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Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds
Provedor de dados: 63
Autores: Popovic,Tatjana; Milovanovic,Predrag; Aleksic,Goran; Gavrilovic,Veljko; Starovic,Mira; Vasic,Mirjana; Balaž,Jelica.
Halo blight, caused by Pseudomonas savastanoi pv. phaseolicola (Psp), is considered to be an important bacterial disease on common bean (Phaseolus vulgaris L.) in Serbia. Use of pathogen-free seeds is one of the most effective control measures against this disease. The aim of this study was to evaluate a detection method for Psp on untreated common bean seeds (23 genotypes) from commercial crops grown within Serbia. Detection of this pathogen was made by plating onto the modified sucrose peptone (MSP) and Milk Tween (MT) semi-selective mediums from soaked whole common bean seed. Colonies growing on the MSP medium were light yellow, convex and shiny, whereas on the MT medium, they were creamy white, flat and circular. The pathogenicity of the obtained...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MSP; MT; ELISA; Nested-PCR; Halo blight; Pathogenicity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400005