Consulta: subjectFacets:"Staphylococcus aureus"
Registros recuperados: 148
Data/hora: 12/06/2024 16:12:52

Antimicrobial effects of plant defence peptides expressed by bovine endothelial cells on intracellular pathogens
Provedor de dados: 69
Autores: Loeza-Ángeles,Heber; López-Meza,Joel E; Ochoa-Zarzosa,Alejandra.
Background: The actions of plant antimicrobial peptides (PAP) against intracellular pathogens are poorly known. It has been reported that wheat puroindolines show antibacterial activity against Staphylococcus epidermidis endocyted by macrophages. In this work, we evaluated the intracellular antimicrobial activity of PAP gamma-thionin and thionin Thi2.1 produced by bovine endothelial cells against intracellular Staphylococcus aureus and Candida albicans. We used three host-pathogen models: 1) bovine mammary epithelial cells (BMEC)-S. aureus, 2) bovine endothelial cells (BEC)-S. aureus and 3) BEC-C. albicans, and evaluated the effect of conditioned media from BEC producers of PAP (gamma-thionin and thionin Thi2.1). Results: In the first model, conditioned...
Tipo: Journal article
Palavras-chave: Antimicrobial peptides; Arabidopsis thaliana thionin; Capsicum chinense gamma-thionin; Candida albicans; Intracellular pathogens; Staphylococcus aureus.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500001

Cultura, crescimento e identificação de bactérias do gênero Staphylococcus aureus em leite de cabra.
Provedor de dados: 14
Autores: CHAPAVAL, L.; AGUIAR, V. M. P.; SOUSA, A. P. B. de; MIRANDA, K. P. de; MORORÓ, A. M.; MAGALHÃES, D. C. T.
bitstream/CNPC-2010/23045/1/ct41.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Leite de cabra; Staphylococcus aureus; Bactéria; Microrganismo.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/748116

Diagnóstico de mastite subclínica caprina por meio da detecção de Staphylococcus aureus utilizando a técnica de RT-PCR.
Provedor de dados: 14
Autores: MONTEIRO, J. P.; SOUZA, V. de.
A presente publicação descreve os procedimentos necessários para a identificação e confirmação molecular de estirpes de S. aureus causadoras de mastite subclínica, provenientes de amostras de leite de cabra, por meio da técnica de RT-PCR.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: RT-PCR; PCR.; Leite de cabra; Caprino; Teste de mamite; Staphylococcus aureus; Diagnóstico; Infecção; Doença animal; Goat milk; Mastitis; Diagnostic techniques; Animal diseases.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1047787

Avaliação do teor de cloretos e da condutividade elétrica no leite de cabra como métodos auxiliares de diagnóstico na mastite subclínica caprina.
Provedor de dados: 14
Autores: SOUZA, V. de; FACCIOLI-MARTINS, P. Y.; MOURA, J. W. F.; FERNANDES, D. R.; LIMA, A. R.
Esta publicação avalia os testes de detecção do teor de cloretos e da condutividade elétrica (CE) em relação ao isolamento de Staphylococcus spp.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Diagnosis.; Doença animal; Teste de mamite; Staphylococcus aureus; Caprino; Diagnóstico; Cloreto; Condutividade elétrica; Animal diseases; Mastitis; Goat milk; Chlorides; Electrical conductivity.
Ano: 2017
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1086974

POPULATION ANALYSIS OF SUSCEPTIBILITY TO PROPOLIS IN REFERENCE STRAINS OF Staphylococcus aureus AND Escherichia coli
Provedor de dados: 79
Autores: FERNANDES Jr.,A.; LOPES,C. A. M.; SFORCIN,J. M.; FUNARI,S.R.C.
Susceptibility to several ethanolic extracts of propolis (EEP) concentrations was tested with the population analysis technique in reference strains of Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 35218). The results of these tests showed that all bacteria were killed by EEP concentrations approximately equal or higher (2.0% v/v- S. aureus; 10.0% v/v - E. coli) than the respective minimal inhibitory concentrations (MIC). Regarding the susceptibility to propolis, there was a homogeneity of data with the respective time kill curves showing a clear bactericidal effect during 6 to 9 h of exposition.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Population analysis; Susceptibility; Staphylococcus aureus; Escherichia coli.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000200005

Risk of peritonitis during peritoneal dialysis in carriers of Staphylococcus aureus and coagulase-negative staphylococci
Provedor de dados: 80
Autores: Batalha,J. E. N.; Caramori,J. C. T.; Corrente,J. E.; Montelli,A. C.; Barretti,P.; Cunha,M. L. R. S.
The presence of Staphylococcus aureus in the nasal cavities and pericatheter skin of peritoneal dialysis patients put them at high risk of developing peritonitis. However, it is not clear whether the presence of coagulase-negative staphylococci (CNS) in the nasal passages and skin of patients is related to subsequent occurrence of peritoneal infection. The aim of the present study was to verify the relationship between endogenous sources of S. aureus and CNS and occurrence of peritonitis in patients undergoing peritoneal dialysis. Thirty-two patients on peritoneal hemodialysis were observed for 18 months. Staphylococcus species present in their nasal passage, pericatheter skin and peritoneal effluent were identified and compared based on drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Coagulase-negative staphylococci; Carriers; Peritoneal dialysis; Peritonitis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400005

Septic arthritis: immunopathogenesis, experimental models and therapy
Provedor de dados: 80
Autores: Colavite,Priscila Maria; Sartori,Alexandrina.
Septic arthritis is an inflammatory disease of the joints that is started by an infection whose most common agent is Staphylococcus aureus. In this review we discuss some of the most arthritogenic bacterial factors and the contribution of innate and specific immune mechanisms to joint destruction. Special emphasis is given to the induction of experimental arthritis by S. aureus in mice. The improvement of therapy by association of antibiotics with down-modulation of immunity is also included.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Septic arthritis; Staphylococcus aureus; Mice.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200205

Antimicrobial activity of propolis and essential oils and synergism between these natural products
Provedor de dados: 80
Autores: Probst,IS; Sforcin,JM; VLM,Rall; Fernandes,AAH; Fernandes Júnior,A.
In the present study, Apis mellifera propolis and essential oils (EOs) obtained from aromatic plants were evaluated as alternative antimicrobials. We aimed to establish the antimicrobial activity of ethanolic extracts of propolis (EEP) from Apis mellifera and of EOs from Caryophyllus aromaticus, Zingiber officinale, Cinnamomum zeylanicum and Mentha piperita against 32 Staphylococcus aureus and Escherichia coli strains from human clinical specimens. The antimicrobials were diluted in agar and the minimal inhibitory concentrations (MIC) were found whereas MIC90% values were calculated. Time-kill curve assays using mixtures containing one quarter of MIC90% for EEP with all EOs as well cinnamon EO were performed. The cinnamon EO was found to be the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apis mellifera; Aromatic plants; Ethanolic extract of propolis; Staphylococcus aureus; Escherichia coli.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200006

A new sterile technique effective on capturing tramp ants for microbiological investigations
Provedor de dados: 82
Autores: Schuller,Lucia; Matté,Glavur R; Matté,Maria H.
Tramp ants are an outstanding group of organisms that have a definitive and important role in carrying pathogens. The purpose of this study was to develop a microbiological sterile technique of collecting ants from contaminated areas. The traps were composed of Petri dishes containing culture media, and were tested to verify the applicability of the system. A positive correlation between the microorganism growth and the presence of ants inside or around traps was observed. The technique described demonstrated to be useful to collect ants from different environments, helping the surveillance of pathogenic microorganisms that are of public health concern.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Public health; Pathogenic microorganism; Staphylococcus aureus; Klebsiella; Escherichia coli; Enterobacter.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2009000400023

Qualidade microbiológica e vida útil de filés defumados de tilápia-do-nilo sob refrigeração ou congelamento
Provedor de dados: 86
Autores: Franco,Maria Luiza Rodrigues de Souza; Amaral,Luiz Augusto do; Viegas,Elisabete Maria Macedo; Kronka,Sérgio do Nascimento; Gasparino,Eliane; Mikcha,Jane Martha Graton; Del Vesco,Ana Paula.
O objetivo deste trabalho foi avaliar a qualidade microbiológica e a vida útil de filés de tilápia-do-nilo, submetidos a diferentes métodos de defumação e condições de armazenamento. Foram utilizados dois processos de defumação (a frio ou a quente), em filés com ou sem pigmentação. Os produtos foram armazenados sob refrigeração ou congelados, e monitorados por 28 dias para avaliação da vida útil. Os filés congelados foram monitorados continuamente por 146 dias, apenas para a análise de ácido tiobarbitúrico (TBA). Defumação a quente e a frio reduziram a quantidade de coliformes, respectivamente em 99,78% e 97,80%. O armazenamento do produto sob refrigeração permitiu a redução de 99,73% dos coliformes, e o armazenamento sob congelamento os reduziu em 99,83%....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oreochromis niloticus; Staphylococcus aureus; Coliformes fecais; Defumação a frio; Defumação a quente.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800040

Lytic bacteriophages as a potential alternative to control Staphylococcus aureus
Provedor de dados: 86
Autores: Leite,Juliana Almeida; Pereira,Hyago Passe; Borges,Cristiano Amâncio Vieira; Alves,Bruna Rios Coelho; Ramos,Alessandra Isis Alves Pinheiro; Martins,Marta Fonseca; Arcuri,Edna Froeder.
Abstract: The objective of this work was to characterize autochthonous bacteriophages and to determine their lytic activity on Staphylococcus aureus. Six phages were isolated from dairy barn flush water through enrichment cultures with three S. aureus strains. All phages were characterized by DNA digestion by restriction enzymes and sequencing of the DNA fragment encoding endolysin. Each phage was tested against 100 S. aureus strains isolated from bovine mastitis and from dairy products using the lysis-plate method. The sequences of the endolysin gene were highly conserved, with nucleotide similarity higher than 99% among the isolated phages. Three domains involved in the recognition and lysis of the bacterial cell wall were identified. Two bacteriophages...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Biological control; Endolysin; Phage.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000103303

Antimicrobial effect of turmeric (Curcuma longa) on chicken breast meat contamination
Provedor de dados: 89
Autores: Lourenço,TC; Mendonça,EP; Nalevaiko,PC; Melo,RT; Silva,PL; Rossi,DA.
The aim of this study was to evaluate the efficacy of turmeric (Curcuma longa), also known in Brazil as saffron, on the reduction of Staphylococcus aureus and Escherichia coli counts in chicken meat. Forty breast meat samples were divided in two groups (A and B). In group A, 10³-10(4)E. coli (ATCC 25922) cells were inoculated and group B samples were inoculated with 10(4)-10(5)S. aureus (ATCC 9801) cells, after which each group was divided in three samples. The first sample was analyzed immediately after inoculation. The second sample (control group) was stored at 4 ºC for 48 hours and turmeric at 1% (w/w) was added to the third sample, which was homogenized and then stored under the same conditions as the second sample. E. coli and S. aureus were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Turmeric; Bacterial growth; Escherichia coli; Staphylococcus aureus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000200002

Bacteriological and Histopathological Evaluation of Articulations of Chickens Diagnosed with Arthritis
Provedor de dados: 89
Autores: Marcon,AV; Oliveira,GF De; Caldara,FR; Garcia,RG; Matins,RA; Marcon,A; Crone,C; Assunción,ASA.
ABSTRAT Arthritis implies inflammation of the intra-articular structures and is one of the causes of condemnation in broilers. The disease is associated with infectious agents such as Staphylococcus aureus, which may pose a potential public health risk. This work was conducted with the objective to determine the occurrence of arthritis in broiler chickens, predominantly of bacterial staphylococcal origin and if there is a difference between the degrees of injury regarding the presence of the bacterium and the histological lesion. Sixty samples of chickens diagnosed with arthritis by Federal Inspection Service from commercial slaughterhouse were collected according to their severity (of mild and severe degree) and submitted to bacteriological and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious; Inflammatory; Public health; Staphylococcus aureus.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200500

Consórcio suíno-peixe: aspectos ambientais e qualidade do pescado
Provedor de dados: 96
Autores: Pilarski,Fabiana; Tomazelli Júnior,Osmar; Casaca,Jorge de Matos; Garcia,Flávio Roberto Mello; Tomazelli,Ingrid Boesche; Santos,Ieda Rottava dos.
Amostras de músculo de carpa comum (Cyprinus carpio) e de água de três viveiros fertilizados com dejetos de suínos e de um viveiro em que os peixes foram alimentados com ração comercial para peixes foram analisadas durante seis meses. O objetivo foi observar a presença de bactérias patogênicas, como Salmonella, Staphylococcus aureus e coliformes totais e fecais, em peixes comercializados in natura no município de Chapecó, Santa Catarina. Foram aferidos quinzenalmente, na água dos quatro viveiros, oxigênio dissolvido, pH, turbidez, alcalinidade total, dureza total, amônia, nitrato e ortofosfato, bem como a quantidade de coliformes totais e fecais. A análise microbiológica músculo dos peixes demonstrou ausência de Salmonella em todas as amostras. A contagem...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyprinus carpio; Dejetos suínos; Salmonella; Staphylococcus aureus; Coliformes totais e coliformes fecais.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982004000200001

Interferência da microbiota autóctone do queijo coalho sobre Staphylococcus coagulase positiva
Provedor de dados: 124
Autores: Machado,Terezinha Feitosa; Borges,Maria de Fátima; Porto,Bruna de Castro; Sousa,Cívita Teixeira de; Oliveira,Francisco Edílson Moreno de.
Níveis elevados de contaminantes microbiológicos, comumente encontrados em alimentos de origem animal e ambientes de processamento de alimentos, são aptos de impedir o crescimento de patógenos nestes produtos. Em alguns contextos onde bactérias ácido láticas (BAL) constituem a microbiota dominante, como nos produtos lácteos, Staphylococcus coagulase positiva (SCP) colonizam, persistem e produzem intoxicação alimentar. Com o objetivo de verificar a possível interferência da microbiota encontrada no queijo Coalho sobre a presença de SCP, 64 amostras provenientes de 16 laticínios foram submetidas a análises microbiológicas para determinar os níveis de microrganismos aeróbios mesófilos (MAM), BAL e SCP. Os resultados obtidos mostraram que a microbiota...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Segurança alimentar; Staphylococcus aureus; Bactérias produtoras de ácido lático.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902011000200011

Actividad antiséptica de vinagre de Guadua angustifolia Kunth
Provedor de dados: 98
Autores: Mejía Gallón,Amanda Inés; Cadavid Torres,Elizabeth; Gallardo Cabrera,Cecilia.
Introducción: los vinagres de bambú se utilizan en los países orientales en aplicaciones farmacéuticas, con la especie Guadua angustifolia Kunth no se han encontrado investigaciones similares. Evaluar la actividad antiséptica del vinagre de guadua permitiría avanzar en estos desarrollos. Objetivo: determinar la sensibilidad de las bacterias Pseudomona aeruginosa y Staphylococcus aureus frente al vinagre de guadua, los cambios con la concentración y el pH. Métodos: la actividad antimicrobiana se midió por difusión en agar en medio Muller Hinton. Se ensayaron vinagres a concentraciones entre 100, 80, 60, 40 y 20 % a pH entre 3 y 6. Se estableció la concentración mínima inhibitoria. Resultados: el vinagre de guadua presenta actividad antiséptica a...
Tipo: Journal article
Palavras-chave: Pseudomona aeruginosa; Staphylococcus aureus; Ácido acético; Vinagre de Guadua angustifolia Kunth; Actividad antiséptica.
Ano: 2011
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962011000300004

Atividade antimicrobiana in vitro de Geraniol e Cariofileno sobre Staphylococcus aureus
Provedor de dados: 98
Autores: Melo Coutinho,Henrique Douglas; de Freitas,Maria Audilene; Figueiredo Leite Gondim,Cícera Natália; Sabino de Albuquerque,Rosimeire; de Alencar Ferreira,João Victor; Cosmo Andrade,Jacqueline.
Tipo: Journal article
Palavras-chave: Atividade antimicrobiana; Cariofileno; Geraniol; Resistência microbiana; Staphylococcus aureus.
Ano: 2015
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962015000100009

INFLUÊNCIA DO BINÔMIO TEMPO/TEMPERATURA NA ATIVIDADE BACTERICIDA DO EXTRATO AQUOSO DE Moringa oleifera LAM.
Provedor de dados: 107
Autores: Sousa, João Paulo Gomes; Rosa, Carolini Esmeriz; Assunção, Daiane Evelin dos Santos; Paiva, Ed Carlo Rosa; Barros, Jupyracyara Jandyra de Carvalho.
A estabilidade da atividade antimicrobiana do extrato aquoso da semente de Moringa oleifera Lam, em relação ao tempo/temperatura, foi testada a partir da técnica de difusão em disco nas espécies Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 e Pseudomonas aeruginosa ATCC 27853. Os extratos foram colocados em quatro temperaturas diferentes (8 ºC, 25 ºC, 37º C e 42 ºC) e monitorados no tempo zero, três dias, sete dias e dez dias e a única bactéria susceptível a eles foi a Staphylococcus aureus ATCC 25923 (halo de inibição &gt; 13 mm). O aumento no volume do extrato não levou ao aumento da efetividade no controle de Staphylococcus aureus ATCC 25923. A partir de três dias, foi observado decréscimo na atividade do extrato, no que se refere à ação...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recursos Hídricos e Ambientais estabilidade térmica; Conservação de alimentos; Staphylococcus aureus.
Ano: 2016
URL: http://www.seer.ufv.br/seer/index.php/reveng/article/view/593

Resistencia a penicilina G y oxacilina, de cepas de Staphylococcus aureus aisladas de mastitis bovina subclínica
Provedor de dados: 108
Autores: Zschöck,Michael; El-Sayed,Amr; Eissa,Nawara; Lämmler,Christoph; Castañeda-Vazquez,Hugo.
De 530 diferentes cepas de S. aureus aisladas de casos de mastitis subclínica bovina, se seleccionaron 68 cepas de S. aureus procedentes de Alemania (n = 26), Indonesia (n = 16), México (n = 16) y Brasil (n = 10), para estudiarlas en la presente investigación. Las cepas fueron analizadas fenotípica y genotípicamente para observar su resistencia a penicilina G y oxacilina. Para una identificación inicial se utilizó el sistema Api 32 Staph y la prueba de coagulasa. El resultado se confirmó por la detección molecular de los genes específicos de S. aureus 23S rRNA, nucleasa termoestable (nuc), factor aglutinante (clfA), coagulasa (coa) y la proteína A (spa) región Xr. La selección primaria de las cepas sospechosas se hizo al azar, seleccionando una cepa por...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Mastitis bovina; Alemania; Indonesia; México; Brasil; Oxacilina; Penicilina G; Resistencia.
Ano: 2011
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922011000300002

