Consulta: subjectFacets:"ELISA"
Registros recuperados: 174
Data/hora: 03/06/2024 01:19:46

Bt protein rhizosecreted from transgenic maize does not accumulate in soil
Provedor de dados: 69
Autores: Margarit,Ezequiel; Reggiardo,Martín I; Permingeat,Hugo R.
The persistence of CryIAb protein rhizosecreted in soil is important in the assessment of its environmental risk. Here we report that CryIAb protein from transgenic maize does not accumulate at high levels in soils. Levels of CryIAb protein rhizosecreted by three maize transgenic events (BT11, MON810 and 176) were studied in hydroponic cultures and found only in the MON810 and BT11 events but not in event 176 or control plants. Under field conditions, the cryIAb gene and a basal level of CryIAb protein was detected in soils from plots cultivated with transgenic and non-transgenic maize, possibly from Bacillus thuringiensis present in the soils.
Tipo: Journal article
Palavras-chave: CryIAb gene; CryIAb protein; ELISA; Genetically modified organisms; PCR; Zea mays (L.).
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200004

Expression of Hemagglutinin-Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco
Provedor de dados: 69
Autores: Ghaffar Shahriari,Amir; Bagheri,Abdolreza; Bassami,Mohammad Reza; Malekzadeh-Shafaroudi,Saeid; Afsharifar,Alireza; Niazi,Ali.
Background: Newcastle disease is an important avian infectious disease that brings about vast economic damage for poultry industry. Transgenic plants represent a cost-effective system for the production of therapeutic proteins and are widely used for the production of poultry vaccines. In an attempt to develop a recombinant vaccine, a plant expression binary vector pBI121, containing the genes encoding Hemagglutinin-Neuraminidase (HN) and Fusion (F) epitopes of Newcastle Disease Virus (NDV) under the control of CaMV35S promoter and NOS terminator was constructed and introduced into the tobacco ( Nicotiana tabacum) plant by Agrobacterium-mediated transformation. Results: Putative transgenic plants were screened in a selection medium containing 50 mg/L...
Tipo: Journal article
Palavras-chave: Agrobacterium tumefaciens; ELISA; Recombinant vaccine; Transgenic plants.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400006

Heterologous expression of staphylococcal enterotoxin B (seb) gene for antibody production
Provedor de dados: 69
Autores: Vrat Kamboj,Dev; Nema,Vijay; Kumar Pandey,Arun; Kumar Goel,Ajay; Singh,Lokendra.
Staphylococcal food poisoning (SFP) is caused by the members of superantigen family called staphylococcal enterotoxins (SEs). About 20 different types of SEs are produced by Staphylococcus aureus out of which type A (SEA), B (SEB), C (SEC) and D (SED) are commonly implicated in SFP. Among these, SEB is the most potent toxin and has also gained the status of biological warfare (BW) agent. Therefore, detection of SEB is of utmost importance. Any immunological detection system for SEB requires specific and sensitive antibodies which inturn depends on the purity of the SEB. In the present investigation, seb gene of S. aureus was cloned and expressed in E. coli along with biotin as fusion partner to facilitate the purification process. The yield of purified...
Tipo: Journal article
Palavras-chave: Affinity purification; Biotin fusion; ELISA; Gene cloning; SEB; Western blot.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500010

Production of polyclonal antisera to manganese superoxide dismutase expressed in downy mildew resistant pearl millet and its application for immunodiagnosis
Provedor de dados: 69
Autores: Urs Babitha,Mysore Pandurangaraj; Madhu,Devaiah; Sripathi P,Harishchandra; Shekar Shetty,Hunthrike.
Level of superoxide dismutase (SOD) in the seedlings of downy mildew resistant pearl millet genotype (IP 18293) increased 3-fold upon inoculation with Sclerospora graminicola. Mn-SOD was purified from pearl millet by ion-exchange and gel filtration chromatography. Polyclonal antibody was raised in rabbit against Mn-SOD of pearl millet. Anti-Mn SOD had an antibody titer of 1:20,000. ELISA results revealed a 1.5-fold increase in pure Mn-SOD preparation compared to the crude preparation. The antibody reactivity as detected by ELISA revealed that Mn-SOD is higher at 4 hrs post-inoculation in resistant pearl millet seedlings. The reactivity also showed the Mn-SOD is more in roots of inoculated resistant seedlings. SOD reactivity was also determined by DIBA...
Tipo: Journal article
Palavras-chave: DIBA; Downy mildew; ELISA; Immunofluorescence; Manganese superoxide dismutase; Pearl millet; Sclerospora graminicola; Western blot.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200007

Dot-Blot: alternativa para o diagnóstico da artrite encefalite caprina (AEC).
Provedor de dados: 14
Autores: PINHEIRO, R. R.; GOUVEIA, A. M. G.; OLORTEGUI, C. C.; ANDRIOLI, A.; ARAÚJO, S. C.
bitstream/item/26723/1/COT-57.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: CAE; Artrite encefalite caprina; Affinity choromatography; Dot-Blot; Immunoenzyme techniques.; Caprine arthritis encephalit virus; Caprino; Doença animal; Epidemiologia; Técnica imunoenzimática; ELISA; Goats; Lentivirus; Epidemiology; Disease control.
Ano: 2001
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/528419

Recherche et caractérisation d'immunoglobulines dans les oeufs du loup (Dicentrarchus labrax)
Provedor de dados: 5
Autores: Pepin, Jean-francois; Breuil, Gilles; Rigobert, P; Romestand, Bernard.
The occurrence of maternal immunity transfer in Sea bass (Dicentrarchus labrax) was investigated using ELISA and Western blot technics. In order to determine the possible presence of immunoglobulin (Ig), egg proteins obtained from fertilized eggs were analyzed. After several extraction protocol assays (precipitation, chromatography), monoclonal and polyclonal antibody probes specific to Sea bass Ig allowed to confirm the presence of an immunoglobulin-like protein in eggs with very low level. Biochemical characterization was carried out using SDS-PAGE. Results indicated that egg Ig had a molecular weight of 206 kDa. They were smaller than seric IgM (800 kDa). The heavy (H) and light (L) chains of the egg Ig and of the seric IgM had similar molecular weight,...
Tipo: Text
Palavras-chave: Immunoglobuline; Transfert immunité maternelle; ELISA; Œuf; Poisson; Immunoglobulin; Maternal immunity transfer; ELISA; Eggs; Fish.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00272/38275/36458.pdf

Gametogenetic cycle and reproductive effort assessed by two methods in 3 age classes of Pacific oysters, Crassostrea gigas, reared in Normandy
Provedor de dados: 5
Autores: Royer, J; Seguineau, Catherine; Park, K; Pouvreau, Stephane; Choi, K; Costil, K.
Two methods were used to estimate the reproductive output of female Pacific oysters reared in Normandy: histology with image analysis and ELISA (Enzyme-Linked lmmunoSorbent Assay) which allowed the quantification of egg protein. Condition indices, gonad area and gametogenetic stages of the oysters were determined in the entire population (males and females) between May and October 2005. All investigations were performed in 3 age classes: oysters in their first, second or third years (corresponding to spat, half-grown and market-sized oysters, respectively). Both quantitative histology and ELISA provided similar results in terms of reproductive effort (illustrated by the gonado-somatic index, GSI) except during the GSI drop, corresponding to spawning, which...
Tipo: Text
Palavras-chave: Reproductive effort; Image analysis; Gametogenetic cycle; ELISA; Crassostrea gigas; Condition index.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4305.pdf

Contribution à l'étude du système neuroendocrine de la moule Mytilus edulis (Mollusca: Bivalvia)
Provedor de dados: 5
Autores: Kellner-cousin, Kristell.
Dans le cadre de ce travail, l'étude du système neuroendocrine de la moule Mytilus edulis a été envisagée sous plusieurs aspects qui définissent les quatre grandes parties de la thèse.  Dans une première partie est décrit le travail de préparation et de caractérisation d'anticorps monoclonaux (AcMc) spécifiques de ganglions cérébroïdes (GC) de Mytilus edulis. Le développement de techniques de préparation d'AcMc dirigés contre des antigènes non purifiés a en effet permis de produire un ensemble de réactifs de type AcMc dirigés contre différents antigènes issus d'un broyat de GC de moule. La spécificité de ces AcMc a été étudiée en microscopie optique puis électronique selon des techniques immunocytochimiques (immunopéroxydase indirecte et immunogold). La...
Tipo: Text
Palavras-chave: Mollusque; Bivalve; Mytilus edulis; Cellules neurosécrétrices; Système nerveux; Système immunitaire; Controle de la croissance; Insuline; Anticorps monoclonaux; Immunocytocbimie; ELISA; PCR; Banque d'ADNc.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00109/22019/19642.pdf

Rapid enzyme-linked immunosorbent assay for detection of the algal toxin domoic acid
Provedor de dados: 5
Autores: Litaker, R. Wayne; Stewart, Thomas N.; Eberhart, Bich-thuy L.; Wekell, John C.; Trainer, Vera L.; Kudela, Raphael M.; Miller, Peter E.; Roberts, Alice; Hertz, Cassandra; Johnson, Tyler A.; Frankfurter, Greg; Smith, G. Jason; Schnetzer, Astrid; Schumacker, Joe; Bastian, Jonnette L.; Odell, Anthony; Gentien, Patrick; Le Gal, Dominique; Hardison, I. Ransom; Tester, Patricia A.
Domoic acid (DA) is a potent toxin produced by bloom-forming phytoplankton in the genus which is responsible for causing amnesic shellfish poisoning (ASP) in humans. ASP symptoms include vomiting. diarrhea, and in more severe cases confusion. loss of memory. disorientation. and even coma or death. This paper describes the development and validation of a rapid. sensitive. enzyme linked immunosorbent assay test kit for detecting DA using a monoclonal antibody. The assay gives equivalent results to those obtained using standard high performance liquid chromatography. fluorenylmetbox-yearbonyl high performance liquid chromatography, or liquid chromatography-mass spectrometry methods. It has a linear range from 1.1-3 ppb and was used successfully to measure DA...
Tipo: Text
Palavras-chave: Test kit; Razor clams; Scallops; Mussels; ELISA; Domoic acid poisoning; ASP.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-6253.pdf

Temporal study of estrogenic responses of mussel (Mytilus galloprovinciallis) extracts applied to reporter cell lines
Provedor de dados: 5
Autores: David, A; Dagnino, S; Pichot, Yves; Munaron, Dominique; Escande, A; Casellas, C; Fenet, H; Gomez, E.
In vitro bioassays would facilitate monitoring of estrogen-like compounds in mussels (Mytilus galloprovincialis) since they tend to accumulate lipophilic compounds in their fat and muscle tissue. However, estradiol (E2) steroid (already identified in mussels) could induce false positive responses. This study focuses on temporal variability in estrogenic responses of mussel extracts and the possible relation of this response with E2 levels. The reproductive cycle and growth were thus monitored for six months in sexed mussels. E2 levels were similar between genders and these levels varied with reproductive stages. human estrogen receptor (hER) activities were similar at all stages except in February. E2 present in male and female extracts accounted for part...
Tipo: Text
Palavras-chave: ELISA; Reporter cell lines; Mytilus galloprovinciallis; Estradiol; Biomonitoring.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4578.pdf

Stabilising selection on immune response in male black grouse Lyrurus tetrix
Provedor de dados: 5
Autores: Soulsbury, Carl D.; Siitari, Heli; Lebigre, Christophe.
Illnesses caused by a variety of micro- and macro- organisms can negatively affect individuals' fitness, leading to the expectation that immunity is under positive selection. However, immune responses are costly and individuals must trade-off their immune response with other fitness components (e.g. survival or reproductive success) meaning that individuals with intermediate response may have the greatest overall fitness. Such a process might be particularly acute in species with strong sexual selection because the condition-dependence of male secondary sexual-traits might lead to striking phenotypic differences amongst males of different immune response levels. We tested whether there is selection on immune response by survival and reproduction in...
Tipo: Text
Palavras-chave: Ecological immunology; ELISA; Immunocompetance; Life history theory; Stabilising selection.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00426/53761/54653.pdf

Sea bass Dicentrarchus labrax nervous necrosis virus isolates with distinct pathogenicity to sea bass larvae
Provedor de dados: 5
Autores: Breuil, Gilles; Mouchel, Olivier; Fauvel, Christian; Pepin, Jean-francois.
Reproduction of nodavirus disease was performed by experimental infection of sea bass eggs during fertilization or at larval stage 4 with 2 genetically distinguishable nodavirus strains (Sb1 and Sb2) isolated from sea bass collected along the Atlantic and Mediterranean French coast. The pathogenicity of the virus strains was assigned after detection of the virus by ELISA and immunohistochemistry (IHC). The Atlantic (Sb1) strain was more pathogenic than the Mediterranean (Sb2) strain during the fertilization step whilst both strains were pathogenic following experimental exposure of 4 d old larvae. Virus lesions developed in the brain 4 to 6 d following experimental exposure. Experimental ELISA proved very sensitive for detecting the nodavirus in Sb1 or Sb2...
Tipo: Text
Palavras-chave: ELISA; Nodavirus; Fish encephalopathy.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-814.pdf

Quantification of eggs and sperm in the Black-lip pearl oyster Pinctada margaritifera using an enzyme-linked immunosorbent assay (ELISA)
Provedor de dados: 5
Autores: Jeung, Hee-do; Kang, Do-hyung; Park, Heung-sik; Le Moullac, Gilles; Choi, Kwang-sik.
We have developed immunological probes to quantify eggs and sperm of the Black-lip pearl (BLP) oyster Pinctada margaritifera. The western blot assay revealed that the polyclonal antibodies developed in this study specifically recognized only egg and sperm proteins. These polyclonal antibodies also showed high sensitivities to the antigens, detecting 0.31–10 μg/ml of the egg or sperm proteins in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, we used an indirect ELISA to quantify the eggs and sperm in BLP oysters collected in December 2009 from Weno Island in Micronesia and in May 2010 from Tahiti. The gonad somatic index (GSI), a ratio of gonad weight to somatic tissue weight, of the females collected from Weno Island ranged from 3.6 to...
Tipo: Text
Palavras-chave: Black-lip pearl oyster; ELISA; Pinctada margaritifera; Polyclonal antibody; Quantification of eggs and sperm.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00175/28659/32145.pdf

Humoral response and neutralization capacity of sheep serum inoculated with natural and Cobalt 60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)
Provedor de dados: 79
Autores: Netto,D. P.; Chiacchio,S. B.; Bicudo,P. L.; Alfieri,A. A.; Nascimento,N.
The aim of this work was to investigate antigen irradiation on crotalic antivenom and the capacity of sheep as serum producers. Twelve sheep in two groups of six were inoculated with Crotalus durissus terrificus venom. One group was inoculated with natural venom (NV) and the other with Cobalt 60 gamma-irradiated venom (IrV). Three antigen doses were given to the animals at monthly intervals for immunization. The toxic activity of the venom was assessed by LD50 determination in mice. Blood samples were collected weekly analyses of serum neutralization capacity and potency. At the end of the experiment, the animals were challenged with a LD50 for sheep showed no signs of envenoming. These results showed that toxicity of the irradiated venom was 4.4 times...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma irradiated crotalic antisera; Snake venom; ELISA; Sheep; Crotalus durissus terrificus; Cobalt60.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200009

TOXIGENICITY CHARACTERIZATION OF Clostridium perfringens FROM BOVINE ISOLATES
Provedor de dados: 79
Autores: BALDASSI,L.; BARBOSA,M. L.; BACH,E. E.; IARIA,S. T.
Clinical samples from 71 bovine from different Brazilian states were processed for the analysis of anaerobe organisms with emphasis on the isolation and characterization of Clostridium spp. From these, eighty-nine Clostridium perfringens strains were recovered: 32 from liver, 19 from intestinal contents, 14 from kidney, 6 from rumen, 5 from nervous system, 4 from bone marrow, 2 from udder tract, blood, spleen and lung, and one from muscle. Four reference Clostridium perfringens types A, B, C, and D were used as controls in this study. All isolates were cultivated in appropriate media, and after centrifugation the supernatant and sediment were separated. From pure supernatant post exopolysaccharide (EPS) extraction, mouse toxigenicity tests were performed,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Toxigenicity; Bovine isolates; ELISA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100010

Biotin-avidin sandwich elisa with specific human isotypes IgG1 and IgG4 for Culicidae mosquito blood meal identification from an epizootic yellow fever area in Brazil
Provedor de dados: 80
Autores: Marassá,AM; Paula,MB; Gomes,AC; Consales,CA.
With a view toward investigating the feeding behavior of Culicidae mosquitoes from an area of epizootic yellow fever transmission in the municipalities of Garruchos and Santo Antônio das Missões, Rio Grande do Sul State, Brazil, specimens were collected by aspiration from September 2005 to April 2007. The engorged females were submitted to blood meal identification by enzyme-linked immunosorbent assay (ELISA). A total of 142 blood-engorged samples were examined for human or monkey blood through species-specific IgG. Additional tests for specificity utilizing isotypes IgG1 and IgG4 of human monoclonal antibodies showed that only anti-human IgG1 was effective in recognizing blood meals of human origin. The results indicated a significant difference (p =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culicidae; ELISA; Feeding habits; Isotypes; Yellow fever.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400008

Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera
Provedor de dados: 80
Autores: Ferreira Junior,R. S.; Nascimento,N.; Martinez,J. C.; Alves,J. B.; Meira,D. A.; Barraviera,B.
ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by "in vivo" challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperimmunization; Irradiation; Crotalus durissus terrificus; ELISA; Serum neutralization; Detoxification.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300008

Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming
Provedor de dados: 80
Autores: Ferreira,J.; Bastos,M. F.; Pelizon,A. C.; Peres,C. M.; Cavalcante,D. P.; Sartori,A.
Alternative sources of anti-ophidic serum are being investigated due to the secondary effects associated with types I and II hypersensitivity reactions. In the present study we raised and evaluated the protective effect of an ovine antibothropic serum in a Swiss mice envenoming model. Ovine antiserum was obtained by immunization with seven increasing doses of bothropic venom associated with adjuvants. The neutralizing ability was tested by the lethal activity (2 LD50) neutralization and serum and splenic venom levels after antivenom administration to experimentally envenomed mice. The antiserum effect on local edema was also tested by injection of venom/antivenom mixtures into the mice footpads. Ovine antiserum neutralized lethal activity and also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararaca venom; Ovine antiserum; Venom levels; Edema; ELISA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300010

Levels of interleukins 12 (IL-12) and 13 (IL-13), hepatitis B and C serology, and blood cultures among acute myeloid leukemia (AML) patients in Egypt
Provedor de dados: 80
Autores: RAA,Nour el deen; RA,Harfoush; MM,Elgharabawy; NA,Hamed; MG,Morsi.
There is an interest in the use of IL-12 as a possible anti-cancer drug to induce immune responses and anti-IL-13 formulations to treat the undesirable effects of IL-13. Thus, the present study aimed at analyzing IL-12 and IL-13 profiles, viral hepatitis serology and blood cultures in acute myeloid leukemia (AML) patients. Forty individuals (20 without septicemia - Group A, and 20 with septicemia - Group B) and 20 healthy controls were evaluated. Hepatitis B virus antigens (HBsAg) and hepatitis C virus antibodies (HCV Ab) were quantified using commercial ELISA kits. IL-12 and IL-13 levels were estimated in culture supernatant of mitogen-stimulated peripheral blood mononuclear cells by ELISA. Significantly low IL-12 values were observed among AML patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myeloid leukemia; IL-12; IL-13; HBsAg; HCV; ELISA.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300009

Diagnosis of arboviruses using indirect sandwich IgG ELISA in horses from the Brazilian Amazon
Provedor de dados: 80
Autores: Casseb,Alexandre do Rosário; Nunes,Márcio Roberto Teixeira; Rodrigues,Sueli Guerreiro; Rosa,Elizabeth Salbé Travassos da; Casseb,Livia Medeiros Neves; Casseb,Samir Manssor Moraes; Silva,Sandro Patroca da; Rodrigues,Érika Dayane Leal; Vasconcelos,Pedro Fernando da Costa.
Background The Amazon as a whole is the largest reservoir of arboviruses worldwide, while the Brazilian Amazon hosts the largest variety of arboviruses isolated to date. In this study, the results of an indirect sandwich IgG ELISA, standardized for 19 arbovirustypes circulating among horses in Brazilian Amazon, were compared to results of the hemagglutination inhibition test. A screening test assessed the conditional probability distribution and a Pearson linear correlation test determined the correlation strength among the absorbance values recorded for viruses from the same family.Findings Sensitivity varied between 40.85 and 100%; the specificity was low and ranged from 39.71 to 67.0%; and the accuracy varied between 41 and 65.2%. The test developed in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Horses; Arboviruses; ELISA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200601

Enzymatic and immunological properties of Bungarus flaviceps (red-headed krait) venom
Provedor de dados: 80
Autores: Tan,NH; Fung,SY; Ponnudurai,G.
Bungarus flaviceps (red-headed krait) venom presents an intravenous LD50 of 0.32 μg/g and exhibits enzymatic activities similar to other Bungarus toxins. ELISA cross-reactions between anti-Bungarus flaviceps and a variety of elapid and viperid venoms were observed in the current study. Double-sandwich ELISA was highly specific, since anti-B. flaviceps serum did not cross-react with any tested venom, indicating that this assay can be used for species diagnosis in B. flaviceps bites. In the indirect ELISA, anti-B. flaviceps serum cross-reacted moderately with three different Bungarus venoms (9-18%) and Notechis scutatus venom, but minimally with other elapid and viperid toxins. The results indicated that B. flaviceps venom shares common epitopes with other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bungarus flaviceps venom; Enzymes; ELISA; Neutralization.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100014

