Consulta: subjectFacets:"Inflammation"
Registros recuperados: 154
Data/hora: 08/06/2024 02:43:40

Rapamycin pre-treatment abrogates Tumour Necrosis Factor-&amp;#945; down-regulatory effects on LXR-Î± and PXR mRNA expression via inhibition of c-Jun N-terminal kinase 1 activation in HepG2 cells
Provedor de dados: Electronic Journal of Biotechnology
Autores: Ng,Di-Lin; Tie,Shin-Wei; Ong,Pei-Chin; Lim,Wyi-Sian; Tengku-Muhammad,Tengku-Sifzizul; Choo,Quok-Cheong; Chew,Choy-Hoong.
The Liver X Receptor (LXR) and Pregnane X Receptor (PXR) are members of the nuclear receptor superfamily. Previously, they have been classified as important regulators of lipid homeostasis. However, recent studies have shown that they may be implicated in anti-inflammatory responses as well. This study shows that Tumour Necrosis Factor-Î± (TNF-Î±) treatment reduces both LXR-Î± and PXR mRNA expression. However, pre-treatment with rapamycin, an mTOR inhibitor, followed by TNF-Î± stimulation, significantly induces LXR-Î± and PXR mRNA expression to ~17- and ~2-fold, respectively. This suggests that mTORC1, a multi-molecular complex of which mTOR is a member, may act as a negative regulator that inhibits the induction of LXR-Î± and PXR as anti-inflammatory...
Tipo: Journal article
Palavras-chave: Akt; C-Jun; Homeostasis; Inflammation; MKK7; Transcription factor.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300008

FISH IMMUNE SYSTEM
Provedor de dados: Gayana
Autores: Olabuenaga,Susana E.
The defence against infections is the essential function of the immune system of all Vertebrates. Fish present an efficient and developed immunologic response and in the case of Teleosts, with some similarities to the higher vertebrates. It can be divided into two types: innate or non specific and adquired or specific, both with a humoral and cellular compartments. Their relative importance can change according to the age of fish and is always under the influence of different factors. The most relevant components are mentioned here. There are two factors in fishes which strongly influence the immune response and antibody production, seasonal changes and temperature. The main lymphoid organs are described here: the thymus, the kidney and the spleen, as well...
Tipo: Journal article
Palavras-chave: Antigen; Antibody; Leukocytes; Phagocytosis; Lymphokines; Inflammation; Immunostimulants.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200010

Alternatively spliced MEFV transcript lacking exon 2 and its protein isoform pyrin-2d implies an epigenetic regulation of the gene in inflammatory cell culture models
Provedor de dados: Genetics and Molecular Biology
Autores: Erdem,Gokce Celikyapi; Erdemir,Sule; Abaci,Irem; Aydin,Asli K. Kirectepe; Everest,Elif; Turanli,Eda Tahir.
Abstract The function of gene body DNA methylation in alternative splicing, and its relation to disease pathogenesis is not fully elucidated. The gene for familial Mediterranean fever (MEFV) encodes the pyrin protein and contains a 998 bp CpG island, covering the second exon, which is differentially methylated in FMF patients compared to healthy controls. Our further observation of increased exon 2-spliced MEFV transcript in leukocytes of FMF patients provoked us to test the role of exon methylation in alternative splicing using inflammatory cell culture models. First, in vitro exon methylation triggered an increased level of exon 2 exclusion using a splicing cassette in a promyelocytic leukemia cell line (HL-60). HL-60 cells subjected to methylating and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exon methylation; MEFV; Pyrin; Alternative splicing; Inflammation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400688

Anti-inflammatory Effect of Silymarin on Ovarian Immunohistochemical Localization of TNF- Associated with Systemic Inflammation in Polycystic Ovarian Syndrome
Provedor de dados: International Journal of Morphology
Autores: Kayedpoor,Parvin; Mohamadi,Shima; Karimzadeh-Bardei,Latifeh; Nabiuni,Mohammad.
Tumor necrosis factor alpha (TNF-a) and interleukin (IL)-6, are prominent mediators of inflammation and have been confirmed to be elevated in at least a subgroup of women with polycystic ovary syndrome (PCOS). In this study, the effects of Silymarin (SLM) on the expression TNF-a, IL-6, CRP and symptoms of PCOS were studied. In this research, PCOS was induced by injection of Estradiol Valerate. PCOS rats were divided into control and experimental groups received intraperitoneal injection SLM extract daily. After syndrome induction, ovaries were collected for histological and immunohistochemical evaluations. Serum IL-6 was detected by the ELISA kit. The results indicated the significant reduction in inflammatory markers and significant changes follicular...
Tipo: Journal article
Palavras-chave: Immunohistochemistry; Inflammation; Silybum Marianum extract.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200054

Effects of Fetal Programming in the Inflammatory Response in Wistar Rats: A Systematic Review
Provedor de dados: International Journal of Morphology
Autores: Assis,Thiago de Oliveira; Araújo Filho,José Candido de; Maia,Maria Bernadete de Sousa.
The aim of this study was to review the literature on the effects of fetal programming in the inflammatory response in Wistar rats. A search was performed in the following databases: PubMed, MEDLINE, PUBMED, SCIENCE DIRECT, SCOPUS, LILACS, SpringerLink. The main search terms were malnutrition and inflammation in Portuguese and in English. Original articles were included involving albino rats and review articles were excluded involving humans or animals other than rats. Articles that were related to malnutrition which was not intrauterine and did not involve the concept of fetal programming were also excluded.Those items found in more than one database were counted only once. Sixteen articles were found in PUBMED, 16 in SCOPUS, 4 in MEDLINE, 341 in SCIENCE...
Tipo: Journal article
Palavras-chave: Fetal Programming; Inflammation; Wistar Rats; Systematic review.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000400048

Fractal Analysis of Skeletal Muscle Tissue of Rats Subjected to Stretch Injury
Provedor de dados: International Journal of Morphology
Autores: Ozaki,Guilherme; Camargo,Regina Celi Trindade; Koike,Tatiana Emy; Garcia,Thiago Alves; Castoldi,Robson Chacon; Camargo Filho,Jose Carlos Silva.
The aim of this study was to analyze changes in the skeletal muscle tissue of rats after muscle stretch injury using fractal analysis. Nineteen rats were divided into three groups, Control (n= 5) (C), Lesion two days (n= 7) (L2), and Lesion seven days (n= 7) (L7). Histological slides of the gastrocnemius muscle of the animals were prepared and stained with Hematoxylin and eosin for analysis of the inflammatory process, and picrosirius for the demonstration of collagen. The stained slides were subsequently analyzed using Fractal Dimension (FD). The results showed high FD values of the inflammatory process in the experimental groups L2 and L7 in relation to C (p= 0.000). The analysis of collagen in the picrosirius stained slides showed high FD in the L2...
Tipo: Journal article
Palavras-chave: Fractal analysis; Skeletal muscle injury; Collagen; Inflammation; Pathology.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300017

Histological Changes Implicated in Metastasis
Provedor de dados: International Journal of Morphology
Autores: Díaz-Peña,Roberto; Castro-Santos,Patricia.
The process of malignancy emergence is associated with the acquisition of the capacity to invade other tissues. Several different biological processes have been described as involved in this process. Specifically, epithelial mesenchymal transition (EMT), a mechanism associated with embryogenesis and wound repair but also with mobility acquisition, is one of the concerned processes. In EMT an epithelial cell loses its epithelial characteristics, its junctions with neighbor cells and with the basal lamina and acquires mobility and mesenchymal characteristics. Also, factors of the tumor microenvironment have been described as involved. Tumor presence triggers a response in the surrounding tissue known as reactive stromal. It shows particular characteristics...
Tipo: Journal article
Palavras-chave: EMT; CAF; Metastasis; Cancer; Inflammation.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300031

The Effect of Visfatin on Inflammatory Reaction in Uterus of LPS-Induced Rats
Provedor de dados: International Journal of Morphology
Autores: Yang,Zhi; Xiao,Ke; Wang,Wei; Tang,Juan; Sun,Peng-Peng; Peng,Ke-Mei; Song,Hui.
The present study was to investigate the effects of visfatin on the morphological structure and function of the rat uterus during inflammation. The expression and distribution of visfatin, morphological structure, eosinophils (EOS), myeloperoxidase (MPO) and cytokines in the uterus of the LPS-induced rat were studied using hematoxylin-eosin staining (HE), immunohistochemical methods, western blots and enzyme-linked immunosorbent assay (ELISA). The present study showed that visfatin positive cells dispersed widely in the uterus, and strong positive staining was observed mainly in the cell cytoplasm. Compared with saline group, in visfatin group, more uterine glands were found, EOS increased, and the difference was significant (P&lt;0.05), MPO reduced, and...
Tipo: Journal article
Palavras-chave: Visfatin; LPS-induced rat; Uterus; Inflammation; Cytokine.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100031

ANTITUMOR EFFECT OF SNAKE VENOMS
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: SILVA,R. J. DA; FECCHIO,D.; BARRAVIERA,B.
The search for biological antitumor agents has been pursued for over half a century. Snake venom has been shown to possess a wide spectrum of biological activities. The objectives of the present review are to evaluate the existing controversies on this subject published in a number of papers and to propose probable explanations for the phenomena observed. We reported our results obtained in a study, in which we evaluated the action of the venoms of Crotalus durissus terrificus and Bothrops jararaca on Ehrlich ascites tumor cells. We noticed an important antitumor effect, mainly with Bothrops jararaca venom, as well as an increase in the functional activity of macrophages. We also observed an increase in the number of mononuclear and polymorphonuclear cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cancer; Inflammation; Venom; Snakes; Crotalus durissus terrificus; Bothrops jararaca.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301996000200002

EFFECT OF Crotalus durissus terrificus (LAURENTI, 1768) VENOM ON THE EVOLUTION OF EHRLICH ASCITES TUMOR
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: SILVA,R. J. DA; FECCHIO,D.; BARRAVIERA,B.
The effect of Crotalus durissus terrificus (LAURENTI, 1768) venom on the evolution of Ehrlich ascites tumor cells was evaluated. Thus, 30-day-old male mice of the Swiss strain were inoculated intraperitoneally with 1x10&lt;img SRC="http:/img/fbpe/jvat/v3n2/image1947.gif"&gt; tumor cells. Then, 7 groups of animals were formed: 3 control groups (physiological, venom and tumor) and 4 experimental groups that received different doses of venom. The experimental groups received 5 intraperitoneal venom injections on the 1&lt;img SRC="http:/img/fbpe/jvat/v3n2/image1948.gif"&gt; , 4&lt;img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"&gt; , 7&lt;img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"&gt; , 10&lt;img SRC="http:/img/fbpe/jvat/v3n2/image1949.gif"&gt; and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venom; Inflammation; Tumor growth; Crotalus durissus terrificus.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000200008

ACUTE-PHASE REACTIONS, INCLUDING CYTOKINES, IN PATIENTS BITTEN BY BOTHROPS AND CROTALUS SNAKES IN BRAZIL
Provedor de dados: Journal of Venomous Animals and Toxins
Autores: BARRAVIERA,B.; LOMONTE,B.; TARKOWSKI,A.; HANSON,L. Å.; MEIRA,D.A.
Thirty-one patients bitten by venomous snakes in Botucatu area (State of São Paulo - Brazil), sixteen by Bothrops spp. and fifteen by Crotalus durissus terrificus, were studied. The group comprised twenty-nine males and two females, ranging from fourteen to sixty-three years of age (mean 33 ± 15). Leukocytosis with neutrophilia and lymphopenia, increase of mucoproteins and C-reactive protein, decrease of total serum protein and albumin, were observed on the first day after the accident. In addition, increased serum levels of the cytokines IL-6 and IL-8, but not of IL-1&lt;FONT FACE="Symbol"&gt;b&lt;/FONT&gt; and TNF-&lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;, were observed. The alterations were generally more intense in patients bitten by Crotalus durissus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Acute-phase reactions; Cytokines; C-reactive protein; Inflammation.
Ano: 1995
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301995000100003

Inflammatory response, parasite load and AgNOR expression in ear skin of symptomatic and asymptomatic Leishmania (Leishmania) chagasi infected dogs
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: BLA,Verçosa; ACJ,Borges; FJM,Mendes; MLS,Costa; NB,Pereira; MN,Melo; IL,Mendonça; FAL,Costa; AC,Vasconcelos.
The skin has an important role in the transmission of visceral leishmaniasis (VL) as the infection pathway in dogs. To better characterize the inflammatory response of intact skin in VL, sixty infected dogs (30 symptomatic and 30 asymptomatic) and six non-infected controls were studied. Diagnosis of visceral leishmaniasis was confirmed by RIFI and ELISA; direct visualization of the parasite in bone marrow aspirate; imprints of popliteal lymph nodes, spleen, liver and skin; culture in NNN-phase liquid Schneider's medium; and PCR (performed only in the ear skin). Amastigote forms of the parasite in intact skin were found only in symptomatic dogs. Inflammatory infiltrates were observed in all groups, varying from intense and/or moderate in symptomatic to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Inflammation; Parasites; Leishmania chagasi.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300011

Preliminary molecular characterization of a proinflammatory and nociceptive molecule from the Echinometra lucunter spines extracts
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Sciani,Juliana Mozer; Zychar,Bianca; Gonçalves,Luis Roberto; Giorgi,Renata; Nogueira,Thiago; Pimenta,Daniel Carvalho.
Abstract  Background  Sea urchins are animals commonly found on the Brazilian shoreline, being Echinometra lucunter the most abundant species. Accidents caused by E. lucunter have been reported as one of the most frequent in Brazil, and are characterized by intense pain and inflammation, consequence of spine puncture in the skin. In order to characterize such toxic effects, we isolated one molecule that caused inflammatory and nociceptive effects.  Methods E. lucunter specimens were collected without gender distinction. Spines were removed and molecules were extracted, fractionated by RP-HPLC and assayed for inflammatory and nociceptive activity, in a biological-driven fractionation way, until the obtainment of one active molecule and its subsequent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxins; Sea urchin; Echinometra lucunter; Spines; Inflammation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100318

rACLF, a recombinant snake venom metalloprotease, activates endothelial cells in vitro
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: De Moraes,C. K.; Fritzen,M.; Chudzinski-Tavassi,A. M.; Selistre-de-Araújo,H. S.
Snake venom metalloproteases (SVMPs) comprise a family of snake venom toxins responsible for most of local and systemic effects observed during envenomation by snakes from the Viperidae family. The vascular system and more specifically the endothelium seem to be the preferential targets of these proteins. This work describes the effects of rACLF, a recombinant SVMP from Agkistrodon contortrix laticinctus on human umbilical vein endothelial cells (HUVECs) in vitro. Our results showed that rACLF activates HUVECs by the release of mediators involved in inflammation and hemostasis such as prostacyclin and interleukin-8. We also demonstrated that rACLF increased the expression of ICAM-I and decay accelerating factor (DAF). Moreover, rACLF protects the HUVECs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RACLF; Snake venom metalloprotease; Endothelial cells; Inflammation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100009

Immunotherapeutic potential of Crotoxin: anti-inflammatory and immunosuppressive properties
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Sartim,Marco Aurélio; Menaldo,Danilo Luccas; Sampaio,Suely Vilela.
Abstract For the past 80 years, Crotoxin has become one of the most investigated isolated toxins from snake venoms, partially due to its major role as the main toxic component in the venom of the South American rattlesnake Crotalus durissus terrificus. However, in the past decades, progressive studies have led researchers to shift their focus on Crotoxin, opening novel perspectives and applications as a therapeutic approach. Although this toxin acts on a wide variety of biological events, the modulation of immune responses is considered as one of its most relevant behaviors. Therefore, the present review describes the scientific investigations on the capacity of Crotoxin to modulate anti-inflammatory and immunosuppressive responses, and its application as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus; Crotoxin; Innate immunity; Adaptative immunity; Inflammation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100329

Cytotoxic and inflammatory potential of a phospholipase A2 from Bothrops jararaca snake venom
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Cedro,Rafhaella C. A.; Menaldo,Danilo L.; Costa,Tássia R.; Zoccal,Karina F.; Sartim,Marco A.; Santos-Filho,Norival A.; Faccioli,Lúcia H.; Sampaio,Suely V.
Abstract  Background: Snake venom phospholipases A2 (PLA2s) have been reported to induce myotoxic, neurotoxic, hemolytic, edematogenic, cytotoxic and proinflammatory effects. This work aimed at the isolation and functional characterization of a PLA2 isolated from Bothrops jararaca venom, named BJ-PLA2-I.  Methods and Results: For its purification, three consecutive chromatographic steps were used (Sephacryl S-200, Source 15Q and Mono Q 5/50 GL). BJ-PLA2-I showed acidic characteristics, with pI~4.4 and molecular mass of 14. 2 kDa. Sequencing resulted in 60 amino acid residues that showed high similarity to other Bothrops PLA2s, including 100% identity with BJ-PLA2, an Asp49 PLA2 previously isolated from B. jararaca venom. Being an Asp49 PLA2, BJ-PLA2-I...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops jararaca; Phospholipase A2; Inflammation; Cytotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100324

Immunopathology of rabies infection in mice selected for high or low acute inflammatory reaction
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Achkar,S. M.; Sinhorini,I. L.; Ribeiro,O. G.; Carrieri,M. L.; Ceretta,R. S.; Consales,C. A.
Rabies is a severe and lethal disease that produces a slight inflammatory response during the infection process. We analyzed the immunopathological mechanisms that occur in the central nervous system (CNS) using mice genetically selected for maximal or minimal acute inflammatory reaction (AIRmax or AIRmin). As viral samples, we adopted the antigenic variant 3 (AgV3) of rabies virus from hematophagous bats and a fixed virus strain (PV1 43/3). Titration of specific antibodies was performed using enzyme-linked immunosorbent assay (ELISA). We observed a slight increase in IgG and IgG1 isotypes in infected AIRmax mice. Incubation period, determined by intracerebral inoculation with 100 LD50, was 6-7 days for PV1 43/4 strain and 9-10 days for AgV3. No difference...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Virus replication; Pathology; Inflammation; Immunoglobulin isotypes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100003

Effect of Bothrops alternatus snake venom on macrophage phagocytosis and superoxide production: participation of protein kinase C
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Setubal,SS; Pontes,AS; Furtado,JL; Kayano,AM; Stábeli,RG; Zuliani,JP.
Envenomations caused by different species of Bothrops snakes result in severe local tissue damage, hemorrhage, pain, myonecrosis, and inflammation with a significant leukocyte accumulation at the bite site. However, the activation state of leukocytes is still unclear. According to clinical cases and experimental work, the local effects observed in envenenomation by Bothrops alternatus are mainly the appearance of edema, hemorrhage, and necrosis. In this study we investigated the ability of Bothrops alternatus crude venom to induce macrophage activation. At 6 to 100 ¼g/mL, BaV is not toxic to thioglycollate-elicited macrophages; at 3 and 6 ¼g/mL, it did not interfere in macrophage adhesion or detachment. Moreover, at concentrations of 1.5, 3, and 6 ¼g/mL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops; Macrophages; Phagocytosis; Superoxide; Protein kinase C; Inflammation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400010

Anti-inflammatory activity of the apolar extract from the seaweed Galaxaura marginata (Rhodophyta, Nemaliales)
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Rozas,E.; Freitas,J. C.
The red seaweed Galaxaura marginata (Ellis &amp;amp; Solander) Lamouroux, well known by the antibacterial activity of its polar extract and the cytotoxic activity of its oxygenated desmosterol, showed anti-inflammatory action in its apolar fraction. Topical anti-inflammatory activity was observed in samples collected at São Sebastião channel, northern littoral of São Paulo State, Brazil. The apolar extract and its fractions obtained through Thin-Layer Chromatography (TLC) reduced the topical inflammation produced by croton oil in mouse ear. Such data indicated that the apolar extract from the marine red alga G. marginata displayed anti-inflammatory activity (since 1mg/ear extract reduced 95±0.5% inflammation), which could be the result of the synergic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Seaweed; Galaxaura marginata; Inflammation; Croton oil; Edema; Anti-inflammatory agents.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200011

