Data/hora: 27/07/2024 02:42:49

Provedor de dados: 	Genetics and Molecular Biology	
País: 	Brazil	
Título: 	A two-step strategy for the complementation of M: tuberculosis mutants	
Autores: 	Movahedzadeh,Farahnaz; Frita,Rosangela; Gutka,Hiten J.	
Data: 	2011-01-01	
Ano: 	2011	
Palavras-chave: 	Tuberculosis; Mutagenesis; Constitutive expression; Complementation.	
Resumo: 	The sequence of Mycobacterium tuberculosis, completed in 1998, facilitated both the development of genomic tools, and the creation of a number of mycobacterial mutants. These mutants have a wide range of phenotypes, from attenuated to hypervirulent strains. These phenotypes must be confirmed, to rule out possible secondary mutations that may arise during the generation of mutant strains. This may occur during the amplification of target genes or during the generation of the mutation, thus constructing a complementation strain, which expresses the wild-type copy of the gene in the mutant strain, becomes necessary. In this study we have introduced a two-step strategy to construct complementation strains using the Ag85 promoter. We have constitutively expressed dosR and have shown dosR expression is restored to wild-type level.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200020	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572011000200020	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.34 n.2 2011	
Fonte: 	info:eu-repo/semantics/openAccess