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The canavanine-pentacyanoamine ferrate complex formation. Repositório Alice
BRUNE, W.; FABRIS, J. D.; JOSE, S..
The complexation of pentacyanoamine ferrate with canavanine is not a specific one. Other amino acids, especially tryptophan and histidine may also react with proper analytical features. Se also do the indolyl and imidazolyl moieties of nonamino acids. Photoactivation does neither seem to occur by exposure of the pentacyanoamine ferrate reagent to daylight, nor is it a perequisite for a more sensitive response of canavanine complexation. On the other hand, oxidants and reducers may well interfere with the absorbance of the complex. In addition molar absorbance, stoichiometric ratio of the complex, and reation-order kinetics were determined. By mean of temperature programming equilibrion constant andreaction kinetics, enthalpy, Gibbs nenergy, entropy values,...
Tipo: Artigo de periódico Palavras-chave: Oxido de Ferro.; Iron; Oxides..
Ano: 1988 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/478718
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Parameter evaluation in Michaelis-menten kinetics. Repositório Alice
BRUNE, W.; FABRIS, J. D..
Parameter estimation reliability in enzyme kinetics depends upon the substrate range concentrations under assay. An inappropriate concentration set may lead to spurious values of km and Vmax in the Michaelis-Menten approach. In this paper, the theoretical arguments for a practical criterium concerning the best work range of substrate concentration are discussed on a velocity ratio basis (V1/Vn) as response to the pertinent substrate concentration ratio (S1/Sn).
Tipo: Artigo de periódico Palavras-chave: Química.
Ano: 1988 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/478714
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