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Water extracts of Brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L. 42
Farias,Davi F.; Cavalheiro,Mariana G.; Viana,Martônio P.; Queiroz,Vanessa A.; Rocha-Bezerra,Lady C.B.; Vasconcelos,Ilka M.; Morais,Selene M.; Carvalho,Ana F.U..
This study assessed the toxicity of seed water extracts of 15 leguminous species upon Aedes aegypti larvae. A partial chemical and biochemical characterization of water extracts, as well as the assessment of their acute toxicity in mice, were performed. The extracts of Amburana cearensis, Anadenanthera macrocarpa, Dioclea megacarpa, Enterolobium contortisiliquum and Piptadenia moniliformis caused 100% of mortalit y after 1 to 3 h of exposure. They showed LC50 and LC90 values ranging from 0.43 ± 0.01 to 9.06 ± 0.12 mg/mL and from 0.71 ± 0.02 to 13.03 ± 0.15 mg/mL, respectively. Among the secondary metabolite constituents, the seed water extracts showed tannins, phenols, flavones, favonols, xanthones, saponins and alkaloids. The extracts also showed high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Larvicidal compounds; Leguminous seeds; Water extracts.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300006
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Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants 42
Ferreira,Paulo Michel P.; Farias,Davi F.; Viana,Martônio P.; Souza,Terezinha M.; Vasconcelos,Ilka M.; Soares,Bruno M.; Pessoa,Cláudia; Costa-Lotufo,Letícia V.; Moraes,Manoel O.; Carvalho,Ana F.U..
This study assessed the antiproliferative and cytotoxic potential against tumor lines of ethanolic seed extracts of 21 plant species belonging to different families from Northeastern Brazil. In addition, some underlying mechanisms involved in this cytotoxicity were also investigated. Among the 21 extracts tested, the MTT assay after 72 h of incubation demonstrated that only the ethanolic extract obtained from Myracrodruon urundeuva seeds (EEMUS), which has steroids, alkaloids and phenols, showed in vitro cytotoxic activity against human cancer cells, being 2-fold more active on leukemia HL-60 line [IC50 value of 12.5 (9.5-16.7) μg/mL] than on glioblastoma SF-295 [IC50 of 25.1 (17.3-36.3) μg/mL] and Sarcoma 180 cells [IC50 of 38.1 (33.5-43.4) μg/mL]. After...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antiproliferative potential; Northeastern Brazilian plants; Myracrodruon urundeuva; Sarcoa 180 tumor; Seed extracts.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000300023
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Larvicidal activity of the water extract of Moringa oleifera seeds against Aedes aegypti and its toxicity upon laboratory animals 42
Ferreira,Paulo M.P.; Carvalho,Ana F.U.; Farias,Davi F.; Cariolano,Nara G.; Melo,Vânia M.M.; Queiroz,Maria G.R.; Martins,Alice M.C.; Machado-Neto,Joaquim G..
In this work, biological effects of the water extract of Moringa oleifera seeds (WEMOS) were assessed on eggs and 3rd instar larvae of Aedes aegypti and on its toxicity upon laboratory animals (Daphnia magna, mice and rats). Crude WEMOS showed a LC50 value of 1260µg/mL, causing 99.2 ± 2.9% larvae mortality within 24 h at 5200µg/mL, though this larvicidal activity has been lost completely at 80ºC/10 min. WEMOS did not demonstrate capacity to prevent egg hatching. After extensive dialyses of the crude WEMOS into watersoluble dialyzable (DF) and nondyalizable (NDF) fractions, only DF maintained its efficacy to kill larvae. Acute toxicity evaluations on daphnids (EC50 of 188.7µg/mL) and mice (LD50 of 446.5 mg/kg body weight) pointed out to low toxicity....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Daphnia magna; Larvicidal activity; Moringa oleifera seeds; Toxicity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000200007
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A brief report on some health aspects of rats fed with crescent levels of recombinant chagasin, a potential plant defense protein 42
Oliveira Neto,Osmundo B.; Farias,Davi F.; Vasconcelos,Ilka M.; Paes,Norma S.; Monteiro,Ana C. S.; Silva,Maria C. M. da; Guimarães,Luciane M.; Carvalho,Ana F.U.; Grossi-de-Sá,Maria F..
Chagasin may be considered a potential plant-incorporated protectant (PIP) protein due to its deleterious effects on insect pests. However, extensive safety studies with PIP's are necessary before introducing them into the target plant. Thus, a short-term feeding trial in rats with high doses of r-chagasin was conducted to provide evidences about its safety. Three test diets containing casein + r-chagasin (0.25, 0.5 and 1% of total protein) were offered to rats (10 days). The test diets did not show adverse effects upon the development, organ weight, hematological parameters and serum protein profiles of rats, providing preliminary information on the safety of r-chagasin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chagasin; Recombinant protein; Hazard potential; Short-term rat feeding trial.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000100019
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