Intradermal injection of Bothrops cotiara venom in mice in an experimental wound model
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Lopes,JA; Giménez,APL; Zischler,LFCM; Stuelp-Campelo,PM; Moreno,AN; Elifio-Esposito,SL.
Bothropic envenomation induces hemorrhage, coagulant disturbances and necrosis. Regarding therapies against the local damage caused by the venom, there is little information on tissue changes until the complete healing. In the current study, local damage was evaluated by examination of morphological inflammatory alterations, mast cell count, and analysis of collagen deposition. Bleeding was evident four hours after inoculation. After 24 hours, a large area of injury appeared presenting disorganized tissue, significant hemorrhage and acute inflammation. After three days, the damaged area was extensive, with a large amount of inflammatory cells and the presence of scab. In seven days, healing and reepithelization process started. And, 21 days later, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops venom; Wound healing; Inflammation; Local damage.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100009

Anti-inflammatory activity of Blutaparon portulacoides ethanolic extract against the inflammatory reaction induced by Bothrops jararacussu venom and isolated myotoxins BthTX-I and II
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Pereira,IC; Barbosa,AM; Salvador,MJ; Soares,AM; Ribeiro,W; Cogo,JC; Zamuner,SR.
This article reports the anti-inflammatory effect of Blutaparon portulacoides (B. portulacoides), specifically the ethanolic extract of its aerial parts, on the edema formation and leukocyte influx caused by Bothrops jararacussu (B. jararacussu) snake venom and Bothropstoxin-I and II (BthTX-I and II) isolated from this venom as an alternative treatment for Bothrops snakebites. The anti-inflammatory effect of B. portulacoides ethanolic extract was compared with an animal group pretreated with dexamethasone. B. portulacoides ethanolic extract significantly inhibited paw edema induced by B. jararacussu venom and by BthTX-I and II. Also, results demonstrated that the extract caused a reduction of the leukocyte influx induced by BthTX-I. However, the extract...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Myotoxins; Inflammation; Edema; Blutaparon portulacoides.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300013

Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Barbosa,AM; Villaverde,AB; Guimarães-Sousa,L; Soares,AM; Zamuner,SF; Cogo,JC; Zamuner,SR.
The prominent myotoxic effects induced by Bothrops jararacussu crude venom are due, in part, to its polycationic myotoxins, BthTX-I and BthTX-II. Both myotoxins have a phospholipase A2 structure: BthTX-II is an active enzyme Asp-49 PLA2, while BthTX-I is a Lys-49 PLA2 devoid of enzymatic activity. In this study, the effect of low-level laser therapy (LLLT), 685 nm laser at a dose of 4.2 J/cm2 on edema formation, leukocyte influx and myonecrosis caused by BthTX-I and BthTX-II, isolated from Bothrops jararacussu snake venom, was analyzed. BthTX-I and BthTX-II caused a significant edema formation, a prominent leukocyte infiltrate composed predominantly by neutrophils and myonecrosis in envenomed gastrocnemius muscle. LLLT significantly reduced the edema...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Myotoxins; Inflammation; Myonecrosis; Low-level laser therapy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300014

Propolis: a review of its anti-inflammatory and healing actions
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Ramos,A. F. N.; Miranda,J. L.
Tissue healing is an adaptive biological response by which the organism repairs damaged tissue. The initial stage of healing is represented by an acute inflammatory reaction, in which inflammatory cells migrate to damaged tissue and phagocyte debris. At a later stage, fibroblasts and endothelial cells proliferate and generate a scar. The occurrence of inflammatory processes and healing imperfections have been a concern for hundreds of years, especially for individuals with healing difficulties, such as diabetics and carriers of peripheral circulation deficiencies. A wide variety of natural products have been used as anti-inflammatory and healing agents, with propolis being a remarkable option. Propolis has been used in popular medicine for a very long...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Inflammation; Anti-inflammatory action; Healing properties; Apis mellifera.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400002

Effects of tea on survival rates and liver pathology of Trypanosoma brucei brucei infected mice
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: Mbuthia, S.K; Wachira, N.W; Ngure, R.M; Ouma, J; Kagira, J.M.
The current study investigated the effects of different types of Kenyan tea extracts on the pathogenesis of Trypanosoma brucei brucei in a Swiss White mice model. Following infection with trypanosomes, the mice were monitored for survival and liver pathology. Tea significantly (P&lt;0.05) enhanced the survival rate of tea treated mice. Additionally, in tea treated but infected mice, there was reduction in infiltration of inflammatory cells into the periportal and parenchymal regions as well as hepatocyte cell damage compared to the infected untreated animals. Green and white teas were superior in most of the above effects while black tea and oolong teas had the least effects. The tea extracts were more efficacious than dexamethasone in prolonging the life...
Palavras-chave: Inflammation; Liver pathology; Survival rate; Tea; Trypanosomiasis.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3094

Vitamin C and endogenous cortisol in foreign-body inflammatory response in pacus
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Belo,Marco Antonio de Andrade; Moraes,Julieta Rodini Engrácia de; Soares,Vando Edésio; Martins,Maurício Laterça; Brum,Camila Dantas; Moraes,Flávio Ruas de.
The objective of this work was to evaluate the effect of food supplementation with vitamin C on macrophage and multinucleated giant cell (MGC) activities of pacus at two stocking densities. The experiment was carried out in a 2x2x3 split-plot factorial arrangement with: 0 and 500 mg kg-1 vitamin C; 5 and 20 kg m-3 stocking densities; and evaluation times at 3, 6, and 12 days after the subcutaneous implantation of glass coverslips (DPI). The number of macrophages and MGC, as well as cortisol and glucose plasma levels were determined. The number of macrophages and MGC with two to five nuclei was significantly greater in fish supplemented with vitamin C at 5 kg m-3 stocking density at 3 DPI in comparison to nonsupplemented ones. The macrophage and MGC counts...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piaractus mesopotamicus; Ascorbic acid; Glucocorticoid; Inflammation; Langhans cells; Multinucleated giant cells.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000700019

Avian cytokines in health and disease
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Wigley,P; Kaiser,P.
Cytokines are proteins secreted by cells that play an important role in the activation and regulation of other cells and tissues during inflammation and immune responses. Although well described in several mammalian species, the role of cytokines and other related proteins is poorly understood in avian species. Recent advances in avian genetics and immunology have begun to allow the exploration of cytokines in health and disease. Cytokines may be classified in a number of ways, but may be conveniently arranged into four broad groups on the basis of their function. Proinflammatory cytokines such as interleukin-6 and interleukin-1beta play a role in mediating inflammation during disease or injury. Th1 cytokines, including interleukin-12 and interferon-gamma,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Immunity; Infectious disease; Inflammation; Vaccination.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000100001

Acute inflammatory response in Nile tilapia fed probiotic Lactobacillus plantarum in the diet - doi: 10.4025/actascibiolsci.v33i3.8011
Provedor de dados: Acta Scientiarum: Biological Sciences
Autores: Dotta, Geovana; Laboratório AQUOS-Sanidade de Organismos Aquáticos, Depto. de Aqüicultura, CCA, UFSC - Rod. Admar Gozaga 1346 - 88040-900 - Florianópolis, SC - Brasil; Mouriño, José Luis Pedreira; Laboratório de Camarões Marinhos, Depto. de Aqüicultura, CCA, UFSC, Beco dos Coroas, Barra da Lagoa - Florianópolis, SC - Brasil; Jatobá, Adolfo; Laboratório de Camarões Marinhos, Depto. de Aqüicultura, CCA, UFSC, Beco dos Coroas, Barra da Lagoa - Florianópolis, SC - Brasil; Burgos Morán, Ricardo Ernesto; Universidad Politécnica de Catalunya, Campus del Baix Llobregat, Av. del Canal Olímpic, s/n - 08860 - Castelldefels, Catalunya - España; Pilati, Celso; UFSC; Martins, Maurício Laterça; UFSC.
The present study evaluated the acute inflammatory response induced by carrageenin (500 &amp;micro;g) injected in the swim bladder of Nile tilapia, after fed or not probiotic supplemented diet. Fifty four fish were distributed in six treatments and three replicates: Group A: Fish fed unsupplemented diet: 0.5 mL saline-injected fish; fish injected with 500 &amp;micro;g carrageenin diluted in 0.5 mL saline; Non-injected. Group B: Fish fed probiotic supplemented diet: saline-injected fish; carrageenin-injected fish; Non-injected. Fifteen days after feeding the fish were injected with carrageenin or saline. After six hours, inflammatory exudate was collected, as well as the blood for hematocrit, red blood cell (RBC) and white blood cell (WBC) counts,...
Tipo: Composição bioquímica
Palavras-chave: 2.00.00.00-6 Ciencias Biologicas Tilapia; Probiotic; Lactobacillus plantarum; Inflammation; Haematology; Phagocytosis 2.00.00.00-6 Ciencias Biologicas Tilapia; Probiotic; Lactobacillus plantarum; Inflammation; Haematology; Phagocytosis.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8011

Consequences of gestational diabetes to the brain and behavior of the offspring
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: SOUSA,RICARDO A.L. DE; TORRES,YASMIN S.; FIGUEIREDO,CLAUDIA P.; PASSOS,GISELLE F.; CLARKE,JULIA R.
ABSTRACT Gestational diabetes mellitus (GD) is a form of insulin resistance triggered during the second/third trimesters of pregnancy in previously normoglycemic women. It is currently estimated that 10% of all pregnancies in the United States show this condition. For many years, the transient nature of GD has led researchers and physicians to assume that long-term consequences were absent. However, GD diagnosis leads to a six-fold increase in the risk of developing type 2 diabetes (T2D) in women and incidence of obesity and T2D is also higher among their infants. Recent and concerning evidences point to detrimental effects of GD on the behavior and cognition of the offspring, which often persist until adolescence or adulthood. Considering that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin resistance; Hippocampus; Inflammation; Depression; Learning; Memory; Programing.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502279

Candesartan inhibits inflammation through an angiotensin II type 1 receptor independent way in human embryonic kidney epithelial cells
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: YU,YING; JIANG,HAIFENG; NIU,YANGYANG; ZHANG,XIAOQIN; ZHANG,YINGYING; LIU,XI; QI,TAO; YU,CHEN.
Abstract: Besides stimulating vasoconstriction, Angiotensin II is also well known in inducing reactive oxygen species and promoting inflammatory phenotype switch via its type 1 receptor. In clinic, Angiotensin II type 1 (AT1) receptor blocker like candesartan has been widely applied as an antihypertensive medication. We previous have demonstrated that a higher dose of candesartan plays a protective role after kidney injury. However, whether candesartan could exhibit anti-inflammatory effects remains unclear. Here, by stimulating isolated human embryonic kidney epithelial cells with tumor necrosis factor-α (TNF-α), we observed the anti-inflammation capacity of candesartan ex vivo. It was found that pre-treat with candesartan significantly suppressed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II type 1 receptor blockers; Candesartan; Inflammation; Reactive oxygen species.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300701

B-1 cell: the precursor of a novel mononuclear phagocyte with immuno-regulatory properties
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Lopes,José Daniel; Mariano,Mario.
Characterization of the origin, properties, functions and fate of cells is a fundamental task for the understanding of physiological and pathological phenomena. Despite the bulk of knowledge concerning the diverse characteristics of mammalian cells, some of them, such as B-1 cells, are still poorly understood. Here we report the results obtained in our laboratory on these cells in the last 10 years. After showing that B-1 cells could be cultured and amplified in vitro, a series of experiments were performed with these cells. They showed that B1 cells reside mostly in the peritoneal and pleural cavities, migrate to distant inflammatory foci, coalesce to form giant cells and participate in granuloma formation, both in vitro and in vivo. They are also able to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B-1 cells; IL-10; Inflammation; Infection; Neoplasia; BALB/Xid mice.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300013

Antinociceptive and anti-inflammatory effects of a Geissospermum vellosii stem bark fraction
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: LIMA,JOSÉLIA A.; COSTA,THIAGO W.R.; SILVA,LEANDRO L.; MIRANDA,ANA LUÍSA P.; PINTO,ANGELO C.
ABSTRACT Geissospermum vellosii (Pao pereira) is a Brazilian tree whose stem barks are rich in indole alkaloids that present intense anticholinesterase activity. The present study evaluated the effects of a stem bark fraction (PPAC fraction) and ethanolic extract (EE) of Pao pereira in classic murine models of inflammation and pain. The EE and PPAC fraction, both at a dose of 30 mg/kg, significantly reduced mice abdominal constriction induced by acetic acid by 34.8% and 47.5%, respectively. In the formalin test, EE (30 mg/kg) and PPAC fraction (30 and 60 mg/kg) inhibited only the second phase, by 82.8%, 84.9% and 100%, respectively. Compared with indomethacin, similar doses of EE or PPAC fraction were approximately twice as effective in causing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Geissospermum vellosii; Pao pereira; Antinociception; Inflammation; Cholinergic anti-inflammatory pathway.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100237

An overview of chagasic cardiomyopathy: pathogenic importance of oxidative stress
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Zacks,Michele A.; Wen,Jian-Jun; Vyatkina,Galina; Bhatia,Vandanajay; Garg,Nisha.
There is growing evidence to suggest that chagasic myocardia are exposed to sustained oxidative stress-induced injuries that may contribute to disease progression. Pathogen invasion- and replication-mediated cellular injuries and immune-mediated cytotoxic reactions are the common source of reactive oxygen species (ROS) in infectious etiologies. However, our understanding of the source and role of oxidative stress in chagasic cardiomyopathy (CCM) remains incomplete. In this review, we discuss the evidence for increased oxidative stress in chagasic disease, with emphasis on mitochondrial abnormalities, electron transport chain dysfunction and its role in sustaining oxidative stress in myocardium. We discuss the literature reporting the consequences of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chagasic cardiomyopathy; Reactive oxygen species; Inflammation; Mitochondria; Oxidant/antioxidant status; Oxidative damage.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000400009

Antioxidant, analgesic and anti-inflammatory effects of lavender essential oil
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: SILVA,GABRIELA L. DA; LUFT,CAROLINA; LUNARDELLI,ADROALDO; AMARAL,ROBSON H.; MELO,DENIZAR A. DA SILVA; DONADIO,MÁRCIO V.F.; NUNES,FERNANDA B.; AZAMBUJA,MARCOS S. DE; SANTANA,JOÃO C.; MORAES,CRISTINA M.B.; MELLO,RICARDO O.; CASSEL,EDUARDO; PEREIRA,MARCOS AURÉLIO DE ALMEIDA; OLIVEIRA,JARBAS R. DE.
Several studies have investigated the antinociceptive, immunomodulatory and anti-inflammatory properties of compounds found in the lavender essential oil (LEO), however to date, there is still lack of substantial data. The objective of this study was to assess the antioxidant, anti-inflammatory and antinociceptive effects of lavender essential oil. The 1,1-diphenyl-2-picrylhydrazyl radical decolorization assay was used for antioxidant activity evaluation. The anti-inflammatory activity was tested using two models of acute inflammation: carrageenan-induced pleurisy and croton oil-induced ear edema. The antinociceptive activity was tested using the pain model induced by formalin. LEO has antioxidant activity, which is dose-dependent response. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Inflammation; Lavender; Nociception.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301397

Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: KWON,DA HYE; JEONG,JIN WOO; CHOI,EUN OK; LEE,HYE WON; LEE,KI WON; KIM,KI YOUNG; KIM,SUNG GOO; HONG,SU HYUN; KIM,GI-YOUNG; PARK,CHEOL; HWANG,HYE-JIN; SON,CHANG-GUE; CHOI,YUNG HYUN.
ABSTRACT Mori folium, the leaf of Morus alba L. (Moraceae), has been traditionally used for various medicinal purposes from ancient times to the present. In this study, we examined the effects of water extract of Mori folium (WEMF) on the production of inflammatory mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. Our data indicated that WEMF significantly suppressed the secretion of NO and PGE2 in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects were accompanied by a marked reduction in their regulatory gene expression at the transcription level. WEMF attenuated LPS-induced intracellular ROS production in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mori folium; Inflammation; ROS; Macrophage; Zebrafish.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200661

Evaluation in vivo of biocompatibility of differents resin-modified cements for bonding orthodontic bands
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: MESQUITA,JANAINA A.; LACERDA-SANTOS,ROGÉRIO; SAMPAIO,GÊISA A.M.; GODOY,GUSTAVO P.; NONAKA,CASSIANO F.W.; ALVES,POLLIANNA M.
ABSTRACT The focus of this study was to test the hypothesis that there would be no difference between the biocompatibility of resin-modified glass ionomer cements. Sixty male Wistar rats were selected and divided into four groups: Control Group; Crosslink Group; RMO Group and Transbond Group. The materials were inserted into rat subcutaneous tissue. After time intervals of 7, 15 and 30 days morphological analyses were performed. The histological parameters assessed were: inflammatory infiltrate intensity; reaction of multinucleated giant cells; edema; necrosis; granulation reaction; young fibroblasts and collagenization. The results obtained were statistically analyzed by the Kruskal-Wallis and Dunn test (P&lt;0.05). After 7 days, Groups RMO and Transbond...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dental materials; Orthodontic cements; Biocompatibility test; Inflammation; Multinucleated giant cells.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502433

Early fragmentation of polyester urethane sheet neither causes persistent oxidative stress nor alters the outcome of normal tissue healing in rat skin
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: SGROTT,SANDRO M.; NEVES,RODRIGO D.; D’ACAMPORA,ARMANDO J.; BERNARDES,GERALDO J.S.; BELMONTE,LUIZ; MARTINS,THIAGO C.; BOBINSKI,FRANCIANE; CARGNIN-FERREIRA,EDUARDO; HOEPERS,ANDREZA; COMIM,CLARISSA M.; MARTINS,DANIEL F.; PIOVEZAN,ANNA P.
Abstract Silicone breast implant is associated with complications inherent to the surgical procedure. Prosthesis coating with polyurethane, however, commonly reduces the incidence of such complications. In this paper, the authors evaluated the inflammatory histomorphometric profile and oxidative damage associated to the implant of polyester urethane sheets. Forty-eight Wistar rats were divided into Sham or polyester urethane groups (n = 8/group) and underwent a polyester urethane implant in the dorsal skinfold. Tissue samples were collected on days seven, 30, and 90 after surgery and subjected to histomorphometric analysis and biochemical tests. Results were analyzed by one-way ANOVA (p ≤ 0.05). Peri-implant tissue samples exhibited characteristic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyurethane; Inflammation; Cytokines; Oxidative stress; Wistar rats.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502211

Cyclic nitroxides inhibit the toxicity of nitric oxide-derived oxidants: mechanisms and implications
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Augusto,Ohara; Trindade,Daniel F.; Linares,Edlaine; Vaz,Sandra M.
The substantial therapeutic potential of tempol (4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy) and related cyclic nitroxides as antioxidants has stimulated innumerous studies of their reactions with reactive oxygen species. In comparison, reactions of nitroxides with nitric oxide-derived oxidants have been less frequently investigated. Nevertheless, this is relevant because tempol has also been shown to protect animals from injuries associated with inflammatory conditions, which are characterized by the increased production of nitric oxide and its derived oxidants. Here, we review recent studies addressing the mechanisms by which cyclic nitroxides attenuate the toxicity of nitric oxidederived oxidants. As an example, we present data showing that tempol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclic nitroxides; Tempol; Antioxidants; Nitric oxide-derived oxidants; Inflammation; Acetaminopheninduced hepatotoxicity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000100013

