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Changes in Follicular Vascularity during the First Follicular Wave in Lactating Cows OAK
ACOSTA, Tomas J.; HAYASHI, Ken-Go; MATSUI, Motozumi; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫.
Increase in the blood supply to individual follicles appears to be associated with follicular growth rates and the ability to become the dominant follicle, while reduced thecal vascularity appears to be closely associated with follicular atresia. Therefore, this study aimed to determine the real-time changes in the vascularity of the follicle wall during the first follicular wave in cycling Holstein cows. Normally cycling and lactating cows (n=5) were examined by transrectal color Doppler ultrasonography (the sensitivity for velocity: > 2 mm/sec) to determine the changes in the vasculature of the follicle wall (presence or absence of blood flow) and the diameter of follicles. A new follicular wave and ovulation were...
Palavras-chave: Blood flow; Follicular wave; Selection; Deviation; Cow.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2979
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In Vivo Evidence that Local Cortisol Production Increases in the Preovulatory Follicle of the Cow OAK
ACOSTA, Tomas J.; TETSUKA, Masa; MATSUI, Motozumi; SHIMIZU, Takashi; BERISHA, Bajram; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 清水, 隆; 宮本, 明夫.
The aim of the present in vivo study was to monitor real-time fluctuations of cortisol (Cr) in the wall of preovulatory follicles using a microdialysis system (MDS) implanted in the theca layer as well as changes in ovarian venous plasma (OVP) and jugular venous plasma (JVP). Seven cows were superovulated using FSH and prostaglandin F2α injections. Dialysis capillary membranes were surgically implanted into the theca layer of mature follicles and connected to a microdialysis system. Fractions of the perfusates were collected from Day -1 (Day 0=LH surge) to Day 3. No difference in the concentrations of Cr between JVP and OVP was detected throughout the experiment. Circulating concentrations of Cr ranged from 20 to 35 ng/ml 8 h after surgery in ovulatory and...
Palavras-chave: Bovine; Cortisol; Follicle; Ovary; Ovulation.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2980
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Time interval from ovulation to extrusion in female bullfrog in different photoperiods R. Bras. Zootec.
Agostinho,Claudio Angelo; Wechsler,Francisco Stefano; Castro,Cecilia Silva de; Agostinho,Lucas Miyabara; Ribeiro,Raphaela Rezende; Agostinho,Sueli Matiko Miyabara.
It was analyzed in this work the influence of photoperiod on time interval from ovulation induction period to extrusion of ovocits in female bullfrogs (Lithobates catesbeianus). It was used 54 females reared from metamorphosis to 9 months of age under three photoperiods: dark time (DL 0:24), 16 hours of daylight (DL 16:8) and 12 hours of daylight (DL 12:12). Ovulation was induced by intramuscular application of two doses of LHRHa with 12 hours of interval between the injections. After 10, 25, 28, 31, 34 and 37 hours from the first hormone injection, 10-gram samples (3,000 eggs) were extracted from each female at each time interval and fertilized. Egg hatching rate was checked in each...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artificial fertilization; Controlled reproduction; Fertilization rate; Frog reproduction; Lithobathes catesbeianus; Spawning.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000800001
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Validación y estandarización de una técnica para biopsia ovárica en el pargo flamenco, Lutjanus guttatus (Steindachner, 1869) OceanDocs
Alvarez-Lajonchère, L.; Ibarra-Zatarain, Z.; Ibarra-Castro, L..
Se estudió la estandarización y validación de una técnica de biopsia para la evaluación del desarrollo ovárico del pargo flamenco, Lutjanus guttatus (Steindachner, 1869). Ovocitos en diferentes estadios estaban presentes a lo largo de los ovarios. Los ovocitos vitelogénicos se distinguen por su opacidad bajo luz trasmitida. Los diámetros deben ser medidos dentro de las primeras 36 h después de su fijación en una solución de formalina al 1 % en 0,9 % de NaCl, antes de que su citoplasma sea clareado con solución Serra, para evitar distorsiones. Los ovocitos vitelogénicos mostraron una distribución de frecuencias de sus diámetros con una sola moda y sus medias no fueron significativamente diferentes entre los ovarios derecho e izquierdo (P > 0,05),...
Tipo: Journal Contribution Palavras-chave: Tests; Sampling (biological).
Ano: 2013 URL: http://hdl.handle.net/1834/4927
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Endocrine profiles and ovulation rate of cows superovulated with FSH following passive immunization against steroid free-bovine follicular fluid Braz. J. Vet. Res. Anim. Sci.
