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5-Aminolevulinic acid photoactivated over planktonic and biofilm forms of Enterococcus faecalis as a pharmacological therapy alternative BJPS
Beltran-Leal,Adriana; Muñoz-Ruiz,Abraham; Esparza-Villalpando,Vicente; Castro,Yolanda; Pozos-Guillen,Amaury; Flores,Héctor.
The purpose of the study was to evaluate the antibacterial effect of protoporphyrin IX (PpIX) generated by the exogenous administration of 5-aminolevulinic acid or δ-ALA and activated with an argon laser over a planktonic and biofilm of Enterococcus faecalis (E. faecalis) as a pharmacological therapy alternative. A planktonic strain of E. faecalis was cultured with a solution of ∂-ALA (40 µg/mL)-thioglycolate solution for 13 min, and a biofilm of E. faecalis was cultured in a δ-ALA (80 µg/mL)-thioglycolate solution for 13 min. Then, both were irradiated with an argon laser. Finally, the antibacterial effect was evaluated by counting the CFU in planktonic form, and a LIVE/DEAD viability cell test. The production and accumulation of PpIX from exogenously...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm; Photodynamic therapy; 5-aminolevulinic acid.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100572
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5-Bromo-2’-deoxyuridine induces visible morphological alteration in the DNA puffs of the anterior salivary gland region of Bradysia hygida (Diptera, Sciaridae) BJMBR
de Almeida,J.C.; Sauaia,H.; Viana,J.C..
5-Bromo-2’-deoxyuridine (BrdUrd) has long been known to interfere with cell differentiation. We found that treatment ofBradysia hygida larvae with BrdUrd during DNA puff anlage formation in the polytene chromosomes of the salivary gland S1 region noticeably affects anlage morphology. However, it does not affect subsequent metamorphosis to the adult stage. The chromatin of the chromosomal sites that would normally form DNA puffs remains very compact and DNA puff expansion does not occur with administration of 4 to 8 mM BrdUrd. Injection of BrdUrd at different ages provoked a gradient of compaction of the DNA puff chromatin, leading to the formation of very small to almost normal puffs. By immunodetection, we show that the analogue is preferentially...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA puffs; BrdUrd; Gene amplification; Polytene chromosomes; Salivary glands; Sciaridae.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200002
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5-HT1A autoreceptor modulation of locomotor activity induced by nitric oxide in the rat dorsal raphe nucleus BJMBR
Gualda,L.B.; Martins,G.G.; Müller,B.; Guimarães,F.S.; Oliveira,R.M.W..
The dorsal raphe nucleus (DRN) is the origin of ascending serotonergic projections and is considered to be an important component of the brain circuit that mediates anxiety- and depression-related behaviors. A large fraction of DRN serotonin-positive neurons contain nitric oxide (NO). Disruption of NO-mediated neurotransmission in the DRN by NO synthase inhibitors produces anxiolytic- and antidepressant-like effects in rats and also induces nonspecific interference with locomotor activity. We investigated the involvement of the 5-HT1A autoreceptor in the locomotor effects induced by NO in the DRN of male Wistar rats (280-310 g, N = 9-10 per group). The NO donor 3-morpholinosylnomine hydrochloride (SIN-1, 150, and 300 nmol) and the NO scavenger...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Dorsal raphe nucleus; Nitric oxide; Serotonin; 5-HT1A receptor; Locomotor activity.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400010
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5-Methyltetrahydrofolate-homocysteine methyltransferase gene polymorphism (MTR) and risk of head and neck cancer BJMBR
Galbiatti,A.L.S.; Ruiz,M.T.; Biselli-Chicote,P.M.; Raposo,L.S.; Maniglia,J.V.; Pavarino-Bertelli,E.C.; Goloni-Bertollo,E.M..
The functional effect of the A>G transition at position 2756 on the MTR gene (5-methyltetrahydrofolate-homocysteine methyltransferase), involved in folate metabolism, may be a risk factor for head and neck squamous cell carcinoma (HNSCC). The frequency of MTR A2756G (rs1805087) polymorphism was compared between HNSCC patients and individuals without history of neoplasias. The association of this polymorphism with clinical histopathological parameters was evaluated. A total of 705 individuals were included in the study. The polymerase chain reaction-restriction fragment length polymorphism technique was used to genotype the polymorphism. For statistical analysis, the chi-square test (univariate analysis) was used for comparisons between groups and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Head and neck cancer; Polymorphism; Folate metabolism; MTR gene.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500005
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5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers Genet. Mol. Biol.