Kinetics of venom and antivenom serum levels, clinical evaluation and therapeutic effectiveness in dogs inoculated with Crotalus durissus terrificus venom
Provedor de dados: 80
Autores: Sangiorgio,F.; Sakate,M.; Nogueira,R. M. B.; Araújo JR.,J. P.; Chavez-Olortegui,C.
This work evaluated the clinical and therapeutic aspects as well as serum levels of venom and antivenom IgG by enzyme-linked immunosorbent assay (ELISA) in experimental envenomation of dogs with Crotalus durissus terrificus venom. Twenty-eight mixed breed adult dogs were divided into four groups of seven animals each, Group I: only venom; Group II, venom + 50 ml of anti-bothropic-crotalic serum (50mg) + fluid therapy; Group III, venom + 50 ml of anti-bothropic-crotalic serum + fluid therapy + urine alkalination; Group IV, 50 ml of anti-bothropic-crotalic serum. The lyophilized venom of Crotalus durissus terrificus was reconstituted in saline solution and subcutaneously inoculated at the dose of 1mg/kg body weight. The dogs presented clinical signs of local...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus; Dogs; Clinical signs; ELISA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100008

Serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom components in a rabbit
Provedor de dados: 80
Autores: Tan,NH; Ponnudurai,G; Fung,SY.
The serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom - specifically two of its components, the major hemorrhagin (rhodostoxin) and a thrombin-like enzyme - was examined in a rabbit by double-sandwich enzyme-linked immunosorbent assay (ELISA). The animal received intramuscularly a 1.0-mg/kg dose of C. rhodostoma venom. The venom level in serum peaked 12 hours after the injection, followed by a gradual decline and finally reached low rates 72 hours after administration. The serum kinetic profile of venom components, however, did not correspond to the profile of the whole C. rhodostoma venom. The serum levels of the C. rhodostoma thrombin-like enzyme increased slowly and peaked only 48 hours post-injection. Then both thrombin-like enzyme...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calloselasma rhodostoma venom; Thrombin-like enzyme; Hemorrhagin; Serum kinetics; Rabbit; ELISA.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200014

Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms
Provedor de dados: 80
Autores: Ferreira Junior,R. S.; Nascimento,N.; Martinez,J. C.; Alves,J. B.; Meira,D. A.; Barraviera,B.
ELISA was used to evaluate, accompany, and compare the humoral immune response of Swiss mice during hyperimmunization with native and Cobalt-60-irradiated (60Co) venoms of Bothrops jararaca, Bothrops jararacussu and Bothrops moojeni. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge, and the side effects were assessed. The animals immunization with one LD50 of each venom occurred on days 1, 15, 21, 30, and 45, when blood samples were collected; challenges happened on the 60th day. Results showed that ELISA was efficient in evaluating, accompanying and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venom; Irradiation; Bothrops; ELISA; Serum production; Detoxification.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400006

Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)
Provedor de dados: 80
Autores: Meira,DA; Almeida,RAMB; Barbosa,AN; de Souza,LR; Olivo,TET; Henriques,RMS; Golim,MA; Araújo Jr,JP; Nagoshi,LR; Orikaza,CM; Calvi,SA.
A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G1 = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G2 = 27); and patients on HAART with undetectable VL for at least the past six months (G3 = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G4 = 20), which was the control group. Serum cytokine levels (values in pg/mL) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RT-PCR; ELISA; Cytokines; HIV; AIDS; HAART; Apoptosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400011

Evaluation of recombinant antigens in combination and single formula for diagnosis of feline toxoplasmosis
Provedor de dados: 19
Autores: Abdelbaset, Abdelbaset Eweda; Alhasan, Hend; Salman, Doaa; Karram, Mohamed Hassan; Rushdi, Mahmoud Abd Ellah; Xuan Xuenan; Igarashi, Makoto.
Cats are the only definitive hosts of Toxoplasma gondii and constitute an essential source of infection to all warm blooded animals and humans. Diagnosis of T gondii infection in cats is fundamental for proper management and control of infection in humans and animals. In the current study, we have evaluated the diagnostic performance of tachyzoite lysate antigen (TLA) and different T. gondii recombinant antigens including surface antigen 2 (SAG2), dense granule proteins 2, 6, 7, 15 (GRA2, GRA6, GRA7, GRA15) and microneme 10 protein (MIC10) in immunoglobulin G enzyme linked-immunosorbent assay (IgG ELISA) using cat serum samples, with reference to latex agglutination test (LAT). Remarkably, TLA showed better performance than other recombinant antigens in...
Palavras-chave: ELISA; Recombinant proteins; Serodiagnosis; Toxoplasmosis.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4574

Clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50
Provedor de dados: 19
Autores: Miyama, Takako; Inokuma, Hisashi; Itamoto, Kazuhito; Okuda, Masaru; Verdida, Rodolfo A.; Xuan, Xuenan; 猪熊, 壽; 玄, 学南.
The clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50 was examined in dogs in an area where B. gibsoni infection was endemic. Only 8 among 14 dogs with acute type B. gibsoni infection without a previous history of infection were positive. This high percentage of false-negative results is thought to be a weak point of ELISA as a diagnostic tool. However, 14 other anemic dogs with a confirmed history of B. gibsoni infection were all positive, thus confirming the higher sensitivity of ELISA in detecting a history of infection.
Palavras-chave: Babesia gibsoni; Diagnosis; ELISA.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/925

Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and Neospora caninum-specific antibodies in domestic animals in Turkey
Provedor de dados: 19
Autores: Zhou, Mo; Cao, Shinuo; Sevinc, Ferda; Sevinc, Mutlu; Ceylan, Onur; Liu, Mingming; Wang, Guanbo; Moumouni, Paul Franck Adjou; Jirapattharasate, Charoonluk; Suzuki, Hiroshi; Nishikawa, Yoshifumi; Xuan, Xuenan.
Considering the scarce information on occurrences of Toxoplasma gondii and Neospora caninum in domestic animals from Turkey, the aim of this study was to investigate the seroprevalence of these parasite infections in cattle, horses, sheep, goats and dogs in Turkey. The specific antibodies against T gondii and N. caninum were detected by iELISAs based on the recombinant TgSAG2 or NcSAG1 in a total of 2,039 serum samples from eleven provinces. The seroprevalence of T. gondii infections was 46.3%, 4.0%, 20.0%, 12.9% and 19.8%, that of N. caninum infections was 0.3%, 7.4%, 2.1%, 3.2% and 16.6% in the horses, cattle, sheep, goats and dogs, respectively. These results indicated that T gondii and N. caninum infections are prevalent in Turkish domestic animals.
Palavras-chave: ELISA; Neospora caninum; Toxoplasma gondii; Turkey.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4422

Expression of recombinant dense granule protein 7 of Neospora caninum and evaluation of its diagnostic potential for canine neosporosis
Provedor de dados: 19
Autores: Hara, Olgga A; Liao, Min; Baticados, Waren; Bannai, Hiroshi; Zhang, Guohong; Zhang, Sofa; Lee, Eung-goo; Nishikawa, Yoshifumi; Claveria, Florencia G.; Igarashi, Makoto; Nagasawa, Hideyuki; Xuan, Xuenan.
Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The dense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the present study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and -negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined for the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95 (9.47%) samples from Japan and China, respectively, were...
Palavras-chave: Canine; ELISA; Neospora caninum; NcGRA7.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/995

Identification, Cloning and Characterization of BmP41, a Common Antigenic Protein of Babesia microti
Provedor de dados: 19
Autores: MASATANI, Tatsunori; OOKA, Hideo; TERKAWI, Mohamad A.; CAO, Shinuo; LUO, Yuzi; ASADA, Masahito; HAYASHI, Kei; NISHIKAWA, Yoshifumi; XUAN, Xuenan; 西川, 義文; 玄, 学南.
Babesia microti is a rodent tick-borne blood parasite and the major causative agent of emerging human babesiosis. Here, we identified a candidate of common antigenic protein BmP41 of B. microti by serological screening of cDNA library of human-pathogenic Gray strain with antisera against rodent Munich strain. Immunofluorescent antibody test using mouse anti-recombinant BmP41 (rBmP41) serum revealed that native BmP41 was expressed in each of the developmental stages of B. microti merozoites. An enzyme-linked immunosorbent assay (ELISA) using rBmP41 detected specific antibodies in sera from hamsters infected with B. microti Gray strain and mice infected with B. microti Munich strain. Taken together, BmP41 could be a promising universal serological marker for...
Palavras-chave: Babesia microti; BmP41; Common antigen; ELISA; Immunoscreening.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3917

Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa
Provedor de dados: 19
Autores: NGUYEN, Thu-Thuy; MOTSIRI, Mono Sophie; TAIOE, Moeti Oriel; MTSHALI, Moses Sibusiso; GOTO, Yasuyuki; KAWAZU, Shin-Ichiro; Molifi THEKISOE, Oriel Matlhahane; INOUE, Noboru; 河津, 信一郎; 井上, 昇.
A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8–46.3% and 0–9.1%, respectively. Out of 9 sheep serum samples, 2–4 sera (22.2–44.4%) were positive. The detection performance of crude and recombinant...
Palavras-chave: Animal African trypanosomosis; ELISA; Immunochromatographic test; Serodiagnosis; South Africa.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4034

Seroprevalence of Babesia caballi and Theileria equi in horses and mules  from Northern Thailand
Provedor de dados: 19
Autores: Kamyingkird, K.; Yangtara, S.; Desquesnes, M.; Cao, S.; Adjou Moumouni, P.K.; Jittapalapong, S.; Nimsupan, S.; Terkawi, M.A.; Masatani, T.; Nishikawa, Y.; Igarashi, I.; Xuan, X.
Palavras-chave: Babesia caballi; ELISA; IFAT; PCR; Theileria equi.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4092

Seroprevalence of antibody to NcSAG1 antigen of Neospora caninum in cattle from Western Java, Indonesia
Provedor de dados: 19
Autores: ICHIKAWA-SEKI, Madoka; GUSWANTO, Azirwan; ALLAMANDA, Puttik; MARIAMAH, Euis Siti; WIBOWO, Putut Eko; NISHIKAWA, Yoshifumi.
Neospora caninum can cause fetal abortion and neonatal mortality in cattle, and is a cause of economic concern worldwide. This study aimed to determine the prevalence of Neospora caninum-specific antibodies in cattle from Western Java, Indonesia. Serum samples from 991 cattle from 21 locations were tested for antibodies to N. caninum by using an enzyme-linked immunosorbent assay (ELISA) on the basis of recombinant NcSAG1. The overall seroprevalence was 16.6%, ranging from 0 to 87.5% in the sampled locations. The results of this study indicate latent infection rates of sampled animals were different in each location. Further studies are necessary to elucidate the relationship between N. caninum infection and abortion in cattle, and to identify risk factors...
Palavras-chave: Cattle; ELISA; Indonesia; NcSAG1; Neospora caninum.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4250

Development and evaluation of an enzyme-linked immunosorbent assay using recombinant p23 for the detection of antibodies to Cryptosporidium parvum in cattle
Provedor de dados: 19
Autores: Bannai, Hiroshi; Nishikawa, Yoshifumi; Seo, Jin-yong; Nakamura, Chinatsu; Zhang, Sofa; Kimata, Isao; Takashima, Yasuhiro; Li, Junyou; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南.
An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%)....
Palavras-chave: Cryptosporidium parvum; P23; ELISA; IFAT.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/142

CLONING AND EXPRESSION OF AN ANTIGEN OF BABESIA GIBSONI IN ESCHERICHIA COLI AND ITS USE FOR THE IMMUNODIAGNOSIS OF CANINE BABESIOSIS
Provedor de dados: 19
Autores: Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志.
Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624

Determination of serum isometamidium levels in sheep and goats under field conditions using isometamidium-ELISA
Provedor de dados: 19
Autores: Wesongah, J. O.; Murilla, G. A.; Kibugu, J. K.; Jones, T. W.
Twenty eight sheep and 28 goats were treated with isometamidium chloride (Samorin®, hone Merieux, Lyon, France) at a dose of 1 mg/kg body weight (bw) by intramuscular injection. All the animals were grazed in a tsetse-infested area. They were monitored for anaemia, body weight, anti-trypanosome antibodies and serum isometamidium concentration using the isometamidium enzyme-linked immunosorbent assay (ELISA) for more than 80 days after treatment. Serum isometamidium levels were higher in goats than in sheep throughout the experimental period. Isometamidium was still detectable in sheep and goats for up to 77 and 98 days, respectively, alter treatment (detection limit=0.1 ng/ml). The isometamidium elimination half-lives in sheep and goats were approximately...
Palavras-chave: Isometamidium chloride; ELISA; Sheep; Goats; Trypanosomosis.
Ano: 2000
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/132

CURRENT SERODIAGNOSTIC MEASURES FOR EQUINE BABESIOSIS
Provedor de dados: 19
Autores: Hirata, Haruyuki; Yokoyama, Naoaki; Xuan, Xuenan; Igarashi, Ikuo; 横山, 直明; 玄, 学南; 五十嵐, 郁男.
Palavras-chave: Babesia equi; Babesia caballi; Diagnosis; ELISA; ICT.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/638

Identification and Characterization of a Trypanosoma congolense 46 kDa Protein as a Candidate Serodiagnostic Antigen
Provedor de dados: 19
Autores: ZHOU, Mo; SUGANUMA, Keisuke; RUTTAYAPORN, Ngasaman; NGUYEN, Thu-Thuy; YAMASAKI, Shino; IGARASHI, Ikuo; KAWAZU, Shin-ichiro; SUZUKI, Yasuhiko; INOUE, Noboru; 五十嵐, 郁男; 井上, 昇.
Trypanosoma congolense is a major livestock pathogen in Africa, causing large economic losses with serious effects on animal health. Reliable serodiagnostic tests are therefore urgently needed to control T. congolense infection. In this study, we have identified one T. congolense protein as a new candidate serodiagnostic antigen. The 46.4 kDa protein (TcP46, Gene ID: TcIL3000.0.25950) is expressed 5.36 times higher in metacyclic forms than epimastigote forms. The complete nucleotide sequences of TcP46 contained an open reading frame of 1,218 bp. Southern blot analysis indicated that at least two copies of the TcP46 gene were tandemly-arranged in the T. congolense genome. The recombinant TcP46 (rTcP46) was expressed in Escherichia coli as a glutathione...
Palavras-chave: Diagnosis; ELISA; Nagana; TcP46; Trypanosoma.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3983

Molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of Babesia canis (BcMSA1 and BcSA1)
Provedor de dados: 19
Autores: Zhou, Mo; Cao, Shinuo; Luo, Yuzi; Liu, Mingming; Wang, Guanbo; Moumouni, Paul Franck Adjou; Jirapattharasate, Charoonluk; Iguchi, Aiko; Vudriko, Patrick; Terkawi, Mohamad Alaa; Lowenstein, Mario; Kern, Angela; Nishikawa, Yoshifumi; Suzuki, Hiroshi; Igarashi, Ikuo; Xuan, Xuenan.
Background: Babesia canis is an apicomplexan tick-transmitted hemoprotozoan responsible for causing canine babesiosis in Europe and west Asia. Despite its importance, there is no known rapid diagnostic kit detection of B. canis infection in dogs. The present study identified two novel antigens of B. canis and used the recombinant antigens to establish a rapid, specific and sensitive serodiagnostic technique for detection of B. canis infection. Methods: A complementary DNA (cDNA) expression library was constructed from the mRNA of B. canis and immunoscreened using B. canis-infected dog sera. The cDNAs encoding a merozoite surface antigen and a secreted antigen protein were identified and designated as BcMSA1 and BcSA1, respectively. The recombinant BcMSA1...
Palavras-chave: Babesia canis; Canine babesiosis; BcMSA1; BcSA1; ELISA; Immunochromatographic tests.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4420

Serological detection and epidemiology of Neospora caninum and Cryptosporidium parvum antibodies in cattle in southern Egypt
Provedor de dados: 19
Autores: Fereig, Ragab M.; AbouLaila, Mahmoud Rezk; Mohamed, Samy G. A.; Mahmoud, Hassan Y. A. H.; Ali, Alsagher O.; Ali, Asmaa F.; Hilali, Mosaad; Zaid, Anis; Mohamed, Adel Elsayed Ahmed; Nishikawa, Yoshifumi.
Neospora caninum and Cryptosporidium parvum are intracellular protozoan parasites that are distributed worldwide and of major economical concern in cattle industry. N. caninum can cause abortion storms and high culling rates, whereas C. parvum has zoonotic implications and can cause diarrhea in calves. There are currently no data on the prevalence of neosporosis and cryptosporidiosis in humans or animals in southern Egypt. Prevalence of these two infections was determined in a sample of cattle from two different areas in southern Egypt, Sohag and Qena, using enzyme-linked immunosorbent assay. A total 301 cattle were sampled, of which 18.9% were positive for N. caninum, 35.9% were positive for C parvum and 10.0% were positive for both. Geographical location...
Palavras-chave: Cattle; Cryptosporidium parvum; Neospora caninum; ELISA; Egypt.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4531

Serodiagnosis of ovine toxoplasmosis in Mongolia by an enzyme-linked immunosorbent assay with recombinant Toxoplasma gondii matrix antigen 1
Provedor de dados: 19
Autores: Tumurjav, Buyannemekh; Terkawi, Mohamad Alaa; Zhang, Houshuang; Zhang, Guohong; Jia, Honglin; Goo, Youn-Kyoung; Yamagishi, Junya; Nishikawa, Yoshifumi; Igarashi, Ikuo; Sugimoto, Chihiro; Xuan, Xuenan.
Toxoplasma gondii matrix antigen 1 (TgMAG1), known as the 65-kDa protein, which is abundantly expressed in both bradyzoites and tachyzoites, was evaluated as a candidate for the development of a diagnostic reagent for ovine toxoplasmosis. The TgMAG1 gene was expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST),and the recombinant TgMAG1 (rTgMAG1) was tested in an enzyme-linked immunosorbent assay (ELISA). The ELISA with rTgMAG1 showed a highly specific reaction with sera from mice experimentally infected with T. gondii but not with the closely related Neospora caninum. The antibodies to TgMAG1 were detectable from the acute to the chronic infectious stages in a mouse model. A total of 175 serum samples collected from sheep...
Palavras-chave: ELISA; Mongolia; Sheep; TgMAG1; Toxoplasma gondii.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3018

Diagnostic Methods for the Detection of Cryptosporidium parvum Oocysts in Faeces
Provedor de dados: 19
Autores: Webster, K. A.; Green, J. A.; Dawson, C.; Giles, M.; Catchpole, J.
Palavras-chave: Cryptosporidium parvum; Diagnosis; Immunofluorescence; ELISA; Coproscopical.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/253

Summary Proceedings of the Second Coordinators' Meeting on Peanut Stripe Virus
Provedor de dados: 30
Palavras-chave: Viruses; Groundnuts; Genotypes; Yield losses; ELISA; Peptides; Quarantine; Legumes; Germplasm; HPLC.
Ano: 1989
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4070

Rate of transmission of Indian peanut clump virus to groundnut by mechanical inoculation
Provedor de dados: 30
Autores: Reddy, A.S.
Palavras-chave: Groundnuts; ELISA; Genotypes; Poultry equipment; Application methods; Temperature; Germplasm; Barley; Inorganic acid salts; Potassium.
Ano: 2005
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3224

coordinated research on groundnut of Rosette virus disease
Provedor de dados: 30
Autores: D. McDonald.
Palavras-chave: Groundnuts; Viruses; RNA; Grafting; ELISA; Genotypes; Backcrossing; Breeds (animals); Resistance varieties; Scions.
Ano: 1987
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3144

Working together on groundnut virus diseases
Provedor de dados: 30
Autores: D.V.R.
Palavras-chave: Viruses; Groundnuts; RNA; Genes; ELISA; Genotypes; Transgenics; PCR; Genetic structures.
Ano: 1994
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4178

Working Together on Groundnut Virus Diseases
Provedor de dados: 30
Autores: D.V.R.( editor).
Palavras-chave: Viruses; Groundnuts; RNA; Genes; ELISA; Genotypes; Transgenics; PCR; Genetic structures.
Ano: 1994
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3121

Effect of harvest timing and postharvest storage conditions on aflatoxin contamination in groundnuts harvested from the Wonogiri regency in Indonesia
Provedor de dados: 30
Autores: Rahmianna,A.A.
Palavras-chave: Mycotoxins; Groundnuts; ELISA; Polymers; Retail marketing; Sampling; Storage; Harvesting; Fungi; Quality.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3358

International Arachis Newsletter
Provedor de dados: 30
Palavras-chave: Groundnuts; ELISA; Necrosis; Mutants; Mycotoxins; Genotypes; Breeds (animals); Genetic soil types; Root nodulation; Agronomy.
Ano: 2023
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3965

Summary and Recommendations of the International Workshop on Aflatoxin Contamination of Groundnut
Provedor de dados: 30
Palavras-chave: Mycotoxins; Groundnuts; ELISA; Genotypes; Sampling; Detoxification; Chromatography; Toxins; Fungi; Immunological techniques.
Ano: 1988
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4056

Coordinated Research on Groundnut Rosette Virus Disease
Provedor de dados: 30
Palavras-chave: Groundnuts; Viruses; RNA; Grafting; ELISA; Genotypes; Backcrossing; Breeds (animals); Resistance varieties; Scions.
Ano: 1988
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4054

Bud Necrosis:Bud Necrosis: A Disease of Groundnut Caused by Tomato Spotted Wilt Virus
Provedor de dados: 30
Autores: D.V.R.
Palavras-chave: Necrosis; ELISA; Groundnuts; Sucrose; Amines; Insecticides; Cytoplasmic organelles; Poultry equipment; Viruses; Antibiotics.
Ano: 1990
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4665