ALVAREZ,Rafael Herrera; CARVALHO,João Batista Pereira de; ROSA E SILVA,Alzira; PERONE,Cibele Nunes; RIBELA,Maria Tereza Carvalho Pinto; OLIVEIRA FILHO,Enoch Borges de.
Ten multiparous non-lactating cows were randomly assigned to one of two groups. On days 8 to 12 after estrus, the first group was given 100 ml antiserum against steroid-free bovine follicular fluid (anti-bFF), which was produced in ovariectomized sheep. The second group (control) was given 100 ml nonimmune ovariectomized sheep serum. Six hours after injection, both groups were superovulated with FSH (18 NIH-FSH-S1 units) and LH (0.29 NIH-LH-S1 units) in a 4-day decreasing regime. On the morning of the third day a luteolytic dose of prostaglandin F2alpha analogue (cloprostenol) was given. Artificial inseminations (AI) were done 48 and 60 h later. Embryos were collected 7 days after AI by a nonsurgical method. Blood samples were taken throughout the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superovulation; Follicular fluid; Passive immunization; Embryos; Cattle.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000600007
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The applications of timed artificial insemination and timed embryo transfer in reproductive management of dairy cattle R. Bras. Zootec.
Ambrose,Divakar Justus; Colazo,Marcos Germán; Kastelic,John Patrick.
Fertility of dairy cattle is generally decreasing. Overall estrus detection efficiency in lactating dairy cattle is low, as expression of estrus is often compromised. Consequently, undetected estrus, low AI-submission rates, and long inter-breeding intervals are the main contributors to poor reproductive efficiency. Although failure to become pregnant is the most common reason for culling dairy cattle, pregnancy rates could be improved by increasing the AI-submission rate through increased estrus detection efficiency, timed insemination (Timed-AI), or timed embryo transfer (Timed-ET). In these protocols, ovarian follicular development and luteolysis are controlled, culminating in synchronous ovulation in most cows. There...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cystic ovaries; Gonadotropin releasing hormone; Porcine luteinizing hormone; Progesterone; Synchronization of ovulation; Ultrasonography.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300042
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Superovulação em coelhas alimentadas com ração, contendo diferentes fontes de óleos vegetais - DOI: 10.4025/actascianimsci.v28i3.45 Animal Sciences
Andreazzi, Márcia Aparecida; Cesumar; Rigolon, Luiz Paulo; UEM; Cavalieri, Fábio Luiz Bim; Cesumar; Scapinello, Cláudio; UEM; Moraes, Gentil Vanini de; UEM; Faria, Haroldo Garcia de; UEM.
Effects of super-ovulation on 40 rabbit does were evaluated. Females were distributed in a completely randomized design, with four treatments (oil-free ration and rations with 3% canola oil, corn oil or soybean oil) and 10 replications. Each doe received a unique intramuscular dose of 40 UI of equine corionic gonadotrophin (eCG); 48 hours later they underwent breeding. After 72 hours of breeding, the does were slaughtered and 10mL blood samples were collected for progesterone and 17β-estradiol serum level analyses. Other parameters, such as weight of uterus with ovaries, number of follicles and of corpora lutea, were obtained. Embryos were recovered by three flushings, per uterine corn, with Dulbecco extender (PBS). The...
Palavras-chave: 5.04.03.00-1 Nutrição e Alimentação Animal coelhas; 17β-estradiol; Óleos poliinsaturados; Progesterona.
Ano: 2006 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/45
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Luteal dynamics in goats: morphological and endocrine features R. Bras. Zootec.
Arashiro,Eduardo Kenji Nunes; Viana,João Henrique Moreira; Fonseca,Jeferson Ferreira da; Camargo,Luiz Sérgio de Almeida; Fernandes,Carlos Antônio de Carvalho; Brandão,Felipe Zandonadi.
The aim of this study was to establish the morphologic and endocrine characteristics of luteal dynamics in goats. It was used Toggenburg female goats that showed natural estrus in a 48-hour interval. After estrus, ultrasonographic evaluations of the ovaries were daily performed during 21 days using a portable device (5MHz probe). Blood sample was collected for plasma progresterone (P4) determination. Corpora lutea were detected for the first time on day 5 and progressively increased in size until D9 (1.26 ± 0.08 cm²), with no variation on subsequent days. In females with one ovulation, the first visualization of the corpora lutea was earlier than in those with multiple ovulation (4.54 ±...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Corpus luteum; Goats; Progesterone; Ultrasonography.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000900011
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Polimorfismos de la proteína 15 morfogénica ósea (BMP15) asociados al tipo de parto en la oveja pelibuey. Colegio de Postgraduados
Argüello Hernández, Héctor Javier.