Resistência estafilocócia ao nacl em leite desnatado reconstituído adicionado de nisina
Provedor de dados: 195
Autores: Lima, T. S.; Vieira, P.; Cunha, A. L. F. S.; Cabrini, C. C.; Pinto, M.
Na atualidade, no Estado de Minas Gerais, Brasil, os queijos artesanais têm ocupado uma posição de destaque em função da sua importância social e econômica. A cada ano estão ganhando maior notoriedade, entrando em diversas microrregiões produtoras no circuito tradicional, tais como as microrregiões do Campo das Vertentes e a emancipação da Serra do Salitre da microrregião do Cerrado. Há alguns anos, esse queijo só podia ser comercializado dentro do Estado de Minas Gerais, mas atualmente sua presença fora de Minas Gerais é notória. Pelo fato de ser elaborado com leite cru, o queijo apresenta risco para os consumidores por ser potencial carreador de microrganismos patogênicos como o Staphylococcus aureus. A obrigatoriedade da utilização de leite cru implica...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Segurança de alimentos; Coliformes; Escherichia coli; Staphylococcus aureus.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/34986

Estudo da susceptibilidade de Staphylococcus aureuS isolados do leite de vacas com mastite em Sergipe
Provedor de dados: 195
Autores: Neres, Wemerson de Santana; Tuñón, Gabriel Isaias Lee; Santos, Osmário Marques; Carneiro, Maria Regina Pires.
O presente trabalho verificou a ocorrência de Staphylococcus aureus multirresistentes em amostras de leite de vacas com mastite em Sergipe. Foi realizado o diagnóstico da mastite clínica pela detecção de alterações macroscópicas no úbere e no leite. A mastite subclínica foi diagnosticada com o Califórnia Mastite Teste (CMT). Foram coletadas 40 amostras de leite e isoladas 69 estirpes de S. aureus a partir de testes convencionais. Realizou-se a triagem com a técnica de difusão em discos de Kirby Bauer com cefoxitina (30mg) para identificação fenotípica de Staphylococcus aureus resistente à meticilina (MRSA). Isolados foram testados frente a 10 princípios ativos com o teste de sensibilidade a antimicrobianos. Do total de amostras, 35 (87,5%) foram advindas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastite clínica e subclínica; Staphylococcus aureus; Multirresistência.
Ano: 2015
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/25212

Análise microbiológica do queijo minas artesanal do Serro-MG, Brasil, produzido com e sem fermento natural
Provedor de dados: 195
Autores: Cunha, A. L. F. S.; Paiva, V. N.; Cabrini, C. C.; Vieira, P.; Pinto, M. S.
Os queijos Minas artesanais possuem grande importância econômica, social, ambiental e política para o estado de Minas Gerais, sendo responsáveis pelo sustento direto e indireto de mais de 35 mil famílias. Até o presente momento não existem estudos envolvendo a utilização do fermento natural na fabricação de queijos Minas artesanais. Sabe-se apenas que o mesmo é utilizado de forma indiscriminada em algumas regiões produtoras tradicionais onde alguns produtores utilizam poucos mililitros para cada 100 litros de leite enquanto outros utilizam até um litro para os mesmos 100 litros. Além disso, segundo os produtores, as perdas de produção são atribuídas à qualidade desse fermento natural. O presente trabalho avaliou o efeito da adição de fermento natural sobre...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Segurança Alimentar; Coliformes; Escherichia coli; Staphylococcus aureus.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/34810

Low cost production and purification of polyclonal antibodies to staphylococcal enterotoxin A
Provedor de dados: 59
Autores: Oliveira,Tereza Cristina R.M. de; Hirooka,Elisa Yoko.
An immunization scheme for production of antiserum to staphylococcal enterotoxin A (SEA) is proposed. The reference method of Robbins and Bergdoll was modified to reduce the number of doses and the amount of toxin used per animal. The best immunization scheme used injections in days 0, 8, 24, 59, 62 and 67. The amount of toxin at each injection was 5, 6, 20, 50, 100 and 200&lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;g, respectively. The total amount of toxin was 381&lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;g, which corresponded to a reduction of 107&lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;g in the amount of toxin for each animal when compared to the reference method. The average antiserum titer using the Optimum Sensitivity Plate - OSP was 1:60 and using ELISA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Enterotoxins; Detection; Immunization.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200006

Cepas de Lactobacillus spp. con capacidades probióticas aisladas del tracto intestinal de terneros neonatos
Provedor de dados: 101
Autores: Sánchez,Lilian; Omura,Michele; Lucas,Adams; Pérez,Tania; Llanes,Marisleidys; Ferreira,Celia de Luce.
Se caracterizaron in vitro 17 cepas de Lactobacillus spp. aisladas del tracto intestinal de terneros neonatos, para seleccionar las mejores como candidatos probióticos y realizarles posteriormente los estudios in vivo. A todos los aislados se les realizó la prueba de la catalasa, la tinción de Gram y se estudió sus características culturales para su identificación preliminar. Se evaluó su capacidad probiótica mediante pruebas in vitro como fueron el crecimiento a diferentes temperaturas (30, 37 y 45°C), pH (2,5; 3,4; 5,4; 6,4) y capacidad de acidificación del medio de cultivo. Se preseleccionaron 10 cepas de Lactobacillus spp. que crecieron en todas las condiciones evaluadas. Se destaca el crecimiento de células viables de 6,7-10 log UFC/ml que...
Tipo: Journal article
Palavras-chave: Lactobacillus spp.; Probiótico; Salmonella sp.; Staphylococcus aureus; Escherichia coli; Listeria monocytogenes.
Ano: 2015
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000200004

PRIMER INFORME EN CUBA DE VARIACIÓN GENOTÍPICADE Staphylococcus aureus AISLADO DE LECHE BOVINA
Provedor de dados: 101
Autores: Peña,J; Uffo,Odalys.
Staphylococcus aureus es un contaminante importante y frecuente de la leche. Actualmente los estudios de tipificación genética arrojan datos importantes para el control de su diseminación; los que se basan en la variabilidad genética de la especie bacteriana. Con el objetivo de detectar polimorfismo genético se aplicaron técnicas de tipificación basados en la Reacción en Cadena de la Polimerasa (PCR) de los genes coa y RNAr en aislamientos de S. aureus, a partir de leche de tanque de varias empresas de provincia La Habana y un caso de mastitis clínica. Por primera vez se comprobó la variabilidad genética de Staphylococcus aureus aislados en Cuba y se demostró la utilidad de las técnicas empleadas a nivel de zonas geográficas relativamente distantes y...
Tipo: Journal article
Palavras-chave: Staphylococcus aureus; Tipificación; Variabilidad genética; Leche.
Ano: 2010
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2010000200010

Producción de biofilme en genotipos de Staphylococcus aureus aislados de mastitis bovina en Cuba
Provedor de dados: 101
Autores: Peña,Joan; Uffo,Odalys.
Staphylococcus aureus es uno de los patógenos más importante de la mastitis bovina. La producción de biofilme por parte de las bacterias asociadas a las infecciones intramamarias, les confiere mayor capacidad de colonizar el tejido y de protección contra el sistema inmune y contra la acción de antimicrobianos. En este estudio, se trabajó con 98 aislados cubanos de S. aureus, previamente genotipados para la región X de la proteína A, colectados de cinco provincias de Cuba y se testaron para la producción de slime y biofilme. Se observó que el 98% de los aislados tuvieron algún tipo de producción de biofilme. Se considera que el método por tinción con Violeta Cristal utilizando placas de poliestireno es el método de elección, pues el método de producción de...
Tipo: Journal article
Palavras-chave: Biofilme; Slime; Staphylococcus aureus; Mastitis bovina; Genotipo Spa.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000300007

Prevalence, somatic cell count and etiology of bovine mastitis in Cuban herds from Mayabeque province using hand and machine milking
Provedor de dados: 101
Autores: Kent Ruiz,Amado; Peña,Joan; González,Dayaimi; Ponce,Pastor.
Few studies have been developed to evaluate the differences of bovine mastitis situation between hand and machine milking under the American tropic conditions. Twenty dairy herds were studied, 11 using hand milking (HM) and 9 machine milking (MM), from «San José de las Lajas» municipality, Mayabeque province. Between May 2009 and March 2012, samples from 182 cows (113 MM and 69 HM) were obtained meaning a 35.1% of total milking cows, resulting in 708 quarters sampled (435 MM and 273 HM). Samples were subjected to bacteriological diagnosis and somatic cell count (SCC). There were significant differences for the prevalence of blind quarters, subclinical mastitis and intramammary infections between hand milking, 1.1; 29.4 and 59.4% and machine milking, 3.8;...
Tipo: Journal article
Palavras-chave: Bovine mastitis; Coagulase negative Staphylococcus; Corynebacterium bovis; Staphylococcus aureus; Streptococcus agalactiae.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000100002

Caracterización in vitro de bacterias ácido lácticas con potencial probiótico
Provedor de dados: 101
Autores: Sánchez,Lilian; Tromps,Jeannette.
Las propiedades beneficiosas de las bacterias ácido lácticas (BAL) posibilitan su uso como probióticas en animales y humanos. El objetivo del presente trabajo fue caracterizar, mediante pruebas in vitro, cepas BAL procedentes de fuente animal para considerar su posterior aplicación como probiótico en animales. Se evaluaron 72 cepas, identificadas hasta el nivel de género, de Lactobacillus spp., Leuconostoc spp., Lactococcus spp., Streptococcus spp. y Pediococcus spp. A todas las cepas se les determinó su estabilidad de crecimiento a diferentes pH, temperatura y tolerancia a altas concentraciones de cloruro de sodio. De ellas se seleccionaron 14 cepas que presentaron las mejores características como candidatos a probióticas por sus resultados a las pruebas...
Tipo: Journal article
Palavras-chave: Bacterias ácido-láctica; Probióticos; Bacillus cereus; Staphylococcus aureus; Salmonella spp.; Pseudomonas aeruginosa.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000200008

AVALIAÇÃO DA ATIVIDADE ANTIBACTERIANA E MODULADORA DE AMINOGLICOSÍDEOS DO ÓLEO ESSENCIAL DE Cymbopogon citratus (DC.) Stapf
Provedor de dados: 37
Autores: F. LUCENA,Bruno F; TINTINO,Saulo R; FIGUEREDO,Fernando G; DE M. OLIVEIRA,Cícera Datiane; DOS S. AGUIAR,José J; DO N. CARDOSO,Edmilson; A. DE AQUINO,Pedro E; ANDRADE,Jacqueline C; M. COUTINHO,Henrique D; F. MATIAS,Ednardo F.
Vários trabalhos vêm demonstrando a importância do estudo de produtos naturais como fonte alternativa para novos antimicrobianos ou que venham potencializar os já existentes. Neste contexto este trabalho teve como objetivo investigar a atividade antibacteriana e as possíveis interações entre o óleo essencial de Cymbopogon citratus combinados a aminoglicosídeos frente a linhagens padrões e multirresistentes de S. aureus, E. coli e de P. aeruginosa provenientes de isolados clínicos. Um ensaio de microdiluição foi realizado para verificar a atividade antibacteriana e as possíveis interacções entre o produto natural e os antibióticos, utilizando uma concentração sub-inibitória. Através dos resultados foi constatado a interferência sinérgica dos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cymbopogon citratus; Escherichia coli; Óleo essencial; Pseudomonas aeruginosa; Staphylococcus aureus.
Ano: 2015
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000100005

Caracterización genotípica de Staphylococcus aureus resistente a la meticilina prevalente en hospitales de Pakistán
Provedor de dados: 38
Autores: Majeed,Zahid; Arafat,Yasrab; Ajab,Zainab; Akbar Malik,Salman; Shehzad Abbasi,Waseem; Ajab,Huma.
El gen de la proteína A se usó como marcador genético para la caracterización de aislados de Staphylococcus aureus resistentes a la meticilina (SAMR). De un total de 130 aislados de Staphylococcus aureus, 90 fueron identificados como SAMR y 81 de éstos se pudieron caracterizar por tipificación spa. Todos estos aislados fueron obtenidos de cinco Hospitales Nacionales de la Comunidad. Se utilizaron dos juegos diferentes de cebadores para amplificar la región-X del gen de la proteína A en las cepas de SAMR. Un conjunto de cebadores, spa-F/spa-R ha identificado tres tipos de repeticiones diferentes, a saber, 7 repeticiones (spa 2), 8 repeticiones (spa 3) y 10 repeticiones (spa 4) y otro conjunto de cebadores, spa-1113F/spa-1514R ha identificado 4 tipos de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotipo; Staphylococcus aureus; Proteína A; Tipificación spa; Polimorfismo.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572012000200011

Distribución de genes de adhesión y regulación de biofilm en Staphylococcus aureus
Provedor de dados: 38
Autores: Cruz Martínez,Sofía Francisca; Tapia Pastrana,Gabriela; Castañón Sánchez,Carlos Alberto.
La formación de biofilms es un importante factor de virulencia que contribuye en la cronicidad de los procesos infecciosos producidos por Staphylococcus aureus. Las proteínas presentes en su superficie pueden promover la formación de biofilm. En este estudio se comparó la distribución de genes que codifican para proteínas de adhesión asociadas con la formación de biofilm en cepas resistentes (MRSA) y sensibles a meticilina (MSSA). Al analizar un total de 106 aislados obtenidos de muestras sólidas y líquidas recuperados de un hospital de México, se determinó que la formación de biofilm está asociada de manera significativa a cepas MRSA (83%). Mediante la reacción en cadena de la polimerasa (PCR), se buscó la presencia de nueve genes de adhesinas (eno, ebps,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Biofilm; Resistencia a meticilina; Gen cna; Adhesión.
Ano: 2016
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572016000400020

Portación nasal de Staphylococcus aureus en individuos de la comunidad: factores epidemiológicos
Provedor de dados: 38
Autores: Fosch,Sonia; Yones,Cristian; Trossero,Marta; Grosso,Omar; Nepote,Andrea.
La portación nasal de Staphylococcus aureus (SAU) en individuos sanos confirma su diseminación, constituye una fuente potencial de infección y se la relaciona con patologías atópicas. El objetivo del trabajo fue determinar la prevalencia de colonización nasal de SAU en individuos de la comunidad, sus respectivos patrones de resistencia antimicrobiana (PRA) y su asociación con factores epidemiológicos. Se realizó un estudio en 150 hisopados nasales. Se identificaron los aislados de SAU por métodos convencionales, y se evaluó su sensibilidad antimicrobiana por el método de difusión con discos de Kirby-Bauer y en cepas de Staphylococcus aureus resistentes a meticilina (SAMR) se confirmó la presencia del gen mec A y se investigó el gen lpv codificante de la...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Colonización nasal; Comunidad; Resistencia antimicrobiana; Patologías atópicas.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572012000100009

Antibacterial activity of Aroeira honeys produced in Minas-Gerais against bacteria of clinical importance
Provedor de dados: 3
Autores: Viana, Fabiana Ribeiro; Carmo, Luiz Simeão do; Bastos, Esther Margarida Alves Ferreira.
The biological activity of honey has been the subject of great scientific investigation. Bee products are widely used in folk medicine to treat human diseases due to their antibacterial and inhibitory potential action on different human pathogens. Ten honey samples produced by Apis mellifera in the northern of Minas Gerais state were tested against pathogenic microorganisms Staphylococcus aureus TSST (clinical isolated) and enterohaemorrhagic Escherichia coli ATCC 43895 for determination of their minimum inhibitory concentrations. The microdilution technique in broth Mueller-Hinton broth (MHB) was used in four concentrations (25, 12.5, 6.25 and 3.125%). There was a reduction of bacterial growth for the two target species at all concentrations tested. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bee products; Antibacterial activity; Staphylococcus aureus; Escherichia coli.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36766

Antibacterial activity and chemical composition of essential oil of Lippia microphylla Cham - doi: 10.4025/actascibiolsci.v33i2.5792
Provedor de dados: 3
Autores: Rodrigues, Fabiola Fernandes Galvão; Universidade Regional do Cariri; Coutinho, Henrique Douglas Melo; Universidade Regional do Cariri; Campos, Adriana Rolim; Universidade Regional do Cariri; Lima, Sidney Gonçalo; Universidade Federal do Piauí; Costa, José Galberto Martins; Universidade Regional do Cariri.
The essential oil from the fresh leaves of Lippia microphylla Cham. was obtained by hydrodistillation and characterized by gas chromatography-mass spectrometry (GC-MS). Major constituents of the oil were 1.8-cineole (18.12%), &amp;beta;-ocimene (15.20%), bicyclogermacrene (11.63%) and caryophyllene oxide (8.32%). Antimicrobial activity of the oil against Gram-positive and Gram-negative bacteria was determined by gel diffusion method. The oil showed good antibacterial activity against Shigella flexneri, Escherichia coli and Streptococcus pyogenes and very good antibacterial activity against Staphylococcus aureus.
Palavras-chave: 2.03.06.00-8 antibacterial activity; 1 8-cineole; Verbenaceae; Staphylococcus aureus Botanica Aplicada antibacterial activity; 1 8-cineole; Verbenaceae; Staphylococcus aureus.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5792

Antibiotic activity of Plectranthus ornatus Codd., a Traditional Medicinal Plant
Provedor de dados: 42
Autores: NASCIMENTO,FERNANDA R.; ALBUQUERQUE,KAMYLLA R.S.; OLIVEIRA,MARCOS R.; PIZZIOLO,VIRGINIA R.; BRASILEIRO,BEATRIZ G.; DIAZ,GASPAR; DIAZ,MARISA A.N.
ABSTRACT The dichloromethane extract of Plectranthus ornatus Codd., a tradicional medicinal plant, showed antibiotic activity with minimum inhibitory concentration (MIC) values of 0.4 mg.mL-1 and 100 percent of biofilm inhibition against Staphylococcus aureus strains isolated from animals with mastitis infections. Based on these antibacterial activities, in addition to ethnopharmacological reports from healing men and farmers in Brazil, an herbal soap was produced from this active extract and was tested both in vitro and in vivo. In vivo assays conducted on these herbal soaps led to results similar to those previously conducted with the active extract. These results indicated the great potential of this plant for use as an excipient by preparing herbal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine mastitis; Herbal soaps; Plectranthus ornatus; Staphylococcus aureus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502461

Plant Extracts Display Synergism with Different Classes of Antibiotics
Provedor de dados: 42
Autores: SILVA,DANIELLE M.; COSTA,PRISCILLA A. DA; RIBON,ANDRÉA O.B.; PURGATO,GISLAINE A.; GASPAR,DIAZ-MUÑOZ; DIAZ,MARISA A.N.
Abstract: One manner in which plant-derived compounds exert their antibiotic potential is the synergism, a positive interaction between two compounds. Studies indicate that the use of plant extracts combined with antimicrobials may promote a significant reduction of the minimum inhibitory concentrations of antibiotics for bacterial strains. This study aimed to evaluate the activity of plant extracts and antibiotics as well as their combination on Staphylococcus aureus. The activity of 15 plant extracts was evaluated using diffusion assay. The minimum inhibitory concentrations (MICs) and the interactions between the extracts and antibiotics as well as compound emodin were evaluated with the checkerboard method. The active extracts were a hexane extract of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotics; Bovine mastitis; Plant extract; Staphylococcus aureus.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300802

