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Bovine mastitis in the metropolitan area of Curitiba: antibiotic resistance and antimicrobial control of the infection BABT
Parada,José Luis; Gonçalves,Dicezar; Soccol,Vanete Tomaz; Lima,Marisa; Soccol,Carlos Ricardo.
A study from cows with mastitis was performed and Staphylococcus aureus was the predominant pathogen in 46.4 % among 153 studied strains from 276 milk samples of infected cows. Antibiotic resistance of 71 S. aureus isolates was determined in order to search resistant strains to antibiotics of clinical interest, as well as to determine their degree of multi-resistance. It was found that 60% of the S. aureus strains presented resistance to β-lactams, but none to oxacillin, teicoplamin or vancomycin. On the other hand, with the aim of reducing the use of current antibiotics and their associated resistance, a new formulation was introduced. The antimicrobial compounds (P22-P32), demonstrated to be effective in 55% of the 76 mastitis cases studied. The use of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mastitis; Staphylococcus aureus; Antibiotic resistance; Infection reduction.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400009
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Lactobacilli isolated from Algerian goat's milk as adjunct culture in dairy products BABT
Marroki,Ahmed; Bousmaha-Marroki,Leila.
In this study, nineteen Lactobacillus isolated from Algerian goat's milk, 13 belonging to L. plantarum, three to L. pentosus, two to L. rhamnosus and one to L. fermentum, were examined in vitro in order to be used as adjunct culture in dairy products. The strains were tested for their proteolytic activity, sensory and safety properties. Strains LbMS16 and LbMS21 L. plantarum and LbMF25 L. rhamnosus presented the highest proteolytic activity. All the tested lactobacilli were able to grow on MRS agar containing 0.5 and 1% (W/V) of oxgall, whereas none produced biogenic amine (BA) from the four tested amino acids and were resistant to pH 2.0 and 3.0, but some strains were able to grow at pH 3.5. None of examined strains were β-haemolytic when grown in hors...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus; Goat's milk; Safety aspect; Adjunct culture; Sensory properties; Antibiotic resistance.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300015
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Expression of the marA, soxS, acrB and ramA genes related to the AcrAB/TolC efflux pump in Salmonella entérica strains with and without quinolone resistance-determining regions gyrA gene mutations BJID
Ferrari,Rafaela Gomes; Galiana,Antonio; Cremades,Rosa; Rodríguez,Juan Carlos; Magnani,Marciane; Tognim,Maria Cristina Bronharo; Oliveira,Tereza C.R.M.; Royo,Gloria.
Several studies have been conducted in recent years to elucidate the structure, function and significance of AcrB, MarA, SoxS and RamA in Salmonella enterica. In this study, the relative quantification of acrB, soxS, marA and ramA genes expression was evaluated in 14 strains of S. enterica, with or without accompanying mutations in the quinolone resistance-determining regions of the gyrA gene, that were exposed to ciprofloxacin during the exponential growth phase. The presence of ciprofloxacin during the log phase of bacterial growth activated the genes marA, soxS, ramA and acrB in all S. enterica strains analyzed in this study. The highest expression levels for acrB were observed in strains with gyrA mutation, and marA showed the highest expression in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella sp.; Fluoroquinolone; Efflux pump; Antibiotic resistance.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200002
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Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenem BJID
Freitas,Ana Lúcia Peixoto de; Barth,Afonso Luis.
Susceptibility tests by disk diffusion and by E-test and molecular typing by macrorestriction analysis were performed to determine the relatedness of Pseudomonas aeruginosa isolates from three distinct hospitals. The resistance profile of 124 isolates to 8 antimicrobial agents was determined in three different hospitals, in Porto Alegre, Brazil. Frequencies of susceptibility ranged from 43.9% for carbenicillin to 87.7% for ceftazidime. Cross-resistance data of imipenem-resistant isolates indicated that most (70%) were also resistant to carbenicillin, although 30% remained susceptible to ceftazidime and cefepime. In general, susceptibility profiles were not able to determine relatedness among isolates of P. aeruginosa. On the other hand, molecular typing by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Antibiotic resistance; Imipenem.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000100001
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Vancomycin-resistant van a phenotype Enterococcus faecalis: first case in Minas Gerais state and epidemiological considerations BJID
Ribas,Rosineide Marques; Darini,Ana Lúcia da Costa; Moreira,Tomaz Aquino; Freitas,Claudete; Gontijo Filho,Paulo P..