Alves-Costa,Fernanda A.; Martins,Cesar; Matos,Fernanda Del Campos de; Foresti,Fausto; Oliveira,Claudio; Wasko,Adriane P..
In order to extend the genetic data on the Sciaenidae fish family, the present study had the purpose to characterize PCR-generated 5S rDNA repeats of twelve species of this group through PAGE (Polyacrylamide Gel Electrophoresis) analysis. The results showed the occurrence of at least two different 5S rDNA size classes in all the species. Moreover, 5S rDNA repeats of one of the studied species - Isopisthus parvipinnis - were cloned and subjected to nucleotide sequencing and Southern blot membrane hybridization analyses, which permitted to confirm the existence of two major 5S rDNA classes. Phylogenetic analysis based on the nucleotide sequences of different 5S rDNA repeats of I. parvipinnis lead to their separation into two major clusters. These results may...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sciaenidae; Fish; Isopisthus parvipinnis; 5S rDNA; Molecular markers.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200025
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5th Food Safety and Security Symposium: food and health Ciênc. Tecnol. Aliment.
NITZKE,Julio Alberto; CLADERA,Florencia; THYS,Roberta; DA CRUZ,Fabiana Thomé; MARTINELLI,Sônia.
Abstract The 5º Simpósio de Segurança Alimentar (5th Food Safety and Security Symposium) was held in May, 2015, in Bento Gonçalves, RS, Brazil with the objective of discussing the interrelation between food and health, in various perspectives. This paper reviews the state of the art regarding all the issues discussed during the Symposium, connecting them with the lectures presented at the conference. As final remarks, it was perceived that the interrelation between food and health is growing stronger and cannot be discussed without involving the different areas involved.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Food safety; Food security; Food and health.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000500001
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6 weeks consumption of pure fresh coconut milk caused up-regulation of eNOS and CSE protein expression in middle-aged male rats BJPS
Jansakul,Chaweewan; Naphatthalung,Jomkarn; Pradab,Sakda; Yorsin,Somruedee; Kanokwiroon,Kanyanatt.
Coconut milk (CCM) has been an important cooking ingredient in the Asia-Pacific region since ancient time. Due to its high content of saturated fatty acids, it has been considered atherogenic. We have tested if chronic consumption of fresh coconut milk by middle-aged male rat affects vascular function, plasma glucose and lipid profiles. Compared to control, CCM caused lower maximal contraction to phenylephrine of thoracic aortic rings and increased relaxation to acetylcholine that was abolished by N G-nitro-L-arginine (L-NA) or disruption of the endothelium. DL-propargylglycine caused slight increase in baseline tension of L-NA treated aortic rings of CCM-treated rats and produced higher contractile response of the aortic rings to low concentrations of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coconut milk; NO. H2S; Plasma glucose; Thoracic aorta.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300608
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60Co GAMMA RADIATION EFFECTS ON Apis mellifera VENOM: BIOCHEMICAL, PHARMACOLOGICAL, AND IMMUNOLOGICAL ASPECTS J. Venom. Anim. Toxins
Costa,H..
Tipo: Info:eu-repo/semantics/review
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100016
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6-Benzilaminopurina e ácido indolbutírico na multiplicação in vitro da amoreira - preta (Rubus idaeus L.), cv. Tupy Ciência Rural
Erig,Alan Cristiano; De Rossi,Andrea; Fortes,Gerson Renan de Luces.
O presente trabalho avaliou o efeito de diferentes níveis de BAP (0; 2; 4; 6; 8 e 10miM) e de AIB (0; 0,5 e 1miM) na multiplicação in vitro da amoreira-preta cv. Tupy. O meio utilizado foi o MS suplementado com 100mg.<IMG SRC="http:/img/fbpe/cr/v32n5/11864s1.gif">-1 de mio-inositol, 30g.<IMG SRC="http:/img/fbpe/cr/v32n5/11864s1.gif">-1 de sacarose e 6g.<IMG SRC="http:/img/fbpe/cr/v32n5/11864s1.gif">-1 de ágar. Após a inoculação, as culturas foram mantidas em sala de cultura com 16 horas de fotoperíodo, temperatura de 25 ± 2ºC e radiação de 25µmoles.m-2.s-1. O número de gemas e o número de brotações foram avaliados com intervalo semanal, num total de quatro avaliações, e, na última avaliação, considerou-se a altura da brotação, bem como...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reguladores de crescimento; Micropropagação; Cultura de tecidos.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000500005
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6-Bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathway Anais da ABC (AABC)
ZHAO,XIAO-E; YANG,ZHENSHAN; GAO,ZHEN; GE,JUNBANG; WEI,QIANG; MA,BAOHUA.