Fontes de contaminação por Staphylococcus aureus na linha de processamento de queijo prato
Provedor de dados: 45
Autores: Assumpção,E.G.; Piccoli-Valle,R.H.; Hirsch,D.; Abreu,L.R.
Com o objetivo de identificar as principais fontes de contaminação por Staphylococcus aureus e Staphylococcus produtores de coagulase (SC+), avaliou-se o processo de fabricação de queijo prato em um laticínio de Lavras, MG, durante os meses de outubro de 2000 a abril de 2001. As análises microbiológicas foram feitas no leite cru, no leite pasteurizado resfriado, nas mãos e antebraços dos funcionários, na salmoura, na água de imersão das fôrmas e no queijo embalado. Contagens elevadas de SC+ e de S. aureus (4x10³ a 4,8x10(6) UFC/ml e 4x10³ a 3,3x10(5) UFC/ml, respectivamente) foram encontradas em quatro avaliações no leite cru. Após a pasteurização, as contagens foram reduzidas a &lt;1 UFC/ml. Em três das cinco avaliações, o queijo prato apresentou...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Queijo prato; Staphylococcus aureus; Condição higienico-sanitária.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300019

An alternative method for Staphylococcus aureus DNA isolation
Provedor de dados: 45
Autores: Chapaval,L.; Moon,D.H.; Gomes,J.E.; Duarte,F.R.; Tsai,S.M.
This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Genomic DNA; DNA isolation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004

Detecção da toxina-1 da síndrome do choque tóxico em amostras de Staphylococcus aureus isoladas de mastite bovina
Provedor de dados: 45
Autores: Cardoso,H.F.T.; Carmo,L.S.; Silva,N.
Este trabalho teve por objetivo caracterizar a produção da toxina da síndrome do choque tóxico (TSST-1) e de enterotoxinas estafilocócicas (SE) A, B, C e D em 127 amostras de S. aureus, isoladas de amostras de leite proveniente de vacas com mastite no Estado de Minas Gerais, entre 1994 e 1997. A verificação da produção de toxinas foi feita pela técnica de sensibilidade ótima em placa. Das 127 amostras testadas, 60 (47%) eram produtoras de TSST-1 e 54 (43%) produtoras de SE, 38 amostras produziram SED (30%), 24 SEB (19%), 8 SEC (6%) e 4 SEA (3%). Estes resultados trazem preocupações quanto à saúde pública pela alta prevalência de amostras de S. aureus produtoras de TSST-1 e de enterotoxinas em isolamentos a partir de leite de vacas com mastite.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; TSST-1; Enterotoxina; Mastite.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000100002

Ocorrência de Staphylococcus sp. em água utilizada em propriedades leiteiras do Estado de São Paulo
Provedor de dados: 45
Autores: Amaral,L.A.; Rossi Júnior,O.D.; Nader Filho,A.; Ferreira,F.L.A.; Barros,L.S.S.
Com o objetivo de verificar o papel da água utilizada durante a produção do leite como via de transmissão de Staphylococcus sp., fez-se a contagem de Staphylococcus coagulase negativa e Staphylococcus aureus nas amostras de água das fontes, dos reservatórios e dos estábulos de 30 propriedades leiteiras situadas na região Nordeste do Estado de São Paulo. As maiores ocorrências de isolamentos (10,0 e 16,6%) e os maiores valores médios (4,3×10(4) e 2,5×10(4)) de contagens desses microrganismos foram encontrados nas amostras de água dos estábulos utilizada na obtenção de leite. Estes resultados são importantes pois evidenciam a possibilidade de contaminação do leite ou dos animais por cepas patogênicas.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Água; Staphylococcus sp.; Staphylococcus aureus; Propriedade leiteira.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000500017

Viabilidade de Staphylococcus aureus FRI S-6 e produção de SEB em queijo elaborado com adição de Lactobacillus rhamnosus e Lactococcus lactis
Provedor de dados: 45
Autores: Seridan,B.; Souza,M.R.; Nicoli,J.R.; Carmo,L.S.; Menezes,L.D.M.; Oliveira,D.L.S.; Andrade,E.H.P.
Staphylococcus aureus foi inoculado em queijos produzidos de forma estéril em laboratório, juntamente com Lactobacillus rhamnosus e Lactococcus lactis, isolados de queijo de coalho artesanal e identificados por PCR-ARDRA16S-23S. L. lactis foi capaz de reduzir a contagem de S. aureus no primeiro dia após produção (P&lt;0,05) dos queijos de 3,3x10(7)UFC/g para 1,0x10(7)UFC/g. L. rhamnosus não impediu o crescimento de S. aureus. A presença das cepas acidoláticas, principalmente L. lactis, mostrou ainda potencial de inibição da produção de enterotoxina estafilocócica do tipo B, sendo que a concentração de enterotoxinas no 15° dia foi inferior ao limite de detecção pelo kit comercial utilizado. Concluiu-se que a presença das bactérias acidoláticas estudadas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Queijo; Staphylococcus aureus; Lactococcus lactis; Lactobacillus rhamnosus; Enterotoxinas; Qualidade sanitária.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200029

Caracterização de biótipos de Staphylococcus aureus isolados de mastite bovina
Provedor de dados: 45
Autores: Brito,M.A.V.P.; Brito,J.R.F.; Cordeiro,F.M.; Costa,W.A.; Fortes,T.O.
Duzentos e dezoito amostras de Staphylococcus aureus, isoladas de infecção intramamária de vacas de 44 rebanhos leiteiros, foram classificadas em biótipos de acordo com os testes de produção de estafiloquinase (K), beta-hemolisina (beta ), coagulação do plasma bovino (Pl) e crescimento na presença de cristal violeta (CV). As amostras foram distribuídas em 10 biótipos e 63 delas foram classificadas nas ecovariedades bovina (35), ovina (17), aviária (10) e humana (1) e 155 não apresentaram características específicas de hospedeiro. Estas últimas podem ser isoladas de homem, cabra, coelho, suíno, alimentos e de mastite bovina. O biótipo 1, encontrado com maior freqüência (37,2%), apresentou o padrão K (-), beta (+), Pl (-) e CV (azul). Em sete rebanhos nos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Mastite; Staphylococcus aureus; Biótipo.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000500001

Produção de enterotoxinas e da toxina da síndrome do choque tóxico por cepas de Staphylococcus aureus isoladas na mastite bovina
Provedor de dados: 45
Autores: Nader Filho,A.; Ferreira,L.M.; Amaral,L.A.; Rossi Junior,O.D.; Oliveira,R.P.
A total of 72 strains of Staphylococcus aureus were examined for the production of staphylococcal enterotoxins (SE) A, B, C, D and toxic shock syndrome toxin (TSST-1). The strains were isolated from milk samples from cows with mastitis in dairy herds of São Paulo State, Brazil. Off 72 isolates, 38 (52.8%) produced SEA, 38 (52.8%) SEB, 32 (44.4%) SED, 28 (38.9%) SEC and 27 (37.5%) TSST-1. From the 72 strains, 66 (91.7%) produced, at least, one or more toxin, including TSST-1.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Mastite; Staphylococcus aureus; Enterotoxinas.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500032

Biocompatibilidade e biodegradabilidade de micropartículas de poli-lactato-co-glicolato na ceratite infecciosa em coelhos
Provedor de dados: 45
Autores: Ranzani,J.J.T.; Peixoto,T.P.; Brandão,C.V.S.; Oliveira,A.G.; Cunha,M.L.R.S.; Mori,E.S.; Minto,B.W.
Avaliaram-se a biocompatibilidade e a biodegradabilidade do sistema de liberação controlada de poli-lactato-co-glicolato (PLGA) no tratamento com ciprofloxacina das ceratites por Staphylococcus aureus em coelhos. Foram utilizados 20 coelhos, distribuídos em quatro grupos (G). Os animais dos G1, G3 e G4 foram inoculados com 2,5µL da bactéria - 108UFC, no estroma corneano. Os do G2 não receberam a aplicação do inóculo. O tratamento foi realizado com solução salina básica para os animais do G1, micropartículas de PLGA contendo ciprofloxacina nos animais dos G2 e G4 e colírio de ciprofloxacina naqueles do G3. Suabe e biópsia da superfície ocular foram coletados para cultura. Apenas um animal do G1 apresentou cultura positiva para S. aureus. Exame histológico...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coelho; Sistema de liberação controlada; Ceratite; Poli-lactato-co-glicolato; Staphylococcus aureus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000500013

Efeitos da administração de vitamina E na infecção mamária e na contagem de células somáticas de cabras primíparas desafiadas experimentalmente com Staphylococcus aureus
Provedor de dados: 45
Autores: Paes,P.R.O.; Lopes,S.T.A.; Lopes,R.S.; Kohayagawa,A.; Takahira,R.K.; Langoni,H.
O trabalho teve por objetivo estudar os efeitos da administração de vitamina E sobre a contagem de células somáticas e a infecção da glândula mamária de cabras primíparas desafiadas com a inoculação intramamária de Staphylococcus aureus ao 10º dia pós-parto. Vinte e oito animais foram divididos em quatro grupos, cada um composto por sete cabras primíparas da raça Saanen, como segue: grupo-controle, grupo de animais suplementados com vitamina E, grupo de animais desafiados com S.aureus inoculados na glândula mamária e grupo de animais suplementados com vitamina E e desafiados com S.aureus na glândula mamária. Na segunda e terceira semanas de lactação, a inoculação de S.aureus na glândula mamária permitiu a recuperação do microrganismo no leite e elevou a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caprino; Mastite; Staphylococcus aureus; Contagem de células somáticas; Vitamina E.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000100003

Resistência bacteriana e ação das bacteriocinas de Lactobacillus spp em Staphylococcus aureus isolados de mastite bovina
Provedor de dados: 45
Autores: Pribul,B.R.; Pereira,I.A.; Soares,L.C.; Coelho,S.M.O.; Barberis,I.L.; Pascual,L.; Souza,M.M.S.
Staphylococcus aureus is the major pathogen causing intramammary infections in dairy cattle worldwide. Among the factors that contribute to its spread and infectious potential is the ability to overcome the mechanisms of antimicrobials activity. The present work investigated the antimicrobial resistance pattern and sensibility to bacteriocins produced by strains of Lactobacillus spp of 30 isolates of S. aureus from mastitis. From this, 29 are beta-lactamase producers. Eight isolates (26.6%) showed resistance to at least four antibiotics being considered multiresistent. All of them were mecA-positive. Otherwise, all isolates tested showed sensibility to at least one of the four bacteriocin producer strains. Due to the significant depletion of the efficacy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Bovine mastitis; Antimicrobial resistance; Bacteriocins; Lactobacillus spp.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300029

Produção de biocompostos com atividade antimicrobiana de Streptomyces sp. ante isolados de mastite caprina
Provedor de dados: 45
Autores: Nascimento,T. Pajeú; Porto,C.S.; Teixeira,M.F.S.; Porto,T.S.; Porto,A.L.F.
Actinomicetos são um dos principais produtores de enzimas, vitaminas e metabólitos secundários, destacando-se o gênero Streptomyces, o qual tem uma ampla capacidade de produção de antibióticos eficazes no combate a diferentes microrganismos, entre eles o Staphylococcus sp. Em virtude dessa eficiência no combate a patógenos, o objetivo deste trabalho foi avaliar a produção de metabólitos com atividade antimicrobiana produzidos por 67 Streptomyces isolados de liquens da região amazônica, ante isolados de mastite caprina (Staphylococcus aureus) do estado de Pernambuco, Brasil. Foi utilizado um planejamento fatorial para avaliar a influência das fontes de carbono (glicose) 0%, 0,5% e 1% e de nitrogênio (farinha de soja) 1%, 2,5% e 4% na produção dos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cabra; Actinomicetos; Staphylococcus aureus; Mastite.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000100015

Mastite subclínica causada por Staphylococcus aureus: custo-benefício da antibioticoterapia de vacas em lactação
Provedor de dados: 45
Autores: Zafalon,L.F.; Nader Filho,A.; Oliveira,J.V.; Resende,F.D.
Avaliou-se a relação custo-benefício do tratamento da mastite subclínica bovina causada por Staphylococcus aureus. Foram selecionados 270 quartos mamários com mastite subclínica e sadios, divididos em quatro grupos de acordo com o estádio de lactação e o tratamento. O grupo 1 foi formado por animais entre 10 e 60 dias da lactação e tratados contra mastites; o grupo 2 incluiu animais entre 61 dias da lactação e dois meses antes da secagem e tratados contra mastite; o grupo 3 foi formado por animais entre 10 e 60 dias da lactação, não tratados contra mastite; e o grupo 4 foi formado por animais entre 61 dias em lactação e dois meses antes da secagem, não tratados. O tratamento foi realizado pela infusão intramamária de 150mg de gentamicina, uma vez ao dia. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Mastite subclínica; Tratamento; Antibiótico; Staphylococcus aureus; Custos.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300005

Characterization of virulence and antibiotic profile and agr typing of Staphylococcus aureus from milk of subclinical mastitis bovine in State of Rio de Janeiro
Provedor de dados: 45
Autores: Soares,B.S.; Melo,D.A.; Motta,C. C.; Marques,V.F.; Barreto,N.B.; Coelho,S.M.O.; Coelho,I.S.; Souza,M.M.S.
ABSTRACT This study aims to detect the main virulence and antimicrobial resistance genes in Stapylococcus aureus from bovine mastitic milk as well as classifying them according to agr typing. A total of 55 strains from six dairy unities in the state of Rio de Janeiro were selected, of these 27.3% presented fbnA and 78,2% for fbnB genes, respectively. None of the strains tested were positive for cap5 gene, 3.6% were positive for cap8 gene. Additionally, 94.5% of strains had hlA gene and 89.1% had hlB gene while 67.3% of the strains had icaA gene and 87.3% had icaD gene. From these results it was possible to establish 12 different virulence profiles. Prevalence of agrII type was detected in 81.8% of the isolates. Concerning antimicrobial resistance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agr typing antibiotics; Bovine mastits; Staphylococcus aureus; Virulenc.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000400843

Actinomicetos produtores de inibidores de β-lactamases com atividade antimicrobiana frente a isolados de mastite bovina
Provedor de dados: 45
Autores: Cunha,M.N.C; Silva,N.M.V; Teixeira,M.F.S; Mota,R.A; Lima-Filho,J.L; Porto,T.S; Porto,A.L.F.
Avaliou-se a capacidade de 71 actinomicetos isolados de líquens da região amazônica em produzir inibidores de β-lactamases com atividade antimicrobiana sobre Staphylococcus aureus, resistentes à penicilina, isolados de mastite bovina do estado de Pernambuco. A seleção dos actinomicetos produtores de inibidores de β-lactamases foi realizada pela técnica de bloco de gelose contra Klebsiella pneumoniae ATCC 29665, e os actinomicetos selecionados foram testados frente a 17 linhagens de Staphylococcus aureus resistentes à penicilina. Os melhores produtores de inibidores de β-lactamases foram Streptomyces sp. DPUA 1542 e Nocardia sp. DPUA 1571, os quais foram submetidos ao cultivo submerso para determinação da curva de crescimento, pH e atividade antimicrobiana....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastite bovina; Inibidor de β-lactamase; Actinomiceto; Atividade antimicrobiana; Staphylococcus aureus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600003

Concentração mínima inibitória de dez antimicrobianos para amostras de Staphylococcus aureus isoladas de infecção intramamária bovina
Provedor de dados: 45
Autores: Brito,M.A.V.P.; Brito,J.R.F.; Silva,M.A.S.; Carmo,R.A.
Determinou-se a concentração mínima inibitória (CMI) de ampicilina, cefalotina, eritromicina, gentamicina, neomicina, norfloxacina, oxacilina, penicilina G, tetraciclina e tilosina para 112 amostras de Staphylococcus aureus isoladas de infecções intramamárias bovina, em 33 rebanhos leiteiros da Zona da Mata do Estado de Minas Gerais. Foram isoladas 24 amostras de infecção clínica, 66 de subclínica e 22 de infecção crônica. As amostras de infecção crônica foram isoladas repetidas vezes dos mesmos quartos mamários de nove vacas de um rebanho, no período de 13 meses. A CMI foi realizada em ágar Mueller Hinton, com concentrações entre 0,015 e 128µgml-1 de cada antimicrobiano. As amostras da American Type Culture Collection (ATCC) recomendadas para controle de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastite bovina; Staphylococcus aureus; Susceptibilidade antimicrobiana; Concentração mínima inibitória; Beta-lactamase.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000500003

Detecção dos genes de Staphylococcus aureus, enterotoxinas e de resistência à meticilina em leite
Provedor de dados: 45
Autores: Dias,N.L.; Silva,D.C.B.; Oliveira,D.C.B.S.; Fonseca Junior,A.A.; Sales,M.L.; Silva,N.
Realizou-se a detecção do gene de Staphylococcus aureus, de enterotoxinas e de resistência à meticilina com extração de DNA feita diretamente de amostras de leite. Das 200 amostras estudadas, 145 (72,5%) amplificaram o gene femA, e estas foram analisadas quanto à presença dos genes sea, seb, sec e mecA. Os genes das enterotoxinas mais prevalentes foram: sea (60%), seb (37,9%) e sec (6,9%). Foram encontradas 18 amostras de leite (11,0 %) com S. aureus portadores do gene mecA. A detecção de S. aureus diretamente do leite, sem a necessidade de isolamento bacteriano e a caracterização do potencial enterotoxigênico, demonstra que a técnica de PCR é muito útil para estudos epidemiológicos das infecções estafilocócicas da glândula mamária. O alto percentual...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Enterotoxinas; MRSA; PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600036

Bovine mastitis in the metropolitan area of Curitiba: antibiotic resistance and antimicrobial control of the infection
Provedor de dados: 52
Autores: Parada,José Luis; Gonçalves,Dicezar; Soccol,Vanete Tomaz; Lima,Marisa; Soccol,Carlos Ricardo.
A study from cows with mastitis was performed and Staphylococcus aureus was the predominant pathogen in 46.4 % among 153 studied strains from 276 milk samples of infected cows. Antibiotic resistance of 71 S. aureus isolates was determined in order to search resistant strains to antibiotics of clinical interest, as well as to determine their degree of multi-resistance. It was found that 60% of the S. aureus strains presented resistance to β-lactams, but none to oxacillin, teicoplamin or vancomycin. On the other hand, with the aim of reducing the use of current antibiotics and their associated resistance, a new formulation was introduced. The antimicrobial compounds (P22-P32), demonstrated to be effective in 55% of the 76 mastitis cases studied. The use of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Staphylococcus aureus; Antibiotic resistance; Infection reduction.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400009

New method for early detection of two random amplified polymorphic DNA (RAPD) groups of Staphylococcus aureus causing bovine mastitis infection in Paraná State, Brazil
Provedor de dados: 52
Autores: Gonçalves,Dicezar; Gabriel,Jane Eire; Madeira,Humberto Maciel França; Schühli,Guilherme Schnell e; Vicente,Vânia Aparecida.
The aim of this work was to develop a fast and accurate molecular approach to allow early detection of two RAPD groups of S. aureus causing bovine mastitis. Seventy five S. aureus isolates from infected animals were characterized by RAPD. Genomic fragments isolated from the unique bands present in either group were cloned and sequenced. Based on the DNA sequences, specific primers were designed to allow for the simultaneous detection of either group by multiplex PCR of S. aureus DNA isolated from clinical and subclinical bovine mastitis. Results showed that these proposed primers set could be used to detect various clinical and subclinical S. aureus isolates as well as the detection of the microorganism in bulk milk. Their use as a specific method for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Molecular markers; Subclinical mastitis; Clinical mastitis; Mastitis detection.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200014