Assessment of Anti-inflammatory and Antioxidant Properties of Safranal on CCI4-Induced Oxidative Stress and Inflammation in Rats
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: ALAYUNT,ÖMER N.; AKSOY,LAÇINE; KARAFAKIOĞLU,YASEMIN S.; SEVIMLI,SEVCAN.
Abstract: The present study aimed to determine the antioxidative and anti-inflammatory effects of safranal on damage induced by CCl4. Experimental animals were divided into five groups. The first group was determined as the control group and no treatment was conducted. Second group rats were administered 1 mL/kg-day CCI4 during the experiment. Rats in Groups 3, 4 and 5 were administered 1 mL/kg-day CCI4 and 25 mg/kg, 50 mg/kg; 100 mg/kg safranal, respectively via gavage. Oxidative-antioxidant parameters, liver function enzymes and inflammatory cytokine levels were determined in liver samples obtained from the rats. Data analysis demonstrated that oxidative stress and inflammation markers were significantly higher in CCI4 administered groups (p&lt;0.05)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; CCI4; Inflammation; Safranal.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300704

Association of IL-6 and CRP gene polymorphisms with obesity and metabolic disorders in children and adolescents
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Todendi,Pâmela F.; Klinger,Elisa I.; Ferreira,Michele B.; Reuter,Cézane P.; Burgos,Miria S.; Possuelo,Lia G.; Valim,Andréia R.M.
Activation of adipose tissue inflammation is associated with obesity caused by lipid accumulation in adipocytes. Through this activation, proinflammatory cytokines, such as Interleukin-6 (IL-6) and C-reactive protein (CRP) seem to influence metabolic disorders. The present study evaluated whether polymorphisms in the CRP (rs1205) and IL-6 (rs1800795, rs2069845) genes are associated with the development of metabolic disorders in children and adolescents. A cross-sectional study was performed, consisting of 470 students from the municipality of Santa Cruz do Sul, Brazil, aged 7-17 years. Body mass index (BMI) was classified according to overweight and obesity. Genotyping was performed by real-time Polymerase Chain Reaction(PCR). Anthropometric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Obesity; Polymorphisms; Risk; Schoolchildren.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200915

In vivo biocompatibility of silicon dioxide nanofilm used as antimicrobial agent on acrylic surface
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: LACERDA-SANTOS,ROGÉRIO; LIMA,ANTONIA BÁRBARA L.; PENHA,ELIZANDRA S. DA; SANTOS,ANTONIELSON DOS; CARVALHO,FABÍOLA G.; PITHON,MATHEUS M.; DANTAS,ANTÔNIO FLÁVIO M.
Abstract the focus ofthis study was to testthe hypothesisthatthere would be no difference betweenthe biocompatibility of silicon dioxide nanofilms used as antimicrobial agents. Sixty male Wistar rats were divided into 4 groups (n=15): Group C (Control,Polyethylene), Group AR (Acrylic Resin), Group NP (Acrylic Resin coated with NP-Liquid), Group BG (Acrylic Resin coated with Bacterlon).the animals were sacrificed with 7,15 and 30 days and tissues analyzed as regardsthe events of inflammatory infiltrate, edema, necrosis, granulation tissue, mutinucleated giant cells, fibroblasts and collagen. Kruskal-Wallis and Dunn tests was used (P&lt;0.05). Intense inflammatory infiltrate was shown mainly in Groups BG and AR, with significant difference from Control Group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomaterials; Biocompatibility; Dentistry; Inflammation.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100910

Dextran Sulphate of Sodium-induced colitis in mice: antihyperalgesic effects of ethanolic extract of Citrus reticulata and potential damage to the central nervous system
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: HILEL,ALEXANDRE S.; GYSEMANS,BRUNA; LISBÔA,MARIA E.M.; HEYMANNS,ANA C.; FREIBERGER,VIVIANE; VENTURA,LETÍCIA; MAGNAGO,RACHEL F.; COMIM,CLARISSA M.; HOREWICS,VERÔNICA; MARTINS,DANIEL F.; BOBINSKI,FRANCIANE; PIOVEZAN,ANNA P.
Abstract Citrus species are widely related to antihyperalgesic and anti-inflammatory effects. The aim of this study was to investigate if treatment with ethanolic extract from peels of mature Citrus reticulata Blanco causes antihyperalgesic effects on the referred mechanical hyperalgesia in a model of dextran sulphate of sodium (DSS)-induced colitis in mice, as well as the possible oxidative damage in different regions of the brain induced by its inflammatory reaction. Antihyperalgesia (30 to 300 mg/kg) was investigated by behavioral response (frequency of response to von Frey filament stimulation) in Swiss mice, while damage to central nervous system was investigated through techniques that evaluated oxidative stress using male black C57 BL6 mice (n=8)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus reticulate; Medicinal plants; Inflammation; Oxidative stress; Central nervous system.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000603139

Subcutaneous administration of ketoprofen delays Ehrlich solid tumor growth in mice
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Souza,C.M.; Auler,P.A.; Reis,D.C.; Lavalle,G.E.; Ferreira,E.; Cassali,G.D.
Ketoprofen, a nonsteroidal anti-inflammatory drug (NSAID) has proven to exert anti-inflammatory, anti-proliferative and anti-angiogenic activities in both neoplastic and non-neoplastic conditions. We investigated the effects of this compound on tumor development in Swiss mice previously inoculated with Ehrlich tumor cells. To carry out this study the solid tumor was obtained from cells of the ascites fluid of Ehrlich tumor re-suspended in physiological saline to give 2.5x106cells in 0.05mL. After tumor inoculation, the animals were separated into two groups (n = 10). The animals treated with ketoprofen 0.1µg/100µL/animal were injected intraperitoneally at intervals of 24h for 10 consecutive days. Animals from the control group received saline. At the end...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Cell proliferation; Ketoprofen; Neoplasm.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501376

Metabotropic glutamate receptor 5 may be involved in macrophage plasticity
Provedor de dados: Biological Research
Autores: Shanshiashvili,Lali; Tsitsilashvili,Elene; Dabrundashvili,Nino; Kalandadze,Irine; Mikeladze,David.
Abstract  Background  Macrophages are a functionally heterogeneous cell population and depending on microenvironments they polarize in two main groups: M1 and M2. Glutamic acid and glutamate receptors may participate in the regulation of macrophage plasticity. To investigate the role of glutamatergic systems in macrophages physiology, we performed the transfection of mGluR5 cDNAs into RAW-264.7 cells.  Results  Comparative analysis of modified (RAW-mGluR5 macrophages) and non-modified macrophages (RAW-macrophages) has shown that the RAW-mGluR5 macrophages absorbed more glutamate than control cells and the amount of intracellular glutamate correlated with the expression of excitatory amino acid transporters -2 (EAAT-2). Besides, our results have shown that...
Tipo: Journal article
Palavras-chave: Macrophages; Metabotropic glutamate receptors; Inflammation; HMGB1; IL-10.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100203

Structural biology of chemokine receptors
Provedor de dados: Biological Research
Autores: ROJO,DANIEL; SUETOMI,KATSUTOSHI; NAVARRO,JAVIER.
Chemokine receptors are G protein-coupled receptors that mediate migration and activation of leukocytes as an important part of a protective immune response to injury and infection. In addition, chemokine receptors are used by HIV-1 to infect CD4 positive cells. The structural bases of chemokine receptor recognition and signal transduction are currently being investigated. High-resolution X-ray diffraction and NMR spectroscopy of chemokines indicate that all these peptides exhibit a common folding pattern, in spite of its low degree of primary-sequence homology. Chemokines' functional motifs have been identified by mutagenesis studies, and a possible mechanism for receptor recognition and activation is proposed, but high-resolution structure data of...
Tipo: Journal article
Palavras-chave: Chemokines; G-protein coupled receptors; Inflammation; HIV-1.
Ano: 1999
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400006

In vivo and in vitro anti-inflammatory activity of Cryptostegia grandiflora Roxb. ex R. Br. leaves
Provedor de dados: Biological Research
Autores: Castro,Jenny P; Ocampo,Yanet C; Franco,Luis A.
BACKGROUND: Despite Cryptostegia grandiflora Roxb. ex R. Br. (Apocynaceae) leaves are widely used in folk Caribbean Colombian medicine for their anti-inflammatory effects, there are no studies that support this traditional use. Therefore, this work aimed to evaluate the effect of the total extract and primary fractions obtained from Cryptostegia grandiflora leaves, using in vivo and in vitromodels of inflammation, and further get new insights on the mechanisms involved in this activity. RESULTS: Ethanolic extract of Cryptostegia grandiflora leaves, and its corresponding ether and dichloromethane fractions, significantly reduced inflammation and myeloperoxidase activity (MPO) in ear tissue of mice treated with 12-O-tetradecanoyl-phorbol-13-acetate (TPA)....
Tipo: Journal article
Palavras-chave: Ear edema; Free radicals; Inflammation; MPO; PGE2; RAW 264; 7 macrophages.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100032

Preventive effect of Ligularia fischeri on inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages depending on cooking method
Provedor de dados: Biological Research
Autores: Park,Hee-Sook; Choi,Hye-Young; Kim,Gun-Hee.
BACKGROUND: Ligularia fischeri (common name Gomchwi) is known for its pharmaceutical properties and used in the treatment of jaundice, scarlet-fever, rheumatoidal arthritis, and hepatic diseases; however, little is known about its anti-inflammatory effect. In this study the influence of blanching and pan-frying on the anti-inflammatory activity of Ligularia fischeri (LF) was evaluated. RESULTS: Fresh LF and cooked LF showed no significant effect on the viability of macrophages after 24 h incubation. Fresh LF was found to be the most potent inhibitor of nitric oxide (NO) production at 100 &amp;#956;g/ml, while pan-fried LF showed little inhibitory effect on lipoloysaccharide (LPS) stimulated murine machrophage RAW264.7 cells. In contrast with its effect on...
Tipo: Journal article
Palavras-chave: Chlorogenic acid; Inflammation; Ligularia fischeri; Macrophage.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100063

Translational control of ceruloplasmin gene expression: Beyond the IRE
Provedor de dados: Biological Research
Autores: MAZUMDER,BARSANJIT; SAMPATH,PRABHA; FOX,PAUL L.
Translational control is a common regulatory mechanism for the expression of iron-related proteins. For example, three enzymes involved in erythrocyte development are regulated by three different control mechanisms: globin synthesis is modulated by heme-regulated translational inhibitor; erythroid 5-aminolevulinate synthase translation is inhibited by binding of the iron regulatory protein to the iron response element in the 5'-untranslated region (UTR); and 15-lipoxygenase is regulated by specific proteins binding to the 3'-UTR. Ceruloplasmin (Cp) is a multi-functional, copper protein made primarily by the liver and by activated macrophages. Cp has important roles in iron homeostasis and in inflammation. Its role in iron metabolism was originally proposed...
Tipo: Journal article
Palavras-chave: Translational control; Ceruloplasmin; Iron homeostasis; GAIT element; Inflammation.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100007

Infection imaging with radiopharmaceuticals in the 21st century
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Das,Satya S.; Hall,Anne V.; Wareham,David W.; Britton,Keith E.
Infection continues to be a major cause of morbidity and mortality worldwide. Nuclear medicine has an important role in aiding the diagnosis of particularly deep-seated infections such as abscesses, osteomyelitis, septic arthritis, endocarditis, and infections of prosthetic devices. Established techniques such as radiolabelled leucocytes are sensitive and specific for inflammation but do not distinguish between infective and non-infective inflammation. The challenge for Nuclear medicine in infection imaging in the 21st century is to build on the recent trend towards the development of more infection specific radiopharmaceuticals, such as radiolabelled anti-infectives (e.g. 99mTc- ciprofloxacin). In addition to aiding early diagnosis of infection, through...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopharmaceuticals; Imaging; Infection; Inflammation; Radiolabelled anti-infectives; 99mTc- ciprofloxacin.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500005

Comparative study of the effects of the Ga-As (904 nm, 150mW) laser and the pulsed ultrasound of 1 MHz in inflammation of tibialis muscle of Wistar rats
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Pinto,Marcus Vinícius de Mello; Costa,Daniel Almeida da; Rocha,Lamara Laguardia Valente; Santos,Hélio Ricardo dos; Silva,André Luis dos Santos; Barbosa,Luis Guilherme; Reis,João Batista Alves dos; Bernardo-Filho,Mario.
This paper aims to compare the therapeutic effect of the laser As-Ga of 904nm and pulsed Ultrasound of 1 MHz applied in striated skeletal muscle of inflamed rats. The animals received an intramuscular injection of bupivacaine hydrochloride in tibialis muscle in order to induce the inflammatory process, and after 24 hours, the time was considered 0 for the initiation of therapy, using a laser and ultrasound. Samples collected the muscles of the animals were stained with Hematoxylin-Eosin and histological sections of the groups used for the analysis of the muscle tissue in relation to reducing the inflammatory process, comparing the results of the two therapies used. In this study it is suggested that both treatment with laser as with ultrasound can act as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lasers; Ultrasound; Inflammation; Rats.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700037

The Evolving Role of Erythropoietin in Lung of Irradiated Rats
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Ashry,Omaima Mohamed; Hasan,Hesham Farouk.
ABSTRACT The aim of the present study is to evaluate the possible curative effect of erythropoietin (EPO) on radiation-induced damage to the lung and its renin angiotensin system. EPO (200 U/100g) was i.p. injected to male rats one hour post 6 Gy whole body gamma irradiation. The animals were sacrificed after 14 days post irradiation. Irradiation induced significant drop of haematological values, bone marrow (BM) count, lung oxidative stress markers, glutathione (GSH) and superoxide dismutase(SOD) associated with significant elevation of malondialdehyde (MDA), advanced oxidation protein product (AOPP) and nitric oxide (NO) besides serum inflammatory markers, tumor necrosis factor alpha (TNF-α) and lactate dehydrogenase (LDH). Also serum and lung renin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erythropoietin; Radiation; Inflammation; Oxidative stress.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100312

Bone Marrow Mesenchymal Stem Cells Regulate Coagulation and Inflammation Together in Methotrexate Induced Lung Injury Rat Model
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Haggag,Nawal Zakaria; El-Shinnawy,Nashwa Ahmed; Abd-Elhalem,Sahar Sobhy; Rashed,Laila Ahmed.
Abstract Clinical research has shed the light on the relation between coagulation and inflammation. Coagulation cascade is activated in lung injury resulting in thrombotic and fibrotic lesions. Such a cascade is initiated by inflammation, then the two systems intense each other. New therapies that modulate coagulation and inflammation will be more successful than therapies targeting only one of them. Mesenchymal stem cells showed anti-inflammatory functions in animal models. The role of mesenchymal stem cells in methotrexate induced lung injury model was evaluated, but no studies scoped on the role of stem cells in coagulation associated with inflammation in such models. This study focuses on the therapeutic role of mesenchymal stem cells against the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulation; Inflammation; Lung injury; Mesenchymal Stem Cells; Methotrexate.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100307

Histopathological, immunohistochemical and ultraestructural evaluation of inflammatory response in Arius genus fish under experimental inoculation of BCG
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Sado,Ricardo Yuji; Matushima,Eliana Reiko.
The aim of this study was to evaluate the inflammatory response kinetics after experimental inoculation with BCG in the primitive Arius sp. fish. The BCG was applied through the intramuscular injection in the caudal peduncular region, and the samples were collected for the analyses at days 1, 3, 7, 14, 21, and 33 post-injection. Acute phase inflammatory infiltrate was characterized by the predominant mononuclear cells, intersticial edema, and muscular tissue necrosis. As the inflammatory response evolved, a large number of multinuclear giant cells were formed containing the BCG. These giant cells were positive for the S100 protein at the histochemical analysis, which demonstrate the macrofage activity, confirmed by the ultra-structural analysis showing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Arius; Phylogeny; BCG; Inflammation; Giant cells.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500009

Analgesic and anti-inflammatory properties of essential oil from Ageratum fastigiatum
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Del-Vechio-Vieira,Glauciemar; Sousa,Orlando Vieira de; Miranda,Mariza Abreu; Senna-Valle,Luci; Kaplan,Maria Auxiliadora Coelho.
The chemical composition, analgesic and anti-inflammatory properties of essential oil from Ageratum fastigiatum were investigated. The main compounds found in the essential oil were germacrene D, α-humulene and β-cedrene. The oil, with LD50 of 2.50 g/kg, inhibited the acetic acid-induced writhing at the dose of 200 mg/kg. In the formalin test, the oil inhibited the first phase (200 mg/kg) and the second phase (100 mg/kg and 200 mg/kg). In the hot plate test, after 30 and 60 min of treatment the doses of 100 and 200 mg/kg increased the reaction time. The antiedematogenic effect, reduction on the exudate volume and leukocyte mobilization were observed at the doses of 100 and 200 mg/kg. The results indicated that A. fastigiatum possessed the analgesic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ageratum fastigiatum; Essential oil; Nociception; Inflammation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500008

Antinociceptive and Anti-inflammatory Activities of Marine Sponges Aplysina Caissara, Haliclona sp. and Dragmacidon Reticulatum
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Hort,Mariana Appel; Silva Júnior,Flavio Manoel Rodrigues da; Garcia,Edariane Menestrino; Peraza,Gianni Goulart; Soares,Andrea; Lerner,Clea; Muccillo-Baisch,Ana Luiza.
ABSTRACT Marine sponges are a rich source of bioactive natural products with multiple pharmacological properties. In this study, the anti-inflammatory and antinociceptive effects of extracts obtained from Aplysina caissara, Haliclona sp. and Dragmacidon reticulatum were evaluated by using the writhing test and formalin-induced mouse paw edema model in mice. All extracts were administered via oral pathway in the doses of 60 and 90 mg/kg. In the writhing test the pre-treatment with all sponges resulted in significant inhibition of the acetic acid-induced response, suggesting an antinociceptive effect. The formalin test showed that the extracts from A. caissara, Haliclona sp. and D. reticulatum, in the tested doses, did not affect the first formalin phase,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Nociception; Marine sponges.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100433

Radiopharmaceuticals for imaging chronic lymphocytic inflammation
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Malviya,Gaurav; Vries,Erik F.J. de; Dierckx,Rudi A.; Signore,Alberto.
In the last few decades, a number of radiopharmaceuticals for imaging inflammation have been proposed that differ in their specificity and mechanism of uptake in inflamed foci as compared to the traditional inflammation imaging agents. Radiolabelled cytokines represent a reliable tool for the preclinical diagnosis of chronic inflammatory processes, even before anatomical and functional changes occur in affected tissues. Moreover, the introduction of radiolabelled monoclonal antibodies and sophisticated technique like PET/CT now make the field of inflammation imaging highly specific and accurate. In this review, different approaches of the established and experimental radiopharmaceuticals for imaging of chronic inflammation are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopharmaceuticals; Inflammation; Molecular Imaging.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600002

Influence of low environmental temperature on inflammation in bullfrog (Rana catesbeiana): qualitative and quantitative evaluation
Provedor de dados: Brazilian Jounarl of Veterinary
Autores: CATÃO-DIAS,José Luiz; SINHORINI,Idércio Luiz.
Objectives of this study were to investigate the influence of low environmental temperature on the experimentally induced inflammatory response in post-metamorphic Rana catesbeiana (bullfrogs). To accomplish these goals, 120 specimens of Rana catesbeiana were kept at 6ºC and 24ºC, and treated by transfixion of thigh muscular tissue with a 5-0 suture or IM carrageenan injection. Results obtained through qualitative and quantitative evaluations showed that the lower environmental temperature significantly modulates the inflammatory process development. The animals in both models that were kept at 6ºC showed a significantly lower number of inflammatory cells in the lesion site than the one verified at 24ºC, apart from the evolution time. On the other hand,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Temperature; Amphibia; Rana catesbeiana; Bullfrog.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000200004

Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Oliveira,Yanaihara Pinchemel Amorim de; Pontes-de-Carvalho,Lain Carlos; Couto,Ricardo David; Noronha-Dutra,Alberto Augusto.
Abstract  Background: Sepsis is an illness with a high morbidity for which no effective treatment exists. Its treatment has a high cost because it usually requires an intensive care unit and expensive antibiotics. The present study focus in the production of reactive oxygen species in the early stages of sepsis. This study aimed at investigating the production of reactive oxygen specie during the inflammatory response in patients with sepsis.  Methods: Reactive oxygen specie production and insoluble myeloperoxidase obtained from fresh whole blood were measured by photon counting chemiluminescence in the blood of 18 septic patients and 12 healthy individuals. Modified red blood cells were evaluated by staining of blood smears. The production of reactive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sepsis; Oxidative stress; Inflammation; Modified erythrocytes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100019

Helicobacter pylori eradication: influence of interleukin-1beta -31 C/T polymorphism
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Rech,Tássia Flores; Mazzoleni,Luiz Edmundo; Mazzoleni,Felipe; Francesconi,Carlos Fernando de Magalhães; Sander,Guilherme Becker; Michita,Rafael Tomoya; Nabinger,Débora Dreher; Milbradt,Tobias Cancian; Torresini,Ronaldo João Spinato; Simon,Daniel.
ABSTRACT  Aim To analyze the influence of the -31 C/T polymorphism of the interleukin-1β gene on Helicobacter pylori eradication therapy success in patients with functional dyspepsia.  Methods Functional dyspepsia was diagnosed according to the Rome III criteria. All patients underwent upper gastrointestinal endoscopy, and gastric biopsies were obtained at screening and 12 months after randomization (last follow-up visit). Urease test and histological examination were performed to define the H. pylori status. Patients received twice-daily amoxicillin, clarithromycin and omeprazole for 10 days. Genotyping of the interleukin-1beta -31 C/T polymorphism (rs1143627) was performed using polymerase chain reaction-restriction fragment length polymorphism.  Results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; Dyspepsia; Interleukin-1beta; Polymorphism; Inflammation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000400311

Preeclampsia: from epidemiological observations to molecular mechanisms
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: López-Jaramillo,P.; Casas,J.P.; Serrano,N.
Preeclampsia is the main cause of maternal mortality and is associated with a five-fold increase in perinatal mortality in developing countries. In spite of this, the etiology of preeclampsia is unknown. The present article analyzes the contradictory results of the use of calcium supplementation in the prevention of preeclampsia, and tries to give an explanation of these results. The proposal of an integrative model to explain the clinical manifestations of preeclampsia is discussed. In this proposal we suggest that preeclampsia is caused by nutritional, environmental and genetic factors that lead to the creation of an imbalance between the free radicals nitric oxide, superoxide and peroxynitrate in the vascular endothelium. The adequate interpretation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preeclampsia; Calcium supplementation; Nitric oxide; Infection; Inflammation; Oxidative stress; Endothelial dysfunction.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000001

The role of stress-activated protein kinase signaling in renal pathophysiology
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ma,F.Y.; Liu,J.; Nikolic-Paterson,D.J.
Two major stress-activated protein kinases are the p38 mitogen-activated protein kinase (MAPK) and the c-Jun amino terminal kinase (JNK). p38 and JNK are widely expressed in different cell types in various tissues and can be activated by a diverse range of stimuli. Signaling through p38 and JNK is critical for embryonic development. In adult kidney, p38 and JNK signaling is evident in a restricted pattern suggesting a normal physiological role. Marked activation of both p38 and JNK pathways occurs in human renal disease, including glomerulonephritis, diabetic nephropathy and acute renal failure. Administration of small molecule inhibitors of p38 and JNK has been shown to provide protection from renal injury in different types of experimental kidney disease...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P38; C-Jun amino terminal kinase; Inflammation; Fibrosis; Apoptosis; Renal pathophysiology.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100006

Ginsenoside Rg1 protects human renal tubular epithelial cells from lipopolysaccharide-induced apoptosis and inflammation damage
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ni,X.J.; Xu,Z.Q.; Jin,H.; Zheng,S.L.; Cai,Y.; Wang,J.J.
Ginsenoside Rg1, one of the most notable active components of Panax ginseng, has been widely reported to exert anti-inflammatory actions. This study aimed to reveal whether ginsenoside Rg1 also exhibits beneficial roles against lipopolysaccharide (LPS)-induced apoptosis and inflammation in human renal tubular epithelial cells, and to evaluate the potential role of the component on tubulointerstitial nephritis treatment. HK-2 cells were treated with various doses of ginsenoside Rg1 (0, 50, 100, 150, and 200 μM) in the absence or presence of 5 μg/mL LPS. Thereafter, CCK-8 assay, flow cytometry, western blot, migration assay, reactive oxygen species (ROS) assay, and ELISA were carried out to respectively assess cell viability, apoptosis, migration, ROS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginsenoside Rg1; LPS; Apoptosis; Inflammation; PI3K/AKT pathway; NF-κB pathway.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200608

Angiotensin-(1–7) inhibits inflammation and oxidative stress to relieve lung injury induced by chronic intermittent hypoxia in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Lu,W.; Kang,J.; Hu,K.; Tang,S.; Zhou,X.; Yu,S.; Li,Y.; Xu,L.
Obstructive sleep apnea is associated with inflammation and oxidative stress in lung tissues and can lead to metabolic abnormalities. We investigated the effects of angiotensin1–7 [Ang-(1–7)] on lung injury in rats induced by chronic intermittent hypoxia (CIH). We randomly assigned 32 male Sprague-Dawley rats (180–200 g) to normoxia control (NC), CIH-untreated (uCIH), Ang-(1–7)-treated normoxia control (N-A), and Ang-(1–7)-treated CIH (CIH-A) groups. Oxidative stress biomarkers were measured in lung tissues, and expression of NADPH oxidase 4 (Nox4) and Nox subunits (p22phox, and p47phox) was determined by Western blot and reverse transcription-polymerase chain reaction. Pulmonary pathological changes were more evident in the uCIH group than in the other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ang-(1–7); Lung injury; Obstructive sleep apnea; Inflammation; Oxidative stress; Chronic intermittent hypoxia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000603

An interleukin-33/ST2 signaling deficiency reduces overt pain-like behaviors in mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Magro,D.A.C.; Hohmann,M.S.N.; Mizokami,S.S.; Cunha,T.M.; Alves-Filho,J.C.; Casagrande,R.; Ferreira,S.H.; Liew,F.Y.; Cunha,F.Q.; Verri Jr,W.A.
Interleukin (IL)-33, the most recent member of the IL family of cytokines, signals through the ST2 receptor. IL-33/ST2 signaling mediates antigen challenge-induced mechanical hyperalgesia in the joints and cutaneous tissues of immunized mice. The present study asked whether IL-33/ST2 signaling is relevant to overt pain-like behaviors in mice. Acetic acid and phenyl-p-benzoquinone induced significant writhing responses in wild-type (WT) mice; this overt nociceptive behavior was reduced in ST2-deficient mice. In an antigen-challenge model, ST2-deficient immunized mice had reduced induced flinch and licking overt pain-like behaviors. In the formalin test, ST2-deficient mice also presented reduced flinch and licking responses, compared with WT mice. Naive WT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interleukin-33; ST2; Pain; Nociception; Inflammation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700601

Edaravone protects endotoxin-induced liver injury by inhibiting apoptosis and reducing proinflammatory cytokines
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zong,L.; Yu,Q.H.; Du,Y.X.; Deng,X.M.
Studies have shown that edaravone may prevent liver injury. This study aimed to investigate the effects of edaravone on the liver injury induced by D-galactosamine (GalN) and lipopolysaccharide (LPS) in female BALB/c mice. Edaravone was injected into mice 30 min before and 4 h after GalN/LPS injection. The survival rate was determined within the first 24 h. Animals were killed 8 h after GalN/LPS injection, and liver injury was biochemically and histologically assessed. Hepatocyte apoptosis was measured by TUNEL staining; proinflammatory cytokines [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)] in the liver were assayed by ELISA; expression of caspase-8 and caspase-3 proteins was detected by Western blot assay; and caspase-3 activity was also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Edaravone; Apoptosis; Endotoxin; Liver injury; Inflammation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300231

Effects of single-dose atorvastatin on interleukin-6, interferon gamma, and myocardial no-reflow in a rabbit model of acute myocardial infarction and reperfusion
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhao,X.J.; Liu,X.L.; He,G.X.; Xu,H.P.
The mechanisms of statins relieving the no-reflow phenomenon and the effects of single-dose statins on it are not well known. This study sought to investigate the effects of inflammation on the no-reflow phenomenon in a rabbit model of acute myocardial infarction and reperfusion (AMI/R) and to evaluate the effects of single-dose atorvastatin on inflammation and myocardial no-reflow. Twenty-four New Zealand white male rabbits (5-6 months old) were randomized to three groups of eight: a sham-operated group, an AMI/R group, and an atorvastatin-treated group (10 mg/kg). Animals in the latter two groups were subjected to 4 h of coronary occlusion followed by 2 h of reperfusion. Serum levels of interleukin (IL)-6 were measured by enzyme-linked immunosorbent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial infarction; No-reflow phenomenon; Inflammation; Atorvastatin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300245

Dialysis water treated by reverse osmosis decreases the levels of C-reactive protein in uremic patients
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Thomé,F.S.; Senger,M.; Garcez,C.; Garcez,J.; Chemello,C.; Manfro,R.C.
Atherosclerosis is a major complication of chronic renal failure. Microinflammation is involved in atherogenesis and is associated with uremia and dialysis. The role of dialysate water contamination in inducing inflammation has been debated. Our aim was to study inflammatory markers in patients on chronic dialysis, before and 3 to 6 months after switching the water purification system from deionization to reverse osmosis. Patients had demographic, clinical and nutritional information collected and blood drawn for determination of albumin, ferritin, C-reactive protein (CRP), interleukin-6, and tumor necrosis factor-alpha in both situations. Acceptable levels of water purity were less than 200 colony-forming units of bacteria and less than 1 ng/ml of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemodialysis; C-reactive protein; Dialysate water; Reverse osmosi; Inflammation; Uremia.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500018

Low-dose doxycycline prevents inflammatory bone resorption in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bezerra,M.M.; Brito,G.A.C.; Ribeiro,R.A.; Rocha,F.A.C.
Matrix metalloproteinases (MMP) are considered to be key initiators of collagen degradation, thus contributing to bone resorption in inflammatory diseases. We determined whether subantimicrobial doses of doxycycline (DX) (&lt;=10 mg kg-1 day-1), a known MMP inhibitor, could inhibit bone resorption in an experimental periodontitis model. Thirty male Wistar rats (180-200 g) were subjected to placement of a nylon thread ligature around the maxillary molars and sacrificed after 7 days. Alveolar bone loss (ABL) was measured macroscopically in one hemiarcade and the contralateral hemiarcade was processed for histopathologic analysis. Groups of six animals each were treated with DX (2.5, 5 or 10 mg kg-1 day-1, sc, 7 days) and compared to nontreated (NT) rats. NT...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tetracyclines; Doxycycline; Metalloproteinases; Periodontitis; Osteoporosis; Bone resorption; Inflammation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500015

Correlates of C-reactive protein levels in young adults: a population-based cohort study of 3827 subjects in Brazil
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Nazmi,A.; Oliveira,I.O.; Victora,C.G.
The socio-demographic, behavioral and anthropometric correlates of C-reactive protein levels were examined in a representative young adult Brazilian population. The 1982 Pelotas Birth Cohort Study (Brazil) recruited over 99% of births in the city of Pelotas that year (N = 5914). Individuals belonging to the cohort have been prospectively followed up. In 2004-2005, 77.4% of the cohort was traced, members were interviewed and 3827 individuals donated blood. Analyses of the outcome were based on a conceptual model that differentiated confounders from potential mediators. The following independent variables were studied in relation to levels of C-reactive protein in sex-stratified analyses: skin color, age, family income, education, parity, body mass index,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-reactive protein; Inflammation; Cohort studies; Prospective studies; Brazil.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500003

Evidence for the participation of nitric oxide in pemphigus
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Siebra,M.X.; Santos,M.A.; Almeida,T.L.P.; Leite,A.C.R.M.; Cunha,F.Q.; Rocha,F.A.C.
Pemphigus is an inflammatory autoimmune disorder of the skin. Nitric oxide (NO) is an inflammatory mediator linked to a variety of physiological and pathophysiological phenomena that include skin tumors, psoriasis, urticaria, and atopic dermatitis. Inflammatory cells present in pemphigus lesions are important sources of NO production. We investigated whether NO is involved in pemphigus. A prospective cohort study was conducted at the Dermatology Service of the Hospital Universitário Walter Cantídio of the Federal University of Ceará. All patients seen at the outpatient clinic between August 2000 and July 2001, with a clinically and histologically confirmed diagnosis of pemphigus were included. The median age was 42.5 years (range: 12-69 years) with a male...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Autoimmunity; Inflammation; Neutrophils; Nitric oxide; Pemphigus; Inducible nitric oxide synthase.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500014

Effect of eccentric training on mitochondrial function and oxidative stress in the skeletal muscle of rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Silva,L.A.; Bom,K.F.; Tromm,C.B.; Rosa,G.L.; Mariano,I.; Pozzi,B.G.; Tuon,T.; Stresck,E.L.; Souza,C.T.; Pinho,R.A.
The objective of the present study was to investigate the effects of eccentric training on the activity of mitochondrial respiratory chain enzymes, oxidative stress, muscle damage, and inflammation of skeletal muscle. Eighteen male mice (CF1) weighing 30-35 g were randomly divided into 3 groups (N = 6): untrained, trained eccentric running (16°; TER), and trained running (0°) (TR), and were submitted to an 8-week training program. TER increased muscle oxidative capacity (succinate dehydrogenase and complexes I and II) in a manner similar to TR, and TER did not decrease oxidative damage (xylenol and creatine phosphate) but increased antioxidant enzyme activity (superoxide dismutase and catalase) similar to TR. Muscle damage (creatine kinase) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eccentric training; Mitochondrial enzyme activity; Oxidative stress; Muscle damage; Inflammation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100014

Neutrophil function and metabolism in individuals with diabetes mellitus
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Alba-Loureiro,T.C.; Munhoz,C.D.; Martins,J.O.; Cerchiaro,G.A.; Scavone,C.; Curi,R.; Sannomiya,P.
Neutrophils act as first-line-of-defense cells and the reduction of their functional activity contributes to the high susceptibilityto and severity of infections in diabetes mellitus. Clinical investigations in diabetic patients and experimental studies in diabetic rats and mice clearly demonstrated consistent defects of neutrophil chemotactic, phagocytic and microbicidal activities. Other alterations that have been reported to occur during inflammation in diabetes mellitus include: decreased microvascular responses to inflammatory mediators such as histamine and bradykinin, reduced protein leakage and edema formation, reduced mast cell degranulation, impairment of neutrophil adhesionto the endothelium and migration to the site of inflammation, production...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutrophils; Inflammation; Diabetes mellitus; Insulin; Metabolism and cellular function.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800003

Side effects of oxysterols: cytotoxicity, oxidation, inflammation, and phospholipidosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Vejux,A.; Malvitte,L.; Lizard,G.
Oxysterols are 27-carbon atom molecules resulting from autoxidation or enzymatic oxidation of cholesterol. They are present in numerous foodstuffs and have been demonstrated to be present at increased levels in the plasma of patients with cardiovascular diseases and in atherosclerotic lesions. Thus, their role in lipid disorders is widely suspected, and they might also be involved in important degenerative diseases such as Alzheimer's disease, osteoporosis, and age-related macular degeneration. Since atherosclerosis is associated with the presence of apoptotic cells and with oxidative and inflammatory processes, the ability of some oxysterols, especially 7-ketocholesterol and 7β-hydroxycholesterol, to trigger cell death, activate inflammation, and modulate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Atherosclerosis; Inflammation; Oxysterols; Phospholipidosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700001

Epigallocatechin-3-gallate ameliorates lipopolysaccharide-induced acute lung injury by suppression of TLR4/NF-κB signaling activation
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Wang,Jia; Fan,Shi Ming; Zhang,Jiong.
Acute lung injury (ALI) is a serious clinical syndrome with a high rate of mortality. The activation of inflammation is well-recognized as a vital factor in the pathogenesis of lipopolysaccharide (LPS)-induced ALI. Therefore, suppression of the inflammatory response could be an ideal strategy to prevent ALI. Epigallocatechin-3-gallate (EGCG), mainly from green tea, has been shown to have an anti-inflammatory effect. The aim of the study was to explore whether EGCG alleviates inflammation in sepsis-related ALI. Male BALB/C mice were treated with EGCG (10 mg/kg) intraperitoneally (ip) 1 h before LPS injection (10 mg/kg, ip). The results showed that EGCG attenuated LPS-induced ALI as it decreased the changes in blood gases and reduced the histological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epigallocatechin-3-gallate; Acute lung injury; Inflammation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700605

Chronic granulomatous disease: why an inflammatory disease?
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Roxo-Junior,P.; Simão,H.M.L.
Chronic granulomatous disease is a primary immunodeficiency caused by mutations in the genes encoding subunits of the phagocytic NADPH oxidase system. Patients can present with severe, recurrent infections and noninfectious conditions. Among the latter, inflammatory manifestations are predominant, especially granulomas and colitis. In this article, we systematically review the possible mechanisms of hyperinflammation in this rare primary immunodeficiency condition and their correlations with clinical aspects.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic granulomatous disease; Inflammation; Infection; Granulomas; NADPH oxidase.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100924

Blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in patients with symptoms suggesting reactive hypoglycemia
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Eik Filho,W.; Marcon,S.S.; Krupek,T.; Previdelli,I.T.S.; Pereira,O.C.N.; Silva,M.A.R.C.P.; Bazotte,R.B.
We evaluated the impact of postprandial glycemia on blood levels of pro-inflammatory and anti-inflammatory cytokines during an oral glucose tolerance test in non-diabetic patients with symptoms suggesting reactive hypoglycemia. Eleven patients with clinical symptoms suggesting reactive hypoglycemia received an oral glucose solution (75 g) Blood was collected at 0 (baseline), 30, 60, 120 and 180 min after glucose ingestion and the plasma concentrations of interferon-α (IFN-α), interferon-γ (IFN-γ), interleukin-1 receptor antagonist (IL-1RA), interleukin 2 (IL-2), interleukin-2 receptor (IL-2R), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 10 (IL-10), interleukin-12 (IL-12), interleukin 13 (IL-13), interleukin 15 (IL-15),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pro-inflammatory cytokines; Anti-inflammatory cytokines; Glucose tolerance test; Inflammation; Postprandial glycemia.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800702

LXW7 ameliorates focal cerebral ischemia injury and attenuates inflammatory responses in activated microglia in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Fang,T.; Zhou,D.; Lu,L.; Tong,X.; Wu,J.; Yi,L.
Inflammation plays a pivotal role in ischemic stroke, when activated microglia release excessive pro-inflammatory mediators. The inhibition of integrin αvβ3 improves outcomes in rat focal cerebral ischemia models. However, the mechanisms by which microglia are neuroprotective remain unclear. This study evaluated whether post-ischemic treatment with another integrin αvβ3 inhibitor, the cyclic arginine-glycine-aspartic acid (RGD) peptide-cGRGDdvc (LXW7), alleviates cerebral ischemic injury. The anti-inflammatory effect of LXW7 in activated microglia within rat focal cerebral ischemia models was examined. A total of 108 Sprague-Dawley rats (250–280 g) were subjected to middle cerebral artery occlusion (MCAO). After 2 h, the rats were given an intravenous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Activated microglia; Ischemic stroke; Integrin αvβ3; TNF-α.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900602

