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In vitro culture, PCR , and nested PCR for the detection of Theileria equi in horses submitted to exercise 45
Baldani,C.D.; Canola,P.A.; Neto,J.C.L.; Machado,R.Z..
This study compared the usefulness of in vitro culture, PCR, and nested PCR for the diagnosis of Theileria equi in horses submitted to stress during exercise. Blood samples from 15 apparently healthy horses, previously conditioned to a high-speed equine treadmill, were taken prior to and after exercise. The animals were divided into two experimental groups: 30-day training schedule (G1) and 90-day training schedule (G2). Statistical analysis was performed using a chi-square test and kappa statistic was used in order to assess agreement. No significant difference was observed between samples collected at resting or after exercise. In G1, merozoites of T. equi were detected in the blood smears of four horses before in vitro culture, whereas 14 samples were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eqüino; Theileria equi; Diagnóstico; Cultivo in vitro; PCR.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300005
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