entorhinal cortex lesions caused an impairment of performance in the shuttle avoidance task, which agrees with the previously described role of this area in the processing of memories acquired in successive sessions. In the inhibitory avoidance task, only the post-training lesions had an effect (amnesia). No effect was observed...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Entorhinal cortex; Memory; NMDA lesions.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600011

Effect of the 5-HT4 receptor and serotonin transporter on visceral hypersensitivity in rats
Provedor de dados: 56
Autores: Yan,Chi; Xin-Guang,Liu; Hua-Hong,Wang; Jun-Xia,Li; Yi-Xuan,Li.
Visceral hypersensitivity plays an important role in motor and sensory abnormalities associated with irritable bowel syndrome, but the underlying mechanisms are not fully understood. The present study was designed to evaluate the expression of the 5-HT4 receptor and the serotonin transporter (SERT) as well as their roles in chronic visceral hypersensitivity using a rat model. Neonatal male Sprague-Dawley rats received intracolonic injections of 0.5% acetic acid (0.3-0.5 mL at different times) between postnatal days 8 and 21 to establish an animal model of visceral hypersensitivity. On day 43, the threshold intensity for a visually identifiable contraction of the abdominal wall and body arching were recorded during rectal distention. Histological evaluation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5-Hydroxytryptamine receptor; Serotonin; Serotonin transport protein; Visceral hypersensitivity; SERT expression.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000010

Central release of nitric oxide mediates antinociception induced by aerobic exercise
Provedor de dados: 56
Autores: Galdino,G.S.; Duarte,I.D.; Perez,A.C.
Nitric oxide (NO) is a soluble gas that participates in important functions of the central nervous system, such as cognitive function, maintenance of synaptic plasticity for the control of sleep, appetite, body temperature, neurosecretion, and antinociception. Furthermore, during exercise large amounts of NO are released that contribute to maintaining body homeostasis. Besides NO production, physical exercise has been shown to induce antinociception. Thus, the present study aimed to investigate the central involvement of NO in exercise-induced antinociception. In both mechanical and thermal nociceptive tests, central [intrathecal (it) and intracerebroventricular (icv)] pretreatment with inhibitors of the NO/cGMP/KATP pathway (L-NOArg, ODQ, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Exercise; Pain; Antinociception.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900790

Exosomes promote cetuximab resistance via the PTEN/Akt pathway in colon cancer cells
Provedor de dados: 56
Autores: Zhang,S.; Zhang,Y.; Qu,J.; Che,X.; Fan,Y.; Hou,K.; Guo,T.; Deng,G.; Song,N.; Li,C.; Wan,X.; Qu,X.; Liu,Y.
Cetuximab is widely used in patients with metastatic colon cancer expressing wildtype KRAS. However, acquired drug resistance limits its clinical efficacy. Exosomes are nanosized vesicles secreted by various cell types. Tumor cell-derived exosomes participate in many biological processes, including tumor invasion, metastasis, and drug resistance. In this study, exosomes derived from cetuximab-resistant RKO colon cancer cells induced cetuximab resistance in cetuximab-sensitive Caco-2 cells. Meanwhile, exosomes from RKO and Caco-2 cells showed different levels of phosphatase and tensin homolog (PTEN) and phosphor-Akt. Furthermore, reduced PTEN and increased phosphorylated Akt levels were found in Caco-2 cells after exposure to RKO cell-derived exosomes....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cetuximab; Exosome; PTEN; Akt; Colon cancer.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100609

Male gonadal function, prolactin secretion and lactotroph population in an experimental model of cirrhosis
Provedor de dados: 56
Autores: Gonzales,P.H.; Rhoden,C.R.; Luz,C.; Corrêa,G.; Barbosa-Coutinho,L.M.; Oliveira,M.C.
Liver cirrhosis, a highly prevalent chronic disease, is frequently associated with endocrine dysfunctions, notably in the gonadal axis. We evaluated lactotroph population by immunohistochemistry, gonadotropins and prolactin by immunoradiometric assay and testosterone and estradiol by radioimmunoassay in adult male Wistar rats with cirrhosis induced by carbon tetrachloride. No significant difference in mean ± SEM percentages of lactotrophs was found between cirrhotic animals and controls (N = 12, mean 18.95 ± 1.29%). Although there was no significant difference between groups in mean serum levels of prolactin (control: 19.2 ± 4 ng/mL), luteinizing hormone (control: 1.58 ± 0.43 ng/mL), follicle-stimulating hormone (control: 19.11 ± 2.28 ng/mL), estradiol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypogonadism; Hyperestrogenemia; Hyperprolactinemia; Lactotrophs; Liver cirrhosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000011

Manual and semi-automatic quantification of in vivo ¹H-MRS data for the classification of human primary brain tumors
Provedor de dados: 56
Autores: Cuellar-Baena,S.; Morais,L.M.T.S.; Cendes,F.; Faria,A.V.; Castellano,G.
In vivo proton magnetic resonance spectroscopy (¹H-MRS) is a technique capable of assessing biochemical content and pathways in normal and pathological tissue. In the brain, ¹H-MRS complements the information given by magnetic resonance images. The main goal of the present study was to assess the accuracy of ¹H-MRS for the classification of brain tumors in a pilot study comparing results obtained by manual and semi-automatic quantification of metabolites. In vivo single-voxel ¹H-MRS was performed in 24 control subjects and 26 patients with brain neoplasms that included meningiomas, high-grade neuroglial tumors and pilocytic astrocytomas. Seven metabolite groups (lactate, lipids, N-acetyl-aspartate, glutamate and glutamine group, total creatine, total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proton magnetic resonance spectroscopy; Brain tumors; AMARES; Tumor classification.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400012

Modulation of B lymphocyte function by an aqueous fraction of the ethanol extract of Cissampelos sympodialis Eichl (Menispermaceae)
Provedor de dados: 56
Autores: Alexandre-Moreira,M.S.; Piuvezam,M.R.; Pecanha,L.M.T.
Cissampelos sympodialis Eichl species are used in folk medicine for the treatment of asthma, arthritis and rheumatism. In the present study, we investigated the immunomodulatory effect of an aqueous fraction of a 70% (v/v) ethanol extract of C. sympodialis leaves on B lymphocyte function. The hydroalcoholic extract inhibited the in vitro proliferative response of resting B cells induced by LPS (IC50 = 17.2 µg/ml), anti-delta-dextran (IC50 = 13.9 µg/ml) and anti-IgM (IC50 = 24.3 µg/ml) but did not affect the anti-MHC class II antibody-stimulated proliferative response of B cell blasts obtained by stimulation with IL-4 and anti-IgM. Incubation with the hydroalcoholic extract used at 50 µg/ml induced a 700% increase in intracellular cAMP levels. IgM secretion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B lymphocytes; Cissampelos sympodialis; Natural products; Trypanosoma cruzi; Immunoglobulin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100010

Cloning, purification and comparative characterization of two digestive lysozymes from Musca domestica larvae
Provedor de dados: 56
Autores: Cançado,F.C.; Chimoy Effio,P; Terra,W.R.; Marana,S.R.
cDNA coding for two digestive lysozymes (MdL1 and MdL2) of the Musca domestica housefly was cloned and sequenced. MdL2 is a novel minor lysozyme, whereas MdL1 is the major lysozyme thus far purified from M. domestica midgut. MdL1 and MdL2 were expressed as recombinant proteins in Pichia pastoris, purified and characterized. The lytic activities of MdL1 and MdL2 upon Micrococcus lysodeikticus have an acidic pH optimum (4.8) at low ionic strength (μ = 0.02), which shifts towards an even more acidic value, pH 3.8, at a high ionic strength (μ = 0.2). However, the pH optimum of their activities upon 4-methylumbelliferyl N-acetylchitotrioside (4.9) is not affected by ionic strength. These results suggest that the acidic pH optimum is an intrinsic property of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lysozyme; Digestive lysozyme; Substrate affinity; PH optimum.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100005

Importance of hyaluronan biosynthesis and degradation in cell differentiation and tumor formation
Provedor de dados: 56
Autores: Heldin,P.
Hyaluronan is an important connective tissue glycosaminoglycan. Elevated hyaluronan biosynthesis is a common feature during tissue remodeling under both physiological and pathological conditions. Through its interactions with hyaladherins, hyaluronan affects several cellular functions such as cell migration and differentiation. The activities of hyaluronan-synthesizing and -degrading enzymes have been shown to be regulated in response to growth factors. During tumor progression hyaluronan stimulates tumor cell growth and invasiveness. Thus, elucidation of the molecular mechanisms which regulate the activities of hyaluronan-synthesizing and -degrading enzymes during tumor progression is highly desired.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyaluronan; Synthase; Hyaluronidase; Tumor invasion; Extracellular matrix; Pericellular matrix.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800002

Anti-Leishmania titers and positive skin tests in patients cured of kala-azar
Provedor de dados: 56
Autores: Viana,G.M.C.; Nascimento,M.D.S.B.; Diniz Neto,J.A.; Rabelo,E.M.F.; Binda Júnior,J.R.; Santos Júnior,O.M.; Santos,A.C.; Galvão,C.S.; Guimarães,R.S.
Visceral leishmaniasis (VL), also known as kala-azar, is an important public health problem. If not treated, virtually all clinically symptomatic patients die within months. The diagnosis is based on the Montenegro skin test (MST) and anti-Leishmania titers. Nevertheless, the time required for cured individuals living in a leishmaniasis-endemic area to present a positive skin test and negative anti-Leishmania serology is known. To determine the cellular and humoral immune response profile in relation to different times post-VL cure, a cross-sectional study was conducted on subjects from a kala-azar endemic area in Paço do Lumiar, MA, Brazil, on the basis of 1995-2005 notifications reported by the National Health Foundation/Regional Coordination of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Human visceral leishmaniasis; Montenegro’s intradermal reaction; Indirect immunofluorescence.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100009

Ginsenoside Rg1 protects human renal tubular epithelial cells from lipopolysaccharide-induced apoptosis and inflammation damage
Provedor de dados: 56
Autores: Ni,X.J.; Xu,Z.Q.; Jin,H.; Zheng,S.L.; Cai,Y.; Wang,J.J.
Ginsenoside Rg1, one of the most notable active components of Panax ginseng, has been widely reported to exert anti-inflammatory actions. This study aimed to reveal whether ginsenoside Rg1 also exhibits beneficial roles against lipopolysaccharide (LPS)-induced apoptosis and inflammation in human renal tubular epithelial cells, and to evaluate the potential role of the component on tubulointerstitial nephritis treatment. HK-2 cells were treated with various doses of ginsenoside Rg1 (0, 50, 100, 150, and 200 μM) in the absence or presence of 5 μg/mL LPS. Thereafter, CCK-8 assay, flow cytometry, western blot, migration assay, reactive oxygen species (ROS) assay, and ELISA were carried out to respectively assess cell viability, apoptosis, migration, ROS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginsenoside Rg1; LPS; Apoptosis; Inflammation; PI3K/AKT pathway; NF-κB pathway.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200608

Relationship between uric acid levels and risk of chronic kidney disease in a retrospective cohort of Brazilian workers
Provedor de dados: 56
Autores: Chini,L.S.N.; Assis,L.I.S.; Lugon,J.R.
Uric acid (UA) levels are increased in patients with kidney dysfunction. We analyzed the association between asymptomatic hyperuricemia and new-onset chronic kidney disease (CKD). A retrospective cohort study was designed to collect data from employees of an energy generation and distribution company in the city of Rio de Janeiro, Brazil, who had undergone the company’s annual medical checkup from 2008 to 2014. People with ≤2 years of follow-up, with baseline estimated glomerular filtration rate (eGFR) &lt;60 mL·min-1·(1.73 m2)-1 or with incomplete data were excluded. The endpoint was defined as eGFR &lt;60 mL·min-1·(1.73 m2)-1 estimated through the chronic kidney disease epidemiology collaboration equation (CKD-EPI). The study included 1094 participants....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic kidney disease; Hyperuricemia; Glomerular filtration rate.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900611

Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine model
Provedor de dados: 56
Autores: Sampaio,I.C.; Medeiros,P.H.Q.S.; Rodrigues,F.A.P.; Cavalcante,P.A.; Ribeiro,S.A.; Oliveira,J.S.; Prata,M.M.G.; Costa,D.V.S.; Fonseca,S.G.C.; Guedes,M.M.; Soares,A.M.; Brito,G.A.C.; Havt,A.; Moore,S.R.; Lima,A.A.M.
Undernutrition represents a major public health challenge for middle- and low-income countries. This study aimed to evaluate whether a multideficient Northeast Brazil regional basic diet (RBD) induces acute morphological and functional changes in the ileum of mice. Swiss mice (∼25 g) were allocated into two groups: i) control mice were fed a standard diet and II) undernourished mice were fed the RBD. After 7 days, mice were killed and the ileum collected for evaluation of electrophysiological parameters (Ussing chambers), transcription (RT-qPCR) and protein expression (western blotting) of intestinal transporters and tight junctions. Body weight gain was significantly decreased in the undernourished group, which also showed decreased crypt depth but no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Undernutrition; Ion transport; Intestinal absorption.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000607

Comparative study of human exposure to mercury in riverside communities in the Amazon region
Provedor de dados: 56
Autores: Pinheiro,M.C.N.; Oikawa,T.; Vieira,J.L.F.; Gomes,M.S.V.; Guimarães,G.A.; Crespo-López,M.E.; Müller,R.C.S.; Amoras,W.W.; Ribeiro,D.R.G.; Rodrigues,A.R.; Côrtes,M.I.T.
Four populations in the Amazon area were selected for a comparative study of mercury-exposed and non-exposed populations: São Luiz do Tapajós, Barreiras, Panacauera, and Pindobal Grande. The highest mercury levels in human hair samples were found in São Luiz do Tapajós and Barreiras, greatly exceeding the limits established by the World Health Organization. Panacauera showed an intermediate level below 9 µg/g. This was the first comparative and simultaneous evaluation of mercury exposure in the Amazon area. Also, thanks to this type of monitoring, we were able to eliminate the uncertainties about the reference dose. On the basis of these data, we can conclude that the mercury levels detected in exposed populations of the Tapajós River basin may be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury; Amazon; Comparative study; Riverside populations; Human exposure; Reference dose.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300012

Proteomic data show an increase in autoantibodies and alpha-fetoprotein and a decrease in apolipoprotein A-II with time in sera from senescence-accelerated mice
Provedor de dados: 56
Autores: Guo,S.J.; Qi,C.H.; Zhou,W.X.; Zhang,Y.X.; Zhang,X.M.; Wang,J.; Wang,H.X.
We evaluated changes in levels by comparing serum proteins in senescence-accelerated mouse-prone 8 (SAMP8) mice at 2, 6, 12, and 15 months of age (SAMP8-2 m, -6 m, -12 m, -15 m) to age-matched SAM-resistant 1 (SAMR1) mice. Mice were sacrificed, and blood was analyzed by 2-dimensional electrophoresis combined with mass spectrometry. Five protein spots were present in all SAMP8 serum samples, but only appeared in SAMR1 samples at 15 months of age except for spot 3, which also showed a slight expression in SAMR1-12 m sera. Two proteins decreased in the sera from SAMP8-2 m, -6 m, and -12 m mice, and divided into 2 spots each in SAMP8-15 m sera. Thus, the total number of altered spots in SAMP8 sera was 7; of these, 4 were identified as Ig kappa chain V region...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SAMP8; Serum proteins; 2-DE; Mass spectrometry; M-T413.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500417

Volatile cardioplegia: fast normothermic cardiac arrest induction and recovery with halothane in isolated rat hearts
Provedor de dados: 56
To study the effect of halothane as a cardioplegic agent, ten Wistar rats were anesthetized by ether inhalation and their hearts were perfused in a Langendorff system with Krebs-Henseleit solution (36oC; 90 cm H2O pressure). After a 15-min period for stabilization the control values for heart rate, force (T), dT/dt and coronary flow were recorded and a halothane-enriched solution (same temperature and pressure) was perfused until cardiac arrest was obtained. The same Krebs-Henseleit solution was reperfused again and the parameters studied were recorded after 1, 3, 5, 10, 20 and 30 min. Cardiac arrest occurred in all hearts during the first two min of perfusion with halothane-bubbled solution. One minute after reperfusion without halothane, the following...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cardioplegia; Myocardial; Protection; Halothane.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600009

Anterograde effects of a single electroconvulsive shock on inhibitory avoidance and on cued fear conditioning
Provedor de dados: 56
Autores: Oliveira,M.G.M.; Bueno,O.F.A.; Gugliano,E.B.
A single electroconvulsive shock (ECS) or a sham ECS was administered to male 3-4-month-old Wistar rats 1, 2, and 4 h before training in an inhibitory avoidance test and in cued classical fear conditioning (measured by means of freezing time in a new environment). ECS impaired inhibitory avoidance at all times and, at 1 or 2 h before training, reduced freezing time before and after re-presentation of the ECS. These results are interpreted as a transient conditioned stimulus (CS)-induced anxiolytic or analgesic effect lasting about 2 h after a single treatment, in addition to the known amnesic effect of the stimulus. This suggests that the effect of anterograde learning impairment is demonstrated unequivocally only when the analgesic/anxiolytic effect is...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Electroconvulsive shock; Memory; Cued fear conditioning; Inhibitory avoidance; Anxiolytic effect; Analgesic effect.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800009

Investigation of modified platelet-rich plasma (mPRP) in promoting the proliferation and differentiation of dental pulp stem cells from deciduous teeth
Provedor de dados: 56
Autores: Wen,J.; Li,H.T.; Li,S.H.; Li,X.; Duan,J.M.
Stem cells from human exfoliated deciduous teeth (SHEDs) have great potential to treat various dental-related diseases in regenerative medicine. They are usually maintained with 10% fetal bovine serum (FBS) in vitro. Modified platelet-rich plasma (mPRP) would be a safe alternative to 10% FBS during SHEDs culture. Therefore, our study aimed to compare the proliferation and differentiation of SHEDs cultured in mPRP and FBS medium to explore an optimal concentration of mPRP for SHEDs maintenance. Platelets were harvested by automatic blood cell analyzer and activated by repeated liquid nitrogen freezing and thawing. The platelet-related cytokines were examined and analyzed by ELISA. SHEDs were extracted and cultured with different concentrations of mPRP or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MPRP; SHEDs; ALP; RUNX2; OCN.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000604

Immediate effects of submaximal effort on pulse wave velocity in patients with Marfan syndrome
Provedor de dados: 56
Autores: Peres,P.; Bernardelli,G.F.; Mendes,C.C.; Fischer,S.S.C.P.M.; Servantes,D.M.; Medeiros,W.M.; Carvalho,A.C.
Marfan syndrome (MS) is a dominant autosomal disease caused by mutations in chromosome 15, the locus controlling fibrillin 1 synthesis, and may exhibit skeletal, ocular, cardiovascular, and other manifestations. Pulse wave velocity (PWV) is used to measure arterial elasticity and stiffness and is related to the elastic properties of the vascular wall. Since the practice of exercise is limited in MS patients, it was of interest to analyze the acute effect of submaximal exercise on aortic distensibility using PWV and other hemodynamic variables in patients with MS with either mild or no aortic dilatation. PWV and physiological variables were evaluated before and after submaximal exercise in 33 patients with MS and 18 controls. PWV was 8.51 ± 0.58 at rest and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise; Aortic elasticity; Pulse wave velocity; Marfan syndrome.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400011

Laboratory evidence for the hematopoietic potential of Beta vulgaris leaf and stalk extract in a phenylhydrazine model of anemia
Provedor de dados: 56
Autores: Gheith,I.; El-Mahmoudy,A.
This study was designed to provide laboratory evidence supporting the hematopoietic effect of Beta vulgaris (beet) leaf aqueous extract in phenylhydrazine-induced anemia model in albino rats. Extraction of the leaves/stalks was done by maceration in 30% hydro-ethanol for 48 h. An intraperitoneal injection of 20 mg/kg phenylhydrazine was applied for two consecutive days to develop hemolytic anemia on the 4th day after the 1st injection in 24 of 30 male albino rats. The animals were divided into 5 groups and received the following treatments: standard (ferrous ascorbate + folic acid; 13.5 + 0.135 mg/kg), B. vulgaris extract (100 and 200 mg/kg), or left untreated (normal and diseased controls). Blood samples were taken at 0, 4, 8, and 12 days of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anemia; Beta vulgaris; Beet; Hematinic; Anti-anemic; Antioxidant.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100611

Human papillomavirus infection among sexual partners attending a Sexually Transmitted Disease Clinic in Rio de Janeiro, Brazil
Provedor de dados: 56
Autores: Afonso,L.A.; Rocha,W.M.; Carestiato,F.N.; Dobao,E.A.; Pesca,L.F.; Passos,M.R.L.; Cavalcanti,S.M.B.
Cervical cancer is a major source of illness and death among women worldwide and genital infection with oncogenic human papillomavirus (HPV) its principal cause. There is evidence of the influence of the male factor in the development of cervical neoplasia. Nevertheless, the pathogenic processes of HPV in men are still poorly understood. It has been observed that different HPV types can be found among couples. The objective of the present study was to investigate HPV infections in female patients (n = 60 females/group) as well as in their sexual partners and to identify the concordance of HPV genotypes among them. By using the polymerase chain reaction, we detected a 95% prevalence of HPV DNA in women with cervical intraepithelial neoplasia (CIN) compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Neoplasia; Couples; Sexual partners; Polymerase chain reaction.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600533

Cardiovascular actions of angiotensin-(1-7)
Provedor de dados: 56
Autores: Ferreira,A.J.; Santos,R.A.S.
Angiotensin-(1-7) (Ang-(1-7)) is now considered to be a biologically active member of the renin-angiotensin system. The functions of Ang-(1-7) are often opposite to those attributed to the main effector component of the renin-angiotensin system, Ang II. Chronic administration of angiotensin-converting enzyme inhibitors (ACEI) increases 10- to 25-fold the plasma levels of this peptide, suggesting that part of the beneficial effects of ACEI could be mediated by Ang-(1-7). Ang-(1-7) can be formed from Ang II or directly from Ang I. Other enzymatic pathways for Ang-(1-7) generation have been recently described involving the novel ACE homologue ACE2. This enzyme can form Ang-(1-7) from Ang II or less efficiently by the hydrolysis of Ang I to Ang-(1-9) with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin-(1-7) receptor Mas; Renin-angiotensin system; Cardiac function; Blood vessels; Angiotensin-converting enzyme 2; Transgenic models.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400003

Influence of transcutaneous electrical stimulation on heterotopic ossification: an experimental study in Wistar rats
Provedor de dados: 56
Autores: Zotz,T.G.G.; Paula,J.B. de.
Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues, resulting in joint mobility deficit and pain. Different treatment modalities have been tried to prevent HO development, but there is no consensus on a therapeutic approach. Since electrical stimulation is a widely used resource in physiotherapy practice to stimulate joint mobility, with analgesic and anti-inflammatory effects, its usefulness for HO treatment was investigated. We aimed to identify the influence of electrical stimulation on induced HO in Wistar rats. Thirty-six male rats (350-390 g) were used, and all animals were anesthetized for blood sampling before HO induction, to quantify the serum alkaline phosphatase. HO induction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heterotopic ossification; Experimental model; Electrical stimulation; Functional electrical stimulation; Transcutaneous electrical nerve stimulation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101055

Restless leg syndrome, sleep quality and fatigue in multiple sclerosis patients
Provedor de dados: 56
Autores: Moreira,N.C.V.; Damasceno,R.S.; Medeiros,C.A.M.; de Bruin,P.F.C.; Teixeira,C.A.C.; Horta,W.G.; de Bruin,V.M.S.
We have tested the hypothesis that restless leg syndrome (RLS) is related to quality of sleep, fatigue and clinical disability in multiple sclerosis (MS). The diagnosis of RLS used the four minimum criteria defined by the International Restless Legs Syndrome Study Group. Fatigue was assessed by the Fatigue Severity Scale (FSS &gt;27), quality of sleep by the Pittsburgh Sleep Quality Index (PSQI &gt;6), excessive daytime sleepiness by the Epworth Sleepiness Scale (ESS &gt;10) and clinical disability by the Expanded Disability Status Scale (EDSS). Forty-four patients (32 women) aged 14 to 64 years (43 ± 14) with disease from 0.4 to 23 years (6.7 ± 5.9) were evaluated. Thirty-five were classified as relapsing-remitting, 5 as primary progressive and 4 as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Restless leg syndrome; Multiple sclerosis; Sleep; Fatigue; Pittsburgh Sleep Quality Index.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000017

Preparation and cytotoxicity of cisplatin-containing liposomes
Provedor de dados: 56
Autores: Carvalho Júnior,A.D.; Vieira,F.P.; De Melo,V.J.; Lopes,M.T.P.; Silveira,J.N.; Ramaldes,G.A.; Garnier-Suillerot,A.; Pereira-Maia,E.C.; De Oliveira,M.C.
We encapsulated cisplatin into stealth pH-sensitive liposomes and studied their stability, cytotoxicity and accumulation in a human small-cell lung carcinoma cell line (GLC4) and its resistant subline (GLC4/CDDP). Since reduced cellular drug accumulation has been shown to be the main mechanism responsible for resistance in the GLC4/CDDP subline, we evaluated the ability of this new delivery system to improve cellular uptake. The liposomes were composed of dioleoylphosphatidylethanolamine (DOPE), cholesteryl hemisuccinate (CHEMS), and distearoylphosphatidylethanolamine-polyethyleneglycol 2000 (DSPE-PEG2000) and were characterized by determining the encapsulation percentage as a function of lipid concentration. Among the different formulations,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cisplatin; Liposomes; Encapsulation; Cytotoxicity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800017

Poly-DL-lactide-co-glycolide microspheres as a controlled release antigen delivery system
Provedor de dados: 56
Autores: Lima,K.M.; Rodrigues Júnior,J.M.
Successful vaccine application means maximum protection with minimal number of administrations. A rational development of vaccines involves studies of the nature of the antigen as well as of the adjuvant to be used to improve the immune responses. This has provided the impetus for studies to design the degradable devices and for different approaches to antigen delivery by different routes of administration. The development of controlled release systems based on polymeric devices that permit a sustained or pulsed release of encapsulated antigens has attracted much interest. Polymeric delivery systems consist of polymers that release their content continuously in a controlled manner over a period of time. The development of a biocompatible delivery system...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaccine; Adjuvant; Microspheres; Microencapsulation; Biomaterial.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200005

Does whole body vibration exercise improve oxidative stress markers in women with fibromyalgia?
Provedor de dados: 56
Autores: Santos,J.M.; Mendonça,V.A.; Ribeiro,V.G.C.; Tossige-Gomes,R.; Fonseca,S.F.; Prates,A.C.N.; Flor,J.; Oliveira,A.C.C.; Martins,J.B.; Garcia,B.C.C.; Leite,H.R.; Figueiredo,P.H.S.; Bernardo-Filho,M.; Lacerda,A.C.R.
The objective of this study was to investigate the effect of whole body vibration (WBV) exercise on oxidative stress markers in a group of women with fibromyalgia (FM) compared to a group of healthy women (CT). Twenty-one women diagnosed with FM and 21 age- and weight-matched healthy women were enrolled the study. Plasma oxidative stress markers (primary outcomes) were evaluated at rest and after WBV, and included thiobarbituric acid reactive substances (TBARS), iron reduction capacity (FRAP), superoxide dismutase antioxidant enzymes activity (SOD), and catalase (CAT). At rest, the FM group had higher TBARS (P&lt;0.001) and FRAP (P&lt;0.001), and lower CAT (P=0.005) compared to the CT. In the CT group, the WBV had no effect on TBARS (P=0.559) and FRAP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Whole body vibration; Chronic disease; Oxidative stress; Fibromyalgia.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800609

Effects of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) release from polylactide-poly (ethylene glycol)-polylactide (PELA) microcapsule-based scaffolds on bone
Provedor de dados: 56
Autores: Ren,Q.; Cai,M.; Zhang,K.; Ren,W.; Su,Z.; Yang,T.; Sun,T.; Wang,J.
Multiple growth factors can be administered to mimic the natural process of bone healing in bone tissue engineering. We investigated the effects of sequential release of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) from polylactide-poly (ethylene glycol)-polylactide (PELA) microcapsule-based scaffolds on bone regeneration. To improve the double emulsion/solvent evaporation technique, VEGF was encapsulated in PELA microcapsules, to which BMP-2 was attached. The scaffold (BMP-2/PELA/VEGF) was then fused to these microcapsules using the dichloromethane vapor method. The bioactivity of the released BMP-2 and VEGF was then quantified in rat mesenchymal stem cells (rMSCs). Immunoblotting analysis showed that BMP-2/PELA/VEG...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone tissue engineering; BMP-2; VEGF; PELA; Scaffold.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200603

Differential regulation of vitamin D receptor expression in distinct leukemic cell lines upon phorbol ester-induced growth arrest
Provedor de dados: 56
Autores: Folgueira,M.A.A.K.; Federico,M.H.H.; Roela,R.A.; Maistro,S.; Katayama,M.L.H.; Brentani,M.M.
A close correlation between vitamin D receptor (VDR) abundance and cell proliferation rate has been shown in NIH-3T3 fibroblasts, MCF-7 breast cancer and in HL-60 myeloblastic cells. We have now determined if this association occurs in other leukemic cell lines, U937 and K562, and if VDR content is related to c-myc expression, which is also linked to cell growth state. Upon phorbol myristate acetate (PMA) treatment, cells from the three lineages (HL-60, U937 and K562) differentiated and expressed specific surface antigens. All cell lines analyzed were growth inhibited by PMA and the doubling time was increased, mainly due to an increased fraction of cells in the G0/G1 phase, as determined by flow cytometry measurements of incorporated bromodeoxyuridine and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Receptors; Calcitriol; Cell division; Cell differentiation; Leukemia; Phorbol esters.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500011

Behavioral effects of endogenous or exogenous estradiol and progesterone on cocaine sensitization in female rats
Provedor de dados: 56
Autores: Souza,M.F.; Couto-Pereira,N.S.; Freese,L.; Costa,P.A.; Caletti,G.; Bisognin,K.M.; Nin,M.S.; Gomez,R.; Barros,H.M.T.
Cocaine sensitization is a marker for some facets of addiction, is greater in female rats, and may be influenced by their sex hormones. We compared the modulatory effects of endogenous or exogenous estradiol and progesterone on cocaine-induced behavioral sensitization in 106 female rats. Ovariectomized female rats received progesterone (0.5 mg/mL), estradiol (0.05 mg/mL), progesterone plus estradiol, or the oil vehicle. Sham-operated control females received oil. Control and acute subgroups received injections of saline, while the repeated group received cocaine (15 mg/kg, ip) for 8 days. After 10 days, the acute and repeated groups received a challenge dose of cocaine, after which locomotion and stereotypy were monitored. The estrous cycle phase was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocaine; Locomotor activity; Female rats; Estradiol; Progesterone; Stereotypic activity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600505

High baseline serum total and LDL cholesterol levels are associated with MDR1 haplotypes in Brazilian hypercholesterolemic individuals of European descent
Provedor de dados: 56
Autores: Rodrigues,A.C.; Rebecchi,I.M.M.; Bertolami,M.C.; Faludi,A.A.; Hirata,M.H.; Hirata,R.D.C.
The MDR1 gene encodes the P-glycoprotein, an efflux transporter with broad substrate specificity. P-glycoprotein has raised great interest in pharmacogenetics because it transports a variety of structurally divergent drugs, including lipid-lowering drugs. The synonymous single-nucleotide polymorphism C3435T and the nonsynonymous single-nucleotide polymorphism G2677T/A in MDR1 have been indicated as potential determinants of variability in drug disposition and efficacy. In order to evaluate the effect of G2677T/A and C3435T MDR1 polymorphisms on serum levels of lipids before and after atorvastatin administration, 69 unrelated hypercholesterolemic individuals from São Paulo city, Brazil, were selected and treated with 10 mg atorvastatin orally once daily for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MDR1 gene; Hypercholesterolemia; Statins; Single nucleotide polymorphism; Pharmacogenetics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900014

Safety and efficacy of regional citrate anticoagulation in continuous blood purification treatment of patients with multiple organ dysfunction syndrome
Provedor de dados: 56
Autores: Tuerdi,B.; Zuo,L.; Sun,H.; Wang,K.; Wang,Z.; Li,G.
The aim of this study was to discuss the safety and efficacy of regional citrate anticoagulation (RCA) on continuous blood purification (CBP) during the treatment of multiple organ dysfunction syndrome (MODS). Thirty-five patients with MODS were divided into two groups: the local citrate anticoagulation (RCA) group, and the heparin-free blood purification (hfBP) group. The MODS severity was assessed according to Marshall’s MODS score criteria. Blood coagulation indicators, blood pressure, filter lifespan, filter replacement frequency, anticoagulation indicators, and main metabolic and electrolyte indicators were analyzed and compared between RCA and hfBP groups. RCA resulted in lower blood pressure than hfBP. The filter efficacy in RCA treatment was longer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiple organ dysfunction syndrome; Continuous blood purification; Blood coagulation function; Sodium citrate.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100605

Low expression of APAF-1XL in acute myeloid leukemia may be associated with the failure of remission induction therapy
Provedor de dados: 56
Autores: Benites,B.D.; Fattori,A.; Hackel,C.; Lorand-Metze,I.; De Souza,C.A.; Schulz,E.; Costa,F.F.; Saad,S.T.O.
Apoptotic protease activating factor 1 (APAF-1) has a critical role in the regulation of apoptosis. In the present study, the mRNA expression analysis of different APAF-1 transcripts (APAF-1S, APAF-1LC, APAF-1LN, and APAF-1XL) was analyzed in bone marrow samples from 37 patients with acute myeloid leukemia (newly diagnosed, with no previous treatment). APAF-1XL and APAF-1LN transcripts (with and without an extra WD-40 repeat region, respectively) were detected in all samples, although the major form expressed was APAF-1XL in 65% of the samples (group 1), while 35% of the samples expressed primarily APAF-1LN (group 2). Only 46% of the patients presented complete remission in response to remission induction therapy (represented by less than 5% marrow blasts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: APAF-1; Acute myeloid leukemia; Apoptosis; Chemotherapy resistance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700004

High plasma concentrations of asymmetric dimethylarginine inhibit ischemic cardioprotection in hypercholesterolemic rats
Provedor de dados: 56
Autores: Landim,M.B.P.; Dourado,P.M.M.; Casella-Filho,A.; Chagas,A.C.P.; da-Luz,P.L.
A low concentration of nitric oxide associated with a high concentration of asymmetric dimethylarginine (ADMA) can explain the lack of ischemic cardioprotection observed in the presence of hypercholesterolemia. The objective of the present study was to evaluate the effect of hypercholesterolemia on ischemic pre- and postconditioning and its correlation with plasma concentrations of ADMA. Male Wistar rats (6-8 weeks old) fed a 2% cholesterol diet (n = 21) for 8 weeks were compared to controls (n = 25) and were subjected to experimental myocardial infarction and reperfusion, with ischemic pre- and postconditioning. Total cholesterol and ADMA were measured in plasma before the experimental infarct and the infarct area was quantified. Weight, total cholesterol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypercholesterolemia; Cardiotonics; Nitric oxide; Risk factors; Asymmetric dimethylarginine.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500454

Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury
Provedor de dados: 56
Autores: Nicola,F.C.; Rodrigues,L.P.; Crestani,T.; Quintiliano,K.; Sanches,E.F.; Willborn,S.; Aristimunha,D.; Boisserand,L.; Pranke,P.; Netto,C.A.
Spinal cord injury (SCI) is a disabling condition resulting in deficits of sensory and motor functions, and has no effective treatment. Considering that protocols with stem cell transplantation and treadmill training have shown promising results, the present study evaluated the effectiveness of stem cells from human exfoliated deciduous teeth (SHEDs) transplantation combined with treadmill training in rats with experimental spinal cord injury. Fifty-four Wistar rats were spinalized using NYU impactor. The rats were randomly distributed into 5 groups: Sham (laminectomy with no SCI, n=10); SCI (laminectomy followed by SCI, n=12); SHEDs (SCI treated with SHEDs, n=11); TT (SCI treated with treadmill training, n=11); SHEDs+TT (SCI treated with SHEDs and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spinal cord injury; Stem cells; Treadmill training; Functional recovery; MASCIS impactor.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900604

High-impact exercise in rats prior to and during suspension can prevent bone loss
Provedor de dados: 56
Autores: Yanagihara,G.R.; Paiva,A.G.; Gasparini,G.A.; Macedo,A.P.; Frighetto,P.D.; Volpon,J.B.; Shimano,A.C.
High-impact exercise has been considered an important method for treating bone loss in osteopenic experimental models. In this study, we investigated the effects of osteopenia caused by inactivity in femora and tibiae of rats subjected to jump training using the rat tail suspension model. Eight-week-old female Wistar rats were divided into five groups (n=10 each group): jump training for 2 weeks before suspension and training during 3 weeks of suspension; jump training for 2 weeks before suspension; jump training only during suspension; suspension without any training; and a control group. The exercise protocol consisted of 20 jumps/day, 5 days/week, with a jump height of 40 cm. The bone mineral density of the femora and tibiae was measured by double...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone; Mechanical properties; Bone mineral density; Scanning electron microscopy; Jump; Rats.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300605

Effect of salbutamol on innervated and denervated rat soleus muscle
Provedor de dados: 56
Autores: Šoic-Vranic,T.; Bobinac,D.; Bajek,S.; Jerkovic,R.; Malnar-Dragojevic,D.; Nikolic,M.
The objective of the present investigation was to perform a 14-day time-course study of treatment with salbutamol, a ß2 adrenoceptor agonist, on rat soleus muscle in order to assess fiber type selectivity in the hypertrophic response and fiber type composition. Male Wistar rats were divided into four groups: control (N = 10), treated with salbutamol (N = 30), denervated (N = 30), and treated with salbutamol after denervation (N = 30). Salbutamol was injected intraperitoneally in the rats of the 2nd and 4th groups at a concentration of 0.3 mg/kg twice a day for 2 weeks. The muscles were denervated using the crush method with pean. The animals were sacrificed 3, 6, 9, 12, and 14 days after treatment. Frozen cross-sections of soleus muscle were stained for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salbutamol; Rat skeletal muscle; Soleus muscle; Denervation; Histochemistry; Muscle fibers.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200008

Biology and clinical utilization of mesenchymal progenitor cells
Provedor de dados: 56
Autores: Minguell,J.J.; Conget,P.; Erices,A.
Within the complex cellular arrangement found in the bone marrow stroma there exists a subset of nonhematopoietic cells referred to as mesenchymal progenitor cells (MPC). These cells can be expanded ex vivo and induced, either in vitro or in vivo, to terminally differentiate into at least seven types of cells: osteocytes, chondrocytes, adipocytes, tenocytes, myotubes, astrocytes and hematopoietic-supporting stroma. This broad multipotentiality, the feasibility to obtain MPC from bone marrow, cord and peripheral blood and their transplantability support the impact that the use of MPC will have in clinical settings. However, a number of fundamental questions about the cellular and molecular biology of MPC still need to be resolved before these cells can be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal progenitor cells; Transplantation; Differentiation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800003

Western blot detection of infectious bursal disease virus infection
Provedor de dados: 56
Autores: Pereira,S.R.F.G.; Travassos,C.E.P.F.; Huguenim,A.; Guimarães,A.C.C.; Silva,A.G.; Guimarães,M.A.A.M.
In order to evaluate the use of a Western blot methodology for the diagnosis of infectious bursal disease virus (IBDV) infection, chickens were experimentally infected with IBDV strains and tested for the presence of viral antigens and antibodies by a blocking Western blot test (bWB). The viral proteins obtained from the bursa of Fabricius (BF) were transferred to a nitrocellulose membrane after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the chicken sera obtained by heart puncture were used for the detection of these proteins. In order to eliminate nonspecific reactions, we used a rabbit anti-chicken serum (blocking tool). By the use of the bWB test, two distinct viral proteins of 43-kDa (VP2) and 32-kDa (VP3) were detected....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Western blot; IBDV infection; Chicken.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500011

Plasma testosterone and 11-ketotestosterone levels of male pacu Piaractus mesopotamicus (Cypriniformes, Characidae)
Provedor de dados: 56
Autores: Gazola,R.; Borella,M.I.
The levels of testosterone (T) and 11-ketotestosterone (11-KT) of the South American pacu Piaractus mesopotamicus were determined by radioimmunoassay during two stages of the reproductive cycle, i.e., resting and maturation, and the gonadosomatic index (GSI) was calculated. The highest levels of T and 11-KT were reached during the maturation stage (T = 2400 ± 56 pg/ml; 11-KT = 2300 ± 60 pg/ml) and lower levels were maintained during the resting period. The rise in androgen levels occurred with the appearance of spermatozoa in the maturation stage, when GSI was highest
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Teleost fish; Testosterone; 11-ketotestosterone; Radioimmunoassay; Reproductive cycle.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200017

Mercury toxicity in the Amazon: contrast sensitivity and color discrimination of subjects exposed to mercury
Provedor de dados: 56
Autores: Rodrigues,A.R.; Souza,C.R.B.; Braga,A.M.; Rodrigues,P.S.S.; Silveira,A.T.; Damin,E.T.B.; Côrtes,M.I.T.; Castro,A.J.O.; Mello,G.A.; Vieira,J.L.F.; Pinheiro,M.C.N.; Ventura,D.F.; Silveira,L.C.L.
We measured visual performance in achromatic and chromatic spatial tasks of mercury-exposed subjects and compared the results with norms obtained from healthy individuals of similar age. Data were obtained for a group of 28 mercury-exposed subjects, comprising 20 Amazonian gold miners, 2 inhabitants of Amazonian riverside communities, and 6 laboratory technicians, who asked for medical care. Statistical norms were generated by testing healthy control subjects divided into three age groups. The performance of a substantial proportion of the mercury-exposed subjects was below the norms in all of these tasks. Eleven of 20 subjects (55%) performed below the norms in the achromatic contrast sensitivity task. The mercury-exposed subjects also had lower red-green...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury exposure; Mercury toxicity; Contrast sensitivity; Color vision; Visual dysfunction; Amazon riverside communities.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300018

Effect of training on the ability of dual-task coordination
Provedor de dados: 56
Autores: Rosin,F.M.; Sylwan,R.P.; Galera,C.
Within the framework of the working memory model proposed by A. Baddeley and G. Hitch, a dual-task paradigm has been suggested to evaluate the capacity to perform simultaneously two concurrent tasks. This capacity is assumed to reflect the functioning of the central executive component, which appears to be impaired in patients with dysexecutive syndrome. The present study extends the investigation of an index ("mu"), which is supposed to indicate the capacity of coordination of concurrent auditory digit span and tracking tasks, by testing the influence of training on the performance in the dual task. The presentation of the same digit sequence lists or always-different lists did not differently affect the performance. The span length affected the mu...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dual task; Working memory; Central executive; Digit span; Training.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000012

Upper esophageal sphincter pressure in patients with Chagas' disease and primary achalasia
Provedor de dados: 56
Autores: Dantas,R.O.
The most important component of the upper esophageal sphincter (UES) is the cricopharyngeal muscle. During the measurement of sphincter pressure the catheter passed through the sphincter affects the pressure value. In Chagas' disease and primary achalasia there is an esophageal myenteric plexus denervation which may affect UES pressure. We measured the UES pressure of 115 patients with Chagas' disease, 28 patients with primary achalasia and 40 healthy volunteers. We used a round manometric catheter with continuous perfusion and the rapid pull-through method, performed in triplicate during apnea. Pressures were measured in four directions, and the direction with the highest pressure (anterior/posterior) and the average of the four directions were measured....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Upper esophageal sphincter; Megaesophagus; Achalasia; Chagas' disease.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500009

Study of urinary acidification in patients with idiopathic hypocitraturia
Provedor de dados: 56
Autores: Araújo,N.C.; Rebelo,M.A.P.
Hypocitraturia (HCit) is one of the most remarkable features of renal tubular acidosis, but an acidification defect is not seen in the majority of hypocitraturic patients, whose disease is denoted idiopathic hypocitraturia. In order to assess the integrity of urinary acidification mechanisms in hypocitraturic idiopathic calcium stone formers, we studied two groups of patients, hypocitraturic (HCit, N = 21, 39.5 ± 11.5 years, 11 females and 10 males) and normocitraturic (NCit, N = 23, 40.2 ± 11.7 years, 16 females and 7 males) subjects, during a short ammonium chloride loading test lasting 8 h. During the baseline period HCit patients showed significantly higher levels of titratable acid (TA). After the administration of ammonium chloride, mean urinary pH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urinary acidification; Hypocitraturia; Titratable acidity; Ammonium; Ammonium chloride load test; Lithiasis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200007

Guanylin peptides: cyclic GMP signaling mechanisms
Provedor de dados: 56
Autores: Forte,L.R.; Freeman,R.H.; Krause,W.J.; London,R.M.
Guanylate cyclases (GC) serve in two different signaling pathways involving cytosolic and membrane enzymes. Membrane GCs are receptors for guanylin and atriopeptin peptides, two families of cGMP-regulating peptides. Three subclasses of guanylin peptides contain one intramolecular disulfide (lymphoguanylin), two disulfides (guanylin and uroguanylin) and three disulfides (E. coli stable toxin, ST). The peptides activate membrane receptor-GCs and regulate intestinal Cl- and HCO3- secretion via cGMP in target enterocytes. Uroguanylin and ST also elicit diuretic and natriuretic responses in the kidney. GC-C is an intestinal receptor-GC for guanylin and uroguanylin, but GC-C may not be involved in renal cGMP pathways. A novel receptor-GC expressed in the opossum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney; Intestine; Guanylate cyclase; Chloride secretion; Sodium excretion.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100002

Expression of TERT in precancerous gastric lesions compared to gastric cancer
Provedor de dados: 56
Autores: Duarte,M.C.; Babeto,E.; Leite,K.R.M.; Miyazaki,K.; Borim,A.A.; Rahal,P.; Silva,A.E.
The objective of this study was to determine the levels of TERT mRNA and TERT protein expression in stomach precancerous lesions such as intestinal metaplasia (IM) and gastric ulcer (GU) and compare them to gastric cancer (GC). Real-time PCR was performed to detect TERT mRNA expression levels in 35 biopsies of IM, 30 of GU, and 22 of GC and their respective normal mucosas. TERT protein was detected by immunohistochemistry in 68 samples, 34 of IM, 23 of GU, and 11 of GC. Increased TERT mRNA expression levels were observed in a significant number of cases, i.e., 46% of IM, 50% of GU, and 79% of GC. The relative mean level of TERT mRNA after normalization with the β-actin reference gene and comparison with the respective adjacent normal mucosa was slightly...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gastric ulcer; Intestinal metaplasia; Gastric cancer; TERT; Gene expression; Protein expression.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200003

Clastogenic effects of different Ureaplasma urealyticum serovars on human chromosomes
Provedor de dados: 56
The possibility that Ureaplasma urealyticum might play an important role in human infertility was first raised more than 20 years ago, but this association remains speculative. Considering the hypothesis that the pathogenicity of Ureaplasma urealyticum may depend on its serotypes, the clastogenic effects of different strains of Ureaplasma urealyticum, at concentrations of 103 CCU (color changing units)/ml, 104 CCU/ml and 105 CCU/ml, were evaluated in vitro in short-term cultures of human lymphocytes. Total or partial mitotic inhibition was produced by Ureaplasma urealyticum serotypes 2, 3 and 10 independent of the concentration (103 CCU/ml, 104 CCU/ml or 105 CCU/ml) of the microorganisms employed. In contrast, the clastogenic effects observed with...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mycoplasma; Chromosome alterations; Ureaplasma urealyticum; Clastogenic effects.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600008

Long non-coding RNA LINC00261 sensitizes human colon cancer cells to cisplatin therapy
Provedor de dados: 56
Autores: Wang,Z.K.; Yang,L.; Wu,L.L.; Mao,H.; Zhou,Y.H.; Zhang,P.F.; Dai,G.H.
Colon cancer is one of the most common digestive tumors. The present study aimed to explore the functional role, as well as the underlying mechanism of long non-coding RNA LINC00261 in colon cancer. Expression of LINC00261 was analyzed in colon cancer cell lines and human normal cell lines. Acquired resistance cell lines were then built and the acquired resistance efficiency was detected by evaluating cell viability. Thereafter, the effects of LINC00261 overexpression on cisplatin-resistant colon cancer cells were measured, as well as cell apoptosis, viability, migration, and invasion. Subsequently, we investigated the interaction of LINC00261 and β-catenin. The results showed that the LINC00261 gene was down-regulated in colon cancer cell lines and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colon cancer; LINC00261; Β-catenin; Cisplatin resistance; Mechanism.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200607

RNA and DNA aptamers as potential tools to prevent cell adhesion in disease
Provedor de dados: 56
Autores: Ulrich,H.; Alves,M.J.M.; Colli,W.
Recent research has shown that receptor-ligand interactions between surfaces of communicating cells are necessary prerequisites for cell proliferation, cell differentiation and immune defense. Cell-adhesion events have also been proposed for pathological conditions such as cancer growth, metastasis, and host-cell invasion by parasites such as Trypanosoma cruzi. RNA and DNA aptamers (aptus = Latin, fit) that have been selected from combinatorial nucleic acid libraries are capable of binding to cell-adhesion receptors leading to a halt in cellular processes induced by outside signals as a consequence of blockage of receptor-ligand interactions. We outline here a novel approach using RNA aptamers that bind to T. cruzi receptors and interrupt host-cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SELEX; Aptamers; Cell adhesion; Cell invasion; Trypanosoma cruzi.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300002

Adrenomedullin and adrenotensin regulate collagen synthesis and proliferation in pulmonary arterial smooth muscle cells
Provedor de dados: 56
Autores: Li,W.; Kong,Q.Y.; Zhao,C.F.; Zhao,F.; Li,F.H.; Xia,W.; Wang,R.; Hu,Y.M.; Hua,M.
To understand the pathophysiological mechanisms of pulmonary arterial smooth muscle cell (PASMC) proliferation and extracellular-matrix accumulation in the development of pulmonary hypertension and remodeling, this study determined the effects of different doses of adrenomedullin (ADM) and adrenotensin (ADT) on PASMC proliferation and collagen synthesis. The objective was to investigate whether extracellular signal-regulated kinase (ERK1/2) signaling was involved in ADM- and ADT-stimulated proliferation of PASMCs in 4-week-old male Wistar rats (body weight: 100-150 g, n=10). The proliferation of PASMCs was examined by 5-bromo-2-deoxyuridine incorporation. A cell growth curve was generated by the Cell Counting Kit-8 method. Expression of collagen I,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenomedullin; Adrenotensin; Pulmonary arterial smooth muscle cell; Extracellular signal-regulated kinase-1; Collagen.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201047

Diastolic function is associated with quality of life and exercise capacity in stable heart failure patients with reduced ejection fraction
Provedor de dados: 56
Autores: Bussoni,M.F.; Guirado,G.N.; Roscani,M.G.; Polegato,B.F.; Matsubara,L.S.; Bazan,S.G.Z.; Matsubara,B.B.
Exercise capacity and quality of life (QOL) are important outcome predictors in patients with systolic heart failure (HF), independent of left ventricular (LV) ejection fraction (LVEF). LV diastolic function has been shown to be a better predictor of aerobic exercise capacity in patients with systolic dysfunction and a New York Heart Association (NYHA) classification ≥II. We hypothesized that the currently used index of diastolic function E/e' is associated with exercise capacity and QOL, even in optimally treated HF patients with reduced LVEF. This prospective study included 44 consecutive patients aged 55±11 years (27 men and 17 women), with LVEF&lt;0.50 and NYHA functional class I-III, receiving optimal pharmacological treatment and in a stable clinical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Echocardiography; Left ventricular dysfunction; Six-minute walk test; Dyspnea.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900803

