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Gene expression profiling reveals upregulated FUT1 and MYBPC1 in children with pancreaticobiliary maljunction 56
Guo,Wan-Liang; Geng,Jia; Zhao,Jun-gang; Fang,Fang; Huang,Shun-Gen; Wang,Jian.
Pancreaticobiliary maljunction (PBM) is associated with high risk of epithelial atypical growth and malignant transformation of the bile duct or gallbladder. However, overall changes in genetic expression have not been examined in children with PBM. Genome-wide expression was analyzed using peripheral blood samples from 10 children with PBM and 15 pediatric controls. Differentially expressed genes (DEGs) were identified using microarray. Bioinformatics analysis was conducted using Gene Ontology and KEGG analyses. The top 5 in the up-regulated genes in PBM were verified with qRT-PCR. Receiver operator characteristic curve analysis was conducted to evaluate the predictive accuracy of selected genes for PBM. The microarray experiments identified a total of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pediatric; Pancreaticobiliary maljunction; Gene expression.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800603
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Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production 69
Yang,Weiping; Meng,Fanxu; Peng,Jiayin; Han,Peng; Fang,Fang; Ma,Li; Cao,Binyun.
Background The Tibetan pig is a pig breed with excellent grazing characteristics indigenous to the Qinghai-Tibet plateau in China. Under conditions of barn feeding, 90% of its diet consists of forage grass, which helps meet its nutritional needs. The present study aimed to isolate and identify a cellulolytic bacterium from the Tibetan pig's intestine and investigate cellulase production by this bacterium. The study purpose is to provide a basic theory for the research and development of herbivore characteristics and to identify a source of probiotics from the Tibetan pig. Results A cellulolytic bacterium was isolated from a Tibetan pig's intestine and identified based on morphological, physiological, and biochemical characteristics as well as 16S rRNA...
Tipo: Journal article Palavras-chave: Carboxymethyl cellulose; Cellulolytic bacterium; Fermentation conditions; Screening and identification; Tibetan pig.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600002
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Molecular characteristics and virulence gene profiles of Staphylococcus aureus causing bloodstream infection 55
Li,Xuehan; Fang,Fang; Zhao,Jin; Lou,Ning; Li,Chenglin; Huang,Tao; Li,Yirong.
ABSTRACT Background: The rate of methicillin-resistant Staphylococcus aureus (MRSA) among the total of S. aureus isolates decreased to 35.3% in 2017 in China. It is unclear whether the molecular characteristics of S. aureus isolates have changed as the rate decreased. Objective: This study aimed to investigate the molecular characteristics and virulence genes profile of S. aureus isolates causing bloodstream infection and analyze the correlation between the prevalence rates of the common sequence types and MRSA. Methods: A total of 112 S. aureus strains from eight hospitals of four cities, including 32 MRSA isolates, were identified and evaluated through multilocus sequence typing, spa typing, and determination of virulence genes. Results: Twenty-five...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Methicillin-resistant Staphylococcus aureus (MRSA); Bloodstream infection; Virulence encoding gene; Multilocus sequence typing; Spa typing.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000600487
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