Antimicrobial activity of a biosurfactant produced by Bacillus licheniformis strain M104 grown on whey
Provedor de dados: 52
Autores: Gomaa,Eman Zakaria.
The aim of the present study was to investigate the antimicrobial effect of the lipopeptide biosurfactants produced by Bacillus licheniformis strain M104 grown on whey. The biosurfactant was investigated for potential antimicrobial activity by using the disc-diffusion method against different Gram-positive bacteria {B subtilis, B. thuringiensis (two strains), B. cereus, Staphylococcus aureus (two strains) and Listeria monocytogenes)}, Gram-negative bacteria {(Pseudomonas aeruginosa, Escherichia coli (two strains), Salmonella typhimurium, Proteous vulgaris and Klebsiella pneumoniae) and a yeast (Candida albicans)}. The biosurfactant showed profoundly distinct antibacterial activity toward tested bacteria and displayed an antifungal activity against the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosurfactant; Bacillus licheniformis; Cheese whey; Antimicrobial activity; Staphylococcus aureus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200011

Importância do Staphylococcus aureus nas mastites subclínicas: pesquisa de enterotoxinas e toxina do choque tóxico, e a relação com a contagem de células somáticas
Provedor de dados: 62
Autores: Sá,Marcos Eielson Pinheiro de; Cunha,Maria de Lourdes Ribeiro de Souza da; Elias,Acacia Orieth; Victória,Cassiano; Langoni,Helio.
O Staphylococcus aureus é um dos principais agentes das mastites consideradas contagiosas, apresentando elevada incidência na maioria dos rebanhos leiteiros em vários países. Além de perdas econômicas é importante salientar o aspecto de saúde pública para cepas produtoras de enterotoxinas e da toxina do choque tóxico. A enterotoxina A, relacionada com maior ênfase nos casos de toxinfecções alimentares, pode ser veiculada pelo leite cru, pasteurizado e subprodutos lácteos. A síndrome do choque tóxico é determinada mais freqüentemente pela toxina do choque tóxico, porém as enterotoxinas do tipo B e C também podem ser implicadas. O objetivo deste estudo foi verificar a ocorrência de S.aureus produtores de enteroxinas e da toxina do choque tóxico em amostras...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastite; Subclinica; Bovino; Staphylococcus aureus; Enterotoxinas.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000500005

Antimicrobial susceptibility, virulence determinant carriage and molecular characteristics of Staphylococcus aureus isolates associated with skin and soft tissue infections
Provedor de dados: 55
Autores: Yu,Fangyou; Liu,Yunling; Lv,Jinnan; Qi,Xiuqin; Lu,Chaohui; Ding,Yu; Li,Dan; Liu,Huanle; Wang,Liangxing.
ABSTRACT A better understanding of the antimicrobial susceptibility, carriage of virulence determinants and molecular characteristics of Staphylococcus aureus isolates associated with skin and soft tissue infections (SSTIs) may provide further insights related to clinical outcomes with these infections. From January 2012 to September 2013, a total of 128 non-duplicateS. aureus isolates were recovered from patients with SSTIs. All 128 S. aureus SSTI isolates carried at least five virulence genes tested. Virulence genes detected among at least 70% of all tested isolates included hld (100%), hla (95.3%),icaA (96.9%), clf (99.2%),sdrC (79.7%), sdrD (70.3%), andsdrE (72.7%). The prevalence of MRSA isolates with 10 virulence genes tested (54.4%, 31/56) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Skin and soft tissue infections; Molecular characteristic; Virulence genes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600614

Detection of the mecA gene and identification of Staphylococcus directly from blood culture bottles by multiplex polymerase chain reaction
Provedor de dados: 55
Autores: Rocchetti,Taisa Trevizani; Martins,Katheryne Benini; Martins,Patricia Yoshida Faccioli; Oliveira,Rogério Antonio de; Mondelli,Alessandro Lia; Fortaleza,Carlos Magno Castelo Branco; Cunha,Maria de Lourdes Ribeiro de Souza da.
ABSTRACT  Introduction: Staphylococcus spp. – both S. aureus, including methicillin-resistant strains (MRSA) and coagulase negative staphylococci (CoNS) – are relevant agents of healthcare-associated infections. Therefore, the rapid recognition of MRSA and methicillin-resistant CoNS from blood stream infections is critically important for patient management. It is worth noting that inappropriate empiric therapy has been associated with higher in-hospital mortality.  Material and methods: In this study we evaluated a multiplex polymerase chain reaction (multiplex PCR) standardized to detect Staphylococcus spp., S. aureus, and mecA gene-encoded oxacillin resistance directly from blood culture bottles. A total of 371 blood cultures with Gram-positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus spp.; Staphylococcus aureus; MRSA; MecA gene; Blood cultures; Multiplex PCR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200099

Is community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) an emerging pathogen among children in Brazil?
Provedor de dados: 55
Autores: Paternina-de la Ossa,Rolando; Prado,Seila Israel do; Cervi,Maria Célia; Lima,Denissani Aparecida Ferrari dos Santos; Martinez,Roberto; Bellissimo-Rodrigues,Fernando.
ABSTRACT  Background: Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is spreading worldwide, but little is known about the epidemiology of this pathogen in Brazil.  Objective: To evaluate clinical and microbiological features of children with S. aureus infections admitted to a university hospital.  Methods: This was a cross-sectional study evaluating the potential risk factors for CA-MRSA, and a retrospective cohort evaluating in-hospital clinical outcomes. To include patients with both community and hospital-associated infections, we screened the results of the microbiological laboratory tests from January 1, 2012, to December 31, 2016. According to the phenotype, we classified the isolates in Methicillin-Susceptible S. aureus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; CA-MRSA; Epidemiology; Clinical features; Children; Susceptibility profile.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000500371

Infection and colonization by Staphylococcus aureus in a high risk nursery of a Brazilian teaching hospital
Provedor de dados: 55
Autores: Silva,Helisângela de Almeida; Abdallah,Vânia O. Steffen; Carneiro,Cláudia Lúcia; Gontijo Filho,Paulo P.
Neonates are susceptible to nosocomial infections due to immunological immaturity, prolonged hospital stay and the use of invasive procedures. We evaluated the incidence of infections and the prevalence of colonization by MRSA (Methicillin-resistant Staphylococcus aureus) and MSSA (Methilin-susceptible Staphylococcus aureus), as well as colonization risk factors. Staphylococcal infections were observed by analyzing medical records in the HICS (Hospital Infection Control Service) and the HRN (High Risk Nursery). Additionally, four inquiries concerning colonization prevalence were made for S. aureus, from January/2000 to December/2002. Clinical specimens from the nostrils, mouth and anus were cultivated in mannitol-salt agar plates and identification was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Neonates; Infection; Colonization and risk factors.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000600005

Length of exposure to the hospital environment is more important than antibiotic exposure in healthcare associated infections by methicillin-resistant Staphylococcus aureus: a comparative study
Provedor de dados: 55
Autores: Baraboutis,Ioannis G; Tsagalou,Eleftheria P; Papakonstantinou,Ilias; Marangos,Markos N; Gogos,Charalambos; Skoutelis,Athanasios T; Bassaris,Haralambos; Johnson,Stuart.
OBJECTIVES: Both total antimicrobial use and specific antimicrobials have been implicated as risk factors for healthcare-associated methicillin-resistant Staphylococcus aureus (HCA-MRSA) infection. The aims of this study were: (I) to explore predictors of a new HCA-MRSA infection in comparison with a new healthcare-associated methicillin-sensitive Staphylococcus aureus (HCA-MSSA); (II) to thoroughly assess the role of recent antibiotic use qualitatively and quantitatively. METHODS: The time-period for our study was from October 1997 through September 2001. Through applying strict criteria, we identified two groups of inpatients, one with a new HCA-MRSA infection and one with a new HCA-MSSA infection. We recorded demographic, clinical and antibiotic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methicillin-resistant Staphylococcus aureus; Staphylococcus aureus; Antibacterial agents; Fluoroquinolones.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500003

Cost-effectiveness of linezolid versus vancomycin in mechanical ventilation-associated nosocomial pneumonia caused by methicillin-resistant staphylococcus aureus
Provedor de dados: 55
Autores: Machado,Adão R. L.; Arns,Clovis da Cunha; Follador,Wilson; Guerra,Aline.
Linezolid, an oxazolidinone-class antimicrobial agent, is a new drug; its use has frequently been questioned due to its high price. However, recent trials have demonstrated that the use of linezolid in mechanical ventilation-associated nosocomial pneumonia caused by methicillin-resistant Staphylococcus aureus (VAP-MRSA) may be justified due to its improved efficacy compared to vancomycin. Price and cost have different magnitudes, and clinical efficacy should always be considered in the decision-making process. Our objective was to determine whether linezolid treatment was more cost-effective than vancomycin for treating VAP-MRSA. METHODOLOGY: Elaboration of an economic model from a metanalysis of previous clinical trials comparing both drugs, through a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Linezolid; Vancomycin; Staphylococcus aureus; Pneumonia; Ventilator; Cost; Pharmacoeconomic.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000300001

Antibacterial resistance of community-acquired respiratory tract pathogens recovered from patients in Latin America: results from the PROTEKT surveillance study (1999-2000)
Provedor de dados: 55
Autores: Mendes,C.; Marin,M. E.; Quiñones,F.; Sifuentes-Osornio,J.; Cuilty Siller,C.; Castanheira,M.; Zoccoli,C. M.; López,H.; Súcari,A.; Rossi,F.; Barriga Angulo,G.; Segura,A.J.A.; Starling,C.; Mimica,I.; Felmingham,D.
PROTEKT (Prospective Resistant Organism Tracking and Epidemiology for the Ketolide Telithromycin) is a global surveillance study established in 1999 to monitor antibacterial resistance of respiratory tract organisms. Thirteen centers from Argentina, Brazil and Mexico participat ed during 1999-2000; they collected 1,806 isolates (Streptococcus pneumoniae 518, Haemophilus influenzae 520, Moraxella catarrhalis 140, Staphylococcus aureus 351, S. pyogenes 277). Overall, 218 (42.1%) of the S. pneumoniae isolates had reduced susceptibility to penicillin, 79 (15.3%) were penicillin-resistant and 79 (15.3%) were erythromycin-resistant. Mexico had the highest prevalence of penicillin (76.5%) and erythromycin (31.2%) resistance. Of 77 erythromycin-resistant S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PROTEKT; Latin America; Resistance; Respiratory tract infections; Streptococcus pneumoniae; Haemophilus influenzae; Moraxella catarrhalis; Streptococcus pyogenes; Staphylococcus aureus.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000100006

Changes in Staphylococcus aureus susceptibility across Latin America between 2004 and 2010
Provedor de dados: 55
Autores: Garza-González,Elvira; Dowzicky,Michael Joseph.
The Tigecycline Evaluation and Surveillance Trial is a global surveillance study monitoring the efficacy of tigecycline and comparators against clinically important pathogens. Between 2004 and 2010, 3126 isolates of Staphylococcus aureus were collected from 66 centers in 13 countries in Latin America; of these, 1467 (46.9%) were resistant to methicillin. The main contributors of S. aureus isolates were Mexico (n = 846), Argentina (n = 740), and Colombia (n = 445). The methicillin-resistant S. aureus rate was greater than 50% in five countries, the highest reported in Puerto Rico (73.9%). Methicillin-resistant S. aureus rates across Latin America ranged from 40.1% to 50.6% over the study period. All S. aureus isolates were susceptible to linezolid and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tigecycline; Antimicrobial surveillance; Latin America; Staphylococcus aureus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000100003

Antibacterial effect of borage (Echium amoenum) on Staphylococcus aureus
Provedor de dados: 55
Autores: Abolhassani,Mohsen.
Borage (Echium amoenum) is a large annual plant of the Boraginaceae family, which grows in most of Europe and in northern Iran. The borage flower is used as a medicinal herb in France and other countries. Iranian borage is used in traditional medicine for infectious diseases, flu and as an anti-febrile. We tested the aqueous extract of borage dried flowers in vitro for its antibacterial activity. The extract showed concentration-dependent antibacterial activity against Staphylococcus aureus 8327. This activity was heat resistant, but the activity of freeze-dried extract gradually diminished during a 90-day period. The traditional use of Iranian borage flowers for infectious diseases and for controlling fever appears to be justified.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Borago; Echium amoenum; Antibacterial activity; Staphylococcus aureus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500008

Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) among patients visiting the emergency room at a tertiary hospital in Brazil
Provedor de dados: 55
Autores: Ribeiro,Julival; Boyce,John M.; Zancanaro,Pedro Q.
We surveyed patients seen at the emergency room at a tertiary hospital in Brazil from August to November 1997. All patients' (n = 600) anterior nares were cultured for MRSA; the results were confirmed by oxacillin disk diffusion methods and by detection of the mecA gene with PCR. Bacteria were found in 93.3% (560) of the patients and MRSA in 0.7% (n = 4). One patient had community-acquired MRSA. We concluded that MRSA is uncommon among patients visiting the emergency room. The presence of community-acquired MRSA can lead to serious medical and epidemiological issues, although initial clinical presentation may not differ from that of infections with other staphylococci. The empirical use of vancomycin for suspected community-acquired infections is seldom...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MRSA; Staphylococcus aureus; Emergency room; Brazil.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100009

Community-acquired methicillin-resistant Staphylococcus aureus from skin and soft tissue infections (in a sample of Egyptian population): analysis of mec gene and staphylococcal cassette chromosome
Provedor de dados: 55
Autores: Sobhy,Nagat; Aly,Fatma; El Kader,Ola Abd; Ghazal,Abeer; Elbaradei,Amira.
BACKGROUND: Staphylococcus aureus has been recognized as an important pathogen associated with inpatients and community infections. Community-acquired methicillin-resistant S. aureus (CA-MRSA) infections commonly present as skin and soft-tissue infections (SSTIs). Treatment often includes incision and drainage with or without adjunctive antibiotics. OBJECTIVES: This study aimed to identify CA-MRSA infections both phenotypically and genotypically, to determine their spectrum of antibiotic resistance, and to establish the best scheme for molecular distinction between hospital-acquired MRSA (HA-MRSA) and CA-MRSA by staphylococcal cassette chromosome mec (SCCmec) typing and detection of Panton Valentine leukocidin (PVL). MATERIALS: 50 swabs, from skin and soft...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Community-acquired; Methicillin-resistant; Staphylococcus aureus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500004

Prevalence and antimicrobial susceptibility of respiratory pathogens in patients with cystic fibrosis
Provedor de dados: 55
Autores: Paixão,Vilma Almeida; Barros,Tânia Fraga; Mota,Clélia Maria C; Moreira,Tamy Fagundes; Santana,Maria Angélica; Reis,Joice Neves.
Respiratory infection is very common in patients suffering from cystic fibrosis (CF). However, the antimicrobial resistance rate of isolates from CF patients is not often documented. In this study, 279 respiratory specimens of 146 patients were prospectively collected from July to December 2006. Microbiological cultures and antimicrobial susceptibility tests of the most frequently isolated bacteria were performed. Sputum and oropharyngeal swabs were processed for culture. During the study period, 50% of the patients harbored Staphylococcus aureus, 35% Pseudomonas aeruginosa, 4.7% Haemophilus influenzae. Methicillin resistant S. aureus (MRSA) were detected in 8 (6%) patients; ESBL and MBL-producing P. aeruginosa were not identified in these patients. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cystic fibrosis; Antimicrobial resistance; Pseudomonas aeruginosa; Staphylococcus aureus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400016

Staphylococcus aureus regulates secretion of interleukin-6 and monocyte chemoattractant protein-1 through activation of nuclear factor kappaB signaling pathway in human osteoblasts
Provedor de dados: 55
Autores: Ning,Rende; Zhang,Xianlong; Guo,Xiaokui; Li,Qingtian.
OBJECTIVE: Activation of nuclear factor kappaB by diverse bacteria regulates the secretion of chemokines and cytokines. Staphylococcus aureus (S. aureus)-infected osteoblasts can significantly increase the secretion of interleukin-6 and monocyte chemoattractant protein-1. The aim of this study was to investigate whether S. aureus can activate nuclear factor kappaB in human osteoblasts, and whether the activation of nuclear factor kappaB by S. aureus regulates the secretion of interleukin-6 and monocyte chemoattractant protein-1. METHODS: Immunoblot and electrophoretic mobility shift assay were used to detect the degradation of IκBa and activation of nuclear factor kappaB in human osteoblasts in response to S. aureus, respectively. Enzyme-linked...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Osteoblasts; NF-kappaB; Interleukin-6; Chemokine CCL2.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300001

Healthcare-associated Staphylococcus aureus bloodstream infection: length of stay, attributable mortality, and additional direct costs
Provedor de dados: 55
Autores: Primo,Mariusa Gomes Borges; Guilarde,Adriana Oliveira; Martelli,Celina M. Turchi; Batista,Lindon Johnson de Abreu; Turchi,Marília Dalva.
This study aimed to determine the excess length of stay, extra expenditures, and attributable mortality to healthcare-associated S. aureus bloodstream infection (BSI) at a teaching hospital in central Brazil. The study design was a matched (1:1) case-control. Cases were defined as patients &amp;gt; 13 years old, with a healthcare-associated S. aureus BSI. Controls included patients without an S. aureus BSI, who were matched to cases by gender, age (± 7 years), morbidity, and underlying disease. Data were collected from medical records and from the Brazilian National Hospital Information System (Sistema de Informações Hospitalares do Sistema Único de Saúde - SIH/SUS). A Wilcoxon rank sum test was performed to compare length of stay and costs between cases...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Bloodstream infection; Attributable mortality; Length of stay; Extra costs.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600001

High frequency of colonization and absence of identifiable risk factors for methicillin-resistant Staphylococcus aureus (MRSA) in intensive care units in Brazil
Provedor de dados: 55
Autores: Korn,Gustavo P.; Martino,Marinês D. V.; Mimica,Igor M.; Mimica,Lycia J.; Chiavone,Paulo A.; Musolino,Luiz R. de S.
Colonization of hospitalized patients with methicillin-resistant Staphylococcus aureus (MRSA) is of increasing concern. To evaluate this problem in Intensive Care Units (ICUs) in Brazil, we studied 100 patients admitted to two ICUs from April to June, 1997. Of the 100 patients, 70 were male, 53 were age 60 years or older, 55 were previously hospitalized, 78 were transferred to the ICU from other hospital units, 49 had received antibiotic therapy, and 66 had undergone recent surgery. Nasal and axillary swab cultures were obtained on admission and every 48 hours thereafter until discharge. MRSA were identified by plating any cultured S. aureus on Mueller-Hinton agar containing 6mug/ml of oxacillin. At the time of admission, 46 (46%) of the patients were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Methicillin resistance; Hospital infections.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000100001