Diversidade de Gluconacetobacter diazotrophicus isolada de plantas de cana-de-açúcar cultivadas no Brasil
Provedor de dados: 86
Autores: Perin,Liamara; Baldani,José Ivo; Reis,Veronica Massena.
O objetivo deste trabalho foi avaliar a diversidade da população de Gluconacetobacter diazotrophicus oriunda de cultivares de cana-de-açúcar (Saccharum spp.) de diferentes regiões e bancos de germoplasma. O estudo foi realizado com 123 isolados, obtidos de folhas, colmos e raízes de 80 espécies e híbridos de cana-de-açúcar, procedentes de diferentes países e mantidos em coleções de germoplasma nos Estados da Bahia e Rio de Janeiro. Foram utilizados cinco isolados obtidos de plantas de café (Coffea arabica), dois de abacaxi (Ananas comosus) e um de Pennisetum purpureum e mais 10 estirpes com padrões eletroforéticos distintos, após o uso de enzimas comuns do metabolismo microbiano (MLEE). O agrupamento obtido por meio da técnica de imunoadsorção com enzima...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ELISA; Fixação biológica de nitrogênio; Bactérias endofíticas.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000800006

Anticorpos Para o Vírus da Anemia das Galinhas (CAV) em Matrizes de Corte no Brasil
Provedor de dados: 89
Autores: Brentano,L; Silva,BG; Sayd,S; Flores,SW.
Apesar do diagnóstico da anemia infecciosa e mais evidências recentes de infecção em campo (Brentano &amp;amp; Ottati, 1999), não havia até hoje dados disponíveis da prevalência do vírus da anemia das galinhas (CAV) no Brasil. Uma vez que o conhecimento da prevalência do vírus e da distribuição de anticorpos nos lotes de matrizes é essencial para o delineamento de estratégias de controle da doença, realizamos um levantamento em 5 Estados com produção comercial intensiva de linhas de corte, abrangendo lotes de aves com diferentes idades. De 2.355 soros testados por ELISA, 89% foram positivos, indicando a alta prevalência do CAV no Brasil, em todos os Estados testados. A análise do soro de aves das 6 até as 70 semanas de idade demonstrou que lotes de 6 a 18...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anemia infecciosa das galinhas; Vírus da anemia das galinhas; Anticorpos; ELISA.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2000000200006

Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
Provedor de dados: 89
Autores: Oliveira,GH; Berchieri Jr,A; Montassier,HJ.
This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; ELISA; Enzyme; Salmonella sp; Serology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100008

Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
Provedor de dados: 89
Autores: Oliveira,GH de&lt;A NAME="nt01a"&gt;&lt;/A&gt;; Berchieri Júnior,Â; Montassier,HJ; Fernandes,AC.
This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chickens; Conjugate; ELISA; Salmonella sp; Serology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007

Circulation of Major Respiratory Pathogens in Backyard Poultry and their Association with Clinical Disease and Biosecurity
Provedor de dados: 89
Autores: Batista,IA; Hoepers,PG; Silva,MFB; Nunes,PLF; Diniz,DCA; Freitas,AG; Cossi,MVC; Fonseca,BB.
SUMMARY Raising backyard birds is a common practice in Brazil, mainly in the countryside or suburban areas. However, the level of respiratory pathogens in these animals is unknown. We sampled two hundred chickens from 19 backyard flocks near commercial poultry farms and performed ELISA to Infectious Bronchitis Virus, avian Metapneumovirus, Mycoplasma synoviae and Mycoplasma gallisepticum. We evaluated the association between the predictive ability of ELISA and Hemagglutination-inhibition (HI)by comparing results from eight flocks positive to Mycoplasma gallisepticum on ELISA. Besides, we assessed essential biosecurity measures in the properties (multiple species birds, rodent control, hygienic conditions, and water quality for the bird`s consumption). We...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian Metapneumovirus; ELISA; Hemagglutination-inhibition; Infectious Bronchitis Virus; Mycoplasma.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100324

Development of an enzyme-linked immunosorbent assay for quantification of Salmonella enteritidis-specific antibodies in egg yolk
Provedor de dados: 89
Autores: Tayeb,IT; Nehme,P; Jaber,L; Barbour,EK.
The present study aims at developing an indirect ELISA to quantify yolk antibodies specific to all surface proteins of the invasive Salmonella Enteritidis (SE), which acquired the 1.8, 14.1, and ~ 50 Kb plasmids. An ELISA checkerboard was used in four different experiments to account for the different parameters included in the preliminary ELISA procedure, and consequently to maximize the difference in Optical Density (OD) values between control positive and negative yolk samples. The first experiment aimed at studying the impact of 5% Bovine Serum Albumin (BSA) dissolved in distilled water as a blocking reagent on a 28 µg/well SE antigen-coated plate, while applying the positive and negative control yolk samples to different concentrations of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibodies; Egg yolk; ELISA; Salmonella Enteritidis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400009

Comparative Study of Three Different ELISA to Measure the Antibodies Against Infectious Bronchitis Virus in Vaccinated and Unvaccinated Broilers
Provedor de dados: 89
Autores: Cardoso,TC; Oliveira,C; Da-Silva,SEL; Ferreira,HL; Pinto,AA.
Broilers were spray-vaccinated (n=150) with H120 serotype at one-day-old, challenged after 28 days with M41 IBV serotype and after bled at day 28, 34 and 46 after challenged. The respective sera were tested by the indirect ELISA (I-ELISA), sandwich ELISA (S-ELISA), liquid phase blocking ELISA (LPB-ELISA) and the standard serum neutralization test (SNT). For this purpose, a total of 300 sera samples, 150 from non vaccinated and 150 from vaccinated broilers were titrated by all the serological methods and the correlation coefficients were determined. The correlation values (r) between LPB-ELISA x SNT, S-ELISA x SNT, I-ELISA x SNT found werer = 0.98, r = 0.79, and r = 0.74, respectively. Nevertheless, the r between LPB-ELISA x S-ELISA, LPB-ELISA x I-ELISA and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ELISA; Post vaccinal response; Infectious bronchitis virus; Broilers.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2001000100010

Elisa evaluation of the levels of antibodies against Infectious Bronchitis Virus in laying hens using egg yolk as substrate
Provedor de dados: 89
Autores: Rauber,RH; Flôres,ML; Pereira,CE; Grigulo,M.
The present work was carried out to compare Infectious Bronchitis Virus (IBV) antibody titers in serum and egg yolk samples from laying hens. Sixty paired blood and egg samples were collected from laying hens of two farms. Serum samples were frozen, while egg yolk samples were diluted (1:500) before freezing. Serum and yolk samples were tested for the presence of IBV antibodies by indirect ELISA (commercial kit) and titers were compared by a correlation test (alpha=0.05). There was a high correlation (r=0.62) between the two kinds of samples, which means that titers of IBV antibodies in the egg yolk and in serum samples are quite the same. Considering that blood collection causes deep stress that leads to economic losses, and since eggs are collected daily...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibody; Egg yolk; ELISA; Infectious bronchitis; Serology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200008

A Simple Kit of Plate-Trapped Antigen Enzyme-Linked Immunosorbent Assay for Identification of Plant Viruses
Provedor de dados: 124
Autores: Nascimento,Aline Kelly Queiróz; Lima,José Albersio Araújo; Barbosa,Graziela da Silva.
ABSTRACT Many methods can be used for arriving in a correct virus disease diagnose, and the serological techniques are the most used methods for plant virus identification. The indirect enzyme-linked immunosorbent assay (Indirect-ELISA) or plate-trapped antigen ELISA (PTA-ELISA) has been useful for detection of viruses in a wide range of situations, especially to test a large number of samples in a relatively short period of time. Immune-biological Companies have developed practical kits for direct ELISA or double antibody sandwich (DAS-ELISA), but neither Company has developed kits for PTA-ELISA. As a single universal antibody-conjugate is used for detection of a wide range of plant viruses, the PTA-ELISA technique is more economical, practical and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant Virus Diagnose; ELISA; Comovirus; Cucumovirus; Potyvirus; Sobemovirus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000100216

Aspectos clínicos y de diagnóstico de la gnatostomosis experimental canina
Provedor de dados: 108
Autores: Álvarez-Guerrero,César; Muñoz-Guzmán,Marco Antonio; Alba-Hurtado,Fernando.
En 4 perras infectadas con larvas de Gnathostoma binucleatum se detectaron fases del parásito en pared gástrica. Una presentó un nódulo con gusanos adultos en el interior, 2 presentaron nódulos con larvas y la otra presentó fases juveniles sin nódulos. El periodo de prepatencia en la perra con gusanos adultos fue de 22 semanas y el de patencia de 14 semanas. Se realizó la descripción de la morfología del huevo y del cuadro clínico. En la perra con gusanos adultos se detectó, por ultrasonografía, una masa cavernosa de 57 x 24 mm en la pared del estómago, y por endoscopia, se observó la proyección de esta masa hacia el lumen gástrico. Los niveles de anticuerpos contra antígenos de larvas aumentaron (P &lt; 0.05) a partir del segundo mes pi, el Western blot...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gnathostoma; Gnatostomosis canina; Diagnóstico; Antígenos; ELISA; Perro.
Ano: 2012
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922012000100003

Formulação e avaliação da proteção e resposta humoral de uma vacina de dna contra linfadenite caseosa
Provedor de dados: 195
Autores: Sanches, Simone Camargo; Carvalho, Cleber Eduardo Galvão; Fragoso, Stenio Perdigão; dos Santos, Lenita Ramires; Rosinha, Grácia Maria Soares; Soares, Cleber Oliveira.
O objetivo deste estudo foi desenvolver uma vacina de DNA contra linfadenite caseosa, testá-la em camundongos BALB/c e avaliar a produção de anticorpos específicos e os níveis de proteção conferidos aos animais testados. Para isso, o gene denominado proteína de superfície de membrana (psm), que produz uma proteína potencialmente antigênica, selecionado por técnica de imunovarredura de uma biblioteca de expressão de C. pseudotuberculosis, foi usado como alvo da construção de uma vacina de DNA. Parte desse gene foi amplificada pela PCR e clonada nos plasmídeos pET28a e pcDNA3.1+ para produção de proteína recombinante in vitro e expressão in vivo, respectivamente. Grupos de cinco camundongos da linhagem BALB/c receberam quatro doses do candidato a imunógeno e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Linfadenite Caseosa; Vacina de DNA; Proteína recombinante; ELISA.
Ano: 2015
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/24831

Serologic survey for ophidian paramyxovirus in captive snakes
Provedor de dados: 195
Autores: Paiva, Maria Isabel Sousa; Araújo Junior, João Pessoa; Barraviera, Benedito.
The captivity maintenance of snakes is a challenge for the breeders, especially in diseases control. The most common diseases in serpentarium and private collections are the parasites, gastrointestinal problems and respiratory viruses. The biggest concern is the ophidian paramyxovirosis caused by ophidian paramyxovirus (OPMV), which primarily affects the respiratory system and may also involve the nervous system. Often fatal, it was described in Colubridae, Elapidae, Viperidae, Boidae and Pitonidae. This virus is transmitted by aerosols, respiratory secretions, contaminated utensils, ectoparasites such as ticks and digestive excreta. There is no specific treatment or vaccine available. The diagnostic methods are: virus isolation in cell culture; electron...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snakes; Ophidian paramyxovirus; Infection; ELISA; Prophylaxis serpentes; Paramixovirose ofídica; Infecção; ELISA; Profilaxia.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/31033

Diagnóstico de leishmaniose visceral canina em animais do Centro de Controle de Zoonoses da Bahia
Provedor de dados: 195
Autores: Carvalho, Fábio Santos; Rocha, Josiane Moreira; Wenceslau, Amauri Arias; Oliveira, Haniel Cedraz de; Santos, Ivanildo dos Anjos; Gross, Eduardo.
A Bahia é o estado da região Nordeste com maior número de registros de leishmaniose em humanos.Os cães também podem se infectar e tem importante papel na manutenção da doença no ambiente urbano. Sendo assim, objetivou-se investigar a ocorrência de leishmaniose visceral canina em animais das unidades de Centro de Controle de Zoonoses (CCZ) da Bahia utilizando técnicas sorológicas e moleculares. Foram examinados 100 cães dos CCZ dos municípios Barreiras, Eunápolis, Ilhéus, Itabuna e Feira de Santana. Após exame clínico dos animais coletou-se 10 mL de sangue da veia jugular para avaliação hematológica, extração de DNA e obtenção de soro. O DNA genômico foi extraído utilizando-se fenol:clorofórmio:álcool isoamilico (25:24:1). O diagnóstico sorológico foi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L. infantum; ELISA; PCR; Cães.
Ano: 2015
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/25304

Detección y caracterización molecular del Virus X de la Papa (PVX) en regiones productoras de papa de Colombia
Provedor de dados: 100
Autores: Gil Ramírez,J.F; Cotes Torres,J.M; Marín Montoya,M.
El cultivo de papa (Solanum tuberosum L.) se ve afectado por diferentes virus que reducen su rendimiento y la calidad del tubérculo-semilla. Determinar la distribución y la variabilidad genética de dichos patógenos, es fundamental para los programas de manejo integrado y mejoramiento genético. En este trabajo se evaluó la presencia y la variabilidad molecular de aislados de PVX en cultivos de papa de los departamentos de Antioquia, Cundinamarca, Boyacá y Nariño (Colombia). La evaluación de presencia se realizó mediante pruebas de ELISA, mientras que el análisis de variabilidad incluyó la secuenciación de una región parcial de la cápsida viral en 14 aislamientos y de porciones de los genes codificantes para las proteínas RdRp y Tgb1 para dos aislamientos....
Tipo: Journal article
Palavras-chave: Alphaflexiviridae; ELISA; RT-PCR; Solanum tuberosum; Variabilidad genética.
Ano: 2012
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522012000200001

DETECCIÓN MOLECULAR DE VIRUS EN MATERIAL DE SIEMBRA DE TOMATE DE ÁRBOL EN COLOMBIA
Provedor de dados: 100
Autores: Álvarez,J; Miguel Cotes,J; Marín,M.
La virosis del tomate de árbol en Colombia es causada por un complejo de al menos siete virus, que incluyen miembros de los géneros Potyvirus, Cucumovirus, Polerovirus y Tobamovirus, entre otros. La propagación de este frutal se realiza por semilla sexual, por lo cual es fundamental garantizar su sanidad, especialmente de virus como PVY y PLRV, que han sido informados y pueden ser transmitidos por esta vía. En la investigación se realizaron los análisis filogenéticos de aislados de PVY y PLRV obtenidos a partir de muestras de semilla sexual y plántulas de vivero de tomate de árbol procedentes de diferentes regiones de Colombia. También se desarrollaron técnicas de hibridización molecular con sondas dirigidas al gen de la cápside, como herramientas de...
Tipo: Journal article
Palavras-chave: ELISA; Hibridización molecular; PLRV; PVY; Solanum betaceum.
Ano: 2011
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522011000200003

OPTIMIZACIÓN DE MÉTODOS SEROLÓGICOS PARA LA DETECCIÓN DE Ralstonia solanacearum (SMITH) YABUUCHI
Provedor de dados: 100
Autores: Álvarez,Elba; Iglesia,Aleika; García,A; Blanco,Elizabet.
La marchitez bacteriana causada por Ralstonia solanacearum, afecta a varios cultivos económicamente importantes. El manejo seguro de esta enfermedad depende del diagnóstico preciso y temprano. El objetivo del presente trabajo fue la optimización de métodos serológicos para la detección de R. solanacearum. Se obtuvieron anticuerpos policlonales para ser utilizados en las técnicas serológicas. Se determinó la sensibilidad y especificidad de los métodos. El límite de detección fue desde 10(4) a 10(6) UFC/mL para los extractos de plantas y tubérculos mediante el sistema ultramicroanalítico (SUMA), DAS- ELISA, Dot-blot, aglutinación con partículas látex e inmunofluorescencia. Cuando las muestras fueron previamente incubadas en caldo SMSA, antes de ser...
Tipo: Journal article
Palavras-chave: Marchitez bacteriana; Ralstonia solanacearum; Sistema ultramicroanalítico; ELISA; Dot-blot; Aglutinación con látex; Inmunofluorescencia.
Ano: 2008
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522008000100005

NIVELES DE AFLATOXINA M1 EN LECHE CRUDA Y PASTEURIZADA COMERCIALIZADA EN LA ZONA METROPOLITANA DE GUADALAJARA, MÉXICO
Provedor de dados: 101
Autores: Patricia,Landeros; Noa,M; López,Yolanda; González,Delia G; Noa,Elizabeth; Real,M; Juárez,C; Medina,Miriam S.
Entre los indicadores de calidad higiénico sanitaria de la leche en México, se encuentra el nivel de contaminación con aflatoxina M1 (AFM1), proveniente de animales que han consumido alimento contaminado con aflatoxina B1 (AFB1). Los tratamientos térmicos como la pasteurización, generalmente no disminuyen la concentración de AFM1, por lo que en el presente trabajo se estudió la presencia de AFM1 en leche cruda de vaca de 10 centros de acopio (n=50) y 7 marcas de leche pasteurizada (n=84) comercializadas en la zona metropolitana de Guadalajara, Jalisco, México. La determinación de AFM1 se realizó mediante un juego de reactivos tipo ELISA competitivo- directo (Agraquant® Aflatoxina M1, Romer Labs). El análisis estadístico se llevó a cabo mediante una prueba...
Tipo: Journal article
Palavras-chave: Leche; Aflatoxina M1; ELISA; México.
Ano: 2012
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2012000100006

Detection of a high prevalence of antibodies against Toxoplasma gondii in cattle in Northern and Midwestern Brazil
Provedor de dados: 101
Autores: Barbosa da Silva,Jenevaldo; Nunes de Santana Castro,Gustavo; Nunes dos Santos,Priscilla; Henrique da Fonseca,Adivaldo; Henrique da Silva Lima,Danillo; dos Anjos Bomjardim,Henrique; dos Santos Belo Reis,Alessandra; de Oliveira Soares,Susiane; Diomedes Barbosa,José.
The aim of this study was to determine the prevalence of Toxoplasma gondii in cattle from the Northern and Midwestern regions of Brazil. Serum samples were collected from 1789 animals and tested by indirect enzyme-linked immunosorbent assay (ELISA). The overall prevalence of T. gondii was 83.40% (1492/1789). The prevalence rates of T. gondii-seropositive animals observed in the states of Pará, Tocantins and, Mato Grosso were 87.45%, 87.79% and 73.06%, respectively. The detection of high prevalence rates of T. gondii in cattle deserves special attention because they are the main source of high biological value protein for humans. This finding indicates the need for further studies on the risk that these animals may pose to public health.
Tipo: Journal article
Palavras-chave: Cattle; ELISA; IFAT; Toxoplasma gondii.
Ano: 2015
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000100008

Tendencias en el diagnóstico de la anaplasmosis bovina
Provedor de dados: 101
Autores: González,Belkis Corona; Obregón,Dasiel; Alemán,Yousmel; Alfonso,Pastor; Vega,Ernesto; Díaz,Adrian; Martínez,Siomara.
Anaplasma marginale es una Rickettsia que parasita los eritrocitos maduros del ganado bovino, provocando grandes pérdidas económicas en las regiones tropicales y subtropicales. La enfermedad se caracteriza por marcada anemia hemolítica, disminución del peso, aborto y en ocasiones la muerte. Los animales que logran recuperarse de la fase aguda de la enfermedad permanecen persistentemente infectados y se convierten en reservorio para la transmisión de la misma a los animales sanos, por lo que se hace necesario poder contar con técnicas de diagnóstico más sensibles para ser utilizadas en el movimiento internacional de ganado hacia zonas libres de la enfermedad, que permitan la detección de animales portadores, así como para conocer la prevalencia de la...
Tipo: Journal article
Palavras-chave: Anaplasma marginale; Diagnóstico; ELISA; PCR.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000200001

PRESENCIA DE ANTICUERPOS AL VIRUS DE LA LEUCOSIS BOVINA EN REBAÑOS PERTENECIENTES A LAS PROVINCIAS OCCIDENTALES Y CENTRALES DE CUBA
Provedor de dados: 101
Autores: Delgado,Irma; Alfonso,A; Martínez,Nadia; Abeledo,María Antonia; Rodríguez,Majela; Barrera,Maritza.
El objetivo de este trabajo fue realizar un estudio serológico a varios rebaños bovinos adultos seleccionados por su potencialidad para el comercio. Un total de 597 muestras de suero de bovinos adultos sanos, procedentes de Pinar del Río, La Habana, Ciudad Habana y Villa Clara, fueron analizadas a través de un ELISA indirecto. El 25,9% de las muestras estudiadas resultaron positivas a anticuerpos contra el VLB. Los resultados obtenidos coinciden con reportes anteriores para la región, donde se refiere una alta prevalencia de anticuerpos a este virus. El estudio permitió obtener información sobre la presencia de anticuerpos al VLB en bovinos adultos. Estos resultados sugieren la necesidad de realizar una encuesta para determinar la seroprevalencia en...
Tipo: Journal article
Palavras-chave: Virus leucosis bovina enzoótica; ELISA; Leucosis; Anticuerpos; Bovinos.
Ano: 2009
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000100005