La Proteína 15 Morfogénica Ósea (BMP15), también conocida como Factor 9B de Crecimiento y Diferenciación (GDF9B), es miembro de la superfamilia β de factores de crecimiento (TGFβ) y su expresión en el ovocito es esencial para el desarrollo y crecimiento folicular. Diferentes polimorfismos en el gen BMP15 han sido asociados con el incremento de tasa ovulatoria y/o prolificidad en algunas razas de ovinos. De esta manera, los objetivos para este estudio fueron determinar tres polimorfismos (FecXG, FecXL y FecXH) con una mutación de una sola base (SNPs) en el gen BMP15, y estimar la asociación de los tres polimorfismos con el tipo de parto (prolificidad) en ovejas de la raza Pelibuey. Se tomaron muestras sanguíneas de la vena yugular de 253 ovejas en edad...
Palavras-chave: Polimorfismos; BMP15; Tetraprimer ARMs-PCR; Análisis de secuencias; Ovejas Pelibuey; Polymorphisms; Sequence analysis; Pelibuey ewes; Ganadería; Maestría.
Ano: 2013 URL: http://hdl.handle.net/10521/1958
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Induce spawning and larval reading of gilthead sea bream (sparus aurata)collected from fish farms, egypt OceanDocs
Aziz, F.K.; Zaki, M.I.; El-Absawy, M. El-G..
Sparus aurata females in captivity were injected with human chorionic gonadotropin hormone (HCG), pituitary extraction and both them during the spawning season. First hormonal treatment was applied as cumulative doses required for successful ovulation ranged from 4500 to 7500 IU\fish, the maximum fertilization percent was 71. The ovulation period under the influence of this treatment extended up to 14 days. In the second experiment, the total dose requirement for successful ovulation ranged from 2 to 4 pituitary extractions. Fish and the maximum fertilization percent recorded was 81, while the longest ovulation period...
Tipo: Journal Contribution Palavras-chave: Field Study; Biological fertilization; Spawning; Hatching; Larval development; Survival; Spawning; Hatching; Survival.
Ano: 2007 URL: http://hdl.handle.net/1834/1908
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Lecirelin and Buserelin (Gonadotrophin releasing hormone agonists) are equally effective for fixed time insemination in buffalo Braz. J. Vet. Res. Anim. Sci.
BARUSELLI,Pietro Sampaio; AMARAL,Renato; BARUFI,Francisco Bonomi; VALENTIM,Renato; MARQUES,Marcio de Oliveira.
Buffalo has peculiar reproductive patterns, which make artificial insemination programs a hard and expensive task. Artificial insemination in fixed time is advantaged because females show low incidence of homosexual behaviour and strong dominance relationships, which leads to a poor accuracy in estrus detection. The aim of this experiment was to compare the efficiency of two different GnRH agonists in the GnRH/PGF2alpha/GnRH protocol (Buserelin vs Lecirelin). Two hundred and seventy buffaloes with 45 to 60 days postpartum were synchronized and fixed-time inseminated. The animals were kept on pasture in two farms at São Paulo and Mato Grosso do Sul (Brazil). Cows in Group 1 (n = 132) received, intramuscularly, 20 µg of Buserelin at a random day of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Buffalo; Synchronization of ovulation; GnRH; Lecirelin; Buserelin.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962001000300009
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Half dose of prostaglandin F2a is effective to induce luteolysis in the synchronization of ovulation protocol for fixed-time artificial insemination in buffalo (Bubalus bubalis) Braz. J. Vet. Res. Anim. Sci.
Baruselli,Pietro Sampaio; Berber,Rodolfo Cassimiro de Araújo; Madureira,Ed Hoffman; Carvalho,Nelcio Antonio Tonizza de.
The objective of this study was to evaluate the efficiency of half dose of PGF2alpha in the synchronization of ovulation protocol (GnRH/PGF2alpha/GnRH) for fixed-time artificial insemination in buffalo. Two hundred and sixty two buffaloes (³40 days postpartum) were divided in 2 groups (Group 1, n = 125, GnRH/ ½ dose de PGF2alpha/GnRH; Control Group, n = 137, GnRH/PGF2alpha/GnRH). Animals in Group 1 received 25mg of GnRH IM (Lecirelin, Gestran-plus®) and, seven days later, half dose of PGF2alpha IM (75mg d-cloprostenol, Preloban®). Two days after PGF2alpha injection, all animals received 25mg of GnRH IM (Lecirelin). Animals in Control Group received the same treatment, except for the dose of PGF2alpha(150mg, IM,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Synchronization of ovulation; Dinoprost; Buffalo.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000600002
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Evaluation of synchronization of ovulation for fixed timed insemination in buffalo (Bubalus bubalis) Braz. J. Vet. Res. Anim. Sci.