Prevalence and risk factors for Staphylococcus aureus in health care workers at a University Hospital of Recife-PE
Provedor de dados: 55
Autores: Silva,Eduardo Caetano Brandão Ferreira da; Antas,Maria das Graças C.; B. Neto,Armando Monteiro; Rabelo,Marcelle Aquino; Melo,Fábio Lopes de; Maciel,Maria Amélia Vieira.
Staphylococcus aureus is the main human pathogen that colonizes individuals in general population. The objective of the study was evaluate the epidemiological and sensitivity profile of S. aureus lineage, isolated in health care workers (HCW) of a University Hospital in Pernambuco state, Brazil. Biological samples of hands and nasal cavities were sown in agar sheep blood. Colonies under suspicion of being S. aureus were identified using Gram staining, catalase test and coagulase, mannitol-salty agar fermentation and DNAse agar. The resistance to mupirocin was analyzed through the Kirby Bauer technique. In relation to methicillin and vancomycin the determination was by the minimum inhibitory concentration method (E-test). From the 202 HCW evaluated, 52 were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Colonization; Health staff.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600012

Associated risk factors and pulsed field gel electrophoresis of nasal isolates of Staphylococcus aureus from medical students in a tertiary hospital in Lagos, Nigeria
Provedor de dados: 55
Autores: Adesida,Solayide A.; Abioye,Olusegun A.; Bamiro,Babajide S.; Brai,Bartholomew I.C.; Smith,Stella I.; Amisu,Kehinde O.; Ehichioya,Deborah U.; Ogunsola,Folasade T.; Coker,Akitoye O.
Staphylococcus aureus infections are growing problems worldwide with important implications in hospitals. The organism is normally present in the nasal vestibule of about 35% of apparently healthy individuals and its carriage varies between different ethnic and age groups. Staphylococcal nasal carriage among health workers is particularly important to establish new clones and track origin of infections during outbreak situations. To determine the carriage rate and compare the pulsed field gel patterns of the strains, nasal swabs were collected from 185 medical students in a teaching hospital in Lagos, Nigeria. Isolates of S. aureus were tested for heamolysin production, methicillin sensitivity and Pulsed Field Gel Electrophoresis (PFGE) was performed. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; PFGE; Nasal isolates; Carriage rate.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100016

Temporal evolution of the prevalence of methicillin-resistant Staphylococcus aureus in a tertiary hospital in Bahia, Brazil: a nine-year evaluation study
Provedor de dados: 55
Autores: Brites,Carlos; Silva,Nanci; Sampaio- Sá,Márcia.
Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have become an increasing problem in Brazilian hospitals within the last years. In Bahia, there is scarce information on the epidemiological characteristics of MRSA infections and their determinants. The objective of this study was to evaluate the temporal evolution of MRSA infections in a private, tertiary hospital, in Salvador, the capital of the state of Bahia. We reviewed the microbiological records of bacterial isolates from the Hospital Espanhol, a 300 bed, general hospital, in Salvador. We analyzed the frequency of positive cultures for S. aureus during the last nine years, the prevalence of MRSA, and the spatial distribution of the isolates in the hospital. We also evaluated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; MRSA; Bacterial resistance.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000400003

Lemierre syndrome from a neck abscess due to methicillin-resistant Staphylococcus aureus
Provedor de dados: 55
Autores: Abhishek,Agarwal; Sandeep,Singla; Tarun,Pandey.
Lemierre syndrome is characterized by acute septic thrombophlebitis of the internal jugular vein (IJV) that develops after an oropharyngeal infection, and can be complicated by septic emboli to lungs and other organs. The most frequent causative agent is Fusobacterium necrophorum, an anaerobic bacillus found in normal oropharyngeal flora. Staphylococcus aureus has emerged as a cause of Lemierre syndrome in the last decade. We report a case of a 24-year-old man who developed septic IJV thrombosis and necrotizing pneumonia due to S. aureus from an infected hematoma in the right sternocleidomastoid muscle. Antibiotics are the mainstay of therapy with few cases needing anticoagulation. A good outcome is dependent upon an awareness of the condition, a high...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Internal jugular vein thrombosis; Staphylococcus aureus; Pulmonary emboli; Septic emboli.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000400024

Complete substitution of the Brazilian endemic clone by other methicillin-resistant Staphylococcus aureus lineages in two public hospitals in Rio de Janeiro, Brazil
Provedor de dados: 55
Autores: Chamon,Raiane Cardoso; Ribeiro,Sthefanie da Silva; Costa,Thaina Miranda da; Nouér,Simone Aranha; Santos,Katia Regina Netto dos.
Abstract Staphylococcus aureus is an important cause of bloodstream infections. Therefore, the main purpose of this work was to characterize a collection of 139 S. aureus isolates from bloodstream infections in two public hospitals in relation to their antimicrobial susceptibility profile, staphylococcal cassette chromosome mec types, and clonal relationship. Methicillin resistance and resistance to other 12 agents were accessed by the disk diffusion test. Minimum inhibitory concentration to mupirocin was also determined. The SCCmec types were accessed by multiplex PCR, and the clonal relationship was determined by pulsed field gel electrophoresis method and restriction modification system characterization. Besides, multilocus sequence typing was performed...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Staphylococcus aureus; Bloodstream infections; USA100; Mupirocin resistance.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000200185

Risk factors and evolution of Ventilator-associated pneumonia by Staphylococcus aureus sensitive or resistant to oxacillin in patients at the intensive care unit of a Brazilian University Hospital
Provedor de dados: 55
Autores: Moreira,Michel R.; Cardoso,Renata L.; Almeida,Alair B.; Gontijo Filho,Paulo P.
This study investigated the participation and risk factors of VAP by resistant (ORSA) or sensitive (OSSA) S. aureus to oxacillin and evaluated the implications of adequate or inadequate empirical antimicrobial therapeutics in its evolution in patients interned in a mixing ICU of adults. A patient control-case study with PAVs by ORSA and OSSA was carried out from May 2005 to April 2007 involving 993 patients. VAP was defined based on clinical, radiological, and microbiological (&gt; 106 CFU/mL count in the tracheal aspirate) criteria. Four hundred and seventy four (47.7%) patients were submitted to mechanical ventilation with 141 (29.7%) VAPs, with S. aureus as the most frequent agent (41.2%). The phenotype ORSA accounted for 47.5% and OSSA for 52.5%,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ventilator-associated pneumonia; Hospital infection; Staphylococcus aureus; Resistant microorganisms.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600011

Central venous catheter-related bloodstream infection caused by Staphylococcus aureus: microbiology and risk factors
Provedor de dados: 55
Autores: Sadoyma,Geraldo; Diogo Filho,Augusto; Gontijo Filho,Paulo Pinto.
Although central vascular catheters (CVC) are indispensable in modern medicine, they are an important risk factor for primary bacteremias. We examined the incidence and risk factors associated with catheter-related bloodstream infection (CR-BSI) caused by Staphylococcus aureus in surgical patients. A prospective study was carried out in the Hospital das Clínicas da Universidade Federal de Uberlândia (HC-UFU) from September 2000 to December 2002. The skin insertion site, catheter tip, and blood were microbiologically analyzed. Demographics and risk factors were recorded for each patient, and cultures were identified phenotypically. Staphylococcus aureus was the most frequent pathogen, with an incidence rate of 4.9 episodes of CR-BSIs per 1,000...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Ventral venous catheter; Bloodstream infection.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000200006

Osteoarthritis in the neonate: risk factors and outcome
Provedor de dados: 55
Autores: Berberian,Griselda; Firpo,Verónica; Soto,Adriana; Lopez Mañan,Julio; Torroija,Cecilia; Castro,Graciela; Polanuer,Pablo; Espinola,Camilo; Piñeiro,José Luis; Rosanova,María Teresa.
OBJECTIVES: The aim of this study was to identify the clinical, radiological, and bacteriological features, risk factors, and outcome of neonates with bone and joint infections. STUDY DESIGN: Observational, retrospective, and analytical study of 77 patients less than 2 months of age, admitted to a tertiary neonatal intensive care unit (NICU) with the diagnosis of bone or joint infection, based on clinical, radiological, and microbiological criteria. RESULTS: Seventy-seven patients with 99 acute osteoarthritis foci in a 16 year period were included in the study. Risk factors for infection could be identified in 69% of the patients. The hip was the most frequent. Staphylococcus aureus was the main isolated microorganism. Twenty-nine infants (38%) had...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Osteoarthritis; Neonate; Staphylococcus aureus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400018

Success stories about severe pneumonia caused by Panton-Valentine leucocidin-producing Staphylococcus aureus
Provedor de dados: 55
Autores: Elisabeth,Paramythiotou; Maria,Souli; Irene,Galani; Helen,Giamarellou; Apostolos,Armaganidis.
We describe three cases of community-acquired necrotizing pneumonia which were caused by Panton-Valentine leucocidin-producing strains of Staphylococcus aureus (one of them methicillin sensitive). All cases were successfully treated without any sequelae for the patients due to the prompt initiation of adequate antimicrobial therapy. High suspicion toward this fatal pathogen was the key to the successful outcome of the patients.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Community-acquired pneumonia; Clindamycin; Staphylococcus aureus; Panton-Valentine leukocidin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300341

Phagocytosis and killing of epidemic methicillin-resistant Staphylococcus aureus by human neutrophils and monocytes
Provedor de dados: 55
Autores: Salgado,M.M.; Pignatari,A.C.C.; Bellinati-Pires,R.
Staphylococcus aureus is a pathogen that has been associated with nosocomial infections since the preantibiotic era. Since the introduction of antibiotics in medical practice in the 1940 s, methicillin-resistant S. aureus (MRSA) strains have been emerging in various parts of the world. In view of the important role of the phagocytic system in the defense against this bacteria, we decided to study phagocytosis by neutrophils and monocytes of an epidemic MRSA strain in São Paulo, Brazil, in comparison with methicillin-sensitive strains. Complement system opsonins are fundamental for efficient ingestion of the resistant and sensitive strains by both types of phagocytes. We found no association of the opsonic requirement of the MRSA strain with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Human phagocytes; Phagocytosis; Epidemic methicillin-resistant.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100005

Type IV SCCmec found in decade old Brazilian MRSA isolates
Provedor de dados: 55
Autores: Reinert,Cristina; McCulloch,John Anthony; Watanabe,Shinya; Ito,Teruyo; Hiramatsu,Keiichi; Mamizuka,Elsa Masae.
Methicillin-resistant Staphylococcus aureus (MRSA) commonly causes infection in hospitalized patients. Since its appearance in the 1960s, the SCCmec has evolved throughout the years into 5 different types (I-V), each bearing a different set of genes. Infection with MRSA SCCmec types I, II or III is almost exclusively restricted to hospitalised patients. However, recently, community acquired MRSA (CA-MRSA) infections have been reported with increasing frequency, usually caused by a type IV SCCmec MRSA in nosocomial settings. We studied the prevalence of SCCmec types in 50 nosocomial strains collected from 1995 to 1999. The SCCmec complex type and presence of Panton-Valentine leukocidin (PVL) were determined by PCR. Strains had been previously typed by PFGE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nosocomial infections; Type IV SCCmec; Staphylococcus aureus; Methicillin-resistant; Brazil.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300010

Staphylococcus aureus in tonsils of patients with recurrent tonsillitis: prevalence, susceptibility profile, and genotypic characterization
Provedor de dados: 55
Autores: Cavalcanti,Veraluce Paolini; Camargo,Leandro Azevedo de; Moura,Fabiano Santana; Melo Fernandes,Edson Júnior de; Lamaro-Cardoso,Juliana; Braga,Carla Afonso da Silva Bitencourt; André,Maria Cláudia Porfirio.
ABSTRACT  Introduction: Bacterial tonsillitis is an upper respiratory tract infection that occurs primarily in children and adolescents. Staphylococcus aureus is one of the most frequent pathogens in the etiology of tonsillitis and its relevance is due to its antimicrobial resistance and persistence in the internal tissues of the tonsils. Tonsillectomy is indicated in cases of recurrent tonsillitis after several failures of antibiotic therapy.  Material and methods: In this study we evaluated 123 surgically removed tonsils from patients who had history of recurrent tonsillitis. The tonsils were submitted to microbiological analysis for detection of S. aureus. The isolates were identified by PCR for femA gene. Antimicrobial susceptibility of the isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tonsillitis; Tonsillectomy; Staphylococcus aureus; Pulsed field gel electrophoresis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100008

The carriage of the serine-aspartate repeat protein-encodingsdr genes among Staphylococcus aureuslineages
Provedor de dados: 55
Autores: Liu,Huanle; Lv,Jingnan; Qi,Xiuqin; Ding,Yu; Li,Dan; Hu,Longhua; Wang,Liangxing; Yu,Fangyou.
ABSTRACTThe serine-aspartate repeat proteins (Sdr) are members of a family of surface proteins and contribute to the pathogenicity of Staphylococcus aureus. Among 288 S. aureus isolates including 158 and 130 associated with skin and soft tissue infections and bloodstream infection, respectively; 275 (95.5%) were positive for at least one of threesdr genes tested. The positivity rates for sdrC, sdrD, and sdrE among S. aureusisolates were 87.8% (253/288), 63.9% (184/288), and 68.1% (196/288), respectively. 224 (77.8%) of 288 isolates were concomitantly positive for two or three sdr genes. There was an association between carriage ofsdrE and methicillin-resistant S. aureus(MRSA) isolates, while the carriage rates of sdrC andsdrD in MRSA isolates were similar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Sdr genes; Clonal lineages.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500498

Modulation of drug resistance and biofilm formation of Staphylococcus aureus isolated from the oral cavity of Tunisian children
Provedor de dados: 55
Autores: Zmantar,Tarek; Ben Slama,Rihab; Fdhila,Kais; Kouidhi,Bochra; Bakhrouf,Amina; Chaieb,Kamel.
Abstract  Objectives: This study aims to investigate the antimicrobial and the anti-biofilm activities of Lactobacillus plantarum extract (LPE) against a panel of oral Staphylococcus aureus (n = 9) and S. aureus ATCC 25923. The in vitro ability of LPE to modulate bacterial resistance to tetracycline, benzalchonium chloride, and chlorhexidine were tested also.  Methods: The minimum inhibitory concentrations (MICs) and the minimal bactericidal concentrations of Lactobacillus plantarum extract, tetracycline, benzalchonium chloride and clohrhexidine were determined in absence and in presence of a sub-MIC doses of LPE (1/2 MIC). In addition, the LPE potential to inhibit biofilm formation was assessed by microtiter plate and atomic force microscopy assays....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibacterial; Staphylococcus aureus; Biofilm; Antibiotics; Synergetic; Lactobacillus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100027

Molecular identification and antimicrobial susceptibility of Staphylococcus aureus nasal isolates from medical students in Cartagena, Colombia
Provedor de dados: 55
Autores: Bettin,Alfonso; Causil,Ceyla; Reyes,Niradiz.
Staphylococcus aureus (SA) remains a major cause of nosocomial and community-acquired infections worldwide. Nasal carriage of this bacterium among hospital personnel constitutes an important source for nosocomial infections. A cross-sectional study enrolling the whole medical student population (n = 387) of the School of Medicine at the Universidad de Cartagena, Colombia, was conducted to evaluate the carriage rates of both methicillin sensitive-and methicillin resistant-SA, the frequency of Panton-Valentine leukocidin genes in the isolates, and risk factors associated with carriage in this selected population. After signing an informed consent, participants completed a survey related to possible risk factors for colonization, and nasal swabs were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carrier state; Staphylococcus aureus; Methicillin resistance; Panton-Valentine leukocidin.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000400003

Molecular techniques for MRSA typing: current issues and perspectives
Provedor de dados: 55
Autores: Trindade,P. A.; McCulloch,J. A.; Oliveira,G. A.; Mamizuka,E. M.
Staphylococcus aureus has long been recognised as an important pathogen in human disease. Serious staphylococcal infections can frequently occur in inpatients and may lead to dire consequences, especially as to therapy with antimicrobial agents. The increase in the frequency of Methicillin-Resistant Staphylococcus aureus (MRSA) as the causal agent of nosocomial infection and the possibility of emergence of resistance to vancomycin demands a quick and trustworthy characterization of isolates and identification of clonal spread within hospitals. Enough information must be generated to permit the implementation of appropriate measures for control of infection, so that outbreaks can be contained. Molecular typing techniques reviewed in this manuscript include:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Molecular epidemiology; Genotyping; Methicillin resistance.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000100005

Isolation, pathogenicity and disinfection of Staphylococcus aureus carried by insects in two public hospitals of Vitória da Conquista, Bahia, Brazil
Provedor de dados: 55
Autores: Oliveira,Pollianna S.; Souza,Simone G.; Campos,Guilherme B.; Silva,Danilo C.C. da; Sousa,Daniel S.; Araújo,Suerda P.F.; Ferreira,Laiziane P.; Santos,Verena M.; Amorim,Aline T.; Santos,Angelita M.O.G.; Timenetsky,Jorge; Cruz,Mariluze P.; Yatsuda,Regiane; Marques,Lucas M.
Currently, hospital infection is a serious public health problem, and several factors may influence the occurrence of these infections, including the presence of insects, which are carriers of multidrug-resistant bacterial species. The aim of this study was to isolate staphylococci carried by insects in two public hospitals of Vitoria da Conquista, Bahia and to identify the resistance profile, pathogenicity and efficacy of disinfection of the premises. A total of 91 insects were collected in 21 strategic points of these hospitals, and 32 isolated strains ofStaphylococcus aureus were isolated. Based on antibiogram and Minimum Inhibitory Concentration results, 95% of these strains were susceptible to oxacillin. These strains were also evaluated for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insects; Staphylococcus aureus; Resistance; Virulence factors; Disinfection.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000200129

Daptomycin to bone and joint infections and prosthesis joint infections: a systematic review
Provedor de dados: 55
Autores: Telles,João Paulo; Cieslinski,Juliette; Tuon,Felipe Francisco.
ABSTRACT  Backgroud: Daptomycin has been used in bone and joint infections (BJI) and prosthesis joint infections (PJI) considering spectrum of activity and biofilm penetration. However, the current experience is based on case reports, case series, cohorts, and international surveys. The aim of this systematic review was to evaluate studies about daptomycin treatment efficacy in BJI/PJI compared to other antibiotic regimens.  Methods: PubMed, LILACS, Scielo and Web of Science databases were searched for articles about daptomycin and treatment of BJI and PJI from inception to March 2018. Inclusion criteria were any published researches that included patients with BJI treated with daptomycin. Diagnosis of BJI was based on clinical, laboratory and radiological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Daptomycin; Staphylococcus aureus; Osteomyelitis; Joint infection; Arthritis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300191

Lessons to learn with the methicillin-resistant Staphylococcus aureus control in Norway
Provedor de dados: 55
Autores: Cruz,Elaine Drehmer de Almeida; Pimenta,Fabiana Cristina; Andersen,Bjorg Marit; Gir,Elucir.
The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Norway is low, compared to other European and American countries. The health system includes mandatory case reporting and has written guidelines for prevention and control. This communication describes the national public policies related to MRSA obtained from documents and academic experience gained during a doctoral fellowship in Oslo, Norway. The painstaking procedures used for investigating suspected cases, including health professionals, decolonization and case monitoring, could be important tools to be used by countries with a high prevalence of MRSA.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Staphylococcus aureus; Resistance to methicillin; Hospital infection epidemiological surveillance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600015