Mutations in SRY and WT1 genes required for gonadal development are not responsible for XY partial gonadal dysgenesis
Provedor de dados: 56
Autores: Tagliarini,E.B.; Assumpção,J.G.; Scolfaro,M.R.; Mello,M.P. de; Maciel-Guerra,A.T.; Guerra Júnior,G.; Hackel,C.
The WT1 transcription factor regulates SRY expression during the initial steps of the sex determination process in humans, activating a gene cascade leading to testis differentiation. In addition to causing Wilms' tumor, mutations in WT1 are often responsible for urogenital defects in men, while SRY mutations are mainly related to 46,XY pure gonadal dysgenesis. In order to evaluate their role in abnormal testicular organogenesis, we screened for SRY and WT1 gene mutations in 10 children with XY partial gonadal dysgenesis, 2 of whom with a history of Wilms' tumor. The open reading frame and 360 bp of the 5' flanking sequence of the SRY gene, and the ten exons and intron boundaries of the WT1 gene were amplified by PCR of genomic DNA. Single-strand...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: XY partial gonadal dysgenesis; SRY open reading frame; SRY 5' untranslated region; WT1 exons; Denys-Drash syndrome.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100004

Inhibition of return, gap effect and saccadic reaction time to a visual target
Provedor de dados: 56
Autores: Guimarães-Silva,S.; Gawryszewski,L.G.; Portugal,T.S.; Klausner-de-Oliveira,L.
Simple manual reaction time (MRT) to a visual target (S2) is shortened when a non-informative cue (S1) is flashed at the S2 location shortly before the onset of S2 (early facilitation). Afterwards, MRT to S2 appearing at the S1 location is lengthened (inhibition of return - IOR). Similar results have been obtained for saccadic reaction time (SRT). Moreover, when there is a temporal gap between offset of the fixation point (FP) and onset of a target (gap paradigm), SRT is shorter than SRT in an overlap paradigm (FP remains on). In the present study, we determined SRT to S2 (10º) after presenting S1 at the same eccentricity (10º) or at a parafoveal position (2º) in the same or in the opposite hemifield. In addition, we employed both gap and overlap...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Visual attention; Saccadic reaction time; Inhibition of return; Covert orienting; Gap effect.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400010

Enhanced mucosal re-epithelialization induced by short chain fatty acids in experimental colitis
Provedor de dados: 56
Autores: Aguilar-Nascimento,J.E.; França-da-Silva,L.R.; de-Oliveira,A.F.; Gomes-da-Silva,M.H.
The short chain fatty acids (SCFA) are the best nutrients for the colonocytes. Glucose is poorly used as a fuel but may be transformed into SCFA by colonic bacteria. The aim of this study was to investigate the effect of SCFA or glucose on experimental colitis. Colitis was induced in 30 Wistar rats by colonic instillation of 4% acetic acid. Five days later they were randomized to receive twice a day colonic lavage containing saline (controls, N = 10), 10% hypertonic glucose (N = 10) or SCFA (N = 10) until day 8 when they were killed. At autopsy, the colon was removed and weighed and the mucosa was evaluated macro- and microscopically and stripped out for DNA assay. Data are reported as mean ± SD or median [range] as appropriate. All animals lost weight but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Short chain fatty acids; Ulcerative colitis; Glucose; Colon; Inflammation; Mucosa.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800005

Standardization of an isolated pig heart preparation with parabiotic circulation: methodological considerations
Provedor de dados: 56
Autores: Petrucci Júnior,O.; Oliveira,P.P.M.; Carmo,M.R.; Vieira,R.W.; Braile,D.M.
In the present study we standardized an experimental model of parabiotic circulation of isolated pig heart. The isolated heart was perfused with arterial blood from a second animal as support and submitted to regional ischemia for 30 min, followed by total ischemia for 90 min and reperfusion for 90 min. Parameters for measurement of ventricular performance using different indices measured directly or indirectly from intraventricular pressure were defined as: maximum peak pressure, final diastolic pressure, pressure developed, first derivative of maximum pressure (dP/dt max), first derivative of minimum pressure (dP/dt min), systolic stress of the left ventricle (sigmas), and maximum elastance of the left ventricle. Isolated hearts subjected to regional and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolated heart; Ischemia; Parabiotic circulation; Reperfusion; Model.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500013

Cell proliferation and migration in the jejunum of suckling rats submitted to progressive fasting
Provedor de dados: 56
Autores: Gomes,J.R.; Alvares,E.P.
Cell proliferation and migration in the intestinal crypts, and cell migration in the villus are controlled by different mechanisms in adult rats. In the present study, weanling rats and fasting rats were used to quantitatively study the correlation of cell cycle parameters and epithelial cell migration in crypts and intestinal villi. Eighteen-day-old rats received a single injection of tritiated thymidine [3H]TdR (23:00 h); half of the pups were submitted to fasting 5 h earlier. Cell proliferation was determined in radioautographs of jejunal crypts, on the basis of the labeling indices (LI) taken 1, 8, 13 and 19 h after [3H]TdR. The results showed that the labeling index did not differ 1 h or 19 h after [3H]TdR between the fed (38.7% or 48%) and fasting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell proliferation; Cell migration; Crypt and villus intestine; Weanling rats; Radioautography.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200011

Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
Provedor de dados: 56
Autores: Aragão,T.C.F.R.; Souza,P.A.S.; Uchôa,A.F.; Costa,I.R.; Bloch Jr.,C.; Campos,F.A.P.
We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cactaceae; Mandacaru; Cereus jamacaru; Cactin; 2S albumins; Methionine-rich protein.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800005

Atherosclerosis and acute arterial thrombosis in rabbits: a model using balloon desendothelization without dietary intervention
Provedor de dados: 56
Acute thrombosis can be induced in rabbits by a triggering protocol using Russell's viper venom and histamine given after 8 months of a 1% cholesterol diet and balloon desendothelization. In the present study, we tested the hypothesis that aortic desendothelization performed 4 months before the triggering protocol without a high cholesterol diet is a highly effective and less expensive way of producing arterial atherosclerosis and thrombosis. Nineteen male New Zealand white rabbits on a normal diet were studied. The control group (N = 9) received no intervention during the 4-month observation period, while the other group (N = 10) was submitted to aortic balloon desendothelization using a 4F Fogarty catheter. At the end of this period, all animals were...
Palavras-chave: Endothelium; Histamine; Russell's viper venom; Coronary artery disease.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300017

Prevalence and risk factors for HBV, HCV and HDV infections among injecting drug users from Rio de Janeiro, Brazil
Provedor de dados: 56
Autores: Oliveira,M.L.A.; Bastos,F.I.; Telles,P.R.; Yoshida,C.F.T.; Schatzmayr,H.G.; Paetzold,U.; Pauli,G.; Schreier,E.
Viral hepatitis constitutes a major health issue, with high prevalence among injecting drug users (IDUs). The present study assessed the prevalence and risk determinants for hepatitis B, C and D viruses (HBV, HCV and HDV) infections among 102 IDUs from Rio de Janeiro, Brazil. Serological markers and HCV-RNA were detected by enzyme immunoassay and nested PCR, respectively. HCV genotyping was determined by restriction fragment length polymorphism analysis (RFLP). HBsAg, anti-HBc and anti-HBs were found in 7.8, 55.8 and 24.7% of IDUs, respectively. In the final logistic regression, HBV infection was independently associated with male homosexual intercourse within the last 5 years (odds ratio (OR) 3.1; 95% confidence interval (CI) 1.1-8.8). No subject...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HBV; HDV; HCV; HCV genotypes; IDU.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900009

Peripubertal orchidectomy transitorily affects age-associated thymic involution in rats
Provedor de dados: 56
Autores: Pesic,V.; Radojevic,K.; Kosec,D.; Plecas-Solarovic,B.; Perisic,M.; Pilipovic,I.; Leposavic,G.
The role of gonadal hormones in induction and, particularly, maintenance/progression of rat thymic involution, which normally starts around puberty, was reassessed by examining the effects of peripubertal orchidectomy on thymic weight and morphometric parameters at different times up to the age of 10 months. Up to 6 months post-castration both thymic weight and cellularity in orchidectomized (Cx) rats were greater than in age-matched control rats, sham Cx (Sx). The increase in thymic cellularity reflected an increase in thymocyte proliferation rate (the proportion of proliferating cells was 18.6 ± 0.7% in 2-month-old Cx (N = 5) vs 13.4 ± 0.3% (N = 5) in age-matched Sx rats) followed by reduced sensitivity to apoptotic signals (apoptotic thymocytes were 9.8...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat thymus; Aging; Orchidectomy; Stereological analysis; Thymocyte proliferation; Thymocyte apoptosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100009

Review of the Y chromosome and hypertension
Provedor de dados: 56
Autores: Ely,D.; Turner,M.; Milsted,A.
The Y chromosome from spontaneously hypertensive rats (SHR) has a locus that raises blood pressure 20-25 mmHg. Associated with the SHR Y chromosome effect is a 4-week earlier pubertal rise of testosterone and dependence upon the androgen receptor for the full blood pressure effect. Several indices of enhanced sympathetic nervous system (SNS) activity are also associated with the SHR Y chromosome. Blockade of SNS outflow reduced the blood pressure effect. Salt sensitivity was increased by the Y chromosome as was salt appetite which was SNS dependent. A strong correlation (r = 0.57, P&amp;lt;0.001) was demonstrable between plasma testosterone and angiotensin II. Coronary collagen increased with blood pressure and the presence of the SHR Y chromosome. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sympathetic nervous system; Testosterone; Androgen receptor; Collagen.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600009

Synergistic effect of mycophenolate mofetil and angiotensin-converting enzyme inhibitor in patients with chronic allograft nephropathy
Provedor de dados: 56
Autores: Moscoso-Solorzano,G.T.; Mastroianni-Kirsztajn,G.; Ozaki,K.S.; Franco,M.F.; Pacheco-Silva,A.; Câmara,N.O.S.
Experimental data and few clinical non-randomized studies have shown that inhibition of the renin-angiotensin system by angiotensin-converting enzyme (ACE) associated or not with the use of mycophenolate mofetil (MMF) could delay or even halt the progression of chronic allograft nephropathy (CAN). In this retrospective historical study, we investigated whether ACE inhibition (ACEI) associated or not with the use of MMF has the same effect in humans as in experimental studies and what factors are associated with a clinical response. A total of 160 transplant patients with biopsy-proven CAN were enrolled. Eighty-one of them were on ACE therapy (G1) and 80 on ACEI_free therapy (G2). Patients were further stratified for the use of MMF. G1 patients showed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin-angiotensin system; Mycophenolate mofetil; Renal allograft survival; Kidney transplantation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500008

A three-phase epidemiological study of short and long sleepers in a middle-aged Chinese population: prevalence and characteristics
Provedor de dados: 56
Autores: Hao,Y.L.; Zhang,B.; Jia,F.J.; Li,X.L.; Tang,Y.; Ren,Y.Z.; Liu,W.H.
Epidemiological studies of short and long sleepers have not been conducted previously. We collected socioeconomic, psychological, and polysomnographic characteristics of 6501 parents (3252 men and 3249 women) of 4036 primary school children in Guangzhou city. The study data were collected in three phases. The overall prevalence of short (5 h or less) and long (10 h or more) sleep duration was 0.52 and 0.64%, respectively. Long sleepers had higher Eysenck Personality Questionnaire neuroticism scores [odds ratio (OR)=1.224, 95% confidence interval (CI)=1.047-1.409] and lower education levels (OR=0.740, 95%CI=0.631-0.849) than short sleepers. In the polysomnographic assessment, short, long, and normal sleepers (7-8 h) shared similar durations of Stage 3 sleep...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Short sleeper; Long sleeper; Prevalence; Characteristics; Chinese.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200157

Detection of patients with functional dyspepsia using wavelet transform applied to their electrogastrogram
Provedor de dados: 56
Autores: Chacón,M.; Curilem,G.; Acuña,G.; Defilippi,C.; Madrid,A.M.; Jara,S.
The aim of the present study was to develop a classifier able to discriminate between healthy controls and dyspeptic patients by analysis of their electrogastrograms. Fifty-six electrogastrograms were analyzed, corresponding to 42 dyspeptic patients and 14 healthy controls. The original signals were subsampled, filtered and divided into the pre-, post-, and prandial stages. A time-frequency transformation based on wavelets was used to extract the signal characteristics, and a special selection procedure based on correlation was used to reduce their number. The analysis was carried out by evaluating different neural network structures to classify the wavelet coefficients into two groups (healthy subjects and dyspeptic patients). The optimization process of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional dyspepsia; Electrogastrography; Wavelet transform; Neural networks.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200014

Rat liver responsiveness to gluconeogenic substrates during insulin-induced hypoglycemia
Provedor de dados: 56
Autores: de Souza,H.M.; Borba-Murad,G.R.; Ceddia,R.B.; Curi,R.; Vardanega-Peicher,M.; Bazotte,R.B.
Hepatic responsiveness to gluconeogenic substrates during insulin-induced hypoglycemia was investigated. For this purpose, livers were perfused with a saturating concentration of 2 mM glycerol, 5 mM L-alanine or 5 mM L-glutamine as gluconeogenic substrates. All experiments were performed 1 h after an ip injection of saline (CN group) or 1 IU/kg of insulin (IN group). The IN group showed higher (P&lt;0.05) hepatic glucose production from glycerol, L-alanine and L-glutamine and higher (P&lt;0.05) production of L-lactate, pyruvate and urea from L-alanine and L-glutamine. In addition, ip injection of 100 mg/kg glycerol, L-alanine and L-glutamine promoted glucose recovery. The results indicate that the hepatic capacity to produce glucose from gluconeogenic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin-induced hypoglycemia; Hepatic glucose production; Gluconeogenesis; Alainne; Glutamine.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600012

Flavianate, an amino acid precipitant, is a competitive inhibitor of trypsin at pH 3.0
Provedor de dados: 56
Autores: Schneedorf,J.M.; Santoro,M.M.; Mares-Guia,M.
Textile dyes bind to proteins leading to selective co-precipitation of a complex involving one protein molecule and more than one dye molecule of opposite charge in acid solutions, in a process of reversible denaturation that can be utilized for protein fractionation. In order to understand what occurs before the co-precipitation, a kinetic study using bovine ß-trypsin and sodium flavianate was carried out based on reaction progress curve techniques. The experiments were carried out using &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;-CBZ-L-Lys-p-nitrophenyl ester as substrate which was added to 50 mM sodium citrate buffer, pH 3.0, containing varying concentrations of ß-trypsin and dye. The reaction was recorded spectrophotometrically at 340 nm for 30 min, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flavianic acid; Tripsin inhibition; Textile dyes.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900001

Target-directed catalytic metallodrugs
Provedor de dados: 56
Autores: Joyner,J.C.; Cowan,J.A.
Most drugs function by binding reversibly to specific biological targets, and therapeutic effects generally require saturation of these targets. One means of decreasing required drug concentrations is incorporation of reactive metal centers that elicit irreversible modification of targets. A common approach has been the design of artificial proteases/nucleases containing metal centers capable of hydrolyzing targeted proteins or nucleic acids. However, these hydrolytic catalysts typically provide relatively low rate constants for target inactivation. Recently, various catalysts were synthesized that use oxidative mechanisms to selectively cleave/inactivate therapeutic targets, including HIV RRE RNA or angiotensin converting enzyme (ACE). These oxidative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalytic metallodrug; Artificial nuclease; Ribonuclease mimic; Artificial protease; HIV; Angiotensin converting enzyme.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600465

Early life, current socioeconomic position and serum lipids in young adulthood of participants in a cohort study initiated in 1978/1979
Provedor de dados: 56
Autores: Figueiredo,F.P.; Silva,A.A.M.; Bettiol,H.; Barbieri,M.A.; Batista,R.F.L.; Lamy Filho,F.; Silva,R.A.; Aragão,V.M.F.
The association between socioeconomic position (SEP) and serum lipids has been little studied and the results have been controversial. A total of 2063 young adults born in 1978/79 were evaluated at 23-25 years of age in the fourth follow-up of a cohort study carried out in Ribeirão Preto, SP, Brazil, corresponding to 31.8% of the original sample. Total serum cholesterol (TC), triglycerides, high-density cholesterol (HDL cholesterol) and low-density cholesterol (LDL cholesterol) were analyzed according to SEP at birth and during young adulthood. SEP was classified into tertiles of family income and a cumulative score of socioeconomic disadvantage was created. TC was 11.85 mg/100 mL lower among men of lower SEP in childhood (P &lt; 0.01) but no difference...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholesterol; HDL cholesterol; LDL cholesterol; Triglycerides; Cardiovascular diseases; Socioeconomic circumstances.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900014

[RETRACTED ARTICLE] Therapeutic effect and potential mechanism of pioglitazone in rats with severe acute pancreatitis
Provedor de dados: 56
Autores: Hai,Wang; Ping,Xu; Zhi-wen,Yang; Chun,Zhang.
Caspase recruitment domain-containing protein 9 (Card9) is located upstream of the nuclear factor kappa B (NF-κB) and p38 mitogen-activated protein kinase (MAPK) inflammatory pathways. This study investigated the therapeutic effect and potential mechanism of pioglitazone in rats with severe acute pancreatitis (SAP). SAP was induced by a retrograde infusion of 5.0% sodium taurocholate into the biliopancreatic duct of Sprague Dawley rats (n=54), which were then treated with pioglitazone. Blood and pancreatic tissues were harvested at 3, 6, and 12 h after SAP induction. Pancreatic pathological damage was evaluated by hematoxylin and eosin staining. Serum amylase, serum pro-inflammatory cytokines, and pancreatic myeloperoxidase (MPO) activities were determined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caspase recruitment domain-containing protein 9; Pioglitazone; Severe acute pancreatitis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200612

Schwann cells for spinal cord repair
Provedor de dados: 56
Autores: Oudega,M.; Moon,L.D.F.; de Almeida Leme,R.J.
The complex nature of spinal cord injury appears to demand a multifactorial repair strategy. One of the components that will likely be included is an implant that will fill the area of lost nervous tissue and provide a growth substrate for injured axons. Here we will discuss the role of Schwann cells (SCs) in cell-based, surgical repair strategies of the injured adult spinal cord. We will review key studies that showed that intraspinal SC grafts limit injury-induced tissue loss and promote axonal regeneration and myelination, and that this response can be improved by adding neurotrophic factors or anti-inflammatory agents. These results will be compared with several other approaches to the repair of the spinal cord. A general concern with repair strategies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spinal cord injury; Implantation; Regeneration; Neurotrophic factors; Clinical trial; Rehabilitation; Gene profiling.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600003

Minor segmental wall motion abnormalities detected in patients with Chagas' disease have adverse prognostic implications
Provedor de dados: 56
Autores: Pazin-Filho,A.; Romano,M.M.D.; Almeida-Filho,O.C.; Furuta,M.S.; Viviani,L.F.; Schmidt,A.; Marin-Neto,J.A.; Maciel,B.C.
Recent data from our laboratory have shown that patients with the indeterminate form of Chagas' disease can have impairment of left ventricular contractility, as evaluated by the slope of the left ventricle end-systolic pressure-dimension relationship. We also showed that Chagas' disease patients with minimal baseline wall motion abnormalities detected by two-dimensional echocardiography have more intense contractility impairment when compared to patients with the indeterminate form of the disease without this abnormality. The prognostic implications of these findings have not been established. We evaluated 59 patients (37-76 years, mean = 55 years) with different clinical forms of Chagas' disease, who had normal left ventricular global systolic function...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Echocardiography; Chagas' disease; Left ventricular function; Indeterminate form of Chagas' disease.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400008

Expression of inducible nitric oxide synthase is increased in patients with heart failure due to ischemic disease
Provedor de dados: 56
Autores: Ferreiro,C.R.; Chagas,A.C.P.; Carvalho,M.H.C.; Dantas,A.P.; Scavone,C.; Souza,L.C.B.; Buffolo,E.; Luz,P.L. da.
The objective of the present study was to determine the relationship between nitric oxide synthases (NOS) and heart failure in cardiac tissue from patients with and without cardiac decompensation. Right atrial tissue was excised from patients with coronary artery disease (CAD) and left ventricular ejection fraction (LVEF) &lt;35% (N = 10), and from patients with CAD and LVEF &gt;60% (N = 10) during cardiac surgery. NOS activity was measured by the conversion of L-[H³]-arginine to L-[H³]-citrulline. Gene expression was quantified by the competitive reverse transcription-polymerase chain reaction. Both endothelial NOS (eNOS) activity and expression were significantly reduced in failing hearts compared to non-failing hearts: 0.36 ± 0.18 vs 1.51 ± 0.31 pmol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide synthase; Left ventricle ejection fraction; Heart failure.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900005

Secondary prevention of type 1 diabetes mellitus: stopping immune destruction and promoting ß-cell regeneration
Provedor de dados: 56
Autores: Couri,C.E.B.; Foss,M.C.; Voltarelli,J.C.
Type 1 diabetes mellitus results from a cell-mediated autoimmune attack against pancreatic ß-cells. Traditional treatments involve numerous daily insulin dosages/injections and rigorous glucose control. Many efforts toward the identification of ß-cell precursors have been made not only with the aim of understanding the physiology of islet regeneration, but also as an alternative way to produce ß-cells to be used in protocols of islet transplantation. In this review, we summarize the most recent studies related to precursor cells implicated in the regeneration process. These include embryonic stem cells, pancreas-derived multipotent precursors, pancreatic ductal cells, hematopoietic stem cells, mesenchymal stem cells, hepatic oval cells, and mature ß-cells....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; SS-cell regeneration; SS-cell preservation; SS-cell precursors; Immune intervention; Stem cell.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001000002

Myosin Va is developmentally regulated and expressed in the human cerebellum from birth to old age
Provedor de dados: 56
Autores: Souza,C.C.R.; Dombroski,T.C.D.; Machado,H.R.; Oliveira,R.S.; Rocha,L.B.; Rodrigues,A.R.A.; Neder,L.; Chimelli,L.; Corrêa,V.M.A.; Larson,R.E.; Martins,A.R.
Myosin Va functions as a processive, actin-based motor molecule highly enriched in the nervous system, which transports and/or tethers organelles, vesicles, and mRNA and protein translation machinery. Mutation of myosin Va leads to Griscelli disease that is associated with severe neurological deficits and a short life span. Despite playing a critical role in development, the expression of myosin Va in the central nervous system throughout the human life span has not been reported. To address this issue, the cerebellar expression of myosin Va from newborns to elderly humans was studied by immunohistochemistry using an affinity-purified anti-myosin Va antibody. Myosin Va was expressed at all ages from the 10th postnatal day to the 98th year of life, in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myosin Va; Human cerebellum; Postnatal development; Aging; Immunohistochemical expression.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200164

Presence of ductal carcinoma in situ confers an improved prognosis for patients with T1N0M0 invasive breast carcinoma
Provedor de dados: 56
Autores: Logullo,A.F.; Godoy,A.B.; Mourão-Neto,M.; Simpson,A.J.G.; Nishimoto,I.N.; Brentani,M.M.
We have retrospectively analyzed a series of 155 sequential cases of T1N0M0 ductal carcinomas of which 51 tumors had a ductal carcinoma in situ (DCIS) component for correlation between the presence of DCIS and clinicopathological variables, recurrence and patient survival. No correlations between the presence of DCIS and age, menopausal status, size, estrogen or progesterone receptors were found. High-grade infiltrative tumors tended not to present a DCIS component (P = 0.08). Patients with tumors associated with DCIS form a subgroup with few recurrences (P = 0.003) and good survival (P = 0.008). When tumors were classified by size, an association between large tumors (&gt;1.0 cm) and increased recurrence and shortened overall survival was found. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ductal carcinoma in situ; Invasive breast carcinoma; T1N0M0; Tumor size; Recurrence; Overall survival.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800008

Toxic, antimicrobial and hemagglutinating activities of the purple fluid of the sea hare Aplysia dactylomela Rang, 1828
Provedor de dados: 56
Autores: Melo,V.M.M.; Fonseca,A.M.; Vasconcelos,I.M.; Carvalho,A.F.F.U.
The antimicrobial, hemagglutinating and toxic activities of the purple fluid of the sea hare Aplysia dactylomela are described. Intact or dialyzed purple fluid inhibited the growth of species of Gram-positive and Gram-negative bacteria and the action was not bactericidal but bacteriostatic. The active factor or factors were heat labile and sensitive to extreme pH values. The fluid preferentially agglutinated rabbit erythrocytes and, to a lesser extent, human blood cells, and this activity was inhibited by the glycoprotein fetuin, a fact suggesting the presence of a lectin. The fluid was also toxic to brine shrimp nauplii (LD50 141.25 µg protein/ml) and to mice injected intraperitoneally (LD50 201.8 ± 8.6 mg protein/kg), in a dose-dependent fashion. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aplysia; Antimicrobial; Purple fluid; Toxicity; Hemagglutinating activity.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600010

Exogenous normal lymph reduces liver injury induced by lipopolysaccharides in rats
Provedor de dados: 56
Autores: Zhao,Z.G.; Zhang,L.L.; Niu,C.Y.; Zhang,J.
The liver is one of the target organs damaged by septic shock, wherein the spread of endotoxins begins. This study aimed to investigate the effects of exogenous normal lymph (ENL) on lipopolysaccharide (LPS)-induced liver injury in rats. Male Wistar rats were randomly divided into sham, LPS, and LPS+ENL groups. LPS (15 mg/kg) was administered intravenously via the left jugular vein to the LPS and LPS+ENL groups. At 15 min after the LPS injection, saline or ENL without cell components (5 mL/kg) was administered to the LPS and LPS+ENL groups, respectively, at a rate of 0.5 mL/min. Hepatocellular injury indices and hepatic histomorphology, as well as levels of P-selectin, intercellular adhesion molecule 1 (ICAM-1), myeloperoxidase (MPO), and Na+-K+-ATPase,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exogenous normal lymph; Lipopolysaccharide; Liver injury; Intercellular adhesion molecule 1; Myeloperoxidase; Na+-K+-ATPase.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200128

Effect of melatonin on the functional recovery from experimental traumatic compression of the spinal cord
Provedor de dados: 56
Autores: Schiaveto-de-Souza,A.; da-Silva,C.A.; Defino,H.L.A.; Bel,E.A.Del.
Spinal cord injury is an extremely severe condition with no available effective therapies. We examined the effect of melatonin on traumatic compression of the spinal cord. Sixty male adult Wistar rats were divided into three groups: sham-operated animals and animals with 35 and 50% spinal cord compression with a polycarbonate rod spacer. Each group was divided into two subgroups, each receiving an injection of vehicle or melatonin (2.5 mg/kg, intraperitoneal) 5 min prior to and 1, 2, 3, and 4 h after injury. Functional recovery was monitored weekly by the open-field test, the Basso, Beattie and Bresnahan locomotor scale and the inclined plane test. Histological changes of the spinal cord were examined 35 days after injury. Motor scores were progressively...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spinal cord injury; Melatonin; Functional recovery.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000400348

In vitro and in vivo studies of pirarubicin-loaded SWNT for the treatment of bladder cancer
Provedor de dados: 56
Autores: Chen,Gang; He,Yunfeng; Wu,Xiaohou; Zhang,Yao; Luo,Chunli; Jing,Peng.
Intravesical chemotherapy is an important part of the treatment for superficial bladder cancer. However, the response to it is limited and its side effects are extensive. Functional single-walled carbon nanotubes (SWNT) have shown promise for tumor-targeted accumulation and low toxicity. In the present study, we performed in vivo and in vitro investigations to determine whether SWNT-based drug delivery could induce high tumor depression in rat bladder cancer and could decrease the side effects of pirarubicin (tetrahydropyranyl-adriamycin, THP). We modified SWNT with phospholipid-branched polyethylene glycol and constructed an SWNT-THP conjugate via a cleavable ester bond. The cytotoxicity of SWNT-THP against the human bladder cancer cell line BIU-87 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Single-walled carbon nanotubes; Bladder cancer; Drug vehicle; THP; Intravesical chemotherapy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800012

Effect of tamoxifen on the coronary vascular reactivity of spontaneously hypertensive female rats
Provedor de dados: 56
Autores: Borgo,M.V.; Lopes,A.B.; Gouvêa,S.A.; Romero,W.G.; Moyses,M.R.; Bissoli,N.S.; Abreu,G.R.
Tamoxifen has been associated with a reduction in the incidence of myocardial infarction. However, the effects of tamoxifen on coronary reactivity have not been fully elucidated. The objective of this study was to determine the effects of chronic treatment with tamoxifen on coronary vascular reactivity in spontaneously hypertensive rats (SHR). Female SHR were divided into four groups (N = 7 each): sham-operated (SHAM), sham-operated and treated with tamoxifen (10 mg/kg) by gavage for 90 days (TAMOX), ovariectomized (OVX), and ovariectomized and treated with tamoxifen (OVX+TAMOX). Mean arterial pressure (MAP), heart rate (HR), coronary perfusion pressure (CPP), and coronary vascular reactivity were measured. MAP and HR were reduced (9.42 and 11.67%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tamoxifen; Estrogen; Hypertension; Menopause; Coronary.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800008

Lesions of the commissural subnucleus of the nucleus of the solitary tract increase isoproterenol-induced water intake
Provedor de dados: 56
Autores: Blanch,G.T.; Freiria-Oliveira,A.H.; Colombari,E.; Menani,J.V.; Colombari,D.S.A.
The nucleus of the solitary tract (NTS) is the primary site of the cardiovascular afferent information about arterial blood pressure and volume. The NTS projects to areas in the central nervous system involved in cardiovascular regulation and hydroelectrolyte balance, such as the anteroventral third ventricle region and the lateral parabrachial nucleus. The aim of the present study was to investigate the effects of electrolytic lesion of the commissural NTS on water and 0.3 M NaCl intake and the cardiovascular responses to subcutaneous injection of isoproterenol. Male Holtzman rats weighing 280 to 320 g were submitted to sham lesion or electrolytic lesion of the commissural NTS (N = 6-15/group). The sham-lesioned rats had the electrode placed along the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nucleus of the solitary tract; Commissural nucleus of the rat solitary tract; Water intake; Arterial pressure; Isoproterenol; Hindbrain.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800013

The first report in Brazil of severe infection caused by community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA)
Provedor de dados: 56
Autores: Rozenbaum,R.; Sampaio,M.G.; Batista,G.S.; Garibaldi,A.M.; Terra,G.M.F.; Souza,M.J.; Vieira,E.N.; Silva-Carvalho,M.C.; Teixeira,L.A.; Figueiredo,A.M.S.
Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emergent pathogen in Brazil. However, there are no data on the prevalence of CA-MRSA. We report here the first well-characterized case of severe life-threatening CA-MRSA infection in a child living in Rio de Janeiro city. The patient had many complications including hematogenous osteomyelitis and involvement of multiple sites requiring drainage of soft-tissue abscess, and pleural and pericardial empyema. The MRSA isolates recovered were genotyped using PFGE, SCCmec typing and multilocus sequence typing. Disk diffusion tests were performed following Clinical and Laboratory Standards Institute recommendations. In addition, the presence of Panton-Valentine leukocidin (PVL) was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CA-MRSA; MRSA; PVL; S. aureus; Antimicrobial resistance.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800011

Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line
Provedor de dados: 56
Autores: Ribeiro,H.F.; Alcântara,D.F.A.; Matos,L.A.; Sousa,J.M.C.; Leal,M.F.; Smith,M.A.C.; Burbano,R.R.; Bahia,M.O.
Gastric cancer is the fourth most frequent type of cancer and the second cause of cancer mortality worldwide. The genetic alterations described so far for gastric carcinomas include amplifications and mutations of the c-ERBB2, KRAS, MET, TP53, and c-MYC genes. Chromosomal instability described for gastric cancer includes gains and losses of whole chromosomes or parts of them and these events might lead to oncogene overexpression, showing the need for a better understanding of the cytogenetic aspects of this neoplasia. Very few gastric carcinoma cell lines have been isolated. The establishment and characterization of the biological properties of gastric cancer cell lines is a powerful tool to gather information about the evolution of this malignancy, and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Carcinogenesis; Chromosomal abnormalities; Gastric cancer; Oncogenes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800004

Behavioral modulation by mutilation pictures in women
Provedor de dados: 56
Autores: Pereira,M.G.; Volchan,E.; Oliveira,L.; Machado-Pinheiro,W.; Rodrigues,J.A.; Nepomuceno,F.V.P.; Pessoa,L.
Previous studies have shown that women are more emotionally expressive than men. It is unclear, however, if women are also more susceptible to the emotional modulation of behavior imposed by an affective stimulus. To investigate this issue, we devised a task in which female subjects performed six sequential trials of visual target detection following the presentation of emotional (mutilation and erotic) or neutral pictures (domestic utensils and objects) and compared the data obtained in the present study with those described in a previous study with male subjects. The experiment consisted of three blocks of 24 pictures and each block had an approximate duration of 4 min. Our sample consisted of 36 subjects (age range: 18 to 26 years) and each subject...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Emotion; Behavior; Reaction time; Sex differences; Affective stimulus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300011

Effect of dimethylsulfoxide on sphingomyelinase activity and cholesterol metabolism in Niemann-Pick type C fibroblasts
Provedor de dados: 56
Autores: Scalco,F.B.; Giugliani,R.; Tobo,P.; Coelho,J.C.
Niemann-Pick type C (NPC) fibroblasts present a large concentration of cholesterol in their cytoplasm due to a still unidentified deficiency in cholesterol metabolism. The influence of dimethylsulfoxide (DMSO) on the amount of intracellular cholesterol was measured in 8 cultures of normal fibroblasts and in 7 fibroblast cultures from NPC patients. DMSO was added to the fibroblast cultures at three different concentrations (1, 2 and 4%, v/v) and the cultures were incubated for 24 h. Sphingomyelinase activity was significantly increased in both groups of cells only when incubated with 2% DMSO (59.4 ± 9.1 and 77.0 ± 9.1 nmol h-1 mg protein-1, controls without and with 2% DMSO, respectively; 47.7 ± 5.2 and 55.8 ± 4.1 nmol h-1 mg protein-1, NPC without and with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholesterol metabolism; Sphingomyelinase; Niemann-Pick disease; Lysosomal storage disorders.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100003

Sodium taste threshold in children and its relationship to blood pressure
Provedor de dados: 56
Autores: Arguelles,J.; Diaz,J.J.; Malaga,I.; Perillan,C.; Costales,M.; Vijande,M.
Popular science has emphasized the risks of high sodium intake and many studies have confirmed that salt intake is closely related to hypertension. The present mini-review summarizes experiments about salt taste sensitivity and its relationship with blood pressure (BP) and other variables of clinical and familial relevance. Children and adolescents from control parents (N = 72) or with at least one essential hypertensive (EHT) parent (N = 51) were investigated. Maternal questionnaires on eating habits and vomiting episodes were collected. Offspring, anthropometric, BP, and salt taste sensitivity values were recorded and blood samples analyzed. Most mothers declared that they added "little salt" when cooking. Salt taste sensitivity was inversely correlated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Children; Hypertension; Cardiovascular risk factors; Salt taste sensitivity; Pregnancy; Obesity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500017

Neuropsychological dysfunction related to earlier occupational exposure to mercury vapor
Provedor de dados: 56
Autores: Zachi,E.C.; Ventura,D.F.; Faria,M.A.M.; Taub,A.
We assessed the neuropsychological test performances of 26 patients (mean age = 41.5 ± 6.1 years; mean years of education = 9.8 ± 1.8; 20 males) diagnosed with chronic occupational mercurialism who were former workers at a fluorescent lamp factory. They had been exposed to elemental mercury for an average of 10.2 ± 3.8 years and had been away from this work for 6 ± 4.7 years. Mean urinary mercury concentrations 1 year after cessation of work were 1.8 ± 0.9 µg/g creatinine. Twenty control subjects matched for age, gender, and education (18 males) were used for comparison. Neuropsychological assessment included attention, inhibitory control, verbal and visual memory, verbal fluency, manual dexterity, visual-spatial function, executive function, and semantic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury; Occupational exposure Cognitive assessment Neuropsychological dysfunction Neuropsychological tests.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300019

Control of the phosphorylation of the astrocyte marker glial fibrillary acidic protein (GFAP) in the immature rat hippocampus by glutamate and calcium ions: possible key factor in astrocytic plasticity
Provedor de dados: 56
The present review describes recent research on the regulation by glutamate and Ca2+ of the phosphorylation state of the intermediate filament protein of the astrocytic cytoskeleton, glial fibrillary acidic protein (GFAP), in immature hippocampal slices. The results of this research are discussed against a background of modern knowledge of the functional importance of astrocytes in the brain and of the structure and dynamic properties of intermediate filament proteins. Astrocytes are now recognized as partners with neurons in many aspects of brain function with important roles in neural plasticity. Site-specific phosphorylation of intermediate filament proteins, including GFAP, has been shown to regulate the dynamic equilibrium between the polymerized and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Astrocytes; Glial fibrillary acidic protein; GFAP; Glutamate receptors; Protein phosphorylation; Calcium; Intermediate filament dynamics.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300005

Acute and chronic electrical activation of baroreceptor afferents in awake and anesthetized subjects
Provedor de dados: 56
Autores: Durand,M.T.; Fazan Jr.,R.; Salgado,M.C.O.; Salgado,H.C.
Electrical stimulation of baroreceptor afferents was used in the 1960's in several species, including human beings, for the treatment of refractory hypertension. This approach bypasses the site of baroreceptor mechanosensory transduction. Chronic electrical stimulation of arterial baroreceptors, particularly of the carotid sinus nerve (Hering's nerve), was proposed as an ultimate effort to treat refractory hypertension and angina pectoris due to the limited nature of pharmacological therapy available at that time. Nevertheless, this approach was abandoned in the early 1970's due to technical limitations of implantable devices and to the development of better-tolerated antihypertensive medications. More recently, our laboratory developed the technique of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carotid sinus nerve; Aortic depressor nerve; Hemodynamics; Baroreceptor stimulation; Refractory hypertension.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100009

Brief daily postpartum separations from the litter alter dam response to psychostimulants and to stress
Provedor de dados: 56
Autores: Silveira,P.P.; Benetti,C. da Silva; Portella,A.K.; Diehl,L.A.; Molle,R. Dalle; Lucion,A.B.; Dalmaz,C.
Neonatal handling induces several behavioral and neurochemical alterations in pups, including decreased responses to stress and reduced fear in new environments. However, there are few reports in the literature concerning the behavioral effects of this neonatal intervention on the dams during the postpartum period. Therefore, the aim of the current study was to determine if brief postpartum separation from pups has a persistent impact on the dam's stress response and behavior. Litters were divided into two neonatal groups: 1) non-handled and 2) handled [10 min/day, from postnatal day (PND) 1 to 10]. Weaning occurred at PND 21 when behavioral tasks started to be applied to the dams, including sweet food ingestion (PND 21), forced swimming test (PND 28), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feeding behavior; Neonatal handling; Psychostimulants; Stress response; Dam behavior.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500426

Circulating myeloid-derived suppressor cells predict disease activity and treatment response in patients with immune thrombocytopenia
Provedor de dados: 56
Autores: Zhou,J.; Zhou,Y.; Wen,J.; Sun,X.; Zhang,X.
Immune thrombocytopenia (ITP) is a disease characterized by isolated thrombocytopenia. Abnormal effector T cell activation is an important mechanism in the pathogenesis of ITP. Regulatory T cells (Treg) have a strong immunosuppressive function for T cell activation and their importance in the pathophysiology and clinical treatment of ITP has been confirmed. Myeloid-derived suppressor cells (MDSCs) are other immunosuppressive cells, which can also suppress T cell activation by secreting arginase, iNOS and ROS, and are essential for Treg cells’ differentiation and maturation. Therefore, we speculate that MDSCs might also be involved in the immune-dysregulation mechanism of ITP. In this study, we tested MDSCs and Treg cells in peripheral blood samples of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myeloid-derived suppressor cells; Immune thrombocytopenia; Regulatory T cells; Disease activity; Treatment response.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200704

IFN-g in human Chagas' disease: protection or pathology?
Provedor de dados: 56
Autores: Bahia-Oliveira,L.M.G.; Gomes,J.A.S.; Rocha,M.O.C.; Moreira,M.C.V.; Lemos,E.M.; Luz,Z.M.P.; Pereira,M.E.S.; Coffman,R.L.; Dias,J.C.P.; Cançado,J.R.; Gazzinelli,G.; Corrêa-Oliveira,R.
An apparently paradoxical role for IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; in human Chagas' disease was observed when studying the pattern of cytokine production by peripheral blood mononuclear cells (PBMC) obtained from two groups of chagasic patients after specific stimulation with Trypanosoma cruzi-derived antigens. The groups studied were 1) patients treated with benznidazole during the acute phase of Trypanosoma cruzi infection and 2) chronically infected untreated patients. In the treated group, higher levels of IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; were produced by PBMC from individuals cured after treatment when compared to non-cured patients. In contrast, in the chronically infected group (not treated) higher levels of IFN-&lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi Chagas' disease cardiomyopathy peripheral blood mononuclear cells (PBMC) cytokines INF-&lt; FONT FACE=Symbol&gt; G&lt; /font&gt.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100017

Variants of transcription factor 7-like 2 (TCF7L2) gene and incident glucose intolerance in Japanese-Brazilians
Provedor de dados: 56
Autores: Franco,L.F.; Crispim,F.; Pereira,A.C.; Moisés,R.S.
Common variants of the transcription factor 7-like 2 (TCF7L2) gene have been found to be associated with type 2 diabetes in different ethnic groups. The Japanese-Brazilian population has one of the highest prevalence rates of diabetes. Therefore, the aim of the present study was to assess whether two single-nucleotide polymorphisms (SNPs) of TCF7L2, rs7903146 and rs12255372, could predict the development of glucose intolerance in Japanese-Brazilians. In a population-based 7-year prospective study, we genotyped 222 individuals (72 males and 150 females, aged 56.2 ± 10.5 years) with normal glucose tolerance at baseline. In the study population, we found that the minor allele frequency was 0.05 for SNP rs7903146 and 0.03 for SNP rs12255372. No significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TCF7L2; Glucose intolerance; Japanese-Brazilians.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300011

Food restriction induces in vivo ventricular dysfunction in spontaneously hypertensive rats without impairment of in vitro myocardial contractility
Provedor de dados: 56
Autores: Okoshi,K.; Fioretto,J.R.; Okoshi,M.P.; Cicogna,A.C.; Aragon,F.F.; Matsubara,L.S.; Matsubara,B.B.
Cardiac structures, function, and myocardial contractility are affected by food restriction (FR). There are few experiments associating undernutrition with hypertension. The aim of the present study was to analyze the effects of FR on the cardiac response to hypertension in a genetic model of hypertension, the spontaneously hypertensive rat (SHR). Five-month-old SHR were fed a control or a calorie-restricted diet for 90 days. Global left ventricle (LV) systolic function was evaluated in vivo by transthoracic echocardiogram and myocardial contractility and diastolic function were assessed in vitro in an isovolumetrically beating isolated heart (Langendorff preparation). FR reduced LV systolic function (control (mean ± SD): 58.9 ± 8.2; FR: 50.8 ± 4.8%, N =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Undernutrition; Isolated heart; Echocardiogram; Langendorff preparation; Ventricular remodeling.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400019

Latent class analysis of attention and white matter correlation in children with attention-deficit/hyperactivity disorder
Provedor de dados: 56
Autores: Rossi,A.S.U.; Moura,L.M.; Miranda,M.C.; Muszkat,M.; Mello,C.B.; Bueno,O.F.A.
This study aimed to explore attentional patterns among children with inattentive attention-deficit/hyperactivity disorder (ADHD-I) and children with typical development (TD), using a latent class analysis (LCA). Patterns of brain connectivity were also explored. The sample comprised 29 ADHD-I and 29 TD matched children. An LCA was conducted to reclassify subjects according to their attentional performance, considering cognitive measures of attention and behavioral symptoms, regardless of group of origin. The new clusters were then compared in respect to brain white matter measurements (extracted from diffusion tensor imaging). Participants were rearranged in 2 new latent classes, according to their performance in an attention task and the results of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attention-deficit/hyperactivity disorder; Attention; White matter; Pattern recognition; Diffusion tensor imaging.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100604

Evaluation of 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice
Provedor de dados: 56
Autores: Carlesso,F.N.; Fuscaldi,L.L.; Araújo,R.S.; Teixeira,C.S.; Oliveira,M.C.; Fernandes,S.O.A.; Cassali,G.D.; Reis,D.C.; Barros,A.L.B.; Cardoso,V.N.
Pancreatic adenocarcinoma is important in oncology because of its high mortality rate. Deaths may be avoided if an early diagnosis could be achieved. Several types of tumors overexpress gastrin-releasing peptide receptors (GRPr), including pancreatic cancer cells. Thus, a radiolabeled peptide derivative of gastrin-releasing peptide (GRP) may be useful as a specific imaging probe. The purpose of the present study was to evaluate the feasibility of using99mTc-HYNIC-βAla-Bombesin(7-14)as an imaging probe for Capan-1 pancreatic adenocarcinoma. Xenographic pancreatic tumor was developed in nude mice and characterized by histopathological analysis. Biodistribution studies and scintigraphic images were carried out in tumor-bearing nude mice. The two methods...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 99mTc-HYNIC-βAla-Bombesin; Capan-1; Scintigraphic images; Pancreas tumor; Biodistribution.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000010

Carbohydrate antigen 125 predicts pulmonary congestion in patients with ST-segment elevation myocardial infarction
Provedor de dados: 56
Autores: Falcão,F.J.A.; Oliveira,F.R.A.; Cantarelli,F.; Cantarelli,R.; Brito-Júnior,P.; Lemos,H.; Silva,P.; Camboim,I.; Freire,M.C.; Carvalho,O.; Sobral-Filho,D.C.
Carbohydrate antigen 125 (CA125) has long been used as an ovarian cancer biomarker. However, because it is not specific for ovarian cells, CA125 could also be used to monitor congestion and inflammation in heart disease. Acute heart failure (HF) is used to identify patients with a worse prognosis in ST-segment elevation myocardial infarction (STEMI). We aimed to determine the association of CA125 with acute HF in STEMI and to compare CA125 with N-terminal pro brain natriuretic peptide (NTproBNP) with a cross-sectional study. At admission, patients were examined to define Killip class and then underwent coronary angioplasty. Blood samples, preferably taken in the hemodynamic ward, were centrifuged (1500 g for 15 min at ambient temperature) and stored at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute coronary syndrome; Pulmonary edema; CA125 antigen; Natriuretric peptide; Myocardial infarction.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200611

Inhibition of carbachol-induced inositol phosphate accumulation in the embryonic retina promoted by kainate and veratridine
Provedor de dados: 56
Autores: Sanches,G.; Ventura,A.L.M.
In the present study, we report that low concentrations of the glutamate ionotropic agonist kainate decreased the turnover of [3H]-phosphoinositides ([3H]-InsPs) induced by muscarinic receptors in the chick embryonic retina. When 100 µM carbachol was used, the estimated IC50 value for kainate was 0.2 µM and the maximal inhibition of ~50% was obtained with 1 µM or higher concentrations of the glutamatergic agonist. Our data also show that veratridine, a neurotoxin that increases the permeability of voltage-sensitive sodium channels, had no effect on [3H]-InsPs levels of the embryonic retina. However, 50 µM veratridine, but not 50 mM KCl, inhibited ~65% of the retinal response to carbachol. While carbachol increased [3H]-InsPs levels from 241.2 ± 38.0 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscarinic receptors; Phosphoinositides; Kainate; Sodium channels; Retina.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700009

Air pollution and neonatal deaths in São Paulo, Brazil
Provedor de dados: 56
Autores: Lin,C.A.; Pereira,L.A.A.; Nishioka,D.C.; Conceição,G.M.S.; Braga,A.L.F.; Saldiva,P.H.N.
Air pollution has been associated with health effects on different age groups. The present study was designed to assess the impact of daily changes in air pollutants (NO2, SO2, CO, O3, and particle matter (PM10)) on total number of daily neonatal deaths (those that occur between the first and the 28th days of life) in São Paulo, from January 1998 to December 2000, since adverse outcomes such as neonatal deaths associated with air pollution in Brazil have not been evaluated before. Generalized additive Poisson regression models were used and nonparametric smooth functions (loess) were adopted to control long-term trend, temperature, humidity, and short-term trends. A linear term was used for holidays. The association between air pollutants and neonatal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neonatal mortality; Infant mortality; Air pollution.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500019

Dopaminergic profile of new heterocyclic N-phenylpiperazine derivatives
Provedor de dados: 56
Autores: Neves,G.; Fenner,R.; Heckler,A.P.; Viana,A.F.; Tasso,L.; Menegatti,R.; Fraga,C.A.M.; Barreiro,E.J.; Dalla-Costa,T.; Rates,S.M.K.
Dopamine constitutes about 80% of the content of central catecholamines and has a crucial role in the etiology of several neuropsychiatric disorders, including Parkinson's disease, depression and schizophrenia. Several dopaminergic drugs are used to treat these pathologies, but many problems are attributed to these therapies. Within this context, the search for new more efficient dopaminergic agents with less adverse effects represents a vast research field. The aim of the present study was to report the structural design of two N-phenylpiperazine derivatives, compound 4: 1-[1-(4-chlorophenyl)-1H-4-pyrazolylmethyl]-4-phenylhexahydropyrazine and compound 5: 1-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4-ylmethyl]-4-phenylhexahydropyrazine, planned to be dopamine...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: N-phenylpiperazine derivatives; Dopamine; Apomorphine hypothermia; Amphetamine stereotypy; Catalepsy.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500010

Ginkgo biloba leaf extract (EGb 761) enhances catalepsy induced by haloperidol and L-nitroarginine in mice
Provedor de dados: 56
Autores: Fontana,L.; Souza,A.S.; Del Bel,E.A.; Oliveira,R.M.W. de.
Ginkgo biloba extract EGb 761 has been reported to have therapeutic effects which have been attributed to anti-oxidant and free radical-scavenging activities, including a direct action on nitric oxide production. L G-nitro-arginine (L-NOARG), a nitric oxide synthase inhibitor, and haloperidol, a drug that blocks dopamine receptors, are both known to induce catalepsy in rodents. Nitric oxide has been shown to influence dopaminergic transmission in the striatum. The purpose of the present study was to evaluate the effect of the extract obtained from leaves of Ginkgo biloba tree EGb 761 on catalepsy induced by haloperidol or by L-NOARG. Albino Swiss mice (35-45 g, N = 8-12) received by gavage a single or repeated oral dose (twice a day for 4 days) of EGb 761...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Ginkgo biloba; Catalepsy; Herbal medicine; Neuroleptic interaction; Extrapyramidal system.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100012

Overexpression of myeloid differentiation protein 88 in mice induces mild cardiac dysfunction, but no deficit in heart morphology
Provedor de dados: 56
Autores: Chen,W.; Huang,Z.; Jiang,X.; Li,C.; Gao,X.
Cardiac remodeling involves changes in heart shape, size, structure, and function after injury to the myocardium. The proinflammatory adaptor protein myeloid differentiation protein 88 (MyD88) contributes to cardiac remodeling. To investigate whether excessive MyD88 levels initiate spontaneous cardiac remodeling at the whole-organism level, we generated a transgenic MyD88 mouse model with a cardiac-specific promoter. MyD88 mice (male, 20-30 g, n=∼80) were born at the expected Mendelian ratio and demonstrated similar morphology of the heart and cardiomyocytes with that of wild-type controls. Although heart weight was unaffected, cardiac contractility of MyD88 hearts was mildly reduced, as shown by echocardiographic examination, compared with wild-type...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac dysfunction; Cardiac remodeling; Transgenic mice; Myeloid differentiation protein 88.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100605