We report the first case of vancomycin-resistant Enterococcus faecalis with VanA phenotype and vanA genotype isolated from a surgical patient in a general Hospital in Uberlândia, state of Minas Gerais, Brazil.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Nosocomial infections; Antibiotic resistance; VRE.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000400015
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Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms BJID
Sun,Erlin; Liang,Gehong; Wang,Lining; Wei,Wenjie; Lei,Mingde; Song,Shiduo; Han,Ruifa; Wang,Yubao; Qi,Wei.
Abstract Aims We sought to characterize the antibiotic susceptibility of strains of Stenotrophomonas maltophilia isolated from clinical samples, and the role of Stenotrophomonas maltophilia biofilm in antibiotic resistance. Methods Fifty-one clinical Stenotrophomonas maltophilia isolates were obtained from patients with nosocomial infection in the surgical wards and ICUs of six general hospitals in Tianjin, China. In vitro models of Stenotrophomonas maltophilia biofilms were established and confirmed by scanning electron microscopy and fluorescence microscopy with silver staining. The minimal inhibitory concentrations and biofilm inhibitory concentrations of commonly used antibiotics were determined. Results 47 of 51 strains were resistant to three or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stenotrophomonas maltophilia; Biofilm; Antibiotic resistance; Nosocomial.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400365
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Overview of tigecycline and its role in the era of antibiotic resistance BJID
Rossi,Flávia; Andreazzi,Denise.
The increasing antimicrobial resistance found in the many clinically important species of bacteria that commonly cause serious and life-threatening diseases presents a difficult challenge for clinicians, especially when an appropriate initial therapy must be chosen. New antibiotics are urgently needed to address the formidable issues associated with infections caused by vancomycin-resistant enterococci (VRE), methicillin-resistant Staphylococcus aureus (MRSA), penicillin-resistant Streptococcus pneumoniae and multidrug-resistant Gram-negative bacteria. The need for new antibiotics that effectively resist antimicrobial mechanisms of resistance has become paramount. Tigecycline is a new antimicrobial agent; it is the first in a new class of antibiotics, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tigecycline; Antibiotic resistance; Antimicrobial mechanisms.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000300010
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Ventilator-associated pneumonia in an adult clinical-surgical intensive care unit of a Brazilian university hospital: incidence, risk factors, etiology, and antibiotic resistance BJID
Rocha,Laura de Andrade da; Vilela,Carolina Assis Pereira; Cezário,Renata Cristina; Almeida,Alair Benedito; Gontijo Filho,Paulo.
The objective of this study was to evaluate clinical characteristics, etiology, and resistance to antimicrobial agents, among patients with ventilator-associated pneumonia (VAP). A case study vs. patients control under mechanical ventilation and hospitalized into clinical-surgical adults ICU of HC-UFU was performed from March/2005 to March/2006. Patients under ventilation for over 48 h were included in the study including 84 with diagnosis of VAP, and 191 without VAP (control group). Laboratory diagnosis was carried out through quantitative microbiological evaluation of tracheal aspirate. The identification of pathogens was performed by classical microbiological tests, and the antibiotics sensitivity spectrum was determined through the CLSI technique. VAP...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ventilator-associated pneumonia; Intensive care unit (ICU); Antibiotic resistance.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100017
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Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV and V isolated from healthy children attending public daycares in northeastern Brazil BJID
Carvalho,Suzi P. de; Almeida,Jéssica B. de; Andrade,Yasmin M.F.S.; Silva,Lucas S.C. da; Oliveira,Arianne C. de; Nascimento,Flávia S.; Campos,Guilherme B.; Oliveira,Márcio V.; Timenetsky,Jorge; Marques,Lucas M..