Lung hyperinflation stimulates the release of inflammatory mediators in spontaneously breathing subjects
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Malbouisson,L.M.S.; Szeles,T.F.; Barbalho,L.; Massoco,C.O.; Carmona,M.J.C.; Carvalho,C.R.R.; Pelosi,P.; Auler Jr.,J.O.C.
Lung hyperinflation up to vital capacity is used to re-expand collapsed lung areas and to improve gas exchange during general anesthesia. However, it may induce inflammation in normal lungs. The objective of this study was to evaluate the effects of a lung hyperinflation maneuver (LHM) on plasma cytokine release in 10 healthy subjects (age: 26.1 ± 1.2 years, BMI: 23.8 ± 3.6 kg/m²). LHM was performed applying continuous positive airway pressure (CPAP) with a face mask, increased by 3-cmH2O steps up to 20 cmH2O every 5 breaths. At CPAP 20 cmH2O, an inspiratory pressure of 20 cmH2O above CPAP was applied, reaching an airway pressure of 40 cmH2O for 10 breaths. CPAP was then decreased stepwise. Blood samples were collected before and 2 and 12 h after LHM....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung hyperinflation; Ventilator-induced lung injury; Inflammation; Cytokines.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200011

Ventilation with high tidal volume induces inflammatory lung injury
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bueno,P.C.S.; Bueno,C.E.; Santos,M.L.; Oliveira-Júnior,I.; Salomão,R.; Pinheiro,B.V.; Beppu,O.S.
Mechanical ventilation with high tidal volumes (V T) has been shown to induce lung injury. We examined the hypothesis that this procedure induces lung injury with inflammatory features. Anesthetized male Wistar rats were randomized into three groups: group 1 (N = 12): V T = 7 ml/kg, respiratory rate (RR) = 50 breaths/min; group 2 (N = 10): V T = 21 ml/kg, RR = 16 breaths/min; group 3 (N = 11): V T = 42 ml/kg, RR = 8 breaths/min. The animals were ventilated with fraction of inspired oxygen of 1 and positive end-expiratory pressure of 2 cmH2O. After 4 h of ventilation, group 3, compared to groups 1 and 2, had lower PaO2 [280 (range 73-458) vs 517 (range 307-596), and 547 mmHg (range 330-662), respectively, P&lt;0.05], higher wet lung weight [3.62 ± 0.91 vs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lung injury; Mechanical ventilation; Inflammation; TNF-alpha.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200007

Inflammation induced by increased frequency of intermittent hypoxia is attenuated by tempol administration
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,J.; Zheng,L.; Cao,J.; Chen,B.; Jin,D.
The levels of serum inflammatory cytokines and the activation of nuclear factor kappa B (NF-κB) and hypoxia inducible factor-1α (HIF-1α) in heart tissues in response to different frequencies of intermittent hypoxia (IH) and the antioxidant tempol were evaluated. Wistar rats (64 males, 200-220 g) were randomly divided into 6 experimental groups and 2 control groups. Four groups were exposed to IH 10, 20, 30, or 40 times/h. The other 2 experimental groups were challenged with IH (30 times/h) plus tempol, either beginning on day 0 (IH30T0) or on day 29 (IH30T29). After 6 weeks of challenge, serum levels of tumor necrosis factor (TNF)-α, intracellular adhesion molecule (ICAM)-1, and interleukin-10 were measured, and western blot analysis was used to detect...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obstructive sleep apnea; Intermittent hypoxia; Inflammation; Nuclear factor kappa B; Antioxidant.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201115

Inflammatory biomarkers responses after acute whole body vibration in fibromyalgia
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ribeiro,V.G.C.; Mendonça,V.A.; Souza,A.L.C.; Fonseca,S.F.; Camargos,A.C.R.; Lage,V.K.S.; Neves,C.D.C.; Santos,J.M.; Teixeira,L.A.C.; Vieira,E.L.M.; Teixeira Junior,A.L.; Mezêncio,B.; Fernandes,J.S.C.; Leite,H.R.; Poortmans,J.R.; Lacerda,A.C.R.
The aims of this study were 1) to characterize the intensity of the vibration stimulation in women diagnosed with fibromyalgia (FM) compared to a control group of healthy women (HW) matched by age and anthropometric parameters, and 2) to investigate the effect of a single session of whole body vibration (WBV) on inflammatory responses. Levels of adipokines, soluble tumor necrosis factor receptors (sTNFr1, sTNFr2), and brain-derived neurotrophic factor (BDNF) were determined by enzyme-linked immunosorbent assay. Oxygen consumption (VO2) was estimated by a portable gas analysis system, heart rate (HR) was measured using a HR monitor, and perceived exertion (RPE) was evaluated using the Borg scale of perceived exertion. Acutely mild WBV increased VO2 and HR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomarkers; Fibromyalgia; Clinical trial; Inflammation; Acute exercise.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400615

Hydrogen sulfide in posthemorrhagic shock mesenteric lymph drainage alleviates kidney injury in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Han,B.; Zhao,Z.G.; Zhang,L.M.; Li,S.G.; Niu,C.Y.
Posthemorrhagic shock mesenteric lymph (PHSML) is a key factor in multiple organ injury following hemorrhagic shock. We investigated the role of hydrogen sulfide (H2S) in PHSML drainage in alleviating acute kidney injury (AKI) by administering D,L-propargylglycine (PPG) and sodium hydrosulfide hydrate (NaHS) to 12 specific pathogen-free male Wistar rats with PHSML drainage. A hemorrhagic shock model was established in 4 experimental groups: shock, shock+drainage, shock+drainage+PPG (45 mg/kg, 0.5 h prehemorrhage), and shock+drainage+NaHS (28 µmol/kg, 0.5 h prehemorrhage). Fluid resuscitation was performed after 1 h of hypotension, and PHMSL was drained in the last three groups for 3 h after resuscitation. Renal function and histomorphology were assessed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemorrhagic shock; Mesenteric lymph; Drainage; Kidney injury; Hydrogen sulfide; Inflammation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700622

Relationship of inflammatory markers and pain in patients with head and neck cancer prior to anticancer therapy
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Oliveira,K.G.; von Zeidler,S.V.; Lamas,A.Z.; de Podestá,J.R.V.; Sena,A.; Souza,E.D.; Lenzi,J.; Lemos,E.M.; Gouvea,S.A.; Bissoli,N.S.
Pain is a common symptom in patients with cancer, including those with head and neck cancer (HNC). While studies suggest an association between chronic inflammation and pain, levels of inflammatory cytokines, such as C-reactive protein (CRP) and tumor necrosis factor-alpha (TNF-α), have not been correlated with pain in HNC patients who are not currently undergoing anticancer treatment. The purpose of this study was to examine the relationship between these inflammatory markers and perceived pain in HNC patients prior to anticancer therapy. The study group consisted of 127 HNC patients and 9 healthy controls. Pain was assessed using the Brief Pain Inventory (BPI), and serum levels of CRP and TNF-α were determined using the particle-enhanced turbidimetric...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pain; Head and neck cancer; C-reactive protein; Tumor necrosis factor-alpha; Inflammation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700600

Inflammatory and anti-inflammatory effects of soybean agglutinin
Provedor de dados: Brazilian Journal of Medical and Biological Research
Soybean agglutinin (SBA) lectin, a protein present in raw soybean meals, can bind to and be extensively endocytosed by intestinal epithelial cells, being nutritionally toxic for most animals. In the present study we show that SBA (5-200 µg/cavity) injected into different cavities of rats induced a typical inflammatory response characterized by dose-dependent exudation and neutrophil migration 4 h after injection. This effect was blocked by pretreatment with glucocorticoid (0.5 mg/kg) or by co-injection of N-acetyl-galactosamine (100 x [M] lectin), but not of other sugars (100 x [M] lectin), suggesting an inflammatory response related to the lectin activity. Neutrophil accumulation was not dependent on a direct effect of SBA on the macrophage population...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lectin; Soybean agglutinin (SBA); Neutrophil migration; Inflammation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700009

Trans fatty acid intake is associated with insulin sensitivity but independently of inflammation
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Angelieri,C.T.; Barros,C.R.; Siqueira-Catania,A.; Ferreira,S.R.G.
High saturated and trans fatty acid intake, the typical dietary pattern of Western populations, favors a proinflammatory status that contributes to generating insulin resistance (IR). We examined whether the consumption of these fatty acids was associated with IR and inflammatory markers. In this cross-sectional study, 127 non-diabetic individuals were allocated to a group without IR and 56 to another with IR, defined as homeostasis model assessment-IR (HOMA-IR) &amp;gt;2.71. Diet was assessed using 24-h food recalls. Multiple linear regression was employed to test independent associations with HOMA-IR. The IR group presented worse anthropometric, biochemical and inflammatory profiles. Energy intake was correlated with abdominal circumference and inversely...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin resistance; Inflammation; Fatty acids.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700009

The hepatic clearance of recombinant tissue-type plasminogen activator decreases after an inflammatory stimulus
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Nagaoka,M.R.; Kouyoumdjian,M.; Borges,D.R.
We have shown that tissue-type plasminogen activator (tPA) and plasma kallikrein share a common pathway for liver clearance and that the hepatic clearance rate of plasma kallikrein increases during the acute-phase (AP) response. We now report the clearance of tPA from the circulation and by the isolated, exsanguinated and in situ perfused rat liver during the AP response (48-h ex-turpentine treatment). For the sake of comparison, the hepatic clearance of a tissue kallikrein and thrombin was also studied. We verified that, in vivo, the clearance of 125I-tPA from the circulation of turpentine-treated rats (2.2 ± 0.2 ml/min, N = 7) decreases significantly (P = 0.016) when compared to normal rats (3.2 ± 0.3 ml/min, N = 6). The AP response does not modify the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Liver clearance; Inflammation; Kallikrein; Thrombin; Tissue-type plasminogen activator.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100016

Effect of fatty acids on leukocyte function
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pompéia,C.; Lopes,L.R.; Miyasaka,C.K.; Procópio,J.; Sannomiya,P.; Curi,R.
Fatty acids have various effects on immune and inflammatory responses, acting as intracellular and intercellular mediators. Polyunsaturated fatty acids (PUFAs) of the omega-3 family have overall suppressive effects, inhibiting lymphocyte proliferation, antibody and cytokine production, adhesion molecule expression, natural killer cell activity and triggering cell death. The omega-6 PUFAs have both inhibitory and stimulatory effects. The most studied of these is arachidonic acid that can be oxidized to eicosanoids, such as prostaglandins, leukotrienes and thromboxanes, all of which are potent mediators of inflammation. Nevertheless, it has been found that many of the effects of PUFA on immune and inflammatory responses are not dependent on eicosanoid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fatty acids; Immune system; Inflammation; Dietary lipids; Leukocytes.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100001

Pentoxifylline decreases tumor necrosis factor and interleukin-1 during high tidal volume
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Oliveira-Junior,I.S.; Pinheiro,B.V.; Silva,I.D.C.G.; Salomão,R.; Zollner,R.L.; Beppu,O.S.
Tumor necrosis factor-alpha (TNF-alpha) is one of the most important proinflammatory cytokines which plays a central role in host defense and in the acute inflammatory response related to tissue injury. The major source of TNF-alpha are immune cells such as neutrophils and macrophages. We tested the hypothesis that pentoxifylline, a methylxanthine derivative, down-regulates proinflammatory cytokine expression during acute lung injury in rats. Male Wistar rats weighing 250 to 450 g were anesthetized ip with 50 mg/kg sodium thiopental and randomly divided into three groups: group 1 (N = 7): tidal volume (V T) = 7 ml/kg, respiratory rate (RR) = 50 breaths/min and normal saline infusion; group 2 (N = 7): V T = 42 ml/kg, RR = 9 breaths/min and normal saline...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tumor necrosis factor-alpha; Pentoxifylline; Inflammation; Acute lung injury.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000011

Quality of life on hemodialysis and inflammation: a descriptive analysis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bacci,M.R.; Adami,F.; Figueiredo,F.W.S.; Alves,B.C.A.; da Veiga,G.L.; Fonseca,F.L.A.
Chronic kidney disease (CKD) is highly prevalent worldwide. Patients with CKD on hemodialysis are more likely to present behavioral changes and worse quality of life as a result of their routine and complications. They also have higher levels of cytokines. The aim of this study is to assess the relationship between the inflammatory profile and quality of life measured by KDOQL-SF36 in hemodialysis outpatients. Patients older than 21 years of age and on routine hemodialysis for at least 6 months with treatment on a regular weekly basis were included and their anthropometric parameters and serum inflammatory markers were evaluated. Thirty patients consented to participate. Homocysteine (Hcy) levels were correlated with worse glomerular filtration rate (GFR;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quality of life; Hemodialysis; Inflammation; Homocysteine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600608

Dermatan sulfate reduces monocyte chemoattractant protein 1 and TGF-β production, as well as macrophage recruitment and myofibroblast accumulation in mice with unilateral ureteral obstruction
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Belmiro,C.L.R.; Gonçalves,R.G.; Kozlowski,E.O.; Werneck,A.F.; Takyia,C.M.; Leite-Jr.,M.; Pavão,M.S.G.
Selectins play an essential role in most inflammatory reactions, mediating the initial leukocyte-rolling event on activated endothelium. Heparin and dermatan sulfate (DS) bind and block P- and L-selectin function in vitro. Recently, we reported that subcutaneous administration of DS inhibits colon inflammation in rats by reducing macrophage and T-cell recruitment and macrophage activation. In the present study, we examined the effect of porcine intestinal mucosa DS on renal inflammation and fibrosis in mice after unilateral ureteral obstruction (UUO). Twenty-four adult male Swiss mice weighing 20-25 g were divided into 4 groups: group C (N = 6) was not subjected to any surgical manipulation; group SH (N = 6) was subjected to surgical manipulation but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycosaminoglycans; Dermatan sulfate; Kidney; Inflammation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700003

The inflammatory stimulus of a natural latex biomembrane improves healing in mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Andrade,T.A.M.; Iyer,A.; Das,P.K.; Foss,N.T.; Garcia,S.B.; Coutinho-Netto,J.; Jordão-Jr.,A.A.; Frade,M.A.C.
The aim of the present study was to compare healing obtained with biomembranes with the natural healing process (sham) using biochemical and immunohistological assays. C57BL/6 mice were divided into 4 groups of 15 mice each and received different subcutaneous implants: natural latex biomembrane (NLB), denatured latex (DL), expanded polytetrafluorethylene (ePTFE), or sham. On the 2nd, 7th, and 14th days post-treatment, 5 mice per group were sacrificed and biopsied for the following measurements: oxidative stress based on malondialdehyde (MDA), myeloperoxidase (MPO) and hydrogen peroxide by the method of ferrous oxidation-xylenol orange (FOX), as well as glutathione and total proteins; histological evaluation to enumerate inflammatory cells, fibroblasts,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wound healing; Inflammation; Oxidative stress; Angiogenesis; Latex; Histomorphometry.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000009

TNF-a and IL-10 levels in tracheobronchial lavage of ventilated preterm infants and subsequent lung function
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Corso,A.L.; Pitrez,P.M.C.; Machado,D.C.; Stein,R.T.; Jones,M.H.
The role of airway inflammation in ventilated preterm newborns and the risk factors associated with the development of chronic lung disease are not well understood. Our objective was to analyze the association of the airway inflammatory response in ventilated preterm infants by serial measurements of TNF-a and IL-10 in tracheobronchial lavage (TBL) with perinatal factors and lung function measured early in life. A series of TBL samples were collected from ventilated preterm infants (less than 32 weeks of gestational age) and concentrations of TNF-a and IL-10 were measured by ELISA. Pulmonary function tests were performed after discharge by the raised volume rapid compression technique. Twenty-five subjects were recruited and 70 TBL samples were obtained....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Newborn; Chronic lung disease; Interleukin-10; Tumor necrosis factor-a; Prematurity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400016

Mechanisms in anti-inflammation and resolution: the role of lipoxins and aspirin-triggered lipoxins
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Fierro,I.M.; Serhan,C.N.
Multicellular host responses to infection, injury or inflammatory stimuli lead to the formation of a broad range of chemical mediators by the host. The integrated response of the host is essential to health and disease; thus it is important to achieve a more complete understanding of the molecular and cellular events governing the formation and actions of endogenous mediators of resolution that appear to control the duration of inflammation. Lipoxins are trihydroxytetraene-containing lipid mediators that can be formed during cell-cell interactions and are predominantly counterregulators of some well-known mediators of inflammation. Since this circuit of lipoxin formation and action appears to be of physiological relevance for the resolution of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Lipoxins; Resolution.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500002

[RETRACTED ARTICLE] Ginsenoside Rd inhibits IL-1β-induced inflammation and degradation of intervertebral disc chondrocytes by increasing IL1RAP ubiquitination
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,Wei-Jia; Liu,Ying; Wei,Jie-Shu; Wu,Ya-Li.
Many compounds of ginsenosides show anti-inflammatory properties. However, their anti-inflammatory effects in intervertebral chondrocytes in the presence of inflammatory factors have never been shown. Increased levels of pro-inflammatory cytokines are generally associated with the degradation and death of chondrocytes; therefore, finding an effective and nontoxic substance that attenuates the inflammation is worthwhile. In this study, chondrocytes were isolated from the nucleus pulposus tissues, and the cells were treated with ginsenoside compounds and IL-1β, alone and in combination. Cell viability and death rate were assessed by CCK-8 and flow cytometry methods, respectively. PCR, western blot, and immunoprecipitation assays were performed to determine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginsenoside Rd; IL-1β; Inflammation; Degradation; Intervertebral disc chondrocytes; IL1RAP ubiquitination.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900602

Association of terpinolene and diclofenac presents antinociceptive and anti-inflammatory synergistic effects in a model of chronic inflammation
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Macedo,E.M.A.; Santos,W.C.; Sousa Neto,B.P.; Lopes,E.M.; Piauilino,C.A.; Cunha,F.V.M.; Sousa,D.P.; Oliveira,F.A.; Almeida,F.R.C.
Pharmacological treatment of inflammatory pain is usually done by administration of non-steroidal anti-inflammatory drugs (NSAIDs). These drugs present high efficacy, although side effects are common, especially gastrointestinal lesions. One of the pharmacological strategies to minimize such effects is the combination of drugs and natural products with synergistic analgesic effect. The monoterpene terpinolene (TPL) is a chemical constituent of essential oils present in many plant species, which have pharmacological activities, such as analgesic and anti-inflammatory. The association of ineffective doses of TPL and diclofenac (DCF) (3.125 and 1.25 mg/kg po, respectively) presented antinociceptive and anti-inflammatory effects in the acute (0, 1, 2, 3, 4, 5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Terpinolene; Inflammation; Antinociception; Pharmacological synergism.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700602

Enhanced mucosal re-epithelialization induced by short chain fatty acids in experimental colitis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Aguilar-Nascimento,J.E.; França-da-Silva,L.R.; de-Oliveira,A.F.; Gomes-da-Silva,M.H.
The short chain fatty acids (SCFA) are the best nutrients for the colonocytes. Glucose is poorly used as a fuel but may be transformed into SCFA by colonic bacteria. The aim of this study was to investigate the effect of SCFA or glucose on experimental colitis. Colitis was induced in 30 Wistar rats by colonic instillation of 4% acetic acid. Five days later they were randomized to receive twice a day colonic lavage containing saline (controls, N = 10), 10% hypertonic glucose (N = 10) or SCFA (N = 10) until day 8 when they were killed. At autopsy, the colon was removed and weighed and the mucosa was evaluated macro- and microscopically and stripped out for DNA assay. Data are reported as mean ± SD or median [range] as appropriate. All animals lost weight but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Short chain fatty acids; Ulcerative colitis; Glucose; Colon; Inflammation; Mucosa.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800005