Serological prevalence and risk factors of Borrelia burgdorferi in water buffaloes (Bubalus bubalis, Linnaeus, 1758) on Marajó Island, northern region of Brazil
Provedor de dados: 101
Autores: Barbosa da Silva,Jenevaldo; Baêta,Bruna de A.; Lopes,Cinthia T.A.; Martins Land Manier,Bruna Sampaio; Nunes Santana de Castro,Gustavo; Nunes dos Santos,Priscilla; da Fonseca,Adivaldo Henrique; Diomedes Barbosa,José.
Sera samples were collected from 330 water buffaloes on Marajó Island, state of Pará, Brazil, to assess the presence of antibodies against Borrelia burgdorferi by indirect Enzyme Linked Immunosorbent Assay. Approximately 45% of the animals had antibodies against B. burgdoferi. The prevalence of seropositive buffaloes, 72% (85/118), was statistically higher in the city of Soure than in the other municipalities tested. Murrah breed animals were significantly more seropositive (Prevalence Ratio (PR) = 1.84, p = 0.000) than those of the Mediterranean breed. Among the animals diagnosed positive for tuberculosis, 33% (4/12), were also seropositive for B. burgdoferi. Animals positive for tuberculosis had a significantly lower level of B. burgdorferi...
Tipo: Journal article
Palavras-chave: Spirochetes; Borrelia sp.; ELISA; Buffaloes; Risk factors; Espiroquetas; Borrelia sp.; ELISA; Búfalos; Factores de riesgo.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000300002

Comparación entre un ELISA indirecto y la técnica de aglutinación microscópica para la detección de anticuerpos antileptospirales en caninos
Provedor de dados: 101
Autores: García,Radamés L.; Feraud,Dania; Lugo,Sonia; Machado,Héctor; Abeledo,María A.
Con el objetivo de comparar un ELISA indirecto elaborado a partir de Leptospira biflexa serogrupo Semaranga serovar Patoc, con la técnica de aglutinación microscópica (de su sigla en inglés MAT) en el diagnóstico serológico de la leptospirosis canina, se utilizaron 77 muestras de suero sanguíneo de caninos con sospecha de leptospirosis (Grupo 1) y 84 muestras de animales aparentemente sanos (Grupo 2), los cuales fueron investigados simultáneamente por el ensayo inmunoenzimático y por la técnica de aglutinación microscópica (MAT). Los valores de prevalencia obtenidos por el MAT y el ELISA, así como entre ambos grupos no mostraron diferencias significativas, lo que indica la coincidencia entre ambos métodos y la existencia de una amplia circulación de cepas...
Tipo: Journal article
Palavras-chave: Leptospirosis canina; Diagnóstico serológico; ELISA; MAT.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000200007

Seroprevalencia de Sarcocystis spp. en un hato lechero del municipio de Toca, Colombia
Provedor de dados: 101
Autores: Pulido-Medellín,Martín O; García-Corredor,Diego; Andrade-Becerra,Roy.
Se determinó la seroprevalencia de Sarcocystis spp. en los bovinos de un hato lechero del municipio de Toca, Colombia y su relación con la edad, estado reproductivo y número de partos, utilizando la técnica de ELISA desarrollada por la Fundación Colombiana de Estudios de Parásitos (FUNCEP); la muestra calculada con un nivel de confianza de 95% fue de 100 animales para la realización de la investigación. La prevalencia de Sarcocystis spp. encontrada en este estudio fue del 64%; se observó que el parásito puede presentarse a cualquier edad pero con mayor cantidad de individuos infectados en edades entre los 37 y 48 meses (20.3%). La infección con Sarcocystis spp. puede presentarse en vacas gestantes o vacías sin diferencias entre ellas; sin embargo, se...
Tipo: Journal article
Palavras-chave: Sarcocystis; ELISA; Seroprevalencia; Bovinos; Parasitología.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000300003

Survey of mycotoxins in Southern Brazilian wheat and evaluation of immunoassay methods
Provedor de dados: 63
Autores: Duffeck,Maíra Rodrigues; Tibola,Casiane Salete; Guarienti,Eliana Maria; Del Ponte,Emerson Medeiros.
ABSTRACT One hundred commercial wheat grain samples were collected during the 2015 sea-son across 78 municipalities in the states of Paraná (PR), Rio Grande do Sul (RS), and São Paulo (SP), Brazil. Separate subsamples were analyzed for the concentration of deoxynivalenol (DON), zearalenona (ZEA) and ochratoxin A (OTA) mycotoxins using two methods: UHPLC-MS/MS (reference method) and a commercial enzyme-linked immunosorbent assay (ELISA) (AgraQuant®). The OTA mycotoxin was not found in the samples by both methods. DON and ZEA were detected in 55 % and 39 % of the samples by the reference method, with overall mean levels of 795.2 μg kg−1 and 79.78 μg kg−1, respectively. There was a significant and positive correlation (Spearman rank) between DON and ZEA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; UHPLC; ELISA; Trichothecenes; Ochratoxin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000400343

Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds
Provedor de dados: 63
Autores: Popovic,Tatjana; Milovanovic,Predrag; Aleksic,Goran; Gavrilovic,Veljko; Starovic,Mira; Vasic,Mirjana; Balaž,Jelica.
Halo blight, caused by Pseudomonas savastanoi pv. phaseolicola (Psp), is considered to be an important bacterial disease on common bean (Phaseolus vulgaris L.) in Serbia. Use of pathogen-free seeds is one of the most effective control measures against this disease. The aim of this study was to evaluate a detection method for Psp on untreated common bean seeds (23 genotypes) from commercial crops grown within Serbia. Detection of this pathogen was made by plating onto the modified sucrose peptone (MSP) and Milk Tween (MT) semi-selective mediums from soaked whole common bean seed. Colonies growing on the MSP medium were light yellow, convex and shiny, whereas on the MT medium, they were creamy white, flat and circular. The pathogenicity of the obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MSP; MT; ELISA; Nested-PCR; Halo blight; Pathogenicity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400005

Breakdown of resistance in sweet pepper against Pepper yellow mosaic virus in Brazil
Provedor de dados: 63
Autores: Gioria,Ricardo; Braga,Renato de Souza; Krause-Sakate,Renate; Roullier,Caroline; Rosa,Daniel Dias; Moura,Monika Fecury; Souza-Dias,José Alberto Caram de; Sawazaki,Haiko Enok; Camargo,Luis Eduardo Aranha; Rezende,Jorge Alberto Marques.
Plants of Capsicum annuum cv. Magali R, resistant to Pepper yellow mosaic virus (PepYMV), which showed severe yellow mosaic, leaf malformation and stunting were observed during the 2003/04 growing season in Lins, São Paulo State, Brazil. Potyvirus-like particles observed in leaf sap from infected plants under the electron microscope reacted with an antiserum against PepYMV in PTA-ELISA. In addition to C. annuum cv. Magali R, this potyvirus also infected systemically the resistant C. annuum cv. Rubia R. The nucleotide sequence of part of the CP gene of this potyvirus shared 96-98% identity with that of other PepYMV isolates. The partial nucleotide sequence of the 3' NTR showed 94-96% identity with that of PepYMV. These data indicate that this potyvirus is a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potyvirus; RT-PCR; ELISA; Resistance gene.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200017

Main viruses in sweet cherry plantations of Central-Western Spain
Provedor de dados: 63
Autores: Sánchez,Rodrigo Pérez; Corts,Remedios Morales; Benavides,Pablo García; Sánchez,María Ángeles Gómez.
Sweet cherry trees (Prunus avium L.) are susceptible to a range of diseases, but there have been no studies to date about the viral infection of sweet cherry trees in Spain. To determine the phytosanitary status of Spanish sweet cherry plantations, the incidence and leaf symptoms induced by Prune dwarf (PDV), Prunus necrotic ringspot (PNRSV) and Apple chlorotic leaf spot (ACLSV) viruses were investigated during 2009. Young leaf samples were taken from 350 sweet cherry trees, corresponding to 17 cultivars, and were analysed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). To associate the leaf symptoms with the virus, 50 mature leaves from each infected tree were visually inspected during the summer. The ELISA results revealed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prunus avium; ELISA; Stone fruit; Symptoms.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100083

การปนเปื้อนของสารแอฟลาทอกซิน บี 1 และสารโอคราทอกซิน เอ ในข้าวกล้องและข้าวขาว
Provedor de dados: 106
Autores: Amara Chinaphuti; Suppara Aukkasarakul.
Rice is the stable food of Thai people and also to be the first level among the agricultural commodities for export. In the recent year, most of the consumer give awareness to the safety of food. Moreover, this issue was taken to use as the trade barrier for import-export. This study have been conducted to investigate the possibility of Aflatoxin B1 and Ochratoxin A contamination in brown rice and white rice. Brown rice and white rice samples were collected from 5 markets with 5 samples of each kind per market in Bangkok in June 2009. ELISA technique was employed to use in this study for their rapid test of hundred samples. DOAAflatoxin ELISA test kit was used to detect Aflatoxin B1 whereas Ochratoxin A was detected in rice using ELISA test kit of Neogen,...
Tipo: Collection
Palavras-chave: Brown rice; White rice; Alfratoxin B1; Ochratoxin A; ELISA; ข้าวกล้อง; ข้าวขาว; แอลฟาทอกซิน บี 1; โอคราทอกซิน เอ; อีไลซ่า; การปนเปื้อนเชื้อรา.
Ano: 2009
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5091

Produção de soros policlonais com alta afinidade contra o biotipo 'C' e o biotipo 'S' de Crinipellis perniciosa
Provedor de dados: 102
Autores: Leal Júnior,Gildemberg A; Duarte,Keila M. R; Albuquerque,Paulo S. B; Tavares,Flávio C. A; Figueira,Antonio.
A obtenção de soros policlonais contra fitopatógenos tem por finalidade a elaboração de imunoensaios que permitam identificar e caracterizar os mesmos com rapidez e a custos baixos. Soros policlonais foram produzidos contra extratos das hifas de isolados de Crinipellis perniciosa, agente causal da vassoura-de-bruxa, coletados de cacaueiro (Theobroma cacao) nos estados do Pará e Bahia para identificação e caracterização de isolados de diversas regiões e hospedeiros. O soro denominado de 1 foi produzido a partir do isolado ESJOH-1 (originário do Pará), e o soro 2 foi produzido com o isolado CP-85 (originário da Bahia). Não houve reação cruzada dos soros contra extratos protéicos das espécies Alternaria solani, Marasmius sp., Oudemansiella canarii e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ELISA; Moniliophthora roreri; Variabilidade; Vassoura-de-bruxa; Cacaueiro; Theobroma.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000500004

Polyclonal antibodies to the coat protein of Apple stem grooving virus expressed in Escherichia coli: production and use in immunodiagnosis
Provedor de dados: 102
Autores: Nickel,Osmar; Targon,Maria L.P.N.; Fajardo,Thor V.M.; Machado,Marcos A.; Trivilin,Ana P.
The coat protein gene of Apple stem grooving virus (ASGV) was amplified by RT-PCR, cloned, sequenced and subcloned in the expression vector pMal-c2. This plasmid was used to transform Escherichia coli BL21c+ competent cells. The ASGV coat protein (cp) was expressed as a fusion protein containing a fragment of E. coli maltose binding protein (MBP). Bacterial cells were disrupted by sonication and the ASGVcp/MBP fusion protein was purified by amylose resin affinity chromatography. Polyclonal antibodies from rabbits immunized with the fusion protein gave specific reactions to ASGV from infected apple (Malus domestica) cv. Fuji Irradiada and Chenopodium quinoa at dilutions of up to 1:1,000 and 1:2,000, respectively, in plate trapped ELISA. The ASGVcp/MBP...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Recombinant antigen; Latent virus; ELISA; Malus domestica.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000500017

Expressão em Escherichia coli da proteína capsidial do Watermelon mosaic virus e produção de anti-soro
Provedor de dados: 102
Autores: Barbieri,Manuela R.; Carvalho,Murilo G. de; Zambolim,Eunize M.; Zerbini,F. Murilo.
Um anti-soro policlonal específico para Watermelon mosaic virus (WMV) foi produzido por meio da imunização de coelhos com proteína capsidial purificada, expressa in vitro em células de Escherichia coli. O gene cp foi amplificado via RT-PCR utilizando oligonucleotídeos específicos, a partir de RNA viral extraído de preparações virais concentradas. O fragmento amplificado foi clonado em pBLUESCRIPT KS+ e completamente seqüenciado para confirmação de sua identidade e integridade. Em seguida, o fragmento foi subclonado no vetor de expressão pRSET-A. Plasmídeos recombinantes foram utilizados para a expressão da proteína capsidial em E. coli BL21::DE3. A proteína foi purificada por meio de cromatografia de afinidade em coluna de Ni+-NTA, a partir de proteínas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cucurbitáceas; ELISA; Imunodifusão; Western blot.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000200017

Detection and coat protein gene characterization of an isolate of Grapevine virus B from corky bark-affected grapevines in Southern Brazil
Provedor de dados: 102
Autores: NICKEL,OSMAR; FAJARDO,THOR V. M.; ARAGÃO,FRANCISCO J. L.; CHAGAS,CÉSAR M.; KUHN,GILMAR B.
An isolate of Grapevine virus B (GVB), obtained by indexing Vitis labrusca and V. vinifera grapevines on the indicator LN33, was transmitted mechanically to several Nicotiana species. The virus was partially purified from N. cavicola and the coat protein estimated at 23 kDa by SDS-PAGE. In negatively stained leaf extracts of experimentally inoculated N. cavicola and N. occidentalis, flexuous particles with cross banding were observed, predominantly measuring 750-770 x 12 nm, with a modal length of 760 nm. Decoration indicated a clear, positive reaction against AS-GVB. In DAS-ELISA, GVB was detected in N. cavicola and grapevine extracts, and Western blots showed homologous and cross reaction of GVB and GVA antisera with GVB coat protein. Using specific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GVB; Vitis spp.; Vitivirus; Electron microscopy; ELISA; Western blot; GVA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300007

COINFECCIÓN NATURAL DE VIRUS DE ARN EN CULTIVOS DE PAPA (Solanum tuberosum subsp. Andigena) EN ANTIOQUIA (COLOMBIA)
Provedor de dados: 37
Autores: GALLO GARCÍA,Yuliana; SIERRA MEJIA,Andrea; DONAIRE SEGARRA,Livia; ARANDA,Miguel A.; GUTIERREZ,Pablo Andrés; MARÍN MONTOYA,Mauricio.
RESUMEN Las enfermedades virales son uno de los principales problemas fitopatológicos de la papa. Con el fin de determinar los virus más prevalentes en cultivos de papa var. Diacol Capiro en el oriente Antioqueño (Colombia), se evaluó mediante RT-qPCR la presencia de diez virus de ARN (PVY, PVA, PVV, TaLMV, PVS, PLRV, PYVV, PVX, ToRSV y PMTV) en 36 muestras de tejido foliar. Los resultados indicaron la ocurrencia de cinco de los diez virus evaluados, con niveles de prevalencia de 88,9 %, 75 %, 75 %, 41,7 % y 25 % para PVY, PVX, PYVV, PLRV y PVS, respectivamente. Con fines comparativos, cuatro virus también se evaluaron mediante ELISA, siendo detectados PVS (80,5 %), PVY (55 %) y PLRV (5,5 %); mientras que PVX no fue encontrado con esta prueba. La...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ELISA; RT-qPCR; Secuenciación de nueva generación (NGS); Solanaceae; Virus de ARN.
Ano: 2019
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000300546

CARACTERIZACIÓN SEROLÓGICA Y MOLECULAR DE POTYVIRUS ASOCIADOS A LA VIROSIS DEL TOMATE DE ÁRBOL EN ANTIOQUIA (COLOMBIA)
Provedor de dados: 37
Autores: AYALA VÁSQUEZ,MARILUZ; GONZÁLEZ JAIMES,PAOLA; GUTIÉRREZ,PABLO ANDRÉS; COTES,JOSÉ MIGUEL; MARÍN MONTOYA,MAURICIO.
El tomate de árbol (Solanum betaceum) es una fruta andina altamente nutritiva, con propiedades organolépticas únicas para el procesamiento industrial y el mercado internacional. En Antioquia, Colombia, este cultivo presenta diferentes problemas fitosanitarios, siendo especialmente limitante la Virosis, causada por un complejo viral del que hacen parte entre otras, especies del género Potyvirus. En este trabajo se evaluaron mediante pruebas de ELISA los niveles de incidencia de este grupo viral en cultivos de siete zonas del departamento de Antioquia y se determinó la identidad taxonómica de dos de los potyvirus asociados al cultivo. Las pruebas serológicas muestran la presencia de potyvirus en seis de las siete zonas evaluadas, alcanzando niveles...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ELISA; Potyvirus; PVY; RT-PCR; Tamarillo.
Ano: 2010
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2010000300011

OBTENCIÓN DE ANTICUERPOS ESPECÍFICOS PARA LA DETECCIÓN DEL Tamarillo leaf malformation virus (TALMV) EN TOMATE DE ÁRBOL
Provedor de dados: 37
Autores: GALLO GARCÍA,YULIANA; MARÍN MONTOYA,MAURICIO; GUTIÉRREZ,PABLO ANDRÉS.
En Colombia el rendimiento del cultivo de tomate de árbol se ha visto seriamente afectado por la expansión de una enfermedad conocida como virosis de tomate de árbol. Esta patología se registró inicialmente en 1991 en el norte de Antioquia y su expansión ha alcanzado todas las regiones cultivadoras de este frutal en el país. Trabajos recientes han detectado la presencia de por lo menos dos especies del género Potyvirus (Potyviridae) asociadas a esta enfermedad en los cultivos de tomate de árbol de Antioquia: Potato virus Y (PVY) y Tamarillo leaf malformation virus (TaLMV, especie propuesta). Con el fin de reducir la diseminación de estos patógenos virales en el país, es necesario contar con herramientas de diagnóstico que permitan la certificación del...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticuerpos; ELISA; Potyvirus; TaLMV; Tomate de árbol.
Ano: 2011
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2011000200011

Antígeno excreción-secreción de Fasciola hepatica: ultrafiltración y aplicación en inmunodiagnóstico
Provedor de dados: 38
Autores: Colmenares,Cecilia; Méndez,Liber; Díaz-Bello,Zoraida; Alarcón de Noya,Belkisyolé.
La baja sensibilidad de la coprología en el diagnóstico de la fasciolosis humana ha motivado el desarrollo del inmunodiagnóstico. El antígeno de excreción-secreción de adultos de Fasciola hepatica (AFhES) en ELISA es adecuado para el screening, aunque sobrestima la prevalencia; cuando se utiliza en Western blot (WB) no muestra un buen reconocimiento de sus componentes. Para lograr una mejor preparación, se ultrafiltró el antígeno a través de membranas YM de 10, 30 y 50 kDa. Los retenidos (R) se usaron en ELISA y WB. La mayor discriminación entre positivos y negativos en ELISA y la mejor resolución en el reconocimiento al antígeno en WB, se logró con la fracción R50. Se destacan las moléculas de 9, 14, 65 kDa y la región alrededor de 27 kDa, detectadas con...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fasciolosis; Antígeno; Ultrafiltración; Inmunodiagnóstico; ELISA; Western blot.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572007000200010

Cuantificación de trazas de soja con kits de ELISA en galletitas y productos extrudidos
Provedor de dados: 38
Autores: Binaghi,Maria Julieta; Greco,Carola Beatriz; Martín,María Eugenia; Drago,Silvina Rosa; Ronayne de Ferrer,Patricia Ana; López,Laura Beatriz.
Se evaluó la detección de trazas de soja en sistemas modelo (SM) de galletitas y extrudidos que contenían 5000, 250, 100, 50, 25, 10, 5 y 0 ppm de concentrado de proteína de soja (CPS). Se analizaron, además, dos lotes diferentes de ocho clases de galletitas comerciales y dos tipos de extrudidos que declaraban la presencia de soja a través de la frase &amp;#8220;Contiene ...&amp;#8221; o a través de frases de advertencia. La determinación de proteínas de soja se realizó utilizando tres kits ELISA (R-Biopharm, Neogen-Veratox y Romer). Solo el kit de R-Biopharm permitió la cuantificación de soja a partir de 25 ppm de CPS en galletitas y a partir de 5 ppm de CPS en productos extrudidos. Los otros dos kits resultaron menos sensibles. De acuerdo con los...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alergenos; Soja; Galletitas; Productos extrudidos; ELISA.
Ano: 2017
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572017000400011

DETECCIÓN DEL VIRUS DEL MOSAICO DE LA ALFALFA EN REGIONES PRODUCTORAS DE FREJOL (Phaseolus vulgaris L.) EN CHILE
Provedor de dados: 113
Autores: Sepúlveda R.,Paulina; Morales,Francisco; Castaño,Mauricio.
En los últimos años se han observado plantas de frejol (Phaseolus vulgaris) con síntomas característicos de una enfermedad viral. Durante las temporadas 1993/1994 y 1995/1996 se realizaron prospecciones de campos de frejol en la zona productora (V a la VIII Región) de Chile. Se colectaron un total de 173 muestras de plantas afectadas por diferentes síntomas de posible etiología viral. Las plantas fueron sometidas a la prueba de ELISA con un antisuero para el Virus del Mosaico de la Alfalfa (AMV). Se determinó que era el AMV, el cual estaba distribuido en el área productora de frejol de Chile, pero con una frecuencia de detección relativamente baja, del 2 y 4% respectivamente, para las temporadas mencionadas. El procedimiento descrito junto con la...
Tipo: Journal article
Palavras-chave: AMV; Aislamiento de virus; ELISA.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072001000300012