Abstract Nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) have increasingly been reported in healthy communities. This study aimed to assess the rate of S. aureus in general and MRSA in particular from nasal secretion of children in daycare centers in Vitória da Conquista, Brazil. The isolates were identified based on morphology, biochemical tests and by PCR. Detection of virulence genes, biofilm production, and susceptibility test by disk diffusion agar were performed. MRSA isolates were characterized by spa, SCCmec, and multilocus sequence typing (MLST). S. aureus were recovered from 70 (47.3%) of 148 children. Among the 11 MRSA strains (15.7%), two SCCmec types (IV and V) were detected. MLST identified four STs related to three...
Tipo: Info:eu-repo/semantics/other Palavras-chave: CA-MRSA; Antibiotic resistance; SCCmec; MLST.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400464
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Risk factors for pan-resistant Pseudomonas aeruginosa bacteremia and the adequacy of antibiotic therapy BJID
Tuon,Felipe F.; Gortz,Lucas W.; Rocha,Jaime L..
INTRODUCTION: The aim of this study was to determine risk factors for acquiring carbapenemresistant Pseudomonas aeruginosa bacteremia (CR-PA) and factors associated with in-hospital mortality. METHODS: Seventy-seven cases of bacteremia caused by P. aeruginosa were evaluated in a hospital with high incidence of CR-PA. Clinical and laboratorial factors, and previous use of antibiotics were also evaluated. In one analysis, CR-PA and carbapenem-susceptible P. aeruginosa (CS-PA) bacteremia were compared. A second analysis compared patients who died with survivors. RESULTS: Among 77 P. aeruginosa bacteremia, 29 were caused by CR-PA. Admission to the intensive care unit, higher number of total leukocytes, and previous use of carbapenem were statistically...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Imipenem; Meropenem; Antibiotic resistance; Risk factor.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000400007
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Increasing prevalence and dissemination of invasive nontyphoidal Salmonella serotype Typhimurium with multidrug resistance in hospitalized patients from southern Brazil BJID
Reis,Rafael Oliveira dos; Souza,Margarida Neves; Cecconi,Maria Cristina Piccoli; Timm,Loeci; Ikuta,Nilo; Simon,Daniel; Wolf,Jonas Michel; Lunge,Vagner Ricardo.
ABSTRACT Introduction: Nontyphoidal Salmonella serotypes are the main cause of human food-borne infection, including several hospitalization cases in the developing countries. Aim: To detect the main serotypes and to characterize the antibiotic resistance of human non-enteric and enteric nontyphoidal Salmonella from clinical isolates in Brazil. Methods: Salmonella serotypes were identified by microbiological and molecular methods. Susceptibility testing to antibiotics was performed by agar disk diffusion. Real-time PCRs were carried out for the detection of the genus Salmonella as well as serotypes Typhimurium and Enteritidis. Results: A total of 307 nontyphoidal Salmonella were isolated from 289 different patients in a reference laboratory (LACEN-RS)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Antibiotic resistance; Typhimurium; Enteritidis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000500424
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Enterobacteriaceae resistant to third generation cephalosporins upon hospital admission: risk factors and clinical outcomes BJID
Oliveira,Mirian Cristina; Oliveira,Clara Rodrigues Alves; Gonçalves,Karine Valéria; Santos,Marciléa Silva; Tardelli,Amanda Cristina Silva; Nobre Jr.,Vandack Alencar.
Objectives: Evaluate risk factors and clinical outcomes of infections caused by Enterobacteriaceae resistant to third-generation cephalosporins present in samples collected upon hospital admission.Methods: Risk factors were evaluated using a 1:2 ratio case-control study. Influence of resistance on the appropriateness of antibiotic therapy, length of stay, and hospital mortality were prospectively evaluated. Characteristics independently associated with the presence of resistant enterobacteria were assessed by logistic regression.Results: Enterobacteria resistant to third-generation cephalosporins were quite common (26.0%). Male gender (OR: 2.66; 95% CI, 1.17-5.06; p= 0.019), invasive prosthesis (OR: 3.79; 95% CI, 1.29-11.08; p= 0.015), previous use of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterobacteriaceae; Third generation cephalosporins; Risk factors; Antibiotic resistance.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000300239
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Clinical and microbiological observational study on AmpC β-lactamase-producing Enterobacteriaceae in a hospital of Nepal BJID
Baral,Pankaj; Neupanea,Sanjiv; Shresthac,Basudha; Ghimirea,Kashi Ram; Marasinia,Bishnu Prasad; Lekhaka,Binod.