Is persistent hypotension after transient cardiogenic shock associated with an inflammatory response?
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Park,M.; A.T.,Maciel; Noritomi,D.T.; Brunialti,M.K.; Salomão,R.; Schettino,G.P.P.; Azevedo,L.C.P.
We evaluated the recovery of cardiovascular function after transient cardiogenic shock. Cardiac tamponade was performed for 1 h and post-shock data were collected in 5 domestic large white female pigs (43 ± 5 kg) for 6 h. The control group (N = 5) was observed for 6 h after 1 h of resting. During 1 h of cardiac tamponade, experimental animals evolved a low perfusion status with a higher lactate level (8.0 ± 2.2 vs 1.9 ± 0.9 mEq/L), lower standard base excess (-7.3 ± 3.3 vs 2.0 ± 0.9 mEq/L), lower urinary output (0.9 ± 0.9 vs 3.0 ± 1.4 mL·kg-1·h-1), lower mixed venous saturation, higher ileum partial pressure of CO2-end tidal CO2 (EtCO2) gap and a lower cardiac index than the control group. Throughout the 6-h recovery phase after cardiac tamponade,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiogenic shock; Cardiac tamponade; Systemic inflammatory response syndrome; Swine; Myocardial dysfunction; Inflammation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800002

Cytokine profile and lymphocyte subsets in type 2 diabetes
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Francisco,C.O.; Catai,A.M.; Moura-Tonello,S.C.G.; Arruda,L.C.M.; Lopes,S.L.B.; Benze,B.G.; Del Vale,A.M.; Malmegrim,K.C.R.; Leal,A.M.O.
Type 2 diabetes mellitus (T2D) is a metabolic disease with inflammation as an important pathogenic background. However, the pattern of immune cell subsets and the cytokine profile associated with development of T2D are unclear. The objective of this study was to evaluate different components of the immune system in T2D patients' peripheral blood by quantifying the frequency of lymphocyte subsets and intracellular pro- and anti-inflammatory cytokine production by T cells. Clinical data and blood samples were collected from 22 men (51.6±6.3 years old) with T2D and 20 nonsmoking men (49.4±7.6 years old) who were matched for age and sex as control subjects. Glycated hemoglobin, high-sensitivity C-reactive protein concentrations, and the lipid profile were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes mellitus; Cytokines; Lymphocytes; Inflammation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400707

Nitro-fatty acids: novel anti-inflammatory lipid mediators
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Rubbo,H.
Nitro-fatty acids are formed and detected in human plasma, cell membranes, and tissue, modulating metabolic as well as inflammatory signaling pathways. Here we discuss the mechanisms of nitro-fatty acid formation as well as their key chemical and biochemical properties. The electrophilic properties of nitro-fatty acids to activate anti-inflammatory signaling pathways are discussed in detail. A critical issue is the influence of nitroarachidonic acid on prostaglandin endoperoxide H synthases, redirecting arachidonic acid metabolism and signaling. We also analyze in vivo data supporting nitro-fatty acids as promising pharmacological tools to prevent inflammatory diseases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitro-fatty acids; Antioxidants; Nitric oxide; Inflammation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900728

Increased expression of p38 mitogen- activated protein kinase is related to the acute renal lesions induced by gentamicin
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Volpini,R.A.; Balbi,A.P.C.; Costa,R.S.; Coimbra,T.M.
Mitogen-activated protein kinases (MAPK) may be involved in the pathogenesis of acute renal failure. This study investigated the expression of p-p38 MAPK and nuclear factor kappa B (NF-kappaB) in the renal cortex of rats treated with gentamicin. Twenty rats were injected with gentamicin, 40 mg/kg, im, twice a day for 9 days, 20 with gentamicin + pyrrolidine dithiocarbamate (PDTC, an NF-kappaB inhibitor), 14 with 0.15 M NaCl, im, twice a day for 9 days, and 14 with 0.15 M NaCl , im, twice a day for 9 days and PDTC, 50 mg kg-1 day-1, ip, twice a day for 15 days. The animals were killed 5 and 30 days after the last of the injections and the kidneys were removed for histological, immunohistochemical and Western blot analysis and for nitrate determination. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: P38 mitogen-activated protein kinase; Nuclear factor-kappaB; Inflammation; Renal fibrosis; Gentamicin-induced acute tubular necrosis; Nitric oxide.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600016

The anti-IRBP IgG1 and IgG2a response does not correlate with susceptibility to experimental autoimmune uveitis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Vieira de Moraes,L.; Martins,G.A.; Flangini,M.; Ibañez,O.M.; Sant'Anna,O.A.; Rizzo,L.V.
Susceptibility to experimental autoimmune uveitis (EAU) in inbred mice has been associated with a dominant Th1 response. Elevated anti-inter-photoreceptor retinoid-binding protein (anti-IRBP) IgG2a/IgG1 antibody ratios have been implicated as candidate markers to predict disease severity. In the present study, both the anti-IRBP antibody isotype and severity of EAU phenotypes were examined in 4 non-isogenic genetically selected mouse lines to determine if they can be used as general markers of disease. Mice between 8 and 12 weeks old selected for high (H III) or low (L III) antibody response and for maximum (AIR MAX) or minimum (AIR MIN) acute inflammatory reaction (AIR) were immunized with IRBP. Each experiment was performed with at least 5 mice per...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autoimmunity; IgG1 and IgG2a isotypes; Inflammation; Genetically selected mice; Uveitis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600010

Chemokines in the cerebrospinal fluid of patients with active and stable relapsing-remitting multiple sclerosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Moreira,M.A.; Souza,A.L.S.; Lana-Peixoto,M.A.; Teixeira,M.M.; Teixeira,A.L.
Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the human central nervous system. Although its etiology is unknown, the accumulation and activation of mononuclear cells in the central nervous system are crucial to its pathogenesis. Chemokines have been proposed to play a major role in the recruitment and activation of leukocytes in inflammatory sites. They are divided into subfamilies on the basis of the location of conserved cysteine residues. We determined the levels of some CC and CXC chemokines in the cerebrospinal fluid (CSF) of 23 relapsing-remitting MS patients under interferon-ß-1a therapy and 16 control subjects using ELISA. MS patients were categorized as having active or stable disease. CXCL10 was significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CXCL10; CCL2; Inflammation; Multiple sclerosis; Leukocyte recruitment.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400003

Angiotensin-(1-7) relieved renal injury induced by chronic intermittent hypoxia in rats by reducing inflammation, oxidative stress and fibrosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Lu,W.; Kang,J.; Hu,K.; Tang,S.; Zhou,X.; Yu,S.; Xu,L.
We aimed to study the renal injury and hypertension induced by chronic intermittent hypoxia (CIH) and the protective effects mediated by angiotensin 1-7 [Ang(1-7)]. We randomly assigned 32 male Sprague-Dawley rats (body weight 180-200 g) to normoxia control, CIH, Ang(1-7)-treated normoxia, and Ang(1-7)-treated CIH groups. Systolic blood pressure (SBP) was monitored at the start and end of each week. Renal sympathetic nerve activity (RSNA) was recorded. CTGF and TGF-β were detected by immunohistochemistry and western blotting. Tissue parameters of oxidative stress were also determined. In addition, renal levels of interleukin-6, tumor necrosis factor-α, nitrotyrosine, and hypoxia-inducible factor-1α were determined by immunohistochemistry, immunoblotting,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ang(1-7); Renal injury; Obstructive sleep apnea; Inflammation; Oxidative stress; Fibrosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100604

In vivo and in vitro anti-inflammatory and anti-nociceptive activities of lovastatin in rodents
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Gonçalves,D.O.; Calou,I.B.F.; Siqueira,R.P.; Lopes,A.A.; Leal,L.K.A.; Brito,G.A.C.; Tomé,A.R.; Viana,G.S.B.
Statins are among the most prescribed drugs in recent clinical practice. They are also known for their pleiotropic actions, which are independent of their lipid-lowering properties. The effect of lovastatin was investigated against carrageenan-induced paw edema in male Wistar rats (200-250 g) and on leukocyte migration, as measured by carrageenan-induced peritonitis in male Swiss mice (20-25 g), which are models of acute inflammation. Lovastatin (administered 1 h prior to carrageenan), at oral doses of 2, 5, and 10 mg/kg, markedly attenuated paw edema formation in rats at the 4th hour after carrageenan injection (25, 43, and 37% inhibition, respectively). Inhibitions of 20, 45 and 80% were observed in the leukocyte migration, as evaluated by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Statin; Inflammation; Anti-nociception; Myeloperoxidase; Cytokines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200011

Inflammatory response in a rat model of gastroschisis is associated with an increase of NF-kappaB
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Sbragia,L.; Schmidt,A.F.; Moraes,S.; Bittencourt,D.G.; Gonçalves,F.L.L.; Pereira,L.A.V.D.; Velloso,L.A.
Babies with gastroschisis have high morbidity, which is associated with inflammatory bowel injury caused by exposure to amniotic fluid. The objective of this study was to identify components of the inflammatory response in the intestine and liver in an experimental model of gastroschisis in rats. The model was surgically created at 18.5 days of gestation. The fetuses were exposed through a hysterotomy and an incision at the right of the umbilicus was made, exposing the fetal bowel. Then, the fetus was placed back into the uterus until term. The bowel in this model had macro- and microscopic characteristics similar to those observed in gastroschisis. The study was conducted on three groups of 20 fetuses each: gastroschisis, control, and sham fetuses. Fetal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastroschisis; Inflammation; Inflammatory mediators; Nuclear factor kappaB.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200006

Inflammation and oxidative stress in heart failure: effects of exercise intensity and duration
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ribeiro-Samora,G.A.; Rabelo,L.A.; Ferreira,A.C.C.; Favero,M.; Guedes,G.S.; Pereira,L.S.M.; Parreira,V.F.; Britto,R.R.
Although acute exercise is apparently pro-inflammatory and increases oxidative stress, it can promote the necessary stress stimulus to train chronic adaptations in patients with chronic heart failure (CHF). This study aimed to compare the effects of exercise intensity and duration on the inflammatory markers soluble tumor necrosis factor receptor (sTNFR1) and interleukin-6 (IL-6), and on oxidative stress [malondialdehyde (MDA) and antioxidant enzymes: catalase (CAT) and superoxide dismutase (SOD)] in individuals with CHF. Eighteen patients performed three exercise sessions: 30 min of moderate-intensity (M30) exercise, 30 min of low-intensity (L30) exercise, and 45 min of low-intensity (L45) exercise. Blood analysis was performed before exercise (baseline),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heart failure; Inflammation; Oxidative stress; Exercise intensity; Exercise duration.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900608

Short-term effects of stored homologous red blood cell transfusion on cardiorespiratory function and inflammation: an experimental study in a hypovolemia model
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Biagini,S.; Dale,C.S.; Real,J.M.; Moreira,E.S.; Carvalho,C.R.R.; Schettino,G.P.P.; Wendel,S.; Azevedo,L.C.P.
The pathophysiological mechanisms associated with the effects of red blood cell (RBC) transfusion on cardiopulmonary function and inflammation are unclear. We developed an experimental model of homologous 14-days stored RBC transfusion in hypovolemic swine to evaluate the short-term effects of transfusion on cardiopulmonary system and inflammation. Sixteen healthy male anesthetized swine (68±3.3 kg) were submitted to controlled hemorrhage (25% of blood volume). Two units of non-filtered RBC from each animal were stored under blood bank conditions for 14 days. After 30 min of hypovolemia, the control group (n=8) received an infusion of lactated Ringer's solution (three times the removed volume). The transfusion group (n=8) received two units of homologous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swine; Transfusion; Inflammation; Red blood cells; Hypovolemia; Hemorrhage.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100604

Glutamic acid decarboxylase antibodies are indicators of the course, but not of the onset, of diabetes in middle-aged adults: the Atherosclerosis Risk in Communities Study
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Vigo,A.; Duncan,B.B.; Schmidt,M.I.; Couper,D.; Heiss,G.; Pankow,J.S.; Ballantyne,C.M.
To efficiently examine the association of glutamic acid decarboxylase antibody (GADA) positivity with the onset and progression of diabetes in middle-aged adults, we performed a case-cohort study representing the ~9-year experience of 10,275 Atherosclerosis Risk in Communities Study participants, initially aged 45-64 years. Antibodies to glutamic acid decarboxylase (GAD65) were measured by radioimmunoassay in 580 incident diabetes cases and 544 non-cases. The overall weighted prevalence of GADA positivity (³1 U/mL) was 7.3%. Baseline risk factors, with the exception of smoking and interleukin-6 (P £ 0.02), were generally similar between GADA-positive and -negative individuals. GADA positivity did not predict incident diabetes in multiply adjusted (HR =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Glutamic acid decarboxylase; Auto-immune diseases; Inflammation; Risk factors.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700007

Long-chain n-3 fatty acids and inflammation: potential application in surgical and trauma patients
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Calder,P.C.
Lipids used in nutritional support of surgical or critically ill patients have been based on soybean oil, which is rich in the n-6 fatty acid linoleic acid (18:2n-6). Linoleic acid is the precursor of arachidonic acid (20:4n-6). In turn, arachidonic acid in cell membrane phospholipids is the substrate for the synthesis of a range of biologically active compounds (eicosanoids) including prostaglandins, thromboxanes, and leukotrienes. These compounds can act as mediators in their own right and can also act as regulators of other processes, such as platelet aggregation, blood clotting, smooth muscle contraction, leukocyte chemotaxis, inflammatory cytokine production, and immune function. There is a view that an excess of n-6 fatty acids should be avoided...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyunsaturated fatty acid; Fish oil; Inflammation; Eicosanoid; Cytokine; Critical illness.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400004

Mesenteric lymph reperfusion exacerbates spleen injury caused by superior mesenteric artery occlusion shock
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Li,L.L.; Zhang,C.H.; Liu,J.C.; Yang,L.N.; Niu,C.Y.; Zhao,Z.G.
The intestinal lymph pathway plays an important role in the pathogenesis of organ injury following superior mesenteric artery occlusion (SMAO) shock. We hypothesized that mesenteric lymph reperfusion (MLR) is a major cause of spleen injury after SMAO shock. To test this hypothesis, SMAO shock was induced in Wistar rats by clamping the superior mesenteric artery (SMA) for 1 h, followed by reperfusion for 2 h. Similarly, MLR was performed by clamping the mesenteric lymph duct (MLD) for 1 h, followed by reperfusion for 2 h. In the MLR+SMAO group rats, both the SMA and MLD were clamped and then released for reperfusion for 2 h. SMAO shock alone elicited: 1) splenic structure injury, 2) increased levels of malondialdehyde, nitric oxide (NO), intercellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenteric lymph reperfusion; Superior mesenteric artery; Shock; Spleen injury; Endotoxin; Inflammation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500376

Superoxide release and cellular gluthatione peroxidase activity in leukocytes from children with persistent asthma
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Marçal,L.E.; Rehder,J.; Newburger,P.E.; Condino-Neto,A.
Asthma is an inflammatory condition characterized by the involvement of several mediators, including reactive oxygen species. The aim of the present study was to investigate the superoxide release and cellular glutathione peroxidase (cGPx) activity in peripheral blood granulocytes and monocytes from children and adolescents with atopic asthma. Forty-four patients were selected and classified as having intermittent or persistent asthma (mild, moderate or severe). The spontaneous or phorbol myristate acetate (PMA, 30 nM)-induced superoxide release by granulocytes and monocytes was determined at 0, 5, 15, and 25 min. cGPx activity was assayed spectrophotometrically. The spontaneous superoxide release by granulocytes from patients with mild (N = 15), moderate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Children; Asthma; Respiratory burst; Granulocytes; Monocytes; Inflammation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100003

Immunoregulatory and anti-inflammatory effects of n-3 polyunsaturated fatty acids
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Calder,P.C.
1. Fish oils are rich in the long-chain n-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids. Linseed oil and green plant tissues are rich in the precursor fatty acid, &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;-linolenic acid (18:3n-3). Most vegetable oils are rich in the n-6 PUFA linoleic acid (18:2n-6), the precursor of arachidonic acid (20:4n-6). 2. Arachidonic acid-derived eicosanoids such as prostaglandin E2 are pro-inflammatory and regulate the functions of cells of the immune system. Consumption of fish oils leads to replacement of arachidonic acid in cell membranes by eicosapentaenoic acid. This changes the amount and alters the balance of eicosanoids produced. 3. Consumption of fish oils diminishes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphocyte; Macrophage; Immune system; Inflammation; Fish oil; Polyunsaturated fatty acid; Cytokine; Eicosanoid.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400002

Anti-inflammatory and antinociceptive effects of phonophoresis in animal models: a randomized experimental study
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cardoso,L.C.P.; Pinto,N.B.; Nobre,M.E.P.; Silva,M.R.; Pires,G.M.; Lopes,M.J.P.; Viana,G.S.B.; Rodrigues,L.M.R.
The aim of this study was to evaluate the therapeutic effects of ultrasound (US)-mediated phonophoresis alone or in association with diclofenac diethylammonium (DCF) administered topically in animal models of inflammation. A pre-clinical, prospective, and randomized experimental study of quantitative and qualitative nature was carried out. Phonophoresis was performed using a therapeutic ultrasound apparatus in two distinct models of acute inflammation. Edema was induced by an intraplantar injection of carrageenan and measured by plethysmography. The Hargreaves test was used to evaluate the antinociceptive activity and investigate the action of phonophoresis on tumor necrosis factor (TNF)-α production. A histological analysis with hematoxylin-eosin was used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phonophoresis; Ultrasonic therapy; Inflammation; Nociception; Physiotherapy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200603

Ca2+/Calmodulin-dependent kinase II delta B is essential for cardiomyocyte hypertrophy and complement gene expression after LPS and HSP60 stimulation in vitro
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cruz Junho,C.V.; Trentin-Sonoda,M.; Alvim,J.M.; Gaisler-Silva,F.; Carneiro-Ramos,M.S.
Inflammation plays an important role in the development of cardiovascular diseases (CVDs), suggesting that the immune system is a target of therapeutic interventions used for treating CVDs. This study evaluated mechanisms underlying inflammatory response and cardiomyocyte hypertrophy associated with bacterial lipopolysaccharide (LPS)- or heat shock protein 60 (HSP60)-induced Toll-like receptor (TLR) stimulation and the effect of a small interfering RNA (siRNA) against Ca2+/calmodulin-dependent kinase II delta B (CaMKIIδB) on these outcomes. Our results showed that treatment with HSP60 or LPS (TLR agonists) induced cardiomyocyte hypertrophy and complement system C3 and factor B gene expression. In vitro silencing of CaMKIIδB prevented complement gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiomyocytes; Hypertrophy; Inflammation; Complement; TLR 2/4; CaMKII.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700609

Attenuation of renal ischemic reperfusion injury by salvianolic acid B via suppressing oxidative stress and inflammation through PI3K/Akt signaling pathway
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ma,Z.G.; Xia,H.Q.; Cui,S.L.; Yu,J.
Salvianolic acid B (SAB) is one the major phytocomponents of Radix Salvia miltiorrhiza and exhibit numerous health promoting properties. The objective of the current study was to examine whether SAB exerts a renoprotective effect by attenuating oxidative stress and inflammatory response through activating phosphatidylinositol 3-kinase/serine-threonine kinase B (PI3K/Akt) signaling pathway in a renal ischemic reperfusion rat model. Forty Sprague-Dawley male rats (250–300 g) were obtained and split into four groups with ten rats in each group. The right kidney of all rats was removed (nephrectomy). The rats of the Control group received only saline (occlusion) and served as a sham control group, whereas rats subjected to ischemic reperfusion (IR) insult by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal ischemic reperfusion; Salvianolic acid B; Oxidative stress; Inflammation; PI3K/Akt pathway.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600607