The oral motor capacity and feeding performance of preterm newborns at the time of transition to oral feeding
Provedor de dados: 56
Autores: Bauer,M.A.; Prade,L.S.; Keske-Soares,M.; Haëffner,L.S.B.; Weinmann,A.R.M.
The objective of the present study was to determine the oral motor capacity and the feeding performance of preterm newborn infants when they were permitted to start oral feeding. This was an observational and prospective study conducted on 43 preterm newborns admitted to the Neonatal Intensive Care Unit of UFSM, RS, Brazil. Exclusion criteria were the presence of head and neck malformations, genetic disease, neonatal asphyxia, intracranial hemorrhage, and kernicterus. When the infants were permitted to start oral feeding, non-nutritive sucking was evaluated by a speech therapist regarding force (strong vs weak), rhythm (rapid vs slow), presence of adaptive oral reflexes (searching, sucking and swallowing) and coordination between sucking, swallowing and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Preterm; Oral motor conditions; Feeding performance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000012

A spheroid-based 3-D culture model for pancreatic cancer drug testing, using the acid phosphatase assay
Provedor de dados: 56
Autores: Wen,Z.; Liao,Q.; Hu,Y.; You,L.; Zhou,L.; Zhao,Y.
Current therapy for pancreatic cancer is multimodal, involving surgery and chemotherapy. However, development of pancreatic cancer therapies requires a thorough evaluation of drug efficacy in vitro before animal testing and subsequent clinical trials. Compared to two-dimensional culture of cell monolayer, three-dimensional (3-D) models more closely mimic native tissues, since the tumor microenvironment established in 3-D models often plays a significant role in cancer progression and cellular responses to the drugs. Accumulating evidence has highlighted the benefits of 3-D in vitro models of various cancers. In the present study, we have developed a spheroid-based, 3-D culture of pancreatic cancer cell lines MIAPaCa-2 and PANC-1 for pancreatic drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spheroid; Three-dimensional culture; Pancreatic cancer; Drug testing.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700634

Reinforcement omission effects in rats with bilateral lesions in the substantia nigra pars compacta and ventral tegmental area
Provedor de dados: 56
Autores: Tavares,T.F.; Judice-Daher,D.M.; Bueno,J.L.O.
Reinforcement omission effects (ROEs) are characterized by higher response rates after reinforcement omission than after reinforcement delivery. This pattern of behavior is interpreted in terms of motivational and attentional processes. Recent studies from our laboratory have shown that the amygdala, nucleus accumbens, and medial prefrontal cortex are involved in ROE modulation. Also, the literature has demonstrated a role of other areas such as substantia nigra pars compacta (SNc) and the ventral tegmental area (VTA) in processes related to surprising events, such as prediction error and presentation or omission of an event (exteroceptive stimulus and reinforcement). Since these structures send projections to areas related to ROE modulation such as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reinforcement omission effects; Substantia nigra pars compacta; Ventral tegmental area; Fixed interval with a limited hold; Operant behavior.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700606

Congenital ventricular septal defects and prenatal exposure to cyclooxygenase inhibitors
Provedor de dados: 56
Autores: Burdan,F.; Szumilo,J.; Dudka,J.; Korobowicz,A.; Klepacz,R.
Ventricular septal defects (VSDs) are common congenital abnormalities which have been reported to be associated with maternal fever and various environmental factors. The aim of the present study was to evaluate the effect of prenatal exposure to cyclooxygenase (COX) inhibitors on heart defects. A retrospective statistical analysis was performed using data collected in our laboratory during various teratological studies carried out on albino CRL:(WI)WUBR Wistar strain rats from 1997 to 2004. The observations were compared with concurrent and historic control data, as well as findings from other developmental toxicological studies with selective and nonselective COX-2 inhibitors. Despite the lack of significant differences in the frequency of VSDs between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclooxygenase; Cyclooxygenase inhibitor; Nonsteroidal anti-inflammatory drug; Ventricular septal defect.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700011

Lipid peroxidation, antioxidant enzymes and glutathione levels in human erythrocytes exposed to colloidal iron hydroxide in vitro
Provedor de dados: 56
Autores: Ferreira,A.L.A.; Machado,P.E.A.; Matsubara,L.S.
The free form of the iron ion is one of the strongest oxidizing agents in the cellular environment. The effect of iron at different concentrations (0, 1, 5, 10, 50, and 100 µM Fe3+) on the normal human red blood cell (RBC) antioxidant system was evaluated in vitro by measuring total (GSH) and oxidized (GSSG) glutathione levels, and superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and reductase (GSH-Rd) activities. Membrane lipid peroxidation was assessed by measuring thiobarbituric acid reactive substance (TBARS). The RBC were incubated with colloidal iron hydroxide and phosphate-buffered saline, pH 7.45, at 37oC, for 60 min. For each assay, the results for the control group were: a) GSH = 3.52 ± 0.27 µM/g Hb; b) GSSG = 0.17 ± 0.03...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalase; Superoxide dismutase; Glutathione; Iron; Erythrocyte; Thiobarbituric acid reactive substance.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600004

Cardiovascular, respiratory and metabolic responses to temperature and hypoxia of the winter frog Rana catesbeiana
Provedor de dados: 56
The objective of the present study was to determine the effects of hypoxia and temperature on the cardiovascular and respiratory systems and plasma glucose levels of the winter bullfrog Rana catesbeiana. Body temperature was maintained at 10, 15, 25 and 35oC for measurements of breathing frequency, heart rate, arterial blood pressure, metabolic rate, plasma glucose levels, blood gases and acid-base status. Reducing body temperature from 35 to 10oC decreased (P&amp;lt;0.001) heart rate (bpm) from 64.0 ± 3.1 (N = 5) to 12.5 ± 2.5 (N = 6) and blood pressure (mmHg) (P&amp;lt;0.05) from 41.9 ± 2.1 (N = 5) to 33.1 ± 2.1 (N = 6), whereas no significant changes were observed under hypoxia. Hypoxia-induced changes in breathing frequency and acid-base status were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Temperature; Hypoxia; Rana; Breathing frequency; Blood pressure; Heart rate; Acid-base status; Hyperglycemia.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100019

Elevated levels of erythrocyte-conjugated dienes indicate increased lipid peroxidation in schistosomiasis mansoni patients
Provedor de dados: 56
Autores: Facundo,H.T.F.; Brandt,C.T.; Owen,J.S.; Lima,V.L.M.
Schistosoma mansoni causes liver disease by inducing granulomatous inflammation. This favors formation of reactive oxygen species, including superoxide ions, hydrogen peroxide and hydroxyl radicals all of which may induce lipid peroxidation. We have evaluated lipid peroxidation in 18 patients with hepatosplenic schistosomiasis mansoni previously treated with oxamniquine followed by splenectomy, ligature of the left gastric vein and auto-implantation of spleen tissue, by measuring levels of erythrocyte-conjugated dienes and plasma malondialdehyde (MDA). Age-matched, healthy individuals (N = 18) formed the control group. Erythrocyte-conjugated dienes were extracted with dichloromethane/methanol and quantified by UV spectrophotometry, while plasma MDA was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lipid peroxidation; Conjugated dienes; Malondialdehyde; Schistosomiasis mansoni.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700003

Effect of a leucine-supplemented diet on body composition changes in pregnant rats bearing Walker 256 tumor
Provedor de dados: 56
Autores: Ventrucci,G.; Mello,M.A.R.; Gomes-Marcondes,M.C.C.
Cancer patients present high mobilization of host protein, with a decrease in lean body mass and body fat depletion occurring in parallel to neoplastic growth. Since leucine is one of the principal amino acids used by skeletal muscle for energy, we investigated the changes in body composition of pregnant tumor-bearing rats after a leucine-supplemented diet. Sixty pregnant Wistar rats divided into six groups were fed a normal protein diet (18%, N) or a leucine-supplemented diet (3% L-leucine, L). The pregnant groups were: control (CN), Walker 256 carcinoma-bearing rats (WN), control rats pair-fed with tumor-bearing rats (pfN), leucine-supplemented (CL), leucine-supplemented tumor-bearing (WL), and leucine-supplemented rats pair-fed with tumor-bearing rats...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Walker 256 tumor; Pregnancy; Body composition; Leucine; Carcass nitrogen; Diet supplementation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300006

Endogenous opiate analgesia induced by tonic immobility in guinea pigs
Provedor de dados: 56
Autores: Leite-Panissi,C.R.A.; Rodrigues,C.L.; Brentegani,M.R.; Menescal-de-Oliveira,L.
A function of the endogenous analgesic system is to prevent recuperative behaviors generated by tissue damage, thus preventing the emission of species-specific defensive behaviors. Activation of intrinsic nociception is fundamental for the maintenance of the behavioral strategy adopted. Tonic immobility (TI) is an inborn defensive behavior characterized by a temporary state of profound and reversible motor inhibition elicited by some forms of physical restraint. We studied the effect of TI behavior on nociception produced by the formalin and hot-plate tests in guinea pigs. The induction of TI produced a significant decrease in the number of flinches (18 ± 6 and 2 ± 1 in phases 1 and 2) and lickings (6 ± 2 and 1 ± 1 in phases 1 and 2) in the formalin test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tonic immobility; Guinea pig; Antinociception; Opioids; Naloxone; Analgesia.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200013

Mirtazapine versus fluoxetine in the treatment of panic disorder
Provedor de dados: 56
Autores: Ribeiro,L.; Busnello,J.V.; Kauer-Sant'Anna,M.; Madruga,M.; Quevedo,J.; Busnello,E.A.D.; Kapczinski,F.
Mirtazapine is an antidepressant whose side effect profile differs from that of first-line agents (selective serotonin reuptake inhibitors) used in the treatment of panic disorder. The present study compared the effect of mirtazapine and fluoxetine in the treatment of panic disorder in a double-blind, randomized, flexible-dose trial conducted with outpatients. After a 1-week single-blind placebo run-in, 27 patients entered an 8-week double-blind phase in which they were randomly assigned to treatment with either mirtazapine or fluoxetine. Both groups improved significantly in all but one efficacy measure (P&lt;=0.01). ANOVA showed no significant differences between the two treatment groups in number of panic attacks, Hamilton Anxiety Scale or Sheehan...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mirtazapine; Fluoxetine; Panic disorder; Treatment; Randomized trial.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000010

Effect of policosanol on cerebral ischemia in Mongolian gerbils
Provedor de dados: 56
Autores: Molina,V.; Arruzazabala,M.L.; Carbajal,D.; Valdés,S.; Noa,M.; Más,R.; Fraga,V.; Menéndez,R.
Policosanol is a mixture of higher aliphatic primary alcohols isolated from sugar cane wax, whose main component is octacosanol. An inhibitory effect of policosanol on platelet aggregation and cerebral ischemia in animal models has been reported. Thus, the objective of the present study was to evaluate the effect of policosanol on cerebral ischemia induced by unilateral carotid ligation and bilateral clamping and recirculation in Mongolian gerbils. Policosanol (200 mg/kg) administered immediately after unilateral carotid ligation and at 12- or 24-h intervals for 48 h significantly inhibited mortality and clinical symptoms when compared with controls, whereas lower doses (100 mg/kg) were not effective. Control animals showed swelling (tissue vacuolization)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebral ischemia; Gerbils; Policosanol; Reperfusion brain edema.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000014

Manual and semiautomatic segmentation of bone sarcomas on MRI have high similarity
Provedor de dados: 56
Autores: Dionísio,F.C.F.; Oliveira,L.S.; Hernandes,M.A.; Engel,E.E.; Rangayyan,R.M.; Azevedo-Marques,P.M.; Nogueira-Barbosa,M.H.
The aims of this study were to evaluate the intra- and interobserver reproducibility of manual segmentation of bone sarcomas in magnetic resonance imaging (MRI) studies and to compare manual and semiautomatic segmentation methods. This retrospective study included twelve osteosarcoma and eight Ewing sarcoma MRI studies performed prior to any therapeutic intervention. All cases were histopathologically confirmed. Three radiologists used 3D-Slicer software to perform manual segmentation of bone sarcomas in a blinded and independent manner. One radiologist segmented manually and also performed semiautomatic segmentation with the GrowCut tool. Segmentation exercises were timed for comparison. The dice similarity coefficient (DSC) and Hausdorff distance (HD)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ewing sarcoma; Osteosarcoma; Manual segmentation; Semiautomatic segmentation.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200605

Effects of intracutaneous injections of sterile water in patients with acute low back pain: a randomized, controlled, clinical trial
Provedor de dados: 56
Autores: Cui,J.Z.; Geng,Z.S.; Zhang,Y.H.; Feng,J.Y.; Zhu,P.; Zhang,X.B.
Intracutaneous sterile water injection (ISWI) is used for relief of low back pain during labor, acute attacks of urolithiasis, chronic neck and shoulder pain following whiplash injuries, and chronic myofascial pain syndrome. We conducted a randomized, double-blinded, placebo-controlled trial to evaluate the effect of ISWI for relief of acute low back pain (aLBP). A total of 68 patients (41 females and 27 males) between 18 and 55 years old experiencing aLBP with moderate to severe pain (scores ≥5 on an 11-point visual analogue scale [VAS]) were recruited and randomly assigned to receive either ISWIs (n=34) or intracutaneous isotonic saline injections (placebo treatment; n=34). The primary outcome was improvement in pain intensity using the VAS at 10, 45,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute low back pain; Intracutaneous injection; Sterile water; Isotonic saline.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300704

Prevalence of low trauma fractures in long-term kidney transplant patients with preserved renal function
Provedor de dados: 56
Autores: Braga Júnior,J.W.R.; Neves,R.M.S.; Pinheiro,M.M.; Frisoli Júnior,A.; Castro,C.H.M.; Szejnfeld,V.L.; Carvalho,A.B.
We evaluated the prevalence of low bone mineral density (BMD) and osteoporotic fractures in kidney transplantation (KT) patients and determined risk factors associated with osteoporotic fractures. The study was conducted on 191 patients (94 men and 97 women) with first KT for 3 years or more presenting stable and preserved renal function (serum creatinine levels lower than 2.5 mg/dl). KT patients were on immunosuppressive therapy and the cumulative doses of these drugs were also evaluated. BMD was determined by dual-energy X-ray absorptiometry at multiple sites (spine, femur and total body). Quantitative ultrasound of the calcaneus (broadband ultrasound attenuation, speed of sound, and stiffness index, SI) was also performed. Twenty-four percent (46) of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney transplantation; Bone density; Quantitative ultrasound; Osteoporosis; Fracture.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100016

A time series analysis of meteorological factors and hospital outpatient admissions for cardiovascular disease in the Northern district of Guizhou Province, China
Provedor de dados: 56
Autores: Jie,Y.; Houjin,H.; Mengxue,Y.; Wei,Q.; Jie,X.
Findings on the effects of weather on health, especially the effects of ambient temperature on overall morbidity, remain inconsistent. We conducted a time series study to examine the acute effects of meteorological factors (mainly air temperature) on daily hospital outpatient admissions for cardiovascular disease (CVD) in Zunyi City, China, from January 1, 2007 to November 30, 2009. We used the generalized additive model with penalized splines to analyze hospital outpatient admissions, climatic parameters, and covariate data. Results show that, in Zunyi, air temperature was associated with hospital outpatient admission for CVD. When air temperature was less than 10°C, hospital outpatient admissions for CVD increased 1.07-fold with each increase of 1°C, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Temperature; Cardiovascular disease; Hospital outpatient admission.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800689

Elementary screening of lymph node metastatic-related genes in gastric cancer based on the co-expression network of messenger RNA, microRNA and long non-coding RNA
Provedor de dados: 56
Autores: Song,Zhonghua; Zhao,Wenhua; Cao,Danfeng; Zhang,Jinqing; Chen,Shouhua.
Gastric cancer (GC) is the fifth most common cancer and the third leading cause of cancer-related deaths worldwide. The high mortality might be attributed to delay in detection and is closely related to lymph node metastasis. Therefore, it is of great importance to explore the mechanism of lymph node metastasis and find strategies to block GC metastasis. Messenger RNA (mRNA), microRNA (miRNA) and long non-coding RNA (lncRNA) expression data and clinical data were downloaded from The Cancer Genome Atlas (TCGA) database. A total of 908 differentially expressed factors with variance &gt;0.5 including 542 genes, 42 miRNA, and 324 lncRNA were screened using significant analysis microarray algorithm, and interaction networks were constructed using these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric cancer; Lymph node metastasis; Co-expression network; Modular analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400602

Relationship of inflammatory markers and pain in patients with head and neck cancer prior to anticancer therapy
Provedor de dados: 56
Autores: Oliveira,K.G.; von Zeidler,S.V.; Lamas,A.Z.; de Podestá,J.R.V.; Sena,A.; Souza,E.D.; Lenzi,J.; Lemos,E.M.; Gouvea,S.A.; Bissoli,N.S.
Pain is a common symptom in patients with cancer, including those with head and neck cancer (HNC). While studies suggest an association between chronic inflammation and pain, levels of inflammatory cytokines, such as C-reactive protein (CRP) and tumor necrosis factor-alpha (TNF-α), have not been correlated with pain in HNC patients who are not currently undergoing anticancer treatment. The purpose of this study was to examine the relationship between these inflammatory markers and perceived pain in HNC patients prior to anticancer therapy. The study group consisted of 127 HNC patients and 9 healthy controls. Pain was assessed using the Brief Pain Inventory (BPI), and serum levels of CRP and TNF-α were determined using the particle-enhanced turbidimetric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pain; Head and neck cancer; C-reactive protein; Tumor necrosis factor-alpha; Inflammation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700600

Fertility of male adult rats submitted to forced swimming stress
Provedor de dados: 56
Autores: Mingoti,G.Z.; Pereira,R.N.; Monteiro,C.M.R.
We investigated whether stress interferes with fertility during adulthood. Male Wistar rats (weighing 220 g in the beginning of the experiment) were forced to swim for 3 min in water at 32ºC daily for 15 days. Stress was assessed by the hot-plate test after the last stressing session. To assess fertility, control and stressed males (N = 15 per group) were mated with sexually mature normal females. Males were sacrificed after copulation. Stress caused by forced swimming was demonstrated by a significant increase in the latency of the pain response in the hot-plate test (14.6 ± 1.25 s for control males vs 26.0 ± 1.53 s for stressed males, P = 0.0004). No changes were observed in body weight, testicular weight, seminal vesicle weight, ventral prostate weight...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Forced swimming stress; Male; Fertility; Rat; Spermatogonia.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500016

Gene transfer of Chlorella vulgaris n-3 fatty acid desaturase optimizes the fatty acid composition of human breast cancer cells
Provedor de dados: 56
Autores: Xue,Meilan; Ge,Yinlin; Zhang,Jinyu; Wang,Qing; Hou,Lin.
Chlorella vulgaris has the gene of n-3 fatty acid desaturase (CvFad3), which can synthesize the precursor of n-3 polyunsaturated fatty acids (PUFAs) or convert n-6 to n-3 PUFAs. The objective of the present study was to examine whether the CvFad3 gene from C. vulgaris can be functionally and efficiently expressed in human breast cancer cells and whether its expression can exert a significant effect on cell fatty acid composition. We inserted the CvFad3 gene into the plasmid pEGFP-C3 to construct the eukaryotic expression vector pEGFP-C3-n-3 and to express the n-3 Fad gene in human breast cancer cells (MCF-7 cells). Transfection of MCF-7 cells with the recombinant vector resulted in a high expression of n-3 fatty acid desaturase. Lipid analysis indicated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorella vulgaris; CvFad3 gene; Fatty acid desaturase; Recombinant expression vector; Fatty acid composition; Breast cancer cells.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200006

Morphologic and biochemical changes in male rat lung after surgical and pharmacological castration
Provedor de dados: 56
Autores: Ojeda,M.S.; Gómez,N.N.; Gil,E.; Scardapane,L.; Gimenez,M.S.
The morphology of the rat lung was studied by light microscopy in different situations: after surgical and pharmacological castration and after administration of testosterone to the castrated rat to determine if the androgen is required to maintain the normal morphology of the lung. We also determined the effect of flutamide on the phospholipid composition of both the surfactant and microsomes of the lung. Rats were separated into five groups: I - control non-castrated rats, II - castrated rats sacrificed 21 days after castration, III - castrated rats that received testosterone daily from day 2 to day 21 after castration, IV - castrated rats that received testosterone from day 15 to day 21 after castration, and V - control rats injected with flutamide for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung; Antiandrogens; Phospholipids; Flutamide; Lung morphology.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300004

A role for angiogenesis in rheumatoid arthritis
Provedor de dados: 56
Autores: Stupack,D.G.; Storgard,C.M.; Cheresh,D.A.
Rheumatoid arthritis (RA) is a chronic debilitating disease characterized by distinct autoimmune, inflammatory and fibrovascular components which lead to synovial proliferation and joint destruction. However, existing treatments specifically target only autoimmune and inflammatory components despite the fact that neovascularization of the inflamed synovium is a hallmark of rheumatoid arthritis. Angiogenesis may contribute to synovial growth, leukocyte recruitment and tissue remodeling, thus potentiating disease progression. Although no therapies currently target angiogenesis, several existing therapies have anti-angiogenic activity. Recent advances in anti-angiogenic strategies in oncology, including the identification of integrin &lt;FONT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiogenesis; Endothelium; Arthritis; Integrin.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500011

SIRT1 negatively regulates amyloid-beta-induced inflammation via the NF-κB pathway
Provedor de dados: 56
Autores: Cao,L.; Liu,C.; Wang,F.; Wang,H.
Chronic inflammation induced by amyloid-beta (Aβ) plays a key role in the development of age-related macular degeneration (AMD), and matrix metalloproteinase-9 (MMP-9), interleukin (IL)-6, and IL-8 may be associated with chronic inflammation in AMD. Sirtuin 1 (SIRT1) regulates inflammation via inhibition of nuclear factor-kappa B (NF-κB) signaling, and resveratrol has been reported to prevent Aβ-induced retinal degeneration; therefore, we investigated whether this action was mediated via activation of SIRT1 signaling. Human adult retinal pigment epithelial (RPE) cells were exposed to Aβ, and overactivation and knockdown of SIRT1 were performed to investigate whether SIRT1 is required for abrogating Aβ-induced inflammation. We found that Aβ-induced RPE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amyloid-beta; SRT1720; Age-related macular degeneration; Barrier integrity; Tight junction; Matrix metalloproteinase-9.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800659

Wrist ultrasound analysis of patients with early rheumatoid arthritis
Provedor de dados: 56
Autores: Mendonça,J.A.; Yazbek,M.A.; Laurindo,I.M.M.; Bertolo,M.B.
In the present study, we evaluated 42 wrists using the semi-quantitative scales power Doppler ultrasound (PDUS) and gray scale ultrasound (GSUS) with scores ranging from 0 to 3 and correlated the results with clinical, laboratory and radiographic data. Twenty-one patients (17 women and 4 men) with rheumatoid arthritis according to criteria of the American College of Rheumatology were enrolled in the study from September 2008 to July 2009 at Universidade Estadual de Campinas (UNICAMP). The average disease duration was 14 months. The patients were 66.6% Caucasians and 33.3% non-Caucasians, with a mean age of 42 and 41 years, respectively. A dorsal longitudinal scan was performed by ultrasound on the radiocarpal and midcarpal joints using GE LOGIQ XP-linear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Power Doppler ultrasound; Gray scale ultrasound; Rheumatoid arthritis; Wrist; Synovitis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100002

Cytosine arabinoside-metabolizing enzyme genes are underexpressed in children with MLL gene-rearranged acute lymphoblastic leukemia
Provedor de dados: 56
Autores: Mata,J.F.; Scrideli,C.A.; Queiroz,R.P.; Mori,B.O.; Emerenciano,M.; Pombo-de-Oliveira,M.S.; Tone,L.G.
Infant acute lymphoblastic leukemia (IALL) is characterized by mixed lineage leukemia (MLL) gene rearrangements, unique gene expression profiles, poor prognosis, and drug resistance. One exception is cytosine arabinoside (Ara-C) to which IALL cells seem to be more sensitive. We quantified mRNA expression of Ara-C key enzymes in leukemic lymphoblasts from 64 Brazilian ALL children, 15 of them presenting MLL gene rearrangement, and correlated it with clinical and biological features. The diagnosis was based on morphological criteria and immunophenotyping using monoclonal antibodies. MLL gene rearrangements were detected by conventional cytogenetic analysis, RT-PCR and/or fluorescence in situ hybridization. The DCK and HENT1 expression levels were determined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytosine arabinoside; Acute lymphoblastic leukemia; Infant; Mixed lineage leukemia gene rearrangement; Gene expression.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100005

Opposite lipemic response of Wistar rats and C57BL/6 mice to dietary glucose or fructose supplementation
Provedor de dados: 56
Autores: Barbosa,C.R.; Albuquerque,E.M.V.; Faria,E.C.; Oliveira,H.C.F.; Castilho,L.N.
The metabolic effects of carbohydrate supplementation in mice have not been extensively studied. In rats, glucose- and fructose-rich diets induce hypertriacylglycerolemia. In the present study, we compared the metabolic responses to two monosaccharide supplementations in two murine models. Adult male Wistar rats (N = 80) and C57BL/6 mice (N = 60), after 3 weeks on a standardized diet, were submitted to dietary supplementation by gavage with glucose (G) or fructose (F) solutions (500 g/L), 8 g/kg body weight for 21 days. Glycemia was significantly higher in rats after fructose treatment (F: 7.9 vs 9.3 mM) and in mice (G: 6.5 vs 10 and F: 6.6 vs 8.9 mM) after both carbohydrate treatments. Triacylglycerolemia increased significantly 1.5 times in rats after G...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glucose or fructose supplementation; Triacylglycerolemia; Insulin resistance; C57BL/6 mice; Wistar rats.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300007

Abnormal nocturnal blood pressure fall in normotensive adolescents with insulin-dependent diabetes is ameliorated following glycemic improvement
Provedor de dados: 56
Autores: Ferreira,S.R.G.; Cesarini,P.R.; Vivolo,M.A.; Zanella,M.T.
Lack of the physiological nocturnal fall in blood pressure (BP) has been found in diabetics and it seems to be related to the presence of diabetic complications. The present study examined the changes in the nocturnal BP pattern of 8 normotensive insulin-dependent diabetic adolescents without nephropathy following improvement in glycemic control induced by an 8-day program of adequate diet and exercise. The same number of age- and sex-matched control subjects were studied. During the first and eighth nights of the program, BP was obtained by ambulatory BP monitoring. After a 10-min rest, 3 BP and heart rate (HR) recordings were taken and the mean values were considered to represent their awake values. The monitor was programmed to cuff insufflation every...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin/dependent diabetes mellitus; Blood pressure; Glycemic control.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400008

Preventive effect of reduced glutathione on contrast-induced nephropathy in elderly patients undergoing coronary angiography or intervention: a randomized, controlled trial
Provedor de dados: 56
Autores: Jin,B.; Wu,B.W.; Zhang,J.J.; Luo,X.P.; Shi,H.M.
Coronary angiography can be a high-risk condition for the incidence of contrast-induced nephropathy (CIN) in elderly patients. Reduced glutathione, under a variety of mechanisms, may prevent CIN in this procedure. We prospectively examined whether hydration with reduced glutathione is superior to hydration alone for prevention of CIN in an elderly Han Chinese population. A total of 505 patients (271 males and 234 females) aged 75 years or older who underwent non-emergency coronary angiography or an intervention were randomly divided into two groups. The treatment group received hydration with reduced glutathione (n=262) and the control group received hydration alone (n=243). Serum creatinine and blood urea nitrogen levels were measured prior to coronary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contrast-induced nephropathy Reduced glutathione Randomized; Controlled trial.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900839

Random amplification of polymorphic DNA reveals clonal relationships among enteropathogenic Escherichia coli isolated from non-human primates and humans
Provedor de dados: 56
Autores: Carvalho,V.M.; Irino,K.; Onuma,D.; Castro,A.F. Pestana de.
Enteropathogenic Escherichia coli (EPEC) strains are important agents of infantile diarrhea all over the world, gaining even greater importance in developing countries. EPEC have also been isolated from various animal species, but most isolates belong to serotypes that differ from those recovered from humans. However, it has been demonstrated that several isolates from non-human primates belong to the serogroups and/or serotypes related to those implicated in human disease. The objective of this study was to evaluate the genetic differences between thirteen strains isolated from non-human primates and the same number of strains isolated from human infections. Human isolates belonged to the same serogroup/serotype as the monkey strains and the evaluation...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Escherichia coli; Enteropathogenic Escherichia coli; Attaching and effacing Escherichia coli; Attaching and effacing; Monkey; Random amplified polymorphic DNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200010

Effects of simultaneous exposure to stress and nicotine on nicotine-induced locomotor activation in adolescent and adult rats
Provedor de dados: 56
Autores: Zago,A.; Leão,R.M.; Carneiro-de-Oliveira,P.E.; Marin,M.T.; Cruz,F.C.; Planeta,C.S.
Preclinical studies have shown that repeated stress experiences can result in an increase in the locomotor response to the subsequent administration of drugs of abuse, a phenomenon that has been termed behavioral cross-sensitization. Behavioral sensitization reflects neuroadaptive processes associated with drug addiction and drug-induced psychosis. Although cross-sensitization between stress- and drug-induced locomotor activity has been clearly demonstrated in adult rats, few studies have evaluated this phenomenon in adolescent rats. In the present study, we determined if the simultaneous exposure to stress and nicotine was capable of inducing behavioral sensitization to nicotine in adolescent and adult rats. To this end, adolescent (postnatal day (P)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cross-sensitization; Locomotor activity; Nicotine; Stress; Adolescence.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100006

Matching salt intake to physiological need in rats using foraging protocols
Provedor de dados: 56
Autores: Rowland,N.E.
Several studies of the quantitative relationship between sodium need and sodium intake in rats are reviewed. Using acute diuretic treatment 24 h beforehand, intake matches need fairly accurately when intake is spread out in time by using a hypotonic solution of NaCl. In contrast, using a hypertonic solution, intake is typically double the need. Using the same diuretic treatment, although the natriuresis occurs within ~1 h, the appetite appears only slowly over 24 h. Increased plasma levels of aldosterone parallel the increased intake; however, treatment with metyrapone blocks the rise in aldosterone but has no effect on appetite. Satiation of sodium appetite was studied in rats using sodium loss induced by chronic diuretic treatment and daily salt...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Furosemide; Sodium depletion; Progressive ratio; Aldosterone; Satiation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500016

What ethologically based models have taught us about the neural systems underlying fear and anxiety
Provedor de dados: 56
Autores: Canteras,N.S.; Mota-Ortiz,S.R.; Motta,S.C.
Classical Pavlovian fear conditioning to painful stimuli has provided the generally accepted view of a core system centered in the central amygdala to organize fear responses. Ethologically based models using other sources of threat likely to be expected in a natural environment, such as predators or aggressive dominant conspecifics, have challenged this concept of a unitary core circuit for fear processing. We discuss here what the ethologically based models have told us about the neural systems organizing fear responses. We explored the concept that parallel paths process different classes of threats, and that these different paths influence distinct regions in the periaqueductal gray - a critical element for the organization of all kinds of fear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fear; Predator exposure; Social threat; Fear memory; Contextual fear; Hypothalamus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400006

Imbalance of γδT17/γδTreg cells in the pathogenesis of allergic asthma induced by ovalbumin
Provedor de dados: 56
Autores: Yang,Xia; Zhang,Jing-Hong; Deng,Wang-Sheng; Li,Chao-Qian.
We aimed to explore the imbalance between the T helper 17 γδT cells (γδT17) and the regulatory γδT cells (γδTreg) in asthmatic mice. Male Balb/c mice were randomly divided into the normal control group and the asthmatic model group. The asthmatic model group mice were intraperitoneally injected with the mixture of ovalbumin (OVA)/Al(OH)3 and then activated by exposure of the animals to OVA atomization. Airway hyperresponsiveness (AHR) was determined by a non-invasive lung function machine. Hematoxylin and eosin and Alcian blue-periodic acid Schiff staining were done for histopathological analysis. Interleukin (IL)-17 and IL-35 levels in bronchoalveolar lavage fluid were detected by ELISA. The percentage of IL-17+ γδT cells and Foxp3+ γδT cells in spleen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bronchial asthma; ΓδT17 cells; ΓδTreg cells; Airway inflammation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900604

The importance of accurate anatomic assessment for the volumetric analysis of the amygdala
Provedor de dados: 56
Autores: Bonilha,L.; Kobayashi,E.; Cendes,F.; Li,L.M.
There is a wide range of values reported in volumetric studies of the amygdala. The use of single plane thick magnetic resonance imaging (MRI) may prevent the correct visualization of anatomic landmarks and yield imprecise results. To assess whether there is a difference between volumetric analysis of the amygdala performed with single plane MRI 3-mm slices and with multiplanar analysis of MRI 1-mm slices, we studied healthy subjects and patients with temporal lobe epilepsy. We performed manual delineation of the amygdala on T1-weighted inversion recovery, 3-mm coronal slices and manual delineation of the amygdala on three-dimensional volumetric T1-weighted images with 1-mm slice thickness. The data were compared using a dependent t-test. There was a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amygdala; Magnetic resonance; Volumetry; Epilepsy; Temporal lobe.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300012

Biological significance of miR-126 expression in atrial fibrillation and heart failure
Provedor de dados: 56
Autores: Wei,X.J.; Han,M.; Yang,F.Y.; Wei,G.C.; Liang,Z.G.; Yao,H.; Ji,C.W.; Xie,R.S.; Gong,C.L.; Tian,Y.
We investigated the biological significance of microRNA-126 (miR-126) expression in patients with atrial fibrillation (AF) and/or heart failure (HF) to examine the possible mechanism of miR-126-dependent AF and development of HF. A total of 103 patients were divided into three groups: AF group (18 men and 17 women, mean age: 65.62±12.72 years), HF group (17 men and 15 women, mean age: 63.95±19.71 years), and HF-AF group (20 men and 16 women, mean age: 66.56±14.37 years). Quantitative real-time PCR was used to measure relative miR-126 expression as calculated by the 2−ΔΔCt method. miR-126 was frequently downregulated in the 3 patient groups compared with controls. This reduction was significantly lower in permanent and persistent AF patients than in those...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNA-126; Atrial fibrillation; Heart failure; Heart function indices; N-terminal prohormone brain natriuretic peptide (NT-proBNP).
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001100983

Cycle modulation of insulin-like growth factor-binding protein 1 in human endometrium
Provedor de dados: 56
Autores: Corleta,H.; Capp,E.; Strowitzki,T.
Endometrium is one of the fastest growing human tissues. Sex hormones, estrogen and progesterone, in interaction with several growth factors, control its growth and differentiation. Insulin-like growth factor 1 (IGF-1) interacts with cell surface receptors and also with specific soluble binding proteins. IGF-binding proteins (IGF-BP) have been shown to modulate IGF-1 action. Of six known isoforms, IGF-BP-1 has been characterized as a marker produced by endometrial stromal cells in the late secretory phase and in the decidua. In the current study, IGF-1-BP concentration and affinity in the proliferative and secretory phase of the menstrual cycle were measured. Endometrial samples were from patients of reproductive age with regular menstrual cycles and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IGF-1; Binding protein; Endometrium.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100019

Mutations in the SRY, DAX1, SF1 and WNT4 genes in Brazilian sex-reversed patients
Provedor de dados: 56
Autores: Domenice,S.; Corrêa,R.V.; Costa,E.M.F.; Nishi,M.Y.; Vilain,E.; Arnhold,I.J.P.; Mendonca,B.B.
In most mammals, male development is triggered by the transient expression of the SRY gene, which initiates a cascade of gene interactions ultimately leading to the formation of a testis from the indifferent fetal gonad. Mutation studies have identified several genes essential for early gonadal development. We report here a molecular study of the SRY, DAX1, SF1 and WNT4 genes, mainly involved in sexual determination, in Brazilian 46,XX and 46,XY sex-reversed patients. The group of 46,XX sex-reversed patients consisted of thirteen 46,XX true hermaphrodites and four 46,XX males, and was examined for the presence of the SRY gene and for the loss of function (inactivating mutations and deletions) of DAX1 and WNT4 genes. In the second group consisting of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sex reversal; SRY gene; DAX1 gene; SF1 gene; WNT4 gene.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100020

Artificial neural network models for predicting 1-year mortality in elderly patients with intertrochanteric fractures in China
Provedor de dados: 56
Autores: Shi,L.; Wang,X.C.; Wang,Y.S.
The mortality rate of older patients with intertrochanteric fractures has been increasing with the aging of populations in China. The purpose of this study was: 1) to develop an artificial neural network (ANN) using clinical information to predict the 1-year mortality of elderly patients with intertrochanteric fractures, and 2) to compare the ANN's predictive ability with that of logistic regression models. The ANN model was tested against actual outcomes of an intertrochanteric femoral fracture database in China. The ANN model was generated with eight clinical inputs and a single output. ANN's performance was compared with a logistic regression model created with the same inputs in terms of accuracy, sensitivity, specificity, and discriminability. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artificial neural network; Intertrochanteric fracture; Outcome prediction; One-year mortality.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100993

Detection of human cytomegalovirus DNA in various blood components after liver transplantation
Provedor de dados: 56
Autores: Chen,X.Y.; Hou,P.F.; Bi,J.; Ying,C.M.
The quantification of human cytomegalovirus (HCMV DNA) by real-time PCR is currently a primary option for laboratory diagnosis of HCMV infection. However, the optimal sample material remains controversial due to the use of different PCR assays. To explore the best blood component for HCMV DNA surveillance after liver transplantation, whole blood (WB), serum (SE), and plasma (PL) specimens were collected simultaneously from targeted patients and examined for HCMV DNA using one commercially available assay. The HCMV DNA-positive rate with WB (16.67%) was higher than that with either SE or PL (8.33%, both P&lt;0.01). Quantitative DNA levels in WB were of greater magnitude than those in SE (WB-SE mean log-transformed difference, 0.99; 95%CI=0.74-1.25;...
Palavras-chave: HCMV DNA; Blood components; Real-time quantitative PCR; Liver transplantation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400340

Changes in the behavioral and immunological parameters of the mollusk Biomphalaria tenagophila induced by disruption of the circadian cycle as a consequence of continuous illumination
Provedor de dados: 56
Autores: Waissel,I.; Mineo,J.R.; Natal,C.L.
In the present investigation we studied some behavioral and immunological parameters of adult gastropod mollusk, Biomphalaria tenagophila, which have been reproducing for several generations under laboratory conditions. One group of gastropods was kept on a 14-h light/10-h dark cycle, corresponding to a regular circadian cycle, and another group was exposed to continuous light for 48 h. Animals were studied along (behavioral groups) or immediately after (immunological groups) 48 h of regular circadian cycle or continuous light conditions. Stopping/floating, dragging and sliding were the behavioral aspects considered (N = 20 for regular cycle; N = 20 for continuous illumination) and number of hemocytes/µl hemolymph was the immunological parameter studied (N...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Circadian cycle; Mollusk; Neuroimmunology; Stress; Behavior.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200014

Lyophilized allografts without pre-treatment with glutaraldehyde are more suitable than cryopreserved allografts for pulmonary artery reconstruction
Provedor de dados: 56
Autores: Olmos-Zúãiga,J.R.; Jasso-Victoria,R.; Díaz-Martínez,N.E.; Gaxiola-Gaxiola,M.O.; Sotres-Vega,A.; Heras-Romero,Y.; Baltazares-Lipp,M.; Baltazares-Lipp,M.E.; Santillán-Doherty,P.; Hernández-Jiménez,C.
Various methods are available for preservation of vascular grafts for pulmonary artery (PA) replacement. Lyophilization and cryopreservation reduce antigenicity and prevent thrombosis and calcification in vascular grafts, so both methods can be used to obtain vascular bioprostheses. We evaluated the hemodynamic, gasometric, imaging, and macroscopic and microscopic findings produced by PA reconstruction with lyophilized (LyoPA) grafts and cryopreserved (CryoPA) grafts in dogs. Eighteen healthy crossbred adult dogs of both sexes weighing between 18 and 20 kg were used and divided into three groups of six: group I, PA section and reanastomosis; group II, PA resection and reconstruction with LyoPA allograft; group III, PA resection and reconstruction with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulmonary artery graft; Cryopreservation; Lyophilization; Blood vessel bioprosthesis; Prosthesis design; Vascular procedures.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200701

Metabolite and light regulation of metabolism in plants: lessons from the study of a single biochemical pathway
Provedor de dados: 56
Autores: Oliveira,I.C.; Brenner,E.; Chiu,J.; Hsieh,M.-H.; Kouranov,A.; Lam,H.-M.; Shin,M.J.; Coruzzi,G.
We are using molecular, biochemical, and genetic approaches to study the structural and regulatory genes controlling the assimilation of inorganic nitrogen into the amino acids glutamine, glutamate, aspartate and asparagine. These amino acids serve as the principal nitrogen-transport amino acids in most crop and higher plants including Arabidopsis thaliana. We have begun to investigate the regulatory mechanisms controlling nitrogen assimilation into these amino acids in plants using molecular and genetic approaches in Arabidopsis. The synthesis of the amide amino acids glutamine and asparagine is subject to tight regulation in response to environmental factors such as light and to metabolic factors such as sucrose and amino acids. For instance, light...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amino acids; Carbon; Ammonium; Nitrogen assimilation; Gene expression; Arabidopsis thaliana.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500003

Role of p53 codon 72 polymorphism in chromosomal aberrations and mitotic index in patients with chronic hepatitis B
Provedor de dados: 56
Autores: Akbaş,H.; Yalcin,K.; Isi,H.; Tekes,S.; Atay,A.E.; Akkus,Z.; Budak,T.
Polymorphisms of the p53 gene, which participates in DNA repair, can affect the functioning of the p53 protein. The Arg and Pro variants in p53 codon 72 were shown to have different regulation properties of p53-dependent DNA repair target genes that can affect various levels of cytogenetic aberrations in chronic hepatitis B patients. The present study aimed to examine the frequency of chromosomal aberrations and the mitotic index in patients with chronic hepatitis B and their possible association with p53 gene exon 4 codon 72 Arg72Pro (Ex4+119 G&gt;C; rs1042522) polymorphism. Fifty-eight patients with chronic hepatitis B and 30 healthy individuals were genotyped in terms of the p53 gene codon 72 Arg72Pro polymorphism by PCR-RFLP. A 72-h cell culture was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B; Chromosomal aberrations; Mitotic index; P53 gene codon 72 polymorphism.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100003

Overexpression of hsa-miR-125b during osteoblastic differentiation does not influence levels of Runx2, osteopontin, and ALPL gene expression
Provedor de dados: 56
Autores: Pinto,M.T.; Nicolete,L.D.F.; Rodrigues,E.S.; Palma,P.V.B.; Orellana,M.D.; Kashima,S.; Covas,D.T.
Multipotent mesenchymal stromal cells (MSCs) were first isolated from bone marrow and then from various adult tissues including placenta, cord blood, deciduous teeth, and amniotic fluid. MSCs are defined or characterized by their ability to adhere to plastic, to express specific surface antigens, and to differentiate into osteogenic, chondrogenic, adipogenic, and myogenic lineages. Although the molecular mechanisms that control MSC proliferation and differentiation are not well understood, the involvement of microRNAs has been reported. In the present study, we investigated the role of miR-125b during osteoblastic differentiation in humans. We found that miR-125b increased during osteoblastic differentiation, as well as Runx2 and ALPL genes. To study...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multipotent mesenchymal stromal cells; Osteoblastic differentiation; Hsa-miR-125b.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800676

Effect of dietary (n-3) highly unsaturated fatty acids on growth and survival of fat snook (Centropomus parallelus, Pisces: Centropomidae) larvae during first feeding
Provedor de dados: 56
Autores: Seiffert,M.E.B.; Cerqueira,V.R.; Madureira,L.A.S.
The effect of rotifers, Brachionus rotundiformis (S-type), fed three different diets: A (rotifer fed Nannochloropsis oculata), B (rotifer fed N. oculata and baker's yeast, 1:1), and C (rotifer fed N. oculata and baker's yeast, 1:1, and enriched with Selco&lt;FONT FACE="Symbol"&gt;â&lt;/FONT&gt;), was evaluated based on the survival, growth and swim bladder inflation rate of fat snook larvae. Rotifers of treatment A had higher levels (4.58 mg/g dry weight) of eicosapentaenoic acid (EPA) than B (1.81 mg/g dry weight), and similar levels (0.04 and 0.06 mg/g dry weight, respectively) of docosahexaenoic acid (DHA). Rotifers of treatment C had the highest levels of EPA (13.2 mg/g dry weight) and DHA (6.08 mg/g dry weight). Fat snook eggs were obtained by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Larvae; Centropomus parallelus; Feeding; Highly unsaturated fatty acid.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500013

HFE gene mutations and iron status of Brazilian blood donors
Provedor de dados: 56
Autores: Santos,P.C.J.L.; Cançado,R.D.; Terada,C.T.; Rostelato,S.; Gonzales,I.; Hirata,R.D.C.; Hirata,M.H.; Chiattone,C.S.; Guerra-Shinohara,E.M.
Mutations of the HFE and TFR2 genes have been associated with iron overload. HFE and TFR2 mutations were assessed in blood donors, and the relationship with iron status was evaluated. Subjects (N = 542) were recruited at the Hemocentro da Santa Casa de São Paulo, São Paulo, Brazil. Iron status was not influenced by HFE mutations in women and was independent of blood donation frequency. In contrast, men carrying the HFE 282CY genotype had lower total iron-binding capacity (TIBC) than HFE 282CC genotype carriers. Men who donated blood for the first time and were carriers of the HFE 282CY genotype had higher transferrin saturation values and lower TIBC concentrations than those with the homozygous wild genotype for the HFE C282Y mutation. Moreover, in this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HFE; TFR2; Gene mutations; Blood donors; Iron status.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100015

Gastric emptying of water in children with severe functional fecal retention
Provedor de dados: 56
Autores: Fernandes,V.P.I.; Lima,M.C.L.; Camargo,E.E.; Collares,E.F.; Bustorff-Silva,J.M.; Lomazi,E.A.
The objective of this study was to evaluate gastric emptying (GE) in pediatric patients with functional constipation. GE delay has been reported in adults with functional constipation. Gastric emptying studies were performed in 22 children with chronic constipation, fecal retention and fecal incontinence, while presenting fecal retention and after resuming regular bowel movements. Patients (18 boys, median age: 10 years; range: 7.2 to 12.7 years) were evaluated in a tertiary pediatric gastroenterology clinic. Gastric half-emptying time of water (reference range: 12 ± 3 min) was measured using a radionuclide technique immediately after first patient evaluation, when they presented fecal impaction (GE1), and when they achieved regular bowel movements (GE2),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Functional constipation; Fecal retention; Soiling; Gastric emptying; Scintigraphy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300293

Lung hyperinflation stimulates the release of inflammatory mediators in spontaneously breathing subjects
Provedor de dados: 56
Autores: Malbouisson,L.M.S.; Szeles,T.F.; Barbalho,L.; Massoco,C.O.; Carmona,M.J.C.; Carvalho,C.R.R.; Pelosi,P.; Auler Jr.,J.O.C.
Lung hyperinflation up to vital capacity is used to re-expand collapsed lung areas and to improve gas exchange during general anesthesia. However, it may induce inflammation in normal lungs. The objective of this study was to evaluate the effects of a lung hyperinflation maneuver (LHM) on plasma cytokine release in 10 healthy subjects (age: 26.1 ± 1.2 years, BMI: 23.8 ± 3.6 kg/m²). LHM was performed applying continuous positive airway pressure (CPAP) with a face mask, increased by 3-cmH2O steps up to 20 cmH2O every 5 breaths. At CPAP 20 cmH2O, an inspiratory pressure of 20 cmH2O above CPAP was applied, reaching an airway pressure of 40 cmH2O for 10 breaths. CPAP was then decreased stepwise. Blood samples were collected before and 2 and 12 h after LHM....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung hyperinflation; Ventilator-induced lung injury; Inflammation; Cytokines.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200011

Atrial natriuretic peptide and feeding activity patterns in rats
Provedor de dados: 56
This review presents historical data about atrial natriuretic peptide (ANP) from its discovery as an atrial natriuretic factor (ANF) to its role as an atrial natriuretic hormone (ANH). As a hormone, ANP can interact with the hypothalamic-pituitary-adrenal axis (HPA-A) and is related to feeding activity patterns in the rat. Food restriction proved to be an interesting model to investigate this relationship. The role of ANP must be understood within a context of peripheral and central interactions involving different peptides and pathways
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Atrial natriuretic peptide; Corticosterone; ACTH; Food restriction.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400006

Quantitative studies of the vasculature of the carotid body in the chronically hypoxic rat
Provedor de dados: 56
Autores: Clarke,J.A.; Daly,M. de B.; Marshall,J.M.; Ead,H.W.; Hennessy,E.M.
The carotid bodies of rats made chronically hypoxic by breathing 12% O2 in a normobaric chamber (inspired PO2 91 mmHg) were compared with those of controls. Serial 5-µm sections of the organs were examined using an interactive image analysis system. The total volume of the carotid bodies was increased by 64%. The total vascular volume rose by 103% and was likely due to an increase in size of the large vessels (&amp;gt;12 µm lumen diameter) because the small vessel (5-12 µm lumen diameter) volume did not increase significantly while the small vessel density tended to decrease. The extravascular volume was increased by 57%. Expressed as a percentage of the total volume of the organ, the total vascular volume did not change, but the small vessel volume was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat carotid body; Morphology; Chronic hypoxia.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300012

A spectral analysis of the myoelectric activity of the left colon in patients with schistosomiasis mansoni
Provedor de dados: 56
Autores: Ferraz,A.A.B.
The objective of the present study was to perform a spectral analysis of the electrical activity of the left colon of patients with hepatosplenic schistosomiasis. Thirty patients were studied, divided into 2 groups: group A was composed of 14 patients (9 males and 5 females) with hepatosplenic schistosomiasis and group B was composed of 16 female patients without schistosomiasis mansoni. Three pairs of electrodes were implanted in the left colon at the moment of the surgical treatment. The signals of the electric activity of the colon were captured after postoperative recovery from the ileus and fed into a computer by means of a DATAQ data collection system which identified and captured frequencies between 0.02 and 10 Hz. Data were recorded, stored and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Left colon; Electromyography; Spectral analysis; Root mean square; Intestinal motility; Schistosomiasis mansoni.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500011

Effect of Walker 256 tumor growth on intestinal absorption of leucine, methionine and glucose in newly weaned and mature rats
Provedor de dados: 56
Autores: Gomes-Marcondes,M.C.C.; Honma,H.N.; Areas,M.A.; Cury,L.
In tumor-bearing rats, most of the serum amino acids are used for synthesis and oxidation processes by the neoplastic tissue. In the present study, the effect of Walker 256 carcinoma growth on the intestinal absorption of leucine, methionine and glucose was investigated in newly weaned and mature rats. Food intake and carcass weight were decreased in newly weaned (NT) and mature (MT) rats bearing Walker 256 tumor in comparison with control animals (NC and MC). The tumor/carcass weight ratio was higher in NT than in MT rats, whereas nitrogen balance was significantly decreased in both as compared to control animals. Glucose absorption was significantly reduced in MT rats (MT = 47.3 ± 4.9 vs MC = 99.8 ± 5.3 nmol min-1 cm-1, Kruskal-Wallis test,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Walker 256 tumor; Intestinal absorption; Amino acids; Glucose; Aging.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000017

