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| FAJARDO,THOR V. M.; NISHIJIMA,MARTA; BUSO,JOSÉ A.; TORRES,ANTÔNIO C.; ÁVILA,ANTÔNIO C.; RESENDE,RENATO O.. |
| Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: OYDV-G; LYSV; GCLV; Detection; Coat protein; PCR; Probes; Epidemiology. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300007 |
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| NICKEL,OSMAR; FAJARDO,THOR V. M.; ARAGÃO,FRANCISCO J. L.; CHAGAS,CÉSAR M.; KUHN,GILMAR B.. |
| An isolate of Grapevine virus B (GVB), obtained by indexing Vitis labrusca and V. vinifera grapevines on the indicator LN33, was transmitted mechanically to several Nicotiana species. The virus was partially purified from N. cavicola and the coat protein estimated at 23 kDa by SDS-PAGE. In negatively stained leaf extracts of experimentally inoculated N. cavicola and N. occidentalis, flexuous particles with cross banding were observed, predominantly measuring 750-770 x 12 nm, with a modal length of 760 nm. Decoration indicated a clear, positive reaction against AS-GVB. In DAS-ELISA, GVB was detected in N. cavicola and grapevine extracts, and Western blots showed homologous and cross reaction of GVB and GVA antisera with GVB coat protein. Using specific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GVB; Vitis spp.; Vitivirus; Electron microscopy; ELISA; Western blot; GVA. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300007 |
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