Staphylococcus aureus bacteremia: what is the impact of oxacillin resistance on mortality?
Provedor de dados: 55
Autores: Cassettari,V.C.; Strabelli,T.; Medeiros,E.A.S.
In order to analyse the impact of oxacillin resistance on the mortality of Staphylococcus aureus bacteremia, and to assess the antimicrobial susceptibility of community-acquired strains in two large university hospitals (the Instituto do Coração do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo and the Instituto Central do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo), we carried out a four-month-long prospective cohort study, which included 163 consecutive cases of S. aureus bacteremia. Of these, 140 (85.9%) were hospital-acquired, 9 (5.5%) were community-acquired and 14 (8.6%) were of indeterminate origin. No cases of community-acquired infection by oxacillin-resistant S. aureus was identified....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Bacteremia; Mortality.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100012

In vitro antimicrobial activity of the aminoglycoside arbekacin tested against oxacillin-resistant Staphylococcus aureus isolated in Brazilian hospitals
Provedor de dados: 55
Autores: Cordeiro,Julio C. R.; Reis,Adriana O.; Miranda,Eliete A.; Sader,Helio S.
Arbekacin is an aminoglycoside used in Japan for treating infections caused by gentamicin and oxacillin-resistant S. aureus (ORSA). The objective of this study was to determine the in vitro antimicrobial activity of arbekacin against 454 clinical isolates of ORSA. The isolates were consecutively collected between January and July, 2000, from patients hospitalized in 8 Brazilian medical centers. The antimicrobial susceptibility testing was performed by disk diffusion method according to NCCLS recommendations. The vast majority of the isolates, 453 strains (99.8%), were considered susceptible to arbekacin based on the criteria proposed by the Requirements for Antibiotic Products of Japan. Only 1 isolate (0.2%) was classified as resistant. On the other hand,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Arbekacin; Oxacillin-resistant; ORSA; Antimicrobial resistance; Nosocomial Infections.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000300005

Epidemiology of Staphylococcus aureus infections and nasal carriage at the Ibn Rochd University Hospital Center, Casablanca, Morocco
Provedor de dados: 55
Autores: Zriouil,Sanaâ Bouhali; Bekkali,Mohammed; Zerouali,Khalid.
Infections caused by Staphylococcus aureus are a major problem in hospitals. The multidrug resistance and the nasal carriage of S. aureus play a key role in the epidemic of these infections. In this prospective study, 160 S. aureus strains were isolated from pathological samples of patients (79 cases) and nasal swabs (81) of cases and controls from January to July 2007. The susceptibility to 16 antibiotics, including cefoxitin, was determined by the agar diffusion method, and methicillin resistance was confirmed by amplifying the mecA gene by polymerase chain reaction (PCR). The prevalence of methicilin-resistant S. aureus (MRSA) was high in the burns (57.7%) and dermatology (39.4%) wards, and the MRSA strains isolated were extremely multi-resistant, but...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Drug resistance; Methicillin-resistant; Staphylococcus aureus; Nasal cavity; Staphylococcal infections.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300011

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from a Brazilian university hospital
Provedor de dados: 55
Autores: Martins,André; Riboli,Danilo Flávio Moraes; Pereira,Valéria Cataneli; Cunha,Maria de Lourdes Ribeiro de Souza da.
The aim of this study was to perform SCCmec typing in Staphylococcus aureus isolates and to characterize the clonal profile of these isolates. Forty-six mecA gene-positive strains isolated between 2002 and 2006 were submitted to antimicrobial resistance testing by the E-test, SCCmec typing by multiplex PCR, and clonal profile analysis by pulsed-field gel electrophoresis. Forty-one (89.1%) isolates were typed as SCCmec III and five (10.9%) as SCCmec IV. Four circulating clones were detected, one of them comprising isolates related to the Brazilian epidemic clone. This clone was detected throughout the study period. The SCCmec III isolates were associated with a high rate of multidrug resistance and clonal dissemination of methicillin-resistant S. aureus in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Staphylococcus aureus; MRSA; SCCmec; BEC.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300331

Staphylococcus aureus bacteremia: comparison of two periods and a predictive model of mortality
Provedor de dados: 55
Autores: Conterno,Lucieni de Oliveira; Wey,Sérgio Barsanti; Castelo,Adauto.
Staphylococcus aureus is an important pathogen causing bacteremia, primarily affecting hospitalized patients. We studied the epidemiology of S. aureus bacteremia, comparing two periods (early and mid 1990s) and developed a predictive model of mortality. A nested case-control was done. All 251 patients over 14 years old with positive blood cultures for S. aureus were selected. MRSA (methicillin resistant S. aureus) was isolated in 63% of the cases. When comparing the two periods MRSA community-acquired bacteremia increased from 4% to 16% (p=0.01). There was no significant difference in the mortality rate between the two periods (39% and 33%, p=0.40). Intravascular catheters provoked 24% of the cases of bacteremia and were associated with the lowest rate of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Bacteremia; Mortality.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000600004

Brazilian experience in EU-CORE: daptomycin registry and treatment of serious Gram-positive infections
Provedor de dados: 55
Autores: Timerman,Artur; Brites,Carlos; Bicudo,Eliana; Grinbaum,Renato S.; Costa Filho,Rubens; Carrilho,Claudia D.M.; Bichels,Andre; Barreto,Tânia.
OBJECTIVES: To collect data about non-controlled prescribing use of daptomycin and its impact among Brazilian patients with serious Gram positive bacterial infection, as well as the efficacy and safety outcomes. MATERIALS AND METHODS: This is a multi-center, retrospective, non-interventional registry (August 01, 2009 to June 30, 2011) to collect data on 120 patients (44 patients in the first year and 76 patients in the second year) who had received at least one dose of commercial daptomycin in Brazil for the treatment of serious Gram-positive bacterial infection. RESULTS: Right-sided endocarditis (15.8%), complicated skin and soft tissue infections (cSSTI)wound (15.0%) and bacteremia-catheter-related (14.2%) were the most frequent primary infections; lung...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Daptomycin; Methicillin-resistant; Staphylococcus aureus; Bacteremia; Endocarditis; Skin diseases; Infectious.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600004

Prevalence of Staphylococcus aureus introduced into intensive care units of a University Hospital
Provedor de dados: 55
Autores: Cavalcanti,Silvana M.M.; França,Emmanuel R. de; Cabral,Carlos; Vilela,Marinalda A.; Montenegro,Francisco; Menezes,Daniela; Medeiros,Ângela C.R.
Staphylococcus aureus is one of the principal human pathogens that colonize healthy individuals in the community in general, and it is responsible for severe infections in hospitalized patients. Due to an increase in the prevalence of strains of methicillin-resistant S. aureus (MRSA), combating these microorganisms has become increasingly difficult. A descriptive study was carried out on 231 patients in intensive care at the Oswaldo Cruz University Hospital (HUOC) in Recife, Brazil between January and April 2003 to determine the prevalence of S. aureus and MRSA and to evaluate risk factors for colonization by these bacteria when introduced into Intensive Care Units (ICUs). Body secretions were collected from the nostrils, axillary and perineal regions, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Methicillin-resistant; Intensive care unit.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100010

Prevalence of phenotypic resistance of Staphylococcus aureus isolates to macrolide, lincosamide, streptogramin B, ketolid and linezolid antibiotics in Turkey
Provedor de dados: 55
Autores: Adaleti,Riza; Nakipoglu,Yasar; Ceran,Nurgul; Tasdemir,Cihan; Kaya,Fatma; Tasdemir,Semiha.
The incidence of drug-resistant pathogens differs greatly between countries according to differences in the usage of antibiotics. The purpose of this study was to investigate the phenotypic resistance of 321 methicillin resistance Staphylococcus aureus (MRSA) and 195 methicillin susceptible S. aureus (MSSA) in a total of 516 S. aureus strains to macrolide, lincosamide, streptogramin B (MLS B), ketolid, and linezolid. Disk diffusion method was applied to determine MLS B phenotype and susceptibility to different antibiotic agents. It was found that 54.6% of the isolates were resistant to erythromycin (ERSA), 48% to clindamycin, 55% to azithromycin, 58.7% to spiramycin, 34.7% to telithromycin, and 0.4% to quinupristin-dalfopristin, respectively. No strain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Macrolide; Lincosamide; Streptogramin B; Ketolid; Linezolid; MLS B.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100003

Primary pyomyositis and disseminated septic pulmonary emboli: a reactivated staphylococcal infection?
Provedor de dados: 55
Autores: Savvidou,Savvoula; Kalogiannis,Emmanouil; Tsakiri,Kalliopi; Gavra,Maria; Tsona,Afroditi.
Staphylococcal pyomyositis is a severe invasive soft tissue infection with high mortality rate that is increasingly being recognized even in temperate climates. In most cases predisposing factors are identified that include either source of skin penetration or/and impaired host immunocompetence. A case of primary, community-acquired pyomyositis of the left iliopsoas muscle in a 59-year-old immunecompetent woman, which was complicated with septic pulmonary emboli within 24 h after hospital admission, is presented. The patient was subjected to abscess drainage under computed tomography guidance. Both pus aspiration and blood cultures revealed methicillin-susceptible Staphylococcus aureus. Given the absolute absence of predisposing factors and a remote...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Pyomyositis; Staphylococcus aureus; Septic pulmonary emboli; Reactivation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000400457

Individual variation is the key to the development of a vaccine against Staphylococcus aureus: a comparative study between mice lineages
Provedor de dados: 56
Autores: dos Santos,D.P.; Muniz,I.P.R.; Queiroz,A.F.; Pereira,I.S.; Souza,M.P.A.; Lima,L.J.; Sousa,L.R.O.; Ribeiro,I.S.; Galantini,M.P.L.; Marques,L.M.; Figueiredo,T.B.; da Silva,R.A.A.
Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. aureus infection in intradermally immunized mice. C57BL/6 and A/j mice were immunized intradermally with S. aureus inactivated by heat and then challenged with viable strains in an air pouch model. At 6, 12, and 24 h after the challenge, euthanasia was performed, and the cellular profile of the inflammatory infiltrate, cytokines, and the bacterial load were evaluated in the air pouch lavages. Immunized mice demonstrated that the intradermal immunization with S. aureus promoted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunization; Staphylococcus aureus; Mice; Air pouch; Antibodies.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500616

Inactivation of Staphylococcus aureus and Salmonella enteritidis in tryptic soy broth and caviar samples by high pressure processing
Provedor de dados: 56
Autores: Fioretto,F.; Cruz,C.; Largeteau,A.; Sarli,T.A.; Demazeau,G.; El Moueffak,A.
We studied the action of high pressure processing on the inactivation of two foodborne pathogens, Staphylococcus aureus ATCC 6538 and Salmonella enteritidis ATCC 13076, suspended in a culture medium and inoculated into caviar samples. The baroresistance of the two pathogens in a tryptic soy broth suspension at a concentration of 10(8)-10(9) colony-forming units/ml was tested for continuous and cycled pressurization in the 150- to 550-MPa range and for 15-min treatments at room temperature. The increase of cycle number permitted the reduction of the pressure level able to totally inactivate both microorganisms in the tryptic soy broth suspension, whereas the effect of different procedure times on complete inactivation of the microorganisms inoculated into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High pressure processing; Staphylococcus aureus; Salmonella enteritidis; Tryptic soy broth; Caviar; Pressure cycles.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800015

PCR-based ribotyping of Staphylococcus aureus
Provedor de dados: 56
Autores: Oliveira,A.M.; Ramos,M.C.
Genotyping techniques are valuable tools for the epidemiologic study of Staphylococcus aureus infections in the hospital setting. Pulsed-field gel electrophoresis (PFGE) is the current method of choice for S. aureus strain typing. However, the method is laborious and requires expensive equipment. In the present study, we evaluated the natural polymorphism of the genomic 16S-23S rRNA region for genotyping purpose, by PCR-based ribotyping. Three primer pairs were tested to determine the size of amplicons produced and to obtain better discrimination with agar gel electrophoresis and ethidium bromide staining. The resolution of the typing system was determined using sets of bacteria obtained from clinical specimens from a large tertiary care hospital. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; PCR ribotyping; Genotyping; Methicillin-resistant Staphylococcus aureus.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200005

High prevalence of methicillin resistance and PVL genes among Staphylococcus aureus isolates from the nares and skin lesions of pediatric patients with atopic dermatitis
Provedor de dados: 56
Autores: Cavalcante,F.S.; Abad,E.D.; Lyra,Y.C.; Saintive,S.B.; Ribeiro,M.; Ferreira,D.C.; Santos,K.R.N. dos.
Staphylococcus aureus is highly prevalent among patients with atopic dermatitis (AD), and this pathogen may trigger and aggravate AD lesions. The aim of this study was to determine the prevalence of S. aureus in the nares of pediatric subjects and verify the phenotypic and molecular characteristics of the isolates in pediatric patients with AD. Isolates were tested for antimicrobial susceptibility, SCCmec typing, and Panton-Valentine Leukocidin (PVL) genes. Lineages were determined by pulsed-field gel electrophoresis and multilocus sequence typing (MLST). AD severity was assessed with the Scoring Atopic Dermatitis (SCORAD) index. Among 106 patients, 90 (85%) presented S. aureus isolates in their nares, and 8 also presented the pathogen in their skin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atopic dermatitis; Staphylococcus aureus; Nasal colonization; Skin lesions; SCORAD.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700588

Effectiveness of preoperative decolonization with nasal povidone iodine in Chinese patients undergoing elective orthopedic surgery: a prospective cross-sectional study
Provedor de dados: 56
Autores: Peng,H-M.; Wang,L-C.; Zhai,J-L.; Weng,X-S.; Feng,B.; Wang,W.
Staphylococcus aureus colonization in the nares of patients undergoing elective orthopedic surgery increases the potential risk of surgical site infections. Methicillin-resistant S. aureus (MRSA) has gained recognition as a pathogen that is no longer only just a hospital-acquired pathogen. Patients positive for MRSA are associated with higher rates of morbidity and mortality following infection. MRSA is commonly found in the nares, and methicillin-sensitive S. aureus (MSSA) is even more prevalent. Recently, studies have determined that screening for this pathogen prior to surgery and diminishing staphylococcal infections at the surgical site will dramatically reduce surgical site infections. A nasal mupirocin treatment is shown to significantly reduce the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nasal methicillin-resistant; Staphylococcus aureus; Orthopedic surgery; Nasal povidone iodine; Prospective cross-sectional study.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200615

Optimization of the RNeasy Mini Kit to obtain high-quality total RNA from sessile cells of Staphylococcus aureus
Provedor de dados: 56
Autores: Beltrame,C. O.; Côrtes,M. F.; Bandeira,P. T.; Figueiredo,A. M. S.
Biofilm formed by Staphylococcus aureus is considered an important virulence trait in the pathogenesis of infections associated with implantable medical devices. Gene expression analyses are important strategies for determining the mechanisms involved in production and regulation of biofilm. Obtaining intact RNA preparations is the first and most critical step for these studies. In this article, we describe an optimized protocol for obtaining total RNA from sessile cells of S. aureus using the RNeasy Mini Kit. This method essentially consists of a few steps, as follows: 1) addition of acetone-ethanol to sessile cells, 2) lysis with lysostaphin at 37°C/10 min, 3) vigorous mixing, 4) three cycles of freezing and thawing, and 5) purification of the lysate in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA preparation; MRSA; Biofilm; Sessile cells; Staphylococcus aureus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201071

Genotypic and phenotypic detection of capsular polysaccharides in Staphylococcus aureus isolated from bovine intramammary infections in Argentina
Provedor de dados: 58
Autores: Camussone,C.; Rejf,P.; Pujato,N.; Schwab,A.; Marcipar,I.; Calvinho,L.F.
Staphylococcus aureus (n=157) isolated from intramammary infections in Argentine dairy areas were evaluated for presence of cap5 and cap8 loci. Isolates carrying cap5 and cap8 were serotyped using specific antisera. Sixty four percent of the isolates were genotyped as cap5 or cap8 and 50% of them expressed CP5 or 8.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Capsular polysaccharides; Bovine mastitis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300023

Comparative analysis of agr groups and virulence genes among subclinical and clinical mastitis Staphylococcus aureus isolates from sheep flocks of the Northeast of Brazil
Provedor de dados: 58
Autores: Almeida,Lara M. de; Almeida,Mayra Zilta P.R.B. de; Mendonça,Carla L. de; Mamizuka,Elsa M.
Staphylococcus aureus is one of the most frequent mastitis causative agents in small ruminants. The expression of most virulence genes of S. aureus is controlled by an accessory gene regulator (agr)locus. This study aimed to ascertain the prevalence of the different agr groups and to evaluate the occurrence of encoding genes for cytotoxin, adhesins and toxins with superantigen activity in S. aureus isolates from milk of ewes with clinical and subclinical mastitis in sheep flocks raised for meat production The agr groups I and II were identified in both cases of clinical and subclinical mastitis. Neither the arg groups III and IV nor negative agr were found. The presence of cflA gene was identified in 100% of the isolates. The frequency of hla and lukE-D...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Agr groups; Virulence genes; Mastitis; Sheep flock.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200026

Biofilm formation by Staphylococcus aureus and Salmonella spp. under mono and dual-species conditions and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite
Provedor de dados: 58
Autores: Iñiguez-Moreno,Maricarmen; Gutiérrez-Lomelí,Melesio; Guerrero-Medina,Pedro Javier; Avila-Novoa,María Guadalupe.
Abstract The aim of this study was evaluated the biofilm formation by Staphylococcus aureus 4E and Salmonella spp. under mono and dual-species biofilms, onto stainless steel 316 (SS) and polypropylene B (PP), and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite. The biofilms were developed by immersion of the surfaces in TSB by 10 d at 37 °C. The results showed that in monospecies biofilms the type of surface not affected the cellular density (p &gt; 0.05). However, in dual-species biofilms on PP the adhesion of Salmonella spp. was favored, 7.61 ± 0.13 Log10 CFU/cm2, compared with monospecies biofilms onto the same surface, 5.91 ± 0.44 Log10 CFU/cm2 (p &lt; 0.05). The mono and dual-species biofilms were subjected to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; Salmonella spp.; Staphylococcus aureus; Disinfectants; Sensitivity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200310

Biochemical characteristics of typical and atypical Staphylococcus aureus in mastitic milk and environmental samples of Brazilian dairy farms
Provedor de dados: 58
Autores: Silva,Wladimir Padilha da; Destro,Maria Teresa; Landgraf,Mariza; Franco,Bernadette D.G.M.
This study reports results on the biochemical characteristics of 274 Staphylococcus aureus strains isolated from mastitic milk, hands of milkmen and milking machines. Features included colony morphology on Baird Parker agar (BPA), catalase, coagulase and thermonuclease tests. API-Staph (bioMérieux, France) was used as reference identification system. 39.4% of the colonies on BPA identified as S.aureus were atypical. They were more frequent in milk samples (94.4%). All S.aureus strains were coagulase positive but intensity of the test varied according to the source: environmental strains were 3+ or 4+, while 17.1% of strains isolated from milk presented only 1+ or 2+ results. The majority of atypical S.aureus strains presented a 4+ coagulase result. 2.2% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Atypical S.aureus; Coagulase; Thermonuclease; API-Staph; Baird-Parker agar; Milk; Mastitis; Environmental samples.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200008