LncRNA SNHG12 promotes cell growth and inhibits cell apoptosis in colorectal cancer cells
Provedor de dados: 56
Autores: Wang,J.Z.; Xu,C.L.; Wu,H.; Shen,S.J.
Several long non-coding RNA (lncRNA) might be correlated with the prognosis of colorectal cancer (CRC) and serve as a diagnostic and prognostic biomarker. However, the exact expression pattern of small nucleolar RNA host gene 12 (SNHG12) in colorectal cancer and its clinical significance remains unclear. The level of SNHG12 was detected by qRT-PCR in CRC tissues and CRC cells. MTT assay and colony formation assay were performed to examine the cell proliferation of CRC cells transfected with pcDNA-SNHG12 or si-SNHG12. Flow cytometry technology was used to detect cell cycle and cell apoptosis of CRC cells transfected with pcDNA-SNHG12 or si-SNHG12. The protein level of cell cycle progression-related molecules, including cyclin-dependent kinases (CDK4, CDK6),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SNHG12; Cell growth; Biomarker; Cell apoptosis; Colorectal cancer.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300604

Physiological implications of the regulation of vacuolar H+-ATPase by chloride ions
Provedor de dados: 56
Autores: Carraro-Lacroix,L.R.; Lessa,L.M.A.; Fernandez,R.; Malnic,G.
Vacuolar H+-ATPase is a large multi-subunit protein that mediates ATP-driven vectorial H+ transport across the membranes. It is widely distributed and present in virtually all eukaryotic cells in intracellular membranes or in the plasma membrane of specialized cells. In subcellular organelles, ATPase is responsible for the acidification of the vesicular interior, which requires an intraorganellar acidic pH to maintain optimal enzyme activity. Control of vacuolar H+-ATPase depends on the potential difference across the membrane in which the proton ATPase is inserted. Since the transport performed by H+-ATPase is electrogenic, translocation of H+-ions across the membranes by the pump creates a lumen-positive voltage in the absence of a neutralizing current,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: H+-ATPase; Chloride channels; Cl-/H+ Antiporter; Intracellular organelles; Plasma membranes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200002

A novel missense mutation p.L76P in the GJB2 gene causing nonsyndromic recessive deafness in a Brazilian family
Provedor de dados: 56
Autores: Batissoco,A.C.; Auricchio,M.T.B.M.; Kimura,L.; Tabith-Junior,A.; Mingroni-Netto,R.C.
Mutations in the GJB2 gene, encoding connexin 26 (Cx26), are a major cause of nonsyndromic recessive hearing loss in many countries. We report here on a novel point mutation in GJB2, p.L76P (c.227C&gt;T), in compound heterozygosity with a c.35delG mutation, in two Brazilian sibs, one presenting mild and the other profound nonsyndromic neurosensorial hearing impairment. Their father, who carried a wild-type allele and a p.L76P mutation, had normal hearing. The mutation leads to the substitution of leucine (L) by proline (P) at residue 76, an evolutionarily conserved position in Cx26 as well as in other connexins. This mutation is predicted to affect the first extracellular domain (EC1) or the second transmembrane domain (TM2). EC1 is important for...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: GJB2 gene Connexin 26 Hearing impairment p.L76P c.227C&gt; T.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200004

Relationship between pulmonary function and indoor air pollution from coal combustion among adult residents in an inner-city area of southwest China
Provedor de dados: 56
Autores: Jie,Y.; Houjin,H.; Xun,M.; Kebin,L.; Xuesong,Y.; Jie,X.
Few studies evaluate the amount of particulate matter less than 2.5 mm in diameter (PM2.5) in relation to a change in lung function among adults in a population. The aim of this study was to assess the association of coal as a domestic energy source to pulmonary function in an adult population in inner-city areas of Zunyi city in China where coal use is common. In a cross-sectional study of 104 households, pulmonary function measurements were assessed and compared in 110 coal users and 121 non-coal users (≥18 years old) who were all nonsmokers. Several sociodemographic factors were assessed by questionnaire, and ventilatory function measurements including forced vital capacity (FVC), forced expiratory volume in 1 s (FEV1), the FEV1/FVC ratio, and peak...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indoor air pollution; Coal combustion; Lung function; Particulate matter.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100982

Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture
Provedor de dados: 56
Autores: Diniz,S.F.; Amorim,F.P.L.G.; Cavalcante-Neto,F.F.; Bocca,A.L.; Batista,A.C.; Simm,G.E.P.M.; Silva,T.A.
A closed fracture was performed on the left tibia of 3-month-old Wistar rats weighing 250 to 350 g that were either healthy (N = 24) or made diabetic with alloxan (N = 24) to investigate the effect of alloxan-induced diabetes on the course of bone fracture healing. Histomorphometric analysis of the fracture site was performed at 7, 14, 25, and 35 days. After 7 days, diabetic rats had significantly less cartilage (P = 0.045) and greater fibrous connective (P = 0.006) tissue formation at the fracture site compared to controls. In contrast, marked callus formation was seen in diabetic rats with significant osteogenesis (P = 0.011, P = 0.010, P = 0.010, respectively, for 14, 25, and 35 days) and chondrogenesis (P = 0.028, P = 0.033, P = 0.019) compared to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alloxan; Diabetes; Fracture repair; Closed fracture.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500005

Biomechanics and structural adaptations of the rat femur after hindlimb suspension and treadmill running
Provedor de dados: 56
Autores: Shimano,M.M.; Volpon,J.B.
We microscopically and mechanically evaluated the femurs of rats subjected to hindlimb unloading (tail suspension) followed by treadmill training. Female Wistar rats were randomly divided into five groups containing 12-14 rats: control I (118 days old), control II (139 days old), suspended (tail suspension for 28 days), suspended-released (released for 21 days after 28 days of suspension), and suspended-trained (trained for 21 days after 28 days of suspension). We measured bone resistance by bending-compression mechanical tests of the entire proximal half of the femur and three-point bending tests of diaphyseal cortical bone. We determined bone microstructure by tetracycline labeling of trabecular and cortical bone. We found that tail suspension weakened...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hindlimb suspension; Exercise; Biomechanics; Femur; Rat.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400004

Expression of MMP-2 and MMP-9 in the rat trigeminal ganglion during the development of temporomandibular joint inflammation
Provedor de dados: 56
Autores: Nascimento,G.C.; Rizzi,E.; Gerlach,R.F.; Leite-Panissi,C.R.A.
Orofacial pain is a prevalent symptom in modern society. Some musculoskeletal orofacial pain is caused by temporomandibular disorders (TMDs). This condition has a multi-factorial etiology, including emotional factors and alteration of the masticator muscle and temporomandibular joints (TMJs). TMJ inflammation is considered to be a cause of pain in patients with TMD. Extracellular proteolytic enzymes, specifically the matrix metalloproteinases (MMPs), have been shown to modulate inflammation and pain. The purpose of this investigation was to determine whether the expression and level of gelatinolytic activity of MMP-2 and MMP-9 in the trigeminal ganglion are altered during different stages of temporomandibular inflammation, as determined by gelatin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Persistent temporomandibular inflammation; Matrix metalloproteinases; Trigeminal ganglion.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100956

High prevalence of methicillin resistance and PVL genes among Staphylococcus aureus isolates from the nares and skin lesions of pediatric patients with atopic dermatitis
Provedor de dados: 56
Autores: Cavalcante,F.S.; Abad,E.D.; Lyra,Y.C.; Saintive,S.B.; Ribeiro,M.; Ferreira,D.C.; Santos,K.R.N. dos.
Staphylococcus aureus is highly prevalent among patients with atopic dermatitis (AD), and this pathogen may trigger and aggravate AD lesions. The aim of this study was to determine the prevalence of S. aureus in the nares of pediatric subjects and verify the phenotypic and molecular characteristics of the isolates in pediatric patients with AD. Isolates were tested for antimicrobial susceptibility, SCCmec typing, and Panton-Valentine Leukocidin (PVL) genes. Lineages were determined by pulsed-field gel electrophoresis and multilocus sequence typing (MLST). AD severity was assessed with the Scoring Atopic Dermatitis (SCORAD) index. Among 106 patients, 90 (85%) presented S. aureus isolates in their nares, and 8 also presented the pathogen in their skin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atopic dermatitis; Staphylococcus aureus; Nasal colonization; Skin lesions; SCORAD.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700588

Adrenergic receptor β3 is involved in the memory consolidation process in mice
Provedor de dados: 56
Autores: Souza-Braga,P.; Lorena,F.B.; Nascimento,B.P.P.; Marcelino,C.P.; Ravache,T.T.; Ricci,E.; Bernardi,M.M.; Ribeiro,M.O.
Attention and emotion have a positive impact on memory formation, which is related to the activation of the noradrenergic system in the brain. The hippocampus and amygdala are fundamental structures in memory acquisition, which is modulated by noradrenaline through the noradrenergic receptors. Pharmacological studies suggest that memory acquisition depends on the action of both the β3 (β3-AR) and β2 (β2-AR) receptor subtypes. However, the use of animal models with specific knockout for the β3-AR receptor only (β3-ARKO) allows researchers to more accurately assess its role in memory formation processes. In the present study, we evaluated short- and long-term memory acquisition capacity in β3-ARKO mice and wild-type mice at approximately 60 days of age. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenergic receptors; Memory; GLUT3; Β3-AR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000607

Continuous blood purification treatment for endotoxin-induced acute respiratory distress syndrome
Provedor de dados: 56
Autores: Jiang,Y.; Lin,R.; Xu,Y.; Zhang,S.; Cui,K.; Zhu,M.; Li,A.; Chen,C.; Yang,J.; Yang,W.
This study aimed to explore the effects of continuous blood purification (CBP) treatment in pigs affected with acute respiratory distress syndrome (ARDS). A total of 12 healthy male pigs, weighing 12±1.8 kg, were randomly and equally assigned to the control and experimental groups. The ARDS pig model was prepared by intravenous injections of endotoxin (20 µg/kg). The control group was given conventional supportive therapy, while the experimental group was given continuous veno-venous hemofiltration therapy. During the treatment process, the variations in dynamic lung compliance, oxygenation index, hemodynamics, and urine volume per hour at different times (Baseline, 0, 2, 4, and 6 h) were recorded. The levels of tumor necrosis factor (TNF-α), interleukin 6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute respiratory distress syndrome; Continuous blood purification treatment; Endotoxin; Hemodynamics.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200604

Distribution of the a2, a3, and a5 nicotinic acetylcholine receptor subunits in the chick brain
Provedor de dados: 56
Autores: Torrão,A.S.; Lindstrom,J.M.; Britto,L.R.G.
Nicotinic acetylcholine receptors (nAChRs) are ionotropic receptors comprised of &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; and ß subunits. These receptors are widely distributed in the central nervous system, and previous studies have revealed specific patterns of localization for some nAChR subunits in the vertebrate brain. In the present study we used immunohistochemical methods and monoclonal antibodies to localize the &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;2, &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;3, and &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;5 nAChR subunits in the chick mesencephalon and diencephalon. We observed a differential distribution of these three subunits in the chick brain, and showed that the somata and neuropil of many central...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholinergic system; Neurotransmitters; Receptors; Receptor subunits.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000011

Blood flow measurements in rats using four color microspheres during blockade of different vasopressor systems
Provedor de dados: 56
Autores: De Angelis,K.; Gama,V.M.; Farah,V.A.M.; Irigoyen,M.C.
The use of colored microspheres to adequately evaluate blood flow changes under different circumstances in the same rat has been validated with a maximum of three different colors due to methodological limitations. The aim of the present study was to validate the use of four different colors measuring four repeated blood flow changes in the same rat to assess the role of vasopressor systems in controlling arterial pressure (AP). Red (150,000), white (200,000), yellow (150,000), and blue (200,000) colored microspheres were infused into the left ventricle of 6 male Wistar rats 1) at rest and 2) after vasopressin (aAVP, 10 µg/kg, iv), 3) renin-angiotensin (losartan, 10 mg/kg, iv), and 4) sympathetic system blockade (hexamethonium, 20 mg/kg, iv) to determine...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Colored microspheres; Arterial pressure; Sympathetic system; Renin-angiotensin; Vasopressin; Local blood flow measurement.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100018

Attention in schizophrenia and in epileptic psychosis
Provedor de dados: 56
Autores: Kairalla,I.C.J; Mattos,P.E.L; Hoexter,M.Q; Bressan,R.A; Mari,J.J; Shirakawa,I.
The adaptive behavior of human beings is usually supported by rapid monitoring of outstanding events in the environment. Some investigators have suggested that a primary attention deficit might trigger symptoms of schizophrenia. In addition, researchers have long discussed the relationship between schizophrenia and the schizophrenia-like psychosis of epilepsy (SLPE). On the basis of these considerations, the objective of the present study was to investigate attention performance of patients with both disorders. Patient age was 18 to 60 years, and all patients had received formal schooling for at least four years. Patients were excluded if they had any systemic disease with neurologic or psychiatric comorbidity, or a history of brain surgery. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attention; Schizophrenia; Psychotic disorders; Epilepsy; Neuropsychology.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100010

Protein-energy malnutrition halts hemopoietic progenitor cells in the G0/G1 cell cycle stage, thereby altering cell production rates
Provedor de dados: 56
Autores: Borelli,P.; Barros,F.E.V.; Nakajima,K.; Blatt,S.L.; Beutler,B.; Pereira,J.; Tsujita,M.; Favero,G.M.; Fock,R.A.
Protein energy malnutrition (PEM) is a syndrome that often results in immunodeficiency coupled with pancytopenia. Hemopoietic tissue requires a high nutrient supply and the proliferation, differentiation and maturation of cells occur in a constant and balanced manner, sensitive to the demands of specific cell lineages and dependent on the stem cell population. In the present study, we evaluated the effect of PEM on some aspects of hemopoiesis, analyzing the cell cycle of bone marrow cells and the percentage of progenitor cells in the bone marrow. Two-month-old male Swiss mice (N = 7-9 per group) were submitted to PEM with a low-protein diet (4%) or were fed a control diet (20% protein) ad libitum. When the experimental group had lost about 20% of their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malnutrition; Hemopoiesis; Cell cycle; Low protein diet.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600008

IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract
Provedor de dados: 56
Autores: Maciel,M.; Fusaro,A.E.; Oliveira,C.R.; Futata,E.A.; Duarte,A.J.S.; Sato,M.N.
Induced oral tolerance to mucosal-exposed antigens in immunized animals is of particular interest for the development of immunotherapeutic approaches to human allergic diseases. This is a unique feature of mucosal surfaces which represent the main contact interface with the external environment. However, the influence of oral tolerance on specific and natural polyreactive IgA antibodies, the major defense mechanism of the mucosa, is unknown. We have shown that oral administration of an extract of the dust mite Dermatophagoides pteronyssinus (Dp) to primed mice caused down-regulation of IgE responses and an increase in tumor growth factor-ß secretion. In the present study, we observed that primed inbred female A/Sn mice (8 to 10 weeks old) fed by gavage a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mucosa; Atural IgA antibodies; Oral tolerance; Cytokines; Allergy; Dermatophagoides pteronyssinus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600006

Effect of a single tetanus-diphtheria vaccine dose on the immunity of elderly people in São Paulo, Brazil
Provedor de dados: 56
Autores: Weckx,L.Y.; Divino-Goes,K.; Lihama,D.M.; Carraro,E.; Bellei,N.; Granato,C.F.H.; de Moraes-Pinto,M.I.
Epidemiological data regarding tetanus and diphtheria immunity in elderly people in Brazil are scarce. During the First National Immunization Campaign for the Elderly in Brazil in April 1999, 98 individuals (median age: 84 years) received one tetanus-dyphtheria (Td) vaccine dose (Butantan Institute, lot number 9808079/G). Inclusion criteria were elderly individuals without a history of severe immunosuppressive disease, acute infectious disease or use of immunomodulators. Blood samples were collected immediately before the vaccine and 30 days later. Serum was separated and stored at -20ºC until analysis. Tetanus and diphtheria antibodies were measured by the double-antigen ELISA test. Tetanus and diphtheria antibody concentrations lower than 0.01 IU/mL were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elderly; Tetanus; Diphtheria; Immunization.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400012

Antinociceptive effects of the essential oil of Mentha x villosa leaf and its major constituent piperitenone oxide in mice
Provedor de dados: 56
Autores: Sousa,P.J.C.; Linard,C.F.B.M.; Azevedo-Batista,D.; Oliveira,A.C.; Coelho-de-Souza,A.N.; Leal-Cardoso,J.H.
Mentha x villosa Huds (Labiatae) is an aromatic herb widely used in folk medicine. Since the essential oil of the herb has many pharmacological activities, including antispasmodic effects, we determined whether the oil and its major constituent, piperitenone oxide (PO), have antinociceptive activity. The essential oil of M. x villosa (EOMV) and PO administered orally at 200 mg/kg (vehicle: 0.1% Tween 80 in water) significantly reduced the writhings induced by acetic acid from control values of 59.5 ± 3.1 s (N = 10) to 31.9 ± 2.8 s (N = 10) and 23.8 ± 3.4 s (N = 10), respectively. When administered at 100 and 200 mg/kg, EOMV reduced the paw licking time for the second phase of the formalin test from the control value of 20.6 ± 2.1 s (N = 13) to 5.3 ± 2.2 s...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mentha x villosa essential oil; Analgesia; Nociception; Anti-inflammatory effect; Piperitenone oxide.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700010

Relationship between ventilation heterogeneity and exercise intolerance in adults with sickle cell anemia
Provedor de dados: 56
Autores: Lopes,A.J.; Marinho,C.L.; Alves,U.D.; Gonçalves,C.E.A.; Silva,P.O.; Botelho,E.C.; Bedirian,R.; Soares,A.R.; Maioli,M.C.P.
Sickle cell anemia (SCA) causes dysfunction of multiple organs, with pulmonary involvement as a major cause of mortality. Recently, there has been growing interest in the nitrogen single-breath washout (N2SBW) test, which is able to detect ventilation heterogeneity and small airway disease when the results of other pulmonary function tests (PFTs) are still normal. Thus, the objectives of the present study were to assess the heterogeneity in the ventilation distribution in adults with SCA and to determine the association between the ventilation distribution and the clinical, cardiovascular, and radiological findings. This cross-sectional study included 38 adults with SCA who underwent PFTs, echocardiography, computed tomography (CT), and 6-min walk test. To...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sickle cell anemia; Respiratory mechanics; Respiratory function tests; Ventilation; Exercise.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800607

Role of the extracellular matrix in variations of invasive pathways in lung cancers
Provedor de dados: 56
Autores: de Sá,V.K.; Carvalho,L.; Gomes,A.; Alarcão,A.; Silva,M.R.; Couceiro,P.; Sousa,V.; Soares,F.A.; Capelozzi,V.L.
Among the most common features of highly invasive tumors, such as lung adenocarcinomas (AD) and squamous cell carcinomas (SqCC), is the massive degradation of the extracellular matrix. The remarkable qualitative and quantitative modifications of hyaluronidases (HAases), hyaluronan synthases (HAS), E-cadherin adhesion molecules, and the transforming growth factor β (TGF-β) may favor invasion, cellular motility, and proliferation. We examined HAase proteins (Hyal), HAS, E-cadherin, and TGF-β profiles in lung AD subtypes and SqCC obtained from smokers and non-smokers. Fifty-six patients, median age 64 years, who underwent lobectomy for AD (N = 31) and SqCC (N = 25) were included in the study. HAS-1, -2 and -3, and Hyal-1 and -3 were significantly more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung cancer E-cadherin TGF-β HAS-1; HAS-2 and HAS-3 Hyal-1 and Hyal-3 Immunohistochemistry Prognosis and morphometry.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100021

Evaluation of the antiedematogenic activity of artemetin isolated from Cordia curassavica DC
Provedor de dados: 56
Autores: Bayeux,M.C.; Fernandes,A.T.; Foglio,M.A.; Carvalho,J.E.
The species Cordia curassavica (Boraginaceae), known popularly as "erva baleeira", is used in folk medicine for the treatment of several inflammatory processes and as a healing agent. The objective of the present study was to evaluate the antiedematogenic activity of crude dichloromethane extracts of Cordia curassavica and of the artemetin-enriched fraction. The crude extract and artemetin fraction were tested in the model of carrageenin-induced paw edema in male Swiss mice (25-30 g). The crude dichloromethane extract (300 and 1000 mg/kg, po, N = 6) showed significant antiedematogenic activity, reducing the edema by 42, 57 and 45% and 46, 62 and 69%, 3, 4 and 5 h after carrageenin administration, respectively. Indomethacin (10 mg/kg, po, N = 6) reduced the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cordia curassavica; Cordia verbenaceae; Antiedematogenic; Anti-inflammatory; Medicinal plants.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000017

Correction
Provedor de dados: 56
Tipo: Info:eu-repo/semantics/report
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400013

Anti-parasitic action and elimination of intracellular Toxoplasma gondii in the presence of novel thiosemicarbazone and its 4-thiazolidinone derivatives
Provedor de dados: 56
Autores: Carvalho,C.S.; de Melo,E.J.T.
Toxoplasma, which infects all eukaryotic cells, is considered to be a good system for the study of drug action and of the behavior of infected host cells. In the present study, we asked if thiosemicarbazone derivatives can be effective against tachyzoites and which morphological and ultrastructural features of host cells and parasites are associated with the destruction of Toxoplasma. The compounds were tested in infected Vero cell culture using concentration screens (0.1 to 20 mM). The final concentration of 1 mM was chosen for biological assay. The following results were obtained: 1) These new derivatives decreased T. gondii infection with an in vitro parasite IC50% of 0.2-0.7 mM, without a significant effect on host cells and the more efficient...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acid [2-(benzylidene-hydrazone)-4-oxo-1; 3-thiazolidin-5-yl]acetic r-chloro-benzaldehyde thiosemicarbazone Tachyzoite Toxoplasma.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200004

Effect of aging on carotid artery stiffness and baroreflex sensitivity during head-out water immersion in man
Provedor de dados: 56
Autores: Ueno,L.M.; Miyachi,M.; Matsui,T.; Takahashi,K.; Yamazaki,K.; Hayashi,K.; Onodera,S.; Moritani,T.
To examine the possible age-related blood pressure (BP) deregulation in response to central hypervolemia, we measured spontaneous baroreflex sensitivity (SBRS), carotid arterial compliance (CC), and R-R interval coefficient of variation (RRICV) during basal and thermoneutral resting head-out-of-water immersion (HOWI) in 7 young (YG = 24.0 ± 0.8 years) and 6 middle-aged/older (OL = 59.3 ± 1.3 years) healthy men. Compared with basal conditions (YG = 19.6 ± 4.0 vs OL = 6.1 ± 1.5 ms/mmHg, P &lt; 0.05), SBRS remained higher in YG than OL during rest HOWI (YG = 23.6 ± 6.6 vs OL = 9.3 ± 2.1 ms/mmHg, P &lt; 0.05). The RRICV was significantly different between groups (YG = 6.5 ± 1.4 vs OL = 2.8 ± 0.4%, P &lt; 0.05) under HOWI. The OL group had no increase in CC,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Baroreflex sensitivity; Carotid compliance; Water immersion; Hypertension; Vascular stiffness.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400018

Use of flash glucose-sensing technology in patients with type 2 diabetes treated with liraglutide combined with CSII: a pilot study
Provedor de dados: 56
Autores: Yao,Ming-yan; Li,Li-qin; Ma,Jian-xia; Xue,Peng; Li,Yu-kun.
Glycemic variability (GV) may be linked to the development of diabetic complications by inducing inflammation, oxidative stress, and endothelial dysfunction. Flash glucose monitoring (FGM) provides a novel method of continuously monitoring interstitial glucose levels for up to 14 days. This study randomly assigned poorly controlled type 2 diabetes mellitus patients treated with metformin and multiple daily injections of insulin (n=60) to either continuous subcutaneous insulin infusion (CSII) treatment or CSII in combination with liraglutide (CSII+Lira) treatment for 14 days during hospitalization. GV was assessed using a FGM system; weight and cardiometabolic biomarkers were also evaluated. The coefficient of variation was significantly reduced in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flash glucose monitoring; Glucagon-like peptide-1 analog; Continuous subcutaneous insulin infusion; Glucose fluctuation; Cardiometabolic risk markers; Type 2 diabetes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100604

Expression of CYP2A3 mRNA and its regulation by 3-methylcholanthrene, pyrazole, and ß-ionone in rat tissues
Provedor de dados: 56
Autores: Robottom-Ferreira,A.B.; Aquino,S.R.; Queiroga,R.; Albano,R.M.; Ribeiro Pinto,L.F.
Cytochrome P450 (CYP) 2A enzymes are involved in the metabolism of numerous drugs and hormones and activate different carcinogens. Human CYP2A6, mouse CYP2A5 and rat CYP2A3 are orthologous enzymes that present high similarity in their amino acid sequence and share substrate specificities. However, different from the human and mouse enzyme, CYP2A3 is not expressed in the rat liver. There are limited data about expression of CYP2A3 in extrahepatic tissues and its regulation by typical CYP inducers. Therefore, the objective of the present study was to analyze CYP2A3 mRNA expression in different rat tissues by RT-PCR, and to study the influence of 3-methylcholanthrene, pyrazole and ß-ionone treatment on its expression. Male Wistar rats were divided into four...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytochrome P450; CYP2A3; Esophagus; Extrahepatic tissue.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700003

Comparative efficacy and safety of contact force-sensing catheter and second-generation cryoballoon ablation for atrial fibrillation: a meta-analysis
Provedor de dados: 56
Autores: Zhou,X.; Lv,W.; Zhang,W.; Ye,Y.; Li,Y.; Zhou,Q.; Zhang,J.; Xing,Q.; Lu,Y.; Zhang,L.; Wang,H.; Qin,W.; Tang,B.
This meta-analysis compared the efficacy and safety of the contact force (CF)-sensing catheter and second-generation cryoballoon (CB) ablation for treating atrial fibrillation (AF). Six controlled clinical trials comparing ablation for AF using a CF-sensing catheter or second-generation CB were identified from PubMed, EMBASE, Cochrane Library, Wanfang Data, and China National Knowledge Infrastructure. The procedure duration was significantly lower in the CB group compared with that in the CF group [mean difference (MD)=29.4; 95%CI=17.84–40.96; P=0.01], whereas there was no difference between the groups for fluoroscopy duration (MD=0.59; 95%CI=–4.48–5.66; P=0.82). Moreover, there was no difference in the incidence of non-lethal complications (embolic event,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atrial fibrillation; Ablation; Contact force-sensing catheter; Second-generation cryoballoon; Meta-analysis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900607

The left ventricular contractility of the rat heart is modulated by changes in flow and a1-adrenoceptor stimulation
Provedor de dados: 56
Autores: Vassallo,P.F.; Stefanon,I.; Rossoni,L.V.; Tucci,P.J.F.; Vassallo,D.V.
Myocardial contractility depends on several mechanisms such as coronary perfusion pressure (CPP) and flow as well as on &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;1-adrenoceptor stimulation. Both effects occur during the sympathetic stimulation mediated by norepinephrine. Norepinephrine increases force development in the heart and produces vasoconstriction increasing arterial pressure and, in turn, CPP. The contribution of each of these factors to the increase in myocardial performance needs to be clarified. Thus, in the present study we used two protocols: in the first we measured mean arterial pressure, left ventricular pressure and rate of rise of left ventricular pressure development in anesthetized rats (N = 10) submitted to phenylephrine (PE)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial performance; Coronary perfusion pressure; Coronary flow; Phenylephrine.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000019

Brain SPECT imaging in Sydenham's chorea
Provedor de dados: 56
Autores: Barsottini,O.G.P.; Ferraz,H.B.; Seviliano,M.M.; Barbieri,A.
The objective of the present study was to determine whether brain single-photon emission computed tomography (SPECT) imaging is capable of detecting perfusional abnormalities. Ten Sydenham's chorea (SC) patients, eight females and two males, 8 to 25 years of age (mean 13.4), with a clinical diagnosis of SC were submitted to brain SPECT imaging. We used HMPAO labeled with technetium-99m at a dose of 740 MBq. Six examinations revealed hyperperfusion of the basal ganglia, while the remaining four were normal. The six patients with abnormal results were females and their data were not correlated with severity of symptoms. Patients with abnormal brain SPECT had a more recent onset of symptoms (mean of 49 days) compared to those with normal SPECT (mean of 85...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sydenham's chorea; Single-photon emission computed tomography; SPECT imaging; Rheumatic fever; Brain perfusion.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400004

Precipitated immune complexes of IgM induce the generation of reactive oxygen species by rabbit polymorphonuclear leucocytes
Provedor de dados: 56
Autores: Lucisano,Y.M.; de-Mello,A.M.; Vasques,E.R.; Mantovani,B.
We report that immune complexes of IgM (ICIgM) antibodies and ovalbumin in the form of a precipitate from the equivalence zone induce the generation of reactive oxygen species by rabbit blood polymorphonuclear leucocytes (PMN), as measured by the chemiluminescence (CL) production in the presence of luminol. The kinetics of CL generation induced by ICIgM is quite different from that induced by precipitated immune complexes of IgG (ICIgG): the maximum rate of CL production for ICIgM occurs around 14 min, whereas for ICIgG it occurs about 5 min after incubation with the cells. Also the triggering of the process requires a higher concentration of ICIgM than of ICIgG. Evidence is presented that these effects are not mediated by interaction of the antigen...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Reactive oxygen species; Polymorphonuclear leucocytes; Immune complexes; IgM; IgG.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600011

Cultivation and identification of colon cancer stem cell-derived spheres from the Colo205 cell line
Provedor de dados: 56
Autores: Li,Ying-fei; Xiao,Bing; Tu,San-fang; Wang,Yuan-yuan; Zhang,Xiao-lang.
Our group established a method to culture spheres under serum-free culture condition. However, the biological characteristics and the tumorigenicity of spheres are unknown. Here, we demonstrate that sphere cells expressed high levels of the putative colorectal cancer stem cell markers CD133 and CD44. The CD133-positive rates were 13.27 ± 5.62, 52.71 ± 16.97 and 16.47 ± 2.45% in sphere cells, regular Colo205 cells and differentiated sphere cells, respectively, while the CD44-positive rates were 62.92 ± 8.38, 79.06 ± 12.10 and 47.80 ± 2.5%, respectively, and the CD133/CD44-double-positive rates were 10.77 ± 4.96, 46.89 ± 19.17 and 12.41 ± 2.27%, respectively (P &lt; 0.05). Cancer sphere cells formed crypt-like structures in 3-D culture. Moreover, cells from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colon cancer stem cells; Cancer sphere; Self-renew; Differentiation; Tumorigenicity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300003

Concentration of CCL11, CXCL8 and TNF-alpha in sputum and plasma of patients undergoing asthma or chronic obstructive pulmonary disease exacerbation
Provedor de dados: 56
Autores: Daldegan,M.B.; Teixeira,M.M.; Talvani,A.
Asthma and chronic obstructive pulmonary disease (COPD) are common respiratory illnesses characterized by chronic inflammation of the airways. The characterization of induced or spontaneously produced sputum is a useful technique to assess airway inflammation. In the present study, we compared the concentrations of CCL2, CCL11, CXCL8, and tumor necrosis factor-alpha (TNF-alpha) in plasma and induced sputum of patients with severe asthma or COPD and correlated the levels of these mediators with inflammatory cells in sputum. Asthmatic patients had elevated levels of eosinophils (40.1 ± 6.24%) in sputum whereas neutrophils (63.3 ± 4.66%) predominated in COPD patients. The levels of the chemokine CCL11 were markedly increased in sputum (708.7 ± 330.7 pg/ml)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Chronic obstructive pulmonary disease; Chemokines; TNF-alpha.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900010

Renin-angiotensin system blockers regulate the metabolism of isolated fat cells in vitro
Provedor de dados: 56
Autores: Caminhotto,R de O.; Sertié,R.A.L.; Andreotti,S.; Campaãa,A.B.; Lima,F.B.
Due to the presence of the renin-angiotensin system (RAS) in tissues and its specific influence on white adipose tissue, fat cells are possible targets of pharmacological RAS blockers commonly used as anti-hypertensive drugs. In the present study, we investigated the effects of different RAS blockers on fat cell metabolism, more specifically on lipolysis, lipogenesis and oxidation of energy substrates. Isolated primary adipocytes were incubated with different RAS blockers (aliskiren, captopril and losartan) in vitro for 24 h and lipolysis, lipogenesis and glucose oxidation capacities were determined in dose-response assays to a β-adrenergic agonist and to insulin. Although no change was found in lipolytic capacity, the RAS blockers modulated lipogenesis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipocytes; Lipolysis; Lipogenesis; Glucose; Renin-angiotensin system.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800608

Potential pathogenic role of aggregative- adhering Corynebacterium diphtheriae of different clonal groups in endocarditis
Provedor de dados: 56
Autores: Hirata Jr.,R.; Pereira,G.A.; Filardy,A.A.; Gomes,D.L.R.; Damasco,P.V.; Rosa,A.C.P.; Nagao,P.E.; Pimenta,F.P.; Mattos-Guaraldi,A.L.
Invasive diseases caused by Corynebacterium diphtheriae have been described increasingly. Several reports indicate the destructive feature of endocarditis attributable to nontoxigenic strains. However, few reports have dealt with the pathogenicity of invasive strains. The present investigation demonstrates a phenotypic trait that may be used to identify potentially invasive strains. The study also draws attention to clinical and microbiological aspects observed in 5 cases of endocarditis due to C. diphtheriae that occurred outside Europe. Four cases occurred in female school-age children (7-14 years) treated at different hospitals in Rio de Janeiro, Brazil. All patients developed other complications including septicemia, renal failure and/or arthritis....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aggregative adherence; Corynebacterium diphtheriae; Endocarditis; HEp-2 cells; Random amplified polymorphic DNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100007

Human papillomavirus detection and p16 methylation pattern in a case of esophageal papilloma
Provedor de dados: 56
Autores: Afonso,L.A.; Moysés,N.; Cavalcanti,S.M.B.
Esophageal cancer is a prevalent cancer worldwide. Some studies have reported the possible etiology of human papillomavirus (HPV) in benign and malignant papillomas of the esophagus but the conclusions are controversial. In the present study, we investigated an esophageal papilloma from a 30-year-old male patient presenting aphasia. HPV DNA was detected by generic PCR using MY09/11 primers, and restriction fragment length polymorphism revealed the presence of HPV54, usually associated with benign genital lesions. Hypermethylation of the pINK4A gene was also investigated due to its relation to malignant transformation, but no modification was detected in the host gene. Except for an incipient reflux, no risk factors such as cigarette smoking, alcohol abuse...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Human papillomavirus; Esophageal cancer; Restriction fragment length polymorphism.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700013

MicroRNA-455 suppresses the oncogenic function of HDAC2 in human colorectal cancer
Provedor de dados: 56
Autores: Mao,Q.D.; Zhang,W.; Zhao,K.; Cao,B.; Yuan,H.; Wei,L.Z.; Song,M.Q.; Liu,X.S.
Colorectal cancer (CRC) is the fourth leading cause of cancer-induced mortality. Histone deacetylase 2 (HDAC2) is involved in prognosis and therapy of CRC. This study aimed to explore novel therapeutic targets for CRC. The alteration of HDAC2 expression in CRC tissues was estimated by qRT-PCR. After lentivirus transfection, HDAC2 knockdown was confirmed by western blot analysis. The effect of HDAC2 knockdown on cell proliferation was then assessed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Screened by TargetScan, microRNA (miR)-455 was predicted to bind to 3′UTR of HDAC2 and the prediction was verified by luciferase assay. Finally, cells were transfected, respectively, with miR-455 mimics or miR-455 negative control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colorectal cancer; Histone deacetylase 2; MiR-455; Proliferation; Apoptosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600608

The relationship between anxiety and depression in animal models: a study using the forced swimming test and elevated plus-maze
Provedor de dados: 56
Autores: Andreatini,R.; Bacellar,L.F.S.
The present study evaluated the correlation between the behavior of mice in the forced swimming test (FST) and in the elevated plus-maze (PM). The effect of the order of the experiments, i.e., the influence of the first test (FST or PM) on mouse behavior in the second test (PM or FST, respectively) was compared to handled animals (HAND). The execution of FST one week before the plus-maze (FST-PM, N = 10), in comparison to mice that were only handled (HAND-PM, N = 10) in week 1, decreased % open entries (HAND-PM: 33.6 ± 2.9; FST-PM: 20.0 ± 3.9; mean ± SEM; P&amp;lt;0.02) and % open time (HAND-PM: 18.9 ± 3.3; FST-PM: 9.0 ± 1.9; P&amp;lt;0.03), suggesting an anxiogenic effect. No significant effect was seen in the number of closed arm entries (FST-PM: 9.5...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Anxiety; Depression; Comorbidity; Animal models.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900011

A randomized, double-blind, and placebo-controlled study with tranexamic acid of bleeding and fibrinolytic activity after primary coronary artery bypass grafting
Provedor de dados: 56
Autores: Santos,A.T.L.; Kalil,R.A.K.; Bauemann,C.; Pereira,J.B.; Nesralla,I.A.
Cardiopulmonary bypass is frequently associated with excessive blood loss. Platelet dysfunction is the main cause of non-surgical bleeding after open-heart surgery. We randomized 65 patients in a double-blind fashion to receive tranexamic acid or placebo in order to determine whether antifibrinolytic therapy reduces chest tube drainage. The tranexamic acid group received an intravenous loading dose of 10 mg/kg, before the skin incision, followed by a continuous infusion of 1 mg kg-1 h-1 for 5 h. The placebo group received a bolus of normal saline solution and continuous infusion of normal saline for 5 h. Postoperative bleeding and fibrinolytic activity were assessed. Hematologic data, convulsive seizures, allogeneic transfusion, occurrence of myocardial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tranexamic acid; Coronary artery bypass; Postoperative bleeding; Clinical trial.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000100007

Follow-up of patients treated with retinoic acid for the control of radioiodine non-responsive advanced thyroid carcinoma
Provedor de dados: 56
Autores: Coelho,S.M.; Vaisman,F.; Buescu,A.; Mello,R.C.R.; Carvalho,D.P.; Vaisman,M.
During thyroid tumor progression, cellular de-differentiation may occur and it is commonly accompanied by metastatic spread and loss of iodine uptake. Retinoic acid (RA) administration might increase iodine uptake in about 40% of patients, suggesting that RA could be a promising therapeutic option for radioiodine non-responsive thyroid carcinoma, although a prospective study with a long-term follow-up has not been reported. This was a clinical prospective study assessing the value of 13-cis-RA in patients with advanced thyroid carcinoma and its impact on major outcomes such as tumor regression and cancer-related death with a long-term follow-up of patients submitted to radioiodine (131I) therapy after RA administration. Sixteen patients with inoperable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thyroid cancer; Retinoic acid; Radioiodine therapy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100011

Serum total bilirubin levels are negatively correlated with metabolic syndrome in aged Chinese women: a community-based study
Provedor de dados: 56
Autores: Zhong,P.; Sun,D.M.; Wu,D.H.; Li,T.M.; Liu,X.Y.; Liu,H.Y.
We evaluated serum total bilirubin levels as a predictor for metabolic syndrome (MetS) and investigated the relationship between serum total bilirubin levels and MetS prevalence. This cross-sectional study included 1728 participants over 65 years of age from Eastern China. Anthropometric data, lifestyle information, and previous medical history were collected. We then measured serum levels of fasting blood-glucose, total cholesterol, triglycerides, and total bilirubin, as well as alanine aminotransferase activity. The prevalence of MetS and each of its individual component were calculated per quartile of total bilirubin level. Logistic regression was used to assess the correlation between serum total bilirubin levels and MetS. Total bilirubin level in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Total bilirubin; Metabolic syndrome; Oxidative stress.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000200701

Increased coherence among striatal regions in the theta range during attentive wakefulness
Provedor de dados: 56
Autores: Lepski,G.; Arévalo,A.; Valle,A.C. do; Ballester,G.; Gharabaghi,A.
The striatum, the largest component of the basal ganglia, is usually subdivided into associative, motor and limbic components. However, the electrophysiological interactions between these three subsystems during behavior remain largely unknown. We hypothesized that the striatum might be particularly active during exploratory behavior, which is presumably associated with increased attention. We investigated the modulation of local field potentials (LFPs) in the striatum during attentive wakefulness in freely moving rats. To this end, we implanted microelectrodes into different parts of the striatum of Wistar rats, as well as into the motor, associative and limbic cortices. We then used electromyograms to identify motor activity and analyzed the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attention; Behavior; Striatum; Theta rhythm; Partial directed coherence.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800011

Pulmonary vein parameters are similar or better predictors than left atrial diameter for paroxysmal atrial fibrillation after cryoablation
Provedor de dados: 56
Autores: Li,Bolin; Ma,Honglan; Guo,Huihui; Liu,Peng; Wu,Yue; Fan,Lihong; Cao,Yumeng; Jian,Zhijie; Sun,Chaofeng; Li,Hongbing.
Left atrial diameter (LAD) has been considered an independent risk factor for atrial fibrillation (AF) relapse after pulmonary vein isolation (PVI). However, whether LAD or other factors are more predictive of late recurrence in patients with paroxysmal AF remains unclear. We aimed to evaluate the value of pulmonary vein (PV) parameters for predicting AF relapse 1 year after patients underwent cryoablation for paroxysmal AF. Ninety-seven patients with paroxysmal AF who underwent PVI successfully were included. PV parameters were measured through computed tomography scans prior to PVI. A total of 28 patients had recurrence of AF at one-year follow-up. The impact of several variables on recurrence was evaluated in multivariate analyses. LAD and the time from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atrial fibrillation; Cryoablation; Right inferior pulmonary vein; Pulmonary vein parameter.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900610

Ventilation-induced changes correlate to pulmonary vascular response and VEGF, VEGFR-1/2, and eNOS expression in the rat model of postnatal hypoxia
Provedor de dados: 56
Autores: Figueira,R.L.; Gonçalves,F.L.; Prado,A.R.; Ribeiro,M.C.; Costa,K.M.; Silva,O. Castro e; Sbragia,L.
Neonatal asphyxia occurs due to reduction in oxygen supply to vital organs in the newborn. Rapid restoration of oxygen to the lungs after a long period of asphyxia can cause lung injury and decline of respiratory function, which result from the activity of molecules that induce vascular changes in the lung such as nitric oxide (NO) and vascular endothelial growth factors (VEGF). In this study, we evaluated the pulmonary and vascular morphometry of rats submitted to the model of neonatal asphyxia and mechanical ventilation, their expression of pulmonary VEGF, VEGF receptors (VEGFR-1/VEGFR-2), and endothelial NO synthase (eNOS). Neonate Sprague-Dawley rats (CEUA #043/2011) were divided into four groups (n=8 each): control (C), control submitted to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neonatal hypoxia; Mechanical ventilation; VEGF; VEGF receptors; ENOS enzyme.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100608

Inhibitory effect of liposomal quercetin on acute hepatitis and hepatic fibrosis induced by concanavalin A
Provedor de dados: 56
Autores: Wan,Y.; Tang,M.H.; Chen,X.C.; Chen,L.J.; Wei,Y.Q.; Wang,Y.S.
Immune response plays an important role in the development of hepatic fibrosis. In the present study, we investigated the effects of quercetin on hepatitis and hepatic fibrosis induced by immunological mechanism. In the acute hepatitis model, quercetin (2.5 mg/kg) was injected iv into mice 30 min after concanavalin A (Con A) challenge. Mice were sacrificed 4 or 24 h after Con A injection, and aminotransferase tests and histopathological sections were performed. Treatment with quercetin significantly decreased the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Consistent with this observation, treatment with quercetin markedly attenuated the pathologic changes in the liver. A hepatic fibrosis model was also generated in mice...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quercetin; Concanavalin A; Acute hepatitis; Hepatic fibrosis; Liposome.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000800655

An MD-PhD program in Brazil: students’ concepts of science and of common sense
Provedor de dados: 56
Autores: Oliveira,R.V.; Campos,P.C.C.; Mourão,P.A.S.
In 1995, a pioneering MD-PhD program was initiated in Brazil for the training of medical scientists in experimental sciences at the Federal University of Rio de Janeiro. The program’s aim was achieved with respect to publication of theses in the form of papers with international visibility and also in terms of fostering the scientific careers of the graduates. The expansion of this type of program is one of the strategies for improving the preparation of biomedical researchers in Brazil. A noteworthy absence of interest in carrying out clinical research limits the ability of young Brazilian physicians to solve biomedical problems. To understand the students’ views of science, we used qualitative and quantitative triangulation methods, as well as...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: MD-PhD program evaluation; Concept of science; Common sense concept; Medical research training; Methodological triangulation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100005

Allantoin as an independent marker associated with carotid intima-media thickness in subclinical atherosclerosis
Provedor de dados: 56
Autores: Santana,M.S.; Nascimento,K.P.; Lotufo,P.A.; Benseñor,I.M.; Meotti,F.C.
Allantoin is the main product of uric acid oxidation and was found to be augmented in atherosclerotic plaque in human autopsy and in animal models of atherosclerosis. Uric acid is abundant in human plasma and is prone to oxidation in inflammatory conditions such as atherosclerosis. In this study, we found a significant increase in plasma uric acid (P=0.002) and allantoin (P=0.025) in participants of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) that presented common carotid intima-media thickness (c-IMT) within the 75th percentile (c-IMT≥P75). Multiple linear regression showed an association of c-IMT with uric acid (β=0.0004, P=0.014) and allantoin (β=0.018, P=0.008). This association was independent of age, the traditional risk factor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allantoin; Uric acid; Common carotid intima-media thickness; Atherosclerosis; Oxidation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800613

The role of the vagus nerve in hypertonic resuscitation of hemorrhagic shocked dogs
Provedor de dados: 56
Autores: Velasco,I.T.; Baena,R.C.
Previous studies have suggested a critical role for the vagi during the hypertonic resuscitation of hemorrhagic shocked dogs. Vagal blockade prevented the full hemodynamic and metabolic recovery and increased mortality. This interpretation, however, was challenged on the grounds that the blockade also abolished critical compensatory mechanisms and therefore the animals would die regardless of treatment. To test this hypothesis, 29 dogs were bled (46.0 ± 6.2 ml/kg, enough to reduce the mean arterial pressure to 40 mmHg) and held hypotensive for 45 min. After 40 min, vagal activity was blocked in a reversible manner (0ºC/15 min) and animals were resuscitated with 7.5% NaCl (4 ml/kg), 0.9% NaCl (32 ml/kg), or the total volume of shed blood. In the vagal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertonic resuscitation; Hypertonic sodium chloride; Hemorrhagic shock; Plasma volume.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300020

Antioxidant status of dog aqueous humor after extracapsular lens extraction
Provedor de dados: 56
Autores: Barros,P.S.M.; Padovani,C.F.; Silva,V.V.; Queiroz,L.; Barros,S.B.M.
We determined the antioxidant status of the aqueous humor after extracapsular lens extraction in 14 mongrel dogs weighing about 10 kg. The animals were examined by slit lamp biomicroscopy, applanation tonometry and indirect ophthalmoscopy. One eye was submitted to conventional extracapsular lens extraction and the other was used as control. Samples of aqueous humor were obtained by anterior chamber paracentesis before and at days 1, 2, 3, 7 and 15 after surgery. Total antioxidant status was determined as the capacity of aqueous humor to inhibit free radical generation by 2,2-azobis(2-amidopropane) chlorine. Ascorbic acid concentration was measured by HPLC with UV detection. Protein content was determined with the biuret reagent. Statistical analysis was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lens; Aqueous humor; Oxidative stress; Antioxidant; Extracapsular lens extraction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100007

Inhibition of miR-383 suppresses oxidative stress and improves endothelial function by increasing sirtuin 1
Provedor de dados: 56
Autores: Hu,Baoxiang; Gong,Zushun; Bi,Zhaohui.
Previous research has shown that suppression of miR-383 can prevent inflammation of the endothelium, as well as postpone the development of atherosclerosis. However, the role of miR-383 in endothelial cell apoptosis in diabetes remains unclear. The aim of this study was to investigate the function of miR-383 in high glucose-induced apoptosis and oxidative stress in endothelial cells. A series of experiments involving qualitative polymerase chain reaction, cell transfection, luciferase assay, assessment of cell death, detection of catalase and superoxide dismutase concentrations, detection of intracellular reactive oxygen species (ROS), and western blot analysis were performed in this study. We found that miR-383 expression was promoted, while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-383; Oxidative stress; Human umbilical vein endothelial cells; Sirtuin 1.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200603

Relationship between the quality of life and the severity of obstructive sleep apnea syndrome
Provedor de dados: 56
Autores: Lopes,C.; Esteves,A.M.; Bittencourt,L.R.A.; Tufik,S.; Mello,M.T.
The effects of sleep disorders on the quality of life (QOL) have been documented in the literature. Excessive sleepiness and altered circadian rhythms may negatively affect ability to learn, employment, and interpersonal relations, and directly degrade QOL. The objective of the present study was to evaluate the impact of obstructive sleep apnea syndrome of varying severity on QOL. The study was conducted on 1892 patients aged 18 years or older referred by a physician to the Sleep Institute, São Paulo, with complaints related to apnea (snoring, excessive daytime sleepiness, hyperarousal, and fatigue). They were submitted to overnight polysomnography for the diagnosis of sleep disorders from August 2005 through April 2006. The patients completed the Epworth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physical activity; Obstructive sleep apnea syndrome; Quality of life; Sleepiness.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000013

A protein with amino acid sequence homology to bovine insulin is present in the legume Vigna unguiculata (cowpea)
Provedor de dados: 56
Autores: Venâncio,T.M.; Oliveira,A.E.A.; Silva,L.B.; Machado,O.L.T.; Fernandes,K.V.S.; Xavier-Filho,J.
Since the discovery of bovine insulin in plants, much effort has been devoted to the characterization of these proteins and elucidation of their functions. We report here the isolation of a protein with similar molecular mass and same amino acid sequence to bovine insulin from developing fruits of cowpea (Vigna unguiculata) genotype Epace 10. Insulin was measured by ELISA using an anti-human insulin antibody and was detected both in empty pods and seed coats but not in the embryo. The highest concentrations (about 0.5 ng/µg of protein) of the protein were detected in seed coats at 16 and 18 days after pollination, and the values were 1.6 to 4.0 times higher than those found for isolated pods tested on any day. N-terminal amino acid sequencing of insulin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Insulin-like; Cowpea; Vigna unguiculata; Developing fruits; Sequence homology; Evolution.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900004

Interleukin 8 as a vaso-occlusive marker in Brazilian patients with sickle cell disease
Provedor de dados: 56
Autores: Gonçalves,M.S.; Queiroz,I.L.; Cardoso,S.A.; Zanetti,A.; Strapazoni,A.C.; Adorno,E.; Albuquerque,A.; Sant'Ana,A.; Reis,M.G. dos; Barral,A.; Barral Netto,M.
Sickle cell disease has a worldwide distribution and is a public health problem in Brazil. Although vaso-occlusive crisis (VOC) is one of the most important clinical features of the disease, there are still several steps of its pathogenesis which are unknown. The increase of the chemotactic factor interleukin 8 (IL-8) has been reported to be involved in sickle cell disease crisis, but this has not been demonstrated conclusively. In the present study we analyzed serum IL-8 levels by ELISA and hematological parameters and hemoglobin patterns by standard techniques in 23 (21 SS and 2 SC) Brazilian patients with sickle cell syndromes during VOC caused by different inducing factors, 22 (21 SS and 1 SC) sickle cell patients out of crisis, and 11 healthy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interleukin 8; Sickle cell syndromes; Vaso-occlusive crisis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000011