Identificación e Incidencia de Virus en Pimiento en la Zona Centro Norte de Chile y su Asociación con Vectores
Provedor de dados: 113
Autores: Sepúlveda R,Paulina; Larraín S,Patricia; Quiroz E,Carlos; Rebufel A,Patricia; Graña S,Fernando.
Durante las temporadas de 2001-2002 y 2002-2003 se realizó una prospección de virus en pimientos (Capsicum annuum L.) en diversas localidades de la Región de Coquimbo (29,5-32° lat. Sur). La determinación de virus se realizó mediante la prueba DAS- ELISA. La importancia relativa de los virus encontrados para ambas temporadas fueron: Virus del mosaico del pepino (CMV) 23,3%; Virus del bronceado del tomate (TSWV) 20,8%; Virus del mosaico de la alfalfa (AMV) 14,8%;Virus Y de la papa (PVY) 14,5% e Impatients necrotic spot virus (INSV) 3,1% (primera identificación de este virus en pimiento en Chile); Virus del mosaico del tomate (ToMV) 2,2% y Virus del mosaico del tabaco (TMV) 4,9%. Los resultados indicaron que al menos el 30% de las muestras estaba infectada...
Tipo: Journal article
Palavras-chave: Virus en pimiento; Insectos vectores; Tomato spotted wilt virus; Cucumber mosaic virus; Alfalfa mosaic virus; Potato virus Y; ELISA.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072005000300001

Development of an indirect ELISA assay to evaluation of the adaptive immune response of pacu (Piaractus mesopotamicus)
Provedor de dados: 42
Autores: FARIAS,THAIS H.V.; SILVA,KETHERSON R.; MARIGUELA,VIVIANE C.; MONTASSIER,HÉLIO J.; PILARSKI,FABIANA.
ABSTRACT The pacu is one of the most important species for Brazilian fish farming and is considered emerging in the global aquaculture. Despite its importance, no effective tool for evaluation of the adaptive immune response of this species has been developed. Therefore, this study aimed the development and standardization of indirect ELISA for the measurement of pacu antigen-specific antibodies using polyclonal rabbit anti-pacu IgM used as detector antibody. For this purpose was isolated and purificated of pacu IgM using mannose-binding protein affinity chromatography and produced specific polyclonal antibodies against heavy and light chains pacu IgM, that showed a molecular weight of 72 kDa and 26 kDa, respectively. Polyclonal antibodies obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ELISA; Immunoglobulin; Pacu`s immune system; Polyclonal antisera.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703327

Avaliação de um controle estratégico da artrite encefalite caprina em rebanho caprino leiteiro
Provedor de dados: 45
Autores: Rodrigues,A.S.; Pinheiro,R.R.; Brito,R.L.L.; Andrioli,A.; Oliveira,E.L.; Sider,L.H.; Santos,V.W.; Oliveira,L.S.; Dias,R.P.; Gouveia,A.M.G.; Teixeira,M.F.S.
RESUMO O objetivo deste trabalho foi avaliar a utilização periódica de testes de diagnóstico mais sensíveis aliados às práticas de manejo, visando ao controle eficaz da artrite encefalite caprina (CAE). Foram realizadas oito coletas de sangue em matrizes e reprodutores. Da primeira à sétima análise, as coletas foram quadrimestrais, utilizando-se os testes de imunodifusão em gel de agarose (IDGA), ensaio imunoenzimático indireto (ELISA-i) e Western Blot (WB). A oitava coleta aconteceu seis meses após a sétima, utilizando-se o WB e a reação em cadeia de polimerase (PCR). A prevalência da CAE foi de 6,8%, 14,9% e 39,2% no IDGA, ELISA-i e WB, respectivamente. Na última análise, foram detectados 0,9% de animais positivos pelo WB e 10,8% pela PCR. Apesar de não...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ELISA; IDGA; Lentivírus de pequenos ruminantes; Western Blot.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100139

“Cell ELISA” como ferramenta auxiliar no controle da adenite equina
Provedor de dados: 45
Autores: Ribas,L.M.; Rosa,M.C.; Nogueira,C.E.W.; Finger,I.S.; Cunha,R.C.; Leite,F.P.L.
RESUMO Este trabalho relata o desenvolvimento e a avaliação de um ensaio imunoenzimático (ELISA) como ferramenta auxiliar no controle da adenite equina. Foi avaliada a presença de anticorpos anti-Streptococcus equi subsp. equi em equinos com doença clínica de garrotilho, portadores assintomáticos e potros vacinados. Equinos doentes demonstraram absorbâncias médias superiores (P&lt;0,05) às médias observadas nas demais categorias examinadas. Equinos portadores assintomáticos apresentaram valores médios de absorbância superiores (P&lt;0,05) aos animais com cultura negativa. Logo após a vacinação, potros apresentaram elevação nos níveis de anticorpos, seguida de um decréscimo nos níveis 90 dias após a segunda vacinação. O “Cell ELISA” foi eficiente para a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Garrotilho; Streptococcus equi; ELISA; Portador assintomático.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100020

Efficiency of inactive vaccines against contagious agalactia in Brazil
Provedor de dados: 45
Autores: Campos,A.C.; Azevedo,E.O.; Alcântara,M.D.B.; Silva,R.B.S.; Cordeiro,A.A.; Mamede,A.G.; Melo,M.A.; Rosendo Nascimento,E.; Castro,R.S.
This paper aims to evaluate the efficiency of three inactive vaccines against contagious agalactia prepared with samples of Mycoplasma agalactiae isolated in Brazil and different adjuvants. Vaccine 1 adsorbed with aluminum hydroxide was administered in 23 goats (Gc1) and 13 sheep (Gov1); vaccine 2 containing Montanide IMS-2215-VG was administered in 22 goats (Gc2) and 12 sheep (Gov2) and vaccine 3, containing Montanide Gel-01 was administered in 22 goats (Gc3) and 12 sheep (Gov3). All animals were negative for Ma at indirect ELISA and received two doses of 2mL each, subcutaneously, within a 21 day interval. Five animals from each species were used as control. Seventy-five days after the booster, four animals from each vaccinated group and two from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma agalactiae; Goats; Sheep; Immunization; ELISA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500018

Produção de antígeno e separação da proteína p28 por microfiltragem seriada para sorodiagnóstico da artrite encefalite caprina por ensaio imunoenzimático
Provedor de dados: 45
Autores: Alves,L.A.O.; Teixeira,M.F.S.; Pinheiro,A.A.; Pinheiro,R.R.; Dias,R.P.; Brito,R.L.L.; Lopes Júnior,C.A.F.; Bezerra Júnior,R.Q.; Azevedo,D.A.A.
Este estudo teve como objetivo produzir um antígeno (Ag) a partir de cultura de células de membrana sinovial caprina (MSC) infectadas com o vírus de artrite encefalite caprina (CAEV), pela técnica de microfiltração seriada, substituindo a ultracentrifugação em colchão de sacarose (UCCS) para utilização em ELISA indireto (ELISA-i). Amostras de 188 soros caprinos, que previamente foram testados pelo Western blot (WB) com Ag UCCS, foram submetidas à análise pelo ELISA-i com o novo antígeno produzido, que mostrou concordância de 92% em relação ao antígeno UCCS. A sensibilidade e a especificidade do ELISA em relação ao WB foram de 95,6% e 88,5%, respectivamente. A nova técnica, criada a partir de microfiltrações, mostrou-se efetiva e de baixo custo para o...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caprino; CAEV; ELISA; Lentivírus de pequenos ruminantes; Western blot.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400021

Diagnóstico do complexo teníase-cisticercose bovina em São João Evangelista, Minas Gerais, Brasil
Provedor de dados: 45
Autores: Garro,F.L.; Santos,T.M.; Assis,D.C.S.; Heneine,L.G.D.; Ornellas,C.B.D.; Pinto,P.S.A.; Santos,W.L.M.
Com o objetivo de diagnosticar a situação do complexo teníase-cisticercose bovina em Minas Gerais, Brasil, foi selecionado o município de São João Evangelista, onde foram coletadas amostras de sangue de 339 bovinos em 15 propriedades rurais, sorteadas aleatoriamente. Em cada propriedade, foi aplicado um questionário socioeconômico para a análise de fatores que favorecem a manutenção do complexo teníase-cisticercose bovina. Foi realizado também o diagnóstico de teníase humana por meio de exame coproparasitológico dos habitantes das propriedades. Encontrou-se a prevalência de 4,1% para cisticercose bovina e a frequência de 2,94% para teníase humana. Entre os fatores de risco para a manutenção do complexo teníase-cisticercose analisados, foi observada uma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Cisticercose; ELISA; Immunoblot; Teníase.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000401063

Detecção de resíduos de enrofloxacina por ensaio imunoenzimático e cromatografia líquida acoplada à espectrometria de massas em ovos comerciais de galinha após tratamento
Provedor de dados: 45
Autores: Gouvêa,R.; Santos,F.F.; Machado,L.S.; Panzenhagen,P.H.N.; Aquino,M.H.C.; Nascimento,E. Rosendo do; Ribeiro,C.; Pereira,V.L.A.
A enrofloxacina é um dos antimicrobianos mais utilizados na avicultura industrial, e a deposição de resíduos em produtos avícolas, como os ovos, são de grande importância para a saúde pública. Na legislação brasileira não existe padronização do período de carência para o seu uso na produção avícola e não há Limite Máximo de Resíduo (LMR) fixado para enrofloxacina em ovos. Neste estudo, foi utilizado o kit de ELISA comercial (Bioo Scientific(r)) e a LC-MS/MS na pesquisa de enrofloxacina em ovos de 30 galinhas tratadas previamente via água de bebida, com 10mg/kg de enrofloxacina, durante cinco dias. Seis ovos foram coletados diariamente e analisados durante o tratamento e após a sua suspensão, durante 15 dias. A deposição de resíduos obteve níveis máximos no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enrofloxacina; Resíduos; Ovos; LC-MS/MS; ELISA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601917

Afinidades antigênicas de amostras de campo do vírus da bronquite infecciosa das galinhas com a amostra Massachusetts M41
Provedor de dados: 45
Autores: Souza,M.B.; Martins,N.R.S.; Resende,J.S.
Com o objetivo de avaliar as afinidades antigênicas entre 14 amostras de vírus da bronquite infecciosa das galinhas (VBIG) isoladas de casos clínicos ocorridos entre 1972 e 1989 no Estado de Minas Gerais, sua reatividade frente a dois anticorpos monoclonais (AcMs) específicos contra a glicoproteína S1 do sorotipo Massachusetts de VBIG foi examinada em ELISA. As 14 amostras de campo estudadas foram agrupadas, de acordo com o relacionamento antigênico aos AcMs, em relacionadas (três amostras) e não relacionadas (onze amostras) à amostra M41 do sorotipo Massachusetts. As amostras de campo não reconhecidas, considerando a alta especificidade dos AcMs à amostra M41, compõem uma diversidade que pode variar de integrantes do sorotipo Massachusetts de origem...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galinha; Vírus da bronquite infecciosa; M41; Isolado de campo; ELISA.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000200002

Utilização de um teste de ELISA polivalente para detecção de anticorpos contra Actinobacillus pleuropneumoniae (App)
Provedor de dados: 45
Autores: Kich,J.D.; Piffer,I.A.; Guidoni,A.L.; Barcellos,D.E.S.N.; Klein,C.S.
Um teste de ELISA polivalente baseado em lipopolissacarídeos de cadeia longa (LPS - CL) de Actinobacillus pleuropneumoniae (App) sorotipos 3, 5 e 7 foi avaliado testando-se amostras do soro de leitões e matrizes provenientes de 10 rebanhos positivos e de 10 rebanhos negativos. Foram classificados como positivos aqueles rebanhos com isolamento prévio do App sorotipos 3, 5 ou 7 e rebanhos negativos aqueles submetidos ao controle veterinário, sem notificação de sintomas clínicos, sem lesões de pleuropneumonia suína e sem isolamento do agente. Todos os rebanhos positivos apresentaram sorologia positiva e as matrizes apresentaram maior número de soroconversores (P&lt;0,05) do que os leitões. Entre os rebanhos negativos quatro apresentaram sorologia negativa,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suínos; Actinobacillus pleuropneumoniae; Pleuropneumonia; ELISA.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000500002

Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein
Provedor de dados: 45
Autores: Cândido,A.L.; Resende,M.; Bessa,L.R.G.; Leite,R.C.
A genetic vaccine consisting of the bovine herpesvirus-1.2a (BHV-1.2a) glycoprotein D (gD) gene under the control of the cytomegalovirus immediate-early promoter/enhancer was generated and administered to sheep intramuscularly in the neck. All animals developed serum antibodies which recognized the homologous antigen (BHV-1.2a strain BH-83) and also exhibited cross-reactivity against the heterologous antigen (BHV-5 strain EVI-190). Three intramuscularly injections were given but serological responses were not improved after the second inoculation. Specific antibodies were detected against BHV-1.2a until at least 12 months after the first inoculation. However, the capacity to induce antibodies against BHV-5 was lower and of shorter duration than to BHV-1.2a.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine herpesvirus-1.2a; Glycoprotein D gene; DNA vaccine; ELISA.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300002

Frequency of canine heartworm disease (D. immitis) in some regions of São Paulo State using a circulating antigen indicator test
Provedor de dados: 45
Autores: Souza,N.F.; Larsson,M.H.M.A.
The frequency of canine heartworm disease was determined in 310 dogs (158 males and 152 females) from São Paulo city (154) and several towns along the São Paulo state coast (156). The survey was carried out through the Knott's technique (direct presence of microfilariae in the blood) and ELISA for soluble circulating antigen detection. Twenty five (8.0%) samples were positive to D. immitis using both methods being 6 dogs (3.9%) from the city and 19 (12.2%) from the coastal area. Nineteen dogs (6.1%) were positive to the Knott's technique (4 from the city and 15 from the coastal area) and 12 dogs (3.9%) to the ELISA (4 from the city and 8 from the coastal area); six dogs (1.9%) were positive for both methods being two from the city and four from the coastal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Canine heartworm disease; Dirofilaria immitis; ELISA; Frequency.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000300009

ELISA indireto para detecção de IgG antivírus da doença de Newcastle em soro de codorna
Provedor de dados: 45
Autores: Oliveira,D.D.; Folgueras-Flatschart,A.V.; Flatschart,R.B.; Resende,J.S.; Abreu,J.T.; Martins,N.R.S.
An indirect ELISA for the detection of japanese quail IgG specific to Newcastle disease virus (NDV) was developed. The secondary anti-quail IgG was produced in Balb/c mice, by inoculating Freund's complete adjuvant emulsified japanese quail-IgG extract. The purification of IgG was achieved using the caprilic acid method. The ELISA was compared to the haemagglutination-inhibition (HI) test for antibodies to NDV. ELISA cut-off point was established through TG-ROC analysis. Total correlation was observed between the ELISA and the HI, being the ELISA efficient in the identification of positive and negative sera, with high sensitivity and specificity (100%). These results validate the use of the indirect ELISA as an alternative for the detection of NDV-specific...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Codorna japonesa; ELISA; IgG; Doença de Newcastle.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500040

Infecção por Salmonella enterica em suínos criados em um sistema integrado de produção do sul do Brasil
Provedor de dados: 45
Autores: Silva,L.E.; Gotardi,C.P.; Vizzotto,R.; Kich,J.D.; Cardoso,M.R.I.
Avaliou-se a difusão da infecção em um rebanho com prévio isolamento de Salmonella sp, em que leitões, individualmente identificados, foram amostrados para excreção fecal de Salmonella sp e sorologia do nascimento ao abate. Da mesma forma, amostras de ração e suabes do ambiente foram coletados durante o estudo para pesquisa de Salmonella sp A pesquisa de anticorpos foi realizada pela utilização de ELISA-LPS de Salmonella Typhimurium. Os leitões foram negativos na análise bacteriológica e na sorologia até a fase de creche, tornando-se positivos para Salmonella sp no início da terminação. Nessa amostragem, 28,6% dos animais foram soropositivos e 75% estavam excretando Salmonella sp nas fezes. Ao abate, a percentagem de animais soropositivos (76,9%) aumentou,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suíno; Estudo longitudinal; Salmonella; ELISA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400001

Antígenos de larva de Taenia solium em ELISA para diagnóstico da cisticercose bovina
Provedor de dados: 45
Autores: Monteiro,L.L.; Pinto,P.S.A.; Salcedo,J.H.P.; Araújo,J.V.; Santos,W.L.M.; Cecon,P.R.
Foram avaliados alguns parâmetros inerentes ao ELISA, por meio de ensaios de reatividade de soros-controle positivos e negativos para a cisticercose bovina com relação a três tipos de antígenos de larva de Taenia solium: total, de escólex e de membrana. As concentrações de antígeno de 0,25; 0,5; 1; 2 e 4µg por orifício, e as diluições de soro de 1:25, 1:50, 1:100 e 1:200, foram os parâmetros que menos influenciaram no desempenho do teste. A substância bloqueadora, o leite desnatado e as diluições de conjugado, 1:1.250, 1:2.500 e 1:5.000, representaram os melhores indicadores de desempenho do teste. Concluiu-se que essa combinação de critérios deve ser considerada no diagnóstico da cisticercose bovina, em atividades de rotina ou de padronização do referido...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cisticercose bovina; ELISA; Antígeno; Taenia solium.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000100004

Anemia infecciosa eqüina: prevalência em eqüídeos de serviço em Minas Gerais
Provedor de dados: 45
Autores: Almeida,V.M.A.; Gonçalves,V.S.P.; Martins,M.F.; Haddad,J.P.A.; Dias,R A.; Leite,R.C.; Reis,J.K.P.
Estimaram-se, no estado de Minas Gerais, a prevalência e a distribuição espacial da anemia infecciosa eqüina (AIE) em propriedades com eqüídeos de serviço. As amostras de sangue, de 6540 eqüídeos de 1940 rebanhos foram coletadas no período de setembro de 2003 a março de 2004, nos 853 municípios do estado. Utilizaram-se dois testes de laboratório em seqüência: ELISA, usando-se antígeno recombinante gp90, e imunodifusão em gel de ágar (IDGA). As prevalências foram de 5,3% [IC=4,3 a 6,3%] para rebanhos e de 3,1% [IC=2,2 a 3,9%] para animais. O estado de Minas Gerais foi considerado área endêmica para AIE. As mais altas prevalências para rebanhos e para animais foram encontradas na região Norte/Noroeste, seguida pela região Vale do Mucuri/Jequitinhonha.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eqüídeos; Anemia infecciosa; ELISA; IDGA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000200001

Ocorrência do vírus da imunodeficiência felina e do vírus da leucemia felina em gatos domésticos mantidos em abrigos no município de Belo Horizonte
Provedor de dados: 45
Autores: Teixeira,B.M.; Rajão,D.S.; Haddad,J.P.A.; Leite,R.C.; Reis,J.K.P.
Investigou-se a ocorrência da infecção pelo vírus da imunodeficiência felina (FIV) e pelo vírus da leucemia felina (FeLV) em gatos domésticos, provenientes de dois abrigos, no município de Belo Horizonte. Amostras de sangue de 145 animais foram coletadas e testadas para detecção do FIV pela reação em cadeia da polimerase (PCR). Destas amostras, 40 foram testadas para o antígeno p26 de FeLV por meio de ELISA. Observaram-se duas fêmeas (1,4%) e quatro machos (2,8%) positivos para FIV e nove fêmeas (22,5%) e quatro machos (10,0%) positivos para FeLV.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gato; Vírus da imunodeficiência felina; Vírus da leucemia felina; PCR; ELISA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000400019

Avaliação de testes de ELISA para o diagnóstico sorológico de infecções pelos sorotipos 3, 5 e 7 de Actinobacillus pleuropneumoniae em suínos
Provedor de dados: 45
Autores: Machado,H.G.; Piffer,I.A.; Guidoni,A.L.; Klein,C.; Gil-Turnes,C.
Compararam-se dois antígenos no teste de ELISA para o diagnóstico sorológico dos sorotipos 3, 5 e 7 de A. pleuropneumoniae prevalentes no Brasil. Um compunha-se da fase aquosa da extração fenólica de suspensão bacteriana (FAF) e o outro de lipopolissacarídeos de cadeia longa (LPS-CL). Com esses antígenos foram padronizados ELISAs monovalente e polivalente para os sorotipos prevalentes no Brasil. Com os resultados dos testes de um conjunto de amostras de soro de suínos livres de infecção para A. pleuropneumoniae e um conjunto de amostras de soro obtidas de leitões inoculados com os sorotipos citados e que apresentaram soroconversão, determinaram-se as equações discriminantes para os conjuntos de soros e compararam-se os testes quanto à distância...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suíno; Actinobacillus pleuropneumoniae; Pleuropneumonia; ELISA.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000500001

Padronização de três ELISAs polivalentes com lipopolissacarídeos de cadeia longa dos sorotipos 1 e 5, 2, 3 e 7 ou 10 e 12 de Actinobacillus pleuropneumoniae
Provedor de dados: 45
Autores: Kuchiishi,S.S.; Carvalho,L.F.O.S.; Piffer,I.A.; Kich,J.D.; Ramenzoni,M.L.F.
Três ELISAs polivalentes baseados em lipopolissacarídeos de cadeia longa (LPS-CL) foram estabelecidos para detectar anticorpos para todos os sorotipos prevalentes de Actinobacillus pleuropneumoniae. Foram testadas amostras provenientes do banco de soros de suínos experimentalmente inoculados com todos os sorotipos de A. pleuropneumoniae. Os ELISAs foram sensíveis à detecção de anticorpos contra todos os LPS-CL. Foram observadas reações cruzadas no ELISA polivalente produzido com os sorotipos 1 e 5, com anti-soros específicos para os sorotipos 9 e 11, pois os sorotipos 1, 9 e 11 apresentaram antígenos somáticos comuns. No polivalente com os sorotipos 2, 3 e 7, observaram-se reações com anti-soros dos sorotipos 4, 6 e 8, devido à presença de antígenos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ELISA; Pleuropneumonia; Actinobacillus pleuropneumoniae; Sorologia; LPS.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200016