Limited information is available regarding AmpC β-lactamase (ABL)-producing Enterobacteriaceae compared to extended-spectrum β-lactamase-producing enterobacteria. Since ABL-producing organisms are often resistant to multiple antimicrobial agents, therapeutic options against these pathogens are limited. Among 230 clinical Enterobacteriaceae isolates, 64 (27.8%) were found to produce ABL in our study. Escherichia coli (83.9%) was a predominant pathogen, followed by Citrobacter freundii (5.2%). A significant proportion of ABL-producing isolates (81.3%) were found to be multidrug resistant against commonly used antibiotics. Univariate analysis showed that prior history of taking antibiotics (odds ratio [OR], 5.278; confidence interval [CI], 2.838-9.817; p <...
Tipo: Info:eu-repo/semantics/other Palavras-chave: AmpC β-lactamase; Enterobacteriaceae; Antibiotic resistance.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200022
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Factors associated with bacteremia due to multidrug-resistant Gram-negative bacilli in hematopoietic stem cell transplant recipients BJMBR
Garnica,M.; Maiolino,A.; Nucci,M..
The epidemiology of bacteremia developing during neutropenia has changed in the past decade, with the re-emergence of Gram-negative (GN) bacteria and the development of multidrug resistance (MDR) among GN bacteria. We conducted a case-control study in order to identify factors associated with bacteremia due to multidrug-resistant Gram-negative (MDRGN) isolates in hematopoietic stem cell transplant recipients. Ten patients with MDRGN bacteremia were compared with 44 patients with GN bacteremia without MDR. Bacteremia due to Burkholderia or Stenotrophomonas sp was excluded from analysis (3 cases), because the possibility of intrinsical resistance. Infection due to MDRGN bacteria occurred in 2.9% of 342 hematopoietic stem cell transplant recipients....
Tipo: Info:eu-repo/semantics/other Palavras-chave: Hematopoietic stem cell transplant; Neutropenia; Gram-negative bacteria; Antibiotic resistance; Risk factors.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000300010
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BCG lymphadenopathy detected in a BCG-vaccinated infant BJMBR
Barouni,A.S.; Augusto,C.; Queiroz,M.V.N.P.; Lopes,M.T.P.; Zanini,M.S.; Salas,C.E..
Large-scale vaccination with BCG, the live attenuated strain of Mycobacterium bovis, is being adopted around the world, although sporadic complications have occurred after the procedure. Lymphadenopathy is not uncommon especially in babies under one year (0.73% of vaccinated infants), but the swelling subsides within 2 months in most cases, with no medical or surgical treatment. Brazil adopted BCG vaccination program earlier in the seventies and by 1995 more than 96% of the infant population received this immunization. We report here the occurrence of lymphadenopathy in a two-year-old child vaccinated with the Brazilian BCG strain. The diagnosis was made using a lymph node biopsy and intestinal aspirates that yielded a positive mycobacterial culture. The...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Bacille Calmette-Guérin (BCG); Vaccination; Polymerase chain reaction; Lymphadenopathy; Antibiotic resistance.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500011
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Incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater BJM
Alam,Mohammad Zubair; Aqil,Farrukh; Ahmad,Iqbal; Ahmad,Shamim.
This study reports the occurrence of antibiotic resistance and production of β-lactamases including extended spectrum beta-lactamases (ESβL) in enteric bacteria isolated from hospital wastewater. Among sixty-nine isolates, tested for antibiotic sensitivity, 73.9% strains were resistant to ampicillin followed by nalidixic acid (72.5%), penicillin (63.8%), co-trimoxazole (55.1%), norfloxacin (53.6%), methicillin (52.7%), cefuroxime (39.1%), cefotaxime (23.2%) and cefixime (20.3%). Resistance to streptomycin, chloramphenicol, nitrofurantoin, tetracycline, and doxycycline was recorded in less than 13% of the strains. The minimum inhibitory concentration (MIC) showed a high level of resistance (800-1600 µg/mL) to one or more antibiotics. Sixty three (91%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beta-lactamase; ESβL; Antibiotic resistance; Enteric bacteria; Plasmid transfer.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300021
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Safety, beneficial and technological properties of Enterococcus faecium isolated from Brazilian cheeses BJM
Santos,Karina Maria Olbrich dos; Vieira,Antônio Diogo Silva; Salles,Hévila Oliveira; Oliveira,Jacqueline da Silva; Rocha,Cíntia Renata Costa; Borges,Maria de Fátima; Bruno,Laura Maria; Franco,Bernadette Dora Gombossy de Melo; Todorov,Svetoslav Dimitrov.