Relationships between fetal body weight of Wistar rats at term and the extent of skeletal ossification
Provedor de dados: 56
Autores: Chahoud,I.; Paumgartten,F.J.R.
We investigated the relationship between fetal body weight at term (pregnancy day 21) and the extent of ossification of sternum, metacarpus, metatarsus, phalanges (proximal, medial and distal) of fore- and hindlimbs and cervical and coccygeal vertebrae in Wistar rats. The relationships between fetal body weight and sex, intrauterine position, uterine horn, horn size, and litter size were determined using historical control data (7594 fetuses; 769 litters) of untreated rats. Relationships between body weight and degree of ossification were examined in a subset of 1484 historical control fetuses (154 litters) which were subsequently cleared and stained with alizarin red S. Fetal weight was independent of horn size, uterine horn side (left or right) or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ossification centers; Fetal skeleton; Delayed fetal growth; Prenatal growth retardation; Malformation; Embryotoxicity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400010

Impact of extracellular alkalinization on the survival of human CD24-/CD44+ breast cancer stem cells associated with cellular metabolic shifts
Provedor de dados: 56
Autores: Wanandi,S.I.; Yustisia,I.; Neolaka,G.M.G.; Jusman,S.W.A.
Cancer stem cells reside in a distinct region within the tumor microenvironment that it is believed to play a fundamental role in regulating stemness, proliferation, survival, and metabolism of cancer cells. This study aimed to analyze the effect of extracellular alkalinization on metabolism and survival of human CD24-/CD44+ breast cancer stem cells (BCSCs). BCSCs were cultured in alkalinized DMEM-F12 and incubated at 37°C, 5% CO2, and 20% O2 for 30 min, 6, 24, and 48 h. After each incubation period, we analyzed the modulation of various mRNA expressions related to pH and cellular metabolic regulation using the qRT-PCR. Metabolic state was measured using colorimetric and fluorometric assays. To examine cell proliferation and apoptosis, we used trypan blue...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular alkalinization; Metabolic states; Breast cancer stem cells; Cell survival; NaHCO3.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800609

Comments on the paper "High doses of riboflavin and the elimination of dietary red meat promote the recovery of some motor functions in Parkinson's disease patients. C.G. Coimbraand V.B.C. Junqueira. Brazilian Journal of Medical and Biological Research, 36: 1409-1417, 2003"
Provedor de dados: 56
Autores: Ferraz,H.B.; Quagliato,E.A.B.; Rieder,C.R.M.; Silva,D.J.; Teive,H.A.G.; Barbosa,E.R.; Cardoso,F.; Limongi,J.C.P.; Bezerra,J.M.F.; Andrade,L.A.F.; Allam,N.; Prado,R.C.P.; Tomas,V.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Parkinson's disease; Riboflavin; FAD; Glutathione; Iron; Hemin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900002

Purification of human albumin by the combination of the method of Cohn with liquid chromatography
Provedor de dados: 56
Autores: Tanaka,K.; Shigueoka,E.M.; Sawatani,E.; Dias,G.A.; Arashiro,F.; Campos,T.C.X.B.; Nakao,H.C.
Large volumes of plasma can be fractionated by the method of Cohn at low cost. However, liquid chromatography is superior in terms of the quality of the product obtained. In order to combine the advantages of each method, we developed an integrated method for the production of human albumin and immunoglobulin G (IgG). The cryoprecipitate was first removed from plasma for the production of factor VIII and the supernatant of the cryoprecipitate was fractionated by the method of Cohn. The first precipitate, containing fractions (F)-I + II + III, was used for the production of IgG by the chromatographic method (see Tanaka K et al. (1998) Brazilian Journal of Medical and Biological Research, 31: 1375-1381). The supernatant of F-I + II + III was submitted to a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Albumin production; Industrial chromatography; Hemoderivate production; Downstream process; Plasma derivatives.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100003

Some physico-chemical parameters that influence proteinase K resistance and the infectivity of PrP Sc after high pressure treatment
Provedor de dados: 56
Autores: Heindl,P.; Fernández García,A.; Büttner,M.; Voigt,H.; Butz,P.; Tauscher,B.; Pfaff,E.
Crude brain homogenates of terminally diseased hamsters infected with the 263 K strain of scrapie (PrP Sc) were heated and/or pressurized at 800 MPa at 60ºC for different times (a few seconds or 5, 30, 120 min) in phosphate-buffered saline (PBS) of different pH and concentration. Prion proteins were analyzed on immunoblots for their proteinase K (PK) resistance, and in hamster bioassays for their infectivity. Samples pressurized under initially neutral conditions and containing native PrP Sc were negative on immunoblots after PK treatment, and a 6-7 log reduction of infectious units per gram was found when the samples were pressurized in PBS of pH 7.4 for 2 h. A pressure-induced change in the protein conformation of native PrP Sc may lead to less PK...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Native prion protein; Pressure; Inactivation; PH; Proteinase K sensitivity; Prion conformation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800010

Determination of renal function in long-term heart transplant patients by measurement of urinary retinol-binding protein levels
Provedor de dados: 56
Autores: Chinen,R.; Câmara,N.O.S.; Nishida,S.; Silva,M.S.; Rodrigues,D.A.; Pereira,A.B.; Pacheco-Silva,A.
Significant improvements have been noted in heart transplantation with the advent of cyclosporine. However, cyclosporine use is associated with significant side effects, such as chronic renal failure. We were interested in evaluating the incidence of long-term renal dysfunction in heart transplant recipients. Fifty-three heart transplant recipients were enrolled in the study. Forty-three patients completed the entire evaluation and follow-up. Glomerular (serum creatinine, creatinine clearance measured, and creatinine clearance calculated) and tubular functions (urinary retinol-binding protein, uRBP) were re-analyzed after 18 months. At the enrollment time, the prevalence of renal failure ranged from 37.7 to 54% according to criteria used to define it...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal function; Urinary retinol-binding protein; Heart transplant; Graft survival; Chronic renal failure.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001000006

The effects of stretching on the flexibility, muscle performance and functionality of institutionalized older women
Provedor de dados: 56
Autores: Gallon,D.; Rodacki,A.L.F.; Hernandez,S.G.; Drabovski,B.; Outi,T.; Bittencourt,L.R.; Gomes,A.R.S.
Stretching has been widely used to increase the range of motion. We assessed the effects of a stretching program on muscle-tendon length, flexibility, torque, and activities of daily living of institutionalized older women. Inclusion/exclusion criteria were according to Mini-Mental State Examination (MMSE) (&gt;13), Barthel Index (&gt;13) and Lysholm Scoring Scale (&gt;84). Seventeen 67 ± 9 year-old elderly women from a nursing home were divided into 2 groups at random: the control group (CG, N = 9) participated in enjoyable cultural activities; the stretching group (SG, N = 8) performed active stretching of hamstrings, 4 bouts of 1 min each. Both groups were supervised three times per week over a period of 8 weeks. Peak torque was assessed by an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homes for the aged; Muscle stretching exercises; Muscle strength; Flexibility; Activities of daily living.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300009

In vitro-induced antibody production in chronic hepatitis C virus infection
Provedor de dados: 56
Autores: Oliveira Jr.,E.B.; Ferraz,M.L.G.; Perez,R.M.; Silva,A.E.B.; Lanzoni,V.P.; Granato,C.F.
The objectives of the present study were to assess the in vitro-induced anti-hepatitis C virus (HCV) antibody production (IVIAP) in relation to the clinical, biochemical, virologic and histologic variables of patients with HCV infection. The study included 57 patients (60% males) with HCV infection (anti-HCV and HCV-RNA positive). Alanine aminotransferase (ALT) was elevated in 89% of the patients. Mean viral load was 542,241 copies/ml and histology of the liver showed chronic hepatitis in 27/52 (52%) and cirrhosis in 11/52 (21%) patients. IVIAP levels were determined by immunoenzymatic assay at median absorbance of 0.781 at 450 nm. IVIAP was negative in 14% of the patients. When groups with IVIAP levels above and below the median were compared, high IVIAP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; In vitro-induced antibody production; IVIAP; Hepatitis C virus-RNA; Liver biopsy; Anti-HCV antibodies.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300011

Rarity of DNA sequence alterations in the promoter region of the human androgen receptor gene
Provedor de dados: 56
Autores: Cabral,D.F.; Santos,A.; Ribeiro,M.L.; Mesquita,J.C.; Carvalho-Salles,A.B.; Hackel,C.
The human androgen receptor (AR) gene promoter lies in a GC-rich region containing two principal sites of transcription initiation and a putative Sp1 protein-binding site, without typical "TATA" and "CAAT" boxes. It has been suggested that mutations within the 5'untranslated region (5'UTR) may contribute to the development of prostate cancer by changing the rates of gene transcription and/or translation. In order to investigate this question, the aim of the present study was to search for the presence of mutations or polymorphisms at the AR-5'UTR in 92 prostate cancer patients, where histological diagnosis of adenocarcinoma was established in specimens obtained from transurethral resection or after prostatectomy. The AR-5'UTR was amplified by PCR from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human androgen receptor gene; Promoter region; Prostate cancer; 5'Untranslated region; DNA sequence alterations.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200004

Pros and cons of insulin administration on liver glucose metabolism in strength-trained healthy mice
Provedor de dados: 56
Autores: Pereira,V.A.R.; Vedovelli,K.S.; Muller,G.Y.; Depieri,Y.F.; Avelar,D.H.C.G.; de Amo,A.H.E.; Jimenes,D.R.; Martins,J.N.L.; Silvério,A.C.; Gomes,C.R.G.; Godoi,V.A.F.; Pedrosa,M.M.D.
Non-diabetic individuals use hormones like insulin to improve muscle strength and performance. However, as insulin also leads the liver and the adipose tissue to an anabolic state, the purpose of this study was to investigate the effects of insulin on liver metabolism in trained non-diabetic Swiss mice. The mice were divided into four groups: sedentary treated with saline (SS) or insulin (SI) and trained treated with saline (TS) or insulin (TI). Training was made in a vertical stair, at 90% of the maximum load, three times per week. Insulin (0.3 U/kg body weight) or saline were given intraperitoneally five times per week. After eight weeks, tissue and blood were collected and in situ liver perfusion with glycerol+lactate or alanine+glutamine (4 mM each)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscle strength; Gluconeogenesis; Liver; Adiposity; Training.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200605

Gastroesophageal reflux episodes in asthmatic patients and their temporal relation with sleep architecture
Provedor de dados: 56
Autores: Mello-Fujita,L.; Roizenblat,S.; Frison.,C.R.; Rodrigues Junior,L.; Garbuio,S.; Tufik,S.; Bittencourt,L.R.A.
Gastroesophageal reflux (GER) is common in asthma patients and can contribute to sleep disruption. The aim of the present study was to determine the time-related distribution of GER events together with their impact on sleep in asthmatic subjects with GER disease symptoms. The inclusion criteria were: 18-65 years, controlled moderate to severe asthma and GER-compatible clinical evidence. The exclusion criteria were: chronic obstructive lung disease, smoking, infections of the upper airways, use of oral corticosteroids, other co-morbidities, pregnancy, sleep-related disorders, night-time shift work, and the use of substances with impact on sleep. Asthmatic patients with nocturnal symptoms were excluded. All-night polysomnography and esophageal pH monitoring...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Gastroesophageal reflux; Polysomnography; Sleep.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200012

Morphometric analysis of the phrenic nerve in male and female Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR)
Provedor de dados: 56
Autores: Rodrigues,A.R.; Ferreira,R.S.; Salgado,H.C.; Fazan,V.P.S.
Ventilatory differences between rat strains and genders have been described but the morphology of the phrenic nerve has not been investigated in spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats. A descriptive and morphometric study of the phrenic nerves of male (N = 8) and female (N = 9) SHR, and male (N = 5) and female (N = 6) WKY is presented. After arterial pressure and heart rate recordings, the phrenic nerves of 20-week-old animals were prepared for epoxy resin embedding and light microscopy. Morphometric analysis performed with the aid of computer software that took into consideration the fascicle area and diameter, as well as myelinated fiber profile and Schwann cell nucleus number per area. Phrenic nerves were generally...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phrenic nerve; Sexual dimorphism; Morphometry; Spontaneously hypertensive rats; Wistar-Kyoto rats; Asymmetry.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600012

Chronic lactose intake modifies the gastric emptying of monosaccharides but not of disaccharides in weanling rats
Provedor de dados: 56
Ninety-six weanling male Wistar rats were fed for four weeks one of two different chows: a normal rat chow containing 55.5% (w/w) starch (control group, N = 48) or a rat chow in which starch was partially replaced by lactose, in such a way that the experimental group (N = 48) received 35.5% (w/w) starch and 20% (w/w) lactose. The gastric emptying of fluid was then studied by measuring the gastric retention of four test meals containing lactose (5% or 10%, w/v) or glucose + galactose (5% or 10%, w/v). Homogenates of the small intestine were assayed for lactase activity. The gastric retention values were obtained 15 min after orogastric infusion of the liquid meals. The median values for gastric retention of the 5% lactose solutions were 37.7% for the...
Palavras-chave: Gastric emptying; Intestinal motility; Lactase; Lactose.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600004

Is avoidant disorder part of the social phobia spectrum in a referred sample of Brazilian children and adolescents?
Provedor de dados: 56
Autores: Denardin,D.; Silva,T.L.; Pianca,T.G.; Rohde,L.A.
The diagnosis of avoidant disorder was deleted from the Diagnostic and Statistical Manual of Mental disorders - fourth edition (DSM-IV) based on a `committee decision' suggesting that avoidant disorder is part of the social phobia spectrum. The objective of the present study was to examine the nature of this clinical association in a referred sample of Brazilian children and adolescents. We assessed a referred sample of 375 youths using semi-structured diagnostic interview methodology. Demographic (age at admission to the study and sex) and clinical (level of impairment, age at onset of symptoms and pattern of comorbidity) data were assessed in subsamples of children with avoidant disorder (N = 7), social phobia (N = 26), and comorbidity between both...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Anxiety disorders; Avoidant disorder; Social phobia; Mental disorder diagnosis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600012

Serodiagnosis of Helicobacter pylori infection by Cobas Core ELISA in adults from Minas Gerais, Brazil
Provedor de dados: 56
Autores: Rocha,G.A.; Oliveira,A.M.R.; Queiroz,D.M.M.; Mendes,E.N.; Moura,S.B.; Oliveira,C.A.; Ferrari,T.C.A.
We evaluated the accuracy of a 2nd generation ELISA to detect Helicobacter pylori infection in adults from a developing country in view of variations in sensitivity and specificity reported for different populations. We studied 97 non-consecutive patients who underwent endoscopy for evaluation of dispeptic symptoms. The presence of H. pylori was determined in antral biopsy specimens by culture, by the preformed urease test and in carbolfuchsin-stained smears. Patients were considered to be H. pylori positive if at least two of the three tests presented a positive result or if the culture was positive, and negative if the three tests were negative. Sixty-five adults (31 with peptic ulcer) were H. pylori positive and 32 adults were H. pylori negative....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Serodiagnosis; ELISA.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000005

Pancreatic β-cell function is inhibited by miR-3666 in type 2 diabetes mellitus by targeting adiponectin
Provedor de dados: 56
Autores: Tan,J.; Tong,A.; Xu,Y.
Type 2 diabetes mellitus (T2D) is a common endocrine and metabolic disorder, and poses threats to human health worldwide. Recently, microRNAs (miRNAs) have been suggested to play important roles in the pathophysiology of T2D. In this study, we explored the role of miR-3666 in T2D. miR-3666 was significantly down-regulated in the serum of T2D patients when compared to that of healthy volunteers, and miR-3666 expression level was negatively correlated with blood glucose levels of T2D patients. Overexpression of miR-3666 inhibited cell proliferation, reduced insulin secretion, and promoted cell apoptosis of pancreatic β-cell line (INS-1 cells). On the other hand, knockdown of miR-3666 had the opposite effects in INS-1 cells. The bio-informatics analysis using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes mellitus; MiR-3666; Insulin secretion; Cell proliferation; ADIPOQ.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600607

Rare adverse events associated with oral poliovirus vaccine in Brazil
Provedor de dados: 56
Oral poliovirus vaccine (OPV) developed by A. Sabin has been effectively used to control poliomyelitis in Brazil, and the last case with the isolation of a wild poliovirus strain occurred in March 1989. Although the vaccine controlled the circulation of wild strains and poliomyelitis cases associated with these strains were not detected during the last eight years, rare cases classified as vaccine-associated paralytic poliomyelitis (VAPP) have been detected. Molecular characterization studies of poliovirus strains isolated from VAPP cases and from healthy contacts have confirmed that the isolates are derived from the Sabin vaccine strains and also detected genomic modifications known or suspected to increase neurovirulence such as mutations and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Poliovirus; Poliomyelitis; Guillain-Barré syndrome; Transverse myelitis; Facial paralysis; Vaccine-associated cases.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600001

Detection of acute cerebral hemorrhage in rabbits by magnetic induction
Provedor de dados: 56
Autores: Sun,J.; Jin,G.; Qin,M.X.; Wan,Z.B.; Wang,J.B.; Wang,C.; Guo,W.Y.; Xu,L.; Ning,X.; Xu,J.; Pu,X.J.; Chen,M.S.; Zhao,H.M.
Acute cerebral hemorrhage (ACH) is an important clinical problem that is often monitored and studied with expensive devices such as computed tomography, magnetic resonance imaging, and positron emission tomography. These devices are not readily available in economically underdeveloped regions of the world, emergency departments, and emergency zones. We have developed a less expensive tool for non-contact monitoring of ACH. The system measures the magnetic induction phase shift (MIPS) between the electromagnetic signals on two coils. ACH was induced in 6 experimental rabbits and edema was induced in 4 control rabbits by stereotactic methods, and their intracranial pressure and heart rate were monitored for 1 h. Signals were continuously monitored for up to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute cerebral hemorrhage; Magnetic induction phase shift; Electromagnetic properties of biological tissues; Intracranial pressure.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200144

A DNA enzyme targeting Egr-1 inhibits rat vascular smooth muscle cell proliferation by down-regulation of cyclin D1 and TGF-β1
Provedor de dados: 56
Autores: Wu,Y.; Han,W.; Liu,G.-N.
We have demonstrated that a synthetic DNA enzyme targeting early growth response factor-1 (Egr-1) can inhibit neointimal hyperplasia following vascular injury. However, the detailed mechanism of this inhibition is not known. Thus, the objective of the present study was to further investigate potential inhibitory mechanisms. Catalytic DNA (ED5) and scrambled control DNA enzyme (ED5SCR) were synthesized and transfected into primary cultures of rat vascular smooth muscle cells (VSMCs). VSMC proliferation and DNA synthesis were analyzed by the MTT method and BrdU staining, respectively. Egr-1, TGF-β1, p53, p21, Bax, and cyclin D1 expression was detected by RT-PCR and Western blot. Apoptosis and cell cycle assays were performed by FACS. Green fluorescence could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA enzyme; Egr-1; VSMC; Proliferation; Apoptosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100004

Effects of sciatic-conditioned medium on neonatal rat retinal cells in vitro
Provedor de dados: 56
Autores: Torres,P.M.M.; Guilarducci,C.V.V.; Araujo,E.G.
Schwann cells produce and release trophic factors that induce the regeneration and survival of neurons following lesions in the peripheral nerves. In the present study we examined the in vitro ability of developing rat retinal cells to respond to factors released from fragments of sciatic nerve. Treatment of neonatal rat retinal cells with sciatic-conditioned medium (SCM) for 48 h induced an increase of 92.5 ± 8.8% (N = 7 for each group) in the amount of total protein. SCM increased cell adhesion, neuronal survival and glial cell proliferation as evaluated by morphological criteria. This effect was completely blocked by 2.5 µM chelerythrine chloride, an inhibitor of protein kinase C (PKC). These data indicate that PKC activation is involved in the effect...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytokines; Neurotrophic factors; Retina; Schwann cells; Sciatic nerve.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100012

Effects of mercury on the arterial blood pressure of anesthetized rats
Provedor de dados: 56
Autores: Rossoni,L.V.; Amaral,S.M.C.; Vassallo,P.F.; França,A.; Oliveira,E.M.; Varner,K.J.; Mill,J.G.; Vassallo,D.V.
The available data suggests that hypotension caused by Hg2+ administration may be produced by a reduction of cardiac contractility or by cholinergic mechanisms. The hemodynamic effects of an intravenous injection of HgCl2 (5 mg/kg) were studied in anesthetized rats (N = 12) by monitoring left and right ventricular (LV and RV) systolic and diastolic pressures for 120 min. After HgCl2 administration the LV systolic pressure decreased only after 40 min (99 ± 3.3 to 85 ± 8.8 mmHg at 80 min). However, RV systolic pressure increased, initially slowly but faster after 30 min (25 ± 1.8 to 42 ± 1.6 mmHg at 80 min). Both right and left diastolic pressures increased after HgCl2 treatment, suggesting the development of diastolic ventricular dysfunction. Since HgCl2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury; Arterial blood pressure; Ventricular pressure; Pulmonary circulation; Diastolic dysfunction; Cholinergic activity.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800009

Usefulness of a rapid immunometric assay for intraoperative parathyroid hormone measurements
Provedor de dados: 56
Autores: Ohe,M.N.; Santos,R.O.; Kunii,I.S.; Carvalho,A.B.; Abrahão,M.; Cervantes,O.; Lazaretti-Castro,M.; Vieira,J.G.H.
Intraoperative parathyroid hormone (IO-PTH) measurements have been proposed to improve operative success rates in primary, secondary and tertiary hyperparathyroidism (PHP, SHP and THP). Thirty-one patients requiring parathyroidectomy were evaluated retrospectively from June 2000 to January 2002. Sixteen had PHP, 7 SHP and 8 THP. Serum samples were taken at times 0 (before resection), 10, 20 and 30 min after resection of each abnormal parathyroid gland. Samples from 28 patients were frozen at -70ºC for subsequent tests, whereas samples from three patients were tested while surgery was being performed. IO-PTH was measured using the Elecsys immunochemiluminometric assay (Roche, Mannheim, Germany). The time necessary to perform the assay was 9 min. All samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parathyroid hormone; Intraoperative PTH measurement; Primary hyperparathyroidism; Secondary hyperparathyroidism; Tertiary hyperparathyroidism.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600006

Malnutrition during lactation as a metabolic imprinting factor inducing the feeding pattern of offspring rats when adults: The role of insulin and leptin
Provedor de dados: 56
Autores: Moura,A.S.; Franco de Sá,C.C.N.; Cruz,H.G.; Costa,C.L.
The aim of the present study was to determine the impact of malnutrition during early postnatal life and the feeding pattern of rat offspring when adults (2 months and 1 year old). In comparison with rats normally fed during lactation, we observed that adult offspring displayed a faster process of feeding reduction when a protein-free diet was offered. In addition, we studied the concentration of insulin and leptin in the lactating pups (10 days) and when these offspring became adult after the onset of a new feeding pattern induced by the protein-free diet. When the diet was changed at 60 days, the offspring malnourished during lactation displayed, after 3 days, a food intake reduction around 41.4 vs 14.2% of the control group. At 10 days of life, plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nutrition; Growth; Insulin; Leptin.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500016

CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
Provedor de dados: 56
Autores: Aguiar,D.P.; Coelho-Aguiar,J.M.; Abreu,J.G.
Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Teratocarcinoma; Adhesion; Condensation; CCN family; CCN2/CTGF; Extracellular matrix.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004

Effect of three exercise programs on patients with chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Dourado,V.Z.; Tanni,S.E.; Antunes,L.C.O.; Paiva,S.A.R.; Campana,A.O.; Renno,A.C.M.; Godoy,I.
We compared the effect of three different exercise programs on patients with chronic obstructive pulmonary disease including strength training at 50_80% of one-repetition maximum (1-RM) (ST; N = 11), low-intensity general training (LGT; N = 13), or combined training groups (CT; N = 11). Body composition, muscle strength, treadmill endurance test (TEnd), 6-min walk test (6MWT), Saint George's Respiratory Questionnaire (SGRQ), and baseline dyspnea (BDI) were assessed prior to and after the training programs (12 weeks). The training modalities showed similar improvements (P &gt; 0.05) in SGRQ-total (ST = 13 ± 14%; CT = 12 ± 14%; LGT = 11 ± 10%), BDI (ST = 1.8 ± 4; CT = 1.8 ± 3; LGT = 1 ± 2), 6MWT (ST = 43 ± 51 m; CT = 48 ± 50 m; LGT = 31 ± 75 m), and TEnd (ST...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic obstructive pulmonary disease; Exercises; Rehabilitation; Strength training.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300007

Nuclear exclusion of transcription factors associated with apoptosis in developing nervous tissue
Provedor de dados: 56
Autores: Linden,R.; Chiarini,L.B.
Programmed cell death in the form of apoptosis involves a network of metabolic events and may be triggered by a variety of stimuli in distinct cells. The nervous system contains several neuron and glial cell types, and developmental events are strongly dependent on selective cell interactions. Retinal explants have been used as a model to investigate apoptosis in nervous tissue. This preparation maintains the structural complexity and cell interactions similar to the retina in situ, and contains cells in all stages of development. We review the finding of nuclear exclusion of several transcription factors during apoptosis in retinal cells. The data reviewed in this paper suggest a link between apoptosis and a failure in the nucleo-cytoplasmic partition of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Programmed cell death; Retina; Neurogenesis; Development; Nuclear membrane.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700003

Hypoxia-induced hyperpermeability of rat glomerular endothelial cells involves HIF-2α mediated changes in the expression of occludin and ZO-1
Provedor de dados: 56
Autores: Luo,Peng-Li; Wang,Yan-Jun; Yang,Yan-Yan; Yang,Jia-Jia.
This study aimed to investigate the role of hypoxia-inducible factor-2α (HIF-2α) in the expression of tight junction proteins and permeability alterations in rat glomerular endothelial cells (rGENCs) under hypoxia conditions. The expression level of HIF-2α and tight junction proteins (occludin and ZO-1) in rGENCs were examined following 5% oxygen density exposure at different treatment times. HIF-2α lentivirus transfection was used to knockdown HIF-2α expression. Cells were divided into four groups: 1) control group (rGENCs were cultured under normal oxygen conditions), 2) hypoxia group (rGENCs were cultured under hypoxic conditions), 3) negative control group (rGENCs were infected with HIF-2α lentivirus negative control vectors and cultured under hypoxic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypoxia; HIF-2α; Rat glomerular endothelial cells; Tight junction; Permeability.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700604

Dietary fiber intake, stool frequency and colonic transit time in chronic functional constipation in children
Provedor de dados: 56
Autores: Guimarães,E.V.; Goulart,E.M.A.; Penna,F.J.
The objective of the present study was to evaluate associations between fiber intake, colonic transit time and stool frequency. Thirty-eight patients aged 4 to 14 years were submitted to alimentary evaluation and to measurement of colonic transit time. The median fiber intake of the total sample was age + 10.3 g/day. Only 18.4% of the subjects presented a daily dietary fiber intake below the levels recommended by the American Health Foundation. In this group, the median left colonic transit time was shorter than in the group with higher dietary fiber intake (11 vs 17 h, P = 0.067). The correlation between stool frequency and colonic transit time was negative and weak for left colon (r = -0.3, P = 0.04), and negative and moderate for rectosigmoid and total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Children; Constipation; Dietary fiber; Colonic transit time.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900007

Immune and hormonal activity in adults suffering from depression
Provedor de dados: 56
Autores: Nunes,S.O.V.; Reiche,E.M.V.; Morimoto,H.K.; Matsuo,T.; Itano,E.N.; Xavier,E.C.D.; Yamashita,C.M.; Vieira,V.R.; Menoli,A.V.; Silva,S.S.; Costa,F.B.; Reiche,F.V.; Silva,F.L.V.; Kaminami,M.S.
An association between depression and altered immune and hormonal systems has been suggested by the results of many studies. In the present study we carried out immune and hormonal measurements in 40 non-medicated, ambulatory adult patients with depression determined by CID-10 criteria and compared with 34 healthy nondepressed subjects. The severity of the condition was determined with the Hamilton Depression Rating Scale. Of 40 depressed patients, 31 had very severe and 9 severe or moderate depression, 29 (72.5%) were females and 11 (27.5%) were males (2.6:1 ratio). The results revealed a significant reduction of albumin and elevation of alpha-1, alpha-2 and ß-globulins, and soluble IL-2 receptor in patients with depression compared to the values obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Depression; Immunity; Psychoneuroimmunology; Cytokines; Acute phase proteins.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500011

Comparison of angular frequency contrast sensitivity in young and older adults
Provedor de dados: 56
Autores: Santos,N.A.; Simas,M.L.B.; Nogueira,R.M.T.B.L.
The aim of the present study was to measure contrast sensitivity curves for angular frequencies in the range between 2 and 96 cycles/360º in older human adult volunteers and to compare these measurements with the more usual contrast sensitivity functions for sine-wave gratings. All subjects were free of identifiable ocular disease and had normal acuity. We measured the contrast thresholds for young adults (N = 6; age range, 20-26 years) and older adults (N = 6; age range, 60-67 years) using the psychophysical forced-choice method. In this paradigm the volunteers had to choose the stimulus containing a test frequency at low contrast (e.g., either a sine-wave grating or an angular frequency stimulus), or another neutral stimulus at mean luminance (without...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Human aging; Spatial frequency; Angular frequency; Contrast sensitivity; Forced-choice method.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300014

Reproducibility of carotid ultrasound measurements in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) at baseline
Provedor de dados: 56
Autores: Santos-Neto,P.J.; Sena-Santos,E.H.; Meireles,D.P.; Santos,I.S.; Bensenor,I.M.; Lotufo,P.A.
Carotid artery assessment by ultrasound is a non-invasive evaluation of subclinical atherosclerosis and a predictor of cardiovascular events. However, ultrasound examinations are operator-dependent. In the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil), ultrasound images have been acquired from more than 10,000 participants. In this article, we describe the reproducibility of carotid intima-media thickness (CIMT), carotid plaque detection, and carotid plaque score (defined as the number of arterial sites with plaques) using ELSA-Brasil protocol, in a subset of 118 participants. Two board-certified radiologists and a trained technician read carotid images. We calculated intra- and inter-observer intraclass correlation (ICC) for CIMT values. We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plaque; Intima-media; Epidemiology; Ultrasound; Imaging.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800610

Exercise improved lipid metabolism and insulin sensitivity in rats fed a high-fat diet by regulating glucose transporter 4 (GLUT4) and musclin expression
Provedor de dados: 56
Autores: Yu,J.; Zheng,J.; Liu,X.F.; Feng,Z.L.; Zhang,X.P.; Cao,L.L.; Zhou,Z.P.
This study aimed to evaluate the effects of exercise training on triglyceride deposition and the expression of musclin and glucose transporter 4 (GLUT4) in a rat model of insulin resistance. Thirty male Sprague-Dawley rats (8 weeks old, weight 160±10 g) were fed a high-fat diet (40% calories from fat) and randomly divided into high-fat control group and swimming intervention group. Rats fed with standard food served as normal control. We found that 8-week swimming intervention significantly decreased body weight (from 516.23±46.27 to 455.43±32.55 g) and visceral fat content (from 39.36±2.50 to 33.02±2.24 g) but increased insulin sensitivity index of the rats fed with a high-fat diet. Moreover, swimming intervention improved serum levels of TG (from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin resistance; Musclin; GLUT4; Swimming; Triglyceride deposition.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500604

Genetic susceptibility to HPV infection and cervical cancer
Provedor de dados: 56
Autores: Maciag,P.C.; Villa,L.L.
Squamous cell carcinoma of the cervix (SCCC) is one of the leading causes of death in developing countries. Infection with high-risk human papillomavirus (HPV) is the major risk factor to develop malignant lesions in the cervix. Polymorphisms of the MHC and p53 genes seem to influence the outcome of HPV infection and progression to SCCC, although controversial data have been reported. MHC are highly polymorphic genes that encode molecules involved in antigen presentation, playing a key role in immune regulation, while p53 is a tumor suppressor gene that regulates cell proliferation. The HPV E6 protein from high-risk types binds p53 and mediates its degradation by the ubiquitin pathway. The role of these polymorphisms in genetic susceptibility to HPV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Cervical cancer; HLA; P53; Polymorphism.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700017

New vaccine strategies against enterotoxigenic Escherichia coli: I: DNA vaccines against the CFA/I fimbrial adhesin
Provedor de dados: 56
Autores: Alves,A.M.B.; Lásaro,M.O.; Almeida,D.F.; Ferreira,L.C.S.
Stimulation of the mammalian immune system by administration of plasmid DNA has been shown to be an important approach for vaccine development against several pathogens. In the present study we investigated the use of DNA vaccines to induce immune responses against an enteric bacterial pathogen, enterotoxigenic Escherichia coli (ETEC). Three plasmid vectors encoding colonization factor antigen I (CFA/I), an ETEC fimbrial adhesin, were constructed. Eukaryotic cells transfected with each of these plasmids expressed the heterologous antigen in different compartments: bound to the cytoplasmic membrane (pRECFA), accumulated in the cytoplasm (pPolyCFA) or secreted to the outside medium (pBLCFA). BALB/c mice were intramuscularly (im) inoculated with purified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA vaccines; ETEC; Diarrheal disease; CFA/I fimbriae.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200011

Pathogenesis of Salmonella-induced enteritis
Provedor de dados: 56
Autores: Santos,R.L.; Tsolis,R.M.; Bäumler,A.J.; Adams,L.G.
Infections with Salmonella serotypes are a major cause of food-borne diseases worldwide. Animal models other than the mouse have been employed for the study of nontyphoidal Salmonella infections because the murine model is not suitable for the study of Salmonella-induced diarrhea. The microbe has developed mechanisms to exploit the host cell machinery to its own purpose. Bacterial proteins delivered directly into the host cell cytosol cause cytoskeletal changes and interfere with host cell signaling pathways, which ultimately enhance disease manifestation. Recently, marked advances have been made in our understanding of the molecular interactions between Salmonella serotypes and their hosts. Here, we discuss the molecular basis of the pathogenesis of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella typhimurium; Enteritis; Diarrhea; Salmonellosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100002

Single-nucleotide polymorphism analysis of GH, GHR, and IGF-1 genes in minipigs
Provedor de dados: 56
Autores: Tian,Y.G.; Yue,M.; Gu,Y.; Gu,W.W.; Wang,Y.J.
Tibetan (TB) and Bama (BM) miniature pigs are two popular pig breeds that are used as experimental animals in China due to their small body size. Here, we analyzed single-nucleotide polymorphisms (SNPs) in gene fragments that are closely related to growth traits [growth hormone (GH), growth hormone receptor (GHR), and insulin-like growth factor (IGF)-1)] in these pig breeds and a large white (LW) control pig breed. On the basis of the analysis of 100 BMs, 108 TBs, and 50 LWs, the polymorphic distribution levels of GH, GHR, and IGF-1 were significantly different among these three pig breeds. According to correlation analyses between SNPs and five growth traits - body weight (BW), body length (BL), withers height (WH), chest circumference (CC), and abdomen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Minipigs; SNP; GH; GHR; IGF-1.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900753

Interleukin-12 and protective immunity to schistosomes
Provedor de dados: 56
Autores: Mountford,A.P.; Shires,V.L.; Anderson,S.
The attenuated vaccine against Schistosoma mansoni induces Th1-mediated protective immunity and we have sought to identify a role for IL-12 in this model. Elevated levels of IL-12 (p40 mRNA) were detected in the lymph nodes (LN) and the lungs of vaccinated mice, whilst treatment of vaccinated mice with anti-IL-12 antibodies decreased the ratio of IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;:IL-4 secreted by in vitro-cultured LN cells. However, there was only marginal abrogation of the level of resistance in these mice. Soluble antigens from the lung-stage of the parasite (SLAP) appeared to be efficient stimulators of IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; and IL-12 secretion. These antigens when used to immunise mice in conjunction with IL-12 as an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosoma; Interleukin-12; Adjuvant; Vaccine; Th1.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100023

Ilex paraguariensis extracts extend the lifespan of Drosophila melanogaster fed a high-fat diet
Provedor de dados: 56
Autores: Colpo,A.C.; Lima,M.E.; da Rosa,H.S.; Leal,A.P.; Colares,C.C.; Zago,A.C.; Salgueiro,A.C.F.; Bertelli,P.R.; Minetto,L.; Moura,S.; Mendez,A.S.L.; Folmer,V.
Studies have suggested that total energy intake and diet composition affect lifespan and ageing. A high-fat diet induces oxidative stress and affects the development of diseases. In contrast, antioxidants are capable of reducing its harmful effects. Yerba mate beverages are an important source of antioxidants, but there is scarce knowledge about their effects on suppressing fat accumulation. Here, we investigated the compounds present in yerba mate extracts and assessed their effects on Drosophila melanogaster given a high cholesterol diet. LS-ESI-MS analysis showed the presence of matesaponins, phenolic compounds and methylxanthines in all of the examined extracts. In Drosophila, under extract treatment conditions, the mean lifespan was significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yerba mate; Matesaponin; LC-MS; Antioxidants compounds; Chronic diseases; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200601

Alterations in proteins of bone marrow extracellular matrix in undernourished mice
Provedor de dados: 56
Autores: Vituri,C.L.; Alvarez-Silva,M.; Trentin,A.G.; Borelli,P.
The objective of the present study was to determine the effect of protein malnutrition on the glycoprotein content of bone marrow extracellular matrix (ECM). Two-month-old male Swiss mice were submitted to protein malnutrition with a low-protein diet containing 4% casein as compared to 20% casein in the control diet. When the experimental group had attained a 20% loss of their original body weight, we extracted the ECM proteins from bone marrow with PBS buffer, and analyzed ECM samples by SDS-PAGE (7.5%) and ECL Western blotting. Quantitative differences were observed between control and experimental groups. Bone marrow ECM from undernourished mice had greater amounts of extractable fibronectin (1.6-fold increase) and laminin (4.8-fold increase) when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibronectin; Bone marrow; Extracellular matrix; Malnutrition.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800004

Receptor localization of steroid hormones and drugs: discoveries through the use of thaw-mount and dry-mount autoradiography
Provedor de dados: 56
Autores: Stumpf,W.E.
The history of receptor autoradiography, its development and applications, testify to the utility of this histochemical technique for localizing radiolabeled hormones and drugs at cellular and subcellular sites of action in intact tissues. Localization of diffusible compounds has been a challenge that was met through the introduction of the "thaw-mount" and "dry-mount" autoradiographic techniques thirty years ago. With this cellular receptor autoradiography, used alone or combined with other histochemical techniques, sites of specific binding and deposition in vivo and in vitro have been characterized. Numerous discoveries, some reviewed in this article, provided information that led to new concepts and opened new areas of research. As an example, in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitamin D; Steroid hormones; Receptors; Autoradiography; Drug distribution; Localization.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200003

Dyspnea perception in cystic fibrosis patients
Provedor de dados: 56
Autores: Ziegler,B.; Fernandes,A.K.; Sanches,P.R.S.; Junior,D.P. Silva; Thomé,P.R.O.; Dalcin,P.T.R.
We evaluated dyspnea perception in cystic fibrosis patients compared with normal subjects, during an inspiratory resistive loading test and 6-min walk test. We also evaluated the correlation between dyspnea scores induced by resistive loads and by the 6-min walk test. In this prospective, cross-sectional study, 31 patients with cystic fibrosis (≥15 years of age) and 31 age-, gender-, and ethnicity-matched healthy volunteers (20 females and 11 males per group) underwent inspiratory resistive loading, spirometry, and the 6-min walk test. As the magnitude of the inspiratory loads increased, dyspnea scores increased (P&lt;0.001), but there was no difference between groups in dyspnea score (P=0.654). Twenty-six (84%) normal subjects completed all the resistive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cystic fibrosis; Dyspnea perception; Pulmonary function test; Six-min walk test; Inspiratory resistive load testing.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000897

miRNA expression profiles in chronic lymphocytic and acute lymphocytic leukemia
Provedor de dados: 56
Autores: Zanette,D.L.; Rivadavia,F.; Molfetta,G.A.; Barbuzano,F.G.; Proto-Siqueira,R.; Falcão,R.P.; Zago,M.A.; Silva-Jr,W.A.
MicroRNAs (miRNAs) are a class of small endogenous RNAs that play important regulatory roles by targeting mRNAs for cleavage or translational repression. miRNAs act in diverse biological processes including development, cell growth, apoptosis, and hematopoiesis, suggesting their association with cancer. We determined the miRNA expression profile of chronic and acute lymphocytic leukemias (CLL and ALL) using the TaqMan® MicroRNA Assays Human Panel (Applied Biosystems). Pooled leukemia samples were compared to pooled CD19+ samples from healthy individuals (calibrator) by the 2-DDCt method. Total RNA input was normalized based on the Ct values obtained for hsa-miR-30b. The five most highly expressed miRNAs were miR-128b, miR-204, miR-218, miR-331, and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Non-coding RNA; MicroRNA; Hematopoietic malignancies; Leukemia; MiR-331.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100003

Intensity of swimming exercise influences aortic reactivity in rats
Provedor de dados: 56
Autores: Brito,A.F.; Silva,A.S.; Souza,I.L.L.; Pereira,J.C.; Silva,B.A. da.
Exercise is known to cause a vasodilatory response; however, the correlation between the vasorelaxant response and different training intensities has not been investigated. Therefore, this study evaluated the vascular reactivity and lipid peroxidation after different intensities of swimming exercise in rats. Male Wistar rats (aged 8 weeks; 250-300 g) underwent forced swimming for 1 h whilst tied to loads of 3, 4, 5, 6, and 8% of their body weight, respectively (groups G3, G4, G5, G6 and G8, respectively; n=5 each). Immediately after the test, the aorta was removed and suspended in an organ bath. Cumulative relaxation in response to acetylcholine (10−12-10−4 M) and contraction in response to phenylephrine (10−12-10−5 M) were measured. Oxidative stress was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aerobic exercise; Anaerobic exercise; Lipid peroxidation; Smooth muscle; Swimming; Vessel.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001100996

Th1 polarized response induced by intramuscular DNA-HSP65 immunization is preserved in experimental atherosclerosis
Provedor de dados: 56
Autores: Fonseca,D.M.; Bonato,V.L.D.; Silva,C.L.; Sartori,A.
We previously reported that a DNA vaccine constructed with the heat shock protein (HSP65) gene from Mycobacterium leprae (DNA-HSP65) was protective and also therapeutic in experimental tuberculosis. By the intramuscular route, this vaccine elicited a predominant Th1 response that was consistent with its protective efficacy against tuberculosis. It has been suggested that the immune response to Hsp60/65 may be the link between exposure to microorganisms and increased cardiovascular risk. Additionally, the high cholesterol levels found in atherosclerosis could modulate host immunity. In this context, we evaluated if an atherogenic diet could modulate the immune response induced by the DNA-HSP65 vaccine. C57BL/6 mice (4-6 animals per group) were initially...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA-HSP65; Genetic vaccine; Atherosclerosis; Tuberculosis; Th1; Cholesterol.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100010

Radial frequency stimuli and sine-wave gratings seem to be processed by distinct contrast brain mechanisms
Provedor de dados: 56
Autores: Simas,M.L.B.; Nogueira,R.M.T.B.L.; Santos,N.A.
An assumption commonly made in the study of visual perception is that the lower the contrast threshold for a given stimulus, the more sensitive and selective will be the mechanism that processes it. On the basis of this consideration, we investigated contrast thresholds for two classes of stimuli: sine-wave gratings and radial frequency stimuli (i.e., j0 targets or stimuli modulated by spherical Bessel functions). Employing a suprathreshold summation method, we measured the selectivity of spatial and radial frequency filters using either sine-wave gratings or j0 target contrast profiles at either 1 or 4 cycles per degree of visual angle (cpd), as the test frequencies. Thus, in a forced-choice trial, observers chose between a background spatial (or radial)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sine-wave gratings; Spatial frequency; Radial frequency; Bessel functions; Multiple channels; Contrast sensitivity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300013

Exaggerated blood pressure response during the exercise treadmill test as a risk factor for hypertension
Provedor de dados: 56
Autores: Lima,S.G.; Albuquerque,M.F.P.M.; Oliveira,J.R.M.; Ayres,C.F.J.; Cunha,J.E.G.; Oliveira,D.F.; Lemos,R.R.; Souza,M.B.R.; Silva,O. Barbosa e.
Exaggerated blood pressure response (EBPR) during the exercise treadmill test (ETT) has been considered to be a risk factor for hypertension. The relationship of polymorphisms of the renin-angiotensin system gene with hypertension has not been established. Our objective was to evaluate whether EBPR during exercise is a clinical marker for hypertension. The study concerned a historical cohort of normotensive individuals. The exposed individuals were those who presented EBPR. At the end of the observation period (41.7 months = 3.5 years), the development of hypertension was analyzed within the two groups. Genetic polymorphisms and blood pressure behavior were assessed as independent variables, together with the classical risk factors for hypertension. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Risk factors; Exaggerated blood pressure response.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000400368

RNAi-mediated knockdown of pituitary tumor- transforming gene-1 (PTTG1) suppresses the proliferation and invasive potential of PC3 human prostate cancer cells
Provedor de dados: 56
Autores: Huang,S.Q.; Liao,Q.J.; Wang,X.W.; Xin,D.Q.; Chen,S.X.; Wu,Q.J.; Ye,G.
Pituitary tumor-transforming gene-1 (PTTG1) is a proto-oncogene that promotes tumorigenesis and metastasis in numerous cell types and is overexpressed in a variety of human tumors. We have demonstrated that PTTG1 expression was up-regulated in both human prostate cancer specimens and prostate cancer cell lines. For a more direct assessment of the function of PTTG1 in prostate tumorigenesis, RNAi-mediated knockdown was used to selectively decrease PTTG1 expression in PC3 human prostate tumor cells. After three weeks of selection, colonies stably transfected with PTTG1-targeted RNAi (the knockdown PC3 cell line) or empty vector (the control PC3 cell line) were selected and expanded to investigate the role of PTTG1 expression in PC3 cell growth and invasion....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PTTG1; Prostate cancer; Cell proliferation; Transwell invasion assay; Anticancer drug resistance.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100001

Dietary sodium intake induced myenteric neuron hypertrophy in Wistar rats
Provedor de dados: 56
Autores: De Souza,R.R.; Gama,E.F.; Silva,R.D.A.; Heimann,J.C.; Maifrino,L.B.M.; Liberti,E.A.
In the present study we investigated the effect of salt intake on myenteric neuron size of the colon of adult male Wistar rats. The animals were placed on either a high-salt (HS; 8%; 12 animals) or a low-salt diet (LS; 0.15%; 12 animals) for 15 or 52 weeks and blood pressure was measured. The sizes of myenteric neurons of the distal colon from both groups were measured. No difference in neuron size was observed between the HS and LS groups after 15 weeks. After 52 weeks on HS, neuron size was increased (P&amp;lt;0.005) when compared with the LS group. The rats also presented hypertension, which was significantly different at 52 weeks (142 ± 11 vs 119 ± 7 mmHg). These results suggest that a long time on an HS diet can significantly increase myenteric nerve...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myenteric plexus; Neuron size; Cell growth; Sodium intake.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700017

Paradoxical sleep deprivation increases plasma endothelin levels
Provedor de dados: 56
Autores: Palma,B.D.; Gabriel Jr.,A.; Bignotto,M.; Tufik,S.
The endothelins (ET-1, 2 and 3) constitute a family of 21 amino acid peptides with potent biological activities. ET-1 is one of the most potent endogenous vasoconstrictors so far identified and its increased concentration in plasma appears to be closely related to the pathogenesis of arterial hypertension as well as to obstructive sleep apnea (OSA). OSA patients exhibit repetitive episodes of apnea and hypopnea that result in hypoxia and consecutive arousals. These patients are chronically sleep deprived, which may aggravate the hypertensive features, since literature data show that sleep deprivation results in hypertension both in humans and in animals. Based on the reported relationship between ET-1, hypertension and sleep deprivation consequences, the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Endothelin; Sleep deprivation; Rat; Hypertension.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100011

Association of angiotensin-converting enzyme activity and polymorphism with echocardiographic measures in familial and nonfamilial hypertrophic cardiomyopathy
Provedor de dados: 56
Autores: Buck,P.C.; Fernandes,F.; Arteaga,E.; Matsumoto,A.Y.; Araujo,A.Q.; Oliveira,E.M.; Ianni,B.M.; Ramires,F.J.A.; Krieger,J.E.; Mady,C.
Angiotensin-converting enzyme (ACE) activity and polymorphism contribute significantly to the prognosis of patients with cardiomyopathy. The aim of this study was to determine the activity and type of ACE polymorphism in patients with familial and nonfamilial hypertrophic cardiomyopathy (HCM) and to correlate these with echocardiographic measurements (echo-Doppler). We studied 136 patients (76 males) with HCM (69 familial and 67 nonfamilial cases). Mean age was 41 ± 17 years. DNA was extracted from blood samples for the polymerase chain reaction and the determination of plasma ACE levels. Left ventricular mass, interventricular septum, and wall thickness were measured. Mean left ventricular mass index, interventricular septum and wall thickness in familial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Echocardiography; ACE activity; ACE polymorphism; Hypertrophic cardiomyopathy; Left ventricular mass index.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800005

Liver oxidative stress of the grey mullet Mugil cephalus presents seasonal variations in Ennore estuary
Provedor de dados: 56
Autores: Padmini,E.; Vijaya Geetha,B.; Usha Rani,M.
The objective of this study was to determine the liver oxidative stress status of grey mullets living in heavy-metal-rich polluted Ennore estuary compared with unpolluted Kovalam estuary. Fish were collected from both estuaries during the monsoon and summer seasons from October 2004 to September 2006. Fish liver homogenate (N = 20 per group) was prepared for evaluating oxidative stress parameters. Fish living in the polluted estuary had significantly higher lipid oxidation products, conjugated dienes (0.346 ± 0.017 vs 0.141 ± 0.012 DA233/mg protein), lipid hydroperoxides (0.752 ± 0.032 vs 0.443 ± 0.03 nmol/mg protein), and lipid peroxides (3.447 ± 0.14vs 1.456 ± 0.096 nmol MDA/mg protein) than those of the unpolluted estuary during the summer. In contrast,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Antioxidant defense; Estuary; Grey mullets; Oxidative stress; Pollutants.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100002

Cancer research in Brazil - stuck in second gear?
Provedor de dados: 56
Autores: Lepique,A.P.; Bonorino,C.
This article describes the main issues regarding clinical cancer research in Brazil, including both the opportunities and the hurdles. Scientists and clinicians in this field had the opportunity to talk to regulatory agencies and to the Health Ministry representative at a meeting held in the State of Rio de Janeiro, Brazil, in April 2014. Our conclusions are that we do indeed have opportunities; however, we need to move forward regarding partnerships between academia and industry, increase the availability of funding, and provide easier navigation through the regulatory processes.
Palavras-chave: Cancer research; Clinical research; Regulatory agencies; Health Ministry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200108