Use of an ELISA system for detection of equine herpesvirus 1 (EHV-1) antibodies in non-symptomatic pregnant mares and neonatal foals
Provedor de dados: 45
Autores: Carvalho,R.; Passos,L.M.F.; Gouvea,A.M.G.; Resende,M.; Martins,A.S.; Franco,G.C.
An ELISA system was applied to study the prevalence of EHV-1 in vaccinated and non-vaccinated Brazilian horses. Serum samples were taken from non-symptomatic pregnant mares, before and after the parturition, and from their neonatal foals. The antigen was prepared from a purified virus fraction (HVE-1 AB1 strain). The ELISA absorbance of 0.3516 was determined as the threshold of negative values. This cut-off level was defined from a selected interval, which determined a sensibility of 100% and a specificity of 94.7%, from a serum collection obtained from pregnant mares and fetuses. In this study, a strong correlation was found between the ELISA and serum neutralization for specific EHV-1 antibody detection.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse; Herpesvirus; ELISA.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000300003

Avaliação da nested PCR em comparação aos testes sorológicos IDGA e ELISA para o diagnóstico da anemia infecciosa equina
Provedor de dados: 45
Autores: Santos,E.M.; Motta,P.M.C.; Heinemann,M.B.; Leite,R.C.; Reis,J.K.P.
Comparou-se a técnica nested PCR (nPCR) com os testes sorológicos IDGA e ELISA para o diagnóstico da anemia infecciosa equina. Amostras do DNA provenientes das células mononucleares do sangue periférico foram submetidas à amplificação do gene gag pela nPCR, que apresentou valores de sensibilidade e especificidade relativas de 90% e 52,9%, respectivamente, em relação à IDGA, e valores de 85,7% e 49%, respectivamente, em relação ao ELISA. Considerando-se os fatores referentes às limitações de cada técnica, pode ser sugerido o uso da nPCR como teste de diagnóstico complementar para AIE em amostras brasileiras.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnóstico; AIE; NPCR; IDGA; ELISA.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000200004

Imunodiagnóstico da cisticercose em suíno experimentalmente infectado com ovos de Taenia solium, utilizando antígeno de escólex de Cysticercus cellulosae
Provedor de dados: 45
Autores: Soares,K.A.; Silva,M.R.M.; Poleti,M.D.; Maia,A.A.M.
Colheu-se sangue de sete suínos infectados com ovos de Taenia solium, semanalmente, durante 140 dias, para realizar ELISA no soro, utilizando antígeno de escólex (Es-Tso) de C. cellulosae. Em todos os animais, após o 21º dia pós-infecção, houve incremento significativo de anticorpos IgG, que assim se mantiveram até o final do experimento. A sensibilidade do ELISA variou entre 87,5 e 100%. À necropsia, foram identificados 238 cisticercos. Seis suínos apresentaram pelo menos um cisto no coração, língua ou masseter. Não se observou correlação entre concentração de anticorpos e número de cisticercos identificados.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suíno; Cisticercose; Cysticercus cellulosae; Antígeno; ELISA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000100003

Levantamento sorológico de Mycobacterium avium subesp. paratuberculosis em bovinos leiteiros no estado do Espírito Santo
Provedor de dados: 45
Autores: Costa,J.C.M; Pieri,F.A; Souza,C.F; Espeschit,I.F; Felippe,A.G; Santos,G.M; Tobia,F.L; Silva Junior,A; Moreira,M.A.S.
The occurrence of antibodies to Mycobacterium avium subsp. paratuberculosis (MAP) was verified in dairy cattle from Espírito Santo state. A total of 1,450 serum samples were analyzed for antibodies anti-MAP, using ELISA. Dairy cattle, males and females, from four regions of Espírito Santo state were used. One hundred sixty-five (11.4%) samples were positive for anti-MAP, 33 (2.3%) were considered suspicious, and 1,252 (86.3%) were negative. In all regions, seropositive animals were found, indicating that the agent is spread by the State, posing a threat to the local dairy farming and neighboring states, as well as public health, since MAP can be involved with Crohn's disease in humans. This result presents the first serologic anti-MAP survey in dairy...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bovino; ELISA; Anticorpos; Ocorrência.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600028

Production of specific polyclonal antibodies anti-human factor IX
Provedor de dados: 52
Autores: Chaves,Daniel Gonçalves; Rodrigues,Cibele Velloso; Ferreira,Wanderley Almeida; Siqueira,Pollyana Fantini Miranda; Duarte,Clara Guerra; Carneiro-Proietti,Anna Bárbara de Freitas; Santoro,Marcelo Matos.
In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Factor IX; Hemophilia B; HPLC; Purification; Antibodies; ELISA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010

Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil
Provedor de dados: 52
Autores: Minozzo,João Carlos; Moura,Juliana de; Almeida,Sérgio Monteiro; Thomaz-Soccol,Vanete.
Neurocysticercosis (NCC), the cerebral presence of Taenia solium metacestode (Cysticercus cellulosae), is responsible for neurological disorders worldwide. In order to validate an immunodiagnosis for public-health patients in the State of Parana-Brazil, crude antigen of Taenia crassicepsmetacestode (Cysticercus longicollis) was used as an alternative heterologous antigen to be used in ELISA and in electroimmunotransfer blotting (EITB) for active and inactive NCC diagnosis. Indirect ELISA was able to discriminate between active and inactive samples and presented high specificity and sensitivity. Any immunodominant band was able to distinguish the NCC stages, although the EITB showed 100% specificity. The immunological results proved to be an important...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurocysticercosis; Immunodiagnosis; ELISA; EITB; Cysticercus longicollis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600007

Immune response after rabies oral immunization in mice
Provedor de dados: 52
Autores: Silva,Camila Sloboda Pacheco da; Krüger,Ernesto Renato; Agottani,Jorge Victor Bacila; Oliveira,Rubens Chaguri de; Pereira,Sandro; Penha,Tânia Regina; Ollhoff,Rüdiger Daniel.
The objective of this study was to formulate an anti-rabies oral vaccine from the cell culture vaccine PV TECPAR to elicit the production of antibodies against the rabies in mice. A vaccine was developed using 10(7.5) DL50/0.03 ml viral antigens homogenised in lanovaseline to facilitate oral administration. Mice were vaccinated two times for seroconversion. Sera of the vaccinated mice showed a higher level of antibody production than the control group. These results could be used to direct the development of an anti-rabies oral vaccine.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Oral vaccine; Mice; ELISA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600006

Production of leptospiral LipL32 antigen in Pichia pastoris and its use in an enzyme-linked immunosorbent assay
Provedor de dados: 52
Autores: Monte,Leonardo Garcia; Leal,Fernanda Munhoz Dos Anjos; Hartwig,Daiane Drawanz; Vasconcellos,Sílvio Arruda; Brihuega,Bibiana; Dellagostin,Odir Antonio; Hartleben,Cláudia Pinho.
The production of recombinant LipL32 protein using Escherichia coli has been used extensively for the development of vaccines and diagnostic tests for leptospirosis. However, E. coli has demonstrated limitations, including low yield and lack of post-translational modifications. In this study, rLipL32 was produced in eukaryotic expression system (Pichia pastoris) and evaluated the antigen by enzyme-linked immunosorbent assay (ELISA). The yield obtained from the culture supernatant reached 270 mg/L and ELISA showed an accuracy of 95.34%. In summary, the production of rLipL32 using P. pastoris did not impair the antigenic characteristics of this antigen and ensured its use for detecting the leptospiral antibodies in swine sera.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pichia pastoris; LipL32; ELISA; Leptospirosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300007

Níveis de IgG séricos em bezerros experimentalmente infectados pelo Haemonchus placei
Provedor de dados: 62
Autores: Nishi,Sandra Mayumi; Richtzenhain,Leonardo José; Gennari,Solange Maria.
Dois experimentos foram realizados para se conhecer a dinâmica dos anticorpos séricos (IgG) em bezerros após infecção com Haemonchus placei. No Experimento 1 estudou-se os níveis de IgG ao longo da primeira e segunda infecção. No Experimento 2 a influência de diferentes níveis protéicos da dieta e a resposta à infecção, medida através dos níveis de IgG séricos, foi observada em bezerros imunizados e não imunizados, através de infecções sucessivas e experimentalmente desafiados com L3 de H. placei. Observou-se que os bezerros infectados desenvolveram resistência a reinfecções, com acentuado decréscimo nos valores de ovos por grama de fezes (OPG) na segunda infecção. Entretanto, nesse mesmo período, os níveis de IgG séricos mantiveram-se elevados,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haemonchus; Bovinos; ELISA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000200010

Comparação das técnicas de ELISA indireto e de soroneutralização na detecção de anticorpos contra o BHV-1 em amostras de soro bubalino (Bubalus bubalis)
Provedor de dados: 62
Autores: CORTEZ,Adriana; HEINEMANN,Marcos Bryan; ALFIERI,Amauri Alcindo; MÉDICI,Kerly Cristina; ALFIERI,Alice Fernandes; OLIVEIRA,Daniela Bergamin; MEYER,Adriana Dresden; SOARES,Rodrigo Martins; SAKAMOTO,Sidnei Mioshi; AMARAL,Renato; BARUSELLI,Pietro Sampaio; FUJII,Takako; RICHTZENHAIN,Leonardo José.
O desempenho de um ELISA comercial (Bovine Rhinotracheitis virus antibody test Kit, Herdcheck®, IDEXX Laboratories Inc., USA) para a identificação de anticorpos anti-herpesvírus bovino tipo 1 (BHV-1), em soro bovino, foi avaliado em 133 amostras de soro de búfalos (Bubalus bubalis), sem histórico de vacinação para IBR, da Região do Vale do Ribeira, Estado de São Paulo. Tomando-se a técnica de soroneutralização como referência, o ELISA indireto apresentou sensibilidade e especificidade relativa de 97,14 e 46,03%, respectivamente. A concordância estimada pelo índice kappa, entre os dois métodos, foi de 0,44 IC (0,29-0,59), utilizando um intervalo de confiança de 95%.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Rinotraquite infecciosa bovina; ELISA; Búfalos; Bubalus bubalis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962001000300010

Serological survey for bovine herpesvirus 1 in cattle from different regions in the state of Bahia, Brazil
Provedor de dados: 62
Autores: CERQUEIRA,Robson Bahia; CARMINATI,Renato; SILVA,Josiane Martins; SOARES,Gubio Campos; MEYER,Roberto; SARDI,Silvia.
The presence of antibodies against BHV-1 was evaluated in young bovines from fifteen different counties distributed along North, South and Central regions of the state of Bahia. Bovines with age ranged from 1 to 4 years old, non-vaccinated against BHV-1 and without diagnostic of BHV-1 infection were chosen for blood collection. A total of 558 serum samples were collected equivalent to 10% of the population in each farm. The sera were submitted to ELISA (HerdCheck-IDDEX) and Microserum neutralization (MSN) tests. The MSN was performed in monolayers of MDBK cells in microplates using Los Angeles BHV-1 strain. The results of serum samples by ELISA showed that 56% (314/558) were positive and 44% (244/558) were negative. The sera assayed by MSN showed that 48%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine herpesvirus 1 (BHV-1); ELISA; Serumneutralization; Diagnosis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000600014

Detecção de anticorpos contra o vírus da diarréia viral bovina no soro sangüíneo, no leite individual e no leite de conjunto em tanque de expansão de rebanhos não vacinados
Provedor de dados: 62
Autores: Dias,Fabio Carvalho; Samara,Samir Issa.
Pela pesquisa de anticorpos contra o vírus da diarréia viral bovina (BVD), utilizando o teste de ELISA indireto, foi estudada a correlação existente entre a proporção de vacas lactantes e a presença de anticorpos no leite de conjunto do tanque de expansão. Para isso foram analisadas amostras de soro sangüíneo e de leite individual de 376 vacas lactantes não vacinadas, provenientes de 10 propriedades localizadas nas regiões Sul do Estado de Minas Gerais e Nordeste do Estado de São Paulo, assim como uma amostra do leite do tanque de expansão de cada rebanho. Em todas as propriedades foram encontradas vacas reagentes no soro sangüíneo, cuja freqüência variou de 12,28 a 100,00%. Já a análise do leite individual não revelou animais reagentes em duas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarréia Viral Bovina; Anticorpos; Leite; Soro sangüíneo; ELISA.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000300001

Serological survey of antibodies to Toxoplasma gondii in food animals from São Paulo state, Brazil
Provedor de dados: 62
Autores: Meireles,Luciana Regina; Galisteo Jr.,Andrés Jimenez; Andrade Jr.,Heitor Franco de.
Toxoplasmosis is one of the most prevalent parasitic infections of man and livestock, and its transmission has usually been attributed to ingestion of undercooked or raw meat from infected livestock, with the infection rate in those animals being an important risk predictor of human disease, high in Brazil and São Paulo State. Looking for this public health problem, we tested serum samples from cattle, goat, sheep and chicken from the State of São Paulo, Brazil, for IgG antibodies to Toxoplasma gondii by enzyme-linked immunosorbent assay (ELISA). Antibodies to Toxoplasma gondii were found in 31.00% (62/200) of sheep, 17.00% (34/200) of goat and 11.00% (22/200) of cattle, without positive sample in chicken. Despite differences in feeding habits of each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Food animals; Seroprevalence; ELISA.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000400005

Ocorrência da diarréia viral bovina nas regiões sul do Estado de Minas Gerais e nordeste do Estado de São Paulo
Provedor de dados: 62
Autores: Samara,Samir Issa; Dias,Fabio Carvalho; Moreira,Sandra Possebon Gatti.
Amostras de soro sangüíneo de 376 vacas lactantes foram analisadas para pesquisa de anticorpos contra o vírus da diarréia viral bovina por meio do teste de ELISA indireto. O estudo foi realizado em 10 propriedades leiteiras localizadas nas regiões sul do Estado de Minas Gerais e nordeste do Estado de São Paulo, entre os meses de março e junho de 2000. Em todas as propriedades foram encontrados animais reagentes e a ocorrência nas duas regiões foi proporcionalmente similar, sendo que na região sul do Estado de Minas Gerais a ocorrência foi de 57,56% e na região nordeste do Estado de São Paulo foi de 56,49%. Foram também analisadas as características das propriedades e dos rebanhos, sendo que as maiores ocorrências foram encontradas nos rebanhos mais simples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarréia Viral Bovina; Soro sangüíneo; ELISA; Anticorpos.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000600007

The investigation of parvovirus B19 infection in patients with haematological disorders by using PCR and ELISA techniques
Provedor de dados: 55
Autores: Us,Tercan; Ozune,Leyla; Kasifoglu,Nilgun; Akgun,Yurdanur.
Parvovirus B19 has a marked tropism for erythroid progenitor cells. This may lead to chronic anemia in predisposed individuals. The purpose of the study was to investigate the frequency of parvovirus B19 infections in patients with diagnosis of haematological disorders. In order to determine the diagnostic use of different markers of parvovirus B19 infection, serum specimens obtained from 79 patients with haematological disorders were tested for specific antibodies and viral DNA through the use of ELISA and PCR techniques. Evidence of parvovirus B19 infection was found in 23/79 (29.1%) patients by demonstrating viral DNA and/or specific IgM antibody. B19 infection was established in 3 of 11 patients with chronic myeloid leukemia, in 3 of 11 acute myeloid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; ELISA; Haematological disorder.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300006

Comparison of dot-ELISA and standard ELISA for detection of Neisseria meningitidis outer membrane complex-specific antibodies
Provedor de dados: 55
Autores: Belo,Elza FT; Farhat,Calil K; Gaspari,Elizabeth N De.
Dot-ELISA using the outer membrane complex antigens of Neisseria meningitidis as a target was standardized for rapid detection of meningococcal-specific antibodies in human serum. We investigated the level of meningococcal-specific IgG, IgA, and IgM in serum using dot-ELISA with outer membrane antigens prepared from Neisseria meningitidis serotype B:4.19:P1.15,3,7,9 (a strain isolated from a Brazilian epidemic). The dot-ELISA is based on the same principles as the standard ELISA and is useful for detection of anti-N. meningitidis B antibodies in serum of patients with meningococcal infections. For the assay, outer membrane complexes (OMCs) were absorbed by nitrocellulose membrane and blocked with a 5% skim milk solution. Serum samples were drawn upon...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neisseria meningitidis; Dot-ELISA; ELISA; Antibodies; Outer membrane complex.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100008

Evaluation of reactive Epstein-Barr virus (EBV) in Iranian patient with different subtypes of multiple sclerosis (MS)
Provedor de dados: 55
Autores: Ramroodi,Nourollah; Niazi,Abbas Ali; Sanadgol,Nima; Ganjali,Zohre; Sarabandi,Vida.
OBJECTIVES: Epstein-Barr virus has been recently associated with the onset of multiple sclerosis, yet understanding how it elicits autoimmunity remains elusive. We investigated the relation between Epstein-Barr virus reactivation and disease development in different subtypes of multiple sclerosis. METHODS: In the present research, we have determined the Epstein-Barr virus-DNA load by quantitative real-time polymerase chain reaction and Epstein-Barr virus antibody levels by EIA technique in both multiple sclerosis patients (n = 78) and healthy controls (n = 123). RESULTS: Our results demonstrated increased titer of both anti-Epstein-Barr virus-IgG and IgM antibodies in patients (91.02% vs 82.11% in controls, p &lt; 0.001 and 14.1% vs 4.06% in controls, p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EBV; MS; Reactivation; QPCR; ELISA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200007

Signal to cut-off (S/CO) ratio and detection of HCV genotype 1 by real-time PCR one-step method: is there any direct relationship?
Provedor de dados: 55
Autores: Albertoni,Guilherme; Arnoni,CP; Araújo,PRB; Carvalho,FO; Barreto,JA.
BACKGROUND: Polymerase chain reaction (PCR) methods play an essential role in providing data related to diagnosis, monitoring and treatment of hepatitis C virus (HCV) infection. EIA results are reported as ''reactive'' or ''non reactive'' and EIA S/CO ratio may also be reported as ''high'' or ''low.'' This study aimed to evaluate the performance of a real-time RT-PCR and assess whether there is relationship between S/CO and PCR results. STUDY DESIGN AND METHODS: Sera from blood donors were analyzed by Enzyme-Linked Immunosorbent Assay (ELISA) and RT-PCR assay to detect HCV infection. RESULTS: The RT-PCR assay to genotypes 1a/b showed an acceptable linear response in serial dilutions. The samples were divided into two groups based on their serological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; HCV genotype 1; Real-time PCR; ELISA; RNA extraction.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200006

A study of iga antibody response to different mycobacterium tuberculosis antigens in the diagnosis and monitoring of pulmonary tuberculosis
Provedor de dados: 55
Autores: Bezerra,Janaina Miranda; Beck,Sandra Trevisan; Kanunfre,Kelly Aparecida; Leite,Olavo Munhoz; Ferreira,Antônio Walter.
We evaluated the performance of the ELISA technique in the detection of IgA antibodies against different Mycobacterium tuberculosis antigenic preparations in serum samples from 49 patients with pulmonary tuberculosis collected before and after the start of specific treatment. The controls consisted of serum samples from healthy patients without any prior contact with the bacteria and serum samples from patients with other pneumopathies. Glycolipid antigen gave the best diagnostic performance, with a sensitivity of 88% and specificities varying from 88 to 100% in the control groups. These antigens constitute a powerful tool for the diagnosis and monitoring of patients with pulmonary tuberculosis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; IgA; Serological diagnosis; ELISA; Glycolipid antigen.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100012

The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA
Provedor de dados: 56
Autores: Barbosa,G.H.T.S.; Santana,E.M.; Almeida,A.M.P.; Araujo,A.M.; Fatibello-Filho,O.; Carvalho Jr.,L.B.
F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 µg of F1 antigen/disc, 3% w/v skim milk in PBS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde makes this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Filter paper; Polyvinyl alcohol; Glutaraldehyde; ELISA; Dot-ELISA; Plague.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700013

Serodiagnosis of Helicobacter pylori infection by Cobas Core ELISA in adults from Minas Gerais, Brazil
Provedor de dados: 56
Autores: Rocha,G.A.; Oliveira,A.M.R.; Queiroz,D.M.M.; Mendes,E.N.; Moura,S.B.; Oliveira,C.A.; Ferrari,T.C.A.
We evaluated the accuracy of a 2nd generation ELISA to detect Helicobacter pylori infection in adults from a developing country in view of variations in sensitivity and specificity reported for different populations. We studied 97 non-consecutive patients who underwent endoscopy for evaluation of dispeptic symptoms. The presence of H. pylori was determined in antral biopsy specimens by culture, by the preformed urease test and in carbolfuchsin-stained smears. Patients were considered to be H. pylori positive if at least two of the three tests presented a positive result or if the culture was positive, and negative if the three tests were negative. Sixty-five adults (31 with peptic ulcer) were H. pylori positive and 32 adults were H. pylori negative....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Serodiagnosis; ELISA.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000005

Development and validation of a genotype 3 recombinant protein-based immunoassay for hepatitis E virus serology in swine
Provedor de dados: 56
Autores: van der Poel,W.H.M.; Pavio,N.; van der Goot,J.; van Es,M.; Martin,M.; Engel,B.
Hepatitis E virus (HEV) is classified within the family Hepeviridae, genus Hepevirus. HEV genotype 3 (Gt3) infections are endemic in pigs in Western Europe and in North and South America and cause zoonotic infections in humans. Several serological assays to detect HEV antibodies in pigs have been developed, at first mainly based on HEV genotype 1 (Gt1) antigens. To develop a sensitive HEV Gt3 ELISA, a recombinant baculovirus expression product of HEV Gt3 open reading frame-2 was produced and coated onto polystyrene ELISA plates. After incubation of porcine sera, bound HEV antibodies were detected with anti-porcine anti-IgG and anti-IgM conjugates. For primary estimation of sensitivity and specificity of the assay, sets of sera were used from pigs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis E virus; Zoonosis; Swine; ELISA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400334