Detection of Staphylococcus aureus with an intermediate profile to vancomycin (VISA) isolate from Santa Maria, RS
Provedor de dados: 58
Autores: Marques,Jaciane Baggiotto; Dalmolin,Tanise Vendruscolo; Bonez,Pauline Cordenonsi; Agertt,Vanessa Albertina; Campos,Marli Matiko Anraku de; Santos,Roberto Christ Vianna.
Twenty-three isolates of Staphylococcus aureus resistant to methicillin have been analyzed, being found a clinical isolate of VISA through microdilution technique. The others techniques were unable to detect such isolates. This is the first study that shows the presence of VISA in clinical isolates in the city of Santa Maria-RS.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Staphylococcus aureus; Vancomycin; Microdilution.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100040

Prior oropharyngeal colonization and ventilator-associated pneumonia
Provedor de dados: 58
Autores: Moreira,Michel Rodrigues; Ferreira,Joseane Cristina; Darini,Ana Lúcia da Costa; Gontijo Filho,Paulo Pinto.
This study evaluated the relationship between previous colonization of the oropharynx and development of ventilator-associated pneumonia through the classification of genomic fingerprint pattern by pulsed-field gel electrophoresis of both oxacillin-resistant and oxacillin-susceptible Staphylococcus aureus isolates obtained from hospitalized patients in an intensive care unit.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oropharyngeal colonization; Ventilator-associated pneumonia; Staphylococcus aureus.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300015

Formation of biofilms by Staphylococcus aureus on stainless steel and glass surfaces and its resistance to some selected chemical sanitizers
Provedor de dados: 58
Autores: Marques,Simone Cristina; Rezende,Jaíne das Graças Oliveira Silva; Alves,Lizandra Aparecida de Freitas; Silva,Belami Cássia; Alves,Eduardo; Abreu,Luiz Ronaldo de; Piccoli,Roberta Hilsdorf.
The objectives of this work were to verify the capability of Staphylococcus aureus of forming bio-film on stainless steel and glass surfaces; to evaluate the efficiency of sodium dichloroisocyanurate, hydrogen peroxide and peracetic acid in inactivating Staphylococcus aureus cells adhered onto these surfaces; and to visualize biofilm development by scanning electron microscopy before and after sanitizer treatment. The surfaces studied consisted of 10x20mm chips immersed in Petri dishes containing BHI broth inoculated with S. aureus ATCC 25923. Biofilm formation was observed after 15-day incubation, when the cells were removed using the swab technique, followed by Baird Parker agar plating. Also, the efficiency of the chemical sanitizers on the chip...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilm; Stainless steel; Glass; Staphylococcus aureus; Sanitizers; Scanning electron microscopy.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300029

Biofilm formation by Staphylococcus aureus from food contact surfaces in a meat-based broth and sensitivity to sanitizers
Provedor de dados: 58
Autores: Souza,Evandro Leite de; Meira,Quênia Gramile Silva; Barbosa,Isabella de Medeiros; Athayde,Ana Júlia Alves Aguiar; Conceição,Maria Lúcia da; Siqueira Júnior,José Pinto de.
This study assessed the capacity of adhesion, the detachment kinetic and the biofilm formation by Staphylococcus aureus isolated from food services on stainless steel and polypropylene surfaces (2 x 2 cm) when cultivated in a meat-based broth at 28 and 7 ºC. It was also to study the efficacy of the sanitizers sodium hypochlorite (250 mg/L) and peracetic acid (30 mg/L) in inactivating the bacterial cells in the preformed biofilm. S. aureus strains adhered in high numbers regardless the assayed surface kind and incubation temperature over 72 h. Cells detachment of surfaces revealed high persistence over the incubation period. Number of cells needed for biofilm formation was noted at all experimental systems already after 3 days. Peracetic acid and sodium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Food processing environment; Cross-contamination; Biofilm; Sanitizers.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100010

Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infections
Provedor de dados: 58
Autores: Bonesso,Mariana Fávero; Marques,Silvio Alencar; Camargo,Carlos Henrique; Fortaleza,Carlos Magno Castelo Branco; Cunha,Maria de Lourdes Ribeiro de Souza da.
The aim of this study was to determine the prevalence of Staphylococcus aureus and risk factors for the acquisition of MRSA (Methicillin Resistant Staphylococcus aureus) as the main cause of skin and soft tissue infections. S. aureus were characterized for the presence of PVL, TSST-1 and mecA genes. SCCmec typing was carried out in mecA positive strains and PFGE was performed only in these strains. During the study period, 127 outpatients attending a dermatology clinical the Botucatu Medical School, a regional tertiary hospital in Botucatu, Sao Paulo, Brazil, were diagnosed with active skin infections. A total 66 (56.9%) S. aureus strains were isolated. The methicillin resistance gene mecA was detected in seven (10.6%) S. aureus strains. The SCCmec types...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Resistance; Skin infections; Virulence; Epidemiology.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400034

Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques
Provedor de dados: 58
Autores: Vieira-da-Motta,Olney; Folly,Márcio Manhães; Sakyiama,Cássia Camargo Hagen.
Contamination of fresh milk with Staphylococcus aureus was assessed comparatively through routine phenotypic (coagulase tube test and coagulase slide test) and genotypic (PCR) screening of 128 S. aureus strains isolated from 555 milk samples. These samples were collected from 362 cows with subclinical mastitis, hosted in different dairy herds at various locations of the Northern and Northeastern rural areas of the State of Rio de Janeiro, 39.7% of which were CMT-positive. All S. aureus isolates tested positive for the presence of the coagulase gene by PCR and the isolates could be grouped into four distinct classes according to the size of the PCR product. The strains also yielded variable results when assayed with coagulase test. Taken together, these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine mastitis; Staphylococcus aureus; Coagulase; PCR.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100007

Action of peracetic acid on Escherichia coli and Staphylococcus aureus in suspension or settled on stainless steel surfaces
Provedor de dados: 58
Autores: Kunigk,Leo; Almeida,Maria C.B.
The efficiency of a commercial peracetic acid sanitizer on destruction of Staphylococcus aureus and Escherichia coli was evaluated using two distinct methods. The first method is the AOAC suspension test and the second is a method proposed by one of the authors in which the microbial cells are settled on a stainless steel surface and then treated with the sanitizer. The results showed that when in suspension S. aureus was more resistant to the sanitizer than E. coli. When S. aureus was settled on the stainless steel surface, the contact time between the sanitizer and the microorganisms to attain a 6.5 log reduction in the number of viable cells was three times greater than when the cells were in suspension.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peracetic acid; Staphylococcus aureus; Escherichia coli; Stainless steel surface; Sanitizers.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100009

OCCURRENCY OF MALASSEZIA PACHYDERMATIS AND OTHER INFECTIOUS AGENTS AS CAUSE OF EXTERNAL OTITIS IN DOGS FROM RIO GRANDE DO SUL STATE, BRAZIL (1996/1997)
Provedor de dados: 58
Autores: Nobre,Márcia de Oliveira; Castro,Ângela Pötter de; Nascente,Patrícia da Silva; Ferreiro,Laerte; Meireles,Mario Carlos A.
The purpose of this work was to evaluate the frequency of Malassezia pachydermatis infection and other infectious agents in dogs with external otitis and with healthy auditory tubes. Clinical manifestations of external otitis were evaluated. Samples from the auditory tube of 102 dogs with otitis and from 32 healthy dogs were submitted to direct microscopic examination and cultured in blood agar and Sabouraud dextrose agar with chloramphenicol and cycloheximide. Direct examination showed more than ten cells of M. pachydermatis in 52.0% of the samples from dogs with otitis, but in only 21.8% of the healthy auditory tube samples. M. pachydermatis was isolated in 37.5% of the samples from dogs with healthy auditory tube and 76.5% (p&lt;0.01) of the samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malassezia pachydermatis; Staphylococcus aureus; Otitis; Dog.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300017

Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis
Provedor de dados: 58
Autores: Ciftci,Alper; Findik,Arzu; Onuk,Ertan Emek; Savasan,Serap.
This study aimed to detect methicillin resistant and slime producing Staphylococcus aureus in cases of bovine mastitis. A triplex PCR was optimized targetting 16S rRNA, nuc and mecA genes for detection of Staphylococcus species, S. aureus and methicillin resistance, respectively. Furthermore, for detection of slime producing strains, a PCR assay targetting icaA and icaD genes was performed. In this study, 59 strains were detected as S. aureus by both conventional tests and PCR, and 13 of them were found to be methicillin resistant and 4 (30.7%) were positive for mecA gene. Although 22 of 59 (37.2%) S. aureus isolates were slime-producing in Congo Red Agar, in PCR analysis only 15 were positive for both icaA and icaD genes. Sixteen and 38 out of 59 strains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; IcaA; IcaD; Slime; Mastitis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200009

Assessing the epidemiological data of Staphylococcus aureus food poisoning occurred in the State of Rio Grande do Sul, Southern Brazil
Provedor de dados: 58
Autores: Lima,Gustavo Costalunga; Loiko,Márcia Regina; Casarin,Letícia Sopeña; Tondo,Eduardo Cesar.
Staphylococcal food poisoning is one of the most frequent foodborne illnesses worldwide and it is caused by the ingestion of food contaminated with enterotoxins produced by some strains of Staphylococcus (S.) aureus. In the State of Rio Grande do Sul (RS), Southern Brazil, S. aureus has been identified as the second most frequent agent of foodborne illnesses in the last two decades. The aim of the present study was to assess and analyse the epidemiological data of S. aureus food poisoning occurred in the State of RS during the years of 2000 to 2002. The official records of epidemiological investigations carried out by the Sanitary Surveillance Services of the State of RS were analysed. Among foodborne outbreaks for which aetiology was determined, S. aureus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Food poisoning; RS State; Brazil.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300014

Antimicrobial activity of Aspilia latissima (Asteraceae)
Provedor de dados: 58
Autores: Souza,Jeana M.E.; Chang,Marilene R.; Brito,Daniela Z.; Farias,Katyuce S.; Damasceno-Junior,Geraldo A.; Turatti,Izabel C.C.; Lopes,Norberto P.; Santos,Edson A.; Carollo,Carlos A.
Abstract We evaluated the antimicrobial activity of Aspilia latissima - an abundant plant from the Brazilian Pantanal region - against Candida albicans, Candida parapsilosis, Candida krusei, Candida tropicalis, Pseudomonas aeruginosa, Enterococcus faecalis, Escherichia coli and Staphylococcus aureus. The crude extracts and fractions showed activity in all tested microorganisms. The chloroform fraction of the leaves and roots showed the most antimicrobial activity against S. aureus, with an MIC of 500 μg/mL. This fraction was submitted to bioautographic assays to characterize the activity of the compounds. Two bands from the leaves (L-A and L-B) and three bands from the roots (R-C, R-D and R-E) were bioactive. Within the root-derived bands, the terpene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspilia latissima; Staphylococcus aureus; Bioautographic; MIC; Terpenes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401103

Occurrence of Staphylococcus aureus in raw milk produced in dairy farms in São Paulo state, Brazil
Provedor de dados: 58
Autores: Fagundes,Helena; Barchesi,Luciana; Nader Filho,Antonio; Ferreira,Luciano Menezes; Oliveira,Carlos Augusto Fernandes.
The objective of the present study was to evaluate the occurrence of Staphylococcus aureus in milk produced in 37 farms located in the regions of Ribeirão Preto and São Carlos, state of São Paulo, Brazil. Two-hundred and eight samples of milk from individual cows showing subclinical mastitis, and 37 samples of bulk tank milk were analyzed. S. aureus strains were detected in 18 (7.3%) milk samples: 14 (6.7%) from samples of individual cows, and 4 (10.8%) from bulk tank milk. Two individual milk samples (14.3%) and two bulk milk samples contained enterotoxigenic S. aureus. PFGE analysis revealed the genetic heterogeneity of the strains isolated from raw milk, which presented to 13 S. aureus patterns. Results confirmed the potential transmission of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Raw milk; Bovine; Mastitis; Enterotoxin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200018

Enterotoxigenicity of Staphylococcus aureus isolated from traditional and commercial dairy products marketed in Iran
Provedor de dados: 58
Autores: Rahimi,Ebrahim.
This study was conducted to determine the prevalence rate, enterotoxigenecity, and antimicrobial resistance of S. aureus isolated from dairy products in Iran. From September 2010 to July 2011, a total of 347 samples from various dairy products, traditional and commercial, were collected from randomly selected retail stores. Overall, 20 samples (5.8%) were found to be contaminated with S. aureus. The highest prevalence of S. aureus was found in traditional cheese (11.1%), followed by traditional ice-cream (5.9%), cream (5.6%), and butter (5.3%). The ability to synthesize classical staphylococcal enterotoxins (SEA-E) was determined in 7 of 20 (35%) isolates by using ELISA. SE type C was the most common enterotoxin found in the isolated S. aureus (42.9%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial resistance; Dairy products; SE genes; Staphylococcal enterotoxins; Staphylococcus aureus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200008

Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
Provedor de dados: 58
Autores: Martins,Katheryne Benini; Faccioli-Martins,Patricia Yoshida; Riboli,Danilo Flávio Moraes; Pereira,Valéria Cataneli; Fernandes,Simone; Oliveira,Aline A.; Dantas,Ariane; Zafalon,Luiz Francisco; Cunha,Maria de Lourdes Ribeiro de Souza da.
The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Sheep; Staphylococcus aureus; Antibiotic resistance; Virulence factors.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535

Conjunctival microbial flora of clinically normal persons who work in a hospital environment
Provedor de dados: 58
Autores: Trindade,Rita de Cássia; Bonfim,Ana Cristina Rocha; Resende,Maria Aparecida.
The objective of the work was to study the microbiota of the conjunctival secretion of health professionals. Samples were collected from the clinically normal eyes of 40 health professionals in four different sectors, Proctology, General Intensive Care Unit (ICU), Male Ward, and Oncology of Hospital das Clínicas Dr. Augusto Leite, Aracaju, SE, Brazil. Ten professionals from each sector were selected. The samples were inoculated into various culture media: blood agar, Chapman agar, EMB medium (Teague), and Sabouraud dextrose agar with chloramphenicol. The bacteria most frequently isolated from all the sectors were Staphylococcus epidermidis (45.0%) and Bacillus sp (29.0%). The least frequent bacteria were Proteus sp (6.1%), Staphylococcus aureus (4.1%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conjunctival microbiota; Hospital environment; Staphylococcus epidermidis; Staphylococcus aureus.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100004

Carriage frequency, phenotypic and genotypic characteristics of Staphylococcus aureus isolated from dialysis and kidney tranplant patients at a hosptial in northern Paraná
Provedor de dados: 58
Autores: Giarola,Luciana Borges; Santos,Rosiane Ribeiro dos; Tognim,Maria Cristina Bronharo; Borelli,Sueli Donizete; Bedendo,João.
The objective of the present study was to determine the frequency of Staphylococcus aureus nasal carriage among dialysis and kidney transplant patients, to identify the antimicrobial resistance profile of these strains and to verify their genetic profiles with the RW3A primer. The study included 159 individuals, comprising 111 dialysis and 48 kidney transplant patients. Of the 48 transplant patients, 75% were positive for S. aureus, whereas 49% of the 111 dialysis patients were carriers. Two samples yielded conflicting results for oxacillin sensitivity between the disk diffusion and minimum inhibitory concentration (MIC) assays: both were sensitive by the disk diffusion assay and resistant by MIC (4 μg/ml). In the antibiogram by disk diffusion, ten samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Nasal carrier; Resistance; MecA gene; Genetic typing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300011

Quantification of biofilm production on polystyrene by Listeria, Escherichia coli and Staphylococcus aureus isolated from a poultry slaughterhouse
Provedor de dados: 58
Autores: Rodrigues,Laura Beatriz; Santos,Luciana Ruschel dos; Tagliari,Vinícius Zancanaro; Rizzo,Natalie Nadin; Trenhago,Graciela; Oliveira,Amauri Picollo de; Goetz,Franciane; Nascimento,Vladimir Pinheiro do.
This study assessed biofilm formation on polystyrene by Staphylococcus aureus, Listeria monocytogenes, L. welshimeri and Escherichia coli, isolated from a slaughtering plant, grown on tryptic soy broth (TSB) using different glucose concentrations. The tested bacteria produced biofilm in at least one of the concentrations used, and some of them were strong biofilm producers.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria; Escherichia coli; Staphylococcus aureus; Biofilm; Polystyrene.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400029

Ocurrence of Staphylococcus aureus and multiplex pcr detection of classic enterotoxin genes in cheese and meat products
Provedor de dados: 58
Autores: Pelisser,Marcia Regina; Klein,Cátia Silene; Ascoli,Kelen Regina; Zotti,Thaís Regina; Arisi,Ana Carolina Maisonnave.
Multiplex PCR was used to investigate the presence of enterotoxins genes (sea, seb, sec, sed and see) and femA gene (specific for Staphylococcus aureus) in coagulase-positive staphylococci (CPS) isolated from cheese and meat products. From 102 CPS isolates, 91 were positive for femA, 10 for sea, 12 for sed and four for see.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Enterotoxins; Multiplex PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100025

Evaluation of the in vitro antimicrobial activity of an ethanol extract of Brazilian classified propolis on strains of Staphylococcus aureus
Provedor de dados: 58
Autores: Pamplona-Zomenhan,Lucila Coelho; Pamplona,Beatriz Coelho; Silva,Cely Barreto da; Marcucci,Maria Cristina; Mimica,Lycia Mara Jenné.
Staphylococcus aureus (S. aureus) is one of the most frequent causes of hospital acquired infections. With the increase in multiple drug resistant strains, natural products such as propolis are a stratagem for new product discovery. The aims of this study were: to determine the in vitro antimicrobial activity of an ethanol extract of propolis; to define the MIC50 and MIC90 (Minimal Inhibitory Concentration - MIC) against 210 strains of S. aureus; to characterize a crude sample of propolis and the respective ethanol extract as to the presence of predetermined chemical markers. The agar dilution method was used to define the MIC and the high performance liquid chromatography (HPLC) method was used to characterize the samples of propolis. MIC results ranged...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Staphylococcus aureus; Antimicrobial Activity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400002

Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina
Provedor de dados: 58
Autores: Lucero Estrada,Cecilia S.M.; Alcaráz,Lucia E.; Satorres,Sara E.; Manfredi,Eduardo; Velázquez,Lidia del C.
An increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Enterotoxigenic strains; Fruit salads; Antimicrobial compounds; Multiplex PCR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400018

Molecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms
Provedor de dados: 58
Autores: Souza,Viviane; Nader Filho,Antonio; Melo,Poliana de Castro; Ferraudo,Guilherme Moraes; Ferraudo,Antônio Sérgio; Conde,Sandra de Oliveira; Fogaça Junior,Flavio Augusto.
The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples' collection sites with the highest isolation frequency of Staphylococcus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastitis; Staphylococcus aureus; Bulk tank; PCR; PFGE.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400050