Necroptosis occurs in osteoblasts during tumor necrosis factor-α stimulation and caspase-8 inhibition
Provedor de dados: 56
Autores: Shi,Guan; Jia,Pu; Chen,Hao; Bao,Li; Feng,Fei; Tang,Hai.
Necroptosis is a regulated cell death mechanism. However, it is unknown whether necroptosis is involved in the death of tumor necrosis factor-α (TNF-α)-treated osteoblasts. Therefore, we conducted the study with TNF-α, Nec-1 (a specific inhibitor of necroptosis), and Z-IETD-FMK (a specific inhibitor of apoptosis) to determine whether necroptosis plays a role in the death of TNF-α-treated osteoblast cell line MC3T3-E1. Cell viability, cell death, and lactate dehydrogenase (LDH) release were assayed to evaluate cytotoxicity. Specific marker proteins receptor interacting protein kinase (RIPK3) and phosphorylated mixed lineage kinase domain-like protein (p-MLKL) for necroptosis, and cleaved caspase 3 for apoptosis were detected by western blot, and mRNA was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Necroptosis; Mouse osteoblast; Z-IETD-FMK; Necrostatin-1; TNF-α.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100603

lncRNA OIP5-AS1 targets ROCK1 to promote cell proliferation and inhibit cell apoptosis through a mechanism involving miR-143-3p in cervical cancer
Provedor de dados: 56
Autores: Song,Linlin; Wang,Linlin; Pan,Xiaoli; Yang,Caihong.
Opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) is one kind of cytoplasmic long non-coding RNA (lncRNA), which has been demonstrated to play a critical function in multiple cancers. However, the detailed mechanism of OIP5-AS1 in the regulation of cervical cancer progression is still obscure. Here, we demonstrated that lncRNA OIP5-AS1 was upregulated in cervical cancer and was correlated with poor prognosis by bioinformatics studies. OIP5-AS1 depletion inhibited cell proliferation and promoted cell apoptosis in cervical cancer cells. Furthermore, we clarified that ROCK1 was the downstream effector of OIP5-AS1 and OIP5-AS1 acted as a molecular sponge of miR-143-3p. Finally, we verified that OIP5-AS1 exerted its function in the regulation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: LncRNA; OIP5-AS1; Cervical cancer; MiR-143-3p; ROCK1.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100608

Inadequate utilization of prenatal care in two Brazilian birth cohorts
Provedor de dados: 56
Autores: Coimbra,L.C.; Figueiredo,F.P.; Silva,A.A.M.; Barbieri,M.A.; Bettiol,H.; Caldas,A.J.M.; Mochel,E.G.; Ribeiro,V.S.
Data for two birth cohorts from two Brazilian municipalities, Ribeirão Preto in 1994 and São Luís in 1997/1998, were used to identify and compare factors associated with inadequate utilization of prenatal care and to identify factors capable of explaining the differences observed between the two cities. Prenatal care was defined as adequate or inadequate according to the recommendations of the Brazilian Ministry of Health. The chi-square test and Poisson regression were used to compare differences in the inadequacy of prenatal care utilization. The percentage of inadequacy was higher in São Luís (34.6%) than in Ribeirão Preto (16.9%). Practically the same variables were associated with inadequacy in both cities. Puerperae with lower educational level,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prenatal care; Maternal health; Quality of health services.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000900006

Angiotensin-(1-7) increases osmotic water permeability in isolated toad skin
Provedor de dados: 56
Autores: Santos,J.C.; Jerez,S.; Peral de Bruno,M.; Coviello,A.
Angiotensin-(1-7) (Ang-(1-7)) increased osmotic water permeability in the isolated toad skin, a tissue with functional properties similar to those of the distal mammalian nephron. Concentrations of 0.1 to 10 µM were effective, with a peak at 20 min. This effect was similar in magnitude to that of frog skin angiotensin II (Ang II) and oxytocin but lower than that of human Ang II and arginine-vasotocin. The AT2 angiotensin receptor antagonist PD 123319 (1.0 µM) fully inhibited the response to 0.1 µM Ang-(1-7) but had no effect on the response to Ang II at the same concentration. The specific receptor antagonist of Ang-(1-7), A-779, was ineffective in blocking the response to Ang-(1-7) and to frog skin Ang II. The AT1 receptor subtype antagonist losartan,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin-(1-7); Toad skin; Osmotic water permeability; Receptor subtypes.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900018

Skin morphology of the mutant hairless USP mouse
Provedor de dados: 56
Autores: Massironi,S.M.G.; Giacóia,M.R.; Maiorka,P.C.; Kipnis,T.L.; Dagli,M.L.Z.
The morphology of the skin of the mutant hairless USP mouse was studied by histological, histochemical and immunohistochemical methods and compared to the skin of BALB/c mice. Representative sections of the dorsal skin from mice of both strains aged 18 days, and 1, 3, 6, and 8 months were studied. Sections stained with hematoxylin and eosin showed cystic formations called utricles and dermal cysts in the dermis that increased in size and number during growth. Skin thickness increased significantly at 8 months. Sections stained with picrosirius and examined with polarized light, displayed different colors, suggesting different thicknesses of dermal collagen fibers (probably types I and III). Weigert, Verhoeff and resorcin-fuchsin stains revealed fibers of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutant mice; HrUSP mutants; Hairless mice; BALB/c mice; Ethyl-nitrosourea.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100006

Therapeutic effects of Sophora moorcroftiana alkaloids in combination with albendazole in mice experimentally infected with protoscolices of Echinococcus granulosus
Provedor de dados: 56
Autores: Ma,X.M.; Bao,G.SH.; Wan,J.M.; Liao,D.J.; Yin,SH.F.; Meng,X.Q.; Zhou,G.K.; Lu,X.M.; Li,H.Y.
The objective of the present study was to determine if the combination of alkaloids from Sophora moorcroftiana seeds and albendazole might be effective in the treatment of experimental echinococcosisin female NIH mice (6 weeks old and weighing 18-20 g, N = 8 in each group) infected withprotoscolices of Echinococcus granulosus. Viable protoscolices (N = 6 x 103) were cultured in vitro in 1640 medium and mortality was calculated daily. To determine the in vivo efficacy, mice were inoculated intraperitoneally with viable protoscolices and then treated once daily by gavage for three months with the alkaloids (50 mg kg-1 day-1) and albendazole (50 mg kg-1 day-1), separately and in combination (both alkaloids at 25 mg kg-1 day-1 and albendazole at 25 mg kg-1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sophora moorcroftiana; Echinococcus granulosus; Alkaloids; Cytokines; Ethnobotany; Therapeutics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000014

Histologic distribution of insulin and glucagon receptors
Provedor de dados: 56
Autores: Watanabe,M.; Hayasaki,H.; Tamayama,T.; Shimada,M.
Insulin and glucagon are the hormonal polypeptides secreted by the B and A cells of the endocrine pancreas, respectively. Their major physiologic effects are regulation of carbohydrate metabolism, but they have opposite effects. Insulin and glucagon have various physiologic roles, in addition to the regulation of carbohydrate metabolism. The physiologic effects of insulin and glucagon on the cell are initiated by the binding of each hormone to receptors on the target cells. Morphologic studies may be useful for relating biochemical, physiologic, and pharmacologic information on the receptors to an anatomic background. Receptor radioautography techniques using radioligands to label specific insulin and glucagon receptors have been successfully applied to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radioautography; Insulin; Glucagon; Receptor; Distribution.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200008

Molluscicidal activity of Punica granatum bark and Canna indica root
Provedor de dados: 56
Autores: Tripathi,S.M.; Singh,D.K.
The molluscicidal activity of Punica granatum Linn. (Punicaceae) and Canna indica Linn. (Cannaceae) against the snail Lymnaea acuminata was studied. The molluscicidal activity of P. granatum bark and C. indica root was found to be both time and dose dependent. The toxicity of P. granatum bark was more pronounced than that of C. indica. The 24 h LC50 of the column-purified root of C. indica was 6.54 mg/l whereas that of the column-purified bark of P. granatum was 4.39 mg/l. The ethanol extract of P. granatum (24 h LC50: 22.42 mg/l) was more effective than the ethanol extract of C. indica (24 h LC50: 55.65 mg/l) in killing the test animals. P. granatum and C. indica may be used as potent molluscicides since the concentrations used to kill the snails were not...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Punica granatum; Canna indica; Plant molluscicide; Lymnaea acuminata.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100014

Association of HDL cholesterol and triglycerides with mortality in patients with heart failure
Provedor de dados: 56
Autores: Freitas,H.F.G.; Barbosa,E.A.; Rosa,F.H.F.P.; Lima,A.C.P.; Mansur,A.J.
It has been demonstrated that there is an association between serum lipoproteins and survival rate in patients with ischemic cardiomyopathy, as well as in patients with non-ischemic causes of heart failure. We tested the hypothesis of an association between serum lipoprotein levels and prognosis in a cohort of outpatients with heart failure, including Chagas' heart disease. The lipid profile of 833 outpatients with heart failure in functional classes III and IV of the New York Heart Association, with a mean age of 46.9 ± 10.6 years, 655 (78.6%) men and 178 (21.4%) women, was studied from April 1991 to June 2003. The survival rate was estimated by the Kaplan-Meyer's method and the Cox proportional hazards models. Etiology of heart failure was ischemic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Prognosis; HDL cholesterol; Chagas' heart disease; Triglycerides.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500004

High mobility group box 1 as a mediator of endotoxin administration after hemorrhagic shock-primed lung injury
Provedor de dados: 56
Autores: Guo,F.; Shi,Y.; Xu,H.; Ding,J.
High mobility group box 1 (HMGB1) was discovered as a novel late-acting cytokine that contributes to acute lung injury (ALI). However, the contribution of HMGB1 to two-hit-induced ALI has not been investigated. To examine the participation of HMGB1 in the pathogenesis of ALI caused by the two-hit hypothesis, endotoxin was injected intratracheally in a hemorrhagic shock-primed ALI mouse model. Concentrations of HMGB1 in the lung of the shock group were markedly increased at 16 h (1.63 ± 0.05, compared to the control group: 1.02 ± 0.03; P &lt; 0.05), with the highest concentration being observed at 24 h. In the sham/lipopolysaccharide group, lung HMGB1 concentrations were found to be markedly increased at 24 h (1.98 ± 0.08, compared to the control group:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute lung injury; High mobility group box 1; Two-hit-induced ALI; Hemorrhagic shock; Endotoxin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900006

Kaposi's sarcoma-associated herpesvirus infection and Kaposi's sarcoma in Brazil
Provedor de dados: 56
Autores: Ramos-da-Silva,S.; Elgui-de-Oliveira,D.; Borges,L.; Bacchi,C.E.
Kaposi's sarcoma (KS) became a critical health issue with the emergence of acquired immunodeficiency syndrome (AIDS) in the 1980s. Four clinical-epidemiological forms of KS have been described: classical KS, endemic KS, iatrogenic KS, and AIDS-associated KS. In 1994, Kaposi's sarcoma-associated herpesvirus (KSHV) or human herpesvirus type 8 was identified by Chang and colleagues, and has been detected worldwide at frequencies ranging from 80 to 100%. The aim of the present study was to evaluate the frequency of KSHV infection in KS lesions from HIV-positive and HIV-negative patients in Brazil, as well as to review the current knowledge about KS transmission and detection. For these purposes, DNA from 51 cases of KS was assessed by PCR: 20 (39.2%) cases of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kaposi's sarcoma; Human herpesvirus type 8; Kaposi's sarcoma-associated herpesvirus; Polymerase chain reaction; Viral carcinogenesis; Brazil.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500002

Properties of a constitutive alkaline phosphatase from strain 74A of the mold Neurospora crassa
Provedor de dados: 56
Autores: Morales,A.C.; Nozawa,S.R.; Thedei Jr.,G.; Maccheroni Jr.,W.; Rossi,A.
A constitutive alkaline phosphatase was purified to apparent homogeneity as determined by polyacrylamide gel electrophoresis from mycelia of the wild strain 74A of the mold Neurospora crassa, after growth on acetate and in the presence of saturating amounts of inorganic phosphate (Pi) for 72 h at 30ºC. The molecular mass was 58 kDa and 56 kDa as determined by exclusion chromatography and SDS-PAGE, respectively. This monomeric enzyme shows an apparent optimum pH ranging from 9.5 to 10.5 and Michaelis kinetics for the hydrolysis of p-nitrophenyl phosphate (the Km and Hill coefficient values were 0.35 mM and 1.01, respectively), alpha-naphthyl phosphate (the Km and Hill coefficient values were 0.44 mM and 0.97, respectively), ß-glycerol phosphate (the Km and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurospora crassa; Fungi; Alkaline phosphatase; L-histidinol-Pi phosphatase.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800006

Brazilian Journal of Medical and Biological Research 1981-2002
Provedor de dados: 56
Autores: Krieger,Eduardo Moacyr; Ferreira,Sérgio Henrique.
Tipo: Info:eu-repo/semantics/other
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100001

MicroRNA profiling identifies miR-7-5p and miR-26b-5p as differentially expressed in hypertensive patients with left ventricular hypertrophy
Provedor de dados: 56
Autores: Kaneto,C.M.; Nascimento,J.S.; Moreira,M.C.R.; Ludovico,N.D.; Santana,A.P.; Silva,R.A.A.; Silva-Jardim,I.; Santos,J.L.; Sousa,S.M.B.; Lima,P.S.P.
Recent evidence suggests that cell-derived circulating miRNAs may serve as biomarkers of cardiovascular diseases. However, a few studies have investigated the potential of circulating miRNAs as biomarkers for left ventricular hypertrophy (LVH). In this study, we aimed to characterize the miRNA profiles that could distinguish hypertensive patients with LHV, hypertensive patients without LVH and control subjects, and identify potential miRNAs as biomarkers of LVH. LVH was defined by left ventricular mass indexed to body surface area &gt;125 g/m2 in men and &gt;110 g/m2 in women and patients were classified as hypertensive when presenting a systolic blood pressure of 140 mmHg or more, or a diastolic blood pressure of 90 mmHg or more. We employed miRNA PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular disease; Hypertension; Left ventricular hypertrophy; MicroRNAs.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200605

Raising awareness of chronic kidney disease in a Brazilian urban population
Provedor de dados: 56
Autores: Mazza Nascimento,M.; Riella,M.C.
The incidence and prevalence of chronic kidney disease have been increasing in recent years in developing countries. The aim of this study was to report the results of a general chronic kidney disease awareness program applied to an urban population in a large Brazilian city. From January 2002 to January 2005 a total of 8883 individuals in the city of Curitiba (PR, Brazil) were screened for hypertension, body mass index, hematuria, and proteinuria. A family history and previous medical diagnosis of hypertension and diabetes mellitus (DM) were also recorded. Of the 8883 individuals assessed, 56% were women, subject median age was 47 years (range: 17-93 years) and more than 90% were Caucasian. Thirty percent had signs of hematuria, 6% had proteinuria, and 3%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematuria; Proteinuria; Prevention; Chronic kidney disease; Screening.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800010

Reliability and validity of the interactive observation scale for psychiatric inpatients applied by nursing aides in daily ward practice
Provedor de dados: 56
Autores: Pedrão,L.J.; Hallak,J.E.C.; Bernardo,S.A.; Zuardi,A.W.
The main characteristic of the nursing Interactive Observation Scale for Psychiatric Inpatients (IOSPI) is the necessity of interaction between raters and patients during assessment. The aim of this study was to evaluate the reliability and validity of the scale in the "real" world of daily ward practice and to determine whether the IOSPI can increase the interaction time between raters and patients and influence the raters' opinion about mental illness. All inpatients of a general university hospital psychiatric ward were assessed daily over a period of two months by 9 nursing aides during the morning and afternoon shifts, with 273 pairs of daily observations. Once a week the patients were interviewed by a psychiatrist who filled in the Brief Psychiatric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nurse's rating scale; Reliability; Validity; Psychiatric inpatient.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700013

Enhancement of cellular activity in hyperglycemic mice dermal wounds dressed with chitosan-alginate membranes
Provedor de dados: 56
Autores: Breder,J.S.C.; Pires,A.L.R.; Azevedo,F.F.; Apolinário,P.P.; Cantaruti,T.; Jiwani,S.I.; Moraes,Â.M.; Consonni,S.R.; Araújo,E.P.; Adams,G.G.; Saad,M.J.A.; Lima,M.H.M.
The use of specially designed wound dressings could be an important alternative to facilitate the healing process of wounds in the hyperglycemic state. Biocompatible dressings combining chitosan and alginate can speed up wound healing by modulating the inflammatory phase, stimulating fibroblast proliferation, and aiding in remodeling phases. However, this biomaterial has not yet been explored in chronic and acute lesions of diabetic patients. The aim of this study was to evaluate the effect of topical treatment with a chitosan-alginate membrane on acute skin wounds of hyperglycemic mice. Diabetes mellitus was induced by streptozotocin (60 mg · kg-1 · day-1 for 5 days, intraperitoneally) and the cutaneous wound was performed by removing the epidermis using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C57BL/6J mice; Wound healing; Wound dressing; Polysaccharides; Diabetes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100602

Pamidronate for the treatment of osteoporosis secondary to chronic cholestatic liver disease in Wistar rats
Provedor de dados: 56
Autores: Pereira,F.A.; Mattar,R.; Facincani,I.; Defino,H.L.A.; Ramalho,L.N.Z.; Jorgetti,V.; Volpon,J.B.; Paula,F.J.A. de.
Osteoporosis is a major complication of chronic cholestatic liver disease (CCLD). We evaluated the efficacy of using disodium pamidronate (1.0 mg/kg body weight) for the prevention (Pr) or treatment (Tr) of cholestasis-induced osteoporosis in male Wistar rats: sham-operated (Sham = 12); bile duct-ligated (Bi = 15); bile duct-ligated animals previously treated with pamidronate before and 1 month after surgery (Pr = 9); bile duct-ligated animals treated with pamidronate 1 month after surgery (Tr = 9). Rats were sacrificed 8 weeks after surgery. Immunohistochemical expression of IGF-I and GH receptor was determined in the proximal growth plate cartilage of the left tibia. Histomorphometric analysis was performed in the right tibia and the right femur was used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic osteodystrophy; Osteoporosis pathogenesis; Growth factors; Biomechanics; Animal models; Pamidronate.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200021

Evasion of immune responses by Trypanosoma cruzi, the etiological agent of Chagas disease
Provedor de dados: 56
Autores: DosReis,G.A.
Infection with the protozoan parasite Trypanosoma cruzi leads to Chagas disease, which affects millions of people in Latin America. Infection with T. cruzi cannot be eliminated by the immune system. A better understanding of immune evasion mechanisms is required in order to develop more effective vaccines. During the acute phase, parasites replicate extensively and release immunomodulatory molecules that delay parasite-specific responses mediated by T cells. This immune evasion allows the parasite to spread in the host. In the chronic phase, parasite evasion relies on its replication strategy of hijacking the TGF-β signaling pathway involved in inflammation and tissue regeneration. In this article, the mechanisms of immune evasion described for T. cruzi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; T cells; GPI-anchors; TGF-β; Mucin; Apoptosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200001

Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection
Provedor de dados: 56
Autores: Shulzhenko,N.; Morgun,A.; Franco,M.; Souza,M.M.; Almeida,D.R.; Diniz,R.V.Z.; Carvalho,A.C.C.; Pacheco-Silva,A.; Gerbase-DeLima,M.
The purpose of the present study was to investigate the expression (mRNA) of CD40 ligand (CD40L), interferon-gamma (IFN-gamma) and Fas ligand (FasL) genes in human cardiac allografts in relation to the occurrence of acute cardiac allograft rejection as well as its possible value in predicting acute rejection. The mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction method in 39 samples of endomyocardial biopsies obtained from 10 adult cardiac transplant recipients within the first six months after transplantation. Biopsies with ongoing acute rejection showed significantly higher CD40L, IFN-gamma and FasL mRNA expression than biopsies without rejection. The median values of mRNA expression in biopsies with and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rejection; Cardiac allograft; Immunologic monitoring; MRNA; Gene activation; Interferon-gamma.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600013

Association of ApoE polymorphisms with prevalent hypertension in 1406 older adults: the Bambuí Health Aging Study (BHAS)
Provedor de dados: 56
Autores: Fuzikawa,A.K.; Peixoto,S.V.; Taufer,M.; Moriguchi,E.H.; Lima-Costa,M.F.
Apolipoprotein E (ApoE) polymorphism influences lipid metabolism, but its association with arterial hypertension is controversial. The objective of this study was to examine the association between ApoE polymorphism and prevalent hypertension in a large unselected population of older adults. Participants from the baseline of the Bambuí Health Aging Study whose ApoE genes had been genotyped were selected for this study (N = 1406, aged 60-95 years). These subjects represented 80.7% of the total elderly residents in Bambuí city, MG, Brazil. Hypertension was defined as a systolic blood pressure ³140 mmHg and/or a diastolic blood pressure ³90 mmHg, or the use of anti-hypertensive medication. The exposure variable was the ApoE genotype as follows: e3 carriers,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein E; Hypertension; Low-density lipoprotein cholesterol; Triglycerides; Lipid metabolism.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200002

Cytotoxic activity of the dichloromethane fraction from Vernonia scorpioides (Lam.) Pers. (Asteraceae) against Ehrlich's tumor cells in mice
Provedor de dados: 56
Autores: Pagno,T.; Blind,L.Z.; Biavatti,M.W.; Kreuger,M.R.O.
Vernonia scorpioides has been widely used in Brazil to treat skin problems and chronic wounds, such as ulcers of the lower limbs and diabetic lesions. In the present study, we investigated the effect of a dichloromethane (DCM) fraction of V. scorpioides leaf extract on Ehrlich ascitic and solid tumor-bearing mice. The animals were treated once a day with the DCM fraction at a concentration of 5 mg/kg, administered ip during and after the development of the tumor. The lifespan, weight, number and type of leukocytes, number of tumor cells, volume of solid and ascitic tumors were measured. The development of the tumor with pre-treated tumor cells in vitro with the DCM fraction (5 mg/kg) was analyzed and the animals were sacrificed after 7 days. The DCM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vernonia scorpioides; Leaf extract; Antitumoral; Ehrlich ascitic tumor; Neutrophils.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100012

Changes in clinical and laboratory findings at the time of diagnosis of primary hyperparathyroidism in a University Hospital in São Paulo from 1985 to 2002
Provedor de dados: 56
Autores: Ohe,M.N.; Santos,R.O.; Barros,E.R.; Lage,A.; Kunii,I.S.; Abrahão,M.; Cervantes,O.; Hauache,O.M.; Lazaretti-Castro,M.; Vieira,J.G.H.
In contrast to most developed countries, most patients with primary hyperparathyroidism in Brazil are still symptomatic at diagnosis. However, we have been observing a change in this pattern, especially in the last few years. We evaluated 104 patients, 77 females and 27 males aged 11-79 years (mean: 54.4 years), diagnosed between 1985 and 2002 at a University Hospital. Diagnosis was made on the basis of clinical findings and of high total and/or ionized calcium levels, high or inappropriate levels of intact parathyroid hormone and of surgical findings in 80 patients. Patients were divided into three groups, i.e., patients diagnosed from 1985 to 1989, patients diagnosed from 1990 to 1994, and patients diagnosed from 1995 to 2002. The number of new cases...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Primary hyperparathyroidism; Hypercalcemia; Clinical presentation; Asymptomatic primary hyperparathyroidism; Parathyroid hormone; Hyperparathyroidism.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900013

Identification of microRNAs and mRNAs associated with multidrug resistance of human laryngeal cancer Hep-2 cells
Provedor de dados: 56
Autores: Yin,Wanzhong; Wang,Ping; Wang,Xin; Song,Wenzhi; Cui,Xiangyan; Yu,Hong; Zhu,Wei.
Multidrug resistance (MDR) poses a serious impediment to the success of chemotherapy for laryngeal cancer. To identify microRNAs and mRNAs associated with MDR of human laryngeal cancer Hep-2 cells, we developed a multidrug-resistant human laryngeal cancer subline, designated Hep-2/v, by exposing Hep-2 cells to stepwise increasing concentrations of vincristine (0.02-0.96'µM). Microarray assays were performed to compare the microRNA and mRNA expression profiles of Hep-2 and Hep-2/v cells. Compared to Hep-2 cells, Hep-2/v cells were more resistant to chemotherapy drugs (∼45-fold more resistant to vincristine, 5.1-fold more resistant to cisplatin, and 5.6-fold more resistant to 5-fluorouracil) and had a longer doubling time (42.33±1.76 vs 28.75±1.12'h,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multidrug resistance; Laryngeal cancer; Hep-2 cells; MRNA; MicroRNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600546

Effects of acid-base imbalance on vascular reactivity
Provedor de dados: 56
Autores: Celotto,A.C.; Capellini,V.K.; Baldo,C.F.; Dalio,M.B.; Rodrigues,A.J.; Evora,P.R.B.
Acid-base homeostasis maintains systemic arterial pH within a narrow range. Whereas the normal range of pH for clinical laboratories is 7.35-7.45, in vivo pH is maintained within a much narrower range. In clinical and experimental settings, blood pH can vary in response to respiratory or renal impairment. This altered pH promotes changes in vascular smooth muscle tone with impact on circulation and blood pressure control. Changes in pH can be divided into those occurring in the extracellular space (pHo) and those occurring within the intracellular space (pHi), although, extracellular and intracellular compartments influence each other. Consistent with the multiple events involved in the changes in tone produced by altered pHo, including type of vascular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acid-base balance; Nitric oxide; Vascular reactivity; Endothelium; Alkalosis; Acidosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600001

Expression of β-catenin and c-myc during human common bile duct development: a possible role in the morphogenesis of the common bile duct
Provedor de dados: 56
Autores: Guo,W.L.; Zhang,Q.; Wang,J.
β-catenin and c-myc play important roles in the development of tissues and organs. However, little is known about their expression patterns during the development of the human common bile duct. Immunohistochemistry was used to detect β-catenin and c-myc expression in common bile duct samples from postmortem tissues of 14 premature infants and 6 spontaneously aborted fetuses. The expression of β-catenin and c-myc was also analyzed by Western blot. The samples were divided into four groups based on the stage of human fetal development: 12, 13-27, 28-37, and &gt;37 weeks. The Image-Pro Plus v. 6.0 image analysis software was used to calculate the mean qualifying score (MQS). At fetal stages 12, 13-27, 28-37, and &gt;37 weeks, MQS of β-catenin were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-catenin; C-myc; Common bile duct; Fetal development.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700594

Personality traits and psychiatric comorbidities in alcohol dependence
Provedor de dados: 56
Autores: Donadon,M.F.; Osório,F.L.
Non-adaptive personality traits may constitute risk factors for development of psychiatric disorders such as depression and anxiety. We aim to evaluate associations and the predictive value of personality traits among alcohol-dependent individuals, with or without psychiatric comorbidities. The convenience sample comprised two groups of males over 18 years of age: one with subjects who had an alcohol dependence diagnosis (AG, n=110), and a control group without abuse and/or alcohol dependence diagnosis (CG, n=110). The groups were assessed by means of the Structured Clinical Interview DSM-IV (SCID-IV). AG participants were recruited among outpatients from the university hospital, whereas CG participants were recruited from a primary healthcare program....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alcoholism; Dependence; Personality traits; Comorbidity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100705

Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseases
Provedor de dados: 56
Autores: Sukoyan,M.A.; Kerkis,A.Y.; Mello,M.R.B.; Kerkis,I.E.; Visintin,J.A.; Pereira,L.V.
Embryonic stem cells are totipotent cells derived from the inner cell mass of blastocysts. Recently, the development of appropriate culture conditions for the differentiation of these cells into specific cell types has permitted their use as potential therapeutic agents for several diseases. In addition, manipulation of their genome in vitro allows the creation of animal models of human genetic diseases and for the study of gene function in vivo. We report the establishment of new lines of murine embryonic stem cells from preimplantation stage embryos of 129/Sv mice. Most of these cells had a normal karyotype and an XY sex chromosome composition. The pluripotent properties of the cell lines obtained were analyzed on the basis of their alkaline phosphatase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human genetic diseases; Murine embryonic stem cells; Mouse model; Transgenic mouse.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500004

Electrical parameters and water permeability properties of monolayers formed by T84 cells cultured on permeable supports
Provedor de dados: 56
Autores: Ozu,M.; Toriano,R.; Capurro,C.; Parisi,M.
T84 is an established cell line expressing an enterocyte phenotype whose permeability properties have been widely explored. Osmotic permeability (P OSM), hydraulic permeability (P HYDR) and transport-associated net water fluxes (J W-transp), as well as short-circuit current (I SC), transepithelial resistance (R T), and potential difference (deltaV T) were measured in T84 monolayers with the following results: P OSM 1.3 ± 0.1 cm.s-1 x 10-3; P HYDR 0.27 ± 0.02 cm.s-1; R T 2426 ± 109 omega.cm², and deltaV T 1.31 ± 0.38 mV. The effect of 50 µM 5,6-dichloro-1-ethyl-1,3-dihydro-2H-benzimidazol-2-one (DCEBIO), a "net Cl&lt;FONT FACE=Symbol&gt;-&lt;/FONT&gt; secretory agent", on T84 cells was also studied. We confirm the reported important increase in I SC induced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water absorption and secretion; Aquaporins; Osmotic and hydrostatic gradients; T84 cells.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100020

Enterococcus gallinarum carrying the vanA gene cluster: first report in Brazil
Provedor de dados: 56
Autores: Camargo,I.L.B.C.; Barth,A.L.; Pilger,K.; Seligman,B.G.S.; Machado,A.R.L.; Darini,A.L.C.
In 2000, Enterococcus faecalis resistant to vancomycin was first reported at a tertiary hospital in Porto Alegre, southern Brazil. The resistance spread to other hospitals and surveillance programs were established by hospital infection committees to prevent the spread of vancomycin-resistant enterococci. In February 2002, an isolate initially identified at the genus level as Enterococcus was obtained by surveillance culture (rectal swab) from a patient admitted to a hospital for treatment of septic arthritis in the shoulder. The isolate proved to be resistant to vancomycin by the disc diffusion method and confirmed by an E-test resulting in a minimal inhibitory concentration of  &gt; or = 256 µg/ml. This isolate was sent to a reference laboratory...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enterococcus gallinarum; VanA phenotype; Vancomycin; Resistance.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100012

Lipid peroxidation, detoxification capacity, and genome damage in mice after transplacental exposure to pharmaceutical drugs
Provedor de dados: 56
Autores: Markovic,D.; Katic,J.; Stojkovic,R.; Borovic,S.; Zarkovic,N.; Fucic,A.
Data on genome damage, lipid peroxidation, and levels of glutathione peroxidase (GPX) in newborns after transplacental exposure to xenobiotics are rare and insufficient for risk assessment. The aim of the current study was to analyze, in an animal model, transplacental genotoxicity, lipid peroxidation, and detoxification disturbances caused by the following drugs commonly prescribed to pregnant women: paracetamol, fluconazole, 5-nitrofurantoin, and sodium valproate. Genome damage in dams and their newborn pups transplacentally exposed to these drugs was investigated using the in vivo micronucleus (MN) assay. The drugs were administered to dams intraperitoneally in three consecutive daily doses between days 12 and 14 of pregnancy. The results were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione peroxidase; Malondialdehyde; Genome damage; Transplacental exposure.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201014

Correction Braz J Med Biol Res 1994; 27(11): 2667-2679 - Analysis of right and left ventricular performance of the rat heart with chronic myocardial infarction
Provedor de dados: 56
Autores: Stefanon,I.; Auxiliadora-Martins,M.; Vassallo,D.V.; Mill,J.G.
Tipo: Info:eu-repo/semantics/article
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900015

Hirsch's index: a case study conducted at the Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo
Provedor de dados: 56
Autores: Torro-Alves,N.; Herculano,R.D.; Terçariol,C.A.S.; Kinouchi Filho,O.; Graeff,C.F.O.
An analysis of scientific bibliographic productivity using the Hirsch h-index, information from the Institute of Scientific Information database and the Curriculum Lattes (CNPq, Brazil) was performed at the Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo (FFCLRP-USP) that has four departments in natural, biological and social sciences. Bibliometric evaluations of undergraduate programs showed a better performance of the departments of Chemistry (P &lt; 0.001) and Biology (P &lt; 0.001) when compared to the departments of Physics and Mathematics and Psychology and Education. We also analyzed the scientific output of the six graduate programs of FFCLRP: Psychology, Psychobiology, Chemistry, Physics Applied to Medicine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: H-index; Scientometrics; Science in Brazil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100013

Acute exposure to lead increases myocardial contractility independent of hypertension development
Provedor de dados: 56
Autores: Fioresi,M.; Furieri,L.B.; Simões,M.R.; Ribeiro Junior,R.F.; Meira,E.F.; Fernandes,A.A.; Stefanon,I.; Vassallo,D.V.
We studied the effects of the acute administration of small doses of lead over time on hemodynamic parameters in anesthetized rats to determine if myocardial contractility changes are dependent or not on the development of hypertension. Male Wistar rats received 320 µg/kg lead acetateiv once, and their hemodynamic parameters were measured for 2 h. Cardiac contractility was evaluated in vitro using left ventricular papillary muscles as were Na+,K+-ATPase and myosin Ca2+-ATPase activities. Lead increased left- (control: 112 ± 3.7 vs lead: 129 ± 3.2 mmHg) and right-ventricular systolic pressures (control: 28 ± 1.2vs lead: 34 ± 1.2 mmHg) significantly without modifying heart rate. Papillary muscles were exposed to 8 µM lead acetate and evaluated 60 min later....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lead; Myocardial contractility; Ventricular pressure.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200178

Two variants of HIV-1 B serotype are transmitted heterosexually in São Paulo, Brazil
Provedor de dados: 56
Autores: Casseb,J.; Hong,M.A.; Gonsalez,C.; Brígido,L.F.; Duarte,A.J.S.; Michael-Hendry,R.
HIV-1 variability may have an important impact on transmission and pathogenicity. Better characterization of the HIV epidemic in Brazil is necessary for the development of vaccine trials in this country. We analyzed sera from 108 HIV-1-infected volunteers from São Paulo City to determine serotype and reactivity for V3 motifs of HIV in this population, and the relationship to transmission mode. We concluded that the HIV-1 B serotype is frequent among heterosexually infected women, even in the absence of anal sex, and that two major V3 motifs, GPGR and GWGR, had similar prevalence among women (48% and 52%, respectively) and men (56% and 44%, respectively). We also observed an equal distribution of these strains regardless of their CD4+ T cell counts,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HIV; Serotyping; Gp 120 V3 loop; Synthetic peptides; Sexual transmission.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000002

Effects of small doses of ouabain on the arterial blood pressure of anesthetized hypertensive and normotensive rats
Provedor de dados: 56
Autores: Rossoni,L.V.; Pinto,V.D.; Vassallo,D.V.
Ouabain increases vascular resistance and may induce hypertension by inhibiting the Na+ pump. The effects of 0.18 and 18 µg/kg, and 1.8 mg/kg ouabain pretreatment on the phenylephrine (PHE; 0.1, 0.25 and 0.5 µg, in bolus)-evoked pressor responses were investigated using anesthetized normotensive (control and uninephrectomized) and hypertensive (1K1C and DOCA-salt treated) rats. Treatment with 18 µg/kg ouabain increased systolic and diastolic blood pressure in all groups studied. However, the magnitude of this increase was larger for the hypertensive 1K1C and DOCA-salt rats than for normotensive animals, while the pressor effect of 0.18 µg/kg ouabain was greater only in DOCA-salt rats. A very large dose (1.8 mg/kg) produced toxic effects on the normotensive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pressor reactivity; Ouabain; Phenylephrine; 1K1C rats; DOCA-salt; Uninephrectomy.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800014

High pressure-sensitive gene expression in Lactobacillus sanfranciscensis
Provedor de dados: 56
Autores: Vogel,R.F.; Pavlovic,M.; Hörmann,S.; Ehrmann,M.A.
Lactobacillus sanfranciscensis is a Gram-positive lactic acid bacterium used in food biotechnology. It is necessary to investigate many aspects of a model organism to elucidate mechanisms of stress response, to facilitate preparation, application and performance in food fermentation, to understand mechanisms of inactivation, and to identify novel tools for high pressure biotechnology. To investigate the mechanisms of the complex bacterial response to high pressure we have analyzed changes in the proteome and transcriptome by 2-D electrophoresis, and by microarrays and real time PCR, respectively. More than 16 proteins were found to be differentially expressed upon high pressure stress and were compared to those sensitive to other stresses. Except for one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High pressure; Lactobacillus sanfranciscensis; Proteome; Transcriptome; Stress response.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800013

Topographic distribution of the tibial somatosensory evoked potential using coherence
Provedor de dados: 56
Autores: Melges,D.B.; Infantosi,A.F.C.; Miranda de Sá,A.M.F.L.
The objective of the present study was to determine the adequate cortical regions based on the signal-to-noise ratio (SNR) for somatosensory evoked potential (SEP) recording. This investigation was carried out using magnitude-squared coherence (MSC), a frequency domain objective response detection technique. Electroencephalographic signals were collected (International 10-20 System) from 38 volunteers, without history of neurological pathology, during somatosensory stimulation. Stimuli were applied to the right posterior tibial nerve at the rate of 5 Hz and intensity slightly above the motor threshold. Response detection was based on rejecting the null hypothesis of response absence (significance level α= 0.05 and M = 500 epochs). The best detection rates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Somatosensory response detection; Magnitude-squared coherence; Tibial nerve; Topography.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200004

Strontium ranelate inhibits wear particle-induced aseptic loosening in mice
Provedor de dados: 56
Autores: Geng,Tianxiang; Sun,Shouxuan; Yu,Haochen; Guo,Haohui; Zheng,Mengxue; Zhang,Shuai; Chen,Xi; Jin,Qunhua.
The imbalance between bone formation and osteolysis plays a key role in the pathogenesis of aseptic loosening. Strontium ranelate (SR) can promote bone formation and inhibit osteolysis. The aim of this study was to explore the role and mechanism of SR in aseptic loosening induced by wear particles. Twenty wild-type (WT) female C57BL/6j mice and 20 sclerostin-/- female C57BL/6j mice were used in this study. Mice were randomly divided into four groups: WT control group, WT SR group, knockout (KO) control group, and KO SR group. We found that SR enhanced the secretion of osteocalcin (0.72±0.007 in WT control group, 0.98±0.010 in WT SR group, P=0.000), Runx2 (0.34±0.005 in WT control group, 0.47±0.010 in WT SR group, P=0.000), β-catenin (1.04±0.05 in WT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Strontium ranelate; Aseptic loosening; Sclerostin; Bone remodeling; Canonical Wnt pathway.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900605

The insulin receptor substrate 1 associates with phosphotyrosine phosphatase SHPTP2 in liver and muscle of rats
Provedor de dados: 56
Autores: Lima,M.H.M.; Zambelli,J.E.; Carvalho,C.R.O.; Saad,M.J.A.
Insulin stimulates the tyrosine kinase activity of its receptor resulting in the phosphorylation of its cytosolic substrate, insulin receptor substrate-1 (IRS-1) which, in turn, associates with proteins containing SH2 domains. It has been shown that IRS-1 associates with the tyrosine phosphatase SHPTP2 in cell cultures. While the effect of the IRS-1/SHPTP2 association on insulin signal transduction is not completely known, this association may dephosphorylate IRS-1 and may play a critical role in the mitogenic actions of insulin. However, there is no physiological demonstration of this pathway of insulin action in animal tissues. In the present study we investigated the ability of insulin to induce association between IRS-1 and SHPTP2 in liver and muscle...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Insulin action; Insulin receptor substrate; Phosphotyrosine phosphatase; SHPTP2.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100007

L1 sequence of a new human papillomavirus type-58 variant associated with cervical intraepithelial neoplasia
Provedor de dados: 56
Autores: Veras,V.S.; Cerqueira,D.M.; Martins,C.R.F.
The present study on molecular characterization of a human papillomavirus (HPV) isolated in Central Brazil describes the L1 gene sequence from a new variant of HPV-58, the isolate Bsb-02. The sample was from a smear obtained from a woman with cervical intraepithelial neoplasia grade II. The whole L1 gene from isolate Bsb-02 was sequenced automatically, showing 99.1% nucleotide identity with the gene from the HPV-58 reference. The clustering between Bsb-02 and HPV-58 reference sequence was also supported by phylogenetic analysis. Fourteen nucleotide substitutions were observed: eight were synonymous and six were associated with amino acid substitutions. A10V and V144I have not been previously described. At GenBank, the only complete L1 sequence from HPV-58...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Human papillomavirus; HPV-58; L1 gene; Variants.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100001

Using green fluorescent protein to understand the mechanisms of G-protein-coupled receptor regulation
Provedor de dados: 56
Autores: Ferguson,S.S.G.
G protein-coupled receptor (GPCR) activation is followed rapidly by adaptive changes that serve to diminish the responsiveness of a cell to further stimulation. This process, termed desensitization, is the consequence of receptor phosphorylation, arrestin binding, sequestration and down-regulation. GPCR phosphorylation is initiated within seconds to minutes of receptor activation and is mediated by both second messenger-dependent protein kinases and receptor-specific G protein-coupled receptor kinases (GRKs). Desensitization in response to GRK-mediated phosphorylation involves the binding of arrestin proteins that serve to sterically uncouple the receptor from its G protein. GPCR sequestration, the endocytosis of receptors to endosomes, not only...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arrestin; Endocytosis; Green fluorescent protein; G protein-coupled receptors; G protein-coupled receptor kinases; Resensitization.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100016

Abnormal subcellular distribution of GLUT4 protein in obese and insulin-treated diabetic female dogs
Provedor de dados: 56
Autores: Vargas,A.M.; Barros,R.P.A.; Zampieri,R.A.; Okamoto,M.M.; de Carvalho Papa,P.; Machado,U.F.
The GLUT4 transporter plays a key role in insulin-induced glucose uptake, which is impaired in insulin resistance. The objective of the present study was to investigate the tissue content and the subcellular distribution of GLUT4 protein in 4- to 12-year-old control, obese and insulin-treated diabetic mongrel female dogs (4 animals per group). The parametrial white adipose tissue was sampled and processed to obtain both plasma membrane and microsome subcellular fractions for GLUT4 analysis by Western blotting. There was no significant difference in glycemia and insulinemia between control and obese animals. Diabetic dogs showed hyperglycemia (369.9 ± 89.9 mg/dl). Compared to control, the plasma membrane GLUT4, reported per g tissue, was reduced by 55% (P...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diabetes; Obesity; Insulin resistance; GLUT4; Adipose tissue; Plasma membrane.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700020

The growing competition in Brazilian science: rites of passage, stress and burnout
Provedor de dados: 56
Autores: de Meis,L.; Velloso,A.; Lannes,D.; Carmo,M.S.; de Meis,C.
Brazil's scientific community is under pressure. Each year there is an increase in its contribution to international science and in the number of students who are trained to do research and teach at an advanced level. Most of these activities are carried out in state and federal universities, but with government funding that has decreased by more than 70% since 1996. Interviews with graduate students, post-doctoral fellows and professors in one university department with a strong research tradition illustrate the level of stress engendered by the conflict between increasing competition and diminishing resources, and serve to underscore the negative effects on creativity and on the tendency to choose science as a career.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Science funding; Public and private universities; Mental suffering; Publish or perish; Academic pressure; MSc and PhD students.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900001

Generalization of temporal order detection skill learning: two experimental studies of children with dyslexia
Provedor de dados: 56
Autores: Murphy,C.F.B.; Schochat,E.
The objective of this study was to investigate the phenomenon of learning generalization of a specific skill of auditory temporal processing (temporal order detection) in children with dyslexia. The frequency order discrimination task was applied to children with dyslexia and its effect after training was analyzed in the same trained task and in a different task (duration order discrimination) involving the temporal order discrimination too. During study 1, one group of subjects with dyslexia (N = 12; mean age = 10.9 ± 1.4 years) was trained and compared to a group of untrained dyslexic children (N = 28; mean age = 10.4 ± 2.1 years). In study 2, the performance of a trained dyslexic group (N = 18; mean age = 10.1 ± 2.1 years) was compared at three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Temporal order detection; Learning; Dyslexia; Children.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400007

The prevalence of chronic diabetic complications and metabolic syndrome is not associated with maternal type 2 diabetes
Provedor de dados: 56
Autores: Scheffel,R.S.; Kramer,C.K.; Rados,D.V.; Pinto,L.C.; Crispim,D.; Gross,J.L.; Canani,L.H.
The maternal history of type 2 diabetes mellitus (DM) has been reported more frequently in patients with type 2 DM than paternal history. The aim of the present study was to determine if there was an association between maternal history of DM and the presence of chronic complications or metabolic syndrome (MetS) in patients with type 2 DM. A cross-sectional study was conducted with 1455 patients with type 2 DM. All outpatients with type 2 diabetes attending the endocrine clinics who fulfilled the eligibility criteria were included. Familial history of DM was determined with a questionnaire. Diabetic complications were assessed using standard procedures. The definition of MetS used was that of the World Health Organization and the National Cholesterol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes mellitus; Metabolic syndrome; Microvascular chronic complications; Diabetic retinopathy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200013

Can calories from ethanol contribute to body weight preservation by malnourished rats?
Provedor de dados: 56
Autores: Aguiar,A.S.; Da-Silva,V.A.; Boaventura,G.T.
Our objective was to compare the use of calories from ethanol by well-nourished and malnourished rats in terms of body weight. Female Wistar rats weighing 170-180 g at the beginning of the study were used. The animals were divided into two groups (N = 12 each): group W received water ad libitum and group E an ethanol solution ad libitum as the only source of liquid throughout the experiment. The concentration of ethanol was increased weekly from 0 to 5, 10, 20 and 40% (v/v). In the well-nourished phase (A), all rats received food ad libitum (AW and AE). Ethanol treatment (AE) was then interrupted and water was offered to both groups. After 2 weeks both AW and AE rats were submitted to food restriction (50% of group AW food consumption), thus initiating the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ethanol; Weight gain; Malnutrition; Rats; Toxicity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600009

Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface
Provedor de dados: 56
Autores: Maranhão,A.Q.; Brígido,M.M.
We describe the expression of an anti-Z-DNA single chain variable region antibody fragment (scFv) on a filamentous phage surface. Four vectors for phage display were constructed. Two of them are able to display multiple copies of the antibody fragment, and the others can be used to make monovalent libraries. The vectors use different promoter/leader sequences to direct the expression of the fused proteins. All were able to promote the assembly of fusion virion particles. In this paper we also show the affinity selection (biopanning) of those phage-antibodies based on the capacity of their products to recognize the antigen. We used biotinylated Z-DNA and the selection was performed in a solution phase fashion. The data presented here indicate that these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-nucleic acid antibodies; Phage display; Biopanning; ScFv; Z-DNA.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500012

Cloning, expression and purification of recombinant streptokinase: partial characterization of the protein expressed in Escherichia coli
Provedor de dados: 56
Autores: Avilán,L.; Yarzábal,A.; Jürgensen,C.; Bastidas,M.; Cruz,J.; Puig,J.
We cloned the streptokinase (STK) gene of Streptococcus equisimilis in an expression vector of Escherichia coli to overexpress the profibrinolytic protein under the control of a tac promoter. Almost all the recombinant STK was exported to the periplasmic space and recovered after gentle lysozyme digestion of induced cells. The periplasmic fraction was chromatographed on DEAE Sepharose followed by chromatography on phenyl-agarose. Active proteins eluted between 4.5 and 0% ammonium sulfate, when a linear gradient was applied. Three major STK derivatives of 47.5 kDa, 45 kDa and 32 kDa were detected by Western blot analysis with a polyclonal antibody. The 32-kDa protein formed a complex with human plasminogen but did not exhibit Glu-plasminogen activator...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recombinant streptokinase; Plasminogen activators; Protein purification.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200007

Alternagin-C, a disintegrin-like protein from the venom of Bothrops alternatus, modulates alpha2ß1 integrin-mediated cell adhesion, migration and proliferation
Provedor de dados: 56
Autores: Selistre-de-Araujo,H.S.; Cominetti,M.R.; Terruggi,C.H.B.; Mariano-Oliveira,A.; De Freitas,M.S.; Crepin,M.; Figueiredo,C.C.; Morandi,V.
The alpha2ß1 integrin is a major collagen receptor that plays an essential role in the adhesion of normal and tumor cells to the extracellular matrix. Alternagin-C (ALT-C), a disintegrin-like protein purified from the venom of the Brazilian snake Bothrops alternatus, competitively interacts with the alpha2ß1 integrin, thereby inhibiting collagen binding. When immobilized in plate wells, ALT-C supports the adhesion of fibroblasts as well as of human vein endothelial cells (HUVEC) and does not detach cells previously bound to collagen I. ALT-C is a strong inducer of HUVEC proliferation in vitro. Gene expression analysis was done using an Affimetrix HU-95A probe array with probe sets of ~10,000 human genes. In human fibroblasts growing on collagen-coated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha2ß1 integrin; Disintegrin; Snake venom; Adhesion; Gene expression; Extracellular matrix.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000007

Proteomic analysis of cytosolic proteins associated with petite mutations in Candida glabrata
Provedor de dados: 56
Autores: Loureiro y Penha,C.V.; Kubitschek,P.H.B.; Larcher,G.; Perales,J.; Rodriguez León,I.; Lopes-Bezerra,L.M.; Bouchara,J.P.
The incidence of superficial or deep-seated infections due to Candida glabrata has increased markedly, probably because of the low intrinsic susceptibility of this microorganism to azole antifungals and its relatively high propensity to acquire azole resistance. To determine changes in the C. glabrata proteome associated with petite mutations, cytosolic extracts from an azole-resistant petite mutant of C. glabrata induced by exposure to ethidium bromide, and from its azole-susceptible parent isolate were compared by two-dimensional polyacrylamide gel electrophoresis. Proteins of interest were identified by peptide mass fingerprinting or sequence tagging using a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer. Tryptic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida glabrata; Petite mutations; Cytosolic extracts; Proteomic analysis; Azole resistance.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200010

The role of nitric oxide in reproduction
Provedor de dados: 56
Autores: McCann,S.M.; Mastronardi,C.; Walczewska,A.; Karanth,S.; Rettori,V.; Yu,W.H.
Nitric oxide (NO) plays a crucial role in reproduction at every level in the organism. In the brain, it activates the release of luteinizing hormone-releasing hormone (LHRH). The axons of the LHRH neurons project to the mating centers in the brain stem and by afferent pathways evoke the lordosis reflex in female rats. In males, there is activation of NOergic terminals that release NO in the corpora cavernosa penis to induce erection by generation of cyclic guanosine monophosphate (cGMP). NO also activates the release of LHRH which reaches the pituitary and activates the release of gonadotropins by activating neural NO synthase (nNOS) in the pituitary gland. In the gonad, NO plays an important role in inducing ovulation and in causing luteolysis, whereas in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NO and synthase; NG-monomethyl-L-arginine; Nitroprusside; NOergic neurons; FSH; LH; LHRH; FSHRF.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100007

Quantitative evaluation of experimental choroidal neovascularization by confocal scanning laser ophthalmoscopy: fluorescein angiogram parallels heparan sulfate proteoglycan expression
Provedor de dados: 56
Autores: Regatieri,C.V.; Dreyfuss,J.L.; Melo,G.B.; Lavinsky,D.; Hossaka,S.K.; Rodrigues,E.B.; Farah,M.E.; Maia,M.; Nader,H.B.
The objective of the present study was to develop a quantitative method to evaluate laser-induced choroidal neovascularization (CNV) in a rat model using Heidelberg Retina Angiograph 2 (HRA2) imaging. The expression of two heparan sulfate proteoglycans (HSPG) related to inflammation and angiogenesis was also investigated. CNV lesions were induced with argon laser in 21 heterozygous Zucker rats and after three weeks a fluorescein angiogram and autofluorescence exams were performed using HRA2. The area and greatest linear dimension were measured by two observers not aware of the protocol. Bland-Altman plots showed agreement between the observers, suggesting that the technique was reproducible. After fluorescein angiogram, HSPG (perlecan and syndecan-4) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Choroidal neovascularization; Fluorescein angiogram; Proteoglycans; Angiogenesis; Confocal scanning laser ophthalmoscope.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700004