Baruselli,Pietro Sampaio; Madureira,Ed Hoffman; Barnabe,Valquíria Hippollito; Barnabe,Renato Campanarut; Berber,Rodolfo Cassimiro de Araújo.
The objective of this study was to evaluate the efficiency of synchronization ovulation protocol (GnRH/PGF2alpha/GnRH) for fixed timed insemination in buffalo. In Experiment 1, thirty-three female buffaloes with 60 days postpartum, were divided in two groups for evaluation of the follicular dynamics (Group 1, n = 16, 10mg of GnRH/15mg of PGF2alpha/10mg of GnRH; Group 2, n = 17, 20mg of GnRH/15mg of PGF2alpha/10mg of GnRH). Ultrasonography exams were performed every 12 hours to verify the ovarian response to the first GnRH injection. Another evaluations were performed on PGF2a injection and second GnRH injection. After last hormonal injection, ultrasonography exams were performed every 6 hours to verify the moment of the...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Synchronization of ovulation; GnRH; Buffalo; Ovsynch.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000600007
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Probability density function of the number of embryos collected from superovulated Nelore breed donors Scientia Agricola
Beltrame,Renato Travassos; Barioni,Luis Gustavo; Quirino,Celia Raquel; Dantas,Ozanival Dario.
Several models have been developed to evaluate reproductive status of cows through concentration of progesterone in milk, the effect of sex selection in the commercial production of herds and bioeconomic performance of the multiple ovulation and embryo transfer system in select herds. However, models describing the production of embryos in superovulated females have yet to be developed. A probability density function of the number of embryos collected by donors of the Nelore breed was determined. Records of 61,928 embryo collections from 26,767 donors from 1991 to 2005 were analyzed. Data were provided by the Brazilian Association of Creators of Zebu and Controlmax Consultoria e Sistemas Ltda. The probability density...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zebu; Cattle; Reproduction; Model.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000400003
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Efeito da superovulação sobre o desempenho de bovinos doadores de embrião importados de clima temperado para clima tropical nos dois primeiros anos de adaptação Arq. Bras. Med. Vet. Zootec.
Bényei,B.; Barros,C.C.W..
Avaliou-se o desempenho de fêmeas bovinas doadoras de embrião nos primeiros dois anos de trabalho em um centro do programa multiple ovulation and embryo transfer (MOET). Utilizaram-se novilhas prenhes de recente importação de clima moderado para o Nordeste Brasileiro. Compararam-se os resultados de superovulação em 1996, primeiro ano de adaptação dos animais, e 1997 com AUSA-Superov (FSH) e Folligon (eCG). O número de CL contados no ovário foi 3,3 (FSH) e 4,2 (eCG) em 1996 e 8,2 (FSH) e 9,6 (eCG) em 1997, o de óvulos e embriões degenerados coletados de 2,8 (FSH) e 3,8 (eCG) em 1996 e 6,5 (FSH) e 7,4 (eCG) em 1997, o de embriões de boa qualidade de 1,9 (FSH) e 2,3 (eCG) em 1996 e 5,5 (FSH) e 4,0 (eCG) em 1997. A eficiência...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovino; Holandês; Estresse de calor; Aclimatação; Embrião; Superovulação.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400014
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Variações fisiológicas de parâmetros reprodutivos em vacas de raça Holandesa importadas da Hungria para o Nordeste brasileiro Braz. J. Vet. Res. Anim. Sci.
BÉNYEI,Balázs; BARROS,Costa Celso Walter.
O número de dias entre a parição e o primeiro cio, as medidas de volume dos ovários e os resultados de superovulação no desempenho de doadoras da raça Holandesa (n = 70) foram analisados com o objetivo de encontrar e determinar a possível influência do clima sobre esses dados. No primeiro ano do experimento (1996), levaram-se 112,1 ± 30,5 dias da parição até o primeiro cio, sendo assim um período irregular, pois o resultado da experiência do grupo testemunha foi 48 ± 12,0 dias. Os resultados do tamanho dos ovários e de superovulação foram de acordo com a teoria de que a atividade sexual das vacas doadoras pode ser influenciada pelo estresse causado pela aclimatação. As vacas foram superovuladas, os resultados foram modelados com análise de mínimo quadrado...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aclimatação; Superovulação; Estresse por calor; Gado Holandês; Reprodução.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000300008
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Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas ArchiMer
Bernay, Benoît; Baudy-floc'H, Michele; Zanuttini, Bruno; Zatylny, Céline; Pouvreau, Stephane; Henry, Joël.