This study aimed to characterize the safety and technological properties of Enterococcus faecium strains isolated from Brazilian Coalho cheeses. High levels of co-aggregation were observed between Enterococcus faecium strains EM485 and EM925 and both Escherichia coli and Clostridium perfringens. Both strains presented low levels of hydrophobicity. E. faecium EM485 and EM925 were both able to grow in the presence of 0.5% of the sodium salts of taurocholic acid (TC), taurodeoxycholic acid (TDC), glycocholic acid (GC), and glycodeoxycholic acid (GDC), although they showed the ability to deconjugate only GDC and TDC. Both strains showed good survival when exposed to conditions simulating the gastro intestinal tract (GIT). When tested for the presence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococcus faecium; Probiotic properties; Technological properties; Virulence factors; Antibiotic resistance.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100237
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Complete genome sequence of human pathogen Kosakonia cowanii type strain 888-76T BJM
Yang,Xue-Jing; Wang,Sai; Cao,Jun-Min; Hou,Jia-Hui.
ABSTRACT Kosakonia cowanii type strain 888-76T is a human pathogen which was originally isolated from blood as NIH group 42. In this study, we report the complete genome sequence of K. cowanii 888-76T. 888-76T has 1 chromosome and 2 plasmids with a total genome size of 4,857,567 bp and C+G 56.15%. This genome sequence will not only help us to understand the virulence features of K. cowanii 888-76T but also provide us the useful information for the study of evolution of Kosakonia genus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kosakonia cowanii; Genome sequence; Antibiotic resistance.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100016
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Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, Brazil BJM
Lemes-Marques,Eneida Gonçalves; Cruz,Cristina Durante; Destro,Maria Teresa.
Considering the lack of information available in Brazil concerning listeriosis, the present study aimed to analyze phenotypic and genotypically Listeria monocytogenes clinical isolates from the Southwestern region of the State of São Paulo, Brazil. From January 1995 to May 2005, thirteen isolates of L. monocytogenes from twelve patients with listeriosis were sent to the Adolfo Lutz Institute (Campinas Regional Laboratory, São Paulo, Brazil). The antimicrobial susceptibility of the isolates was evaluated by the microdilution broth method for ampicillin, gentamicin, trimethoprim, sulfamethoxazole and vancomycin. They were also serotyped (Denka Seiken, Japan), and sub-typed by PFGE employing ApaI and AscI and the American CDC protocol. None of the isolates...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; Serotypes; Antibiotic resistance; Genotypic characterization; Listeriosis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200019
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Bacteriocins produced by L. fermentum and L .acidophilus can inhibit cephalosporin resistant E .coli. BJM
Riaz,Saba; Nawaz,Syed Kashif; Hasnain,Shahida.
Reemerging infections occur due to resistant bacteria. Such infections create restrictions for clinicians and microbiologists in drug selection. Such problems demand new strategies for solution. Use of bacteriocins for this purpose may be fruitful. In the present research work, the inhibitory effects of bactericins on cephalosporin resistant Escherichia coli are used as model system for the control of antibiotic resistant pathogenic bacteria. Cephalosporin resistant Escherichia coli strain was isolated from pus by using conventional methodology. For bacteriocin production, Lactobacilli strains were selected by using selective media. Out of seventy two strains isolated from yogurt, fecal materials of human, chick, parrot and cat, only two strains (strain 45...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cephalosporin resistant Escherichia coli; Bacteriocin; Antibiotic resistance; Lactobacillus fermentum; Lactobacillus acidophilus.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300015
Registros recuperados: 48
Primeira ... 123 ... Última
 

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