Tissue hyaluronan expression, as reflected in the sputum of lung cancer patients, is an indicator of malignancy
Provedor de dados: 56
Autores: Rangel,M.P.; de Sá,V.K.; Martins,V.; Martins,J.R.M.; Parra,E.R.; Mendes,A.; Andrade,P.C.; Reis,R.M.; Longatto-Filho,A.; Oliveira,C.Z.; Takagaki,T.; Carraro,D.M.; Nader,H.B.; Capelozzi,V.L.
Hyaluronan (HA) shows promise for detecting cancerous change in pleural effusion and urine. However, there is uncertainty about the localization of HA in tumor tissue and its relationship with different histological types and other components of the extracellular matrix, such as angiogenesis. We evaluated the association between HA and degree of malignancy through expression in lung tumor tissue and sputum. Tumoral tissue had significantly increased HA compared to normal tissue. Strong HA staining intensity associated with cancer cells was significant in squamous cell carcinoma compared to adenocarcinoma and large cell carcinoma. A significant direct association was found between tumors with a high percentage of HA and MVD (microvessel density) in tumoral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyaluronic acid; Sputum; Lung cancer; Immunohistochemistry; ELISA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600557

Cost-effective analysis of different algorithms for the diagnosis of hepatitis C virus infection
Provedor de dados: 56
Autores: Barreto,A.M.E.C.; Takei,K.; E.C.,Sabino; Bellesa,M.A.O.; Salles,N.A.; Barreto,C.C.; Nishiya,A.S.; Chamone,D.F.
We compared the cost-benefit of two algorithms, recently proposed by the Centers for Disease Control and Prevention, USA, with the conventional one, the most appropriate for the diagnosis of hepatitis C virus (HCV) infection in the Brazilian population. Serum samples were obtained from 517 ELISA-positive or -inconclusive blood donors who had returned to Fundação Pró-Sangue/Hemocentro de São Paulo to confirm previous results. Algorithm A was based on signal-to-cut-off (s/co) ratio of ELISA anti-HCV samples that show s/co ratio ³95% concordance with immunoblot (IB) positivity. For algorithm B, reflex nucleic acid amplification testing by PCR was required for ELISA-positive or -inconclusive samples and IB for PCR-negative samples. For algorithm C, all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C virus; Immunoblot; ELISA; Polymerase chain reaction; Algorithm; Diagnosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200009

Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA
Provedor de dados: 56
Autores: Lima Barros,A.E.; Almeida,A.M.P.; Carvalho Jr.,L.B.; Azevedo,W.M.
We developed an efficient method to prepare a hybrid inorganic-organic composite based on polyvinyl alcohol (PVA) and polysiloxane using the sol-gel disc technique. Antigen obtained from Yersinia pestis was covalently immobilized onto these discs with glutaraldehyde and used as solid phase in ELISA for antibody detection in serum of rabbits experimentally immunized with plague. Using 1.25 µg antigen per disc, a peroxidase conjugate dilution of 1:4,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. These values are similar to those used for PVA-glutaraldehyde discs, plasticized filter paper discs and the polyaniline-Dacron composite discs. This procedure is comparable to that which utilizes the adsorption of the antigen...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Polysiloxane; Polyvinyl alcohol; Solid phase; Immunodetection; Yersinia pestis; ELISA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400008

Serum regucalcin is a useful indicator of liver injury severity in patients with hepatitis B virus-related liver diseases
Provedor de dados: 56
Autores: Wei,Xinhuan; Yu,Haibin; Zhao,Peng; Xie,Li; Li,Li; Zhang,Jing.
Regucalcin is a soluble protein that is principally expressed in hepatocytes. Studies of regucalcin have mainly been conducted in animals due to a lack of commercially available kits. We aimed to develop an enzyme-linked immunosorbent assay (ELISA) to quantify serum regucalcin in patients with hepatitis B virus (HBV)-related disease. High-titer monoclonal antibodies and a polyclonal antibody to regucalcin were produced, a double-antibody sandwich ELISA method was established, and serum regucalcin was determined in 47 chronic hepatitis B (CHB) patients, 91 HBV-related acute-on-chronic liver failure (HBV-ACLF) patients, and 33 healthy controls. The ELISA demonstrated an appropriate linear range, and high levels of reproducibility, sensitivity, specificity,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Regucalcin; Liver injury; Chronic hepatitis B infection; ELISA; Liver failure.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000605

Comparison of the quantification of caffeine in human plasma by gas chromatography and ELISA
Provedor de dados: 56
Autores: Carregaro,A.B.; Woods,W.E.; Tobin,T.; Queiroz-Neto,A.
In the present study we evaluated the precision of the ELISA method to quantify caffeine in human plasma and compared the results with those obtained by gas chromatography. A total of 58 samples were analyzed by gas chromatography using a nitrogen-phosphorus detector and routine techniques. For the ELISA test, the samples were diluted to obtain a concentration corresponding to 50% of the absorbance of the standard curve. To determine whether the proximity between the I50 of the standard curve and that of the sample would bring about a more precise result, the samples were divided into three blocks according to the criterion of difference, in modulus, of the I50 of the standard curve and of the I50 of the sample. The samples were classified into three...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gas chromatography; ELISA; Caffeine; Plasma; Quantification.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600019

Recombinant 60-kDa heat shock protein from Paracoccidioides brasiliensis: is it a good antigen for serological diagnosis of paracoccidioidomycosis?
Provedor de dados: 56
Autores: Peron,G.; Fernandes,F.F.; Landgraf,T.N.; Martinez,R.; Panunto-Castelo,A.
Paracoccidioides brasiliensis and P. lutzii are fungi that cause paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in South America. For serological diagnosis, although 43-kDa glycoprotein (gp43) is regarded as highly specific for PCM, the occurrence of false negative reactions in sera from patients infected with P. lutzii suggests that preparation with only one antigen is not recommended. Heat shock proteins are feasible alternatives as a second antigen because they are often highly immunogenic. In this study, we evaluated the usefulness of recombinant 60-kDa heat shock protein from P. brasiliensis (rPbHsp60) for the serological diagnosis of PCM. Using western blotting assay, we observed that 77.3% of the sera from PCM patients were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paracoccidioidomycosis; Heat shock protein; Serodiagnosis; ELISA; Western blotting.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400705

Leishmania infantum heat shock protein 83 for the serodiagnosis of tegumentary leishmaniasis
Provedor de dados: 56
Autores: Celeste,B.J.; Angel,S.O.; Castro,L.G.M.; Gidlund,M.; Goto,H.
The serologic assay is an important tool in the diagnosis of leishmaniasis. One of the most commonly used tests is enzyme-linked immunosorbent assay (ELISA). Since total Leishmania promastigotes are used as antigen in the routine assay, false-positive reactions are frequent due to cross-reaction with sera from other diseases, mainly Chagas' disease. Therefore, an antigen that determines less cross-reactivity has been pursued for the serodiagnosis of leishmaniasis. In the present study we analyzed the use of recombinant Leishmania infantum heat shock protein (Hsp) 83 in ELISA for the serodiagnosis of cutaneous (N = 12) and mucocutaneous leishmaniasis (N = 14) and we observed the presence of anti-L. infantum Hsp 83 antibodies in all samples as well as...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leishmania infantum; Sp 83; ELISA; Serodiagnosis; Tegumentary leishmaniasis; Chagas' disease.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100001

Improvement of an in-house ELISA for bovine paratuberculosis serology in Brazil
Provedor de dados: 58
Autores: Marassi,Carla Dray; Fonseca,Leila de Souza; Ristow,Paula; Ferreira,Rachel; Lilenbaum,Walter; Oelemann,Walter M.R.
Paratuberculosis (Johne's disease) is a chronic enteritis in cattle caused by infection with Mycobacterium avium subsp. paratuberculosis (Map). In Brazil, few reports describe the isolation of the organism. The gold standard test for Map is its isolation from tissues or feces. Moreover, bacterial growth is slow and test results are available only after three to five months of incubation Furthermore, shedding of bacilli at levels detectable by fecal culture is irregular and does not occur during the early stages of infection, which compromises the sensitivity of this methodology. The most common immunological tests to identify Map infection are complement fixation test (CFT), agar gel immunodiffusion (AGID) and enzyme-linked immunoassay (ELISA). In Brazil,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paratuberculosis; Cattle; ELISA; Serology; Diagnosis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200004

Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil
Provedor de dados: 58
Autores: Albano,Ana Paula Neuschrank; Klafke,Gabriel Baracy; Brandolt,Tchana Martinez; Da Hora,Vanusa Pousada; Nogueira,Carlos Eduardo Wayne; Xavier,Melissa Orzechowski; Meireles,Mário Carlos Araújo.
Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. brasiliensis in the state. Therefore, this study aimed to evaluate the infection of P. brasiliensis in horses from the mesoregion of Southwest Riograndense, using these animals as sentinels. Serological techniques, such as double immunodiffusion in agar gel (AGID) and indirect ELISA, were performed to detect the anti-gp43 P. brasiliensis antibody in horses from five different farms in the region of Bagé, RS, Brazil. Serology was performed in 200 Pure Blood English horses up to two years of age that were born and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ELISA; Gp-43; Protein G-peroxidase; Paracoccidioidomycosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200513

Molecular characterisation of Aspergillus flavus isolates from peanut fields in India using AFLP
Provedor de dados: 58
Autores: Singh,Diwakar; Radhakrishnan,T.; Kumar,Vinod; Bagwan,N.B.; Basu,M.S.; Dobaria,J.R.; Mishra,Gyan P.; Chanda,S.V.
Aflatoxin contamination of peanut, due to infection by Aspergillus flavus, is a major problem of rain-fed agriculture in India. In the present study, molecular characterisation of 187 Aspergillus flavus isolates, which were sampled from the peanut fields of Gujarat state in India, was performed using AFLP markers. On a pooled cluster analysis, the markers could successfully discriminate among the ‘A’, ‘B’ and ‘G’ group A. flavus isolates. PCoA analysis also showed equivalent results to the cluster analysis. Most of the isolates from one district could be clustered together, which indicated genetic similarity among the isolates. Further, a lot of genetic variability was observed within a district and within a group. The results of AMOVA test revealed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aflatoxin; AMOVA; ELISA; Genetic diversity; Groundnut; PCA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300673

Characterization of Mycoplasma penetrans and Mycoplasma fermentans immunodominant proteins
Provedor de dados: 58
Autores: Bruder,Juliana; Godry,Renata Cristina; Takei,Kioko; Cunha,Regina Ayr Florio da.
Mycoplasmas are a heterogeneous group of the smallest organisms capable of self replication and are known to cause many detrimental diseases in both animals and humans. These wall-less prokaryotes are enveloped by a lipoprotein membrane and their small genomes are sufficient to synthesize molecules required for growth and self-replication. Among sixteen species isolated from humans, Mycoplasma pneumoniae, an agent of primary atypical pneumonia, and the urogenital tract species Mycoplasma hominis,Ureaplasma urealyticum and Ureaplasma parvum have been confirmed to be pathogenic. Mycoplasma penetrans and Mycoplasma fermentans, which are species associated with HIV, have been investigated mainly in research laboratories. In this study we have characterized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma penetrans; Mycoplasma fermentans; LAMP-proteins; ELISA; Western blot.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200007

Comparison of serological methods for the diagnostic of Caprine arthritis-encephalitis (CAE) in Rio de Janeiro, Brazil
Provedor de dados: 58
Autores: Cortez-Moreira,Madelayne; Oelemann,Walter M.R.; Lilenbaum,Walter.
Serum samples of 562 goats were tested for Caprine arthritis-encephalitis using agar gel immunodiffusion test (AGID) and enzyme linked immunosorbent assay (ELISA). Seventy-nine samples (14.1%) were reactive by both testes but 141 (25.1%) were reactive only by ELISA. ELISA, when compared to AGID, presented 100% sensitivity and 70.8% specificity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caprine arthritis-encephalitis; CAE; Goats; ELISA; AGID.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100010

Expression of enterovirus 71 capsid protein VP1 in Escherichia coli and its clinical application
Provedor de dados: 58
Autores: Shi,Mei; Zhou,Yaping; Cao,Limin; Ding,Cuijun; Ji,Yun; Jiang,Qinbo; Liu,Xiping; Li,Xiang; Hou,Xueling; Peng,Hongjun; Shi,Weifeng.
The VPl gene of enterovirus 71 (EV71) was synthesized, construct a recombinant plasmid pET15b/VP1 and expressed in E. coli BL21. The recombinant VP1 protein could specifically react with EV71-infected patient sera without the cross-reaction with serum antibodies of coxsackievirus A16 (CA16), A4, A5, B3 and B5 as well as echovirus 6. In acute and convalescent phases, IgM and IgG antibodies of 182 serum samples were detected by ELISA with recombinant VP1 protein as a coated antigen. The results showed that the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of IgM antibodies in serum samples for the diagnosis of EV71 infection were 90.1, 98.4, 98.8 and 88.7%, respectively; similarly, those of IgG antibodies in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterovirus 71; Gene cloning; Recombinant VPl protein; ELISA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400027

Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane (Saccharum spp.) Using differente polyclonal antibodies
Provedor de dados: 58
Autores: Silva-Froufe,Lúcia Gracinda da; Boddey,Robert Michael; Reis,Veronica Massena.
The species Gluconacetobacterdiazotrophicus, Herbaspirillum seropedicae and H. rubrisubalbicans are endophytic N2-fixing [diazotrophic] bacteria which colonise not only roots, but also the aerial tissue of sugar cane. However, the technique most commonly used to quantify the populations of these microbes in plants is by culturing serial dilutions of macerates of plant tissues in N free semi-solid media which are only semi-selective for the species/genera [the Most Probable Number (MPN) Technique] and each culture must be further subjected to several tests to identify the isolates at the species level. The use of species-specific polyclonal antibodies with the indirect ELISA (enzyme-linked immunosorbent assay) can be an alternative which is rapid and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diazotrophic bacteria; ELISA; Immunoquantification.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400018

Analysis of isotype-specific antibody responses to bovine herpesviruses 1.1 and 1.2a allows to estimate the stage of infection
Provedor de dados: 58
Autores: Spilki,Fernando Rosado; Franco,Ana Cláudia; Roehe,Paulo Michel.
Specific IgM, IgA, IgG1, IgG2, as well as neutralizing antibody responses were evaluated in sera of calves experimentally infected with two isolates of bovine herpesvirus type 1 (BoHV1) of distinct subtypes (subtype 1, BoHV1.1; subtype 2a, BoHV-1.2a). No significant differences were observed in the antibody responses induced by each BoHV-1 subtype. The antibody responses following primary acute infection were characterized by an increase in specific IgM and IgA levels between days 2 and 14 post inoculation (pi). IgG1 was detected from days 11 to 30 pi. IgG2 was detected on the sample taken on day 30 pi. Reactivation of infection following dexamethasone administration induced a significant rise in IgA levels, whereas IgG1 and IgG2 levels, which were at high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine herpesvirus type 1; Infectious bovine rhinotracheitis; Immunoglobulin subclasses; ELISA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200021

An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
Provedor de dados: 58
Autores: Marassi,Carla Dray; Silva,Marley; Oelemann,Walter Martin Roland; Fonseca,Leila de Souza; Ristow,Paula; Lilenbaum,Walter.
ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with comparable results (κ &gt; 0.8) to conventional ELISAs using M. phlei as a preadsorption antigen.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacteria; ELISA; M. fortuitum; M. phlei.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300019

MONOCLONAL ANTIBODIES TO IDENTIFY TOMATO MOSAIC TOBAMOVIRUS (TOMV)
Provedor de dados: 58
Autores: Duarte,Keila M.R.; Gomes,Luiz Humberto; Gesztesi,Jean-Luc; Lopes,José Daniel; Tavares,Flávio C.A.
Monoclonal antibodies were obtained against Tomato mosaic tobamovirus (ToMV) isolated in Brazil. One antibody (8G7G2) isotyped as IgG2b (kappa light chain) showed strong specificity and very low cross reaction with the Tobacco mosaic virus (TMV). It can be used in identification of tomato mosaic virus (ToMV).
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tomato mosaic tobamovirus; ELISA; Monoclonal antibodies.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300016

Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans
Provedor de dados: 58
Autores: Nakanishi,Fernanda Akemi; Avila-Campos,Mario Julio; Kamiji,Nádia Hizuru; Itano,Eiko Nakagawa.
Actinobacillus actinomycetemcomitans produces a protease to human immunoglobulin G that is an important evasion mechanism. In this study, the proteolytic activity of A. actinomycetemcomitans strain ATCC 43718 on human immunoglobulin G associated with culture supernatant concentrations, the growth period and the period of incubation with immunoglobulin G were evaluated by an enzyme linked immunosorbent assay. The protease fraction was detected by Sephadex G 150 chromatography. The results showed that A. actinomycetemcomitans produced a protease to human immunoglobulin G in the culture supernatant, and the highest activity was achieved witen the concentration was 27.5 mug protein/mL, after culturing for 72 hours and incubating with IgG for 24 hours. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunoglobulin G; Actinobacillus actinomycetemcomitans; ELISA; Protease.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100008

Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat
Provedor de dados: 58
Autores: Schneid,Andréa dos Santos; Rodrigues,Kelly Lameiro; Chemello,Davi; Tondo,Eduardo Cesar; Ayub,Marco Antônio Zacchia; Aleixo,José Antonio Guimarães.
In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonellaenterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Monoclonal antibody; Policlonal antibody; ELISA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027

Development of an indirect ELISA based on recombinant capsid protein to detect antibodies to bovine leukemia virus
Provedor de dados: 58
Autores: Andreolla,Ana Paula; Erpen,Luana Marina Scheer; Frandoloso,Rafael; Kreutz,Luiz Carlos.
Abstract Serological testing and culling infected animals are key management practices aiming eradication of bovine leukemia virus infection. Here, we report the development of an indirect ELISA based on BLV recombinant capsid protein (BLVp24r) to detect anti-BLV antibodies in cattle serum. The BLVp24r was expressed in Escherichia coli and purified by affinity chromatography, and then used to set up the ELISA parameters. The Polysorp ® plate coated with 50 ng of antigen/well and bovine serum diluted 1:100 gave the best results during standardization. Using sera from infected and non-infected cattle we set up the cutoff point at 0.320 (OD450 nm) with a sensitivity of 98.5% and specificity of 100.0%. Then, we tested 1.187 serum samples from dairy (736...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine leukemia virus; Retrovirus; Diagnosis; ELISA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500068

Up-regulation of human immune system function by Donkey’s Milk
Provedor de dados: 60
Autores: Taghiloo,Saeid; Allahmoradi,Esmaeil; Sadeghian-Kiadehi,Seyedeh Forough; Omrani-Nava,Versa; Nazar,Eisa; Ebrahimzadeh,Mohammad Ali.
Donkey’s milk represents a good alternative to human milk because of its chemical characteristics similar to those of human’s. In present study, the pro- and anti-inflammatory effects of donkey’s milk were evaluated on peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from 12 young and 12 aged normal subjects. PBMCs were cultured with or without the optimal and non-cytotoxic dose of pasteurized donkey’s milk, and polymyxin B was used to inhibit the possible endotoxin contamination. Following 18 hours incubation, culture supernatants were harvested to measure the secreted Tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), Interleukin-8 (IL-8) and Interleukin-10 (IL-10) by ELISA. Donkey’s milk significantly increased TNF-α (p= 0.01), IL-8...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Donkey’s milk; ELISA; Immune system; Aged subject; Cytokine.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100607

Ensaio imunoenzimático indireto (ELISA) para detecção de anticorpos anti-Rhodococcus equi em potros
Provedor de dados: 65
Autores: Martins,Carla Braga; Bonesso,Maria Antonieta; Lima,Marcelo Mandrá; Ferraz,Luciana Colbachini; Lacerda Neto,José Corrêa de; Machado,Rosângela Zacarias.
A infecção ocasionada pelo Rhodococcus equi é responsável por elevadas taxas de mortalidade e grandes perdas econômicas na equideocultura. Diante da importância dessa enfermidade, este trabalho objetivou a padronização de um ensaio imunoenzimático indireto (ELISA teste) para a detecção de anticorpos anti-Rhodococcus equi em potros (n=16) antes e após a ingestão de colostro de mães vacinadas. Nesse ensaio, foram utilizados dois tipos de antígenos de Rhodococcus equi, o APTX (proteína semi-purificada VapA, extraído pelo detergente Triton X e precipitado em acetona), e o comercial¹. Os resultados da titulação em bloco determinou a concentração ótima dos antígenos em 2,0µg/ml e a diluição única do soro 1:200 para soros de referências negativo, positivo e soros...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potros; ELISA; Rhodococcus equi.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000300020

An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses
Provedor de dados: 65
Autores: Baldani,Cristiane Divan; Machado,Rosangela Zacarias; Botteon,Paulo de Tarso Landgraf; Takakura,Felipe Santoro; Massard,Carlos Luiz.
A crude antigenic preparation of Babesia equi was used to develop and establish the suitability of an enzyme-linked immunosorbent assay (ELISA) for the detection of parasite carriers. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkboard titrations. The specificity and sensitivity of the ELISA were 100 %. A total of 90 serum samples were taken from horses from the Northeast region of São Paulo State and examined for diagnosis of equine B. equi infection by ELISA. Approximately 75% (n=67) of all the horses tested were found serologically positive for B. equi. These results suggest that the ELISA described may prove to be an appropriate serological test for epidemiological studies on B. equi infections in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Babesia equi; Equine piroplasmosis; ELISA.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000500031