The relationship between salivary cortisol concentrations and anxiety in adolescent and non-adolescent pregnant women
Provedor de dados: 56
Autores: Rondó,P.H.C.; Vaz,A.J.; Moraes,F.; Tomkins,A.
The main purpose of the present study was to determine the relationship between salivary cortisol concentrations and self-report anxiety in 50 adolescent and 178 non-adolescent women during the last month of pregnancy. The subjects were randomly selected from a previous study involving women who attended antenatal care from September 1997 to August 2000 at 17 health services in Southeast Brazil. Salivary cortisol was measured with an enzyme immunoassay kit, and anxiety was assessed by the State-Trait Anxiety Inventories (STAI) of Spielberger. After saliva collection, the participants completed the STAI. Mean concentrations of cortisol for both pregnant adolescents (14.17 ± 6.78 nmol/l) and non-adolescents (13.81 ± 8.51 nmol/l) were similar (P = 0.89)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anxiety; Salivary cortisol; Pregnant adolescents; State Anxiety Inventory.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900016

Effect of point mutations on Herbaspirillum seropedicae NifA activity
Provedor de dados: 56
Autores: Aquino,B.; Stefanello,A.A.; Oliveira,M.A.S.; Pedrosa,F.O.; Souza,E.M.; Monteiro,R.A.; Chubatsu,L.S.
NifA is the transcriptional activator of the nif genes in Proteobacteria. It is usually regulated by nitrogen and oxygen, allowing biological nitrogen fixation to occur under appropriate conditions. NifA proteins have a typical three-domain structure, including a regulatory N-terminal GAF domain, which is involved in control by fixed nitrogen and not strictly required for activity, a catalytic AAA+ central domain, which catalyzes open complex formation, and a C-terminal domain involved in DNA-binding. In Herbaspirillum seropedicae, a β-proteobacterium capable of colonizing Graminae of agricultural importance, NifA regulation by ammonium involves its N-terminal GAF domain and the signal transduction protein GlnK. When the GAF domain is removed, the protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; Herbaspirillum seropedicae; NifA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800683

Urinary semaphorin 3A as an early biomarker to predict contrast-induced acute kidney injury in patients undergoing percutaneous coronary intervention
Provedor de dados: 56
Autores: Ning,Li; Li,Zhiguo; Wei,Dianjun; Chen,Haiyan; Yang,Chao; Wu,Dawei; Wang,Yanchun; Zhang,Jingwei.
Contrast-induced acute kidney injury (CI-AKI) is a serious complication of diagnostic coronary angiograph and percutaneous coronary intervention (PCI). However, the exact pathophysiological mechanisms underlying CI-AKI development are largely unknown. The present study examined whether urinary semaphorin 3A levels predict the development of CI-AKI in patients undergoing PCI. This study enrolled 168 patients with stable angina undergoing elective PCI. Serial urine samples, obtained at baseline and 2, 6, 12, 24, 36, and 48 h post-PCI were analyzed by semaphorin 3A and neutrophil gelatinase-associated lipocalin (NGAL) ELISA kit. AKI was defined as an increase in serum creatinine beyond 50% according to the RIFLE classification system. Receiver operator...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contrast-induced acute kidney injury; Percutaneous coronary intervention; Semaphorin 3A; Neutrophil gelatinase-associated lipocalin; Biomarker.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400618

2-Bromo-1,4-naphthoquinone: a potentially improved substitute of menadione in Apatone™ therapy
Provedor de dados: 56
Autores: Graciani,F.S.; Ximenes,V.F.
Apatone™, a combination of menadione (2-methyl-1,4-naphthoquinone, VK3) and ascorbic acid (vitamin C, VC) is a new strategy for cancer treatment. Part of its effect on tumor cells is related to the cellular pro-oxidative imbalance provoked by the generation of hydrogen peroxide (H2O2) through naphthoquinone redox cycling. In this study, we attempted to find new naphthoquinone derivatives that would increase the efficiency of H2O2 production, thereby potentially increasing its efficacy for cancer treatment. The presence of an electron-withdrawing group in the naphthoquinone moiety had a direct effect on the efficiency of H2O2 production. The compound 2-bromo-1,4-naphthoquinone (BrQ), in which the bromine atom substituted the methyl group in VK3, was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer Apatone™ Ascorbic acid Hydrogen peroxide 2-Bromo-1; 4-naphthoquinone.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800003

Intercostal and forearm muscle deoxygenation during respiratory fatigue in patients with heart failure: potential role of a respiratory muscle metaboreflex
Provedor de dados: 56
Autores: Moreno,A.M.; Castro,R.R.T.; Silva,B.M.; Villacorta,H.; Junior,M. Sant'Anna; Nóbrega,A.C.L.
The purpose of this study was to determine the effect of respiratory muscle fatigue on intercostal and forearm muscle perfusion and oxygenation in patients with heart failure. Five clinically stable heart failure patients with respiratory muscle weakness (age, 66±12 years; left ventricle ejection fraction, 34±3%) and nine matched healthy controls underwent a respiratory muscle fatigue protocol, breathing against a fixed resistance at 60% of their maximal inspiratory pressure for as long as they could sustain the predetermined inspiratory pressure. Intercostal and forearm muscle blood volume and oxygenation were continuously monitored by near-infrared spectroscopy with transducers placed on the seventh left intercostal space and the left forearm. Data were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Metaboreflex; Muscles respiratory.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100972

Silencing of augmenter of liver regeneration inhibited cell proliferation and triggered apoptosis in U266 human multiple myeloma cells
Provedor de dados: 56
Autores: Zeng,H.Q.; Luo,Y.; Lou,S.F.; Liu,Q.; Zhang,L.; Deng,J.C.
Augmenter of liver regeneration (ALR) is a thermostable cytokine that was originally identified to promote the growth of hepatocytes. This study was conducted to explore the expression and function of ALR in multiple myeloma (MM), a common hematologic malignancy. Real-time PCR and western blot analysis were performed to detect the expression of ALR in U266 human MM cells and healthy peripheral blood mononuclear cells (PBMCs). U266 MM cells were exposed to 20 or 40 μg/mL of recombinant ALR and tested for cell proliferation. Small interfering RNA-mediated silencing of ALR was done to investigate the role of ALR in cell proliferation, apoptosis, and cytokine production. Compared to PBMCs, U266 MM cells exhibited significantly higher levels of ALR at both the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Augmenter of liver regeneration; Hematologic cancer; Proliferation; Small interfering RNA.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000611

Inflammatory and anti-inflammatory effects of soybean agglutinin
Provedor de dados: 56
Soybean agglutinin (SBA) lectin, a protein present in raw soybean meals, can bind to and be extensively endocytosed by intestinal epithelial cells, being nutritionally toxic for most animals. In the present study we show that SBA (5-200 µg/cavity) injected into different cavities of rats induced a typical inflammatory response characterized by dose-dependent exudation and neutrophil migration 4 h after injection. This effect was blocked by pretreatment with glucocorticoid (0.5 mg/kg) or by co-injection of N-acetyl-galactosamine (100 x [M] lectin), but not of other sugars (100 x [M] lectin), suggesting an inflammatory response related to the lectin activity. Neutrophil accumulation was not dependent on a direct effect of SBA on the macrophage population...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lectin; Soybean agglutinin (SBA); Neutrophil migration; Inflammation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700009

Influence of a multideficient diet from northeastern Brazil on resting blood pressure and baroreflex sensitivity in conscious, freely moving rats
Provedor de dados: 56
Autores: Monteiro,F.M.F.; Lahlou,S.; Albuquerque,J.A.; Cabral,A.M.S.
The "regional basic diet" or RBD is a multideficient diet (providing 8% protein) which is known to produce dietary deficiencies in some populations in northeastern Brazil. The present study investigated the effects of RBD-induced malnutrition on resting blood pressure and baroreflex sensitivity in conscious rats. Malnourished rats were obtained by feeding dams the RBD during mating and pregnancy (RBD-1 group) or during nursing and a 10-day period after weaning (RBD-2 group). At 90 days of age, only RBD-2 rats weighed significantly (P&lt;0.001) less than control rats born to dams fed a standard commercial diet (23% protein) during pregnancy and nursing. Baseline mean arterial pressure and heart rate of both RBD-1 and RBD-2 rats were comparable to those of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multideficient diet; Intrauterine and postnatal malnutrition; Blood pressure; Baroreflex sensitivity; Conscious rats.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200017

Relationship between Brazilian airline pilot errors and time of day
Provedor de dados: 56
Autores: de Mello,M.T.; Esteves,A.M.; Pires,M.L.N.; Santos,D.C.; Bittencourt,L.R.A.; Silva,R.S.; Tufik,S.
Flight safety is one of the most important and frequently discussed issues in aviation. Recent accident inquiries have raised questions as to how the work of flight crews is organized and the extent to which these conditions may have been contributing factors to accidents. Fatigue is based on physiologic limitations, which are reflected in performance deficits. The purpose of the present study was to provide an analysis of the periods of the day in which pilots working for a commercial airline presented major errors. Errors made by 515 captains and 472 copilots were analyzed using data from flight operation quality assurance systems. To analyze the times of day (shifts) during which incidents occurred, we divided the light-dark cycle (24:00) in four...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Airline pilot errors; Circadian rhythm.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200014

Tissue-specific regulation of IRS-1 in unilaterally nephrectomized rats
Provedor de dados: 56
Autores: Sasse,A.D.; Chen,E.; Carvalho,C.R.O.; Gontijo,J.A.R.; Brenelli,S.L.; Saad,M.J.A.
Insulin stimulates the tyrosine kinase activity of its receptor, resulting in the phosphorylation of its cytosolic substrate, insulin receptor substrate 1 (IRS-1). IRS-1 is also a substrate for different peptides and growth factors, and a transgenic mouse "knockout" for this protein does not have normal growth. However, the role of IRS-1 in kidney hypertrophy and/or hyperplasia was not investigated. In the present study we investigated IRS-1 protein and tyrosine phosphorylation levels in the remnant kidney after unilateral nephrectomy (UNX) in 6-week-old male Wistar rats. After insulin stimulation the levels of insulin receptor and IRS-1 tyrosine phosphorylation were reduced to 79 ± 5% (P&amp;lt;0.005) and 58 ± 6% (P&amp;lt;0.0001), respectively, of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin receptor; Insulin receptor substrate; Unilateral nephrectomy; Insulin action.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000004

The bradycardic and hypotensive responses to serotonin are reduced by activation of GABA A receptors in the nucleus tractus solitarius of awake rats
Provedor de dados: 56
Autores: Callera,J.C.; Colombari,E.; De Luca Jr.,L.A.; Menani,J.V.
We investigated the effects of bilateral injections of the GABA receptor agonists muscimol (GABA A) and baclofen (GABA B) into the nucleus tractus solitarius (NTS) on the bradycardia and hypotension induced by iv serotonin injections (5-HT, 2 µg/rat) in awake male Holtzman rats. 5-HT was injected in rats with stainless steel cannulas implanted bilaterally in the NTS, before and 5, 15, and 60 min after bilateral injections of muscimol or baclofen into the NTS. The responses to 5-HT were tested before and after the injection of atropine methyl bromide. Muscimol (50 pmol/50 nl, N = 8) into the NTS increased basal mean arterial pressure (MAP) from 115 ± 4 to 144 ± 6 mmHg, did not change basal heart rate (HR) and reduced the bradycardia (-40 ± 14 and -73 ± 26...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscimol; GABA receptors; Nucleus tractus solitarius; Parasympathetic component; Bezold-Jarisch reflex; Awake rats.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700016

Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality
Provedor de dados: 56
Autores: Eskiocak,S.; Gozen,A.S.; Taskiran,A.; Kilic,A.S.; Eskiocak,M.; Gulen,S.
It has been reported that mental stress causes abnormality of spermiogram parameters. We investigated the effect of psychological stress on the L-arginine-nitric oxide (NO) pathway. Semen samples were collected from 29 healthy fourth semester medical students just before (stress) and 3 months after (non-stress) the final examinations. Psychological stress was measured by the State Anxiety Inventory questionnaire. After standard semen analysis, arginase activity and NO concentration were measured spectrophotometrically in the seminal plasma. Measurements were made in duplicate. During the stress period, sperm concentration (41.28 ± 3.70 vs 77.62 ± 7.13 x 10(6)/mL), rapid progressive motility of spermatozoa (8.79 ± 1.66 vs 20.86 ± 1.63%) and seminal plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psychological stress; Arginase; Nitric oxide; Sperm motility; Sperm count.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500003

Surfactant protein B gene polymorphism in preterm babies with respiratory distress syndrome
Provedor de dados: 56
Autores: Lyra,P.P.R.; Diniz,E.M.A.; Abe-Sandes,K.; Angelo,A.L.D.; Machado,T.M.B.; Cardeal,M.
The etiology of respiratory distress syndrome (RDS) is multifactorial and multigenic. Studies have suggested that polymorphisms and mutations in the surfactant protein B (SP-B) gene are associated with the pathogenesis of RDS. The objectives of this study were to determine and compare the frequencies of SP-B gene polymorphisms in preterm babies with and without RDS. We studied 151 neonates: 79 preterm babies without RDS and 72 preterm newborns with RDS. The following four SP-B gene polymorphisms were analyzed: A/C at -18, C/T at 1580, A/G at 9306, and G/C at nucleotide 8714. The polymorphisms were detected by PCR amplification of genomic DNA and genotyping. The genotypes were determined using PCR-based converted restriction fragment length polymorphisms....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Newborn respiratory distress syndrome; Genetic polymorphism; Surfactant protein B.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100010

Memory mood congruency phenomenon in bipolar I disorder and major depression disorder patients
Provedor de dados: 56
Autores: Delgado,V.B.; Kapczinski,F.; Chaves,M.L.F.
The objective of the present study was to evaluate memory performance in tasks with and without affective content (to confirm the mood congruency phenomenon) in acutely admitted patients with bipolar I disorder (BD) and major depression disorder (MDD) and in healthy participants. Seventy-eight participants (24 BD, 29 MDD, and 25 healthy controls) were evaluated. Three word lists were used as the memory task with affective content (positive, negative and indifferent). Psychiatric symptoms were also evaluated with rating scales (Young Mania Rating Scale for mania and Hamilton Depression Rating Scale for depression). Patients were selected during the first week of hospitalization. BD patients showed higher scores in the word span with positive tone than MDD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Memory; Bipolar disorder; Depression; Affect.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900010

Inducing mutations in the mouse genome with the chemical mutagen ethylnitrosourea
Provedor de dados: 56
Autores: Massironi,S.M.G.; Reis,B.L.F.S.; Carneiro,J.G.; Barbosa,L.B.S.; Ariza,C.B.; Santos,G.C.; Guénet,J.L.; Godard,A.L.B.
When compared to other model organisms whose genome is sequenced, the number of mutations identified in the mouse appears extremely reduced and this situation seriously hampers our understanding of mammalian gene function(s). Another important consequence of this shortage is that a majority of human genetic diseases still await an animal model. To improve the situation, two strategies are currently used: the first makes use of embryonic stem cells, in which one can induce knockout mutations almost at will; the second consists of a genome-wide random chemical mutagenesis, followed by screening for mutant phenotypes and subsequent identification of the genetic alteration(s). Several projects are now in progress making use of one or the other of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mouse; Ethylnitrosourea; Mutation; Co-isogenic mutations; Mutagenesis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900009

Molecular and cellular pathogenesis of autosomal dominant polycystic kidney disease
Provedor de dados: 56
Autores: Bastos,A.P.; Onuchic,L.F.
Autosomal dominant polycystic kidney disease (ADPKD) is one of the most common human life-threatening monogenic disorders. The disease is characterized by bilateral, progressive renal cystogenesis and cyst and kidney enlargement, often leading to end-stage renal disease, and may include extrarenal manifestations. ADPKD is caused by mutation in one of two genes, PKD1 and PKD2, which encode polycystin-1 (PC1) and polycystin-2 (PC2), respectively. PC2 is a non-selective cation channel permeable to Ca2+, while PC1 is thought to function as a membrane receptor. The cyst cell phenotype includes increased proliferation and apoptosis, dedifferentiation, defective planar polarity, and a secretory pattern associated with extracellular matrix remodeling. The two-hit...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autosomal dominant polycystic kidney disease; PKD1 gene; PKD2 gene; Polycystins 1 and 2; Cystogenesis; Primary cilium.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700001

Tai Chi exercise and functional electrical stimulation of lower limb muscles for rehabilitation in older adults with chronic systolic heart failure: a non-randomized clinical trial
Provedor de dados: 56
Autores: Hao,Yi; Zhang,Long; Zhang,Zhenhua; Chen,Lin; He,Ning; Zhu,Shuai.
Exercise-based training decreases hospitalizations in heart failure patients but such patients have exercise intolerance. The objectives of the study were to evaluate the effect of 12 weeks of Tai Chi exercise and lower limb muscles' functional electrical stimulation in older chronic heart failure adults. A total of 1,084 older adults with chronic systolic heart failure were included in a non-randomized clinical trial (n=271 per group). The control group did not receive any kind of intervention, one group received functional electrical stimulation of lower limb muscles (FES group), another group practiced Tai Chi exercise (TCE group), and another received functional electrical stimulation of lower limb muscles and practiced Tai Chi exercise (FES &amp; TCE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Geriatric; Heart failure; Kansas City Cardiomyopathy Questionnaire; Functional electrical stimulation of lower limb muscles; Tai Chi exercise.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200604

Expression of BAFF and BR3 in patients with systemic lupus erythematosus
Provedor de dados: 56
Autores: Duan,J.H.; Jiang,Y.; Mu,H.; Tang,Z.Q.
The objective of this study was to examine the relationship between the expression of B cell activating factor (BAFF) and BAFF receptor in patients with disease activity of systemic lupus erythematosus (SLE). Real-time RT-PCR was used to examine BAFF mRNA expression in peripheral blood monocytes of active and stable SLE patients and healthy controls. The percentage of BAFF receptor 3 (BR3) on B lymphocytes was measured by flow cytometry. Soluble BAFF levels in serum were assayed by ELISA. Microalbumin levels were assayed by an automatic immune analysis machine. BAFF mRNA and soluble BAFF levels were highest in the active SLE group, followed by the stable SLE group, and controls (P&lt;0.01). The percentage of BR3 on B lymphocytes was downregulated in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic lupus erythematosus; Peripheral blood mononuclear cells; B cell-activating factor; BAFF receptor.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300706

Induction of cell-mediated immunity during early stages of infection with intracellular protozoa
Provedor de dados: 56
Autores: Gazzinelli,R.T.; Talvani,A.; Camargo,M.M.; Santiago,H.C.; Oliveira,M.A.P.; Vieira,L.Q.; Martins,G.A.; Aliberti,J.C.S.; Silva,J.S.
Toxoplasma gondii and Trypanosoma cruzi are intracellular parasites which, as part of their life cycle, induce a potent cell-mediated immunity (CMI) maintained by Th1 lymphocytes and IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;. In both cases, induction of a strong CMI is thought to protect the host against rapid parasite multiplication and consequent pathology and lethality during the acute phase of infection. However, the parasitic infection is not eliminated by the immune system and the vertebrate host serves as a parasite reservoir. In contrast, Leishmania sp, which is a slow growing parasite, appears to evade induction of CMI during early stages of infection as a strategy for surviving in a hostile environment (i.e., inside the macrophages which are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Toxoplasma gondii; Trypanosoma cruzi; Macrophages; Cytokines; Nitric oxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100012

Effect of trimetazidine treatment on the transient outward potassium current of the left ventricular myocytes of rats with streptozotocin-induced type 1 diabetes mellitus
Provedor de dados: 56
Autores: Xiang,Yu-luan; He,Li; Xiao,Jun; Xia,Shuang; Deng,Song-bai; Xiu,Yun; She,Qiang.
Cardiovascular complications are a leading cause of mortality in patients with diabetes mellitus (DM). The present study was designed to investigate the effects of trimetazidine (TMZ), an anti-angina drug, on transient outward potassium current (Ito) remodeling in ventricular myocytes and the plasma contents of free fatty acid (FFA) and glucose in DM. Sprague-Dawley rats, 8 weeks old and weighing 200-250 g, were randomly divided into three groups of 20 animals each. The control group was injected with vehicle (1 mM citrate buffer), the DM group was injected with 65 mg/kg streptozotocin (STZ) for induction of type 1 DM, and the DM + TMZ group was injected with the same dose of STZ followed by a 4-week treatment with TMZ (60 mg·kg-1·day-1). All animals were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transient outward K+ current; Free fatty acid; Trimetazidine; Diabetic cardiomyopathy; Channel protein; Ventricular myocytes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300004

Combined effect of short stature and socioeconomic status on body mass index and weight gain during reproductive age in Brazilian women
Provedor de dados: 56
Autores: Sichieri,R.; Silva,C.V.C.; Moura,A.S.
Short stature, a marker for undernutrition early in life, has been associated with obesity in Brazilian women, but not in men. We tested the hypothesis that weight gain during the reproductive years could explain this gender difference. A national two-stage household survey of mothers with one or more children under five years of age was conducted in Brazil in 1996. The subjects were women aged 20 to 45 years (N = 2297), with last delivery seven months or more prior to the interview. The regions of the country were divided into rural, North/Northeast (urban underdeveloped) and South/Southeast/Midwest (urban developed). The dependent variables were current body mass index (BMI) measured, BMI prior to childbearing (reported), and BMI change. Socioeconomic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parity; Body mass index; Weight gain; Pregnancy; Overweight; Stature.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000007

Semi-automatic measurement of visual verticality perception in humans reveals a new category of visual field dependency
Provedor de dados: 56
Autores: Kaleff,C.R.; Aschidamini,C.; Baron,J.; Leone,C.N. de; Canavarro,S.; Vargas,C.D.
Previous assessment of verticality by means of rod and rod and frame tests indicated that human subjects can be more (field dependent) or less (field independent) influenced by a frame placed around a tilted rod. In the present study we propose a new approach to these tests. The judgment of visual verticality (rod test) was evaluated in 50 young subjects (28 males, ranging in age from 20 to 27 years) by randomly projecting a luminous rod tilted between -18 and +18° (negative values indicating left tilts) onto a tangent screen. In the rod and frame test the rod was displayed within a luminous fixed frame tilted at +18 or -18°. Subjects were instructed to verbally indicate the rod’s inclination direction (forced choice). Visual dependency was estimated by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rod test; Rod and frame test; Subjective visual vertical; Visual streams; Two-alternative forced-choice task.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800004

Gastrodin induced HO-1 and Nrf2 up-regulation to alleviate H2O2-induced oxidative stress in mouse liver sinusoidal endothelial cells through p38 MAPK phosphorylation
Provedor de dados: 56
Autores: Zhang,Hongbin; Yuan,Bo; Huang,Hanfei; Qu,Siming; Yang,Shikun; Zeng,Zhong.
Nuclear factor erythroid-related factor 2 (Nrf2) has been implicated in several detoxifying and antioxidant defense processes. Nrf2-mediated heme oxygenase-1 (HO-1) expression was demonstrated to play a key role against oxidative stress. Gastrodin (GSTD) is a well-known active compound isolated from the roots of Rhizoma gastrodiae, a plant used in ancient Chinese traditional medicine. The aim of this work was to investigate whether GSTD could alleviate H2O2-induced oxidative stress in mouse liver sinusoidal endothelial cells (LSECs). In LSECs exposed to 1 mM H2O2, treatment with GSTD (1, 10, or 50 µM) resulted in higher cell viability than the untreated control. Treated cells maintained a higher Bcl2/Bax ratio and suppressed caspase-9 expression compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastrodin; Liver sinusoidal endothelial cells; Heme oxygenase 1; Oxidative stress; Hydrogen peroxide.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000611

Effect of ryanodine on sinus node recovery time determined in vitro
Provedor de dados: 56
Autores: Bassani,J.W.M.; Godoy,C.M.G.; Bassani,R.A.
Evidence has indicated that the sarcoplasmic reticulum (SR) might be involved in the generation of spontaneous electrical activity in atrial pacemaker cells. We report the effect of disabling the SR with ryanodine (0.1 µM) on the sinus node recovery time (SNRT) measured in isolated right atria from 4-6-month-old male Wistar rats. Electrogram and isometric force were recorded at 36.5oC. Two methods for sinus node resetting were used: a) pulse: a single stimulus pulse interpolated at coupling intervals of 50, 65 or 80% of the regular spontaneous cycle length (RCL), and b) train: a 2-min train of pulses at intervals of 50, 65 or 80% of RCL. Corrected SNRT (cSNRT) was calculated as the difference between SNRT (first spontaneous cycle length after stimulation...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sinus node recovery time; Overdrive suppression; Electrical stimulation; Calcium; Sarcoplasmic reticulum; Ryanodine.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800015

T cell subsets and immunoglobulin G levels are associated with the infection status of systemic lupus erythematosus patients
Provedor de dados: 56
Autores: Wu,Lifen; Wang,Xinru; Chen,Fenghua; Lv,Xing; Sun,Wenwen; Guo,Ying; Hou,Hou; Ji,Haiyan; Wei,Wei; Gong,Lu.
Systemic lupus erythematosus (SLE) is a chronic, autoimmune disorder that affects nearly all organs and tissues. As knowledge about the mechanism of SLE has increased, some immunosuppressive agents have become routinely used in clinical care, and infections have become one of the direct causes of mortality in SLE patients. To identify the risk factors indicative of infection in SLE patients, a case control study of our hospital's medical records between 2011 and 2013 was performed. We reviewed the records of 117 SLE patients with infection and 61 SLE patients without infection. Changes in the levels of T cell subsets, immunoglobulin G (IgG), complement C3, complement C4, globulin, and anti-double-stranded DNA (anti-ds-DNA) were detected. CD4+ and CD4+/CD8+...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic lupus erythematosus; Infection; T cell subsets; Immunoglobulin G.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200610

5-Bromo-2’-deoxyuridine induces visible morphological alteration in the DNA puffs of the anterior salivary gland region of Bradysia hygida (Diptera, Sciaridae)
Provedor de dados: 56
Autores: de Almeida,J.C.; Sauaia,H.; Viana,J.C.
5-Bromo-2’-deoxyuridine (BrdUrd) has long been known to interfere with cell differentiation. We found that treatment ofBradysia hygida larvae with BrdUrd during DNA puff anlage formation in the polytene chromosomes of the salivary gland S1 region noticeably affects anlage morphology. However, it does not affect subsequent metamorphosis to the adult stage. The chromatin of the chromosomal sites that would normally form DNA puffs remains very compact and DNA puff expansion does not occur with administration of 4 to 8 mM BrdUrd. Injection of BrdUrd at different ages provoked a gradient of compaction of the DNA puff chromatin, leading to the formation of very small to almost normal puffs. By immunodetection, we show that the analogue is preferentially...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA puffs; BrdUrd; Gene amplification; Polytene chromosomes; Salivary glands; Sciaridae.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200002

A tribute to Samuel MacDonald McCann (1925-2007): in honor of one of the last pioneers of Neuroendocrinology
Provedor de dados: 56
Autores: Antunes-Rodrigues,J.; Bedran de Castro,J.C.; Franci,C.R.; Anselmo Franci,J.A.; Pazos Moura,C.C.; Moura,E.G.; Marubayashi,U.
Tipo: Info:eu-repo/semantics/other
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500013

Brain ischemia alters platelet ATP diphosphohydrolase and 5'-nucleotidase activities in naive and preconditioned rats
Provedor de dados: 56
Autores: Frassetto,S.S.; Schetinger,M.R.C.; Schierholt,R.; Webber,A.; Bonan,C.D.; Wyse,A.T.; Dias,R.D.; Netto,C.A.; Sarkis,J.J.F.
The effects of transient forebrain ischemia, reperfusion and ischemic preconditioning on rat blood platelet ATP diphosphohydrolase and 5'-nucleotidase activities were evaluated. Adult Wistar rats were submitted to 2 or 10 min of single ischemic episodes, or to 10 min of ischemia 1 day after a 2-min ischemic episode (ischemic preconditioning) by the four-vessel occlusion method. Rats submitted to single ischemic insults were reperfused for 60 min and for 1, 2, 5, 10 and 30 days after ischemia; preconditioned rats were reperfused for 60 min 1 and 2 days after the long ischemic episode. Brain ischemia (2 or 10 min) inhibited ATP and ADP hydrolysis by platelet ATP diphosphohydrolase. On the other hand, AMP hydrolysis by 5'-nucleotidase was increased after 2,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain ischemia; Ischemic preconditioning; Rat platelets; ATP diphosphohydrolase; 5'-nucleotidase.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100017

Cloning, sequence analysis, and expression of cDNA coding for the major house dust mite allergen, Der f 1, in Escherichia coli
Provedor de dados: 56
Autores: Cui,Y.; Zhou,P.; Peng,J.; Peng,M.; Zhou,Y.; Lin,Y.; Liu,L.
Our objective was to clone, express and characterize adult Dermatophagoides farinae group 1 (Der f 1) allergens to further produce recombinant allergens for future clinical applications in order to eliminate side reactions from crude extracts of mites. Based on GenBank data, we designed primers and amplified the cDNA fragment coding for Der f 1 by nested-PCR. After purification and recovery, the cDNA fragment was cloned into the pMD19-T vector. The fragment was then sequenced, subcloned into the plasmid pET28a(+), expressed in Escherichia coli BL21 and identified by Western blotting. The cDNA coding for Der f 1 was cloned, sequenced and expressed successfully. Sequence analysis showed the presence of an open reading frame containing 966 bp that encodes a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatophagoides farinae; Der f 1; Recombinant allergens; Cloning; Expression; Bioinformatics.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500006

A novel homozygous mutation in the solute carrier family 12 member 3 gene in a Chinese family with Gitelman syndrome
Provedor de dados: 56
Autores: Zhang,Y.; Zhang,F.; Chen,D.; Lü,Q.; Tang,L.; Yang,C.; Lei,M.; Tong,N.
Loss of function of mutated solute carrier family 12 member 3 (SLC12A3) gene is the most frequent etiology for Gitelman syndrome (GS), which is mainly manifested by hypokalemia, hypomagnesemia and hypocalciuria. We report the genetic characteristics of one suspicious Chinese GS pedigree by gene sequencing. Complete sequencing analysis of the SLC12A3 gene revealed that both the proband and his elder sister had a novel homozygous SLC12A3 mutation: c.2099T&gt;C and p.Leu700Pro. Moreover, the SLC12A3 genes of his mother and daughter encoded the same mutated heterozygote. It was noted that in this pedigree, only the proband complained about recurrent episodes of bilateral lower limb weakness over 8 years, while his elder sister, mother and daughter did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gitelman syndrome; SLC12A3 gene; Homozygous mutant; Pedigree.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100701

The X-X-/E+E+ genotype of the XbaI/EcoRI polymorphisms of the apolipoprotein B gene as a marker of coronary artery disease in a Brazilian sample
Provedor de dados: 56
Autores: Scartezini,M.; Zago,M.A.; Chautard-Freire-Maia,E.A.; Pazin-Filho,A.; Marin-Neto,J.A.; Hotta,J.K.S.; Nascimento,A.J.; Dos-Santos,J.E.
Studies that consider polymorphisms within the apolipoprotein B (apo B) gene as risk factors for coronary artery disease (CAD) have reported conflicting results. The aim of the present study was to search for associations between two DNA RFLPs (XbaI and EcoRI) of the apo B gene and CAD diagnosed by angiography. In the present study we compared 116 Brazilian patients (92 men) with CAD (CAD+) to 78 control patients (26 men) without ischemia or arterial damage (CAD-). The allele frequencies at the XbaI (X) and EcoRI (E) sites did not differ between groups. The genotype distributions of CAD+ and CAD- patients were different (chi²(1) = 6.27, P = 0.012) when assigned to two classes (X-X-/E+E+ and the remaining XbaI/EcoRI genotypes). Multivariate logistic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apo B XbaI/EcoRI polymorphisms; Coronary artery disease; CAD risk.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300012

Effect of meconium ileus on the clinical prognosis of patients with cystic fibrosis
Provedor de dados: 56
Autores: Oliveira,M.C.L.A.; Reis,F.J.C.; Monteiro,A.P.A.F.; Penna,F.J.
The objective of the present study was to determine the possible prognostic factors which may explain the difference in the survival of patients with cystic fibrosis (CF) with and without meconium ileus. Over a period of 20 years, 127 patients with CF, whose diagnosis was confirmed by typical clinical characteristics and altered sweat chloride levels, were studied retrospectively. The patients were divided into two groups: group 1 consisted of patients who presented CF and meconium ileus (N = 9), and group 2 consisted of patients with CF without meconium ileus (N = 118). The characteristics studied were based on data obtained upon admission of the patients using a specific protocol. Demographic, clinical, nutritional and laboratory data were obtained. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cystic fibrosis; Meconium ileus; Survival; Nutrition; Genotype.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100005

Antipyretic and antioxidant activities of 5-trifluoromethyl-4,5-dihydro-1H-pyrazoles in rats
Provedor de dados: 56
Autores: Pasin,J.S.M.; Ferreira,A.P.O.; Saraiva,A.L.L.; Ratzlaff,V.; Andrighetto,R.; Machado,P.; Marchesan,S.; Zanette,R.A.; Bonacorso,H.G.; Zanatta,N.; Martins,M.A.P.; Ferreira,J.; Mello,C.F.
The objective of this study was to determine the effect of eight 5-hydroxy-5-trifluoromethyl-4,5-dihydro-1H-1-carboxyamidepyrazoles (TFDPs) on rat body temperature and baker’s yeast-induced fever. TFDPs or vehicle (5% Tween 80 in 0.9% NaCl, 5 mL/kg) were injected subcutaneously and rectal temperature was measured as a function of time in 28-day-old male Wistar rats (N = 5-12 per group). Antipyretic activity was determined in feverish animals injected with baker’s yeast (Saccharomyces cerevisiae suspension, 0.135 mg/kg, 10 mL/kg, ip). 3-Ethyl- and 3-propyl-TFDP (140 and 200 μmol/kg, respectively, 4 h after yeast injection) attenuated baker’s yeast-induced fever by 61 and 82%, respectively. These two effective antipyretics were selected for subsequent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyrazole derivatives; Fever; Cytokine levels; Cyclooxygenase.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200009

Diagnostic investigation of ventilator-associated pneumonia using bronchoalveolar lavage: comparative study with a postmortem lung biopsy
Provedor de dados: 56
Autores: Balthazar,A.B.; Von Nowakonski,A.; De Capitani,E.M.; Bottini,P.V.; Terzi,R.G.G.; Araújo,S.
The purpose of the present study was to validate the quantitative culture and cellularity of bronchoalveolar lavage (BAL) for the diagnosis of ventilator-associated pneumonia (VAP). A prospective validation test trial was carried out between 1992 and 1997 in a general adult intensive care unit of a teaching hospital. Thirty-seven patients on mechanical ventilation with suspected VAP who died at most three days after a BAL diagnostic procedure were submitted to a postmortem lung biopsy. BAL effluent was submitted to Gram staining, quantitative culture and cellularity count. Postmortem lung tissue quantitative culture and histopathological findings were considered to be the gold standard exams for VAP diagnosis. According to these criteria, 20 patients (54%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nosocomial pneumonia; Mechanical ventilation; Diagnostic techniques; Bronchoalveolar lavage; Lung biopsy; Ventilator-associated pneumonia.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800004

Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus
Provedor de dados: 56
Autores: Ruiz,F.S.; Andersen,M.L.; Zager,A.; Martins,R.C.S.; Tufik,S.
The objective of the present study was to determine whether sleep deprivation (SD) would promote changes in lymphocyte numbers in a type 1 diabetes model (non-obese diabetic, NOD, mouse strain) and to determine whether SD would affect female and male NOD compared to Swiss mice. The number of lymphocytes in peripheral blood after 24 and 96 h of SD (by multiple platform method) or equivalent period of time in home-cage controls was examined prior to the onset of diabetes. SD for 96 h significantly reduced lymphocytes in male Swiss mice compared to control (8.6 ± 2.1 vs 4.1 ± 0.7 10³/µL; P &lt; 0.02). In male NOD animals, 24- and 96-h SD caused a significant decrease of lymphocytes compared to control (4.4 ± 0.3 vs 1.6 ± 0.5; P &lt; 0.001 and 4.4 ± 0.3 vs 0.9...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sleep deprivation; Stress; Lymphopenia; Diabetes; Non-obese diabetic mouse; Swiss mice.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500005

Effects of ouabain on vascular reactivity
Provedor de dados: 56
Ouabain is an endogenous substance occurring in the plasma in the nanomolar range, that has been proposed to increase vascular resistance and induce hypertension. This substance acts on the&lt;FONT FACE="Symbol"&gt; a&lt;/font&gt;-subunit of Na+,K+-ATPase inhibiting the Na+-pump activity. In the vascular smooth muscle this effect leads to intracellular Na+ accumulation that reduces the activity of the Na+/Ca2+ exchanger and to an increased vascular tone. It was also suggested that circulating ouabain, even in the nanomolar range, sensitizes the vascular smooth muscle to vasopressor substances. We tested the latter hypothesis by studying the effects of ouabain in the micromolar and nanomolar range on phenylephrine (PE)-evoked pressor responses. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ouabain; Na+-pump; Hypertension; Rat tail vascular bed perfusion; Arterial blood pressure; Vascular reactivity; Phenylephrine.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400016

Effects on prolactin secretion and binding to dopaminergic receptors in sleep-deprived lupus-prone mice
Provedor de dados: 56
Autores: Palma,B.D.; Hipolide,D.C.; Tufik,S.
Sleep disturbances have far-reaching effects on the neuroendocrine and immune systems and may be linked to disease manifestation. Sleep deprivation can accelerate the onset of lupus in NZB/NZWF1 mice, an animal model of severe systemic lupus erythematosus. High prolactin (PRL) concentrations are involved in the pathogenesis of systemic lupus erythematosus in human beings, as well as in NZB/NZWF1 mice. We hypothesized that PRL could be involved in the earlier onset of the disease in sleep-deprived NZB/NZWF1 mice. We also investigated its binding to dopaminergic receptors, since PRL secretion is mainly controlled by dopamine. Female NZB/NZWF1 mice aged 9 weeks were deprived of sleep using the multiple platform method. Blood samples were taken for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Systemic lupus erythematosus; NZB/NZWF1 mice; Prolactin; Stress; Autoradiography; Dopamine transporter.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300012

Localization of NMDA receptors in the cerebral cortex: a schematic overview
Provedor de dados: 56
The fundamental role of N-methyl-D-aspartate (NMDA) receptors in many cortical functions has been firmly defined, as has its involvement in a number of neurological and psychiatric diseases. However, until recently very little was known about the anatomical localization of NMDA receptors in the cerebral cortex of mammals. The recent application of molecular biological techniques to the study of NMDA receptors has provided specific tools which have greatly expanded our understanding of the localization of NMDA receptors in the cerebral cortex. In particular, immunocytochemical studies on the distribution of cortical NMDA receptors have shown that NMDA receptors are preferentially localized on dendritic spines, have disclosed an unknown fraction of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glutamate; Ionotropic receptors; Autoreceptors; Heteroreceptors; Astrocytes; Neuron-glia signaling.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500001

Are the current chronic allograft nephropathy grading systems sufficient to predict renal allograft survival?
Provedor de dados: 56
Autores: Moscoso-Solorzano,G.T.; Mastroianni-Kirsztajn,G.; Ozaki,K.S.; Araujo,S.; Franco,M.F.; Pacheco-Silva,A.; Camara,N.O.S.
A major problem in renal transplantation is identifying a grading system that can predict long-term graft survival. The present study determined the extent to which the two existing grading systems (Banff 97 and chronic allograft damage index, CADI) correlate with each other and with graft loss. A total of 161 transplant patient biopsies with chronic allograft nephropathy (CAN) were studied. The samples were coded and evaluated blindly by two pathologists using the two grading systems. Logistic regression analyses were used to evaluate the best predictor index for renal allograft loss. Patients with higher Banff 97 and CADI scores had higher rates of graft loss. Moreover, these measures also correlated with worse renal function and higher proteinuria...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin angiotensin-converting enzyme inhibitor; Chronic allograft damage index; Banff 97; Renal allograft survival; Kidney transplantation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000011

Moderate-term reproducibility of heart rate variability during rest and light to moderate exercise in children
Provedor de dados: 56
Autores: Leicht,A.S.; Allen,G.D.
Previous studies have demonstrated the high reproducibility of heart rate variability (HRV) measures in adults while little information exists concerning HRV reproducibility in children. Subsequently, the aim of the current study was to examine the moderate-term reproducibility of heart rate and frequency domain measures of HRV during rest and light to moderate exercise in children. Ten healthy children (6 males, 4 females) aged between 7 and 12 years of age volunteered for this study with HRV recordings obtained during supine rest and three treadmill walking exercise work rates (≤60% maximum heart rate), initially and then 8 weeks later. Differences (P &lt; 0.05) between variables were examined using paired t-tests or Wilcoxon signed rank tests while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autonomic nervous system; Heart rate; Parasympathetic; Neural control; Reliability.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700013

Apolipoprotein CIII polymorphism and triglyceride levels of a Japanese population living in Southern Brazil
Provedor de dados: 56
Autores: Parzianello,L.; Oliveira,G.; Coelho,J.C.
Apolipoprotein CIII (apo-CIII) participates in the regulation of triglyceride-rich lipoprotein metabolism. Several polymorphic sites have been detected within and around the apo-CIII gene. Here, we examined the relationship between apo-CIII SstI polymorphism (CC, CG, GG genotypes) and plasma triglyceride (TG) levels in a group of 159 Japanese individuals living in Southern Brazil. The sample was divided into a group of Japanese descendants (N = 51) with high TG (HTG; &gt;200 mg/dL) and a group of Japanese descendants (N = 108) with normal TG (NTG; &lt;200 mg/dL). TG and total cholesterol levels were analyzed by an enzymatic method using the Labtest-Diagnostic kit and high- and low-density lipoproteins by a direct method using the Labtest-Diagnostic kit and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triglycerides; SstI polymorphism; Apolipoprotein CIII; Japanese population.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600004

Measurement of intestinal permeability using mannitol and lactulose in children with diarrheal diseases
Provedor de dados: 56
Autores: Barboza Jr.,M.S.; Silva,T.M.J.; Guerrant,R.L.; Lima,A.A.M.
The excretion ratio of lactulose/mannitol in urine has been used to assess the extension of malabsorption and impairment of intestinal permeability. The recovery of lactulose and mannitol in urine was employed to evaluate intestinal permeability in children with and without diarrhea. Lactulose and mannitol probes were measured using high-performance liquid chromatography with pulsed amperometric detection (HPLC-PAD). Two groups of solutions containing 60 µM sugars were prepared. Group I consisted of glucosamine, mannitol, melibiose and lactulose, and group II of inositol, sorbitol, glucose and lactose. In the study of intra-experiment variation, a sample of 50 µl from each group was submitted to 4 successive determinations. The recovered amounts and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Permeability test; Mannitol; Lactulose; HPLC-PAD; Diarrhea.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200008

Linderae radix ethanol extract attenuates alcoholic liver injury via attenuating inflammation and regulating gut microbiota in rats
Provedor de dados: 56
Autores: Lou,Zhaohuan; Wang,Junwei; Chen,Yingjun; Xu,Chandi; Chen,Xinyi; Shao,Tiejuan; Zhang,Kena; Pan,Hongying.
This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway. ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days. Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/low dose groups (ALD rats fed with 4, 2, or 1 g LREE/kg). NF-κB and LPS levels were evaluated. Liver pathological changes and intestinal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lindera aggregata (Sims) Kosterm; Ethanol extract; Alcoholic liver disease; Intestinal endotoxemia; 16S ribosomal RNA gene sequencing; Tight junction protein.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600601

Inactivated Parapoxvirus ovis induces a transient increase in the expression of proinflammatory, Th1-related, and autoregulatory cytokines in mice
Provedor de dados: 56
Autores: Anziliero,D.; Weiblen,R.; Kreutz,L.C.; Spilki,F.; Flores,E.F.
The immunostimulatory properties of inactivated Parapoxvirus ovis (iPPVO) have long been investigated in different animal species and experimental settings. In this study, we investigated the effects of iPPVO on cytokine expression in mice after intraperitoneal inoculation. Spleen and sera collected from iPPVO-treated mice at intervals after inoculation were submitted to cytokine mRNA determination by real-time PCR (qPCR), serum protein concentration by ELISA, and interferon (IFN)-α/β activity by bioassay. The spleen of iPPVO-treated animals showed a significant increase in mRNA expression of all cytokines assayed, with different kinetics and magnitude. Proinflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-alpha (TNF-α), and IL-8 mRNA peaked...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokine; MRNA; ORF virus; Immunostimulant; Innate immune response.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200110

Plasma metanephrines: a novel and cost-effective test for pheochromocytoma
Provedor de dados: 56
Autores: Eisenhofer,G.; Walther,M.; Keiser,H.R.; Lenders,J.W.M.; Friberg,P.; Pacak,K.
Pheochromocytomas are rare chromaffin cell tumors that nevertheless must be excluded in large numbers of patients who develop sustained or episodic hypertension as well as in many others with suggestive symptoms or with a familial history of pheochromocytoma. Diagnosis of pheochromocytoma depends importantly on biochemical evidence of excess catecholamine production by a tumor. Imperfect sensitivity and specificity of commonly available biochemical tests and the low incidence of the tumor among the tested population mean that considerable time and effort can be expended in confirming or ruling out pheochromocytoma in patients where the tumor is suspected. Measurements of plasma free metanephrines provide a superior test compared to other available tests...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pheochromocytoma; Hypertension; Sympathetic nervous system; Adrenal gland; Metanephrines; Catecholamines.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000005

Method for automatic detection of wheezing in lung sounds
Provedor de dados: 56
Autores: Riella,R.J.; Nohama,P.; Maia,J.M.
The present report describes the development of a technique for automatic wheezing recognition in digitally recorded lung sounds. This method is based on the extraction and processing of spectral information from the respiratory cycle and the use of these data for user feedback and automatic recognition. The respiratory cycle is first pre-processed, in order to normalize its spectral information, and its spectrogram is then computed. After this procedure, the spectrogram image is processed by a two-dimensional convolution filter and a half-threshold in order to increase the contrast and isolate its highest amplitude components, respectively. Thus, in order to generate more compressed data to automatic recognition, the spectral projection from the processed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheezes; Lung sounds; Spectrogram; Digital image processing; Artificial neural networks.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700013

Prazosin blocks the glutamatergic effects of N-methyl-D-aspartic acid on lordosis behavior and luteinizing hormone secretion in the estrogen-primed female rat
Provedor de dados: 56
Autores: Landa,A.I.; Cabrera,R.J.; Gargiulo,P.A.
We have observed that intracerebroventricular (icv) injection of selective N-methyl-D-aspartic acid (NMDA)-type glutamatergic receptor antagonists inhibits lordosis in ovariectomized (OVX), estrogen-primed rats receiving progesterone or luteinizing hormone-releasing hormone (LHRH). When NMDA was injected into OVX estrogen-primed rats, it induced a significant increase in lordosis. The interaction between LHRH and glutamate was previously explored by us and another groups. The noradrenergic systems have a functional role in the regulation of LHRH release. The purpose of the present study was to explore the interaction between glutamatergic and noradrenergic transmission. The action of prazosin, an alpha1- and alpha2b-noradrenergic antagonist, was studied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sexual behavior; Glutamate; Luteinizing hormone; Noradrenergic transmission; Prazosin; N-methyl-D-aspartic acid.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300007

GLUT4 protein expression in obese and lean 12-month-old rats: insights from different types of data analysis
Provedor de dados: 56
Autores: Seraphim,P.M.; Nunes,M.T.; Machado,U.F.
GLUT4 protein expression in white adipose tissue (WAT) and skeletal muscle (SM) was investigated in 2-month-old, 12-month-old spontaneously obese or 12-month-old calorie-restricted lean Wistar rats, by considering different parameters of analysis, such as tissue and body weight, and total protein yield of the tissue. In WAT, a ~70% decrease was observed in plasma membrane and microsomal GLUT4 protein, expressed as µg protein or g tissue, in both 12-month-old obese and 12-month-old lean rats compared to 2-month-old rats. However, when plasma membrane and microsomal GLUT4 tissue contents were expressed as g body weight, they were the same. In SM, GLUT4 protein content, expressed as µg protein, was similar in 2-month-old and 12-month-old obese rats, whereas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin resistance; Obesity; Weight loss; GLUT4 protein.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000018

Association among genetic predisposition, gut microbiota, and host immune response in the etiopathogenesis of inflammatory bowel disease
Provedor de dados: 56
Autores: Basso,P.J.; Fonseca,M.T.C.; Bonfá,G.; Alves,V.B.F.; Sales-Campos,H.; Nardini,V.; Cardoso,C.R.B.
Inflammatory bowel disease (IBD), which includes Crohn's disease (CD) and ulcerative colitis (UC), is a chronic disorder that affects thousands of people around the world. These diseases are characterized by exacerbated uncontrolled intestinal inflammation that leads to poor quality of life in affected patients. Although the exact cause of IBD still remains unknown, compelling evidence suggests that the interplay among immune deregulation, environmental factors, and genetic polymorphisms contributes to the multifactorial nature of the disease. Therefore, in this review we present classical and novel findings regarding IBD etiopathogenesis. Considering the genetic causes of the diseases, alterations in about 100 genes or allelic variants, most of them in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammatory bowel disease; Crohn's disease; Ulcerative colitis; Immune response; Etiology; Microbiota.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900727

Stimulated mast cells promote maturation of myocardial microvascular endothelial cell neovessels by modulating the angiopoietin-Tie-2 signaling pathway
Provedor de dados: 56
Autores: Wang,Z.H.; Zhu,W.; Tao,J.P.; Zhang,Q.Y.; Wei,M.
Angiopoietin (Ang)-1 and Ang-2 interact in angiogenesis to activate the Tie-2 receptor, which may be involved in new vessel maturation and regression. Mast cells (MCs) are also involved in formation of new blood vessels and angiogenesis. The present study was designed to test whether MCs can mediate angiogenesis in myocardial microvascular endothelial cells (MMVECs). Using a rat MMVEC and MC co-culture system, we observed that Ang-1 protein levels were very low even though its mRNA levels were increased by MCs. Interestingly, MCs were able to enhance migration, proliferation, and capillary-like tube formation, which were associated with suppressed Ang-2 protein expression, but not Tie-2 expression levels. These MCs induced effects that could be reversed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial microvascular endothelial cells; Mast cell; Angiogenesis; Angiopoietin; Tie-2.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100920

Galactose oxidation using 13C in healthy and galactosemic children
Provedor de dados: 56
Autores: Resende-Campanholi,D.R.; Porta,G.; Ferrioli,E.; Pfrimer,K.; Ciampo,L.A. Del; Junior,J.S. Camelo.
Galactosemia is an inborn error of galactose metabolism that occurs mainly as the outcome of galactose-1-phosphate uridyltransferase (GALT) deficiency. The ability to assess galactose oxidation following administration of a galactose-labeled isotope (1-13C-galactose) allows the determination of galactose metabolism in a practical manner. We aimed to assess the level of galactose oxidation in both healthy and galactosemic Brazilian children. Twenty-one healthy children and seven children with galactosemia ranging from 1 to 7 years of age were studied. A breath test was used to quantitate 13CO2 enrichment in exhaled air before and at 30, 60, and 120 min after the oral administration of 7 mg/kg of an aqueous solution of 1-13C-galactose to all children. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galactosemias; Isotope labeling; Children; Breath tests.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300280

