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Cryotop and development of vitrified immature bovine oocytes Arq. Bras. Med. Vet. Zootec.
Hajarian,H; Wahid,H; Rosnina,Y; Daliri,M; Dashtizad,M; Karamishabankareh,H; Abas Mazni,O.
The effectiveness of different cryodevices (open-pulled straw (OPS), electron microscopy grid (EMG), and Cryotop was evaluated for vitrification of immature bovine oocytes. Polar body, metaphase II stage (MII), survivability, and subsequent developmental rates were determined. Only oocytes with four or five layers of cumulus cells were used. Oocytes were equilibrated in two vitrification solutions - 1: 10% DMSO + 10% ethylene glycol (EG) for 30-45sec and 2: 20% DMSO + 20% EG +0.5M sucrose for 25sec -, mounted on one of the cryodevices and directly plunged into liquid nitrogen for 10 days. Immature vitrified oocytes using Cryotop showed the highest rates of polar body extrusion (PB) and nuclear maturity (MII); 41 and 58% respectively. Vitrified oocytes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Immature oocyte; Vitrification; Cryodevice.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000100011
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