Reduction of blood nitric oxide levels is associated with clinical improvement of the chronic pelvic pain related to endometriosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Rocha,M.G.; Gomes,V.A.; Tanus-Santos,J.E.; Rosa-e-Silva,J.C.; Candido-dos-Reis,F.J.; Nogueira,A.A.; Poli-Neto,O.B.
The objective of this prospective study was to determine the plasma levels of nitric oxide (NO) in women with chronic pelvic pain secondary to endometriosis (n=24) and abdominal myofascial pain syndrome (n=16). NO levels were measured in plasma collected before and 1 month after treatment. Pretreatment NO levels (μM) were lower in healthy volunteers (47.0±12.7) than in women with myofascial pain (64.2±5.0, P=0.01) or endometriosis (99.5±12.9, P&lt;0.0001). After treatment, plasma NO levels were reduced only in the endometriosis group (99.5±12.9 vs 61.6±5.9, P=0.002). A correlation between reduction of pain intensity and reduction of NO level was observed in the endometriosis group [correlation = 0.67 (95%CI = 0.35 to 0.85), P&lt;0.0001]. Reduction of NO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic pelvic pain; Endometriosis; Abdominal myofascial pain syndrome; Nitric oxide; Inflammation; Central sensitization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400363

Elevated levels of plasma osteoprotegerin are associated with all-cause mortality risk and atherosclerosis in patients with stages 3 to 5 chronic kidney disease
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Nascimento,M.M.; Hayashi,S.Y.; Riella,M.C.; Lindholm,B.
Osteoprotegerin (OPG) regulates bone mass by inhibiting osteoclast differentiation and activation, and plays a role in vascular calcification. We evaluated the relationship between osteoprotegerin levels and inflammatory markers, atherosclerosis, and mortality in patients with stages 3-5 chronic kidney disease. A total of 145 subjects (median age 61 years, 61% men; 36 patients on hemodialysis, 55 patients on peritoneal dialysis, and 54 patients with stages 3-5 chronic kidney disease) were studied. Clinical characteristics, markers of mineral metabolism (including fibroblast growth factor-23 [FGF-23]) and inflammation (high-sensitivity C-reactive protein [hsCRP] and interleukin-6 [IL-6]), and the intima-media thickness (IMT) in the common carotid arteries...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atherosclerosis; Chronic kidney disease; Inflammation; Mortality; Osteoprotegerin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100995

Thalidomide protects mice against LPS-induced shock
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Moreira,A.L.; Wang,J.; Sarno,E.N.; Kaplan,G.
Thalidomide has been shown to selectively inhibit TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; production in vitro by lipopolysaccharide (LPS)-stimulated monocytes. TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; has been shown to play a pivotal role in the pathophysiology of endotoxic shock. Using a mouse model of LPS-induced shock, we investigated the effects of thalidomide on the production of TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt; and other cytokines and on animal survival. After injection of 100-350 µg LPS into mice, cytokines including TNF-&lt;!-- $MVD$:face("Symbol") --&gt;&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;, IL-6, IL-10, IL-1ß, GM-CSF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endotoxic shock; Thalidomide; Cytokines; Inflammation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000010

Interleukin-5 mediates peritoneal eosinophilia induced by the F1 cell wall fraction of Histoplasma capsulatum
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Sá-Nunes,A.; Medeiros,A.I.; Faccioli,L.H.
An alkali-insoluble fraction 1 (F1), which contains mainly ß-glucan isolated from the cell wall of Histoplasma capsulatum, induces eosinophil recruitment into the peritoneal cavity of mice. The present study was carried out to determine the participation of interleukin-5 (IL-5) in this process. Inbred C57BL/6 male mice weighing 15-20 g were treated ip with 100 µg of anti-IL-5 monoclonal antibody (TRFK-5, N = 7) or an isotype-matched antibody (N = 7), followed by 300 µg F1 in 1 ml PBS ip 24 h later. Controls (N = 5) received only 1 ml PBS. Two days later, cells from the peritoneal cavity were harvested by injection of 3 ml PBS and total cell counts were determined using diluting fluid in a Neubauer chamber. Differential counts were performed using...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Eosinophils; Histoplasma capsulatum; Inflammation; Interleukin-5; Anti-IL-5 monoclonal antibody.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300009

High frequency of the CCR5delta32 variant among individuals from an admixed Brazilian population with sickle cell anemia
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Chies,J.A.B.; Hutz,M.H.
Homozygous sickle cell disease (SCD) has a wide spectrum of clinical manifestations. In Brazil, the main cause of death of individuals with SCD is recurrent infection. The CCR5delta32 allele, which confers relative resistance to macrophage-tropic HIV virus infection, probably has reached its frequency and world distribution due to other pathogens that target macrophage in European populations. In the present investigation a relatively higher prevalence (5.1%) of the CCR5delta32 allele was identified, by PCR amplification using specific primers, in 79 SCD patients when compared to healthy controls (1.3%) with the same ethnic background (Afro-Brazilians). Based on a hypothesis that considers SCD as a chronic inflammatory condition, and since the CCR5...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CCR5delta32; Sickle cell anemia; Inflammation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100010

Effect of endogenous hydrogen sulfide inhibition on structural and functional renal disturbances induced by gentamicin
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Francescato,H.D.C.; Chierice,J.R.A.; Marin,E.C.S.; Cunha,F.Q.; Costa,R.S.; Silva,C.G.A.; Coimbra,T.M.
Animal models of gentamicin nephrotoxicity present acute tubular necrosis associated with inflammation, which can contribute to intensify the renal damage. Hydrogen sulfide (H2S) is a signaling molecule involved in inflammation. We evaluated the effect of DL-propargylglycine (PAG), an inhibitor of endogenous H2S formation, on the renal damage induced by gentamicin. Male Wistar rats (N = 8) were injected with 40 mg/kg gentamicin (im) twice a day for 9 days, some of them also received PAG (N = 8, 10 mg·kg-1·day-1, ip). Control rats (N = 6) were treated with saline or PAG only (N = 4). Twenty-four-hour urine samples were collected one day after the end of these treatments, blood samples were collected, the animals were sacrificed, and the kidneys were removed...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Inflammation; Gentamicin nephrotoxicity; DL-propargylglycine; Hydrogen sulfide; Acute tubular necrosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300010

Plasticity of neutrophils reveals modulatory capacity
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Perobelli,S.M.; Galvani,R.G.; Gonçalves-Silva,T.; Xavier,C.R.; Nóbrega,A.; Bonomo,A.
Neutrophils are widely known as proinflammatory cells associated with tissue damage and for their early arrival at sites of infection, where they exert their phagocytic activity, release their granule contents, and subsequently die. However, this view has been challenged by emerging evidence that neutrophils have other activities and are not so short-lived. Following activation, neutrophil effector functions include production and release of granule contents, reactive oxygen species (ROS), and neutrophil extracellular traps (NETs). Neutrophils have also been shown to produce a wide range of cytokines that have pro- or anti-inflammatory activity, adding a modulatory role for this cell, previously known as a suicide effector. The presence of cytokines almost...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autoimmunity; Cancer; Infection; Inflammation; Neutrophil; Suppression.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800665

Comparison of etoricoxib and indomethacin for the treatment of experimental periodontitis in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Azoubel,M.C.F.; Menezes,A.M.A.; Bezerra,D.; Oriá,R.B.; Ribeiro,R.A.; Brito,G.A.C.
We investigated the effect of etoricoxib, a selective cyclooxygenase-2 inhibitor, and indomethacin, a non-selective cyclooxygenase inhibitor, on experimental periodontitis, and compared their gastrointestinal side effects. A ligature was placed around the second upper left molars of female Wistar rats (160 to 200 g). Animals (6 per group) were treated daily with oral doses of 3 or 9 mg/kg etoricoxib, 5 mg/kg indomethacin, or 0.2 mL saline, starting 5 days after the induction of periodontitis, when bone resorption was detected, until the sacrifice on the 11th day. The weight and survival rate were monitored. Alveolar bone loss (ABL) was measured as the sum of distances between the cusp tips and the alveolar bone. The gastric mucosa was examined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alveolar bone loss; Inflammation; Periodontitis; Cyclooxygenase inhibitors; Etoricoxib; Indomethacin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100015

Remodeling in the ischemic heart: the stepwise progression for heart
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Stefanon,I.; dos Santos,L.; Baldo,M.P.
Abstract Coronary artery disease is the leading cause of death in the developed world and in developing countries. Acute mortality from acute myocardial infarction (MI) has decreased in the last decades. However, the incidence of heart failure (HF) in patients with healed infarcted areas is increasing. Therefore, HF prevention is a major challenge to the health system in order to reduce healthcare costs and to provide a better quality of life. Animal models of ischemia and infarction have been essential in providing precise information regarding cardiac remodeling. Several of these changes are maladaptive, and they progressively lead to ventricular dilatation and predispose to the development of arrhythmias, HF and death. These events depend on cell death...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary occlusion; Inflammation; Collagen remodeling; Mechanical dysfunction; Heart failure; Rats.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900010

Tangeretin has anti-asthmatic effects via regulating PI3K and Notch signaling and modulating Th1/Th2/Th17 cytokine balance in neonatal asthmatic mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Liu,L.-L.; Li,F.-H.; Zhang,Y.; Zhang,X.-F.; Yang,J.
Asthma is a chronic allergic disease characterized by airway inflammation, airway hyper-responsiveness (AHR), and mucus hypersecretion. T-lymphocytes are involved in the pathogenesis of asthma, mediating airway inflammatory reactions by secreting cytokines. The phosphoinositide 3-kinase (PI3K) and Notch signaling pathways are associated with T cell signaling, proliferation, and differentiation, and are important in the progression of asthma. Thus, compounds that can modulate T cell proliferation and function may be of clinical value. Here, we assessed the effects of tangeretin, a plant-derived flavonoid, in experimental asthma. BALB/c mice at postnatal day (P) 12 were challenged with ovalbumin (OVA). Separate groups of mice (n=18/group) were administered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asthma; Inflammation; Notch signaling; PI3K; Tangeretin; T-helper cells.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800612

Captopril alleviates hypertension-induced renal damage, inflammation, and NF-κB activation
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Gan,Zhongyuan; Huang,Dan; Jiang,Jiaye; Li,Yuan; Li,Hanqing; Ke,Yan.
Hypertensive renal damage generally occurs during the middle and late stages of hypertension, which is typically characterized by proteinuria and renal inflammation. Captopril, an angiotensin-converting enzyme (ACE) inhibitor, has been widely used for therapy of arterial hypertension and cardiovascular diseases. However, the protective effects of captopril on hypertension-induced organ damage remain elusive. The present study was designed to explore the renoprotective action of captopril in spontaneously hypertensive rats (SHR). The 6-week-old male SHR and age-matched Wistar-Kyoto rats were randomized into long-term captopril-treated (34 mg/kg) and vehicle-treated groups. The results showed that in SHR there was obvious renal injury characterized by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Captopril; Spontaneously hypertensive rats; Renal injury; Inflammation; Nuclear factor-κB.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100602

The superoxide anion donor, potassium superoxide, induces pain and inflammation in mice through production of reactive oxygen species and cyclooxygenase-2
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Maioli,N.A.; Zarpelon,A.C.; Mizokami,S.S.; Calixto-Campos,C.; Guazelli,C.F.S.; Hohmann,M.S.N.; Pinho-Ribeiro,F.A.; Carvalho,T.T.; Manchope,M.F.; Ferraz,C.R.; Casagrande,R.; Verri Jr,W.A.
It is currently accepted that superoxide anion (O2•−) is an important mediator in pain and inflammation. The role of superoxide anion in pain and inflammation has been mainly determined indirectly by modulating its production and inactivation. Direct evidence using potassium superoxide (KO2), a superoxide anion donor, demonstrated that it induced thermal hyperalgesia, as assessed by the Hargreaves method. However, it remains to be determined whether KO2 is capable of inducing other inflammatory and nociceptive responses attributed to superoxide anion. Therefore, in the present study, we investigated the nociceptive and inflammatory effects of KO2. The KO2-induced inflammatory responses evaluated in mice were: mechanical hyperalgesia (electronic version of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superoxide anion; Potassium superoxide; Pain; Inflammation; Oxidative stress.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400321

Promoting inflammatory lymphangiogenesis by vascular endothelial growth factor-C (VEGF-C) aggravated intestinal inflammation in mice with experimental acute colitis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Wang,X.L.; Zhao,J.; Qin,L.; Qiao,M.
Angiogenesis and lymphangiogenesis are thought to play a role in the pathogenesis of inflammatory bowel diseases (IBD). However, it is not understood if inflammatory lymphangiogenesis is a pathological consequence or a productive attempt to resolve the inflammation. This study investigated the effect of lymphangiogenesis on intestinal inflammation by overexpressing a lymphangiogenesis factor, vascular endothelial growth factor-C (VEGF-C), in a mouse model of acute colitis. Forty eight-week-old female C57BL/6 mice were treated with recombinant adenovirus overexpressing VEGF-C or with recombinant VEGF-C156S protein. Acute colitis was then established by exposing the mice to 5% dextran sodium sulfate (DSS) for 7 days. Mice were evaluated for disease activity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Lymphangiogenesis; Microvessel density; Acute colitis; VEGF-C.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500602

Immunological and biochemical parameters of patients with metabolic syndrome and the participation of oxidative and nitroactive stress
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Simão,A.N.C.; Lozovoy,M.A.B.; Simão,T.N.C.; Venturini,D.; Barbosa,D.S.; Dichi,J.B.; Matsuo,T.; Cecchini,R.; Dichi,I.
Metabolic syndrome (MS) is a multifactorial disease involving inflammatory activity and endothelial dysfunction. The aim of the present study was to evaluate the relationship between the changes in lipoperoxidation, in immunological and biochemical parameters and nitric oxide metabolite (NOx) levels in MS patients. Fifty patients with MS (4 males/46 females) and 50 controls (3 males/47 females) were studied. Compared to control (Mann-Whitney test), MS patients presented higher serum levels (P &lt; 0.05) of fibrinogen: 314 (185-489) vs 262 (188-314) mg/dL, C-reactive protein (CRP): 7.80 (1.10-46.50) vs 0.70 (0.16-5.20) mg/dL, interleukin-6: 3.96 (3.04-28.18) vs 3.33 (2.55-9.63) pg/mL, uric acid: 5.45 (3.15-9.65) vs 3.81 (2.70-5.90) mg/dL, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic syndrome; Uric acid; Inflammation; Adiponectin; Nitric oxide; Lipoperoxidation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700015

Oxidative stress and skeletal muscle dysfunction are present in healthy smokers
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Neves,C.D.C.; Lacerda,A.C.R.; Lage,V.K.S.; Lima,L.P.; Tossige-Gomes,R.; Fonseca,S.F.; Rocha-Vieira,E.; Teixeira,M.M.; Mendonça,V.A.
Chronic exposure to cigarette smoke seems to be related to an increase of pro-inflammatory cytokines, oxidative stress and changes in muscular and physical performances of healthy smokers. However, these parameters have not yet been evaluated simultaneously in previous studies. The participants of this study were healthy males divided into two groups: smokers (n=20) and non-smokers (n=20). Inflammation was evaluated by measuring plasma levels of the cytokines IL-10, IL-6 e TNF-α, and of the soluble receptors sTNFR1 and sTNFR2. Oxidative stress was evaluated by determination of thiobarbituric acid reactive substances (TBARS) plasma levels, total antioxidant capacity of plasma and erythrocytes activity of the antioxidant enzymes superoxide dismutase (SOD)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Muscle endurance; Redox balance; Smoking; Tobacco cigarette.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100702

Prostanoids modulate inflammation and alloimmune responses during graft rejection
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Rocha,P.N.; Carvalho,E.M.
Acute rejection of a transplanted organ is characterized by intense inflammation within the graft. Yet, for many years transplant researchers have overlooked the role of classic mediators of inflammation such as prostaglandins and thromboxane (prostanoids) in alloimmune responses. It has been demonstrated that local production of prostanoids within the allograft is increased during an episode of acute rejection and that these molecules are able to interfere with graft function by modulating vascular tone, capillary permeability, and platelet aggregation. Experimental data also suggest that prostanoids may participate in alloimmune responses by directly modulating T lymphocyte and antigen-presenting cell function. In the present paper, we provide a brief...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thromboxane; Prostaglandins; Transplantation; Inflammation; Rejection; Corticosteroids.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200004

Th-1 and Th-2 cytokine production in infants with virus-associated wheezing
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pitrez,P.M.C.; Machado,D.C.; Jones,M.H.; Andrade,F.; Camozzato,C.; Stein,R.T.
Wheezing associated with respiratory viral infections in infancy is very common and results in high morbidity worldwide. The Th1/Th2 pattern of immune response in these patients remains unclear and previous studies have shown controversial results. The aim of the present study was to compare the type of Th1/Th2 cytokine response between infants with acute bronchiolitis, recurrent wheezing and upper respiratory infections from a developing country. Infants younger than 2 years of age admitted to Hospital São Lucas, Porto Alegre, RS, Brazil, between May and November 2001, with an acute episode of wheezing associated with viral respiratory infection were selected. Subjects with upper respiratory infections from the emergency department were selected for the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Acute bronchiolitis; Inflammation; Interferon-gamma; Interleukin-4; Cytokines.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100008

Detection of Mycoplasma pneumoniae and Chlamydia pneumoniae in ruptured atherosclerotic plaques
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Higuchi,M.L.; Sambiase,N.; Palomino,S.; Gutierrez,P.; Demarchi,L.M.; Aiello,V.D.; Ramires,J.A.F.
This paper reports what is apparently the first observation of Mycoplasma pneumoniae in association with Chlamydia pneumoniae in thrombosed ruptured atheromas. We performed electron microscopy and in situ hybridization in specimens from three patients who died of acute myocardial infarction. These patients had typical symptoms of acute ischemic syndrome. Mycoplasmas were present mainly in the lipid core of the ruptured thrombosed plaque. Vulnerable atheromas are rich in cholesterol and may favor the growth of mycoplasmas, the only microorganisms that require cholesterol for survival. We suggest that the association of Mycoplasma pneumoniae and Chlamydia pneumoniae may increase the virulence of these microorganisms, favoring proliferation, plaque...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mycoplasma pneumoniae; Chlamydia pneumoniae; Atherosclerosis; Vulnerable plaque; Inflammation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900005

Toxicity of a cyanobacterial extract containing microcystins to mouse lungs
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Picanço,M.R.; Soares,R.M.; Cagido,V.R.; Azevedo,S.M.F.O.; Rocco,P.R.M.; Zin,W.A.
Toxic cyanobacteria in drinking water supplies can cause serious public health problems. In the present study we analyzed the time course of changes in lung histology in young and adult male Swiss mice injected intraperitoneally (ip) with a cyanobacterial extract containing the hepatotoxic microcystins. Microcystins are cyclical heptapeptides quantified by ELISA method. Ninety mice were divided into two groups. Group C received an injection of saline (300 µl, ip) and group Ci received a sublethal dose of microcystins (48.2 µg/kg, ip). Mice of the Ci group were further divided into young (4 weeks old) and adult (12 weeks old) animals. At 2 and 8 h and at 1, 2, 3, and 4 days after the injection of the toxic cyanobacterial extract, the mice were anesthetized...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Microcystins; Lung; Inflammation; Alveolar collapse.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800013

Cytokine profile in childhood-onset systemic lupus erythematosus: a cross-sectional and longitudinal study
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Cavalcanti,A.; Santos,R.; Mesquita,Z.; Duarte,A.L.B.P.; Lucena-Silva,N.
Childhood-onset systemic lupus erythematosus (cSLE) exhibits an aggressive clinical phenotype and severe complications. This could be due to a pro-inflammatory cytokine milieu. Therefore, we determined plasma levels of Th1 (IL-2, IFN-γ, TNF), Th2 (IL-4), Th17 (IL-17A, IL-6), and Treg (IL-10) cytokines in a cohort of cSLE patients and healthy controls, and we evaluated the association between these cytokines and disease activity. We conducted a cross-sectional study with 51 cSLE patients from two pediatric rheumatology services. Ten cSLE patients participated in a longitudinal follow-up study. Blood samples were collected from the same patient during active and inactive disease. Disease activity was evaluated according to SLE Disease Activity Index 2000...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Childhood-onset systemic lupus erythematosus; Disease activity; SLEDAI-2K; Inflammation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400704