For more than six decades, several studies have shown that genital products to entering the mantle cavity via the incurrent siphon, initiate in oyster, strong and rhythmic contractions of the adductor muscle (AM). In order to characterize the regulatory peptides capable of triggering AM contractions, we focused on the identification of putative myotropic peptides from genital products. Two experimental approaches were developed. The first one, based on a mass spectrometry screening of the male genital products, led to the identification of the tetrapeptide APGWamide. This neuropeptide was also detected in the seminal secretions of the cephalopod Sepia officinalis. In this species, APGWamide is directly involved in the oocyte transport. In Crassostrea, in...
Tipo: Text Palavras-chave: Mass spectrometry; Myotropin; APGWamide; Egg laying; Molluscan.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1697.pdf
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POST WEANING FEEDING MANAGEMENT AND PERFORMANCE OF MERINO EWES GRAZING ON NATURAL AND IMPROVED PASTURES AT MATING SEASON Ciência Rural
Bianchi,Gianni; Burgueño,Juan; Fernández Abella,Daniel; Garibotto,Gustavo; Cáceres,Rosario; Cesar,Rómulo; Jones,Guillermo.
In the uruguayan Basalt region, the reproductive performance of 300 Merino ewes and the effect of 3 post-weaning feeding managements (PWFM: I: 1.2 cm height of available forage and 16 ewes/ha; II: 2.03 cm height of available forage and 5 ewes/ha; III: 2.5cm height of available forage and 1 ewe/ha) as well as 2 feeding levels before and during the breeding season (FLM: native pastures: green DM/ha: 883kg, CP: 114g/kg DM, NDF: 781g/kg DM and improved pastures: DM /ha: 1270kg, CP: 194g/kg DM, NDF: 598g/kg DM) were studied with a factorial arrangement of treatments. The PWFM extended for 70 days, ewes were mated in the period from 13/4/96 to 13/5/96 and FLM were applied for 30 days, starting 15 days before beginning of breeding. There were not differences on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Body condition; Mating; Reproduction.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000100017
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Induction of ovulation and stimulation of spermation by LHRH or an LHRH analog associated or not with pimozide. Effects on some teleost fish. ArchiMer
Billard, R; Weil, C; Barnabe, G.
Effectiveness of LHRH and one of its analogs (LHRH-A = D-Ala super(6) ethylamide) on induction of ovulation and stimulation of spermiation is reviewed here. LHRH was also combined with pimozide, an anti-dopaminergic agent, to try to cancel dopamine inhibition of gonatotropin (GtH) release. LHRH-A alone (50 mu g/kg) did not initiate ovulation but only oocyte maturation. When pimozide (10 mg/kg) and LHRH-A (50 mu g/kg) were combined, gonadotropic hormone secretion was significantly increased, causing ovulation and stimulating spermiation. This combination advanced ovulation in trout. Trout egg quality was markedly altered by...
Tipo: Text Palavras-chave: Salmonidae; Dicentrarchus labrax; Sparidae; Cyprinidae; Teleostei; Pisces; Inhibitors; Sex hormones; Sexual maturity; Spermatogenesis; Ovulation; Induced breeding; Sexual reproduction.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1218.pdf
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Temperament and reproductive biology: emotional reactivity and reproduction in sheep R. Bras. Zootec.
Blache,Dominique; Bickell,Samantha L..
Reproductive capacity is controlled by a large number of factors such as season, social interactions and metabolic status. However, the influence of emotional reactivity on reproductive success has not been intensively investigated in farm animals. In this review, we define emotion reactivity and the expression of its inter individual variability named temperament. We briefly describe our protocol to measure temperament in sheep and discuss the heritability of temperament traits. Using the results obtained from our flock of sheep selected for calm or nervous temperament, we illustrate how this selection affects the reproductive biology from changing the inexperienced ewe's response to the male effect to improving lamb survival and
Tipo: Info:eu-repo/semantics/article Palavras-chave: Male effect; Ovulation; Sheep; Survival; Temperament.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300044
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