This study aimed to investigate how 6-bromoindirubin-3’-oxime (BIO) increases the osteogenic differentiation of canine bone mesenchymal stem cells (BMSCs) and the role of the Wnt/β-catenin signaling pathway in this process. We mimicked the effect of Wnt by adding BIO to the culture medium of BMSCs and examined whether canonical Wnt signaling positively affects the differentiation of these cells into osteoblasts. Canine BMSCs were cultured with 0.5 and 1.0 μM BIO under osteogenic conditions and then differentiation markers were investigated. It was found that BIO significantly increased the activity of alkaline phosphatase (ALP), the number of ALP-positive cells, the mineralization level and calcium deposits. Moreover, cells cultured with 0.5 and 1.0 μM...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bone mesenchymal stem cells; Bromoindirubin oxime; Osteogenetic differentiation; Canine.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100610
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6-Gingerol inhibits hair cycle via induction of MMP2 and MMP9 expression Anais da ABC (AABC)
HOU,CHUN; MIAO,YONG; JI,HANG; WANG,SUSHENG; LIANG,GANG; ZHANG,ZHIHUA; HONG,WEIJIN.
ABSTRACT 6-Gingerol is the major active constituent of ginger. In the current study, we aimed to investigate the mechanisms underlying the effects of 6-Gingerol on hair growth. Mice were randomly divided into five groups; after hair depilation (day 0), mice were treated with saline, or different concentrations of 6-Gingerol for 11 days. The histomorphological characteristics of the growing hair follicles were examined after hematoxylin and eosin staining. The results indicated that 6-Gingerol significantly suppressed hair growth compared with that in the control group. And choose the concentration of 6-Gingerol at 1 mg/mL to treated with mice. Moreover, 6-Gingerol (1 mg/mL) significantly reduced hair re-growth ratio, hair follicle number, and hair follicle...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MMP2; MMP9; 6-Gingerol; Hair growth.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602707
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6-n-propyltiouracil (PROP) taster status in Brazilian adults Ciênc. Tecnol. Aliment.
Von Atzingen,Maria Carolina Batista Campos; Silva,Maria Elisabeth Machado Pinto e.
The objective of this study was to determine PROP (6-n-propyltiouracil) taster status in adults and its relationship with anthropometric variables and pleasantness of sugar, salt, and fat. A total of 123 subjects rated the intensity of PROP and sodium cloride (NaCl) solutions using the labeled magnitude scale. For pleasantness evaluation, it was used concentrated orange juice (sugar) and mashed potato (salt and fat). The subjects were classified as non-tasters (n = 35), medium-tasters (n = 33) and super-tasters (n = 55). In this study, no relationship was found between PROP taster status and age, sex, weight, body mass index, and pleasantness. Although genetic markers may influence the degree of liking of certain foods, one must consider that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propylthiouracil; Body mass index; Pleasantness.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000400006
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6o Congresso Brasileiro de Agroinformática - SBIAgro 2007. Infoteca-e
TERNES, S..
bitstream/item/159096/1/Boletim-6-Novembro-2007.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E) Palavras-chave: SBIAgro; Congresso.
Ano: 2007 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/7889
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70 years of development of Pharmaceutical Sciences: a lot to celebrate! BJPS
Aranha,Leila Rangel de Carvalho.
Tipo: Info:eu-repo/semantics/other
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200001
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7-epi-griffonilide, a new lactone from Bauhinia pentandra: complete 1H and 13C chemical shift assignments Anais da ABC (AABC)
ALMEIDA,MACIA C.S. DE; SOUZA,LUCIANA G.S.; FERREIRA,DANIELE A.; PINTO,FRANCISCO C.L.; OLIVEIRA,DÉBORA R. DE; SANTIAGO,GILVANDETE M.P.; MONTE,FRANCISCO J.Q.; BRAZ-FILHO,RAIMUNDO; LEMOS,TELMA L.G. DE.