Impact of cigarette smoke and aerobic physical training on histological and molecular markers of prostate health in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Veras,A.S.C.; Baptista,D.B.; dos Santos,N.J.; Thorpe,H.H.A.; Seraphim,P.M.; Florido Neto,A.R.; Teixeira,G.R.
Recent evidence suggests that aerobic physical training may attenuate the deleterious effects of cancer risk factors, including smoking. We investigated the effects of cigarette smoke inhalation and aerobic physical training on the expression of steroid receptors and inflammatory and apoptotic proteins in the prostate. Forty male Wistar rats were distributed in four groups: control (CO), exercise (EXE), cigarette smoke exposure (CS), and cigarette smoke exposure with exercise (CS+EXE). For eight weeks, animals were repeatedly exposed to cigarette smoke for 30 min or performed aerobic physical training either with or without the cigarette smoke inhalation protocol. Following these experiments, we analyzed prostate epithelial morphology and prostatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Androgen receptor; Growth factors; Endurance exercise; BAX; BCL-2.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500601

Long-term ethanol intoxication reduces inflammatory responses in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Carvalho,E.M.; Brito,G.A.C.; Pessoa,B.B.G.P.; Ribeiro,R.A.; Capaz,F.R.
The anti-inflammatory effects of long-term ethanol intoxication were determined during ethanol treatment and withdrawal on the basis of neutrophil and eosinophil migration, hind paw edema and mast cell degranulation. Male Wistar rats (180-200 g, around 2 months of age) were exposed to increasing concentrations of ethanol vapor over a 10-day period. One group was evaluated immediately after exposure (treated group - intoxicated), and another was studied 7 h later (withdrawal group). Ethanol inhalation treatment significantly inhibited carrageenan- (62% for the intoxicated group, N = 5, and 35% for the withdrawal group, N = 6) and dextran-induced paw edema (32% for intoxicated rats and 26% for withdrawal rats, N = 5 per group). Ethanol inhalation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol; Inflammation; Carrageenan; Dextran; Air-pouch; Mast cells.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100013

Chronic intrathecal cannulation enhances nociceptive responses in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Almeida,F.R.C.; Schivo,I.R.S.; Lorenzetti,B.B.; Ferreira,S.H.
The influence of a chronically implanted spinal cannula on the nociceptive response induced by mechanical, chemical or thermal stimuli was evaluated. The hyperalgesia in response to mechanical stimulation induced by carrageenin or prostaglandin E2 (PGE2) was significantly increased in cannulated (Cn) rats, compared with naive (Nv) or sham-operated (Sh) rats. Only Cn animals presented an enhanced nociceptive response in the first phase of the formalin test when low doses were used (0.3 and 1%). The withdrawal latency to thermal stimulation of a paw inflamed by carrageenin was significantly reduced in Cn rats but not in Nv or Sh rats. In contrast to Nv and Sh rats, injection in Cn animals of a standard non-steroid anti-inflammatory drug, indomethacin, either...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperalgesia; Spinal sensitization; Intrathecal cannulation; Nociceptive response; Inflammation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800011

Role of matrix metalloproteinases in the development of airway inflammation and remodeling
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Lagente,V.; Manoury,B.; Nénan,S.; Le Quément,C.; Martin-Chouly,C.; Boichot,E.
Matrix metalloproteinases (MMPs) are a major group of proteases known to regulate extracellular matrix (ECM) turnover and so they have been suggested to be important in the process of lung disease associated with tissue remodeling. This has led to the concept that modulation of airway remodeling including excessive proteolysis damage to the tissue may be of interest for future treatment. Within the MMP family, macrophage elastase (MMP-12) is able to degrade ECM components such as elastin and is involved in tissue remodeling processes in chronic obstructive pulmonary disease including emphysema. Pulmonary fibrosis has an aggressive course and is usually fatal within an average of 3 to 6 years after the onset of symptoms. Pulmonary fibrosis is associated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrosis; Inflammation; Lung; Matrix metalloproteinase; Tissue inhibitor of matrix metalloproteinases; Chronic obstructive pulmonary disease.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000009

Ureases display biological effects independent of enzymatic activity: Is there a connection to diseases caused by urease-producing bacteria?
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Olivera-Severo,D.; Wassermann,G.E.; Carlini,C.R.
Ureases are enzymes from plants, fungi and bacteria that catalyze the hydrolysis of urea to form ammonia and carbon dioxide. While fungal and plant ureases are homo-oligomers of 90-kDa subunits, bacterial ureases are multimers of two or three subunit complexes. We showed that some isoforms of jack bean urease, canatoxin and the classical urease, bind to glycoconjugates and induce platelet aggregation. Canatoxin also promotes release of histamine from mast cells, insulin from pancreatic cells and neurotransmitters from brain synaptosomes. In vivo it induces rat paw edema and neutrophil chemotaxis. These effects are independent of ureolytic activity and require activation of eicosanoid metabolism and calcium channels. Helicobacter pylori, a Gram-negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urease; Canatoxin; Helicobacter pylori; Inflammation; Neutrophils; Eicosanoids.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700002

Antinociceptive and anti-inflammatory activities of a triterpene-rich fraction from Himatanthus drasticus
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: de-Almeida,S.C.X.; da-Silva,Â.C.F.; Sousa,N.R.T.; Amorim,I.H.F.; Leite,B.G.; Neves,K.R.T.; Costa,J.G.M.; Felipe,C.F.B.; de-Barros Viana,G.S.
Himatanthus drasticus (Mart.) Plumel belongs to the Apocynaceae family and the latex from its trunk bark (Hd) is known as “janaguba milk”. This latex is widely used in Northeast Brazil, mainly in the Cariri region, for its gastroprotective, anti-inflammatory, and antitumor properties. The objective of this study was to investigate a triterpene-rich fraction (FJNB) from H. drasticus latex on acute models of nociception and inflammation and to clarify its mechanisms of action. Wistar rats or Swiss mice were subjected to the carrageenan-induced paw edema test or the formalin test, respectively, after the acute oral treatment with FJNB. The inflamed paws from the carrageenan-induced paw edema and formalin tests were processed for histological and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pentacyclic triterpenes; Lupeol; Alpha-amyrin; Beta-amyrin; Inflammation; Himatanthus drasticus.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500612

Evaluation of the effects of Uncaria rhynchophylla alkaloid extract on LPS-induced preeclampsia symptoms and inflammation in a pregnant rat model
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Wu,Liang-Zhi; Xiao,Xiao-Min.
Excessive pro-inflammatory cytokines result in adverse pregnancy outcomes, including preeclampsia-like phenotypes, and fetal growth restriction. Anti-inflammation might be an effective therapy. The aim of this research was to investigate whether Uncaria rhynchophylla alkaloid extract (URE), a highly safe anti-inflammation constituent of the herb, can inhibit inflammation and improve clinical characteristics of preeclampsia in a lipopolysaccharide (LPS)-induced preeclampsia rat model. The rat model was established by daily administration of LPS (1 μg/kg body weight per day) from gestational day (GD) 14 to 19. Different doses of URE (35, 70, and 140 mg/kg body weight per day) were administered from GD 14 to GD 19. The effects of URE on proteinuria, maternal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preeclampsia; Inflammation; Uncaria rhynchophylla; Alkaloids.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600602

Reactive oxygen species and angiotensin II signaling in vascular cells: implications in cardiovascular disease
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Touyz,R.M.
Diseases such as hypertension, atherosclerosis, hyperlipidemia, and diabetes are associated with vascular functional and structural changes including endothelial dysfunction, altered contractility and vascular remodeling. Cellular events underlying these processes involve changes in vascular smooth muscle cell (VSMC) growth, apoptosis/anoikis, cell migration, inflammation, and fibrosis. Many factors influence cellular changes, of which angiotensin II (Ang II) appears to be amongst the most important. The physiological and pathophysiological actions of Ang II are mediated primarily via the Ang II type 1 receptor. Growing evidence indicates that Ang II induces its pleiotropic vascular effects through NADPH-driven generation of reactive oxygen species (ROS)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vascular smooth muscle cells; Remodeling; Inflammation; Signal transduction; Reactive oxygen species.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800018

Application of the differential display RT-PCR strategy for the identification of inflammation-related mouse genes
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Silva,A.M.; Pires,E.G.; Abrantes,E.F.; Ferreira,L.R.P.; Gazzinelli,R.T.; Reis,L.F.L.
The inflammatory response elicited by various stimuli such as microbial products or cytokines is determined by differences in the pattern of cellular gene expression. We have used the differential display RT-PCR (DDRT-PCR) strategy to identify mRNAs that are differentially expressed in various murine cell types stimulated with pro-inflammatory cytokines, microbial products or anti-inflammatory drugs. Mouse embryonic fibroblasts (MEFs) were treated with IFNs, TNF, or sodium salicylate. Also, peritoneal macrophages from C3H/Hej mice were stimulated with T. cruzi-derived GPI-mucin and/or IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt;. After DDRT-PCR, various cDNA fragments that were differentially represented on the sequencing gel were recovered, cloned and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DDRT-PCR; Gene expression; Inflammation; TNF; Interferon.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700008

NLRP3 inflammasome signaling as an early molecular response is negatively controlled by miR-186 in CFA-induced prosopalgia mice
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Chen,Ming-lei; Lin,Kang; Lin,Shu-kai.
The NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome is the most frequently studied in the central nervous system and has been linked to neuropathic pain. In this study, a post-translational mechanism of microRNA (miR)-186 via regulating the expression of NLRP3 in the complete Freund's adjuvant (CFA)-treated mice was investigated. The injection of CFA was used to induce trigeminal neuropathic pain in mice. miRs microarray chip assay was performed in trigeminal ganglions (TGs). CFA treatment significantly increased the mRNA expression of NLRP3, interleukin (IL)-1β, and IL-18 in TGs compared to the control group. Moreover, 26 miRs were differentially expressed in TGs from trigeminal neuropathic pain mice, and the expression of miR-186...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orofacial pain; Trigeminal ganglion; NLRP3 inflammasome; MiR-186; Inflammation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900607

Association between soluble biomarkers - microbial translocation, inflammation and cardiovascular risk in HIV- infected individuals: a systematic review
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Borato,Danielle Cristyane Kalva; Vellosa,José Carlos Rebuglio.
Microbial translocation is associated with the increased risk of cardiovascular disease in HIV-infected individuals. There is scarce information regarding the possible associations between the biomarkers of microbial translocation, inflammation and cardiovascular risk that can be evaluated in clinical laboratories using plasma or serum samples. This systematic review was conducted according to the PRISMA protocol in order to verify the most used soluble biomarkers of microbial translocation, inflammation and cardiovascular risk, as well as possible associations between them, in HIV-infected individuals. A search was performed using the Medline, Scopus and Web of Science databases to identify existing studies regarding the relationship between microbial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial translocation; Inflammation; Cardiovascular risk; HIV; Biomarkers.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100614

Clinacanthus nutans: Its potential against diabetic vascular diseases
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Azemi,Ahmad Khusairi; Mokhtar,Siti Safiah; Rasool,Aida Hanum Ghulam.
Diabetes is an independent risk factor for the development of cardiovascular disease, with approximately 80% of cardiovascular mortality and morbidity linked to vascular complications such as atherosclerosis. It has been estimated that up to one-third of patients with diabetes mellitus use some form of complementary and/or alternative medicine. One plant that has received attention from diabetic patients for its perceived antidiabetic properties is Clinacanthus nutans, a member of the Acanthaceae family that is known as snake grass. Ethnomedical applications of this herb have been identified for the treatment of certain conditions, including fever, diabetes, skin rashes, and insect bites. This review aims to assess the potential of C. nutans to be used in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C. nutans; Diabetes; Inflammation; Cardiovascular disease.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100635

Nonsteroidal anti-inflammatory drugs as potential ecto-nucleotide phosphodiesterase inhibitors
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Tasneem,Shumaila; Saleem,Muhammad; Saeed,Sheikh Arshad.
Phosphodiesterases (PDE) are group of enzymes which catalyze the hydrolysis of cAMP and cGMP. Since these cyclic phosphate moieties worked as intracellular second messengers in numerous physiological processes, their inhibition can affect normal physiology of living system. NSAIDs are among the frequently prescribed medications, because of their efficacy as analgesic, antipyretic and anti-inflammatory agents. They are known to block cyclooxygenase pathway. In limited data NSAIDs has been shown anti-tumor potential, and phosphodiesterase inhibition has assumed to be one of the mechanism. To date no further evaluation being done. Further, NSAIDs are classified as cyclooxygenase inhibitors and phosphodiesterase inhibition can imprint its side effects. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-steroidal antiinflammatory drugs; Cyclooxygenase; Phosphodiesterase; Inflammation; CAMP.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100531

Passion fruit (Passiflora edulis) leaf extract modulates the oxidative metabolism of rat peritoneal neutrophils in a model of inflammation
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Araújo,Cleiber Lucan Alves; Salles,Bruno Cesar Correa; Duarte,Stella Maris da Silveira; Rodrigues,Maria Rita; Paula,Fernanda Borges de Araújo.
This study was conducted to evaluate the effect of extracts of Passiflora edulis Sims leaves on the oxidative metabolism of rat peritoneal neutrophils using a model of acute inflammation. The extract was obtained by maceration in 70% ethanol, evaporation under reduced pressure and lyophilisation. Total phenolic content (TP) was determined by the Folin-Ciocalteu assay. The P. edulis extract, in different doses, was administered by gavage 1 h prior to inflammation induction by carrageenan (8 mg/kg, i.p.); five hours later, the neutrophils were obtained by intraperitoneal lavage. The tests performed in neutrophils were cytochrome C and chemiluminescence assay as well as myeloperoxidase (MPO), superoxide dismutase (SOD), glutathione peroxidase (GPx) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora edulis Sims; Inflammation; Neutrophil; Reactive oxygen species (ROS); Antioxidant enzymes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100562

Obesity, inflammation, and insulin resistance
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Martins,Luana Mota; Oliveira,Ana Raquel Soares; Cruz,Kyria Jayanne Clímaco; Torres-Leal,Francisco Leonardo; Marreiro,Dilina do Nascimento.
White adipose tissue (WAT) is considered an endocrine organ. When present in excess, WAT can influence metabolism via biologically active molecules. Following unregulated production of such molecules, adipose tissue dysfunction results, contributing to complications associated with obesity. Previous studies have implicated pro- and anti-inflammatory substances in the regulation of inflammatory response and in the development of insulin resistance. In obese individuals, pro-inflammatory molecules produced by adipose tissue contribute to the development of insulin resistance and increased risk of cardiovascular disease. On the other hand, the molecules with anti-inflammatory action, that have been associated with the improvement of insulin sensitivity, have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Inflammation; Adipose tissue; Adipokines; Insulin resistance.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400677

Protective effect of citrus lemon on inflammation and adipokine levels in acrylamide-induced oxidative stress in rats
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Haidari,Fatemeh; Mohammadshahi,Majid; Zarei,Mehdi; Fathi,Mojdeh.
The high exposure to acrylamide (AA) due to smoking and increased consumption of processed and fast foods in recent years, has become one of the health threatening problems. This study examined the effect of lemon juice on inflammation and adipokines in acrylamide-induced oxidative stress in rats. Forty animals were divided into five groups. Toxicity was induced by AA (35mg/kg) for two weeks in all groups except normal control group. After that, lemon juice in three doses was administrated to treatment groups for 4 weeks. Serum levels of adipokines and inflammatory parameters and both serum and liver levels of oxidative stress parameters were measured. The results showed groups were received AA had significant higher levels of malondialdehyde, tumor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus lemon; Acrylamide; Inflammation; Leptin; Adiponectin.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100606

Post-ride inflammatory markers in endurance horses
Provedor de dados: Ciência Rural
Autores: Siqueira,Renata Farinelli de; Fernandes,Wilson Roberto.
ABSTRACT: The acute phase reaction occurs in response to diseases, surgical trauma and can happen after strenuous exercise, like long distance events. This study aimed to evaluate the Serum Amyloid A (SAA), pro-inflammatory interleukins (IL-1β and TNFα) and creatine phosphokinase (CK) after free speed races like physical stress markers in endurance horses. Blood of 21 trained horses that competed in tests of 80, 120 and 160km, was taken before and after race. There was a significant increase in CK and SAA after the race in all horses, as well as the anti-inflammatory interleukin IL-10. But when results were compared between the groups, only the SAA was different, being higher in 160km horses. There was no change in pro-inflammatory interleukins IL-1β and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Endurance; Horses.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701256

Effect of acupuncture on TNF-alpha, IL-1b and IL-10 concentrations in the peritoneal exudates of carrageenan-induced peritonitis in rats
Provedor de dados: Ciência Rural
Autores: Scognamillo-Szabó,Márcia Valéria Rizzo; Bechara,Gervásio Henrique; Cunha,Fernando de Queiroz.
Acupuncture is an ancient and empirical therapeutic procedure known by its efficacy in the treatment of pain. However, the influence of acupuncture on inflammatory process is still poorly understood and additional research is needed. In this work, we investigated the mechanism of action of manual acupuncture on the inhibition of neutrophil migration to the peritoneal cavity induced by the inflammatory stimulus carrageenan in Wistar rats. Previous results from our laboratory showed that this anti-inflammatory effect is not due to endogenous corticoid release. Furthermore, the concentration of IL-&lt;FONT FACE=Symbol&gt;1b&lt;/FONT&gt;, but not of TNF-alpha or IL-10 in the carrageenan-induced exudates was reduced in the acupuncture group. Further research...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acupuncture; Inflammation; Cytokines; Neutrophils; Rats; Carrageenan.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000100016

Effect of an intravitreal dexamethasone device on ocular inflammation after phacoemulsification in dogs
Provedor de dados: Ciência Rural
Autores: Lima,Tiago Barbalho; Padua,Ivan Martinez; Kobashigawa,Karina Kamachi; Aldrovani,Marcela; Chacaltana,Flor Diana Yocoay Claros; Santo,Paloma do Espírito; Vieira,Lorena Carla; Barros Sobrinho,Alexandre Augusto Franchi; Cunha Junior,Armando Silva; Laus,José Luiz.
ABSTRACT: This study examined the efficacy of an intravitreal dexamethasone-loaded device for the control of postoperative ocular inflammation in dogs following phacoemulsification. Twenty dogs with bilateral mature senile cataracts were prepared for surgery using routine protocols. A biodegradable poly (lactic-co-glycolic acid) copolymer device was inserted through the pars plana into the vitreous chamber immediately before phacoemulsification (device group [DG], n=20). Following surgery, a conventional group (CG) received local and systemic steroids, mydriatics, and antibiotic therapy. The same treatment protocol was adopted in DG, except for steroids. All eyes were examined before surgery and at various times after phacoemulsification. Ultrasonography...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Cataract surgery; Inflammation; PLGA; Dexamethasone.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202216

Acute phase proteins in cats
Provedor de dados: Ciência Rural
Autores: Rosa,Rita Mourão; Mestrinho,Lisa Alexandra Pereira.
ABSTRACT:  Acute phase proteins (APP) are proteins synthesized and released largely by hepatocytes upon the occurrence of cell damage or invasion by microorganisms. This article reviews the use of APP in feline diseases, identifying their usefulness in the clinical setting, analyzing 55 published papers. Serum amyloid A, alpha-1 acid glycoprotein, and haptoglobin are the indicators pointed out by the authors as useful in monitoring the acute inflammatory response in cats. Although, APP measurement is still not routinely used in veterinary medicine, together with clinical signs and other blood parameters, was of clinical interest and applicability in diseases such as feline infectious peritonitis, pancreatitis, renal failure, retroviral and Calicivirus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Acute phase proteins; Cat.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000400600