Novel nonsense mutation in the katA gene of a catalase-negative Staphylococcus aureus strain
Provedor de dados: 58
Autores: Lagos,Jaime; Alarcón,Pedro; Benadof,Dona; Ulloa,Soledad; Fasce,Rodrigo; Tognarelli,Javier; Aguayo,Carolina; Araya,Pamela; Parra,Bárbara; Olivares,Berta; Hormazábal,Juan Carlos; Fernández,Jorge.
Abstract We report the first description of a rare catalase-negative strain of Staphylococcus aureus in Chile. This new variant was isolated from blood and synovial tissue samples of a pediatric patient. Sequencing analysis revealed that this catalase-negative strain is related to ST10 strain, which has earlier been described in relation to S. aureus carriers. Interestingly, sequence analysis of the catalase gene katA revealed presence of a novel nonsense mutation that causes premature translational truncation of the C-terminus of the enzyme leading to a loss of 222 amino acids. Our study suggests that loss of catalase activity in this rare catalase-negative Chilean strain is due to this novel nonsense mutation in the katA gene, which truncates the enzyme...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Catalase-negative; Catalase gene; KatA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100177

Effect of galbanic acid, a sesquiterpene coumarin from Ferula szowitsiana, as an inhibitor of efflux mechanism in resistant clinical isolates of Staphylococcus aureus
Provedor de dados: 58
Autores: Bazzaz,Bibi Sedigheh Fazly; Memariani,Zahra; Khashiarmanesh,Zahra; Iranshahi,Mehrdad; Naderinasab,Mahbobeh.
Galbanic acid, a sesquiterpene coumarin from Ferula szowitsiana roots, was investigated for its potentiating effect on the antimicrobial activity of antibiotics as well as ethidium bromide, in 6 multidrug resistance (MDR) clinical isolates of Staphylococcus aureus. Galbanic acid had inhibitory effect on none of the isolated bacteria tested (up to 800 µg /ml). The MIC range of ciprofloxacin, tetracycline and ethidium bromide, against all tested S. aureus were 10-80, 10-80 and 4-16 µg/ml, respectively. These were reduced to ≤2.5-5, 2.5-5 and 0.5-2 µg/ml in the presence of galbanic acid (300 µg /ml) or verapamil (100 µg /ml). The rate of ethidium bromide (2 µg /ml) accumulation in clinical isolates was enhanced with galbanic acid (300 µg /ml). There is also a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Efflux pump inhibitor; Ferula szowitsiana; Galbanic acid; Staphylococcus aureus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300006

Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis
Provedor de dados: 58
Autores: Melo,Poliana de Castro; Ferreira,Luciano Menezes; Nader Filho,Antônio; Zafalon,Luiz Francisco; Vicente,Hinig Isa Godoy; Souza,Viviane de.
Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; Bovine mastitis; Phenotypic and molecular analysis; Staphylococcus aureus.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100017

Bacteriological features of infectious spondylodiscitis at Mohammed V Military Teaching Hospital of Rabat
Provedor de dados: 58
Autores: Zohoun,A; Ngoh Akwa,E; El Ochi,M; Oragwu,N; Akhaddar,A; Albouzidi,A; El Ouennass,M.
To review the bacteriological features of infectious spondylodiscitis and provide recommendations for the initial therapy which remains empirical in our context. Retrospective study including patients diagnosed with spondylodiscitis over a period of 4 years (2006-2009) at the Rabat Military Teaching Hospital. During the study period, we analysed 30 cases: the mean age was 49.9 years and 21 cases (70%) were male. The patients were predominantly hospitalized in neurosurgery department (15/30) followed by rheumatology department (10/30). The site of infection was lumbar in 21 cases (21/30), dorsal in 7 cases (7/30). 26 cultures were positive of which 19 (19/26) were monomicrobial. Tuberculosis (TB) was implicated in 10 cases (10/30) including 4 cases in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spondylodiscitis; Mycobacterium tuberculosis; Staphylococcus aureus; Empirical treatment; Morocco.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400013

Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity
Provedor de dados: 58
Autores: Carlos,Lanamar de Almeida; Amaral,Kenas Aguiar da Silva; Vieira,Ivo José Curcino; Mathias,Leda; Braz-Filho,Raimundo; Samarão,Solange Silva; Vieira-da-Motta,Olney.
Staphylococci bacteria are involved in many human and animal infections and development of alternative antimicrobial drugs against pathogenic bacteria is of great interest to the pharmaceutical industry. This study investigated the in vitro effect of Rauvolfia grandiflora methanol extract (root bark fraction) (RGE) on the density of ATCC strains of Staphylococcus aureus and Staphylococcus epidermidis, and a clinical enterotoxin-producer, S. aureus bovine strain. The alkaloid, isoreserpiline, obtained from dichloromethane extract of R. grandiflora was ineffective against the strains tested. After incubation of staphylococci strains in the presence of 1.2 mg.mL-1 RGE, a significant inhibition of cell growth was observed using both spectrophotometry and ELISA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rauvolfia grandiflora; Staphylococcus aureus; Enterotoxin; Antimicrobial activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300011

Action of nisin and high pH on growth of Staphylococcus aureus and Salmonella sp. in pure culture and in the meat of land crab (Ucides cordatus)
Provedor de dados: 58
Autores: Grisi,Teresa Cristina S. de Lima; Gorlach-Lira,Krystyna.
The aim of this study was to evaluate the potential of nisin and high pH to inhibit the growth of Staphylococcus aureus and Salmonella sp. in broth culture and when inoculated into meat of land crab. In pure cultures, the growth of S. aureus was bly inhibited by nisin and the growth of Salmonella sp. was inhibited by nisin-EDTA (20 mM). The inhibition of S. aureus lasted for eight hours and Salmonella sp. growth was inhibited throughout the experiment (24 h). The high pH (pH 10.0 and 11.0 with NaHCO3-NaOH buffer) was very effective for in vitro inhibition of S. aureus and Salmonella sp. Nisin and high pH, when applied to the contaminated meat, did not yield the same effect. Nisin was not effective in preventing growth of both pathogens in the crab meat,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crab meat; Nisin; High pH; Staphylococcus aureus; Salmonella sp.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200010

Infecções intramamárias causadas por Staphylococcus aureus e suas implicações em paúde pública
Provedor de dados: 65
Autores: Fagundes,Helena; Oliveira,Carlos Augusto Fernandes.
Neste trabalho, são apresentados os principais problemas decorrentes das infecções intramamárias (mastites) causadas por Staphylococcus aureus e as conseqüências para a saúde humana da veiculação de suas toxinas através do leite. o S. aureus destaca-se como um dos microorganismos mais importantes que podem ser transmitidos através dos alimentos. Assim, discute-se a possibilidade de veiculação de gastroenterite estafilocócica, não somente através do consumo de leite cru contaminado, mas também de leite tratado termicamente ou de derivados lácteos contendo enterotoxinas termoestáveis. São apresentados alguns aspectos relacionados ao potencial toxigênico das cepas de S. aureus, bem como as principais características das enterotoxinas estafilocócicas....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Enterotoxinas; Mastite; Saúde humana.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000400058

Atividade antibacteriana de desinfetantes para uso na produção orgânica de aves
Provedor de dados: 65
Autores: Jaenisch,Fátima Regina Ferreira; Kuchiishi,Suzana Satomi; Coldebella,Arlei.
A determinação de produtos eficazes para a desinfecção e que não causem danos ao meio ambiente é um grande desafio para a avicultura orgânica. Neste trabalho foram avaliadas as atividades antibacterianas de quatro desinfetantes: ácido peracético, amônia quaternária, hipoclorito de sódio a 1% e a 0,1% de cloro ativo e do composto de ácidos orgânicos (cítrico, lático e ascórbico), frente às amostras padrão de Escherichia coli, Salmonella enteritidis e Staphylococcus aureus, na presença e ausência de matéria orgânica, sob duas diferentes temperaturas e tempo de contato de 20 minutos. Os ácidos orgânicos mostraram-se menos efetivos na presença de matéria orgânica. No entanto, o ácido peracético, na ausência desta, foi o mais eficaz frente à S. Enteritidis e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodegradável; Avicultura orgânica; Escherichia coli; Salmonella Enteritidis; Staphylococcus aureus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000200020

Mapeamento do perfil de resistência e detecção do gene mecA em Staphylococcus aureus e Staphylococcus intermedius oxacilina-resistentes isolados de espécies humanas e animais
Provedor de dados: 65
Autores: Coelho,Shana de Mattos de Oliveira; Moraes,Renata Amélia Menezes; Soares,Lidiane de Castro; Pereira,Ingrid Annes; Gomes,Ligia Portugal; Souza,Miliane Moreira Soares de.
Espécies de Staphylococcus spp resistentes a antimicrobianos representam um problema cosmopolita, sendo o controle de sua disseminação um importante desafio. O perfil de resistência a antimicrobianos de isolados de Staphylococcus aureus e Staphylococcus intermedius em amostras clínicas humanas e animais foi avaliado através do método de difusão em disco, no qual foi possível detectar um elevado nível de resistência à ampicilina e à penicilina. A avaliação da resistência à oxacilina, devido à heterogeneidade de resposta do gênero estudado, foi desenvolvida também através das seguintes técnicas: difusão em ágar modificado, ágar screen e microdiluição em caldo, e posteriormente correlacionada com a detecção do gene mecA, pela técnica de PCR, nas amostras...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Resistência bacteriana; Gene mecA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000100031

Intoxicação estafilocócica em restaurante institucional
Provedor de dados: 65
Autores: Rodrigues,Kelly Lameiro; Moreira,Angela Nunes; Almeida,Angela Terezinha Santiago; Chiochetta,Daiane; Rodrigues,Maria Joana; Brod,Claudiomar Soares; Carvalhal,José Beiro; Aleixo,José Antonio Guimarães.
Foi investigado um surto de intoxicação alimentar ocorrido em um restaurante institucional. Cinqüenta e seis pessoas, de um total de 88, foram acometidas de vômitos, diarréia, dores abdominais, prostração, febre e cefaléia após ingerirem uma refeição composta de sanduíche de galinha, refresco de laranja e pudim de leite. Os sintomas apareceram entre uma hora e meia e 12 horas após a ingestão da refeição e duraram 24 a 36 horas. As taxas de ataque específico foram de 64% para o sanduíche de galinha, de 61% para o refresco de laranja e de 60% para o pudim. A contagem de Staphylococcus aureus secretores de enterotoxina A no sanduíche de galinha foi de 2 X 10(8)UFC.g-1. Estes resultados, juntamente com os obtidos nos questionários aplicados às pessoas que...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Intoxicação alimentar; Staphylococcus aureus; Enterotoxina A.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000100049

Microbiologia de queijo tipo Minas Frescal produzido artesanalmente
Provedor de dados: 65
Autores: Loguercio,Andrea Pinto; Aleixo,José Antônio Guimarães.
Com o objetivo de avaliar as condições higiênico-sanitárias do queijo Minas Frescal produzido artesanalmente em Cuiabá - MT, foi realizada análise microbiológica de trinta amostras obtidas em dois pontos de comercialização. A análise microbiológica consistiu da contagem em placas de Staphylococcus aureus e da contagem estimativa de coliformes totais, fecais e Escherichia coli pela técnica do NMP-3 tubos. Na determinação de coliformes fecais, 28 amostras (93,33%) apresentaram número mais provável (NMP) &gt; 10² NMP/g e somente duas amostras (6,67%) estavam dentro dos padrões legais exigidos. Na contagem de S. aureus, em 29 amostras (96,67%) obteve-se valores superiores a 10³ufc/g, estando apenas 1 amostra (3,33%) em conformidade com o padrão legal....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Queijo Minas Frescal; Coliformes; Staphylococcus aureus; Escherichia coli.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000600024

Motilidade espermática e integridade acrossomal em doses de sêmen suíno refrigeradas e inoculadas com Esecherichia coli e Staphylococcus aureus
Provedor de dados: 65
Autores: Bennemann,Paulo Eduardo; Bortolozzo,Fernando Pandolfo; Wentz,Ivo; Cardoso,Marisa Ribeiro de Itapema.
Foram utilizados 12 ejaculados, coletados de maneira asséptica, distribuídos em sete tratamentos, sendo um grupo controle e os demais inoculados com três diferentes concentrações de S. aureus ou E. coli (5 x 10(5), 5 x 10(6) e 5 x 10(7) UFC/ml). Durante 96 horas, foram avaliados a motilidade espermática, o percentual de acrossomas intactos (NAR), o pH e o número de unidades formadoras de colônia (UFC/ml) das bactérias inoculadas. O desenvolvimento bacteriano foi decrescente ao longo das 96 horas de armazenamento. Com exceção do tratamento com a inoculação de 5 x 10(7) UFC de E. coli/ml, não foi observado efeito significativo das bactérias sobre a motilidade espermática (p&gt;0,05). Da mesma forma, não houve efeito significativo (p&gt;0,05) do S. aureus ou...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sêmen; Acrossoma; Escherichia coli; Staphylococcus aureus.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000200019

Variabilidades fenotípica e genotípica de estirpes de Staphylococcus aureus isoladas em casos de mastite subclínica bovina
Provedor de dados: 65
Autores: Ferreira,Luciano Menezes; Nader Filho,Antonio; Oliveira,Erika de; Zafalon,Luiz Francisco; Souza,Viviane de.
Foram submetidas a PCR-Ribotipagem e aos testes de sensibilidade in vitro frente a 12 antimicrobianos 77 estirpes de Staphylococcus aureus isoladas em amostras de leite procedentes de 40 vacas da raça holandesa que apresentaram mastite subclínica, em uma propriedade rural localizada no Estado de São Paulo, Brasil. Os resultados obtidos revelaram quatro diferentes padrões de resistência a antimicrobianos, sendo observada a predominância de resistência à lincomicina entre 19 (24,7%) estirpes de S.aureus. As 58 (75,3%) estirpes restantes foram sensíveis aos 12 antimicrobianos testados. A PCR-ribotipagem revelou a ocorrência de nove padrões genotípicos distintos, além de apresentar uma capacidade discriminatória maior (D = 0,82) que a obtida nos antibiogramas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mastite; Staphylococcus aureus; Variabilidade fenotípica e genotípica.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000400028

Single- and Multispecies Biofilms by Escherichia coli, Staphylococcus aureus, and Salmonella spp. Isolated from Raw Fish and a Fish Processing Unit
Provedor de dados: 65
Autores: Frozi,Jesieli Braz; Esper,Luciana Maria Ramires; Franco,Robson Maia.
ABSTRACT:  The consumption of fish by the Brazilian population is increasing. However, fish and seafood are highly perishable and can be contaminated with several microorganisms. In addition, the possibility of biofilm formation is a greater cause for concern. In this study, biofilm formation was evaluated in single- and multispecies cultures at 25°C for incubation periods of 0, 4, 8, 24, and 48h in stainless steel coupons (size, 1.0×1.0cm) immersed in tryptic soy broth. The characteristics of the formed biofilms after sanitizing by immersing the coupons in 200ppm sodium hypochlorite solution for 10min were also evaluated under the same experimental conditions but with some modifications. Biofilm structure was evaluated using scanning electron microscopy....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella spp.; Escherichia coli; Staphylococcus aureus; Biofilm; Stainless steel; Fish industry.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001000451

Detection by multiplex PCR of  Staphylococcus aureus ,  S. intermedius and  S. hyicus in artificially contaminated milk
Provedor de dados: 65
Autores: Gandra,Eliezer Avila; Fernandez,Maria Aparecida; Silva,Jorge Adolfo; Silva,Wladimir Padilha da.
ABSTRACT:  This research aimed to detect coagulase-positive Staphylococcus (CPS) directly in samples of artificially contaminated milk, using multiplex PCR (mPCR). Standard and isolated bacterial strains of S. aureus, S. epidermidis, S. hyicus, S. intermedius, Listeria monocytogenes and Escherichia coli species were used, evaluating the specificity and detection limit of mPCR, for artificially contaminated UHT milk. Primers specific for the nuc gene (NUC1-NUC2 were used for S. aureus, NUC3-NUC4 for S. hyicus and NUC5-NUC6 for S. intermedius). It was possible to detect the three target species by mPCR, directly from bovine whole milk, with adequate specificity and acceptable detention limit for identification of coagulase-positive Staphylococcus (CPS) in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Staphylococcus hyicus; Staphylococcus intermedius; Multiplex PCR; Nuc gene.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801418

Microbial quality of industrial and retail market grated parmesan cheese in the State of São Paulo, Brazil
Provedor de dados: 65
Autores: Aguilar,Carlos Eduardo Gamero; Rossi Junior,Oswaldo Durival; Vidal,Ana Maria Centola; Ribeiro,Laryssa Freitas; Rossi,Gabriel Augusto Marques.
ABSTRACT: With the objective to evaluate the hygienic and sanitary conditions of grated parmesan cheese acquired from the retail business, total 120 cheese samples were acquired: 60 of which were obtained from four different brands of cheese that were grated in factories, and the other 60 samples were obtained from another four brands of cheese that are normally acquired by retailers (supermarkets) in blocks and grated at the moment of sale. The population of heterotrophic mesophilic microorganisms ranged from 1.2×10³ to 1.1×107 colony-forming units (CFU)·g-1. All samples analyzed contained  Staphylococcus spp. with populations varying from 1.2×10³ to 8.7×106CFU·g-1, from which 60.0% were classified as coagulase-positive Staphylococcus and 52.5% of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy; Food microbiology; Public health; Staphylococcus aureus.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202257

Mastite com lesões sistêmicas por Staphylococus aureus subesp. aureus em coelhos
Provedor de dados: 65
Autores: Traverso,Sandra Davi; Cunha,Leonardo da; Fernandes,Joaquim César Teixeira; Loretti,Alexandre Paulino; Rhoden,Adriana; Wunder Jr.,Elsio; Driemeier,David.
Em uma criação composta por 1800 coelhos, 33% das matrizes apresentaram mastite e lesões cutâneas crostosas e purulentas. Estes animais apresentavam-se entre 10 a- 12 meses de idade e em segunda parição. Quinze coelhos afetados foram sacrificados e necropsiados. Na necropsia, além das lesões cutâneas haviam microabscessos em diversos órgãos. Das amostras coletadas isolou-se Staphylococcus aureus subesp. aureus. S. aureus subesp. aureus também foi isolado de "swab" nasal coletado do tratador encarregado de fazer o diagnóstico de gestação nas coelhas. Histologicamente, havia formação de múltiplos abscessos, presença de bactérias gram positivas em vasos sangüíneos e linfáticos, além de êmbolos bacterianos nos tecidos. Nas mamas, observou-se tecido glandular...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Staphylococcus aureus; Mastites; Lesões cutâneas; Coelhos.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000200031

DERMATITE ESTAFILOCÓCICA EM OVINO NO RIO GRANDE DO SUL
Provedor de dados: 65
Autores: Ribeiro,Luiz Alberto Oliveira; Rodrigues,Norma Centeno; Fallavena,Luiz Cesar Bello.
RESUMO Relata-se um caso de Dermatite Estafilocócica ocorrido em ovelha criada a campo no Rio Grande do Sul. São descritas e discutidas as lesões macroscópicas e microscópicas encontradas, assim como o isolamento do Staphylococcus aureus da lesão.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Dermatite estafilocócica; Ovinos; Rio Grande do Sul.
Ano: 1994
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000200035