Consulta: creatorFacets:"Belfort Jr.,R."
Registros recuperados: 3
Data/hora: 26/07/2024 22:22:23

Dose-dependent in vitro inhibition of rabbit corneal matrix metalloproteinases by an extract of Pothomorphe umbellata after alkali injury
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Barros,L.F.M.; Barros,P.S.M.; Röpke,C.D.; Silva,V.V.; Sawada,T.C.H.; Barros,S.B.M.; Belfort Jr.,R.
The in vitro ability of Pothomorphe umbellata ethanolic crude extract to inhibit matrix metalloproteinase (MMP) in normal cornea and in cornea after alkali injury was demonstrated. Corneas of albino rabbits were injured with 1 N NaOH for 20 s. After 48 h the corneas were excised, homogenized and analyzed for MMP-9 (92 kDa), pro-MMP-2 (72 kDa) and MMP-2 (67 kDa) activity by gelatin zymography. The activity was also measured in untreated corneas. After electrophoresis of 20 µg protein, gels were incubated with 50, 100, or 250 µg/mL lyophilized hydroethanolic (1:1) root crude extract of P. umbellata standardized for 4-nerolidylcatechol (7.09%). The activity of the enzymes was compared with that of untreated gel. At 48 h after injury, the activity of all MMPs...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cornea; Alkali injury; Metalloproteinases; Pothomorphe umbellata.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800014

Lack of evidence for superantigen activity of Toxoplasma gondii towards human T cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Vallochi,A.L.; Yamamoto,J.H.; Schlesinger,D.; Machado,M.A.C.; Silveira,C.; Martins,M.C.; Belfort Jr.,R.; Kalil,J.; Rizzo,L.V.
Toxoplasma gondii is an obligatory intracellular parasite whose life cycle may include man as an intermediate host. More than 500 million people are infected with this parasite worldwide. It has been previously reported that T. gondii contains a superantigen activity. The purpose of the present study was to determine if the putative superantigen activity of T. gondii would manifest towards human T cells. Peripheral blood mononuclear cells (PBMC) from individuals with no previous contact with the parasite were evaluated for proliferation as well as specific Vß expansion after exposure to Toxoplasma antigens. Likewise, PBMC from individuals with the congenital infection were evaluated for putative Vß family deletions in their T cell repertoire. We also...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma; Tolerance; Superantigen; T cell receptor.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800008

Treatment of cytomegalovirus retinitis with an intraocular sustained-release ganciclovir implant
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Muccioli,C.; Belfort Jr.,R.
The objective of this prospective study was to evaluate the efficacy and complications of the use of an intraocular sustained-release ganciclovir implant for the treatment of active cytomegalovirus (CMV) retinitis in AIDS patients. Thirty-nine eyes of 26 patients were submitted to ocular surgery. All patients underwent complete ocular examination before and after surgery. The surgical procedure was always done under local anesthesia using the same technique. The mean time for the surgical procedure was 20 min (range, 15 to 30 min). The average follow-up period was 3.7 months. Of all patient, only 4 presented recurrence of retinitis after 8, 8, 9 and 2 months, respectively. Three of them received a successful second implant. All 39 eyes of the 26 patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytomegalovirus; Retinitis; Intraocular inflammation; AIDS; Uveitis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700008