ABSTRACT A new lactone, 7-epi-griffonilide (1), and six known compounds, 2, 3a - 3c, 4a and 4b, were isolated from the leaves of Bauhinia pentandra (Fabaceae). The structures elucidation of 1 and 2 were based on detailed 2D NMR techniques and spectral comparison with related compounds, leading to complete assignment of the 1H and 13C NMR spectra.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bauhinia pentandra; Fabaceae; Cyanoglucoside; Lactones; RMN..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100065
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7-hydroxycalamenene effects on secreted aspartic proteases activity and biofilm formation of Candida spp. Repositório Alice
AZEVEDO, M. M. B.; ALMEIDA, C. A.; CHAVES, F. C. M.; RODRIGUES, I. A.; BIZZO, H. R.; ALVIANO, C. S.; ALVIANO, D. S..
The 7-hydroxycalamenenene-rich essential oil (EO) obtained from the leaves of Croton cajucara (red morphotype) have been described as active against bacteria, protozoa, and fungi species. In this work, we aimed to evaluate the effectiveness of 7-hydroxycalamenenene against Candida albicans and nonalbicans species.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Croton cajucara; Anti-candidal activity; Essential oil; Óleo essencial; Secreted aspartic proteases.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1040252
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7-pentoxyresorufin-o-depentilase and 7-Benzyloxyresorufin-o-debenzylase as potential biomarkers of exposure to 17B-estradiol in nile tilapia. Repositório Alice
BITTENCOURT, F. de O.; FRIGHETTO, R. T. S.; QUEIROZ, J. F. de; LOSEKANN, M. E.; LUIZ, A. J. B..
2009
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Biomarcadores; Poluição da água.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/579352
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"80 anos a Serviço da Pátria" Anais da ESALQ
Lordello,Luiz Gonzaga E..
Tipo: Info:eu-repo/semantics/other
Ano: 1981 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0071-12761981000100001
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-866G/A and Ins/Del polymorphisms in the UCP2 gene and diabetic kidney disease: case-control study and meta-analysis Genet. Mol. Biol.
Dieter,Cristine; Assmann,Taís Silveira; Lemos,Natália Emerim; Massignam,Eloísa Toscan; Souza,Bianca Marmontel de; Bauer,Andrea Carla; Crispim,Daisy.
Abstract Uncoupling protein 2 (UCP2) decreases reactive oxygen species (ROS). ROS overproduction is a key contributor to the pathogenesis of diabetic kidney disease (DKD). Thus, UCP2 polymorphisms are candidate risk factors for DKD; however, their associations with this complication are still inconclusive. Here, we describe a case-control study and a meta-analysis conducted to investigate the association between UCP2 -866G/A and Ins/Del polymorphisms and DKD. The case-control study comprised 385 patients with type 1 diabetes mellitus (T1DM): 223 patients without DKD and 162 with DKD. UCP2 -866G/A (rs659366) and Ins/Del polymorphisms were genotyped by real-time PCR and conventional PCR, respectively. For the meta-analysis, a literature search was conducted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: UCP2; Polymorphisms; Diabetic kidney disease.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400101
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8-Propyl-6H-[1,3]dioxolo[4,5-g]chromen-6-one: A new coumarin with monoamine oxidase B inhibitory activity and possible anti-parkinsonian effects BJPS
Olaya,María del Pilar; Vergel,Nadezdha Esperanza; López,Jose Luis; Viña,Dolores; Guerrero,Mario Francisco.
Parkinson’s disease is a common neurodegenerative disorder. In this study, the monoamine oxidase inhibitory activity and potential anti-parkinsonian effects of 8-propyl-6H-[1,3]dioxolo[4,5-g]chromen-6-one (FCS303), a new synthetic coumarin, were evaluated. To do this, we used the reserpine model of Parkinson’s disease, an assay of levodopa/carbidopa potentiation, the catalepsy model of haloperidol, and an in vitro assay against monoamine oxidase (MAO) activity. Additionally, lipid peroxidation and protein carbonyl group quantification was performed in mice brain homogenates previously treated with haloperidol. FCS303 inhibited monoamine oxidase B (MAO-B) with an IC50 of 5.46 ± 0.36 µM; however, there was no effect on monoamine oxidase A (MAO-A). The oral...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Parkinson’s disease; Monoamine oxidase B; Coumarin; Mice; Reserpine; Levodopa; Carbidopa.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100508
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