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Data/hora: 26/07/2024 23:13:51

Partial Purification and Characterization of β-glucosidase fromMonascus sanguineus
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Dikshit,Rashmi; Tallapragada,Padmavathi.
The aim of the present work was to study the production and characterization of β-glucosidase from Monascus sanguineus. Agro-waste residues were screened to obtain the maximum yield of enzyme. Jack fruit seed was the best substrate for enzyme production. Studies on the optimization of pH and temperature showed acidic pH favorable for enzymatic activity, whereas the optimum temperature was 60°C. Enzyme kinetics studies with different concentration of pNPG showed the calculated value of Km approximately 0.89 mM with the non-linear regression and 0.98 mM with the linear regression techniques. The enzyme was predominantly inhibited by KCl (69.8%) and moderately inhibited by CaCl2(14.8%). Studies on the sensitivity for glucose showed that after 100 mM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zymography; Agro-waste; Kinetics; Monascus sanguineus; PNPG.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200185