SEROLOGICAL SCREENING FOR INFECTIOUS CATTLE DISEASES: II. ASSOCIATION BETWEEN PREVALENCE AND LEVEL OF ELISA RESPONSE
Provedor de dados: 65
Autores: Rojas,José Alfonso Barajas-; Riemann,Hans; Franti,Charles.
A herd of cattle (Holstein-Zebu crosses) was screened every two months by ELISA during a period of two years for IgG antibodies against 19 infectious disease agents. Two hundred and ninety five sera were collected from 157 young animals (0-4 months of age), 1037 sera from 292 developing animals (4-36 months of age) and 1468 sera from 259 producing animals (&gt; 36 months of age). The results indicate that the difference in ELISA between positive and negative tests is associated with the overall prevalence of positive tests. When the prevalence of positive tests is low the difference between positive and negative tests is greater than when the prevalence is intermediate or high. This means that ELISA, presumably other serological tests for IgG antibodies,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serological screening; Cattie diseases; ELISA.
Ano: 1993
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000200014

Monitoramento da presença de Mycoplasma hyopneumoniae em granjas de suínos durante a implementação de programas de erradicação
Provedor de dados: 65
Autores: Tamiozzo,Pablo Jesús; Pelliza,Bibiana Rosa; Carranza,Alicia Isabel; Ambrogi,Arnaldo.
O objetivo deste estudo foi monitorar a presença de M. hyopneumoniae em granjas suínas durante a implementação de programas de erradicação utilizando diferentes técnicas de diagnóstico focalizando no PCR. Trabalhou-se com uma empresa que possuía três granjas, uma parto-terminação (390 matrizes), uma múltiplo-sítio (4100 matrizes) e uma nova granja que povoava suas novas instalações. Nas duas primeiras, foi desenvolvido um programa de despovoamento parcial para erradicar a pneumonia enzoótica suína, a última foi povoada pelos suínos dos anteriores após a erradicação. Nos três rebanhos, os suínos foram monitorados por: sorologia (ELISA), PCR, lesões pulmonares macro e microscópicas e a presença de tosse não produtiva. A ausência de tosse, a baixa porcentagem...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma hyopneumoniae; Pneumonia enzoótica suína (PES); NPCR; ELISA; Lesões pulmonares.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000400025

Elisa indireto na detecção de Salmonella spp. em lingüiça suína
Provedor de dados: 65
Autores: Loguercio,Andrea Pinto; Aleixo,José Antonio Guimarães; Vargas,Agueda Castagna de; Costa,Mateus Matiuzzi da.
Um teste ELISA, baseado em um anticorpo monoclonal (MAb) específico para uma proteína de membrana externa de Salmonella enterica serovar Enteritidis foi comparado com o método de cultivo tradicional na detecção de Salmonella spp. em 110 amostras de lingüiça suína frescal. A prevalência do patógeno nas amostras foi de 11,82% de acordo com o cultivo tradicional. O teste ELISA revelou sensibilidade, especificidade, valores preditivos positivos e negativos de 100%, 98%, 87% e 100%, respectivamente. Tais achados indicam que, comparado ao sistema tradicional, o teste imunológico foi bastante eficaz na determinação de Salmonella spp. em amostras de lingüiças naturalmente contaminadas.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; ELISA; Lingüiça suína.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000600022

Leishmaniose visceral em um canino de Cruz Alta, Rio Grande do Sul, Brasil
Provedor de dados: 65
Autores: Krauspenhar,Cristina; Beck,Cristiane; Sperotto,Vitor; Silva,Aline Alves da; Bastos,Rodrigo; Rodrigues,Lauro.
Descreve-se um caso de leishmaniose visceral em um canino, macho, Akita, cinco anos, que foi atendido no Hospital Veterinário da Universidade de Cruz Alta, RS, após residir três anos no Estado de Alagoas. Clinicamente observou-se emagrecimento progressivo, lesões crostosas na ponta das orelhas, focinho e região periorbital, úlcera na região distal do membro torácico direito, opacidade da córnea, vômitos e eventuais episódios de diarréia. O exame sorológico para leishmaniose pelo método de ELISA resultou positivo. Na avaliação da medula óssea por imprints (corados por Panóptico), foi possível observar formas amastigotas no citoplasma de macrófagos. A histopatologia consistia de reação linfohistioplasmocitária acentuada, com estruturas basofílicas sugestivas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniose visceral; Leishmania sp.; Amastigotas; ELISA; Imunoistoquímica.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000300052

MARKOV CHAIN MODELING OF ENDEMIC CATTLE DISEASES IN THE TROPICS OF MEXICO
Provedor de dados: 65
Autores: Rojas,José Alfonso Barajas; Riemann,Hans; Franti,Charles.
Endemic diseases measured by prevalence of IgG antibodies by ELISA all showed remarkably stable overall prevalences over a study period of 3 years. This however does not mean that incidence is low or absent. Observed transition from the state of being positive for a disease to the state of being negative and vice versa suggested fairly high incidence rates (and rates of waning antibodies) for all 19 disease agents tested for. In spite of this dynamic situation Markov Chain simulations indicate that a steady prevalence is reached in a fairly short time. Markov Chain simulations may also be useful in elucidating seasonal changes in prevalence.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prevalence; Markov Chain; México; ELISA.
Ano: 1993
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000300014

Frequência de anticorpos e fatores de risco para a infecção pelo vírus da diarreia viral bovina em fêmeas bovinas leiteiras não vacinadas na região Amazônica Maranhense, Brasil
Provedor de dados: 65
Autores: Chaves,Nancyleni Pinto; Bezerra,Danilo Cutrim; Sousa,Vanessa Evangelista de; Santos,Hamilton Pereira; Pereira,Hélder de Moraes.
O presente estudo teve como objetivo determinar a frequência e os fatores de risco para a infecção pelo vírus da diarreia viral bovina (BVDV) em fêmeas bovinas leiteiras na região amazônica maranhense. Amostras de soro de animais não vacinados contra o BVDV e provenientes de 40 propriedades foram submetidas à técnica de ELISA indireto. Em cada propriedade avaliada, aplicou-se ainda um questionário epidemiológico para investigar os fatores de risco que poderiam estar associados à infecção pelo vírus. As amostras foram coletadas de animais que apresentavam ou não sinais clínicos sugestivos da infecção pelo BVDV. Das 400 amostras de soro analisadas, 61,5% (n=246) foram reagentes com a detecção de bovinos sorologicamente positivos em 95% (n=38) das...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BVDV; Anticorpos; ELISA; Vacas leiteiras; Fatores de risco.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000600035

Estudo comparativo entre as provas de ELISA e imunofluorescência indireta (IFI) para detectar anticorpos contra Babesia bovis
Provedor de dados: 65
Autores: Martins,João Ricardo; Corrêa,Bartolomeu Lima; Ceresér,Victor Hermes.
Comparou-se o desempenho de um kit de ELISA para detectar anticorpos contra Babesia bovis, em hemoparasita bovino, com o teste de Imunofluorescência Indireta (IFI) usualmente empregado em rotina sorológica. Sobre 97 amostras de soros oriundas de uma região livre de carrapatos e hematozoários, o teste de ELISA demonstrou uma especificidade de 98.9% contra 97.9% do teste de IFI. Ambos os testes apresentaram uma sensibilidade de 100% quando utilizados sobre 22 amostras de soro de bovinos experimentalmente infectados com B. bovis. Os resultados obtidos sobre 1560 amostras colhidas à campo, mostraram uma concordância de 90,1% (1406/1560) entre amostras positivas e negativas, enquanto que 4,2% (66) foram positivas para IFI e negativas para ELISA e 5,6% (88)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sorologia; Babesia bovis; ELISA; IFI.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781996000100021

Resposta sorológica de coelhos imunizados com antígenos de Pythium insidiosum associados a diferentes adjuvantes
Provedor de dados: 65
Autores: Leal,Alexandre Trindade; Santurio,Janio Morais; Leal,Adriana Bardemaker Monteiro; Pinto,Alexandre Machado; Griebeler,Josiane; Flores,Eduardo Furtado; Ferreiro,Laerte; Catto,João Batista.
O Pythium insidiosum é um fungo zoospórico que se desenvolve em locais alagadiços e que pode infectar humanos e animais, principalmente eqüinos. A infecção natural nesta espécie resulta em pitiose clínica, uma doença granulomatosa de difícil tratamento. Uma das opções terapêuticas é a imunoterapia com antígenos obtidos de culturas do agente. Com o objetivo de avaliar o efeito de adjuvantes na resposta sorológica a antígenos do P. insidiosum, 24 coelhos divididos em 4 grupos foram imunizados com antígeno macerado de micélio (AMM) associado a três adjuvantes. Grupo I: hidróxido de alumínio; grupo II: adjuvante de Freund; grupo III: óleo mineral e grupo IV: água destilada -controle. Os tratamentos foram avaliados a diferentes intervalos, quanto à capacidade...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pythium insidiosum; Imunoterapia; Adjuvantes; Coelhos; ELISA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000600017

SEROLOGICAL SCREENING FOR INFECTOUS CATTLE DISEASES: III. CHOICE OF SENTINEL ANIMALS
Provedor de dados: 65
Autores: Rojas,José Alfonso Barajas-; Riemann,Hans; Franti,Charles.
A herd of Cattle (Holstein-Zebu crosses) was screened bimonthly for two years by ELISA for IgG antibodies to infectious disease agents. The herd was composed of 3 age groups: Young animals ( &lt; 4 months of age), developing animals (4-36 months of age) and producing animals ( &amp;gt; 36 months of age). Ali young animals received colostrum within two hours after birth. The overall consistency of ELISA results, i. e agreement between two consecutive tests was about the same for the three age groups and was lowest for infections of intermediate prevalence (40-50%). The interpretation of changes in seroprevalence is most straight forward for young animals. Young animals positive at their first test reflect the immune status of their mothers while young...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ELISA; Seroconversion; Young; Sentinels; Infectious diseases.
Ano: 1993
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000200015

Padronização do teste ELISA baseado em antígeno capsular purificado dos sorotipos 3, 5 e 7 de Actinobacillus pleuropneumoniae
Provedor de dados: 65
Autores: Dutra,Valéria; Piffer,Itamar; Vargas,Agueda Castagna de; Guidoni,Antônio; Klein,Cátia.
Foram padronizados testes de ELISA (Enzyme-linked immunosorbent assay) baseados em antígeno capsular purificado de Actinobacillus pleuropneumoniae sorotipos 3, 5 e 7, prevalentes no Brasil. Para a padronização foram utilizadas amostras de soro provenientes de leitões inoculados com os três sorotipos do agente em estudo, dos quais se colheram amostras de sangue semanais, durante 15 semanas para estudo da dinâmica da síntese de anticorpos. O controle negativo dos testes constituiu-se de um mistura de 130 soros de animais livres de Actinobacillus pleuropneumoniae (App). Os antígenos também foram testados com amostras de soro de animais infectados com outros agentes causadores de doenças respiratórias e vacinados contra rinite atrófica. Os antígenos produzidos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actinobacillus pleuropneumoniae; ELISA; Antígeno capsular.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000200014

Seroprevalence of Pythium insidiosum infection in equine in Rio Grande do Sul, Brazil
Provedor de dados: 65
Autores: Weiblen,Carla; Machado,Gustavo; Jesus,Francielli Pantella Kunz de; Santurio,Janio Morais; Zanette,Régis Adriel; Pereira,Daniela Sabel Brayer; Diehl,Gustavo Nogueira; Santos,Lucila Carboneiro dos; Corbellini,Luis Gustavo; Botton,Sônia de Avila.
ABSTRACT: An epidemiological survey was carried out by performing an Enzyme Linked Immuno Sorbent Assay (ELISA) test to determine the seroprevalence of Pythium insidiosum infection in equine in Rio Grande do Sul State (RS), Brazil. The serological study covered seven geographical regions of RS, classified according to the Instituto Brasileiro de Geografia e Estatística (IBGE). The samples were obtained from official veterinary service (Serviço Veterinário Oficial, SVO) linked to the Secretaria da Agricultura, Pecuária e Agronegócio of RS (SEAPA-RS) to proceed the investigation of equine infectious anemia in 2014. Samples were collected during the months of September and October of 2013, covering the seven geographical regions of RS, and totalized 1,002...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P. insidiosum; Antibody; Occurrence; Horses; ELISA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100126

ELISA FOR BOVINE SCHISTOSOMIASIS VACCINE EVALUATION: A PRELIMINARY REPORT
Provedor de dados: 65
Autores: Aradaib,Imadeldin Elamin; Abbas,Babiker; Osburn,Bennie; Bushara,Hamid Omer; Taylor,Martin.
Six calves were immunized with schistosomula of Schistosoma bovis irradiated at 3 or 20 Kilorad (Krad) and three calves were kept as controls. Twenty four weeks post immunization, three calves (one from the 20 Krad and two from the 3 Krad group) were challenged with normal cercaria of S. bovis. The immune response was monitored by agar gel immunodiffusion (AGID) and the Enzyme Linked Immunosorbent Assay (ELISA) using adult worm antigen. Using AGID, precipitin lines were observed only with sera from challenged animals. Using ELISA, the immune response of the vaccinated calves was first detected by 2-3 weeks, peaking by 6-8 weeks post vaccination. The immune response of the three challenged calves was elevated by 2 weeks post challenge, peaking at 8-10 weeks...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ELISA; Schistosoma bovis; Vaccine.
Ano: 1994
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000300020

Anticorpos contra o vírus da Doença Infecciosa Bursal e detecção do genoma viral em criações de frango de corte e galinhas de quintal no polo avícola da Bahia
Provedor de dados: 65
Autores: Silva,Priscila Sousa da; Sales,Tatiane Santana; Luz,Izabella Ramos da; Maia,Paulo César Costa; Fernandes,Lia Muniz Barretto; Mendes,Caroline de Oliveira.
Este estudo teve como objetivo determinar a frequência de anticorpos e detectar o genoma viral do vírus da Doença Infecciosa Bursal em criações de frangos de corte e em criações de subsistência localizadas em duas regiões do polo avícola da Bahia. Foram coletadas 758 amostras de soro de frangos de corte e 320 amostras de galinhas de quintal para avaliação da frequência de anticorpos utilizando ELISA indireto. Para a detecção e caracterização do vírus foram coletados 6 pools de bursas de Fabrícius em frangos de corte e 3 pools em criações de subsistência, analisados posteriormente com PCR/RFLP. Os resultados revelaram que não há proteção uniforme na criação comercial nas duas regiões estudadas, sugerindo falha na vacinação e desafio com vírus no ambiente....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Birnavírus; ELISA; Sorologia; Biologia molecular; Aves.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600015

Contaminação ambiental e perfil toxigênico de Bacillus cereus isolados em serviços de alimentação
Provedor de dados: 65
Autores: Soares,Celina Mara; Valadares,Geórgio Freesz; Azeredo,Raquel Monteiro Cordeiro de; Kuaye,Arnaldo Yoshiteru.
A avaliação da contaminação ambiental por Bacillus cereus foi realizada em 90 amostras de ar ambiente e em 96 amostras de superfícies de bancadas e de equipamentos, de dois restaurantes institucionais. O microrganismo foi detectado em 84,4% e 44,8% das amostras de ar ambiente e de superfícies, respectivamente. O potencial enterotoxigênico dos isolados foi investigado através da reação da polimerase em cadeia (PCR) para os genes hblA, hblD e hblC (que codificam a hemolisina BL) e para os genes nheA, nheB e nheC (que codificam a enterotoxina não hemolítica - NHE). De um total de 70 isolados investigados, 14,3% foram positivos para os três genes da HBL e 12,8% foram positivos para os três genes da NHE. A produção de NHE também foi verificada através do...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Segurança alimentar; Hemolisina; Enterotoxina diarréica; PCR; ELISA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000200033

Humoral immune response in cattle experimentally infested with larvae of Dermatobia Hominis
Provedor de dados: 65
Autores: Barbosa,Celso Guimarães; Sanavria,Argemiro; Freire,Ronald Bastos.
Six bovines were infested with 60 first instar larvae of Dermatobia hominis. The animals were bleed weekly, and their antibodies levels to D. hominis L1, L2 and L3 instars measured during the time, following the infestation course. The antisera were submitted to a titration against optimal dilutions of antigen coated wells of microplates, previously sensitized with L1, L2 and L3 preparations, respectively. The ELISA assay was used to test single dilutions of antisera, which results were comparatively analyzed with a control of not infested animals. Antibodies against L1 were detected between the first and 21st day post-infestation (DPI) and, from the 42nd DPI on. Anti-L2 antibodies, could be detected on the 21st DPI and from the 35th DPI until...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatobia hominis; Myiasis; Humoral immune response; ELISA.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000300013

Importância da resistência osmótica na estabilidade do antígeno celular de Mycoplasma mycoides subesp. mycoides tipo LC em ensaio imunoenzimático (ELISA)
Provedor de dados: 65
Autores: Lizeu,Jackeline Oliveira Pontes; Danelli,Maria das Graças Miranda.
O trabalho avalia a importância das condições de armazenamento do antígeno celular de Mycoplasma Mycoides subesp. Mycoides Tipo LC no teste de ELISA. Os resultados mostram a importância da preservação da integridade da célula micoplásmica na estabilidade do antígeno empregado no teste.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Resistência osmótica; Mycoplasma Mycoides; ELISA.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000200042

Soroepidemiologia da doença de Newcastle em plantéis de avestruzes dos Estados da Bahia e de São Paulo
Provedor de dados: 65
Autores: Fernandes,Lia Muniz Barretto; Silva,Priscila Sousa da; Ramos,Izabella; Sales,Tatiane Santana; Herval,Elen Fabiane Guimarães; Batinga,Thaís de Brito; Maia,Paulo César Costa; César,André Eduardo Rocha; Doretto Júnior,Luciano; Meyer,Roberto; Freire,Songeli Menezes.
Estudos sorológicos em Avestruzes (Struthio camelus) são ferramentas úteis para analisar os riscos relacionados à Doença de Newcastle nesses plantéis e à avicultura nacional. No presente estudo, amostras de sangue foram obtidas de avestruzes de ambos os sexos, de diferentes faixas etárias e sem apresentação de sintomatologia clínica, criadas nos Estados da Bahia e de São Paulo com o objetivo de avaliar a presença de anticorpos contra o vírus da Doença de Newcastle por meio de ELISA indireto. Foram testadas 339 amostras provenientes do Estado da Bahia e 105 amostras do Estado de São Paulo. Apesar de os proprietários afirmarem que não foi utilizada vacina em seus animais, foi verificada positividade na Bahia de 17,9% e de 4,7% em São Paulo, em avestruzes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avestruz; Doença de Newcastle; ELISA; Diagnóstico.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000100022

Seroprevalence of bovine echinococcusis in Pakistan
Provedor de dados: 65
Autores: Aziz,Fariha; Tasawar,Zahida; Lashari,Mushtaq Hussain.
ABSTRACT: Cystic echinococcosis is a parasitic infection of worldwide distribution, which, despite causing significant loss of health and money, is still a neglected disease. The present study was performed to determine the prevalence of bovine hydatid disease at Research Centre for the Conservation of Sahiwal Cattle, Jahangirabad, Khanewal, Pakistan. Five hundred bovine serum samples were tested for the detection of bovine Echinococcus antibodies (IgG), using the ELISA kits. Antibodies of bovine Echinococcus were detected in 24.2% cattle. Female cattle showed higher disease prevalence (33.2%) compared to (6.02%) in male cattle. There was positive association between sex of cattle and prevalence (P&lt;0.05). Positive correlation between disease prevalence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Echinococcus antibodies; Seroprevalence; ELISA; Age; Sex.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000400501

Ocorrência de anticorpos anti-Neospora caninum em pequenas propriedades leiteiras do Uruguai
Provedor de dados: 65
Autores: Furtado,Agustín; Rosadilla,Diego; Cattáneo,Milton; Bermúdez,Julián; Puentes,Rodrigo.
Neosporose é uma doença de distribuição mundial causada por um protozoário (Apicomplexa, Sarcocystidae), denominado Neospora caninum (N. caninum). Na América Latina, o protozoário foi diagnosticado no Uruguai, Brasil, Argentina, Chile, Paraguai e Peru. No Uruguai a prevalência em rebanhos leiteiros não foi determinada, havendo somente levantamentos sorológicos de algumas regiões do país em propriedades rurais de médio e grande porte. O objetivo deste trabalho foi determinar a presença de animais sorologicamente positivos contra N. caninum em bacias leiteiras de pequenas propriedades com baixos recursos socioeconômicos da zona central do Uruguai (Estados de Durazno e Tacuarembó). Utilizando um Kit de ELISA comercial, foram analisados 734 soros provenientes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neospora caninum; ELISA; Vacas em lactação; Uruguai.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000400020

Densidade populacional de Ralstonia solanacearum em cultivares de batata a campo
Provedor de dados: 65
Autores: Maciel,João Leodato Nunes; Duarte,Valmir; Silveira,José Ricardo Pfeifer.
A ocorrência de populações latentes de Ralstonia solanacearum em plantas de batata (Solanum tuberosum) pode representar fonte de inóculo de potencial desconhecido. Além disso, também é desconhecido se a população latente da bactéria é menor em cultivares resistentes do que em cultivares suscetíveis. Com a finalidade de estudar estes aspectos, um experimento foi conduzido a campo em dois locais no Estado do Rio Grande do Sul, Brasil. O objetivo foi verificar se havia relação entre a densidade populacional e o grau de resistência de cultivares de batata. O experimento foi conduzido em Eldorado do Sul, durante o período de primavera, e em Caxias do Sul, durante o período de outono. Tubérculos das cultivares Achat, Baronesa, Elvira, Macaca, Monte Bonito e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Murcha bacteriana; Solanum tuberosum; ELISA; Imunofluorescência.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000100004