A routine electrocardiogram cannot be used to determine the size of myocardial infarction in the rat
Provedor de dados: 56
Autores: Bonilha,A.M.M.; Saraiva,R.M.; Kanashiro,R.M.; Portes,L.A.; Antonio,E.L.; Tucci,P.J.F.
Nine lead electrocardiograms of non-infarcted (N = 61) and infarcted (N = 71) female Wistar rats (200-250 g) were analyzed in order to distinguish left ventricle myocardial infarction (MI) larger than 40% (LMI) from MI smaller than 40% (SMI). MI larger than 40% clearly caused a deviation of ÂQRS and ÂT from normal values of 270-360 degrees to 90-270 degrees. Infarcted rats showed Q wave in D1 larger than 1 mm with 94% sensitivity and 100% specificity. The sum of QRS positivity in V1, V2 and V6 lower than 10 mm identified MI with 82% sensitivity and 100% specificity. The data showed that MI can be easily and reliably diagnosed by electrocardiogram in the rat. However, contradicting what is frequently believed, when specificity and sensitivity were analyzed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial infarction size; Rats; Electrocardiogram; Echocardiogram; ROC curve.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400016

CYP2A6 and CYP2E1 polymorphisms in a Brazilian population living in Rio de Janeiro
Provedor de dados: 56
Autores: Rossini,A.; Soares Lima,S.; Rapozo,D.C.M.; Faria,M.; Albano,R.M.; Ribeiro Pinto,L.F.
Cytochrome P450 (CYP) is a superfamily of enzymes involved in the metabolism of endogenous compounds and xenobiotics. CYP2A6 catalyzes the oxidation of nicotine and the activation of carcinogens such as aflatoxin B1 and nitrosamines. CYP2E1 metabolizes ethanol and other low-molecular weight compounds and can also activate nitrosamines. The CYP2A6 and CYP2E1 genes are polymorphic, altering their catalytic activities and susceptibility to cancer and other diseases. A number of polymorphisms described are ethnic-dependent. In the present study, we determined the genotype and allele frequencies of the main CYP2A6 and CYP2E1 polymorphisms in a group of 289 volunteers recruited at the Central Laboratory of Hospital Universitário Pedro Ernesto. They had been...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CYP2A6; CYP2E1; Polymorphism; Brazil; Ethnic groups.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200005

Clinical outcome of protein-energy malnourished patients in a Brazilian university hospital
Provedor de dados: 56
Autores: Pasquini,T.A.S.; Neder,H.D.; Araújo-Junqueira,L.; De-Souza,D.A.
Protein-energy malnutrition (PEM) is a treatable disease with high prevalence among hospitalized patients. It can cause significant increases in the duration of hospitalization and costs. PEM is especially important for health systems since malnourished patients present higher morbidity and mortality. The objective of the present study was to assess the evolution of nutritional status (NS) and the effect of malnutrition on clinical outcome of patients at a public university hospital of high complexity in Brazil. Patients hospitalized in internal medicine (n = 54), oncology (n = 43), and infectious diseases (n = 12) wards were included. NS was evaluated using subjective global assessment up to 48 h after admission, and thereafter at intervals of 4-6 days....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein-energy malnutrition; Hospital malnutrition; Assessment of nutritional status; Evolution of nutritional status; Nutritional therapy; Subjective global assessment.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200028

Prostate-specific membrane antigen can promote in vivo osseous metastasis of prostate cancer cells in mice
Provedor de dados: 56
Autores: Zhao,Liang-Yun; Mao,Xiao-Peng; Chao,Kai-Yuan; Guo,Sheng-Jie; Qiu,Shao-Peng.
Reports remain insufficient on whether and how prostate-specific membrane antigen (PSMA) can influence in vivo osseous metastasis of prostate cancer (PCa). In the present study, the authors induced stable expression of PSMA in mouse PCa cell line RM-1. In vivo osseous metastasis was induced in 37 6-week-old female C57BL/6 mice weighing 22.45 ± 0.456 g. RM-1 cells were actively injected into the femoral bone cavity, leading to bilateral dissymmetry of bone density in the femoral bone. Tumor cells were also detected in bone tissue by pathological examination. The impact on bone density was demonstrated by the significant difference between animals injected with RM-PSMA cells (0.0738 ± 0.0185 g/cm²) and animals injected with RM-empty plasmid cells (0.0895 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate carcinoma; Prostate-specific membrane antigen; In vitro; Osseous metastasis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800008

T cell receptor excision circles as a tool for evaluating thymic function in young children
Provedor de dados: 56
Autores: Levy,A.; Rangel-Santos,A.; Torres,L.C.; Silveira-Abreu,G.; Agena,F.; Carneiro-Sampaio,M.
The thymus is a primary lymphoid organ responsible for the maturation of T cells as well as the immunological central tolerance. It is in the antenatal period and infancy that it plays its major role. In clinical practice, T cell receptor excision circles (TRECs) are considered a direct and reliable measure of the thymic function. TRECs are a by-product of DNA formation in gene rearrangement of T cell receptors. They are stable and they do not duplicate during mitosis, representing the recent emigrant T cells from the thymus. Despite their importance, TRECs have been neglected by physicians and there is a lack of data regarding thymic function during infancy of healthy children. In order to evaluate thymic function in the first years of life, we propose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human thymus; TRECs; Thymic function; Infants.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700601

Promoting inflammatory lymphangiogenesis by vascular endothelial growth factor-C (VEGF-C) aggravated intestinal inflammation in mice with experimental acute colitis
Provedor de dados: 56
Autores: Wang,X.L.; Zhao,J.; Qin,L.; Qiao,M.
Angiogenesis and lymphangiogenesis are thought to play a role in the pathogenesis of inflammatory bowel diseases (IBD). However, it is not understood if inflammatory lymphangiogenesis is a pathological consequence or a productive attempt to resolve the inflammation. This study investigated the effect of lymphangiogenesis on intestinal inflammation by overexpressing a lymphangiogenesis factor, vascular endothelial growth factor-C (VEGF-C), in a mouse model of acute colitis. Forty eight-week-old female C57BL/6 mice were treated with recombinant adenovirus overexpressing VEGF-C or with recombinant VEGF-C156S protein. Acute colitis was then established by exposing the mice to 5% dextran sodium sulfate (DSS) for 7 days. Mice were evaluated for disease activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Lymphangiogenesis; Microvessel density; Acute colitis; VEGF-C.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500602

Comparison of serum PCT and CRP levels in patients infected by different pathogenic microorganisms: a systematic review and meta-analysis
Provedor de dados: 56
Autores: Tang,Jun-Hua; Gao,Dong-Ping; Zou,Peng-Fei.
To avoid the abuse and misuse of antibiotics, procalcitonin (PCT) and C-reactive protein (CRP) have been used as new approaches to identify different types of infection. Multiple databases were adopted to search relevant studies, and the articles that satisfied the inclusion criteria were included. Meta-analyses were conducted with Review Manager 5.0, and to estimate the quality of each article, risk of bias was assessed. Eight articles satisfied the inclusion criteria. The concentrations of both PCT and CRP in patients with bacterial infection were higher than those with non-bacterial infection. Both PCT and CRP levels in patients with G− bacterial infection were higher than in those with G+ bacterial infection and fungus infection. In the G+ bacterial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulmonary infection; PCT; CRP; Bacteria; Fungus.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700610

Is a contrast study really necessary prior to ureteroscopy?
Provedor de dados: 56
Autores: Bayrak,O.; Demirbas,A.; Doluoglu,O.G.; Karakan,T.; Resorlu,B.; Kardas,S.; Tepeler,A.; Tangal,S.; Adanur,S.; Celik,O.
This study aimed to evaluate the effect of preoperative imaging techniques on the success and complication rates of ureteroscopy. We performed a retrospective analysis of 736 patients (455 males and 281 females), with a mean age of 45.5±15.2 years (range, 1-88 years), who underwent rigid ureteroscopic procedures for removal of ureteral stones. Patients were divided into 4 groups according to the type of imaging modality used: group I, intravenous urography (n=116); group II, computed tomography (n=381); group III, computed tomography and intravenous urography (n=91), and group IV, ultrasonography and abdominal plain film (n=148). Patients’ demographics, stone size and location, prior shock wave lithotripsy, lithotripsy technique, operation time, success...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Computed tomography; Imaging; Intravenous urography; Stone; Ureteroscopy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100703

Functional evidence that the central renin-angiotensin system plays a role in the pressor response induced by central injection of carbachol
Provedor de dados: 56
We investigated the effects of losartan, an AT1-receptor blocker, and ramipril, a converting enzyme inhibitor, on the pressor response induced by angiotensin II (ANG II) and carbachol (a cholinergic receptor agonist). Male Holtzman rats (250-300 g) with a stainless steel cannula implanted into the lateral ventricle (LV) were used. The injection of losartan (50 nmol/1 &amp;amp;micro;l) into the LV blocked the pressor response induced by ANG II (12 ng/1 &amp;amp;micro;l) and carbachol (2 nmol/1 &amp;amp;micro;l). After injection of ANG II and carbachol into the LV, mean arterial pressure (MAP) increased to 31 &amp;amp;plusmn; 1 and 28 &amp;amp;plusmn; 2 mmHg, respectively. Previous injection of losartan abolished the increase in MAP induced by ANG II and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pressor response; Central angiotensin; Carbachol.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400010

Short duration of neutralizing antibody titers after pre-exposure rabies vaccination with suckling mouse brain vaccine
Provedor de dados: 56
Autores: Zanetti,C.R.; Consales,C.A.; Rodrigues-da-Silva,A.C.; Toyoshima,Y.K.; Pereira,O.A.C.
The human anti-rabies pre-exposure treatment currently used in Brazil, employing a 1-ml dose of suckling mouse brain vaccine (SMBV) administered on days 0, 2, 4 and 28, was compared to an alternative treatment with two 1 ml-doses on day 0, and one 1 ml-dose injected on days 7 and 21. The latter induced higher virus-neutralizing antibody (VNA) titers on day 21. Both Brazilian rabies vaccines produced with PV or CVS rabies virus strains were tested. Two additional volunteer vaccinee groups, receiving the pre-exposure and the abbreviated post-exposure schedules recommended by the WHO using cell-culture vaccine (CCV) produced with PM rabies virus strain, were included as reference. The VNA were measured against both PV and CVS strains on days 21, 42 and 180 by...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rabies; Rabies vaccine; Pre-exposure; Suckling mouse brain vaccine.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000007

lncRNA MEG3 inhibits the growth of hepatocellular carcinoma cells by sponging miR-9-5p to upregulate SOX11
Provedor de dados: 56
Autores: Liu,Zhi; Chen,Jian Yu; Zhong,Yang; Xie,Liang; Li,Jian Shui.
The long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3), a tumor suppressor, is critical for the carcinogenesis and progression of different cancers, including hepatocellular carcinoma (HCC). To date, the roles of lncRNA MEG3 in HCC are not well illustrated. Therefore, this study used western blot and qRT-PCR to evaluate the expression of MEG3, miR-9-5p, and Sex determining Region Y-related HMG-box 11 (SOX11) in HCC tissues and cell lines. RNA pull-down and luciferase reporter assay were used to evaluate these molecular interactions. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry detected the viability and apoptosis of HCC cells, respectively. The results showed that MEG3 and SOX11 were poorly expressed but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma MEG3; MiR-9-5p SOX11 lncRNA.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000604

Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids
Provedor de dados: 56
Autores: Azevedo,R.B.; Lacava,Z.G.M.; Miyasaka,C.K.; Chaves,S.B.; Curi,R.
Human and animal immune functions present sex dimorphism that seems to be mainly regulated by sex hormones. In the present study, the activities of the antioxidant enzymes total superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were measured in intraperitoneal resident macrophages from adult male and female rats. In addition to comparing males and females, we also examined the regulation of these enzyme activities in macrophages by sex steroids. GSH-Px activity did not differ between male and female macrophages. However, both total SOD and CAT activities were markedly higher in females than in males (83 and 180%). Removal of the gonads in both males and females (comparison between castrated groups) increased the difference in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Antioxidant enzymes; Macrophages; Sex hormones; Estrogen; Progesterone; Castration.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500018

Primary mechanism of apoptosis induction in a leukemia cell line by fraction FA-2-b-ß prepared from the mushroom Agaricus blazei Murill
Provedor de dados: 56
Autores: Gao,L.; Sun,Y.; Chen,C.; Xi,Y.; Wang,J.; Wang,Z.
Agaricus blazei Murill is a native Brazilian mushroom which functions primarily as an anticancer substance in transplanted mouse tumors. However, the mechanism underlying this function of A. blazei Murill remains obscure. The present study was carried out to investigate the effect of fraction FA-2-b-ß, an RNA-protein complex isolated from A. blazei Murill, on human leukemia HL-60 cells in vitro. Typical apoptotic characteristics were determined by morphological methods using DNA agarose gel electrophoresis and flow cytometry. The growth suppressive effect of fraction FA-2-b-ß on HL-60 cells in vitro occurred in a dose- (5-80 µg/mL) and time-dependent (24-96 h) manner. The proliferation of HL-60 cells (1 x 10(5) cells/mL) treated with 40 µg/mL of fraction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus blazei Murill; Fraction FA-2-b-ß; Apoptosis; Caspase-3; Telomerase; HL-60 cells.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100015

Effects of caffeine on time to exhaustion in exercise performed below and above the anaerobic threshold
Provedor de dados: 56
Autores: Denadai,B.S.; Denadai,M.L.D.R.
Controversy still exists concerning the potential ergogenic benefit of caffeine (CAF) for exercise performance. The purpose of this study was to compare the effects of CAF ingestion on endurance performance during exercise on a bicycle ergometer at two different intensities, i.e., approximately 10% below and 10% above the anaerobic threshold (AT). Eight untrained males, non-regular consumers of CAF, participated in this study. AT, defined as the intensity (watts) corresponding to a lactate concentration of 4 mM, was determined during an incremental exercise test from rest to exhaustion on an electrically braked cycle ergometer. On the basis of these measurements, the subjects were asked to cycle until exhaustion at two different intensities, i.e.,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Caffeine; Anaerobic threshold; Endurance; Lactate; Perceived exertion.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400017

Diagnostic profile of inpatients as a determinant of length of stay in a general hospital psychiatric unit
Provedor de dados: 56
Autores: Hallak,J.E.C.; Crippa,J.A.S.; Vansan,G.; Zuardi,A.W.
The aim of this study was to determine if the diagnostic profile of inpatients of a psychiatric unit in a general hospital influences the length of stay. The results of a retrospective survey comprising the first 16 years of operation of the Psychiatric Unit of the Ribeirão Preto General Hospital (PURP) showed that the progressive increase observed in the length of stay correlated with the increase in percentage of schizophrenia diagnosis, after the 8th year of hospital operation, and of affective disorders, after the 12th year. The length of hospitalization kept increasing until the 16th year, even though there was no change in the diagnostic profile of the patients admitted to the unit. In a prospective study encompassing the next six months, 61...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psychiatric unit; General hospital; Diagnostic category; Length of stay.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900014

Distribution of the human leukocyte antigen class II alleles in Brazilian patients with chronic hepatitis C virus infection
Provedor de dados: 56
Autores: Corghi,D.B.; Gonçales,N.S.L.; Marques,S.B.D.; Gonçales Jr.,F.L.
Hepatitis C virus (HCV) infection is a global medical problem. The current standard of treatment consists of the combination of peginterferon plus ribavirin. This regimen eradicates HCV in 55% of cases. The immune response to HCV is an important determinant of disease evolution and can be influenced by various host factors. HLA class II may play an important role in immune response against HCV. The objective of the present study was to determine the distribution of HLA class II (DRB1 and DQB1) alleles, their association with chronic HCV infection and their response to interferon therapy. One hundred and two unrelated white Brazilian patients with chronic HCV infection, 52 responders (45 males and 7 females) and 50 non-responders (43 males and 7 females) to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HLA class II; HLA DR/DQ; HLA genotyping PCR; Hepatitis C virus; Chronic hepatitis C; Interferon treatment.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000009

Effects of type of physical exercise and leisure activities on the depression scores of obese Brazilian adolescent girls
Provedor de dados: 56
Autores: Stella,S.G.; Vilar,A.P.; Lacroix,C.; Fisberg,M.; Santos,R.F.; Mello,M.T.; Tufik,S.
Several studies have indicated that depressive states may lead to hypokinesia with diminished metabolic rate and energy use. Hypokinesia associated with certain eating behaviors may lead to an unfavorable energy balance that can contribute to the emergence and prevalence of obesity among children and adults. The purpose of the present study was to examine the possibility of reducing depression inventory scores in female adolescents with third-degree obesity while testing the effectiveness of different exercise programs in reducing anxiety and depression scores. The sample consisted of 40 female subjects (mean age 16 ± 1.56 years) divided into 4 groups (aerobic training, anaerobic training, leisure activities, and control). Subjects had a body mass index of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adolescents; Physical exercise; Leisure activity; Obesity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100017

Ultra-short-term heart rate variability during resistance exercise in the elderly
Provedor de dados: 56
Autores: Arêas,G.P.T.; Caruso,F.C.R.; Simões,R.P.; Castello-Simões,V.; Jaenisch,R.B.; Sato,T.O.; Cabiddu,R.; Mendes,R.; Arena,R.; Borghi-Silva,A.
Despite the appeal of ultra-short-term heart rate variability (HRV) methods of analysis applied in the clinical and research settings, the number of studies that have investigated HRV by analyzing R-R interval (RRi) recordings shorter than 5 min is still limited. Moreover, ultra-short-term HRV analysis has not been extensively validated during exercise and, currently, no indications exist for its applicability during resistance exercise. The aim of the present study was to compare ultra-short-term HRV analysis with standard short-term HRV analysis during low-intensity, dynamic, lower limb resistance exercise in healthy elderly subjects. Heart rate (HR) and RRi signals were collected from 9 healthy elderly men during discontinuous incremental resistance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autonomic function; Older people; Leg press.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600614

The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells
Provedor de dados: 56
Autores: Fan,J.Z.; Yang,X.; Bi,Z.G.
We investigated whether 6-gingerol affects the maturation and proliferation of osteoblast-like MG63 cells in vitro. Osteoblast-like MG63 cells were treated with 6-gingerol under control conditions, and experimental inflammation was induced by tumor necrosis factor-α (TNF-α). Expression of different osteogenic markers and cytokines was analyzed by real-time PCR, Western blotting, and enzyme-linked immunosorbent assay. In addition, alkaline phosphatase (ALP) enzyme activity and biomineralization as markers for differentiation were measured. Treatment with 6-gingerol resulted in insignificant effects on the proliferation rate. 6-Gingerol induced the differentiation of osteoblast-like cells with increased transcription levels of osteogenic markers, upregulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 6-Gingerol; Interleukin-6; Nuclear factor-κB; Osteogenesis; Osteoblast.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700637

Diversity of genomic electropherotypes of naturally occurring equine herpesvirus 1 isolates in Argentina
Provedor de dados: 56
Autores: Galosi,C.M.; Norimine,J.; Echeverría,M.G.; Oliva,G.A.; Nosetto,E.O.; Etcheverrigaray,M.E.; Tohya,Y.; Mikami,T.
The genomes of 10 equine herpesvirus 1 (EHV-1) strains isolated in Argentina from 1979 to 1991, and a Japanese HH1 reference strain were compared by restriction endonuclease analysis. Two restriction enzymes, BamHI and BglII, were used and analysis of the electropherotypes did not show significant differences among isolates obtained from horses with different clinical signs. This suggests that the EHV-1 isolates studied, which circulated in Argentina for more than 10 years, belong to a single genotype.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Argentina; Electropherotypes; Equine herpesvirus.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600007

TMEM16 proteins: the long awaited calcium-activated chloride channels?
Provedor de dados: 56
Autores: Flores,C.A.; Cid,L.P.; Sepúlveda,F.V.; Niemeyer,M.I.
Currents mediated by calcium-activated chloride channels (CaCCs), observed for the first time in Xenopus oocytes, have been recorded in many cells and tissues ranging from different types of neurons to epithelial and muscle cells. CaCCs play a role in the regulation of excitability in neurons including sensory receptors. In addition, they are crucial mediators of chloride movements in epithelial cells where their activity regulates electrolyte and fluid transport. The roles of CaCCs, particularly in epithelia, are briefly reviewed with emphasis on their function in secretory epithelia. The recent identification by three independent groups, using different strategies, of TMEM16A as the molecular counterpart of the CaCC is discussed. TMEM16A is part of a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium-activated chloride channels; TMEM16; ANO; Intestinal secretion; Salivary gland; Lacrimal gland.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100001

The calcium-dependent protease of Loxosceles gaucho venom acts preferentially upon red cell band 3 transmembrane protein
Provedor de dados: 56
Autores: Barretto,O.C. de O.; Satake,M.; Nonoyama,K.; Cardoso,J.L.C.
Eighty micrograms red blood cell (RBC) ghosts from patients who had previously exhibited the cutaneous form of loxoscelism (presenting localized dermonecrosis) and the viscerocutaneous form of loxoscelism (presenting dermonecrosis, hemoglobinuria, hematuria, and jaundice) and from controls were incubated with 2.5 µg crude Loxosceles gaucho venom in 5 mM phosphate buffer, pH 7.4, at 37ºC. Among all membrane proteins, quantitative proteolysis of the important integral transmembrane protein 3 increased with venom dose and with incubation time from 30 to 120 min, as demonstrated by gel densitometry. Similar quantitative data were obtained for RBC ghosts from patients and from control subjects, a fact that argues against the possibility of genetic factors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loxoscelism; Loxosceles sp; Spider venom; Red blood cell membrane protein 3.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300004

Analysis of the application values of different combination schemes of liquid-based cytology and high-risk human papilloma virus test in the screening of high-grade cervical lesions
Provedor de dados: 56
Autores: Wang,Jian.
The aim of this study was to explore the value of different combination schemes of liquid-based cytology (LBC) and high-risk human papilloma virus (HPV) test in the screening of high-grade (≥CIN 2) cervical lesions. From 5727 women who had undergone examinations with LBC and high-risk HPV test, 1884 patients with positive results of either or both LBC and HPV test were included in this study and underwent cervical biopsy. Based on the pathological examination results, comparisons of the assessment indicators of all diagnostic tests were made, and the application values of LBC and high-risk HPV test and different combination schemes of the two in the screening of high-grade (≥CIN II) cervical lesions were estimated. Compared with the single test method, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liquid-based cytology; Human papilloma virus; Cervical cancer; ROC curve; High-grade.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100601

A biolistic process for in vitro gene transfer into chicken embryos
Provedor de dados: 56
Autores: Ribeiro,L.A.; Mariani,P.D.S.C.; Azevedo,J.L.; Rech,E.L.; Schmidt,G.S.; Coutinho,L.L.
Chicken embryos kept in culture medium were bombarded using a high helium gas pressure biolistic device. To optimize the factors that affect transformation efficiency, the lacZ gene under control of the human cytomegalovirus immediate early enhancer/promoter was used as a reporter gene. There was an inverse relationship between survival rate and transformation efficiency. The best conditions obtained for high embryo survival and high transformation efficiency were achieved with 800 psi helium gas pressure, 500 mmHg vacuum, gold particles, an 8 cm DNA-coated microparticle flying distance to the embryo and embryo placement 0.5 cm from the center of the particle dispersion cone. Under these conditions, transformation efficiency was 100%, survival rate 25% and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biolistic process; Chicken embryo; Gene transfer; SS-galactosidase; Green fluorescent protein; GFP.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900003

Heterogeneous response of adipose tissue to cancer cachexia
Provedor de dados: 56
Autores: Bertevello,P.S.; Seelaender,M.C.L.
Cancer cachexia causes disruption of lipid metabolism. Since it has been well established that the various adipose tissue depots demonstrate different responses to stimuli, we assessed the effect of cachexia on some biochemical and morphological parameters of adipocytes obtained from the mesenteric (MES), retroperitoneal (RPAT), and epididymal (EAT) adipose tissues of rats bearing Walker 256 carcinosarcoma, compared with controls. Relative weight and total fat content of tissues did not differ between tumor-bearing rats and controls, but fatty acid composition was modified by cachexia. Adipocyte dimensions were increased in MES and RPAT from tumor-bearing rats, but not in EAT, in relation to control. Ultrastructural alterations were observed in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer cachexia; Adipose tissue heterogeneity; Adipocyte heterogeneity.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900009

Mapping and signaling of neural pathways involved in the regulation of hydromineral homeostasis
Provedor de dados: 56
Autores: Antunes-Rodrigues,J.; Ruginsk,S.G.; Mecawi,A.S.; Margatho,L.O.; Cruz,J.C.; Vilhena-Franco,T.; Reis,W.L.; Ventura,R.R.; Reis,L.C.; Vivas,L.M.; Elias,L.L.K.
Several forebrain and brainstem neurochemical circuitries interact with peripheral neural and humoral signals to collaboratively maintain both the volume and osmolality of extracellular fluids. Although much progress has been made over the past decades in the understanding of complex mechanisms underlying neuroendocrine control of hydromineral homeostasis, several issues still remain to be clarified. The use of techniques such as molecular biology, neuronal tracing, electrophysiology, immunohistochemistry, and microinfusions has significantly improved our ability to identify neuronal phenotypes and their signals, including those related to neuron-glia interactions. Accordingly, neurons have been shown to produce and release a large number of chemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypothalamus; Gaseous neuromodulators; Neuropeptides; Endocannabinoids; Glial cells; Neurotransmitters.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000400327

Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies
Provedor de dados: 56
Autores: Verbisck,N.V.; Da-Silva,S.; Mortara,R.A.
We have raised monoclonal antibodies (mAbs) directed towards amastigote forms of Trypanosoma cruzi, and shown that mAbs 1D9 and 4B9 are carbohydrate while mAb 4B5 activity is resistant to periodate oxidation of the antigen. Here we used an ELISA to quantitate and compare the expression of surface epitopes on fixed parasites among different parasite isolates. The expression of markers varied among T. cruzi amastigotes isolated from infected cells or after extracellular differentiation of trypomastigotes. Moreover, we also observed an extensive polymorphic expression of these epitopes among amastigotes derived from different strains and clones. For instance, mAb 2C2 strongly and evenly reacted with 9 strains and clones (G, Y, CL, Tulahuen, MD, and F, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Amastigote; Monoclonal antibodies; Surface antigen; Polymorphism.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200011

Chronic excitotoxic lesion of the dorsal raphe nucleus induces sodium appetite
Provedor de dados: 56
Autores: Cavalcante-Lima,H.R.; Badauê-Passos Jr.,D.; de-Lucca Jr.,W.; Lima,H.R.C.; Costa-e-Sousa,R.H.; Olivares,E.L.; Cedraz-Mercez,P.L.; Reis,R.O.; Medeiros,M.A.; Côrtes,W.S.; Reis,L.C.
We determined if the dorsal raphe nucleus (DRN) exerts tonic control of basal and stimulated sodium and water intake. Male Wistar rats weighing 300-350 g were microinjected with phosphate buffer (PB-DRN, N = 11) or 1 µg/0.2 µl, in a single dose, ibotenic acid (IBO-DRN, N = 9 to 10) through a guide cannula into the DRN and were observed for 21 days in order to measure basal sodium appetite and water intake and in the following situations: furosemide-induced sodium depletion (20 mg/kg, sc, 24 h before the experiment) and a low dose of dietary captopril (1 mg/g chow). From the 6th day after ibotenic acid injection IBO-DRN rats showed an increase in sodium appetite (12.0 ± 2.3 to 22.3 ± 4.6 ml 0.3 M NaCl intake) whereas PB-DRN did not exceed 2 ml (P &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sodium appetite; Water intake; Dorsal raphe nucleus; Serotonergic system; Ibotenic acid.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100015

Fluorescent ligands for studying neuropeptide receptors by confocal microscopy
Provedor de dados: 56
Autores: Beaudet,A.; Nouel,D.; Stroh,T.; Vandenbulcke,F.; Dal-Farra,C.; Vincent,J.-P.
This paper reviews the use of confocal microscopy as it pertains to the identification of G-protein coupled receptors and the study of their dynamic properties in cell cultures and in mammalian brain following their tagging with specific fluorescent ligands. Principles that should guide the choice of suitable ligands and fluorophores are discussed. Examples are provided from the work carried out in the authors' laboratory using custom synthetized fluoresceinylated or BODIPY-tagged bioactive peptides. The results show that confocal microscopic detection of specifically bound fluorescent ligands permits high resolution appraisal of neuropeptide receptor distribution both in cell culture and in brain sections. Within the framework of time course experiments,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurotensin; Somatostatin; Opioids; Internalization; Endocytosis; Confocal microscopy.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100017

Effect of hydroxyurea on the intracellular multiplication of Toxoplasma gondii, Leishmania amazonensis and Trypanosoma cruzi
Provedor de dados: 56
Autores: Melo,E.J.T.; Beiral,H.J.
Toxoplasma gondii, Leishmania amazonensis and Trypanosoma cruzi are obligate intracellular parasites that multiply until lysis of host cells. The present study was undertaken to evaluate the effect of hydroxyurea (an inhibitor of cell division at the G1/S phase) on the multiplication of L. amazonensis, T. gondii, and T. cruzi in infected host cells. Infected cells were treated with hydroxyurea (4 mM) for 48 h. Hydroxyurea arrested intracellular multiplication of all infective forms of the parasites tested. In treated cultures, the percent of infected host cells decreased (50-97%) and most intracellular parasites were eliminated. Ultrastructural observations showed no morphologic change in host cells while intracellular parasites presented drastic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Toxoplasma gondii; Trypanosoma cruzi; Leishmania amazonensis; Hydroxyurea.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100009

Antinociceptive effects of Cremophor EL orally administered to mice
Provedor de dados: 56
Autores: Tabarelli,Z.; Berlese,D.B.; Sauzem,P.D.; Mello,C.F.; Rubin,M.A.
Surfactants are frequently used to improve solubilization of lipophilic drugs. Cremophor EL (CrEL) is a polyoxyethylated castor oil surfactant used to solubilize water-insoluble drugs such as anesthetic, antineoplastic, immunosuppressive and analgesic drugs, vitamins and new synthetic compounds, including potential analgesics. The antinociceptive effect of CrEL (3.2, 6.4 and 10.6 g/kg, in 10 ml/kg body weight, by gavage) on the abdominal writhing response induced by intraperitoneal administration of acetic acid (0.8%, 10 ml/kg body weight) and on the tail immersion test was investigated in mice. Control animals received castor oil (10 ml/kg body weight) or saline (0.9% NaCl, 10 ml/kg body weight). CrEL reduced nociception in a dose-dependent manner in both...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Antinociception; Cremophor EL; Tail immersion test; Writhing test; Dipyrone; Morphine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100016

Effects of melatonin on DNA damage induced by cyclophosphamide in rats
Provedor de dados: 56
Autores: Ferreira,S.G.; Peliciari-Garcia,R.A.; Takahashi-Hyodo,S.A.; Rodrigues,A.C.; Amaral,F.G.; Berra,C.M.; Bordin,S.; Curi,R.; Cipolla-Neto,J.
The antioxidant and free radical scavenger properties of melatonin have been well described in the literature. In this study, our objective was to determine the protective effect of the pineal gland hormone against the DNA damage induced by cyclophosphamide (CP), an anti-tumor agent that is widely applied in clinical practice. DNA damage was induced in rats by a single intraperitoneal injection of CP (20 or 50 mg/kg). Animals received melatonin during the dark period for 15 days (1 mg/kg in the drinking water). Rat bone marrow cells were used for the determination of chromosomal aberrations and of formamidopyrimidine DNA glycosylase enzyme (Fpg)-sensitive sites by the comet technique and ofXpf mRNA expression by qRT-PCR. The number (mean ± SE) of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melatonin; Cyclophosphamide; Chromosomal aberration; DNA fragmentation; Comet assay; Xpf expression.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300278

Swimming training attenuates oxidative damage and increases enzymatic but not non-enzymatic antioxidant defenses in the rat brain
Provedor de dados: 56
Autores: Nonato,L.F.; Rocha-Vieira,E.; Tossige-Gomes,R.; Soares,A.A.; Soares,B.A.; Freitas,D.A.; Oliveira,M.X.; Mendonça,V.A.; Lacerda,A.C.; Massensini,A.R.; Leite,H.R.
Although it is well known that physical training ameliorates brain oxidative function after injuries by enhancing the levels of neurotrophic factors and oxidative status, there is little evidence addressing the influence of exercise training itself on brain oxidative damage and data is conflicting. This study investigated the effect of well-established swimming training protocol on lipid peroxidation and components of antioxidant system in the rat brain. Male Wistar rats were randomized into trained (5 days/week, 8 weeks, 30 min; n=8) and non-trained (n=7) groups. Forty-eight hours after the last session of exercise, animals were euthanized and the brain was collected for oxidative stress analysis. Swimming training decreased thiobarbituric acid reactive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise therapy; Oxidative stress; Rat; Lipid peroxidation; Superoxide dismutase; Swimming training.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000605

Prostanoids modulate inflammation and alloimmune responses during graft rejection
Provedor de dados: 56
Autores: Rocha,P.N.; Carvalho,E.M.
Acute rejection of a transplanted organ is characterized by intense inflammation within the graft. Yet, for many years transplant researchers have overlooked the role of classic mediators of inflammation such as prostaglandins and thromboxane (prostanoids) in alloimmune responses. It has been demonstrated that local production of prostanoids within the allograft is increased during an episode of acute rejection and that these molecules are able to interfere with graft function by modulating vascular tone, capillary permeability, and platelet aggregation. Experimental data also suggest that prostanoids may participate in alloimmune responses by directly modulating T lymphocyte and antigen-presenting cell function. In the present paper, we provide a brief...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thromboxane; Prostaglandins; Transplantation; Inflammation; Rejection; Corticosteroids.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200004

HFE gene mutations in Brazilian thalassemic patients
Provedor de dados: 56
Autores: Oliveira,T.M.; Souza,F.P.; Jardim,A.C.G.; Cordeiro,J.A.; Pinho,J.R.R.; Sitnik,R.; Estevão,I.F.; Bonini-Domingos,C.R.; Rahal,P.
Hereditary hemochromatosis is a disorder of iron metabolism characterized by increased iron intake and progressive storage and is related to mutations in the HFE gene. Interactions between thalassemia and hemochromatosis may further increase iron overload. The ethnic background of the Brazilian population is heterogeneous and studies analyzing the simultaneous presence of HFE and thalassemia-related mutations have not been carried out. The aim of this study was to evaluate the prevalence of the H63D, S65C and C282Y mutations in the HFE gene among 102 individuals with alpha-thalassemia and 168 beta-thalassemia heterozygotes and to compare them with 173 control individuals without hemoglobinopathies. The allelic frequencies found in these three groups were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HFE; H63D; S65C; C282Y; Thalassemia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200008

Leishmania infantum heat shock protein 83 for the serodiagnosis of tegumentary leishmaniasis
Provedor de dados: 56
Autores: Celeste,B.J.; Angel,S.O.; Castro,L.G.M.; Gidlund,M.; Goto,H.
The serologic assay is an important tool in the diagnosis of leishmaniasis. One of the most commonly used tests is enzyme-linked immunosorbent assay (ELISA). Since total Leishmania promastigotes are used as antigen in the routine assay, false-positive reactions are frequent due to cross-reaction with sera from other diseases, mainly Chagas' disease. Therefore, an antigen that determines less cross-reactivity has been pursued for the serodiagnosis of leishmaniasis. In the present study we analyzed the use of recombinant Leishmania infantum heat shock protein (Hsp) 83 in ELISA for the serodiagnosis of cutaneous (N = 12) and mucocutaneous leishmaniasis (N = 14) and we observed the presence of anti-L. infantum Hsp 83 antibodies in all samples as well as...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leishmania infantum; Sp 83; ELISA; Serodiagnosis; Tegumentary leishmaniasis; Chagas' disease.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100001

Association of sulfur dioxide exposure with circulatory system deaths in a medium-sized city in Brazil
Provedor de dados: 56
Autores: Amancio,C.T.; Nascimento,L.F.C.
There is a demonstrable association between exposure to air pollutants and deaths due to cardiovascular diseases. The objective of this study was to estimate the effects of exposure to sulfur dioxide on mortality due to circulatory diseases in individuals 50 years of age or older residing in São José dos Campos, SP. This was a time-series ecological study for the years 2003 to 2007 using information on deaths due to circulatory disease obtained from Datasus reports. Data on daily levels of pollutants, particulate matter, sulfur dioxide (SO2), ozone, temperature, and humidity were obtained from the São Paulo State Environmental Agency. Moving average models for 2 to 7 days were calculated by Poisson regression using the R software. Exposure to SO2 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Air pollution; Sulfur dioxide; Cardiovascular diseases; Mortality; Air pollutants; Stroke.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100013

Effect of injection of L-NAME on drinking response
Provedor de dados: 56
Autores: Saad,W.A.; Camargo,L.A.A.; Saad,R.; Pereira,A.F.; Simões,S.
The drinking behavior responses to centrally administered NG-nitro-L-arginine methyl ester (L-NAME; 10, 20 or 40 µg/µl), an inhibitor of nitric oxide synthase, were studied in satiated rats, with cannulae stereotaxically implanted into the lateral ventricle (LV) and subfornical organ (SFO). Water intake increased in all animals after angiotensin II (ANG II) injection into the LV, with values of 14.2 ± 1.4 ml/h. After injection of L-NAME at doses of 10, 20 or 40 µg/µl into the SFO before injection of ANG II (12 ng/µl) into the LV, water intake decreased progressively and reached basal levels after treatment with 0.15 M NaCl and with the highest dose of L-NAME (i.e., 40 µg). The water intake obtained after 40 µg/µl L-NAME was 0.8 ± 0.01 ml/h. Also, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Water intake; SFO; Dehydration; CNS.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100012

Induction of chagasic-like arrhythmias in the isolated beating hearts of healthy rats perfused with Trypanosoma cruzi-conditioned medium
Provedor de dados: 56
Autores: Rodríguez-Angulo,H.; Toro-Mendoza,J.; Marques,J.; Bonfante-Cabarcas,R.; Mijares,A.
Chagas' myocardiopathy, caused by the intracellular protozoan Trypanosoma cruzi, is characterized by microvascular alterations, heart failure and arrhythmias. Ischemia and arrythmogenesis have been attributed to proteins shed by the parasite, although this has not been fully demonstrated. The aim of the present investigation was to study the effect of substances shed by T. cruzi on ischemia/reperfusion-induced arrhythmias. We performed a triple ischemia-reperfusion (I/R) protocol whereby the isolated beating rat hearts were perfused with either Vero-control or Vero T. cruzi-infected conditioned medium during the different stages of ischemia and subsequently reperfused with Tyrode's solution. ECG and heart rate were recorded during the entire experiment. We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chagas' disease; Arrhythmias; Ischemia.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100058

Spirometry reference values in the Brazilian population
Provedor de dados: 56
Autores: Rufino,R.; Costa,C.H.; Lopes,A.J.; Maiworm,A.I.; Maynard,K.; Silva,L.M.R.A.; Dias,R.M.
The aim of the present study was to provide new spirometry reference equations in a sample of the Brazilian population for the following parameters: forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), FEV1/FVC ratio, peak of expiratory flow (PEF), forced expiratory flow at 50% (FEF50%), 75% average vital capacity (FEF25-75%), and average forced expiratory flow time (FEFT). This was a prospective study using results from chest radiographs, electrocardiograms, and questionnaires to investigate the participants' respiratory symptoms, sedentarism, and comorbidities (Charlson comorbidity index). From December 2010 to July 2014, individuals were randomly selected from various locations in the state of Rio de Janeiro. All individuals were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spirometry; Lung function test; Reference values; FVC; FEV1.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300703

Evidence of hemispheric specialization in marmosets (Callithrix penicillata) using tympanic membrane thermometry
Provedor de dados: 56
Autores: Tomaz,C.; Verburg,M.S.; Boere,V.; Pianta,T.F.; Belo,M.
Recent studies have employed tympanic thermometry to assess lateralization of cognitive and emotional functions in primates. However, no studies using this technique have investigated the possibility of hemispheric specialization in New World monkeys. Therefore, the aim of the present study was to investigate tympanic membrane (TM) temperature asymmetries and their possible correlation with stress responses in marmosets (Callithrix penicillata). Infrared TM thermometry was completed bilaterally in 24 animals (14 males and 10 females) during a stressful situation of capture and restraint. There were no significant differences between gender. A significant negative correlation was observed between TM temperature of the right ear and the number of captures (r...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laterality; Tympanic temperature; Stress; New World monkey.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700012

Application of continuous positive airway pressure in the delivery room: a multicenter randomized clinical trial
Provedor de dados: 56
Autores: Gonçalves-Ferri,W.A.; Martinez,F.E.; Caldas,J.P.S.; Marba,S.T.M.; Fekete,S.; Rugolo,L.; Tanuri,C.; Leone,C.; Sancho,G.A.; Almeida,M.F.B.; Guinsburg,R.
This study evaluated whether the use of continuous positive airway pressure (CPAP) in the delivery room alters the need for mechanical ventilation and surfactant during the first 5 days of life and modifies the incidence of respiratory morbidity and mortality during the hospital stay. The study was a multicenter randomized clinical trial conducted in five public university hospitals in Brazil, from June 2008 to December 2009. Participants were 197 infants with birth weight of 1000-1500 g and without major birth defects. They were treated according to the guidelines of the American Academy of Pediatrics (APP). Infants not intubated or extubated less than 15 min after birth were randomized for two treatments, routine or CPAP, and were followed until hospital...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Continuous positive airway pressure (CPAP) ventilation; Preterm infants; Delivery room; Nasal CPAP; Prematurity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300259

The role of osteoblasts in regulating hematopoietic stem cell activity and tumor metastasis
Provedor de dados: 56
Autores: Neiva,K.; Sun,Y.-X.; Taichman,R.S.
Bone marrow stromal cells are critical regulators of hematopoiesis. Osteoblasts are part of the stromal cell support system in bone marrow and may be derived from a common precursor. Several studies suggested that osteoblasts regulate hematopoiesis, yet the entire mechanism is not understood. It is clear, however, that both hematopoietic precursors and osteoblasts interact for the production of osteoclasts and the activation of resorption. We observed that hematopoietic stem cells (HSCs) regulate osteoblastic secretion of various growth factors, and that osteoblasts express some soluble factors exclusively in the presence of HSCs. Osteoblasts and hematopoietic cells are closely associated with each other in the bone marrow, suggesting a reciprocal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoblasts; Hematopoietic stem cells; Bone marrow; Stromal-derived factor-1; CXCR4; Prostate cancer.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000001

Role of angiotensin II and vasopressin receptors within the supraoptic nucleus in water and sodium intake induced by the injection of angiotensin II into the medial septal area
Provedor de dados: 56
Autores: Antunes,V.R.; Camargo,G.M.P.A.; Saad,R.; Saad,W.A.; Luiz,A.C.; Camargo,L.A.A.
In this study we investigated the effects of the injection into the supraoptic nucleus (SON) of non-peptide AT1- and AT2-angiotensin II (ANG II) receptor antagonists, DuP753 and PD123319, as well as of the arginine-vasopressin (AVP) receptor antagonist d(CH2)5-Tyr(Me)-AVP, on water and 3% NaCl intake induced by the injection of ANG II into the medial septal area (MSA). The effects on water or 3% NaCl intake were assessed in 30-h water-deprived or in 20-h water-deprived furosemide-treated adult male rats, respectively. The drugs were injected in 0.5 µl over 30-60 s. Controls were injected with a similar volume of 0.15 M NaCl. Antagonists were injected at doses of 20, 80 and 180 nmol. Water and sodium intake was measured over a 2-h period. Previous...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Angiotensin II; AT1 receptors; AT2 receptors; V1 receptors; Water intake; Sodium intake; Medial septal area; Supraoptic nucleus; Rats.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200013

Effect of image resolution manipulation in rearfoot angle measurements obtained with photogrammetry
Provedor de dados: 56
Autores: Sacco,I.C.N.; Picon,A.P.; Ribeiro,A.P.; Sartor,C.D.; Camargo-Junior,F.; Macedo,D.O.; Mori,E.T.T.; Monte,F.; Yamate,G.Y.; Neves,J.G.; Kondo,V.E.; Aliberti,S.
The aim of this study was to investigate the influence of image resolution manipulation on the photogrammetric measurement of the rearfoot static angle. The study design was that of a reliability study. We evaluated 19 healthy young adults (11 females and 8 males). The photographs were taken at 1536 pixels in the greatest dimension, resized into four different resolutions (1200, 768, 600, 384 pixels) and analyzed by three equally trained examiners on a 96-pixels per inch (ppi) screen. An experienced physiotherapist marked the anatomic landmarks of rearfoot static angles on two occasions within a 1-week interval. Three different examiners had marked angles on digital pictures. The systematic error and the smallest detectable difference were calculated from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Physical therapy modalities; Photogrammetry; Reliability; Rearfoot angle.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900003

Introduction to III Forum on the Neurobiology of Stress, Araraquara, SP, Brazil, September 8-10, 2011
Provedor de dados: 56
Autores: Brandão,Marcus Lira.
Tipo: Info:eu-repo/semantics/other
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400001

Contrast sensitivity threshold measured by sweep-visual evoked potential in term and preterm infants at 3 and 10 months of age
Provedor de dados: 56
Autores: Oliveira,A.G.F.; Costa,M.F.; Souza,J.M. de; Ventura,D.F.
Although healthy preterm infants frequently seem to be more attentive to visual stimuli and to fix on them longer than full-term infants, no difference in visual acuity has been reported compared to term infants. We evaluated the contrast sensitivity (CS) function of term (N = 5) and healthy preterm (N = 11) infants at 3 and 10 months of life using sweep-visual evoked potentials. Two spatial frequencies were studied: low (0.2 cycles per degrees, cpd) and medium (4.0 cpd). The mean contrast sensitivity (expressed in percentage of contrast) of the preterm infants at 3 months was 55.4 for the low spatial frequency (0.2 cpd) and 43.4 for the medium spatial frequency (4.0 cpd). At 10 months the low spatial CS was 52.7 and the medium spatial CS was 9.9. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Contrast sensitivity; Preterm infants; Sweep-visual evoked potential; Visual development; Visual acuity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900014

The effect of bacterial lipopolysaccharide on gastric emptying in rats suffering from moderate renal insufficiency
Provedor de dados: 56
Autores: Rigatto,S.Z.P.; Collares,E.F.
The objective of the present study was to evaluate the response of rats suffering from moderate renal insufficiency to bacterial lipopolysaccharide (LPS, or endotoxin). The study involved 48 eight-week-old male SPF Wistar rats (175-220 g) divided into two groups of 24 animals each. One group underwent 5/6 nephrectomy while the other was sham-operated. Two weeks after surgery, the animals were further divided into two subgroups of 12 animals each and were fasted for 20 h but with access to water ad libitum. One nephrectomized and one sham-treated subgroup received E. coli LPS (25 µg/kg, iv) while the other received a sterile, pyrogen-free saline solution. Gastric retention (GR) was determined 10 min after the orogastric infusion of a standard saline test...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric emptying; Lipopolysaccharide; Endotoxin; Renal insufficiency; Rats.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400006

In vitro modulation of alkaline phosphatase activity of Saccharomyces cerevisiae grown in low or high phosphate medium
Provedor de dados: 56
Autores: Fernandes,J; Amorim,R; Azevedo,I; Martins,M.J.
Our objective was to characterize the modulation of the activity of Saccharomyces cerevisiae alkaline phosphatases (ALPs) by classic inhibitors of ALP activity, cholesterol and steroid hormones, in order to identify catalytic similarities between yeast and mammalian ALPs. S. cerevisiae expresses two ALPs, coded for by the PHO8 and PHO13 genes. The product of the PHO8 gene is repressible by Pi in the medium. ALP activity from yeast (grown in low or high phosphate medium) homogenates was determined with p-nitrophenylphosphate as substrate, pH 10.4 (lPiALP or hPiALP, respectively). Activation of hPiALP was observed with 5 mM L-amino acids (L-homoarginine _ 186%, L-leucine _ 155% and L-phenylalanine - 168%) and with 1 mM levamisole (122%; percentage values, in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharomyces cerevisiae; Alkaline phosphatase inhibitors; PHO8 and PHO13; Cholesterol; Steroid hormones.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100007

Paradoxical effect of a pequi oil-rich diet on the development of atherosclerosis: balance between antioxidant and hyperlipidemic properties
Provedor de dados: 56
Autores: Aguilar,E.C.; Jascolka,T.L.; Teixeira,L.G.; Lages,P.C.; Ribeiro,A.C.C.; Vieira,E.L.M.; Peluzio,M.C.G.; Alvarez-Leite,J.I.
Pequi is the fruit of Caryocar brasiliense and its oil has a high concentration of monounsaturated and saturated fatty acids, which are anti- and pro-atherogenic agents, respectively, and of carotenoids, which give it antioxidant properties. Our objective was to study the effect of the intake of a cholesterol-rich diet supplemented with pequi oil, compared to the same diet containing soybean oil, on atherosclerosis development, and oxidative stress in atherosclerosis-susceptible LDL receptor-deficient mice (LDLr-/-, C57BL/6-background). Female mice were fed a cholesterol-rich diet containing 7% soybean oil (Soybean group, N = 12) or 7% pequi oil (Pequi group, N = 12) for 6 weeks. The Pequi group presented a more atherogenic lipid profile and more advanced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pequi oil; Fatty acid; Lipids; Atherosclerosis; Oxidative stress; Cholesterol.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700006

Antisense mRNA for NPY-Y1 receptor in the medial preoptic area increases prolactin secretion
Provedor de dados: 56
Autores: Silveira,N.A.; Franci,C.R.
We investigated the participation of neuropeptide Y-Y1 receptors within the medial preoptic area in luteinizing hormone, follicle-stimulating hormone and prolactin release. Four bilateral microinjections of sense (control) or antisense 18-base oligonucleotides of messenger ribonucleic acid (mRNA) (250 ng) corresponding to the NH2-terminus of the neuropeptide Y1 receptor were performed at 12-h intervals for two days into the medial preoptic area of ovariectomized Wistar rats (N = 16), weighing 180 to 200 g, treated with estrogen (50 µg) and progesterone (25 mg) two days before the experiments between 8.00 and 10:00 a.m. Blockade of Y1 receptor synthesis in the medial preoptic area by the antisense mRNA did not change plasma luteinizing hormone or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuropeptide Y; Medial preoptic area; Neuropeptide Y-Y1 receptor; Prolactin; MRNA for neuropeptide; Y-Y1 receptor; Luteinizing hormone; Follicle-stimulating hormone.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900016

Eosinophil-active chemokines: assessment of in vivo activity
Provedor de dados: 56
Autores: Teixeira,M.M.
The selective recruitment of eosinophils in tissue is a striking feature of allergic diseases. Recently, a family of chemoattractant molecules, namely chemokines, has been described which potently activates eosinophil function in vitro. We have developed a murine model of eosinophil recruitment to compare the relative potency and efficacy of chemokines in vivo. Of the chemokines tested, only eotaxin and MIP-1&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; induced significant accumulation of eosinophils in vivo, but eotaxin was more effective than MIP-1&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;. Chemokines, especially eotaxin acting via the CCR-3 receptor, may have a fundamental role in determining selective eosinophil recruitment in vivo
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eosinophils chemokines eotaxin MIP-1&lt; FONT FACE=Symbol&gt; A&lt; /font&gt; Chemokine receptors.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100003