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| Dianese,Érico de C.; Ramalho,Eduardo D.; Cerqueira,Daniela M.; Lopes,Daniela B.; Fajardo,Thor V.M.; Ferreira,Marisa A.S.V.; Martins,Cláudia R.F.. |
| Many viral diseases, including leafroll, which is of great economic importance, affect grapevines (Vitis spp.). A complex of eight viruses [Grapevine leafroll-associated virus (GLRaV) -1 to 8] is associated with this disease. The objective of this study was to compare the variability of the 3' terminal region of the polymerase gene of three isolates of GLRaV-3 (Grapevine leafroll-associated virus-3), from Submédio do Vale do Rio São Francisco (Petrolina-PE) with that of other isolates available at the GenBank, including an isolate from North America and another from Southern Brazil. The viral RNA was extracted from three infected ELISA reactive plants and a fragment of 340 bp was amplified, by RT-PCR, using primers that recognize that portion of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GLRaV-3; Vitis spp.; Ampelovirus; RNA dependent RNA polymerase. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200012 |
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| Fajardo,Thor V.M.; Dianese,Érico C.; Eiras,Marcelo; Cerqueira,Daniela M.; Lopes,Daniela B.; Ferreira,Marisa A.S.V.; Martins,Cláudia R.F.. |
| Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Closteroviridae; Ampelovirus; GLRaV-3. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400008 |
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